LOCUS       DAOOCO010000001        40539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_705_length_40539_cov_5.426736, whole genome shotgun sequence.
ACCESSION   DAOOCO010000001 DAOOCO010000000
VERSION     DAOOCO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 40539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40539
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_40539_cov_5.426736"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..2071
                     /gene="acs"
                     /locus_tag="PK154_00005"
     CDS             173..2071
                     /gene="acs"
                     /locus_tag="PK154_00005"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPW09474.1"
                     /translation="MAESFEVSMAGDEKYYIPDPSYKEQAWTKDYAQAYKEFLADKAG
                     FWERQAHHLEWFKKWDKVLEWNYPYAKWFVNAKLNITYNCLDRHVNNHRRNKVALIWR
                     GEEGEERIFTYKKLFHEVCRFANGLKKLGVTKGDRVCIYMPLVPEQVIAMLACARIGA
                     VHSVVFGGFGVNALNMRINDAEAKIVITADVTIRRGKTIPLKTIVEEAIINSPSVEKV
                     VVLSREVDQPVELHKELEIDYYELMEGVSAECTPEVMDAEDPLFILYTSGSTGTPKGI
                     VHTCGGYMVGTYYTTLNVFDVKDTDVYWCTADTGWITGHSYVVYGPFAVGATVVLAEA
                     VPDYPDAGAFWKIVEDLGITIFYTAPTTIRMFMRVGEEWPNKYDLSSLRILGSVGEPL
                     NPEAFEWYYRVIGKNHCPVVDTWWQTETGMHMVATMVGEPMRPGFAGKSLPGVVADVV
                     DKTGTPVAPGNGGFLVMREPWPAMLRTVYKNDERYRTYWNTIPGCYMAGDLAVKGKDG
                     NIMIIGRADDIIIVAGHNIGTAEVESALVSHQAVAEAAVIGKPDPLKGNLIKAFVILR
                     VGFQPSEKLKSDLIYHVRMTLGPIAMPSEIEFMDKLPKTRSGKIMRRVLKAKEMGIDP
                     GDVSTLEE"
     gene            2100..3350
                     /locus_tag="PK154_00010"
     CDS             2100..3350
                     /locus_tag="PK154_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPW09475.1"
                     /translation="MAPEESLFGMPAERGRWGLVIVGMIINLCLGTIYSWSVFVGPLT
                     SYFQDTLGLTVTANEILMPFSVFLAFFAIAMTLTGKYIESIGPRKITIIGGILTGLGW
                     LLASTVTSVWMLYIVYGIIGGIGVGIAYGVPVAVAARWFPDRRGTAVGLTLLGFGFSA
                     FITANIAGYLIGTTGVMNTFRIFGIVFMILIVLLALPLRFPAEGWRPAGWVPPVPKKG
                     EELCECNRSAMIRTSSFYGLWFCYFIGCLAGLMAISIAKPVGTEIGIATGLATLLVGF
                     FAIFNGGGRPVFGALTDRFNPRNTAILSFVLIALASSLLWQMPSVPVYILAFAILWGC
                     LGGWLAIAPTATGNYFGTCDYPRCYGVVFLAYGAGAIAGPQLAGFIKTTTGNYLGVFP
                     YVLILALLGILVAMLLMKPPKTRE"
     gene            3633..4262
                     /locus_tag="PK154_00015"
     CDS             3633..4262
                     /locus_tag="PK154_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPW09476.1"
                     /translation="MAGKEGIGKSRLEALSDGIFAFSMTLLVISLTVPLVPDWDAPAL
                     LPGMIADMFPEFLIFSIAFFIIAGYWLAHHRILRSIDYVDDRLIWINLLLLFFIVLIP
                     FTTSISGDYNAVLEAVLLFHINLLCASIALTLIWLYIRNQYANLTNDTEPERGRIQFD
                     TAGRIRALAIPAITIVAIFVSFINPAGSMWCYLLIPILMVAGRKAAQLA"
     gene            4278..4829
                     /locus_tag="PK154_00020"
     CDS             4278..4829
                     /locus_tag="PK154_00020"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPW09477.1"
                     /translation="MISNNPGTPREYGLPPLAGEDPLVLILGSFPSKMSIQRQQYYAN
                     PHNQFWRIMADLLNNGDMGDIRSLSRLLTDHHIALWDVIASRRYQNGSMDRDITDPVF
                     NDIPAFLAGHSRIRSIGTNGGTAWVSFQRLVRCTPLESALLCRRLPSTSPAYASCRYE
                     QKLSQWRMFLEGNTEEPSAAAHR"
     gene            5074..5292
                     /locus_tag="PK154_00025"
     CDS             5074..5292
                     /locus_tag="PK154_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW09478.1"
                     /translation="MKTRMKEYRARRDMTQADLADAVGVRRETIVFLERGRYNPSLKL
                     AHAVARELGTTIDNLFLFEEDIDVVLEE"
     gene            5380..6003
                     /locus_tag="PK154_00030"
     CDS             5380..6003
                     /locus_tag="PK154_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09479.1"
                     /translation="MKNLVFPVLGILLLALVLPSSASYSLQEVSVIPPSDATPPGSPL
                     NASAVLLIVPAGPTTYIEGYSLVLSTDLDRAAWNARVLVDGHQAAVFEKSGSTVFISG
                     YLLSYPLSSDVEVDVTLKGTVPTDGAGDPITVLRVLELNNQGEVVPGSETVVTRVLEL
                     PQTHSSPPVPSESHAIPSPTRAGPGISNVAVIAVIALAVLFAGKRAP"
     gene            complement(6207..7169)
                     /locus_tag="PK154_00035"
     CDS             complement(6207..7169)
                     /locus_tag="PK154_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPW09480.1"
                     /translation="MPPALASLPVLLTYLFEAVVLLTLGILLASILVETGIFSRLNGL
                     TRPLCRISGLSHESTTSLLAIFINPMAGKAMYAGFYYDEKVGKEEIIPTLVMSTFPIV
                     LGESLFRVHLPAAVVLLGPVIGTMHVLLNLFSSALQTLGAILYTRLVLHKKVAQRSCE
                     TTPASEGEVSFSRCKIVGGIRKAWPQLIRIIPVTVVAVLIFTILTVYGVMDLIGIFFE
                     PILGAIGLPGESSTALVAQILRSYAGYAVVASLVNTGILVVKTALMTLLVGSMMVITL
                     IYLRYTLPLNLSLFGTFGLKITAVSYTCSMTAKLITLGLVILLL"
     gene            7306..8040
                     /locus_tag="PK154_00040"
     CDS             7306..8040
                     /locus_tag="PK154_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPW09481.1"
                     /translation="MQPTGATFMELTQYDHLSPSDQNMKKTPPPLAKAVPKGAQYIPL
                     PRPGTITVNPMDLRHAIEARRSLRHYQKEPISIEELSYLLWCTQGIVQVVDPFYTLRN
                     VPSAGGRHALETYLLINRVTSLPPGLYRYVPFSHSLIEIDADLSITDKVMAACLGQAF
                     VRSSAVTFLWSTVIYRMAWRYSERAYRLVHLDAGHACQNLYLAAEQVGCGACAIGAYD
                     DELMAELLGLDGKEEFVLYCAAIGKR"
     gene            8192..8563
                     /locus_tag="PK154_00045"
     CDS             8192..8563
                     /locus_tag="PK154_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW09482.1"
                     /translation="MPTIVHFDVPADDVPRARAFYSGLFGWTFTAPPGFPDFYLIETR
                     DLEGKPATGGGMGKRGSPDQRIMNYFGVPDIDEYASRVKRLGGSMVMQKTQVPGFGHL
                     AICADTEGNPFGLWQEDPGAE"
     gene            8580..9305
                     /locus_tag="PK154_00050"
     CDS             8580..9305
                     /locus_tag="PK154_00050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW09483.1"
                     /translation="MDGGAVPALIQSMKGPGEDLLIIGISGSPREMRSRTRMLLQWVL
                     AGAAAAGAKTELIDLTSLRIEACTACESCSLTGACVHTDDFPFLYERMIAAHGIVLGS
                     PVYIDHVTGQMKVFIDRLADAIHYQTLSGKYGCAVATTWSSGGEEVVSYLNHVLNYLG
                     ILALEGISVATGDDPDSLFGAEERARNLGAMLTLAIRTQVPYPGQSAIIGENRAFFAR
                     IVEENREWRQDEYDAWVRKGWIS"
     gene            9355..9870
                     /locus_tag="PK154_00055"
     CDS             9355..9870
                     /locus_tag="PK154_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPW09484.1"
                     /translation="MNLERFDQMNAREKHEYIEFLLWHYRVVDAFWFLYCTDEFGQPV
                     AESLNEKVWGRVASMAAKDIRNRFGITEKGLTGFVRALRLFPWAIIVDYEIEEKPGEV
                     IIRVPSCPTQEARLRRGLSEYVCREMHRAEFTSFAREIDERIVVECLFAPPDPHPPDM
                     FCQWRFTIREE"
     gene            complement(10358..11482)
                     /locus_tag="PK154_00060"
     CDS             complement(10358..11482)
                     /locus_tag="PK154_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HPW09485.1"
                     /translation="MTVTIGFLVNPIAGMGGAVGLKGTDGLLSEAIKRGARPEAGNRA
                     LVALRKLKGAPLRFLSCSGPMGEDPLNVAGITGFEVVYQTPHPTTSEDTRRACAVFEV
                     HGVDLILFCGGDGTARDVYDTVLDRIPILGIPAGVKMYSAVFAITPESAAAIILNLSQ
                     SVRHMRDAEIVDVDEEAYRDGDLRTRLHGYAQSLYLPGLVQGTKQVLEDQDEDRAKEG
                     IGRFLAEVILGTPDILYLIGPGSTTGAIAREFGIPKTILGFDAVKQGRLVASDLNEEE
                     IIRLLEREKKSRLIVSIIGAQGSVLGRGTRQVSPRVIRMIGIENIIVVATPHKLSETP
                     VLFVDTGDEALDRAFGPHISVISGYQIAQRKRIGVLTTQE"
     gene            complement(11540..12898)
                     /locus_tag="PK154_00065"
     CDS             complement(11540..12898)
                     /locus_tag="PK154_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPW09486.1"
                     /translation="MQTKIILDEEEMPKRWYNIQGDLPSPLDPPLNPQTKNPATPSDL
                     AAIFPMELIRQEMSADRYIDIPGEVQDVLRLWRPTPLYRATRLEKALKTPAKIYYKWE
                     GVSPAGSHKPNTAVPQAYYNMKEGVERIATETGAGQWGSALAFATMLYDIQCSIYMVR
                     SSYTQKPYRKSMMNVWGAECIPSPSTKTNAGRAMLEKDPDTPGSLGVAISEAVEDAAT
                     HSNTHYSLGSVLNHVCLHQTIIGLECRAQLASVESYPDIVIGCVGGGSNFAGVSFPFA
                     GDKLIGKHPEVEIIGVEPASCPTLTKGLYAYDYGDEAGLTPIMKMYTLGHDFIPPSIH
                     AGGLRYHGDSPIVSRLVHDGVMRAVSYHQSEVFDAAQTFARSEGFIVAPETSHAVKAA
                     IDEALKCRKSGEEKTILFNASGHGDFDMSAYDAYYAGNLSDYEYPDSLIREALSRLPK
                     VP"
     gene            13062..14390
                     /locus_tag="PK154_00070"
     CDS             13062..14390
                     /locus_tag="PK154_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09487.1"
                     /translation="MAEQKAAEDQHRQEGDSWRIPASINDQWNFLLKTIWDQMIHLVI
                     QFEGAVDLPTLEQAVREAMAAEVLTIAKFSPGDSPFFEPAFPGSDTPSWDVITTAHPD
                     IALSSLLGSSLDPLKGPMCRVRLIRSDNDLLCISFNHTMTDAYGVKSFGSLLANLYRS
                     GTGKGMNPPLGSPHDRSLKSILSLFSGDELATAARRIRKREGAWNIPFESLNVGEGQY
                     VKQTIGQPGFSSVRQFARNHSVTINDLLLASFILALAKMAPPLPNTCNPVLTSIDLRR
                     YQALSFHHALSNFSVAFELPVMIPDHIDPTDVVRHIHTLMASCKSGHAGIGAAALLET
                     EFDKGFWKVEEKLAEMEGKTRQGLSGKNPFFTNLGVIPEGVVDYGIPVRDAWMLGPVE
                     YPPGLCLAASTFRDCLTLSMGYSGTALQKEWVISLLSAMAATLSRFTSWE"
     gene            complement(14374..15894)
                     /locus_tag="PK154_00075"
     CDS             complement(14374..15894)
                     /locus_tag="PK154_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW09488.1"
                     /translation="MHRRYGIIVGFIVFLVIAFIPLDPVTFPYPAQLAAAVTGLMVVW
                     WITEAIPLYATALVPLVLFPILGILSPKEAAVSYADQTVFLFMGGFLIAAAMMRWNLH
                     RRIALNVISLAGSGTKKLVLGFMVATAFISMWISNTATAMMMIPIALALVTTLLPGVL
                     TDKNSEKGGERQFATCLLLGVAYAATIGGMATLIGTPPNGILVAQLSSIFPAAPEIDF
                     FSWMIFAVPFMVIFLFIAWFWLTSVTHRHMPKVISGSRDMIGHELSDLGPLKSGEKWT
                     LLIFLSVALAWIFRTPKEIGSLVIPGITTYIPGITDATIAICGALILFMLPVDTKKGI
                     FTLDWESAKGIPWGILILFGGGICLSEGIIASGLAEVIANSFTLLAVLPLIVIILLIS
                     LFITFLNEVVSNTAMASIMVPLMAITSVSMGINPLMLMVPAALASSLGFMLPVGTPPN
                     AIAYGTGYLSTREMARAGLALNVIGCILITVFMTLVIPTVLGISPELPAWAVIPTK"
     gene            complement(15948..16433)
                     /locus_tag="PK154_00080"
     CDS             complement(15948..16433)
                     /locus_tag="PK154_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW09489.1"
                     /translation="MLGDTHDNLPHIKKAVKVLNREAADRYLHTGDFISPFVIPVLAT
                     LAGEVSGVYGNNDGDHDLLRQRCRETGNVSIAGTFSEFHADGVRIALLHGHEGAILDV
                     IVESGLYDVVVHGHSHRAGIAEKGPTLVINPGEVCGYLTGSSTCAMYDTERREGWIIQ
                     I"
     gene            16560..18011
                     /locus_tag="PK154_00085"
     CDS             16560..18011
                     /locus_tag="PK154_00085"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW09490.1"
                     /translation="MNVHPLSMQMRVGGKEISSADERWIPVINPATGEVIDQVPEGTL
                     HEVDEAVSAAQAAFDSWGKKTVRERGLILYRAAGNVREQHQELARLLTMEQGKPLRDA
                     VDEIRGFANILEFFAGISGAMNGDFIALGSLGDALVKREPIGVCGAIIPWNMPAIIMG
                     WKVAPALLAGNTLVFKPSSSAPLTCLSLASSLERSGLPPGVLNVVTGKGEVVGEGIAR
                     HPAIRKISFTGDRATGFRIQELARSHLKDISLELGGSDPMIVWKDADIGKAVSGVIKG
                     RFYNAGQTCTAVKRLYVHHDIAKEFVVRLKNKVEAIRVGNGLDPGSDMGPLSGKPQLD
                     NVMSFVNTVEKKGQGTILTGGNVMTGGTHGKGFFYAPTLVIDTTPECILLQQEVFGPV
                     LPVVPVNDLETAIAKANSTPYGLGASIWTNDLSVVHEVFSSVAAGVVWVNRHLTIPPE
                     VPFGGVKESGIGRENGIAAYHTYTRTKTLLLNW"
     gene            complement(18276..19442)
                     /locus_tag="PK154_00090"
     CDS             complement(18276..19442)
                     /locus_tag="PK154_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW09491.1"
                     /translation="MHSLMFFELAQRNVRLHWLRSLLAILGIIIGVVTIVSMGILGNS
                     LVLAISDSLSTVGDSIIVTPHVGGMGFGGSQSSNLLSDRDVEQIRRAAAPHVTIPVYS
                     GSSRMKIGSEDTVGTIYGLKPDDIPVLLEVDQGSYLRGASGAMVGATFAEENEIKVGS
                     RISLGEMGSVRVVGILKERGMGFDINPDFGIVVEDQWFKQAFGQEEYNQVIVKVNDLN
                     KIEEVKDSIDRTMNRKETVVDVIDTRAILETILTTFGQISTFTTVIGGISLIVAGVSI
                     LNIMLMSVTERIKEIGVIRSIGTQRKEVLRMFLYEALILGLIGSSIGGALSIIGGYAV
                     SMVMLQTTEYLFVPSSLVYIVYGVGFGLGTSLISGLYPAWKASNLNPIEALRHE"
     gene            complement(19487..20188)
                     /locus_tag="PK154_00095"
     CDS             complement(19487..20188)
                     /locus_tag="PK154_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09492.1"
                     /translation="MEETIDPECDGDTIIRFREVTKVYPLPGGDVVALDKVSLDIGKG
                     DFIAIMGPSGSGKSTLLNLMGCLDTPTAGTLCIRGKNIRGMSDEDLTILRRDYLGFIF
                     QQFNLLPLLTALENVQYPLILKTGSRECRERCRVVLNAMDLDESLFSHKPSEMSGGQQ
                     QRVAIARALVNDPEILLADEPTGNLDTKTGAAIMHLLDDLNRDRGKTIIMVTHDPSIA
                     EYAHRVIRIVDGKIS"
     gene            complement(20173..21315)
                     /locus_tag="PK154_00100"
     CDS             complement(20173..21315)
                     /locus_tag="PK154_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09493.1"
                     /translation="MKTGILLVLFGLIAFSPFISLAGAQQAGEGAAQVTVSSVDLNPG
                     VFFEGDTGIVMVEIINNGLESVAIRRATMYDADISILSSSYDTTTTVGAGNRMQFTFT
                     IKADVPPGIYYPSFSLDFRDAGYLRYPVQVRVENDPIEISVLHKPDTYSAGRKEQIDI
                     MVGNPRDNAVGSVIVHFRGTSLDPAPSSYFIGTLDPDKAQKISFNITPAEPTSLEIAA
                     DYKNGINPHSSMVTLPIVFGESKRQADPILSNILVEYENGKYTLTGDVTNAGLEVANS
                     VVLSAGEGAEPVNPYREYVVGSLQPDDFSSFELTFLAENTSKVHMNVAYRDVDGNPYQ
                     RTTPVVIPPKNNAREDSPGIAPAMIAVILVSAAVIGGAIWYSWKKR"
     gene            complement(21315..21995)
                     /locus_tag="PK154_00105"
     CDS             complement(21315..21995)
                     /locus_tag="PK154_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HPW09494.1"
                     /translation="MLPYLLFRPDTFFSERMKEPEDLKIPGIIVVIGAIISAVTAYLM
                     SGVYSGLFSAAAGEGMASMMGAFSALSAFFAFLILWWIIFAGIFYLLSMAFAGNGTFR
                     RTLEFTGDGLVPVIIGLIISAIVSLYYIPLVEVPVISNIQDPAAVQRFMSDILTDPAF
                     REFTRISAVISVIFLAWSANLWIFGIRHARNLTVRNAAIVVLIPVAIFAIYTLYTAFV
                     GMPFLGGP"
     gene            complement(22293..23228)
                     /locus_tag="PK154_00110"
     CDS             complement(22293..23228)
                     /locus_tag="PK154_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPW09495.1"
                     /translation="MITIYKTGPKNGLETITTFESGAWVHVVSPSQAEIEDLVLRFSI
                     PPDFFTDPLDVDERARIEREEGNTLIVLRTPRREATEADIPFTTLPIGIILTQGLVIT
                     VTLTEVDVVQEFLNGKVRNFSTMNRTRFVILLFLRTSLMFLRYLKDINRTTAAIENDL
                     HKALRNEQLIRLLNMEKSLVFFITSLRSNALMLEKFNTSGCMKMDDDDRDIFEEVVIE
                     NKQAIEMANIYTSILTGTMDAFASIISNNLNVIIKLLTTVTIILMIPTLVASIYGMNV
                     ELPFQHNPYAFLIAIAFSIIASVIGIVFFWRKEFF"
     gene            complement(23512..24645)
                     /locus_tag="PK154_00115"
     CDS             complement(23512..24645)
                     /locus_tag="PK154_00115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW09496.1"
                     /translation="MRELSGIISGIAPSATLEISDKARQMQRQGIDVISLSIGEPDFD
                     TPAHIKEACIQALTCGETHYAPSNGIPELLSAISRKTEQENKITCTPGQVIVTCGAKN
                     AIYDAMVAVLNPGDEVIILDPSWVSYEPCVRMAGGKPVHHLLNSKTFQVDESVLDKIT
                     GKTRMIVINSPSNPSGAILNRSSLGLIADMCEDYDLFSLSDEIYEKLIYGREHISLGS
                     IRDMAERTITVNGFSKAYAMTGWRLGWAVAPQAIINQMSKVQQHSISHPTTFAMYGGV
                     AALTCDQSCVETMRKEFHRRRDYLVQELLLMGYTVAPAEGAFYAYVNTGGDDIGTARK
                     WLEKGLVAVTPGSAFNTPGWQRISYATSMGHLQEAVRRIKSLT"
     gene            complement(24642..25049)
                     /gene="ribH"
                     /locus_tag="PK154_00120"
     CDS             complement(24642..25049)
                     /gene="ribH"
                     /locus_tag="PK154_00120"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPW09497.1"
                     /translation="MNVKLGFVISEFNRDITYMMEVEGREHASFLGAEVIDCMYVPGT
                     YDMPLAVKRMLKKGVVDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFDKPVTLG
                     ISGPGMTRLEANERIDYAKRAVESAVKMCQRLA"
     gene            complement(25118..25579)
                     /gene="ribC"
                     /locus_tag="PK154_00125"
     CDS             complement(25118..25579)
                     /gene="ribC"
                     /locus_tag="PK154_00125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPW09498.1"
                     /translation="MKIGIADTTFARVNMGGIAADELKRNASVTIERVTVPGIKDLPV
                     ACKKLIEERHCGLVMALGMPGGKEKDRMCAHEASTGLITCQLMTNTHIIEVFVHEDEA
                     RDDRELAWLAEQRTREHAKNALKIAYYPQELTKEAGTGQRQGFPDAGPARR"
     gene            complement(25576..26706)
                     /locus_tag="PK154_00130"
     CDS             complement(25576..26706)
                     /locus_tag="PK154_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPW09499.1"
                     /translation="MEKEPLLMLPGPVPVPERVRMAMVRQAINHRGAEFGAVYADCVR
                     VLKETFGTKNELFVISGSGTAAMEAAVANFGSGLTIACLENGKFGERMFKIAQRYGTA
                     KVVRSEWGTPLDLVALEHELAEGAGMVTLVHNETSAGIKNPAEKIGRLARKYDALFVM
                     DGITSIGGDEVHADAWGADIAVVGSQKCLAAPAGLSAISVSERAWERLSERRPYYLDL
                     AAYRKSASAKVMETPYTPAVPLFLALREACLIIEEEGLAARITRHRKMSQAVRSAVSA
                     WGLALFPKLDGDHAYSNTVTATRLPEGVEDSAFRGAIKKMGIEIAGGQDHLKGKIFRI
                     GTMGATGAPEILATLSAVEHALARQGVRLAGNGVRAAAEVLG"
     gene            26763..27170
                     /gene="purE"
                     /locus_tag="PK154_00135"
     CDS             26763..27170
                     /gene="purE"
                     /locus_tag="PK154_00135"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW09500.1"
                     /translation="MVEVAILSGSASDAAIVEKAAAVLEEHGISYEVRVISAHRDPDV
                     LDEYVKKSDCRVFIAIAGLSAALPGVVSSKTERPVIGVPVSGKLMGLDALLSIVQMPK
                     GVPVACVGIDSGENAALLACRILETRRGREKVF"
     gene            complement(27570..28838)
                     /gene="thiC"
                     /locus_tag="PK154_00140"
     CDS             complement(27570..28838)
                     /gene="thiC"
                     /locus_tag="PK154_00140"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPW09501.1"
                     /translation="MRTLIQECIAGIPMIVEPAARQEGQNPRDFARAVARGHVVIPAN
                     PVRPHRLSAIGEGCRVKVNVNVGTSGERCDLALEREKAEAAIANGADTLMDLSTGGDL
                     EAIRKMILSFDVPVGTVPVYEAVRRAGSAVDLSADLLFTVIREHCRQGVDFLTLHCGV
                     NREALASLKSDPRILPVVSRGGAFHVAWMERSGEENPLFSEFDYLLEILAEHEVVVSL
                     GDGMRPGCLQDADRHAKSVEYLNLGRLANRALDAGVQRMIEGPGHIPFDQIGYNVRMI
                     KELTNEAPLYLLGPLVTDIAPGYDHMVAAIGGVEACRHGADFLCMVSPSEHLALPLVG
                     DIVEGTRAAKLSAHIGDLIRGKEGFRMPRERQMADARRRLDWKEQFSLALFPDAARAI
                     HARDGDLDTCSMCGDLCAVKMMQEMFKEKK"
     gene            28875..29477
                     /locus_tag="PK154_00145"
     CDS             28875..29477
                     /locus_tag="PK154_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW09502.1"
                     /translation="MPVTWIPGRGFVANAYLFGNILIDAGVLPSAVDPYREEIDTIVL
                     THCHYDHTAHVKEIAHMCDAKVAIHPLDAPGLFEDHQSLSLHFGARSPGIAAQQLLSE
                     GDRIGPLKVIHTPGHTQGSICLYSEEERFLISGDTVFTDGGFGRYDFPGGDRTALAQS
                     IGKLASLPVEGLYPGHGTPVRYEGRLHISAALQLLKSGYA"
     gene            29494..29910
                     /locus_tag="PK154_00150"
     CDS             29494..29910
                     /locus_tag="PK154_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HPW09503.1"
                     /translation="MFRNRCCTVEVGSLGEVTFPSGWHVYTGSAQGPGGLARVARHIR
                     VKREGIRSPHWHVDYLLVQPGFRLSAVACAPTTAQSDECRISGLLARDPVPGFGCSDC
                     RCRSHLAYFKSNPEKEVKSAFLHAGLDVTITRLNIS"
     gene            29927..30478
                     /locus_tag="PK154_00155"
     CDS             29927..30478
                     /locus_tag="PK154_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW09504.1"
                     /translation="MTVLGISGSMRKDGNTALLVRAVLETCEKSGLKTEFISLAGKKI
                     NPCLGCEKCKEKDWCVVQNDDWGEIMQKVIDAEVLVIGSPTYYFDVCGHLKNFIDRSY
                     SLYHHRKLAGRKAVAVAVCANKGGARTIETIEGFLNSHEFSYLGWVRGKGYLEGDILK
                     DTRALRRAEEIGERIVKLLKPHD"
     gene            complement(31008..32141)
                     /gene="cofH"
                     /locus_tag="PK154_00160"
     CDS             complement(31008..32141)
                     /gene="cofH"
                     /locus_tag="PK154_00160"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HPW09505.1"
                     /translation="MIALSRSSTVNTGKRDERGVRTLLRDILAGYRMSDEDALMLFSV
                     NDRRIWQIAASADEKREKVTGPIVTFVRNQNINVTNYCINSCGFCGFSKKEGEAGGYL
                     SSRDEIRNKAALARQRNVTEICTVSGLHPDFDAESYTGIITTLREGAPGVHIHASNPM
                     EVAYAARKSGISTSEVLQQMKGAGLGSLCGTAAEILVDDVRKVICPGKIPTDEWIRII
                     REAHLLGIPTTATIMYGHCETVEDRVRHLSILRAIQDETGGFTEFVPLSFVHMNTPLF
                     RKGLARPGATGREDLLMTAVSRLYFDNFDHIQVSWVKTGVKMAQIGLMAGADDLGGTM
                     YEESISKGAGAVNTDYLDPAEMKRIALDIGRTLRERDTLYRFV"
     gene            32222..32524
                     /locus_tag="PK154_00165"
     CDS             32222..32524
                     /locus_tag="PK154_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YunC family protein"
                     /protein_id="HPW09506.1"
                     /translation="MKSEEIQLENSMGTGYVLPLGPVTLVWVVARRGLVGCGAFDVAA
                     LGRFGYPAARVRPACGDSVATLEDLVWGVIKEANVPAQKLGISNGMSGKEALDLLS"
     gene            32573..33523
                     /locus_tag="PK154_00170"
     CDS             32573..33523
                     /locus_tag="PK154_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW09507.1"
                     /translation="MKYSHLFGPVHSRRLGRSLGIDLVPVKTCSFDCVYCECGHTTRE
                     TTERQEFFPLREVLEELRTYLSSAPVLDFITFSGSGEPTLSTSIGDVICFLKESYPQY
                     RVAVLTNGSLFWRFDVRNDLMQADLVLPTLSSVFEETFSRIQRPAPGIHVAEVIEGLI
                     QFRREYAGEIWLEVFIIPGINTSRRELEGLRVAIEQIAPDRVQVNTLDRPGTEHWVRP
                     ASPAELERIRGALGISGLIPVEPIRYELTAGAPMTTEWEEAVALVRELIRRRPCTLED
                     ITTATGLSRREILKILREIQISSGIQESQEERGIFFFCPE"
     gene            complement(33886..34983)
                     /gene="cofH"
                     /locus_tag="PK154_00175"
     CDS             complement(33886..34983)
                     /gene="cofH"
                     /locus_tag="PK154_00175"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HPW09508.1"
                     /translation="MDISTILRDVEGGHRLSEEEAAALLKVRGRDIFLIFSLADALRE
                     ERAGTVVTYVRNQNIHLTNICKNLCGFCGFGRNEGDDGAFCDDTETIEKKVALATSRK
                     VTEICLLSGVHPRFTTVTYEEILKTVHRAAPHVHIHAFSPDEVMHAARQDGITTREVL
                     ERMRSAGLSSLQGTAAEILADHVRSVICPRKVSTAEWVGIIQEAHRMAIPTTATIMYG
                     TCETEEDRARHLALLREIQDETGGFTELVPLSYIYQNTRLYQDGIAGPGASGREDLLL
                     TAVARLFLDNFRNIQVSWGKLGVKLGQLALLAGANDLAGTMFSDDVSGDAGAAGSDYL
                     DPAMMEIMVRDIGRPLRQRRTDYSLVEAEQG"
     gene            complement(34999..35874)
                     /locus_tag="PK154_00180"
     CDS             complement(34999..35874)
                     /locus_tag="PK154_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPW09509.1"
                     /translation="MVDPKKKAEEKSLIGTLRTSEHPWIAFAREIIWIVAVVAGIALV
                     LYLASGTWPAVVTIESESMVPHMNVGDLVFVVEKDRYGALQTWQSGRETGYMKFDEYG
                     DVLIYSPNGAEGSLIPGLGGGIHPIIHRAMDTTETSTSIPVYYYFYPGQGSPDRYLPV
                     TIDDNAWILEDGTIVARIQNGLLIPDKGNATPDEGYIVISEQVSKNSGYLTKGDNNVR
                     SDQGSYLSRAELGVIQPVKPEWIVGKAFFAIPLIGYIPLNIKLVAVILILVLVVWEFY
                     QRRKSGESGKKQEKK"
     gene            36018..37415
                     /locus_tag="PK154_00185"
     CDS             36018..37415
                     /locus_tag="PK154_00185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HPW09510.1"
                     /translation="MLDTRTIVHRFLETKMQVHPDVVRYLAEQGDPGLIETIIESIPK
                     DTIVVSVKHIPGIIPDRDGSRFSADPRCEVVKGNNGASGYGGTVTDYVQYFRDRYNRL
                     GSIVRSRCTPVPIEGLLRNSRYREETCTVLGLVIETRSTTNGHRMAELEDPSGTLPVL
                     FHKGRPVFSDGETLIPDEVIGVRGTLSGDGRLFFADQVLRPDVPINHAPFTSSEPGSA
                     VLISDIHVGSDTFLENAWNRFSDWLDDAEVSYLLIAGDLVDGIGVYPGQEHELTIANI
                     YEQYDALGAMLSSLPSRMVIVISPGNHDVVRAAEPQPAIPPEFVGRFPKNCMFVENPA
                     LVSLQGVMVQMYHGRSIDDLISLVPGAKYEDASPMIAGMLQRRHLAPTYGKRTPIAPG
                     REDHLVIDPVPEVLHTGHIHIMGISDYRGTLCVNAGTWQSQTSFQKQMNINPTPARAV
                     QLDLQTLKPTVLDFN"
     gene            37593..39614
                     /locus_tag="PK154_00190"
     CDS             37593..39614
                     /locus_tag="PK154_00190"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPW09511.1"
                     /translation="MDLFILVPICAFIGLLFAAYSYMVLKREEEGSDLMKKIAGAIRL
                     GAMVYLKRQYTAILVFMIAMAIVLALAINPFTAVCFIVGASLSALAGYIGMKAATYAN
                     VRTTNAAKKGMANAFRVSYSSGTVMGLIVVGLGLLGLSVLFYAFSSFTALTLADTVNV
                     LFGFSLGASSIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDIAGMGADLYESYVGSVIATMGLAVVASIAAGFVNVPVQNLVLLPMLIAALGIIAS
                     IIGTFFVRSSKNESSAIHKAFNIGTLTSLVLTVIATWWLVDTLIGPQYIGVFIATVAG
                     LVAGFLIGLVTEYYTSYERPPTQRIVKAAETGAATDIITGLAVGFESTIWPVIIIVAA
                     ILIAANQAGLYGIALAGVGMLATLGITLSVDAYGPVADNAGGIAEMSHQEKGIREITD
                     TLDSVGNTTAAIGKGFAIGGAALTALALFAVYTQAVSLQVVDMLDPIVFVGILIGAVL
                     PFLFCSFAMTAVGRAAGYIVEEVRRQFKQIPGLMEGKAEPDYDSCITISTNAALKQMI
                     APGLMAIIAPVLVGLTLGTAALAGLLVGSISTGFIVAIMMANAGGAWDNTKKYIEAGH
                     LGGKGSFAHKAAVTGDTVGDPFKDTAGPALNILLKLMSIVSLVFAPIFI"
     gene            complement(40037..>40539)
                     /locus_tag="PK154_00195"
     CDS             complement(40037..>40539)
                     /locus_tag="PK154_00195"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPW09512.1"
                     /translation="VFVVIIILSAAAILGDSVNYKIGTSFGNWLIHREKCLVDKRHVE
                     MTRQYYKKYGKKTIVMARFVPGIRSFAPFVAGIIKMRYPAFLFYNVIGGILWVTGFVL
                     FGYLVASLPVFSARQDIFLWAILIISIGIFLMMMYNFRKNMKECVVTPDETLEELTKE
                     IGDKAK"
BASE COUNT         9071 a        11143 c        11103 g         9222 t
ORIGIN      
        1 agaagaacag cgccgacagc gaccggctgg aactgcagtc ccgcatggag gaaaccgttg
       61 cagcggatta tcgttcttcg gtgcatgcgg gacttccatg catccattct gatgcaggat
      121 gttgatgaga cgatcgccca aggatcgtca cacagatgag gagtaacctt acatggcaga
      181 atcatttgaa gtgtccatgg ccggggatga gaaatattat atccccgacc cgtcatacaa
      241 ggagcaggcg tggacaaagg actatgccca ggcttataag gaatttttag cagataaagc
      301 gggattctgg gagagacagg cccaccatct cgaatggttc aaaaagtggg acaaggtcct
      361 cgaatggaac tatccctatg ccaagtggtt cgtaaacgcc aagctcaata tcacttacaa
      421 ttgcctggac aggcatgtca acaaccaccg ccggaacaaa gttgcactca tctggagggg
      481 cgaggaagga gaagagagga tcttcacata caaaaagctc ttccatgagg tctgccgctt
      541 tgcaaacggg ctgaagaagc tcggagtcac gaaaggagac cgggtgtgta tctacatgcc
      601 cctcgtgccc gagcaggtga tcgcaatgct cgcctgtgca cggatcggag ccgttcacag
      661 cgtcgttttt ggaggcttcg gggtaaatgc cctgaatatg cgcatcaacg atgccgaggc
      721 aaagattgtc atcaccgcag acgtgacaat acggcgtggc aagactatcc cgttgaagac
      781 catcgtcgaa gaagccatca tcaattcccc aagcgttgag aaggtagtcg ttctctcccg
      841 tgaggtggat cagccggtag aactccacaa ggagctggag atagactact atgaactgat
      901 ggaaggagta tcagccgaat gcacaccgga ggtaatggat gcagaagacc ccctcttcat
      961 cctgtatacc tccggttcga ccgggacacc gaagggaatc gtccatacct gtggggggta
     1021 catggtcggt acctattaca cgaccctgaa tgtgtttgac gtcaaggaca ccgacgtgta
     1081 ctggtgtacc gcagataccg gctggattac cgggcacagc tatgtcgtgt atggaccatt
     1141 cgctgtcgga gcaacggtgg ttcttgccga agcagttcct gattaccccg atgcaggggc
     1201 cttctggaag atcgtcgagg atctcgggat cacgatcttc tataccgccc cgactaccat
     1261 ccggatgttc atgcgggttg gagaggaatg gccgaacaag tatgacttgt cctccctccg
     1321 gatcctcgga tccgtcggag aaccgctcaa cccggaagcg ttcgaatggt attaccgggt
     1381 catcgggaaa aaccactgtc ctgttgttga tacctggtgg cagacagaga ccggaatgca
     1441 catggtcgcc acaatggtag gggaaccgat gcgtcctggg ttcgctggaa aatccctccc
     1501 cggagttgtt gcagatgttg ttgacaagac ggggacaccg gtagcacccg gaaacggagg
     1561 atttctcgtg atgcgggaac cctggccggc gatgctgcgg acggtataca aaaacgatga
     1621 gcgatatagg acatactgga ataccatccc gggctgttac atggctggcg atcttgccgt
     1681 caagggaaag gacgggaaca tcatgatcat cggcagggct gatgacatca tcattgttgc
     1741 gggacataac atcgggacgg cagaagtcga gagtgccctc gtctcacacc aggcagttgc
     1801 agaggcagcc gtcataggga agccggatcc gctgaaaggg aaccttatca aggcatttgt
     1861 catcctccgt gtcgggttcc agccgagcga gaaattaaaa tccgacctga tctaccatgt
     1921 taggatgacg ctcggcccca tcgcaatgcc ctccgagatc gagttcatgg acaagctccc
     1981 gaagacaaga tccgggaaga tcatgagacg ggtgctgaaa gccaaagaga tggggatcga
     2041 tccaggggat gtttccaccc tggaggaata accacatttt ttctgggggt atgtagtaaa
     2101 tggctcctga agagtcctta ttcggtatgc cggcagagag aggccgctgg ggccttgtga
     2161 tcgtcggcat gatcatcaac ctgtgcctcg gcacgattta ttcatggagt gttttcgtcg
     2221 gaccgctgac cagttatttt caggatacgc ttgggctgac ggtaaccgcg aatgagatcc
     2281 tgatgccgtt ctcggtattc cttgcatttt ttgccatcgc gatgacgctg acagggaagt
     2341 acatcgagag cataggtccc cgcaagataa ccatcatagg gggaatactg accggcctcg
     2401 gatggctgct cgcatcgacc gttacctcgg tctggatgct atacatcgta tacggaatca
     2461 tcggtggcat cggggtcggt attgcatatg gggttcctgt cgcggttgct gcccgctggt
     2521 tccctgatcg gcgtgggact gcggtcgggc tcacgcttct gggcttcgga ttctctgcct
     2581 tcatcaccgc aaatattgca ggatacctta tcggaacaac cggggtcatg aacaccttcc
     2641 ggatattcgg catcgtattc atgatcctga tcgtcctcct ggcactaccg ctccgttttc
     2701 ctgccgaagg ctggagaccg gcgggatggg taccccctgt cccgaaaaag ggtgaagagc
     2761 tctgcgagtg caaccggtct gccatgatcc ggacttccag cttctatgga ctctggttct
     2821 gctactttat cgggtgcctg gcagggctta tggcgatctc catcgcaaaa ccggtcggga
     2881 cggagatagg aatcgccacc ggccttgcaa cactcctggt gggattcttt gccatattca
     2941 atggcggcgg cagaccggtc tttggcgccc tgacggatcg tttcaatcca aggaacacgg
     3001 cgatactctc ctttgttctg atagcccttg catcaagcct gctctggcag atgccatcgg
     3061 tcccggtcta tatcctcgcc tttgccatcc tctggggttg cctcggtggc tggcttgcca
     3121 ttgctcccac ggcaaccgga aattatttcg gaacctgcga ctatccccgg tgctatgggg
     3181 ttgtattcct tgcgtatggg gcaggggcca tcgcgggtcc ccagcttgca ggattcatca
     3241 agacgacaac cgggaattat cttggcgtct tcccgtacgt gttgatcctg gcccttctgg
     3301 gtatcctcgt agccatgctt ctcatgaagc ccccgaagac aagggaataa tctccttttt
     3361 ttccccggtt gttgtcattc tcttcctggc catcgtaatg gagaactggt ggactgacat
     3421 atgacgccag gaatggaaaa cggggaatga aacgtacatc cccaaagggg aagccgcgcc
     3481 attaatcggg gaagaccaga tcctatccca tctgttctct aacgagatct ccctctccaa
     3541 ggtgaatccg gacatgtgtc cgttgcaaca agaaatccag acaataatat ccttgctctt
     3601 cccattctta aaaaaaggct ggtgaaccag gggtggcagg gaaggaaggg attggaaaat
     3661 cgaggcttga ggcgttgagt gacggcattt ttgcgttttc catgacactg cttgtgatca
     3721 gtctgaccgt gcccctcgtc ccagattggg acgcccctgc gctcctcccc ggcatgatag
     3781 cagatatgtt ccccgaattt cttatctttt ccattgcatt cttcatcatt gccggatact
     3841 ggcttgccca ccaccggatc ctccggtcga tcgactacgt tgatgatcgg ctgatctgga
     3901 taaatcttct cctcctgttc tttattgtgc tgattccatt cacgacctct atttctgggg
     3961 actataacgc agtactcgaa gccgtcctcc ttttccacat caaccttctc tgtgcgagta
     4021 tcgccctcac cctcatctgg ctgtatatcc ggaaccagta tgcaaacctg acgaatgata
     4081 ccgagccgga gagagggagg atacaattcg ataccgcagg aaggatccgt gctctggcca
     4141 tacctgccat cacgattgtt gcgatctttg tatcgttcat caatcctgca gggagcatgt
     4201 ggtgttacct gctcattccg attctgatgg ttgccggaag gaaagcagca cagctcgcat
     4261 gatcgcatga ttgaacgatg atctccaata atcccggaac accacgcgaa tatggtctgc
     4321 cccctctcgc tggagaggac cccctagttc ttatacttgg cagtttcccc tccaagatgt
     4381 ctatccagag acagcagtat tatgcgaatc cccataacca gttctggaga attatggccg
     4441 atctcctcaa caacggagat atgggcgaca tccggtctct ttccaggctt ctgaccgatc
     4501 atcatattgc cttgtgggat gtgatcgcat ccagaaggta tcagaacggc agcatggacc
     4561 gggacatcac cgatcccgta ttcaatgata taccggcatt ccttgcagga cactctcgaa
     4621 tcagatctat cggaacgaat ggagggaccg cctgggtctc ttttcaacgg ttagtccggt
     4681 gtactccctt ggagtctgct cttctttgca gacgactccc ttctacgagc cctgcctacg
     4741 catcgtgcag gtacgaacag aaactatcac aatggaggat gttcctcgaa ggaaacacag
     4801 aagaaccgag tgctgcagct caccgctgaa ccccgacctg ccttccgcga gtataaatat
     4861 tccggcgccc cagcctggat tgacggtgag gttagtattg tggtaggttc tccggttata
     4921 tccagagatc aaggttgact gttccttgga taatgttata ttattataac agtagtgaga
     4981 aatagacaac agatagagat tgatcctcct gtaatttcca gcagatccct ttgtggaaag
     5041 gcgatgttcc gggggagaag aaaggtgctt cccatgaaga cgcgaatgaa ggagtaccgg
     5101 gcacggcggg acatgaccca ggcggatctc gctgatgccg taggagtcag gagagagacc
     5161 attgttttcc tcgagagggg gagatacaat ccctccctga aactggccca tgccgttgcc
     5221 agggagcttg ggacaacgat agacaacctc tttctctttg aggaggatat cgatgtcgtt
     5281 ctggaggagt aagcacgggt tccggttctg gccctggctt tggtcccggc ctacccatcg
     5341 aaggacagaa gtatcgcaat cgaagctggt gatgcactga tgaaaaactt agtctttccg
     5401 gttcttggaa tccttctcct cgctctggtc ctgccctctt ctgcgagcta ttcgctgcag
     5461 gaggtctccg tcattcctcc gtccgatgcc acccctcccg gcagcccgct caatgcctca
     5521 gcagtgctcc tgatcgtccc tgccggcccg accacctata tagaagggta cagccttgtc
     5581 ctctcaaccg atcttgacag ggcagcgtgg aatgccaggg tgttggttga cgggcaccag
     5641 gctgcggtgt ttgagaaatc cggaagtacc gtttttatca gcgggtatct cctctcttac
     5701 ccgctcagca gcgatgttga agtggatgtc accctgaaag gaacagtccc cacagatgga
     5761 gcaggagatc caattacagt tctcagggtc cttgagctga acaatcaggg ggaggttgtt
     5821 cccggttcag agacggtcgt tacccgcgtt ctcgaactcc cgcaaacaca ctcttcaccc
     5881 ccggtccctt cagagagcca tgcaataccg tcacctacta gagcaggacc agggattagc
     5941 aatgtggcgg ttattgcagt gatagccttg gccgttctct ttgcagggaa gagggccccg
     6001 tgagatccgg attgtctgaa tgatctcctt ttttgttgat gcgggaacga gcgaagaata
     6061 agcagagggc tcggtcccat tgccactcct gcattgttca gggaaagcaa tggggattgg
     6121 atgaaccgag ccagggattt gccaggggaa aggggacgtt ccccccttca ttttcggttc
     6181 tgaacgggca aacgccaaga tgtctctcat aaaaggagga tcacgaggcc cagcgtgatc
     6241 agtttcgcag tcatgctgca ggtataggag accgccgtta tcttgaggcc gaatgttcca
     6301 aaaagggaga gattgagcgg cagggtatac cgcaggtaga tgagggttat caccatcatg
     6361 gatccgacga gcagggtcat cagggcagtc ttgaccacga ggatcccggt gttcaccagt
     6421 gaggccacta ctgcataccc ggcatatgag cggaggatct gggctaccag ggctgttgag
     6481 ctctcgcccg gcaggccgat ggcaccgaga atcggctcaa agaagatccc gatcaggtcc
     6541 atgaccccat agacggtgag aatggtaaat atgagcacag ctacgacggt gacgggaatt
     6601 attcgtatca gctgaggcca tgctttccgt atacctccca caatcttaca gcgggagaag
     6661 gagacttctc cttcggaagc cggtgtcgtc tcacaggagc gttgggcgac ttttttatgg
     6721 agcaccagtc tcgtatacag aatggcacca agcgtctgga gagcacttga gaagaggttc
     6781 aggaggacat gcatggtccc aatgaccggc cccaggagga ccactgctgc gggcagatgc
     6841 accctgaaga gcgattcacc aaggacgatt ggaaaggtac tcatcacgag cgtggggatg
     6901 atctcctctt ttccgacctt ttcatcatag taaaagcctg cgtacatagc ctttcccgcc
     6961 atgggattga tgaagatggc aaggagggag gtggtagatt catgggaaag tccggagatc
     7021 cggcagagcg gcctcgtaag accgttcaac cgtgagaaga tgcctgtttc aacgaggatg
     7081 ctggcaagga gaatccccag ggtaaggagt acgaccgctt cgaagagata ggtcagcagc
     7141 accgggagcg atgccagggc tggaggcatt tttttggatc atatacgctc tggctggaag
     7201 agacttatga ggatcggtgg gaacggtggg atcggtcggg aacaggagga gaacaggttc
     7261 gtccaatcgg tctctttata tccggtcaca tagcaaccac tcgctatgca gccgacaggc
     7321 gccaccttca tggaactcac gcagtatgac catctctctc cctccgatca gaacatgaaa
     7381 aagacgcctc caccgcttgc gaaagctgtt cccaaagggg cccagtacat ccccctgccc
     7441 aggcccggga ccattaccgt aaatcctatg gatctccgtc acgcaatcga ggcgaggcgc
     7501 tcccttcgcc attaccagaa agagccgatc agtatcgagg aactgtccta tctcctctgg
     7561 tgcacccagg gcattgtgca ggtcgtggac ccgttctata ccctgagaaa cgttccctct
     7621 gcaggggggc gccatgccct tgaaacctac ctgctcatca accgggtgac ctcccttccc
     7681 cccggcctct atcggtatgt accgttctcc cattcactca tcgagatcga tgccgacctt
     7741 tcgatcaccg acaaagtgat ggcggcctgt ctgggccagg cttttgtgcg ctcctcagca
     7801 gtcacctttc tctggtcaac ggtcatctac cggatggcct ggaggtattc cgaacgggca
     7861 taccggctcg tccaccttga tgccggacat gcctgccaga atctctatct tgccgcagag
     7921 caggtggggt gcggggcctg tgccatcggg gcttatgatg acgaactcat ggctgaactg
     7981 ctggggctcg atggaaaaga ggagtttgtt ctctactgtg ccgccatcgg aaagagatag
     8041 ggcctccctg tcgatcaggc cagaagacta ggtcttcatg acccgatacg atacccttct
     8101 tcccccctcc cggcctggaa ttgatgattg aagcagcatg aggggaaaaa cataatacgg
     8161 gatctgcatg agtatcactt cgtgaatcag tatgcctacc attgttcact ttgatgtacc
     8221 ggcagatgat gtcccaaggg cacgggcatt ctattccggg ctcttcggat ggaccttcac
     8281 cgccccgccc ggttttcccg atttctacct gatagagacc cgcgacctcg agggaaaacc
     8341 ggcaaccgga gggggtatgg gaaaacgggg gagcccggac cagcggatca tgaattattt
     8401 tggtgtccct gatatcgatg aatatgcctc gagagtgaag cgactgggcg gatcgatggt
     8461 aatgcaaaaa acgcaggtgc cagggtttgg ccacctcgcg atctgcgctg acacggaagg
     8521 aaatccattc gggctctggc aggaagatcc cggagcagaa taacataaaa ggagcagtca
     8581 tggacggtgg agcggtacct gcactgatac agagtatgaa aggtccggga gaggatctcc
     8641 ttatcatcgg catcagcggg agcccccgcg agatgaggag ccggacccgc atgctcctgc
     8701 aatgggtcct tgccggtgct gcggctgcag gggcaaagac tgagctgatc gatctgacct
     8761 cgctccggat cgaagcctgt accgcatgcg agagctgttc gctgacagga gcctgcgtgc
     8821 atacagacga tttcccgttc ctttatgagc ggatgattgc cgcccatgga attgtgctcg
     8881 gctcgccggt ctatatcgat cacgtcaccg gccagatgaa ggtcttcatc gaccggctgg
     8941 ctgatgccat ccattaccag acgctctcag ggaagtatgg gtgtgctgtc gcgacaacct
     9001 ggagttccgg gggagaggag gtcgtctcct acctgaacca tgtcctcaac taccttggca
     9061 tacttgcact tgaggggatc agcgtagcaa ccggggatga tcccgattcc ctgttcggag
     9121 cggaggagcg agcgcggaac ctgggcgcga tgctcaccct ggccatacgg acacaggtgc
     9181 catatcccgg acagtccgcg atcatcgggg agaaccgggc ttttttcgca cggatcgtgg
     9241 aggagaaccg cgagtggagg caggacgaat acgatgcatg ggtccggaag ggatggatct
     9301 cctgaaccac cggaagtatc ctcacattcc ttctgaacag aaaacggaga tctcatgaac
     9361 ctcgaacgat ttgaccagat gaatgccagg gagaaacacg aatatatcga gtttctcctc
     9421 tggcactacc gtgtcgttga tgcattctgg ttcctctatt gtaccgatga gttcggccag
     9481 cctgttgccg agtcgctgaa tgaaaaggtc tggggccgtg ttgcatcgat ggctgcaaag
     9541 gacatcagga atcggttcgg tatcaccgag aagggactca ccggatttgt cagggcattg
     9601 cggctctttc cctgggcgat tattgttgac tacgagatag aagagaagcc tggtgaggtg
     9661 atcatccggg tgccctcctg tccaacccag gaggcacgcc ttcgcagggg gctctccgaa
     9721 tatgtctgca gggagatgca ccgggcggaa tttaccagtt tcgcccggga gattgatgag
     9781 aggatcgtcg tcgagtgtct ctttgcccct cccgatcccc atcctcccga catgttctgc
     9841 caatggagat tcacgatcag ggaggagtaa ccccttctga tgaatgggta agggccgggg
     9901 tccccgattg aaggaaaatg tacctggacc agggggacct tgcatgatga atgggttagc
     9961 ttgcacacaa tcctgccccc ggaagggatg accagactcc actcttttcc agctcaacag
    10021 ttgatcttac gactctgtct tccccccatg tttgttctca acctcctccc tcagcacaat
    10081 ggtcagtccc caccgttctg accccttcag ctgacatgct ccggggttaa acgggaggga
    10141 agacagggcc ccccacagca tgcactatcg agagagcaga tggtgtgtca tcgaccatcc
    10201 cccttccctg gctgccgacc atcgcacgaa agcaggttat acacccaggg accaatatcc
    10261 tggcccggta agtgatccgg tcatgctgac ggtaggggat aggatcagac ctggagagag
    10321 gaactccatg aagtcacagg ggcgaaaggg gggggattca ctcctgcgtt gtcagcaccc
    10381 caatccgctt cctctgcgcg atctggtacc ccgatatcac tgaaatatgc ggcccgaaag
    10441 ccctgtcaag tgcctcgtcc ccggtgtcga caaaaaggac cggggtctca gacagtttgt
    10501 gcggggtggc aacaacgata atgttctcga tcccaatcat ccggatcacc ctggggctga
    10561 cctgccgggt acctctgccc aggacggatc cctgggctcc aatgatactc acgatcagcc
    10621 gggatttttt ctccctctca agcagccgga ttatctcttc ttcattcagg tctgatgcaa
    10681 caagcctccc ctgcttcacg gcatcaaatc caagaatggt ttttggaatt ccaaattcac
    10741 gcgctatcgc ccctgtggta ctcccaggcc cgataagata caaaatatcg ggggtcccca
    10801 ggatcacttc ggcaagaaac ctcccgatgc cttctttggc ccggtcttcg tcctggtctt
    10861 ccaggacctg ttttgtcccc tggaccagtc caggcagata caggctctgt gcatagccat
    10921 ggagccgggt cctgagatca ccatcgcggt atgcttcctc atccacatca actatctctg
    10981 cgtccctcat atgccggacc gactgtgaga ggttgaggat aatagcagct gctgattcgg
    11041 gcgtaatggc aaagacggct gaatacatct tcactccagc cggaattccc agtataggga
    11101 tacggtcaag gaccgtatca tagacatccc gtgctgtacc gtcaccgccg cagaacagaa
    11161 tcaggtcgac cccgtgtact tcgaatactg cacaggcacg tctcgtatct tctgaggtag
    11221 tggggtgtgg tgtctggtat accacctcaa acccggtgat gccggcaacg ttgagagggt
    11281 cttcccccat cggtcccgaa cagctcagga aacggagggg ggcacccttg agcttccgca
    11341 gtgccacgag ggcccggttc cctgcttccg gccgggctcc cctcttgatg gcttcagaca
    11401 gaagaccatc ggtcccctta aggccaacgg caccacccat tcccgcaatg ggattgacca
    11461 ggaatccaat agtgacagtc atgcaaggac tacgagtgcg gagttacttg tccatggaga
    11521 tggaggagga ggggaatcct catggaactt tcggaagcct gctgagggct tcccgtatca
    11581 ggctgtccgg atattcataa tcggacagat ttcctgcgta gtaggcatca tacgctgaca
    11641 tgtcaaagtc gccatgcccg gatgcgttga acaggatcgt cttctcctca ccgctcttcc
    11701 ggcatttcag ggcttcatcg atcgctgctt tgaccgcatg agaggtctcc ggggcaacga
    11761 tgaacccttc actcctcgcg aaggtctggg ctgcgtcgaa gacctcgctc tgatggtaag
    11821 agacagctct catcacgccg tcatggacga gccttgacac gattggtgaa tcgccgtgat
    11881 acctcaggcc acctgcatgg atggaggggg gaatgaaatc gtgtccgagc gtatacatct
    11941 tcatgatagg agtcagaccc gcctcatcac cataatcata cgcgtagagt cctttggtca
    12001 gcgtcgggca ggatgcaggt tcaacgccga tgatctcgac ctcgggatgc ttcccgatga
    12061 gcttgtcacc ggcaaaggga aacgagactc ccgcaaaatt ggatccgcca ccaacacaac
    12121 ctataacaat atcgggatag gattccaccg atgcaagctg tgcgcggcat tcgaggccaa
    12181 taatcgtctg gtgaagacag acatgattga gcacggagcc aagggagtag tgcgtattgg
    12241 aatgcgtcgc tgcatcctca accgcttctg atatcgcgac gccaagactc ccgggggtat
    12301 ctggatcttt ctcgagcatc gcccgccctg cattcgtttt cgtgctcggg ctcgggatgc
    12361 attcggcacc ccatacgttc atcatggact tccggtatgg tttctgggta taactcgacc
    12421 ggaccatgta gatactacac tggatatcat agagcatggt ggcgaatgca agcgccgatc
    12481 cccactggcc tgcaccagtc tcggtggcga tccgctccac cccttccttc atattataat
    12541 atgcctgcgg aaccgcggtg ttgggtttat ggctccctgc cgggctgaca ccctcccatt
    12601 tgtaatagat ctttgccggg gtcttcaggg ctttctcgag ccgggttgcc cggtagagcg
    12661 gggtcggcct ccagagacgg aggacgtcct gtacttcccc tggaatgtca atgtaccggt
    12721 cagcactcat ctcctgccgt atcagctcca tggggaatat cgcggcaagg tcagaggggg
    12781 ttgcaggatt ctttgtctgg ggattgagtg gaggatccag tggtgacggg agatccccct
    12841 ggatgttata ccagcgcttt ggcatctcct cttcatcaag gatgattttt gtctgcatga
    12901 atgtcgatat gtattgtaca gatatataca ttgttgtata ctttagcgta ttatagagtc
    12961 ggattgctct ttgataatgg ggtaaaaaac cggtaaaggt ggcgtaagtc catcaaaggg
    13021 attgatgtat cctccagact actctatggg aggtatccgc agtggctgaa cagaaggcag
    13081 cggaagatca gcaccggcag gaaggggata gctggcgcat tcccgcatcg ataaatgacc
    13141 agtggaactt tcttttgaag acgatatggg accagatgat tcatcttgtt atccaatttg
    13201 aaggagcagt tgatttacct acactggaac aggctgtcag ggaggccatg gctgccgaag
    13261 tcctcacaat cgcaaagttt tccccgggtg actccccctt cttcgaacct gccttccccg
    13321 ggtccgatac cccttcttgg gatgtcatca caaccgcaca tccggacata gccctttcca
    13381 gcctcctggg atcatcgctc gatcccctga agggtcctat gtgccgggta cggctgatcc
    13441 gatcagataa cgatctgctc tgcatctcgt tcaaccatac catgaccgat gcttatgggg
    13501 tgaaatcgtt tgggagcctc ctcgcaaacc tgtatcgttc cggaacaggg aaaggaatga
    13561 acccccctct gggaagtccg catgatcgct ccctgaagag catcctctcc ctgttttcag
    13621 gagacgaact ggctaccgcc gcccgacgga ttaggaagcg ggagggggca tggaatattc
    13681 ccttcgagtc cttgaacgtt ggggaagggc agtacgtaaa acagaccatt ggacaaccgg
    13741 ggttctcctc tgttcgtcaa tttgcgcgta atcattcagt cacaatcaac gacctcctcc
    13801 tcgcttcatt tattctagca ctagcgaaaa tggcaccgcc actacccaat acctgcaatc
    13861 ctgtgctcac ctcgattgac ctgcgacgtt atcaggctct tagttttcac catgctctct
    13921 cgaacttttc cgtcgcattc gaactgccag tgatgatccc ggatcatatt gatccgaccg
    13981 atgttgtccg ccatatccat accctgatgg catcatgcaa atcaggtcat gccgggatcg
    14041 gcgccgcggc actccttgaa acggaatttg ataagggatt ctggaaggtg gaggaaaaac
    14101 tggcggagat ggaggggaag acacggcagg gactttccgg aaaaaatccc ttcttcacca
    14161 acctgggcgt catccctgaa ggagtcgttg actatggtat tccggtaagg gatgcatgga
    14221 tgctggggcc ggttgaatac ccaccaggac tctgccttgc ggcctcgacc ttccgtgact
    14281 gcctcaccct ttctatgggg tattcaggaa ctgctttgca gaaagaatgg gttatttccc
    14341 tcctctcggc aatggctgcc actctctcgc ggttcacttc gtgggaataa cagcccatgc
    14401 ggggagctca ggcgagattc cgagtacggt ggggatgacc agggtcataa aaaccgtgat
    14461 caggatacac ccgatgacat tcagggcaag acccgcccgg gccatctccc tggtagacag
    14521 gtaccccgtc ccataagcga tcgcattcgg aggagttccg accgggagca tgaagccaag
    14581 cgacgaggca agggctgccg gaaccatcaa catcagtgga ttgatgccca ttgataccga
    14641 tgtgattgcc atcagtggga ccatgatcga tgccattgcg gtatttgaga ccacctcatt
    14701 gagaaaggtg ataaagagag agatgaggag aatgatcacg atcagcggga gcacggcaag
    14761 gagggtgaac gaattggcga ttacctcggc aagcccgctt gcaataattc cttccgagag
    14821 acaaatccct cctccaaata ggatgaggat cccccagggt attcctttcg ctgattccca
    14881 gtcgagggtg aagatccctt ttttcgtatc aacgggaagc atgaaaagga tcaatgcacc
    14941 gcagattgcg atggtggcgt cggtgatccc gggaatatag gtggtaattc ccgggattac
    15001 gagagagcca atctcctttg gggtcctgaa tatccaggca agggcgacag aaaggaagat
    15061 caggagtgtc catttctctc ctgatttgag cgggccgaga tcgctcagct catgcccgat
    15121 catatccctg ctccctgata tcaccttcgg catatgccgg tgggtgaccg atgtcagcca
    15181 gaaccaggcg ataaacagga atatgaccat aaagggcact gcaaaaatca tccatgagaa
    15241 gaaatcaatc tctggcgcag caggaaaaat gctcgacaac tgagcgacaa gaatcccgtt
    15301 tggcggagta ccgataaggg tagccattcc cccgatggtg gcggcatatg cgactccaag
    15361 aagaaggcag gtagcgaact gtcgctcacc acccttttca ctgtttttat cagtcaagac
    15421 tcccgggaga agggttgtga cgagggcgag ggcgatcggg atcatcatca ttgccgtggc
    15481 ggtattggag atccacatcg agataaacgc ggtagcaacc atgaacccca gcacaagctt
    15541 ctttgtcccc gaaccggcaa ggctgatcac attgagggcg atgcgtcggt gaaggttcca
    15601 ccgcatcatg gccgcggcga tcaagaaccc gcccatgaac aggaaaaccg tctgatccgc
    15661 ataggagacc gccgcttctt tgggtgaaag gatcccaaga atcgggaaaa ggaccagggg
    15721 aacaagtgcg gtagcgtaga gcggaatcgc ctcagtgatc caccagacca ccatcagacc
    15781 ggtcactgct gctgcgagct gagcgggata aggaaaggtc acgggatcca gcggaatgaa
    15841 tgcaattacc agaaaaacaa tgaacccgac gatgatcccg taccttctgt gcatgagatg
    15901 aattgttagt cgtaatcacc tatgaacctg tgctatttct cccggtttca gatctggatg
    15961 atccagcctt ccctgcgttc ggtgtcgtac atggcacagg tggaagatcc tgtcaggtat
    16021 ccgcatactt ccccgggatt aatcacaagg gtcgggcctt tctcggcaat tcctgcccgg
    16081 tgcgagtgcc cgtgcaccac gacatcgtat agtccactct cgactatgac atcaagaatg
    16141 gcaccctcat ggccatgcag gagagcaatc cttaccccgt cagcgtggaa ctcggaaaaa
    16201 gtgccggcga tactgacatt tcctgtctcc ctgcaccgct gccggaggag atcatgatca
    16261 ccgtcattgt tcccatacac cccagagact tcccctgcaa gggtcgccag taccgggatc
    16321 acgaacgggg agatgaagtc cccggtgtgg agataccgat ctgcagcctc acggttcagc
    16381 accttcaccg cttttttgat atggggaaga ttatcgtggg tatctccgag catccctatc
    16441 ctcaactatc acacctcagc tcggatattg aattaggggg ggtaaaatgg ttcttgcgat
    16501 cattgcaggc tgcatcggtt cctctcttgt aacggtaacg tcccggtacg gtaaatagga
    16561 tgaacgtaca ccctctctcc atgcagatga gggtcggagg aaaagagata tcctctgcag
    16621 atgagcgttg gataccggtg atcaaccctg ccacggggga ggtgatcgac caggttcccg
    16681 agggaacgct tcacgaggtg gatgaggcag tcagcgctgc ccaggcagcc tttgacagct
    16741 ggggtaaaaa gaccgtccgg gagagggggt tgatcctgta ccgggctgcg ggaaatgtcc
    16801 gggaacagca ccaggaactc gcccggctgc tcactatgga acagggaaag ccgctcaggg
    16861 atgctgtgga cgagatccgc ggatttgcca atattctcga gttctttgcc ggaatatcgg
    16921 gtgccatgaa tggagacttt atcgccctgg gatctctggg tgatgccttg gtaaaaaggg
    16981 agcccattgg ggtatgcggg gccatcatcc catggaacat gcctgccatc attatgggct
    17041 ggaaagtggc acctgccttg cttgcgggaa ataccctcgt cttcaagccc tcgtcctctg
    17101 ccccgctcac ctgcctctcc ctcgcctcgt cccttgagcg atccggtctc ccacccgggg
    17161 tactgaacgt ggtcaccggg aaaggcgagg tggtggggga ggggatcgcc cggcaccctg
    17221 ccatcaggaa gatctcattt accggggaca gggcaaccgg gttcaggatt caggaacttg
    17281 cgaggtctca tttgaaggat atctcccttg aactgggggg gtctgatccc atgatcgtct
    17341 ggaaggatgc tgatatcggg aaggcagtat ccggcgtcat aaagggacgg ttctacaatg
    17401 ccggccagac ctgcaccgcg gtaaagcggc tgtatgtgca tcacgatata gcaaaagagt
    17461 ttgttgtgag gctgaagaac aaggtggagg cgatcagggt ggggaacggt ctggatccag
    17521 ggagtgatat gggtccgttg tccggaaagc ctcaactgga taatgtgatg tcttttgtca
    17581 ataccgtcga aaagaagggg cagggcacca ttctcaccgg aggaaacgtc atgaccgggg
    17641 gcacccatgg aaaaggattc ttctatgcac cgaccctggt aatcgacacg accccggaat
    17701 gtatcctcct ccagcaggag gtgttcgggc cggtgttgcc ggttgtgccg gttaatgatc
    17761 ttgaaactgc catagcaaaa gccaactcaa ccccgtacgg tcttggagcc tccatatgga
    17821 ccaacgacct ttccgtggtc catgaggtgt tctcatccgt tgcggccggg gtggtatggg
    17881 tgaaccgtca tctcaccatc ccgcctgagg ttcctttcgg cggagtgaaa gagagcggga
    17941 ttggaaggga gaatgggatc gctgcatacc atacctatac ccggacaaaa accctgctcc
    18001 tgaactggta atcccgacca tctcttttcc ttcaaccttc ctgaacatca agtcgaggaa
    18061 ttgccgatga aaatgtttcc agaggaaagg atcaggaaga acagggtaaa tcttagagaa
    18121 gaatgagatc cccgtctgac tatcccagat tattccaggt ctttgccgtc cgaagcagac
    18181 tgaaacaact ccaaggggat cccggctggt gcaatggcaa tctgccattg catcagatct
    18241 ctctcctttt ccaggacggg gctatgaagc ggccatcact catgccggag cgcttcaatc
    18301 gggttcaggt tcgatgcctt ccacgcgggg tataatccgg atatgaggct ggtcccgagg
    18361 ccgaacccca ctccgtacac gatatagacg agactggacg gtacaaagag gtattcggtg
    18421 gtctggagca tcaccatgct cacggcataa ccgcctatga ttgagagagc ccctccgatg
    18481 gaactcccga tcaggccgag aatgagggct tcgtacagga acatgcgcag cacttccttt
    18541 ctctgcgttc cgatgctccg gatgacacca atctccttga tccgctcggt gaccgacatc
    18601 agcatgatgt tcaggatgct cacccctgca acgatgaggg atatcccccc gatgacggtc
    18661 gtgaacgtgg atatctggcc gaacgtggtc aggatcgttt caaggattgc cctcgtatcg
    18721 atcacatcga ccactgtttc cttgcggttc atcgttcggt cgatggagtc cttgacctcc
    18781 tcaatcttat tcagatcgtt taccttgaca atgacctggt tgtactcctc ctgcccgaac
    18841 gcctgtttga accactgatc ttcgaccacg atcccgaaat cagggttgat atcaaagccc
    18901 atgccccgct ccttgaggat tccgacgacc cgcaccgaac ccatctcccc aaggctgatc
    18961 cgggatccga ccttgatctc attctcttcg gcaaacgtgg ctcctaccat ggcgccggaa
    19021 gcaccccgga gataggatcc ctggtcaact tcgagcagga cggggatatc atcgggttta
    19081 aggccatata tcgtccccac cgtgtcctct gagccgatct tcatccgcga actacccgaa
    19141 tacacgggga tggtgacatg gggggcagct gcacgccgga tctgttccac atcgcggtca
    19201 gagaggaggt ttgaggactg cgatcctcca aaccccatgc cgccaacatg gggagtcacg
    19261 atgattgaat caccgactgt ggagaggctg tccgagatgg caaggaccag gctgttcccg
    19321 aggatcccca tcgatacgat ggtcacgaca ccgatgatga taccgaggat ggcgagaagc
    19381 gaacggagcc agtggagccg gacattccgt tgtgcaagct caaagaacat cagggaatgc
    19441 atggataccc gtcacctcct ccggattctc ctgcagctcc ggagtatcat gatatcttcc
    19501 cgtcaacgat ccgtatcacc cggtgggcgt attctgctat cgagggatca tgggtgacca
    19561 tgatgatcgt ctttccccgg tccctgttga gatcatcgag gagatgcatg atagctgcgc
    19621 cggtctttgt atcgaggttt ccggtcggtt catcggcaag gaggatctcc gggtcgttga
    19681 ccagtgcgcg cgctatggcg acacgctgct gctgcccccc ggacatctca cttggtttgt
    19741 gtgagaagag cgactcatca agatccatcg cattgagcac taccctgcag cgttccctgc
    19801 attcccggct acctgttttg aggatcaacg ggtactgcac attctcaaga gcggtcagta
    19861 gcggcaggag gttgaactgc tggaagatga aaccgagata gtcacgcctg aggatcgtca
    19921 gatcctcatc gctcatgccg cggatgtttt ttcccctgat acagagggtc cctgccgtcg
    19981 gggtatcaag gcagcccatc aggttgagca gggtagattt gcccgagccc gagggtccca
    20041 tgatggcaat aaaatcccct ttgccgatgt caaggctcac cttgtcgagt gccaccacat
    20101 ccccgccggg aagagggtaa accttcgtca cctcgcggaa ccggatgatg gtgtcaccgt
    20161 cacactccgg gtctatcgtt tcttccatga gtaccagata gcccccccga ttacagcagc
    20221 cgatacaagg atgacagcga tcatggcagg ggcaatacca ggtgagtcct ccctggcatt
    20281 gttctttggc gggatgacta caggagtggt ccgctgatag ggattgccgt cgacgtcacg
    20341 atacgctacg ttcatatgga ctttactggt attctcggca aggaaggtga gctcgaagct
    20401 ggagaaatca tccggctgga gggagcctac gacatactca cggtaagggt tcactggctc
    20461 tgccccttcc ccagcagaga gcaccaccga atttgccact tcgagaccgg cgttggtgac
    20521 atccccggta agggtgtact tcccgttctc atattcgacg agaatattcg agaggatcgg
    20581 atcggcctgt ctcttggact ccccaaagac gatcgggagc gtgaccatcg aagaatgggg
    20641 attgatcccg ttcttgtaat cggcagcgat ctccaggctg gtgggctctg ccggggtgat
    20701 attgaaggag atcttctggg ccttatcagg gtccagggtc ccgataaagt aacttgaggg
    20761 ggccgggtcg agggacgtcc cccggaagtg gacgatcaca ctcccgacgg cattatccct
    20821 cgggtttccc accatgatgt cgatctgctc cttcctcccg gcactgtagg tatcaggttt
    20881 atgcaggaca gagatctcga tcgggtcatt ctccacgcgg acctggacgg gataacggag
    20941 gtacccggca tcccggaagt cgagggagaa actggggtaa taaatcccgg gagggacgtc
    21001 cgcctttatg gtgaaggtga actgcatccg gttcccggca ccgactgtgg tcgtggtgtc
    21061 ataggaactg gacaggatgg agatgtctgc atcatacatc gttgccctcc gtattgcgac
    21121 gctttccagc ccgttattga tgatctctac catgacgatc cctgtatcac cctcaaagaa
    21181 gacaccaggg ttgaggtcaa cgctcgagac cgtgacctgg gctgcaccct ccccggcctg
    21241 ctgggcgcct gcaagggaga tgaaggggga gaaggcgatc aatccgaaca ggaccaggag
    21301 aattcctgtt ttcattatgg acctcccagg aacggcattc cgacaaatgc agtatagagc
    21361 gtatagatcg caaaaatggc taccgggata agaaccacga ttgcggcatt ccgaacggtc
    21421 aggttcctgg catgcctgat gccgaaaatc cagaggtttg cactccatgc aaggaagatg
    21481 accgatatca ccgccgagat ccgggtaaac tcgcggaagg ccggatccgt caggatatca
    21541 ctcataaaac gctgcacagc agcgggatcc tggatgttcg agatgaccgg cacttccacc
    21601 aggggaatat aatagaggga gacaatggcg gagatgatca acccaatgat gaccgggaca
    21661 agaccatccc cagtgaattc aagggttctc ctgaacgtgc cgttccctgc aaaagccatg
    21721 gagaggagat aaaatatccc cgcaaagatg atccaccaga ggatcaggaa ggcaaaaaat
    21781 gcagagagtg cactgaaagc ccccatcatc gaggccattc cctctcctgc agcagcagag
    21841 aacagacctg aatagacccc ggacatcagg taagctgtca ctgcggagat gattgcccca
    21901 atcaccacga ttattccggg aattttcagg tcctcgggct ccttcatccg ctcactgaaa
    21961 aatgtgtcag ggcgaaagag gaggtatggt agcatccctt tcatatgaat cggagtaatt
    22021 tttgatgtga aagtctatca acattcccca gcggacacct gattattctg ggaattgagg
    22081 gggaaaacag gtttttcact caaaaacctt ttagaagatc ctcctcgctc ctttattagg
    22141 gaaggccgag tcgcggtggc cactaaagtg ggtcaatgtg ctgccattgg gcgcacagga
    22201 aaggcgcgtt tatcgtatgg tggggtgtgg gccggacacc cccactcttc atccgttcca
    22261 tcatttttaa accaagagaa gagcgaagag ccttaaaaga actcctttct ccagaaaaat
    22321 actatgccga tcacggatgc gataatcgag aacgcgatcg cgataaggaa ggcataaggg
    22381 ttatgctgga acggcaattc gacgttcatc ccgtagatgc tcgccacgag ggtcgggatc
    22441 atcaggatga tagtgacagt ggtgaggagc ttgatgatca cgttcaggtt gttcgagatg
    22501 atggaggcga aagcatccat tgtcccggtc aggatgctgg tatagatgtt tgccatctcg
    22561 atcgcctgct tgttctcaat caccacctcc tcgaagatgt cccggtcgtc atcatccatc
    22621 ttcatgcagc ccgaggtatt gaacttctcg agcatcaggg cattcgaccg gagggaggtg
    22681 atgaagaaca ccaggctctt ttccatgttg agcagccgga tcagctgttc atttcggagg
    22741 gctttgtgaa ggtcgttctc aatcgctgcg gtggtccggt tgatatcctt aaggtaccgg
    22801 aggaacatca aggaggtgcg gaggaagagc aggatcacaa accgagtccg gttcatggtc
    22861 gagaagttac ggaccttccc gttcaggaac tcctggacca cgtcaacctc ggtcagggtg
    22921 accgtgataa caagcccctg cgtgaggatg ataccgatcg gaagcgtggt gaaggggata
    22981 tcggcctctg tagcctcacg tcggggtgtc cggagcacga tcagggtatt tccttcctcc
    23041 cgttcgattc gggctctctc atcaacatcg agcggatcgg taaaaaaatc gggggggatg
    23101 ctgaagcgca agaccaggtc ctcgatctcg gcctggctgg gactgaccac atgtacccag
    23161 gcaccgctct cgaaggtggt gatggtctca agaccatttt tcggaccggt cttgtagatg
    23221 gtgatcatgc tcattccccc tggccgccgt ttcccctgag aacgggcatt gtgcctgatc
    23281 agggtatacc cggatcaagg tggcattcca tcccttatct ctccttcgca gtgacactgg
    23341 gatgatctcg agaagtgtgg atgcaataaa aagggcggtg atgccgtccc tgatcgtgtt
    23401 ggtactgccg gttgttatcc tttcctaaag ttgcatacca tgaaaaaggg acagtgacgg
    23461 tcaggatgct gctacgtgaa cgagcgatga caatgaaaaa aggatgagag atcaggtgag
    23521 gcttttaatg cggcgcaccg cttcctggag gtgtcccatg gaggtcgcat aggaaattct
    23581 ttgccatcct ggtgtattga atgcagaccc gggggtaact gccacgagcc ccttctcaag
    23641 ccacttccgt gcagttccga tatcatctcc accggtattc acataggcat agaatgcccc
    23701 ttcggcagga gcgacagtgt agcccatcag gagcagctcc tggacaaggt aatctctcct
    23761 tcggtgaaac tcctttcgca tcgtctcaac acaggactgg tcgcaggtga gggcggcaac
    23821 cccgccatac atggcaaatg tggtcggatg gcttattgag tgctgctgca ccttggacat
    23881 ctggttgatg atcgcctgcg gggcaacagc ccagccaagc ctccatccgg tcatggcata
    23941 tgccttggag aatccgttca cagtgatggt ccgctctgcc atatcccgga tcgagccaag
    24001 ggagatatgt tccctgccgt agatcagctt ttcgtaaatc tcatccgata gtgagaagag
    24061 gtcataatcc tcgcacatgt cagcgataag accaagcgag gaacggttca ggatcgcccc
    24121 tgatggattt gagggagagt tgatcacgat catcctcgtc tttccggtga tcttatcgag
    24181 aacagactca tcgacctgga acgtctttga attgagcagg tgatggacgg gttttccccc
    24241 ggccatccgt acgcatggtt cgtacgagac ccacgatgga tcaaggatta tcacctcatc
    24301 tccagggttc agcaccgcga ccatggcatc atagatggca tttttggcgc cgcaggtgac
    24361 gattacctgc ccgggggtgc aggttatctt gttttcctgc tccgtctttc gtgagatggc
    24421 tgagagcagc tccggaattc cattgcttgg agcataatgt gtctccccgc aggtgagtgc
    24481 ctggatacag gcctctttga tatgggccgg ggtatcgaag tccggctcac ctatcgagag
    24541 gctgatgaca tcgatcccct gccgctgcat ctgccgtgct ttatcagaaa tttcgagggt
    24601 tgcagaggga gcgatcccgg aaataatacc tgagagctct ctcatgccaa tctctggcac
    24661 atttttaccg cggattcaac ggcgcgtttc gcataatcga tacgctcgtt tgcctccaag
    24721 cgtgtcatac ccggcccgga gatgccgaga gtgaccggct tatcgaattc aagggagaga
    24781 tcgatgatct tccgagctgc gtgctggacg acgatctcgt catgctgggt tgcaccctcg
    24841 ataacgcagc cgatagtgac gaccgcatcg accactccct tcttcagcat ccttttcacg
    24901 gctaggggca tatcataggt cccggggacg tacatgcaat cgatcacttc agcgccaagg
    24961 aacgaggcat gctccctccc ctctacttcc atcatgtagg tgatatcccg gttgaattcc
    25021 gagataacga aaccgagttt cacgttcata tttttcctct ctctatatct cccactgata
    25081 attcataaac gaatgccacc tcacccacct gggcagctca tctccgtgca gggccggcat
    25141 cgggaaatcc ctgccgctgc cctgtccctg cttctttcgt cagttcctgg gggtagtagg
    25201 cgattttaag ggcattttta gcatgctcgc gggtacgctg ctcggcaagc catgcgagtt
    25261 cccgatcgtc cctggcttca tcttcatgca cgaagacctc gatgatgtgg gtgttggtca
    25321 tgagctggca ggtgatcagg ccggttgatg cctcgtgggc acacatccgg tccttctctt
    25381 ttcctcccgg cattccaagg gccatgacaa ggccgcagtg gcgctcctcg atcagctttt
    25441 tgcacgcaac agggagatcc ttgatcccgg gaacggtcac ccgttcaata gtgacactgg
    25501 catttctttt cagttcatca gcggctatcc cgcccatgtt caccctagca aaggttgtat
    25561 cggcaatccc gatcttcatc ccagtacctc agcggcagcc ctgaccccgt ttccggccag
    25621 ccggactccc tgcctggcaa gcgcatgctc gaccgccgag agcgtggcaa ggatctccgg
    25681 tgcaccggtt gcccccatcg ttcctatcct gaagatcttc cccttgagat ggtcctggcc
    25741 gcctgctatc tcgatcccca tcttcttgat tgctcctctg aacgcgctgt cctcgacccc
    25801 ctccgggagg cgggttgcgg tcaccgtgtt cgagtaggca tgatccccat cgagtttcgg
    25861 gaagagggcg agtccccatg cggaaactgc actcctgacc gcctgcgaca tcttcctgtg
    25921 gcgtgtaatc ctcgcggcaa ggccttcctc ttcgatgatc aggcatgctt cgcggagcgc
    25981 aagaaagagg gggacggccg gggtgtacgg ggtctccatc acctttgctg aggcgctctt
    26041 ccggtacgcg gcaaggtcca ggtaatacgg ccttctctct gagagccgtt cccatgcccg
    26101 ctcgctcacc gatatggccg agaggcctgc aggggctgca aggcatttct gggagccgac
    26161 aacggcgata tccgcccccc atgcatcggc atgcacctca tcccccccga tagaggtgat
    26221 cccgtccatc acgaagaggg cgtcatattt cctcgcgagc ctgccgatct tctcggccgg
    26281 atttttgata cccgccgagg tctcattgtg caccagcgtc accatgcctg ccccttcggc
    26341 aagctcgtgc tcgagcgcta ccaggtcaag cggggtgccc cattccgaac gtaccacttt
    26401 ggccgtgccg taccgctggg cgatcttgaa cattcgttcg ccgaatttgc cgttctcaag
    26461 gcaggcgatg gtcagcccgc tcccgaaatt ggctaccgcc gcctccatgg cagcggtgcc
    26521 ggagccgctg atcacaaaga gttcgttctt cgtaccaaag gtctctttca ggacacggac
    26581 acagtctgca tacactgccc cgaactctgc cccgcggtgg ttgatggcct gccgcaccat
    26641 tgccatccgg acccgttcag gcacggggac cgggccggga agcatcagga gaggttcttt
    26701 ttccatttta tcagtccata gtatttttat aatgacggga tataagtgtg gatggcgaat
    26761 caatggtcga agtagccatt ctcagcggat cggcctcaga tgcggccatt gtggaaaagg
    26821 ccgcggcagt cctggaagag cacggtatca gctatgaagt ccgggtcatc tctgctcacc
    26881 gggatcctga tgtgctcgat gaatatgtga aaaagagcga ttgcagagta tttatcgcaa
    26941 tcgcaggcct ctctgcagca ctccccgggg ttgtctcatc gaagactgaa agaccggtga
    27001 tcggtgttcc cgtcagcggg aaactgatgg gtctggatgc cctcctctca atcgtccaga
    27061 tgccgaaagg ggtgccagtt gcctgcgtcg ggatagatag cggagagaat gcagcactcc
    27121 tggcatgccg aatccttgag actcgaagag ggagggaaaa agtcttttga gtcgtcctgg
    27181 aatcctgctc atttcaaatc cgtttcccat gtcccggccc ctgttcattc ctgatcttga
    27241 tgaccctgtg tccttgccca ttccaggtcc ctgttcaatc ctaatccagg atcgttctca
    27301 ctaggagtcc ctgtgcaatc ccgatcctta ccaattcctg ttcactgtgc atgttcctgc
    27361 atccctgcct tcaccttgac aggctgctgg aggggattat ggcattcctg tccaaacaat
    27421 ccagaacaat ccagagtccc ccctcctcct gtgtacttat tcaaccagaa atacgaccgt
    27481 gacgtataat tgttcttcag cggtaacgaa gcctgattac cccggacaat ggccgaggca
    27541 tcgctgaaat gagagcagtg gggaaatgat tatttctttt ccttgaacat ctcctgcatc
    27601 attttgacgg cacagagatc cccgcacatc gagcaggtat ccagatcccc gtcccgtgca
    27661 tggattgccc gggccgcgtc aggaaagagg gcgagggaaa actgctcctt ccagtcaagc
    27721 cttctacggg catcggccat ctgccgttcc cgtggcattc tgaagccctc cttccccctg
    27781 atgagatccc cgatatgggc cgaaagtttt gctgcacggg tgccctcaac gatatctcca
    27841 accagcggca acgccaggtg ctcgctcggg gagaccatgc agaggaaatc tgctccgtgc
    27901 ctgcatgctt caactccacc gattgccgcg accatgtggt cataccctgg tgcaatatcg
    27961 gtcaccagcg gcccgagcag gtagaggggc gcctcattcg tcagttcctt gatcatccgg
    28021 acattgtacc cgatctggtc gaaggggatg tggccgggtc cctcgatcat ccgctggacc
    28081 cccgcgtcga gcgcccggtt tgccagccgg ccgaggttca ggtattccac gctcttcgca
    28141 tgccggtccg catcctggag gcatcccggg cgcattccat caccgaggct caccaccacc
    28201 tcgtgctcgg ccaggatctc aaggaggtaa tcgaactcgg agaagagagg gttctcctcg
    28261 ccggaacgct ccatccaggc cacatggaag gcacctccgc gggagaccac cggaaggata
    28321 cgcggatctg atttgagcga ggcgagggct tcccggttta ccccgcagtg gagcgtgagg
    28381 aagtccactc cctgcctgca atgctcccgg atcacggtga agaggagatc ggcactcagg
    28441 tccacggcac tccctgcccg cctgaccgct tcgtagacgg ggaccgtgcc aacaggaaca
    28501 tcgaacgaga ggatcatttt tcggatcgct tcgaggtccc ccccggtgga gaggtccatc
    28561 agggtgtcag cgccgtttgc tatggctgcc tcggccttct ctctctcgag ggccaggtca
    28621 caccgctctc ccgaagtccc gacattgaca ttgaccttca ccctgcatcc ttcaccgatg
    28681 gcactcaacc ggtgcgggcg cacgggattg gcagggatta cgacatgccc ccgggccacc
    28741 gcacgggcaa aatcccgtgg attctgccct tcctgtcgtg ctgccggctc aactatcata
    28801 ggaatcccgg caatacactc ctggatcagg gtacgcataa gacagatgta agatgcgcca
    28861 cttattagtt atgcatgcca gtcacctgga tccctggaag aggattcgtg gcgaatgcgt
    28921 acctgttcgg gaatattctc atcgatgccg gagtcctgcc ctctgcggtc gatccctaca
    28981 gggaggagat cgatacgatc gtgctcacgc actgccacta tgatcacacg gcacacgtga
    29041 aggagatagc ccacatgtgc gatgcaaagg tcgccatcca tcctctcgat gctcccgggc
    29101 tttttgagga tcaccagagc ctctcccttc acttcggcgc ccggtctccc ggcattgctg
    29161 cacagcagct cctgtcggaa ggagaccgga tcggtcccct gaaggtcatc cataccccgg
    29221 ggcataccca gggaagcatc tgcctgtatt cagaagagga acgattcctg atatcgggtg
    29281 acacggtgtt caccgacggt ggtttcggga ggtacgattt tccgggaggg gaccggaccg
    29341 cgcttgcaca gtcgatcggg aaacttgcat ccttaccggt agagggtcta tacccaggtc
    29401 acggcactcc tgtccgctat gaggggagac tccacatctc ggctgcgctg cagctcctga
    29461 agagcggata tgcataaagg tgtctactgc ctggtgttcc ggaacagatg ctgcacggtt
    29521 gaggtcggat cgctggggga ggtgacgttt ccctcgggat ggcatgtgta taccggttct
    29581 gcacagggtc ccgggggtct tgcccgggtt gcccgtcata tcagagtgaa acgggaaggg
    29641 atacgatcgc cccactggca tgtcgattat cttctcgtgc aaccaggatt caggctgagt
    29701 gctgttgcat gcgcaccaac aacagcgcag tccgatgaat gccggatctc cgggctcctt
    29761 gcccgtgacc cggtccccgg ctttggctgc agcgactgcc ggtgccggtc tcatctcgca
    29821 tattttaagt cgaatccgga aaaagaggta aaatccgcat ttttacacgc cggtcttgat
    29881 gtaaccatca caagactcaa tatatcctaa acccaagaag tcgagtatga cggttctcgg
    29941 catatcaggg agcatgcgga aagatggaaa taccgcactt ctcgtcaggg cagtgcttga
    30001 aacctgtgag aagtcaggat tgaagaccga atttatctcc cttgcaggga agaagattaa
    30061 tccctgcctc gggtgtgaga agtgcaagga gaaggattgg tgtgtcgtgc agaacgatga
    30121 ctggggagag attatgcaga aagtgattga tgccgaggtc ctcgttatcg gctccccaac
    30181 gtattacttt gacgtctgcg gccatctcaa aaatttcatc gacaggagtt actcgctcta
    30241 tcatcaccgg aaactggccg gcagaaaggc agtggcagta gcggtctgtg ctaataaggg
    30301 tggtgctcgt acaattgaga ccattgaagg atttttaaac tcccacgaat tctcctatct
    30361 cggctgggtg agaggaaaag gatacctcga gggggatatc ctgaaagaca cccgggcgct
    30421 caggagagct gaagaaattg gggagcgtat tgtcaagctc ctgaaaccac acgattgaaa
    30481 atatcgttcc ttccgctttt tcctggaaat atgcctgatc ataaaccaac tgcaaaggca
    30541 ttatcccagg aatatccccc cgttcagcta ttcctcctga ttccgcaccg ctatactgaa
    30601 tgatactcat gagacaggta acgatcttat catctggttc ctcaatcatg ctggattatc
    30661 agtcgacatg atcctccgtt cgcctatacc ccttcactga tgaccagcgc atggacctgt
    30721 tcgatcccgg tttctactcc atatgacaag gcgattcttt cgaagcatct ctcttccttc
    30781 cgggttgata ggaacgaaat cagatagttc cctcttcaaa tcaggaacag cccggcacca
    30841 ccaggaactc ccattgtcgc cgaacctgaa gtggcagatc gaagcatctg cgatcagttg
    30901 ctcgaactca tccttctttt caccttctgc cctgattcat cgcatcatgc cctgccggtt
    30961 cctcccttaa acggaaaagc tcctgcgaat agaaggaatc acttatgtca gacaaaccgg
    31021 tagagggtat ccctctcgcg cagcgttctc ccgatatcca aagcgatgcg tttcatctca
    31081 gcaggatcga ggtaatcggt gttcacggcg cccgcacctt tcgagatgct ctcctcatac
    31141 atcgtacccc ctaaatcatc tgcgccggcc ataaggccga tctgggccat cttcaccccg
    31201 gtcttcaccc aggagacctg gatgtgatcg aaattgtcaa aatacagccg ggacacggct
    31261 gtcatcagca gatcctcccg cccggttgcc ccgggtctag caagaccttt ccggaacagg
    31321 ggtgtgttca tgtgcacaaa ggagagcggg acaaactcgg tgaagccacc ggtctcgtcc
    31381 tggattgcgc ggaggatgga gaggtgacga acccggtctt cgacagtctc gcaatgaccg
    31441 tacatgatgg ttgccgtggt agggattccg agcaggtgcg cctccctgat gatccggatc
    31501 cattcatcag tcggaatctt acccgggcag atcaccttcc tcacatcgtc tacaaggatc
    31561 tctgcggccg tcccgcagag cgaaccgagg cctgctcctt tcatctgctg gaggacctcg
    31621 gaggtgctga tcccgctctt tcttgcggcg taagcaacct ccataggatt gctggcatgg
    31681 atatgaaccc cgggagcacc ctccctgagc gtggtgatga taccggtata cgattcggcg
    31741 tcaaaatccg ggtggagtcc gctcacggta catatttccg tgacattccg ctgacgggca
    31801 agtgccgctt tgttccggat ctcgtccctt gaagagagat agccacccgc ttcaccttct
    31861 tttttcgaaa atccacagaa tccacaggaa ttgatgcaat aattggtgac attgatattc
    31921 tggttcctga caaacgtgac aatgggtccg gtgaccttct cgcgtttctc atctgccgag
    31981 gctgctatct gccatatcct ccggtcattg acggaaaaga gcatgagggc gtcctcatca
    32041 ctcatccggt aacccgcaag aatgtcccgg agaagggtcc tgacacccct ttcatccctt
    32101 ttccccgtgt ttaccgtgct acttcttgac aaggctatca tggaacttct ggagccgccc
    32161 agatataaaa cagctgacca tgtcattaat cccccatcag tccaagagat ggtgaggatc
    32221 catgaagagt gaagagatcc agctggagaa ttcaatggga accgggtatg tcctccctct
    32281 tggaccagtc accctggtct gggtggttgc cagacggggc ctggtggggt gtggtgcatt
    32341 cgatgtggca gcactgggaa gattcggcta tcctgcagcc agggtgcggc cggcctgtgg
    32401 tgactcagtt gcgacgctcg aagaccttgt ctggggggtg atcaaggagg cgaatgtccc
    32461 tgcccagaag ctggggatat cgaacgggat gagcggaaag gaagcacttg atctcctctc
    32521 ttaggtgaaa tccaaggatt taacccgcct cgtgatcacc ttctagtata ccatgaaata
    32581 ctcccacctg ttcggcccgg tccattccag gcggctgggc cgatcactgg gtatcgatct
    32641 cgtcccagtc aagacctgca gcttcgattg cgtttactgt gaatgcgggc atactacccg
    32701 ggagacaaca gagcgccagg agttctttcc cctccgggaa gtgctggaag aactccgcac
    32761 ctatctctct tccgcacccg ttcttgattt tattacgttc tccggttccg gtgagccaac
    32821 cctctccact tcaatcgggg atgtcatctg tttcctcaaa gagtcctatc cccagtaccg
    32881 tgtggcggtc ctgaccaacg ggagtctttt ctggcgtttc gatgtgagaa acgatctcat
    32941 gcaagccgat ctcgtccttc ccacgctttc atcggtattt gaggagactt tttctaggat
    33001 ccagcggccg gcacctggca ttcacgtcgc agaggtaata gaggggctga ttcagttccg
    33061 cagggagtat gcaggggaga tctggcttga ggtctttatc atacccggga tcaatacgag
    33121 ccggcgggaa cttgaaggac ttcgtgtcgc gattgaacag attgctcctg acagggtcca
    33181 ggtcaacacc ctcgaccgcc ctggaaccga gcactgggtc cgcccggcct caccggcaga
    33241 actggagagg atccgtggtg cactgggaat ttccgggctg atcccagtag agccgatcag
    33301 gtatgaactc acagccggtg cacccatgac cacggaatgg gaagaggctg ttgccctggt
    33361 ccgagagctg atccgccgga ggccgtgtac acttgaggat attaccactg caaccggcct
    33421 ctcccgccgc gagatcctca aaatcctgcg ggagatacag atcagttcgg ggatccagga
    33481 gagccaggag gagcggggga tattcttctt ctgcccggaa tgaatctccg gctatgaaca
    33541 caccccaccc ggggaacagt cctattctcc tttacggaat tgcttccgcg gtattgtttt
    33601 ctccaagaag aattaccttt tttacacatc attcgcataa tttagatttt caccgaacta
    33661 atgtctaatg aaccaccgtt ccatttcata aaaaacggca tcatctggtg attgccatcc
    33721 tggtccattc caggatcatg atgacagggg caggcaggtc taaggctcag tcccgggaat
    33781 caattatgag atgcagtcct tttgcagaat gaaaaaaaga gtgagatcca cacaacccgc
    33841 tgaactcaaa ccgttcagga agtgaggtgc aatcctggtc tccattcaac cctgctcagc
    33901 ctctaccagg gaatagtcgg tccgcctctg tcgaagcggt ctaccaatat cccggaccat
    33961 gatctccatc atggcaggat ccagataatc cgaaccagca gcccccgcgt cgccggatac
    34021 atcatccgag aacatcgtcc ctgcaaggtc gtttgcccct gcaagaaggg cgagctggcc
    34081 gagttttacg ccgagtttac cccaggagac ctggatattt ctgaaattat cgaggaacaa
    34141 ccgtgctacc gcggtgagga ggagatcctc cctcccgctt gctcccggcc cggcgatgcc
    34201 gtcctggtag agccgggtgt tctgatagat ataagagagc gggacgagct cggtgaatcc
    34261 tcccgtctcg tcctggatct cccgcagaag ggcaaggtgc cttgccctgt cctcctcggt
    34321 ttcacaggtc ccgtacatga tggtggcagt ggtagggatc gccatccggt gagcttcttg
    34381 gataatgccg acccactcgg cggtggagac tttccgtggg cagatcacgg acctgacatg
    34441 gtcagccagg atctcggctg cagtcccctg cagggacgac aacccggcgg atctcatcct
    34501 ttcgagcacc tcgcgggtcg tgatgccgtc ctgccgtgcg gcatgcatca cctcatcagg
    34561 gctgaaggca tggatgtgaa catggggtgc tgccctgtgc acggtcttga ggatctcctc
    34621 ataggtcact gttgtaaagc ggggatggac tcctgagagc aggcagatct ccgtgacttt
    34681 gcgggaggtg gcaagagcca ccttcttttc gatcgtttcc gtgtcatcgc agaaagctcc
    34741 atcgtcgcct tcattccggc caaagccgca gaacccgcag aggttcttgc agatgttcgt
    34801 gaggtggata ttctgattcc ggacataggt caccactgtt ccggcccgct cctccctgag
    34861 tgcatccgca agtgagaata tgagaaagat gtctcggcct cgtaccttga ggagggctgc
    34921 agcctcctcc tcgctcaggc ggtggcctcc ttcgacatca cggagtatgg tactgatgtc
    34981 catgacgggc tctaatattc actttttctc ctgtttcttc ccagattccc cgcttttcct
    35041 ccgctggtag aattcccaca ccaccagcac gaggatcagg ataacggcga ccagtttgat
    35101 attaagaggg atgtatccga tgagggggat ggcaaaaaat gccttcccca cgatccattc
    35161 gggtttcacc ggctggatca caccgagttc tgccctggag aggtaggatc cctgatcact
    35221 ccggacatta ttatccccct tggtgagata accgctgttc tttgacacct gttcagagat
    35281 gacaatatac ccttcatcag gagtggcatt gcccttgtcc gggatgagga gcccgttctg
    35341 tatccttgca acaatcgtgc cgtcctcaag aatccaggcg ttatcatcga ttgtgaccgg
    35401 gagataacgg tcagggctgc cctgccccgg gtagaaatag tagtataccg ggatgctggt
    35461 acttgtctca gtggtatcca ttgcacggtg gataatggga tggatcccgc caccaaggcc
    35521 agggatgagc gaaccttctg ccccgtttgg ggagtaaatc agcacatcac catactcatc
    35581 gaatttcata tatccagtct ctctgccgga ctgccaggtc tgcagagccc cataccggtc
    35641 cttctccacc acgaatacca ggtccccgac gttcatatgc ggcaccatgc tctcggattc
    35701 gatggtcacc acggccggcc aggtaccgga agcaagatac aggacaaggg caatccctgc
    35761 aaccaccgcg acaatccaga tgatctcgcg ggcgaatgcg atccagggat gttcactggt
    35821 gcggagggtc ccgataaggc tcttttcctc tgcttttttt ttcggatcca ccatactata
    35881 acttacctct attcgcattg ctggctatta aggaatcgcg atggttgatc ctgagccgga
    35941 taccgtcaga ccttccctcc agggtcccga tccaaacaca gaaatcacga gatggcaata
    36001 ctattaacga acgtgggatg ctcgacacca ggacaatcgt tcaccgtttt ctcgagacca
    36061 agatgcaggt ccatcctgat gtggtgaggt accttgccga gcagggtgac cccggtctta
    36121 tcgagacaat catcgagagc atcccgaaag acaccattgt tgtctccgtg aaacatatcc
    36181 ctggaattat ccccgaccgg gatgggagca ggttttccgc cgatccccgt tgcgaggttg
    36241 tgaagggcaa caacggtgca tcggggtatg gagggaccgt caccgattac gtccagtatt
    36301 tcagggaccg gtacaacagg ctgggaagca tcgtccgctc ccggtgcact cctgtcccta
    36361 tcgaagggct gctcaggaac tcacgctacc gggaggagac ctgtacggta cttggccttg
    36421 tgatagagac acggagtaca accaatggtc accgtatggc agagctggaa gatccctcag
    36481 ggacgctccc ggtcctcttt cataagggac gccctgtctt ttccgatgga gagacgctga
    36541 tccccgatga agtgataggg gtgaggggga ccctgtcggg tgatggcagg ctcttttttg
    36601 ccgaccaagt cctccgtccc gatgtcccca ttaaccatgc cccattcacc agcagtgagc
    36661 cggggtcggc agtgctcatc tctgatatcc atgtcggtag cgataccttc ctcgagaatg
    36721 cgtggaaccg tttttctgac tggctcgatg atgcggaggt atcatatctc ctcatcgcag
    36781 gcgatctggt ggatgggata ggggtgtatc ccgggcagga gcacgaactc accatcgcca
    36841 atatctatga acaatatgat gccctgggcg ccatgctgag tagtctgcca tcaagaatgg
    36901 tgatcgtaat aagccccgga aaccatgatg tcgtgcgtgc tgcagagccg cagcctgcca
    36961 tacctcctga atttgtcggc agatttccaa aaaattgcat gttcgttgag aatccggcac
    37021 tggtcagtct ccagggtgta atggtccaga tgtatcatgg caggtcgatc gacgacctga
    37081 tcagcctggt ccccggcgca aaatatgaag atgcttctcc gatgattgcc gggatgctcc
    37141 agcggcggca tcttgcaccc acctacggca agaggacccc tatcgccccg ggacgggagg
    37201 atcacctagt gatcgacccg gtccctgaag tgctccatac cggccatatc cacatcatgg
    37261 ggatctcaga ctaccgtggc actctttgtg tgaatgccgg gacctggcag tcacagacct
    37321 cattccagaa acagatgaat atcaacccga ctcctgcaag ggcggttcag ctcgatctgc
    37381 agacactgaa gcccacggtc cttgatttca attgatgtca gtcgaggctg cgctctggta
    37441 aagagaaaaa tacacgggtc tgattgagac accagcataa aattagcgaa gcccttttca
    37501 catcagatga gtcgttcact ttttatcctc tcatagtaca ccagtataag tacaaaaaca
    37561 ccattttcac gagattcgga ggatagtatt tgatggatct gtttattctt gtcccaatct
    37621 gtgcctttat cggccttctc tttgctgcct actcctacat ggttctgaag cgtgaagaag
    37681 agggcagcga tctgatgaag aagatcgcag gtgcaatcag gcttggtgcg atggtctacc
    37741 tgaagcgcca gtacactgcg atccttgtct tcatgatagc gatggcaatt gtgctcgccc
    37801 ttgcaatcaa tccgttcacg gcagtctgtt tcattgttgg ggcttcactc tctgcccttg
    37861 ccggttacat cgggatgaaa gctgccacct atgcgaatgt ccggacgaca aacgctgcca
    37921 agaaggggat ggcaaacgca ttccgggtgt catactcgag cgggaccgtg atgggcctaa
    37981 tcgtcgtcgg actgggcttg ctcggtctct cagtgctctt ctatgccttc tcatcgttta
    38041 ccgcactgac gctggctgac accgtgaatg tcctcttcgg cttctcgctc ggagcaagct
    38101 cgatcgccct ttttgcccgt gtcgggggag gaatcttcac caaggcagcc gatgtcgggg
    38161 ctgacctggt gggcaaggtc gaggcgggca tcccggaaga cgacccgagg aaccccgcgg
    38221 tcatcgccga caatgtcggg gacaacgtgg gtgatatcgc cggaatgggt gccgacctgt
    38281 acgaatcgta tgtagggtcg gtgatcgcga cgatgggtct tgctgttgtc gcatccattg
    38341 ctgcgggatt cgtgaatgtc ccggtccaga acctcgtgct cctcccgatg ctcatcgctg
    38401 cgcttggcat catcgcgtcc atcatcggga cgttcttcgt gcggagctcg aagaacgaga
    38461 gcagtgccat ccacaaggcg ttcaatatcg ggaccctcac gagcctcgtc ctcacggtga
    38521 ttgcgacctg gtggctggtg gataccctca taggacctca gtatatcggg gtgttcattg
    38581 ctactgttgc agggctggtc gcaggattcc tgatcggcct cgtgacagaa tactatacct
    38641 cgtacgagcg gccccctacc cagcggattg tcaaggctgc ggagacggga gccgcaaccg
    38701 atatcattac cggcctggcg gtgggatttg aaagcaccat ctggccggta atcatcattg
    38761 tggctgctat cctcattgca gcaaaccagg cagggttgta tggtattgcc cttgccgggg
    38821 tcgggatgct cgcaaccctc gggattaccc tctcggtgga tgcctatggc cccgtcgctg
    38881 acaatgccgg tggtatcgcc gagatgtccc accaggagaa aggaatccgc gagatcactg
    38941 acacccttga ttcggtcggc aacaccactg cggccatcgg gaagggcttt gcaataggcg
    39001 gggctgccct gactgcactc gcactctttg cagtgtatac ccaggcggtc agccttcagg
    39061 tggtcgacat gctcgatccg attgtctttg tcggtatcct gattggggca gtgcttccgt
    39121 tcctcttctg ttcgttcgcc atgaccgcgg tcgggcgggc tgccggctac atcgtcgagg
    39181 aggtacgccg tcagttcaag cagatcccgg gcctgatgga ggggaaagcc gaaccggact
    39241 atgactcctg tatcactatt tccaccaacg ccgccctgaa gcagatgatt gctcccgggc
    39301 tgatggccat catcgccccg gtgcttgtcg ggctgacgct tggtaccgct gcccttgccg
    39361 gcctgctcgt cgggtccatc tcgactggat tcatcgttgc aatcatgatg gcaaacgcgg
    39421 gcggtgcctg ggacaacacc aagaaataca tcgaggcagg acacctcggc ggaaaaggat
    39481 cgtttgccca caaggctgca gtgaccggtg ataccgtcgg tgatccgttc aaggacacgg
    39541 ccggcccggc attgaacatc ctgctcaagc tgatgtcaat tgtctcgctc gtgttcgccc
    39601 ctatcttcat ctaatctttt ttcaagcctg tattctgctg aaacccgggt tcttccctca
    39661 aaggatcaaa gcaagttgtt gatatatccg gcggattgga aagggaaaaa atggtattcc
    39721 tataatcctg gccatctcat cctctctcag gccttctgca ggcagggctt ttacaatagg
    39781 tggaaaaata tttgatttaa cacctcgcga actcaacact catacggaag ggatgagagg
    39841 agagcggggg ttgtatgatc tgcagctatt gaagagtttc aatagctgat tagggacctg
    39901 tgagacgtag aaaagagcct aagcgttcct tttagatgta caatcaccag caatacctat
    39961 cgttgggtac acaggtaggg ggcgaggcgg cggctcaacc ccaagacctt gttgttcgat
    40021 aacagtcgac gaaggactac ttcgctttgt cacctatctc tttggtgagt tcctcaagtg
    40081 tctcatcagg ggtgacgacg cactctttca tgtttttcct gaaattgtac atcatcatca
    40141 ggaagatccc gatgctgatg atgaggatcg cccagaggaa gatatcctgc cgtgcactga
    40201 agaccgggag agaggcgacc aggtatccga agagcacgaa tccggtgacc cagaggatcc
    40261 ccccgatgac attgtagaag agaaacgcgg ggtaccgcat cttgataatc ccggcgacaa
    40321 agggtgcaaa cgaccggata ccaggtacaa atcgggccat aacgatcgtt ttcttgccgt
    40381 atttcttgta atactgcctg gtcatctcga catgcctttt gtcgacgagg cacttttcac
    40441 ggtggatgag ccagttgcca aaggaggtac ctatcttgta attgaccgaa tcaccaagga
    40501 tggcggcagc ggagaggatg atgatgacca cgaaaacat
//
LOCUS       DAOOCO010000002        36878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_844_length_36878_cov_5.063330, whole genome shotgun sequence.
ACCESSION   DAOOCO010000002 DAOOCO010000000
VERSION     DAOOCO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 36878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36878
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_844_length_36878_cov_5.063330"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PK154_00200"
     CDS             <1..771
                     /locus_tag="PK154_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPW09513.1"
                     /translation="VYVLDDSMATIGELTHIAEQETIYSTRFIGDRLYMVTFKRIDPF
                     FVIDLAKPENPKILGKLKIPGYSDYLHPYDATHIIGIGKETTTNDWGGVSTSGIKLAL
                     FDVSDVTSPKQLDKVQIGDAGSDSAALSDHHAFLFDKGKNLLVIPLRAVSNAPLVKGD
                     YSTSQPQIWYGAYVFGLTPETGFTLRGTVQHGSGDSGYYYYGSSTSEVKRSLYIGDIL
                     YTMSSKQIKANPLDDLNTTITTIPLPNGGDILYPPLVK"
     gene            1218..3350
                     /locus_tag="PK154_00205"
     CDS             1218..3350
                     /locus_tag="PK154_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPW09514.1"
                     /translation="MDLKREKTRLSVRTKIFLVFLILSVTALLLTGFLAFVQMGNVSR
                     FAVDRSTDLASSIMNDSSAALEQDAEDALLRLATDQAHISDILFRQVGSETSSMATYA
                     LRIQQDPSMIRERHFYLQDDPPIDHLSTSVLFLSPGVESHIPVEERNAIGMMDSIFIP
                     VYSSDPNLAAVYMGTSSGMSVIYPWFTGMDAAFDPRTREWFRQAEQTGNLTWSKPYVD
                     LLGHGLMITCSKPVADPEMGWFWVVGADITIETINQNIIGTQVGELGYAMLIDSEGNV
                     ISRPGLSSGDMRWDESFETENLVISENPGLRAVAKDMISGNTGVAKVQYENGERFIAY
                     APVESVNWSVAVVMPVDEVVAPAEATRESILAASGETASHITGQQDQMRTIFSGAFFV
                     LLAAVGILTLLFTRHLTRPMEELRKGSEAIGAGNLDYAVNVQSGDEFEDLAASFNHMA
                     ADLKDHIENLKKTTAEKERIEKELEIAKVIQQSFLPETAPRLPGMELEGFNLPAAEVG
                     GDFYDFIALDDEQWGLVIADVSGKGVPAALFMALSRTLVRASTSHYQDPAESIREANL
                     NIFLDSKTSMFVTLFYAVLDASRKVLTYVNAGHNPPMLFSSGTSGVTLLEAQGIALGV
                     IDTIDLESVTVPLHPGDVVVLYTDGVTEATNDRDEEYGVTRLRDIVMASRDLPAREII
                     TTIVQDVIRFTGARPQFDDITLMILKVS"
     gene            complement(3564..3821)
                     /locus_tag="PK154_00210"
     CDS             complement(3564..3821)
                     /locus_tag="PK154_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09515.1"
                     /translation="MDIAGSRYFIVPDDVPSLLFHKPVDVVDPSGEREGTAWLSPIHG
                     PEKHELTTLIEQHLYVIGFRDLGRILAGEAIAVPVREYHPI"
     gene            4154..5134
                     /locus_tag="PK154_00215"
     CDS             4154..5134
                     /locus_tag="PK154_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HPW09516.1"
                     /translation="MERRSAFLLLVCLAGAFILCSGCTQPTDSLPNATPSSTLTMVTS
                     VDTPLLPPPTATTVTPVDTPIIPSSADTPSLTPTTLIPGDTTSCTKDYDCVPAQCCHP
                     TGCVNIRNQPDCSTIACTQVCQGPIDCGAGHCGYVNGTCSIIPGPASTPAGQATLKIA
                     VKDAPKIKDGGTITHLWLNISEVNVHRAVANQSINESNEEMTVIESEDPDTAGWITVV
                     NDTTMVDLMEFVNVSREIGQNTVDPGRYTQIRLKIDSGTITVDGINHDLSVPSGVLKL
                     NRGFDLDPEETLTLTLDFNVDKSVIRTGSDQYKLKPVIAVLSDEQQARGS"
     gene            5142..5549
                     /locus_tag="PK154_00220"
     CDS             5142..5549
                     /locus_tag="PK154_00220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPW09517.1"
                     /translation="MGQRDCPFCRVDLDEVIIDNDLVFARFDRFPVSRGHLLIIPKRH
                     FADYFEASREEKMAILEVIDQAKRFLHIRFHPDGYNIGVNIGEAAGQTVMHLHVHVIP
                     RYTGDVPDPRGGIRGVIAGRQHSGNLVNDQGEP"
     gene            5670..5801
                     /locus_tag="PK154_00225"
     CDS             5670..5801
                     /locus_tag="PK154_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09518.1"
                     /translation="MGFFSWLKDLVAVPVHDEPEKKRRKREDEEEEEIEELVAIDII"
     gene            complement(6053..6586)
                     /locus_tag="PK154_00230"
     CDS             complement(6053..6586)
                     /locus_tag="PK154_00230"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HPW09519.1"
                     /translation="MVEFGPVQMVAIGFPDPHKLKGDLLKELLALSEKKYIRLIGLLG
                     VVKDANGLIESVQITELSDADRKRLGAAVGALIGLGAARGKGAVAGAKAGAAAMSKEF
                     GLNQKQIREIARDIPNGSAAGFLLIEHLWAKNLKEIALAQDGIMLANGFITPDSLMAL
                     GSELAEGVKAAGKLTQA"
     gene            6795..7988
                     /locus_tag="PK154_00235"
     CDS             6795..7988
                     /locus_tag="PK154_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW09520.1"
                     /translation="MAIVVGSGAGGATVAKELAAAGHRVLIIEKGPAIDDIDAHRYYK
                     NVDAGVKLMRTCCLGGTTMVSAGNALRCLEEELLGFGIDLSAEFAEAERELGVCELPD
                     ELIGEGTFRLIEAASRLGFPVQKMPKFIDPGECCRDGRCSLGCPVQARWNAVRFVERA
                     LMLGAALVTGQAVTGILTHGSRVTGVRCGSREYHDDLVVIAAGAMETPRLLAGIGIPT
                     SPLFCDTFASIGGVCPGIGFHQDVPMGAYMPHSSSLILTHYARQFVTALKAAGHDAGE
                     GDILGMMVKIRDEASGSVGETIEKGVTEPDSRQLACGASIAGAILTEAGVDPATFVSL
                     PLRGSHPGGTARIGFSVDTRLMTRIDGLYVVDASVLPAAPGAPPILTIIALAKYAAKR
                     MEGTE"
     gene            complement(8286..8906)
                     /locus_tag="PK154_00240"
     CDS             complement(8286..8906)
                     /locus_tag="PK154_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09521.1"
                     /translation="MKRTVPGSRTKPGFRKISGVWALLVIVLSLLMILPAGGQITVVP
                     PEVTITDLETYHIGDTIILSGTTNLAPGNDLLVEVLSQSFGPTQEKAGNTFFGVSGTT
                     LVRKGPGEKNTWSFSFDTATFGPDTYLVTVTGVTVPKAVATSSFSLIASTLTQTPLQT
                     TPPTMLPTTIPTESTPPPTPTGLSVIPLVGAIMLTLAGFGIVRRKG"
     gene            9176..10492
                     /locus_tag="PK154_00245"
     CDS             9176..10492
                     /locus_tag="PK154_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPW09522.1"
                     /translation="MNQLAISLLLLSSLVIAALSLAGCTSPGTLPEELPPEGITPDTT
                     DPPGTPPGSPQSIVAANNLFALDLYSRLAGDPKNSGENLFFSPFSISSALAITYEGAR
                     GDTASEIREVFYFPADSEGMRAGYQAIIGGINNGSSAYQLRTANALWAEQTYPFLPGY
                     ISVADEVYGARATNLDFITKPDDSRVTINQWVEEQTNDKIRDLLPAGSINSLTRLVIT
                     NAVYFKGTWEKQFDEKETSIQDFRTSDGTILPVAMMQRTDTNATYGYMETGDLQVLLM
                     PYESDSGKHLSMMILLPKDGDLADLKGSLSAKWLKGLQQNLTTQRVMVYFPKFTMETK
                     YTLPATLSAMGMPTAFTADADLSGMDGTRNLFIGDVIHQAFVDVNEEGTEAAAATAVV
                     IQRAMAPAGPLPVPVFRADHPFLFLIQDDETGLILFMGRVEHPESS"
     gene            10682..11569
                     /locus_tag="PK154_00250"
     CDS             10682..11569
                     /locus_tag="PK154_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPW09523.1"
                     /translation="MYNAIKHRTFEILEQTRERDLTSQYFQIFIIILIVLNVIAVVLE
                     SVQVLLLQYYRFFLAFEIFSITIFSIEYLLRLWVCTESQEYHRPVIGRIRYALTPLAI
                     VDLLAIVPFFLPFLIPVDLRFLRVLRIMRIFRALKLIRYSAASSFMARALRREKYVIF
                     VSLLILAVLFVLASTLMYYAEYDAQPEAFASIPHAMWWAVVTLTTVGYGDIYPVTVQG
                     KIIGAVVAILGIGFFALPAGIIAAGFISEVRNSGCREDTTPSDARLTSLERLADLKAN
                     GYLTEGEFQQEKQKILEEK"
     gene            11708..13618
                     /locus_tag="PK154_00255"
     CDS             11708..13618
                     /locus_tag="PK154_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPW09524.1"
                     /translation="MLLFVGLMLLICGCTSLPPGDAGIPIESLTIYTEQNPPYNYEEN
                     GTLQGLSIDLFEAVTEQMGRPVSRDQIQLVPWSEGYQAALHGNKTMIFAIARLPARET
                     SFKWAGPIYPYTTVLFARPDSGIAITSTEELEGYRIGVIEDDAAVQQLLDAGVNQSQL
                     VLETNVSTLVTELQEGGIDLWAYSQTSGRYFARQLTGNAYSFTIVYTFPDIPIYFGFS
                     RDVPDATVQSFQQALDALKADKDVRGISTYDAIIGRHVPLVGLSHLQYLTEEWAPYNF
                     EENGTAGGIGVGILEAVFADIGVNLSREDVQIVPLAEGFREVQNGSSVLFSIVRSSER
                     DPLYQWVGPFTRGSFVIYAPMNRNITIASPDDLNRFRIGAVEGTIENTLLTDRGVNTS
                     RIINAPAPEDLLRMLEAGEIDLWATGDLAGRHQMMKTAEDPTTYEIVYTLSTNDFYFV
                     FSKDVPDTLVNAFGQALENVRLQRDAQGVSEYERVIYHYLGASCARQTFTDAEVIALV
                     NITAADIGRNTPDTFRRINAGEAPYKSPEHPDLYVFIYDTNVTLVASGDNIRQVGVNY
                     HGKTDVTGRPFRDEIVTAAIADGTGWVEYVYSNPLQTGVFYKNTYFRRAVGSDGKIYI
                     ISAGNYQSCEEV"
     gene            complement(13716..14501)
                     /locus_tag="PK154_00260"
     CDS             complement(13716..14501)
                     /locus_tag="PK154_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618527.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPW09525.1"
                     /translation="MKTILYYFTGTGNSLAVAEGLCRRLPDCELVPVASVARDPGRIA
                     PEGDRVGIIAPVYFSGLPAVVADFALRLDLARVPYSFAVLTMGGSGGTPALHQLERIL
                     TQGPGKRGLDAGFTVKMPGNYVLMYNPPNDATIGKILGKADRRIEEIAGMVEQGVQKR
                     PRMPVFGSLVQHLIYPKFIMGVHDADRKFSVDDRCTSCGICAKVCPVENIRLEEGRPV
                     WLHHCEQCMACIQLCPPLAIQAGKKTEQRQRYHHPNLRIKRPA"
     gene            complement(14598..15398)
                     /gene="trpA"
                     /locus_tag="PK154_00265"
     CDS             complement(14598..15398)
                     /gene="trpA"
                     /locus_tag="PK154_00265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPW09526.1"
                     /translation="MNSRIDGAFRLREGPLIVPCVVAGDPSFAESLQISRELVDAGAD
                     MLEIVMPFSDPVADGPVIQQAGSRARTAGMTTDRLFDLVREVRAETGIPLLIMTYANI
                     VIRRGIRKFYRDAADAGADGVVVADVPLEEADPFCAAARMAGIDPILFVSQTTSPERR
                     EQILAKAGGFIYLVAALGVTGTREDLSPQSIDLLKVVKERTSLPVVPGFGISTPEHVR
                     RYTHAGADGVIVGSALIRMIGDRGSSGSGPGNRIGDLVRAMKLAARGG"
     gene            complement(15395..16567)
                     /gene="trpB"
                     /locus_tag="PK154_00270"
     CDS             complement(15395..16567)
                     /gene="trpB"
                     /locus_tag="PK154_00270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPW09527.1"
                     /translation="MRSTSRFGEYGGQFVPESLMYALNELEEAYRTIIPSDEFREEMK
                     YYLTEYAGRPTPLTFCRNLSADLNCRIYLKREDLLHSGAHKLNNALGQALLAKCMGKQ
                     RIIAETGAGQHGVATAIAGAVLGLPVEVYMGEEDIRRQSLNVARMHMLGAKVSAVASG
                     SRTLKDAINEALRDWVTNVQDTHYLIGSVVGPHPFPSLVREFQTVIGNEIREQVMAKE
                     GRLPDAMVACVGGGSNAIGMFAPFLDDPVELWGAEAGGEGLAGRHGATLCGGTPGVLH
                     GSYTYLIQDAFGQILESHSISAGLDYPGVGPEHSFLRDTRRVIYRPVTDAEAVDSFLY
                     LSRTEGIIPALESAHAVGLARQIAGGRGPDDILIINLSGRGDKDVAQVAGLMGGNP"
     gene            complement(16564..17094)
                     /locus_tag="PK154_00275"
     CDS             complement(16564..17094)
                     /locus_tag="PK154_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPW09528.1"
                     /translation="MAQEAGADMIGVIAFSSSPRCIEPDMIREIFDAVPTLFQCCVSH
                     GPSPREMETLCDLAPDAIQVPPAIPIPASCTAMVFRSIRPGDRIPDDADVLVIDASHG
                     SGRQYDSAYALSVVQSARVPVFLAGGLTPDNVRAAIRTVHPAGVDVATGVEYAPGRKA
                     REKVRAFIQRAKEVRE"
     gene            complement(17130..17891)
                     /locus_tag="PK154_00280"
     CDS             complement(17130..17891)
                     /locus_tag="PK154_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HPW09529.1"
                     /translation="MMLEQVLRATAGRVAGLPRAEEYPLPPPSSSRSLNEAIHQKKPG
                     NRIIAEIKYASPSAGTIRRGGDPAGFARDFARAGATALSVLTEPYYFQGDAAFLPAIR
                     PHVDLPLLRKDFIIDERQLAESRALAADAVLLIAGVLGDRLGDMVDLAFAYGLEPLVE
                     VHAAHEVQEVLSTGTRLAGINNRDLRTFRINLGTTMKLSPLLREGGITVVSESGILWP
                     YDIRILRPSADAFLVGSTIMRAQNPGQRLEGLIRG"
     gene            complement(17888..18904)
                     /gene="trpD"
                     /locus_tag="PK154_00285"
     CDS             complement(17888..18904)
                     /gene="trpD"
                     /locus_tag="PK154_00285"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPW09530.1"
                     /translation="MMQAILARLADGEPLESDQAAAVMEMIATGQATGAQIASLITAL
                     HIKGETAEEITGFVQVLLRHALTIKPQVRGRFVDTCGTGGDGSGTFNISTAAAFVAAG
                     AGIPVVKHGNRSVSSSCGSADVLEALGVRIDVPPERVCSIVEETGIGFLFAPAFHPAL
                     RHAAAVRKELGFSSVFNLLGPLLNPAGAPARLCGVYSPALVDRFSHALLALGTERAMV
                     VHGQGLDEIAVSGPTLVAEIDQGAIHQYTVTPEAFGIPRSPHSALAGSSPKFNAAIIQ
                     KILSGVPGPAHDVVVMNAGAAIYLGDGAKDLGHGIEKAEDSITSGAAAGRLAALIRAS
                     RGIP"
     gene            complement(18901..19482)
                     /locus_tag="PK154_00290"
     CDS             complement(18901..19482)
                     /locus_tag="PK154_00290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPW09531.1"
                     /translation="MRVAIIDCYDSFTYNLYQLVGQLGSVPLPIPCDAPLEEVRDQDP
                     ERIILSPGPGTPDESGICRDVIREYAGSIPILGVCLGHQTIVQTFGGRISRMLVPVHG
                     KTSSIVHTGEGIFSGIPSPFTATRYHSLMVDPASLPPGFQVRAVSSDDQCIMAVTHPD
                     LGVTGVQFHPESIMTPTGKLLVKNFLVGSCGGA"
     gene            complement(19479..21020)
                     /locus_tag="PK154_00295"
     CDS             complement(19479..21020)
                     /locus_tag="PK154_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPW09532.1"
                     /translation="MYLFVHEYQVRDAAGLGNAVPRTAVNGHQPEQYPPFQVIPLKRI
                     IPLPGVDPFTLFCALKITKGYILESMEGVPRRAVRSIIGMEPEFEIVLGESPAISGTY
                     PGGSLFPPLLGDDPVSQLRSLSSRFSYTPGGEGDFDGGFVGYCSYDMVTSLSGGHVQA
                     GRDDIPLARFMCSTRGIVYNHVQGSCLLFEDILLPHGYEGDEAIRLAQQRLDALEKRI
                     QDIPIPAGTGTCSPAMGALPDPGQEDQVGYLEMVRKAKGHIMAGDIFQVVLSRKITVP
                     FNGNPLDIYSRIRSINPSPYLYYLNFGDEAIVGSSPEMLVKVQRGVVQTVPIAGTRPR
                     GKDPEEDSRLAKELLADEKERAEHLMLVDLARNDIGRVSTFGSVTVPEFMEIERFSHV
                     QHIVSRVCGIVARGKDRFDALASCFPAGTVSGAPKIRAMQIIADLEASPRGLYAGAVG
                     YCGFDDLLEFAIAIRTVRVKGGRAECSTGAGIVADSIPDREFEETVQKARAMVSAVGE
                     SGGGP"
     gene            21221..22174
                     /locus_tag="PK154_00300"
     CDS             21221..22174
                     /locus_tag="PK154_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPW09533.1"
                     /translation="MAKKTKGTNTRNGKNRALLIGIFIVIFLIMAGIFSSFSKVNDNT
                     DTASWSLDESSLLSVSGRTLPAITVSPLEETVNCTLEKISYQSDDTMVYGLLRLPREV
                     QIPPVVIVLPAATVTKEGDSAMADALCSFGYASLTLDERGNGGETQGVSPMDLQTGYT
                     AYRNREMPAQYAQIYDVLLAYDYLQTRKDLDGDAIAVLGESMGGRFAIITAGIEPGIK
                     GAFVVSSGPYGVDAGNNPDVQRFITSIEPESYLLKLPPRPLVMFHFTDDPIIPLAEGK
                     ALYNKALSPKAWHQYNGTVHGVYSETYAEDLHAELKEIFGR"
     gene            complement(22304..22558)
                     /locus_tag="PK154_00305"
     CDS             complement(22304..22558)
                     /locus_tag="PK154_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09534.1"
                     /translation="MDLSFYLQPVSIVIEAIICIIAIAIGILKKKIYGWFIALTFGIY
                     VVYDIIAFMDISLLPSVVAIIFLVATLSMLYVVWILYNEH"
     gene            complement(22656..23369)
                     /locus_tag="PK154_00310"
     CDS             complement(22656..23369)
                     /locus_tag="PK154_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPW09535.1"
                     /translation="MELGISSYCLIGRPLAETLDTLSWITDLIEVMDEGPHLITDPAV
                     FESYSQDFVIHAPYHGMNIASLFESIRRASVEVMIDCFAIAAEIGAPVVMHPGYHAWN
                     TEKEAADRQFEKSLHQLQESAADHSLMFWFENMGELNYFHFRTPDDIPSLGDTGFCLD
                     CGHANLNHCLPAFLETDFSHMHIHDNDGWKDSHSAIGEGTIDFRPVMAALERTAATAV
                     LEVKDLEGVLVSKRLLEGL"
     gene            23643..24221
                     /locus_tag="PK154_00315"
     CDS             23643..24221
                     /locus_tag="PK154_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09536.1"
                     /translation="MTVRVLTTILLLLFLAAAAGIVAAEEETTYYCYDYSTQSWRVCT
                     PEEAIQAMDALEKEIPKELPHEEWEAVINSNQPYWQYYLDPPTGTPTPTPTPTPTPTP
                     TPTPRPTLSPSLEQSFFTPWFSNAPGVIPTSAPMKYSGRITIPTKYPITSTPTPVPTS
                     TISPETTISKDPGNSFFSPMFPVRNFSLFKWF"
     gene            24276..24398
                     /locus_tag="PK154_00320"
     CDS             24276..24398
                     /locus_tag="PK154_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09537.1"
                     /translation="MYPGHPVATDLDLLFMKALTMNRKGGILPSLSQITAIDEE"
     gene            complement(24406..25140)
                     /locus_tag="PK154_00325"
     CDS             complement(24406..25140)
                     /locus_tag="PK154_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW09538.1"
                     /translation="MRIVAFNGSPRAEQSNTHVMVASFLAGAEDAGADTENIFLSNYS
                     IRHCLGCFGCWLKTPGTCVQSDDMEELIKKYRSADIVIFATPLYIDNVSGIMKNFMDR
                     LIPMGDPHFEKDPHGEVRHVSGEKKNQKFVMMANSGFPEQTHFQVLRLLAQRLARNFD
                     TMLAGEIYRGGGSLLQDEELKPWIDAYKMQLRKAGWELVKEGKITPETTFRLELPLIP
                     APEYVDMFINNANAYMDRMIEINRKG"
     gene            25379..26296
                     /locus_tag="PK154_00330"
     CDS             25379..26296
                     /locus_tag="PK154_00330"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPW09539.1"
                     /translation="MKKRQILISGLIAIFLFIVSATAAECSLVGTWGSIDGSYIYDLD
                     ENGGGQIIAEGVIQSCNWTEESKVVTITWSYGPVPGERYIDTVTLLDECDSFAGTNNY
                     GAPIFAARIVSPPFACSDEFSLQQNSALVVERPGVLINDYQPNQENLTAVLLSSPLNG
                     DLVFNDDGSFTYTPSPEWWGTDTFTYLVKNETEVGTSAQVRLVVTKPENPATYTITTS
                     RLGAGEISPSGIITLQPGENQMFLVLGEKGSRLTSFTIDSVTIDVVQDPPSPSTENQG
                     VVDIQSLVVTFENIMSEHTLHAVFTTPTI"
     gene            26795..27241
                     /locus_tag="PK154_00335"
     CDS             26795..27241
                     /locus_tag="PK154_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09540.1"
                     /translation="MRSMELGDEEDQRLEEIWITILRRLTRTISRTPSGIPEYHPGPP
                     KTPRESFRPLPHLAGVYASSDLAATIAGDLIGRDLPVQVERHETPPPYSQVIGDHTGT
                     LVHGHTGPVWVVWTNYHNNRCETMQGPAGKDMIPPGMRGHQTRKIP"
     gene            27238..28464
                     /locus_tag="PK154_00340"
     CDS             27238..28464
                     /locus_tag="PK154_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPW09541.1"
                     /translation="MKGMTHGFADALRLTLEHITLLPTETVSLLDSVGRIAAEDLCAL
                     IDSPSMDSSRKDGYAVRSGEVAGATPDHPVRLRLVGSIAAGGANNIGVIPGTTVRVLT
                     GACIPDGADAVVSEEFATKESHEVLIENPAAPKNILPRGSDVSLGQCLLSKGSSITPV
                     MAGLLAVAGHSRIPVYRSPAIGIVGTGDEIIEPGEPLPEGKIYASNIITLAGLCRRYG
                     GSSTLALVPDDPDTIFRTLHRLSLETDALITSGGAWTGDHDMVASVLGSLGWEQVFHR
                     IRIGPGKGVGFGMLGGKPVFVLPGGPPSNLMAFLQIALPGLQALSGDAHPGLPRIRAR
                     LGSDLVGGDRDWTDFFFGTLSLEGEMVVFYPMMKRSRLSAVAGATAIGAIPEGEDSLH
                     EGEVIGVQLIGHGMEP"
     gene            complement(28716..32684)
                     /locus_tag="PK154_00345"
     CDS             complement(28716..32684)
                     /locus_tag="PK154_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW09542.1"
                     /translation="MPGLSSFGPLKVTAWRSGSRVPVATEFAIGEIRIPAPAEPFIVL
                     VPSTISSLQQMWDALRMPSRNNLLVTGESGSGKTTLVRYLGEVYRQALLVMAAGLHDP
                     HAAAHAAERAASFQTRILTFHENLRRSDITERRHYGETGEEKTGWTMSDVMDGMVAGD
                     WNVLSEINRTGEDVQAEFNEPLENKAKSLHQRVIRGHPDSRFIATVNPQKSEGRGIYE
                     GKVMSGEFVNRFTNKVHLRFLPPDEEFEVLKDYGPGVDDSILVRLISVANDIRRDYTE
                     EHGVVPFPVTTRGLIRVVRHLARFPEDGFLLRSLFWRKAYWLDDRIHPPIARKLVLDL
                     LDLHDIVDAGDRRREHSRVVREEGGNAPLLRIGSVAYPLGQGGPYVPDTEIEEVQQNL
                     ADLEWILKDIALAENILLIGEAGVGKNKIESYLAHLLNYNLLVIGMSGETRVSDLLTY
                     RSFGEDEAGKTGDTATLGLRALTDPDHGWIIVLDEANKAQPGVLVSFNDLLQDRSVRL
                     PGGREVPVRASICVNINPNRPPYEVNDFSFEFMDRFSIHTIVHLPPGQAVEVLKRKYP
                     GADPDFVSDVVQGYYTLHPLYSSGMLFEPVTMRNEEAAIERGLQYASRAVNLVDLLCS
                     CYGPRDGRELHAVQAALSAAGYDRNVLAASAALERFRTSWDQEKGDEEKGIALAETYQ
                     AIGKPLAAIRVLREMIPHIPAGRDWVCHLRIAEILLACGRDANACDEIKAGFSPGGLL
                     LMDHGYEYRVLDMEVRVTQGRHAFSAEAVNHRTGYPALITNLPSSPAPSLHRNAGEYV
                     LVASDQMHTLAIYEMAQSETKIPASPSLAMIVREMGQACGSCQEFVRTLCRLPSPPWT
                     SPSYREGEVSREHPAGFRLVATPDGWAIHIPGEGVVHWKGGICAWSGREGGAQIPCAG
                     DPFRGLAYHEGTLFLILEDTCAAARGIRGAGFTSMAPGSYQSAILAPLIRTAYPHLEE
                     LFVYARPRSTVRLGFGDILYTLMPGKGIQIGFVVEVDGFGPVIWDLSPSEGDQDPLAE
                     QLVIPFSLLDVCEERRRQVLLPETQEHLQALRRYAASVFLSTAKPRDMIAESKMSTIL
                     AEFRECAEPIGRGTQLSRGDILVCDSNGRDHLRSVFIVRFLQEGGSSGGLKGTPPIVR
                     GFWLGQPNPVATLELASLPGRFGRLKVIDDDRYDRILSHLVASFQESGHPWQYARTGQ
                     AHDPLVHLEISNISLEYRPMERLFSFAVGRKGDGTGLVINQAGYPAGTTVLHAGDGRE
                     VRWQGLPISDIRYYKEQDILVVSLENDFLSAMNGILMESGVGSSGMEIRFIPWSPAGE
                     KI"
     gene            complement(32672..36262)
                     /locus_tag="PK154_00350"
     CDS             complement(32672..36262)
                     /locus_tag="PK154_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPW09543.1"
                     /translation="MEDHVHHLFRLLRMLLGSCAEHDPITDAFVTPANRDEYRTALEG
                     IRDCTATIRSTGITRDQLTSAIRERERHSPLTDGTIGDPRDLAPLILAQFTEIQEAQV
                     DFSCRVWGRTVSPRDPEPVVLPLRSTITPLSLEDLLSPGMTRMQRIPAALLLETAGEI
                     PSPGSPPLIADDGRILIPHADDPARFSGHLYTALFISLVHAGMIPEPVFSEFIRQAAA
                     DTGVIQNGGITAFTRIFGGDAFELFEAGEELFIREGMVPDIRELFAVILAREELEKDL
                     SFGRVEGYAGHLFAGMIQGASGDDQESGISLLIECARTLAGRVSGRAEPGDGLLKNLD
                     RTMQEQEQLLRAVSGDYQWRAVPILSTVPAFSPTLDLWPERHLILYNPDELAFRSGDA
                     TRALLMEAWYREWFGIQPHGHDLPDDPWFLRLVRVIGLQRAVWKGSALHPGVIRWIDR
                     FCQEEYSLVNRCADRGRIRRLPLPDQFLEGVLFEGRTGQPASHIEGDAVRHALAATAI
                     ARTDAVAAPDETYLQIIAEQVWPFLAHLCQIPSKGTTGGCVPHSSQKSPSYAPHGSVD
                     RPDTIVPSPHQPSHTATGRALAGNGTSMIGDGDGILTPGGSPFSETRPGRMSGRGRPS
                     LGKVPGARGSGEHGSGIPATADALVEACGRSEDLLDNLVGAGEQGVEAGADNIIPDQG
                     ETISDLSGLARQIDSLAETLEQQLLDMQEDANSIEGSPAGMMGEELDGLLKTSRDIRK
                     AAREYRDAVEAMEFLKEASASNSALRERRTGMTKEAFRDLQKAGVEFQRRCAASASAF
                     DEGGLKIRLTEAPGGPGIQPPTTSRGRMIPSFVPDTSFSPDVWDSFSYFDATYDDGSH
                     SEPEGPSGTGKSPMEQKPYDPGEQALTREAEQYLSALKQRTKSDWETIEEKAERIRRI
                     ALFESHAVGEDDYTIYQRFYQPVAGLIGVARKNIQQALQKSRGSRDLNELMTGDDIDE
                     ENLAAVRTTMRIFRDREREPDRTLWCLSLLIDASSSMHDETVAKKLQATIQAALLFGE
                     AVTRIPDIRFEIAAFTDSEYIPLKRYLDDWNVHQGCYLIRQVIHATGGTNDVGAVSSA
                     LERMNRLRMGTGTNRMVFVISDGQSGVGGREQMRAILRTNRDTRIFGWGIGPDMEQVE
                     VTYRPYGTWVRDIADLPQSLGEVLRRELGRPAMAGWREGRSISVEESCQD"
     gene            36543..>36878
                     /locus_tag="PK154_00355"
     CDS             36543..>36878
                     /locus_tag="PK154_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09544.1"
                     /translation="MDAIQVTSLTKKFSDLVAVDNVSFTVRPGEVFGYLGPNGAGKTT
                     TIRILTGITTPTSGTARIFDLDIVSDTIRARRQMGIVYETSNVYDDLTAWQNMMFAGE
                     LYNVGKKDRE"
BASE COUNT         8028 a        10777 c        10174 g         7899 t
ORIGIN      
        1 gtctacgttc tggacgactc gatggccacg atcggtgaac tgacccacat tgccgagcag
       61 gagactattt actcgacccg gtttatcggc gaccggctct acatggtgac cttcaagcgg
      121 atcgacccgt tctttgtaat cgacctcgca aagccagaga acccgaagat cctcgggaaa
      181 ctgaagatcc ccggctactc cgattatctc cacccgtacg atgcgacgca tatcattggg
      241 atcggcaagg agaccaccac caatgactgg ggtggcgtct ccacgagcgg tatcaagctg
      301 gccctctttg atgtctcgga tgtgaccagt cccaaacagc tggacaaggt ccagatcggg
      361 gatgccggct cggactcggc agcgctctct gatcaccacg cgttcctctt cgacaagggg
      421 aagaacctcc ttgtaatccc tctccgggca gtctctaacg ctcccctggt aaaaggtgac
      481 tactccacca gccagccgca gatctggtac ggggcatacg tgttcggact cactcccgag
      541 accgggttca ccctcagggg aaccgtccag catggtagtg gtgacagcgg gtactactat
      601 tacggcagct caacctcgga ggtgaagcgt tccctgtaca tcggggatat cctgtacacg
      661 atgtcttcaa aacagatcaa ggcaaacccc cttgacgatc tcaacacgac gatcaccacc
      721 atccccctgc ccaatggtgg agacatctta taccctcccc ttgtgaagta gaggcaggga
      781 atcaggtcat ttttttggga catgccggat tttctctcac aaagaccgga tttacggcac
      841 cactatccgg gcggcaccga tctgaggaag aaacaggttc acaatcaatg ctccagaggg
      901 atgaaaggaa gatgaggagc aggatgatgt cagcattgtt atcctctctg ttgggtgatc
      961 cttaccagaa ctattccccc ggggctatca gagaacagcg catggaattg cgatatgcag
     1021 gaagatcatc catcgggatt tattatcctt tcaaggaaca ttcagaaact gataccacgg
     1081 acgtcgttaa aggccgtacc tcccttcttt ccctgaaggc ggggaaaacc ccctgctgac
     1141 cggttcccca gaaggatctt ttattctctc atcaggtcta ttctctttga gacatctgcc
     1201 ggcgaaggag tgaaggtatg gatctgaaaa gagagaagac ccggttatcc gtcaggacaa
     1261 aaatatttct ggtcttcctt atcctctctg tcaccgccct tctcctcacc ggttttcttg
     1321 ccttcgtgca gatggggaac gtgagccggt tcgctgtcga tcggagcacc gatctcgcca
     1381 gctccatcat gaacgacagt tctgctgccc tggagcagga tgcagaagat gctcttctcc
     1441 ggctggctac ggaccaggca cacatcagtg atatcctctt ccgccaggtg ggcagtgaga
     1501 ccagctccat ggccacctat gccctccgga tccagcagga tccctccatg atacgggaac
     1561 gacacttcta tctccaggac gaccctccga tcgatcatct ctcgacatcg gtgctcttcc
     1621 tctctcccgg ggtggaatcc catattccgg tggaagagcg caacgccatc ggaatgatgg
     1681 attccatctt catcccggtc tattcctcgg acccgaacct ggcggcggtt tatatgggga
     1741 cgagcagtgg gatgtctgtc atctatccct ggtttaccgg gatggatgct gcctttgatc
     1801 cccggacgcg ggaatggttc aggcaggccg agcagaccgg gaacctcacg tggtcaaagc
     1861 cctacgtcga cctgctcggg catggtctga tgatcacctg ctcgaaacca gtggccgatc
     1921 ccgagatggg atggttctgg gtggtcgggg ccgacatcac tatcgagacc atcaaccaga
     1981 acatcatcgg gacccaggtc ggtgaactgg gatatgccat gctgatcgac tcggagggga
     2041 acgtcatctc tcgacccggg ctctcctccg gggacatgcg gtgggacgag tcgtttgaga
     2101 ccgagaacct cgtcatatct gaaaacccgg gcctccgggc ggtcgccaag gacatgatca
     2161 gcgggaatac cggtgtggcg aaggtacagt acgagaacgg agaacggttc atcgcctatg
     2221 ctccggtaga gagcgtgaac tggtcggttg ccgtggtgat gccggtggat gaagtcgtgg
     2281 ctcccgcgga agcgacgagg gagagtatcc ttgcagcttc gggtgagacg gcatcgcaca
     2341 tcaccggcca gcaggaccag atgcggacga tcttctcggg tgccttcttt gtgctgctcg
     2401 cggcggtcgg aatcctcacc cttctcttca cccgtcacct cacccggccg atggaagagc
     2461 tcaggaaagg atccgaggcg atcggggcag ggaacctcga ttatgccgtg aacgtgcaga
     2521 gtggggatga attcgaggat ctcgccgctt cgttcaacca catggcagct gacctgaagg
     2581 atcatatcga gaacctgaag aagaccacgg cagagaagga acgtatcgag aaggaactcg
     2641 agatcgcaaa agtgatccag cagagtttcc tcccggagac agcgccacga cttcctggca
     2701 tggaacttga aggcttcaat ctcccggctg cggaggtggg cggcgacttc tacgacttca
     2761 tcgctctcga tgatgaacag tggggactgg tcatcgccga tgtctctgga aagggagtcc
     2821 ctgccgccct gttcatggcc ctctcccgca cgctcgtccg ggcaagcacc tcccattacc
     2881 aggacccggc agagtcgatc cgggaggcca acctgaatat cttcctcgac tcaaagacca
     2941 gcatgttcgt cacccttttc tatgcggtcc ttgatgccag cagaaaggta ctcacctacg
     3001 tcaatgccgg ccacaacccg cccatgcttt tctcatccgg gacgtcaggg gtgacgctgc
     3061 ttgaggcaca ggggatcgct cttggggtga ttgacaccat cgatctcgaa tcggtaaccg
     3121 tccccctgca tccaggagac gtggtggttt tgtataccga tggagtaacc gaagccacca
     3181 acgatcggga cgaggaatac ggggtgaccc ggttacgtga tatcgttatg gcatcacgcg
     3241 acctgcctgc gagggagatt atcaccacca tcgtccagga tgtgatccgg ttcaccggag
     3301 cccggcccca gttcgatgac atcaccctga tgatcctcaa ggtcagctag tggcaatcgt
     3361 actgttctcc tttgcaattc cgattgccgg actgagagca tgaagggtcc cggggggttt
     3421 ttccggacgg gatcggatcc gggaaaaacg caagggggct ctcttatgca agagtggtga
     3481 aaagagtaaa aaatatacat gattcctttg aaggccacaa gcataaaaga tgaaaatgcc
     3541 ggaggcattg tcaaataaaa aagtcagatc ggatgatatt cccggaccgg gaccgcgatg
     3601 gcctcgccgg caaggatccg tcccaggtca cggaacccga tgacgtacaa gtgttgttct
     3661 atcaacgtgg tcagttcatg cttctccggg ccatgaatcg gggaaagcca tgccgtccct
     3721 tcccgttccc cgcttggatc aaccacatcc accggtttgt ggaagaggag cgatggaaca
     3781 tcatcaggga caataaaata ccgggatccg gcgatatcca tggatacccc tctgccggat
     3841 ctgcgtgcgg tacccgctct ggtataggtg atgcacggtg tctttcccat tttcttcacc
     3901 acatacctat gggatccctg atcatgtaaa ggtggcgtca gacgctgaat tgaatttatt
     3961 ttgacgattg aacaaaaata atcgcgattt caaataagcg atcaagacgg aatcccgggg
     4021 gatgggtagg aacagagcgt atctggcatc gaacgtcggg gaaggatccc tcccattctt
     4081 acaggcacag acccattttc atgaatatga ggagtggtag attttatctc cctgcaccaa
     4141 tatccattca gctatggaac gaagatctgc cttcctgctc ctggtatgcc tggccggggc
     4201 attcatcctg tgttccggat gtacccagcc gacggactcc cttcccaatg ccacgccctc
     4261 ctccaccctc acgatggtta cctcggtcga taccccacta ctccctcccc ccaccgccac
     4321 aacggttacc ccggttgata ccccgataat cccttcctct gccgataccc cttccctcac
     4381 tcccacgaca ctcattccag gagatacgac ctcctgcacg aaggattatg actgtgtgcc
     4441 agcgcagtgc tgccacccga ctgggtgtgt caatatccgc aaccaacccg actgctccac
     4501 catcgcatgc acccaggtat gccagggacc gattgactgt ggtgccggcc actgtggcta
     4561 tgtcaatgga acatgcagta taatcccggg gccggcgtcc actccagccg ggcaggcgac
     4621 cctgaaaatt gcggttaagg atgctccgaa gatcaaggat gggggaacga ttacccatct
     4681 gtggttgaac atcagcgagg tgaatgttca ccgggcagtg gcaaaccagt cgatcaacga
     4741 gagcaacgaa gagatgacag tgattgaatc ggaggatcct gataccgcag ggtggataac
     4801 ggttgtcaac gataccacca tggttgacct gatggagttt gtgaacgtga gcagggagat
     4861 cggtcagaat accgtagatc ctggcagata tacccagatc cgcctgaaga tcgactccgg
     4921 taccatcacc gttgatggta tcaaccatga tctctcggtc ccgagcgggg tgctcaagct
     4981 gaaccgtgga tttgaccttg atccggagga gacactgacg ctcaccctcg acttcaatgt
     5041 ggataaatcc gtgatcagga ccggatcgga ccagtacaag ctgaaaccgg tcatcgcggt
     5101 tctctcggat gaacagcagg caagaggatc gtgatctccc catgggacag cgggattgcc
     5161 cgttctgcag ggtggacctg gacgaagtga tcatcgataa tgacctggtt tttgccaggt
     5221 tcgatcgctt cccggtgagc cgtggccatc tcctgatcat cccgaaacgg cattttgcgg
     5281 attatttcga agcttcccgg gaagagaaga tggcaatcct tgaggtcatc gaccaggcaa
     5341 agaggtttct ccacatccgc tttcaccccg atggctacaa tattggtgtc aacatcgggg
     5401 aggcagccgg ccaaaccgtc atgcacctgc atgtccatgt cataccgcgg tatacagggg
     5461 atgtccctga cccgcggggt gggatcaggg gagtcattgc ggggcggcag cacagcggga
     5521 acctggtgaa cgatcagggt gaaccatagg atcatgatca gaggacagtg taccttctct
     5581 cagaggagat ggatcggatc tgcagaagcg aacaagagga caggatgcag agaccatgaa
     5641 acagacagat ttttgggaag gagatgagta tgggattttt cagctggctg aaagacctgg
     5701 ttgcagtacc tgtgcatgat gagccggaga agaagcggcg aaagagagag gatgaagaag
     5761 aggaagagat cgaggaactg gttgcgatcg atatcatctg aatcctgcat atgccatttg
     5821 cgaaggcgtc ctttcattgc gggatagttg tccgaaaaga caataccgcg attggatcac
     5881 cgaaatgaga agggagaatc gactctgatc tcatcgttcc ctggagtgac agggagagca
     5941 tgacctatcg ttgtggtgag aggtcatctc cttcaaggct cccctcgagt gtgaaaacaa
     6001 atgcccctga ggggaatgac cgggaagatc aaaggggatt gataccccat ggttatgcct
     6061 gggtgagctt tcctgcagct tttactcctt cggccagttc tgatccaagt gccatgagag
     6121 aatctggagt gatgaacccg tttgcaagca tgattccatc ctgtgcgagc gctatctcct
     6181 tcagattctt ggcccagaga tgctcgatca gcaggaatcc ggcggcgctt ccattgggaa
     6241 tgtccctagc aatctcccgg atctgtttct ggttgagtcc gaactccttt gacatcgcag
     6301 cagcacccgc ttttgccccg gccacggcac cctttccgcg agcagcacca agcccgatca
     6361 gcgctccgac agcggctccc agtcgtttcc ggtcggcatc ggagagctcg gtgatctgca
     6421 ccgattcgat caggccgttc gcatccttca ccacgccaag gagcccgatc agtcggatgt
     6481 acttcttctc actgagcgcg agcagttcct tgagtaaatc tcctttcagc ttgtgcgggt
     6541 cgggaaatcc gatcgcgacc atctggacag gtccaaattc aaccattctc tttcctcctg
     6601 tatagcatca gataacgaat cctggtatat aaaggtagcc gggacccgcc agtcccaaag
     6661 gaatcctggt ggcaaccggc attgctcttc tcagaatgtt ttttatcccg ttcgttcaag
     6721 gccgagaacc atcagaaacg caagcccgga gcggcgccaa gtaccttatc atccccgtgc
     6781 accctctctt gtgtatggca atcgtggtcg ggtccggggc cgggggggca accgtcgcaa
     6841 aggagcttgc ggcagcagga caccgggtcc tgatcataga gaaggggccg gcgatcgacg
     6901 atatcgatgc ccaccgctat tacaagaacg ttgacgccgg agtgaaactg atgaggacct
     6961 gctgtcttgg cggcacgacg atggtgtctg caggcaatgc tctccgttgt cttgaggagg
     7021 agctcctcgg gttcgggatc gatttatcgg ccgaatttgc cgaagcagaa cgggagctgg
     7081 gggtctgtga gctccctgac gaactgatcg gagaagggac atttcgcctg attgaggccg
     7141 cgtcccggct cggttttcca gtgcagaaga tgccaaagtt catcgatccc ggcgagtgtt
     7201 gcagggacgg gcgctgcagc ctcggatgcc ccgttcaggc acgctggaac gcggtccggt
     7261 ttgtggagcg tgcactaatg ctcggtgcag cgctcgtgac cggccaggca gttaccggga
     7321 tactcaccca tggcagcagg gttacggggg tcaggtgcgg atcgagggag tatcatgatg
     7381 atctcgtcgt tatcgcggcc ggagccatgg agaccccgcg tctcctcgcg ggtatcggga
     7441 tccccacctc cccgctcttc tgtgatacct ttgcatctat tggcggagtc tgccccggga
     7501 tcgggttcca tcaggacgtg ccgatggggg catacatgcc gcacagcagc agcctcatcc
     7561 tgacccacta tgcccgccag ttcgtgaccg ccctcaaagc ggctggtcat gatgcagggg
     7621 agggcgacat ccttggtatg atggtgaaga tcagggatga agcaagcggt tcggtaggcg
     7681 agaccatcga gaaaggggtt accgagcccg actcacggca gctggcatgc ggggcctcga
     7741 ttgctggtgc gatcctcacc gaagcagggg ttgatcctgc cacgtttgtc tcgcttccgc
     7801 tccggggatc tcatccggga ggcacggctc ggatagggtt ctcggtggat acccgactca
     7861 tgacccggat tgacggcctg tacgtcgttg atgcttccgt cctgcccgca gcgccaggtg
     7921 caccccccat ccttacgatc atcgcgcttg ccaagtacgc ggcaaaaagg atggagggca
     7981 cagaatgaga accaggtatc ccggtcgaga gcatcttttt tggagaggaa caatatcagg
     8041 aagctgatgt atgatctctc ttccgtcccg gatacgaaac ctcgtctcat gagggtgctc
     8101 ctacccttcc gctttagaaa aaactgttcg ggctgtcgga ggaaccggtt cgaaatccgg
     8161 ctctctctca ttcgtatctt ctgagagaag gtttggtact gagacggtgg gaaaaaatca
     8221 tgattgcctg tcaaatgaca cagcaaaaaa agatgaaaag ggaggaggat tttcaaaggg
     8281 gacattcagc cctttctccg cacaatgccg aatcctgcca gggtcagcat gatcgctccg
     8341 acaagcggaa tcactgaaag gccagttggt gtgggaggag gggttgattc ggtggggata
     8401 gtagttggaa gcatggtcgg gggcgtggtc tggaggggtg tttgggtcag ggtggatgca
     8461 atgagagaga atgacgaggt tgccaccgcc ttcggcaccg ttactccggt taccgtgaca
     8521 aggtaggtat ccggtccaaa ggtggccgta tcaaaagaga aggaccaggt attcttttct
     8581 cccggccctt tccggaccag ggttgtcccg gagaccccaa agaaggtatt gcctgccttt
     8641 tcttgcgtgg gtccgaagga ctgggagagc acttccacca gcagatcatt gccaggtgcc
     8701 aggttggtgg tgccggacag aatgatcgtg tcaccgatgt gataggtctc caggtccgtg
     8761 atcgtcacct ccggggggac gaccgtgatc tgcccccccg caggaaggat catgaggagg
     8821 gaaaggacga tgacgagcaa cgcccatacc ccgctgatct tccggaatcc cggctttgtc
     8881 ctgcttccag gtacggtccg tttcattggt gcgttccact cctgttccag ccggaatcgg
     8941 ctgtgtgttc ctatcaggaa ctgataggag tgaatacgga atggtctgtt aaatacgtga
     9001 cggtggattc ccgtttcacc ggagcgatcc cccacaatac aatctcttct tcacaaagga
     9061 cggaagattt ctctgatgaa aattctcaca ggaaaaatgg actgccccct ccatcacttc
     9121 gaaaattccc gcactcaacg ccatgtttaa tcaaagggac gccaatatcc cgatcatgaa
     9181 ccagctcgcc atctcgctcc tgttgctgtc atccctggtg attgccgccc tctccctggc
     9241 cggctgcacc agccccggga cccttccgga agaattgcca ccggaaggga tcacccctga
     9301 tactactgat cctcccggaa ctccccctgg aagtccgcag agcatcgttg ctgcaaacaa
     9361 tctgtttgcc ctggacctct attcccgcct tgcaggagac cccaaaaaca gcggggagaa
     9421 tctcttcttc tcaccattca gcatctcatc ggcgcttgcc atcacgtatg agggagcccg
     9481 tggcgatacc gccagcgaga tccgggaggt attttatttc ccggcagatt ccgaaggaat
     9541 gcgggcagga taccaggcga tcattggcgg aatcaacaac gggtcttctg cgtaccagct
     9601 ccggaccgca aacgccctct gggcggagca gacctacccc ttcctcccgg gatacatctc
     9661 agtggcagat gaagtatacg gagccagggc gaccaacctg gacttcatca caaagcccga
     9721 tgattcacgg gttacgatca atcaatgggt cgaggagcag accaatgata agatcaggga
     9781 tctgctcccc gccggctcca ttaactcgct cacccgcctg gtgatcacca acgctgtcta
     9841 cttcaagggg acctgggaga aacagttcga cgagaaagag acgagcatcc aggacttccg
     9901 gaccagtgac ggaacgatcc tgccggtggc aatgatgcag cggaccgaca ccaatgcgac
     9961 gtatggttac atggagaccg gggatctcca ggtgctcctg atgccgtacg aatccgactc
    10021 aggcaaacat ctttccatga tgatcctcct ccctaaggat ggagatctgg ctgaccttaa
    10081 gggatcgctt tctgccaaat ggctgaaagg actgcagcag aacctaacga cccagcgggt
    10141 gatggtgtat ttcccaaagt tcaccatgga gacgaagtac accctaccag ccaccctctc
    10201 ggccatgggg atgccaaccg cgtttaccgc ggatgctgac ctttccggga tggatgggac
    10261 gagaaacctg ttcatcggtg acgtgatcca ccaggcattc gttgacgtga acgaagaagg
    10321 gaccgaggcg gcagcggcta ccgcggtagt catccaacgt gcaatggcac ccgcgggacc
    10381 actacccgtc cccgtcttca gggcggatca cccgttcctc ttcctcatcc aggacgacga
    10441 aaccgggctg atcctgttca tgggacgggt ggagcacccg gaatcctcct gatttttccg
    10501 aaccttacaa agggattctc caatctgcgg cgagaatatc aacgaattac acctgccgat
    10561 catctcgcac gatctgccct tttcctgtat aacaaatccc tggaatcgga tcatctgttt
    10621 taaatccaaa tacaggagaa cgctgtagta tagaggggat ccccttcccc tgaaataatc
    10681 catgtacaac gcgatcaagc accggacctt tgaaattctt gaacagacca gggaacgcga
    10741 tctgaccagc cagtatttcc agatcttcat catcatcctg atcgtcctta acgtcatagc
    10801 cgtcgtgctt gaatccgttc aggtccttct cctgcagtat taccggttct tccttgcctt
    10861 tgagatcttc tcgatcacga tcttttccat cgagtatctc ctgcgactct gggtatgcac
    10921 cgaatctcag gagtatcacc ggccggtcat cggccggatc cggtatgcac tcaccccgct
    10981 tgccattgtc gacctgctgg ctatcgtccc cttcttcctc ccgttcctca tcccggtcga
    11041 cctcaggttc ctccgggtgc tgcggatcat gagaatattc cgggcattga aactgatccg
    11101 gtattctgcg gcttcatcat tcatggcacg ggctctccgg agggagaagt acgtcatttt
    11161 cgtgagcctc ctcatcctgg cggtcctttt tgtgcttgcc tcgaccctga tgtactatgc
    11221 agaatacgat gcgcagccgg aagcctttgc cagcatcccc catgccatgt ggtgggcggt
    11281 cgtcaccctg accacggtgg gatatggcga tatctacccc gtcacggtcc agggcaagat
    11341 cattggtgcc gttgttgcca tcctgggtat cggttttttt gccctccctg ccggtatcat
    11401 cgcagcggga ttcatctccg aggtaaggaa ttccgggtgc agggaagata caaccccttc
    11461 tgatgctcgc ctgacctcac tggaacgatt ggccgatctg aaggcgaacg ggtacctcac
    11521 cgagggagag ttccagcagg agaagcagaa gatcctggaa gagaaatgat accccttttt
    11581 cagcggtgaa caattcgcgg taggtgcggt atgatcctat cgtgcaggaa cacgcgatgg
    11641 cttaataaaa cctgttgatt tatgttccat tatgctacct tcccgaacct cccttttcca
    11701 cggtctgatg cttcttttcg taggactgat gctccttata tgcggctgca catcgctccc
    11761 tcctggcgat gcaggaattc ctatcgagag tcttactatc tataccgagc agaacccgcc
    11821 ctacaactac gaggagaacg gaaccctgca ggggttgtca atcgacctct ttgaggcggt
    11881 caccgaacag atgggcagac ctgtatcccg tgatcagata caactggtcc cctggtccga
    11941 gggctaccag gcggccctgc acggcaacaa gaccatgatc tttgctattg ccaggcttcc
    12001 tgcaagggag acctccttta aatgggccgg gcccatctac ccctatacca ccgtcctctt
    12061 tgcccggcct gacagcggga tcgccatcac cagtactgaa gagttggagg gataccggat
    12121 cggagtgata gaagatgacg ccgccgtcca gcagctgctg gatgccgggg tgaaccagag
    12181 ccagctcgtg ctggagacaa atgtctcgac acttgtcacc gaactccagg aaggcgggat
    12241 cgacttgtgg gcttattcgc agacatccgg caggtatttc gccaggcaat tgaccggcaa
    12301 cgcctatagc ttcacgattg tttacacatt ccccgacatc ccaatctatt ttggcttcag
    12361 ccgtgacgtg cctgatgcaa ccgtccagtc cttccagcag gcactggatg ccctgaaggc
    12421 tgataaggac gtcaggggga tcagcacgta cgacgcgatt atcggacggc acgttcctct
    12481 cgtcgggctc tcccatctgc agtacctgac cgaggagtgg gcaccctaca acttcgagga
    12541 gaacgggaca gccggtggta ttggcgtcgg gatcctggag gcggtcttcg cagacatcgg
    12601 ggtgaacctc tcccgggaag atgtgcagat cgtcccgctt gccgaagggt tccgggaggt
    12661 tcagaacggg agctccgtgc tcttctctat tgtccgctct tcggagcggg atcctctcta
    12721 tcagtgggtc gggccattca cccgcggaag ttttgtcatt tatgccccga tgaaccggaa
    12781 tatcaccatc gcctcacccg acgatctgaa ccggttccgt atcggggctg ttgagggaac
    12841 gatcgagaac accctcctta ctgaccgcgg cgtgaacacc tcccggatca tcaacgcccc
    12901 ggcgcctgag gatctcctcc gtatgcttga ggcaggggag attgatctct gggctaccgg
    12961 cgatcttgcc ggccggcacc agatgatgaa gacagctgag gatccgacta cctatgagat
    13021 cgtttacacc ctgagcacga acgacttcta cttcgtgttc agcaaagatg ttccggatac
    13081 cctggtgaat gcctttggcc aggcacttga gaacgtgcgt ctccagcggg atgcccaggg
    13141 ggtcagcgaa tatgagcggg tcatctatca ctaccttggg gccagctgtg cacgccagac
    13201 cttcactgat gcagaagtga tagcccttgt gaatatcacc gctgccgata tcgggcggaa
    13261 cactcccgac acgttccggc gcatcaatgc gggcgaagcc ccctacaaga gccccgaaca
    13321 ccctgacctc tatgtattta tatacgatac gaacgtgacc ctggtggcca gcggcgacaa
    13381 tatccgccag gttggtgtca attaccatgg caaaaccgat gtaaccggaa gaccgttccg
    13441 tgatgagatc gtcaccgcgg cgatagcaga cgggacaggc tgggtggagt atgtgtacag
    13501 caatcctctg cagacgggag tattctacaa gaatacctat ttccggcggg cggtgggaag
    13561 tgatgggaag atctatatta tctcggcagg gaactaccag agctgcgaag aggtatagca
    13621 gcgggtgata agggatgggt cctcccctta tcccctggtc ccctgcaacg acaggtatcc
    13681 ctaaaaaaga tccgtgtcct tttttctagc ggagatcatg cgggtctctt gatcctgaga
    13741 ttcgggtgat ggtaccgctg ccgctgctcc gtcttcttcc ctgcctggat cgccagcgga
    13801 gggcagagct ggatgcaggc catgcactgc tcgcagtgat ggagccagac cggccgtccc
    13861 tcctccagcc ggatattctc caccgggcag accttcgcgc atatcccgca ggaggtgcac
    13921 cggtcatcga cactgaactt ccggtcagca tcatggaccc ccatgatgaa ctttggatag
    13981 atcaggtgct ggacaagaga gccgaacacc ggcatcctcg gtcgtttctg gactccctgc
    14041 tccaccatcc ctgcaatctc ttcgatgcgg cgatcggcct tgccgaggat ctttccgatc
    14101 gtggcatcgt tcggcggatt atacatcagg acatagttcc caggcatctt caccgtaaag
    14161 cccgcatcaa gccctctctt ccccggtccc tgagtcagga tccgttcgag ctggtgcagg
    14221 gccggggttc ccccagaacc tcccatggtc agtacggcaa atgagtaggg gacccgggcg
    14281 aggtcgagcc ggagggcaaa gtctgcaaca acggcgggga ggcccgaaaa gtacacggga
    14341 gcgatgatgc cgacacggtc tccctccgga gcgatccggc ccggatctct ggccacggac
    14401 gcaacaggca cgagttcgca gtcggggagc cgtctgcata atccttccgc tacggcaagt
    14461 gaatttcccg tgcccgtaaa gtaatacagg atggtcttca tcggtgattc ctgagaggat
    14521 ttttggtatc cccagggaaa aggatggtgg tttgcgatcc cttacagggg gctctcagtg
    14581 gagaattctg gggatattta tcctccacgg gcagcgagct tcatcgctct taccagatcc
    14641 ccaatcctgt ttccgggtcc tgaaccggaa gatcccctat caccgatcat cctgatcagg
    14701 gcagaaccca cgataacccc gtcagcccct gcgtgtgtat accgtcggac atgttccggc
    14761 gtagagatcc cgaatcccgg cacgaccgga agagacgtcc tctctttgac cactttcagg
    14821 aggtcgatag actgcggtga gaggtcctcc cgggtcccgg tcaccccgag ggcagcaacg
    14881 aggtaaatga atccccctgc ttttgcgaga atctgttccc tgcgttccgg ggaggtggtc
    14941 tggctgacga agaggatggg atcgatcccc gccatccgtg cggcagcaca gaatggatct
    15001 gcctcttcga gcggcacatc ggcaaccacc accccatcgg ctcccgcatc tgcggcatcg
    15061 cggtagaatt tccggatccc tctccggatc acgatgttgg catacgtcat gatgagcagg
    15121 ggaatcccgg tctctgctcg tacctcccgg accaggtcaa acagccggtc tgtggtcatt
    15181 cccgcggtac gtgcacggga gccggcctgc tggatcacgg ggccatcagc caccggatct
    15241 gaaaatggca tgacgatctc gagcatatcc gcccctgcgt caaccagttc ccgggagatc
    15301 tgcagactct ccgcaaagga aggatcaccg gccacaacac agggtacgat gagcgggcct
    15361 tcccgcaacc ggaatgcacc atcgatacgg gagttcatgg attacccccc atcagccctg
    15421 caacctgggc gacgtccttg tcgccccggc cggaaagatt gataatgagg atatcatccg
    15481 gtccccgacc cccggcaatc tgtcgcgcca gtccaaccgc gtgggcagat tcaagggccg
    15541 ggatgatccc ctcggtacgg gagagataca ggaatgaatc caccgcttct gcatcggtga
    15601 caggccggta gatcacccgc ctggtgtccc gaaggaagct atgctcgggc ccgactccgg
    15661 gataatcgag gccggcagag atgctgtggg attcgaggat ctggccgaac gcatcctgga
    15721 tcaggtaggt gtacgatcca tggagcaccc cgggtgtacc cccgcaaagc gtagcaccat
    15781 gccgcccggc caggccttcc cctccggctt ccgcccccca gagctccacc gggtcatcga
    15841 ggaagggggc gaacatcccg atggcattgg atcctccccc gacacaggcc accatggcat
    15901 ccgggagtcg gccttccttt gccatcacct gctcccggat ctcgttcccg ataacggtct
    15961 ggaactcccg cacaagcgag gggaagggat ggggaccgac gaccgagccg atcaggtagt
    16021 gggtgtcctg cacgttcgtc acccagtccc ggagggcttc attgatggcg tccttcaggg
    16081 tccgtgatcc cgacgcgacc gcactgactt ttgctccgag catatgcatt cgtgcaacgt
    16141 tcagggactg cctccggata tcctcctcgc ccatgtatac ttccaccggg agaccgagca
    16201 ctgccccggc aatggcggta gccactccgt gctgcccggc cccggtctct gcgatgatcc
    16261 gctgttttcc catgcatttt gccaggagcg cctggccaag tgcattgttc agcttgtggg
    16321 cgcccgaatg cagcaggtct tcccgcttca ggtatatcct gcagttgagg tctgccgaga
    16381 gattccggca gaacgtgagc ggggtgggtc ggccggcata ctcggtcagg taatatttca
    16441 tctcctccct gaactcgtcc gatggtatga tcgtccggta cgcctcttca agctcattga
    16501 gggcatacat caggctctcc gggacgaatt gtcctccata ctccccgaat cgtgacgttg
    16561 atctcactcc ctcacctctt ttgcccgctg gataaatgct ctcaccttct cacgggcttt
    16621 tctccccggt gcatattcca ctcccgttgc cacgtcaacc cctgcggggt gcacggtcct
    16681 gatcgctgct ctgacgttat ccggggtcag tccgccggca agaaagacag ggacccgtgc
    16741 cgactgcact accgacagcg cataggcgct gtcatactgc ctgccgctcc cgtggctggc
    16801 atcgatgacg aggacatcgg catcatcggg tatccggtcg cccgggcgga tactccggaa
    16861 taccatggcc gtgcaggagg ccggaatcgg tatggccggg gggacctgga tggcatccgg
    16921 ggcaaggtca cagagtgtct ccatctccct gggagatggc ccatgggata cacaacactg
    16981 gaacagcgtt gggactgcat cgaagatctc ccggatcatg tccggctcga tgcaacgggg
    17041 ggaggatgag aaggcaatca ccccgatcat atcagcccct gcctcctgtg ccatgatcgc
    17101 atcaccgaca ctcgttatgc cgcatatttt tacccgcgta tcaatccctc caatcgttgt
    17161 ccgggattct gagcccgcat gatggtgctc ccgaccagaa atgcatcggc agacggcctg
    17221 aggatcctga tgtcatacgg ccagagtatg ccgctctcgg acaccaccgt aatgcctccc
    17281 tcgcggagca ggggtgacaa tttcatcgtg gtaccgagat tgatcctgaa cgtcctgaga
    17341 tcacggttgt tgatcccggc aaggcgggtg ccggtcgaca ggacttcctg tacctcgtgt
    17401 gcagcatgca cttccacgag cggttcaagt ccatatgcaa acgcgaggtc taccatatca
    17461 cccagccggt ccccgagtac gccggctatg agaaggaccg catcggcagc aagcgcccgg
    17521 ctctctgcaa gctgtcgttc atcgatgata aagtctttcc ggagcagcgg gagatccacg
    17581 tgcggccgaa tcgcaggaag gaacgccgca tcgccctgga aatagtacgg ttcggtgagg
    17641 accgagaggg ctgtggcacc ggcacgggca aagtcccggg caaaccccgc cgggtcaccc
    17701 cctctccgta tggtcccggc ggaaggagag gcatatttta tctcggcgat aatccggttt
    17761 cccggtttct tttgatgaat cgcctcattc aggctgcggg aggaagaggg gggaggcagg
    17821 ggatactcct ctgcccgggg caacccggct acccttcctg cagttgcccg gaggacctgt
    17881 tcgagcatca tgggatgccc ctggaagcac ggatcagtgc cgcgagacgt ccggcagctg
    17941 ctccggaagt gatcgagtct tctgccttct caatcccatg gccaaggtcc tttgccccat
    18001 cgccgaggta gatggcagcc cccgcgttca tgaccaccac gtcatgggcg ggcccgggaa
    18061 ccccggagag gatcttctgt atgatcgctg cattgaactt cggtgaactg ccggcaaggg
    18121 ccgagtgcgg ggaccggggg atgccgaacg cttcaggggt gacggtatac tggtgtatcg
    18181 ctccctggtc aatttcagcc accagggtcg ggccggagac cgcgatctca tcgagcccct
    18241 ggccatgcac caccatcgcc cgctcggtgc cgagtgcaag gagggcatgg gagaaccggt
    18301 cgacaagagc aggactgtac accccgcaga gccgagccgg tgccccggcc gggttgagga
    18361 gcgggccgag caggttgaac accgatgaaa accccagttc cttccggact gccgcggcat
    18421 gtcggagggc cgggtggaag gcaggggcaa agaggaaccc gatgccggtc tcttccacga
    18481 ttgaacagac ccgttcaggt gggacgtcga tccgaacacc aagggcttcc aggacatctg
    18541 ccgatccgca cgagctgctc acgctccggt tcccatgctt gaccactggg atcccggctc
    18601 cggctgcaac aaaggctgct gccgtgctga tgttgaaggt cccggaacca tcccctccgg
    18661 tcccacaggt atccacgaac cttccgcgga cctgcggctt tatggtcagg gcatggcgga
    18721 gcagcacctg gacaaagccc gtgatctcct ccgccgtctc ccctttgatg tgaagggccg
    18781 tgatcagcga ggcgatctgt gccccggtag cctgcccggt ggcaatcatc tccatgaccg
    18841 ccgctgcctg gtcggactcg agcggttctc cgtctgctag acgggcaaga attgcctgca
    18901 tcatgcccct ccacacgatc ctaccaggaa attcttcacg aggagttttc cggttggggt
    18961 catgatactc tccgggtgaa actggacccc ggtaacccca aggtcgggat gcgtcaccgc
    19021 catgatacac tggtcatccg aagagacggc ccggacctga aaccccggcg ggagagatgc
    19081 aggatccacc atcagtgaat ggtaccgcgt ggcggtaaag ggagacggga tcccggaaaa
    19141 aatgccctcg ccggtatgga caatgctgct ggtctttcca tggacaggaa ccagcatccg
    19201 ggatatcctc ccaccgaacg tctggacaat ggtctggtgc ccaaggcaga ccccgagaat
    19261 cgggatgctc ccggcatact ccctgatcac gtcacggcag atcccggact catccggcgt
    19321 tcccgggccg ggcgagagga taatccgttc agggtcctgg tccctcacct cctcaagcgg
    19381 tgcatcacag gggatcggga gaggcacact ccccagctgg ccgaccagct ggtagaggtt
    19441 ataggtaaag ctgtcatagc agtcgatgat agccaccctc atggtccacc cccgctttcc
    19501 ccgacagcac tcaccatagc ccgggctttc tgcacggtct cctcgaattc ccgatcaggg
    19561 atcgagtcag ccacgatccc ggctccggtt gaacactcgg cccgaccgcc cttcacccgg
    19621 acggtcctga tggcgatggc aaactcgagc aggtcgtcaa acccgcaata cccgactgcc
    19681 ccggcataca accccctcgg ggatgcctcc aggtctgcga tgatctgcat cgcacggatc
    19741 ttcggtgccc cgctcaccgt tccggccggg aaacaggagg caagggcatc gaaccggtct
    19801 tttcccctgg caacgatccc gcagacccgg gagacgatat gctggacatg cgagaatctc
    19861 tcgatctcca tgaattccgg gaccgtgacc gagccgaagg ttgagacacg cccgatatcg
    19921 ttccgggcga gatcgaccag catcaggtgc tctgcccgct ccttctcatc tgcgagaagt
    19981 tccttagcaa ggcggctgtc ctcctccgga tcctttccgc gtggcctggt gcctgcgata
    20041 ggcacggtct gtaccactcc ccgctggact ttgaccagca tctcggggct tgatccgacg
    20101 atcgcctcgt ccccgaagtt caggtaatag aggtagggcg aaggattgat ggaacgaatc
    20161 cgggaataga tatcaagggg gttaccattg aacggcaccg taatcttccg tgagaggacg
    20221 acctggaaga tatctcctgc catgatgtgt ccttttgctt tccttaccat ctccaggtat
    20281 cccacctggt cctcctgccc cggatccggc aatgccccca tcgcagggct gcaagtgccg
    20341 gtgccagctg gtattgggat atcctgtatc cttttttcca gggcatccag tcgctgctgt
    20401 gccagacgta tcgcctcgtc tccctcatat ccgtgaggaa ggaggatatc ctcaaagagg
    20461 aggcaggacc cctgcacatg attgtacacg atgccccttg tcgaacacat gaaccgggca
    20521 aggggaatat catcccttcc tgcctggaca tgcccacccg aaagactggt caccatgtca
    20581 taactgcagt acccgacaaa gcccccgtca aaatctccct cgcctcccgg tgtatacgag
    20641 aaccgcgagg acaaagaccg gagctggctg accggatcat cccccaaaag aggagggaag
    20701 agggaaccgc cagggtatgt tccggaaatc gccggagatt ctccaaggac gatctcaaac
    20761 tccggttcca taccgattat ggaacggacc gcccttcttg ggaccccttc catcgattcg
    20821 aggatatatc ccttggtgat cttcagggca caaaagaggg taaacggatc gactcccgga
    20881 agaggtatga tcctctttag ggggataacc tggaaaggag ggtattgttc gggctgatgg
    20941 ccattcactg ccgtcctggg gacggcattc cccagtccag cggcatcacg tacttggtat
    21001 tcatgtacaa ataagtacat tgatggattt atttatactt ttgtatacct ttttggatta
    21061 tgaaatttca tccccttacc accacatctg aagagaaacc cctgtggatg atccaccaat
    21121 ggcacggggt tgccggccac tccctggccc atcaccctaa agcgatcagt ttaacgttct
    21181 tccccatggt attgcaatgg gagaactgct gaggagagtc atggccaaaa aaaccaaggg
    21241 tacgaatacc aggaatggaa aaaatcgcgc gttattgatc ggtattttca ttgtgatctt
    21301 ccttataatg gccgggatct tctcctcctt ttcaaaggtg aatgacaata ccgatacggc
    21361 ctcttggtca ctcgatgaat ccagcctcct ctccgtgagc ggccgtaccc tgccagccat
    21421 cacggtatcc cctctcgaag aaacggtgaa ctgcacgctg gagaagatca gctaccagag
    21481 cgatgacacg atggtgtatg gcctgttgcg cctcccgaga gaggtccaga tcccaccggt
    21541 cgttatcgtc ctgcccgcgg ccacggtcac aaaggaagga gattcggcaa tggcagatgc
    21601 cctctgttcc ttcgggtatg cctcgctcac cctggatgaa cggggcaacg gcggcgaaac
    21661 gcagggagtc tcaccgatgg atcttcaaac aggctacact gcctaccgca acagggagat
    21721 gcccgcccag tatgcacaga tctacgatgt gctcctcgcc tatgactacc ttcaaacccg
    21781 gaaagatctg gatggggatg ctatcgcagt actaggtgag agcatgggcg gccgttttgc
    21841 gatcatcacc gcgggaatcg agccgggtat taagggtgcg tttgttgtca gctctggtcc
    21901 ttacggggtc gatgccggga acaatccgga cgtgcagcgg tttattacct cgatcgagcc
    21961 tgaaagctat ctgctgaagc tgcccccacg gccactggtc atgttccact ttaccgacga
    22021 cccgatcatc cctctcgctg aagggaaggc actctacaac aaggcccttt cacccaaagc
    22081 ctggcaccag tataacggga ccgtgcatgg cgtctacagc gagacctatg ccgaggattt
    22141 gcacgcagaa ttgaaggaga ttttcggaag gtgatccccc taccttctca tttgaaggat
    22201 ggtcacgtta tgagcatcag gattaccaat gttaaagccg ggagggtatc gcatgttcat
    22261 gattagcgcc tgtgagcgat gatacccatt cccgattcac ctgttaatgc tcgttataga
    22321 ggatccacac tacatacaac atcgagagag tcgcaacgag aaagatgatg gctacaacgc
    22381 tcggtaagag ggagatatcc atgaatgcga taatatcgta aacgacataa atgccaaagg
    22441 tgagggcaat aaaccatcca tagatcttct tcttcagaat cccgatagca atggcgatga
    22501 tgcagatgat cgcctcgata acgatggaaa ccggctggag gtagaatgac aggtccatac
    22561 agaagtgagt gctgctgaat gtaaaatagt tccaggggag cggaagataa gacatgccat
    22621 gggtgcatcc cggcaatcgt cctcgtaccc ctcccctaca atccttcgag gagtctcttg
    22681 ctcaccagaa ccccctcaag gtcctttact tcgagaacag ccgtcgccgc cgtccgttcc
    22741 agtgctgcca tgaccggacg gaaatcgatt gtaccttctc caatagcaga atggctgtct
    22801 ttccagccat cgttatcatg gatatgcatg tgtgagaagt cagtctcaag gaatgccggc
    22861 aggcagtggt tcaggttcgc atgcccgcaa tcgaggcaaa acccggtatc gccaagcgat
    22921 gggatgtcat caggggtcct gaaatggaaa taatttaatt cccccatgtt ctcgaaccag
    22981 aacatgagcg aatgatcggc agcagactcc tggagctgat gcagggattt ctcaaattgc
    23041 cgatccgcgg cctctttttc agtgttccat gcatggtacc cgggatgcat cacgaccggt
    23101 gcccctatct ccgctgcgat ggcaaaacag tcgatcatca cctccacgct cgccctccgg
    23161 atactctcaa agaggctcgc aatattcatc ccgtggtagg gggcgtggat gacaaaatcc
    23221 tgggaatagc tctcaaagac tgccgggtcg gtgatgagat gaggcccctc gtccatcacc
    23281 tcgatcaggt cggtgatcca tgagagcgta tcgagggtct ctgccaatgg gcgaccgatc
    23341 aggcaatagc ttgagatgcc aagctccatg aggatacctg agtggcgagg tatgaaaaat
    23401 gtgcggataa tggaatgagg gtcagcgctg tatggatcca gggattccaa aagggggtcc
    23461 tccggtcagg tgaaacgatc tggaaggcag ggagagcagg gggacttccc ctcgtgccta
    23521 agagatgaat gaaaatcgat tgagtgtaat catctaattc gtcaatccat tatgataacc
    23581 tcccgattga gaaatctttt aaagtttgag gaaagaagtg gatgcacaaa aagggatgcc
    23641 acatgaccgt acgagtcctg accacgatcc tcctgctcct cttcctggca gctgctgccg
    23701 ggatcgttgc agctgaggaa gagaccacct actattgcta cgattactcg acccagtcct
    23761 ggagggtatg cacccctgag gaggctatcc aggcaatgga tgccctggaa aaggagatcc
    23821 cgaaagagct acctcatgaa gaatgggaag ccgttatcaa ttccaaccag ccatactggc
    23881 agtactatct cgatccgccg accgggacgc ccaccccaac gcctaccccc acccccacac
    23941 caaccccgac tcccacacca cggccaacgc tctccccttc gctcgaacaa tcgttcttca
    24001 ctccgtggtt cagcaatgct cccggtgtaa tcccgacctc tgcaccaatg aaatattcag
    24061 ggagaataac aatcccgaca aaatacccaa taaccagtac ccccacccca gtcccgactt
    24121 ccactatatc ccctgagacc acaatctcga aggatccggg gaattcgttc ttcagcccca
    24181 tgttcccggt gaggaatttc tcgctgttca agtggttctg aagatccctc gtcccctcgc
    24241 ggccggctga acagtgaaca tacccggcga ccgggatgta tcctgggcac ccggtcgcaa
    24301 cggacctgga tcttcttttc atgaaagcgc tcaccatgaa caggaaggga ggaattttac
    24361 cgagtctctc tcaaataaca gcaatcgatg aagaatagcc gagatctacc ccttcctgtt
    24421 gatctcgatc atccggtcca tatatgcatt ggcattgtta ataaacatgt caacgtattc
    24481 tggagccggg atcaatggca gttcgagccg gaacgttgtc tccggggtaa tttttccttc
    24541 tttcaccaac tcccatcctg ctttccggag ctgcatcttg taggcatcga tccacggttt
    24601 cagttcttca tcctgaagca gggacccccc tccccggtag atctcacccg cgagcatggt
    24661 gtcgaaattc ctggccaacc tttgagcgag gaggcggaga acctggaaat gcgtctgttc
    24721 gggaaagccg gaatttgcca tcatgacaaa tttctggttc ttcttctctc ccgaaacatg
    24781 ccgaacctcc ccgtggggat ctttctcgaa atggggatct cccatcggaa tgagccggtc
    24841 catgaagttc ttcatgatcc cggaaacatt gtcgatatac agcggcgttg caaagatgac
    24901 gatatctgcg gagcggtatt tcttgatcaa ctcctccata tcgtcagact ggacacatgt
    24961 ccctggtgtt ttcagccagc agccaaagca gccaaggcaa tgcctgatag aatagttcga
    25021 taggaaaatg ttctccgtat ccgcacccgc atcctctgcc ccagcaagaa aggacgccac
    25081 catgacatga gtattactct gttctgcccg gggacttccg ttaaaagcaa caattcgcat
    25141 ctgctacccc caccattatg atgccgtatg aaaggggaat tcttggcaca tcgaataaaa
    25201 gtatgcctat cacataggga gtacagatac gatgggggat ctcagcctgt tggattcacg
    25261 gtggctaccg gtagaaaacc tgttttattt ccgtaaaaac ggttttttac attctgaaga
    25321 ggctgatttg ataaaatcag cgtaagattg aaatattttt acacatctat attcctgcat
    25381 gaaaaaacgt cagatcctca tttccggcct gatagccatt tttttgttta ttgtgtcggc
    25441 gactgcggcg gagtgttccc tggtcggcac atgggggagt attgatggat catacattta
    25501 cgatctggat gagaacggtg gaggccagat tattgcagag ggcgtgatcc aatcctgcaa
    25561 ctggaccgaa gagagcaagg tcgtgacgat cacctggtca tatggtcctg tgccaggaga
    25621 gcggtatatc gacacggtga ccctcctcga tgaatgcgat tcatttgccg gaacgaacaa
    25681 ttacggagca ccgatctttg ctgccaggat agtcagccct ccttttgcat gttcagatga
    25741 gttttctctt cagcagaatt cagcactcgt ggttgaacgg ccaggggtac tgatcaatga
    25801 ttatcagcca aaccaggaga acctcaccgc agttctcctc tcctctcccc tgaacggcga
    25861 tctggtcttc aacgatgatg gatcatttac ctacacccca tcccctgaat ggtgggggac
    25921 ggataccttc acctacctgg tgaagaacga aacagaggta ggaacctctg cccaggtgcg
    25981 gctggttgtc acaaagccgg agaacccggc aacctatacg atcaccacct cgcgtcttgg
    26041 cgctggtgag atctccccct ctggtatcat tactctccag cccggagaga accagatgtt
    26101 cctggtcctt ggggagaaag gatcccggct cacctccttt accattgaca gcgttacgat
    26161 cgatgtcgtc caggaccctc ccagcccctc taccgagaac cagggtgtcg tggatatcca
    26221 gagcctggtc gttacctttg aaaatatcat gtcggaacat accctgcacg cggtattcac
    26281 cacacccacc atctgagaga ggagaggaat cccctctctc tcattctgaa agaggatttt
    26341 ttaacgctga acaaccaaaa gaaggacttt gaaatgaggt gattcgaaat cctggctttt
    26401 ccctctctat cttgagataa atatatatat tttgagttat aatctcatcc ttgaccatca
    26461 atcaaggtgg tggttaatgg agtggggacg cacaccgttt ttaagaaccc tcatgtggca
    26521 cccagacttt tcgcgtgcgt ttccatttca tttcatacca tacttttctg aagagatatg
    26581 atctctgttc gtatctgata tcagaacatt ggacctgacc agaatggacc tgaccagaac
    26641 ctttttccac atatcccctg aactcagatc attatcaccg ttctctcatc agtctggaga
    26701 atgaatcagt gatgacagga catgccgtat ccttccgtgt agcgacgaaa acataatctg
    26761 atctcgcact atcgggaggg acgccaggaa cactatgcga tcgatggaat tgggtgatga
    26821 ggaagaccag cggctggagg agatctggat cactatcctc aggcgactca cccggaccat
    26881 ctcccggacc ccgtccggga taccggaata tcaccctggt cctcccaaga cgccccggga
    26941 atccttccgt ccgctccctc acctcgccgg tgtctatgct tcgtcagacc tggctgccac
    27001 tatcgcaggc gatctgatcg ggcgtgacct ccccgtccag gtcgagcgac atgagacccc
    27061 tcctccgtat tcccaggtta tcggggatca caccggcacc ctggtgcacg gacataccgg
    27121 cccggtctgg gtggtgtgga ccaattacca caacaacagg tgtgagacta tgcaaggacc
    27181 ggccgggaaa gacatgatcc cgccaggtat gaggggccat cagacaagga agataccatg
    27241 aaaggcatga cccatggatt tgccgatgca ttgcgactga cactggaaca cattaccctg
    27301 cttccgaccg agaccgtctc gcttcttgac agtgtcggcc gcattgccgc agaagacctc
    27361 tgtgccctta ttgattcccc ttcgatggat tcctcccgga aagatggcta tgcggtccgg
    27421 tccggtgagg tggctggagc gaccccggat catcctgtcc ggcttcgcct ggttggatcc
    27481 atcgcggcag gaggcgcgaa caatatcggt gtcataccag gtacgacagt gcgggtcctg
    27541 accggagcct gcatcccgga cggggcagat gcagtggtct ccgaagaatt cgccacaaag
    27601 gaatcccacg aggtgctgat agaaaacccg gctgcaccaa aaaacatcct gccccgtggg
    27661 agtgatgtct cgctcgggca gtgccttctc tcaaagggtt catcaataac accggtcatg
    27721 gccgggctcc ttgcggtagc cgggcatagc cggatcccgg tataccgaag ccctgcaatc
    27781 gggattgtcg gaaccgggga tgagatcatc gagcccggag aaccccttcc tgagggcaag
    27841 atctatgcaa gcaacatcat caccctggca ggattgtgcc ggagatacgg gggctcctcc
    27901 accctcgccc tggtcccgga tgatcctgat accatctttc gaaccctgca ccgcctgtcc
    27961 ctggagaccg atgcgctgat caccagcgga ggggcatgga cgggagacca tgacatggtg
    28021 gcctcagtcc ttgggagcct cggatgggag caggtcttcc accgtatccg gatcggcccc
    28081 gggaaagggg tcggctttgg catgcttggg ggaaaacccg tgtttgtcct tcccggtggg
    28141 ccaccctcca atctcatggc gttcctgcag attgcactcc cgggtctcca ggcgctttca
    28201 ggagatgccc atccaggatt gccccggatc cgcgcaaggc tggggtccga tctggtgggc
    28261 ggggatcgtg actggaccga ctttttcttc ggtacactct cactggaagg tgaaatggtg
    28321 gtgttttacc cgatgatgaa acggagccgg ctgtctgcgg tggcgggagc taccgccatt
    28381 ggagcaatcc ctgaaggtga ggactctctc catgaagggg aggtcatcgg ggtccagctc
    28441 atcgggcacg gaatggagcc atgagagggg atccattctg ataccgacag gaaagtcgcg
    28501 ctcgacaatc ggcattcgca tttctttgga gcgaaaaggc cgataaatga gatcccgttg
    28561 caggagggat tctttggaag ggactactcc gttctcgagc agggctgtat ccccctgatc
    28621 ccccgtgaat ggatacgaag atccgtctgg agagacgatc atcgacgcag attggcagac
    28681 gcattcctgg ttggaatggg aggaatcagc aggtatcaga tcttctcccc ggccggtgac
    28741 cacgggatga acctgatttc catcccggaa gaaccaaccc cggactccat gaggatcccg
    28801 ttcattgcag agagaaagtc gttttcgagg ctgaccacga gtatgtcctg ttccttgtaa
    28861 taccggatat cagagatcgg gagaccctgc catcgaactt ccctgccatc cccggcatgc
    28921 aggacggtcg tcccggcagg atagcccgcc tggttgatca caagaccggt gccatcgcct
    28981 tttcggccca cagcaaatga gaagaggcgt tccatcggac ggtactccag ggagatgttg
    29041 cttatctcaa ggtgaacaag gggatcgtga gcctgtccgg tccgtgcata ctgccaggga
    29101 tgccctgact cctggaatga cgcaacgagg tggctaagga tccgatcata ccggtcatca
    29161 tcgatgacct tcagccggcc gaaccggcct gggagcgagg cgagctccag ggttgccaca
    29221 gggttaggtt ggccaagcca gaatccacgg acaatcgggg gtgtaccttt cagccctcca
    29281 ctgctgcccc cttcctggag gaaccggacg atgaagaccg accgcaggtg atccctccca
    29341 ttggagtcgc agaccaggat atctcccctg gagagctggg ttcccctccc gatcggctcg
    29401 gcacactccc ggaactctgc aagtatcgtg ctcatttttg attcagctat catatccctc
    29461 ggctttgcgg tactcaggaa cacggaagcc gcataccgcc gcaaggcctg gaggtgctcc
    29521 tgggtctcgg ggagaagtac ctgcctccgc ctctcttcgc agacatcgag gagagagaac
    29581 gggatcacca gttgttcagc gagcggatcc tgatcccctt cggacgggga cagatcccag
    29641 atgaccggac caaacccgtc cacctccacg acaaacccga tctggatccc tttgccgggc
    29701 atgagggtgt agaggatatc cccgaaccct aaccggacag tcgaacgagg ccgggcatag
    29761 acaaagagct cctcaaggtg gggatatgct gtccgaataa ggggggcaag gatggcagac
    29821 tggtaggatc caggagccat cgatgtgaat cctgctccgc gtatccccct agcagctgca
    29881 cacgtatctt caagaatgag gaagagggtc ccttcatgat aggccaggcc ccggaaggga
    29941 tcgcctgcac agggtatctg cgctcctcct tccctcccac tccaggcaca tatcccgccc
    30001 ttccagtgga ccacaccttc tccgggaata tgaatcgccc atccatccgg agtggcaacc
    30061 agccggaagc ctgccggatg ttcgcggctg acctccccct cacgatacga agggctggtc
    30121 caggggggtg aagggaggcg gcaaagggtc ctgacaaatt cctggcagct cccgcatgcc
    30181 tgccccatct ctctgacgat catcgcaagt gatggcgatg cagggatttt tgtctctgat
    30241 tgggccatct cgtagatggc aagggtatgc atctgatcgg aggcgacaag cacatactca
    30301 cctgcattcc tgtgcaggga gggtgccggt gatgagggga ggtttgtgat cagggctggg
    30361 tagcctgtcc ggtggttcac tgcttcagca gagaacgcgt gtctgccctg cgtgaccctg
    30421 acctccatat caaggaccct gtattcatac ccatggtcca tcaggagcag tccgccaggg
    30481 gagaacccgg ccttgatctc atcgcaggca ttggcgtccc tgccgcatgc aaggaggatc
    30541 tcggcaatcc ggaggtggca tacccagtct cttccggcag ggatgtgggg aatcatctcc
    30601 cttaaaaccc tgattgctgc gagcggtttt cctattgcct ggtaggtctc tgcaagggcg
    30661 atacccttct cctcgtcgcc tttctcctgg tcccacgagg tgcgaaatcg ttcgagtgca
    30721 gccgaggccg caagaacgtt ccggtcgtaa cccgcagccg agagagccgc ctgtaccgca
    30781 tgcagctccc ggccatccct cggcccgtag cagctgcaga ggagatccac caggttgaca
    30841 gccctggacg catactgaag cccccgttcg atcgccgcct cctcgttcct catcgtcacc
    30901 ggttcgaaga gcattccgct gctgtacagg ggatggaggg tataatatcc ctggaccaca
    30961 tcggatacaa agtcgggatc ggctcccgga tacttcctct tcagcacttc gaccgcctgc
    31021 ccgggaggga ggtgaacgat agtatggatg gagaaccggt ccatgaactc gaacgagaag
    31081 tcattcacct cgtaaggagg ccggttgggg ttgatattca cacagattga ggccctgacc
    31141 gggacctctc ttcctcctgg cagcctgacg gatcggtcct ggagcaggtc attaaacgag
    31201 acgagaaccc cgggctgtgc cttgttcgcc tcgtcaagca cgatgatcca cccgtgatcc
    31261 gggtcagtga gggcacgcag accgagcgtc gcggtatccc ctgttttccc tgcctcgtcc
    31321 tcgccaaaac tccggtacgt gaggaggtct gagacccggg tctcgccgct catcccaatc
    31381 acgaggagat tgtaattgag gaggtgggcg aggtaactct cgatcttgtt cttcccgacc
    31441 ccggcctccc cgataagcag gatgttctca gcaagtgcaa tgtctttcaa gatccattcc
    31501 aggtcggcaa ggttctgctg gacctcctca atctcggtat cgggcacgta tggccccccc
    31561 tgaccgaggg gatatgccac cgatccgatc cggagcagcg gggcattacc cccctcttcc
    31621 cggaccaccc gtgaatgctc cctgcgccgg tccccggcat caacaatatc atggaggtcg
    31681 aggagatcga ggaccagttt ccgtgcgata gggggatgaa tccggtcatc gagccagtac
    31741 gccttccgcc agaacaggga acgaagcagg aaaccgtctt cagggaaccg ggcaaggtgc
    31801 cggacgaccc ggatcaatcc acgcgttgtc acggggaaag ggaccacccc gtgctcctcg
    31861 gtataatccc tcctgatatc gttggcaacc gagatcagcc gaacgaggat cgaatcatca
    31921 acccccggtc catagtcctt caacacttcg aactcttcat ccggcgggag gaaccggagg
    31981 tgcaccttgt ttgtgaaccg gttcacaaac tccccgctca tgaccttccc ctcgtaaatc
    32041 cccctgcctt cgctcttctg ggggttgacc gtcgcgataa aacggctgtc ggggtgtccg
    32101 cggatcaccc gctgatggag actttttgcc ttgttctcga gcggttcgtt gaactcggcc
    32161 tggacgtcct ccccggtccg gttgatctcc gagaggacgt tccagtcccc ggcgaccatg
    32221 ccgtccatga catcgctcat cgtccagccc gtcttttctt cgcctgtctc gccgtaatgt
    32281 ctccgttcgg taatatctga cctccggagg ttttcatgga aggttaaaat cctggtctgg
    32341 aacgaggcag ccctctctgc cgcatgggcg gcggcatggg ggtcatggag ccctgccgcc
    32401 atcaccagga gggcctgccg gtatacctcg cccagatacc ggaccagcgt ggtctttcct
    32461 gaccctgact cccctgtcac gagcaggttg ttccgcgacg gcatccggag ggcatcccac
    32521 atctgttgca gcgaggagat agttgagggg acgaggacga tgaagggttc tgccggggca
    32581 gggatacgga tctccccgat ggcaaattcg gtcgcaaccg gcacccggct gccggatcgc
    32641 catgcggtca ccttcagggg tccgaagctg ctcagtcctg gcatgattcc tccacagaaa
    32701 tcgacctgcc ttccctccag ccggccatgg ccgggcggcc aagttccctc cgcagtacct
    32761 cgccaagcga ttgcgggagg tcggcaatat ccctgaccca agtcccgtag gggcgatagg
    32821 ttacctcaac ctgctccatg tcagggccga tcccccaccc gaatatccgg gtatccctgt
    32881 ttgtcctgag tatcgctctc atctgctccc tccccccaac ccctgattgc ccgtcagata
    32941 tgacgaagac catccggttt gtcccggttc ccatccggag acggttcatc cgctcaagcg
    33001 cactgctgac cgcccccacg tcattggtcc cccccgtggc atggatcacc tgccggatca
    33061 gatagcaccc ctggtggacg ttccagtcgt ccaggtaccg tttgagcggg atatactcgc
    33121 tgtcggtaaa cgccgcgatc tcgaaccgga tatccgggat cctggtgacc gcctcgccaa
    33181 agaggagggc cgcctggatc gttgcctgga gtttctttgc cacggtctca tcgtgcatac
    33241 tgctgctcgc atcgatcagg agggagaggc accatagggt ccggtcaggc tctcgttccc
    33301 ggtccctgaa gatccgcatc gttgtccgta ctgcggcaag gttctcctcg tcaatatcat
    33361 ccccggtcat gagctcgttc aggtcccggc tcccccggct cttctggagg gcctgctgga
    33421 tgtttttccg tgccacacca atcagaccgg cgaccggctg gtagaagcgc tgatagatgg
    33481 tataatcgtc ctctcccacc gcgtgcgact cgaacagggc gatccgccgg atccgttctg
    33541 ccttctcctc gatggtctcc cagtcgcttt tcgtcctctg tttcagggcg ctcaggtact
    33601 gctctgcctc gcgggtgagt gcctgctcac ctggatcata cggtttttgc tccatcgggg
    33661 atttcccggt ccctgagggt ccttcaggct cactatggct cccgtcatca tacgtggcat
    33721 cgaaatacga aaaactgtcc catacatcgg gagaaaatga tgtgtcggga acaaaggaag
    33781 ggatcatccg tccccggctg gttgtggggg gttgtatccc gggaccaccc ggtgcttcag
    33841 tcaaccggat cttcaggccc ccctcatcaa aggcggaagc cgatgctgca caacgccgtt
    33901 ggaattcgac tcccgccttc tggagatccc ggaacgcctc ttttgtcatc cccgtgcgtc
    33961 tctctctcaa ggctgaattg gaggcagatg cctctttcag gaattccatc gcttcgacgg
    34021 cgtcccggta ctcccttgcc gctttcctga tgtcccgtga ggtcttcagc aacccgtcga
    34081 gctcttcgcc catcataccc gcaggcgatc cttcgatgct gttggcatcc tcctgcatgt
    34141 cgaggagctg ctgctccagg gtctcagcga gagagtcgat ctgcctggca agccctgaga
    34201 gatcagatat cgtctctccc tggtctggga ttatgttgtc agccccagcc tcgacaccct
    34261 gctcacctgc tccgacaagg ttatcaagga gatcctcact ccggccgcat gcctccacca
    34321 gggcatctgc cgtggcaggg ataccagatc cgtgttcacc ggatccccgg gcgccaggta
    34381 ccttaccaag cgacggacga cccctcccgc tcatcctccc cggcctggtc tcactgaaag
    34441 gagatccccc cggggttaaa atcccatcgc cgtcaccgat catgctcgtt ccgtttcctg
    34501 caagagccct gccggttgct gtatgggaag gctggtgagg gctgggtaca atggtatcgg
    34561 gacggtctac actcccatgg ggagcatagg aaggggattt ttggctggaa tgcggtacac
    34621 acccgccagt cgttcctttt gatgggatct ggcagagatg tgcgaggaat ggccatacct
    34681 gttcggcgat gatctgcaga tatgtttcat cgggagcagc cacggcatcc gtccgtgcaa
    34741 tggctgtggc agcaagggca tgccggactg catcgccttc aatatggctg gccggctgac
    34801 cggtccgtcc ttcaaagagg actccttcca ggaactggtc aggaaggggt aatcgcctga
    34861 tccggccccg gtctgcacag cggttcacca gggaatattc ttcctggcag aaccgatcaa
    34921 tccatctgat aacaccagga tgaagtgcag accccttcca gaccgctctc tggagtccga
    34981 tcaccctgac aaggcggagg aaccagggat catccggcag gtcatgcccg tgaggctgga
    35041 taccgaacca ctccctgtac catgcttcca tcaggagtgc ccgtgtggca tccccgctcc
    35101 ggaaggcaag ctcgtcagga ttatagagga tgagatggcg ttccggccag aggtcaagtg
    35161 tcgggctgaa ggccgggact gtggaaagga tgggaaccgc cctccactgg taatctccgc
    35221 tcaccgcacg gagcaactgt tcctgctcct gcatggtccg atcgaggttt ttcaggaggc
    35281 cgtcacctgg ttcagcacga ccggacaccc ttcctgccag cgtccgtgca cactcaataa
    35341 gaaggctgat cccggattcc tgatcatccc ccgatgcacc ctgtatcatc ccggcaaaca
    35401 ggtggccggc ataaccctcc actcgcccga acgagagatc cttttccaat tcttccctgg
    35461 cgaggattac cgcaaagagc tcccgaatgt cgggaaccat cccttcacgg atgaataatt
    35521 cctcccctgc ttcgaacagt tcgaaagcat cccccccgaa tatacgggta aacgcggtga
    35581 ttcccccatt ctggatcacc ccggtatctg ccgctgcctg gcggataaat tctgaaaaga
    35641 cgggttcagg gatcatccct gcgtgaacga gggagatgaa gagggcagta tacaaatgac
    35701 cggagaagcg agcaggatcg tctgcgtgag gtatcaggat ccggccgtcg tcggcaataa
    35761 gagggggact tcccggtgac ggtatctctc cggcagtttc gaggagcagg gcagccggga
    35821 tgcgctgcat gcgggtcatt cctggactga ggagatcctc cagggacagg ggggtgatgg
    35881 ttgatcggag cgggaggacc acaggttcgg gatcccgggg tgagacggtc ctcccccaga
    35941 ccctgcatga aaagtcgacc tgggcttcct ggatttccgt gaactgtgcg agtatcaacg
    36001 gcgcgagatc ccgcggatcg ccaatagtcc cgtcagtgag gggtgaatgc cgctcccttt
    36061 ccctgatggc gctggtcaac tggtcccggg tgataccggt tgaccggatt gtggcggtgc
    36121 aatcccgtat tccttcaaga gctgtgcgat attcatcccg atttgccggg gtgacaaaag
    36181 catcggtgat cggatcatgc tctgcacagc tgccaagaag catccggagg agccggaaca
    36241 ggtgatggac atgatcttcc atggggatct ggattggacc ggattggccg gggaacgggg
    36301 acgacccggt accagttcat atctattcgc catcttctga ctaatagctt cagatacagc
    36361 tctggttcga accaaaaaaa aagagagagt cctgccatca ggtacattcc cggaaggtcc
    36421 ttttttcctt aatcaggata attccttctg actgacacct ccgtgtattc ccgagctctt
    36481 tttttcatac agcattatca gatcccccac cccaggtatg attaccatcc cgggatactg
    36541 ccatggatgc tatccaggta accagcctca ccaaaaagtt ttccgacctt gtcgcggtcg
    36601 acaacgtgag tttcaccgtc cggcccggtg aagtattcgg gtacctcggc ccgaacggcg
    36661 ccggaaagac gacaacgatc cggatcctca ccgggatcac gactcctaca tccggcacgg
    36721 cacggatctt cgatctggat atcgtttccg ataccatcag ggcacgccgg cagatgggga
    36781 tcgtgtacga gacctcgaac gtctatgatg acctgaccgc atggcagaac atgatgtttg
    36841 ccggcgaact ctacaacgtc ggaaagaagg accgggaa
//
LOCUS       DAOOCO010000003        34532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_928_length_34532_cov_5.667053, whole genome shotgun sequence.
ACCESSION   DAOOCO010000003 DAOOCO010000000
VERSION     DAOOCO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 34532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34532
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_34532_cov_5.667053"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PK154_00360"
     CDS             complement(<1..975)
                     /locus_tag="PK154_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW09545.1"
                     /translation="MKPVRSLIVAALIILVIVGSAAADDCGCGGSWDSPSDDWDDGGS
                     GSFDDDDDSGSSGDSSSSGDSGSSGDSSSSGDSGSSGDSSSSGDSGSSGDDSSSGDSG
                     SGSGGSDPSDGQESTSGSSGSDSSSSDSGSSSGASGESAEEGVLWRIKGDELFTKGQY
                     NESLAAYEKALSSDPFSFRAWKGKGNALLSLNRTVEAENAFGKALKLDPGDASVYALL
                     GDARFAAGAYDDAVEQYLKALAMNPNLEDVAEKLSMAYAGDQSRETMETEASVTESGT
                     PVPTELPTVALINDTATLTAEVTSTELSPTPTAGIAGGVTGIVALMFLL"
     gene            complement(1038..1958)
                     /locus_tag="PK154_00365"
     CDS             complement(1038..1958)
                     /locus_tag="PK154_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPW09546.1"
                     /translation="MFEHSHPVYTFDLDPGVFIEYLVYSSIYLSFAAGAMAFISSVLH
                     NVAFNPVVLALGMLITFSVYNLNRKTDESEDAINHSHRFAFTKRYEHLLFASAIGAYV
                     LALVLSGLYGLSVLLISAIPLISGLIYSTPIFPKGFKYRRLKEIPLAKSMIVAIAWAL
                     PPALLPVYIGGAVPSLITLAVVLFFFSLVFINTVLFDIRDVEGDRATGVRTIPVCIGV
                     TNTKILLTLINIIFGVGIIVLLEFQIPMLFLCLFLFGMIYAQAYILLYEKISTGNLKC
                     DLIADGQFIVLGVLMVGIVFIQNSIQILLV"
     gene            complement(2303..3190)
                     /locus_tag="PK154_00370"
     CDS             complement(2303..3190)
                     /locus_tag="PK154_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HPW09547.1"
                     /translation="MFEQSRTMYSSNIAFTGLPPNHGLRAKEVDQYTRIMGDIQALFR
                     SRLQIQILLALADGTKTLSQLRDITGSSSQALIPKIRKLESSNFMAISDYEYHLTPVG
                     KILTKKIKDIILFKSVTKKHNNFWDQHYSEAIPEDFFVNLGELYNSDIILDTNVDIFN
                     VYFNFLRMIQDSKEIYILSPISSPAHTDAVLKRIREGVYVETLIGKDLIGQILQSPHF
                     DKIHELASHTTSKIFILDEMPRLGLTVTDKQISLGLYKSDGVTYDTTTDLFSADPAAV
                     TWGQKLYQYYKSNAVEFTL"
     gene            complement(3516..4325)
                     /locus_tag="PK154_00375"
     CDS             complement(3516..4325)
                     /locus_tag="PK154_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HPW09548.1"
                     /translation="MAVDEFTRMMGDIQALFRSRLQIQILIALMDGTKTLAQLREITG
                     SSSQALIPKIRKLETSNFITIANYQYNLTPIGRIFTKKIQDIILFKSVIKKHKKFWDQ
                     HYMQSIPDEYFSSVGDLYNSEIVTDTNVEIFNVYFNFLKIIQESEEIYILSPISSPAH
                     IDAVLKRINEGAYVETLVGKDLIAHLSQSPLFDKIYELASHTKSKILVLEEMPKLGLT
                     VTENHISLGLFKNDGITYDTTTDLYSTDPRAVNWGHMVYHYYRNMATELKY"
     gene            complement(4557..4949)
                     /locus_tag="PK154_00380"
     CDS             complement(4557..4949)
                     /locus_tag="PK154_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HPW09549.1"
                     /translation="MRWMALEMGKINDGVVADRRRLSDLVRELHPASVTRGGREYAFD
                     RNTIRVLWERLPEPLRYRLKLPILFYFDSTVTDSFMLADATALEALQALGELSDLREF
                     IGGRLWVGRAIVFAIMGKYPGAIQIMVS"
     gene            5178..5741
                     /locus_tag="PK154_00385"
     CDS             5178..5741
                     /locus_tag="PK154_00385"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HPW09550.1"
                     /translation="MTIPLAKNLLITGSPGIGKTTILVWLARALSDHRLAGFVTHEIR
                     ENGIRQGFLLVSCSGKKRILAHTDNHSPIRVGKYRIDLEGFECFLEEIHLEEKDPNLV
                     IIDEIGKMECFSPRFRELVLSLLASERPVIATIAKRGIPFMETIKMRPDVKLIEITMQ
                     SRDSIRDDLLNSAILMLDNHAGSGLGQ"
     gene            complement(6374..6447)
                     /locus_tag="PK154_00390"
     tRNA            complement(6374..6447)
                     /locus_tag="PK154_00390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6411..6413),aa:Val,seq:gac)
     gene            complement(6661..8262)
                     /gene="serA"
                     /locus_tag="PK154_00395"
     CDS             complement(6661..8262)
                     /gene="serA"
                     /locus_tag="PK154_00395"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPW09551.1"
                     /translation="MSFRILVSDPLAEDGLELLRSEFQVEVKTDLPEDELIRIIGDYD
                     ALLVRSGTEVTAKVIEAGKKLRFIGRAGAGVDNIDMDAATRRGIIVANAPEGNTLAAT
                     EHTMAMMLSLARNIPQANASLKKKEWKRSKFMGVELNEKTLGIVGLGRIGREIAKRAN
                     AMGMHCLGYDPFISKERAVQLGVESASLDELFRASDFITVHTPLIKETRHIINAKSIA
                     TMKDGVRIINCARGGIIDEKALYDAVKSGKVAGAALDVFEEEPPLNSALLTLDQVITT
                     PHLGASTVEAQTNVAVSIAKQCGAVLKGGSAKYIVNAPIVPADVADTIEPFAALAEKM
                     ARFLMQLVDGRVERVEFTYSGELSQLGTNTRFVTRMALKGLLDPILQVPVNIVNAEFI
                     AKERGISYAETTTPESGGFKSLVTIKVRTDMMEETMSGTVFFKGRSRIVSIGGYTMDM
                     IPEGYVIVSRHLDKPGVIGRASTILGKANINIAGMQVGRITPGEEAIMVLNVDGEVSP
                     EIMDEFRAKVGIFTAKFAKISDNLI"
     gene            8401..10674
                     /gene="ppsA"
                     /locus_tag="PK154_00400"
     CDS             8401..10674
                     /gene="ppsA"
                     /locus_tag="PK154_00400"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPW09552.1"
                     /translation="MKEMPDILWLEEIRKEDIAAVGGKGASLGEMSSMGLPVPGGFVV
                     TSRAFRHFLIETGIEQPLFSNLENLNVDDNNALEQASKKARALVMKAKMPASLKERIR
                     ESYQKLNGGGMVVAVRSSATAEDLPDASFAGQQETFLNIKGEKNLLDAVQKCWASLYG
                     ARAIYYRAKQGFDDRSVNIAVVVQQMINSEKAGVMFTSHPVTGEPLTIIEGSWGLGEA
                     VVSGSVSPDKYVYDQRTENVIDKLIAQKKTEIVSDGEHGTKTLEIGKERQETPVLSDD
                     EVARLATYGKVAETHYGIPQDVEWSIVGETIYILQSRPITTIRSGTTSVKTETGEGKI
                     LVKGQGASPGMATGRVVVIRDVKDTGSVKAGDILVTKMTNPDMVPAMQKVAAIVTDEG
                     GMTCHAAIVSRELGTPAVVGTRNATSLLKPGQLVTVDGEKGFIYEGALETPAQAQAPA
                     AAITASPLITATSVKVNVSIPGAAARAAATGADGVGLLRIEHLILGLNKTPGWFIANG
                     RDEEFIDELYKGIKIVLDAFNGKPVWVRTLDAPTDEFRNMAGGENEPHEHNPMLGWRG
                     IRRDLQSPEQFRLQVEAFKRLWEKGYDNLGVMFPMVSHPEEFIAAREMMAEWGVDVNA
                     ATLGVMIEIPSCAIMIEDFIHAGIDFASFGTNDLVQYTLAIDRNNANVASMYQPKHPA
                     VLALIDQAIEVCRDHGVECSICGQAGSEPPMVTWLVEHGITSVSANIDAIAKIRETVA
                     RTEQRIILESARRSHAE"
     gene            10664..11767
                     /gene="mfnA"
                     /locus_tag="PK154_00405"
     CDS             10664..11767
                     /gene="mfnA"
                     /locus_tag="PK154_00405"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HPW09553.1"
                     /translation="MRSKGIPEEDLFSFLQTTKLQDKNHRFILSSMCTLPHPVAVRAH
                     ALFMETNLGDPGLFSGTASLERTLISRIGTLFHHPDACGYATTGGTESNIQALRIARA
                     TAGIASPNVVIPESAHFSFKKACDMIGVEMRSVPLTGDYRVDCERYLESVDANTCCMV
                     GIAGTTEYGMVDPIRRLAAYARKEGIFFHVDAAFGGMVLPFLEGAPEFDFSLPGVMSI
                     AVDPHKMGMSTIPAGCLLLRNEKDLCSLSVDTPYLTVKQEFTLSGTRPGGSVAGALAV
                     LEYLGYEGMAAVVDGCMKNTRRLINGMESFGIRPVVIPDVNVATFEAPESPVPSPWQV
                     SYTRNNHLRIVCMPHVHAGIIEAFLEAIGEQYA"
     gene            11760..12308
                     /gene="hpt"
                     /locus_tag="PK154_00410"
     CDS             11760..12308
                     /gene="hpt"
                     /locus_tag="PK154_00410"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HPW09554.1"
                     /translation="MLEKLIKSLEICPVVKRGEYNYFIHPITDGVPSLDPAVLREVAT
                     GMIKIMDLDRVDRIVAAEAMGIPIGSIISHMTDLPLNIVRKREYQLRGEIPVHQVTGY
                     SKGQLFLNGVREGERVVIVDDVVSTGGTTRGILAALHVAGAEVADICFAIRKGYPDIG
                     RPYKVLVTVEVSDRVRVVEQHL"
     gene            12286..13233
                     /gene="dph2"
                     /locus_tag="PK154_00415"
     CDS             12286..13233
                     /gene="dph2"
                     /locus_tag="PK154_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HPW09555.1"
                     /translation="MWLNNISDLVTWLQEKRARSVALQFPAGLKRTAAGIADALSRQG
                     FSVIISGDPCYGACDLSLELLDYADGLVNFGHTPLLIHPDVLHIPYRVDFDPVVIRSA
                     LPFLKGKRIGLITTAQHTHLLPEIAEVLRNEGYDPIFQCAGGRTPEPGQVLGCSFAAA
                     RIPGVSEILFIGTGMFHPLGVQLATRAHVVALDPFSGQAVEVDAGKFLRKRHALIEKA
                     REAAHIGIIISTKQGQNRCDLGTRLLSLSDRAYLVFMKEVSPDELLNLGFEAYVNTAC
                     PRLAYDDQVRFPVPVLTPAEYEILCGTRTWEDYAIDEML"
     gene            13236..13856
                     /locus_tag="PK154_00420"
     CDS             13236..13856
                     /locus_tag="PK154_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HPW09556.1"
                     /translation="MKLKHLEKLLENLEGFSNPSPLLEQYRTPAPLAARLLFHAAMRG
                     DIEGKRVCDLGCGTGVLGIGAALLGSRDVVAVEIDPDVLGIARKNARQAAVEIHLIEA
                     EVKYPQVLPLVGACDTVVMNPPFGAQQIHADRPFIDAALAIAPVTYGIFNAGSRSFIS
                     SYIAGRAEIDEVIGGLLPIRRTFAFHREEVREIPVEIYRIVLHHET"
     gene            14301..15266
                     /gene="rfbB"
                     /locus_tag="PK154_00425"
     CDS             14301..15266
                     /gene="rfbB"
                     /locus_tag="PK154_00425"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPW09557.1"
                     /translation="MTRMLVTGGAGFIGSNFIRFLLNDRSDTEIVVNLDKLTYAGNLE
                     NLKDIAGNPAYRFIRGDICDAELVSRILDEFDIDTVVHFAAESHVDRSISEGLSFVTT
                     NVTGTCSLLEAARKAGISRFIHVSTDEVYGSIRKGSFSETDPLNPSSPYSASKAGSDL
                     LALSYFTTHHVPVIVTRCTNNFGPFQYPEKLIPLFTTNLIEGKKVPVYGSGMNVRDWI
                     YVLDHCRAIACILKKGIPGEIYNIGGGTELTNLEITRKILEQLGKDETSIQFVGDRKG
                     HDFRYSLSSDKLHSLGWQPRFTFDEALEHTIRWYKEQEWWWRKLK"
     gene            15248..16090
                     /gene="rfbD"
                     /locus_tag="PK154_00430"
     CDS             15248..16090
                     /gene="rfbD"
                     /locus_tag="PK154_00430"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPW09558.1"
                     /translation="MAKIKVTMQQNGHRTIILGANGMLGHAFQVVCPQALFCGREVDI
                     TDEAGIRSLFRRLQPSLVINAAAYTDVDGCEDHPDHAFLVNGEGPGYIAGACRKTGAS
                     LVHFSTDYLFDGDKEGYLESDPPHPINTYGQSKLLGEERIRETFDDYRIVRTSWLFGP
                     DGRNFVDTMLRLSKEQEIVRVVDDQVGRPTYTRDLAAKVLLLADEAPGIYHVTNEGTC
                     SWFEFARHIIPNVLPCTSDEIHRKAKRPRCSVLLNTKTAPLRHWKEALAEYLALLGRE
                     PRER"
     gene            16386..17489
                     /locus_tag="PK154_00435"
     CDS             16386..17489
                     /locus_tag="PK154_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW09559.1"
                     /translation="MKIIQTPVRIGTTGGVEEYVHHLSKGLTSRGHQVTVVCAAAGES
                     ALKDAGYPRKLLRSWFRLANTDITPALLPTLLSSDFDLIHTHIPTPWTAEISALVSRI
                     RNKPLILTYHNDIRGEGIYALVAWTYNRFILPLVLRQASRIIITRTRHLSPYLAPFSG
                     KLVCIPPGVDSHFFSPGKDPGKRGDLFFLGIFDEYHQYKGFDLLLQAIALLVQQGTDL
                     TLIAGGSGPLVGRYRELAADLSIERNVIFPGYIPGGELPSYYAGTRAFVLPSTDPSRE
                     GFGIVLLEAMACGRPVIATDIAGMAAEIEECGAGIIVKRNDPSELADAIRRLIDNKPL
                     AGRMGDAGRRLIEERFGWEIIAGEVETLYAGVL"
     gene            17473..18528
                     /locus_tag="PK154_00440"
     CDS             17473..18528
                     /locus_tag="PK154_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW09560.1"
                     /translation="MPEYSESETVAFAGLEQREIPYSGNSEKMNDHPLVTVVVVNYNG
                     RHFLRECLASITNQTYAPLEVILVDNASSDGSVEFVRALFPGIHIVQNNENRGFSGGV
                     NSGIRESNGTYIITLNNDTRMDSHCIRYLVHAMSGDDRIGMGVIKMLFPDGTINSTGI
                     CISRSGAAWDRDMFKRDSPENSPGGEIVGPCAGAAMYRRSMLDAIGLFDEDFFLFMED
                     VDLALRARLAGWECAYVPEAVVYHHHGGTAGFMSDLTVYYGNRNILWYPVKDFPAGYL
                     VTSIPWIMGRTIAVIPYYAVRGQGRVILKSKIDGMKGIPDMIRKRRTVERIITPGIVK
                     KIIRTWSSVQRRSDVDH"
     gene            18515..19276
                     /locus_tag="PK154_00445"
     CDS             18515..19276
                     /locus_tag="PK154_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW09561.1"
                     /translation="MWITEYRDLISILAISDLRVKYQSSVLGFAWSLVNPLLMMLVLY
                     IVFSNVFHMSENQYALYILIGIVSWRFLQNGSSAAMTSIVGKPGLVTKVYIPRQVLVL
                     SSVLSGFVSSLLEFLVLICLLVAFSVPISFVVLLFPVIHLLFLFLLYGLSLSLASLYV
                     YYRDLNQIWEVLLQAGFFLCPIVYPLSTIPEQYLPFYMLNPITDFIAIYRDIFLYGKL
                     PSPASIAIIVIAGIILYLIGRLLFGKLERRFAEEV"
     gene            19278..20063
                     /locus_tag="PK154_00450"
     CDS             19278..20063
                     /locus_tag="PK154_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09562.1"
                     /translation="MEEITRDTIDEGDAIIVEHLSKRYRIPHERKQTIFEHLFSIFKG
                     GAYSYEEFCALHDVSFRVRHGETYGIIGPNGSGKSTLLKILAGVLYADGGVKIVQGRI
                     APFIELGVGFQPDLTAKENIYLYGAIMGLKKREIRERYDEIIEFAELKRFENMRLKNF
                     SSGMVVRLAFSTAIQTDPDILLVDEVLSVGDESFQQKCNQKIGELRSKGKTIIFVSHS
                     LETVHTLCSRCLLLDKGQVIQIGPTPDVVHTYLRLIGSKGALS"
     gene            20214..22382
                     /locus_tag="PK154_00455"
     CDS             20214..22382
                     /locus_tag="PK154_00455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09563.1"
                     /translation="MDPDILVIFEQKLHLFIIDPLLGFDTPQRKMYDLGEKSVHILLE
                     EGLWGLVDSFRERRRVQRLARRVTREYAEIPSPRPWSGEELVFPQRPDNPEVSIVIPV
                     YNNLTFTFNCLRSIVEHTTGSYEIIVVDDASSDDTPRFCSLQNELIIVRNETNKGFVG
                     SCNRGAEMASGQYILFLNNDTQVTGGWLEPLIRTMQRDQVGAVGSKLVYPDGRLQEAG
                     GIVFNDASGWNYGRGDDPDRPEYNFLREVDYCSGAALMVRTDLFRHLGGFDEWFTPGY
                     YEDTDLCFRIRQEGYRVLYQPKSAVIHYEGMTAGREGAGGMKEFQQVNRDKFFSRWSA
                     VLARSHKPPDSRNLFHTRIRRAGQNVLIIDHYLPTFDRDSGSLRMYYLLEILSRLGYH
                     VTFVGDNPSPLEPYCSLVQDLGVEVLYTPYVYSIEKYLKEHGKNFDVVLLSRAHIAWK
                     YIYHIRRFCQSARILFDTVDLQFVREARRYEIEGGKKLLASVRKMKEMELRIARLSDT
                     TLVVSPVEKEILLKENPSLHVEVLSNIHHIFPVSKGFFERRDLLFIGGFIHPPNVDAV
                     VWFTDEIFPLVQREIPEVGMYVVGDDPPPHFRAFDSDAVKVTGYVQDVTSFFSDCRVF
                     VAPLRYGAGIKGKINQSMSYGLPVVTTTIGAEGMGLIHGENALIADDPGDFADEVIRL
                     YRDEALWNRISSGSQENVKKFFSYDTASEALLKILKSSSE"
     gene            23076..24821
                     /locus_tag="PK154_00460"
     CDS             23076..24821
                     /locus_tag="PK154_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPW09564.1"
                     /translation="MRIALHSQYLDDWDSIQFVLALKEYSIPAHQPHPPGYPIYVLLG
                     KIANAVIRSGQHSFIVISATFGSLSLIPTYYLGKYLFNEKIGILAAFILSLTPAALLF
                     SEVVMSDIVSMFFIVLTLCLLYVGIENDICLYIGSFILGITIGVRQTDFLLLPLFFYI
                     LYSKGNIKKSILSSFIAIFAILIWLVPVLIITGPEAFLNAQRSQGNVVFGYGIWNTLS
                     WPILLNFLHSILRLLILLIKGWSLSLFLFVAVTIIIVVYPYLKNYRFDLDKRKLFFVL
                     WILPYLAYFILFGQLLLYIPRYLLPIFPAFALIFSYSLVKAINISNKKIMKTFLIVIF
                     AISIGFMGYQAVSTVYRLHTTMPAPVQAANFIKEHYNPENTVIIGDYESFRHYYYYLS
                     EYDVRYIGVMNTTEAYYLLLENKTVVSGIDLGPSPKEIHLFSRDPKIYPKHHDVRLFI
                     FQLAENRENFYFGNGWYTREMRDAIPFNWIGTNASLYIYSEENREVDLNFQAISAIRP
                     KVLDVYEGTHPIATFTISHEQLTDVRLPLTLSKGTTIVFFSLPEGCERPSDFQEVKNP
                     DSRCLGIAIQNITFI"
     gene            25046..27358
                     /locus_tag="PK154_00465"
     CDS             25046..27358
                     /locus_tag="PK154_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09565.1"
                     /translation="MPDPTNKRELILFSLKSCEAGEGTAYVKEYFFTSLIADGRSILD
                     LGGGNRGGTVALGEVAQTVTGITREKHSFLVDRVEPSPNVTFLAGDSRALPFPDKTWD
                     IVLLFSGESIKGSFDAVLAEVSRVLKDDGIFITTVQAMDSAYGKPGGEGKYPLSEDSP
                     SWRASIERVFPFIVGFSQTGDGGCGMVPLFPAQRGEILENIPDKQGPDAPCLIVAARR
                     PVTLPWLEGMTWAAAGRSDQPVFEREHEKRIAFLESVIEQRDLALQVARERNEKLKEE
                     VVEMKRSVLWRCAMTYHTAIVEPLLGHATRRRRWYDAGLSGLRLVSNEGVSGLLWHVR
                     GGAARNRHSSFSRLFHRSADRSTRSIRERISLIIPTKNAGHEFRYLLRKIRTQKGVSD
                     VEIIVIDSGSSDETVAIARSFDCHVIEISPEQFHHGGTRNAAASHATGEYISFTVQDA
                     LPCGEFWLRDLVLPLAEDTSVSAATARQVPRSDADLFACFNIWANYQAIDFLRDKVVS
                     APPDYSSLSLMQWRKLAQIDDVCSAFRARIFSEFRFLTDYAEDLDIGVRLLKAGHKVA
                     FVHSSAVIHSHNRTAAYFFQRSYADSFTLSTVFPHDGSFIPENRSPEEILGGIFSLYQ
                     TFNAAVKILKEQSIAELSEEMTQQIKYLMKSELSRPDTADGEISPGEPSMDSLFYLLQ
                     ERLPFRGPFHQDTRLLRLFFNNLDTFRDFRKRCGNPPLDPVEIEAALYKLFAAASGIS
                     LGGYLALLKEGNSVHTTSLETLHASLTEGI"
     gene            27358..28635
                     /locus_tag="PK154_00470"
     CDS             27358..28635
                     /locus_tag="PK154_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW09566.1"
                     /translation="MKVLFAVHQFFPHHYTGTERVVLNLCRQLQRMGHDVIVLTYAID
                     SAEGLYPRGAFLVGKYSFKGIPVIAVRHREPPEDLDFIFRDEGMQALLESIIEDEQVD
                     IIHVCHPMRVGSIIPAAISRSLPVVVTLTDFWLLCPKFIAVTGKGIPCDGPWGGKRCA
                     EECYEPSWKYRIRERLQEIDGVKNQVSAFVSPTDFLKRIFEGNRYRSVYRIPFGLDYQ
                     DIRIQEKKYNDDSAVVFGYLSTPVRHKGAHVLIDAFLRADMPNTSLKVFGDDRADREY
                     YENLKSASGGDPRVEFCGRYHHEELSSILGSLDVVVIPSLWWENSPLVLLTALACGVP
                     AIVSDLGGLTESVQDGVNGYSFKAGDIDALATVIRTIASSPSGLQELRAGITYPARIE
                     EEAVQYEKIYQNIWNPGEKHGSAGKKRQRSRVP"
     gene            28685..29608
                     /locus_tag="PK154_00475"
     CDS             28685..29608
                     /locus_tag="PK154_00475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW09567.1"
                     /translation="MKRCLVLGGNGFLGSHLAEALVVSGFEVTIFDSREQKRPDIRED
                     SIGYRQGDFLNENDLARALAGTDYVFHFISTTLPGPSLESPVYDIESNVIGTVRLLQQ
                     CCKAGVEKIIFPSSGGTVYGEPDTIPVRETDPVRPVHPYAISKMTIEMYLRSFYELHG
                     LDYLILRYANPYGERQMPFGNQGVIPIFLHAIVKGRSPVIYGDGSMVRDYIYISDLVD
                     ATIAIVKKDPPERLFNVGSGRGTSVHELLGTMAEVLGVEINPLYADPPGTYLSRIILD
                     ISRMQHLTGWKPRVSLAEGIERTYRWLKSLP"
     gene            complement(29765..31543)
                     /locus_tag="PK154_00480"
     CDS             complement(29765..31543)
                     /locus_tag="PK154_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPW09568.1"
                     /translation="MKPSQVGTLILFLFICLFIAVPAATASDERAIPSVRADSEVWAV
                     IVGISDFRFVNDLSYCDDDARDLTTYLQNDCAIPADHIVTLIDSQASKNAIQSKIAEL
                     QSKSGTDDIVVVSFSSHGTYASDLSPYDESDGYDEYIIAWDTRFLSGAIRDDELKAWL
                     DAVPSKNTLLIVDTCFSGGMTKDTGSRFISLQDTTTTPPSTRDGLRQDFSRDLSDSRY
                     TVLMACNDAELSGESSALQNGYFTYFLLEGLRSLSADTNSNTYISAEEGFFYAKPKVA
                     AYSSNSQNPQIYDGDTAHQIELTLPQGAPAPTVTGVTPGSGVVGSSVSISNLAGTNFQ
                     GGASVELQNDGQVIEASGELVVTASQITCTIDIPSDAAVGSWDVVVTNPDTQYGILSN
                     GFSVTSGGLPAPTVTAITPNTGAAGTTVSITNLAGSSFQSGAAVELRKDGQVIAATSE
                     GVVSDTQITCTIVLPSDATIGSWDVVVINPDTQSGTLAGGFTVTAGSTPAPTVSRIQP
                     SSGRLGTTVTVTNLAGTNFMPGAQVQLRKSGAPTIEATNEVVVSSLKITGKIPIPSTA
                     TKGVYDVVVINPDGKSGIKSSAFRIL"
     gene            complement(31597..32469)
                     /locus_tag="PK154_00485"
     CDS             complement(31597..32469)
                     /locus_tag="PK154_00485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW09569.1"
                     /translation="MRAYFRRIFGKNPPVQELPKEEQNEHDTNSIPLPEGKDLLTEEL
                     LGFCRDEAASFQVVPDIHPDDFIFQFVLTNPYFTNPKDAVHYYFNDGNNSARRLSSIL
                     TNVLHLEHGPIQILEFASGYGCVTRHLPRMVPVAHVTACDIHEQATGFIQQTMGIDTV
                     LSHSVPEEVRFPREYDVIFALSFFSHMPRSSWGRWIQTLYRSVAPSGYLIFTTHGEES
                     LKYQPEAVMPPEGFLFKPTSEQKDLASEEYGTTVTLPDWVIPEVYRMTQAPLVCYKKA
                     YWWGHQDLYILKKI"
     gene            complement(32529..33743)
                     /locus_tag="PK154_00490"
     CDS             complement(32529..33743)
                     /locus_tag="PK154_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09570.1"
                     /translation="MVNRIKDIFVFVIITGLLIVPVVSGEDPADLEVVLTADGGDLTQ
                     TAYNAVATTGGLVATVSMISMQGAAPGTVDQSALNSVAVTGDDQEVIMEIEMTAIAGG
                     DISQDATNTAAIAGDNVDLSQSISMSADSAEGTVTQNAENYAIFEGNQAFIEQYISMS
                     GIGSQVFQNPDSQGNVLFYMGDEGIVSQYFEAAASGLDLVEQYLQNYVDSTGDDSVIG
                     QSIIASAVSGNGPVYQVGMNVANGDDGMDLGQALWLAGSSPDVWQESENVARFQNDAT
                     ISQWIALSGISTENDVTQYAYNSAFGTDDLGDSTSFVAQGISGTAWGRPTATQLFSNY
                     IITSRDPDESLIAAQSITATINAEQYAGQELGNYMDWWGGVDGQLGQEILASSFADLI
                     SQYSGNYLNPFP"
     gene            complement(33920..>34532)
                     /locus_tag="PK154_00495"
     CDS             complement(33920..>34532)
                     /locus_tag="PK154_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09571.1"
                     /translation="YNEIDYGTDVGITWGNEIASQSISLGGVASDEVYQYGWNWADLD
                     DNAVLGQLVSIGGDGSYVDQEAYNDAYFGYSATIAQALEFSAISDDEGVDQYGYNYAT
                     NPWDDGGATSAIGQSISAAATGGDISYQEFYNTAALDWNWAPGTYNVVQSNVATLAAT
                     DEAYQGMYNWVYWWDPNTGSVAMDILGDASADYVYQYHENWIG"
BASE COUNT         8422 a         8529 c         8937 g         8644 t
ORIGIN      
        1 aagaaggaac atcaatgcaa ctatcccggt cactccaccg gcaatccctg ctgttggtgt
       61 gggggagagt tcagtgctgg ttacttccgc tgtaagggtt gcagtgtcat ttatcagggc
      121 aaccgttgga agttcagtgg ggacgggggt acctgattcc gtgacggaag cttcggtctc
      181 catggtctct cgggattggt cgccggcata tgccatagag agcttttcgg cgacatcttc
      241 aagattcgga ttcatggcga gggccttaag atactgctcc acggcatcat catatgctcc
      301 tgcagcgaaa cgggcatcac caagaagagc atagacgctg gcatcaccgg gatcgagttt
      361 tagcgcctta ccaaacgcat tttctgcttc aacggtcctg ttcagggaca gaagtgcatt
      421 tccctttcct ttccatgccc tgaacgagaa cggatcagag gatagtgcct tttcgtaggc
      481 ggcgagtgat tcgttatact ggcctttcgt gaagagttca tcacctttta tccgccagag
      541 gactccttcc tcagcagatt ctccacttgc tccggatgaa gagccggaat ctgatgagga
      601 actgtctgaa ccggagctgc cactggtact ttcctgccca tcacttgggt cactgccacc
      661 tgatcctgac ccactatcac cagaactgct gtcatccccg gaactcccgg aatcaccaga
      721 actgctgcta tccccggaac tcccggaatc accagaactg ctgctatctc cggaactccc
      781 ggaatcacca gaactgctgc tatctccgga actcccggag tcatcatcgt catcgaagct
      841 tcccgacccg ccatcatccc aatcatctga tggagaatcc caggatcccc cgcaaccaca
      901 gtcatctgca gctgcgctcc ccacgatgac cagaataatg agagcggcta caatgagaga
      961 tcgcactggc ttcataagga gaattaggcc actaacaaat tataggttcc cccttgaggg
     1021 gaatagtgcc ttctgcatca gacgagcagg atctgtatcg aattctggat gaacacaata
     1081 ccaaccatca gcacaccaag gacaatgaat tgcccgtctg caatcaggtc acacttcaga
     1141 tttcctgtgc ttatcttttc gtataacagg atgtatgcct gggcatagat cattccgaag
     1201 agaaacaggc agagaaacag catcggaatc tggaattcga gcaacacgat tatccccaca
     1261 ccaaagatga tattgatcag agtaaggagg atctttgtat tggtcacccc gatgcagaca
     1321 ggtatggtcc gaacccccgt tgcacggtca ccctcaacat ccctgatatc aaacaggacc
     1381 gtgttgataa aaaccagcga gaagaagaac aataccactg caagcgtaat aaggctcggt
     1441 actgcccctc cgatgtaaac gggcaacagc gcaggcggaa gtgcccaggc aatggctact
     1501 atcatacttt ttgcgagagg aatttctttc agtctcctgt atttgaatcc cttagggaag
     1561 atgggtgtgc tgtaaatcaa ccctgatatg aggggtattg ccgagataag aagcacactc
     1621 aatccataaa gaccagaaag gacgagggca agcacatatg cgccgattgc tgaagcgaag
     1681 agcagatgtt catacctctt tgtgaaggca aaccggtgag aatgattgat ggcatcctcg
     1741 ctctcatcgg ttttcctgtt caggttgtat accgaaaagg tgatcagcat cccaagtgca
     1801 agcaccacgg ggttgaaagc gacattatgc aggacactgg agatgaaggc cattgccccc
     1861 gcagcaaaag agaggtagat tgagctataa accaggtact cgatgaaaac accgggatcc
     1921 aggtcaaaag tgtataccgg atggctatgt tcaaacatag attattcttc atgactagaa
     1981 taaacataca ttcccatggt gaaaagtacg cgaaaaggtt aatattaatc tgaacgatta
     2041 atcaaattaa aagaatagcg ccagccccag cctggggata aaccaaagat attgtgtaat
     2101 tatccctttt ccgatatgag atatatattt ttgcccggca aaggctcttt tttggggaga
     2161 ttgggctcaa ataactgaaa aaacacatgg aaacatacat cataactcaa tttcagacct
     2221 cgtgatcgtg aaatgccacc cgaatgcaac tgatgcaaaa tgttcccggg aaaagatatc
     2281 caggactatt attgagaaat gattataagg taaattccac tgcattactt ttataatact
     2341 gatacagctt ctgcccccac gtaacggctg ctggatctgc tgagaacaaa tcggtggtgg
     2401 tgtcataggt gactccatca ctcttgtaaa gaccaagcga gatctgctta tcggtgactg
     2461 tcaatcccag cctgggcatt tcatcgagaa tgaaaatctt gctcgtggta tggcttgcaa
     2521 gttcgtgaat tttatcgaaa tggggagatt gtaggatctg tccgataagg tcttttccta
     2581 tcagtgtttc cacatataca ccttccctga ttcgtttgag tacagcatcg gtatgggctg
     2641 ggcttgagat aggggagagg atgtagatct cttttgaatc ctgtatcatc ctgagaaaat
     2701 tgaagtacac attgaagata tcaacattcg tatcaagaat gatatcggaa ttgtacaatt
     2761 ctccgagatt cacgaaaaaa tcttcaggga ttgcttctga gtaatgctga tcccagaaat
     2821 tgttgtgctt ttttgtgact gatttgaaaa ggataatatc cttgatcttt tttgtgagga
     2881 tctttcccac gggagtcagg tggtattcgt agtcagagat tgccataaaa ttacttgatt
     2941 ccagctttcg gatctttgga atcagtgcct gggatgagct tccggtaata tctctgagct
     3001 gcgaaagggt ttttgttcca tcagcaagtg caagaaggat ctggatctgg agtctcgacc
     3061 gaaagagggc ctggatatcc cccatgatgc gggtatactg atcaacttct tttgctctca
     3121 aaccatgatt tgggggcagt ccggtgaatg caatgttcga actatacatc gtccggctct
     3181 gttcaaacat aacttgttct tcgagggtga aataaacata aattcctatg gcgaagaatc
     3241 gccaaagaga tgaatgttaa tcgtagcagt taatgtccgt gcttaaaagg gctattcccg
     3301 tcatagggaa cagaggagct gaaatatccc ttcctatacg atataacttt gcaaggcatt
     3361 gaacaatcag aacactgtca atctcatatc ccgcgggtag tttcgcgaaa agggatgaga
     3421 tgtacatgaa cttattgaaa aatgcgctat caggccacat attctttgca agggatattc
     3481 ggacaaatga aagatcgggt tgccaagatg aaccatcagt atttcaattc ggttgccata
     3541 ttccggtaat agtgatacac catatgcccc cagttgacag ctcttggatc tgtggaatac
     3601 aggtcggttg tggtatcata cgtgatgccg tcattcttga aaagcccgag tgagatatgg
     3661 ttctctgtaa ccgtaagtcc cagttttggc atctcttcca gaacgagtat tttacttttc
     3721 gtatgactgg ctagttcata gattttatcg aacagtggtg attgcgagag gtgtgcgata
     3781 aggtcttttc ccaccagtgt ttccacatag gcaccttcat taatccgttt cagaactgca
     3841 tcaatgtggg cagggcttga gataggtgaa aggatataga tctcttccga ttcctgtata
     3901 atcttaagaa aattgaaata aacattgaat atttcgacat tggtatcggt gacaatttct
     3961 gaattgtaca ggtcaccgac agaagagaaa tactcatctg gtatcgactg catgtaatgc
     4021 tggtcccaga attttttatg tttcttgatc actgatttga aaaggatgat atcctggatt
     4081 tttttggtga agatcctccc tatgggggtt aggttgtact ggtaattggc tatcgttatg
     4141 aaattacttg tctcaagttt tcgtatcttc gggatgagag cctgcgagga acttcctgtt
     4201 atttcacgga gttgtgcaag agtctttgta ccatccatga gggctattaa aatctggatc
     4261 tgaagtcgcg agcggaaaag tgcctggata tctcccatca tacgagtgaa ctcgtctact
     4321 gccattctgt gtcacccaca ctgacctgcc tgccggaaaa tgtcatttca gggataacca
     4381 ccgcaatgcg tgttcccccg tttctgatgg cgaatgacaa attctcatca gtgccattaa
     4441 tcaatgagat taatcttcat ctatacgatt aaccttcctt aaggtaccat gtaatcgtat
     4501 ataataccta tcgaatcact tgtccggcgc tcccattcag ggttcaaaga taccgatcaa
     4561 ctgaccatga tctggatggc accaggatac ttgcccataa tggcaaacac gatcgccctg
     4621 cccacccata acctcccccc gatgaattcc ctcaggtcag acaactcccc aagagcttgt
     4681 aacgcctcaa gtgccgtagc atcagccagc atgaagctgt cagtgaccgt tgaatcaaag
     4741 taaaaaagaa tggggagttt gaggcggtag cggagaggtt caggaagtct ctcccagaga
     4801 acccggatcg tatttctatc aaaagcatac tctctaccac cacgggtgac cgatgcagga
     4861 tgtagttccc tgacaaggtc tgaaagtctc cttcgatcag caacgacgcc atcatttatt
     4921 ttccccatct cgagagccat ccatctcatg agaacggatt catctgacac gggtgggcca
     4981 aaggtcatac tcatttagta ttctggtttt tttggtaagt aatctattcc caccggcaga
     5041 attttttacg taaacagcaa cagcacatta cccggttcaa cctccttgac acaacaagga
     5101 gaagtctgcc agagacgata tctggtgaac agcaatggag ggacacctcg taaagaatcg
     5161 ggggatgacc cattatcatg accataccat tggcaaaaaa cctcctcatc accggcagtc
     5221 ccgggattgg caagaccacg atcctggtat ggctggcaag agccttgtca gatcatcgac
     5281 tcgccggctt tgtcacccat gagatcaggg agaatggtat caggcaggga ttccttcttg
     5341 tcagctgttc agggaagaag cgtattcttg cccatactga taaccatagc ccgattcggg
     5401 tgggaaagta tcgaatagac ctggaaggat tcgagtgttt tctcgaggag atacacctgg
     5461 aggaaaaaga tccgaatctt gtcattatcg atgaaatagg gaaaatggaa tgcttttcac
     5521 ctcgtttcag ggaactggtg ctctccctcc ttgcctccga acgccctgtt attgccacca
     5581 tagcgaagag aggtattccc ttcatggaaa cgatcaaaat gagacctgat gtgaaactga
     5641 ttgagataac aatgcaaagc cgggactcta tcagagatga tctcctgaat tcggcgattt
     5701 tgatgctgga taaccatgct ggatcaggac tgggacagta ggcaggtata cgtgaatccc
     5761 tcatgattca caaaatctct taaaaatcag gtccaccaga tgagaaagga ggatgaacag
     5821 aggattgatc agggagtgtg attacttcag gggaatggga atacttcgca tggatagcct
     5881 ttctgacaag gatcctgtca tctggctgtt tccaatgatc gggtgattta ccagcagccg
     5941 actgtttgag atatgatccg gttcacgatt tcccggggta tcgaaagttc tcctccctga
     6001 ttattcactg caatccaggt attgagggcc aaggcaacgg tcagtatctc acggggattg
     6061 tgggtggctt ttgtgatcca cctacagatc tcctctttat tggaaatggt aaggcagtat
     6121 gaatgctcaa tcatctgcag atgactcatt accatctcat ggttgtcagc agattgtggc
     6181 atggttcata cctgaatttc gctctataag taactacctc aatgatgaac cccaaggcct
     6241 ggatttgaat acttagagag acaagaggtt cccgatcttc gactcacttc tgggtattat
     6301 gtcgatgaga ttgcgatagg atgaaatcac gttccatgaa agtgccggat ccacgttcct
     6361 ggaaaaacag caatgggctc gaggagattc gaactcctga cctccgccat gtcaaggcga
     6421 cgtcataacc agctagacca cgagcccgct ctcttgcgag atgcctctta atataagtca
     6481 catgactatt aaactattct cttttcctcc aataaacgac tgtaaaccgg aaatcctatg
     6541 gtgaaccgga taatttcggt gtttgcagga acctatttct ctggaaaata actgatatgc
     6601 atgcggtata ccatcgtgtc ttatttttct cccttaaaag agaagggaaa aatgattttt
     6661 tcagatcagg ttatcggaaa tctttgcaaa ttttgcggta aaaattccga ctttcgcccg
     6721 gaactcgtcc atgatttcag gagaaacttc accatcgacg ttcaggacca tgattgcttc
     6781 ttcgccagga gtgatcctcc ctacctgcat gccggcaatg ttgatgttgg ccttgccgag
     6841 aattgttgac gccctgccga tcaccccggg cttgtcgagg tggcgggata cgatgacata
     6901 cccctcgggt atcatgtcca tggtatatcc gccgatggaa acgatccggc tccttccctt
     6961 gaaaaacacc gtgccgctca tcgtctcttc catcatgtcg gtcctgactt tgatggtgac
     7021 aaggctcttg aatccgccag attcaggagt ggtggtctcc gcatagctga ttccccgctc
     7081 ctttgcgatg aactcggcgt tcacgatatt gaccggaacc tggaggattg gatcgagaag
     7141 gcctttcagc gccatccttg ttacgaaccg tgtattggtt cccagctggg agagttcccc
     7201 gctgtaggtg aactccaccc gttccacccg tccatccacc agttgcatga ggaaccgtgc
     7261 catcttctca gcgagggctg caaacggctc gatggtatct gcgacatcgg cagggacgat
     7321 tggtgcgttc acaatatact ttgccgaacc tcctttcagg acggccccgc actgttttgc
     7381 gattgagaca gcaacattgg tctgggcttc aaccgtgctc gcaccgagat gtggggtcgt
     7441 gatgacctgg tcaagggtga gaagcgcgga gttgagcggc ggctcctctt cgaaaacatc
     7501 gagcgccgca ccagccactt tccctgattt cacggcatcg tagagtgcct tttcatcgat
     7561 gatgcccccg cgggcacaat tgatgatcct gacgccatcc ttcatcgtgg caatgctctt
     7621 tgcattgatg atgtgcctgg tctccttgat gaggggagta tgcacggtaa tgaaatccga
     7681 ggccctgaag agctcatcga gggacgcaga ttcgacaccc agctggacag cccgctcttt
     7741 gctgataaac ggatcatatc ccaggcagtg cattcccatg gcattggccc tttttgcgat
     7801 ctcccgtccg atcctgccaa gcccgactat cccgagcgtc ttctcattca gctccacccc
     7861 catgaacttc gagcgcttcc actccttctt cttcagggat gcattcgcct ggggaatgtt
     7921 ccgggcaagg gataacatca tggccatcgt gtgttcggtt gcagcaaggg tgtttccctc
     7981 gggggcattt gcaacaataa ttccccgccg ggtcgctgca tccatatcga tattatcgac
     8041 gccagctcct gcccggccga tgaaccggag cttttttccc gcctcgatta cctttgcggt
     8101 gacttcggtc ccactccgga caaggagcgc gtcgtaatcc ccgatgatcc tgatcagctc
     8161 atcttccggc agatcggttt tgacctctac ctggaactcc gatctgagca gctccaaacc
     8221 atcttcggcc agcggatcgc tgaccagtat cctgaagctc atatgaaaac ccatactata
     8281 tcgttaccag agcttttcta accttcttct caattcatag tcctgtcggt ccgctggccg
     8341 ggacatcagg agatgataat ttattaacca gatgacaggt gagattatag tggtgcgtga
     8401 atgaaggaga tgccggatat tctgtggctt gaagaaatac gaaaagaaga tatcgctgca
     8461 gtagggggaa aaggagcctc gcttggagag atgtcatcga tgggcctccc ggtaccgggg
     8521 ggatttgttg tgacgagccg ggcgttccgc cacttcctca ttgagacagg gatcgaacag
     8581 cccctgtttt ccaatcttga gaatttgaac gtcgatgata acaatgctct tgaacaagct
     8641 tcaaagaaag cccgtgcact ggtgatgaaa gcaaagatgc cagcatccct gaaagagagg
     8701 atccgggaat cctaccagaa gctgaacggt ggcgggatgg tggttgccgt caggtcaagt
     8761 gcgacggctg aggaccttcc cgatgcaagt ttcgccggcc agcaggagac cttcctgaat
     8821 atcaaaggtg agaagaacct ccttgatgct gtccagaagt gttgggcttc gctctatgga
     8881 gcgagggcaa tttactaccg tgcaaaacag ggttttgatg accggagtgt caacatcgct
     8941 gtggttgtcc agcagatgat caactcggag aaagcagggg tgatgttcac ttcccatccg
     9001 gtgacgggag aacctctgac gatcatcgag ggatcctggg gtctgggaga agcagtggtc
     9061 tccggctcgg tatctcccga caaatatgtc tatgaccagc ggaccgaaaa cgtgatcgac
     9121 aagctcattg cacagaagaa aaccgagatc gtctccgatg gtgaacatgg cacaaagact
     9181 cttgaaatag gaaaagaacg acaggagaca ccggttcttt ctgacgatga ggttgcacgg
     9241 ctggccacgt atgggaaggt cgcggaaacc cactacggga ttccacagga cgtcgaatgg
     9301 agcatagtcg gggaaaccat ctacatcctc cagtcccggc ccatcaccac catcaggtca
     9361 ggtacaactt cggtaaaaac agaaaccggc gagggaaaga tcctggtcaa aggccagggg
     9421 gcatcaccgg gcatggcgac cggccgtgtc gtggttatcc gcgacgtcaa agatacggga
     9481 tcggtaaagg caggagacat ccttgtcacg aaaatgacaa accctgacat ggtgcccgcg
     9541 atgcagaagg ttgcagcgat tgtgaccgat gaagggggga tgacctgcca tgccgcaatc
     9601 gtgagcaggg aactcggtac cccggcggtt gtcgggacaa gaaatgccac atccctactc
     9661 aaaccaggcc agctggtcac ggttgatggt gaaaaaggat ttatttacga aggggcactc
     9721 gaaacgccag cccaggcaca agcccctgcg gcggcgatca ctgcatcacc ccttatcacg
     9781 gcaaccagcg tgaaggtgaa cgtctctatc cccggagcgg cggctcgcgc tgctgcgaca
     9841 ggtgctgacg gggtgggtct cctccggatc gagcacctca tcctcgggct gaacaagacg
     9901 ccggggtggt ttatcgcgaa tggcagggat gaggaattca tcgatgagct gtacaaaggg
     9961 atcaagatcg tacttgatgc gttcaacgga aagccggtct gggtgcggac gcttgatgcc
    10021 ccgaccgacg agttccggaa tatggccggt ggcgagaatg aaccccatga gcacaacccg
    10081 atgctgggat ggagaggtat ccggcgcgat ctccagagcc ccgagcagtt ccgtctccag
    10141 gtcgaggcgt tcaaacggct ctgggaaaag ggatacgaca accttggtgt gatgtttccc
    10201 atggtatcgc acccggaaga gttcatcgct gcccgggaga tgatggcaga atggggagtg
    10261 gatgtcaacg ccgcgaccct tggggtgatg atcgagatcc cgtcatgtgc catcatgatc
    10321 gaggatttca tccatgccgg gatcgatttc gcctcgtttg ggaccaatga tctcgtccag
    10381 tacaccctgg ctattgatcg gaacaacgca aacgttgcct caatgtacca gccgaagcat
    10441 cccgcggtgc tggcgctgat cgaccaggcg atcgaggtct gccgtgacca cggggtcgaa
    10501 tgttccatct gcggccaggc tggttctgag ccccccatgg tgacctggct cgtagagcat
    10561 ggaataacga gtgtctcggc caacatcgat gccattgcaa agatacggga gaccgtcgca
    10621 cggacagagc agcgaatcat tcttgagagt gcaagaaggt cacatgcgga gtaaaggaat
    10681 ccctgaagaa gacctcttct catttttgca gacaacaaag ctacaggata aaaaccaccg
    10741 gttcatcctg agctcgatgt gtactctccc gcatcctgtc gcagtcaggg cgcatgccct
    10801 cttcatggag accaatctcg gagatccggg gcttttttcg ggaaccgctt cgcttgaacg
    10861 aacgcttatc agccgtatcg ggaccctctt ccaccacccg gatgcctgcg ggtatgccac
    10921 gaccggaggg actgaatcaa atatccaggc actcaggatt gcaagggcga ccgcagggat
    10981 tgcatcaccc aatgtggtca tacctgaatc cgcccacttc tcatttaaga aggcctgtga
    11041 tatgatcggg gttgagatgc ggtcggttcc cctcacgggg gattaccggg tcgattgtga
    11101 acggtacctg gagtccgttg atgcgaatac ctgctgcatg gtcggcattg caggaacaac
    11161 cgagtatggc atggtagatc ccatcaggag gcttgcagcc tatgcgcgga aggaggggat
    11221 attcttccat gtcgatgctg cgttcggggg tatggtcctt ccgttcctgg aaggggctcc
    11281 cgaatttgat ttctcgctcc ctggggtaat gtcgatcgct gtcgatcctc acaaaatggg
    11341 gatgagcacc ataccagccg gctgcctcct tttaaggaat gaaaaagatc tctgttcatt
    11401 atcagtagat accccctacc ttaccgtgaa gcaggagttt acgctctccg gcacccggcc
    11461 cggtggatcc gtcgccggag cactcgcggt gcttgaatat cttggctacg aggggatggc
    11521 agcggtggtc gatgggtgca tgaagaatac cagaaggttg atcaacggga tggagagctt
    11581 cggaatccgg ccggttgtca tccccgatgt caacgttgcc acctttgaag ccccggaatc
    11641 gccggttcct tcaccctggc aggtctcgta cacccggaac aaccatctca gaatcgtctg
    11701 catgccccat gtgcatgcgg ggattattga agcatttctt gaagctattg gtgagcagta
    11761 tgcttgaaaa actgataaaa tcgcttgaaa tatgcccggt tgtcaaacgt ggcgagtaca
    11821 attattttat ccatcctata acggacgggg taccttctct cgatcctgcc gtgctccgcg
    11881 aagtggcaac tggcatgatc aagatcatgg atctggaccg ggtcgaccgg atcgttgctg
    11941 ccgaggcgat ggggattccc atcgggtcca tcatctccca catgaccgat ctccctctga
    12001 acattgtcag gaaacgggaa taccagttgc ggggggagat cccggtgcac caggttaccg
    12061 gatactcgaa aggacagctc tttttgaacg gtgtgcggga gggagaacgg gtcgttatcg
    12121 ttgacgatgt ggtcagcacc gggggaacga caaggggaat ccttgccgcc ctccacgtgg
    12181 caggagcaga ggttgccgat atctgctttg ccatcaggaa agggtatcct gatatcggaa
    12241 ggccttacaa ggtgctggta accgtcgagg tatctgacag ggtgcgtgtg gttgaacaac
    12301 atctctgacc tggtgacctg gctccaagag aagagggcac ggtcagtcgc cctccagttc
    12361 ccggccggcc tcaagcgaac ggcagctggt attgcagatg cgctttcacg ccagggattc
    12421 tctgtcatca tcagcgggga tccctgttac ggtgcctgtg acctgtcgct tgagctgctc
    12481 gactatgccg atgggctcgt gaacttcggc cacacccctc tgctcattca tccggacgtc
    12541 ctgcacatac cctaccgggt cgacttcgat ccggttgtca tcagatctgc cctgcccttt
    12601 ctcaagggga aaaggatcgg attaattacc actgcccagc atacccacct gctcccggag
    12661 attgctgagg tgctcagaaa cgaagggtac gatcccatct ttcagtgtgc ggggggaaga
    12721 acgccagaac ccggccaggt gcttggttgc tcttttgcgg ctgcgagaat tccgggggta
    12781 tcggagatcc ttttcatcgg aacagggatg ttccatcccc tcggagtcca gcttgccacg
    12841 cgggctcatg tggtggcact tgaccccttc tctgggcaag ccgttgaggt cgatgcaggg
    12901 aagtttctcc ggaaacgcca tgcgctcatc gagaaagcac gggaggctgc ccatatcggg
    12961 attattatca gcacaaaaca gggccagaac cgttgtgacc tcggaacacg cctcctctcg
    13021 ctctctgaca gggcatacct tgtcttcatg aaggaggtga gccctgacga gctcctgaat
    13081 cttggatttg aggcctacgt gaataccgca tgcccgagac ttgcatacga tgaccaggtc
    13141 aggttcccgg taccggtcct cacccccgct gagtacgaaa ttctctgcgg gacccgcacc
    13201 tgggaagact atgccatcga tgaaatgctc tgaccatgaa actgaaacac cttgagaagc
    13261 tccttgagaa cctcgaggga ttttccaatc cctctcctct ccttgaacag tacaggacac
    13321 cggctcctct cgctgcacgg ctcctgttcc atgcagcgat gaggggagat atcgagggaa
    13381 aacgggtctg tgatcttgga tgtggcaccg gggtccttgg cattggggca gcactcctcg
    13441 gttcccgtga cgtggtcgct gttgagatag atcctgatgt gctcggaatc gcccgtaaga
    13501 atgcccgcca ggctgctgtg gagatccatt tgatcgaagc agaggtaaaa tatccccagg
    13561 tcctccccct ggttggagca tgcgacacgg tcgtgatgaa ccctcccttc ggggcccagc
    13621 agattcatgc cgatcggccg ttcatcgatg ccgccctcgc aatagcaccg gtaacgtacg
    13681 gtatctttaa cgcgggcagc cgttcattca tcagctcgta tattgccgga cgggcagaga
    13741 ttgacgaggt gattggcggc ctgcttccga tcaggcggac gttcgccttc catagagaag
    13801 aggtcaggga gattccggtt gagatatacc ggatcgtatt gcaccatgag acataaccct
    13861 taataagagt ccatgatacc gccaggggta tacccttgtc aggttcactg acgcgatatc
    13921 tgttctgttt ttttggggat gatcaacctg gtctggtacc gtataatcaa ttgaggcctt
    13981 ttgaaaggag acatggtgaa cctcgataag agaagggggt tcatccccaa aaaatccgat
    14041 acgatcatga ggttccctaa aaaaacccgg aaaaaggaat gctgatacgc ttggggcaat
    14101 tgccgcacgt gattggaatt cacagagaaa ttatagggat tcaggtataa cagtcatgaa
    14161 atagagatat actgaggttt cggtaaggat atgatcacct ctgttgaacc accacaatcc
    14221 tgtcataccc tggtagttcc gggaacggat gatagacccg gcaggatatt gtctcatgcc
    14281 catgaaaaag gagagtgccc atgacgcgaa tgcttgtaac cgggggtgcc ggattcatcg
    14341 gcagcaattt tatccgtttc ctcttaaacg acaggagcga tacggaaatt gtcgtcaatc
    14401 ttgacaagct tacctatgca ggaaacctgg agaacctgaa agacattgcg ggaaacccgg
    14461 cataccgttt catcaggggt gatatctgtg atgcggaact ggtatcgcgg attctcgatg
    14521 aattcgatat cgatacggtg gtgcactttg ctgcagagag tcacgtcgat cgttctatca
    14581 gcgaagggct gtcttttgtc accaccaacg tgaccgggac ctgtagtctg cttgaagcag
    14641 cccgcaaggc cgggatatcc cggtttatcc atgtatcaac cgatgaagta tacggcagta
    14701 tcaggaaggg atcattctcc gagactgatc ccctgaaccc ctcaagcccc tattccgcca
    14761 gcaaagcggg ctcggatctc cttgccctct cctacttcac cacccatcat gtaccggtca
    14821 tcgttacccg gtgcacgaac aattttggtc cgttccagta tcctgaaaaa ttgatccccc
    14881 tctttaccac gaacctgatc gaaggaaaaa aagtcccggt gtacgggagc gggatgaatg
    14941 tccgagattg gatctatgtg cttgatcact gccgggcgat tgcgtgcatt ctgaaaaaag
    15001 gtatccctgg cgagatatac aatattggcg ggggaacaga actcacgaac ctcgagatca
    15061 cacgaaaaat actcgaacaa ctgggaaagg atgagacctc catacaattt gtcggagatc
    15121 ggaaaggaca cgatttccgg tattcccttt cctcggataa gctgcattcg ttgggatggc
    15181 agcccaggtt cacgtttgat gaagcgctgg aacatactat ccgttggtat aaggagcagg
    15241 aatggtggtg gcgaaaatta aagtgactat gcagcaaaac ggtcaccgga ccattattct
    15301 tggcgccaat ggcatgcttg gtcatgcctt ccaggtcgtt tgtccccagg cgttgttttg
    15361 tgggagggag gtggatatca ccgatgaggc gggcatcagg tcactcttca ggcgattaca
    15421 gccatccctt gtgatcaatg ctgcagcgta taccgatgtg gacgggtgcg aagatcaccc
    15481 tgaccatgcc tttttagtga acggtgaggg gccgggatat attgcaggtg catgccgcaa
    15541 aacaggagca tcgctcgttc atttcagtac cgattacctc tttgacggcg ataaggaggg
    15601 atacctcgaa tccgatcctc ctcaccccat caacacgtat ggacaatcaa aactcctcgg
    15661 tgaggaacgt atcagggaaa cctttgatga ctaccgcatt gtgaggacct cctggctctt
    15721 tggtcctgac gggagaaatt ttgttgatac catgctccgc ctctcaaaag agcaggagat
    15781 cgttcgggtg gttgatgacc aggtggggcg ccctacctat accagggatc tggcagcaaa
    15841 ggtgctcctt ctcgcagacg aagccccggg catctaccat gttaccaacg agggcacctg
    15901 ttcctggttc gagttcgccc ggcacatcat cccgaatgtc cttccctgca catcggatga
    15961 gatccaccgg aaagcaaaac ggccccgttg ttcggtactc ctgaacacca aaacagcgcc
    16021 tctccgtcac tggaaagaag cacttgctga ataccttgcc ttactaggga gagaaccacg
    16081 agagagatga tctcctcacg aggacgcctg gggtatatcc cggtattctg gtttctcctt
    16141 cccgggctga tatcgtggtc atcacctggt aatttttaga gagagcccag ggaagcaatt
    16201 gatgcatcga tcacgtttgc tattccacag gagacatgac agggaacgat cagatcaggt
    16261 tcttgagctt ctatcgaacc tctcggggta ttgggagagg attttacatc cctactttct
    16321 tttaaaatga aatacaaata tccatgaatc ccggaggctg gattggtgca cccggcagga
    16381 tgtctatgaa gatcatccag accccagtaa gaatcggaac aaccggcgga gtcgaggagt
    16441 atgtccacca cctctcgaaa ggccttacct cccgcgggca ccaggtcact gtcgtctgtg
    16501 ctgctgcagg agagagcgcg ttgaaagatg caggatatcc caggaaactg ctgagatcat
    16561 ggttcaggct ggcgaacacc gatatcaccc ccgccctgct cccgaccctg ctctccagtg
    16621 attttgacct catccatacg cacatcccca ctccctggac tgcagagata agtgcccttg
    16681 tctcaagaat acgaaataag ccactgattc tcacctacca caatgatatc cggggagagg
    16741 ggatctacgc tcttgttgcc tggacctata accggttcat cctgccgctt gtgctgagac
    16801 aggccagccg gatcattatt acccggactc gtcacctgtc tccttatctt gccccttttt
    16861 cagggaagct ggtatgtatc ccccccggtg ttgacagcca ttttttttcc ccaggtaaag
    16921 atcctggaaa aaggggagat cttttttttc tcggaatttt tgacgagtat caccagtata
    16981 aaggctttga tctgctgctg caagcgatcg ccttgctcgt gcagcaggga accgatctca
    17041 ccctcattgc aggaggcagc ggaccccttg tagggaggta cagggaactg gctgctgacc
    17101 tgtcaatcga gagaaacgtg atctttcccg ggtatatccc gggcggggaa ctcccgtcct
    17161 attatgccgg aactagagca tttgtccttc cgtccacaga tccctcaagg gaaggatttg
    17221 ggatcgttct tcttgaagca atggcctgcg gacggccggt tatagcaacg gatattgcgg
    17281 ggatggctgc cgagatagaa gaatgcgggg ctgggattat tgtgaaacga aatgatccat
    17341 ccgaactggc tgacgctatt cgccgcctca tcgataataa gcctctggca ggacgcatgg
    17401 gcgatgcagg gaggagactt attgaggaac ggtttggctg ggagataatt gcaggggagg
    17461 ttgaaacact gtatgccgga gtactctgag agcgagacag tggctttcgc ggggctggag
    17521 cagagagaga taccctattc tggcaatagt gagaagatga acgatcatcc cctggtgacc
    17581 gtagtggtgg tcaactacaa cggacgacac tttctcaggg agtgtcttgc aagcattacc
    17641 aaccagacct atgctccact cgaggttatt ttggtcgata atgcctcgtc tgacggaagt
    17701 gtagaatttg tgcgggcctt atttcccggc atccatatcg ttcagaataa cgaaaatcgc
    17761 ggtttttccg ggggggtcaa ttccgggata cgggaatcga atggaacgta tatcataacg
    17821 ctgaacaacg atacccgaat ggattcccat tgcatcaggt accttgtcca tgcgatgtcc
    17881 ggggatgaca ggattggcat gggcgtaatc aaaatgctct tcccggatgg gaccatcaac
    17941 tcgactggca tctgcatatc ccgaagcggg gctgcctggg atcgggatat gttcaagcgc
    18001 gatagtccgg aaaatagtcc agggggagag atcgtcgggc cctgtgccgg ggcagcgatg
    18061 taccgacgat cgatgctcga tgcgatcggg ctcttcgatg aagatttctt ccttttcatg
    18121 gaggacgttg atctcgccct ccgtgcacga cttgccggat gggagtgcgc atatgtgcca
    18181 gaggccgtgg tataccatca ccacgggggg acggcagggt tcatgtctga tctgacagtc
    18241 tactatggaa accggaacat tctctggtac ccggtcaagg atttcccagc agggtacctt
    18301 gtcacatcca ttccctggat aatggggagg accatcgcag taatccctta ttatgctgtg
    18361 agagggcagg ggagggtcat cctgaaatcg aagatcgatg gcatgaaagg cataccagac
    18421 atgattcgga agagacgaac cgtggaaagg atcattactc ccggtattgt aaaaaaaata
    18481 ataagaacat ggagttcagt acaaaggagg tcagatgtgg atcactgaat accgggatct
    18541 catttccata ctcgcaatca gtgatctccg tgtaaagtac cagagttcgg tactcggatt
    18601 cgcatggtcg cttgtgaatc ctctcctgat gatgctggtc ctctacatcg tcttttccaa
    18661 tgtgttccac atgtcggaga accaatatgc cctctatatc ctgatcggga tcgtgagctg
    18721 gcggttcctc cagaacggat catccgcggc tatgacttct atcgtcggaa aaccaggtct
    18781 cgtcaccaag gtatacatcc cgcggcaggt tcttgtcctg agctcagtcc tctcaggttt
    18841 tgtcagctcg ctgcttgaat tcctggtgct catctgtctt ctggtcgcct tcagcgtccc
    18901 gatctctttt gtcgtgctcc ttttcccggt tatccacctc ctgttcctct tcctgctgta
    18961 cggactctct ctcagcctgg cctcactcta tgtgtactac cgggacctga accagatctg
    19021 ggaggttctc ctccaggcag gattcttcct ctgccccatt gtctaccctc tcagcaccat
    19081 tccggaacaa tatctcccct tctatatgct caacccgata accgatttca tcgcgattta
    19141 tcgcgatatc ttcttgtacg gcaagctacc gtctcctgcg agtatcgcaa ttatcgtgat
    19201 agccggaatc atcctttacc tgatcggacg actgttgttc gggaaactcg aacgtcgttt
    19261 tgcggaggag gtctgatatg gaggagatca cacgagatac aatcgatgaa ggtgatgcaa
    19321 tcatcgtgga acacctctcc aagcgctaca ggatacccca tgaacgaaaa caaacgatct
    19381 ttgagcacct gttcagcatt ttcaaagggg gtgcctattc atacgaggag ttttgtgctc
    19441 tccacgatgt atcgttccgt gtccggcatg gagagaccta cgggatcatc ggaccgaacg
    19501 ggagcggaaa gagtaccctc ttaaagatcc ttgcaggcgt tctgtacgcc gacggcggag
    19561 tcaagatcgt ccaggggaga attgccccct ttatcgaact gggagtgggt ttccagccgg
    19621 acctcaccgc taaggagaat atttacctgt acggggcaat catggggctg aaaaagcgtg
    19681 agatccggga gcgttatgac gagatcattg aatttgccga gctgaaacgt ttcgaaaata
    19741 tgcgcctgaa gaatttttca tcaggtatgg tggtgaggct cgccttctca acggcaatcc
    19801 agaccgatcc cgacattctc ctcgtggatg aggtactttc cgttggagat gaatcgttcc
    19861 agcagaaatg caaccagaag atcggagagc tgaggagcaa ggggaagacg atcatcttcg
    19921 tctcccattc gctcgaaacg gttcacaccc tctgctcccg ctgcctgctc ctcgacaaag
    19981 ggcaggtgat ccagatcggc cccacacccg atgtggtgca cacctacttg agattgatcg
    20041 gatcgaaggg ggcattatca taggtccggt atccggattg acaggtgctc ttgaggaaat
    20101 acctctctct gacagcggcc ttcggaggac gtatccaaaa cattcccgta tcctgctttc
    20161 ctgctccgga tcaggtaaag cacatcacga atggatgcag agaggacctg cctatggacc
    20221 ctgatattct ggttattttt gaacagaaac tgcatctgtt cattatcgat cccctcctcg
    20281 gttttgatac ccctcaacgg aagatgtacg accttggcga aaagagcgtc catatcctcc
    20341 tcgaagaggg gttgtggggt cttgtagaca gtttccggga gaggcggagg gtccagcggc
    20401 ttgccaggcg cgtcacccgg gagtatgctg aaatcccttc cccgcgtccc tggagcggtg
    20461 aggagcttgt ttttccccag aggcctgata atccggaggt atcaatcgtt atcccggtct
    20521 acaacaacct gaccttcacc ttcaactgcc tcagatcgat tgttgagcac accactggct
    20581 cttacgagat tattgttgtc gacgatgcat catcagatga tactccacga ttttgcagtc
    20641 tgcaaaacga actgattatc gtccggaatg agacgaacaa ggggttcgtt gggtcctgta
    20701 acaggggcgc tgagatggca agcggtcagt atatcctgtt tctcaataat gatacccagg
    20761 tgacaggggg ctggcttgaa ccactgatac ggacaatgca gagagatcag gtcggtgccg
    20821 tgggatcaaa gctggtgtat ccggatggaa ggcttcagga ggcgggaggg attgtattca
    20881 atgacgcctc gggatggaat tatgggaggg gtgatgaccc agatcgacct gagtataatt
    20941 ttttaagaga ggttgattac tgctccggtg cggcactgat ggtgcgaacc gatcttttcc
    21001 ggcatctcgg ggggttcgat gaatggttca ctcccggata ctacgaagat actgatctct
    21061 gtttccgtat ccgtcaggag ggataccggg tcctctatca accaaaatcg gcagtgatcc
    21121 attacgaggg gatgaccgca gggagagagg gagccggggg aatgaaagag ttccagcagg
    21181 tgaatcgcga taaatttttt tcccggtggt ctgctgtgct tgcccggagc cacaaacccc
    21241 cggattcccg caacctgttc cataccagga tcaggagagc cggtcagaac gtcctgatca
    21301 tcgatcatta cctgccaacc ttcgaccgcg attctggttc actccggatg tattatctcc
    21361 tcgagatact ctcccgtctc gggtatcatg tcaccttcgt cggggacaat ccctcaccgc
    21421 tcgaaccgta ctgctccctt gtgcaggacc ttggggttga agtgttgtat actccctatg
    21481 tgtattcgat cgagaagtac ctgaaagagc atggaaagaa tttcgacgtg gtcctcctga
    21541 gcagggccca tattgcctgg aagtacatct atcatatccg gcgtttctgc cagagtgcaa
    21601 ggatcctctt tgacacggtg gatctccagt ttgttcgcga agcgaggcgg tacgagattg
    21661 agggggggaa aaaactcctc gcctctgtcc gcaagatgaa agagatggag cttcgcatcg
    21721 cccggctgag cgacacaact cttgtggtta gtccggtaga aaaggaaatt ctcctcaaag
    21781 agaacccttc cctccatgtg gaggtcctct ccaatatcca ccatatcttc ccggtatcaa
    21841 agggattttt tgaacggaga gaccttcttt tcatcggggg ttttattcat ccgcccaatg
    21901 tcgatgccgt cgtctggttc actgatgaga tcttcccgct ggttcaacgg gagattcctg
    21961 aagtggggat gtacgtcgtg ggggatgatc caccgcctca ctttcgtgca tttgacagcg
    22021 atgccgtgaa agtgacgggg tatgtacaag atgttaccag ttttttttcc gattgcagag
    22081 tatttgttgc cccactccgg tacggggctg ggatcaaagg gaagattaac cagagcatga
    22141 gttatggact tcctgttgtc accaccacga tcggagcaga gggaatggga cttattcacg
    22201 gggaaaatgc cctcatcgcg gatgatcctg gggattttgc cgatgaagtg atcaggcttt
    22261 accgcgatga agcactctgg aaccggatat cgtctggttc acaggagaac gtgaaaaaat
    22321 ttttctctta tgacactgca agtgaagctc ttttaaaaat cctgaaatct tcatccgagt
    22381 aatctcatgg gtaacaatgg gaggaatttg acgtatgatc ctgcactaat cgataaaata
    22441 ggcaatttga ggaactaaag gggaaattct tttaacattt ttaggaaaac attttttaat
    22501 aatgtagggt tctattctgg gattatattt caagagaaca agtcaagaaa aaaaattcat
    22561 tgtaatattt atctgatatt gaaagaaatc gtcttcaata tgaattggta tgttttcggt
    22621 ctcaccgcat tgtctgcttt tattggaagt ctgggtcaga tcgagttcaa acgtggatct
    22681 gaggtattag aagttggtat atcttccgtt ttattcaatt atcatattct tatcggccta
    22741 ttcctctacg caatttcaac tatcatatat ttatacgctc tgcggacggg acaactctct
    22801 ctcatttatc ccattattgc gacttcgtat atctgggtat taatattcgc acgattttta
    22861 ttcaatgaac ctgtaaattt cgtaaacctt ggaggggtgc tatgtatcct cgctggaatt
    22921 acccttatat cctatcggta ggtaatacaa aataggatta ttagtaaaaa aaagatcgtg
    22981 ggatttttgt gaattttcat accacactaa aaaaaatttt actccaacat ggttaccttc
    23041 tccttctgat tgttcttgtt ctaggatcat ttgttttgag gattgcgctg cattctcaat
    23101 atcttgatga ttgggattca atacaatttg tgcttgcttt gaaagaatat tcgattccag
    23161 cgcaccaacc tcatcctcct ggttatccga tttatgttct cctgggaaaa attgcgaatg
    23221 cagtaattcg tagtgggcag cattcgttca ttgttatttc tgcaaccttt ggctcattat
    23281 cgttaattcc aacatattat ctagggaaat accttttcaa tgaaaaaatc ggaatactcg
    23341 cagcctttat cttatctctt acccctgctg ccctgctatt cagtgaagta gtgatgtcgg
    23401 atattgtcag catgtttttt attgtcttaa ctttatgcct actctacgtg ggtattgaaa
    23461 atgatatctg tttgtacatc ggttcattta ttctgggtat cactataggg gtaagacaaa
    23521 cagatttctt attgctaccc ttattcttct atattctata ctccaaaggg aatattaaaa
    23581 aaagtatact ctcatccttt attgcaatat ttgctatttt aatctggcta gttcccgtat
    23641 taattattac aggtcccgaa gcatttttaa atgcgcaacg aagtcaggga aatgtagtct
    23701 ttgggtatgg aatatggaat actcttagtt ggccaatctt gttgaacttt cttcattcaa
    23761 tcttgagatt attgatatta ttgataaaag gctggtcgtt atcacttttt ttatttgtag
    23821 cagtcacaat catcattgtt gtatacccat acttgaaaaa ctatagattc gatttagata
    23881 aacgaaaatt atttttcgtt ctatggattt tgccatatct tgcatacttt atcctatttg
    23941 gacaattatt actatacatt cctcgctatc ttttacctat tttcccggca tttgcactaa
    24001 ttttcagtta ttctcttgtc aaagcgataa atataagcaa taaaaagatt atgaagactt
    24061 ttttaatagt aatttttgct atatcgatag gatttatggg atatcaggca gtatctactg
    24121 tttacaggct tcatactacg atgcctgcac ctgttcaagc agctaatttt ataaaggaac
    24181 attataatcc agaaaacacg gtaattatcg gagattatga atcttttcgt cactattatt
    24241 attatttatc agaatatgat gtacgatata ttggtgtgat gaatacaaca gaagcgtatt
    24301 atttgttatt agaaaataag acggttgttt ctgggattga tttgggacct tctcccaagg
    24361 agattcattt attttcccgg gatccaaaaa tttatccaaa acaccatgac gtcagattgt
    24421 tcatatttca actcgctgaa aacagagaga atttctattt tggtaatggg tggtatactc
    24481 gcgaaatgcg agatgctatt cccttcaact ggataggtac aaatgcctcg ctttacattt
    24541 actccgaaga aaatcgggag gtggatctga actttcaggc aatcagtgcc attcgcccaa
    24601 aggttcttga tgtttacgaa gggactcacc caatcgccac tttcacgata tctcatgagc
    24661 agctaacgga tgtcagactc ccccttacac tatccaaagg aacaactatt gtattttttt
    24721 ctctacctga gggttgcgag aggccaagtg atttccaaga ggttaagaat ccagattcac
    24781 ggtgcttggg tatagcaata caaaatatca cgttcattta agcgaatggt ccattcggcc
    24841 gtaaatggaa aaaaatttgt atagtttctg gtgcttcacc ccacatgaat tcccgaacct
    24901 gaatttcaaa agagagtagc attccagaat cctcatccag tttcttcact aaaaaaaaga
    24961 ttcaggagat atcgtttctt accttggcat tcatggggat tcgataagta ttttgttatt
    25021 cgtaccaatt attacgtgag gtataatgcc tgatccgacc aataaacgtg aattgatact
    25081 cttttcactg aaatcatgcg aggccggaga aggcacagcg tatgtcaagg agtatttttt
    25141 tacttcgctg atcgctgacg ggagatcaat cctcgacctt gggggtggca acagaggtgg
    25201 gacagtcgct cttggagaag ttgcacagac tgtcaccggg atcaccaggg aaaaacactc
    25261 ttttctggtt gatcgggtcg aaccatcacc gaatgttacc ttcctggcag gagattcgag
    25321 ggcacttccc ttccctgata agacgtggga tattgttctg ttgttctctg gggaatccat
    25381 caaagggtca tttgacgcgg ttcttgccga ggtctccagg gtcctgaagg atgatggtat
    25441 ctttattacc acggtccagg ctatggacag cgcctatgga aaaccgggag gggaagggaa
    25501 ataccctctt tccgaggact ccccttcctg gagagccagc atcgaaaggg tgtttccttt
    25561 tatcgtcggt ttcagccaga ccggcgatgg aggctgtggg atggtccccc tttttccggc
    25621 acaaaggggg gaaattttag agaatatccc agacaagcag ggaccagacg ctccctgtct
    25681 catcgtcgcc gcaaggagac ctgtgacact cccctggctg gaggggatga cctgggcagc
    25741 agcagggaga tctgatcaac ctgtctttga aagggagcac gagaagcgga tcgcatttct
    25801 ggagtcggta atagaacaac gggaccttgc gttgcaggtg gcacgggaac ggaacgagaa
    25861 gctcaaggag gaagtggtcg agatgaagcg gagcgttctc tggcggtgtg cgatgacata
    25921 ccataccgca attgtcgaac cgctccttgg tcatgccacc agaagaagga ggtggtatga
    25981 tgcggggttg tccgggctcc gcctggtgtc aaatgaaggg gtgtccgggc tgctgtggca
    26041 cgtgaggggc ggagcggcga gaaaccggca tagtagtttc tcccgccttt tccaccgttc
    26101 ggcagaccgc tcaacccgtt ccatccggga acggatctcg ctgattatcc cgacgaaaaa
    26161 tgctggacac gagttccggt atctcctgcg aaagatcagg acacagaagg gggtcagcga
    26221 tgttgagatc atcgttattg acagtgggtc ttccgatgaa accgttgcta ttgcccgatc
    26281 gtttgattgt catgtcattg aaatatcccc cgaacagttc caccatgggg ggaccaggaa
    26341 tgctgcagca tcccacgcaa caggggagta tatctccttt acggtgcagg atgcacttcc
    26401 ctgcggagag ttctggctcc gtgacctggt cctgcccctt gcagaagaca cctccgtgag
    26461 tgcagcaacg gcccggcagg tgccccgctc cgatgcggat ctctttgcct gtttcaatat
    26521 ctgggcaaat taccaggcga tagatttcct gagagataag gtcgtttccg cacctcctga
    26581 ttattcctcg ctctcgctga tgcagtggag gaaacttgcc cagatcgatg atgtctgttc
    26641 tgccttccgg gccaggatct tttcagagtt ccgttttctt accgattatg cagaggatct
    26701 cgatatcggg gtccgcctgc tgaaagcggg acacaaggtg gcgtttgtcc attcttctgc
    26761 agtgatacat tcccataacc gcaccgctgc ctattttttc caaagatcat atgctgattc
    26821 attcaccctc tccacggtat ttccccatga cgggagtttt atcccggaga atcgctcccc
    26881 cgaggagata ctcgggggga ttttttcact ctaccagacc ttcaatgcag cggtgaaaat
    26941 tctgaaggag caatccatcg ctgaactgag tgaggagatg acacagcaga tcaaatattt
    27001 gatgaagagc gagctgtcaa ggcccgacac agctgacggt gagatctctc ctggtgaacc
    27061 atcgatggat tcgttgtttt acctgctaca ggagagactg cctttccgtg ggcctttcca
    27121 tcaggacaca cgactcctca ggctcttctt caataatctt gacaccttcc gcgactttcg
    27181 gaaacgatgc gggaatcccc cgctggatcc ggtcgagatc gaagcggcat tatataaatt
    27241 gttcgcagcg gcttcgggga tctcgcttgg gggatacctt gcactgctca aggagggaaa
    27301 ttccgtccat actaccagcc tcgaaaccct ccacgccagc ctgacagagg ggatctgatg
    27361 aaagtcctct ttgcggtcca tcagttcttt cctcaccatt ataccggtac tgaaagggtg
    27421 gtgctgaacc tgtgccggca gctccagcga atggggcatg atgtgatcgt cctcacctac
    27481 gccatcgatt cagctgaggg gctgtacccg aggggtgcat ttcttgtggg gaagtactca
    27541 ttcaaaggga ttccggtcat tgccgtccgt caccgggaac ctcccgaaga cctggatttt
    27601 atcttcaggg acgaggggat gcaggccctg ctggaatcaa tcatcgagga tgagcaggta
    27661 gatatcatcc atgtctgcca tcccatgcgg gtcggatcga tcatccccgc tgcgatatcc
    27721 cgcagccttc cggtcgtcgt gacactgacc gatttctggc ttttgtgccc aaaattcatc
    27781 gccgtgacag ggaagggaat cccctgtgac gggccatggg gagggaagcg ctgtgccgag
    27841 gagtgttatg aaccgtcatg gaaataccgg atacgggaaa gattgcagga gatcgatggg
    27901 gtgaaaaacc aggtatcggc gttcgtctca ccgactgatt ttctcaaaag gatctttgag
    27961 ggcaaccggt accgatcggt gtatcgtata ccattcggcc ttgattacca ggacatccgt
    28021 atccaggaga agaagtataa cgatgattcg gcagttgttt ttggctacct gagcactccg
    28081 gtccgtcaca agggagccca tgtcctcatc gatgcattcc tccgtgcgga tatgccgaat
    28141 accagcctga aagtgtttgg agatgacagg gctgaccggg aatattatga gaacctgaag
    28201 tccgcatccg gtggtgatcc aagagttgag ttctgtggcc ggtaccatca tgaggagctt
    28261 tcatccatcc tcggttctct tgacgtcgtt gtcattccct cgctttggtg ggagaactcc
    28321 ccccttgtcc tcctgactgc cctcgcctgc ggggtgcctg caatcgtgtc agaccttgga
    28381 gggctgaccg aatctgtcca ggatggcgtg aacggatact ccttcaaggc tggtgacatc
    28441 gatgcacttg caacggtgat ccggacgata gcatcctcac catcagggtt gcaggagttg
    28501 cgggcgggta ttacgtatcc tgcaagaatt gaagaagagg cagtacagta tgagaagatc
    28561 tatcagaaca tatggaaccc tggcgagaag cacgggagcg ctgggaaaaa gagacaaagg
    28621 agtcgggtcc cgtgaatcgt tcctgctcct ccggcacgaa aaattctgaa ggtggtcctc
    28681 tctcatgaaa cgttgtcttg tgctaggcgg gaacggattc ctcgggtcac atcttgctga
    28741 agcactggtg gtttccgggt tcgaggtgac tatctttgac agccgggagc aaaagaggcc
    28801 ggatatcagg gaggattcga ttggatacag acaaggggat ttcctgaacg aaaatgacct
    28861 ggcccgggcc cttgccggga ctgattacgt cttccatttc atctcaacga cgttaccggg
    28921 accgtcactt gagtcaccgg tatatgatat cgagtcgaat gtcatcggca cggtccgtct
    28981 ccttcagcag tgctgcaagg caggggtgga aaagatcatc ttcccttcat cgggggggac
    29041 ggtatacggg gagccagaca ccattccggt ccgggagacg gatccggtgc ggcctgtcca
    29101 tccctatgca atctcgaaga tgacgatcga gatgtatctc cgttcgttct atgaactcca
    29161 tggccttgac tacctgatac tcaggtatgc aaatccctat ggagagcggc agatgccatt
    29221 cgggaaccag ggggtgatcc cgatcttcct ccatgcaata gtgaagggca gatcgccggt
    29281 aatctacggg gatggatcga tggtaaggga ctatatttac atcagtgacc ttgtcgatgc
    29341 aacgatcgcc atcgtaaaga aggatccacc cgagcggctg ttcaatgtcg ggtccggcag
    29401 agggacgtcg gtgcatgaac tgctcggtac catggcggaa gtcctcgggg tggagatcaa
    29461 tcccctctat gccgatccac cgggcacata cctatcccgg atcatccttg acatctcccg
    29521 catgcagcac cttacgggct ggaagccccg ggtcagtctt gccgaaggca tcgagcggac
    29581 ctaccgctgg ctgaagagcc tgccctgaca atctgaaaaa aataacatga ttcttttgag
    29641 agcaccaagc ataaaagatg aaagtgccgg aggcattttc atataaacct cctatgagcc
    29701 ccgatttcgg gatacgggca taaattatga aaattacgaa gttattttca ttggaaaaat
    29761 ggaattataa aatcctgaag gcactggatt ttattcccga ttttccatca ggattgatga
    29821 cgacaacatc atacacgccc ttcgtggcgg tggaaggtat cgggattttt ccggtgattt
    29881 tgagggaact tacgacaacc tcattggttg cctcgatggt cggtgccccg gactttctga
    29941 gctggacctg agccccgggc ataaagttcg taccggcgag attggtcacg gtcaccgtgg
    30001 ttcccaggcg tcctgatgat ggctggatgc gcgagacggt tggagcaggg gtactgccag
    30061 cagtgacggt aaatccacct gcaagggtcc cggattgtgt gtcgggattg atgacaacga
    30121 catcccatga tccgattgtg gcatccgagg gaagtacgat cgtgcaggtg atctgggtgt
    30181 ctgacaccac gccttcactg gttgctgcga tgacctgccc gtctttcctc agctcaacgg
    30241 cagccccgct ctggaacgag gatccggcaa ggttggtgat gctcacggtt gtaccggcag
    30301 ctccggtatt cggggtaatg gcggtcaccg ttggtgcagg cagtcctccg gaggttacag
    30361 agaagccgtt tgaaaggatc ccatactggg tgtcggggtt ggtaacgaca acatcccatg
    30421 atccgactgc ggcatcagag ggaatgtcga ttgtgcacgt aatctgcgat gcagtcacga
    30481 caagttcacc cgatgcctcg atgacctgcc catcgttctg cagctccacg gatgctcctc
    30541 cctggaagtt tgtgcctgca aggttcgaga tgctcacaga agacccgacg acaccgctgc
    30601 ccggggttac cccagtaacg gtcggtgccg gagcgccctg cggcagggta agctcgatct
    30661 ggtgagcggt atcgccatca taaatctggg ggttctggct gttggacgaa taggctgcca
    30721 ccttgggttt tgcatagaag aatccttctt ctgccgagat gtacgtgttg gagttggtgt
    30781 cagccgacaa gctccggaga ccttcgagca ggaagtaggt gaagtatcca ttctggaggg
    30841 cagatgattc gccagatagt tcggcatcgt tgcaggccat cagaacggta taccggctgt
    30901 cactgaggtc ccgcgagaag tcctgccgca gcccgtcacg ggttgacggg ggggttgtcg
    30961 tggtatcctg caaggagatg aaccgggaac cggtgtcctt cgtcatcccc cctgagaagc
    31021 aggtatcgac gatgaggagg gtatttttcg atggcacggc atcgagccat gccttgagct
    31081 cgtcatcacg gattgccccg ctcagaaacc gcgtgtccca ggcaatgatg tactcatcgt
    31141 acccatcact ctcatcatat ggtgacagat ctgacgcata cgtgccatgc gatgagaagg
    31201 aaacgaccac gatatcatca gtccccgact tggactgcag ttcggctatc tttgactgga
    31261 ttgcattctt tgatgcctgg ctgtcaatca gggtcacgat atgatcggcc gggatggcac
    31321 agtcgttctg gaggtacgtg gtaaggtcac gtgcatcatc atcgcagtag ctgagatcat
    31381 tgacaaacct gaagtccgag atcccaacga tgacggccca gacctcagaa tcagctctga
    31441 ctgagggaat ggccctttcg tcacttgcgg tggccgctgg caccgcaatg aaaagacaga
    31501 tgaagaggaa caggatcaat gttccaacct gacttggttt catacatacc tgagtgaaga
    31561 tgcaggatga tattaattcc tcttcgttag tgttcatcag atttttttga ggatatagag
    31621 gtcctggtgt ccccaccagt acgctttttt gtagcagacc aggggggcct gggtcatgcg
    31681 gtacacctcc gggatgaccc agtccggcag ggtaactgtg gttccgtact cctcgcttgc
    31741 aaggtctttc tgctcactcg tgggtttaaa gagaaatccc tcggggggca taacggcttc
    31801 cggctggtat ttgaggctct cctcgccgtg ggttgtgaag atgagatatc cggaaggggc
    31861 aacgctgcga tagagagtct ggatccatcg tccccagctc gaacgcggca tatgcgagaa
    31921 gaatgagaga gcaaagatca catcatattc cctgggaaac cggacttctt ccgggaccga
    31981 atgggagagg acggtatcga ttcccatcgt ctgctggata aatccggtgg cctgttcgtg
    32041 aatgtcgcag gcggtgacat gggcaacggg gaccattctc ggcaggtggc gtgtgacaca
    32101 accgtacccg gatgcaaact cgaggatctg tatcgggcca tgctccaggt gcagtacgtt
    32161 ggtaaggatc gaagacagcc tgcgtgccga attattgcca tcgttgaaat aataatggac
    32221 tgcatccttg ggattggtga aatacggatt tgtgaggacg aactggaaaa taaagtcgtc
    32281 agggtggata tcgggtacga cctgaaagga ggctgcttca tcccggcaga atcccagtaa
    32341 ctcttcggtg aggagatcct tcccttccgg taagggtatg gaattggtat catgctcatt
    32401 ctgttcttcc ttcggtaatt cctgaaccgg aggatttttt ccaaatatcc gccggaaata
    32461 cgctctcatg tattatacat cggctcaatc ctgacaaata ctcttgcacg atgtggaagg
    32521 aaaaaagatc aggggaaagg attgaggtag ttccccgaat actggcttat caggtcggca
    32581 aaggacgaag cgaggatctc ctgaccgagc tgcccgtcaa caccacccca ccagtccatg
    32641 tagttgccga gctcctgccc ggcatactgc tcggcgttga tggttgcggt aatactctgt
    32701 gctgcaatca acgattcatc aggatcccgt gaagtgatga tgtaatttga gaagagctgg
    32761 gttgcggtcg ggcgtcccca tgctgtaccg gagataccct gggcaacgaa gctggtcgaa
    32821 tcacccaggt cgtcggttcc aaatgcagaa ttgtaggcat actgggtgac atcattctcg
    32881 gttgagatcc ctgacagggc aatccactgc gagatggtgg catcattctg gaacctggca
    32941 acattttctg actcctgcca gacatccggg ctggatccgg caagccagag tgcctggcca
    33001 aggtccatgc cgtcgtcccc gtttgccaca ttcatcccca cctggtatac cgggccgttt
    33061 cctgatactg cactcgcgat gatcgactgc ccgatgacag aatcgtctcc tgtgctgtcg
    33121 acatagttct ggaggtactg ctcgaccaga tctaaaccag acgctgctgc ttcaaaatac
    33181 tgcgagacga tcccttcatc acccatgtaa aagaggacgt ttccctgtga atccgggttc
    33241 tggaagacct ggctgccgat accgctcatg ctgatgtact gctcgatgaa tgcctgatta
    33301 ccttcaaaga ttgcataatt ctcggcattc tgggtgacgg tcccttcggc gctgtcggca
    33361 ctcatggaga tcgactggct gagatcgaca ttgtcaccgg cgatggcggc ggtgttcgtg
    33421 gcatcctgtg agatgtctcc cccggcaatc gcggtcatct cgatctccat gataacctcc
    33481 tggtcatccc cggttaccgc gacgctgttg agtgccgatt gatccaccgt tccaggggcg
    33541 gctccctgca ttgagatcat gctgaccgtt gcaaccaaac ccccggtagt agcgacggca
    33601 ttataggcag tctgggtcag atctccgccg tcagcggtga gcactacttc caaatcagcg
    33661 gggtcctctc cggagacgac cggaactatc agtagtcctg taatgataac aaagacaaaa
    33721 atatccttga ttctgttcac cattgtaata gaataatccc tcctttctcg gttgagtcaa
    33781 gataccatgg ctttacggat aaatttgtct attccgttcg ttagatacat tgcgaaggaa
    33841 aagggatcgg gatacaacag ggggaccacg gattcccttg aatgtgaata attgtcaaaa
    33901 aaagaaatag atggattgat tatccgatcc agttttcgtg atactggtat acgtaatcag
    33961 cagatgcatc gccaaggata tccatagcga cagatccggt gttgggatcc caccaataca
    34021 cccagttgta catcccctgg tatgcctcat cagtggctgc aagggtcgca acattgctct
    34081 ggactacatt gtacgttcct ggagcccagt tccaatcgag tgcagcggtg ttgtagaact
    34141 cctgatagga gatgtctcct cctgttgctg ctgcggaaat cgattggccg atagcgctgg
    34201 ttgcaccgcc atcatcccag ggattagtgg catagttgta gccgtactgg tcgacacctt
    34261 catcatcaga aatcgcgctg aactcaagtg cctgtgctat ggttgccgaa taaccaaagt
    34321 aggcgtcgtt gtatgcctcc tgatcgacat atgaaccatc tccaccgatc gacaccaact
    34381 ggccaaggac tgcattgtca tccaggtcag cccagttcca tccatactga taaacctcat
    34441 cagatgcaac gccgcccagg gagattgact gacttgcaat ctcattaccc caggtaatac
    34501 caacatcagt cccatagtcg atctcgttat at
//
LOCUS       DAOOCO010000004        27313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_1416_length_27313_cov_6.581297, whole genome shotgun sequence.
ACCESSION   DAOOCO010000004 DAOOCO010000000
VERSION     DAOOCO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 27313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27313
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_27313_cov_6.581297"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK154_00500"
     CDS             <1..443
                     /locus_tag="PK154_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09572.1"
                     /translation="LADGKGELYGDKAIYLIEPSDQFTLYRLIPPLVERIILTCRIYD
                     KSHLICHQSLAPVEFGKKAEGVGRAVIALKKDGVVFPGLSVRIRKDGQIVANDISNAN
                     GVVSFRLLYGNYEVLIVRSENNIDIFDFSFIPDLQGRQLDLELP"
     gene            621..2216
                     /locus_tag="PK154_00505"
     CDS             621..2216
                     /locus_tag="PK154_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPW09573.1"
                     /translation="MSVSSAIVAHHGIVAGIFDELEIGRIIDETIPKQGQHKLPHSLV
                     LKAMVLISLGFNERRLYIFPKFFNNIATERLLGPGILPEDLNDDVLGRTLDRIYEADS
                     TELFLKIALQVMQHLPFGTQLLHADTTSVSVHGNYEHIDGTPAIQITYGHTKDNRPDL
                     KQFVLSTITNQHGIPLFAKTYSGNASDKQNLIDSIQQLRAVVQTPDDAYFIADSALYT
                     ADNIRNLGQNIHWITHVPSTITEVKTLLHADVLMHPGKDTRYSFYETTSEYGGIRQKW
                     VLVHSKEMQARKEQTFETSLEKLDVKTAKSFKKLLGLEFACENDARAAAEHWAKNHPW
                     YQFTGFTIVPTSRKIEVKRGRPRNDEERCTVYTVQAEIVRNTEVIHAEKEKLGRFILA
                     SNELSLDPETMLDYYKNQSAVEKGFRFLKDKSFRISEVYLKKPERIEALSMVMVLTLL
                     IYSVAEWLIRTRLKERNEVVPNQVNKPTQNPTLKWIFMEFMGVIEVRMNVPGQPERQL
                     TNLNENARKIIAILGGRCENYYI"
     gene            2433..3557
                     /locus_tag="PK154_00510"
     CDS             2433..3557
                     /locus_tag="PK154_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HPW09574.1"
                     /translation="MDRKKISKEDFESIIANKKNVQEYLETDPELGETFCFGVRTASN
                     PDYLTKAMYEMYETSLSRKLAIQAVRSLYRSVGLGSVGLNNYLKNLGIHLSPAEFLLL
                     VFKLQHQQGWGAPFELIEQKEKRIILQTSKTFESQVMKEWKMPVCGIHRGWIEGVLTA
                     VTGKNWFCLETKCYAQGDDCCQFVADQTESSWKWKAQAIVKGESAITEYIDHKPLEGK
                     IKLIDDPVVMMPRFIFSSMTSSLSKIMGEVPAGGVNYRAYMDMGKENVEHYKKMGVTN
                     PNTLSDMAFAFYSQMGWFRIVKMDWNESEKTKTITLEHTVESESMGNTGKSVCYCTAG
                     LLAGIIEGAFGIKVRGREIKCRSKGDEHCVFEIRNRSGES"
     gene            complement(3633..4418)
                     /gene="minD"
                     /locus_tag="PK154_00515"
     CDS             complement(3633..4418)
                     /gene="minD"
                     /locus_tag="PK154_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HPW09575.1"
                     /translation="MVRVFTLASGKGGTGKTTVTINLGTSLAQFGKKTCIMDADIGMA
                     NLGLSLGLENVPVTLHEVLSGTASIKDAIYEGPGGVQVIPCGISLQGFQQSDPELLKE
                     VLKELTDSLDYLLIDAPAGISRDGVVPLAVADEVILVVNPELSAIVDAMKTKILAEVV
                     GGQVRGAIINRATPESIATVTQKMEKVLGTRVIGIIPEDQNIRKAASAKVPIVLKYPA
                     SDSSVAIKKLAAGLAGLTYEQKEMDSKDSFVDRFTKVIFRGRK"
     gene            complement(4488..4844)
                     /locus_tag="PK154_00520"
     CDS             complement(4488..4844)
                     /locus_tag="PK154_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09576.1"
                     /translation="MREQGNKYTENALQENNGTFVSGMQSVIGRYTIDSMVVAMTDGL
                     VVASVGNKNPEYDAAYYSSVFEGKSAQPEQGILLVPLNHRGVPLVGIARTREVIPEET
                     MVIIAKEMQSVFEQNC"
     gene            complement(5042..5578)
                     /locus_tag="PK154_00525"
     CDS             complement(5042..5578)
                     /locus_tag="PK154_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09577.1"
                     /translation="MTQFVCDKGRIRLAEFGSLEGDEALEKIVAASHWNSNAILHDLT
                     YAQLRLALEFNPSFKTFVKNKTQKRHMQEVFNEGRIRPSDAIRIKTSGKSSHDARERA
                     APFPFSSGTGPGVQPAEISRKNDQSTAWSQGDLLSHDLDLIESMDLESMTTKFRTSCI
                     QIMERLDLDHLIEENSKK"
     gene            5915..6262
                     /locus_tag="PK154_00530"
     CDS             5915..6262
                     /locus_tag="PK154_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HPW09578.1"
                     /translation="MLKQILGEFLNLEGVSAAVVVGRDGFVIESAVSGKMDIDALGAM
                     TSTGIGTSEAMGKELGKGQLNQLLVELDKGPIIVSPLSKDELIAIVAENASNLGRIRY
                     ELKKNKERLVAAL"
     gene            6336..6701
                     /locus_tag="PK154_00535"
     CDS             6336..6701
                     /locus_tag="PK154_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HPW09579.1"
                     /translation="MDKWGTSDEKGSHLLDDSRLAAVQKQQGVLTVLAFHEGFPVKSA
                     GTGDYEQVAAVAEDFLRAGRKMAREMKLGDITQIILESDERKCIIAPYGDLFLCLLTR
                     SDVHLGLLRLIIRDLQDMD"
     gene            7003..8940
                     /locus_tag="PK154_00540"
     CDS             7003..8940
                     /locus_tag="PK154_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HPW09580.1"
                     /translation="MQSESPGVESVVGGRPVVKNSISSARSEPIRKSISSAEPPEGED
                     AVVSINEFDGISGLILPKSATFKVDELKIQSRPHIFDLKSSGGIPAKKEDYLKGEGIR
                     SISEAAASMETSLPKKSILARLNPLTTLRPVITEYDPNLHGPLVDLTYQPRPGIEEIE
                     LYPVNEPYAYIRITYDHTTHENTYGILEPILTKAEEELFSEIKERIFERLDINTQDMP
                     DEQARTTLRNASDEVIGDFGIPLTPVQREKILYSMYKEFLGNGMIDPIMHDRYIEDIS
                     CDGVNVYLFVYHTRYESMRTNLRYKTAEELDSFVTKLAQRSGKYISIAEPMLDSTMHD
                     GSRIQMTLGTEVTAHGSTYTIRKFKSEPITPTDLIEWSTFSPLSIAFLWLAVEAGKSC
                     IFAGGTASGKTTSLNAISLFIPPLAKIVTLEDTRELKLPHPNWIPSVTRTSFDTEGKG
                     EIDMYELLKAALRQRPEYLIVGEVRGREALTLFQAMSTGHVTYSTLHADSVASIVHRL
                     ENPPLDVPRNMLSALHLVSVQVQARIGGQRIRRNKQLVEILDIDPRTNELITNDVFRW
                     HPVTDEITYSGKSFLLEEIMESRGWTESRMREELKMRQEVLEWMRVKKIREYRDVSNI
                     LIQYYRDPTKIMEKVRAELYE"
     gene            8933..9856
                     /locus_tag="PK154_00545"
     CDS             8933..9856
                     /locus_tag="PK154_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPW09581.1"
                     /translation="MNSFQRLAFRILGKYAGRKRDKYSDLRNSLLTARMKIPFEVYLS
                     TAYLASSVIGIVSAILLGTVTWLFNVPGMITYQGEVPEVLLSLNQYSLIIGTLIATIV
                     SFAIVAGIIFLLFLLYPGFVSGNRKRNIESTLPYAINYVTAMSTAGIPPAEIFRQLGS
                     STIYGESATEARFIALEIDLFGRDLIDALRVVSSTTPSFRMKEFLQGSMGCISAGSNL
                     TEYFRNKAEQYALENRQTQKMFLETLGLIAESYVTAMVAGPLFLIILQSIMSILSSQS
                     QPFMLYIIIYLIIPFGSIAFVILISTMTPES"
     gene            9925..10983
                     /locus_tag="PK154_00550"
     CDS             9925..10983
                     /locus_tag="PK154_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPW09582.1"
                     /translation="MPVDPDSMGRMGKLSFLLSRGTPASVDLGEREKFEFEQESSAII
                     SRIEKYKATEFGIGRLLKHPFRVITEKPLKSLFVTVPVALAFFFIGTAIVIENEGINA
                     LFTSTAIDDLAVFALLMVSIPLAILDFRDSWRTRHIEEALPNFYRDLAGMNDSGMTLP
                     NAVHLVAQSDYSTLTPYVRKLDNEMSWNTTFIDAIQRFRARLSTPLTDRSIDLIAKAS
                     KAGGDISEVLRAAAKDSYEFINLQTERRNNMLIYVVIVFISFLVFVFVIYILVTTFLS
                     VMATAGSAASASGAGSQFGANVNLPLYTRIFTHAALIQAFFSGLVAGQMGEGRVIAGL
                     KYSIVMIIIAWIMFRFFV"
     gene            complement(11133..12221)
                     /locus_tag="PK154_00555"
     CDS             complement(11133..12221)
                     /locus_tag="PK154_00555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPW09583.1"
                     /translation="MSKEDLDILATGRLIGIARKWYDQMLEGQIPSIALPTRTKQNIE
                     YDDTSEVWKYGDRESIRSAGTAKSALHLLKMAYVIWFLRKQIGENRSSTLRELYYISE
                     GWKKAKFQAQDESNFLIEDLEILTDMQREAFHLRPEEDGATIFGPLKLREMTRRGERD
                     IHCQDDVGEAGYQIPTNVETVELLEHDAKFVIAIETGGMYARLIENGFDGDHQAILIH
                     LKGQPARSTRRFLNRINTQFNLPVVVFTDGDPWSYRIYASVAYGAIKSAHISELLATP
                     KARFIGVQPTDIRDYHLPSDHLNDKDIGALRSELTDPRFASDYWKEQIELQLKLNLKS
                     EQQAFAAKGLDFVTKEYLPARLSDMGII"
     gene            complement(12214..14016)
                     /locus_tag="PK154_00560"
     CDS             complement(12214..14016)
                     /locus_tag="PK154_00560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HPW09584.1"
                     /translation="MSLAEELAKQQRSISVAEFFERNKHLLGFDSPTRGIITTIKEAV
                     DNALDACEEAMELPDIFLSVSRQDRDIYRIVIEDNGPGIIPDQIPFIFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTAGVPTIIVSRTGPEYPAHECAIQINIDTNEPEVLT
                     RREIEWDRTHGTRIQIEFKSTLAAKKRLIEYLRYTSVVNPHARIRLEIDGEVFMFDRV
                     SDEVIACPRAIKPHPHGIEVGQIRRMLQASPGTLESFLMSSFSKIGKKTAEEICRIAQ
                     LSCTADASSLAAKEQKRLIEAMQMVKVPPPPISQCLSPIGEDLIIRGLEKEFQMDFVK
                     ARTRPGSVFSGHPFIIEAAIGYGGKLAADGNAQILRFANRVPLMYQQGACAITSAISA
                     MNWKNYAVAQAGLPTGPLLILVHVASTSVPFTSESKDAIASIPEIEREITLGLQDLAR
                     DLKVFLSRRDRNRLQEDRARAVCAILPELAEKVAEIVELPKPDISSIEGRIMHKLIVR
                     KTVSEGIVRIEIKNYTAGQASLSLYTITRDSENNADPPPDFLTKMDEDFTRVWQVVVD
                     PGKVWTTTYSGSAHGTVEIRGIDPRNVVMVDRDE"
     gene            complement(14003..15235)
                     /gene="hisS"
                     /locus_tag="PK154_00565"
     CDS             complement(14003..15235)
                     /gene="hisS"
                     /locus_tag="PK154_00565"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPW09585.1"
                     /translation="MLQKPRGTRDFLPDEMEQRREIEGRMREVARRWGYREICTPEFE
                     ELDLFTIRSGEGIIQEMYVFEDKGGRPMALRPELTAPVLRMYVNEGKTLPKPLRWYYF
                     ADCFRYERPQKGRYRQFWQFGVELIGVDSALAEAEVIMLAEEILSSTGISFSIHIGHL
                     ALVRSLLSEIPEQEQKRIRMFLDKKNFDGLSDYLTSKGWDDLCTSLIQLTSCRNLNDV
                     FAITGDLPEKARIGELFSILDTTGIPYTLDPGIARGLDYYTGMVFEIFAQNLGTENQV
                     LGGGTYRLAHLFGGEDTPSCGFAIGFDRVMVARGDQGPPHQIMAGIAYTPDGRDWALA
                     VAREFRAAGIRTEFDLSERSLTQQLSRLSKSADYALIIGKREKENSLVTLKNLRSGIQ
                     QELTLPEAISEVTGSEPR"
     gene            complement(15242..15565)
                     /locus_tag="PK154_00570"
     CDS             complement(15242..15565)
                     /locus_tag="PK154_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW09586.1"
                     /translation="MSYLVYIALFGAAVVFFLWLRDARIFYRTGLPGYRKAAYRGILF
                     GALAILGVAVTIYSLELLGLGLILAALYLQGREEREKIWGSEETLDRFLGKAPLRKGI
                     KNKEA"
     gene            15615..16391
                     /gene="larB"
                     /locus_tag="PK154_00575"
     CDS             15615..16391
                     /gene="larB"
                     /locus_tag="PK154_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPW09587.1"
                     /translation="MQANRKLREALLRFQEGAISLEETEREIDGLRIEEVSDIACLDI
                     GRNIRCGIPEVVLAEGKESAHLADIALAHARSSGRCVISRLNRAQEKILRERAEKSGF
                     TLTCHETARMAVLSTGDPITLTGGIIAVITAGTSDIPVAEEARVIATEMGCQVCTAYD
                     VGAAGIHRLFPAIRKCQGAQVFVVAAGREGTLPAVVAGMVDRPVIGVPVSTGYGYMGK
                     GKAALASMLQSCSVLTVVNIDAGFTAGAMAALIANMVVGQ"
     gene            16388..16930
                     /locus_tag="PK154_00580"
     CDS             16388..16930
                     /locus_tag="PK154_00580"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HPW09588.1"
                     /translation="MTRAFYIGRFQPYHNGHQYVLEKIAGTADAIVIGVGSAQLSHEK
                     ENPFTAGERILMITESLKDIRVPLYVIPIEDIKRNALWVAHVRSMVPPFDIIFSSNPL
                     VMRLFSEEGIPVRSPAMYARELHSGTEIRRRILKGEQWENLVPAPVSEIIREIGGVER
                     VRQIARNDCDVTESPGGHSL"
     gene            16964..18241
                     /locus_tag="PK154_00585"
     CDS             16964..18241
                     /locus_tag="PK154_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09589.1"
                     /translation="MFGTPKEPEDVECALGELPSLLDSREKEISRTLIEKTQKQRNTI
                     RDLRSSLKELVKDLTSKEREEAYHPKLETIAKNTLPLFERAMFSSLSKDLPEDPDLFY
                     LAATESLKGCVKSLSGQGRYLRGVFPEEMKEIRETVDQIGREMNAMTPAIAEARKQRE
                     VLSAVRIDLSRLNSAEKEKKHTADEINLLRGETLRETGDIDRLCDRIRAVNEAIDQGS
                     LRNQQEELATAKREFLEKEMALQADLAVVAHVFRKAEKVLGRTIGTASVKDIESFVDT
                     LAGSGIPAEEQIVPDLIRLLPTLASMRDSGDLVLKNKEEKELFSREMDLPSRIRDGYA
                     RREVAYRSFHTKEHAYQENPLLVELSALQREADRRKGHAESLTSRLSSLMEKNAALEK
                     EIPDLEERVRSDVGRLLGHAVSIAMDDATELSR"
     gene            18281..18547
                     /locus_tag="PK154_00590"
     CDS             18281..18547
                     /locus_tag="PK154_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09590.1"
                     /translation="MPDDRYRLLEVTDGKVPCLPGKNETIEHCRFCVHSRFFRVRGEY
                     VKSPALAYCLLHRATKEVDLKSVDAVQCADRDGEGYRSMMNIIG"
     gene            complement(18551..18892)
                     /locus_tag="PK154_00595"
     CDS             complement(18551..18892)
                     /locus_tag="PK154_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPW09591.1"
                     /translation="MVLRISITTDTEMMELIDKYAKENGIDRNRAIIEMIENGFTKAT
                     GTTPFSPNQKQTFEEYHDIKLSLETLKQSLMELTDEVKLIHHIIDVELVREMKPVPFQ
                     SKKWWEFWKTP"
     gene            complement(18960..20405)
                     /locus_tag="PK154_00600"
     CDS             complement(18960..20405)
                     /locus_tag="PK154_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HPW09592.1"
                     /translation="MSLLVIGTASHVGKSTVVAALCRILTNRGIRVAPFKSQNMSLNS
                     FITSDGAEIGIAQAMQALAAQVEPDADMNPILLKPKGEMVSQVVLKGRPWKDVRISEY
                     YQETDYLLSIAVASAHRLLEKFGHIVAEGAGGAAEINLYSRDIANIRIERELRLPVLL
                     VADIERGGVFAQIYGTLSLLPEDIRPRVMGIIINKFRGDPALFEEGVQIIEDLCGIPV
                     LGILPVFSIPLPSEDSLSLGDKRSCNLPVKIGIIRLPRISNFTDFERLEPYAAVDYLH
                     PGEPLGGYDCIIIPGTKNTVEDLLLLQASGTAADIVRASQQGIPIIGICGGYQMLGKE
                     IFDEGLESRCGRYEGIGLLDVTTRFSRYEKVTKQVHREALPHGPILAQMGSVKGYEIH
                     MGETIRTGDNEAFEGEGAVSEDGLVIGTYLHGLFENPAAVRALLTYLYRKKNLDFEMD
                     ATEKDRDVYEDLAAEFERHVRLDPILAFFCD"
     gene            20461..22113
                     /locus_tag="PK154_00605"
     CDS             20461..22113
                     /locus_tag="PK154_00605"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPW09593.1"
                     /translation="MALSEAGKILLESRYLLSGETVSDMFQRVARAVNTGREAQFCQI
                     MEDLLFLPNSPTLMNAGTPKGQLSACFVLPVEDSIQGIFTALTQMAMIHKSGGGTGFS
                     FSRIRPAGDIVGTTAGVASGPLSFIRVFDEATTALKQGGRRRGANMGVLASSHPDVLS
                     FIRAKKNGDLKNFNLSVGIDRHFFDSYCRKGTYDLINPRNGEVSRTIDTGILWEELCS
                     AAWQCGDPGILFLDRINDLNPVPGLGPIEATNPCGEQPLLPYESCNLGSLNIAGFVRK
                     GELDEDKLVSVVRFGVEFLDAVIDINTYPVPEIRDRTLLTRKIGLGIMGLADALIMME
                     IPYASNEGLNVTESIMQVIQQEAHFASEELGEKKGSFPAIDKSWYPGPMRNATVTTVA
                     PTGSLHIIAGTSSGIEPLFSLAFSRIINGKAIRFVHPAVRHIVESLPRGQTLMEQIER
                     TGSVQNLPIRDYEKELLRTAPEIKPEFHVRMQAVVQKNVDNAVSKTVNLPEHATLEEI
                     SGIFLLARTLGCKGITVYRYNSREDQVLSRGCDTCRVDGLGS"
     gene            22236..22541
                     /gene="yciH"
                     /locus_tag="PK154_00610"
     CDS             22236..22541
                     /gene="yciH"
                     /locus_tag="PK154_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HPW09594.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRINVKVNKRRYGKEVTVI
                     EGLDPTDIDLEDLSKYLKGKLACGGTVKESSIELQGNHKDRVKNLLANKGYSVENIS"
     gene            complement(23128..23508)
                     /locus_tag="PK154_00615"
     CDS             complement(23128..23508)
                     /locus_tag="PK154_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HPW09595.1"
                     /translation="MVHTCAKCGKVYPDSSAEVLVGCSQCGGKKFYFERPNARLKKKH
                     ESSITPKAPTSTPPPLPEKHKIEEITGSCVESIRIVAPGTYELNIEKMAQCDDRVVGL
                     GAEGSYAVDLLSMSGAKKKKKTDR"
     gene            complement(23513..23878)
                     /locus_tag="PK154_00620"
     CDS             complement(23513..23878)
                     /locus_tag="PK154_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HPW09596.1"
                     /translation="MQQGVQIDLISEERLSKLTSMEKIHMILDDVRQGTIVILEKGLD
                     PEEQGTLIEMTMHEILPDGFNGIEIETYPSHEEAPGFLQRLLGRTRPESRLTVIGPAN
                     QLRMIRKDKDLISAWVSSR"
     gene            complement(23885..24526)
                     /locus_tag="PK154_00625"
     CDS             complement(23885..24526)
                     /locus_tag="PK154_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HPW09597.1"
                     /translation="MSIFVQAKNRVSKFLMAFFKKKRSRIGIYGPPNAGKTTLANRIA
                     RDWTGEVVGPVSEIPHETRRATRKQDITITGYNGSTITIDIVDTPGVTTKIDYKEFLE
                     YGVGKEEAITRAREATEGVAEAMHWLREDIDGVIYMLDSTEDPFLQVNIMMVGIIESR
                     KLPVLIVANKIDLPDASPQRIRTAFPQHPVIPISSTEGSNIEELYEKMVLHFG"
     gene            24766..25332
                     /locus_tag="PK154_00630"
     CDS             24766..25332
                     /locus_tag="PK154_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW09598.1"
                     /translation="MKKTSDSIRFETRVPLSEVMKGNPTTIQSDASVARAAMAMCRDE
                     VGSCIVLRDDLPIGIVTEEDINCKVVAKDKRPSAVLVNEVMSTPLITIRSDKTVRDAA
                     HMMIRNRVRRLPVVDDENRVIGIVTVRDILTVSTEINELMNDLIEINRLEEIEVGTCS
                     RCGQMSDDLRRIDNIMLCTSCREEELLQ"
     gene            25329..26075
                     /locus_tag="PK154_00635"
     CDS             25329..26075
                     /locus_tag="PK154_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW09599.1"
                     /translation="MKTAADFMVEFPELKSDDRITKARKILRENTFREVYAHDGKRKL
                     AGYIDITDVLHVTATKSDVTIEGYLKDITPVSQGDTLGIVLETIRKNKTDSVPVVDDH
                     GIFLGGVLLSELFPVVLTMQRLHGPVCDSMTRNVISCSAEDTVQRIHALIIDTGYSAF
                     PVIKKKKVIGIISRRDLLKDGRWRTSSETTTTVDTLMTTPVITAGPDDDIRAAADCMV
                     KHDISRLPVIENDVIVGIIDRHDVLNSMAR"
     gene            26205..27098
                     /locus_tag="PK154_00640"
     CDS             26205..27098
                     /locus_tag="PK154_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW09600.1"
                     /translation="MPGKLDRGPVEFKSHIVTREGEIMAIATRDVVSVPPTMPILAAV
                     ETMTNCGFRRLPVVDAGSRKLRGIVTSGDIINFMGGGDKFNLVRIKHDGNLLSAINEP
                     VRSIMTQQMTTLPDSAAINDAIDIIVNRKVGGMPIVDNDGVLVGIVTERDVMKVLATE
                     RSGLCVNDIMSTALRITNPDCPIRIVTRDMTTNRFRRLPVVSNDVVYGIITTTDIMKY
                     LGTKKVFSQLKTGDIDEVMDLAVRNLVSGNLFTISPERSINEAAREMVDRNVGALPVI
                     ENTRLVGLVTEFDLVKALSRK"
     gene            27106..>27313
                     /locus_tag="PK154_00645"
     CDS             27106..>27313
                     /locus_tag="PK154_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW09601.1"
                     /translation="MNVGDVMSSPVFVIDSGENVARARNLMLKHRISRLPVLEKSLLA
                     GIITKKDIGYRLRQSEPLWRRRPID"
BASE COUNT         7067 a         6464 c         6815 g         6967 t
ORIGIN      
        1 tcttggctga tgggaaaggg gaactgtatg gtgacaaggc aatctacctc atagagccga
       61 gtgaccagtt tactctttat cgtctcatac caccattggt cgaaaggatt attttaacct
      121 gcaggattta tgataaaagt catcttatct gtcaccaatc gcttgccccg gttgaattcg
      181 gaaaaaaggc agagggtgtt ggaagggctg tcattgcgtt aaaaaaagac ggagttgttt
      241 ttcccggtct ttctgtgcgt atccgcaagg atggtcagat cgtcgccaat gatatttcca
      301 atgcgaatgg ggtcgtttcc ttccgtcttc tttatggaaa ttatgaggtt ctcattgtaa
      361 gatcagagaa taacattgat atttttgatt tctcgtttat ccctgatctc caggggcgcc
      421 aattggacct tgaattaccg taatcccccc cgcaactgca tttatttcaa agacaatcaa
      481 gattattttt cgacaagtga tagttgagcg gttttgcggt catggaatca atccgttcgt
      541 atagctccca ccgacattcc gcagatctct taaccaagct aaacattttt agctaacttc
      601 tgcataacta ctaaggcaga atgagtgtct cgtctgccat cgtggcccac catggaatag
      661 ttgctggtat ctttgatgaa ttggagatag ggcgaataat cgatgaaaca atccctaaac
      721 aaggccagca caagctaccg cattctctgg tcctcaaagc catggtcctg atttctctag
      781 gattcaatga gaggagactg tatatcttcc ccaaattctt caacaatatc gcgacggaac
      841 ggttacttgg accaggtatt cttcccgaag atctcaacga tgatgttctc ggcagaacat
      901 tggaccggat ttacgaggca gactcaactg aactcttcct taaaattgcc ttacaggtca
      961 tgcagcatct cccgttcggc acccaactcc tccatgccga cactaccagt gtaagtgttc
     1021 acggcaacta tgagcacatc gacggaactc cggcaatcca gatcacctat ggccatacca
     1081 aggataaccg tccagacctc aaacagtttg tcctcagcac catcaccaac cagcacggga
     1141 tcccactctt tgcgaagacc tattcaggga atgcctcgga taaacagaac ctgatcgatt
     1201 caattcaaca attacgagcg gtggtacaga ctcctgatga tgcatatttc atcgccgata
     1261 gtgcgctcta tactgcagat aacatcagga acctgggaca gaatattcat tggatcaccc
     1321 atgttccgtc taccattacc gaggtaaaga cgctactcca cgccgatgtt ctcatgcatc
     1381 cgggcaagga tacccggtat tcattttacg agacaacttc cgaatatggt ggcattcgcc
     1441 agaaatgggt actggttcat tcaaaagaga tgcaggccag gaaggagcag accttcgaaa
     1501 ccagcctgga aaaactggat gtgaagactg caaaatcgtt caagaaactc ctgggacttg
     1561 aatttgcctg tgagaacgat gcacgggcag cagccgaaca ctgggcgaag aaccatccgt
     1621 ggtaccagtt cacggggttc acgattgtcc caacctcgcg aaagatcgaa gtgaaacgag
     1681 ggagacccag gaacgatgag gaacgatgta ccgtctacac cgttcaggct gaaatcgtgc
     1741 gaaacaccga ggtgatccat gcagagaaag agaaactggg gaggttcatc ctggcttcaa
     1801 atgagctctc gcttgaccct gaaacgatgc tagactatta caagaaccag agtgcggtag
     1861 agaaaggatt ccgatttctg aaggacaaga gtttccggat ctccgaagtg tatttgaaga
     1921 aacctgaaag gattgaggcg ctttccatgg tcatggtgct tactcttctc atctattcgg
     1981 tggcggaatg gctgattcgg acacggttga aggaacggaa tgaagttgtg cctaaccagg
     2041 tgaataaacc gacgcaaaat cccacattga aatggatatt catggagttc atgggggtta
     2101 ttgaagtgag gatgaatgtg cctggtcagc cggaacgaca attgaccaat ttaaacgaga
     2161 atgcgagaaa aataatcgcc attctggggg ggaggtgtga aaattattac atatgagaaa
     2221 agatctgcgg aatgcgggct cccacaatca gattgcaatg aaaatggcac gccgtcttat
     2281 cttcattgaa tctctgaata ttcacccgtt tgatgtttgt tattcaaatt aattattttt
     2341 actcgttctt atgcatttta ggcagattaa tgggccccga ttcctattcc ggacctgtat
     2401 tatttaaata ggttcaatcc aagatcagat tcatggatag gaaaaagatc agcaaagaag
     2461 attttgagtc cataatcgcc aataaaaaaa atgttcagga atatctggaa acagatccgg
     2521 aactgggaga gacattctgt ttcggcgtcc gtaccgcaag taatcccgat tacctgacaa
     2581 aggctatgta tgagatgtat gaaacaagcc tttccaggaa acttgcaatc caggcagtgc
     2641 gttctctcta tcgttctgtc gggcttggtt ctgtaggact caacaattac ctgaaaaacc
     2701 tggggataca cctttctcca gccgaattcc tccttcttgt cttcaaactt cagcaccagc
     2761 aggggtgggg agcgccgttt gagcttatcg aacagaagga aaagagaatc attctccaaa
     2821 caagtaagac cttcgaatcc caggtgatga aggagtggaa gatgccggtt tgcggtattc
     2881 acaggggatg gattgaggga gtattgaccg cagttaccgg gaagaactgg ttctgtctcg
     2941 agactaagtg ttacgcacag ggggatgact gttgccagtt cgtggccgac cagacagaat
     3001 cgagctggaa atggaaagca caagcgattg tgaagggaga gtcggcaata actgagtata
     3061 tcgaccataa accccttgaa gggaagatca aactaatcga tgatccggta gtcatgatgc
     3121 cccggttcat cttttcttct atgacatcat ccctctcaaa aatcatgggg gaagttcctg
     3181 cggggggggt gaattaccgg gcatacatgg acatggggaa agagaatgtg gagcattata
     3241 agaaaatggg ggtcaccaat ccgaataccc tgtcagatat ggcatttgca ttctattcgc
     3301 aaatgggctg gttccggatc gtgaagatgg actggaatga atctgaaaag accaaaacga
     3361 ttactcttga gcataccgtg gaatcagaat ctatggggaa cactgggaag agcgtctgtt
     3421 attgcacggc aggtctcctc gcagggatta tcgagggtgc atttggaatc aaggtgcggg
     3481 gacgggagat caagtgccgt tcaaaagggg acgaacattg tgtgtttgaa ataagaaacc
     3541 gatcgggtga aagttaatga ttgttctcct ggtttaatgc cataagaaga agcgaggtcg
     3601 tcctgttcaa atgcagatat gttctctctt ttttatttcc ttcccctgaa gatcactttt
     3661 gtaaacctgt caacaaacga gtcttttgaa tccatttctt tttgctcata tgtcaggcct
     3721 gcaagtcctg cagcaagctt ttttattgcc acagaagaat cggatgcagg gtatttcagt
     3781 acaataggaa cttttgctga agcggctttc ctgatattct ggtcttcagg aatgattccg
     3841 attactcggg tgccaaggac tttttccatt ttctgggtaa ccgtcgcgat gctttcaggt
     3901 gtagcacggt tgatgattgc gccacggacc tgtccaccta ctacttcagc caggattttg
     3961 gtcttcatgg catcgacgat agctgataac tcgggattca ccacaaggat tacctcatcg
     4021 gcgacggcaa gcggtactac accgtcacga cttattcctg cgggggcatc aataagaaga
     4081 taatccagag aatccgtgag ctccttcagg acttctttaa gaagttctgg atctgattgc
     4141 tgaaagcctt gaagagatat cccgcatggt ataacctgaa ccccgcccgg cccctcatat
     4201 attgcgtctt ttattgaggc tgttcccgaa agcacttcat gaagggtgac ggggacattt
     4261 tcaagaccaa gggagagacc gagattggcc ataccgatat cagcatccat gatacaggtc
     4321 ttcttcccaa actgtgcgag gctggtcccg aggttgatag taacggtggt ctttccggta
     4381 cctcctttcc cggatgctaa cgtaaaaacc ctgaccatta agcccttcct atcaattcat
     4441 cacccacata ataaataaag tatggtgcaa gggatgggac gtgactgcta acaattctgt
     4501 tcaaagaccg attgcatctc cttggcaatg atgaccatcg tttcttcagg gataacctcc
     4561 ctggtccgtg cgatcccgac aagaggaaca cccctgtggt tcagagggac gagcaagatc
     4621 ccctgttcgg gttgggcaga tttcccctca aatacactgc tataataggc agcatcatat
     4681 tcaggatttt tattgcccac ggatgcgaca acaagcccat cagtcatggc aacgaccatt
     4741 gaatcaatgg tatatctccc aataactgac tgcatccctg agacgaatgt gccattattc
     4801 tcctgaagag cgttctcggt atacttgttc ccctgttctc tcacggccgt atctttctga
     4861 acagataacg gagatacctc attttgggaa tgagaaattc cgatgttttt caagaccgaa
     4921 atttttttcg acatatcgat gagaattagg gcattgaaga ggctgagaaa gaggctgacc
     4981 gaagcaatga ctagcgcaat tattaaaagt cccaattcaa aggaaatcgg attcatgggc
     5041 atcatttctt ggaattttct tcaatgaggt gatcgagatc tagtcgttcc atgatctgaa
     5101 tgcaactggt tcggaatttg gtcgtcatgc tctcaagatc catcgattcg atgagatcca
     5161 ggtcgtggga aagcagatct ccctgagacc atgccgtcga ctgatcattc ttccgggata
     5221 tttcagctgg ttgaactcca ggtcctgttc cagaggaaaa gggaaaggga gccgcccttt
     5281 cccgcgcatc atgggaactt ttaccggagg ttttgatgcg gattgcgtca cttggccgaa
     5341 tgcgtccttc attgaaaacc tcctgcatat gacgtttttg agtcttattt ttcacgaatg
     5401 ttttgaaaga gggattaaat tctaacgcga ggcgaagttg tgcatacgtg agatcatgaa
     5461 gaattgcatt gctgttccaa tgtgaggcag ccacaatctt ttcaagtgct tcatctcctt
     5521 caagagaacc gaattcagct agccggatac gtcctttgtc gcatacaaat tgtgtcatct
     5581 cattatcatg caggctctgg cagtatcctg tgaatctgca attgttcagt ttctgaacca
     5641 gggcaggcag gttacaacca cgtattattg tcctgaaagt gcctctggga atctgcattc
     5701 ttgtcaccac catatggagt ccccccatag gacggaaatt tctttgacag gcctgaaacc
     5761 taccggaacc aggcatttat tcccctggga ttcttatggt aaaatcagta tctgtattct
     5821 gattaatcgg ttcgatgaat cctcgtaatg gtgatatata tttgatgatc cagagtatgc
     5881 tcttttcaaa caggttactt ataaggtggc atgaatgttg aaacagattt taggtgagtt
     5941 tctgaatctt gaaggtgtct ctgcggcagt cgtcgtaggg cgtgatggat tcgtgatcga
     6001 gagtgcggtg tccggaaaaa tggatatcga tgcacttggc gcaatgacat cgacagggat
     6061 aggtacatcc gaagcgatgg gaaaagaact ggggaagggg cagctcaacc agctgctggt
     6121 agagctcgac aaagggccga ttattgtatc gcccctttca aaagacgaac ttattgcaat
     6181 tgtggcagaa aacgcctcaa atctggggcg tatccgatac gaactcaaga agaataagga
     6241 gcgcctggtc gcggcgctgt gagtgtcgga ggctcaacga atagaatgga tacatcattc
     6301 catggtggta tcattcggat ggatctctga tctccatgga taaatggggg acctccgatg
     6361 agaagggttc acaccttctt gatgatagcc gacttgcggc tgttcagaaa cagcagggag
     6421 tccttaccgt actcgcattc catgaaggat ttccggtaaa gtctgcggga actggtgatt
     6481 atgagcaggt tgcagctgtt gctgaggatt ttttaagggc gggaagaaag atggcacgag
     6541 agatgaaact gggggacatt acccagatca ttcttgaatc agatgaaaga aagtgtatta
     6601 tcgctcctta tggggatctc ttcctctgcc tgttaaccag atcagatgtt catctgggtc
     6661 ttctccggct catcataagg gacctgcagg acatggattg atctggatag gattgtcggt
     6721 cgaacatgaa cgtgaggtgg cgagatggca gtaaaggaat ctgaaaaaac atcatcatta
     6781 aaaaattttt tctccatcgc aaaaaaagga gaagaaacgg aaaaaggcaa aaatgaagga
     6841 gcttcaaacg atttcaagga aacggatgaa gatagtaaat tcttcgagaa cataataact
     6901 gcaggtgaag ttccggaaaa caatgccgat aatcctgatt tttctgaaga caccagcatc
     6961 agaaaaagtg taatacttcc tggtcattcc tcgatgaatg ggatgcagag tgaatcacca
     7021 ggtgtggaat ctgttgttgg tggcagaccg gttgtcaaga atagcatatc ctctgcccgg
     7081 tccgaaccga tcagaaaaag cattagttca gcagagcctc cagaaggaga ggatgcagtc
     7141 gtctctatca atgagttcga tggtatatcc ggccttatac tcccaaagag tgctacattc
     7201 aaagtagacg aactaaaaat tcaatcgcgt ccgcatatct tcgacctgaa aagttcaggg
     7261 ggaatacctg caaagaagga ggattacctg aaaggcgaag gtatcaggtc aatttctgaa
     7321 gctgctgctt cgatggagac gtcactgcca aaaaaaagca ttcttgcaag actgaatccc
     7381 cttaccaccc tccgtcccgt catcacagaa tacgatccaa acctgcatgg accactggtt
     7441 gacctcacgt atcagcccag acctggcatc gaagagatag aactctatcc cgtaaatgag
     7501 ccgtatgcct atatcaggat cacctatgat cataccaccc atgaaaatac gtacgggatt
     7561 ctggaaccga ttctcaccaa ggccgaggaa gaattgttca gcgagatcaa agaacgaatc
     7621 tttgaacggc tcgatatcaa tacccaggac atgcctgatg agcaggcaag gacaacgctt
     7681 cgtaatgctt cagatgaggt aatcggggac tttggtatcc ctctcactcc tgtgcagcgg
     7741 gagaagatac tctatagtat gtataaggag tttctgggaa atgggatgat agatccgatc
     7801 atgcatgacc ggtatattga ggatatctcc tgtgacgggg tgaatgttta cctctttgtt
     7861 taccataccc gatacgaatc gatgcgtacc aatctccggt ataaaactgc tgaagaactg
     7921 gattctttcg tgacaaaact tgcccagcgt tcaggaaaat acatttcaat tgcagagccc
     7981 atgctcgatt caaccatgca tgacggttcc cgtatccaga tgacgcttgg aacggaagta
     8041 actgctcacg gttcaacgta tacaatccgt aaattcaaat cagaacccat cactccaacc
     8101 gatcttatcg aatggtctac cttctctcct ttgtctatcg catttctctg gctcgctgta
     8161 gaagccggaa aatcatgcat ctttgccggt ggaactgctt caggaaagac aacctcgctc
     8221 aatgccatct cgctatttat tccccccctt gccaagatag tgacacttga agacaccagg
     8281 gagctgaaac tgccgcatcc caactggata cccagcgtca cacgcacctc ttttgataca
     8341 gaagggaagg gcgagatcga tatgtatgag ctgctcaagg cggctctccg ccaaaggcct
     8401 gagtatctca tcgtaggtga ggtgaggggg agggaagccc tgacgctctt ccaggcaatg
     8461 agtactggtc atgtaactta ttccaccctg catgctgatt cagtagcaag tatcgttcac
     8521 cgtttagaaa atccccctct tgatgtacca cgcaacatgc tttcagcact ccatcttgta
     8581 tcggtgcagg tgcaggcacg catcggaggc cagcgtatca ggagaaataa gcagcttgtg
     8641 gaaatcctcg atatcgatcc taggacaaac gagctcataa ccaatgatgt attccgttgg
     8701 catcctgtca cggacgagat tacctactca ggaaaatcgt tcctccttga agagatcatg
     8761 gaatcccgtg gctggactga gagcaggatg agggaagaac tcaagatgcg gcaggaggtg
     8821 ctcgaatgga tgagggttaa aaaaatccgt gaataccggg acgtgtccaa catacttatc
     8881 cagtattacc gggatccgac aaaaataatg gagaaagtgc gagctgagct gtatgaatag
     8941 ctttcagcgc ctcgccttcc ggatactcgg aaaatatgcc gggagaaagc gggacaaata
     9001 ttcagatctc cggaattcgc ttcttacggc ccggatgaag atcccttttg aggtgtacct
     9061 ctcaacggca tatcttgctt cttcagtcat tggtatagta agtgcaattc ttctgggcac
     9121 agtcacctgg ttattcaatg tcccgggaat gatcacctac cagggagaag tccctgaggt
     9181 ccttctctcg cttaaccaat attccctgat aatcgggact cttattgcaa caattgtctc
     9241 atttgccatt gttgccggta ttattttcct cttgtttctt ctctatccgg gatttgtttc
     9301 agggaatcgg aaacggaata tcgaatcaac cctcccgtat gcaatcaatt atgtgacagc
     9361 gatgtctact gcaggtattc ctcctgcgga aattttcagg cagcttggca gcagtacaat
     9421 atacggagaa agtgctactg aggcccgttt tattgcactc gaaattgatc tctttggtcg
     9481 ggatctgatc gatgcccttc gcgtggtctc atcgactaca ccgagtttca ggatgaaaga
     9541 atttttacag ggctcaatgg ggtgcatttc tgccggcagt aaccttaccg aatacttcag
     9601 aaacaaagca gagcagtatg ctctggaaaa tcggcaaacg caaaagatgt tcctcgaaac
     9661 attggggctg atcgctgaat cctatgtcac ggccatggtg gcagggcccc tttttctgat
     9721 aattctacaa tcgatcatgt cgatcctctc cagccagtcg cagccattca tgctgtacat
     9781 tattatttac ctcatcattc cattcggaag catcgcgttt gtgatcctta tcagtacgat
     9841 gacgccggag tcctgattat ggcgatgaaa gggttctttt ccgggaaaaa agggttgaag
     9901 ggatcagggg agccggatcc tttgatgcca gtagatcccg attcaatggg caggatggga
     9961 aaactctcgt ttcttctctc aagggggact cccgcctctg tcgatctggg ggaacgagag
    10021 aagttcgagt tcgaacagga aagcagtgcc atcatatcac ggatcgagaa gtataaggca
    10081 actgaatttg gaattgggcg actgttgaag catccattcc gggtaatcac agaaaaaccg
    10141 ctcaaaagtc tgtttgtgac cgtcccggtt gcactggcat tcttctttat cggtacggca
    10201 attgtgatag agaatgaagg aatcaatgcc ctttttacct ctaccgcaat cgatgatctg
    10261 gcagtattcg ctttacttat ggtctcgatc ccactcgcga tactcgattt tcgggattcg
    10321 tggcgtacac ggcacatcga agaagcactc ccgaattttt accgggacct tgccgggatg
    10381 aatgattcgg gcatgactct gcccaatgca gttcatcttg ttgctcagag tgattacagt
    10441 acactcactc cttatgtaag gaaactcgac aatgaaatgt cgtggaatac caccttcatc
    10501 gatgcaattc agcggttcag agcccgattg tccacgccac tcaccgatcg gagcatcgat
    10561 cttattgcaa aagcgagtaa agccggggga gatatcagcg aggtgctcag ggctgcagcg
    10621 aaagacagct acgagttcat caatctgcag acggagagga ggaacaatat gctgatctac
    10681 gtagtgattg tctttatctc gtttctcgtc ttcgtctttg ttatttatat ccttgtcact
    10741 accttcctga gtgtcatggc gacggcgggg agtgctgcca gcgcatccgg agctggatcc
    10801 cagtttggcg cgaatgtcaa tctcccgctc tacacaagga tttttaccca tgctgccctt
    10861 attcaggcat tcttttcagg cctggtagct ggccagatgg gtgaggggag agtgattgca
    10921 ggacttaaat attcgatcgt gatgatcatt attgcctgga tcatgttcag gttctttgtg
    10981 tgaatacgat catcacccca ttttcctgaa gaagtctggc agggttggtc catacacaca
    11041 aaccagtagg gggtgctgta ccaaggcggc cttccgttcg tgtaccgtaa ttagatgaaa
    11101 ggtgaccgga ttctttattt gaaaaaaaat gttcaaatga ttcccatgtc agagagtcgg
    11161 gcgggcagat actcctttgt cacgaaatca agtcctttcg ctgcaaatgc ctgctgttct
    11221 gatttgagat tgagtttaag ctgaagttct atctgttctt tccagtaatc ggaagcgaac
    11281 cttggatcag tcaattcact cctgagggct ccgatgtcct tgtcgttcag atgatcagaa
    11341 ggcaggtgat agtccctgat atcagtcggc tggacaccga tgaatcttgc tttgggggtt
    11401 gcaagcagtt cagagatatg ggcacttttg attgcaccat acgcaacact tgcgtatatt
    11461 cgataggacc aggggtctcc atcagtaaac accactaccg gaagattgaa ctgcgtgttg
    11521 atccggttca ggaaacgcct ggttgatcgt gctggctgac ctttgaggtg gatgagaatt
    11581 gcctggtgat ctccgtcaaa tccgttttcg atcagtcttg catacatacc tccggtctct
    11641 attgcgatca caaacttggc gtcgtgttct agcaattcaa ctgtctcgac attggtaggg
    11701 atctggtagc ctgcttcccc aacatcatcc tggcaatgga tgtccctctc tccacgccgg
    11761 gtcatctccc gcagttttaa aggcccgaat atggttgctc cgtcctcttc agggcgaaga
    11821 tggaatgctt ccctttgcat atcggtgaga atttccaaat cctctatcag gaaattactc
    11881 tcatcctgag cctggaattt cgctttcttc cagccttccg agatataata cagttctctc
    11941 agagtcgatg aacggttttc accgatctgt tttcgcaaaa accagatgac atatgccatc
    12001 ttcagcaggt ggagtgcact ctttgccgta cctgctgagc ggatactctc ccggtcccca
    12061 tatttccaga cttcggatgt atcatcatat tcaatattct gttttgttcg tgtcggaaga
    12121 gcgatggaag ggatctgtcc ctcaagcatc tggtcatacc atttccgagc gatgccgatg
    12181 agccgcccgg tagccagaat atcaagatct tctttactca tctcggtcca ccataacaac
    12241 attcctcgga tcaatgccac ggatttcgac tgtcccatgg gctgatcctg aataggttgt
    12301 cgtccacact tttcctggat caaccaccac ctgccagacc cgggtgaaat cttcatccat
    12361 ttttgtcaaa aaatccgggg ggggatcagc gttattttcc gaatccctgg tgatggtata
    12421 aagagagagg gatgcctggc ctgcggtgta attttttatc tctatcctaa caataccctc
    12481 tgataccgtc ttcctcacaa taagcttatg catgatcctg ccctcaatcg atgaaatgtc
    12541 gggtttaggc agctcaacta tctctgcaac cttttcggcc agttcaggaa ggatagcaca
    12601 cactgccctg gcacgatctt cctgcaaccg gttccggtct cttcgggaga ggaagacctt
    12661 caggtcgcgg gcgagatcct gcagtccaag ggtgatctcc cgctcaatct cagggatgga
    12721 ggcaatggca tccttactct cgctggtaaa cggaacactg gtcgatgcca catgcacaag
    12781 aatgaggagg ggtccggtag ggagacctgc ctgggcgaca gcatagttct tccagttcat
    12841 cgcagaaatt gcagaagtga ttgcgcatgc tccctgctgg tacatcagag gtacccggtt
    12901 tgcaaaccga agaatctgtg cattcccgtc ggccgcaagt tttccaccat atcctatcgc
    12961 ggcttctatg atgaacgggt gccctgaaaa gaccgatcct ggccgcgtcc gggcttttac
    13021 gaagtccatc tggaattctt tttcaagacc ccttatgata aggtcctctc cgataggcga
    13081 aagacactgg gagattggag gggggggtac tttcaccatc tgcatcgcct caataagccg
    13141 tttctgttct ttggcagcga gagatgatgc atcagctgtg caggacagct gtgcaatccg
    13201 gcatatctct tctgcagttt tcttcccgat tttcgagaaa ctgctcataa gaaacgattc
    13261 aagtgttcca ggagatgcct gaagcatcct cctgatctgc ccgacctcga ttccgtgggg
    13321 atggggttta atcgccctgg gacaagcgat cacctcatcg ctgacccggt cgaacatgaa
    13381 cacctcgcca tctatttcaa gccggatccg ggcatgcgga tttaccacgg aggtgtatct
    13441 cagatattca ataagccgtt ttttcgcggc aagggtgctt ttgaactcaa tctgaatccg
    13501 cgtcccatgg gtccgatccc actctatctc gcggcgggta aggacttcgg gttcattcgt
    13561 atcaatattg atctgaatgg cacattcatg ggcagggtat tcagggccgg tcctggaaac
    13621 gatgatggtg gggacccctg ctgtgagctg ggcatacagc actgcagcgc tgataccgat
    13681 cccctgctgc cctctgctct gtcgtatctg gtgaaaccgg gaaccataca ggagcttgcc
    13741 aaagatgaag gggatctggt ccggaatgat cccagggccg ttgtcttcga taacgatccg
    13801 gtagatgtcc cggtcctgcc tgcttactga gagaaatata tcgggaagct ccatcgcctc
    13861 ttcgcaggca tcaagagcat tatccaccgc ttccttgatg gtggtgatga ttcctcgtgt
    13921 gggcgaatca aagccaagga ggtgcttgtt ccgctcaaaa aattcggcga cgctgatgct
    13981 cctctgctgc ttagccagct cctcagcgag gctcactccc ggtcacctca ctgattgctt
    14041 cggggagagt gagttcttgc tgtatcccgg atctgagatt tttaagggtc accaatgaat
    14101 tttccttctc tctctttccg ataatcaaag cataatcggc ggatttagac agtcgggaca
    14161 actgctgggt cagggaccgt tcagagaggt cgaactcggt ccggatcccc gccgccctga
    14221 attcgcgggc aaccgcaagg gcccaatccc tcccatcagg ggtataggca atcccggcca
    14281 taatctggtg cggagggcct tggtcccctc ttgcgaccat cacccgatca aatccaatgg
    14341 caaatccgca ggagggcgta tcttctccac cgaaaagatg agcaagacga tacgttcccc
    14401 caccaagtac ctggttttca gttccaagat tttgggcgaa tatttcaaag accattccgg
    14461 tatagtaatc gagtcctcgt gcaatgccgg gatccagggt atacggaatt ccggtcgtat
    14521 caaggatcga gaagagttct ccgatcctcg ccttttccgg aagatctcct gtgatcgcga
    14581 acacatcgtt caggtttcgg caggatgtta attgtatcag cgatgtgcag aggtcatccc
    14641 atcctttgct ggtgaggtaa tctgaaaggc cgtcaaaatt tttcttgtcg aggaacatac
    14701 ggattctttt ctgttcctgt tccgggatct ctgacagcag gctccttact aatgccagat
    14761 ggccgatatg aattgagaat gagatccccg tagatgagag tatctcttca gcaagcatga
    14821 tgacttctgc ctctgcaaga gcagaatcca cgcctataag ttccacccca aactgccaga
    14881 actggcggta tctgcccttt tgtgggcgct catagcggaa acaatcggca aagtaatacc
    14941 agcgaagcgg ttttggcaga gttttccctt cattgacgta catccgaaga accggcgccg
    15001 taagctcggg gcggagggcc atcggcctcc cccctttatc ctcgaaaacg tacatctcct
    15061 ggatgattcc ttctcccgaa cggatggtga aaaggtcgag ttcttcaaat tcgggggtgc
    15121 agatctcacg gtagccccat ctccgtgcca cctcgcgcat cctgccttcg atctcgcgcc
    15181 tctgttccat ctcgtcaggg aggaaatccc tggtcccacg tggtttttgg agcatatcgc
    15241 atcaagcctc cttatttttt atcccttttc ggagaggggc tttcccaaga aaccggtcga
    15301 gggtttcctc tgacccccat atcttttcgc gctcttctct cccctgaaga taaagggcag
    15361 cgaggataag gccgagcccg aggagttcca gcgaatatat ggttactgct acaccaagaa
    15421 tggccagagc accaaataga attcccctgt atgcagcctt ccggtatcct ggaagaccgg
    15481 tccgataaaa aatcctggca tcacgcagcc acaggaaaaa aacaactgca gccccaaaga
    15541 gggcgatata gacgagataa ctcatgatta tgaacgtatt attatacgcg gacccatatc
    15601 tttattattg aggtatgcag gcaaacagaa agctccgtga agctctcctc cgattccagg
    15661 aaggcgccat cagtctggaa gagacggaac gggagattga cggattacgt attgaggaag
    15721 tctccgatat cgcctgtctt gatatcggcc gaaatatccg ctgtggtata ccggaggttg
    15781 ttctcgccga gggaaaagag tcagctcacc ttgcagatat tgcgcttgcc catgcccgat
    15841 catctggcag atgcgtcata tcccggttaa accgggccca ggaaaagata cttcgggaac
    15901 gtgcagaaaa atcagggttt actctgacct gccatgagac tgcgcggatg gcggtgctgt
    15961 ctactggaga tccgatcaca cttaccggcg gaatcattgc tgtcattact gccgggacct
    16021 ccgatatacc ggtcgcagaa gaggcacgcg tgattgcaac tgaaatgggc tgccaggttt
    16081 gtaccgcata tgatgttggc gctgcaggga ttcaccgcct ttttcccgcc atcaggaaat
    16141 gtcagggtgc acaggtcttc gtcgttgcag ccggccggga ggggaccctc ccggcagttg
    16201 ttgcagggat ggttgaccgg ccggtaatcg gggttccggt cagcacaggg tatggatata
    16261 tggggaaagg gaaagctgcg ctggccagca tgctccagtc ctgttccgtg ctgacagtgg
    16321 tgaatatcga tgcgggattt acggcaggag ccatggctgc cttgatcgca aatatggtgg
    16381 taggccaatg acacgggcat tttatatcgg gcgatttcag ccttatcaca acgggcatca
    16441 gtatgtcctc gaaaaaatcg caggaacagc cgatgcgatt gtgatagggg tgggaagtgc
    16501 acagctcagc catgagaagg aaaatccctt tactgcgggg gagcggattc taatgatcac
    16561 cgaatccctg aaggacatcc gggtacccct ctatgtcatc cctattgaag acatcaagag
    16621 aaatgccctc tgggtagccc acgtccgctc catggttccc ccgtttgata ttattttttc
    16681 aagcaatccg ctcgtaatgc ggttgttttc cgaagagggg atccccgtac gctctcctgc
    16741 catgtatgca agggaactac actcggggac agaaattcgg cggaggattc tcaagggaga
    16801 gcaatgggag aatctggttc ccgctcctgt ttcggagata atacgggaga tcggaggcgt
    16861 ggagcgtgta cgacagattg caaggaacga ttgtgatgtg accgagtccc ctgggggaca
    16921 ttcactgtga ggagggattg aactgttcaa acgtatcaga tcgatgttcg gtacgccaaa
    16981 ggagccagaa gatgtcgaat gcgcgttagg ggaactccct tctctccttg attcgaggga
    17041 gaaggagatc agccgtaccc tcatcgaaaa gacccagaaa caaaggaata caatccggga
    17101 tcttcgttca tccctgaaag aactcgtgaa ggatctgaca tcgaaagaga gagaggaagc
    17161 ataccacccc aaactcgaaa ccattgcaaa aaataccttg ccgctctttg aacgggcgat
    17221 gttctcctct ctctcaaaag atctccctga agatccagac cttttttatc ttgctgcaac
    17281 agagagcctc aaaggctgcg taaaatccct ttcagggcag ggaagatatt tgaggggcgt
    17341 ttttcccgag gagatgaaag agatcaggga gacggttgac cagatcggca gagaaatgaa
    17401 tgcgatgacc cccgccattg cagaagcccg gaagcagaga gaagtacttt cggcggttcg
    17461 tatcgatctc tcccgactca attcagctga gaaggagaag aaacatactg ctgatgaaat
    17521 aaatctttta agaggagaaa ctctccggga aacgggagat attgatcggc tttgcgatag
    17581 gatcagagcc gtcaatgaag cgattgatca ggggagtctt cgcaatcagc aggaggagct
    17641 tgccacagct aagcgtgaat tcctcgaaaa agaaatggca ttacaggctg atctcgcagt
    17701 agtagctcat gttttccgga aggcggagaa agtactgggc aggaccatag gaacagcttc
    17761 agtcaaggat attgaatctt ttgtggacac tctggccggg agcggcatcc ccgctgagga
    17821 gcagatagtg cccgatctca tcagattgtt acccactctt gcgagcatga gagattcagg
    17881 ggatctggtt ctgaaaaaca aggaagagaa ggagctcttt tcaagggaga tggatctgcc
    17941 ttcccggata cgggatgggt atgcacgaag agaagtggca tatcgtagtt tccatacaaa
    18001 ggagcatgcc tatcaggaaa atcctctcct ggtggaacta tctgccctgc agagagaagc
    18061 cgaccgacgg aaggggcatg ctgagtcctt aacgtcccgt ttatcatccc tcatggagaa
    18121 aaatgccgca ttggagaaag aaatccctga tctggaagaa cgggtacgtt ctgatgttgg
    18181 gagattactt gggcatgctg tgtcaattgc aatggatgat gctacggaac tatcaaggtg
    18241 atcttgtgat aactctcccg attggacagg tggtgtgaag gtgccagacg accggtatcg
    18301 tcttcttgag gtgacagatg gaaaggtccc ctgcctgcca gggaagaatg agacgatcga
    18361 acactgcagg ttttgtgttc attcaagatt tttccgggta agaggggaat atgtcaaatc
    18421 tcccgctctt gcctattgtc tcctccatcg cgccacgaaa gaggtggatc tgaaatctgt
    18481 tgacgcagta caatgcgctg atcgtgatgg agaaggatat cggagtatga tgaatattat
    18541 tggataatag ttacggggtt ttccagaact cccaccactt tttactctgg aagggcaccg
    18601 gtttcatctc ccgtaccagt tcgacatcga taatatggtg tatgagcttt acttcgtcgg
    18661 taagctccat gagggactgt tttaatgtct cgagggagag tttgatatcg tgatattctt
    18721 caaaggtctg tttctgattc gggctgaagg gagtggttcc ggttgccttg gtaaaaccgt
    18781 tctctatcat ctcaatgatg gcgcggttac ggtcaatgcc attctctttg gcatacttat
    18841 cgattagctc catcatctct gtatcagtag tgatcgatat cctgagcacc atgtctcctt
    18901 ctcccgtgtc ttttatttca tagaaattat cggtaaggta aaaaaaacca cgcctgccat
    18961 caatcgcaaa aaaaggctag aatgggatcc agccggacat gccgttcaaa ttcagcggcc
    19021 agatcttcgt atacatccct gtctttctct gttgcatcca tctcaaagtc cagatttttc
    19081 tttctgtaca gataggtgag cagtgcccgg actgcggctg gattctcgaa aaggccatgg
    19141 agataggttc ctattaccag cccgtcctct gaaactgccc cttctccttc gaatgcttca
    19201 ttgtcaccag tacgaattgt ctcgcccata tgaatctcgt atcctttcac tgatcccatc
    19261 tgggccagaa tcggcccgtg cggaagggct tcccgatgta cctgtttagt aaccttctca
    19321 tagcgggaaa accgtgtggt tacatcgagg aggccaatcc cctcatatct cccgcagcgg
    19381 gattcaagcc cttcatcaaa gatctctttt ccaagcatct ggtaaccccc gcagattccg
    19441 ataattggga tgccctgttg gcttgccctg acaatatcag cagcagtacc actcgcctga
    19501 agcagaagaa gatcttcgac ggtattcttt gtaccgggga taatgataca atcgtatcca
    19561 ccaagtggtt cacccggatg taagtaatct acggctgcat acggttcgag ccgctcaaag
    19621 tcagtaaaat tcgaaatacg ggggagtctg atgatcccaa tctttaccgg aaggttgcag
    19681 gatcgcttgt ctccaagcga cagggagtct tcgctcggga gagggattga aaataccggg
    19741 agaatcccga gcacggggat cccgcagagg tcttcaataa tctgaactcc ctcttcgaaa
    19801 agggccggat cccccctgaa tttattaatg atgattccca tcactcttgg ccggatatcc
    19861 tctgggagga gtgatagggt tccgtatatc tgtgcaaata ctcctccccg ttcgatgtcg
    19921 gcgacaagaa ggaccggcag gcggagttcc cgctctatcc tgatattcgc gatatcccgg
    19981 gaataaaggt ttatttcggc tgctcctccc gcaccttcgg caacgatatg gccaaatttc
    20041 tcgagaaggc gatgagcaga agcgacagcg atagagagga ggtaatcagt ctcctgataa
    20101 tattctgaaa tgcgaacgtc tttccagggc cgacccttga gcaccacttg ggagaccatc
    20161 tccccttttg gtttcagtaa gatcggattc atatcagcat ccggctctac ctgtgcagcg
    20221 agagcctgca ttgcctgtgc aataccaatt tcagcgccat cgctggttat aaaggaattc
    20281 aggctcatat tttgtgattt gaagggtgct acccttatgc cacggtttgt aaggatccgg
    20341 cagagggctg caacgaccgt gctctttccg acatgtgacg cggtaccgat gacaagaagg
    20401 gacatctgaa ggaaaggtgt gcatggagtt taaataaaag gtattccttg ttgttttcat
    20461 atggcgctgt ccgaggcagg aaagatactg cttgagagcc ggtatcttct cagcggagag
    20521 accgtgtcag atatgttcca acgcgtcgcc agggcagtga ataccggcag ggaagcgcag
    20581 ttctgccaga tcatggaaga tctcctcttt ctcccgaact ccccgaccct tatgaatgcc
    20641 gggaccccca aaggccagtt atcagcatgc ttcgtgctcc cggtagaaga ttcaattcag
    20701 ggcatcttta ccgccctcac acagatggcg atgatccaca aaagcggggg aggtaccgga
    20761 ttttctttct cacgaatcag gccagctggg gacattgttg gcacaaccgc aggggtcgca
    20821 agcggtcctc tttcattcat ccgtgttttt gatgaagcca ccactgcttt aaaacagggc
    20881 ggcaggagga gaggagcgaa tatgggagtc cttgcctctt cacatccaga tgttctctca
    20941 tttattaggg caaagaaaaa cggagacctg aaaaatttta atctttctgt cggcattgat
    21001 cgtcacttct ttgactcata ttgccggaaa gggacttatg atctcattaa tccaaggaat
    21061 ggagaagtct ctcgaaccat cgatacaggg atcctttggg aagaactttg ttccgcggca
    21121 tggcaatgcg gtgatccggg tatcctgttc cttgaccgga tcaatgattt gaacccggtc
    21181 cctggccttg gtccgattga agcaacgaat ccctgcggtg aacaacctct tctgccttat
    21241 gagagctgca accttgggag tctcaatata gcgggatttg ttcggaaggg tgagctggat
    21301 gaggacaagc tggtatcagt ggtcaggttc ggtgttgagt ttcttgacgc agtgattgat
    21361 atcaatacct acccggttcc cgagatcagg gatcggacgc ttcttaccag aaaaataggt
    21421 ctggggataa tgggtcttgc agatgccctt attatgatgg aaatacccta tgcctccaac
    21481 gaaggtctca atgttaccga atcaatcatg caagtgatcc agcaggaagc tcatttcgct
    21541 tctgaggaac taggtgaaaa aaagggatca ttccctgcaa ttgataagag ctggtatccc
    21601 ggtccgatga ggaacgcaac cgtgacaacg gttgcaccaa ccggatcact ccatatcatt
    21661 gcgggaacca gtagcgggat tgaacccctc ttttccctgg cgttttcccg tatcatcaat
    21721 ggaaaagcga tcaggtttgt ccatcccgct gtcaggcata ttgtcgaatc actgccaagg
    21781 ggtcagaccc tgatggaaca gatcgaaagg accgggagtg ttcagaatct gccaatccgg
    21841 gattatgaaa aggaattgct tcgtactgct cctgaaatca aaccggagtt ccatgtccgg
    21901 atgcaggcag ttgtgcagaa gaacgtggat aacgcagttt caaaaacagt caacctcccc
    21961 gaacatgcta cccttgagga gatatcagga atatttcttc ttgcaagaac tcttggatgc
    22021 aaagggatta ccgtataccg gtacaatagc cgggaagacc aggtgttatc acgaggatgc
    22081 gatacctgcc gggtagatgg tctgggcagc tgagggagca tggtatggat gatgcttccc
    22141 tacagcgctg tcaatccttt ttcattggag gcggtgacct gaaaggcagg tgtaatgcca
    22201 acaaataaat aaaagaggac ttgaacgtac ttgtaatgaa tggtgggatc tgtccgacgt
    22261 gtggattacc gaaggaactt tgtatctgtg aggaagttgc aaaagaacag cagcggatta
    22321 atgtcaaggt caacaagaga agatatggaa aggaagtaac cgtgatagaa ggccttgacc
    22381 caaccgacat cgaccttgaa gatctctcaa aatatttaaa aggcaagctt gcctgtggtg
    22441 gaaccgtgaa agagagttcc attgaattgc agggaaatca taaggaccgg gtcaaaaatc
    22501 tccttgccaa taagggatac agcgtggaaa atatttcata acaccatttt ttcgttctct
    22561 gggagttacc ctctcttccc aggtagccaa ccaattattt tcctgtttcc aaaccaaatg
    22621 gcattttaca attgctccat ggggatacca ttactatcgt ttccctgctg tataattgga
    22681 gatatattgg agatatgaat ccccggggaa agacgagtca ggtggagtgt cctggttaca
    22741 ggcctgaaaa aggaatcgtc aaagggtgaa aaatctgcgt tacctgagag aattacataa
    22801 aaaaacggac ttattaatca gatgctaaaa agatatgggt ataatactat ttttaccacc
    22861 aatcctgcga ttaaattaca tctaaaaata ttccattttt gcagttccaa aaatcgatga
    22921 aaaaaagtta aatcccatag agcaattcac aatgaaggaa taatgcggtt gataccatcc
    22981 tatcgcatct tgggggacgc cacagcggcg ggggtaagcc aagggcgcaa cccccaggag
    23041 cgtcaagagg tatgaaatca tgtgatggcg tgaaaaaatg gtggatgaaa tgccactata
    23101 ccaaagataa agaattgatt cccaatttca tcggtcggtc tttttcttct ttttcgcccc
    23161 tgacatcgag aggagatcaa ccgcatagct tccctcagct ccgagtccta ctacccgatc
    23221 atcacattgg gccatcttct cgatattgag ttcatatgtt ccgggtgcga cgattcgaat
    23281 gctctctaca cacgaacctg ttatttcctc aattttatgt ttttcaggta atggaggagg
    23341 ggttgaggtg ggagctttgg gggtaatgct cgattcatgt ttcttcttta agcgggcatt
    23401 tggacgttca aaataaaact ttttcccccc acactgtgag cagccgacca ggacctctgc
    23461 agaagagtca ggatagactt tcccgcattt ggcgcaggta tgaaccatat gttcaccggg
    23521 atgaaaccca ggcactgatc aaatccttgt ctttccggat catgcgcaac tggtttgctg
    23581 gcccgataac cgtcagtcga gattcgggcc gggttctgcc cagaagtctt tgcaggaaac
    23641 cgggcgcttc ctcatgagag gggtacgtct cgatctcaat gccgttaaaa ccatcgggaa
    23701 ggatctcatg catcgtcatc tcgatcagtg tgccctgctc ttcgggatca agtccttttt
    23761 caagaataac aatcgttccc tgtcggacat catcgagaat catgtgtatc ttttccatgg
    23821 aagtgagctt tgaaagccgt tcttctgaaa tcaggtcgat ctgaacaccc tgttgcatga
    23881 tcattcaccc aaaatgtaat accatctttt catagagctc ctcgatattg ctcccttctg
    23941 tgctggagat agggatgaca ggatgctggg gaaaagcagt cctgattcgc tgcgggcttg
    24001 catcagggag atcaattttg tttgcaacga taaggaccgg gagttttctg ctctcgatga
    24061 ttcctaccat catgatattc acctggagaa agggatcctc ggtactgtca agcatgtata
    24121 tcaccccatc gatatcttca cgaagccagt gcatagcctc ggcaacccct tctgtagcct
    24181 cacgggcacg agtgattgcc tcttccttgc ccactccata ctccaggaac tctttataat
    24241 ctatcttcgt ggtcactcca ggggtatcca caatatctat ggttatggtg ctcccgttgt
    24301 agccggtaat ggtaatatcc tgcttccggg ttgctctcct ggtttcgtgg ggaatctcac
    24361 tcacgggacc gaccacctcc cctgtccagt cccttgcaat gcggtttgca agtgttgtct
    24421 tacctgcatt tgggggaccg tagattccaa tccgagagcg ctttttttta aaaaatgcca
    24481 tgagaaattt cgagacgcga tttttagcct ggacaaaaat actcatacct cacctcatcg
    24541 tggcaattga accggatacc cttgcaatgg ccgttctctc tcatataccc ttttgaagaa
    24601 tgtcattatt attgttttgt actggttcaa ttgcctgttt tctactggtt cagttgcctc
    24661 tatgccaaag gagatttaat acgcatcgat cagttaataa tgatggctcg cagtaatgga
    24721 tactgcgtat cccgttcata agggacgcca attgggggtg gatgattgaa aaaaacctca
    24781 gactcgatcc ggttcgaaac gcgggtgccg cttagtgaag tgatgaaggg taatcccacc
    24841 accattcagt ccgatgcatc cgtcgctcgg gcagccatgg ctatgtgccg tgatgaggtg
    24901 gggagctgta ttgtgctccg tgacgacctc ccaatcggga ttgtgaccga agaagatatc
    24961 aattgtaagg tggttgccaa ggacaagagg ccaagtgcgg tgttggttaa cgaagtgatg
    25021 agtacacccc tcatcaccat acgttcagac aagaccgtac gcgacgcggc tcacatgatg
    25081 atccggaatc gtgtccgcag actacctgtg gttgatgatg aaaacagggt catcggtatc
    25141 gttaccgtca gggatattct gaccgtctca actgagatca acgagctcat gaatgatctt
    25201 atcgagataa acaggcttga agagattgaa gtaggtacct gcagccgatg cgggcagatg
    25261 tccgatgatc tccgccgtat cgataatatc atgctctgca catcatgccg tgaggaggaa
    25321 cttcttcaat gaagacagcg gcagatttca tggttgagtt tccagaactc aagtcggatg
    25381 accgtatcac gaaagcacgc aagatccttc gtgaaaacac cttccgcgag gtctatgctc
    25441 atgatgggaa acgaaagctt gcaggataca tcgatatcac cgatgtcctc catgttactg
    25501 ccacgaaatc tgacgtcaca atcgaagggt atctgaagga tatcacccct gtttctcagg
    25561 gggatacgct cggaatcgtc cttgaaacca tcaggaagaa taaaacagat agtgtaccgg
    25621 tcgtagatga tcacggtatc ttcctgggag gggtcctttt atctgaactc tttccggtcg
    25681 ttctgaccat gcagcggctg catggacctg tgtgcgattc catgacaagg aatgtcatct
    25741 cctgttcggc agaggatacc gtccagcgaa ttcatgcact tatcatcgat accgggtact
    25801 ccgcctttcc ggtgatcaag aagaagaagg ttatcgggat aatttcgcgg cgcgatctcc
    25861 tcaaggacgg gagatggcgt acctcatcag agacaacaac caccgttgat accctcatga
    25921 caaccccggt aatcactgcc gggcctgatg atgacatcag ggccgctgct gattgtatgg
    25981 taaaacatga cataagccgg ctgccggtca ttgagaatga tgtgatcgtc gggataattg
    26041 atcgccatga tgtcctgaac agcatggccc ggtaagggat ggaactacag gattatccat
    26101 tcgccacgga ccggatgatc gcctctctcc aaccatattc atgaaagagg gatactatgc
    26161 aaacgaatga ccgtaatttt aaacagggcg acaaactcct gaaaatgccc ggtaaactcg
    26221 atcggggacc ggtcgagttc aagtcccaca tcgtgacccg ggaaggtgag atcatggcca
    26281 tcgcaacacg ggatgtcgtt tcggttccgc cgacaatgcc gattctcgca gcggttgaga
    26341 cgatgaccaa ctgcggcttt cgacggcttc ccgttgtgga tgcaggaagc agaaaattga
    26401 gggggattgt aacatcaggg gatatcatca atttcatggg gggaggcgat aaattcaacc
    26461 tggtccggat caaacatgat gggaatcttc tgtctgccat caatgagccg gttcgcagca
    26521 tcatgaccca gcagatgaca acccttcccg attctgccgc tatcaatgat gcaattgata
    26581 tcatcgtcaa ccggaaagtc ggcgggatgc ccatcgttga caatgacggt gtcctggttg
    26641 gtatcgtgac agagagggat gtcatgaagg tccttgcaac agaacgatcg ggtctctgtg
    26701 tgaatgacat catgagtact gcattgagaa ttaccaatcc tgactgccct atccggattg
    26761 tcacacggga catgacgacc aaccgtttca ggaggctccc tgtggtgagt aatgatgtcg
    26821 tatatggcat tatcacaacg acggacatca tgaaatacct cgggacgaaa aaggtgttct
    26881 ctcaacttaa aacgggtgat atcgatgaag tgatggatct cgcggtgcga aacctcgtat
    26941 cagggaacct cttcacgata tccccagaaa ggagcattaa tgaagccgca agggagatgg
    27001 tcgatcggaa tgttggggcc cttccggtga ttgagaatac acggctggtc ggtcttgtca
    27061 ccgaatttga cctggtcaaa gccctttcaa ggaagtgagc ctggaatgaa tgtaggcgat
    27121 gtgatgagct ccccggtgtt tgtcatcgat tcaggtgaaa atgttgccag ggcgcgaaat
    27181 ttgatgctta aacacaggat ttcacgcctg ccggtccttg agaaatccct ccttgccggg
    27241 attatcacaa agaaggatat cgggtaccgc ctccgccaga gcgaaccttt gtggcgaagg
    27301 aggccaatcg aca
//
LOCUS       DAOOCO010000005        26415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_1521_length_26415_cov_4.850759, whole genome shotgun sequence.
ACCESSION   DAOOCO010000005 DAOOCO010000000
VERSION     DAOOCO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 26415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26415
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_26415_cov_4.850759"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..857
                     /locus_tag="PK154_00650"
     CDS             234..857
                     /locus_tag="PK154_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPW09602.1"
                     /translation="MRPLMIDLTGKKVVIIGGGEVGARKARFFAPAAEVTVISRSFSP
                     HLSGIDVKRIPCDIRDLPDGKIRALLDGAFAVVAATPDPSLNNRIGTLCREKGILFNN
                     ASGDTGDLLIPSVIAGDHFLLALSTEGESPAVSRFLREHLQSTLPELDRMVTLQGRLR
                     KYLLDIEPDSRKRKDIVIKVLHDPSVWVALAKSEEGAWQIIEEKYLQ"
     gene            854..2128
                     /gene="hemA"
                     /locus_tag="PK154_00655"
     CDS             854..2128
                     /gene="hemA"
                     /locus_tag="PK154_00655"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPW09603.1"
                     /translation="MNQALFSPIAIAGLSHHTATVDMLEAFRFPDEEAFLRKAKDRFR
                     GILLLQTCNRIEILVHGEASSLVSLLQEEGREDFSVLSGIDALRHLLELAAGMDSMII
                     GEDQIIGQLKTALDFARERGCASPVLELCVNKAVHAGIEVRKQTQINRGAVSIGSAAV
                     LLAEEQLGSLEGKRILVVGSGEMGMLVAQALAAKQLTAIYVANRTYGRAEVLAKKIGG
                     KAVRLDDLPRYMLLSDVVISCTSAPHPVIHCDDLKEIMRGRCWPLEGHPRPLILVDIA
                     QPRDVEEGAEAIDGVHLFTIDSLRQINENTMNSRKSEAAKARAYLDDELAHFIRLLNG
                     KAADDTLAILHTWAESIRTRERDRAISRIGTGDERTREIVDDLTRVLANKILSDVTFS
                     IRLCAEEGDLDYAESLVAAITRGERLCFRNKE"
     gene            2107..3090
                     /gene="hemB"
                     /locus_tag="PK154_00660"
     CDS             2107..3090
                     /gene="hemB"
                     /locus_tag="PK154_00660"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPW09604.1"
                     /translation="MFPQQRMRRLRGRLIQPFVKEHHLRKTDLVMPLFIDEIAERPTP
                     IPTMPGQFRYPLAGIGPVVRQLQERGIGAVLLFGIPIRKDPEGTEAYNPRGVIQLAVQ
                     RVKSEAPGVLVITDVCACEYTSHGHCGIIGEGPTGMDLRNDPSLELIAKIAVSHARAG
                     ADLVAPSCMLDGMVSAIRTALDDEGFQETGIMSYSTKFASGLYGPFREAADSGCGFGD
                     RSTYQIHPANSREALRESEIDAREGADILMVKPGGFYLDIVSSVRALGLPVAVYQVSG
                     EYAMIRAAAMNGWIDEKTVVMESLICAKRAGADLIITYYAADAAGWLDEEQ"
     gene            3077..4315
                     /locus_tag="PK154_00665"
     CDS             3077..4315
                     /locus_tag="PK154_00665"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPW09605.1"
                     /translation="MKSSDYFEQARMLMPGGVSSPVRAIKPHPFYTKSAQGSRIATVD
                     GEELIDCCMAYGPLILGHAHPLIKEAIATQLGKGWLYGTPCPLEPEYAGMVIRDHPGM
                     EMLRFVSSGSEATMAAIRVARGFSGRPDIVKVEGGFHGAHDGVLVKAGSGATTLGIPD
                     SAGVLADVVAHTWQVPYNDPDALERLLFRHPEIGAFIIEPVPGNIGPVLPEKGYLQEV
                     RRITRAHDVLLIFDEVITGYRIGIGGAQEYYNVRPDLTTLGKIIGGGLPIGAFGGRRD
                     IMERIAPAGPVYQAGTFSGNPLSLAAGMATIQWLHAHRGIYRELDSAGKRIGEAVTGK
                     GGSFVQHGSMFKFFFRTAPPRNYREVKECDTGRFSSFWSAMLKKGIFLPPSQFETNFL
                     SAVHNEVDVERISDAYRSCL"
     gene            4306..5196
                     /gene="hemC"
                     /locus_tag="PK154_00670"
     CDS             4306..5196
                     /gene="hemC"
                     /locus_tag="PK154_00670"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPW09606.1"
                     /translation="MPVRIGTRGSRLALAQAERVASLLQGCGTAPEIVVITTKGDTVD
                     GVPLHEIGGQGVFVRALDDAILDGRIDAAVHSMKDIPAKRPAGLCTCAILKRDSPADF
                     LVHTIPRKEIRVIGTTSTRRRAQLIRYMEGVSVKQLRGNVDTRLRKLAEEQYDAIVLA
                     EAGLERLGLSVSGTRLSPQRFVPSPNQGTIAVVCRDEAPLCEVFAPLDHEQTRIDVQM
                     ERTVMEEVGGGCFTPQGIYAHHGHLIGEILSLDGTRRIRREDDVGSLEDARTFGQYLR
                     KEGEELIREAYEKLRICDEG"
     gene            5186..5935
                     /gene="cobA"
                     /locus_tag="PK154_00675"
     CDS             5186..5935
                     /gene="cobA"
                     /locus_tag="PK154_00675"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPW09607.1"
                     /translation="MKGKVYLVGSGPGGAGLLSLRAREVIDSAEVILYDQLPGEEILL
                     SLPEHAEKIDCGKYGSDHTLEQEEIEELMVRKAREGKRVVRLKGGDPFLFGRGGEELE
                     VIREEGIEVELVPGITSALAVPGAVGIPLTHRRYASSVTILTGHEDPTKEESALDWAL
                     LAKQSGTIVILMGVKNLPLIAGLLIREGKDPDTPVAIIERGLRKDQRVTTGPLARIGA
                     LAKERHVKPPAVIVMGGVVNLYREGDGIIPL"
     gene            6004..6531
                     /gene="cbiT"
                     /locus_tag="PK154_00680"
     CDS             6004..6531
                     /gene="cbiT"
                     /locus_tag="PK154_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HPW09608.1"
                     /translation="MSLPGGPTKDEIMAISLAKLSLEPGDIVADIGCGTGTVSLEAAR
                     RVAKVYAVDLREEAVSCTRDRAMAAGMTNIEVSLGHGMDLIKAVPHIDAAFVGGSRGL
                     AGMLSLLSEKKVRSVVVNAVLLSTLHEAVTTMQELSMFKEAIHVSVARSAPLGGGLMF
                     RPLDPVYIVVGGAPC"
     gene            6525..7133
                     /locus_tag="PK154_00685"
     CDS             6525..7133
                     /locus_tag="PK154_00685"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HPW09609.1"
                     /translation="MLIGLGLGPGEPEYLTLRAVRILREADAVFVPGMIARELVAPYR
                     DAEILDFPMTGDEERIRTCMEQNADRIAGIARTGTAVLGILGDPNFFSTYSRLTAILN
                     VCHPGIRCRTEPGISAITAFASVAGISLSGGFLVTDGPVEPRSRIMLKVRRPRQVAES
                     LKREGFRSFVLVERMCMEGMQIYRDDQLPEESDYMSVLYAER"
     gene            7134..7850
                     /locus_tag="PK154_00690"
     CDS             7134..7850
                     /locus_tag="PK154_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HPW09610.1"
                     /translation="MPHIYFVGAGPGDPGLITVRGMDLLSRAEVLVFAGSLVNPELVE
                     RSSARIKLDSWGKSLEDIVPLMIREAQNGALVVRLHSGDPALFGAIIEQMQLLEEAGI
                     TCEVIPGVSSLFAASAALGTQLTIGGISDTLIVTRPAGKTLAHDEIHELSAHTATMVF
                     FLGADRLDEITGKLQYPASTPAAVVYHASWPDQKIVRGTVADIAGKAAEAGITRSALL
                     VVGGVLDPSGSGHRRSILYS"
     gene            7847..8716
                     /gene="cbiG"
                     /locus_tag="PK154_00695"
     CDS             7847..8716
                     /gene="cbiG"
                     /locus_tag="PK154_00695"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HPW09611.1"
                     /translation="MNRAVIYLPRFSEAAGRIARVLGADLLEYSETAFSTAISRYREI
                     AAVMSMGIAVRGIAPYLKDKWTDPALVVVTPDLRLAIPVTGGHHGANALARELAVLGA
                     VPVITTATEREGVPSVEGIAAAGRYSLVNRDASKPVNAGFLDGTAGFYSVKGPAVVIG
                     DKDTAFLITDGEYSIGIGCRKDTSAKEIEEAVRTVLATSGIRPDQVFIYATTAKKTHE
                     SGLLDAVRALGRPLLYLDDLTINAIPTRSGSRASRIGLSGVAEPCALATAKRRELVVG
                     KTVIGGVTIAIAR"
     gene            8700..9482
                     /gene="cobJ"
                     /locus_tag="PK154_00700"
     CDS             8700..9482
                     /gene="cobJ"
                     /locus_tag="PK154_00700"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HPW09612.1"
                     /translation="MPLHGNRKGMLFIIGIGPGSPDQMTGQAREALLQSEVIIGNDTY
                     LSQIGALISGKEIIRSSMGQEVERAKRSIELSRSQIVAMVSGGDAGIYGMASIVLEVL
                     EHEPCSVPVRVIPGITAANAAASLLGSPLSGDFAAVSLSDLLTPLEVIERRLHAAFSM
                     GTPVVLYNPKSRGRPHHFALAMEIAGKYREAATPVGIVKNAYREGEEVMVTTLGEIGH
                     AEAAVDMHTTVIVGGEESRIWRNGDDVKGIITPRGYHKKYVY"
     gene            9430..10098
                     /locus_tag="PK154_00705"
     CDS             9430..10098
                     /locus_tag="PK154_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HPW09613.1"
                     /translation="MSKESSPRGVTTKNTYTDLGATTAEAYEISRTSRALAQKEIGDA
                     DYEDRVRQRCSIAVGDFTMAGLLRFTKNPVEAGLAALSRAAPLVTDIRMVQVGIQKKG
                     HSSPVLCALDYGADLAESREITRSSAGILALKDHLAGSVIVVGNAPSALLAICDLVRE
                     GCRPALVIGTPVGFVKAAESKEELRSLDIPSISNEGTRGGTPVAVAAINEIITIHAEL
                     KRQP"
     gene            10073..11107
                     /locus_tag="PK154_00710"
     CDS             10073..11107
                     /locus_tag="PK154_00710"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HPW09614.1"
                     /translation="MQSSSASPDRITDPVTGFTYPGPWIAACSDEEGLDLVRAGLAVL
                     TSSGMILRRGYTTGTTAAACCKAATLSLAEVPGKVSVLTPSGIRVCVPVEATCGIASC
                     FKYPGDYPGDATAGIQFIAECNPADNGISLYPGEGIGRFTRDTPRNRKGDPAISFPAL
                     SSILAAIQEGLETTGLPGASIHLRVPRGEIVAKDTLNPKLGIEGGISILGTTGLVEPW
                     DDHLTKTVMERVRTADRVVLTTGRTGLRYARLLFPGYEAVLAGSHLREALGEAKGQVI
                     LCGLPALILKFLDPEILSMTGFDTVEEFTTRPEFRARMQASFLKGKEQYPRLRVVIVD
                     RNGTIMGDSG"
     gene            11104..11715
                     /locus_tag="PK154_00715"
     CDS             11104..11715
                     /locus_tag="PK154_00715"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HPW09615.1"
                     /translation="MKVVGVGCGPGMLTEQAASAISDAAIIFGSKRAIALVAAHIPAG
                     CIVREMVTFSPNQSIPDNAVILSTGDPQLAGLGYLGGEIIPGISSLQVAAARLHVPLE
                     QVSIVIAHGKDHGRALNRIADEVKRGQNTFIIADPKFSITALCTALEGAGEDISIAVC
                     EDLGYPDEKIRIGSIASPPQPVSPLFSVMVGRIESPPGDQKSH"
     gene            11768..11947
                     /locus_tag="PK154_00720"
     CDS             11768..11947
                     /locus_tag="PK154_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09616.1"
                     /translation="MEKATKKQLSLFCFLAGFILVLIQDLKGFSTIDMGFSWPWAIMF
                     YTGLLLMMIGYYLRG"
     gene            complement(12015..12845)
                     /locus_tag="PK154_00725"
     CDS             complement(12015..12845)
                     /locus_tag="PK154_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPW09617.1"
                     /translation="MRERPEPTRQYAGEFRDRIYHFYRANRRDLPWRETREPYRILVS
                     EFMLQQTQVARVLEKYDGFITRFPDFAVLHAAPVEDLLREWQGLGYNRRALALKRTAS
                     LIMNGWSGVLPDDEEALLSFPGIGKATAAAIRAFAFGKPSLLLETNTRRVFIHCFFHD
                     RPVVTDKEILPLLEETLDRENPRDWYYALMDYGAYLGRTNENPNHRSQQYKKQSPFEG
                     SVRQVRGKILALLVKHGTLTREELCRETGLPQDQIDPVLAVLAKEGFIALDRDMVKIT
                     "
     gene            complement(12829..14211)
                     /locus_tag="PK154_00730"
     CDS             complement(12829..14211)
                     /locus_tag="PK154_00730"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPW09618.1"
                     /translation="MIPEERVAYLSKKSVKKGHYILCWMQAAPRITCNHAYQYAVRMA
                     ERHSLPLLACFDLIPGYPGATCPHYRFMVEGLIALSRDLEAQGVRFIVKTGPPGTSAR
                     ELGGDAALVVTDRGYLRFQDQWQQQVAGELDCLLVRVETNVVVPADIASEKEEWSAAT
                     FRRKITPHIDRFLLPLEEKKPSRSSLGLERGFEREMTVESLLSGIPGIIPEQGGTLQP
                     YPLPWKGGEEAAAALLKRFIARHLDHYPVERNDPSAGALSCMSPYLHFGQISPVFIAQ
                     NILRSGSLSAAAYLEELIVRRELAINFVHYNPLYDDYAGLPAWAKKTLSVHSEDRREY
                     SYGFSTFESARTHDPYWNSAQQEMVITGKMHGYMRMYWGKKILEWSETPEEAYQTALT
                     LNNRYELDGRDPNGYAGVAWCFGKHDRPWKERPVFGTIRYMNAAGLNRKFDAGRYAAT
                     INGLRDERKT"
     gene            complement(14290..14625)
                     /locus_tag="PK154_00735"
     CDS             complement(14290..14625)
                     /locus_tag="PK154_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09619.1"
                     /translation="MKEGIKATIILMLLAIFIAGSVAADLPWSGPISTSTSVSVEGTV
                     NETVSFTKTTIGSREKTVFTDTFTTVNPSGLSWRSHITLHGDPEKVWVDYTRTGAVPP
                     ISWRQLFSR"
     gene            complement(14726..16507)
                     /gene="mutL"
                     /locus_tag="PK154_00740"
     CDS             complement(14726..16507)
                     /gene="mutL"
                     /locus_tag="PK154_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW09620.1"
                     /translation="MTPGQGTRIQVLDESTVSRISAGEVVERAASVVKELCENSIDAG
                     AGRIRIAVTSEKGRITSIQVSDDGCGMSPDDAQLAFARYATSKIACIDDLFACSTLGF
                     RGEALASIAAVAKVTIVTKSREGAGISGTRIVNQGGEILLVSETGCPFGTVITVEQLY
                     FNTPAREKFQKTVSSEIARITGFIERLVITHPGIAFELTHNDRKILSVPGGGRLMDTI
                     IHLFGLPVSRQMIRVHAESEGIFVDGYISVPELTRKNQYQMFISVNRRSISSRILSNA
                     VREAYGTLLPTGRYPVVFLDLSIDPSEVDVNVHPAKREVRLSREEEVRKVLISAISES
                     LSGASLIPEAGSSGDSVSGAEHPGRIRYRLPDLGGETGVKEPLLRDRISTERQLSLTG
                     RQPDTSVESKLPDFQIIGQLGSLYILAGTPGDDLLVIDQHAAHERVLYEQVTEYAEQH
                     HDLQELLEPVFLSLSPGESEALSAALPLLAEEGFCIDAFGPGTFLVRTVPVLFGQNIE
                     PGEIRDIIAGIIGPGMPRGIDTREQVRRIVACRGAIKAGAFCTLDQCRELLQQLARTR
                     NPFTCPHGRPTMIVFRKQKMDDLFKRV"
     gene            complement(16511..19159)
                     /gene="mutS"
                     /locus_tag="PK154_00745"
     CDS             complement(16511..19159)
                     /gene="mutS"
                     /locus_tag="PK154_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW09621.1"
                     /translation="MANGEEHPGPNHGRLTPAMAQYWAIKEQYRDAILLFHIGDFYET
                     FGGDAETVSRELEITLTSRSKDSGGQKIPLAGVPCHSVEGYIARLIAKGYKVAICDQV
                     EDAKNAKGIVRREVVRIITPGTVIDEGMIGSSGARYLMAVASLERNSVMGLAFLDITT
                     GEFVVTTCPSDRGTAALMSELTRYRPSECLMPEYLSAELGDIVKQSGVVVTAAGNCAT
                     DPASARKILIDHFKVSDLSDFRIESAPSVVMAAAMVLCYAKETQRLDLTHVQQIFYRQ
                     AGEACLIDGITLRNLEILESIRGRSGDPTLLSLLDRSMTPMGSRLIRSMLAVPLVSPA
                     AINRRLDTVEYFSRKTAVRARVREILGELADISRIAGRIACTNAGPRDLIALARTLGQ
                     LSAVRETLDDPSLPVELAGSCGDLVDEGWIAPLILNAIADDPPALARNGGVIREGYHP
                     LLDELRSRAASGREWIITLQQQEREKTGIKSLKVGYNSIFGYYIEVTKPNLALVPPTY
                     QRKQTTASGERFTIPELKEQEALIATAEDRLSSLEQELYMDVLRKLNDGVPSLQSVAR
                     GIAAIDAASALAEAAVLQQYTRPVIDETQDILIREGRHPVVEPGMKGGFVPNDTVLSS
                     SDSQILIITGANMAGKSTYMRSVALTVVMAQAGSFVPAAHARVGIVDRIFTRVGAFDD
                     LASGQSTFMVEMIELANILNNVTPRSLVILDEIGRGTSTLDGYCIARAVLDFLHGRGA
                     SGPKTLFATHFHEIAAAEHDLKRVRTYHFAVKDTGKDVIFLRKLIPGATDRSYGIHVA
                     SLAGLPGRVITRASALMEDLIRGEGGGGPRLQRYTQMLLVDAPPLVEHPVVSDLKKID
                     PDQLTPRDALGILYELKKRAGERKRD"
     gene            19370..21517
                     /locus_tag="PK154_00750"
     CDS             19370..21517
                     /locus_tag="PK154_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPW09622.1"
                     /translation="MMEPELRGDTNDRQGRKRPNRLINEVSPYLLQHAWNPVEWYAWG
                     DEAFAHAAAEDKPVFLSIGYATCHWCHVMERESFEDPDVAGVLNTHFVSVKVDREERP
                     DIDALYMAAGQMLSGMGGWPLNVFLTPEKQPFFAMTYMPRERRFGTPGIIDILEEISR
                     LWSEARPELIRTAGLTAAHLSRETGKGRRLQRTILDAAYHELLLRFDVRDGGFGGAPK
                     FPLPHTLLFLLRYSQLKGEERAISMVEKTLRSMAMGGLYDQIGSGFHRYSTDTHWLVP
                     HFEKMLYDQALHVLAYTEAYQLTSNEWYGTIARESLEYVLREMTAPQGGFFSAQDADS
                     GGEEGGYYLWTKKEVEDLLDRETAAAACRAWHVTTAGNYIDPVSGERTGRNILHITRE
                     ITDLAQGMGFSPEKLTSLIETAKKTLFTARKNRAPPLTDDKILADWNGLMIAALAKGA
                     RIFHEERYRNAAARAAEFILGSMKNPDGQLLHRYRNERAGITAQAADYSFLIFGLTEL
                     YMATFDPAHLAAALSLQESLDSQFWDPANGGYFTAAGTETDLIARQKEFYDGAIPSSN
                     SVAFTNLLRLTMLTGNTSFESRASDLSRPYAEHLAQAPAAYPFFLSGLCLLFGPSSEI
                     VIAGGEKGDTAEEMVDALECRYLPFTVILVKDSRNEAALAKAAPFTRDMGAIDGETAA
                     YLCSRHSCSVPVKGVKELLLGIEKEKDRKKSWV"
     gene            complement(21526..22002)
                     /locus_tag="PK154_00755"
     CDS             complement(21526..22002)
                     /locus_tag="PK154_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW09623.1"
                     /translation="MDIVKIPRMEKQDYDHLIEEGYVCRIAFQGEKYPYIAPFLYVFD
                     GKFLHFLSTKYGKKIEYFRKSPHVAVEVEKYTRDLSCYTFITLQGYLEEVTDSIDKKI
                     IREDFVNLIKGRKLSNNILAALGHSPQDPPEAIALEERSLVWRLTGVKDLVALRNL"
     gene            complement(22406..23776)
                     /locus_tag="PK154_00760"
     CDS             complement(22406..23776)
                     /locus_tag="PK154_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW09624.1"
                     /translation="MIIRKIPSEGLSHYSYFISAAGMAAVVDPRRDCEAYLELARELD
                     ARITHIFETHRNEDYVIGSFELASRTGAAIYHGSQISFAYGNAVKEGDTFRLGPIELR
                     IIETPGHTFESISIIVTDREVSDLPYAVFTGDALFAGDTGRTDFFGPERRAEVSALLF
                     ESIWKKILPLGDGTIVLPAHGAGSVCGSAISDHEVTTIGYEKQTHPHLSLSQGEFIQQ
                     KVNEHHYLPPYFAKMEQMNQQGAPVLSHLPDLRPYTVDELRDIQEKSQVLDIRSPTSF
                     AGGHIGGSLSIWKDGIPGFAGWFLSYDHPIILVDDFNCHLREVSRHLLRLGYDNLEGY
                     LAGGFPAYFKSGSPIGQFEAWSVHDLARERKKGDLFLLDVRDIANVRKYGMIPEAHHI
                     YVGELPGKVGEVPMDRKVAVYCDAGFKGSLGASYLAGKGYLRLANVLGGMGAYLRAGY
                     PVEHVQ"
     gene            23883..24590
                     /locus_tag="PK154_00765"
     CDS             23883..24590
                     /locus_tag="PK154_00765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW09625.1"
                     /translation="MTGLFKTLLFDMDNTLFDLVNAKIEACKAVTARLDAGDAEDLFS
                     YFLRRQGGFDDPENIRDFMLDHSCYNRGLFWECISLYREIQTRNLTPYPGVEQTLALL
                     ASSGFRLGIVTDAWLADATTRLEATGLSPFFDHVVTHEMTGAHKPSSLPFLLALVRLN
                     ATARETLLVGDSPRRDIAPAGILGMYTVYARYGDRFSHPKSDGGADHAIDSFPELLPI
                     LEPGGRNAKAARQATLS"
     gene            24655..25071
                     /locus_tag="PK154_00770"
     CDS             24655..25071
                     /locus_tag="PK154_00770"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPW09626.1"
                     /translation="MTRKRVLFICSHNAARSQMAEGYLRARHGDRFEAFSAGTKASTL
                     SPYAIRVMQEIGVDISGHRSKSLNEIAGREMDVAVIVCDAAKSACPFFPWAKVTLHAI
                     FRNPKEFAGTDDEIINGFREIRDEITGWIDRFVSEK"
     gene            complement(25361..25834)
                     /locus_tag="PK154_00775"
     CDS             complement(25361..25834)
                     /locus_tag="PK154_00775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW09627.1"
                     /translation="MTDQKSDPMMGSPAPEFCLPDAEGEEYCLTDFAGKWLVLYFYPR
                     DNTPGCTLEARGFSEAIEEFARLGAEIVGVSADSEASHQKFTAKLGLRFRILSDQKHA
                     AIAAYKVWKDHVLLKGIERSTFLISPGGTIAAVWRKVRVPGHVEEVKETLQKVRG"
     gene            complement(26071..>26415)
                     /locus_tag="PK154_00780"
     CDS             complement(26071..>26415)
                     /locus_tag="PK154_00780"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPW09628.1"
                     /translation="LLGMTVEERLSIGTVVVRVISNEDISGLLLFLRAHSFGVTIADG
                     EGSKGKVKIILSVIKRQDLEEVVRGIQHHLPGTFYSVEEIKSVAEGVFPERKSRLLFN
                     YRDPFGFFRKGK"
BASE COUNT         5832 a         7350 c         7509 g         5724 t
ORIGIN      
        1 taggcgttgg caacgcctag tgataaccac tacactatcg gcgcaaaatg actctgcatt
       61 ataggcattg aatcattata aggatatcga ataatcgcct gaatggtatc cctggacaag
      121 ttcaagtggc tgggagaggc cggttcgttc tggccgagtc atcggaacag aagatcccgg
      181 gattcgcctc gattccaaca tcttaatttc acgtcacgcc agatcatatg cacatgcgcc
      241 cgcttatgat cgaccttacc gggaaaaagg tggtgatcat tggcgggggt gaagtaggag
      301 cacggaaagc acggttcttt gctcctgcag cagaagtgac cgtcatctcc cgttcatttt
      361 ccccacatct ctccgggatt gatgtgaaac ggatcccctg cgatatcagg gacctgcctg
      421 acgggaagat acgcgctctg ttggacgggg catttgcagt cgtcgcggcg accccggatc
      481 catcgctcaa caaccgcatc gggaccttgt gccgggagaa gggtatcctg ttcaacaatg
      541 cttcgggtga tacaggcgat ctcctgatcc cctcggtgat tgccggtgat cacttcctcc
      601 ttgccctgag cacggaggga gagagccctg cggtgtcccg gttcctccgt gaacacctcc
      661 aatccaccct tccggagctt gacaggatgg tgacattgca gggaaggctc aggaagtacc
      721 tcctggatat agagccggat tcgcggaaga ggaaagatat cgtgataaaa gtcctccatg
      781 atccttctgt atgggttgca ttggcgaaaa gtgaagaggg tgcatggcaa atcattgagg
      841 agaagtacct gcaatgaacc aagccctctt ctccccgata gcaatcgccg gcctcagcca
      901 ccacacggca acggtagaca tgctcgaggc cttcaggttc cctgacgaag aggcgtttct
      961 gaggaaagca aaagaccggt tccgcggaat cctgctcctc cagacctgca accggatcga
     1021 gatcctggtt cacggcgagg cttcaagcct tgtctcactc ctgcaggaag agggcaggga
     1081 ggacttttct gtcctttccg ggatagatgc actccgccac cttctcgaac ttgccgctgg
     1141 catggattcc atgatcatag gtgaggacca gatcatcggc caactcaaga ccgctctcga
     1201 ttttgcacgg gagagggggt gtgcaagccc ggtccttgaa ctctgcgtga acaaggcagt
     1261 ccacgccggg attgaggtgc ggaaacagac ccagatcaac cggggagcgg tatccatcgg
     1321 atctgccgca gtcctccttg ccgaggagca gctcggctcc ctggaaggaa agaggatcct
     1381 cgtcgtgggt tctggggaga tggggatgct cgttgcccag gcgcttgcag caaagcagct
     1441 caccgcgatc tatgttgcca acagaaccta cgggcgtgct gaggtgcttg ccaaaaagat
     1501 cgggggaaaa gcggtgaggc tggatgatct cccccgctat atgctcctct ctgatgttgt
     1561 catctcgtgc acctcggccc cccatcccgt catccactgt gacgacctga aggagatcat
     1621 gagagggcgg tgctggccgc ttgaaggcca tcccagaccc ctcatccttg tggatatcgc
     1681 ccagccccgt gatgtcgagg agggcgctga agccattgac ggagtccatc tcttcaccat
     1741 cgacagcctc aggcagatca acgagaacac gatgaacagc cgcaaatcgg aggctgccaa
     1801 agcccgggct tacctggatg atgaactcgc tcattttatc cgcctcctca acggcaaagc
     1861 agccgatgac acactcgcca tcctccatac ctgggctgaa tcgatccgca cccgggagag
     1921 ggaccgggcg atcagccgca tcggaaccgg ggatgagaga acgagagaga tcgttgatga
     1981 cctgaccagg gttctggcaa acaagattct ctccgatgtc accttctcga tacggctctg
     2041 cgcagaggaa ggagatctgg actatgcaga gtcgcttgtc gctgcgatca ccaggggtga
     2101 acggttatgt ttccgcaaca aagaatgaga cggctccgcg ggaggcttat ccagcctttt
     2161 gtgaaagagc accacctcag aaaaaccgat ctcgtgatgc cgcttttcat cgacgagatt
     2221 gcagaacgtc ccacgccgat ccccactatg cccggtcagt tcaggtatcc gctggcaggt
     2281 attggcccgg tggtgcgcca gctccaggag agagggatcg gcgccgtgct cctcttcggg
     2341 ataccaatcc ggaaggatcc tgaaggaacc gaagcataca atccccgcgg ggtgatccag
     2401 ctggccgtgc aacgggtaaa atccgaagcc ccgggagtcc tcgtcatcac cgatgtctgt
     2461 gcatgtgaat acaccagcca tggtcattgc gggatcatcg gagagggacc caccgggatg
     2521 gatctccgga acgatccctc ccttgaactg atagcaaaga tcgcggtaag ccatgcaagg
     2581 gcaggcgcgg atctggttgc cccttcctgc atgcttgacg gcatggtttc ggcgattcgg
     2641 acggctctgg atgacgaggg attccaggag accgggatca tgtcctattc caccaagttt
     2701 gcgagcggac tctatggtcc tttcagggag gcagctgatt cgggctgtgg attcggggac
     2761 aggagcacct accagatcca cccggccaac tccagggaag ccctccgcga atccgagatc
     2821 gatgcacggg agggtgccga tatcctcatg gtaaaaccgg gaggattcta cctggacatc
     2881 gtctcctcgg tccgtgcact cggtctgccc gttgccgtgt atcaggtgag cggcgaatac
     2941 gccatgatcc gggctgcagc catgaacgga tggattgacg agaagaccgt ggtgatggag
     3001 agcctcatct gtgcaaaacg tgccggtgcg gacctgatca ttacctacta tgccgcggat
     3061 gcggcggggt ggctagatga agagcagtga ttatttcgaa caggccagga tgctgatgcc
     3121 cggcggggtg agcagtcctg tcagggctat taagccccat cctttctaca cgaagtcggc
     3181 acaggggagc aggatcgcca cggttgacgg cgaggagctc atcgattgct gcatggcata
     3241 cggcccgctc atcctcggcc atgcccaccc cctcatcaag gaggcgattg ccacccagct
     3301 cgggaaaggg tggctctatg ggacaccctg cccactcgag cccgaatacg ccgggatggt
     3361 catccgcgac caccccggca tggagatgct gcggttcgtc tcaagtggat ccgaggcgac
     3421 gatggcagcc atccgggtgg cacgcgggtt ttccggtcgc cctgacatcg tcaaggtgga
     3481 ggggggcttt catggtgccc atgatggtgt ccttgtcaag gcagggtcag gagcgacgac
     3541 cctcgggatc cctgactcgg caggggtcct tgccgatgtt gttgcccata cctggcaggt
     3601 cccatacaat gatcccgatg ccctggagag actcctgttc cggcacccgg agatcggggc
     3661 attcatcatc gaaccggtgc ccgggaacat cgggcctgtc ctccctgaga aagggtatct
     3721 ccaggaagtc cgcagaatca cccgtgccca tgatgtcctg ctcatttttg atgaagtgat
     3781 caccgggtat cggatcggaa tcggaggggc ccaggaatac tacaacgtca ggccggatct
     3841 caccacgctc ggcaagatca tcgggggagg cctccctatc ggagcatttg gcgggagacg
     3901 cgatatcatg gaacgtatcg caccggccgg gccggtatac caggcaggga cgttctcggg
     3961 aaatcccctc tcgctggctg ccggaatggc gaccatccag tggctgcatg cgcatcgcgg
     4021 catctaccgc gagctcgact cggccgggaa acggatcggt gaagcagtca ccggaaaagg
     4081 tgggtcattt gtccagcatg gctcgatgtt caagttcttc ttccggaccg ctccgccccg
     4141 gaactaccgg gaggtaaaag agtgcgacac cggccggttc tcctcgttct ggtcagccat
     4201 gctgaagaaa ggaatcttcc ttccgccctc gcagtttgag accaatttcc tatcggcagt
     4261 tcacaatgaa gttgatgtcg agaggataag cgacgcgtat aggtcatgcc tgtgaggatt
     4321 ggaaccaggg ggagcaggct cgcactggca caggcagagc gggtcgcctc cctgctacag
     4381 ggttgcggta ccgctccaga gatcgtcgtc atcaccacca aaggggatac cgttgacggg
     4441 gttcccctcc acgagatcgg ggggcaggga gtctttgtga gggcactcga tgatgccatc
     4501 ctcgacggaa ggatcgatgc agccgtccat tccatgaagg acatcccggc aaaacggcct
     4561 gccggcctct gcacctgtgc catactcaag cgcgattctc ctgcagactt cctggtccat
     4621 accattccaa ggaaagagat ccgggtgata gggaccacaa gcacgagaag gagggcacag
     4681 ctgatcaggt acatggaggg ggtttccgta aaacagctcc gtgggaacgt ggatacgagg
     4741 ctccgtaagc tggccgaaga gcagtatgac gccatcgtcc ttgctgaagc ggggctcgag
     4801 aggttgggac tgtcggtgtc cgggacccgg ctttccccgc agcggttcgt cccctccccc
     4861 aaccagggca cgatcgcggt agtgtgccgg gacgaagctc ccctctgcga agtattcgca
     4921 cccctcgatc atgaacagac ccggattgac gtgcagatgg aacgcacggt aatggaggag
     4981 gtagggggag gttgtttcac accgcagggg atctatgccc accatggcca cctgatcggg
     5041 gagatcctct cccttgacgg cacgagaagg atcaggaggg aggatgatgt cggctccctt
     5101 gaggatgcca ggacatttgg acagtacctc cggaaagaag gtgaggaatt gatcagggaa
     5161 gcatatgaga aactgaggat ttgtgatgaa gggtaaagta tatcttgtcg ggtccggtcc
     5221 gggaggggca gggcttcttt ccctccgtgc ccgggaagtg atcgattcgg ccgaggtgat
     5281 cctgtacgac cagctccccg gggaggagat cctcctctca ctgcccgagc atgctgaaaa
     5341 gatcgactgc gggaaatatg gatctgacca tacgctcgaa caggaggaga tcgaggagtt
     5401 gatggtccga aaagcacggg agggaaagag ggtcgtccgg ctcaagggag gcgatccctt
     5461 cctctttggc aggggcggcg aggaactcga ggtgatcagg gaggaaggga tcgaggtcga
     5521 gctcgtcccg gggatcacaa gtgcccttgc tgtccccggg gcggtcggca tcccgctcac
     5581 gcaccggagg tatgcatcct cggtcacgat cctgacaggc catgaggatc cgacaaaaga
     5641 ggaatcggca ctggactggg cgctgcttgc aaagcagtca gggaccattg tcatcctgat
     5701 gggagtgaaa aacctccccc taatcgccgg cctgctcatc cgtgagggaa aagatccgga
     5761 caccccggtt gccatcatcg agcgtggtct tcggaaagac cagcgggtta ccacggggcc
     5821 gctggcgaga ataggagccc ttgcaaagga acggcacgtc aagcccccgg cggtcatcgt
     5881 gatgggcggc gtggtgaatc tgtaccggga aggagacggg atcatccccc tctgaacagg
     5941 ccttacggcg atttatccaa gtattaatac cttaatacca ctgtacttta gtcaggtgcc
     6001 tgcatgagcc tgcccggcgg acccacaaag gatgagatca tggcgatctc cctcgcaaaa
     6061 ctctcgcttg agcccggcga tatcgttgcc gacatcgggt gtgggacagg gacggtctct
     6121 ctcgaggcag cacgcagggt tgcaaaggtc tatgcggtcg atctgagaga ggaggccgtc
     6181 tcctgcacaa gggatcgtgc catggcggcc gggatgacca atatcgaggt cagcctaggg
     6241 cacgggatgg atcttatcaa agctgtaccc catatcgatg ctgcgtttgt cggcgggtcc
     6301 cgtggacttg cgggaatgct ctccctcctc tcggagaaga aggtgaggtc ggtggtcgtc
     6361 aatgcagtac tcctctcgac cctccatgaa gcggtgacca cgatgcagga gttatcgatg
     6421 ttcaaagaag cgatccatgt ttcggtcgca cgctctgctc ccctgggtgg cgggctgatg
     6481 ttccggccgc tcgatcccgt atacatcgtg gtaggaggag ccccgtgctg atcgggcttg
     6541 ggctcggccc gggggaaccg gaatacctga cgctccgggc agtccggatc cttcgcgagg
     6601 ccgacgcggt gtttgtcccg ggcatgatcg caagggaact ggtagccccc taccgcgatg
     6661 cagagatcct tgacttcccc atgaccggag atgaagagag gatccgtacc tgcatggagc
     6721 agaatgccga tcgcatcgct gggatcgccc ggaccggaac agccgtgctc ggtatcctcg
     6781 gtgatccgaa cttcttctcc acctattccc ggctcactgc catcctgaac gtctgccacc
     6841 ccggtatccg gtgcaggacc gagccgggga tcagtgccat cactgctttc gcctcggtgg
     6901 ccgggatctc tctttccggc gggttccttg tcactgacgg cccggtcgaa ccacgatccc
     6961 ggatcatgct gaaggtccgg cgcccacgcc aggtagccga atcgctgaaa cgagaggggt
     7021 tccgatcatt tgtcctggtg gaacggatgt gcatggaggg gatgcagata tatcgggacg
     7081 atcaactccc cgaagagagt gactatatga gcgtgctgta cgcggagcgg tgaatgccgc
     7141 atatctattt tgtaggggct ggacccggcg atcccggtct gatcacggtc aggggtatgg
     7201 acctgctctc ccgggcggag gtcctggtct ttgcaggatc cctggtaaat cccgaactgg
     7261 tggaacggtc atccgcccgc atcaagcttg acagctgggg aaagtcactg gaggatatcg
     7321 ttccgctcat gatccgggag gcacagaacg gggcactggt tgtcaggctc cactctgggg
     7381 accctgcgtt gttcggtgca ataatcgagc agatgcagct cctcgaagag gcagggatca
     7441 cgtgcgaggt cattccaggc gtctcatcgc tgtttgcagc atcggccgcg ctcgggacac
     7501 agctcaccat cggtgggatt tcagataccc ttatcgtgac ccgccctgca ggaaaaaccc
     7561 tggcacatga tgagatccat gagctctctg cccatactgc cacgatggtc ttcttccttg
     7621 gggcggaccg cctggatgag atcacgggaa aactccagta ccccgcttca accccggcag
     7681 cggtggtata ccatgcgagc tggccggacc agaagattgt caggggaaca gtcgcagata
     7741 ttgcagggaa ggcagcagaa gcagggatca cccgatcggc gctcctggtc gtaggaggag
     7801 tccttgatcc atcgggatca ggccacagga ggtcgatcct ctactcatga accgggccgt
     7861 catttatctg ccacggttct ctgaggctgc cggaagaatt gcccgggtgc ttggtgccga
     7921 cctcctcgaa tattcggaga ccgctttttc gacagcaatt tcccgctacc gggagattgc
     7981 agcggtgatg tcgatgggga tagccgtcag gggaatcgca ccctacctga aagacaagtg
     8041 gacggacccg gcccttgtgg tggtgacgcc cgatctccgt ctcgccatcc cggtaacggg
     8101 aggccaccac ggtgcaaatg cactggcaag ggaactggcg gtactgggag ctgtccccgt
     8161 gatcaccact gccaccgagc gggaaggggt tccttcagta gagggaattg ctgcagctgg
     8221 gagatattcc ctggtcaaca gggacgcttc aaaaccagtc aatgcaggat tccttgacgg
     8281 gactgccggg ttctactcgg tgaagggtcc ggccgtggtc atcggggaca aggatacggc
     8341 atttctcatc acggatgggg aatacagcat cgggatcggg tgcaggaagg ataccagtgc
     8401 aaaagagatc gaggaagcgg tccggaccgt gcttgccacg tcagggatca ggcccgatca
     8461 ggttttcatc tatgccacga ccgcaaagaa aacacacgag agcggcctgc tcgatgctgt
     8521 ccgtgctctt gggcggccgc tcctttacct ggatgacctg accatcaatg ccatccccac
     8581 ccggtcaggg tcgcgggcat cccgcatcgg gctctcagga gttgctgaac catgcgccct
     8641 tgccacggca aaacgaagag aactggtcgt gggaaaaaca gttatcggag gagtcaccat
     8701 tgccattgca cggtaacagg aaggggatgc ttttcatcat cgggatcggg cccggcagcc
     8761 cagaccagat gacaggacag gcgagagagg cgctcctgca gtccgaggtc attatcggca
     8821 acgataccta cctgtcacag atcggggcac tcatcagcgg aaaggagata atccggagct
     8881 cgatggggca ggaagtggaa cgggcaaagc gatccatcga actctcacga tcacaaatcg
     8941 tcgcgatggt gagcgggggc gatgccggga tctacggcat ggctagcatt gtccttgaag
     9001 tcctcgagca tgaaccatgc agtgttccgg tcagggtgat acccggtatc actgctgcaa
     9061 acgcggcagc atccctgctc ggctctcccc tttccggcga ttttgcggca gtcagcctct
     9121 ctgacctcct cactcctctt gaggtgatcg aacggaggct tcatgccgcg ttctccatgg
     9181 ggacgccggt ggtgctctat aacccgaaaa gcagagggag accccatcat tttgcactgg
     9241 ccatggagat tgccgggaaa taccgggagg cggccacacc ggtcgggata gtgaagaatg
     9301 cctaccggga gggtgaggag gtcatggtca ccacgctggg agagattggg cacgccgaag
     9361 cggcggtgga catgcacacc accgtgattg tgggtggcga ggagagcagg atctggagga
     9421 acggagacga tgtcaaagga atcatcaccc cgcggggtta ccacaaaaaa tacgtatact
     9481 gaccttggtg caacaaccgc cgaggcatac gagatatcac ggacgagcag ggcgcttgca
     9541 caaaaggaga taggagatgc agactacgaa gaccgggtga ggcagcggtg ctccatcgcg
     9601 gtgggcgatt ttaccatggc agggctcctc aggttcacca aaaatccggt ggaggcaggc
     9661 cttgctgccc tctcccgagc tgcccctctc gtcacggata tcaggatggt ccaggtaggc
     9721 atccagaaga agggacatag cagcccggtt ctttgtgccc tcgattatgg agcagatctc
     9781 gccgaatccc gagagatcac ccggagcagt gccgggatcc tcgccctgaa agaccacctt
     9841 gctggatccg tgatagtggt cggcaatgcc ccgtctgcgt tgcttgccat ctgtgatctg
     9901 gtccgggagg ggtgccggcc ggctctggtg atagggacac cggtcggttt cgtcaaggca
     9961 gccgaatcga aggaagagct gcggagcctt gatatcccat ccatctccaa cgaggggacg
    10021 agaggcggga ccccggtcgc ggtcgctgcg ataaacgaga tcattaccat ccatgcagag
    10081 ctcaagcgcc agccctgata ggatcaccga tccggtgaca ggatttacct atcccggacc
    10141 atggatcgcc gcgtgcagcg acgaggaggg cctggatctc gtcagagcgg ggctcgctgt
    10201 gctcacttct tcagggatga tactcaggcg cggatatacg actggcacca cggctgcagc
    10261 ctgctgtaag gctgccacgc tctccctggc agaagtgcca ggcaaggtat ccgtcctcac
    10321 cccttcaggg ataagggtct gtgttccagt cgaagccacc tgtggcattg catcctgttt
    10381 taaatatccc ggggattatc ctggggatgc cactgccgga atccagttta ttgctgagtg
    10441 caatcctgcc gacaacggga tcagcctcta tcccggggag ggtatcggac gctttacaag
    10501 ggatacccca aggaatcgga agggcgaccc agccatcagc tttcctgccc tttcctctat
    10561 ccttgccgcg attcaggaag ggctcgaaac aacagggctt ccaggagcaa gcatccatct
    10621 ccgggtacca cggggggaga tcgttgcgaa agacacgctc aacccgaaac tcggaatcga
    10681 gggagggatc tcgatccttg gcactaccgg gctcgtcgag ccatgggacg atcacctcac
    10741 aaagaccgtg atggaacggg tccggaccgc tgaccgggtt gtgctcacga ccgggaggac
    10801 cgggctccgg tatgcccgcc tcctcttccc cggttacgaa gcggtacttg cagggtccca
    10861 cctcagggag gcgctgggtg aagcaaaggg acaggtcatc ctctgcggac tccctgcact
    10921 tatcctgaag ttcctggacc cggagatttt gagcatgacc ggatttgata ctgtcgaaga
    10981 atttaccaca cggccggagt tcagggcacg gatgcaggcg tcatttttaa aggggaagga
    11041 gcagtatccc cgtctgagag tcgtaattgt cgatcgcaac ggaactatca tgggtgattc
    11101 ggggtgaaag tcgtcggggt aggctgtggg cctgggatgc tcacggaaca ggcagcaagc
    11161 gcgatctctg atgcggcgat catcttcggc tcgaaaaggg caattgccct tgtggcagcc
    11221 catatccctg caggctgcat cgtccgtgag atggtcacgt tcagcccgaa ccagtcgatc
    11281 cctgataacg ctgttatcct ctcaaccggt gatccgcagc ttgccggcct cggatatctc
    11341 ggtggtgaga taatccccgg catctcatcg ctccaggtag ctgcagcacg acttcatgtc
    11401 cccctggaac aggtatccat cgtgatagcc catggcaaag atcatggccg ggcgcttaac
    11461 aggattgcag acgaggtaaa acgcggacag aacaccttca tcatcgcgga tccaaagttc
    11521 tcaatcaccg cgttatgcac agcgctcgag ggagcaggtg aagacatctc tattgcggtc
    11581 tgtgaagatc ttggctatcc tgatgagaag atccggatcg gatcaattgc aagtcctcca
    11641 cagccggtct ccccgctctt ttcggtcatg gtcggcagga ttgaaagccc ccctggggac
    11701 cagaagagcc actgaagggc atatatggaa gcaccgccta cctcttttta aggaaggaag
    11761 gtgaaccatg gagaaagcaa caaagaaaca attgagcctg ttctgtttcc tggcaggttt
    11821 tattctcgta ctcatccagg atctcaaggg attttccacg atcgatatgg gattctcctg
    11881 gccatgggca atcatgttct acaccgggct tctgctcatg atgatagggt attatctccg
    11941 gggttgaacc gggtccgggg atcactctgc cacaatccgc gttatagtgg ggaggtaccg
    12001 aactgcaggg aatatcaggt aatttttacc atatccctgt ccagggctat gaatccctcc
    12061 ttggcgagaa cggcaaggac tggatcgatc tggtcctgag gaaggccggt ctccctgcag
    12121 agttcttctc ttgtcagtgt tccgtgtttc acgaggagtg ccaggatctt tcccctcacc
    12181 tggcggacag acccctcgaa cggtgattgt tttttatact gctggctccg gtggttcgga
    12241 ttctcgtttg ttctccccag gtaggcgcca taatccatca gggcatagta ccagtccctt
    12301 ggattctccc tgtcaagggt ctcttcaagc agggggagga tctccttatc tgtcactacc
    12361 gggcgatcat ggaagaagca atgaataaat actctccggg tgttcgtctc gaggagcaac
    12421 gacggttttc cgaacgcaaa tgcacggatg gcagctgctg ttgcctttcc gattcccgga
    12481 aaggagagga gagcctcttc atcgtcaggg aggaccccgg accatccgtt catgatcagc
    12541 gatgccgtcc tcttcaatgc aagggcacga cggttatacc cgagaccctg ccactcgcgg
    12601 agcaggtcct caaccggagc agcatggagt acagcaaaat ccgggaacct cgtaatgaat
    12661 ccgtcatatt tttccagtac acgtgcgacc tgggtctgct ggagcatgaa ttcagagacc
    12721 aggatccgat agggttcgcg ggtctccctc caggggagat ccctccggtt tgcccggtaa
    12781 aaatggtaga tcctgtcccg gaactcgccc gcgtactggc gggtcggctc aggtctttct
    12841 ctcatcacga agcccattga tggtggcggc ataccggccg gcatcaaatt tccggttcag
    12901 gccggcagcg ttcatatacc gtattgttcc aaaaacaggt cgttctttcc agggccggtc
    12961 atgcttgcca aaacaccacg ctacccctgc atacccgttc ggatcgcgcc cgtccagttc
    13021 gtaccggtta ttgagggtca gggcggtctg gtacgcttcc tcaggggtct cagaccattc
    13081 gaggatcttc tttccccagt acatccgcat atacccatgc atcttcccgg tgatcaccat
    13141 ctcctgctgg gctgaattcc agtagggatc atgcgtccgg gccgattcaa acgttgagaa
    13201 cccgtacgaa tactcacgcc ggtcttccga atggacggaa agggtctttt ttgcccaggc
    13261 agggagccct gcgtaatcat catacagggg gttgtaatgg acaaaattta tcgcgagttc
    13321 tctcctgacg atcagctctt caaggtaagc cgctgcagaa aggctccctg accggaggat
    13381 attctgtgca ataaagacgg gagagatctg gccgaaatgg aggtacgggc tcatgcagga
    13441 gagcgccccg gccgatggat cattccgctc tacgggataa tggtcgagat gccgggcaat
    13501 gaaccgtttg agaagggcgg cggccgcttc ctcaccacct ttccagggta gcggataggg
    13561 ctgcagggtg ccgccctgtt ccgggattat tcccggaatg ccggaaagca gcgattcaac
    13621 ggtcatctct ctctcaaaac cccgctcaag gcctagggat gatcgtgatg gttttttttc
    13681 ttccagcggg agaagaaacc ggtcgatgtg aggggtgatc ttccttctga acgttgcagc
    13741 agaccattcc tctttttcgg atgcgatgtc tgccggaacg actacattgg tttcgacacg
    13801 gaccaggaga caatcgagct cccctgccac ctgctgttgc cactggtcct ggaaccgcag
    13861 gtagcccctg tcggtcacca ccagcgccgc atctcccccc agttcccgtg cagaggttcc
    13921 aggaggacct gtcttgacga tgaatcgcac cccctgcgct tccagatcgc gggagagggc
    13981 gatcagccct tcgaccatga aacggtagtg cgggcatgtg gccccgggat aaccgggtat
    14041 caggtcaaaa caggcaagga gcggaagcga atggcgttca gccatcctga ccgcgtactg
    14101 gtacgcatgg ttgcaggtga tccgtggcgc tgcctgcatc caacaaagga tgtaatgccc
    14161 tttctttaca gattttttcg agagatacgc taccctctct tccgggatca ttgcctgatg
    14221 gttgacgttc ttcctacatt accattctct gttatttacc tggctaaaag agggttaaga
    14281 atgatctttt tatctgctga acaattgtct ccaggaaata gggggaactg ccccggtcct
    14341 ggtatagtcc acccacacct tctccggatc tccatgaagg gtgatatgtg aacgccatga
    14401 aaggccggat gggttcacgg tcgtaaaggt atcggtgaat acggtctttt cccggcttcc
    14461 gatggtagtc ttcgtaaacg ataccgtctc gttcacggtt ccctccacag aaacgctcgt
    14521 gctcgtagag attggaccac tccagggtag atctgctgca acggaccctg caataaaaat
    14581 ggcgagcagc atcagaatga tggttgcttt tattccttcc ttcatatgac atactcccat
    14641 ggttccggga aggaatcatg ttcagtggaa agaagaaccc cggcactata taatcttact
    14701 gaaccacccg gttcaatcat ctgaatcaca cccttttaaa gaggtcatcc atcttctgtt
    14761 tccggaacac gatcatcgtt ggtctcccat gagggcaggt gaaaggattc ctggtccggg
    14821 caagctgctg aagcagctct ctgcattgat ccagggtgca gaacgcccct gccttgatag
    14881 ccccccggca ggcaacaata cgcctcacct gctcgcgtgt atcgatgccc cttggcatcc
    14941 cggggcctat gatcccagcg ataatatccc gtatctctcc gggttcgata ttctgcccga
    15001 agaggacggg aactgtcctt acgagaaagg tcccgggacc aaatgcatcg atacaaaatc
    15061 cctcttccgc cagaagcggg agagctgcgg aaagcgcctc gctttcgccg ggtgagagtg
    15121 aaagaaaaac aggctccagg agctcctgga gatcatggtg ctgctcggcg tattccgtga
    15181 cctgttcgta gagaacccgc tcatgggctg catgctggtc gatgaccagg aggtcatccc
    15241 cgggagtgcc tgcaaggatg tacaggctcc ctagctggcc gataatttga aaatcgggga
    15301 gtttcgattc aactgaggta tccggctgtc taccggtcag gctgagctga cgctcagtgc
    15361 tgatccgatc ccggaggagc ggttctttca ccccggtctc cccacccaga tctggcagac
    15421 ggtaacggat tcgaccggga tgttctgccc cggaaacaga atcgccactt gaccctgctt
    15481 ccggtataag ggatgccccg ctaaggctct cgcttatcgc tgatatcagg acctttctga
    15541 cctcctcctc ccggctgagc ctgacctccc gcttggcagg gtgcacgtta acatcaactt
    15601 ctgatggatc aatgctgaga tcgagaaata ccaccgggta cctgccggtc gggaggaggg
    15661 tgccatatgc ctccctgact gcatttgaaa ggatacgcga gctgatactc ctcctgttca
    15721 ccgagatgaa catctggtac tgattctttc tcgtgagttc agggaccgag atgtatccgt
    15781 caacgaaaat tccctcactc tccgcatgca ccctgatcat ctgccgggat acgggaaggc
    15841 cgaaaaggtg gatgatagta tccatgagtc tgcctcctcc aggtactgaa aggatcttcc
    15901 gatcgttgtg agtcagttcg aacgctatcc ctggatgagt gatgacaagg cgctcgatga
    15961 atccggtaat gcgtgcaatt tcagatgata ccgttttttg aaatttctcg cgtgcaggtg
    16021 tgttaaaata cagctgttcg actgtgatca cagtcccaaa cgggcatcct gtctcagaaa
    16081 ccaggaggat ctctcccccc tggttcacga ttcgtgtccc tgatatccct gccccttcgc
    16141 ggctcttcgt gacgatggtc accttcgcca ccgctgcaat gctggcaagc gcttcgccgc
    16201 ggaaaccgag agtggaacag gcgaacaggt catcgatgca ggcaatcttg ctggttgcgt
    16261 agcgggcaaa agccagttgt gcatcgtccg ggctcatgcc acacccatca tcactcacct
    16321 ggatcgaagt gatccggcct ttctccgatg tcacagcgat cctgatccgt cctgcaccgg
    16381 catcgatcga gttttcacag agttctttta ctacggaggc tgcccgctcc acgacctccc
    16441 cggccgagat ccgggatacc gtgctctcat cgaggacctg tatccgggtg ccctgaccag
    16501 gcgtcatcgc tcagtcacgc ttcctctccc ctgcccgctt tttcaattcg tagagtatcc
    16561 cgagggcatc ccgtggggtc aactgatcag gatcgatctt tttcaggtcg ctgacaacgg
    16621 gatgttcgac gagagggggt gcatcgacaa gcaacatctg ggtatatcgc tggagcctcg
    16681 gtccccctcc tccttcacca cggataaggt cttccatgag agccgacgcc cttgtgatca
    16741 ctctccccgg taacccggca agtgaggcga catgaattcc ataactccgg tcggtagccc
    16801 caggtatcag tttccggaga aaaatgacat ccttgccggt atcctttacc gcaaaatggt
    16861 aggtacggac cctttttaag tcatgctcgg ctgctgctat ctcgtgaaag tgtgtagcaa
    16921 agagggtttt tggcccggaa gccccccttc catgaagaaa gtcaaggact gcccgtgcga
    16981 tgcaataccc gtcaagagta cttgttcccc tccctatctc gtcaaggatg actagacttc
    17041 tcggagtcac attgttcagg atgtttgcaa gctcgatcat ctcgaccatg aaggtgctct
    17101 gtcctgacgc aagatcgtca aatgccccga cccgggtaaa gatccgatcc acgattccaa
    17161 cccgggcatg ggctgccggg acaaaagatc ctgcctgggc catgaccacg gtgagcgcca
    17221 cggatcgcat gtaggtactc tttccggcca tgtttgctcc cgtgatgatc aggatctggg
    17281 agtcacttga agagagtacg gtgtcattgg ggacaaaacc gcctttcata ccgggttcca
    17341 caacggggtg cctgccctcc cggatcagga tatcctgcgt ctcatcaatg acagggcggg
    17401 tatactgttg cagaacggca gcctcggcga gtgccgaagc agcatcgatc gcggcaatac
    17461 cccgtgcgac cgactggagg gaggggacac catcattgag cttccggagc acatccatgt
    17521 atagttcctg ctccagcgag gagagccggt cttctgcagt tgcgatgagc gcttcctgct
    17581 ctttcaattc cgggattgtg aaccgttctc ccgatgcggt ggtctgtttc ctctggtagg
    17641 tcgggggaac aagggcgagg ttcggtttcg tgacctcgat ataatagcca aatatcgagt
    17701 tatagccgac cttgagcgat tttatccctg tcttctcccg ttcctgttgc tggagggtga
    17761 tgatccattc ccggccggat gcggccctgc tccggagttc atcaagcagg ggatggtatc
    17821 cctcgcgtat caccccgcca ttccgggcaa gggctggcgg gtcatcggca atggcgttca
    17881 ggatcagggg agcgatccat ccctcgtcaa cgagatcccc gcacgaaccc gcgagttcga
    17941 ccgggaggga gggatcgtcg agggtttccc ggacggccga gagctggccc agcgtgcgtg
    18001 ccagggcaat gagatcgcgg ggacctgcgt tggtgcatgc tatcctaccg gcaattctgc
    18061 tgatatctgc cagctcaccg agtatctcac ggacccttgc cctgacggcc gtcttcctgg
    18121 agaagtattc aacggtgtcg agtcgccggt tgatagccgc aggtgatacc agcggtacag
    18181 cgagcatgct ccggatgagc cggcttccca tgggggtcat gctccggtcg aggagggaga
    18241 gcagggttgg atcccccgat ctcccccgga tgctctccag gatctccagg ttcctgagcg
    18301 tgatcccatc gatcaggcat gcctctcctg cctgccggta gaagatctgt tgcacatgcg
    18361 tgagatcaag ccgctgagtt tccttggcat aacagagtac catggcggct gccataacga
    18421 cggagggggc tgattctata cgaaagtctg acagatcgct cactttgaag tgatcgataa
    18481 ggatttttct tgcgctggca ggatcagtcg cacagtttcc agctgcagtg accaccaccc
    18541 cggactgctt tacgatatcc ccgagttccg ccgagaggta ttccggcatc agacattcgg
    18601 agggacgata ccgtgtcagt tcagacatga gggcagcggt cccgcgatcg gaaggacagg
    18661 ttgtcacaac aaactctccg gtggtgatat caagaaaggc caggcccatc acagaattcc
    18721 tttccaggga cgccaccgcc atcaggtagc gggcaccgga tgatccgatc atcccctcat
    18781 cgatcaccgt ccctggggtt attattcgca ccacctcgcg ccggacgatc ccttttgcat
    18841 tctttgcatc ttcgacctgg tcacagatgg caaccttgta gccttttgca atcagccggg
    18901 cgatgtatcc ctcaacggaa tggcagggaa caccggcaag cgggatcttc tggccgccgg
    18961 aatctttcga acgggaggta agggttattt cgagctcacg cgagacggtc tctgcatcac
    19021 cgccgaatgt ctcgtagaag tcaccgatgt ggaaaaggag gatagcatcc cggtactgct
    19081 ccttgatcgc ccaatattgg gccatcgcgg gcgtcaacct gccatggttc ggtccgggat
    19141 gctcctctcc atttgccatt atacagatga tcgtagtgcg atgagaaatg cataccgatc
    19201 cagaacacgg agcaggagag tccggggttt cagcctcatg gtaaatacaa aaaaattcgt
    19261 gaaataggtc acctcaacta ccaggcctca ccagatcgta gcaggaacag gatcccatac
    19321 acaagggttg aaataggcag gaccctcaga cgatgagcat tggatattga tgatggagcc
    19381 agaactacga ggggatacca atgatcggca ggggagaaag agaccgaacc ggcttatcaa
    19441 cgaggtaagc ccctatctgc tccagcatgc ctggaacccg gtggaatggt atgcatgggg
    19501 agacgaggcg tttgcacacg ctgccgcgga ggataaaccg gtatttctct cgatcgggta
    19561 tgccacctgc cactggtgcc acgtgatgga acgggagtcg ttcgaggacc ctgatgtggc
    19621 aggggtactc aatacccatt tcgtatcggt aaaggtggac agggaggaac ggcctgacat
    19681 cgatgctctc tacatggcag caggccagat gctctctggg atgggaggat ggccgctgaa
    19741 tgtgttcctg acacccgaaa aacaaccgtt ctttgccatg acctatatgc ccagggagcg
    19801 gcgttttgga actcccggta tcatcgatat tctcgaggag atcagcagac tctggagtga
    19861 agcacgtccc gagcttatca gaacggcagg actcacggct gcacacctct ccagggagac
    19921 gggaaaaggc aggaggttac agcgaacgat acttgatgct gcataccatg agctccttct
    19981 ccgttttgat gtgagagatg gcggatttgg gggcgctccc aagttccccc tcccccacac
    20041 cttgctgttc cttctccggt attcacaact gaagggcgaa gagcgggcga taagtatggt
    20101 tgaaaagacc cttcgttcca tggcgatggg tgggttgtat gaccagatcg gttctggttt
    20161 tcaccggtat tcaaccgaca cccactggct cgttccccac tttgaaaaga tgctctatga
    20221 tcaggcactc cacgtccttg cctatacgga ggcgtaccag ttgaccagca acgaatggta
    20281 tggaactatt gccagggagt cgctggaata cgtgctccgc gagatgaccg ccccgcaggg
    20341 aggattcttt tcagcgcagg atgccgacag tgggggagaa gagggagggt attatctctg
    20401 gacgaagaag gaggtggagg acctccttga cagggagaca gccgcagctg catgccgtgc
    20461 ctggcatgtc actaccgctg gaaactatat cgatccggtg agtggggaga gaacagggag
    20521 gaatatcctc catatcacgc gtgaaataac agaccttgcg caaggtatgg gattctcccc
    20581 cgaaaagctt acatctctca tcgagacagc caaaaaaaca ctctttacgg cccggaagaa
    20641 ccgcgccccg cctctcacgg atgacaagat ccttgccgac tggaacggcc tcatgattgc
    20701 tgctctcgca aaaggtgccc ggatctttca cgaagaacgg tacaggaacg cggcagccag
    20761 ggcagcggaa ttcattctcg ggtcgatgaa gaatcctgac gggcagctcc tccaccggta
    20821 ccggaacgag agggcgggga tcactgcaca ggctgcggat tacagcttcc tcatctttgg
    20881 tctcacggag ctgtacatgg caacgtttga tcctgcccat ctcgctgctg ccctttccct
    20941 gcaggaatcg cttgacagcc agttctggga tcccgcaaac gggggatatt ttactgctgc
    21001 agggaccgag accgacctga ttgcacgcca gaaagagttc tatgacgggg caatcccgtc
    21061 ttcaaattcc gttgctttca ccaacctttt gaggctcacc atgcttaccg ggaatacctc
    21121 gttcgaaagc cgcgcttcgg atctttcccg tccctatgct gaacacctcg cacaggcacc
    21181 cgctgcctac ccctttttcc tgagtggact ctgcctcctc tttggtccct cctctgaaat
    21241 cgtcattgcc gggggagaaa aaggtgatac ggcagaagag atggtggatg cgctggaatg
    21301 cagatacctg ccttttaccg tgatcctggt aaaagattcc aggaatgagg ctgcgctggc
    21361 aaaggctgcc ccgttcacca gggacatggg tgctatcgat ggggagactg ctgcatacct
    21421 ctgcagccgt cactcctgct cagtacccgt gaaaggggta aaagaactgc tcctggggat
    21481 tgagaaggaa aaagaccgga aaaaatcctg ggtttaaaaa aatattcaga gattccgcaa
    21541 tgctaccaga tcctttaccc cggtcagtct ccatacaagg gatcgttcct cgagggcaat
    21601 ggcctccggg ggatcctggg gggaatggcc gagggctgcc aggatattgt ttgagagctt
    21661 ccgccccttg ataaggttca caaaatcctc gcggatgatc ttcttgtcta tggaatcggt
    21721 cacttcctca aggtagccct ggagggtgat gaaggtgtag caggagaggt cgcgtgtgta
    21781 cttctccacc tcgaccgcaa catgggggct cttcctgaaa tactcgatct tctttccata
    21841 ttttgtcgaa aggaaatgaa ggaacttgcc atcgaagaca tagaggaaag gtgcaatgta
    21901 tgggtatttc tctccctgga atgcgatgcg gcagacatac ccttcctcga taagatggtc
    21961 atagtcctgc ttttccatgc ggggaatttt aacaatgtcc atacgatttc ctactctcct
    22021 caatagttgc gggggttaca taaatttttt taattcgagt tcgtgcatct gatcatgagg
    22081 ggcttttagt atcgtccttt ttagaaatca tccttttaat gaggcctggt ttcgaaaaaa
    22141 agcaaaaaaa ggcatccttc caaatcgcgg gaccattcag ttcgtgtatc acgaatataa
    22201 ttcaggcggg tcccatggag gggagccata gtgcagaaga aggctcacct tccaggagcg
    22261 cagatcctga tccccttctg ccgggcttga gctgaatcac aatgatgccg ggatactgta
    22321 atgcgagagt gatgaaatcc gcttttctgc tgaaacatgg ccttccatga ttcgagtgaa
    22381 aggttcccgg attggtccat cacgcttatt gtacgtgctc cacgggatat cctgcccgca
    22441 gatacgcccc cattccgcca aggacattgg ccagtcgcaa ataccccttt cctgcgagat
    22501 aactcgctcc aagcgaaccc ttgaagccgg catcgcagta gactgccact ttacgatcca
    22561 tcggcacttc ccccaccttt cccgggagtt ctcctacata gatatggtgg gcttccggga
    22621 tcatgccata cttccgcaca tttgcaatat cacgtacgtc gaggaggaag aggtccccct
    22681 ttttacgctc tcttgcaagg tcatgcacac tccaggcttc gaactgccct atagggcttc
    22741 ctgacttgaa atatgccgga aatccccctg caaggtaccc ctccaggtta tcgtacccaa
    22801 gccgtaggag atgcctgctc acttcccgga gatgacagtt gaaatcgtcg acgagaatta
    22861 ttgggtgatc atacgaaagg aaccatcctg caaaaccagg gataccatcc ttccagatcg
    22921 agaggctgcc cccgatgtgc cccccggcaa aggaggtggg tgaacggatg tcaagcacct
    22981 gggacttttc ctggatatcg cggagttcat ccaccgtata gggacgaaga tccgggagat
    23041 gggagagaac cggggctccc tgctggttca tctgttccat cttcgcaaag taaggaggaa
    23101 gataatgatg ctcgttcacc ttttgttgta tgaactcacc ttgagagaga gagagatgag
    23161 gatgtgtctg cttttcatac cctatcgtgg taacctcatg gtcgctgatc gcggatccgc
    23221 acaccgaacc tgccccgtgt gcagggagca cgatcgtgcc atccccaagc gggaggatct
    23281 tcttccagat gctctcgaag agcagtgctg atacttcagc cctcctttca gggccgaaaa
    23341 aatcggtgcg gcccgtatcg ccggcaaaaa gggcatcccc ggtaaatact gcatagggga
    23401 ggtccgacac ctccctgtca gtcacaatga tcgatatact ctcaaaggtg tggcctggtg
    23461 tctcgatgat ccggagctca attggtccaa gcctgaacgt gtcgccttcc ttcactgcgt
    23521 tcccatacgc aaatgatatc tggctgccat ggtagatggc ggctccggta cgggatgcaa
    23581 gttcgaagga cccgatcacg tagtcttcgt tgcggtgggt ctcgaatata tgggtaatcc
    23641 tggcatcgag ctcccttgcc agttcaaggt atgcttcaca gtcccttctt ggatcaacca
    23701 ccgcggccat tcctgccgca ctgatgaaat atgagtaatg agagagccct tccgatggta
    23761 tcttccggat gatcattatt acacccgttc ccgaattgaa ttccggttcc aatatagttt
    23821 tcgaacaggg agattcaggg gcgtcaagct aattgctgaa tcactcctac cttgaggcgg
    23881 atatgactgg tctgttcaag acgctcctct tcgatatgga caacaccctc tttgaccttg
    23941 tcaatgcaaa gatcgaggcg tgcaaggcgg tgacagcgag actcgatgct ggtgatgccg
    24001 aagacctctt ctcatacttt ctccgccggc agggggggtt tgatgatcct gaaaatatcc
    24061 gcgacttcat gctggaccac tcctgctaca atagaggcct cttttgggag tgcatatcgc
    24121 tctaccgcga gatacagacc cgaaacctaa ctccctaccc tggggtggaa cagacccttg
    24181 cactccttgc ctcatcggga ttccgattag ggatcgtgac cgatgcatgg ctggcagatg
    24241 caacaacccg tcttgaagca accggccttt caccgttctt cgatcacgtg gtgacccatg
    24301 aaatgaccgg ggcacacaaa ccaagcagtc tcccgttcct ccttgcactc gtccggttga
    24361 atgccactgc acgggagacg ctccttgtcg gggacagccc gaggcgggat atcgcccctg
    24421 caggcatcct gggcatgtat accgtgtatg ccaggtacgg ggaccggttt tcccacccga
    24481 agtctgacgg tggtgcggac catgccatcg attctttccc tgaactactg cctatccttg
    24541 aacctggcgg ccggaatgcg aaggctgcaa ggcaggctac gctcagctga aacaatcccg
    24601 ccaccatatc cgggaactat atcagaacct gataccaaga ggtaaccgga cgatatgacc
    24661 agaaaacgtg ttcttttcat ctgttcccac aatgctgccc ggtcacagat ggcggagggt
    24721 tatctccggg ccaggcacgg tgaccgcttt gaggcattca gtgccggaac aaaggcatcg
    24781 acgctcagtc cgtatgcgat ccgggtcatg caggagatag gagtggatat atccgggcat
    24841 cggtcaaagt ccctgaatga aattgcaggc agggagatgg atgtggccgt catcgtctgc
    24901 gatgcagcaa agtcagcatg cccgttcttt ccctgggcaa aggtgaccct gcatgccatc
    24961 ttcaggaatc cgaaggaatt tgcagggacc gatgacgaga ttatcaacgg attccgggaa
    25021 atccgcgacg agatcacggg atggattgac cgctttgtct ccgaaaaatg aactgaatca
    25081 cacaggcatt tccggagggt tcaccgctca tccggactat tcagagagcg cgatgccgga
    25141 aggctctttc ttgtcgcttt ccggcataaa tgccttgacc atcgcttgtt ttccagcttc
    25201 acagggctat ggcagatagc gatctgctcg tactgtcata caatacaccg tggcattgtc
    25261 aggcactgtc acatcattca ggtgaagaat accgttcgtc tttttgtacg aacgtcgttc
    25321 atccatgcaa ggaggagaaa accccctttt tcccaaccat ctagccacgg accttttgga
    25381 gggtttcctt tacctcctca acatggccgg gcactcttac cttccgccat accgcggcga
    25441 tggtgcctcc gggagatatg aggaacgtgc tccgttcgat ccctttgagg aggacgtggt
    25501 ctttccatac cttgtatgcc gcaatggccg catgcttctg gtcggagaga atacggaagc
    25561 ggagccccaa ttttgccgtg aatttctgat gactggcctc tgagtcggca ctcaccccta
    25621 cgatctcggc accgagccgt gcaaactctt caatagcttc agagaagcct cgggcttcga
    25681 gcgtacaccc cggagtgtta tcccttgggt aaaagtagag cacgagccac tttccggcaa
    25741 aatcggtcag gcagtactct tccccttccg cgtcagggag gcagaactcc ggagcaggcg
    25801 aacccatcat tggatcgctc ttctggtctg tcatgatatc cactgttctc tgatatgtca
    25861 ggactgataa aaaatataca ggactcccac aatcccatgc cactgaactc cccacatcct
    25921 atgccaccga attttcaaga tattatgcat tttcctgata ttcctcatgt gcctggcaat
    25981 tgtatgattg attccatggt tgagaaataa aggagttgca gggaaaccgg aggaattttt
    26041 ccctctttca agaagaaaag ggtgaagatt ttattttcct ttcctgaaaa aaccgaaggg
    26101 gtcacgataa ttgaaaagca gccgggactt cctctcggga aaaactcctt ctgccaccga
    26161 cttaatctcc tcgaccgagt agaaggttcc cggcagatgg tgctgaatcc cccggacaac
    26221 ctcttcgaga tcctggcgct tgatgaccga gaggattatc ttcaccttcc ctttcgatcc
    26281 ctccccgtca gcgatggtaa ccccgaagct gtgtgctctc aggaagagga gaagccctga
    26341 aatatcctcg tttgagatca cccggacgac caccgtccca atagaaagac gttcttcgac
    26401 agtcatccca agaag
//
LOCUS       DAOOCO010000006        26266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_1538_length_26266_cov_5.731258, whole genome shotgun sequence.
ACCESSION   DAOOCO010000006 DAOOCO010000000
VERSION     DAOOCO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 26266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26266
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1538_length_26266_cov_5.731258"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..478
                     /locus_tag="PK154_00785"
     CDS             260..478
                     /locus_tag="PK154_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09629.1"
                     /translation="MSGDPPLLRCPSCGSLLTSAIGDTGMWECHYHLCHAVFPAFISG
                     EAFQDAQVVAEATPPYLPGDQQHRSKSL"
     gene            complement(1067..2413)
                     /gene="purB"
                     /locus_tag="PK154_00790"
     CDS             complement(1067..2413)
                     /gene="purB"
                     /locus_tag="PK154_00790"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW09630.1"
                     /translation="MAIHPIDYRYGTPEMRAVWTEENRFACIVRAEAALARAGADFGL
                     VPEAAADRIGENAKRASLSRAKEIEAEINHDMMAIVKAIAEVCGESGGWVHYGATSND
                     ILDTATGLQLKESLGLLDAKLRNLLSILLKRSHDTRHLVCAARTHGQIGVPTTYGLRF
                     AIWAAETARHLDRLSELTPRVAVGQLTGAVGTQAALGEKGMEIQDRMMELLGLVPVAV
                     SNQVIARDRYAEYFMFLANVATTLDKIGIALRTLQRSEIAEVEEAFGKKQVGSSTMPH
                     KRNPIRSEQVCGLARVIRSAVEPALQNNTLWDERDLTNSSCERVIFPEATILADHCIS
                     LLTRVLEGLVIRQENIRKNLDLLHGVNMAESVMIALAGKGMDRQQAHEMVRVASMKAL
                     AEGKPLDEVLAQDPATARWLSRQEIHDLLNPDRYTGTAVQQVERLYTNLKRNYLNG"
     gene            2471..4477
                     /locus_tag="PK154_00795"
     CDS             2471..4477
                     /locus_tag="PK154_00795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPW09631.1"
                     /translation="MENPDAYFPYESYRPHQKEMLASVAACARQGGIAMIDAPTGSGK
                     SSVVAALLAERSGRKIFVAVRTISQLNTFIRELELIRKKKRPISYSYLIGKRSLCMLP
                     GQGDIYRRCEGVKAVSTSLMRERAEKGSLVPSKDPVITQQIRKLDEEHPVICPYYIRS
                     RVFLTGEGSGLRMVPSTPLKAKAERVSRELARPGELRDIGAGICPYEMLLLAAQRSDV
                     IVVNYHHIFDEDIRNQLYGSLGIEPADAMLLIDEAHNCGEVMTGIMNVVVGEQVLAQA
                     DREIAAIRRHLKSTAAIGQIISNIALFMQGLKNSHETEDWFDPAIFDRMVVRGSLYKD
                     MAEVVEDLMKISEYIREKNMMAGEYSMSATEMLTAFLVRISCSSTDPGYLTVYRKEEP
                     DIFLEVRSIDPSDRLRDLALSHHCTVLISGTLAPLESYQRYFFGDLPVTGCSLPNTFP
                     KKNRMVLCAGDITTAFSMRENRENSARVAQYIQHFARLKGNIAVYFPSYQILDTYARL
                     LAAPLASRNLIIEPKDARDAGEALTTFLSLPSKGKSGILFAVAGGKWSEGLDYRGELL
                     SGAMVIGLPLAPFNQVRKMTIDYFRHKFGPDGEFISYTLPALNRAEQAIGRVLRTPED
                     RGILVLGEKRFLEPRVKAGLPGWIREEMVECTAESFGEVMKRWK"
     gene            4468..4929
                     /locus_tag="PK154_00800"
     CDS             4468..4929
                     /locus_tag="PK154_00800"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW09632.1"
                     /translation="MEVIEGSARFGLWYVHVRWSGTTVYQVRFSRTPVRGPVPVPITQ
                     YLSGRIVDLSTLTCGSPPAGEHYARIYGEVQRIPYGETATYGEVAEWSGTTARVVGQA
                     LSRNILPLIIPCHRVVARDGIGGFTPDPAIKVDLLAMEERSKRQSRRRTED"
     gene            5075..5671
                     /locus_tag="PK154_00805"
     CDS             5075..5671
                     /locus_tag="PK154_00805"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPW09633.1"
                     /translation="MRSAIVLVGGEARRVNGMEKYFFRYLGKTFIERLIDALRPAVDE
                     IILVAKDDEHCERFAHIYEARCVHDIRKGMGPIGGLHAGVAACKGDPIFVCACDMPCI
                     DTMVVSALFDALGDYDAAIPSWDTEMLEPLHAVYRKDPLQHYLEDPPSLSLRSLVKHL
                     NVTFVPVGRIQAIDPDLSTFTNINKLEDLEYLGREDSD"
     gene            complement(5756..6493)
                     /locus_tag="PK154_00810"
     CDS             complement(5756..6493)
                     /locus_tag="PK154_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09634.1"
                     /translation="MRTGIICILVIVAMVCLSGCISPPEKNTGSAAQGGTAASKGGIS
                     GAKATPTAAGTDGGSTTTTTPTPAPGEAGYLVPATPYPTEAIPASTMSGTRLPDITPS
                     PVEYMGIYYSTLALKNNRTAFTYDLQKPPLVIEICFSPNMTTRTIWYDSKYTDRGEKT
                     ETVTTISPAAWFEVTVREPASGQIVAKEGFGRGYSTDTGKDLTIRSQGKYLIEFSGNE
                     LSAQVQIRVPKEGNPAGSPLKKMACSF"
     gene            complement(6617..7165)
                     /locus_tag="PK154_00815"
     CDS             complement(6617..7165)
                     /locus_tag="PK154_00815"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPW09635.1"
                     /translation="MAVPSKIFFTKGVGVHKDRLASFELALRRAGIEKYNLVYVSSIF
                     PPFCRLVTVEEGARCLEPGSITYCVMAKNETNEPNRLISAAIGLALPKDSQEYGYLSE
                     HHAYGETAEKTGDYAEDLAATMLATTLGIEFDINQAWQEREQIYKASGKIIKTTNICQ
                     SAQGDKSGLWTTVLAAAVFITD"
     gene            7433..8488
                     /gene="carA"
                     /locus_tag="PK154_00820"
     CDS             7433..8488
                     /gene="carA"
                     /locus_tag="PK154_00820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPW09636.1"
                     /translation="MKAVLGLEDGTFVLGQGFGTEGECAGELVFSTQMSGYMEALTDP
                     SYHGQILMFTFPTIGNYGVDKRNFQHPKVWAMGCVTREICDAPEAYPRIADYFADRGL
                     LGIQGVDTRMLTIKTREKGTLRAALVVGDDNGEYAVSCARDVPPISERALIPAVSCRE
                     PYHIPGPGKRIAVLDLGIKKNMVLSLLARKADLHIFPHDTPSDQVMACRPDALFISNG
                     PGDPVHATGAIRCISDLAGTLPIFGICMGIQVCGLALGGSTYKMKFGHRGSNQPVRYK
                     DGSIYITTQNHGFAVDADSLPEGCRVLYSNVNDGSLEGFENGYLDITCVQFHPEAHGG
                     PRDTEIPFFDTMFRRLE"
     gene            8488..11649
                     /gene="carB"
                     /locus_tag="PK154_00825"
     CDS             8488..11649
                     /gene="carB"
                     /locus_tag="PK154_00825"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPW09637.1"
                     /translation="MPKKPEIKKVLLIGSGPIQIGQAAEFDFSGTQACRALREEGVKV
                     VLVNSNPATIQTDPETADVIYVEPIRAEIIEKIIEKERPDGILSGMGGQTGLNMTAEL
                     AERGALANVQVLGTPLEAIYQGEDREKFRSLMEEIGEPVPKSMILNSLSQVDEALAIV
                     GLPAIIRPAYTLGGSGGGIAHTRDELIRIIEIGLTRSRIHQVLVEESVVGWKEIEFEV
                     MRDAADTCIIVCGMENVDAMGIHTGESVVVAPILTLRDDEFQLLRSASIKIIRALDVQ
                     GGCNIQFAYKDGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLHLDEITNSV
                     TGCTPASFEPAIDYIVVKVPRWPFDKFKNADRTLTTAMKSTGEVMAIGRCVEEAFKKA
                     LRSLDTDVYKHTNANEIRMILSRPTDERFPTLFDAFKAGFPLAEIQDLTDIAPFFLEK
                     IKNIVDLEQRLSDSATKGDIILARKYGFTEPELTALTGISPADIREIAGDPAYKMVDT
                     CAAEFPATTPYFYSTLDKECEIPPNDRNKVLILGSGPIRIGQGIEFDYCTVHAVKALR
                     EEQVEVHIVNNNPETVSTDFDTSDRLFFEPMALEDILNILEKDHYSGVMVQFGGQNAV
                     NLAVPIHEELIRRGMDTRILGTTPDSMDIAEDRDRFSELLRDLDIPSPPNSSAYSLGE
                     AREKAAAIGYPVLVRPSYVLGGRAMEIVHDEIELESYMKEAVRVSRNHPVLIDSFLQN
                     AIELDIDAVCDGDEVLIGGIMEHIEEAGVHSGDSACVIPTQSLSPAVLEKVRDYTTRI
                     ALGIGVVGLVNLQMAVKGETVYILEANPRASRTVPFVSKATGIPLAKIAAKVMIGKKL
                     KDLGIGEPHICHTAVKEVLLPFNKLPGVDTVLGPEMKSTGEVMGIDYDFGRAYYKACI
                     SADNELPVRGNIFISVISEQKDEVIPIARKLRELGLALYGTSGTVDYLSDAGIDAHLI
                     RKVQEGSPNVIDLLRKGEIRLIINTPSDKQSRQDHYQIMRAAVDYGVPYITTVQAARA
                     AAYAIEAIKKERITIEPLAHYHQ"
     gene            complement(11952..12536)
                     /locus_tag="PK154_00830"
     CDS             complement(11952..12536)
                     /locus_tag="PK154_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPW09638.1"
                     /translation="MLSLSIGLFLAGFIINFLSAFVIIRLIYYPRGRCNNFAFTFLAF
                     NTVIYFIMGLFTSIELSIAAGFGLFALFSILRYRTETVPIREMTYLFVMAALPILNAV
                     LWGLSSYALQLVVDLLVIGTLWVLDKEWGFNRSQFQMTVRYEKIDLIKREKKDEMLAD
                     LRERTSLPVQDFEILSIDFLRDCAEIRITYSGEV"
     gene            complement(12530..13351)
                     /locus_tag="PK154_00835"
     CDS             complement(12530..13351)
                     /locus_tag="PK154_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPW09639.1"
                     /translation="MAIGQRETVMPYNPGEKPALCSVISECESISLSELEETKAQLLT
                     RVESKHLMTLGQCRELVRTLAGSYRILEIQGIKIGRYETMYYDTPSFLTYLEHHNRKG
                     NRFKLRLRHYDTSDETYLEVKKKTNKGSTEKSRIRTSWSTSGFSPDQVAFLRAAFPYD
                     CRAFHPVLWTVYNRCTLVSKKIPERITLDTGIMFGDGEKVISYPDLVIGEIKYEKGVK
                     NSPALLALHGMSIRKRAFSKYCIGVSLLYSWLKHNRFKENLLFLSKLSGGGTAAC"
     gene            complement(13354..15816)
                     /locus_tag="PK154_00840"
     CDS             complement(13354..15816)
                     /locus_tag="PK154_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPW09640.1"
                     /translation="MKSMTALILAFIIAVTFTGWVNAETAGTDSGVTSVTLKGSSITV
                     EGSGATVDGTTLTISSGGTYTISGTLKDGQIRVDAKDEEMVKLVLNGATIACSASAPI
                     YVVNAEKTVITLAEGTKNYVTDGDSFIAEDAESDEPDAALFTNDDLTINGDGSLTVSA
                     SYNHGISVKDDLRINGGQITVIAVNDGIKGRDSVTVRDGTITVKAGGDGIQSNNDEDA
                     EKGIISIEGGTIAVTAGEDGIQAETSLSISAGEITVTSGGGSGMGSTSDTWGPRGMEP
                     SETESDTGSAKGLKAGGALTVSGGTITLDSSDDSIHSNDSMTINGGTITVSSGDDGIH
                     SDSTLEINGGDLRITKSYEGMESAVITLNAGTMHIVASDDGINIISSDDASVVPGRPD
                     QNAFTATGANYLHINGGYIVIDANGDGIDVNGPIEMTGGTVIVNGPTNNGNGALDYLG
                     IFRMTGGYLLAAGSSGMAQAPGTSSTQYAVMLTFASAQPAGTLVHIEFDDGKDILTFM
                     PTKTYQSIVLCSPDLEKGGTYVVYTGGRSTGSVTDGLYSGGIYTPGTEITSFTIAGIV
                     TNAGASNNQNPGTNPGSGTNPMNPGTNPRGKPGTPGVPTIAHSGTGTLSVTSSPRGAS
                     VSLDGAYKGVTPVIITGVLSGTHQITVTSPGYQDYSTGVSVTAGQMASVVASLKDQIG
                     STSTSLTTPTTAPTKAPPGTETWTLSVTTTPGGATVSLDGTYRGLSPLTLTGVSDGSH
                     ELKISKTGYQDFHTSVMVTGGKTTTVSAPLTSSDIGLSQPASSFRTNHPVNTLFPRMI
                     SASSVLIEVGSALLRSLYHGFG"
     gene            complement(15959..16207)
                     /locus_tag="PK154_00845"
     CDS             complement(15959..16207)
                     /locus_tag="PK154_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09641.1"
                     /translation="MYLIGSTSWIIILPEPTPGKRREQSGINEVGGMEEVLSSFLFSW
                     QIPFFVPASWSQRVTTRVLRRIRLSPLRTNIEKSIYIM"
     gene            16234..17421
                     /locus_tag="PK154_00850"
     CDS             16234..17421
                     /locus_tag="PK154_00850"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW09642.1"
                     /translation="MGKGTVVMAYSGGLDTSICIPLLKERYDYDRVVTVAVNVGQGDE
                     EIAVATKKGEKFADAHYTIDSRDRFVTEYLFPAIRANGSYEGYPLGTALARPLIAEEV
                     VRVALREKARTVAHGCTGKGNDQLRFDFIFRIAGLEVVAPIRELNLTREWEIEYAKEH
                     KIPVPVMKEKPWSIDENLWSRSIEGGRLEDPAYHPPDEIFGWTIPVEKAPDKPEQITL
                     EFLKGIPIKMNGEAMSGRDLITKLNIVAGKHGIGRSDMIEDRILGLKARENYEHPAAT
                     VILAAHADLEQLTLTRQELAFKRIVDDKWSELAYMGLVHEPLFHDLNAFITESQQKVN
                     GKVDLQLYKGCCRVLGRLSPEGIYSDDLVSFDSTTIDQCHATGVSAYYGIQARLVEER
                     KKK"
     gene            17602..19362
                     /locus_tag="PK154_00855"
     CDS             17602..19362
                     /locus_tag="PK154_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09643.1"
                     /translation="MKILWKYLKPQKWLILVALILAAISQLLNLIDPVIFGKIIDDYA
                     LNPANLPEAELVSSVLFWLGIAIAIALLARLARAFQDYLVRLVVQKFGMQIFNDGLKQ
                     TLRLSYSEFEETRSGETLSVLQKVRTDTQIFMNTAINVLFSSLIGMGFLIGYAVTKSW
                     LLIPVFLIGIVVLGSLTGLLSKKIKTTQREINRETNKMSGVITESLRNIELVKSLGLT
                     FPEIRRLREHNLKIFTLEMTKVKKVRTLTFLQGTTLSILKSSILFILLWLIFRNVLTT
                     GELISMQFISTTIFGPLQELGSFILSYREVEASVNTFDQLMHKPIEQNPENPIIVEDL
                     ESIRFADVVFRHRTASCNAVDGITFEVKRGETIAFVGPSGSGKSTLVKLLVGLYRPVS
                     GEIYFNGHPSSQIRYNPLRRQIGFVAQEAQLFAGTIRENLLFVNSDATDQQIRDALHK
                     AACDDLVARSSKGIDTIIGEGGMKLSGGEKQRISIARAILRRPRLLIFDEATSSLDSL
                     TEQDITNTIRDISLSREQISILIAHRLSTIMHADVVYVLEKGKIVETGSHDALLAQKG
                     LYYAMWRQQIGERRKGVNRT"
     gene            complement(19637..20326)
                     /locus_tag="PK154_00860"
     CDS             complement(19637..20326)
                     /locus_tag="PK154_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09644.1"
                     /translation="MTILSEIVEAVTSSMRDLIVPMIAYIPYLIAAIIILIIGWIIGR
                     FLGGIISKILDKAGVDDALRKTSIGKAIEQSGTNIVHLFDLIVRWFIYLIAIAAAASV
                     LKIGFISDLFEDFILYLPHIAMFLIILIAGFILIDYLLDLIQAWGKTQDIEFLGVILV
                     ALRIFFYFVIVILALSQLLIDLTIIYTFVTPIAWGVGIGIGVGIAVILAFGLKDRAPE
                     MVSNLLNKIHK"
     gene            20815..21039
                     /locus_tag="PK154_00865"
     CDS             20815..21039
                     /locus_tag="PK154_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09645.1"
                     /translation="MHWQDVVYRGFIAGSALLFIFLMFGIIRDYTTLAQEPTGSTACC
                     VNAATFTVAIACGVVLLSMGMFLIRKHMRV"
     gene            21384..21758
                     /locus_tag="PK154_00870"
     CDS             21384..21758
                     /locus_tag="PK154_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09646.1"
                     /translation="MKKTTLFLIVLLVFSTMLIAGCTQQTPEMKPVTTPTVTEKPTLP
                     PQVNQTVANITEKVNQTAANITEKANQTVANITEKVNQSATNLTEKANQTVANITSQA
                     NQTAANLTSQTNKTVADIVNKT"
     gene            complement(21950..22954)
                     /gene="ligD"
                     /locus_tag="PK154_00875"
     CDS             complement(21950..22954)
                     /gene="ligD"
                     /locus_tag="PK154_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPW09647.1"
                     /translation="MHPLLAALTEEQQKRLVAREQPAWIEPMLATLASPGSMSGDWIF
                     EQKFDGERCLVFRDRHRIRLLSRNRNVLNDTYPELVEALEAITDGQFIADGEIVALEG
                     ETPIFSRLQERMQIHDPEKARRSRIPVVLYLFDLVYLDGFDLSSLDLRTRKTLLASLI
                     PFRDPVRYSEHQEDGLEFYRNACRRGWEGIIAKRASGRYLHRRSSDWRKFKCLNRQEF
                     VIGGYTDPEGSRPGFGALLLGYYQDGRLLSAGKVGTGFDDRTLREIGARLVSLEQQAS
                     PFADKERPEKGEHFVTPVLVCEVIFTEWTPEGKLRHPRYLGLRDDKPAREVVKEKPQS
                     "
     gene            complement(23153..23542)
                     /locus_tag="PK154_00880"
     CDS             complement(23153..23542)
                     /locus_tag="PK154_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW09648.1"
                     /translation="MTSTGTPGQLPTLLLAHEKARARAWKEKNMRALDILLAPDFIEI
                     NALGRFSKKEILATLLPSLTLHEFIISDPRMVVASPDAAVLIYQCIEDITIRGQRISG
                     TFHVAAHYAKRENKWVLLVWQITPVSC"
     gene            23696..25777
                     /locus_tag="PK154_00885"
     CDS             23696..25777
                     /locus_tag="PK154_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW09649.1"
                     /translation="MSGSRKRREEDRSDSAAGATGSPPAGDLKAENEALKREITALKA
                     SLAAPEGYEAVLKESDEKFRNLLQGIYEGIILNDDKGVILEWNTGMEEIIGISRDDAI
                     GRTIFEVASRCVPESNDAGNGKKWLSAIIDTLDRTPTSRSHSPHLEIAVKHPDGALRT
                     IEVKNFYYTVLGRTFYGGIVRDITDLKMILENLSEREDELNNLIQSSSDAILLTDEEG
                     RITKFNKAQERLTGLAADEVAGLPAWEFQALRSSPKGPGLDPLTWYKEIWDRLLHDEN
                     DQFYREIVEDDITSTTGAVHHVQVRAFRIPTKKGFRVGAIVRDITDLKRTEVALLESE
                     EKYRTLIEMSPDAIVIHDGGIILYANPATAHLFHASHPENIIGRDAFTIIHPDSQAIS
                     RTVAEKDKEGISTLPTEIQVIRKDGTIMTVEGRGRRIQYQGKPAIEVILRDVTERKKS
                     EQQLREYAANLKRSNEDLELFAYIATHDLQEPIRGIVAYSQLLLAECRSDHSSQTKKY
                     LENIARDGLRLNTLVADLREYLRVRSGGRPFAPTDMERILQDTLHTLHRQVTETQATI
                     THDPLPIVQADSSQMTQVLQNLLANAMKFQQKGIAPTVHVSASSLDGMWQFSVRDNGV
                     GIPEGYFEKIFILFERLYPRDTYPGTGLGLALCKRIVERHGGRMWVESDEGRGSTFYF
                     TLPVTAPESSV"
     gene            25913..>26266
                     /locus_tag="PK154_00890"
     CDS             25913..>26266
                     /locus_tag="PK154_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HPW09650.1"
                     /translation="MFGELLTLFASTEIFIFVFMEIVTKLETIQRVIFKKATVRDYAV
                     FVVIFGLFSIFGTYIGIPWEHGAISNIRDLAPIVAGFVAGPYIGVAVGLIGGIHRLFL
                     GGTRQYRAVLRRSLQD"
BASE COUNT         5824 a         7281 c         7439 g         5722 t
ORIGIN      
        1 gagcctccgc ttgacgattc acccggattt cctgattctg acttccttct tgttgccgat
       61 cactgatcgt attcttattc ggggtatcgg agctgctcaa gagatagacc tgaaaaaacg
      121 gcgaccagaa accaccattc cggcctgaat ccagttctgg ctgaggatga gcgaggtctc
      181 gtcccggcgt tatgctgcac cctcactttc accctgcccg gggcacagcc tgtagaggag
      241 gtgaggagat tgattgacca tgagcggtga tccgcctctc ctccgatgcc ccagctgtgg
      301 ttcgctgctg acgtccgcga tcggagatac gggcatgtgg gaatgccact accatctctg
      361 ccatgcggta ttcccggcat tcatttccgg cgaggcgttt caggacgccc aggtcgtggc
      421 agaagctact cctccctatc tgccaggaga ccagcaacac agatcgaaat ctctttgata
      481 attgtcagac aactctgttc tgttctgcaa ggacaccatg cagccttctg gtgcaccaca
      541 ggattcctgg ttccagggat cttcacctgg ttggttgatc ggtgcagaac aaccctccgt
      601 gcgagtgcaa gaactcgcgc caccttgaat gcctggatca cctcggtcgc ctggtagggc
      661 cggggtgacg atggcgtttc tgttctttgc ggggatgact ccctgtctta ttattcataa
      721 aaagcatctt tcggtgcagg atgaacccca cgtcccgaat ttcgtggtca gatgcatgga
      781 tatggctttc cgcctgttat cctaactccc cacatacagt taggagtaaa cagcccccgg
      841 ggatggtgaa cttatccgtt cagatagttc cttttcagat ccgcgaaaag gcgttccacc
      901 tgctgtactg ctgtccccgt gtagcaggaa ggattgagca gcccccggga tggtgccctt
      961 atccgttcag atagttcctt ttcagatccg cgaaaaagtc gttccacctg ctgtactgct
     1021 gtccccgtgt agcgatcagg attgagcagc ccccgggatg gtgcccttat ccgttcagat
     1081 agttcctttt cagattcgtg taaaggcgtt ccacctgctg tactgctgtc ccagtgtagc
     1141 gatcaggatt gagcagatcg tggatctcct ggcgggagag ccaccgggcg gtagcaggat
     1201 cctgcgccag tacctcatca agtggcttcc cctcggcgag ggccttcatg ctcgcgacgc
     1261 ggaccatctc gtgggcttgc tgacggtcca tccctttccc ggcaagtgcg atcatcacgg
     1321 actcggccat gttcaccccg tggaggaggt cgaggttctt ccggatgttc tcctgccgga
     1381 tgaccagccc ttccagcacc ctggtaagaa gactgatgca gtggtcggca aggatggtcg
     1441 cctcgggaaa gatcacccgc tcgcaggagg agttcgtgag gtcgcgctcg tcccagaggg
     1501 tgttgttctg gagggccggc tcgactgccg accggatcac ccgcgccagc ccgcagacct
     1561 gctcgctcct gatgggattc ctcttgtggg gcatcgtgct cgacccgacc tgcttcttcc
     1621 cgaacgcctc ctcaacctcg gcgatctcgc tccgttgcag ggtccggagc gctatcccga
     1681 tcttgtcaag ggtcgttgcc acgttggcaa ggaacatgaa gtactcggcg taccggtcgc
     1741 gtgcgatcac ctggtttgac accgccaccg ggacgagccc gaggagttcc atcatccggt
     1801 cctggatctc catccccttc tccccgagag ccgcctgcgt tcccaccgca ccggtgagct
     1861 gaccgaccgc gacccggggg gtgagctcgg acaaccggtc caggtgccgc gctgtctcag
     1921 ccgcccagat ggcgaaccgg aggccatagg tcgtcggcac cccgatttgg ccatgcgtgc
     1981 gggcagcaca aacgaggtgc cgcgtgtcat ggctccgttt cagcaggatg gaaaggaggt
     2041 tccggagctt tgcatcgagg agcccgagcg attccttcag ctggagcccg gttgcagtat
     2101 cgaggatgtc gtttgaggtc gcgccatagt gcacccatcc cccggattcc ccgcacacct
     2161 cggcgatcgc tttcacgatt gccatcatgt catggttgat ctcggcctcg atctccttgg
     2221 cacgggagag ggatgcccgt ttcgcgttct ctccgatacg atcggcagca gcctccggga
     2281 ccagcccgaa gtcggcccct gccctggcga gagctgcctc cgcccggacg atgcaggcaa
     2341 accggttctc ctcggtccag accgcacgca tctcgggagt tccgtaccgg taatcgatgg
     2401 gatggatcgc catatgctga aagattctcc tttggaagaa taaaaaacag tccgggacaa
     2461 agagtaggtg atggagaatc cggacgccta tttcccgtac gagtcgtatc gcccgcacca
     2521 gaaggaaatg ctggcaagcg tggcagcatg tgcacggcaa ggcgggattg ccatgatcga
     2581 tgccccgacc gggagtggaa aatcgagcgt ggtcgcagca ctcctcgcgg agcgctccgg
     2641 gaggaagatc tttgtcgcgg tccggacgat cagccagctg aacacgttta tcagggaact
     2701 cgaactgatc aggaagaaga aacggccgat ctcctactcc tatctgatcg ggaagcggag
     2761 cctttgcatg cttcccgggc agggggacat ctaccggaga tgcgaagggg tcaaggccgt
     2821 gtcaaccagc ctgatgcggg agagggcaga aaagggatcg ctcgtcccct ctaaagatcc
     2881 cgtcatcacc cagcagatcc gaaagctcga tgaggaacac ccggtcatct gcccctacta
     2941 tatccggagc agggtgtttt tgaccggcga ggggagcggt ctccggatgg tcccctccac
     3001 gccgctgaaa gccaaggcag agcgggtctc gcgggagctt gcccgcccgg gcgagctccg
     3061 ggatatcggt gccgggatct gtccctatga aatgctcctc ctcgcagccc agcggtctga
     3121 tgtcatcgtc gtgaattatc accacatctt cgatgaggat atcaggaatc agctgtatgg
     3181 atcacttggg atcgaaccgg ctgatgcgat gctcctgatc gatgaagccc acaactgcgg
     3241 cgaggtgatg accgggatca tgaacgtcgt ggtcggtgag caggtccttg cccaggccga
     3301 ccgggagatt gctgcgatcc gccgccatct gaagagcaca gcagccatcg ggcagatcat
     3361 ttcgaatatc gccctcttca tgcaggggct gaagaactcg cacgagacgg aggactggtt
     3421 tgatcctgcc atctttgacc ggatggtggt ccggggctct ctctacaagg atatggccga
     3481 ggtggttgaa gacctgatga agatcagcga atacatcagg gagaagaaca tgatggccgg
     3541 cgagtactcc atgagtgcca ctgagatgct caccgcattc ctcgtcagga tctcctgctc
     3601 atcaaccgat ccgggatacc tgacagtgta ccggaaagag gagccggata tcttcctcga
     3661 ggtgaggagc atcgacccct cagaccggct ccgtgacctc gccctgtccc accactgcac
     3721 cgtcctcata tcgggaacgc tggcgccgct cgagtcctat cagcggtact ttttcggcga
     3781 cctgccggtg accggctgct cgcttcccaa cacgttcccg aagaagaacc ggatggtcct
     3841 ctgcgcaggg gacatcacga ccgcgttctc gatgcgcgag aaccgggaga actcggcccg
     3901 ggtagcccag tatatccagc attttgcgcg gctgaagggg aatatcgcgg tctacttccc
     3961 ctcctaccag atcctcgaca cctatgcccg gctgctcgcc gctccccttg cctcccggaa
     4021 cctgatcatc gagccgaagg atgcgagaga tgccggtgag gcgcttacga ccttcctctc
     4081 gctcccctcc aaggggaagt ccgggatcct ctttgcggtt gccggcggaa aatggagcga
     4141 ggggctcgat taccgaggag aactcctctc aggtgccatg gtgatcggcc tccccctcgc
     4201 cccctttaac caggtacgaa agatgacgat cgattatttc cggcacaagt tcgggccgga
     4261 cggcgagttc atctcatata cccttcctgc cctgaaccgt gccgagcagg cgatagggag
     4321 ggtactccgg actcccgaag accggggtat ccttgtgctc ggtgagaagc ggttcctgga
     4381 accacgggtg aaggccggcc tcccgggctg gatccgcgag gagatggtgg aatgcaccgc
     4441 agaatcattc ggcgaggtga tgaagcggtg gaagtgatcg agggatcagc ccgattcggc
     4501 ctctggtatg tccacgtccg ctggtcagga actaccgtgt accaggtccg gttcagccgc
     4561 acccccgtac gtggaccagt ccctgtcccc atcacccagt acctctccgg caggatcgtt
     4621 gacctttcca cgctcacctg cgggtctccc ccggccgggg aacactatgc ccggatctac
     4681 ggggaagtac agcggatccc atacggagag accgcgacct atggagaggt tgcagaatgg
     4741 tcggggacga ccgcgagggt ggtcgggcag gccctctcgc ggaatatcct cccgctgatc
     4801 atcccctgcc accgggtggt agcgcgggac ggaatcgggg gatttacccc tgatcccgcg
     4861 ataaaagtgg atcttctggc gatggaagag aggagcaaac ggcaaagtag gagaaggact
     4921 gaggactgat gatgatgaaa gggcctggat caggcaccat gagatctggt gcccgcaaca
     4981 ggattgcagg gaagggatcc ggagaagaga agtgctccgg gctgcacggg aaggccatgg
     5041 cgtgaaaccg cagggagaaa aaaagaggcg tggtatgagg tcagcaatcg tccttgtcgg
     5101 aggggaagcc aggcgggtga acggcatgga gaagtatttc ttcaggtatc tcggaaagac
     5161 cttcatcgaa cggctgatcg atgccctccg gcctgcagtg gacgagatca tccttgtggc
     5221 aaaggacgat gagcactgtg aacggttcgc ccatatctac gaagcccggt gtgtccacga
     5281 catcaggaaa ggcatggggc cgataggagg gctccatgcc ggggttgcgg catgcaaggg
     5341 cgatcccatc ttcgtctgcg cctgcgatat gccctgcatc gacaccatgg tcgtctcagc
     5401 cctctttgat gcattagggg actacgatgc cgcgatcccg tcctgggaca cggagatgct
     5461 cgaaccgctc catgccgtgt accggaaaga tccccttcaa cactatcttg aagatccccc
     5521 ttccctctcg ctccgttccc tggtaaagca cctcaatgtc acctttgtcc cggtgggccg
     5581 gatccaggcc atcgatccgg acctttccac gttcaccaac atcaacaaac tcgaggatct
     5641 ggagtatctc ggccgcgaag attccgattg accggttcca tgcccggctg atatcatatc
     5701 ctgaagagaa gccagtgggg attctgccgt gaaaagaagg gaggagaggc agagttcaga
     5761 acgaacaggc catctttttc aggggcgatc ccgcaggatt gccttctttt ggcaccctga
     5821 tctggacctg tgcggagagt tcattcccgg aaaattcgat caaatacttt ccctgggacc
     5881 ggatggtgag atcttttccg gtatcggtac tgtatccccg tccaaatcct tcctttgcga
     5941 cgatctgccc tgaagcaggc tcgcggacgg tcacctcaaa ccatgcagcg gggctgatgg
     6001 tggtgacggt ctctgtcttc tctccccggt cagtgtattt actgtcatac cagatcgtcc
     6061 gggtggtcat attcggtgag aaacagatct cgatcacgag gggaggcttc tggaggtcgt
     6121 atgtgaaggc ggtccggttg ttcttcagtg cgagggtgct atagtaaata cccatgtatt
     6181 ctaccgggga gggagtaatg tcggggaggc gggtgcctga catggtcgat gccgggatgg
     6241 cctctgtcgg gtatggtgtg gcagggacca gatacccggc ttcaccggga gccggggttg
     6301 gtgtggtggt cgttgtgctc cctccgtctg tcccggcagc agtcggggtc gcctttgctc
     6361 cgctgatacc cccttttgaa gcggcagtgc ccccttgagc tgctgaaccg gtattcttct
     6421 caggggggga gatgcatccg gaaaggcata ccatggccac aatgacgagt atgcagataa
     6481 tgcctgtcct cattcccgtc ataaagggag ggttctgctc gagcccatat attgattatt
     6541 ccggataatg ttaatggaga ggatgcattc agacaggcca gaatgccgcc aatccgcaga
     6601 taaaagagag ggcgtctcag tccgtgatga acacagccgc cgcaaggacg gtggtccaga
     6661 gccctgattt gtcaccctgg gcagactggc agatattcgt ggtcttgatg atcttgccgc
     6721 ttgccttgta gatctgttcc cgttcctgcc aggcctggtt gatatcaaac tcgatcccga
     6781 gggtagttgc aagcatggtg gctgcaaggt cctctgcata gtccccggtc ttctccgctg
     6841 tttcgccgta tgcgtggtgc tctgaaaggt acccgtactc ctgtgagtcc ttcgggaggg
     6901 cgagcccaat cgccgcagag atcaggcggt tcggttcgtt ggtctcgttc ttggccatga
     6961 cacagtaggt gatgctgccc ggctcaaggc accgtgcccc ttcctcaacc gtcaccagtc
     7021 tgcagaaggg aggaaagatg ctcgatacat agacaaggtt gtacttctcg atccccgccc
     7081 tccgcagtgc aagctcgaac gatgcgagac gatctttatg tacaccaaca cccttggtga
     7141 aaaatatctt ggagggtacg gccatgatca taactgagga ttaggcaaac ttaaaattta
     7201 ttattcagaa aacgaacact taaaaagcgt tcattccgat atcgaaatgg tttaaccctg
     7261 ttaaataaag aaaaaacagt caattattct tatatcgtga aaagaagaga ttataaaaat
     7321 aattgaggga gtgctgcggc acgcggattt gatcgcattt tcctctccag gaccggtgtt
     7381 ttctccgttc cgctacctat taaatgtaat cctgcgcatt acgtatggaa cgatgaaggc
     7441 ggttctgggt ctagaggatg gcacatttgt gcttgggcag ggttttggaa cggaagggga
     7501 atgtgcgggt gaactggtct tctccaccca gatgtcggga tacatggagg ctctgactga
     7561 cccgagctat catgggcaga tcctgatgtt cacctttccc accattggca attacggggt
     7621 tgacaagagg aacttccagc acccgaaggt gtgggcgatg ggatgtgtga cccgggagat
     7681 ctgcgatgca cccgaggcat atccccgcat tgccgattat tttgctgacc gggggctcct
     7741 cggcatccag ggagtcgata cccggatgct caccatcaag acgagggaga agggaaccct
     7801 gcgggcggcc cttgttgtcg gggatgacaa cggggagtat gcggtcagct gcgcacgaga
     7861 cgtccccccg atctcggaac gagcgctcat ccctgccgtc tcctgccggg agccatacca
     7921 tatcccgggg ccaggcaagc gtatcgcggt gctcgacctt ggcatcaaga agaacatggt
     7981 cctctctctc ctggcacgga aagcggacct ccatatcttt ccccacgata ccccttcaga
     8041 ccaggtcatg gcatgccgcc ccgatgccct cttcatcagc aatggccccg gggacccggt
     8101 ccatgccacg ggagcgatcc gctgcatcag cgatcttgcc ggcacgctcc cgatcttcgg
     8161 gatctgcatg gggatccagg tatgcgggct tgccctcggg gggagcacct acaagatgaa
     8221 gttcggacac cgcgggagca accagccggt gcgatacaag gacgggagta tctatatcac
     8281 gacccagaac catggatttg ccgtcgatgc cgattccctc cccgaaggat gccgggtgct
     8341 gtactcgaac gtgaacgatg gctctctcga aggtttcgag aacgggtatc tcgatatcac
     8401 ctgtgtgcag tttcatccgg aagcccatgg cggaccgagg gacaccgaga tccccttctt
     8461 tgatacgatg ttccggaggc tcgaataatg ccgaagaagc cggagatcaa aaaagtcctc
     8521 ctgatcggat ccgggccgat ccagatcggg caggcggctg agttcgattt ctcgggcacg
     8581 caggcatgcc gggcactccg cgaggaaggg gtgaaggtgg tccttgtcaa ctcgaacccg
     8641 gcaaccatcc agaccgatcc cgagacggcc gatgtgatct atgtcgagcc gatcagggca
     8701 gagatcatcg agaagatcat tgagaaggag cggccggatg ggatcctttc cgggatgggg
     8761 ggccagaccg ggctgaacat gacagccgag cttgccgagc ggggggcgct tgcaaacgtg
     8821 caggtgctcg gcacccctct cgaggcgatc taccaggggg aggaccggga gaagttcagg
     8881 tccctgatgg aggagatcgg cgagccggtg ccaaagagca tgattttaaa ctcgctctcc
     8941 caggtggacg aggcactcgc cattgtaggg ctccctgcca tcatccggcc tgcctacacc
     9001 ctcggggggt cagggggggg aatagcccat acccgggacg agctgatccg gatcatcgag
     9061 atagggctga cccgctcccg catacaccag gtgctcgtag aggagagcgt ggtcgggtgg
     9121 aaggagatcg agttcgaggt gatgagggat gctgctgata cctgcatcat cgtctgcgga
     9181 atggagaatg tggatgccat ggggatccac acgggtgaga gtgtcgtggt cgccccgatc
     9241 ctgacgctcc gtgacgatga gttccagctc ctcaggagcg cctcgatcaa gatcatccgg
     9301 gcgctggacg tccagggagg gtgcaacatc cagttcgcgt acaaggacgg agattaccgg
     9361 gtgatcgagg tgaacccacg ggtctcccgg tcgagtgcgc tggcctccaa ggcgaccgga
     9421 tacccgatcg ccagggtcgc ggcaaagatc gcgatcgggc tccacctgga tgagatcacg
     9481 aactcggtga ccggctgcac cccggcatct ttcgagccgg ccatcgatta tatcgtcgtc
     9541 aaggtccccc gctggccgtt cgacaagttc aagaacgccg accgcaccct caccaccgcg
     9601 atgaagagta cgggcgaggt gatggcgatt gggaggtgcg tggaggaggc gttcaaaaag
     9661 gcgctccggt cgctcgatac cgatgtgtat aaacacacga acgcaaacga gatccggatg
     9721 atcctttccc ggccgaccga tgagcggttc cctacgctct tcgatgcctt caaggccggg
     9781 ttcccgctcg ccgaaataca ggacctgacg gacatcgccc ccttcttcct cgaaaagatt
     9841 aagaatattg tcgatctcga gcagcggctc tccgattcgg caacaaaggg cgatatcatc
     9901 ctggcacgga agtacggctt caccgagccg gagcttaccg cactgacagg gatcagtccc
     9961 gctgatatcc gtgagattgc cggggacccg gcctacaaga tggtcgatac ctgcgccgca
    10021 gagttcccgg cgacgacccc ttacttctac tccacccttg acaaagagtg tgagatcccg
    10081 ccaaacgacc ggaacaaggt gctgatcctc gggtcaggcc cgatccggat cgggcagggg
    10141 atcgagttcg actactgcac agtccatgcg gtcaaagcgc tccgggagga acaggtcgag
    10201 gtgcacatcg tcaacaacaa ccccgagacg gtctcgaccg acttcgacac ctcggaccgg
    10261 ctcttcttcg agccgatggc gctcgaggac atcctgaaca tccttgaaaa agaccattac
    10321 tcaggcgtaa tggtccagtt cggcggccag aatgccgtga atctcgccgt acccatccac
    10381 gaggagctga tccggcgggg catggacacc cgcatccttg ggacgacccc ggactcgatg
    10441 gatattgctg aagaccggga ccggttctca gagctgctcc gcgatctcga tatcccgagc
    10501 ccccccaaca gctccgccta ttcactcggg gaggctcgtg agaaggctgc cgctatcggt
    10561 tacccggtcc ttgtccgccc ctcctacgtc ctcggcggga gagcgatgga gatcgtccac
    10621 gacgagatcg agctcgagtc ctacatgaag gaggcggtca gggtgagccg gaaccacccg
    10681 gtgctgatcg attcgttcct ccagaacgcg atcgagctcg acattgacgc ggtctgcgat
    10741 ggcgacgagg tcctgatcgg tgggatcatg gagcacatcg aagaagcggg ggtccattcc
    10801 ggggactcgg cctgcgttat ccccacgcag tcgctctccc cggcagtgct cgaaaaggtg
    10861 agggattaca cgacccggat cgcgctcggg atcggggtgg tagggctcgt caacctccag
    10921 atggcggtca agggagagac agtctacatc ctcgaggcaa acccacgggc gagccggaca
    10981 gtgccattcg tctcgaaggc caccgggatc ccgcttgcaa agatcgcagc gaaggtgatg
    11041 ataggcaaga agctgaagga cctcgggatt ggggagcccc atatctgcca caccgcagtt
    11101 aaggaggtgc tcctgccgtt caataagctc ccgggtgtcg acacggtgct tgggccggag
    11161 atgaagagca ccggcgaggt gatggggatc gactacgact ttggccgtgc ctattacaag
    11221 gcctgcatct cggccgacaa tgaactcccg gtccggggga acatcttcat atcggtcatc
    11281 tccgagcaga aggacgaggt tatcccgatc gcccggaagc tccgcgaact tggtctggcc
    11341 ctctatggga catcggggac ggtggactac ctctcggatg ccgggatcga tgcccacctg
    11401 atccggaagg tccaggaagg ttcgccgaac gtgatcgacc ttctccggaa aggggagatc
    11461 cggctgatca tcaacacacc ctctgacaag cagtcgcggc aggaccatta ccagatcatg
    11521 cgggctgccg ttgattacgg ggtcccgtat atcacgacag tccaggccgc acgggccgca
    11581 gcgtacgcaa tcgaggcgat caaaaaggag cggatcacga tcgagccact ggcacattat
    11641 caccagtaat caacggaacc cgattcactt ttcaattctg ctttttacga aagtagtacc
    11701 gaagtatcat cctgcaggca tgtttctcaa tggtgaatca ggtatttttt ccatcaccag
    11761 tgcctccggc acttcatcca ttactctcga ccgaacggta gtcaaaggag ctcctgtgaa
    11821 aattcctcca gtattatttc tcatctatgc atctgtctaa aaggtgaacc aggtgatttt
    11881 ttacgtatct tacatgaaaa ttctggagag gtaggaggta ttgtaattgc gatctccgaa
    11941 aaaatgagac cttatacttc ccctgaatag gtgatcctga tctctgcaca gtcccggagg
    12001 aagtcgatgc tcaggatctc gaaatcctgg acgggaaggc tggtccgttc gcggagatca
    12061 gcgagcatct cgtccttctt ctcccgcttt atcaggtcga tcttctcata ccgcacggtc
    12121 atctggaact gtgaacggtt gaatccccat tccttgtcaa ggacccagag ggttccgata
    12181 acgagaagat ccactaccag ttgcagggca taggaggaca atccccagag tacagcgttc
    12241 aggatcggca gggctgccat cacgaacagg taggtcatct cccggatcgg gacggtctcg
    12301 gtccggtacc ggaggatcga gaacaaggca aacaggccaa accccgccgc aattgacaat
    12361 tcgatgctcg tgaacaaccc catgatgaaa tagatcacgg tattgaaggc aaggaacgta
    12421 aaggcaaaat tgttgcaccg ccctctcggg tagtagatca gcctgatgat aacgaacgcc
    12481 gacaggaagt tgatgataaa tccggcaagg aacaacccga tcgacaagct cagcatgctg
    12541 ccgtacctcc gccggataat tttgagagga agagcaggtt ctccttgaac cggttatgct
    12601 tcagccagga atacaggagc gacactccga tacagtactt ggaaaacgcc ctcttcctga
    12661 tgctcatccc atgaagcgcc aggagtgccg gggagttctt cactcccttt tcgtacttga
    12721 tctccccgat cacgaggtca gggtacgaga tcaccttctc cccgtctcca aacattattc
    12781 cggtatccag ggtgattcgt tcggggattt ttttggagac gagggtacac ctgttataca
    12841 ctgtccagag aacgggatga aatgcccggc aatcgtatgg aaatgctgcc ctaaggaacg
    12901 ccacctggtc gggtgagaat ccggaagtag accaggatgt cctgatacgg ctcttttcgg
    12961 tggatccctt gttggtcttc ttttttactt cgaggtaggt ctcatccgac gtatcgtaat
    13021 gccggagcct cagcttgaac cggttgcctt tcctgttatg atgctccagg taggtaagaa
    13081 acgagggcgt atcatagtac atagtctcat atctcccgat cttgataccc tgtatctcga
    13141 gtatcctgta ggagccagcc agtgtccgca cgagttccct gcactgccca agagtcatca
    13201 ggtgcttgct ctccacccgg gtcaggagct gggcttttgt ttcttccagc tcggagaggg
    13261 agatactctc acattcgctg atcaccgagc agagcgcagg tttttcacct ggattatacg
    13321 gcatgaccgt ctcccgctgt cctatcgcca taatcatccg aacccgtgat acaacgaccg
    13381 gagcagggcg ctgcccactt caatcagtac cgatgacgct gatatcatcc ggggaaacaa
    13441 ggtattgact gggtgatttg tccggaacga gctggcgggc tgtgataagc caatgtcact
    13501 actggtgagt ggtgctgata cggtagtggt cttccctcca gtcaccatca cgctggtgtg
    13561 gaagtcctgg taccctgttt tggagatctt cagctcgtgg gatccgtcag aaacaccagt
    13621 cagggtaaga ggggagagtc ccctgtaggt accgtcaagg gagaccgtgg cccctcccgg
    13681 ggtggtggtc accgaaaggg tccaggtctc cgtgccgggt ggtgcctttg tcggggcagt
    13741 agttggagtt gtcaaggaag tggaggtcga cccgatctga tccttcagtg acgcgaccac
    13801 acttgccatc tgaccggccg tgaccgatac cccggttgaa tagtcctggt atccggggct
    13861 ggtgacggtg atctggtgtg tcccactcaa gaccccggtg atgatgaccg gggtcacccc
    13921 tttatacgca ccgtcaaggg agacacttgc ccccctcggg gaagaagtca cagagagggt
    13981 ccccgtccct gaatgtgcaa tggtggggac cccgggggta cccggttttc cccgtggatt
    14041 cgtacctgga ttcatgggat tcgtgcctga acctggattg gtacctggat tctggttgtt
    14101 cgatgccccg gcgttcgtca cgatacctgc tatagtaaag ctggttattt ccgtaccggg
    14161 ggtatagatt cccccggagt agaggccgtc agtcaccgaa ccggttgacc tgcctccggt
    14221 atacacaacg taggtcccgc ccttctccag gtcgggtgaa cagagtacga ttgactggta
    14281 agtcttcgtc ggcatgaagg tgaggatgtc cttgccatcg tcgaattcga tatgcaccaa
    14341 cgtgcctgcc ggctgggctg atgcaaatgt cagcattacc gcatactggg tcgatgaggt
    14401 gccaggggcc tgtgccatac ctgaactgcc tgctgcgagg agataccccc ctgtcatcct
    14461 gaagatgccc aggtaatcca gtgcaccatt gccgttgttt gtcggaccgt tgacgatcac
    14521 ggtgccaccg gtcatctcga tcggcccatt gacatcaatc ccgtctccat tggcatcgat
    14581 cacgatatac ccgccgttga tatgcaggta attggcaccg gtagcagtga aggcattctg
    14641 gtcaggccga ccaggtacca cagaggcgtc atcgctgctg atgatgttta tcccgtcatc
    14701 gctggcaacg atatgcatcg ttccggcatt cagggtgatg acggcacttt ccataccctc
    14761 gtaggatttc gtaatgcgca ggtccccccc gttaatctcc agggtggaat ctgaatgtat
    14821 gccgtcatcc cctgaggaga ccgtgatagt gccgccgtta atagtcatac tgtcattcga
    14881 gtggatcgaa tcatcggatg agtcgagggt gatcgtgccg cctgataccg tgagggcacc
    14941 cccggctttc agccccttgg cgctcccggt atcactttct gtttcggatg gttccatgcc
    15001 ccgtggaccc cacgtatctg aggtactccc catgccgctg cctccccctg aggtgaccgt
    15061 gatttcaccg gcactgatac tcaggctggt ttccgcctgg ataccatctt ctccagcggt
    15121 aacagcgatt gttccaccct cgatggagat aatgcctttt tcggcatctt catcattgtt
    15181 ggactggatt ccgtccccgc ctgctttcac ggtgatggtc ccgtccctga cggtgacaga
    15241 gtctcttccc ttgataccat cattcaccgc aatcacggta atctgtcctc cgttaatcct
    15301 gaggtcgtcc ttgaccgaaa taccgtgatt atagcttgca gagacggtga gtgaaccatc
    15361 gccgttgatc gtgagatcgt cattagtgaa tagtgctgca tcgggttcat ccgattctgc
    15421 atcttcagcg ataaacgaat ctccatcagt gacataattt ttcgttcctt ctgcaagggt
    15481 gatcaccgtt ttctctgcgt tgacgacata gatcggagca ctcgcagaac aggcaatggt
    15541 tgctccgttc aggacaagct ttaccatttc ctcgtccttt gcatctaccc gtatctggcc
    15601 atccttcaaa gtgccgctga tggtgtaggt gccaccggat gatatggtga gggtggtgcc
    15661 atcaaccgtc gccccgcttc cctcaacagt gatcgaactg cctttcagag taacagaagt
    15721 aactccggaa tcagttccag ctgtctccgc attcacccag ccggtaaaag tgacggctat
    15781 gatgaatgcc aggatcaatg cagtcatgct tttcattgtc gttagtctcc tttagctcac
    15841 tccattcctc attttcagcc cggttcgggg gtgccagttt tccggcggac tcccccttcc
    15901 ctgaacgatc ggaatggtgc cgattgtcaa atttactgta gataatgtat agtatacatt
    15961 acattatgta aatacttttt tctatgttcg tcctcaatgg ggacaaacgg atcctcctga
    16021 gaacccttgt tgtcacccgc tggctccagg aggcgggaac aaaaaacggg atctgccaag
    16081 agaacaagaa ggaggacagg acctcctcca ttcctccgac ctcgttgatt ccagactgct
    16141 cccttctttt tccgggcgtg ggttctggta gtattattat ccacgacgtc gaaccgatca
    16201 ggtacatttg tgcttgcagc acggagcgat accatgggaa aaggaactgt tgtgatggcg
    16261 tattcgggag gccttgacac ctccatatgc atcccgctcc tcaaagagcg gtatgactac
    16321 gaccgggtag tgaccgtggc cgtcaatgtc ggccagggag acgaagagat cgccgtcgcg
    16381 acaaagaagg gggaaaagtt cgccgatgcc cactatacga tcgactcacg ggataggttc
    16441 gtcaccgagt atctcttccc ggctatccgg gcgaacgggt cctacgaggg atacccgttg
    16501 ggtactgcac tcgcgaggcc cctgatcgcc gaggaagtgg tccgggtcgc cctccgggag
    16561 aaggcaagga ctgtagccca cggatgcacc ggcaagggga acgaccagct ccggttcgac
    16621 ttcatcttcc ggatagccgg ccttgaggta gtcgccccga tccgtgagct gaacctcacc
    16681 cgcgaatggg agatcgagta cgcaaaggag cacaagatcc cggtcccggt catgaaggag
    16741 aagccctgga gtatcgacga gaacctctgg agcaggagca tcgaaggagg gcggctggag
    16801 gacccggcgt accacccgcc tgatgagatc ttcgggtgga ccatcccggt cgagaaggcc
    16861 ccggacaagc ccgagcagat cacccttgag ttcctgaagg ggatccctat caagatgaac
    16921 ggcgaagcca tgagtgggcg cgacctgatc acaaaactga acatcgttgc cggaaagcat
    16981 gggatcggcc gctccgacat gatcgaggac cggatcctcg ggctgaaggc acgggagaac
    17041 tacgagcacc cggctgccac cgtcatcctc gccgcccatg ccgacctcga gcagctcacc
    17101 ctcacgcggc aggagctggc gttcaagcgg atcgttgatg acaagtggtc cgagctcgcc
    17161 tacatgggcc tcgtccacga gccgctcttc catgacctga acgcgttcat caccgaatcg
    17221 cagcagaagg tgaacgggaa ggtggacctc cagctctaca aggggtgctg ccgggtgctc
    17281 ggccggttat cccccgaggg gatctactcc gatgatctgg tctcatttga cagcaccacg
    17341 atcgaccagt gccacgcgac cggggtctcg gcttattacg ggattcaggc ccggctggtc
    17401 gaggaacgga agaaaaaata aagggtaaaa gagctccgct catcaccctt ttcgatagaa
    17461 gacgctcatg tattttcata ctgcacactc aaccgccagg tggtttctcc tccaaaaaga
    17521 ccacctctcc ttcctaaaga tttacctcaa ctcacgctac aggtacattc aggaggtcct
    17581 tccaaggaca cctccccacc gatgaagatc ctctggaagt acctgaaacc gcagaagtgg
    17641 ctgatccttg ttgccctgat cctggcagcg atcagccagt tgctgaacct gatcgacccg
    17701 gtcatcttcg ggaagattat cgatgattat gcactgaatc ccgcgaatct gccggaggct
    17761 gaactggtat cctcggtcct cttctggctc gggatagcca ttgcgatcgc cctgcttgcc
    17821 aggctcgcac gggcgttcca ggactatctc gtgaggctgg tcgtccagaa gttcggcatg
    17881 cagatattca acgacggcct gaagcagacg ctccggctct cctacagtga attcgaggag
    17941 acacggagcg gggagaccct gtcggtgctg cagaaggtgc ggacggatac ccagatcttc
    18001 atgaatactg ccatcaatgt cctcttctcc tccctcatcg ggatgggatt cctgatcggg
    18061 tatgcggtga caaagagctg gctgctgata ccggtgttcc tcatcggcat tgtcgtgctg
    18121 ggatcgctca ccgggcttct ctcgaagaag atcaagacta cgcagcgcga gatcaaccgg
    18181 gagacaaaca agatgtccgg ggtgatcacc gagtccctgc ggaatatcga actcgtgaaa
    18241 agtctcgggc tgacctttcc ggagatccgg aggctgcggg agcataacct gaagatcttc
    18301 accctcgaga tgacgaaggt gaaaaaggtc cggacgctca ccttcctgca ggggacgacg
    18361 ctctccatct tgaagagttc gatcctcttc atcctgctct ggctgatatt ccggaatgtc
    18421 ctcaccaccg gcgagctgat ctcgatgcag ttcatctcca cgaccatctt cggtcccctc
    18481 caggagctgg gcagcttcat tctgagttac cgggaggtcg aagcgtccgt gaacacgttc
    18541 gatcagctga tgcacaaacc gatcgagcag aaccctgaga acccgatcat cgttgaagac
    18601 ctggaatcca tccgttttgc ggacgtggtg ttccggcaca ggaccgcctc gtgcaacgca
    18661 gttgacggga taacatttga agtgaagagg ggagagacga tcgcatttgt cggtccctcc
    18721 ggttcgggca aatcgacgct cgtgaagctc ctggtcgggt tgtaccggcc tgtcagcggt
    18781 gagatctatt tcaacggcca cccctcatcc cagatcaggt ataacccgct gcgccgccag
    18841 atcgggttcg ttgcgcaaga ggcgcagcta tttgccggaa ccatcaggga gaacctcctg
    18901 ttcgtcaatt ctgatgcaac ggaccagcag atccgggatg ccctccacaa ggctgcgtgt
    18961 gatgatcttg tcgcccgttc ctcgaagggg attgatacga tcatcgggga gggcggcatg
    19021 aaactctccg gcggcgagaa gcagcggatc tcaattgccc gggcgatcct gcgacgtccc
    19081 cggctgctga tctttgacga ggcaacctca tcccttgatt cactgacaga gcaggatatc
    19141 acgaatacca tccgtgatat ctcgctctcc cgtgagcaga tctccatcct gatcgcccac
    19201 cgcctgtcca cgatcatgca tgccgacgtg gtctacgtac ttgagaaggg gaagatcgtg
    19261 gagacgggtt cgcacgacgc acttcttgcc cagaaggggc tgtattacgc gatgtggcgc
    19321 cagcagatcg gggaacggag gaagggagtg aacagaacgt aacactctcc cgtaatcctg
    19381 ctctcctgtc aaccaacagg atcccaatga tgctctgcct tccggatggg ggtcatcagg
    19441 cattcatgaa aattttgata gtgtcgcaga gggtgctatg cagcccgata ccagtatgac
    19501 cgggccaaag gtgttgcatc aggaaagggc cccgatgggt accgcctcat gaaatattcc
    19561 acttccgaat agcgaccaaa acaaggtccc ctctgaaagg atggatacac cagacagagg
    19621 cagagatgca aaaaaattat ttgtgaatct tgttcagcag gttgctcacc atctcgggag
    19681 cacggtcctt caggccaaag gccaggataa cggcgatacc gactccgatg ccgatgccca
    19741 ctccccatgc tatcggagtg acgaaggtgt agatgatcgt cagatcgatc agcagctggg
    19801 agagggcgag gatgacaatg acgaagtaga agaatatccg cagagccacg aggatgacgc
    19861 cgaggaactc gatatcctgt gtcttgcccc atgcctggat gagatccagc aggtaatcga
    19921 tcagaatgaa tccggcaatc aggatgatca ggaacatcgc aatgtggggg aggtacaaga
    19981 tgaaatcctc gaacaggtcg ctgatgaatc cgatcttcag gacacttgcg gccgcagcga
    20041 ttgcaatgag gtagatgaac cacctgacaa tgagatcgaa gagatgcacg atgttcgtgc
    20101 cggattgttc tatcgctttt ccaatggaag tcttccggag ggcatcatcg acacctgctt
    20161 tatcgagtat ctttgagata attccgccaa ggaaccgccc gataatccat ccgatgatca
    20221 ggatgatgat cgcagcaatg aggtatggaa tgtaggcgat catcggaacg atcaggtctc
    20281 tcatcgaact agtgacggct tcaacaattt ctgaaagaat ggtcatggta accagaatgc
    20341 ttttttagag ggagtatgct taaaattatg gataaggtaa ggggaagcta ttaatattgg
    20401 taatataata gaaccatttt tattcaattc gtgagagaga ccatcttttt gcaggggtat
    20461 cagggtgatg agagggagtt ttcgatccgg tgcatcacaa tgccagataa aatcaggtca
    20521 catttttttt atgacaggtg ccgctaattg ccatctgaga gatccgtcca cggatgttcc
    20581 gggtccactg ccggaatggc gagcagagga cgaccccggt tccagccggt ttcgctcatc
    20641 gacggacacc agcatgtcac gtctcaagag atcgctgggg aatagaaacc ctgcctgcac
    20701 tgatcagggg agagatcctg ttccaggatt tttttggcca ccgacctcct gaacatcgca
    20761 tccggggatc cgaacgaaaa gaacaacacc tcgccccggc aatagccctt gataatgcac
    20821 tggcaggatg tcgtctacag gggatttatc gcgggatctg cgttgctctt catcttcctg
    20881 atgttcggta tcatcaggga ttataccact cttgcacagg agccaaccgg atccacagcc
    20941 tgttgcgtca atgcagcgac attcacggtt gcgattgcct gtggtgtggt actattatcg
    21001 atgggaatgt tcctcatccg gaaacatatg agggtatgac gaaggaccgc cctgatatcc
    21061 ggtgagagaa gaggatgcta tggtaagagg atgataagga gattgctatt ttcgtggaag
    21121 gcaccctgtg gtgtttgaaa aaatccaagg gaaggggtgt gaaaaaaaca atgccccgat
    21181 cgctcctgga acgtttctcc cttaatggct tccatatcgt ttcacggcca tgatagggag
    21241 ccccacttcc tggggatccg gagcagaaca acacactccc atcgtactga caagaggttt
    21301 actccgtcga taaatcgtgt aatcatttca aaatctatat cgtatattat accaattgat
    21361 tatacccata tggtgattct tgaatgaaga aaacaaccct gtttctgatc gtactgctcg
    21421 tcttttcaac gatgctgatt gccgggtgca cccagcagac gcccgaaatg aaaccggtga
    21481 caacgccaac agtgaccgaa aaaccgacat taccgcccca ggtgaaccag accgtggcaa
    21541 acattaccga gaaagtcaac cagaccgcgg caaacatcac tgaaaaagcc aaccagaccg
    21601 tggcaaacat taccgagaaa gtcaaccagt ctgcgacaaa cctcaccgag aaagccaacc
    21661 agaccgtagc gaacattacc agccaggcga atcagaccgc agccaacctt accagtcaga
    21721 ccaataagac cgtcgcagat atcgtaaaca agacctaaat ctccctcttt tatatgaaaa
    21781 tgcctccggc attttcatct tttatgcttg tggccctcga aggaatcatg ttattttttt
    21841 agaaggctca attagttcaa gtcattttga tgataccggt gaatggatgt atctctgatc
    21901 ggtacggcac cttccttatt cgcaccaatc gtgtcttccc gcatcactat cacgactgcg
    21961 gcttctcctt tacgacctct ctggccggct tgtcatcccg aagcccaaga taacgggggt
    22021 ggcggagttt tccttcagga gtccactcgg tgaagatcac ttcgcagaca aggaccgggg
    22081 tgacgaaatg ctcccctttc tccggtctct ctttatctgc gaacggggaa gcctgctgtt
    22141 ccagggagac gagtcgggca ccgatctccc tgagggtccg gtcatcaaac cctgtcccca
    22201 cctttcccgc agagagcagg cggccatcct ggtagtatcc caggaggagc gccccgaacc
    22261 ctggcctgct gccttccggg tcggtatatc cgccgatgac aaactcctgc cggttgaggc
    22321 acttgaattt gcgccagtca gaagatcgcc ggtggaggta acgtcccgag gctctcttgg
    22381 cgatgatccc ttcccaacct ctcctgcagg catttcgata gaattccagg ccatcctcct
    22441 ggtgttcgct atagcgaaca gggtccctga aggggatgag tgatgcaaga agggttttcc
    22501 tggtgcggag gtcaaggctg gagagatcga agccgtccag gtacaccagg tcaaagaggt
    22561 agagaacgac cgggatcctg ctcctccggg ccttctccgg gtcgtggatc tgcatccttt
    22621 cctggagccg ggaaaaaatg ggagtctcgc cttccagcgc gacgatctcg ccgtcagcga
    22681 tgaactgtcc gtcggtgatc gcttccaggg cttccaccag ttcggggtag gtatcattca
    22741 ggacgttcct gttccgggag agcagccgga tcctgtgccg gtcgcggaat acgaggcatc
    22801 gctcgccatc gaacttctgc tcgaagatcc agtcgccgct catggatccg ggggatgcca
    22861 gggtagcgag catcggctca atccaggcgg gctgctcgcg ggcgacgagc cttttctgct
    22921 gttcttcggt cagggcggcg aggagggggt gcatcggtga tcggtagttt cttcctgatt
    22981 cttagaacca tatatctgtt tcattgcggg ctgatcgcgt ttgggagtgc tgcccatatc
    23041 ggggacattg taaaatacgc tgatgatgat tcgttggact accgggagat tcctccccga
    23101 tttttttgga gaactcccgc attgaggact cctccccgcc tccgatctct cctcagcacg
    23161 acaccggtgt gatctgccag accagcagca cccacttgtt ctcacgtttt gcgtaatgag
    23221 cagcgacatg aaacgtcccg gaaatcctct gccctctgat cgtgatatcc tcgatgcact
    23281 ggtagatgag gaccgctgca tcgggggaag ccacgaccat gcgtgggtcc gagatgatga
    23341 actcgtgcag cgtgagcgag ggcagaaggg ttgccaggat ctcttttttt gagaatctgc
    23401 cgagtgcgtt gatctcgata aagtccggtg caaggaggat atcgagcgct ctcatattct
    23461 tctccttcca ggcccgtgcc cgggcttttt catgagcaag aaggagggtg gggagctgtc
    23521 cgggagttcc tgttgatgtc ataacaaaag atccatccct ggacgaaaaa gggtatgcca
    23581 ggaagagaga acgatccttc cggaaccgga ttttttttaa taattgacat taaataatgg
    23641 actggggttg tctgccggag tccacgggta tcccatggat ctccgatgat atcatatgtc
    23701 tggatccagg aagagacgag aagaggaccg ttctgattct gctgccggag caacgggttc
    23761 tcctccagca ggagatctga aagccgagaa cgaagcgctc aaaagggaga ttactgccct
    23821 caaagcatct ctggcagcgc cggaaggata cgaagcagtc cttaaagaga gtgatgaaaa
    23881 gttcaggaac ctcctccagg gcatatatga aggaatcatt ctgaatgatg acaaaggtgt
    23941 gatccttgag tggaataccg gcatggagga gatcattggt atctccaggg acgatgcgat
    24001 aggaagaaca atctttgaag ttgcatcacg atgtgttccc gagtccaatg atgcgggcaa
    24061 tgggaaaaag tggttatccg ccataatcga tacgcttgac aggacaccaa cgtcaagatc
    24121 ccatagtcct catctcgaaa tagccgtgaa gcatccagac ggggctctca ggaccatcga
    24181 agtgaaaaat ttttattaca cagtgctggg aagaacattt tacgggggga ttgtccggga
    24241 tattaccgat ctgaaaatga ttctggagaa tctttccgaa cgtgaagatg aattgaacaa
    24301 cctgatccaa tcatcgagtg acgcgattct cctgaccgat gaggagggac gcattaccaa
    24361 gtttaataag gctcaggaac gacttaccgg gcttgccgca gatgaggtgg cggggctgcc
    24421 tgcatgggag ttccaggcat tgaggtcgag cccgaaagga cctggccttg atcccctgac
    24481 ctggtacaag gagatctggg accgcctcct gcatgatgag aacgaccagt tttaccgtga
    24541 aattgttgaa gacgacataa cgagcacgac cggggcagtc catcatgtcc aggtacgggc
    24601 tttcaggatc cccacaaaga agggattccg tgtcggtgcg attgtacggg acatcaccga
    24661 cctgaagcgg acagaagttg cccttctcga gagcgaggag aagtatcgta ccttgatcga
    24721 gatgtccccg gatgccattg tgatccacga tggaggtatt attctctatg caaatccggc
    24781 cactgcacac ctgttccatg cctctcatcc tgagaatatt attggcaggg atgcctttac
    24841 gataatccat cctgattccc aggcaatcag tcggacagtt gccgagaagg ataaagaagg
    24901 gatcagcacc ctcccgaccg agattcaggt tatccgtaag gatgggacta ttatgacggt
    24961 ggaggggaga gggagaagga ttcagtatca gggcaagccg gctatcgaag tgatcctgcg
    25021 ggatgtcacc gagcggaaaa aatctgaaca gcagctgcgt gaatatgccg ccaacctgaa
    25081 acggagcaac gaggaccttg aactcttcgc ctacatcgcc acccacgacc tccaggagcc
    25141 gatacgggga atcgtcgcct attcacagct cctccttgcc gagtgcaggt ctgatcacag
    25201 ctcccagaca aagaaatacc ttgaaaatat cgcccgcgat ggcctcaggc taaacaccct
    25261 ggtggctgat ctccgggaat acctgcgggt acggtccggg ggaagaccgt ttgctccgac
    25321 cgacatggag aggatcctcc aggataccct gcacaccctt catcggcaag tgacggagac
    25381 ccaggccacc atcacccacg acccgctccc catcgttcag gccgacagtt cccagatgac
    25441 ccaggtcctg cagaacctcc ttgcaaacgc catgaaattc cagcagaagg ggattgcacc
    25501 aacagtccat gtctccgcca gttcccttga tgggatgtgg cagttttcgg tgcgggataa
    25561 cggtgtagga attccggaag ggtattttga gaagatattc atcctctttg agcgcctcta
    25621 cccgagggat acctatccgg gaaccggcct tgggcttgcg ttgtgcaagc ggatcgttga
    25681 gcgccacggc ggccggatgt gggttgagtc tgatgagggg agaggatcga ccttctactt
    25741 caccctccca gtcactgctc ctgaatcttc cgtctgaaca ctccggcacc ctgtctggcc
    25801 gcgttttccg gttttcagag gaaccatccg gcgtctttaa acctgatcag gagcgatact
    25861 ggtactacga attcaccagg ggaacggttt gtctgcagga acctcgcaaa atatgttcgg
    25921 tgaactcctc accctctttg catcaacgga gatcttcatc ttcgttttca tggagatcgt
    25981 cacaaagctg gagacgatcc agcgggtgat cttcaaaaaa gcaacggtgc gggattatgc
    26041 ggtatttgtt gttattttcg gcctcttttc aatattcggt acctatatcg gaatcccgtg
    26101 ggagcatggg gctatcagta acatccggga tctcgccccc atcgtggcag gtttcgtggc
    26161 aggtccctac attggtgtgg ctgtcgggct gatcgggggg atccaccgcc tgtttctcgg
    26221 ggggactcgg cagtaccgtg ctgtattgcg acgatccttg caggac
//
LOCUS       DAOOCO010000007        26065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_1562_length_26065_cov_6.002922, whole genome shotgun sequence.
ACCESSION   DAOOCO010000007 DAOOCO010000000
VERSION     DAOOCO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 26065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26065
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_26065_cov_6.002922"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK154_00895"
     CDS             complement(<1..902)
                     /locus_tag="PK154_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09651.1"
                     /translation="MKGETRIPITDGKLPFKHLLTGIIVLIIIILVLAGIGVNYANSA
                     QDLKDNANRLRVMTEMYIDNSLTLNDVGLKLYDTTYNDEMEKAFKVVMQEYNRTGGVP
                     AQMDLEGLKSVIGGMDVYVINDRCIIEYATVPADIGLDFAVIYPDFCQYLHEIQNTSG
                     FYPDRVVNDWNTGTQTKFSYMPTPDHRYILELGLHSERFGGERRQLGHNAINNIWEFN
                     PYLEEVLLFQKQKRLIGNTTYVPTEEDHTMLDYILWENRTSQEIHDNERERTIFWQVI
                     DLRDPTYGADTSIFAKITYNDALLT"
     gene            1115..2089
                     /locus_tag="PK154_00900"
     CDS             1115..2089
                     /locus_tag="PK154_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW09652.1"
                     /translation="MATLSERIPSPSSFERTSNVVLAIAGAFLIAVIVWFATENQIYT
                     IAAALIVGYLLLGVRIVRPTHRVLIEFLGKFQRFGGPGFYWVLPGFQKLYAVNITEKM
                     VNATRQEIITADNLNATVDAQVYFKVKDDELNVKRSQYNVYNYEDQIVNLARTTLRNI
                     IGTLSLKSANSERSRINDELGKVLKTETQSWGIEIVRTELKEIMPPPDVQDTMNKVVK
                     AENEKIAAIDFATARETEADGLRRAAIKQAEGVKQAKILEAEGQAEAIRLVNEAADRF
                     FIGNAQKLKSLEVTESALKTNTKIVLPEGQQIVNVIGELAGSKLGLQE"
     gene            2330..3556
                     /locus_tag="PK154_00905"
     CDS             2330..3556
                     /locus_tag="PK154_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HPW09653.1"
                     /translation="MKILHTSDWHLGNVLHGRKRYDEYERFLHWLVGCIRDEQIDVLL
                     IAGDIFDTTAPSNRAQELYFHFLNRVAAIHGLQVVVTGGNHDSPSLLNAPKPLLRQLR
                     IHVVGAVTPDVSDEVILLQSGDSLESVIVCAVPYLRDRDIRTVEPGESIDDKMTKIQE
                     GINDHYRQVWTVAQSLRGDRAIPIIAMGHLFLAGCSPNGDERVRELYIGTLDGVDPGS
                     ILHGFDYLALGHLHEHRKVAGNPWCRYSGAPIPLGFGEAGQEKKVVVIDLFEGGTRSI
                     REITVPCFLRLQKIQGAISAIEQTLRELGAEEEQVWVEVVAESDSTAAGLQARLHEVV
                     RNSPVEILKIENAALTNAVLRAMEEGEHLGDLDERDVFSRCLDEHGITGGDRDQLFAL
                     FQEILVAVREEDHYAE"
     gene            3566..7246
                     /locus_tag="PK154_00910"
     CDS             3566..7246
                     /locus_tag="PK154_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW09654.1"
                     /translation="MRILKLRFKNLNSLEGEWSIDFTHPAYVSSGIFAITGPTGSGKT
                     TILDAICLALYGQTPRLKTISKGTNEIMTRNTAECFAEVEFESQIGRFVCHWSQRRAY
                     KKPEGELQPPKHEIADKISGKVLENKLQRVAERVIETTGLDFSQFTRSILLAQGDFAA
                     FLNANPDERAPILEKITGTAIYSAISKKVHERNTLEAGKRNELQDRIGILDILSPEEV
                     SALKRNCEDIGAVIRKKSSERDSIRKAAEWLQQLSRFKAEIEALAEERRLLEVKRSDA
                     APDLALLARAKKCQPFEETYRDLTSSRKLSETYDTEIVAAREQLDGTQVSYTASRKAY
                     ESARQRYESLKAAAEEEKQVFIRVRNLDTRIHDIKDQIAEKKIELKKGEASLQQCTRN
                     ITRETERAKEKKAELARAITYLEEHPKDSLLRENVSGLGEKMRHYEQLNRRLLSNRDQ
                     LASQTAEVDRLKKAHETQRTLLEEKDREFQEATAAVRMIEATADQLLDFRKPGFWQAC
                     VASLKERRITLEAELKIHDAIEQIGMKIREREDHQDELKEEIAKKREILSGLKREQDL
                     KEEIRARIETNLALLHRIQSLEEARSHLVEGDPCPLCGSPDHPYARGTIPHPRGVESE
                     WAEIKEELRLLSDQVRQTELSLSQSETTLSSDRRAVTEQRDRIRELLDESAFGFEDIG
                     SVTNSSFLKKSLSHALTLCEVHSAWCSEIVASYEGIVPRLKDASDAATRIKESLSSHQ
                     TSLGNLDVSLKIRSNTLYELEQQVASDAEELARLYQEVAAQLDGFGMTFTEGTSITGI
                     LESLEKRKREFEKRATRKTDIEKEIDLIAGSILNHQKQREDTRVAVASQLQGISEKEG
                     VFVALDHERKELFGTKDPDREEKRSAEELRSAEEQFREASSASKEYEIRITTLQEQIE
                     KKSGTLEQILITVRDHTSSFLAGIQKAGFSGEEEFIESRMPRDRFEVLEKRETDLIFE
                     ERDLSSRIKERTTALSYERSRNLTDKSPDTLEEAISSLESDITGLHQEQGSIRQKLTD
                     HEARVAHQNELVQALQMQEAECLRWEKLHDLIGSSDGKKFRVFAQGLTFDRLIAHANR
                     HLRKMSDRYLLIRSGRSSLDLDIVDNYQAGEIRSTKNLSGGESFIVSLSLALGLSGMA
                     SRNVRIDSLFLDEGFGTLDSETLEVALETLSTLQQEGKIIGIISHVPALKERIPTKIV
                     VEKMAGGRSRLKAPGCRPPETP"
     gene            7335..10442
                     /locus_tag="PK154_00915"
     CDS             7335..10442
                     /locus_tag="PK154_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPW09655.1"
                     /translation="MAARSSVIRGLPGDHLEAFISDFKAGSECDPFSTWLILPTERLV
                     QRVRDELSLKNVPFIPSRICTLKGFCRSYFAENRRTTRELTKAESRLLLSLIVSENRD
                     VLPLFFVRERPLPGTIEDLQTFMNVITTRKVAFPECLGDLQGEKSDQIARIISAYRNR
                     MKEHDLVDTDTILEWTIHHLENGGAKPPRHLFVYGLFGPLPLEEDLLSLLRERSESFT
                     CFIPSGADPAIFARSPGWTGMGVTGSLVSPLPSTPSLLTGIFSSPEPIGIGESIRLDT
                     FPSHYAELQAIAKEICRLNDHGVPLSDISVAFPELQDEIGSITEVFSDFGIRWNSRVT
                     PKLSRVPVIRFLVGIIGLVVNRYPREEVISFLNSPYLQGSSPRDLPDPSEADLVSRHA
                     CIESGRNEWRNRLEYLTSSIANGSEKRYHGISQHSVDRVREGMFTLFGALEGLESRRP
                     VQDFIQEYTGFLDRYQLPYLPPAPGDQEKIIDEDRVVGIFRERLEALCNNPFFTRDRK
                     VTTDEFLRIVNTLVDEPRQESDPERGGVSVIGIREAMHLYLPVLFICGLVEGVIPRLT
                     TRLPFTNSLENARMGTRSLAEILLEQEYYFIAALLSSQRVYLSAPLAEGSTPLLTSAF
                     FERVRMVCHPKGWSSTGQDAECCLRSLAAVTAGERIAEGLVCQAIDRLDPLQSIDDLV
                     ARINTERYYRKGTCDSIYEGMLSADKTIAAALGERFSPEGVYSATSLETYAQCPFRFF
                     IGNVLRLEDLPDGEQNLSPADRGSAVHDILSTFYRRWLAAGQGHITPASIEAATSLMQ
                     EIAREELDRHHFESPLWDATCLQMKGSPHAGPGYLEKFLAKEAEAADSPLAPCHFEFS
                     FGMNPSGTDDPASVEEAVALTSSDGREQLRIRGRIDRIDISPEGFFLIYDYKTGRGHP
                     KIKDIEEGKALQLPLYLLAFEALSEKRGVAAGYYHIRKEVRRDLLLCDEQARCLVASR
                     TKMTPDVAALITRSCDFAIGYIRRIREGAFPLHAEEHCPNSHCEFRRICRFDPYRVSD
                     SGEVT"
     gene            10442..13777
                     /locus_tag="PK154_00920"
     CDS             10442..13777
                     /locus_tag="PK154_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW09656.1"
                     /translation="MAATRRQIEAISTHDRSLVVTAGAGTGKTYVLVQKYLDLLETRG
                     VTVPEILALTFTEKAAAEMKERIRRALSGKKGERWERAAVDFLVAPVQTFHSFCAQVL
                     REFPIESGLEPGFVVLDERQVSRIHGKAFEDLVHVPSPPPVHDAVVRILSVFEPSTVR
                     AMLAEMYGKRLSYRQFFEALGSDSDQVIRTWTREVNTVRENGIRDLRNDRLFNAALTT
                     LFSLADRYEGADDKAVCFLCGIRPLLEAISGDSGIEAFCSAATDLLTKRPGNVGSRKV
                     WKGNDLSLFKAAYKELNDILKRKSSLFRMTVEPDDPLVTRAIDLLGDLSLVYLRYTEL
                     VEREKAVLGGLDFEDLILHARQLFLTNEALVATHFLPRFRYILIDEFQDTDPSQFDII
                     LAIVGKPGPSTDCLFIVGDPKQSIYLFRDADVTRFKAVREIILNRCQGREVHLDTSFR
                     STKEVIGCANYLFSRLFASAEKPWEFGYEPILVSEARSGDAGSVELLLPPSGEDSAAT
                     KWIEADMVARRIQSLVQGCHVQVYEEAEDRSFISRPARYGDIAILLEQRTNLSSYLAA
                     LARYGIPFYVYGGTGFYSRQEVYDLYNLLRFLHFRHDDISLAGTLRSPYCGLPDTDLF
                     LISREKGRTFWEKLQNYGEKTGSEGAVRACRLLSLWRADAGRTGIVPLIRRILSESGI
                     YTVYAALPEGKQVLANLEKIVAMARVREGAGSYGLADFIEDLRTAMDEEEREGEAPLD
                     ALAENSVNVMTVHAAKGLEFPIVVVPDMGMQFRETFPPIMIGDNPLLVGITVPDPEEE
                     FELTKTPMLLALRELRRQKERAEKKRLLYVALTRARDHLIMSGTAPTHPDLSLSLATS
                     RIEWIFSALGITGDAIAAGGMDLDARDQMVRLAITSDPAAIPAEAVEITPELLVVPKE
                     CAGKNGTWSPKEFDKGPEGIRIVSVTDLEKEQSSHSHSPTKTVGPRYLPGVDGTTKGT
                     VIHEVFRGRDARVVCLEYGVSDPEAIRQCEEMVSRFLSSPLMQRVKREFCEMPFVITY
                     QGKHVTGKIDRLCELVDGTWVVIDYKSDPVDPSEYSKKTGKYQKSMEVYFEAARQLVK
                     RNEVEAYLYFTETGEFFNAKNGF"
     gene            13859..15559
                     /locus_tag="PK154_00925"
     CDS             13859..15559
                     /locus_tag="PK154_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW09657.1"
                     /translation="MIISKINLHNYRGIHDINFELQNYSLFVGPNNSGKSTLVNAIRA
                     FYEKDGFKYRKDTDTPLIHPKDKESWIEIHFKLTNEENESLAEEYKQNNNQLFVRKYF
                     DTETKTHDGKSANGIIFGYNSEGMLSDEPFYGAKNVQSGKFGDIIYIPAVSKVDEHTK
                     LTGPSALRDLVQNILSSTITESGNYDEFKEQVSIFSEGLRQEKTKEGYSIQSFEGELN
                     ESLNPWGVKFKIKLKTPAPHEIVKAMLDYDFEDMMHGKTQLIDNYGSGFQRHFIYSLI
                     NISSHYTFIKEKKKSKDFSPSMTLILFEEPEAFLHPSQQDILARNLMKFSDPDINQVV
                     CSTHSPHFVSRNSNNIPGLIHFSNENGIIKTYQIGDDEWKSIVDSNQIVNKIADKYPK
                     MKKRLQEDDLKPEMESVKNFLWLNPDRCGIFFANHAVLVEGPSEVALINKLIGDNKIK
                     KSNCGLYVMDCIGKYNIHRFMNLISSLGIKHSVIFDDDDNQEEHLEFNQLIEESRNPL
                     FTYAIKKIPKNLESFLSIPNAGSDHRKPQHILYLYECGSIAKDKIDSFCGMIDSCLPN
                     "
     gene            complement(15664..16287)
                     /locus_tag="PK154_00930"
     CDS             complement(15664..16287)
                     /locus_tag="PK154_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09658.1"
                     /translation="MAEIHDLIKEGIKISKRIQNIPFSDLLNATTPFEVYQLDLTNIH
                     DLELLTEIQSSANNFLKYVDRTHQRFEGRRINEVGRRIEEVFVEELKKTKLKPIQLSA
                     SGYPDIKIVDNQNRVTFLESKAVSQDWKSSFRSFYYTSGKKIDSNGRHLLIAWDISNE
                     TGNYWKVNGWKLVDLANLIITTKLEFQSNNQKLYDPTLIIQESNKHS"
     gene            complement(16323..17183)
                     /locus_tag="PK154_00935"
     CDS             complement(16323..17183)
                     /locus_tag="PK154_00935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW09659.1"
                     /translation="MKKIKPEFNKIYNKDCLKGLQSFDEKIVDVVVTSPPYNIGIKYH
                     EYKDTKSRDEYLSWLEQVAISCKRIMKDDGSFFLNVGNTASDPWIAIDVANQFRRHFV
                     LQNMIHWIKSIAIPRESMGNYPHIKGNIAVGHYKPITSVRYHNQCHEFIFHFTKTGNT
                     KLDKLAIGVPYQDKSNIQRWKSKTGDVRDRGNTWFIPYETIKESRPHPSVFPVCLPQM
                     CIKDHGIDKTELVLDPFMGIGTTAIAAIREDKAFFGFEIDKEYLKIARDWISVEVKKK
                     KDKENQKKIL"
     gene            complement(17320..18402)
                     /locus_tag="PK154_00940"
     CDS             complement(17320..18402)
                     /locus_tag="PK154_00940"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HPW09660.1"
                     /translation="MTPEEIRRLRLSHSGLSHSPFTTGAEAVAHSGAVQAQDFSAAIW
                     SLGLRIRDATAATIEDAYNDGKILRVHVLRHTWHFILPEDLRWMTALTAPGVKSMVAA
                     SNRKLGLDPTLFSKSNAAIEKALEDHHVLTRQDLKGILADIGIVTDVQRLAHIIMQAE
                     LEGLICSGPMQGRQHTYALADERIRESRALTREQSLAELAHRYFTSHGPAQLKDFSWW
                     SGLSRRDAGVGLDSIREELERASINGNTHWYPPDPGEVIPDPPVALLLSIYDEYAIAY
                     ADRSAISDAREIERMITKGNAMTAVIVLHGKVAGTWRRTMKKDSIEVRLNPFKELTDE
                     EQEAVEREVERYGKFFGRGVEIMSGN"
     gene            complement(18586..18996)
                     /locus_tag="PK154_00945"
     CDS             complement(18586..18996)
                     /locus_tag="PK154_00945"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW09661.1"
                     /translation="MNRIIHFEIQADDPGRAAQFYRDVFGWEIREWVIPGIEIKDENR
                     YWFIITGPETDPGINGGLVFRWGPAPVEGQAVNAYTCTIEVPDLDRSVDLVRKAGGSI
                     ILPRMGIPGIGWWAHCRDTEGNLFGMLQEDPNAL"
     gene            complement(19078..20061)
                     /locus_tag="PK154_00950"
     CDS             complement(19078..20061)
                     /locus_tag="PK154_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPW09662.1"
                     /translation="MQKTMTKRKSAGLSITRVFDAPVDLVWKAWTDPGYVKRWWGPKD
                     FTAPFIMIDFRVGGTSLLCMRSPDGQEFWSTGIYREIVPEKRIVTTDSFSDEKGAIVP
                     ASFYGMTGVWPLELLVTLTFEEKNGKTTFTLVHEGMPPGEPSEQAKAGWNESLDKLET
                     LLHDETQHREKNVLVIEPGKPASLTRIFDAPPERLFSAVTDPARIPLWWGPGNLTTIV
                     DTMDARSGGSWRFIQRDADGNEYAFHGVYHEVSPRRIVQTLEFEGMPGHVQLEVITFE
                     EVDGKTKMVDKSVIETPEDVEDEMLKSAMAEGWMETIDRLAELVEKKWREG"
     gene            complement(20046..20303)
                     /locus_tag="PK154_00955"
     CDS             complement(20046..20303)
                     /locus_tag="PK154_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09663.1"
                     /translation="MDLMRGISCANIPFFNSVPVKDPGTGQESPGLTGKIPIQHFFSV
                     LLILVQNLPEDWQILKLPVKFLITGNNKTAGTNGGIPCRKQ"
     gene            complement(20428..21063)
                     /locus_tag="PK154_00960"
     CDS             complement(20428..21063)
                     /locus_tag="PK154_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HPW09664.1"
                     /translation="MDREEVTRQCLLLGSMKTRGELGQAIAGIVLSYSPKDLQQMRWN
                     FSGAIQDINPEYRKKLEETITAHLHGTYQALRLMKQQGSFAPMNQPLEKKTEEYWTMV
                     TCQCRSGDEEQVRLRFLKFLLSGFCMLVQEIPGHPVGTPFPGGDKVEIIDGTYYCPVR
                     DKANDVDSALCPFCPALQTPEVGYLKPPVKGSDHRKKEFLRQTFDRHYYNG"
     gene            21283..21459
                     /locus_tag="PK154_00965"
     CDS             21283..21459
                     /locus_tag="PK154_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW09665.1"
                     /translation="MGNTIDRYRCTVCSYLYDPAVGDPGSGIPAGVLFEDLPEDRVCP
                     ECGVGKDQFERVKE"
     gene            21456..21815
                     /locus_tag="PK154_00970"
     CDS             21456..21815
                     /locus_tag="PK154_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPW09666.1"
                     /translation="MIRVKRIYDVPGEGDGFRVLVERLWPRGMRKEQAGIDLWIRDVG
                     ASPALRKWFGHDPAKWEEFQEKYFHELSGKPELGELLTALGRDHPVITFLFAAKDEEH
                     NNAVALKKYLDMRGELG"
     gene            21812..22036
                     /locus_tag="PK154_00975"
     CDS             21812..22036
                     /locus_tag="PK154_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPW09667.1"
                     /translation="MKVIIDRLNCVSCGTCWELCPDFFEENPDDSFSQIREKFRLDGL
                     NGEGIPSGELEPCVVDAADLCPASVISIKE"
     gene            22484..23779
                     /locus_tag="PK154_00980"
     CDS             22484..23779
                     /locus_tag="PK154_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW09668.1"
                     /translation="MELPRCLKRGLAGIIGSAGLAYPVCAAICPRGKGYCPYPGRCFL
                     YTDVDTNAICDYTPRSVTTAPTAASPTPAASPVVEATSSLSTPVTSSVTPAATGPASV
                     TPAATVSSNASVPFTPDAGFFVPDGLIMGAVATIFLVLLFFFLFRSGILGSRLQKRVP
                     ALGASALFALGIGEMVTYLLLGEETLASAFSVIYLLFGTILAAWVWKSGAMSRKLAVA
                     TVMISALFGFLLLAPLMPMEFPGIVSIATSGQTLAPGIIGILAGIGLVLLVGRTFCGH
                     LCPVGSVQELAWNVPGKKIDIRKTRYLEVFRAGVFAATVIGALYYVNLMEYTGIYDFF
                     SLTLSIGLVVFFVLLLLSIFVYRPVCRGICPFGLLFSIPAHFSLFRLRRTSACISCKK
                     CEKACPAHVAGKDSSKRECYLCARCTAACPVQGALVYKR"
     gene            24059..24415
                     /locus_tag="PK154_00985"
     CDS             24059..24415
                     /locus_tag="PK154_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09669.1"
                     /translation="MIDMTTITDEFMSEMIGRTKDYSIVLLRIGPNRYMAGVEAIIWE
                     HARRNFSLRSSGKLPIVCPVNDGSELCGVGIFATGPEETRNIMNEDPGVMKGIFSYEL
                     HPCRSFPGDRLPGQLG"
     gene            24952..>26065
                     /locus_tag="PK154_00990"
     CDS             24952..>26065
                     /locus_tag="PK154_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HPW09670.1"
                     /translation="MKIDHFFTMLLLLIAFLMLIGNVQGISQDDLNNIVIRDVLNEKM
                     EGMWVYAKPEPVEAGSTITTWYGDILLPEKEGWLFFIDDAPMQSWAHPCRYIFVDEAG
                     SVTVRDALGPPMDLAKWKKVAGVATPLTSPLSAMVPSAIRGSINALPPCMNPGHCYAV
                     IISGGADSYNNWERYYGDVSFMYKTLVNDYGYLDDHIIVLMSDGTDAGLDQHKYDDTY
                     VNSNPDLDGDGDDDVGFSATNANIGTVFDDLQTRLENSDHLFIFTTDHGGPENDPQVG
                     TDVIINLWYETMRDDQFDANLDKITATVPIMITMEQCYSGGFIDDVIPGPVGQERVIA
                     TAANAFESSWGDTFSTLWISAVAGHDKGGASVDADTN"
BASE COUNT         6291 a         6672 c         6807 g         6295 t
ORIGIN      
        1 gtcaggagcg cgtcattgta ggtgattttt gcaaaaatgc tggtatccgc tccataggtg
       61 ggatcgcgga gatctatcac ctgccagaag atggtccgtt cccgttcatt gtcatggatc
      121 tcctggctgg tccggttctc ccagaggata taatcaagca tggtatggtc ctcctctgtg
      181 ggaacatacg tggtattccc tatcagccgc ttttgcttct ggaacagcaa cacctcttcg
      241 aggtatggat tgaattccca gatattattg atggcattat ggccgagctg tctccgctcc
      301 ccaccaaaac gttccgagtg gagcccgagc tcaaggatgt atcgatgatc aggggttggc
      361 atataactga attttgtctg ggttcctgtg ttccagtcat tgaccacccg gtcggggtag
      421 aatcccgaag tattttgtat ctcatggagg tactggcaga agtcagggta gatgactgca
      481 aagtccagcc cgatatcggc aggcacggtc gcatattcga tgatgcacct gtcattgatg
      541 acatagacat ccatgccgcc tatgacagat ttaaggccct cgaggtccat ttgtgcagga
      601 actccacccg ttctgttgta ttcctgcatc actaccttga atgccttctc catctcgtcg
      661 ttgtaggtcg tatcatagag tttgagtccg acgtcgttca atgttaagga gttgtcgatg
      721 tacatctcgg tcatgactct gagccgattt gcgttatcct tgagatcctg ggcagaattt
      781 gcatagttga ccccaatacc agcaagaacg agtatgataa tgataagaac aatgatcccg
      841 gtgaggagat gcttgaacgg cagctttcca tcggtgattg gtatccgggt ctcccccttc
      901 actgatcata gaaattcaag tcataaaaaa tgatatggat cgttcttatc atatcgaaga
      961 atctgagtga aagattcgca ttcccgtgca tggatggtcc tgtttggtat ggaggagatc
     1021 ttattgtatc cctcgcgatc tcccgctttc ctttcaacac ccccgccaac acaataccta
     1081 tcgtatcagc catcaaacag gctgaatgat cgttatggca acactttccg agcgaattcc
     1141 ctccccctcc agtttcgaga ggacctccaa tgtcgttctc gcaatagcag gggcattcct
     1201 gatcgcggtt attgtgtggt ttgccacaga gaaccagatc tataccattg ctgcggccct
     1261 gatcgtgggc tacctcctgc tcggggtcag gatcgtccgg cccacccacc gggtgctgat
     1321 cgagtttctc ggaaaattcc agaggttcgg cggtccggga ttctactggg tcctccctgg
     1381 tttccagaag ctctacgcgg tgaacatcac tgaaaagatg gtgaatgcca cccggcagga
     1441 gatcatcacc gctgacaacc tgaacgcgac cgtagatgcc caggtctact tcaaggtgaa
     1501 ggatgacgag ctgaacgtga agcggtccca gtacaatgtc tacaactacg aggaccagat
     1561 cgtcaacctg gcccggacca ccctccgtaa catcatcggt acgctctctt taaaatctgc
     1621 caacagtgag cgttcacgga tcaatgatga gctcgggaag gtactgaaga ccgagaccca
     1681 gtcgtggggg atcgagatcg tcaggaccga gctgaaggag atcatgcctc cgcccgatgt
     1741 gcaggacacg atgaacaagg tcgtcaaggc cgagaacgag aagattgccg cgatcgactt
     1801 tgcaactgcc cgggagaccg aagctgatgg gctgcgcagg gctgccatca agcaggcgga
     1861 aggggtgaaa caggccaaga ttctcgaggc cgaggggcag gctgaggcga tccggctggt
     1921 gaacgaggct gccgaccggt tctttatcgg caacgcccag aagctgaagt cgcttgaggt
     1981 gaccgagagc gctttaaaga cgaataccaa gatcgtcctt ccggaaggcc agcagatcgt
     2041 gaacgtgatc ggggagcttg ccgggagcaa gctggggttg caggagtaga aaaaccaaca
     2101 tcctttttcg ggtatccgtg cacatgaatg tcttactccc ccaggtctat tgattttgag
     2161 tgcagttgtt cgaaggaata ataatacccg ggtattcctc actccatcag cgaactggtt
     2221 ttgcctggtc tttcctccct cgatccattt tctccccgca cttctccatc ttatatagga
     2281 gcagttcttt accatctatc aatcaattac ctccaaccgg agaccgtgca tgaaaatcct
     2341 ccatacttct gattggcatc ttggaaatgt gttgcatggg aggaagaggt atgacgagta
     2401 cgaacggttt ctccactggc tggtcgggtg tatccgggat gagcagatcg atgttctcct
     2461 gattgcgggg gatatctttg acacaactgc cccgagcaac cgtgcacagg aactgtactt
     2521 ccattttctc aaccgggtgg cggcaatcca tggcctccag gtcgtggtta ccggtggaaa
     2581 tcacgactcc ccgtcattgt tgaatgcccc aaagcccctc ctccggcagt tacgcatcca
     2641 cgtggtaggg gcagtcaccc cggatgtatc agacgaggtg atccttctcc aatcagggga
     2701 tagcttggaa tccgtgattg tgtgtgcggt cccctatctc cgtgaccggg atataaggac
     2761 ggttgagccc ggcgagagta tcgatgacaa aatgacgaag atccaggaag ggatcaacga
     2821 tcattaccgg caggtgtgga ccgtggcaca gtcgctcagg ggcgatagag cgataccgat
     2881 catcgccatg gggcatctct tcctcgccgg gtgctcccca aacggggatg aaagggtccg
     2941 ggagctgtat atcggcaccc ttgatggagt tgatccgggc agcatcctcc atggattcga
     3001 ctacctggca ctgggacacc tccacgagca ccggaaggtc gcaggaaatc cgtggtgccg
     3061 gtattccgga gcaccgattc ccctgggatt tggtgaagcc gggcaggaga agaaggtggt
     3121 cgtcatcgat ctttttgaag ggggaacccg ttcgatacgg gagatcaccg tcccctgctt
     3181 tctgcggctg cagaagatcc agggagcgat ctctgcaatt gagcagacac tgcgggaatt
     3241 aggtgcagaa gaggagcagg tctgggtcga ggtcgtggcc gaatccgata gcactgcagc
     3301 gggactccag gcacgccttc atgaggtggt gagaaactca cccgtggaga tcctgaagat
     3361 tgagaatgct gctctcacga atgcggtatt acgtgccatg gaggaagggg agcatcttgg
     3421 agaccttgac gagagggatg tcttctcccg ctgccttgat gaacacggga taacaggtgg
     3481 ggaccgggat cagctctttg ccctgttcca ggagatcctg gttgcggtcc gggaagaaga
     3541 ccactatgca gaatgagggt acacgatgcg gatactgaag ctccgtttca agaacctgaa
     3601 ttctcttgag ggagaatgga gcatcgattt tacacaccct gcctatgtct cttcaggtat
     3661 atttgctata acgggaccaa ccggatcagg gaagaccacc atcctcgatg cgatctgcct
     3721 ggccctctac gggcagactc cccgtctgaa aacgatctca aagggcacga acgagatcat
     3781 gacccggaat accgctgaat gttttgcaga ggttgagttc gagtcccaga tcggccgctt
     3841 tgtctgccac tggagccagc gtcgggcata caaaaaacca gagggagaac tgcagcctcc
     3901 aaagcacgag attgccgaca agatctcagg aaaagtcctt gagaacaagc tgcagagagt
     3961 agcagagagg gtcatcgaga ccacggggct tgatttttcc cagttcaccc gctcgatcct
     4021 cctcgcacag ggtgattttg ctgcgttcct gaatgcaaat cccgatgaac gggccccgat
     4081 tctcgaaaag atcacgggaa ctgcgatcta cagcgcgatc tcaaaaaaag tgcatgagag
     4141 aaataccctg gaggctggga aaaggaacga gctccaggac cggattggca ttctcgatat
     4201 cctctcacca gaggaagtct cggctctgaa gcgtaattgt gaggatatcg gggcagtcat
     4261 acggaaaaag tcatctgaac gtgactcgat caggaaggct gcagaatggc tgcagcagct
     4321 ctcccggttt aaggcggaga tcgaagccct tgcagaggag aggcgattgc tcgaggtgaa
     4381 aaggtcggat gctgcacccg atcttgccct tcttgcccgg gcaaagaagt gccagccgtt
     4441 cgaagagacc tatcgggatc tcacctcttc aagaaaactc tctgagacct atgatactga
     4501 gattgttgca gcgagggaac aattggatgg tacccaggtt tcctatactg catcgcggaa
     4561 ggcgtacgag agcgcacggc aacggtatga atcgctgaag gctgctgccg aagaggagaa
     4621 acaggttttc atccgggtcc ggaatctcga cacccggatt catgacataa aagatcagat
     4681 tgccgagaag aagatcgagc tgaagaaggg cgaggccagc ctccagcagt gcacacggaa
     4741 tatcaccagg gagaccgaac gggccaagga gaagaaagcc gagcttgcca gggccatcac
     4801 ctaccttgag gagcatccaa aagattccct cctgcgggag aacgtatcgg gtcttggtga
     4861 gaaaatgcgg cactatgaac agctgaaccg acgtcttctg tcgaacaggg accagctggc
     4921 atcacagact gccgaagtgg accggctcaa aaaagcccat gaaacccaga gaacactcct
     4981 ggaggagaaa gatcgggagt ttcaggaggc aactgcggca gtacggatga ttgaggcgac
     5041 tgccgatcaa ctgctggatt tccgcaagcc cggattctgg caggcttgtg tggcatccct
     5101 gaaagaacga cggataacac tagaggccga gctgaagatc catgatgcta tcgagcagat
     5161 cgggatgaag atccgggaac gtgaggacca ccaggacgag ctaaaggagg agatcgcaaa
     5221 gaaacgggag atcctgtcag gcctgaagcg tgaacaggat ctgaaagaag agatccgtgc
     5281 ccggatcgag accaacctcg cgctcctcca ccgcatccaa agccttgaag aggctcgaag
     5341 tcacctcgtg gagggcgacc cctgcccgtt gtgcggctct cctgatcatc cttatgcccg
     5401 ggggaccatc cctcatcctc gaggtgttga atctgaatgg gcagagatca aagaggagtt
     5461 gagactactc tccgaccagg tacgacaaac ggaactctct ctctctcaat cagaaacaac
     5521 cctctcatct gatagaagag cggtaacgga acaaagggat cggatccgtg aactgcttga
     5581 tgaatcagca ttcgggttcg aggatatcgg atctgtcacg aactccagct tccttaaaaa
     5641 gtccctctcc catgccctca cgctctgtga ggtccattct gcatggtgtt ccgaaatcgt
     5701 cgcatcgtat gaaggcatag tgccgcggtt gaaggacgcc tccgatgccg cgacccggat
     5761 aaaagaaagt ctctccagtc atcagacttc gttggggaac cttgacgtat cgttgaaaat
     5821 tcgctcaaac accctgtatg agctggagca gcaggttgca tcggatgcag aggaactggc
     5881 acgattatat caggaagttg ccgcgcaact tgatggattt ggaatgacgt ttacggaggg
     5941 gacgtcgatc accgggatcc tggaatccct ggagaagagg aagcgggagt tcgagaagag
     6001 ggcgacccgg aaaacggaca ttgaaaagga gattgacctc attgcgggca gcatactcaa
     6061 ccaccagaag cagagggagg ataccagagt ggccgttgcg tcgcagttgc agggtatcag
     6121 cgaaaaagag ggggtgttcg tcgcattaga ccacgagagg aaggaattat ttggcaccaa
     6181 ggatcccgac cgcgaagaga aacgatcggc tgaggaactc cgttctgccg aggaacaatt
     6241 cagggaggct tccagtgcgt ccaaagagta tgaaatccgc attacaacgt tacaggagca
     6301 gatcgagaag aagagcggaa ctctggagca gatcctgatc acagtcaggg atcatacctc
     6361 ctcttttctc gcaggcatac agaaggcagg attctcggga gaggaggagt tcatcgaatc
     6421 acggatgccc cgggaccggt ttgaggtgct cgagaagagg gagactgacc tgatcttcga
     6481 ggaacgggat ctctcatcac ggataaagga aagaacgact gccctctctt atgagcgatc
     6541 ccggaacctg accgataagt cccctgatac tctggaagag gcgatctctt ctctcgaatc
     6601 ggatataacc ggtcttcacc aggaacaggg gagtatccgc cagaagttga ccgatcatga
     6661 ggcacgggtt gcccaccaga acgagctggt tcaggctctc cagatgcagg aggccgaatg
     6721 cctccgctgg gagaagctgc acgatctgat cggatcatcg gatgggaaaa aattccgggt
     6781 ctttgcccag ggtctcacct ttgaccgcct catcgcccat gcgaaccggc acctccggaa
     6841 gatgagcgac cggtatctcc tcatccggag cgggagatcc tcccttgacc tcgatatcgt
     6901 ggacaattac caggcagggg agatccgctc cacgaagaac ctttccgggg gggagagttt
     6961 cattgtcagc ctctccctgg cccttggact ctctggcatg gcaagccgga atgtccggat
     7021 cgattcgttg ttcctcgatg aaggattcgg aaccctggac agtgagaccc tcgaggtagc
     7081 cctcgagacc ctctccactc tgcagcaaga ggggaagatc atcgggatca tctcccatgt
     7141 cccggcgctc aaggagcgga tcccgacaaa gatcgtggtc gagaagatgg cgggcgggag
     7201 gagccggctc aaagccccgg ggtgcaggcc tcctgaaacg ccgtaatcgg tctgattgag
     7261 gattttcgac caatcggata atttcgacga aaggaatcgt tcaagtctcc aaccgcgtaa
     7321 tatggtatta tccaatggca gcccgcagtt cggttatccg gggtctccct ggggatcatc
     7381 tggaagcgtt catcagtgac ttcaaggccg ggagtgaatg cgatcccttc tctacctggc
     7441 ttatccttcc taccgaacgg ctcgtgcagc gtgtcaggga tgaactctcc ctaaaaaatg
     7501 ttcccttcat cccttcccgg atctgcaccc tgaaagggtt ctgcaggagt tattttgcgg
     7561 agaacaggag aacgacgcgt gaactgacga aggcagagtc acgcctgctc ctctcattga
     7621 tcgtctctga gaaccgggat gtgctcccgt tgttctttgt ccgggaacgt cccctccccg
     7681 gaacgatcga ggatctccaa accttcatga atgttatcac gacgagaaag gttgccttcc
     7741 cggaatgcct cggggatctc cagggtgaga agagtgacca gattgccagg atcatttctg
     7801 cttaccggaa ccggatgaag gagcatgatc tcgtcgatac tgatacaatc ctcgaatgga
     7861 cgatccatca cctggaaaat ggaggtgcaa agcctccccg ccatctgttt gtgtacggac
     7921 ttttcgggcc tcttcctctt gaggaggatc tcctctccct gctccgggag cgatcagaat
     7981 cgttcacctg tttcatcccg tcaggtgccg atcctgccat ttttgcccga tctcctggct
     8041 ggacgggaat gggtgtaacc gggtcactcg tttcgcccct cccctccact ccgtcattac
     8101 taaccgggat attctcatcg cctgaaccga tcggaatcgg tgagtctatc aggttggata
     8161 cctttccctc gcactatgct gaactccagg cgattgcaaa agagatctgc cgcctcaacg
     8221 accacggtgt tcccctctcg gatatctcgg ttgcgttccc ggaattgcag gatgaaatcg
     8281 gcagtatcac agaggtattt tctgattttg gaattcgctg gaattcacgt gttactccaa
     8341 aattatccag agtaccggta atccggttcc tcgtggggat aatagggctt gtcgtcaacc
     8401 ggtatccccg ggaggaggtc atctcgtttt tgaacagccc ttacctgcag gggtcatcac
     8461 cacgagatct accggatcca tcggaggcag acctcgtctc acgccatgcc tgcatagaat
     8521 cgggaaggaa tgaatggcgg aatcgtctgg aatatctcac ttcatcgatc gcaaacggaa
     8581 gcgagaaaag ataccatgga atcagccagc attcggtcga ccgggtgaga gaagggatgt
     8641 tcacgctctt tggggcactg gaggggctgg aaagtagaag acccgttcag gattttatcc
     8701 aggaatatac aggatttctc gatcggtatc agctgccgta cctccctcct gcacccgggg
     8761 accaggaaaa gatcatagat gaggaccgtg ttgtcggtat ctttcgtgaa cggctggaag
     8821 ctttgtgcaa caacccgttc ttcacccggg acaggaaggt cacaaccgat gaattccttc
     8881 gcattgtgaa tacccttgtt gacgaaccac gccaggagtc tgacccagag aggggcgggg
     8941 tatcggttat cggaatccgt gaggcaatgc acctatatct gccggtattg ttcatctgcg
     9001 ggttggttga aggggtcatt ccccgactga ctacccggct ccctttcacc aattcgcttg
     9061 agaacgcacg gatggggacc cggtcgcttg cggaaatcct ccttgaacag gagtattact
     9121 ttatcgcggc gctcctctct tcacagcgtg tctatctgag tgcacccctc gctgaagggt
     9181 ccacccccct cctcacctct gcattcttcg aacgcgtacg gatggtatgc catccgaagg
     9241 ggtggagcag tactggccag gatgcggagt gctgtttacg cagtcttgcg gcagtcactg
     9301 ccggagaaag gattgcagaa gggctggtat gccaggctat tgatcgtctg gatccactgc
     9361 agagtatcga cgacctggta gcgcgtatca ataccgagcg ctactaccgg aagggaacct
     9421 gtgactccat ctacgaggga atgctgtccg ctgacaaaac tatcgctgct gctctcgggg
     9481 agcggttcag ccccgagggg gtatattccg caacgagcct cgagacctat gcgcagtgtc
     9541 ccttcaggtt tttcattggg aatgtcctcc ggctggaaga tctccctgat ggtgagcaga
     9601 acctctctcc ggcagaccgg ggaagtgcag tccatgacat cctctcgaca ttttatcgcc
     9661 ggtggcttgc agcgggacag gggcacatca cccccgcttc gatagaagca gcaaccagtc
     9721 tgatgcagga gattgcccgg gaagagcttg accgacacca tttcgagagt cccctctggg
     9781 atgcaacctg tctgcagatg aagggctctc cccatgccgg ccccgggtac cttgagaagt
     9841 tcctcgctaa ggaagcggaa gcagcagatt ccccgctggc tccctgccat tttgaattct
     9901 cttttgggat gaacccctct ggaaccgatg acccggcctc ggtggaagaa gcagttgccc
     9961 tcaccagttc cgatgggcgt gaacagctcc ggatacgggg tcggatcgat aggatcgata
    10021 tctcccctga aggattcttc cttatctatg actacaagac cgggcgcggg catccaaaaa
    10081 tcaaggatat cgaggaaggg aaagccctcc agctccccct ctacctgctt gccttcgaag
    10141 cgctttcgga aaaacgcggg gtagccgcag gatactacca tatccggaag gaggtccggc
    10201 gcgatctgct cctctgcgat gaacaggcac ggtgccttgt cgcatcccgg acaaagatga
    10261 caccggatgt cgccgccctg atcacccgtt cctgcgattt tgccatcggc tatatacgca
    10321 ggatcaggga gggggcgttt cccctccatg ccgaggagca ctgcccaaac tcccattgcg
    10381 agttcaggcg catatgccgg ttcgatccct acagggtctc tgattccggg gaggtgacct
    10441 gatggccgca acaagacggc agatcgaggc gatctccacc catgacagga gtcttgtggt
    10501 cactgcgggg gcgggtaccg gaaagaccta tgtcctcgtg cagaaatacc tcgaccttct
    10561 cgagaccagg ggagtgacgg tccccgagat ccttgccctc acgttcacag aaaaggctgc
    10621 tgccgagatg aaagaacgga tacggagagc actatcagga aaaaaaggag aacgatggga
    10681 gcgggcggcg gtggacttcc tggtcgcccc tgtccagacc ttccactcct tctgtgccca
    10741 ggtccttcgc gaatttccca ttgaatccgg cctcgaaccg ggattcgtgg tgcttgacga
    10801 gaggcaggtc tcccgcatcc atgggaaggc attcgaggac cttgtccatg taccctcccc
    10861 gccacctgtc catgatgcag ttgtgcgaat cctctcagta ttcgaaccat caacagtccg
    10921 tgcgatgctc gcggagatgt atggaaagag gctgtcatac cgacagttct ttgaggcact
    10981 cgggtcggac agcgaccagg tgatacggac ctggaccagg gaagtcaaca cggtccgtga
    11041 aaacgggatc agggatttac ggaacgaccg tttgttcaat gctgcactca ccactctttt
    11101 ctctctcgct gaccggtatg agggtgctga tgacaaggct gtctgttttc tctgcgggat
    11161 ccggccgctc ctcgaggcaa tctcagggga ttccggcatt gaggcgttct gttctgccgc
    11221 caccgatctc ctcacaaagc gcccggggaa tgtcgggagc aggaaggtat ggaagggaaa
    11281 cgacctctca ctgttcaaag ctgcctataa agaactgaat gatatcctga agaggaagag
    11341 cagtctcttc cggatgacgg ttgaacctga tgatccgctt gtcactcgtg cgattgacct
    11401 tctgggagat ctctcgcttg tctatctccg ctacacggaa ctggttgagc gggaaaaggc
    11461 ggtccttggg ggacttgatt tcgaggacct gatcctgcac gcccggcagc tgttcctgac
    11521 caatgaagct ctcgttgcca cccatttcct gccacggttc aggtatatcc tcatcgacga
    11581 gttccaggac accgatccct cgcagtttga catcatcctc gcgatcgtcg gcaaacccgg
    11641 cccatctacg gactgcctct tcattgtcgg cgatcccaag cagtcgatct atctcttccg
    11701 ggatgccgat gtcacccgtt ttaaggcggt ccgggagatc attctcaatc ggtgccaggg
    11761 ccgtgaggtg caccttgata cgagtttccg gagcacaaaa gaggtgatag ggtgtgcaaa
    11821 ctacctcttc tcccggctct ttgcttcagc agagaaaccg tgggagttcg ggtacgagcc
    11881 gatccttgta tcggaagccc ggtctggaga tgcaggatca gtcgaactgc tgctcccccc
    11941 atcgggagag gattctgctg caacgaagtg gatcgaagct gatatggtag cacggaggat
    12001 ccagagcctt gtccagggat gtcatgtgca ggtgtatgag gaagcagagg accggtcgtt
    12061 tatttcgagg cctgcccggt acggcgatat tgcaatcctc cttgaacaga ggacgaacct
    12121 ttcctcgtat cttgctgccc tcgccagata cggtattcct ttctacgtgt acggggggac
    12181 gggattctat tcgcggcagg aggtatatga cctgtacaat ctcctccggt tcctccactt
    12241 caggcatgat gatatcagcc ttgcaggaac actccggtcc ccgtattgcg gcctcccgga
    12301 taccgatttg ttcctgatta gccgagagaa aggccggacc ttctgggaga agctccagaa
    12361 ctacggtgaa aagaccgggt cagagggtgc ggttcgtgcc tgccggctcc tctccctgtg
    12421 gagggcagat gcgggacgaa ctggcatcgt ccccctcatc aggcggatcc tttctgaatc
    12481 cgggatatac acggtgtatg cagcacttcc ggagggaaaa caggtcctgg ccaaccttga
    12541 gaagatcgtc gccatggcca gggttcggga gggtgccgga tcctacggac ttgccgactt
    12601 tatcgaggac ctcaggaccg ccatggatga ggaggagcgg gagggtgaag ccccgctgga
    12661 tgcccttgcc gagaactcgg tgaacgtcat gacggtccat gccgccaagg ggcttgaatt
    12721 tccgatcgtg gtggttcccg acatggggat gcagttccgg gagacgttcc cgccgatcat
    12781 gataggggat aacccgctcc tcgttgggat aacggtcccc gatccagagg aagagttcga
    12841 gcttacgaag actccgatgc ttcttgccct ccgggagctg cggcgccaga aggagcgtgc
    12901 cgagaagaaa cggctcctct acgttgccct tacccgtgca agggaccacc tgatcatgag
    12961 tgggacagcc ccgacacacc ctgacctttc gctatcgctg gcgacctccc ggatcgaatg
    13021 gatcttctcg gcgcttggca tcaccggcga tgcaattgct gcgggaggga tggacctcga
    13081 tgccagagac cagatggtcc gcctggccat aacgagcgat ccggcagcaa tccctgcaga
    13141 ggccgtagag atcaccccgg agcttcttgt cgtaccgaag gaatgtgcag gcaaaaacgg
    13201 tacctggtca ccgaaggagt ttgacaaggg tccggaaggg atcaggatcg tatcggtaac
    13261 ggacttggag aaggaacaat cctctcattc tcattcaccc accaaaaccg ttggaccccg
    13321 gtacctgccc ggtgttgacg gcactacgaa ggggactgtt atccatgaag tattccgtgg
    13381 ccgtgatgcc agggtggtgt gcctggaata cggggtgagt gatcctgaag caatccgaca
    13441 gtgcgaggag atggtatcgc ggttcctttc gtctccgctg atgcagcggg tgaagcgtga
    13501 gttctgcgag atgccgtttg ttatcaccta tcaggggaag catgtaacag ggaagatcga
    13561 ccggttatgt gaacttgtgg atggcacttg ggtggtaatc gattacaagt cggacccggt
    13621 tgatccttca gaatattcga aaaagacggg gaagtatcag aagtcaatgg aagtctattt
    13681 cgaagcggcc aggcagctgg taaagaggaa cgaagttgaa gcgtatttgt attttacgga
    13741 aacgggggaa ttttttaatg caaaaaatgg cttctgaaga ttaactggta tgcattaata
    13801 taaattccaa aataaattta aagaaaaaaa cgagacgaat ttcaaataga ggcagaaaat
    13861 gataatctca aaaatcaatt tacataacta tcggggtata cacgatataa atttcgagct
    13921 acaaaattat tcattatttg ttggtccgaa taattcagga aaaagtacgt tagttaatgc
    13981 tataagagct ttttatgaaa aagacggttt taaatataga aaggataccg atactccatt
    14041 aatccaccct aaagataagg aatcctggat tgaaattcat ttcaaattaa caaatgagga
    14101 aaatgaatca ttagcggaag aatataaaca aaataacaac cagttatttg tcagaaaata
    14161 ttttgataca gaaacgaaga ctcatgatgg taagtctgcg aatggaataa tttttgggta
    14221 taattccgag ggtatgctat cagatgagcc tttttacggg gcaaaaaatg ttcaaagtgg
    14281 aaaatttgga gatattattt atattcctgc ggttagtaaa gtggatgaac atactaaact
    14341 tacagggcct tcagcattac gagatctagt acaaaatata ttgtcatcta ctatcaccga
    14401 aagtgggaat tatgatgaat ttaaagaaca agtatcaatt ttttctgagg gtcttcggca
    14461 agaaaaaaca aaagaaggat attcaattca atcttttgag ggtgaattaa atgagtctct
    14521 aaatccatgg ggggtaaaat ttaaaataaa gttaaagact ccagctccac atgaaattgt
    14581 aaaagccatg cttgactatg attttgaaga tatgatgcat ggtaaaaccc aattaatcga
    14641 caattatgga tcaggtttcc agcgccattt tatatattct ctaattaaca taagttcgca
    14701 ttatactttc attaaggaaa aaaagaaatc caaagatttc tccccctcaa tgaccttaat
    14761 tttatttgag gaaccagaag catttctcca tccctctcaa caagacattc ttgcaagaaa
    14821 tcttatgaaa ttttctgatc cggatataaa tcaagtagta tgctctacac attcaccaca
    14881 tttcgtaagt agaaattcaa ataatattcc tgggttgatt catttcagta atgagaatgg
    14941 tataatcaaa acgtatcaaa taggtgatga tgaatggaaa agtatcgtag attcgaatca
    15001 aattgtgaat aaaatagctg ataaataccc aaaaatgaaa aagagactcc aggaagacga
    15061 tcttaaacct gaaatggaat cggtaaaaaa ttttttatgg ttaaatccag atcggtgtgg
    15121 aatttttttt gccaaccatg cagtacttgt agaaggacct tcagaggttg ctttaataaa
    15181 taaactaatt ggagataata aaattaaaaa atcaaattgc ggattgtatg tgatggattg
    15241 cattggtaag tataatattc atcgattcat gaatttgata tcatcattag gtataaagca
    15301 ctcggttatt tttgatgatg atgataatca ggaagaacat cttgaattca atcaactaat
    15361 tgaagaatcg cgcaatcctc tttttacata tgctataaag aaaattccaa aaaatcttga
    15421 gtcattttta tcgattccta atgcaggatc tgatcatcga aaaccccaac acatcttata
    15481 cctttacgaa tgtggaagta ttgcaaaaga taagatagat tcattctgcg gaatgattga
    15541 ttcatgtcta ccaaattagc attgcaaata atcgatccta tatgctcaaa aaaaatccct
    15601 cttaattggt gtatttttaa tttttgtgat aaatataagc tatttcacga tatccatagt
    15661 tgatcatgaa tgtttgttcg actcttgaat tattaatgtt ggatcatata atttttgatt
    15721 attcgattga aattccagtt tcgtcgtaat tataagattt gctaaatcta ctaatttcca
    15781 tccattcact ttccaatagt ttcctgtttc atttgaaata tcccaggcaa tcaataaatg
    15841 gcgtccatta gaatcaattt tcttcccact ggtgtaatag aaagatctga atgaactttt
    15901 ccaatcttga ctaactgctt ttgattctaa gaatgtaact ctattctgat tatcaacaat
    15961 tttaatgtct ggataaccag aggcagatag ttgaataggt ttcaatttag tctttttcag
    16021 ctcttcgaca aatacctctt ctattcttct tcccacttca ttgattcggc gaccttcaaa
    16081 ccgttgatgt gtcctatcaa catatttaag aaaattgttt gcagatgatt gaatttcagt
    16141 taacagttca agatcatgaa tattagtcag atcaagttgg taaacttcga aaggtgtcgt
    16201 tgcattcagt aggtctgaaa atgggatatt ttgtatcctc tttgatattt ttattccttc
    16261 tttaattaaa tcatgaattt cagccattaa tctgttttac aaaataatac catataaaga
    16321 agctagagaa ttttcttttg gttttcttta tcttttttct ttttcacctc aacagaaatc
    16381 cagtctcgtg caatcttcaa atattcttta tctatttcaa aaccgaaaaa ggctttatct
    16441 tctctaattg cagcgattgc agttgttcca attcccataa atggatctaa tactaattcg
    16501 gttttatcga taccatgatc tttaatgcac atctgaggta gacagactgg aaaaacgctg
    16561 gggtgaggtc tcgattcttt tattgtttcg tatgggataa accaggtatt tccacggtct
    16621 cttacatcac cagtttttga cttccatctc tggatattgc ttttatcctg ataaggtacc
    16681 ccgatggcta atttgtccaa tttggtattt cctgttttcg taaaatgaaa aataaattca
    16741 tgacattgat tgtgatatcg gacacttgta atgggtttat aatgacctac tgcaatgttg
    16801 cctttgatgt gggggtaatt ccccatggat tctcttggaa ttgcaattga ctttatccaa
    16861 tgtatcatgt tttgtaaaac aaaatgtcgg cgaaattgat tcgctacatc aatagctatc
    16921 catggatctg aagcagtatt tccaacgttc aagaaaaaag agccatcatc tttcataata
    16981 cgtttgcatg aaattgcgac ctgttctaac caggaaagat actcatctct tgattttgtg
    17041 tctttatatt catgatattt tattccgata ttatatgggg gagatgtcac tacaacatcc
    17101 acaatttttt catcaaagga ttgtaatccc ttcaaacaat ccttattata aattttattg
    17161 aactcgggct ttattttctt catagtcttt tctcaacatt cactgacgtg tttctgttaa
    17221 ttctcatatt catatgattt tccaattgtt ctcgaaaaaa aattctctgt tgtttagaat
    17281 aagtagtatc aaccgagtga agtgtgaaag tggaaaaaat taatttccgc tcatgatctc
    17341 cacccctctc ccaaaaaatt ttccgtaccg ctcgacctcc ctctccacag cctcctgttc
    17401 ctcatcggtg agttccttga acggattcaa gcgtacctcg atcgaatcct tcttcatcgt
    17461 cctcctccag gtcccggcaa cctttccgtg aaggacgatg acggcagtca tggcattccc
    17521 cttcgtgatc atccgctcga tctcccgggc atcgctgatt gcactccggt cggcataggc
    17581 gatcgcatac tcatcgtaaa ttgagaggag gagggcaacc ggtgggtcag ggatgacctc
    17641 cccgggatca ggtggatacc aatgtgtatt tccgttgatg gaggcccgct ccagctcctc
    17701 cctgatcgaa tcgagtccga cccccgcatc ccttctggag aggcctgacc accaggagaa
    17761 gtccttcagc tgggccgggc cgtggctggt aaagtatcgg tgtgcaagtt cggcaaggga
    17821 ttgttccctc gtgagtgctc tcgattccct gatccgttca tcggcaaggg cataggtatg
    17881 ctgcctcccc tgcatcggcc cgctgcagat caatccctcg agctctgcct gcatgatgat
    17941 gtgggcaagg cgctggacat cggtcacgat cccgatgtct gccagaatac ccttcaggtc
    18001 ctgtctcgtg agtacatggt gatcctccag ggccttttca atcgccgcat tgctcttcga
    18061 aaataaggtg ggatcaagcc ccagcttccg gttggatgct gccaccatcg atttcacccc
    18121 cggggctgtc agggcggtca tccacctgag gtcctcaggc aggatgaaat gccaggtgtg
    18181 acgtaacaca tgaacgcgga gaatcttccc atcgttatac gcatcctcga ttgtcgcagc
    18241 agttgcatcc ctgattctga gcccaagcga ccatatcgca gcggagaaat cctgggcctg
    18301 gaccgctccg gagtgtgcca ccgcctcagc cccggtcgtg aatggggagt ggctgaggcc
    18361 agaatgggag aggcggagac gtcggatctc ttccggggtc atcacaggag ggagctatcc
    18421 gcgcctggac tcttcattct tccgtccctt ccttcccggt agttccgcag aagcatatcg
    18481 ccaggccagt tcccgaccct cgaggagtgt atccatcctc cgatatatga aaagaatgtg
    18541 ccgtttccag tgacccaatc aaagtctacc tgggactggg acattctaca acgcgttggg
    18601 atcctcctgc agcatcccga agaggttccc ctccgtatcc cggcagtgag cccaccagcc
    18661 tatccctggt atccccattc gggggagtat gatgcttccc ccggctttcc tgacaaggtc
    18721 gaccgatcgg tcgagatcgg gaacctcgat ggtacaggta tatgcattga ccgcctggcc
    18781 ctcaaccggg gcaggacccc accggaagac gaggccaccg ttgatcccgg gatcggtctc
    18841 cggaccggtg atgataaacc agtaacggtt ctcatccttt atctcgatcc cgggtatcac
    18901 ccattcgcgg atctcccagc cgaacacatc gcggtaaaac tgtgccgccc gcccgggatc
    18961 atcggcctgg atctcgaaat gaataatcct gttcattgac ttcacaacct ctcttctatc
    19021 gatctcctct ccagcagatc catgaaagac cggtgacttg atctccggat actgatctca
    19081 tccctctctc cattttttct cgaccagttc tgcaaggcga tcgatggtct ccatccagcc
    19141 ctctgccatc gccgatttca acatttcgtc ctcaacgtct tccggagtct cgataacgga
    19201 tttgtcgacc atcttcgtct tcccgtctac ctcttcgaag gtgatgacct cgagctggac
    19261 atggccgggc atcccctcga actcgagggt ctggacgatc cgtctcgggg agacttcgtg
    19321 atacacccca tggaaggcat actcgttgcc atcggcatca cgctggatga acctccagct
    19381 ccctccgctc ctcgcatcca tcgtatcgac gatggtcgtg agatttcccg ggccccacca
    19441 gagcgggatc ctggcaggat cggttactgc cgagaagagg cgttcaggag gggcatcgaa
    19501 aattcttgtg agggaggccg gttttccagg ctctatcacg aggacattct tttccctgtg
    19561 ctgtgtctcg tcatggagta gtgtttcgag cttgtcaagt gattcgttcc accccgcttt
    19621 tgcctgttcg ctcggctcgc caggaggcat cccttcatga acgagcgtga acgttgtttt
    19681 gccgttcttc tcctcgaacg tcagggtcac gagcagctcc agaggccaca ctccggtcat
    19741 cccgtagaac gatgccggaa cgatcgctcc tttctcatct gaaaagctgt cggtggtgac
    19801 gatccttttt tccgggacaa tctcccggta gattccggta ctccagaatt cctgcccatc
    19861 aggggatcgc atgcacaaga gggatgttcc ccctaccctg aaatcgatca tgatgaacgg
    19921 agccgtgaag tcctttggcc cccaccagcg ctttacatac cctgggtcag tccatgcctt
    19981 ccatacgagg tctaccgggg catcaaagac gcgggtgatt gagaggccgg cactctttct
    20041 ctttgtcatt gttttctgca tggaattcct ccattcgttc ccgcggtctt gttattcccc
    20101 gttattaaaa acttaaccgg gagtttcaaa atctgccaat cctccggaag attctgtacc
    20161 aggatcaaca gaacagagaa aaagtgctga atcggtattt tccctgtgag accgggggat
    20221 tcctgcccgg tccctggatc ttttacaggg acagagttaa aaaagggaat gttcgcacag
    20281 gatattcccc tcatcagatc cattcaactt cccgggcggc cggatacttc agaatcactt
    20341 cagattgaac cggaaataat tgtccagact catgaatacc cctttccact ctctgcttcc
    20401 tgtaaaaccc tgccgtaccg gaaggagtca cccgttgtag tagtgtcgat cgaacgtctg
    20461 gcggaggaac tctttcttcc tgtgatcgct cccctttacc ggtggcttga ggtacccgac
    20521 ctccggggtc tggagtgccg ggcagaacgg gcagagggcg gagtcgacat catttgcctt
    20581 gtcccggacg gggcagtaat atgtaccgtc gatgatctcg accttatccc cgccgggaaa
    20641 cggtgtcccg accgggtggc cggggatctc ctgcacgagc atgcagaagc cggagaggag
    20701 gaatttcagg aaccggagcc ggacctgttc ctcgtctccc gaacggcact ggcatgtgac
    20761 catcgtccag tattcttctg tctttttctc caatggctgg ttcatcggag cgaaactccc
    20821 ctgctgcttc atcagccgga gtgcctggta ggtcccgtgg aggtgggctg tgatagtctc
    20881 ttccaacttt ttccggtatt ccgggttgat atcctggatc gcacccgaga agttccaccg
    20941 catctgctgg agatcctttg gggagtagga gaggacgatg ccggcgatgg cctggccgag
    21001 ttcgccacga gtcttcatcg agccgagcaa taagcattgt ctggttactt cttcccggtc
    21061 catggtcagg gtctccgcag atctgtgtgg atcatgcctt ttctgttagg tatgctgtca
    21121 caggatttca ggtttcagat ccccttatga aatcgtaagg ccacacagcg ggtttcaatt
    21181 tttctatcaa ccggtgcaca ggaatgatgt ggtaacgcgg agtgggcaca tcagcctggg
    21241 acactgctgg aatgaaatag gagaacgatc agagaacgaa atatgggcaa caccattgac
    21301 cgctaccgat gtactgtatg cagctacctc tatgatccag cggtgggaga tcccgggtca
    21361 ggaattcccg ccggagtgtt gtttgaagat ctccccgagg acagagtctg ccctgaatgc
    21421 ggggtcggca aggaccagtt cgagagagtg aaagaatgat ccgggtaaaa cgcatctatg
    21481 atgttcccgg ggagggagat ggctttcgcg tcctcgtcga acggttgtgg ccgagaggca
    21541 tgcggaagga gcaggccggg atcgacctct ggatacggga tgtgggagca agtcccgcac
    21601 tccggaaatg gttcggccat gacccggcga aatgggagga gttccaggaa aagtacttcc
    21661 acgaattgtc cggaaagcct gaactcggcg aacttctcac tgcacttggc cgggatcatc
    21721 ctgtgatcac ctttctcttt gcagcaaagg atgaggagca caataatgct gttgcactga
    21781 aaaaatatct ggacatgaga ggcgaactgg gatgaaagtc atcatcgacc ggctcaattg
    21841 tgtcagttgt gggacctgct gggagctctg cccggatttt tttgaagaga accccgacga
    21901 ttccttcagc cagatcagag agaaattccg cctcgacggt ttgaatggag aggggatccc
    21961 ctccggggaa ctggagccgt gcgtggtcga tgctgctgat ctctgccctg catcggtaat
    22021 ctcgatcaaa gagtgatacg accaggggag tagtgcttga tccctcagta agaaagcgtt
    22081 caaagcgttg atcggggaaa tctgcttagc ggagattact atggatgact ggcgtgggtt
    22141 gacctacccc gggatgaatt acgattcgaa ggaaagtgca gcctgaacag gggtatcccc
    22201 ttttttccaa tccaagacag ggtatcatcg aagagaggaa gcttgctgta gcccctcaca
    22261 aagaagatgc cctttgagtc attgaatttt taaagatcca cccctataat gaacccctcc
    22321 tccacccttg agaaccgcca atcatcggtg ggataagagc ccggcataca tgcctcccat
    22381 gggtacatga tggagaaccc ccggatacca acaagaggga acgctccctg ccggaaatgt
    22441 aacgtttatt ttacattaat atcgaatacg tacgagacat ctcatggaat taccccgctg
    22501 cctgaaacgt ggacttgccg gtattatcgg atctgcaggt ctagcatacc cggtctgtgc
    22561 ggctatctgc ccgaggggaa aggggtactg cccttacccg ggccgctgtt tcttgtatac
    22621 ggatgtcgat accaacgcca tttgcgatta taccccccgt tcggtcacca ccgccccgac
    22681 ggcagccagc cccactcctg ctgcctcccc ggtcgtggag gcgacctcct ccttatcaac
    22741 cccggtcacc tcatctgtca cacccgctgc aacgggcccc gcctctgtca caccggcagc
    22801 aacggtctcc tcaaatgcct ccgttccgtt caccccggat gcaggattct tcgtcccgga
    22861 tggcctgatc atgggagcgg tggcaaccat ctttctcgtt ctcctgtttt tcttcctctt
    22921 caggtcaggg atccttgggt ccagactcca aaaacgcgtt cctgctctcg gggcgtcggc
    22981 attgttcgcc cttgggatcg gggagatggt cacttatctc ctgctcggag aggagacgct
    23041 ggcgtcggca ttctctgtca tctacctgct tttcgggact attcttgcag catgggtctg
    23101 gaagagcggg gctatgtcgc gaaagctcgc ggtcgcaaca gtgatgataa gtgctctctt
    23161 tggctttctc ctcctcgccc cgcttatgcc gatggagttt cccgggatcg tgagcatcgc
    23221 gacctccggc cagaccctgg ccccggggat catcgggatc ctggcaggga tcgggctcgt
    23281 tctcctcgtc gggagaacct tctgcggcca tctctgcccg gtcggatcgg tacaggagct
    23341 cgcctggaac gtgccgggga agaagatcga tatcaggaaa acgcggtatc ttgaggtatt
    23401 ccgtgccggt gttttcgcgg caaccgtgat cggagccctt tactatgtaa acctgatgga
    23461 gtacaccggg atctacgatt tcttctcgct gaccctctcg ataggacttg ttgtattttt
    23521 cgtgctgctc ctcctttcca tatttgtata ccggccggtc tgccgtggga tctgcccgtt
    23581 cggcctgctc ttctccatcc ctgcccactt cagcctcttc cgcctgcgga ggacgagtgc
    23641 ctgcatcagc tgcaaaaagt gcgagaaggc ctgcccggcc catgttgccg ggaaggattc
    23701 ctcgaagcgg gagtgctacc tctgcgcccg gtgcacggct gcctgcccag tacagggagc
    23761 cctggtctat aaaaggtgag atttcaatcc acggctgcca ctcccgtttg aagaacacga
    23821 taaaggaccg ggtgacctgg tcaaaggaac caggtgacct ggtcagccag tccctcctcc
    23881 tacccggcca tgagtgctga taacacattt cacccgtcgg ctgagcctgg atacaaacaa
    23941 cggatgagtc atccgggtat tcaagtcggt atcagcagga cataccggat ggtaccctct
    24001 tccatcccgg ctccttgagg gcaggggagt ttcatgtttt cgctatccat atactctgat
    24061 gatcgatatg accaccataa ccgatgaatt catgagtgag atgatcggaa ggacgaaaga
    24121 ctacagtatc gtgctgctgc ggatcggacc gaaccggtac atggccgggg tggaagcgat
    24181 tatctgggag catgcacgga gaaatttctc tctgcgatct tcgggaaaac tcccaattgt
    24241 ctgcccggtc aatgacggca gcgagctctg tggtgttggg atattcgcaa cgggtcctga
    24301 ggaaacccgg aatatcatga acgaggaccc tggggtgatg aaagggatat tctcgtacga
    24361 actccatccc tgccggagtt ttcccggtga ccgtctgccc gggcaactgg ggtgaagttc
    24421 attccacagg agcaccatcc agtccatccg cccgcacctc cgggttcgta catcgaaacg
    24481 gcagagtcaa agaacctccc accaagcccc ggcaggattc accgacagag tgatattcga
    24541 ggaaaagtgt tggttgggca aggaaggagg tggtacaaga caatcacaaa accccccggc
    24601 agatcaataa gcccgccaat atcaacactg ctcttctacc tccaaaaaaa tgctggtggt
    24661 cgatctgaac tgcagtaccc agctcattga atagcccctt gaggaatttt cctttggaga
    24721 atcctgatgg aatacgtttt tcttcgggaa tacatagttt attgagttga tttgttaaca
    24781 ccattgcctt gcaaattatt atcatccctc aatagatccc ccttcacatc tgccaccata
    24841 ttacctctgg gtttttacgt cggttgtaat gatgacagat cgtgatgatg agaggtaacg
    24901 ctctcctcac cagtcaaaca tttcatggat attccaggag ggaaaagaaa gatgaagatt
    24961 gatcatttct ttacgatgct tctgctcctt attgcatttc tcatgctcat agggaacgtg
    25021 cagggcatct cccaggatga cctgaacaac attgttatca gggatgtatt gaatgagaaa
    25081 atggaaggga tgtgggtgta tgccaagccg gaaccggttg aggctggcag tacgatcacc
    25141 acatggtatg gtgacatact tctccctgag aaggagggat ggctcttctt catcgatgat
    25201 gctccgatgc agagctgggc gcacccatgt cggtacatct tcgttgatga agccggttct
    25261 gtgaccgtca gggacgcgct ggggcccccg atggatcttg cgaagtggaa gaaagtggca
    25321 ggcgttgcga cacccctaac gtccccccta tcagccatgg ttccgtcagc gatccgcgga
    25381 agcatcaatg ctctgccgcc ctgcatgaac cccggtcact gttatgcggt tatcatcagc
    25441 ggtggggcag actcctacaa taactgggag cgctattacg gggatgtatc gttcatgtac
    25501 aagaccctcg tgaatgatta tgggtatctg gacgaccaca tcatcgtatt gatgtcggac
    25561 ggaactgacg ccggtcttga ccagcataaa tatgacgata cctatgtgaa ctcaaaccct
    25621 gatcttgatg gtgacgggga cgatgatgta gggttctcgg ctacgaatgc gaacatcggc
    25681 acggtctttg acgatctcca gacacgactg gaaaacagcg accacctttt cattttcacc
    25741 accgaccatg gcggccccga aaatgaccct caagtgggca ctgacgtcat tatcaatcta
    25801 tggtacgaga caatgaggga cgaccagttt gacgccaatc tggataagat caccgcgacc
    25861 gttcccatca tgatcaccat ggaacagtgc tacagcggcg ggttcatcga tgacgtgatc
    25921 cccgggcctg ttggccagga gcgggtcatt gcgaccgcgg caaacgcgtt tgaatcttct
    25981 tggggtgata cttttagcac tctctggatt tctgcggtgg caggccatga caagggtggc
    26041 gcatcggtcg atgctgatac caaca
//
LOCUS       DAOOCO010000008        24734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_1692_length_24734_cov_5.134122, whole genome shotgun sequence.
ACCESSION   DAOOCO010000008 DAOOCO010000000
VERSION     DAOOCO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1692_length_24734_cov_5.134122"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2107)
                     /locus_tag="PK154_00995"
     CDS             complement(<1..2107)
                     /locus_tag="PK154_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPW09671.1"
                     /translation="MSQNQGGIPELLAPAGSMDALVAAVNAGADAVYLGGKRFGARKY
                     SPNFNDEELGQALAYAHMRGTRVYVTVNTLVHDRELPEVARYLGDLYAMGADAVLIQD
                     PGIALIARDIVPDLPRHASTQCTITSREGVLHAHREGFERVVLAREVPLAELDRIFQV
                     PPDKRPGIEIFIHGALCYAYSGQCLLSSVIGGRSGNRGACAQPCRRDYSLVAGTPDSF
                     GRITRPRRVPLGEPCLLSTKDLCLYPHLAGILERPITALKIEGRMRSPDYVAIVVSIY
                     RKALNAVAAGTFVPEQSDMDDLAIAFSRGFTSGYLCGDRGPSLMGRDRPDNRGFFIGT
                     VRSVRGSETRVAAAGSRALSAGDGLVGIDPVTRAESGFVLRERPLRAGRDLLIRQPTG
                     CREGMVLYLTRSVSLEGRAGAIRSAAGPAGRFPIPIEVALSVATHKPPVLSGSFKGPG
                     GGVVRVTTEADFIPGRAEERSTTSDEISRQIRKSGGTAFSVSDLSLTYGGGLFLPVGA
                     LNRFRRHFFSEAERALLQTYLPDNRKSGEARTRLAMRLSTLDRPLKRGSRNPELAIIC
                     TDVDSVKAACQAGCKRVYFEPDPGDMGRVLREAIATCRESKVRIAWKWPRIPLPEFTT
                     AALPLLPGLADAGLEEVMTEGAMYADPIRDRAPGIRVTGGPDLNVFNACAARALAHDC
                     PGVTLSPELSGEDIALLCNR"
     gene            2208..2837
                     /locus_tag="PK154_01000"
     CDS             2208..2837
                     /locus_tag="PK154_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPW09672.1"
                     /translation="MKCVASVSDPRCIPEAEEAGADLVELRLDLFPPTLSQEQKNCVK
                     RCTLPLIITVRSRQEGGYFRGSPDDWWRLVLPWLSDATLVDVEQDFSMLTPLINDAGA
                     EVIASFHSGRMLIGGELGAIATRLRAYGTPKVVVRPESPIDVLTLCWFTHRTEKPVIT
                     SVIGSQYRYARVMLPLFGSEMAFGYAGQPTAEGQFHIREMRQMVETLIE"
     gene            complement(2871..3881)
                     /gene="aroC"
                     /locus_tag="PK154_01005"
     CDS             complement(2871..3881)
                     /gene="aroC"
                     /locus_tag="PK154_01005"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW09673.1"
                     /translation="MNTFGRSFRITTFGESHGPVIGVVIDGCPAGLPLSVSDIQPLLD
                     RRRPGTSLLTSPRAEPDRVEILSGVFEGKTTGMPVALLVRNADARSSDYDTLREVYRP
                     SHADYTYQAKYGIRDHRGGGRSSGRETVGRVAAGAVAAKFLARAGIEISAKVEEIHGV
                     SDPGAMEEEIRNAKQSGDSVGGIIRISATGVPPGLGDPVFGKLDAQIAGAMMGISAVK
                     GVEIGDGFASARLKGSEHNDEMDATGFLSNHSGGILGGISNGNEIMVRIAVKPTASIA
                     LPQKTRSVSGDGQTIALTGRHDPCIVPRLMPVAEAMLALVLFDAWLEQEKYRADIRKN
                     GL"
     gene            complement(3878..5263)
                     /gene="aroE"
                     /locus_tag="PK154_01010"
     CDS             complement(3878..5263)
                     /gene="aroE"
                     /locus_tag="PK154_01010"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW09674.1"
                     /translation="MRVVLIGFRGTGKTETGRLLSRLTSVPFLDTDALIEASAGMTIH
                     DIFRREGEEGFRAREREVIRALPPGEAVIGTGGGAILDPANVAALRKGSTMVLLEADE
                     VVIEKRIAASTRPPLTTLPLREEIHELLELRRPHYAAAADFRIDTSLKSPNEVVLAIR
                     KFLHDGTVPLQARKNLVQFLAGSGIPESEVAEARILTDVDLWDPLTRLYGIVGCPAIQ
                     SRSPALFNRLFRQYGITSHYLRFQDPDLLRILRLAEEIDIRGLSVTIPFKQEIIPTLT
                     DVEVHADAIGAVNTVVWCGSRCFGYNTDWIGIREPLSDHRGEKAVILGAGGAAAAAVY
                     ALQSLDMEVTILNRTVEKAASLAERFDCAYGPSSAFDRLNPAVVVNATSIGMEPELKS
                     PLQKHQLRSSMTVFDLVYTPPETPLLRIARNSGCQTIPGTEMFVRQAAAQFRHFTGIA
                     PSLELVRGAME"
     gene            complement(5260..6531)
                     /gene="aroA"
                     /locus_tag="PK154_01015"
     CDS             complement(5260..6531)
                     /gene="aroA"
                     /locus_tag="PK154_01015"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW09675.1"
                     /translation="MSVTLERSGPVDLTLKAPASKSYTHRALVTAGLARGTSTLTRVL
                     RSDDTRITASALQQMGVSLQWEGEQIRVTGTGGVLQCGDHSVLDMGDSGTSLRILTSV
                     ALLCQRPVIITGSPRMKERPVGGLVNALNSLGGRIQYLENAGFPPLMVDGTLHGGSVK
                     VDSTVSSQFASSLLLSAPYAASPLELQLLPGAVSRSYLDVTVDVMKVFGVPVVREGQD
                     HFVVHPGKGYCGRDYQVEGDYSSASYFFAIAAVCGGRARVSSLSPGSVQGDRQFLDAL
                     AEMGCRVSCSDDCITVESDGVLEGIERDLSSSPDTVQTLCMVAACARSPSWFSGISHL
                     QFKESNRIFAILSTLQQLGGDVTAGKDGSITIRPAPLHGGTIDPAGDHRTAMSAAVLG
                     LAVGGITITDAGCVSKSFPEFWDILQRDGLV"
     gene            complement(6528..7319)
                     /locus_tag="PK154_01020"
     CDS             complement(6528..7319)
                     /locus_tag="PK154_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPW09676.1"
                     /translation="MTLIVLGPEGTFSHELAVRVYGGEILLAPTIHEIFSMVEQGLGD
                     GLVPIENSEAGGVGATLDGLLQHQVYITAELYMPVHHHLAGFTPLDSVTVIYAHPQTH
                     EQCSRITNELKIPVIHTSSNAASARELAGSPGSAAIVSRMTADLYKIPLLRGNVENNP
                     GNTTRFIRIARTKGKEKGSTKCSILVDPEKDQPGLLYQILGVFAERGINLTRIESRPS
                     KRRMGEYLFFIDLVYLPGCHDALVALREKSRFRDLGCYQEVKVPE"
     gene            complement(7316..8140)
                     /locus_tag="PK154_01025"
     CDS             complement(7316..8140)
                     /locus_tag="PK154_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPW09677.1"
                     /translation="MKVGIIGGTGKMGSLFAGVFSRAGHEVQVCGRGCLSLCLDLAVS
                     SDVLMVSVPIRDTVHVIQDITPFLSKEQILCDLTSLKTSPVAAMLASRASVVGLHPMF
                     GPTVPSLNGQTIIATPARIDDAALTSILSVFEREGARITLSSPEEHDRMMAVVQGLTH
                     SITLTVAETMRRLGVSPEATGPYRSPVYQIEMGLVGRLLSQDPDLYADMLTENPYLPP
                     VLAACEASFAHIREAIESGEPDPFREIFTHDAEFFAGITQSAAEETDYLITAMVQR"
     gene            complement(8145..9131)
                     /locus_tag="PK154_01030"
     CDS             complement(8145..9131)
                     /locus_tag="PK154_01030"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HPW09678.1"
                     /translation="MKEFWVDIRPFRKELVTTALESGADVVIAPSAPPVKALGRVSVV
                     SDDGDLVWGTDVFEVRIEGGTSLAEAEALAEKGYVIVETGDWRVIPLENLVALSDRII
                     AAVRSTEEAEVALTVLEKGVCGVLVRDADPALIRAIGTMVKAGAGAVDLVPFTITAIR
                     PIGMGDRVCVDTVSMLSDGEGILVGNTSSALLLVQAETLENPYVNPRPFRVNAGAVHA
                     YTLVSGGKTAYLSDLKAGDHVLAVTHEGSIRDAGVGRVKIEHRPLLLVEAEAGEVKAS
                     LVLQNAETVRLVGEDGIPVSVVALAPGDRVLGRLLQAGRHFGIAVRERIIEK"
     gene            complement(9128..9913)
                     /locus_tag="PK154_01035"
     CDS             complement(9128..9913)
                     /locus_tag="PK154_01035"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HPW09679.1"
                     /translation="MIGKEIRIERIMNRNTGRSVIVPMDHGFTLGQIEGLTDMTKTIS
                     EVSDGGANAIVLHKGIVKRGHRRRGRDIGLIVHLSGSTSLNPDPNDKVLVCTVEEAIA
                     LGADAVSIHINLGAPNESKMLEEAAIVVKECNRWGMPLLVMIYPRGKGINPYAPSTVG
                     HCVRVAEELGADLIKTNYTGDPASFAKIVAACSVPVFIAGGEKAGDRETLVAIRDAVK
                     AGGAGVCVGRNAFQRDNPRSFVEALCKVVHDEVDPDEALKGCR"
     gene            complement(9917..10708)
                     /locus_tag="PK154_01040"
     CDS             complement(9917..10708)
                     /locus_tag="PK154_01040"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HPW09680.1"
                     /translation="MRGKEIRLERIIDRNTRKTIIVPMDHGVTNGPVAGLIDLGEAVD
                     QVAEGGANAVLGHVGLALHGHRKSGRDIGLILHLSASTAIGPDPNEKVLVNSVTNALK
                     MGADAVSVHINIGADSEARMLADLGSVAIECMEWGMPLLAMMYPRGRKIKDEHQVDHV
                     ALAARVAAELGADMVKTVYTGDPDSFRTVTRGCPVPIVVAGGSKTDDRSTMEMIEGAM
                     QGGAAGISIGRNAFQHRTPARFVRAAGMIVHEGKTAEDAWEFLKG"
     gene            complement(11189..11680)
                     /locus_tag="PK154_01045"
     CDS             complement(11189..11680)
                     /locus_tag="PK154_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HPW09681.1"
                     /translation="MLCEMCGAAIHGPPKTVRIEGAELDVCSQCAKYGTEVQKQKKPE
                     PRKGGPAARTVATPVRKTRDVLDLIEGEIVDDYGERIRKARMERGLSQKDLAMELKEK
                     ELLIKKIEKGDLIPEDDVRIRLERALSIRLIDTPEISEEKKKQGKVVPTLGDVISIKK
                     VQK"
     gene            11989..13227
                     /locus_tag="PK154_01050"
     CDS             11989..13227
                     /locus_tag="PK154_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HPW09682.1"
                     /translation="MAESARQPQEPQSPDEKSRFLLDRVNTLESRNLELREQLRQIET
                     DKRYIETQKIRYEREVRKLKSEIEQLRSPPLVIGTVTDLVDSNRVIVRSSAGPRFLVR
                     SSMCIDPDQLKPGVRCTLNQQSLAVIDILPESYDAAVYGMEVVETPDESYADIGGLDR
                     QIMEIKEAVELPLKRPELFDRIGIDPPKGVLLHGPPGTGKTLLAKAVAHETNAYFLRV
                     VGSELVQKYIGEGARLVRELFELAKKKSPTIIFIDEIDAVGASRTEATTSGDREVQRT
                     LMQLLAGMDGFERRGDVKIIGATNRIDILDRALLRPGRFDRIIEIPLPDVEGRLAILN
                     VHTRKMTLDEEVNLREIAFLTDGKNGADLRAICMEAGMFAIREEHDSITRTDFLNAIE
                     KIGLDFDRQKFASSFGAMFA"
     gene            13478..14092
                     /locus_tag="PK154_01055"
     CDS             13478..14092
                     /locus_tag="PK154_01055"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HPW09683.1"
                     /translation="MRPDYQNVAGVLLFLAGFIALMGIITGEIFYPAGYSTAHSEISD
                     LGSTRPPEALIFQPSATIFNTTMILAGILIITGAYCASRSGWERWSSLLLGLLGVGIL
                     GVGIFPGNVAVLHPLFAMFTFVIGGIAAVLSSRGLPGPYRYLSVILGAITLLSLLLMG
                     ILIPVLGDGGTERFVAYPLIFWLTGLGAYFLGRASMQQQAPHPA"
     gene            complement(14288..14914)
                     /locus_tag="PK154_01060"
     CDS             complement(14288..14914)
                     /locus_tag="PK154_01060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW09684.1"
                     /translation="MVRTTAPERADTADKKRKESHHHHEVFSAEKAGQLDTRLRRLLY
                     RPDILAERLVKPGDHVLDFGCGPGFFTREFANQVGDGGKVIAVDLQEGMLRILREKME
                     REGLMKRVMIHQCTPDSIGLPPAFDGTVDVACAIFVVHEVPDPTRLFWEISRLLKPGG
                     LFFYTEPFYVVPGREFREHLKDAENAGFEVVKRHLFFLNRAAHLRKAV"
     gene            complement(15211..15897)
                     /locus_tag="PK154_01065"
     CDS             complement(15211..15897)
                     /locus_tag="PK154_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW09685.1"
                     /translation="MPHHTGAILQRDGKTFAVVTRVPAGVVNPEALENLAAVGRKYKV
                     PMMKLTSGQRFMLIGIPEKDLASLYKDLGPLAEKETAPCVKYVQICLGTDVCKYGVQD
                     STGLGLELEKKYHQEEFPAKIKFGVSGCLRCCGESYTRDVGLIGTTKGWSVVFGGNSG
                     RRPALGTLIAADLSKSDALDLVDRLLEYYREQAHPKERTARFLERIGFERLKSDLLTL
                     LPYIRLSEVR"
     gene            complement(15913..16251)
                     /locus_tag="PK154_01070"
     CDS             complement(15913..16251)
                     /locus_tag="PK154_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW09686.1"
                     /translation="MKIIDAITATSSPNPHHVDARKLCEIPHAVVVMITLQPGESLKR
                     HKTPVDVIFYVLEGTGTVEIGEEQATVGKDTLIESPARIPHRWINESGEVFRVLVIKV
                     PKPTEETKLL"
     gene            complement(16355..16714)
                     /locus_tag="PK154_01075"
     CDS             complement(16355..16714)
                     /locus_tag="PK154_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW09687.1"
                     /translation="MQDSCTVSQTVRYLSKRWTLLVILELYKGEGYTRRFSELRDALP
                     GITPKVLSERLKELEEEALISRKVDATAFPVRTEYTLTESGLEIIGIIRAIKQWALKW
                     KIENIACGNQDCKVCVI"
     gene            16800..18419
                     /gene="hcp"
                     /locus_tag="PK154_01080"
                     /gene_synonym="priS"
     CDS             16800..18419
                     /gene="hcp"
                     /locus_tag="PK154_01080"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPW09688.1"
                     /translation="MFCYQCEETVKGIGCTVKGVCGKEDEIAAYQDVLVFLCKGLAAR
                     NLAAIKKGKGNPETGLFIAEALFATLTNVNFDKDRFTAMIKKAVALRDALPRSSSTEP
                     DACTWKPGSESDILKKAETAGILATENEDVRSLRSTLLFGLKGISAYYTHAEVLGRTD
                     DSITTFIQKALASMLQDLPVPDMVSLVLECGGVGVKTLALLDEANTSAYGTPAITAVK
                     TTVRNRPGILITGHDLKDLEMLLEQSKDAGVDVYTHGEMLPAHAYPAFKNYPHLVGNY
                     GSSWWHQKEEFDQFNGPVLFTTNCLVPPKENYKGRLFTTGLTGYPGVPHIEAGPNGTK
                     DFSPVIALAKTCPAPNALPGSGRDLLTGCAHDAMLSLADKVVGAVKNGQIRRFVVMAG
                     CDGRQKEREYYTEFAQALPKDTIILTAGCAKYRYNSLDLGKVDGIPRVLDAGQCNDCY
                     SLVVIAQALAQAFGVGINDLPVSYNIAWYEQKAVLVLLALLHLGVKDITLGPKLPAFI
                     SPAVLDVLVKNFGIRKNSSVEEDLPRMVPAL"
     gene            18476..18841
                     /locus_tag="PK154_01085"
     CDS             18476..18841
                     /locus_tag="PK154_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW09689.1"
                     /translation="MAEFDLEKAEKRDEKRAELRGKVLRLKDLVEYQDGTVASRMIVN
                     RKVGSITLFSFDEDEGLSEHTAPYDAVVTILDGKCEVWVSGTTYQMKEGETIIFPANA
                     PHALSAITRFKMMLTMIRG"
     gene            18844..19125
                     /locus_tag="PK154_01090"
     CDS             18844..19125
                     /locus_tag="PK154_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HPW09690.1"
                     /translation="MAGDEKDGYYCSICGGIPPDKIKTRRILIDGKETGIDHLEFILE
                     EVKKLHLTDESEIVAEIMKRVRVFNYVPTKKADRYAEALLREYRQQETP"
     gene            19126..19371
                     /locus_tag="PK154_01095"
     CDS             19126..19371
                     /locus_tag="PK154_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09691.1"
                     /translation="MLDRCPGAATLRTPTLTIRKCPECGEEIEVFSNDVAVSCSRCGF
                     TVYNDALSCVQWCKYAKECVGDETYRRLTSHHPKEEG"
     gene            19573..20430
                     /locus_tag="PK154_01100"
     CDS             19573..20430
                     /locus_tag="PK154_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW09692.1"
                     /translation="MNRKIIDIDQKKCTGCGQCIPDCPEGALQLIDGKARLVSDLFCD
                     GLGACMGTCPEGAITVIEREAGEYDEKAVMANVVPQGPAVIKAHLEHLVGHGEKKLYD
                     LAIEYLREHQIPVPSHRAPGSPPDAGVCGHPSFAGCPGSAARTIPRRPREGYRHAPGE
                     TGSELRQWPVQLKLLNPEAHYFDNADLLIAADCVPFAYAGFHQDFLKDGIVIIFCPKL
                     DTDIEDYITKLKEIFSLHQIRSVTILHMEVPCCSGVAYIVDQALKGSGKTIPVREYTV
                     MIDGGVVES"
     gene            20587..20709
                     /locus_tag="PK154_01105"
     CDS             20587..20709
                     /locus_tag="PK154_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09693.1"
                     /translation="MNTVRSTSEDTSGGHMYDRCQRSSPALQEPRRQPTHLTHY"
     gene            20830..21747
                     /locus_tag="PK154_01110"
     CDS             20830..21747
                     /locus_tag="PK154_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09694.1"
                     /translation="MNAIEVEHLTKRFGTVTAVDDISLSVQNGGIFGFLGPNGAGKTT
                     TIRLITGVLTPDAGSARIRGIDIHRHPIDAKMQMGVIPESSTVYGDLSAEQNLHLTGK
                     MYGMPRPIREERVDELLTKMGLLAQRKYPVRTFSKGMKQRVSIACAIMHRPAVLFLDE
                     PTSGLDVQSRRLVVETIHQMNEEGSTVFLTTHNIEEANTLCRTVCIINRGRIVAQDSP
                     ERLRRMFEATQSVEVSFSRGVRNDFFQAEGIIRAEPCGDKWRIYTDNPDRVVKYLVSR
                     ADRESLGITSLAITGPSLEETFVQLTGCT"
     gene            21744..22493
                     /locus_tag="PK154_01115"
     CDS             21744..22493
                     /locus_tag="PK154_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW09695.1"
                     /translation="MKTPVVFLRGILTICEKNIFLYYTKPPVLMFGILFPIFMFLAFF
                     VGRNLDLVLFFPGFLAMMLFFTASSVGPLITPWEKREKTYERLLSYPVIPESIIIGDI
                     LAGAIFGLGISLIIWIVSTLFIPMHIINPALFLFTFVLGTIACSSLGALLASPASNSP
                     PNVMIFSNLIRFPLIFISGIFIPLSQMQGLSLALASCSPLTYVVDLFNAAISGGSFFS
                     PLLDGGVLIAATVLFILLARFIQVRNLVKGL"
     gene            22959..>24734
                     /gene="katG"
                     /locus_tag="PK154_01120"
     CDS             22959..>24734
                     /gene="katG"
                     /locus_tag="PK154_01120"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPW09696.1"
                     /translation="MNQGPTGRGRSIRDWWPNQVNLKMLHQHSPLSNPMGREFNYAEE
                     FKTLDLAAVKSDLRALMTESQDWWPADFGHYGPLFIRMAWHSAGTYRVGDGRGGAGSA
                     QQRFPPLNSWPDNANLDKARRLLWPIKQKYGRKISWADLLILAGNVALESMGFRTFGF
                     AGGREDAWEPDESVYWGAENEWLGDKRYSGDRKLENPLAAVQMGLIYVNPEGPNGNPD
                     PVAAARDIREVFARMAMNDEETVALIAGGHAFGKTHGAGPASHVGPEPDAAPIEEQGL
                     GWKSSYGTGKGNDAITGGPEVIWTQTPIKWSNNFFRNLFTFEWELEKSPAGAYQWKPK
                     GGAGAGSVPDPHDPSKRRAPGMLTTDLALRFDPVYEKISRRFYEHPEQMADAFARAWF
                     KLTHRDMGPRSRYLGPEVPAEELIWQDPIPAVDHPLIDAKDIASLKAMILATGLSVSA
                     LVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPARLAKVLGTLEGIQREFNS
                     KATGGKKVSLADLIVLAGCAGVEQAAKNAGHSVTVPFTPGRMDASREQTDVDSFAVLE
                     PKADGFRNYLKGKYSVSAEELLVDKA"
BASE COUNT         5282 a         7359 c         7071 g         5022 t
ORIGIN      
        1 gacggttaca gagaagggcg atgtcctccc cggacagctc cggggagagc gtgacaccgg
       61 gacagtcatg ggcaagcgct ctcgccgcac acgcattgaa tacgttcaga tccggaccgc
      121 cggtcacccg tatcccggga gctctgtccc ggatgggatc ggcatacatg gcaccttctg
      181 tcatcacctc ttccagaccg gcatcagcga ggcccggcag cagggggagc gctgcagttg
      241 taaactcagg aagggggata cggggccatt tccaggcaat ccggaccttg ctctcacggc
      301 atgtggcaat ggcctccctg agtacacgtc ccatgtcccc gggatccggc tcgaaataca
      361 cccgcttaca gccggcctgg caggcggcct tcacggaatc gacatcggtg cagatgattg
      421 ccagctccgg gttccgagat ccccttttca gcggacggtc cagcgtggag agccgcatgg
      481 caagcctggt acgagcctcc ccactcttcc ggttgtcagg aaggtaggtc tggaggagcg
      541 cccgctctgc ctcgctgaaa aaatggcgcc tgaacctgtt gagcgctccg accggaagga
      601 acaatccccc tccatacgtg aggctgagat cgctgacact gaatgcagtc ccccctgact
      661 tcctgatctg ccttgagatc tcgtcactgg tggtggatcg ctcctccgcc cgccccggga
      721 tgaagtcggc ctctgtcgtc accctgacaa cgcccccccc cggacctttg aacgaaccgg
      781 ataggaccgg cggtttatgg gttgcaaccg agagggcaac ctcaatcggg atcggaaacc
      841 gtcctgcagg ccctgcagca cttcggatgg ctccagccct cccttcgagc gacacactcc
      901 tggtgaggta aagtaccatc ccctcacggc acccggtcgg ctgcctgatg aggagatccc
      961 tcccggcccg caggggacgc tcacggagca caaatccgga ttcggcccgt gtcacgggat
     1021 cgatcccgac aagcccgtca cctgcggaga gggcgcgtga cccagccgcc gcaaccctgg
     1081 tctccgatcc ccggacagat cgtaccgttc cgataaaaaa tcccctgttg tcaggccggt
     1141 cacgccccat aagcgatggc ccccggtccc cgcagagata gccggaggtg aatccgcgcg
     1201 agaatgcaat ggcaagatca tccatgtccg actgctcagg gacaaaggtc ccggctgcca
     1261 ccgcattgag ggctttgcgg tagatggaga cgacgattgc cacgtagtcg ggggaacgca
     1321 tccttccctc gatcttcagt gctgtaatcg gccgttccag gattccggca aggtgcggat
     1381 acaggcagag atccttggtg gagagcagac agggctcccc gagcgggacc ctgcggggcc
     1441 tggtaattct tccgaatgaa tccggggttc ctgctaccag tgaatagtcc ctcctgcagg
     1501 gctgggcaca tgccccccgg ttcccgctcc ttcctcctat aaccgaggag agaaggcact
     1561 ggccggaata ggcataacag agtgccccat ggatgaagat ctcgataccc ggccgcttat
     1621 cgggagggac ctggaagatc cggtcgagtt cggcgagcgg gacctcccgg gcgaggacca
     1681 cccgttcgaa tccctcccga tgggcatgga gcaccccttc ccggctggtg atcgtgcact
     1741 gggtggaggc atggcgcggg aggtcgggaa cgatatcgcg tgcgatcagg gcgatgccgg
     1801 gatcctggat gagcacggca tctgccccca tcgcatacag gtcaccgaga taccgggcta
     1861 cctcgggaag ttcccggtca tggaccaggg tattgactgt gacatagacc cgggtaccgc
     1921 gcatatgcgc atacgcgagt gcctgcccga gttcctcatc attgaaattc ggactgtatt
     1981 ttcgtgcccc gaaccttttt ccgccgagat agacggcatc ggcaccggca ttgaccgcgg
     2041 ccacaagcgc gtccattgac cctgccggtg caagaagttc gggaatcccc ccctggtttt
     2101 gagacatgga acaggtatac tacgtatccc ccgcaccggt gataaaacca acaatggggg
     2161 tgatgatgag caggggactt aaatatccca ctggtctatc cactcttatg aagtgtgtcg
     2221 cctcagtctc tgacccgcgg tgcatcccgg aagcggaaga ggccggggca gatctggtcg
     2281 agctccggct cgatctcttc cccccaaccc tatcgcagga gcagaagaac tgcgtaaaga
     2341 ggtgcaccct tccgctgatc atcacggtac ggagccggca ggagggaggg tatttccgcg
     2401 gttcaccaga tgactggtgg aggctcgttc taccctggct ctctgatgcg acactggttg
     2461 acgtcgaaca ggatttctcg atgcttaccc ccctcatcaa cgatgccggg gctgaggtga
     2521 tagcctcctt tcattcgggg cggatgctga tcgggggaga actgggagcg atagcaacac
     2581 ggctccgggc atacgggacc ccgaaggtcg tggtccggcc ggagagcccg atcgatgtcc
     2641 tgacgctctg ctggttcacc caccgcacgg agaaaccggt catcaccagt gtcatcgggt
     2701 cgcagtaccg ttatgcgaga gtgatgcttc ccctgttcgg gtcagagatg gcattcgggt
     2761 atgccggtca gccaacggct gagggacagt tccatatccg ggagatgcga cagatggtcg
     2821 agaccctgat tgagtgaggt atctgctaaa aaagggatca caggcctgtt ttacaggccg
     2881 tttttccgga tatcagcccg gtacttctcc tgttcgagcc aggcatcgaa aaggaccagg
     2941 gcaagcattg cctcggcaac cggcatgagc cgggggacga tgcagggatc atgcctcccc
     3001 gtaagagcga tcgtctgtcc atctcctgaa acgctccggg ttttctgggg gagtgcgatc
     3061 gaggccgtgg gtttgaccgc gattcgtacc atgatctcgt tcccgttgct gatcccccca
     3121 aggatgcccc cggaatggtt ggagagaaac cctgtcgcgt ccatttcatc gttgtgctcg
     3181 ctccccttta ggcgtgcaga ggcaaacccg tccccgatct ccaccccttt caccgcactg
     3241 atccccatca ttgcaccagc gatctgcgca tcgagttttc caaagacagg gtcgccaagc
     3301 ccgggaggga caccggtcgc tgagatccgg atgatacccc ccaccgagtc ccccgattgc
     3361 tttgcgttcc ggatctcctc ctccattgca ccgggatccg acactccgtg gatttcctcc
     3421 accttcgccg agatctcgat cccggcccgg gcaaggaact ttgctgcgac cgcaccggcc
     3481 gcgacccggc cgaccgtctc ccggcctgat gaccttcccc cgccccggtg atcgcggatc
     3541 ccgtatttcg cctggtaggt gtaatcggca tggctcggcc ggtagacctc acggagggta
     3601 tcgtagtcag aggagcgggc atcagcattc cgtacgagca gggccaccgg catccccgtg
     3661 gtcttccctt caaagacacc ggacaggatc tccacccggt ccggttcagc ccggggcgag
     3721 gtgagcaggc tcgtccccgg cctcctccgg tcgagcagcg gctggatatc tgacacagag
     3781 agcgggagtc ccgccgggca cccgtcgatc acgaccccga tcacgggtcc atggctctcc
     3841 ccaaacgtcg tgatgcgaaa ggaccggccg aacgtgttca ttccatcgcg cccctgacaa
     3901 gctcgagaga gggtgcgatc ccggtaaagt gccggaactg ggcagcagcc tggcgtacaa
     3961 acatctcagt cccgggaatg gtctggcacc ccgagttccg ggctatcctg aggagagggg
     4021 tctcaggcgg agtgtagacc aggtcaaaga ccgtcatcga agatcggagc tggtgtttct
     4081 ggagggggct ttttagttcc ggttccatgc cgatggaggt tgcattgacg acaacggcgg
     4141 gattgagccg gtcgaacgca gaggatggcc cgtatgcaca gtcaaaccgc tctgccagcg
     4201 atgctgcttt ctcaacggtc cggttcagga tcgtcacctc catatcgagc gactggaggg
     4261 catacactgc ggcggcggca gcaccacccg cacccaggat gaccgccttc tctccgcggt
     4321 ggtcggagag gggttcccgg atcccgatcc agtcggtgtt gtatccgaaa cacctgctgc
     4381 cgcaccagac taccgtgttg accgctccga tcgcgtccgc gtgaacctcc acatcggtca
     4441 gggtgggtat gatctcctgc ttgaacggga tcgtcaccga taatccgcgg atgtctatct
     4501 cttcagcgag ccggagaatc cgcaagagat ccgggtcctg gaaccggagg tagtgggagg
     4561 tgatcccgta ctgcctgaag agccggttga acagggccgg gctcctgctc tgaattgcag
     4621 ggcaccccac gatgccataa aggcgtgtca ggggatccca caggtcaaca tcggtgagta
     4681 tcctggcttc agccacttct gattcaggaa tcccggatcc ggcaaggaac tggacaaggt
     4741 tcttcctggc ctgcaacgga accgtcccat catgaaggaa cttcctgatg gcaaggacga
     4801 cctcattcgg acttttcagg gaggtatcaa tacggaagtc tgccgccgca gcatagtgcg
     4861 gcctgcggag ttccagcaat tcatggatct cctcgcggag cggcagggtg gtaagggggg
     4921 gcctcgtgga agcagcgatc ctcttctcga tgaccacttc gtccgcttca agtaggacca
     4981 tagtgcttcc cttccgcaat gcggcgacat ttgccggatc gaggatggca cctccacccg
     5041 tcccgatcac cgcttcgcca ggtgggaggg cacggatcac ctcgcgctcc cgtgcccgga
     5101 acccctcttc accttcacgg cggaagatat cgtggatggt catccctgcc gatgcctcga
     5161 tcagggcatc cgtgtcaagg aacggcactg aagtcaggcg ggagaggagc cgtcccgtct
     5221 ctgtcttccc cgttcccctg aatccgatca gaacaactct catacaagcc catctctctg
     5281 taatatatcc cagaactccg gaaaagactt gctgacacag ccggcatctg ttatcgtgat
     5341 acccccaacg gcaagcccga gcaccgcagc gctcattgcc gtccgatgat cgcccgccgg
     5401 atcgatcgtt cctccgtgaa ggggtgctgg ccgaattgtg atcgacccgt cctttccggc
     5461 cgtgacatcc ccgccaagct gctggagggt ggagaggatg gcaaagatcc ggttgctctc
     5521 cttgaactgc aggtgggaga tcccggagaa ccacgaagga gacctggcac aggcggcgac
     5581 catgcagagg gtctgtactg tatcggggga agaggagagg tctctttcga tcccttcaag
     5641 gacaccgtca ctctcaaccg tgatgcagtc atcggagcag gatacccggc accccatctc
     5701 ggcaagggca tcgaggaact gccggtcccc ctggactgat ccgggagaga gagaggagac
     5761 ccgggctctc cctccgcaga ccgcggcaat ggcaaaaaag tacgatgcgg aggaataatc
     5821 gccctccacc tggtagtccc ttccgcaata ccccttcccg ggatgtacca cgaaatggtc
     5881 ctgcccctcc cgcaccaccg gcaccccgaa caccttcatg acatcgacag tcacatcgag
     5941 atatgaacgg gataccgccc ccggcaggag ctggagttca agcggggatg ctgcataggg
     6001 ggctgagagg aggagggagg aggcaaactg gctgctcacc gtgctgtcaa ccttcacaga
     6061 acctccgtga agggtcccgt caaccatcag gggaggaaat cctgcgttct cgagatactg
     6121 gatcctgcct cccagggaat tcagggcgtt gaccagtccc cccaccggcc gctctttcat
     6181 ccgcgggctt ccggtgatga tcacaggtct ttgacagagg agggcaacgg aggtcaaaat
     6241 gcggagactg gtcccggaat cccccatgtc aagaacggaa tggtcgccgc actgcagaac
     6301 accgcctgtc ccggtaaccc tgatctgttc cccctcccat tgcagggaga ctcccatctg
     6361 ctgcagggca gaggcggtaa tacgggtatc atcggatctg agcactcggg tgagcgtgct
     6421 tgtacccctc gcaagtcctg cggtaaccag ggcacgatgc gtgtagctct tggaagcggg
     6481 agctttgagg gtgaggtcga caggccctga acgttccagc gtgacgctca ttccggtacc
     6541 ttcacctcct ggtaacaccc caggtcgcgg aaccgggact tctcccggag ggcgacaaga
     6601 gcatcgtggc agccaggcaa gtagacaaga tcgatgaaga acaggtactc ccccatcctc
     6661 cgcttggatg gccgggactc gatcctggtg agattgatcc cccgttccgc aaagaccccg
     6721 aggatctggt acaggaggcc gggctggtct ttttccgggt cgacgaggat gctgcacttg
     6781 gtgctgccct tctcctttcc cttcgtcctt gcgatccgga tgaacctggt ggtattgccc
     6841 gggttgttct caacattccc cctgaggaga gggatcttgt agagatcggc cgtcatcctg
     6901 gagacgatag cagcagaacc gggagatcca gcaagttcac gggcacttgc cgcgttgctg
     6961 ctggtgtgga tgaccgggat cttcagctca ttcgtaatcc tgctgcactg ctcatgggtc
     7021 tggggatggg cgtagatcac ggtgacagag tcaagagggg tgaatccggc aagatggtga
     7081 tggacaggca tgtagagttc ggcggtgata tagacttggt gctggagcag cccgtcaagc
     7141 gtggcaccaa ccccacccgc ctcgctgttc tcaatcggca ccagtccgtc gccaagcccc
     7201 tgctcaacca tggaaaagat ctcgtggatg gtcggggcaa ggaggatctc gcccccgtag
     7261 acccgtactg cgagttcatg ggagaaggtt ccctccggtc ccagcacgat gagcgtcatc
     7321 gctgcaccat cgctgtgatc aggtagtcgg tctcttcagc ggcactctgg gtgatccctg
     7381 caaagaactc tgcatcgtgt gtgaagattt cacggaaggg atccggttcc cccgattcga
     7441 tcgcttcacg aatatgcgca aacgaggcct cgcaggccgc aagaacggga ggaaggtacg
     7501 gattttccgt gagcatatcc gcatacagat ccgggtcctg tgagagcagc cgcccgacaa
     7561 gccccatctc gatctgatag accgggctcc ggtagggacc ggttgcttcc gggctcacgc
     7621 caagacgccg catcgtctcg gcaaccgtga gcgtgatact gtgggtaagg ccctggacta
     7681 ctgccatcat ccggtcatgc tcttccgggg atgagagggt gatccgtgcc ccctccctct
     7741 caaagacaga gaggatggag gtgagcgcgg catcatcgat ccgtgcaggc gtggcgatga
     7801 tggtctggcc gttgagagag gggaccgtcg gaccgaacat cgggtggagg ccgacgactg
     7861 atgcccggga tgccagcatc gctgccaccg ggctggtctt cagcgaggtg aggtcacaga
     7921 ggatctgctc ctttgaaagg aacggggtaa tatcctgaat gacatgaacg gtgtcacgga
     7981 tcggcacaga taccatgagg acatctgagg agacggcaag gtcaaggcac aatgagaggc
     8041 acccccgccc gcagacctgc acctcgtgcc cggcccgcga aaaaaccccg gcaaagagcg
     8101 agcccatctt ccccgtccct ccaatgattc cgaccttcat ggcatcactt ctcaatgata
     8161 cgctccctga ccgcaatccc gaagtgcctg ccggcctgga gcaacctccc gagaacacgg
     8221 tccccggggg caagtgcaac caccgagact ggtatcccgt cctcaccgac gaggcggacc
     8281 gtctctgcgt tctggaggac aaggcttgcc ttcacctcgc ctgcttcggc ctcgaccagc
     8341 aggagcggcc ggtgctcgat cttgacccgg cctaccccgg catccctgat cgatccctca
     8401 tgggtaacgg caagcacgtg gtcccctgct ttcaggtcgg agaggtaggc agtctttccc
     8461 cctgaaacaa gggtataggc atgcactgca ccggcattga cccggaacgg ccggggattg
     8521 acatacggat tctccagtgt ctcggcctgc accagcaaca aggcagacga agtgttcccg
     8581 acaaggattc cctcaccgtc agaaagcatg gagacggtat cgacacagac ccggtcgccc
     8641 atcccgatgg gccggatggc ggtgatcgtg aacgggacaa ggtcgaccgc ccctgcgccg
     8701 gctttgacca tggtcccgat cgccctgatc agggcaggat cagcatcccg cacgagtacc
     8761 ccgcatactc ctttctcgag gaccgtcagg gctacctctg cctcctccgt gctcctgact
     8821 gctgcgataa tccgatccga gagggcaacc aggttctcca gggggatcac ccgccagtca
     8881 ccggtttcca cgatgacata gcccttttct gcaagcgcct cagcctctgc gagagatgtt
     8941 cccccctcga tcctgacctc gaagacatcg gttccccaga ccagatcccc gtcatcagag
     9001 acgacggaga ccctcccgag cgccttcaca ggtggcgctg atggggcgat gaccacatct
     9061 gccccgctct cgagcgcggt cgtgacaagt tccttcctga agggacggat atccacccag
     9121 aattctttca tcggcacccc ttcagtgcct cgtcggggtc aacctcgtcg tgcactacct
     9181 tgcagagcgc ctcgacaaat gaccggggat tgtcccgctg gaacgcgttc cttccgacac
     9241 agacccctgc cccgccggcc ttcaccgcat cgcggatggc aacgagcgtc tcgcggtcac
     9301 cggccttctc ccccccggca atgaacaccg gtaccgagca tgcggcgacg atctttgcaa
     9361 aggaggcggg atccccggtg taattggtct tgatcaggtc ggctcccagc tcctcggcaa
     9421 cacggacaca gtgcccgaca gtcgacggag cgtacgggtt gatccccttc cccctcgggt
     9481 agatcatcac gaggaggggc atcccccagc ggttgcactc cttgaccacg atggccgcct
     9541 cttcgagcat cttggactcg ttcggggctc cgagattgat atggatcgag acggcatctg
     9601 ccccgagtgc aatcgcctcc tcaaccgtac agacgagtac cttgtcgttc gggtccgggt
     9661 tgagcgaggt gctccccgag aggtggacga tcaggccgat atcccggcct ctcctccggt
     9721 gccccctctt cacgatcccc ttgtggagga cgatggcgtt tgccccgcca tcactcacct
     9781 ctgagatggt ctttgtcatg tccgtcagcc cttcgatctg gccgagcgta aacccgtgat
     9841 ccatcggtac gatcacggag cggccggtgt tccggttcat gatccgttca atccggatct
     9901 cctttcctat catcgttcat cccttcagaa attcccacgc atcttctgcg gtcttcccct
     9961 catgtacaat catcccggcg gccctgacga agcgggcagg ggtccggtgc tggaaggcgt
    10021 tcctcccgat cgagatgccg gcagccccgc cctgcatcgc gccttcgatc atctccatgg
    10081 tcgaccggtc atcggtcttt gagcctcccg ccaccacgat cgggaccggg cagccccggg
    10141 tcacggtccg gaagctgtca gggtcgccgg tgtagaccgt cttgaccatg tcggcgccga
    10201 gctctgccgc gacccgggct gccagggcga catggtcgac ctggtgctca tccttgatct
    10261 tcctccctct cgggtacatc atggcaagga ggggcatccc ccactccatg cattcgatcg
    10321 caacactccc gagatcagcc agcatcctgg cttccgagtc agccccgatg ttgatatgga
    10381 ccgatacggc atcggcgccc atcttgagtg cattcgtgac ggaattgacg agcaccttct
    10441 cgttcgggtc agggcctatc gcagtgcttg ccgacaggtg gagaatgagc ccgatgtccc
    10501 tcccgctctt ccggtggcca tggagggcaa ggccgacatg gccgagcacg gcattggcgc
    10561 ctccttcagc aacctggtcc acggcttcac cgagatcgat cagtcctgca accggcccgt
    10621 tcgtgacccc gtgatccatg ggtacgatga tggtcttccg ggtgttccgg tcgatgattc
    10681 gttccagacg gatttctttt cctctcatac aaaactccct cccatgcagc acattccggg
    10741 aatgggccca tggaatcagg caaaatactc aaaataacca aaacaggcgg gatagccaga
    10801 aacagcccgg gctcctctct catcattcgg gagaaccggt ctgcggatgc atggtgcgtg
    10861 gatgctgcta ggagagataa agaccctcac gagtgaaaaa acactcacga gcggaagtac
    10921 actcaccctg cattgtgcat gttaataggt gtcatggact atataaaact ggtgattggt
    10981 agcggctttc tccgcagttt ccagaaaaaa ttcctgttcc tggtatgagg ggaacatcga
    11041 atcgaggcag tttgtgctaa aaaaatgagg tattgaatcg cagcctttca agtgtcgtca
    11101 gggtagagaa aggggtgtat agaccgctcc ctgaagaata tccggttaaa aaataatcca
    11161 cccaggagac agaagagcta aaaaaaggtt atttctggac tttctttatc gagatgacat
    11221 ccccaagggt cgggactacc ttcccctgct tcttcttctc ttcagaaatt tcaggggtat
    11281 cgatcaggcg tatcgagagg gcacgctcga gcctgatccg gacatcgtcc tcggggatca
    11341 ggtcaccctt ctcgatcttc ttgatcagga gttccttctc cttgagctcc atggcgaggt
    11401 ccttctggga aagcccccgc tccatcctgg ccttcctgat ccgctcaccg taatcgtcca
    11461 caatctcccc ttcaatcagg tcgaggacat cccgggtctt tctgaccggt gtcgccacgg
    11521 tacgggcggc aggcccgccc ttgcgtggct cgggcttctt ctgcttctgc acctcggtcc
    11581 cgtattttgc acactggctg cagacatcga gctccgcccc ttctatccgg acggtcttgg
    11641 gaggtccatg gatggcggcg ccacacatct cgcagagtat tttagctgac tgcaccactg
    11701 taatcccctc tgatggctcg ttttattcca tttgacattg gatgctgatg atacatagat
    11761 tttttaccag atatattgat ccaaacggta ccttgaaatc cagggtacag gtgcttctgg
    11821 tcatcaacag aattgggaaa cagatctcct gacaaaaggg gatatttccc tccgggatat
    11881 ggtttgatct cctgggaata ttccttgaag gagacaaaca gtccggattg cctggataat
    11941 cgcaaacatc tttatagcca ctttccccta taggtacgtg agggaacgat ggcagaaagc
    12001 gcccggcagc cacaggagcc gcagagtcct gacgagaaga gccggttcct gcttgaccgc
    12061 gtcaatacgc tggagtccag gaacctggaa ctccgcgaac agcttcgcca gatagagacg
    12121 gacaagcggt acatcgagac ccagaagata cggtacgaac gggaggtgag gaagctcaag
    12181 agcgagatcg agcagctccg gagccctccc ctcgtcatcg gcaccgtgac cgatctggtc
    12241 gacagcaacc gggtgatcgt caggagcagt gccggtcccc gcttccttgt gcggagttcg
    12301 atgtgcatcg atcccgacca gctcaaaccc ggggtccgct gcacgctcaa ccagcagtca
    12361 ctcgcagtga tcgatatcct ccccgagagc tacgatgcag cagtctacgg gatggaggtc
    12421 gtcgaaaccc ctgatgagag ctatgcggac atcggcgggc tcgaccggca gatcatggag
    12481 ataaaggagg cggtggaact cccgctcaag cggccggagc tctttgaccg gatcggtatc
    12541 gatccaccca agggggtgct cctccacggg cctcccggga cagggaagac cctgcttgcg
    12601 aaggcagtgg cgcacgagac caacgcttac ttcctgcggg tggtcggatc cgagctcgtc
    12661 cagaaatata tcggggaagg cgctcgtctc gtccgcgaac tgttcgaact tgcaaagaag
    12721 aagtccccca ccatcatctt catcgatgag attgatgctg tcggcgcatc gaggaccgag
    12781 gctacaacat ccggcgaccg cgaggtgcag cggaccctga tgcagctcct ggcagggatg
    12841 gacggattcg agcggcgggg agatgtcaag atcatcggtg cgaccaaccg gatcgatatc
    12901 cttgaccggg cactcctgcg gccgggaagg ttcgaccgga tcattgagat cccgctcccc
    12961 gatgtggaag gacggctcgc aatcctgaat gtccacacga ggaagatgac ccttgacgaa
    13021 gaggtgaatc tcagggagat tgcctttttg accgatggga agaatggggc tgacctccgg
    13081 gcgatctgca tggaggccgg gatgtttgca atccgggagg agcatgactc catcacacga
    13141 accgacttcc tcaatgcgat cgaaaagatc gggctcgatt tcgaccgaca gaagtttgca
    13201 agcagtttcg gggcaatgtt tgcataaaat aacccggatc attttttttc ttttccactg
    13261 ctgaagggaa agaagggaag atcgtccgaa aaccgcgatg aagacctgtt cagcgggatt
    13321 gccttaccgg atttcctacg ggtctgtcgc caaaacccac cataccattt ttttctgtgc
    13381 acctcccagg cagcctgcac ccttaccgat cgccaaccag ccctcactca tcattccagc
    13441 gataaaaact aaagaagaac cggacctaat cctgaatatg cgaccagact accagaatgt
    13501 tgcaggagtc ctcctctttt tggcgggatt catcgccctg atgggtatca tcaccggaga
    13561 aatattttat cctgcgggat actcgaccgc tcacagcgag atcagcgatc tcggatcgac
    13621 acgacctcct gaagcactca tattccagcc atctgccacc atcttcaata ctaccatgat
    13681 tctggcaggg atcctgatca tcaccggcgc ctactgtgcc tcccggtcag gatgggaacg
    13741 gtggtcttca ctgctcctcg gactgctcgg ggtgggtatt ctcggcgtgg gtatctttcc
    13801 cgggaacgtc gccgtcctcc acccgctctt tgccatgttc acattcgtta tcggaggtat
    13861 cgcagcagtc ctctcatcga gaggccttcc aggcccctac cggtatcttt ccgtgatcct
    13921 tggggccatc acgctcctct ccctcctcct gatgggaatc ctgatcccgg tgctgggaga
    13981 tggcggtacc gagcgctttg tcgcctatcc cctcattttc tggctgaccg gtctcggcgc
    14041 atacttcctt ggtcgtgcca gcatgcaaca acaggcacca cacccggctt aggtggtgtt
    14101 ttaagggggc cgaccattac ccttttttaa aaaataacat gattcctttg agggccacaa
    14161 gcataaagga tgaaaatgac ggaggcattt tggcatgaaa gtcacatccg caattttcat
    14221 tagtgaggca tctgacccag cggtatcatg gacgtttcat ataaaataaa gagaagcgat
    14281 gtgactctta caccgccttc ctgaggtgtg cagccctgtt caggaagaac aaatgccgct
    14341 tcaccacctc gaatccggca ttctccgcat ctttgaggtg ttccctgaac tctcttcctg
    14401 gaacgacata gaagggctcg gtgtagaaga acaggcctcc cggcttcagg agccgtgata
    14461 tctcccaaaa caaccttgtc gggtcaggaa cctcgtgaac gacaaatatc gcacaggcaa
    14521 catcgaccgt gccatcaaac gcgggaggca gtccgatgga atccggggtg cactggtgaa
    14581 tcattacccg tttcattagg ccttcccgct ccatcttctc cctgagaatg cggagcatcc
    14641 cctcctggag gtccacagcg attaccttcc cgccgtcacc gacctggttg gcgaactccc
    14701 tcgtaaaaaa cccgggaccg caaccaaagt caaggacatg gtcgccgggc ttcacaaggc
    14761 gttctgccag gatatccggc cggtacagga ggcgccggag ccgggtatcg agctgcccgg
    14821 ctttttcagc cgagaagacc tcgtggtgat ggtgggattc ttttcgcttc ttgtcggcgg
    14881 tatctgctct ttcaggtgct gtcgttctga ccactggaca ttagattggt gtatggaaaa
    14941 taaatagggt acatcaatgc tctcctgtga tatggatctg ttcttgcttc ttcgattgga
    15001 ggtacgggca tatcactttt caaacaaatc gtctcctatt actcaaggaa caaagcgcag
    15061 atcgaatcgt gcgtgccgtt tccggcaatc tggttcttca tgaagtccac gaggttcacg
    15121 gcagagggaa tattcatggt ggtgacatag tggtgaggat tgtttcagaa aattccgaaa
    15181 ctccgattga aaaaagtata ggataagaag ttaccgtact tcagagagcc tgatataggg
    15241 gagaagtgtc aggaggtcgg atttgagccg ctcaaacccg atccgttcca ggaaccgggc
    15301 agtcctctct tttgggtggg cctgctcccg gtaatattcg aggagacgat cgacgagatc
    15361 gagggcatcg cttttcgaga ggtccgctgc aatgagggtc cccagggcag gccttcttcc
    15421 gctgttcccg ccaaagacca cgctccaccc ttttgttgtc ccaatcagtc cgacatcccg
    15481 ggtatagctc tccccgcagc aacggaggca cccggagacc ccgaacttga tctttgcagg
    15541 aaactcttcc tggtgatact tcttctccag ttcgagtcca agcccggttg aatcctggac
    15601 gccatatttg cagacatcgg ttccgaggca gatctggaca tatttgacac agggggcagt
    15661 ctccttttca gccagcgggc cgagatcctt ataaagagag gcaaggtcct tttctggaat
    15721 accgatcagc atgaaacgct gcccggaggt gagtttcatc atcgggacct tgtatttcct
    15781 tcccaccgct gcaaggttct cgagcgcttc tgggttgaca acccctgcgg gtacccgggt
    15841 caccaccgcg aacgtctttc cgtccctctg gaggattgcg ccggtatgat gaggcattga
    15901 caatactctc cctcacagta gctttgtctc ttcagttggt ttaggcacct tgatgaccag
    15961 gacccggaat acctctccgc tctcattgat ccaccggtgc ggtattcgtg ccgggctctc
    16021 gatcagcgtg tccttcccta cagtggcctg ctcttcaccg atctccacgg taccggttcc
    16081 ttccaggaca tagaagatca catcaacagg agtcttgtgc ctctttaagg attctcccgg
    16141 ctggagcgtg atcatgacca ccactgcgtg ggggatctcg cagagctttc gtgcatccac
    16201 atggtgggga tttggcgagc tggtcgccgt tattgcatca atgatcttca tacaaacact
    16261 ctccttcttt gtcttactgc aggattgtca tctctcttat caggctggat cttccactct
    16321 cgttccggcc acccttcaga tcatacggat gctttcagat cacgcatact ttacaatcct
    16381 ggttgccgca tgcaatgttc tcaatcttcc atttcagggc ccattgcttg atggcccgga
    16441 tgatcccgat gatctcaagc ccgctctcgg tcagggtata ctcggtccgg accgggaacg
    16501 cggttgcgtc caccttccgg gagatgagcg cctcctcctc gagttccttc aaccgctcag
    16561 agaggacctt gggggtgata ccgggaagag catcacggag ctctgagaat ctcctggtat
    16621 atccctctcc cttgtagagc tcaaggataa caaggagggt ccagcgcttg gacaagtacc
    16681 tgacggtctg gctgaccgtg catgaatcct gcatggtatc ttggtggaaa ctcgatccta
    16741 tttatactta agtatcatta gaatagttcg tatctaaaag agattgagag gagcctgaaa
    16801 tgttctgcta ccagtgtgaa gagaccgtga aggggatcgg atgcaccgtg aaaggtgtct
    16861 gcgggaaaga ggatgagatc gcggcatatc aggatgtgct ggtcttcctc tgcaagggac
    16921 tcgcagcccg taaccttgct gcgataaaga aaggaaaggg caatccggaa acagggctgt
    16981 tcatcgccga agcgctcttt gcgaccctga ccaacgtcaa cttcgacaag gaccggttta
    17041 ccgccatgat caaaaaagcg gtcgcactcc gggatgcgct tcccagaagc agcagcaccg
    17101 aaccggatgc ctgcacctgg aaacccggga gtgagagcga tatcctgaag aaggcagaaa
    17161 cggccgggat ccttgcgaca gagaacgagg atgtacggtc gctccggtca acgctcctct
    17221 tcggcctcaa ggggatatcg gcctactata cccatgcaga agtgctcggc aggaccgatg
    17281 attccatcac cacgttcatc cagaaggcgc ttgcttccat gctccaggac ctccctgtcc
    17341 cggacatggt ctccctggtg cttgaatgtg gaggggtagg ggtaaaaacc cttgcactgc
    17401 tcgatgaagc caataccagt gcctacggaa caccagcgat cactgccgtg aaaaccacgg
    17461 taaggaaccg ccctgggatc ctgatcaccg gccatgacct gaaagatctc gagatgctcc
    17521 ttgagcagtc aaaagatgcc ggcgtggatg tctacaccca tggagagatg cttcctgccc
    17581 acgcctatcc cgcctttaag aactatcccc acctggtcgg caactacggg agctcatggt
    17641 ggcaccagaa ggaggagttc gaccagttca acgggccggt cctgtttacc acgaactgcc
    17701 tcgtcccccc gaaagagaac tacaaggggc gtcttttcac aacagggttg acgggatatc
    17761 caggagtgcc gcacattgaa gcaggtccga atggaacaaa ggacttctct cccgtcattg
    17821 cgcttgcaaa gacctgccct gcaccgaatg ccctgccggg aagcggaagg gaccttctga
    17881 ccgggtgtgc ccatgatgcc atgctttcac tggctgacaa ggtggttgga gcggtgaaaa
    17941 acgggcagat cagaaggttt gtcgtgatgg cagggtgtga cggcaggcag aaggagcggg
    18001 agtattacac ggagtttgca caggcgcttc ccaaagacac catcatcctc accgcagggt
    18061 gtgccaagta ccggtacaac agcctggatc tgggtaaggt tgacggcatc ccaagggtcc
    18121 ttgatgccgg ccagtgcaat gactgctatt cgctcgtcgt gatcgcacag gccctcgcac
    18181 aagcgtttgg cgtcgggatc aatgatcttc cggtctccta caacatcgcc tggtacgaac
    18241 agaaagcggt cctggtgctc ctcgccctgc tgcaccttgg agtgaaggat atcacccttg
    18301 gtccgaagct cccggcattc atatcacctg cggtcctgga tgtcctcgtg aaaaacttcg
    18361 ggatccggaa gaacagcagt gttgaagagg acctgccgcg gatggtcccg gcgctctgaa
    18421 gcgaaaccaa taagagtgga accactggat tccttaacgg gaaggatgag aaaggatggc
    18481 tgaattcgac cttgaaaagg cagagaagcg cgatgagaag cgagcggagc tgcgggggaa
    18541 ggtgctccgc ctgaaggatc tcgtggaata ccaggatggc acggtcgcaa gccgcatgat
    18601 cgtgaaccgg aaggtgggga gcatcaccct cttctctttc gatgaggatg aagggctctc
    18661 cgagcacaca gccccttatg acgccgtggt caccatcctt gacgggaaat gcgaggtctg
    18721 ggtgtcagga accacgtacc agatgaaaga aggggagacc atcatcttcc cggcgaatgc
    18781 accgcatgcc ctctctgcga tcacccggtt caagatgatg ctgacgatga tcaggggatg
    18841 acgatggccg gagacgagaa agacggatac tactgctcga tctgcggcgg gatccccccc
    18901 gacaagatca agacccggcg gatcctgatc gatggcaagg agaccgggat cgatcacctc
    18961 gaattcatcc ttgaggaagt gaagaagctg catcttactg atgaatcgga gatcgtggcg
    19021 gagatcatga aacgggtcag ggtcttcaac tacgtgccga ctaagaaggc agatcggtat
    19081 gcggaggcac tgctccgcga gtataggcag caggagaccc catagatgct tgatcgctgc
    19141 ccgggggcag caaccctccg gaccccgact ctcacgatca ggaagtgccc ggagtgcggc
    19201 gaggagatcg aggtcttctc aaacgatgtc gccgtttcct gcagcaggtg tgggttcacc
    19261 gtgtacaacg atgcactctc ctgcgtccag tggtgcaagt acgcaaaaga gtgtgtgggg
    19321 gatgagacct accgcaggct cacctcccac cacccaaagg aagagggatg atgcaggggc
    19381 tccttttctg cagtattcat gacaggccgg actatcctgc caggcagagc gtcccgtcat
    19441 ttcatcaaca gtttcctggt aattgaaccg ccccttgcgg aagaaccggg cggataactg
    19501 gagtaaaatt gcgggggaga tcgggatgac ccggattcgg aataatccaa aaaatagacg
    19561 agggaagaag ctatgaaccg gaagatcatc gatatcgatc agaaaaaatg caccggctgt
    19621 ggccagtgca tcccggactg tccggaaggg gcactccagc tgatcgatgg caaagcacgg
    19681 ctggtaagcg acctcttctg cgacgggctt ggtgcctgca tggggacctg cccggaaggg
    19741 gcgatcacgg tcatcgaacg ggaggcaggg gagtacgatg agaaggccgt gatggcaaac
    19801 gtggtccccc agggacccgc ggtgataaaa gctcatcttg aacacctcgt tgggcatggc
    19861 gagaagaagc tgtatgacct ggcgatcgaa tacctcaggg agcaccagat tcccgtcccc
    19921 tctcaccggg ctcccgggtc ccctcctgac gctggtgtct gcggccatcc ctcctttgca
    19981 ggatgcccgg gatcagcagc acggaccatc ccccggagac ctcgtgaagg ctaccggcat
    20041 gcgcccggag agacaggatc cgaactgaga cagtggccgg tgcaactgaa actcctcaat
    20101 cccgaagcgc attactttga caatgccgac ctgctcatcg ctgcagactg cgtaccgttt
    20161 gcctatgcag gcttccacca ggatttcctg aaggacggga tcgtgatcat cttctgcccg
    20221 aagctcgata cagacatcga agattacatc acgaaactga aggagatctt ctctctccac
    20281 cagatcaggt cagtcaccat cctccacatg gaagtcccct gctgcagcgg ggtggcgtat
    20341 atcgtcgacc aggcactcaa agggtcggga aaaactatcc cggtcaggga gtacacggtc
    20401 atgatcgatg gcggagtcgt ggagtcgtag ccataccggg aaaaaatccg tccatccgga
    20461 cggagcagca cgcactataa aaaaataaca tgattccttt gagggccaca agcataaaag
    20521 atgaaaatgc cgtaggcatt ttcatataaa actgtaaatc agaggtccct gcaccgggaa
    20581 cctcccttga acaccgtccg gtcgaccagc gaggatacct ctggcggcca tatgtacgat
    20641 cggtgccagc ggtcatctcc tgccctgcag gaaccccggc gccagcccac tcacctcacg
    20701 cactactgat gccaccggcc tggctgctca tgtgcaggct tgatgagaga tgaggcgcaa
    20761 ccagaacaaa gagactcccg gccggtgaga tctcccagga taccaccaga agaaagggga
    20821 tactagtccg tgaatgcaat cgaagtggag cacctcacca aaaggtttgg cactgtcact
    20881 gcagttgacg atatcagcct ctccgtgcag aatggcggga tcttcggatt cctcggaccg
    20941 aatggtgcag ggaagaccac caccatccgc ctgatcaccg gagtcctgac gcccgatgca
    21001 gggagtgccc ggatcagggg tatcgacatc caccgccacc cgatcgatgc caagatgcag
    21061 atgggggtca tcccggagag cagcaccgtc tatggtgatc tctccgctga acagaacctg
    21121 cacctgaccg ggaagatgta cgggatgccc cggccgatcc gggaagagcg ggttgacgaa
    21181 ctcctcacaa agatgggtct cctggcacag cggaaatacc cggtccggac cttttcgaag
    21241 gggatgaaac agcgggtcag tatcgcctgc gcaatcatgc accggcccgc tgtcctgttc
    21301 cttgatgagc cgacaagcgg gctcgatgtc cagagccgga gactggtggt cgagacgata
    21361 caccagatga acgaggaggg cagtaccgta ttcctcacca cccataacat cgaggaggca
    21421 aacaccctct gcaggaccgt ctgcatcatc aaccggggaa ggattgttgc acaggatagc
    21481 ccggagcgtc tcaggaggat gtttgaggcc acgcagtcgg tggaggtctc gttctcccgg
    21541 ggcgtccgga acgatttctt ccaggcagag gggatcatcc gggctgagcc atgcggcgac
    21601 aagtggcgga tctacacgga caaccctgac cgtgtggtaa aataccttgt cagccgggcc
    21661 gaccgggagt cactcgggat cacgtcgctt gccatcaccg gccccagcct ggaagagact
    21721 tttgtccagc tgacggggtg cacatgaaaa cacctgtggt gttcctccgg ggcattctta
    21781 ccatctgtga gaagaacatt ttcctgtact acacaaaacc cccggtgctg atgttcggca
    21841 tcctcttccc gatcttcatg ttcctcgcct tcttcgttgg ccggaacctc gacctcgttc
    21901 tgttcttccc ggggtttctc gcgatgatgc tctttttcac cgcatcttca gttggtcccc
    21961 tcatcacccc atgggagaag cgggagaaga cctacgagcg gctcctctca tacccggtca
    22021 tcccagagag cattatcatt ggtgatatcc tggcgggtgc gatcttcggc ctgggcatct
    22081 ccctgatcat ctggatagta agtaccctgt tcattccaat gcatatcatc aatcccgccc
    22141 tgttcctgtt caccttcgtg ctggggacga tcgcatgctc ctcccttggc gccctgctgg
    22201 cctcccctgc ctccaacagt ccgccgaacg tgatgatctt ttcaaacctg atacgcttcc
    22261 cactcatctt catatcaggg atattcatac cgctctccca gatgcagggg ctctcccttg
    22321 cccttgcctc ctgttcaccg ctcacgtacg tggtggatct cttcaatgca gcgatatccg
    22381 ggggatcgtt cttctctccc ctccttgacg ggggcgtgct cattgctgcc accgttctgt
    22441 tcatcctgct cgcccggttc atccaggtac gtaacctggt caagggactg tgaaaagggt
    22501 tcggacccgg gctgctgcaa gaacagtcat atagtcgagg aaactggtat atcgcaacaa
    22561 ggaaccgatc actgattgtg aacagcggga aagggatacc ccgacctgct accggatgtt
    22621 cagcggatca taagaacgga cacattccga ccggaaacgg tacactgatt ggattgtgga
    22681 cctcaggaaa gccggggtca gcgggaatgt gaggatacaa gggggcatag tttagtattc
    22741 caggcgaaat ttcccacagg atccggaaaa gaacacgtct ttttacagca aaggtgggta
    22801 gtgtagcttc gttgcccaaa cagtgtaacc gggctgctgc tcaggcagaa gttcgcctgg
    22861 ccgcaaagga atattcaatt agccgcgggg gataacaccc tacaaggaaa ggtgatgacg
    22921 ttatgactga tgatgccagg aacccggcaa aggccgggat gaaccaggga ccaaccggca
    22981 gaggaaggtc catccgggac tggtggccga accaggtgaa cctgaagatg ctccaccaac
    23041 actcccccct gtccaatccg atgggcaggg agttcaacta cgctgaggag ttcaagacac
    23101 tcgacctcgc agccgtgaaa tcggacctgc gggcgctgat gaccgaatcg caggactggt
    23161 ggccggcgga tttcggccac tacggccccc tgttcatccg catggcatgg cacagcgccg
    23221 gcacgtaccg cgttggtgat ggccgtggcg gcgccggttc cgcccagcag cgtttcccac
    23281 cgctcaatag ctggcccgat aacgcgaatc tcgataaagc acgccggctg ctctggccga
    23341 tcaagcagaa gtatggccgc aaaatctcgt gggccgacct cctgatcctt gccggcaatg
    23401 tcgcccttga atcgatggga ttcaggacct tcggcttcgc cggcggccgt gaggatgcct
    23461 gggaaccgga cgagagtgtc tattggggtg cagagaacga gtggctgggt gacaaacggt
    23521 attccggcga ccggaagctc gaaaatccgc ttgctgccgt gcagatgggc ctgatttacg
    23581 tcaatcctga gggtccgaat ggcaacccgg atccggtcgc ggcggccagg gatatcaggg
    23641 aggtcttcgc ccggatggca atgaacgacg aagagacggt tgcgctgatc gccggcggtc
    23701 atgcgtttgg caaaacccat ggtgccggcc ccgcatcaca cgtcgggccg gagcccgacg
    23761 cggcccccat cgaggagcag ggcctcgggt ggaagagcag ttacggcacc ggcaaaggga
    23821 atgatgcgat caccggtggc ccggaagtga tctggaccca gacgccgatc aagtggagca
    23881 acaacttctt ccggaacctg ttcacgtttg agtgggaact ggaaaagagt cctgccggcg
    23941 cataccagtg gaagccgaag ggcggcgccg gtgccgggag tgtgccggat ccccatgatc
    24001 cctcgaagcg tcgcgctccg ggcatgctga ccaccgacct tgccctccgg ttcgacccag
    24061 tctacgagaa gatatcccgg cgcttctacg agcacccgga gcagatggca gatgcattcg
    24121 cccgggcgtg gttcaagctg acccaccgcg acatgggtcc acgttcccgc tatctcggcc
    24181 cggaagttcc cgcagaagaa ctcatctggc aggaccctat ccctgccgtc gatcaccccc
    24241 tgatcgatgc gaaggacatc gcatccctca aggccatgat ccttgccacc ggcctttcgg
    24301 tctcagcgct ggtctcgacc gcctgggcat cggcgtccac cttccggggc tccgacaagc
    24361 gcggcggtgc caacggtgcc cgtatccgcc tcgcaccgca gaaggactgg gaggtcaacc
    24421 agcctgcccg gctggcgaag gtgctcggga cccttgaggg catacaacgg gagttcaaca
    24481 gtaaagccac gggcggtaag aaggtctcat tggctgacct gatcgtgctg gccggttgtg
    24541 ccggtgtcga gcaggccgca aaaaatgctg gtcattcggt gacggtcccc ttcaccccgg
    24601 gacgcatgga cgcctcccgg gagcagaccg atgtggactc cttcgctgtg ctcgagccga
    24661 aggcggatgg tttccgcaac tacctgaagg gcaagtattc tgtatcggca gaggagttgc
    24721 tggtcgacaa ggca
//
LOCUS       DAOOCO010000009        20476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2281_length_20476_cov_5.341119, whole genome shotgun sequence.
ACCESSION   DAOOCO010000009 DAOOCO010000000
VERSION     DAOOCO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 20476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20476
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_20476_cov_5.341119"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1569
                     /locus_tag="PK154_01125"
     CDS             43..1569
                     /locus_tag="PK154_01125"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HPW09697.1"
                     /translation="MKLSVKLIDIGTKEVLLNSGDARNIGVLAGDRVQILNQKTGVSI
                     AAFVDTTTSLIPEGVVGIYRITNEKLGVEEGSSVEVRLAERPVSLQFIRKKMDGIRLT
                     KEETYEIVKDVVNEDLTPAELTAYITACYINSMDMDEVEHLTRAMVDTGERLTFHTHP
                     IIDKHSIGGVPGNKISLIVVPVIAACGLKIPKTSSRAITGAGGTADLMEVLAPVEFSA
                     AEVQSMTLKVGGTIVWGGATNIAPADDHIIIQEYPFKIDARGQMLASVMAKKFAVGAD
                     LVVIDIPVGEHAKVPNVQEGRKLAREFIDIGERLNMRVECALTYGDSPVGRSIGPNLE
                     VNEALRILEGSDEPGSLLQKSIAIAGIALEMSGKAPRGMGSKMALDVIKKGDALKKFR
                     EIIAIQGGDPNVKAADIVPGKYEFVVNAPSDGYVIEMNNKSLISLARSAGAPHDHGAG
                     ILLHAKKGSRVEKGEPIFTIYAERSWRLQNAVEDGRRLMPVLVEGMLLDRVPHELKWE
                     "
     gene            1656..3044
                     /locus_tag="PK154_01130"
     CDS             1656..3044
                     /locus_tag="PK154_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW09698.1"
                     /translation="MQRYGVTLFIAIFMLLIMTVTSCGAVVIIQVIEKDDFIAPVQGA
                     LVYSNGTPIGKTDEEGLLQVLSPGSDSVPLRVEKSGYDTWEGSIGPKTDELLVELQKA
                     ELSLSVHLYDADTMDPLAGVNVTLAGEVAPTSVISDQNGTAVFPVQAKGSYQIRAEAE
                     QYQPVSMEVEAGFADKEVQVLLFRGDRFSLVIKDGESGETLQGARVSVEGIERGTSDS
                     RGILNLPMPRDKVYLIRVVMDGYQEYNGHQIVGSDTAFLTIPLVKAPFTVFVSVSNED
                     AEPVDGALVLVNSTPMGRTGRNGRLVLMNLTAGLYQVDIQHPDYVPTQHMVTVATQGE
                     DITTELTYRQENRTIKTVEGPGNPVAGVRISLNSQEEGSTGESGTLAVTLRMNQNYTI
                     TAEKDGYHPVVIERMFSSADGASVITIPMEQEYNWLFLGAAGIGIIVIILGILLLWRR
                     THTSSHGKGGGL"
     gene            complement(3441..3512)
                     /locus_tag="PK154_01135"
     tRNA            complement(3441..3512)
                     /locus_tag="PK154_01135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3477..3479),aa:Arg,seq:tcg)
     gene            3570..4007
                     /gene="dcd"
                     /locus_tag="PK154_01140"
     CDS             3570..4007
                     /gene="dcd"
                     /locus_tag="PK154_01140"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPW09699.1"
                     /translation="MILSSEEIQRRLGDQSLNGDLVITPYGSECQQPASYDLRVAEDT
                     ELKPGICTLTHTLERVELPADIAATIRTRSSYGRRGLLVTAGFVDPGFRGQLTLGLVN
                     MGQEVIHLKRHDRVVQLILHQVIGAGQTYEGRYQDSSGIVRAR"
     gene            4012..4962
                     /locus_tag="PK154_01145"
     CDS             4012..4962
                     /locus_tag="PK154_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPW09700.1"
                     /translation="MIRTERKYIEILRILKEHQEPMGAKRLSELMAERGFVLSDRAVQ
                     YYLRYLDEMGFTEKVGNMGRILTPYGMNETESALVGERIGFIISRLERLAFRSTFDPV
                     TQTGDVAYNLSIVPEERFDEVLNAFDAVAAARYGFFSSYRVLDNDSRVPEGSVGIITV
                     CSITMDGVFQNRGIPVHMAFGGRLSIEHGEPIRFVDLIGYRGTTIDPLQLFISAGLTS
                     VGSMIATGTGIALANVREVPAAAESQVRETVAQMRKSGFVFPVIMGPAPFNLPSDPYR
                     ISLVSYSGMNFVASVFEKKIPIRTEIGAGNIPFSRIMPPN"
     gene            complement(4984..6156)
                     /locus_tag="PK154_01150"
     CDS             complement(4984..6156)
                     /locus_tag="PK154_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09701.1"
                     /translation="MRVLIVGIGGAGCRIADAIYDDDKKSSSLRCTDGIAIDSDASSL
                     TTLRALPKENLFFSKTLEPNHDEKTPTSLPNDEIIARLQSLDHGDIDALIICLGLGGT
                     MAGLAPSLILSIRKTMIEPVFGLFTLPREAEAEEVRIQAANQIDALSGIMDGVLLFDN
                     EKWIAKVANDPDLQGIQHTEGVPLPFKRSQSIPLEAITSGYEGVNHVIGRWISLILRA
                     GEVSERPGSEVGEVALDAGEIVNTLRGCGLAAIGFARDPISAVSPDLIRKLRPAGHSV
                     DESHEKAARIIALGKRAVLEEMSVSCNIADAEKALILIAGPSHELNMRGYMAFRHWID
                     VNISGFEVRSGDYPIKNSRFVGVAVILSGFSHIARVDSLRAIRDEKILSQSHGIAG"
     gene            6434..7423
                     /locus_tag="PK154_01155"
     CDS             6434..7423
                     /locus_tag="PK154_01155"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HPW09702.1"
                     /translation="MTTYGIEFVPGNVNVKQVVNFCKLAESKDIDFAWITNHYNNRHC
                     YPTLAAIAQATTTLKMGPGIMNSFTDTPAAMASFFCTLNEISDGRAVLGIGPGDLSTL
                     PKLAIAAEKPVARLEEAVTQIRRLCAGEEVKKSGMQFFDYDGAKLTGVTLPGKKGVPI
                     YIGAQGPKVLDLAGRVGDGALINASNPKDFAIAIPIIKAACDKVGKKGFDVGAYTAMS
                     IDMNEKKARNAAKIVAAFIAAGSPPDLIARHGLDAAKVATIKGALAKFDFKTAGENVD
                     DKTIDAFTIAGTPDTVKQKCEDLAKAGVTQIIFGSPLGPDMTTSIRLLGKYVV"
     gene            7502..8449
                     /locus_tag="PK154_01160"
     CDS             7502..8449
                     /locus_tag="PK154_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPW09703.1"
                     /translation="MFTVTEIRTGTQPLQVREELLTGHRCKISPGRLNRGIDIPYHPQ
                     DIQENCPFCRERIFSVTPVFPDGKRICRGESITVPNLFPFGEWHTVTVITSRHVVDTF
                     SPGEIADALSGQSESLMRAEGYKSINWNYLPSSGASIAHPHLQGLADRRAPVTAERYL
                     VAGQRYLMKQGRPYWEDLRSFESGSPRYLFGDELFWYAHPVPLGEREVRCLLPIATLE
                     EFTPYIETFAEDLLRVIGMYRRLGTYAFNISVFFDKKKEKNGFSSFCSIISRINPNAY
                     SMSDSAFMERLHLEPVILTLPEDLADIFRNEYCYSRVKS"
     gene            complement(8513..9352)
                     /locus_tag="PK154_01165"
     CDS             complement(8513..9352)
                     /locus_tag="PK154_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HPW09704.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADREDMQTFMATSGTKLQ
                     PEDIDRVVNNMVAWKPDFAIVVSPNGVLPGPTGARERLAAAGIPTLFITDDVTTKKEW
                     AEIKDGKFGYIIMKGDAMIGARREFLDPIEMADYNGNLVKVLALTGAFRKLQNAIDQV
                     IDQVKAGKKGADLAMPKLIVTSDKAVDGEFSNPYALAKARAAYEIASAVAMVNVKGCF
                     MTKEWEKYIPIVTSAHEMMRAAAVLCDEAREIEKAGDGIIRKPHKKDGIIVSKTKLIS
                     KPE"
     gene            9572..9976
                     /locus_tag="PK154_01170"
     CDS             9572..9976
                     /locus_tag="PK154_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HPW09705.1"
                     /translation="MKDGLLTDRQKEVLRYRKQGMTQQQIADIIRTSKANVCTIEKSA
                     MENIRRAKETLEFLYTLDATHLCTIESGMDLLDVAPYIYREAENIGIKVKYDTISLIN
                     KLREATPERFRARHVRDAIEVYINEEGDLYFG"
     gene            10568..11581
                     /locus_tag="PK154_01175"
     CDS             10568..11581
                     /locus_tag="PK154_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPW09706.1"
                     /translation="MPKINTPRKGSLAFSPRKRAKGPVPKYQSWPEYEGTPVLQGFAG
                     YKVGMTHIVMVDDHKNSPTEGKDIMVPVTVIEIPAMKVAAIRAYTKDTYGKHPLTEVW
                     EAAPDPALDRRITLPKTYDGSAAQKAILDGIKAGTVAEIFALVHTTPELVSGIPKKVP
                     DIMEVRIAGGALENRFEFALSLLGKEVTLKNVLQAGSYADITAVTTGKGTQGPVKRWG
                     IKLRKRKHSRGGKKRHVGNLGPWNPHHVRWQVPQAGQMGYQQRTEFNKRILKIGDNGK
                     EVTPDGGFLHYGVLNNPYVLVKGSVPGPVKRLVRIRPAMRLGEHVPRQPAIEFVSVQS
                     KQG"
     gene            11589..12335
                     /gene="rpl4p"
                     /locus_tag="PK154_01180"
     CDS             11589..12335
                     /gene="rpl4p"
                     /locus_tag="PK154_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPW09707.1"
                     /translation="MKAQVITIDGTRKGDIDLPPAFSEEYRPDLIRKAVIALQSTRRQ
                     PHGTYPYAGIESSAVGWGSGRGVSHVPRIKTGSRAAKVPQAKGGREAHPPVVQKVLLL
                     RMNKKEKAKAFRSALAASASEEIVRSRGHLFTGQIPVVFEDSFGELGRTSEVISALTA
                     AGLYQDIERSKSSRKVRAGRGKMRGRRYKQRKSLLIVTAEKPLRAARNLAGVDVVTLE
                     QLNTELLAPGTHPGRLTVYTESAISRLGGQ"
     gene            12337..12585
                     /locus_tag="PK154_01185"
     CDS             12337..12585
                     /locus_tag="PK154_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPW09708.1"
                     /translation="MVLRHPYVTEKAMMTLEKENKLQFLVDKDATKKQIKREIEKTFE
                     QEVRGVSTVMTMHGEKKAIISFANEKAAEEILSRLGIM"
     gene            12595..13311
                     /locus_tag="PK154_01190"
     CDS             12595..13311
                     /locus_tag="PK154_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPW09709.1"
                     /translation="MGHRITTQNRGKGGPAYRVPSHRYKAALRHASSGTTTVKGIVVD
                     IEHDPARHTPIALVKLEDGSRMYMLVTEGIGVNDEVSWGPEGEVRNGNTLPLSAIPTG
                     AYVCNIEARPNDGGKFVRASGVQAIVVDKTAERVGVQMPSGVTKWFNASCRATIGIVA
                     GGGRGEKPFVKAGNKFHKMKNSASNWPRVRGVAMNVIDHPFGGGGHQHAGRPKTVARG
                     TSPGRTVGHVAARKTGRGKK"
     gene            13321..13734
                     /locus_tag="PK154_01195"
     CDS             13321..13734
                     /locus_tag="PK154_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPW09710.1"
                     /translation="MAKKTQKRLPRRREEFTYRGFRLDDLVKMGTADLLPIMPARVRR
                     KVSRGLTRGEEHLLSRFKSGDARIRTHLRDMVIMPDMIGKEVEIYNGKEFMKVEIQPE
                     AIFHYLGEFALTRRKVTHGSAGIGATRSSKYVPLK"
     gene            13740..14201
                     /locus_tag="PK154_01200"
     CDS             13740..14201
                     /locus_tag="PK154_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPW09711.1"
                     /translation="MARTGYSTDITGDTVARGKANELPISPKHAMEIARFIRNKKTGE
                     VVSYLEDVVAEKKAIPFRRFNHKVAHKRGLSGWDAGRYPRKASKAYLRLLHSVTKNAE
                     YLGLDTERLEIVHVAANRGRGFEGIFPRAMGRASPKRRETVNIEIVVKEVQ"
     gene            14202..14918
                     /locus_tag="PK154_01205"
     CDS             14202..14918
                     /locus_tag="PK154_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPW09712.1"
                     /translation="MAVERKFVAEGARRARVEKHLVKELKRAGYGGMDLQRTPLGTQV
                     TIYAEKPGIVIGKGGKVVRQLTQDLATDFGVESPQIEVQQVPNPSFNAQIMAERLANA
                     LERGWYFRKAGSSIIRRVMESGALGCEVIIAGKLTGARARVQKFTEGYIKHSGEPVNT
                     IVEKGFAVAIKKLGVIGVQVRIIPPESKLPDHFEMIVPKQKLVRKEEIAITPVGEEIE
                     EEDLAMDLDEFDDVETPEDR"
     gene            14918..15121
                     /gene="rpmC"
                     /locus_tag="PK154_01210"
     CDS             14918..15121
                     /gene="rpmC"
                     /locus_tag="PK154_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPW09713.1"
                     /translation="MAILRASDIRQLSDVELQEQMEKLRIELIQHNGKVSAGGSTENP
                     GRIRELRRTIARMMTEQNHRRTV"
     gene            15118..15402
                     /locus_tag="PK154_01215"
     CDS             15118..15402
                     /locus_tag="PK154_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HPW09714.1"
                     /translation="MISARNILRHELIGLVVSVVDASNPLHRGISGQIIDETRNTLLI
                     RTPDGDRRIQKQYTRFCISIPDGTRVLIDGSAIVSSPEKRITQYAKIRGK"
     gene            15402..15728
                     /locus_tag="PK154_01220"
     CDS             15402..15728
                     /locus_tag="PK154_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPW09715.1"
                     /translation="MAKNIGLGVLPPEQACSDANCPFHGSLPVRGQVITGKVVSDRMM
                     GTVVVERNYLHFVRKYNRYEKRSSRIHAHNPPCLQAKVGDDVKIAECRPLSKTTTFVV
                     VEVQRL"
     gene            15725..16123
                     /locus_tag="PK154_01225"
     CDS             15725..16123
                     /locus_tag="PK154_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPW09716.1"
                     /translation="MKAKQSKTPRSLSTGTRMICADNTGAREVQIVSVFGYHGVRRRQ
                     PKMGLGDLATVTVKKGTPDMRRKLVRAVVIRQRKEIKRPNGLRITFDDNAVVVVDEKN
                     EPRGTEIKGPVAREVAERFPKIGSMATMIV"
     gene            16136..16501
                     /gene="rplX"
                     /locus_tag="PK154_01230"
     CDS             16136..16501
                     /gene="rplX"
                     /locus_tag="PK154_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPW09717.1"
                     /translation="MVRIESTQPRKQRKARYNAPAHLRGKFISAPLTDELRTQYKRRT
                     VRVVKGDTVKVLRGDAAGTEGLVDDVDTGRSLLIIQGVSIPKADGTEVPRPVDPSNVQ
                     ITKLNLKDPKRAEKMAEVR"
     gene            16501..17226
                     /locus_tag="PK154_01235"
     CDS             16501..17226
                     /locus_tag="PK154_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HPW09718.1"
                     /translation="MSDHLKRLRAPDSWHIAKKTSTFVAKTAPGPHNANAMPIAVWLR
                     DHMNFARNMKEVKQILGQNDVIINGRPCRDPRMGIGIFDIISLPKIGKHYRILRDKKG
                     RHKTIEITAESAKTRLAKVRNKTIIPGGKVQLNLRFGANVLGDNQYKPGDSVVISLQP
                     EDRFTITDHFPFAVGNMAMVIGGRHSGRIARITAIEKVPGSVPNRVLLADEKAGTTFD
                     TIDAYIYMVGRETPALAEWGIEE"
     gene            17223..17729
                     /locus_tag="PK154_01240"
     CDS             17223..17729
                     /locus_tag="PK154_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPW09719.1"
                     /translation="MNPMREVFVDKVVVHMGVGESGDKLTKAVDIMKKITGNNPVKSV
                     AKKTNPAFGIRKGAPISCKVTLRGETAGDFLDTALAIIERKVSRDQFDKSGNFSFGIE
                     EHTDFPGMSYDPQIGIYGMDINVILARKGVRIARRNIERHRLPQKQMVQPEDAIAFLK
                     SRYQAEVA"
     gene            17729..17884
                     /locus_tag="PK154_01245"
     CDS             17729..17884
                     /locus_tag="PK154_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPW09720.1"
                     /translation="MGGEKTKKFGRGAFACKICGRKQGLVRRYHIMFCRQCFREWAQK
                     MGFKKMN"
     gene            17898..18290
                     /locus_tag="PK154_01250"
     CDS             17898..18290
                     /locus_tag="PK154_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPW09721.1"
                     /translation="MGRLNTIADGMSALKNAADGGKPGVLIEPAGKLLGAMFRIMQES
                     GYISGFEYVDDGRGGQFLVQLNGRINKCGAISPRYSVPLNELEYWETQYLPGKNFGIL
                     ILSTSKGVMTHEQARKEGVGGELLGYVY"
     gene            18303..18827
                     /locus_tag="PK154_01255"
     CDS             18303..18827
                     /locus_tag="PK154_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPW09722.1"
                     /translation="MASERKVTIPEGVKVELEGTLLKVKGPKGQLERIVRFPQITTVL
                     ADNTVIFSTNSDRKSVIAMVGTFAAHTRNMCHGVNTGFEYRMKVVYSHFPIQLKLQGN
                     KLEINNFLGEKKARYATIVNGVTAKIGNDEIILSGIDKELIGNTSANIEHATKIRNRD
                     PRIFQDGIYIVERA"
     gene            18833..19243
                     /locus_tag="PK154_01260"
     CDS             18833..19243
                     /locus_tag="PK154_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HPW09723.1"
                     /translation="MADETKRLIQVRSDKSASFKRDGAGKKKQLSESWRMPRGLHSKK
                     RRQKKAKGPLPTPGYGSPIEVRGMHPSGYREIRVFTPGDLAGLNPDEDAIRIGGTVGN
                     KKRMLIQDQAVAAGLKVLNARDIRPKEQEAKSDD"
     gene            19236..19688
                     /locus_tag="PK154_01265"
     CDS             19236..19688
                     /locus_tag="PK154_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HPW09724.1"
                     /translation="MTDIANQRRLAASLLGCGINRVWFDAERIGDIQNAISREDIRGL
                     IGEGVIRARQVQGNSRGRARALMAKRSYGHCKGPGRRKGAAGARNPSKREWIQKIRAI
                     RKTLAELRESGEIAPHLYRTLYRKAAGGQFRNVAHLKAQIGIISGRMK"
     gene            19690..20217
                     /locus_tag="PK154_01270"
     CDS             19690..20217
                     /locus_tag="PK154_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPW09725.1"
                     /translation="MATGPRYFVPFRRRKEGKTDYYKRAKLVVSDRPRMVVRKTNRQI
                     IIQMVLAEMDGDRTLVSASSTELANYGYNGSTSALPAAYLTGMLFAVKALNADQQRAV
                     LDIGLHRATPGNRVFAALKGAVAAGLDVPHGEQVLPDDDRVKGAHIAAFAPERAGDLV
                     RNVDETIDAIMKELR"
     gene            20219..>20476
                     /gene="rps5p"
                     /locus_tag="PK154_01275"
     CDS             20219..>20476
                     /gene="rps5p"
                     /locus_tag="PK154_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106091.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPW09726.1"
                     /translation="MPYEKTEWVPLTGLGKMVASGEITSIDQVLESGRPIKEPEIVDA
                     FLPDLEDEVLDIEMVQRMTDSGRRVKFRAVVVVGNRNGYIGF"
BASE COUNT         5259 a         5037 c         5746 g         4434 t
ORIGIN      
        1 attcatctcc cctggatgtg gtgtagccag gatctgcaaa ccatgaagct gagtgtcaag
       61 ttgattgata tcgggacaaa ggaggtcctc ctgaatagtg gcgatgcacg gaatatcgga
      121 gtccttgcgg gcgaccgggt acagatcctc aaccagaaga ccggagtctc aatagcggcc
      181 tttgttgata ccacgacatc gcttatcccg gaaggggttg tcggaattta ccgaatcacg
      241 aatgagaagc tgggtgtgga agaggggtct tcagtagaag tcaggctggc agaacgaccg
      301 gtttccctgc agtttatccg gaaaaagatg gacggtatcc gcctgacaaa agaagagacc
      361 tacgagattg taaaggacgt cgtgaacgaa gaccttactc ctgctgaact tactgcctat
      421 atcaccgcct gctacatcaa ctccatggat atggacgagg tggaacatct cacccgtgcg
      481 atggtggata ccggggaacg actcacgttc catacccatc cgatcatcga taaacattcc
      541 attggaggtg ttcctggcaa caagatctca ctcatcgtgg tcccggtaat tgccgcctgt
      601 ggcctgaaga tccccaagac cagttcccgg gcgataacag gagcaggagg caccgctgac
      661 cttatggagg ttctagcccc tgtcgagttt tcagcagcag aagtccagag tatgaccctg
      721 aaagtcgggg ggacgattgt ctggggtggt gcaacaaaca tcgctcctgc agatgatcac
      781 atcatcatcc aggaataccc gttcaagatc gatgcccgtg gccagatgct cgccagcgta
      841 atggcaaaaa agtttgcggt gggagctgac cttgttgtga tcgatattcc ggttggagag
      901 catgcgaaag tccccaatgt tcaggaaggg aggaaacttg ccagggaatt cattgacatt
      961 ggtgaacggc tgaacatgcg tgttgaatgt gcactgacct acggggactc tcccgtggga
     1021 aggagcatag gcccgaacct tgaagtgaac gaagcactgc ggatccttga aggaagtgat
     1081 gaacctggct ccctgctcca gaagagtatt gccattgccg gtatcgccct tgaaatgtcc
     1141 ggcaaggcac cccgtggcat ggggagtaag atggcgcttg acgtcatcaa gaaaggcgat
     1201 gccctgaaaa aattcaggga gattattgcc atccagggtg gtgatccaaa cgtgaaagct
     1261 gccgatatcg ttcccgggaa atacgagttt gtcgtcaatg caccgtccga tggctatgtg
     1321 atagagatga acaacaagtc actcatcagt ctcgcacgat ctgcaggagc accccatgat
     1381 cacggtgcag ggatcctcct ccatgccaag aagggaagca gggtggagaa gggagaaccc
     1441 attttcacga tctatgctga gcggagctgg cgactccaga atgctgtcga ggacggacgc
     1501 cgccttatgc cggtccttgt tgaagggatg ctccttgacc gggtcccgca tgagttaaaa
     1561 tgggaatgag agggagattc cccattccgt acctttcttt tccttcatca ctgacgacaa
     1621 ccactattat aacagagcac ctctgatatc agactatgca gagatacgga gtaactctct
     1681 tcattgcaat cttcatgctc ctcatcatga ctgtgacatc ctgcggggca gttgtgatta
     1741 tccaggtgat agaaaaggac gattttatcg ctccagtcca gggtgctctt gtttattcca
     1801 atggtacccc gataggaaaa accgatgagg aaggattgct ccaagtcctg tctccaggca
     1861 gtgattcggt gccactgaga gtggaaaagt ctgggtatga tacctgggag gggtctatcg
     1921 gaccaaaaac ggatgagctt ctcgtagaat tgcagaaggc agagctctcc ctttcagtcc
     1981 acctgtacga tgcagacact atggatcccc tggcaggggt gaatgtgacc cttgcagggg
     2041 aggtggcccc cacctctgtc atctcagacc agaatggtac tgcggtgttt ccggttcagg
     2101 caaagggatc ctaccagatc agagcagagg cagaacagta tcagccggtc agtatggagg
     2161 ttgaagctgg gttcgctgac aaagaggtcc aggttttgct cttcaggggg gatcgtttct
     2221 cactcgtgat aaaggatgga gagtcgggtg aaacgctgca gggggccagg gtctccgttg
     2281 aaggcattga gagggggacg tcggactcca gggggatact gaacctgccg atgcccagag
     2341 acaaggtata cctgatcagg gtagtgatgg acggatacca ggaatacaac ggccatcaga
     2401 ttgtggggag cgataccgca ttcctcacga taccgctcgt caaagcccct ttcacggtct
     2461 tcgtctctgt ctctaatgaa gatgcagagc cggtggatgg cgctctggtt ctggtcaata
     2521 gtactcctat gggaaggaca ggccgtaatg gacgtctggt tctgatgaat ctcactgctg
     2581 gactctatca ggtggatatt cagcatccgg actatgttcc cacacagcat atggtcacgg
     2641 tggcaacaca gggagaggat atcaccaccg agctgacgta tcggcaggaa aatcgaacta
     2701 ttaaaacggt tgaaggtccg ggaaacccgg ttgccggtgt caggatcagc ctgaatagtc
     2761 aggaagaagg gagtactggt gaatcaggta cacttgctgt aacgcttcga atgaatcaga
     2821 attacacgat aactgcagag aaagacgggt atcatccggt tgtcattgaa cggatgttca
     2881 gttctgctga cggcgcctcg gtgattacca tcccgatgga acaggagtac aactggttat
     2941 tccttggcgc tgcagggatt ggaattattg tcatcatctt gggtatcctg ctcctttgga
     3001 gacgaaccca caccagttcg catggaaaag ggggagggct ttaactcttc ccgaggatta
     3061 cccgtcccgg tgttccctca aggtgacacc cggtgtctct gttatctttt tcttctgact
     3121 atcggttagg aagtggggaa aaacaagctt ttcgctgttc tggtgtggcg aaacaaagag
     3181 aaaccacatt attccgtagg actatctgtc cccacactcc actctgatgc acccgccgtc
     3241 ccggaagagc gccattgcag ctgttcattc agcatctttt cccccggata aaatcccggg
     3301 ctggtttcct gcctatcaca gctggcaggc agaaaaggaa tcaaaagaat cactttccct
     3361 ggagaggatc cgtgcattcc gaatgatatt ccagagtgcc aaaagaggcg cagagcaggg
     3421 aaggcctgac gattggcaag cggacccagc gggaattgaa cccgcgtccc tgggtccgaa
     3481 gcccaggagg atgtcctcta ccccatggat ccgtccatct cattggtatc ataagatatt
     3541 aagcattttc cgggctagta ttgtacgaga tgatactctc ttcagaggaa attcagagaa
     3601 ggctgggaga tcagagcctg aacggagatc ttgtcatcac gccctacggg agtgaatgtc
     3661 agcagcccgc ttcctatgac ctgagggttg ctgaagatac cgagctgaaa ccgggtatct
     3721 gtaccctcac ccataccctt gaaagggtag agctcccggc cgacatcgct gccacgatcc
     3781 ggacccgttc ttcgtatggt cgtcggggtc tgctggtcac ggcaggattt gtcgatcccg
     3841 gattccgtgg ccaacttacg ctcggcctgg taaatatggg tcaggaagtg atacatctta
     3901 agcggcatga tcgggtcgtc cagctgatcc ttcaccaggt tattggcgcc gggcagacgt
     3961 atgaggggag atatcaggac agcagcggga ttgtcagggc ccggtgattt gatgatacgg
     4021 actgaacgga aatatatcga gattctgaga atattaaaag aacatcagga accgatggga
     4081 gcaaagaggc tctctgagct gatggccgag cgtggatttg tgctgagtga ccgggcagtc
     4141 cagtattacc tccgctatct cgatgaaatg ggtttcaccg agaaagttgg taacatgggc
     4201 cgcatcctca ccccttacgg catgaatgaa acagagagtg cccttgtagg agagaggatt
     4261 gggttcatca tatcaaggct tgagcgcctt gcgttcagaa gcacgtttga tccggttacc
     4321 cagaccggtg atgtggccta caacctttct attgttcctg aagagaggtt tgacgaggtt
     4381 ctcaatgcct ttgatgctgt ggctgctgcc cggtatggtt ttttctcgtc atatcgggtg
     4441 cttgacaatg attcccgtgt ccctgaaggc agtgtgggaa tcataaccgt ttgcagcatc
     4501 acgatggatg gggtcttcca gaacaggggt atccccgtac atatggcatt tggtggcagg
     4561 ctttccatcg aacatggcga acccatacga tttgtcgatc tgatcggata ccgtggaaca
     4621 acaattgatc cgctccagct gttcatctct gcaggattga cttcagtggg atccatgatc
     4681 gccaccggga cgggcattgc gcttgcaaac gtccgggagg tacccgcagc agcagaaagc
     4741 caggtgcgcg agacggtcgc gcagatgcgg aagagtggat tcgtatttcc cgtcatcatg
     4801 gggcccgccc cgttcaacct tccctcagat ccataccgga tttccctcgt ctcttattcg
     4861 gggatgaact tcgtggcaag tgtctttgag aaaaaaatcc ctatcaggac cgagatcggt
     4921 gcaggcaaca tcccattttc aagaattatg ccgcctaatt gatcctttga tacaaaagaa
     4981 ggctcaccct gctatcccgt ggctctggga caggattttt tcatcacgga ttgcccgaag
     5041 tgaatcaacc cgggcaatat gtgaaaaacc agacaggatt acggccactc ctacgaaacg
     5101 actatttttt atcgggtaat cgccggaacg tacctcgaaa ccactgatat tgacatctat
     5161 ccagtgtctg aatgccatat accccctcat gttcagctca tgggacgggc cagcaatgag
     5221 gatcagtgcc ttttctgcat cggcgatgtt acaggagact gacatctcct cgagcaccgc
     5281 cctcttgcca agggcgatga ttcttgccgc tttttcatgg ctttcatcaa cagaatggcc
     5341 tgcgggacgc aatttccgga taaggtcggg agaaactgca gagatgggat cccgtgcaaa
     5401 ccctattgct gccagaccac aaccccgaag cgtattcaca atctcccctg catcaagggc
     5461 gacctctccc acttcagaac cgggacgctc actcacctcc cctgcccgca aaatgagact
     5521 gatccatctt ccaataacgt ggttgacacc ctcatatcct gatgtgattg cttccagggg
     5581 aatgctctga gatcgcttga aagggagcgg taccccctca gtgtgctgga ttccctggag
     5641 atctggatca tttgccacct ttgcgatcca tttctcgtta tcgaacagga gtaccccatc
     5701 cattatcccg gagagggcat ctatctggtt ggctgcctgt atgcggactt cctctgcttc
     5761 agcttcacgt ggaagagtaa acaggccgaa gaccggttca atcatcgttt tccggatact
     5821 caatatgagg gagggagcta gcccggccat agtcccccca agacccagac agattatcaa
     5881 agcatcgata tcaccgtgat caagggactg gagcctggca ataatctcat cattgggcag
     5941 acttgtggga gttttctcat catggttcgg ttcgagtgtc ttcgaaaaaa agaggttctc
     6001 tttggggagt gctctcaggg tggtcaatga tgaggcatcg ctatctatgg caattccatc
     6061 ggtgcagcgt aaggaggagg atttcttgtc atcatcatag atggcgtcag caatccggca
     6121 gcctgccccg ccgataccga caatcaatac tctcaacgca gcaacccctt cctctggttg
     6181 ttatcctttt tccgggagtt cactgaatta ccattggaac cgggttaaaa aaagactatc
     6241 ggactctcca gaaattaatg atttcgatct ccgagaataa gggagcgcgg cggactgacg
     6301 acggcaatga aaataaaaaa aatgggtaat gggggcaatt tttcttctct ccgaatcctt
     6361 ccgagttttg atgactgaaa atgataaggt atttaatttt gtgcccgata tgttacagtg
     6421 aggtgaacta accttgacaa cgtatggaat agaatttgtg cctggcaatg taaatgtcaa
     6481 gcaggtggta aatttctgta aacttgcaga gtccaaagat atcgactttg cgtggattac
     6541 caaccactac aacaaccgtc actgctaccc caccctcgct gccatcgctc aggcaaccac
     6601 aaccctgaag atgggcccgg gtatcatgaa ctcatttact gacaccccgg cagcaatggc
     6661 ctcattcttc tgcacactga atgagatctc tgacggacgc gcagtattgg ggatcggccc
     6721 aggtgacctt tcaacacttc caaaactggc aatcgccgct gaaaaaccag tagcgcgcct
     6781 cgaagaggcg gtcacacaga tccgccggct ctgtgcaggc gaggaagtca agaagtcggg
     6841 aatgcagttc tttgactacg atggtgccaa gctcacgggc gtcactctcc ccggcaagaa
     6901 gggagttccg atttacatcg gcgcacaggg acccaaggtt cttgacctcg ccggaagagt
     6961 cggtgacggg gcgttgatca atgcgtccaa tcccaaagac ttcgccattg ccatcccgat
     7021 cattaaggct gcctgtgaca aggtagggaa gaaaggattt gatgtcggtg cctacaccgc
     7081 catgtcgatt gacatgaacg agaagaaggc ccggaatgcc gccaagatcg ttgctgcatt
     7141 catcgccgct ggatccccgc cggacctcat cgcacgccat ggccttgatg cggcaaaagt
     7201 ggcaacgatc aagggcgccc tcgccaagtt cgatttcaag accgctggtg agaatgttga
     7261 tgacaagacc attgatgcat ttaccattgc cgggaccccg gacacggtaa aacagaaatg
     7321 tgaagacctt gccaaggctg gcgttactca gatcatcttc ggatccccgc tgggaccgga
     7381 catgaccacc tcgatccgcc tgcttggcaa atatgttgtc tgagacaatc cagccggaac
     7441 aacactccct ttttttcata cagtattaat agaaaggctt cgaatgagaa ggtgagacgc
     7501 catgttcacg gtgacagaaa tcaggacggg gacccagccc ctgcaggtaa gagaagaact
     7561 tctgactggt caccgttgca agatctcgcc cggccggctt aaccgtggca ttgatatccc
     7621 ctaccatcct caagatatcc aggaaaactg tccgttctgc agggaacgga tcttctcggt
     7681 cactccggtt tttccggacg ggaaacgtat ctgccggggg gagagcatca ccgtccccaa
     7741 cctctttcct tttggcgagt ggcacactgt caccgtaatc acaagcagac atgttgttga
     7801 taccttcagc ccgggtgaga ttgcagacgc actgtcagga caatcagagt cgctgatgag
     7861 ggcggaaggg tacaagagca taaactggaa ctacctcccc tcttccggag caagcatagc
     7921 ccatcctcat ctccaggggc tggctgaccg gagagctcct gtcaccgccg agcggtatct
     7981 cgttgccggt caacggtatc tcatgaagca gggacgaccc tactgggagg atctccggtc
     8041 atttgaatca ggctcccccc gctacctctt tggtgacgaa ttattctggt atgctcatcc
     8101 ggtacccctt ggcgaaaggg aagtgcggtg cctcctcccc attgctaccc tcgaggagtt
     8161 cactccttac attgaaacgt ttgctgaaga tcttcttcgt gtaattggta tgtaccggag
     8221 gcttggaacg tatgcattca atatttctgt cttttttgat aaaaaaaagg aaaaaaatgg
     8281 attctcctct ttctgttcaa ttatatcccg aataaatccg aatgcttatt cgatgagtga
     8341 ttccgctttc atggaacgcc tccaccttga accggtgatc cttactctcc ctgaagacct
     8401 cgcggatatt ttccggaatg agtactgtta ctcacgtgta aaatcataaa tctgcaacct
     8461 ttgagacatc caataaaaaa acgttcctgc gagcgaaaaa aaagagaagg ggttactcgg
     8521 gcttgctgat cagcttggtc ttcgagacga tgatgccgtc tttcttgtga ggcttcctga
     8581 tgatgccatc gccagccttc tcaatctcac gagcttcgtc gcagagtacc gctgcagccc
     8641 tcatcatctc gtgggcactt gtgacgatcg ggatgtattt ttcccattcc ttcgtcatga
     8701 agcatccctt gacgttcacc atggcaaccg ccgacgcaat ctcgtaagct gcacgggctt
     8761 ttgccagggc gtaggggttg gaaaactccc catcaacagc cttgtctgaa gtgacgatga
     8821 gctttggcat ggcgaggtct gctcccttct ttccagcctt gacctggtcg ataacctggt
     8881 cgatggcatt ctggagctta cggaatgcac cggtgagagc gagcaccttc acgaggttgc
     8941 cgttgtagtc cgccatctcg atgggatcga ggaactcacg gcgggcacca atcatggcgt
     9001 cgcccttcat gatgatgtat ccgaacttgc cgtccttgat ctcggcccac tctttcttgg
     9061 tggtgacatc atcagtgatg aagagggtgg ggattcctgc ggctgccagg cgctcgcggg
     9121 caccggtcgg gccggggagc acgccattcg gtgaaacaac aatcgcgaag tcaggcttcc
     9181 atgcgaccat gttgttgaca accctgtcga tgtcctctgg ctggagcttc gttccgctcg
     9241 tcgccatgaa tgtctgcata tcctctctgt ctgctctctc atcgaggagg agctcggcca
     9301 ttacgccgct ggcaatgttg ccgagtttgg caactccaac tttaactacc atgtatgcac
     9361 ctcattttag tttataacag gttactattc atccatgcgg atataaacgt tttgaaattt
     9421 gattcgaatt caaaaattca gccgtttatg ccgttttctg gtaaaatatg agcataatgc
     9481 agtccctcca tacatctctg ttctcagttg aggggtgtac cggagaatgt taacgaaaag
     9541 tgtttaagtc acctctccca actggtttat catgaaagac gggctgctta ccgatcgcca
     9601 gaaagaggtg ctcagatacc gtaagcaggg gatgacccag caacagattg ctgatattat
     9661 tcgcacatca aaggcaaatg tctgcaccat tgaaaagtcg gcgatggaaa atatcaggag
     9721 ggcgaaggaa acccttgaat tcctttacac gcttgatgca acccacctct gcacgattga
     9781 gtccgggatg gatctcctcg atgttgcccc atatatttac cgtgaagctg aaaatatcgg
     9841 cataaaggtg aaatacgaca cgatctccct tatcaacaag ctgcgtgagg ctactcctga
     9901 gcggttcagg gcacgtcatg tgagagatgc aatcgaggtt tatattaacg aggaagggga
     9961 tctttacttc ggataatcag aacagctggg atcatcggag agcgaatgag agtttcagtt
    10021 ctctgcaccc cgtccggctc caaggaaggt ttatatccgt ttctgtttaa taattatgga
    10081 agcctgtatg attgtcccgg cgaagaccgg gagttcgtat gtggcgggtg ctgtttactg
    10141 tcattgtaaa cggcgcagcc gagaaggagt tgtgattatt cacaacgggc tgacacggtg
    10201 gaactgataa gcgcgggggt ggacgtgttt ctcacgagcc cgcatgtatc agaatggtgg
    10261 tacatccgcg cgacagtcag gatctattcg cgacgaccgg taggaacagg aagaaactgg
    10321 aaggaactga cgtgaaagcg tccgttcacc tctatattct tcctgcaggt cacaggttcg
    10381 ctccgcaaac ggggctctga aaagggctct gaaaagaata cccgtttccg gacagctcga
    10441 ttaccctgag catcacgctc tctcggggca gctgtatgcc ctgcgcatac ccagatcctg
    10501 aacaaggtct gggcatgctg actggtgctg cactgaatcg tcagattaga gaaaggattg
    10561 atgaaaaatg ccgaaaatca acacaccgcg aaaaggatcc ctcgcattta gcccgcgaaa
    10621 aagggcaaaa gggccggtcc ctaagtacca gtcatggccg gaatatgagg gaacaccggt
    10681 gctgcaggga tttgccgggt acaaggtggg gatgacgcat atcgtcatgg tcgatgacca
    10741 caagaacagc cctaccgagg gaaaagatat catggtgccg gtcacggtta tcgagatccc
    10801 tgccatgaag gtcgctgcga tccgtgccta tacaaaagat acgtacggaa agcatcctct
    10861 caccgaggta tgggaagcag ctcctgaccc tgccctcgac cgaagaataa ctcttcccaa
    10921 gacctatgat ggctcagccg cacagaaagc catcctcgat ggaataaaag caggaacggt
    10981 cgcagagatt ttcgccctgg tccatacgac acctgaactg gtatcgggga tccccaagaa
    11041 ggttcctgat atcatggagg tcaggattgc cgggggggct cttgaaaacc ggttcgaatt
    11101 tgcgctgtca cttcttggga aggaagtaac gcttaaaaat gttctccagg ccggatcgta
    11161 tgcggatatc accgcggtta ctaccggaaa agggacacag ggcccggtaa aacgctgggg
    11221 catcaaactc cggaaacgga aacattcccg gggtggaaag aagcgtcacg ttgggaacct
    11281 cggaccctgg aatccgcacc acgtgcgatg gcaggttccg caggcaggcc agatgggata
    11341 ccagcaacgg accgaattca acaagcgcat tcttaaaatc ggggataacg gcaaagaggt
    11401 cacccctgat ggcggatttc tccattatgg cgttctgaac aatccctatg tccttgtaaa
    11461 gggatccgtt ccagggccgg tcaagcgcct agtaaggata cgccccgcca tgaggcttgg
    11521 agaacatgtt cctcgccagc ccgctattga gtttgtgagc gtccagagca agcaggggtg
    11581 agacagcaat gaaagcacag gtaattacaa tcgacggcac aaggaaaggg gacatcgatt
    11641 taccccctgc attctcggaa gaataccgcc cagacctgat caggaaggca gtcattgcgc
    11701 tccagagcac gagaaggcag ccgcacggga catatccgta tgcaggcatt gagtcatctg
    11761 cggtcggatg ggggagcggt cgtggtgttt ctcatgtgcc ccgtatcaag accggatcaa
    11821 gggcagcgaa agttccccag gcgaaagggg ggagagaggc acatccgccg gtggtgcaga
    11881 aggtgcttct cctcaggatg aataaaaaag agaaagcgaa agcattcagg tcagcgcttg
    11941 ctgccagtgc ttctgaggag atcgtgcgat caagggggca tctttttaca ggccagatcc
    12001 ctgttgtgtt cgaggattcc ttcggagaac ttggcaggac ttccgaagtc atctcagcgc
    12061 ttacggcagc cgggctttac caggacatcg aaaggtccaa gagttcacga aaagtcaggg
    12121 ctggccgggg aaagatgaga gggcggcgtt acaaacagag gaagagcctt ctcatcgtca
    12181 ccgctgaaaa acccctcagg gctgcccgga acctggcagg ggtggacgtg gtcacgctcg
    12241 agcagctcaa cacggaactc cttgcacctg ggactcatcc tggccgtctc acggtctaca
    12301 cagagagtgc aatctcacga cttggggggc agtaagatgg tgctccgaca tccgtatgtg
    12361 acagaaaagg ccatgatgac tcttgaaaaa gagaacaaac tccagtttct cgttgacaaa
    12421 gacgccacga agaagcagat aaagagagag atcgagaaga ccttcgaaca ggaggtcagg
    12481 ggtgtctcga ctgtcatgac catgcacggg gagaagaaag cgatcatcag ctttgccaac
    12541 gaaaaggcag ccgaggagat actcagccgt cttggcatta tgtaggtggt ttgaatggga
    12601 caccgtatta caacacagaa cagaggaaaa ggaggcccgg cttaccgggt gccgtcccac
    12661 cgctacaagg cagcacttcg tcatgccagc agcggcacta caacggtaaa ggggatcgtg
    12721 gtagatattg aacacgatcc ggcacgacac actcctatcg cgctcgtgaa actcgaagac
    12781 ggatcgagga tgtacatgct cgtcactgaa ggaattgggg taaatgatga ggtgagctgg
    12841 gggcccgagg gagaggttcg taatgggaac acccttcccc tgtctgcaat cccaaccgga
    12901 gcatatgtat gcaatatcga ggcaaggccc aatgatggag gcaagttcgt ccgtgccagc
    12961 ggcgtgcagg caattgttgt ggataaaact gcggaacggg ttggcgtcca gatgcccagc
    13021 ggcgttacca aatggttcaa tgccagctgc agggcaacca tcggaatagt tgccggcgga
    13081 ggacgcggag agaaaccatt cgtgaaggcc gggaacaagt tccacaagat gaagaacagt
    13141 gcatccaact ggccccgggt acggggggta gccatgaacg tgatcgatca tccgttcggt
    13201 ggcggtggtc accagcatgc gggccggcca aagactgttg caagaggaac atcccccggg
    13261 aggaccgtcg gccatgttgc cgcacgaaag actggacggg gtaagaagtg agggtttgac
    13321 atggcaaaga agacacagaa gaggttgcca agacggaggg aagagtttac ctaccgtgga
    13381 ttccggctcg acgatctcgt gaagatgggt acggctgacc tcctgccgat aatgcctgcc
    13441 cgggtccgcc ggaaagtgag caggggcctg acccgcggag aagagcatct cctctcgagg
    13501 ttcaaaagcg gggatgccag gatcaggacc catcttcggg acatggtgat catgcccgat
    13561 atgatcggaa aagaggtgga gatttacaac gggaaggaat tcatgaaagt ggagatccag
    13621 cccgaagcga tcttccatta cctcggggag tttgccctga cacgaaggaa ggtaacacac
    13681 gggagtgctg gaattggtgc aacccgttca agtaagtatg tcccgctgaa gtgagaacca
    13741 tggcacgcac aggatattca actgatatta ccggggatac ggtggctcgg ggaaaggcaa
    13801 acgaacttcc gatatccccc aaacatgcca tggagattgc caggttcatc cggaacaaaa
    13861 agaccggtga agtggtctcc tatcttgagg atgttgttgc agaaaagaag gcaataccct
    13921 tccgcaggtt caaccacaag gtggctcaca agcgagggct ttcaggatgg gatgccggcc
    13981 gatacccgag aaaggcatcg aaagcgtacc ttcggctgtt acactcggtc accaaaaatg
    14041 ccgagtatct tggccttgac accgaacggc tcgagatcgt acatgttgct gcaaacaggg
    14101 gccggggatt tgaggggatc ttcccgcgtg cgatgggaag ggcctcaccc aaacggcgtg
    14161 aaacggtaaa catcgagatt gtggtaaagg aagtgcagta aatggcagtc gagagaaaat
    14221 ttgtggccga aggagcgagg agagcccggg ttgaaaaaca cctggtcaag gagctcaaga
    14281 gggctgggta tggaggtatg gacctgcaac ggactcccct cggcacccag gtaaccattt
    14341 acgccgagaa gccaggaatc gtgattggga agggaggaaa agtcgtacgg cagctcacac
    14401 aggaccttgc gaccgatttc ggtgttgagt ccccgcagat cgaggttcag caggtgccaa
    14461 accccagttt caatgcccag atcatggccg agcgcctggc taacgctctg gaacgcggtt
    14521 ggtatttcag aaaggccggg tcgagtatta taaggagagt tatggaatcc ggcgctctgg
    14581 gatgcgaggt catcatcgcg gggaaactga cgggagcccg tgccagggtt cagaagttca
    14641 ccgaagggta catcaagcac agcggagagc cggtaaacac catagtggag aagggctttg
    14701 ccgtggcaat caagaagctg ggggtgatcg gtgttcaggt gcgaatcatt cctcctgaat
    14761 ccaagcttcc cgatcacttc gagatgatcg ttcccaagca gaaacttgtc cgaaaggaag
    14821 agattgctat cactcctgtc ggtgaagaga ttgaagagga ggatctggca atggatctcg
    14881 atgagtttga tgatgtggaa actccggagg atcggtaatg gccattcttc gtgcaagcga
    14941 tatccggcag ctctcagatg tggaattgca ggaacagatg gagaaactca ggatagagct
    15001 tatccagcac aacggaaagg tcagtgccgg aggatccacc gaaaatccag ggagaattcg
    15061 tgagctccgg cggacaattg cccgtatgat gactgaacag aatcacagga gaacagtatg
    15121 atctctgccc ggaacatcct gcgccatgaa cttattggcc tggttgtttc ggtagtggat
    15181 gcatcaaatc cccttcaccg tggaatatca ggccagatta tcgatgagac cagaaacact
    15241 ctcctcatcc gcacaccgga tggcgacagg aggatacaga agcagtacac ccgcttctgt
    15301 atcagtattc ctgatggaac ccgggttctc atcgatggat ctgcgatcgt gtcatctccg
    15361 gagaagagga taacgcagta tgcgaaaatt cgaggtaaat aatggcgaaa aatattggat
    15421 tgggcgtcct gccacctgaa caggcatgca gtgacgcaaa ctgcccgttt cacggcagtt
    15481 taccggtgcg cggccaggtg atcaccggta aagtcgtgag cgatcgcatg atgggaacgg
    15541 tagttgtgga gcgtaactac ctccatttcg tgcgaaagta taaccggtac gagaagagaa
    15601 gctcgaggat tcatgctcac aatcctccct gcctgcaggc aaaagtcggt gacgatgtga
    15661 agatcgccga gtgcaggccg ctctcgaaga ccacgacctt tgtagtggtg gaggtacaga
    15721 ggctatgaaa gccaaacagt caaagacgcc gcgatccctc tcaacaggga ccaggatgat
    15781 ctgtgccgac aacaccggag cacgtgaagt ccagattgta tcggttttcg ggtatcacgg
    15841 ggtccgccgc cgccagccca agatgggtct cggtgacctt gcgacggtta cggtaaagaa
    15901 gggaaccccc gatatgcgga gaaaactcgt cagagccgta gtaatccggc agagaaaaga
    15961 gataaaacgc ccgaacggtc ttcggattac gttcgatgat aacgccgtgg tggttgtcga
    16021 tgagaagaac gagccacggg gtacagagat aaaaggtccg gttgcacggg aagtcgctga
    16081 gcgctttcca aagattggat cgatggcaac gatgattgtg tgaggtgtgc ggaatatggt
    16141 aagaattgag agtacacaac caagaaaaca gcgcaaggcg cgctataatg cccccgcgca
    16201 cctccgcgga aaattcatca gtgctcccct caccgatgaa ctcaggacgc aatacaagag
    16261 aaggaccgtc cgggtcgtga agggtgatac cgtaaaggta ctcaggggag atgctgcagg
    16321 gacggagggg ctcgtcgatg atgttgatac cggaaggtcg ctcctgatta tccagggagt
    16381 ctcgattccg aaagcggacg gaacagaagt tccacgaccg gtcgatccct ccaatgtgca
    16441 gatcaccaag ctcaatctga aagatcccaa acgggctgaa aaaatggcgg aggtacgctg
    16501 atgtcagatc acctgaaacg tctgcgtgca ccagactcat ggcacattgc caaaaagacc
    16561 agcacattcg ttgcaaagac cgctcccggc cctcataatg ccaatgccat gccgatcgcg
    16621 gtctggcttc gtgaccacat gaactttgcg cgcaatatga aggaagtaaa gcagattctc
    16681 ggccagaatg atgttattat caatggtcgg ccctgccgcg atccacggat gggaatcggt
    16741 attttcgata tcatctctct tccaaagatc gggaaacact atcggatcct gcgcgacaag
    16801 aaaggacggc ataagacgat cgagataact gccgaatcgg caaagacacg ccttgccaag
    16861 gtgaggaaca agacaattat ccccggcgga aaagttcagt tgaaccttcg ttttggagca
    16921 aatgttcttg gtgacaacca gtataagcct ggcgactctg tggtcatctc cctgcagccg
    16981 gaagatcggt ttaccatcac agaccatttc ccatttgcgg tagggaacat ggcaatggtc
    17041 attggaggga gacattcggg acgtatcgcc cggataaccg ctatagaaaa agtaccgggc
    17101 agtgttccaa accgggtcct gctggccgat gagaaagccg ggactacctt cgataccatc
    17161 gatgcctata tctacatggt aggtcgcgag accccagctc ttgcagaatg ggggattgag
    17221 gaatgaatcc gatgcgcgaa gtctttgttg acaaggtggt cgttcacatg ggcgtgggag
    17281 agagcgggga caagctcacc aaagcggttg atatcatgaa gaagatcacg ggcaacaatc
    17341 ccgtaaagag tgtggcgaaa aagaccaatc cggcattcgg aatccgcaag ggtgccccga
    17401 tcagctgcaa ggtcacgctc aggggagaga ctgcaggtga ttttcttgac acagcgcttg
    17461 ctatcatcga aaggaaagtc agccgtgacc agtttgacaa gagcggaaat ttctctttcg
    17521 ggattgaaga gcatactgat ttccccggga tgtcctatga tccccagatc gggatttatg
    17581 gcatggacat aaacgtgatc cttgccagga aaggtgtccg tattgcccgc cgcaacatcg
    17641 aacgccaccg tcttccccaa aagcagatgg ttcagcctga ggatgcgatc gcattcctta
    17701 aaagtcgcta tcaggcggag gtggcatgat gggcggagag aagaccaaaa agttcggccg
    17761 cggggcattt gcatgcaaaa tctgcggcag gaaacaaggc cttgtccgca gataccatat
    17821 tatgttctgc aggcagtgtt tcagggaatg ggcccagaag atgggattca aaaagatgaa
    17881 ttgaggagga agggcacatg ggaagattaa acacaattgc agatgggatg agtgcgctga
    17941 agaatgctgc agatggcggc aaacctggcg tccttatcga gccggcaggc aaactcctcg
    18001 gggccatgtt ccgtatcatg caggaatcag gatatatctc cggatttgag tatgttgatg
    18061 acggtcgcgg agggcaattc ctcgttcagc tgaatgggcg gatcaataag tgcggtgcaa
    18121 tctcaccccg gtattccgta ccattgaatg agcttgagta ttgggagacc cagtatcttc
    18181 ctggaaagaa tttcgggatt ctgatccttt ccacctcaaa aggggtcatg acccatgaac
    18241 aggcaaggaa ggaaggggtc ggtggagaac ttctcgggta tgtgtattag aggtgagaga
    18301 gcatggcatc ggaaagaaaa gtaaccatac cagaaggagt gaaggtcgag ctggaaggga
    18361 ccttgctgaa agtgaagggg ccgaaaggtc agcttgaacg gatcgtaagg ttcccccaga
    18421 ttactaccgt tttggcagac aatacggtga tcttctccac taattcagat cgtaagagtg
    18481 tgattgccat ggtgggcact tttgctgcac ataccagaaa tatgtgccac ggcgtcaaca
    18541 ccggattcga ataccgtatg aaggtggtct acagccattt tcctatccag ctcaagctgc
    18601 agggtaataa acttgagatc aataatttcc tgggtgagaa aaaggccagg tatgccacca
    18661 ttgtcaatgg cgtgacagca aagataggaa atgacgagat tatcctctcc ggaattgata
    18721 aagagctcat tggcaatact tcggccaaca tcgaacatgc gaccaagatc cggaaccgtg
    18781 atccccgcat tttccaggat ggaatttata tcgtcgagag ggcgtgattg aaatggccga
    18841 cgagacaaaa aggttaatcc aggtccgttc cgacaaatca gcatcattca agcgggacgg
    18901 ggcggggaaa aagaagcagc tctccgaatc ctggcggatg ccgcgcgggc tccattccaa
    18961 aaagcggagg cagaagaaag caaaagggcc gctcccgaca ccagggtatg gcagccccat
    19021 cgaggtcagg ggtatgcacc cgagcggata tcgtgaaatc cgggtcttta ccccaggtga
    19081 tcttgcagga ttgaatcctg acgaagatgc tatccgtata ggcggaaccg ttggaaacaa
    19141 gaaacgaatg cttatacagg atcaggcggt tgcagcaggg ctgaaggttt tgaacgcgag
    19201 ggatatcagg cccaaggagc aggaggcgaa gagcgatgac tgatattgca aaccagcggc
    19261 gtctcgcagc atcgcttctc ggttgtggta tcaatcgcgt atggtttgat gccgaacgga
    19321 tcggcgacat ccagaatgcg atatcgcgag aggatatcag gggacttatc ggagagggtg
    19381 tgatccgggc acgtcaggtc cagggcaaca gccgtggaag agcccgggcg cttatggcga
    19441 aacgttcgta tgggcactgc aagggacccg gcagacgaaa aggggctgcc ggggcacgaa
    19501 atccttcgaa aagggagtgg atacagaaga ttcgggcgat cagaaaaact cttgcagaac
    19561 ttcgcgagag cggagagata gcacctcacc tctaccgcac cttgtatcgg aaggctgccg
    19621 gagggcagtt caggaacgta gcgcacctga aagctcaaat cgggatcatt tcagggagga
    19681 tgaagtaaca tggcaacggg acctcgttac ttcgttcctt tccggagacg caaggaaggg
    19741 aagaccgatt attacaaacg ggccaaactg gtggtctctg accgcccacg gatggtcgtg
    19801 aggaagacca accggcagat catcatccag atggtattgg cagagatgga tggtgaccga
    19861 actctggtga gtgccagttc caccgaactg gcgaactatg gatacaatgg atcgacatcc
    19921 gcactcccgg cagcatacct taccgggatg ctttttgcgg taaaggcact gaatgccgac
    19981 cagcaacgag ccgtccttga cataggtctg caccgtgcaa caccggggaa cagggtcttt
    20041 gccgcgctca aaggagctgt tgctgccggc cttgatgtac cccatggaga acaggtgctc
    20101 cctgatgatg atcgggtaaa aggtgcacat atcgcagcgt ttgcacccga acgtgcaggg
    20161 gatctggttc ggaacgtcga tgagacgatt gacgccataa tgaaggagtt gaggtgagat
    20221 gccttacgag aagacagaat gggttccgct taccggccta ggaaagatgg tagcctcggg
    20281 agagattacc agcatcgacc aggtgctgga gagcgggcgt cccatcaagg aacctgagat
    20341 cgttgatgca tttctcccag atctggaaga tgaagtgctc gatatcgaga tggtccagcg
    20401 catgaccgat tcagggcgca gagtcaaatt cagggcagtg gttgtcgtcg ggaaccgtaa
    20461 cggctacatc ggattt
//
LOCUS       DAOOCO010000010        19273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2511_length_19273_cov_5.334946, whole genome shotgun sequence.
ACCESSION   DAOOCO010000010 DAOOCO010000000
VERSION     DAOOCO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 19273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19273
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_19273_cov_5.334946"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK154_01280"
     CDS             complement(<1..285)
                     /locus_tag="PK154_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 1 protein"
                     /protein_id="HPW09727.1"
                     /translation="MMSFTLQRVRKQYLDGTHRVKSPDETLVSVSPLMEMIGVEEVKD
                     ISPSDRIGIPCFSAFRPRAARGGVRYHAGKGKDPVQAKVSAMMEAVERYSA"
     gene            339..632
                     /locus_tag="PK154_01285"
     CDS             339..632
                     /locus_tag="PK154_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HPW09728.1"
                     /translation="MNAEELVIGMQVRYPRTGTSGRVTRVEQQHGDLFAELDSTGLLY
                     KIVELVPAASTKGKEEKSREKDLESIKKEREFLSDEAFREALSHTDQSCEGGG"
     gene            complement(629..1051)
                     /gene="nikR"
                     /locus_tag="PK154_01290"
     CDS             complement(629..1051)
                     /gene="nikR"
                     /locus_tag="PK154_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPW09729.1"
                     /translation="MTIDTELSRIGISLPKNLLDKFDEIINYRGYSSRSEGIRDAIRS
                     YITYYKWMSDVKGERQGVITMIYDHDQRGLLSTLTDIQHEFMGIIQATLHSHVSHDRC
                     LEIILIRGEGADLKTLAERLMSQKGVESVKLTTIPIND"
     gene            1264..2499
                     /locus_tag="PK154_01295"
     CDS             1264..2499
                     /locus_tag="PK154_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HPW09730.1"
                     /translation="MRDVTIPGVNIGVVGHVDHGKTTLVYGLTKAWTDRHSEEIKRGI
                     SIRLGYADATFYRCEGCAGSDSFSTTPECPICGTKGVPFRSVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANEPCPQPQTKEHLMALELVGIKRIVIVQNKIDVISQKDALKHY
                     EQIKAFVKGTVAEGAPIIPISAQKSINIGAVIEALDAVIPEIERDPGANPVMLIARSF
                     DINKPGSNWRDVKGGVIGGSLIRGVFKEGDDIEIRPGRQIQAENKIRWEPITTKITTI
                     NAGSKKVPEAGPGGLLGIGTKLDPALTKSDALAGQVTGHTGALPPVWDRLRFQVTLME
                     RVVGAMSELSIEPLKHHEPLMLSVGTAVTVGVVTSARKDLAEVTLKRPVCAEPGARIA
                     ISRQVGARWRLIGMGVLTE"
     gene            2463..2876
                     /locus_tag="PK154_01300"
     CDS             2463..2876
                     /locus_tag="PK154_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HPW09731.1"
                     /translation="MASYRDGRPDRVTVLIDANALMAQLQFRIDIFAGLTECIGAFEP
                     VLLDCVRDELRGLAASHGKEGSSARSALILAERCTIAETGMYGGSVDEKILYYASTHG
                     CMVFTNDRDLRTTLLAQGIPVISLAGKQRLEVYRN"
     gene            2884..3477
                     /locus_tag="PK154_01305"
     CDS             2884..3477
                     /locus_tag="PK154_01305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HPW09732.1"
                     /translation="MYYRIKLADKVRVPPHRLGEELRKVILDVLQEQLEGSIDKEIGI
                     FIAITKVLDVGEGEIVPGDGAVYYDVNFESLVLRLSLQEVIEGMVVETTSFGAFISLG
                     PIDAMLHVSQISDEYINYDEKNARLICQESKRYIAVGETLRVRVVTLSLNEREPRDSK
                     IGLTMRQSGLGTAKWLDEELAKEKEKEKEKGGERGGR"
     gene            3464..3667
                     /gene="spt4"
                     /locus_tag="PK154_01310"
     CDS             3464..3667
                     /gene="spt4"
                     /locus_tag="PK154_01310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HPW09733.1"
                     /translation="MAGARKKLVHVCRDCHRVVEGESCAVCGSANLSSDWTGYLVIID
                     PRHSEVAKKMNISLPGRYALKVR"
     gene            3670..4182
                     /locus_tag="PK154_01315"
     CDS             3670..4182
                     /locus_tag="PK154_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HPW09734.1"
                     /translation="MLLLPESRRYLFRQPFGVLCPDMNCVLPLIEGKVVYTVGDVVTH
                     HLIQQGIVPAIAIIDGHTRREPCNRSPAVFRKRCGARNPAGTLTIELRNTLDAAVGDP
                     DTEVLIMVDGEEDLAVIPLILSAPEGVVVLYGQPGEGVVVCEVNEAAKEKAIALLSCF
                     VDDGKDLLPG"
     gene            4238..4534
                     /locus_tag="PK154_01320"
     CDS             4238..4534
                     /locus_tag="PK154_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HPW09735.1"
                     /translation="MDFEVTRDTRNELLARREVDFRLRFEGPTPSRMQVIGKLAAGLN
                     VSEKLLVLDTMKTSFGKTELTGMARVYDTEEQKSKTERAYLSTRGMPKPKEEGA"
     gene            4534..4713
                     /locus_tag="PK154_01325"
     CDS             4534..4713
                     /locus_tag="PK154_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HPW09736.1"
                     /translation="MAAKKGKAPSVRRASCYKTEGGKAILAKKFCPRCGPGVVMADHK
                     DRMACGKCGYTEFKK"
     gene            4720..6297
                     /locus_tag="PK154_01330"
     CDS             4720..6297
                     /locus_tag="PK154_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HPW09737.1"
                     /translation="MPVFGRILGIEGTAWNLSAAVFDNDLITLHSKPYRPPTGGIHPR
                     EAAQHHASEMKAVISKVLSEPDGITGVAFSQGPGLGPCLRTVATAARSLALGLGVPLV
                     GVNHCVAHIEIGRYATGCSDPVVLYASGANTQVIGYLNKRYRIFGETLDIGIGNAIDK
                     FARSKGLPHPGGPAIEELARKGRYVDLPYTVKGMDLAFSGLVSAARECRAPIEDVCYS
                     LQETAFAMCCEVTERALAHTGKEEVLLVGGVGANSRFEEMLSQMCADRGANLFVPERK
                     YLGDNGAMIAYTGKIMLESGYSVPPEESYVNPSFRSDQVEVTWRHDDPGERIRDKPLD
                     QITRATGAEAEVLLGELFVEKRRVRKLYRQQELNDRLIAERTRAEARLIASSRKEGVP
                     TPVIQDITGTTIRMERIYGSLLKDSLSPVHLEEAGRTVGTLHTAGIVHGDLTTSNLIM
                     REGRCVLIDFGLSLASSEIEARGVDLHVLFQTLESTTDNFSELKEAFTRGYEHTFSGA
                     DEVLAREREIEKRGRYL"
     gene            6294..6851
                     /gene="rdgB"
                     /locus_tag="PK154_01335"
     CDS             6294..6851
                     /gene="rdgB"
                     /locus_tag="PK154_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPW09738.1"
                     /translation="MKVAVVTSNRHKAAEIADYFGTLVEVMHVTMEIPEYRHDDVGEI
                     AYKKAEYAYHALRCPLLVDDTAFSIEALKGFPGPYAAYVLSTLGNHGILKLLEGENNR
                     NAYFETAIAFATTQGTIHVFRGRIDGTIVSPRGDSGFGYDPIFEVEGKTLAELDRSSK
                     NQISHRARALSLLKAWILAGDPGMP"
     gene            6909..7055
                     /locus_tag="PK154_01340"
     CDS             6909..7055
                     /locus_tag="PK154_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HPW09739.1"
                     /translation="MARFPEAEARLLNVKICMRCNARNAIRATSCRKCGYKNLRPKNK
                     EKKA"
     gene            complement(7060..7710)
                     /locus_tag="PK154_01345"
     CDS             complement(7060..7710)
                     /locus_tag="PK154_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HPW09740.1"
                     /translation="MEKVDPEALADVAYGLFEVMLNTGLRERGPYLFELVEKGIDFEA
                     QFLEIFRKFCDDYPQLGEALIFRFRSPHVIYHAIREGEGVIPSRTTRMYWIVQDAPDV
                     KPDAIEDELAGKWLIFLPPERVDEAWILVRDATCRNELGISAKVSTAKPNPDSRDSMK
                     VIYIYTPDWRDEQEVMRVRERLRELGFADRIGYKRNIETFKGEYSQKGKRVTFYSA"
     gene            complement(7757..8524)
                     /locus_tag="PK154_01350"
     CDS             complement(7757..8524)
                     /locus_tag="PK154_01350"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HPW09741.1"
                     /translation="MELDERIITRAIIESELTALLDCIETDVAIVGGGPSGLAAASFL
                     GEAGLAVTLIEKKLSVGGGMWGGGMMFPRIVVQESARRLLDHFRIHYHSYTEGYYVAG
                     AVEAVSKLTSAACDAGVEFLTLMTVEDVMIRSENRLSGLVVNWTPVGIAGLHVDPLTL
                     WCRYTIDATGHDASVARIVGKKTDQVFVKGEGFMWAEKAESMIISHTKEIFPGLIVAG
                     MAANAVFGETRMGPVFGGMLLSGEHAASLVLRELGTR"
     gene            8772..9656
                     /gene="htpX"
                     /locus_tag="PK154_01355"
     CDS             8772..9656
                     /gene="htpX"
                     /locus_tag="PK154_01355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPW09742.1"
                     /translation="MKPWKRDWGLTGRIWLTMFLLFLVYLAFIAVLSMLGIGLPFLVV
                     IVFLMAFLQYFFSDRLVLWTTRSRVIEQEEYPELHRIVERCASEAGIPKPRIAIMQTP
                     VPNAFATGRSPKHAVVAVTDSIMRLLTPRELEAVIAHEISHIRNRDVLTLTIASFVAM
                     IAALVMNNFLFAAMFGRRDSGNAWIIAGIVAVVVYFVAQLLIMALSRYREFAADRGSA
                     YITGSPRDLISALGKISGRMEAVPPQQKQAVETANAFFIIPALSGSTLLELFSTHPAL
                     EKRIAALEQVELQLKGRA"
     gene            9766..9836
                     /locus_tag="PK154_01360"
     tRNA            9766..9836
                     /locus_tag="PK154_01360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9798..9800,aa:Gly,seq:tcc)
     gene            9840..9913
                     /locus_tag="PK154_01365"
     tRNA            9840..9913
                     /locus_tag="PK154_01365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9874..9876,aa:Val,seq:cac)
     gene            10258..10713
                     /locus_tag="PK154_01370"
     CDS             10258..10713
                     /locus_tag="PK154_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW09743.1"
                     /translation="MTPEQYRAILSKAIEREVESYTYYRTISDKVKDAYLKKIFADLA
                     GDETKHREFLQDLLAKEPKKLKISASKDYKIADSVKAPTLSADLKPLDGIVLAIKKEL
                     EAMQMYTQLADAQTDPEQKNLFTDLAAMERGHKARLEDIYTNMAFPESW"
     gene            10978..11835
                     /locus_tag="PK154_01375"
     CDS             10978..11835
                     /locus_tag="PK154_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW09744.1"
                     /translation="MHNIARSSRVNAVLALAAISILVAFLIPVQVMGDSPSEIASTFI
                     QKGDDLTLRKEYHDALEAYESAVASDPYNSNAWNKLGVAHMNTGRYQDAVEAFERSLA
                     IDPYNQKAWNNKGDALAALGEYEDAILAYEQALRLNSNDHYALLRKGISLQQAGFSAE
                     AMLVYQEVVSLAEKEVRKHPDYARFDGELWTNKGEAFYRLGRYEEALEAYTTALTINP
                     KYDRAIQGKIHAEEEILRARGAPPTPSPVIEEKGVDIGLTNLSMSLPVLLGALVIALF
                     FLRLRPPCH"
     gene            11858..12979
                     /locus_tag="PK154_01380"
     CDS             11858..12979
                     /locus_tag="PK154_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HPW09745.1"
                     /translation="MFYHLILTDKCNLCCRYCRGNESEVFLPLEGDSGIDLDLPLELA
                     YDLTDLYSFLERDPSPCITFYGGEPLIRADLIREIMDDAPHVSRFMIQTNGLLLDTVG
                     QEYLNRMETILVSIDGAETLTDYYRGSGTYRRIISQVNTLLSEGYQGELIARMTVSRK
                     TDIRSAVHHLAFNHECPFSSIHWQIDADFSGNPGIFDFLGWLEQSYKPGIRRLAGDWV
                     KIMEEEGRVTRWYPFLQTTEDLLTGRTSRLRCGSGYANYTIMTDGHIGPCPIMTGMRD
                     YYVGHIRTADPCSLPEVGIREPCPDCEIYGFCGGRCLYASVVRPWSAEQKELICSAVK
                     ALFCDMSDILPRIRTCIESGRIQMADFSHTRFNGCEIIP"
     gene            complement(12991..13470)
                     /locus_tag="PK154_01385"
     CDS             complement(12991..13470)
                     /locus_tag="PK154_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPW09746.1"
                     /translation="MQIQIVAIGKIRESFIREGIGVYETRLCSANKITFTDLPEERIP
                     SNSSDTEKRLAKEREGRKLLAAAPDTHILVALDPGGTMLSSRELSDLLAEWELKGRGK
                     ISFFIGGTYGLSPEVISNADLVLSLSRMTLPHELVRLMLIEQIYRAECIRRGIPYHK"
     gene            complement(13903..15486)
                     /gene="sepS"
                     /locus_tag="PK154_01390"
     CDS             complement(13903..15486)
                     /gene="sepS"
                     /locus_tag="PK154_01390"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HPW09747.1"
                     /translation="MRFNPEEYRKAARDHFEQAWHKGPLVVTPPGREQEYPRLQYRKA
                     LPHPIFEMVGRLRETYLLLGFEEACNPIIVEEQEVYRQFGPEASAVLDRVFYLGGLER
                     PNQGIGKSELDGIAAITGKQVSAEEEDRLRELLQSYKKSVIDGDDLIHATSHALGIDD
                     GATVEIYEKVFSAFRSLEPRSSRNTLRSHMTSGWFMTLSHIWEKRPLPLRMFSIDRCF
                     RREQEEGPTRLMSYHSASCVIAGDDVTIEDGKAVSRALLSSFGYTDFRFQPDEKRSKY
                     YMPGSQTEVYARHPVHDWVEVATFGMYSPSALGEYGIGIPVMNLGLGVERLAMIAYQS
                     DDVRRLTFPQFFPIDLTDFEIARAISFREEPKSPIGKNLARSIISAAESHGTEPGPCS
                     FLASESEIEGRPVRVYVEEPESGTRLLGPACMNRIYVLSGSVMGVPATEKWKEVREAG
                     VDSGITYLAATAALAAARIEEGVRCGRPVTVQVKMAKLPSDINIRIEDYAMRYITDQK
                     KKVDLRGPVFFTVRSELVT"
     gene            complement(15483..16376)
                     /gene="twy1"
                     /locus_tag="PK154_01395"
     CDS             complement(15483..16376)
                     /gene="twy1"
                     /locus_tag="PK154_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HPW09748.1"
                     /translation="MPSSPCKALRGQGYQFFSPESSAAVKPCLWCKKALKGGAQCYKH
                     QFYGIQSHRCIQMTPTLRCNHRCLFCWRSFEHEYPGERECTPGEILAGLYDLQKRALS
                     GYKVSPYVTSERFAEALRPAHIAISLSGEPTLYSHLPELIDLLKTQGFTTFLVSNGTN
                     PDVISQCFPFQTYISLPAPDQETYLSICRPSADYWDRICRSLTHLSTRRSAVRVTLVR
                     GLNDHSPEKYAALFEKSNARYVEIKGYMYLGYSRKRLNREHMPDHAYIKEFAERIAEY
                     CDYRITNENAPSRVILLENTA"
     gene            complement(16357..18021)
                     /gene="argS"
                     /locus_tag="PK154_01400"
     CDS             complement(16357..18021)
                     /gene="argS"
                     /locus_tag="PK154_01400"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW09749.1"
                     /translation="MYLEIYGTIERALAAATGIPHPTLTDGGIHADFATAISFERAKA
                     LHRPPVQIAEEIATALRADSSLEGITIETKGPYINFQTGPTFLKSSVEAALQPGYGTL
                     LMKGGRVVLEHTSANPNGPLHVGHIRNTIIGDTLARLFRKAGYRVEVQYYVNDMGRQI
                     AIVAWGFSHLDGARKDGEKGDHHVARVYIAANREIERDPGIVPEIDLLMQQVEKGDPK
                     TAVLFRNAVSECLEGFKVTLALLSARHDRFVWESDFVRNGDTERLLNRVRRMEECHED
                     GKLWLDLSNEGFEKEYILRRSDGTSVYAARDLAYHIWKGRNFDRLIDVLGADHKLIGA
                     QLIATLKLIGEKAPEIVHFEFVSLPEGSMSTRAGKFVSADELIEEVTRRAYEEVTLRR
                     DDLDENTKQEIARSVAIAAIRYDIVKISPEKSTVFNWKEALDFERQSGPYIQYAHARA
                     CSILDKAGGFDTAFEFTMESEIALAKHIAKFPFVIRNAVQDLKPHLVATYARELADFF
                     NVFYQFEPVLKSKGVTRQSRLTLVKATKNTLAETLGTLGIDALVTM"
     gene            complement(18021..>19273)
                     /gene="prf1"
                     /locus_tag="PK154_01405"
     CDS             complement(18021..>19273)
                     /gene="prf1"
                     /locus_tag="PK154_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HPW09750.1"
                     /translation="DNARRRYEFKKTLEKLQAKQGSGTELISLYIPPDKQIYDVTAQL
                     RDEFGQCANIKSKQTRTNVQSAISSILSRLKYYKSPPSNGMAVFCGTIQMGGDRSDLE
                     CTIIEPPEPLNLYIYRCSSNFELEPLKQMLEEKMVYGLLVLDRREAYWGFLRGNRIEP
                     IGGVTSTVPGKQRKGGQSSARFQRLREIAIDEFYTKIGERASAIFLAEKDFFERFQGV
                     LIGGPSPTKEEFEAGKYLHHEIQKRIIGLFDVAYTNEDGLSELVDAAKDALKGQEVVK
                     EKEVMERFLKELVKDAGLASYGEESVRQNLELGSVDVLLLSSRLRKSRVKITCQSCGH
                     SEERTVSLEPGKTVEDILTHSCRLCTAPLIVESEVDIVEELTNLADQTSTRVEIISDD
                     FEEGAILYNAFGGIAAILRYRTGS"
BASE COUNT         4560 a         5004 c         5038 g         4671 t
ORIGIN      
        1 tgcactgtat cgctccactg cttccatcat tgccgatact ttcgcctgga caggatcctt
       61 gcccttgccg gcgtggtagc ggacacctcc ccgcgctgca cgggggcgga aggccgaaaa
      121 acaggggatc cctattctgt cagacgggct gatatccttc acctcttcaa cccctatcat
      181 ctccattagg ggactcactg aaacgagtgt ttcatcggga gattttaccc ggtgggttcc
      241 gtcaagatac tgcttcctga ccctttgcag cgtgaagctc atcattcgtt atggcataga
      301 aaatatcttt atagtatcct tatgcctcaa tgttaggtat gaacgccgaa gaactggtaa
      361 tcgggatgca ggtccgctat cccaggaccg ggacaagcgg cagggtgacc cgtgtcgagc
      421 agcagcatgg tgacctgttt gctgagcttg acagcacagg tctcctgtat aagatagtgg
      481 aactggtgcc tgccgcttcg acaaaaggga aagaggagaa gagccgggag aaggatctgg
      541 agagtatcaa aaaggaaagg gagtttttat cagacgaggc cttccgcgaa gcgctctcgc
      601 atacggacca gagctgcgag ggtggaggtt aatcgttaat gggtatcgtc gtcaacttga
      661 ctgactcaac tcccttctgg gacatcagtc gttcagcaag ggttttcagg tcagcccctt
      721 ctccccttat gagaataatc tccagacatc ggtcgtggct tacatgggag tgaagcgttg
      781 cctgaatgat ccccatgaat tcgtgctgga tatcagtcag ggttgagagc aacccccgct
      841 ggtcatggtc atagatcata gtgatgaccc cctgacgctc ccctttgaca tcagacatcc
      901 acttgtaata cgtgatatag ctccgtatcg catcccggat cccttccgaa cgggaggagt
      961 agccccggta gttgatgatc tcatcgaatt tgtccagaag gttttttgga agcgatatcc
     1021 caatcctcga gagctctgta tcaatcgtca tcagaaggta cctcggataa tgagatctgc
     1081 acagatcctc cacttaatcc ttccggccca tcattggtaa agtaccactt tttcagaaga
     1141 tttccagaaa atgcccatta ccctctccac ctcccatgga ctagtttcat aaccatacgg
     1201 cgatccatga ccggtttgta taagtaggtt cattcaatat tacataagga ggtttgatcg
     1261 catttgcgtg atgtaacaat tccaggcgtc aatatcggag tcgtcggtca tgtggatcat
     1321 ggaaagacca cgcttgtcta tgggctcacg aaagcgtgga cggatcggca cagcgaggag
     1381 ataaaacggg gcatctcgat acgcctggga tacgcggatg cgacatttta ccggtgtgaa
     1441 gggtgcgccg gttccgattc attttcgacg acacccgaat gtccgatctg cgggacaaag
     1501 ggtgtcccgt tccgatccgt atcatttgtc gatgcacctg gtcatgaaac cttaatggca
     1561 acgatgctgt caggttctgc ccttatggat ggggctatgc ttgtgatagc ggcaaatgaa
     1621 ccctgcccac aacctcagac aaaagaacat ctgatggcgc tcgaacttgt cgggatcaaa
     1681 agaatagtga tcgtgcagaa caagattgac gtcatatcac agaaagatgc tctcaagcac
     1741 tacgagcaga tcaaggcatt cgtgaaaggt accgttgctg aaggcgctcc gatcatcccg
     1801 atctctgcac agaaatcgat caatatcggg gcagtgatcg aggcgctgga tgcggtaatt
     1861 ccggagatag agcgtgatcc gggcgcaaac cctgtcatgc tgattgcacg ctccttcgat
     1921 attaataaac ctggatcgaa ctggcgtgat gtaaaaggag gcgtgatagg gggatccctg
     1981 atccgtgggg ttttcaagga gggggatgac atagagatcc gacccggccg gcagatccag
     2041 gcagagaaca agatacggtg ggagccgatc acgacaaaga taacgactat caatgccgga
     2101 tccaaaaaag tcccggaagc aggcccgggt gggctgctgg gaataggaac caaactcgat
     2161 ccagccctca caaaaagtga cgcgcttgcc ggacaggtca cgggtcacac gggtgcgttg
     2221 ccaccggtct gggatcgcct ccgattccag gtcaccctca tggagcgtgt cgtgggtgct
     2281 atgagcgagc tctcgatcga accgctcaag caccacgagc ctctgatgct ctcagttggt
     2341 accgcggtca ccgtaggggt ggttaccagc gccagaaaag acctggcaga agtaaccctg
     2401 aagcgcccgg tctgcgctga accaggggca cggatcgcaa tcagccgcca ggttggagcc
     2461 cgatggcgtc ttatcgggat gggcgtcctg accgagtgac cgtcctgatc gatgcaaatg
     2521 cgctgatggc gcaactgcag ttcaggatcg atatctttgc aggactgacg gaatgtatcg
     2581 gtgcgtttga accagtgctt ctcgactgtg ttcgggatga gttgcgcggg cttgccgcat
     2641 cacatggaaa ggaaggatca tctgcgcggt ctgcgcttat ccttgcagag cggtgcacga
     2701 tcgctgagac cggaatgtat ggagggtcag tagatgagaa gatcctttat tatgcaagca
     2761 cgcatggatg catggtattc acaaacgacc gcgatctccg gacaaccctg cttgctcagg
     2821 ggattccggt gatctcgctc gcaggaaaac aaaggctgga agtatacagg aactaggtgg
     2881 atcatgtatt accggataaa actggcagac aaggtgcggg ttccacccca ccgtctcggt
     2941 gaggagctcc ggaaggtgat cctcgatgtg ctgcaggagc agcttgaagg gagcatcgac
     3001 aaggagattg gcatttttat tgccattaca aaagtgctgg atgttggaga aggcgagatc
     3061 gttcctggcg atggagcggt ctactatgat gtgaattttg agtcactggt cctgcgtctc
     3121 agtctccagg aggtcattga aggcatggtg gttgagacga ccagtttcgg tgcgtttatc
     3181 agccttggtc cgatcgatgc catgctccac gtgagccaga tctctgacga gtacatcaat
     3241 tatgatgaga agaatgcccg gctgatctgc caggagtcca aaaggtacat cgccgtggga
     3301 gagaccctcc gggtccgggt ggtgaccctc tctctgaatg aacgcgaacc acgcgacagc
     3361 aagattggac tgaccatgag gcagtccggc cttggaactg caaaatggct cgatgaggaa
     3421 ctggctaagg agaaagagaa ggagaaagag aagggtggtg agcgtggcgg gcgctaggaa
     3481 aaaactggtt catgtctgca gggactgcca ccgggtcgtc gagggggaga gctgtgcggt
     3541 ctgcggcagt gcgaaccttt cgagtgattg gaccgggtac cttgtcatca ttgatccccg
     3601 gcattcagaa gtggcaaaaa agatgaatat ctctcttccg ggaagatacg ccctgaaggt
     3661 ccgctgaaga tgctcctgct gcccgagagc cggcggtatc tgttcaggca gccgtttgga
     3721 gtactttgcc cggatatgaa ctgtgtcctc cccctgatcg aggggaaggt cgtgtatacc
     3781 gtaggggatg tggtcactca tcacctgatc cagcagggga tcgttccggc catcgcgatt
     3841 atcgatggtc atacaaggag ggaaccctgc aaccggtctc ctgctgtttt caggaagcgg
     3901 tgcggggcca ggaatcctgc aggaacgctc accattgagc ttcgaaacac ccttgacgca
     3961 gcagttggcg atccggatac agaggtgctc atcatggtcg atggtgagga ggatctggca
     4021 gttatccccc ttatcctctc agcacccgag ggggttgtgg ttctctatgg tcagccagga
     4081 gaaggagtgg tggtctgcga agtgaatgaa gcggcaaagg agaaagcaat tgcccttctt
     4141 tcgtgctttg tggacgatgg aaaagatctg ctcccaggct agtatcgcct ggtggctagg
     4201 gtataaataa tccgaacacg aattatatga ggatacgatg gatttcgagg ttaccaggga
     4261 taccaggaac gagctcctgg caaggaggga ggtggacttc aggctccggt ttgaaggtcc
     4321 caccccgtca cgaatgcagg tcatcggcaa gctggccgcg ggtctgaatg tgagtgagaa
     4381 gctgctggtg cttgacacca tgaagaccag cttcggaaaa accgagctca ccgggatggc
     4441 ccgggtgtac gatacggaag aacagaaatc aaagaccgag agggcttacc tctccacccg
     4501 cggcatgccc aaaccgaaag aagagggagc ctgatggcgg caaagaaagg caaggccccc
     4561 tcagtgcgcc gggcctcctg ttacaagaca gaaggtggta aggcgatatt ggcaaagaag
     4621 ttctgcccgc gatgcggacc cggtgtggtc atggccgatc acaaggatcg tatggcgtgc
     4681 gggaagtgcg gatacaccga attcaaaaaa taaccgctga tgcctgtttt tgggcggatt
     4741 ctcggaatcg agggaacagc ctggaacctc agcgccgctg tttttgataa cgatctgatt
     4801 acgcttcatt cgaaaccata ccgtccgccg actggaggaa ttcatccgag agaggctgcc
     4861 cagcatcatg catcagagat gaaggcggtt atctcgaagg tgctgtctga acctgatggc
     4921 atcaccggag tcgccttttc acagggtcca ggtcttggcc cctgtcttcg aaccgtggcg
     4981 actgctgcca ggtcccttgc cctcggcctc ggtgtgcccc tcgtaggggt aaatcattgt
     5041 gtggcacaca tcgagatagg caggtatgca acaggatgca gtgatccggt tgttctgtat
     5101 gccagtggcg ccaataccca ggtgatcggg tatctcaaca aacgataccg gatatttggc
     5161 gagactctcg atatcggcat tgggaatgcg atcgacaagt ttgcccggag caagggactt
     5221 ccccaccccg gaggccctgc cattgaggaa cttgcacgaa aggggaggta tgtggacctt
     5281 ccgtatacgg tcaagggaat ggaccttgca ttctcgggtc ttgtgagcgc tgcgcgggag
     5341 tgcagggcac ccatagagga cgtctgttac agccttcagg agaccgcatt tgccatgtgt
     5401 tgtgaggtga cagagcgtgc gctcgcccac accgggaagg aagaggtgtt gcttgtcggg
     5461 ggggttggtg caaacagccg attcgaggag atgctctccc agatgtgtgc agacaggggt
     5521 gcgaatctgt ttgtgccgga acgcaaatac ctcggcgata atggtgccat gattgcgtat
     5581 accggcaaga ttatgctgga gagcggatat tctgttcccc ccgaagaatc atacgtgaat
     5641 ccctccttcc gttccgacca ggtggaggtc acctggcggc atgatgatcc aggcgaacgg
     5701 atcagggata agccactcga tcagattacc cgggcaaccg gtgcggaagc agaggtcctg
     5761 ctcggagaat tattcgtgga gaaacggaga gtccggaagt tataccgtca gcaggagctg
     5821 aatgatcgac tgatcgctga acggacacgg gctgaagcac gccttatcgc ctcttcccgg
     5881 aaggaagggg tacccacccc ggtcatacag gacatcaccg gcacaacaat ccggatggag
     5941 aggatctacg gaagccttct gaaggattct ctctccccgg tgcatctgga ggaggcaggg
     6001 aggaccgtag gaacgcttca cacggcagga atcgtccatg gtgatcttac cacgagcaat
     6061 ttgataatgc gggaggggag atgtgtgctg atcgactttg gtctttctct ggcatcatca
     6121 gagatcgaag ccaggggggt ggatctccat gttctcttcc agacactcga gagcaccact
     6181 gataattttt ctgaactgaa agaagcgttc acacgaggct atgagcacac cttttctgga
     6241 gccgatgagg tgctcgcgag agaacgggag atagagaaga gagggaggta cctgtgaagg
     6301 tcgcagtggt gacctccaat cgccacaagg cggcagagat cgctgattat tttgggacac
     6361 ttgtagaggt catgcatgtc actatggaaa tccccgagta ccgccacgac gatgtggggg
     6421 agattgcgta taaaaaggcc gaatatgcat atcatgctct ccgttgtcct ctccttgtcg
     6481 atgacacggc tttctcaatc gaagccctca agggcttccc cggaccgtat gccgcatatg
     6541 tcctctctac ccttggaaac cacgggattc tcaagcttct tgagggggag aataacagaa
     6601 atgcatattt cgagacggct atagcgtttg caaccaccca gggaacaatc cacgtgttca
     6661 ggggaaggat cgatggaacc attgtctctc cccgtggtga ttcgggtttt ggatacgatc
     6721 cgatttttga ggtggaagga aaaaccctgg cagagctgga tcgttcttcg aagaaccaga
     6781 tttcccatcg tgcgcgagcg ctgtccttgc tgaaagcctg gatccttgca ggggatcccg
     6841 ggatgccatg acaaaaccgt taagaggaag catgacgttt aataatagtc caagaagtgg
     6901 tgtccctcat ggcaagattt cctgaagccg aagcaagact cctgaatgtc aagatatgca
     6961 tgcgctgtaa tgcccggaat gcgatccgag caaccagctg tcgcaagtgc ggatacaaga
     7021 accttcgtcc caagaataaa gagaagaagg cctgatcagt caggcactgt aaaaggtgac
     7081 cctttttcct ttctggctgt attctccttt gaacgtctcg atattccttt tataaccgat
     7141 gcggtcggcg aacccaagct cacgcaagcg ttcccgtacc cgcataactt cctgttcatc
     7201 cctccagtca ggggtgtata tgtaaattac tttcatgctg tcccgtgaat cgggatttgg
     7261 ttttgcagtg cttacctttg ctgagattcc gagttcattc cggcaggtcg catcgcggac
     7321 tagtatccag gcctcgtcaa cacgttcggg aggaaggaag atgagccatt tccccgcaag
     7381 ttcatcctca atcgcgtccg gcttcacatc aggagcatcc tgcacaatcc agtacatcct
     7441 ggtggtccgg cttgggatca ccccctcccc ctcacgaatt gcatggtaga tgacatgggg
     7501 ggacctgaaa cggaagatga gtgcctcgcc caactgggga tagtcatcgc agaacttcct
     7561 gaatatttcg aggaattgtg cttcgaaatc gattcctttc tccaccagct cgaagagata
     7621 cggtcccctc tcgcgaagcc cggtattcag catcacctcg aaaagaccat aagccacgtc
     7681 ggccagtgct tcgggatcga ctttttccat atgttaccgt tatacggagg cactaaaaaa
     7741 tacttaggtc ccaatgtcat ctggtaccga gctcccgtag taccagagat gccgcatgct
     7801 ctcctgacag gagcatcccc ccgaagacag gacccatccg ggtttcaccg aagaccgcat
     7861 ttgctgccat tccggccacg ataagccccg ggaatatctc tttggtatgg ctgattatca
     7921 tggattcagc cttttcagcc cacatgaagc cctccccctt cacaaaaacc tggtccgttt
     7981 ttttccccac gatacgtgcc acgcttgcat catggccagt agcatcgatg gtatagcggc
     8041 accagagcgt gagcgggtcg acatgaagtc cggctattcc caccggtgtc cagttcacca
     8101 caaggcctga gagcctgttc tcgctcctga tcatgacgtc ctcaaccgtc atgagggtaa
     8161 gaaattcaac ccctgcgtcg catgcagcag aggtaagttt cgagactgct tcaactgccc
     8221 cggcaacata gtacccttca gtataggaat gatagtgtat cctgaaatga tccaggaggc
     8281 gccgtgcgct ttcctgaacc acgatccggg gaaacatcat acctcctccc cacatccccc
     8341 ctcctaccga taattttttc tcgatgaggg taaccgccag ccctgcctct ccaaggaagg
     8401 aggcagcagc aagaccggag ggtccaccac ctacgattgc cacatcggtt tcaatgcaat
     8461 ccagcagggc tgtcagctca gactcaataa tcgctctggt gataatccgc tcgtcaagct
     8521 ccatctcttt cctcaatcca ttcgtattcg cgatagataa taagaaatta cttcaaccgg
     8581 atcggaaaga agcaggatga aaacccacgc tttctctctg gtcggcactc tcttttgaca
     8641 gattctcgta catgaacaat tggcgcttat tgtgaaaaaa caccaggtac aggcgtattt
     8701 tcacattccg ggaaggattt cctcttcact tgggagaatg tttatgaaca acgcattcag
     8761 aacttcttat catgaaacca tggaagcgtg actgggggct gacaggcagg atatggctta
     8821 caatgttcct gctgttcctg gtttaccttg cctttatcgc agtactctcc atgcttggta
     8881 tcggtctccc gttcctggta gtcatcgtat ttctgatggc gtttctccag tactttttct
     8941 cggaccgcct cgtgctttgg actacgcgct cacgggttat agaacaggag gagtaccctg
     9001 agctccaccg cattgttgag agatgcgctt ctgaagcagg cattcctaaa ccccgaatcg
     9061 ccatcatgca gaccccggta cccaatgcat ttgctaccgg gagaagcccg aaacatgcag
     9121 tggtcgcagt aaccgattca atcatgcgac tcctcactcc gcgggagctc gaagcagtga
     9181 tagctcacga aatctcgcac atcagaaaca gggacgtgct cacccttact attgctagtt
     9241 ttgttgccat gattgcagct ctcgtgatga acaatttttt gtttgctgcc atgtttggtc
     9301 ggagagattc cggaaatgcg tggatcattg ccgggattgt ggcagtcgtg gtctatttcg
     9361 ttgcacagct cctgattatg gcgctttcac gctaccggga atttgcagca gacaggggga
     9421 gtgcctatat caccgggtct ccacgggatc tcatatcagc ccttggaaag atcagcggga
     9481 ggatggaggc agtacctcct cagcagaagc aggcagtgga gaccgcaaac gcgttcttca
     9541 tcatccctgc gctttcgggt tccaccctgc tcgaactctt ctcgactcac ccagcccttg
     9601 aaaagagaat cgctgcactt gagcaggttg agctccaact caaggggcgc gcataatccc
     9661 tttttctcgt cacccctttt ttttgtggtt tccggacgaa aaacccggga taagcatata
     9721 acaaaaggaa taagtggaag tatctcaaaa tagaatggcg caattgcatc gatggtctag
     9781 tggcatgact taggccttcc aagcctatag cccgggttca attcccggtc gatgcatgcg
     9841 ggctcgtggt ctagctggtt atgacgtcgc cttcacacgg cggaggtcag gagttcgaat
     9901 ctcctcgagc ccattcagga atattctctc aatttacgtt catggagggc gaggaaatta
     9961 tcatttttat cgttccccgc ccctattgtc aggctggtct tcctcgaatg tgaatccttc
    10021 ctcgaatgtg aataactcta caacacgtat gatcgggtca tcatcggtca tctgacaatt
    10081 cgtttagtgt tctcttcatc gtgattccta tcagaatcac attaaaaatg cctcccttga
    10141 aaaagaatga aaaatgcatt ggttgttccg aaattttgac aactcgtcat ggtttcagaa
    10201 tcaatccttt taaataggac gattttcaat acctgatcat aaggagctga atgagttatg
    10261 actccggaac agtacagagc gattttatca aaagcaatcg aacgggaagt ggaatcttac
    10321 acctattacc ggaccatctc cgacaaggtg aaggacgcct acctgaaaaa gatctttgct
    10381 gacctcgccg gtgatgagac caagcaccgg gaattcctgc aggatctcct cgcaaaggaa
    10441 ccgaaaaaac tgaaaatttc agcatcgaag gactataaga tcgccgattc agtgaaggca
    10501 cccaccctct ctgctgattt aaagcccctt gatgggatcg tactcgctat caagaaagaa
    10561 cttgaagcaa tgcagatgta cacccagctc gctgatgccc agactgatcc agaacaaaag
    10621 aatctcttca cagacctcgc agcaatggag cgggggcaca aagcccggct tgaggacatt
    10681 tacacgaaca tggcatttcc cgaatcctgg taagggaaat atcttttttt ctatttcgct
    10741 ctcttcacta attgtcatta attttgaggt ctcccgcaaa aaaaaacctg gatatttacc
    10801 ctgtaggtga caatccatga aagcgctttt cggggttttc ttgataattc ccataggtaa
    10861 ttgcagaatg agggtcctgt gaatgcatat actccttctg cagctgtgaa agccagagtg
    10921 gtatcttaca ccttcgctac tctaataatc ccacagacaa agatcatacc agcatatatg
    10981 cataatattg ccaggagttc acgggtgaat gcggttcttg cactagcagc aatcagcata
    11041 ctggtagcat ttctgatccc cgtgcaggtg atgggggatt caccatctga aattgcttca
    11101 accttcatcc agaaaggaga tgatctcacc cttcggaaag agtaccacga cgctctggaa
    11161 gcctatgaat cggcggttgc aagtgatcca tacaacagca atgcctggaa caagcttggg
    11221 gtagcccata tgaacaccgg gagataccag gatgcggttg aggcatttga gcgatcactt
    11281 gccatcgatc cctataatca gaaagcatgg aataataagg gggatgccct tgcagcactt
    11341 ggggaatatg aggatgcaat ccttgcctat gaacaggctc ttcgcctgaa ttcaaatgat
    11401 cactatgccc tgctccgaaa agggatcagt ctccagcaag ccggcttttc ggctgaggct
    11461 atgcttgtct atcaggaagt tgtcagcctt gctgaaaagg aagtacgaaa acaccctgat
    11521 tacgcacgtt tcgatgggga attgtggacc aacaaagggg aagccttcta caggcttgga
    11581 cggtatgagg aagcattaga agcatatacc accgcgttaa ccatcaatcc aaaatatgat
    11641 cgcgccattc agggaaagat acatgcagag gaagagatcc tgcgggcaag gggtgcaccc
    11701 cccactccga gtcctgttat cgaagaaaaa ggagtagata ttgggctgac caacctctcc
    11761 atgtcattac ccgttcttct gggagccctt gtcattgcgc tatttttcct ccgtctgagg
    11821 ccgccctgcc attgatacac ggggaacgaa tttttacatg ttctatcatc ttatcctgac
    11881 cgataagtgc aatctttgct gccgttactg ccgtggaaat gaatccgagg tgtttctccc
    11941 gcttgaggga gactccggca tcgatttgga tcttccgtta gaactggcat acgacctcac
    12001 cgacctctat tcttttctgg agagagatcc atcgccgtgt atcacttttt acggcggtga
    12061 acctttgatc agggctgacc tcatcaggga gatcatggac gacgcaccgc acgtctcccg
    12121 gttcatgatc cagaccaacg gccttctcct tgatactgtt gggcaggagt atctcaatcg
    12181 aatggagact attctggtat caattgatgg agctgagacg ctcaccgatt attaccgggg
    12241 aagcgggaca tatcgcagga tcatctcaca agtcaacaca ctcctctcgg aaggatatca
    12301 gggggaactg atagcacgga tgacagtgtc ccggaagact gacatccgtt ctgcagtaca
    12361 tcatctggca ttcaaccatg aatgtccttt ttcatccatc cattggcaga tcgatgccga
    12421 tttttccgga aatcccggga tatttgactt cctgggatgg cttgagcagt catataaacc
    12481 tgggatcaga cgcctggccg gcgattgggt gaaaataatg gaggaggaag gccgggtaac
    12541 tcggtggtac ccttttctcc agaccaccga ggatctgctc accggaagaa catcccggct
    12601 ccgctgtgga tcgggctatg cgaactatac catcatgacc gatggtcata taggtccctg
    12661 tcccatcatg accggcatgc gtgattatta tgtagggcat ataaggaccg ctgacccctg
    12721 ctcccttccg gaggtcggaa tacgcgaacc ctgtccggac tgtgagatct atggtttttg
    12781 tggggggaga tgcctctatg caagtgttgt ccgaccctgg tcggcagaac agaaagaact
    12841 tatctgcagt gcagtgaagg ctctcttctg cgatatgtcg gatatccttc cgcggatcag
    12901 gacatgtatc gaatcagggc ggatccagat ggcagatttt tcccacaccc gctttaacgg
    12961 gtgtgaaatt attccctgag gtatccgctg ctatttgtga tagggaatcc ctctcctgat
    13021 gcattcagcg cgatagatct gttcaatgag catgagccgg accagctcat gaggcagtgt
    13081 catccgggaa agggagagaa caagatctgc attgctgatc acttctggag acagcccgta
    13141 tgttccaccg ataaaaaacg agattttccc tcgccctttc aattcccatt ccgccaacaa
    13201 gtccgacagt tctctgcttg agagcatcgt tcctcccgga tcaagtgcaa caagtatgtg
    13261 agtatctggt gcagcggcca gcagcttcct cccctctctc tctttcgcca accgcttttc
    13321 tgtatcagag ctgttcgaag gtattcgttc ttccgggaga tcggtaaagg ttattttgtt
    13381 cgcggaacag agacgtgtct cgtagacgcc gatcccctcc ctgatgaacg actccctgat
    13441 ctttcctatt gcaacaatct gtatctgcat ggtttttcct atccattgtt gtgcatcaag
    13501 gcagagccac ggagcagttc atcgcacttt cacagtgcac ggctgcatta tttgaatccc
    13561 cttgctgatg aactctatgg agaggtaatg ggaaaagccc agtcaaggag tgtgggcaac
    13621 cccctccccc tctgcagtcc taaagaccct gaagagaaat ccccggggct cccccttttc
    13681 tccgggcaca ctctcttcga atgcttcgaa ttccggtgaa ttcttttggg aatttattat
    13741 cagatccttc cccttggggg ttaaatgatg ctgaattatt acgatggtat tgcattattt
    13801 tcatgaatga agtaaaaatt ctaaatatcc ttcgaacgag tcctgcaaaa atgggagggc
    13861 aattgcgacc tgtgaaaggg gtgggtgagc gataaccggg tatcaggtca caagttcaga
    13921 acgcacggtg aagaagacgg gtccccggag atccactttt ttcttctggt ccgtgatata
    13981 cctcatggca taatcttcaa tcctgatgtt gatatcgctc ggcagctttg ccatttttac
    14041 ctggactgtg acaggccgac cgcaacgaac gccctcttca attcgtgccg cggcaagagc
    14101 agccgttgca gccaggtagg taatcccgga atccaccccc gcctcgcgga cttccttcca
    14161 tttctcagtg gcaggtactc ccatcactga gccggaaaga acatagatcc tgttcatgca
    14221 cgctggtccg agcagtcttg tgccgctttc cggctcctcc acgtatacac gaacaggtct
    14281 cccttcgatc tcactttccg atgccagaaa cgaacagggg cccggttctg ttccatgaga
    14341 ttcagcagct gatatgatgg aacgggcgag gttttttccg atagggcttt tcggttcttc
    14401 cctgaatgat attgcacgcg cgatctcgaa atcggtcaga tcgatgggga agaactgggg
    14461 gaatgtgagt ctccttacgt catctgactg gtaggcaatc attgcgagcc gctcaacccc
    14521 caggccgaga ttcatcaccg ggatacctat accgtattca cccagtgccg aaggcgaata
    14581 catcccgaaa gtagccacct ctacccagtc atggaccgga tggcgggcat acacctcggt
    14641 ctgggaacct ggcatataat atttcgatcg tttttcatca ggctggaacc ggaaatcggt
    14701 gtaaccaaat gaagagagga gcgctctgct cactgctttc ccatcctcga tcgtcacgtc
    14761 atcccctgcg atgacacacg atgcagaatg gtagctcatc agcctggtcg gaccctcttc
    14821 ctgctcccgt cgaaaacagc gatctattga gaacatccgg agaggaagcg ggcgcttctc
    14881 ccagatatgc gagagcgtca tgaaccagcc gcttgtcata tggctccgga gggtatttcg
    14941 ggaggaccga ggctccagtg atctgaatgc ggagaacact ttctcataga tctctacggt
    15001 tgctccatca tcgattccaa gcgcatggga tgtggcatga ataagatcat cgccgtcgat
    15061 aacagatttt ttatatgact gaagaagctc gcggagacga tcttcctcct ccgcgctcac
    15121 ctgctttccc gttatggcag caattccgtc gagttctgat ttccctatac cctggtttgg
    15181 ccgctcgagt cctcctaggt aaaatactcg gtcgagcact gcactggctt ccgggccaaa
    15241 ctggcggtac acttcctgct cctctacgat gataggattg caggcctcct caaatcccag
    15301 aaggagatag gtctccctga gacggcccac catctcaaag atagggtggg gcaacgcctt
    15361 ccgatactgc agtcgtggat actcctgctc tcggcctgga ggagtaacga caagaggacc
    15421 cttgtgccag gcctgttcga aatgatccct cgcagctttc cggtattctt ccgggttgaa
    15481 tctcatgctg tgttctccag aaggatgacc ctgctcgggg cattctcatt ggtgatccga
    15541 tagtcacagt attccgcaat cctctcagca aactctttta tgtaggcatg gtccggcata
    15601 tgctccctgt tcaatcgttt tctactgtat ccaagataca tatatccctt tatttcgaca
    15661 tatcgggcgt ttgacttctc aaacagtgcg gcatactttt ccggactgtg atcgttgaga
    15721 ccccggacca gggtcacccg caccgccgat ctcctagtcg agagatgggt caggctcctg
    15781 catatccggt cccagtagtc tgctgaaggc ctgcatatgc tgagataggt ctcttggtct
    15841 ggtgcaggaa gggagatgta ggtctggaag ggaaaacatt gtgaaatcac atccggattt
    15901 gttccgttac tcaccagaaa cgtggtaaat ccttgtgtct tgagcagatc gatcagttca
    15961 gggaggtgtg aatagagtgt gggttcccct gaaagggaga tggcaatatg tgccggcctg
    16021 agggcttctg caaatcgctc actggttaca tagggcgaga ccttgtatcc tgaaagggct
    16081 cgtttctgga gatcatagag gcccgcgagg atctctccag gggtgcattc cctttcgccc
    16141 ggatactcat gttcaaatga tcgccagcag aaaagacacc ggtgattgca tcggagggtg
    16201 ggggtcatct ggatgcatcg atggctctgt attccgtaaa actggtgctt gtaacattgt
    16261 gctcctcctt tcagcgcctt tttacaccag agacagggct tgacggctgc agatgattct
    16321 ggtgagaaga actgataccc ctgtccccgg agtgccttac atggtgacga gggcatcgat
    16381 accaagtgtc ccgagtgttt ccgcgagcgt gtttttcgtt gctttcacca gcgtgagtct
    16441 gctctgcctg gtaactccct tgctcttcag cactggttca aactggtaga agacattgaa
    16501 gaagtctgca agttcccttg cgtaagtggc aacgagatgt ggttttaggt cctgtactgc
    16561 attcctgatc acaaaaggga attttgcgat atgcttggca agcgcgatct cgctctccat
    16621 ggtgaactca aatgccgtgt cgaatccacc tgctttatcg agaatgctgc aagcccgtgc
    16681 atgggcatac tggatatatg gaccactctg tcgctcaaaa tcgagggcct ctttccagtt
    16741 gaacacggtg ctcttctcgg gggatatttt cacgatgtcg taacggattg cagcgatggc
    16801 aaccgagcgg gcaatctcct gtttggtgtt ctcgtcaaga tcatccctgc gtaacgtgac
    16861 ttcctcataa gcccttcgtg tcacctcttc gataagttca tctgcagaca cgaacttccc
    16921 cgcccgggtg ctcatggatc cttccgggag cgagacaaat tcaaagtgca cgatctcagg
    16981 tgctttctcc ccgattagtt tgagcgtcgc gatgagctgt gcccctatca gcttgtgatc
    17041 agccccgaga acatcgatta aacggtcgaa gtttcttccc ttccaaatat ggtatgcgag
    17101 atcccgtgca gcgtagaccg aggtaccgtc acttcgccgg aggatatact ccttttcgaa
    17161 accttcgttc gaaagatcga gccacagttt tccgtcttca tgacactcct ccatccttct
    17221 caccctattg agaaggcgtt ccgtatcccc gttcctgaca aagtcgctct cccagacaaa
    17281 ccgatcatgc cgggcagaga ggagcgccag ggtaaccttg aacccttcga gacattcgga
    17341 gactgcattc ctgaacaata ccgctgtctt gggatccccc ttctcaacct gctgcatcag
    17401 gaggtcgatc tcaggtacga tccctggatc tctctctatt tcacgatttg ccgctatata
    17461 aacgcgagcg acatgatggt cccctttctc cccatccttt cgtgctccat caaggtgtga
    17521 aaatccccat gcgacaatgg caatctgccg gcccatgtca ttcacgtaat actggacctc
    17581 gacccggtag ccggctttcc ggaacagccg tgcgagggta tctccaataa tagtattcct
    17641 gatatgaccg acatggagag gcccgttcgg attggcactg gtatgttcaa gcacaacccg
    17701 gcctcctttc atcaaaagcg tgccatagcc aggctggagt gcggcttcaa ccgatgactt
    17761 gagaaacgtc gggcccgtct gaaagttgat atagggtcct ttggtctcga tggtgatccc
    17821 ttcaagggac gaatccgccc ggagtgctgt tgcgatctcc tctgctatct ggacgggcgg
    17881 acggtggagt gccttggccc tttcaaatga gatggccgtg gcaaaatccg catggatccc
    17941 gccatcggtc agggtggggt gaggtattcc ggttgctgcg gcaagagccc gctcaattgt
    18001 cccatagatt tcaagataca ttatgatccc gtcctgtacc gaagaattgc agcaattccg
    18061 ccaaacgcgt tgtacaggat tgccccttct tcaaaatcat cggagatgat ctccaccctg
    18121 gtgctggtct gatcagcgag attcgttagc tcttcaacaa tatcaacctc agattcgaca
    18181 atcagcggtg cggtgcataa ccggcacgaa tgggtgagaa tatcttcaac cgttttgcca
    18241 ggttcaaggg aaaccgtcct ctcttcagaa tggccacaac tctggcaggt gatcttgacc
    18301 cttgatttcc gaaggcggct tgagaggagc aggacatcga cagaaccgag ctcgaggttc
    18361 tgcctgacgc tctcttcacc gtatgaggca agcccggcat ctttcaccag ctcctttaag
    18421 aaacgctcca tcacctcctt ctcttttacg acttcctgtc ctttgagagc atcttttgca
    18481 gcatccacca gctcagacaa gccatcctcg tttgtgtacg ccacatcaaa gagtccgatg
    18541 atccgtttct gtatctcatg gtggaggtat tttcctgcct cgaactcttc ctttgtaggg
    18601 ctcggtccac cgatcagcac accctggaac cgctcgaaaa aatccttctc ggcgaggaaa
    18661 attgcactgg cccgctcgcc aatcttggta tagaattcat caatcgcaat ctcacggagt
    18721 cgctggaacc tggcactcga ctgacctcct ttcctctgct ttcccgggac ggttgaggtg
    18781 actcctccga tcggctcgat gcggttgccc cgtaagaatc cccagtatgc ctcacgcctg
    18841 tcgagaacaa ggagcccgta caccatcttc tcctctaaca tctgctttaa gggctcaagc
    18901 tcgaaattcg aactgcatcg gtatatgtaa aggttgaggg gttcaggcgg ttcgatgatc
    18961 gtgcattcga ggtcggagcg gtctcctccc atctgtatgg tgccgcagaa caccgccatc
    19021 ccattggagg gaggactctt gtaatatttc agacgtgaga ggattgagga gatggcactc
    19081 tggacgttgg tccgggtctg tttactcttg atgtttgcac attgcccgaa ttcgtccctg
    19141 agctgagcgg tgacatcata gatctgcttg tcgggaggga tatagagaga gataagctct
    19201 gttccactcc cttgttttgc ctgcagcttc tccagggttt tcttaaattc atatcgtcgc
    19261 cgcgcgttat cca
//
LOCUS       DAOOCO010000011        18423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2662_length_18423_cov_4.840973, whole genome shotgun sequence.
ACCESSION   DAOOCO010000011 DAOOCO010000000
VERSION     DAOOCO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 18423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18423
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_18423_cov_4.840973"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..610)
                     /locus_tag="PK154_01410"
     CDS             complement(68..610)
                     /locus_tag="PK154_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HPW09751.1"
                     /translation="MRELRIHGRGGQGSVTAAELIAVAAFEGGMHAQAFPAFGVERRG
                     APVQAFVRFDNKKIRLRSQIYEPDYIIVQDSTLIKDVNVFFGMKEGGIAIVNTEKAID
                     SPVPKGVKVITIDATSIALQKIGLPITNTALMGAFAAASGEIAFTALEDAVKRRFRGD
                     LATKNIAAAKAAFDAVKGAA"
     gene            complement(807..1679)
                     /locus_tag="PK154_01415"
     CDS             complement(807..1679)
                     /locus_tag="PK154_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HPW09752.1"
                     /translation="MFIGIDHGTTSIRFSTGESWFKITREEARSFCWQDLRTLCPVDE
                     ISGIALTYSMGDGISSITPIELVVDRGIRSRDGAGKHVGGGTRVFDQIRESGLPAVVI
                     PGLHRDSPTDTRFKVYSHQASPEKIGIAYRVCCDLGGDVVVSDVSSNTVSLLIKDGRI
                     AGGFDACIFAPGLEHGALDVDAIRSVDAGISTANEAFLRAGVNHTLPEQERLSAVALF
                     SAMECAALLVLNPGARIALAGSLGPRIAGEVSALLGREVAVYDEWCASSGLALIAREV
                     FQGGSEILGISVDR"
     gene            2019..2969
                     /locus_tag="PK154_01420"
     CDS             2019..2969
                     /locus_tag="PK154_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW09753.1"
                     /translation="MKAVVTGGAGFIGSHLVDALVERGESVTVIDNLSTGDIRNIAGH
                     IDSGAVRFIKHDLLLDGWQEHLRGAGRIYHIAADPDVRGSATHPEEVYTRNVHATVRV
                     LEAMRRAGCAEIVFTSTSTVYGEAALIPTPEEYPRMEPISIYGGTKLACEAMISAYAH
                     TFGWKSWVFRFANIIGERSNHGVIWDFIHKLRQNPRELEILGDGTQTKSYCSVPACVD
                     AVLFAVAHASAPFTCLNIGSEDWIDVTTIASIVADEMGLSDVSYHYTGGDRGWVGDIP
                     RMLLSIEKIRSLGWRYEVTSQQSVREAARALVLETGYLVR"
     gene            2975..4141
                     /locus_tag="PK154_01425"
     CDS             2975..4141
                     /locus_tag="PK154_01425"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPW09754.1"
                     /translation="MRERQKKYLVILGDGMADEPLAELGGKTPLEYARTPNMDRIACE
                     GTMGLARTIPEGFEAGSDIANLGILGYDPRSCYTGRGPLEAASMGLEMAPGDIAYRCN
                     LVTVVDGRMADFSAGHISSREGASLFASLSEHLPDISLSPGISYRNILVVHGGGGATT
                     CPPHDIVGELIEPSLPSGKDAGILRECMEKSREVFADHPVNRARISAQKSPATQIWPW
                     SGGKKPRLIPFRTLFGREAGIISAVDLLQGIARLAGMEVIRVPGATGYLDTDYQAKAS
                     SALEALERLDFVYVHVEAPDEAGHLGNVWEKVKAIERIDELIGTVMEGFSGVIAVLPD
                     HPTPIRIKTHTSDPVPFAIWGKGRDATTSFSEKEGSRGGYGVIDAPRFLPLLFS"
     gene            4157..4672
                     /locus_tag="PK154_01430"
     CDS             4157..4672
                     /locus_tag="PK154_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09755.1"
                     /translation="MINHIRAGGEYDLMGGHKTLTIGVTVNLEHYENLRVEVSGDADS
                     PGDAEELTRFLTAILGSFGRNDPATAERIDSYRRRVLPGSASSVSAGGEGAAILPPAA
                     ESGGEKQTESEPAESRKAQASAPVPPAGAAASVSGPTCDSCGTPVSPAEQKMSQLFTS
                     RILCRACMKNL"
     gene            4701..5825
                     /locus_tag="PK154_01435"
     CDS             4701..5825
                     /locus_tag="PK154_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HPW09756.1"
                     /translation="MKKNLLMWDETLFRDPEVFEIDYVPEQFNHRESQIRELAFQIHP
                     GLRGGRPLNTICRGLPGTGKTTSVRKIFSEIEENTKKLVPIYINCQIDNTKFAIFSQI
                     YRRLSGHLPPASGTSFKQVFDAIARMLLKEEVVLLVALDDANYLLYENEMNRVLYSLL
                     RSHEAYPGTRIGVIVIISDMDVDLSQAVDARVSSVFRPSEVYFPPYSEDEVREIVRER
                     VLQGLYPGCLPEEMFSLVVEQTQKSGDLRVGIDLLKRATLNAERRASRVIERDDICQA
                     YDVSKYLHLSFSVKSLKDEEKEVLRALANHSTGDSEMNAGEVFRAVKETVPIGYTRYY
                     EIIRKFDAMRLINLHYRDGKGRTRVISLRYDPAKIINYLG"
     gene            5903..6850
                     /gene="mch"
                     /locus_tag="PK154_01440"
     CDS             5903..6850
                     /gene="mch"
                     /locus_tag="PK154_01440"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HPW09757.1"
                     /translation="MLSVNELALEIFENLAEYAEEFNAAYHELDNGARIVDCGVSTRG
                     GYAAGRAFTEICMGGLGEVNFRMGHIKEFPVPFIDVFTDYPAISCLGAQKAGWTIKQN
                     NYFAMGSGPARALSLKPKHTYEVIEYEDDFDSAIIALESDHLPNAGVMEKIAEECQVD
                     VANLCAVVAPTASLVGSIQVAGRCVETAVYKLNELGFDTKKIVAGFGTAPVPPVKKDA
                     TRAMGTTNDATIYHGRIMLTMKAPEIKEYLEKIPSNKSKGYGKPFYEIFKEAQFDFYK
                     IDTSLFSPAEVVINELTEGVVYHCGAVNPDVTLKSFGLI"
     gene            6870..7502
                     /locus_tag="PK154_01445"
     CDS             6870..7502
                     /locus_tag="PK154_01445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW09758.1"
                     /translation="MAEHGPVSGQAAWEAGYRKSGRRYGRAPPDLPPFPPGTRILEAG
                     CGDGKSLLAMTGRGWDIVAIDFSREAIRISSRQNMLERGRFVQADAAALPFTDGSFDV
                     VSISHLLGHLPEEHRSRAASETERILVHGGFAYIRVFSTRDFREGKGILTEPHTYLRG
                     DGIMTHYFSEKEVETLFPTLVRMELSTSDWSMRIKGEHMNRSEIIALFRK"
     gene            7731..7804
                     /locus_tag="PK154_01450"
     tRNA            7731..7804
                     /locus_tag="PK154_01450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7765..7767,aa:Val,seq:tac)
     gene            complement(8548..8919)
                     /locus_tag="PK154_01455"
     CDS             complement(8548..8919)
                     /locus_tag="PK154_01455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW09759.1"
                     /translation="MGSGQVISGFDEAVIGMTPGTTKIVTIPPEKGYGLYNKSLVFRV
                     DQAEVNEFLQDQESMGNLLGFQYPGIGSGYRWINSDGSPGYMRFFNATDTMVTVDLNN
                     PLAGQDMVFEITLVEIVHPGT"
     gene            complement(8888..9139)
                     /locus_tag="PK154_01460"
     CDS             complement(8888..9139)
                     /locus_tag="PK154_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09760.1"
                     /translation="MKTNPLTIGSRDSVVWLTAALLICYAFLLVAGCSAPVQEGVKTN
                     DTVKVRYTASFQDGTIFDTNVNSTPPGIHRGKWAGHFRV"
     gene            9310..10203
                     /gene="nifB"
                     /locus_tag="PK154_01465"
     CDS             9310..10203
                     /gene="nifB"
                     /locus_tag="PK154_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HPW09761.1"
                     /translation="MTAEGYREAEVAGRKVKWDPEQLRRIKEHPCFSEQACHAFGRCH
                     LPVAPRCNIQCNYCIRDFDCVNESRPGVTTKVLDPDAAIDMVRKVMEKFNYIKVIGIA
                     GPGEPLANEGTFETLRRLHKEFPSVIKCISTNGLLLPDKIDLLHEYDVGNITVTLNAI
                     DPEIGAKIYQFIDYKGKRYTGVEGAKILLENQLKGIEMAVDRHMIVKVNTVYIPGINE
                     DHIPEIAKKVGEMGVYNFNLIPLIAQYKFADITPPTPEMKRTMQDECSKYVKQMRHCQ
                     RCRADAIGRLGHDVQSCMYSE"
     gene            complement(10347..10682)
                     /locus_tag="PK154_01470"
     CDS             complement(10347..10682)
                     /locus_tag="PK154_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09762.1"
                     /translation="MDYSRTGGIAAFDDRLVIFDNGQAVYSRRIAKGEFTLPQDRLSE
                     MKSLLSDADFPSLASSYPAPSPGADYFSYTITHDGKTVTTETGGIPDPLIAVISRLDA
                     ILADYAPLS"
     gene            complement(10927..11253)
                     /locus_tag="PK154_01475"
     CDS             complement(10927..11253)
                     /locus_tag="PK154_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09763.1"
                     /translation="MELVDTLRVFLENGDDWERKLTSIRGVTILKLPQTKSRPASIAI
                     EINPLTDKGTPMKKKGVMIMGQAELNAFREIFNNEKVGVLLSSLESLVPARKGAKGEE
                     GDVLQI"
     gene            11421..12299
                     /locus_tag="PK154_01480"
     CDS             11421..12299
                     /locus_tag="PK154_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HPW09764.1"
                     /translation="MPRITLGEHCPFSLDLTLGCGQVFRWQKSGEWWTGVVEKDLIRI
                     RQKGRTLIWEGSTQERIRDYFGLDHDLGRIIESVDRDPVIHLAVANSTGLRLIHQPPW
                     ECLASYICATYANIPGIRRKIALICDTFGDEISSRKDFRLSTFPSADVLAESAPCDLL
                     RCSLGYRAPYLCATAREIAADPGWIRRIEALDYPAARRDLMRLMGVGPKVADCVLLFA
                     FRKYEAFPVDVWIARIMQRYYGAGEKEGYEQMSRKGREYFGPCAGYAQEYLYSERERL
                     LSSDPGVQVPSFQLAR"
     gene            complement(12269..13495)
                     /locus_tag="PK154_01485"
     CDS             complement(12269..13495)
                     /locus_tag="PK154_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW09765.1"
                     /translation="MSTGMQKIEWARQYMPVLEAIRERFSKEHPLMGTRVGMALHVEA
                     KTANLVATLVSGGAEVFITGCNPLSTQDDVASALNSMDGVECYAKRGASVDEYYAAID
                     RVLDAEPDVTVDDGMDLIHRIHTKRTELIAKVIGGCEETTTGVHRLRAMARDQQLMFP
                     VIAVNDTPMKRFFDNVHGTGESALTAIMATTNVLMAGKSVVVAGYGYCGRGLARKLHG
                     LGARVIVTEIDPRRALEAHMDGFLVMPMDEAARTGEIFITTTGNTSVITSRHFSMMRD
                     GAILANAGHFNVEIDVKWLEEHAGRIERRDGIDSYQVGTKKVHVLAEGRLVNLASPKG
                     MGHPVEVMDLSFALQALCVLHIAGHGRGLSPGVYPVPESIDEEVARTKLTALGLAIDS
                     LTPSQEHYLASWNEGT"
     gene            13637..14443
                     /gene="hisF"
                     /locus_tag="PK154_01490"
     CDS             13637..14443
                     /gene="hisF"
                     /locus_tag="PK154_01490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPW09766.1"
                     /translation="MVLTRRIIPCLDLKDGRVVKGTHFLSLRDAGDPVELAQRYNEQG
                     ADEVVFLDITASSEHRGTMLEVIGRAADQLFLPLTVGGGIRSVDDIQQILRAGADKVS
                     INTSAVLDPMLITKGSSRFGTQCIVLAEDVRRNYSEHPEATMIPLPDGSRCWYEVVIY
                     GGSKPTGIDAVAWARDAEQRGAGEILLTSMETDGTKNGFDLPVTRAISEAVGIPVIAS
                     GGVGTLEHFYDGFVKGKADACLAASVFHYGEYTVRQVKEYLASRGIPVRL"
     gene            complement(14400..16040)
                     /gene="pheT"
                     /locus_tag="PK154_01495"
     CDS             complement(14400..16040)
                     /gene="pheT"
                     /locus_tag="PK154_01495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPW09767.1"
                     /translation="MAVITLPYRYLERLTRTDRKTILEKISLIGSDVERIEDDHADVE
                     FFPDRPDLFSPEGVARAMRGYLGIETGLPMYRVRPSGISFTVDPNLASIRPVLGAAVI
                     RGVSFDDESIQSIMSLQESLHWAVGRGRSKVAIGIHDLDTVTPPFRYIASPRSRRFVP
                     LDYCEEMSMDEILEKHPKGRDYAKIVRDFPLFPLIVDQDDHVLSFPPIINGERTRVTI
                     DTKNILLDTTGTDVRAVGVAVNIICTAMAEAGATIESVAVGGEEIPTLAPVERSVSVK
                     ECSRLLGIELTAPEMALLLQKMRFGAEAEDTETVKVQIPCYRADIMHDWDLFEDVAIA
                     YGYDRIISQPPPTYTVGKPHTVLVRADRVREVLCGLGYLEVMPFTLTSEEIMYSRMRR
                     PEHPGVLRVLHPISIENTIVRTDLIPLLLEFLAMNRHRELPQRLFTVGDVVHHCTTHQ
                     QAAGVSTHPEADFSEAYATADALLHELSLEYTTVESADPAFIDGRRADIMAGNRKIGM
                     FGEIHPAVLNGFELEHSVAAFEIDLRAVPGYHAMPGTP"
     gene            complement(16043..17485)
                     /locus_tag="PK154_01500"
     CDS             complement(16043..17485)
                     /locus_tag="PK154_01500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW09768.1"
                     /translation="MAELTQNEKRLLAVLGKEERSYATPLAGLLGATPEAVVQWAHLA
                     QEKGLVATERIVARELIYTDEGKRYLETGLPETQLLRGIRQGATVDDLRKHAAFAIGF
                     GQLRKKGLIRVTGSAVEKIPGASTDADEAALKNPAPDDPRTKDLIKRGILAEHETVQH
                     KVSITAEGLALVQSGLDLRAETGTLSRDQIVSGTWRDCNLRRYDVKKLPRRLYPGKVH
                     PYQRIIGEMREILLTMGFEELYGEIVQQCYWNFDALFQPQDHPAREMQDTFYLSETLP
                     LPQGYEKVKAMHIDGGGTSSTGWGGTWKEEKAQQCVLRTHTTCLSIQHLAEHRDPPVK
                     AFAVGRVYRRETIDTTHLAEFEQLEGIVMDEDVTFANLLGILREFYNRMGLESVRFKP
                     SYYPYTEPSLDAEAYVEGIGWVEMGGAGVFREEVTAPLGITCPVLAWGLGISRIAMLR
                     LGLKDLRLLHKSDIQFLRETPVITDAERGI"
     gene            complement(17487..>18423)
                     /locus_tag="PK154_01505"
     CDS             complement(17487..>18423)
                     /locus_tag="PK154_01505"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW09769.1"
                     /translation="EAHAVRGISWEKCDEFGKEYLACLCALGFEGKTYFQSRNNRLKD
                     LAFEAATKVNFSELTAIYGFGADTELAHAESVITQVADILFPQVDSGPAPTVVPVGVD
                     QDPHIRLARGIAHKMRMFTIEEREGYISVRSKNAPEAALNAVKSAFPHAKKYEGHVDI
                     KGGTCTEVAARVREIERAHGGFAFYTPSSTYHIFMPGLTGGKMSSSIPESTISFHESD
                     PVVRKKVMNGITGGRMTLEEQKRLGGEPDRCSLYLLNLFHMVSEDAELAEIRRKCLGG
                     EITCGQCKKDTAERVVAFLKEFREKIDACSDTIRV"
BASE COUNT         4139 a         5095 c         5123 g         4066 t
ORIGIN      
        1 cacgacccgg tcctgttatt gcgcgacttc ccgggcctag cggtgcagcc tgtgccaagt
       61 gccatggtca ggccgccccc ttcacggcat caaaggctgc ttttgccgca gcaatattct
      121 tggtggcgag atcgccccgg aaccgccttt tgacggcatc ttcaagggca gtaaaagcga
      181 tctctccgct tgctgcagca aatgcaccca tcagtgcagt attcgtgatg gggagcccga
      241 tcttctggag ggcgattgag gttgcatcga tggtgatgac cttcacgccc ttcgggacag
      301 gggagtcgat cgccttctcc gtgttcacaa ttgcgatccc tccctctttc atcccgaaaa
      361 agacgttcac atccttgatc agagtactgt cctgtacgat gatatagtcg ggttcataaa
      421 tctggctccg gagacggatc tttttgttgt caaaccggac aaacgcctgg acgggtgcgc
      481 cccgccgttc gacaccgaat gcggggaagg cctgtgcatg cattccccct tcaaatgcgg
      541 cgaccgcaat cagctctgca gcagtgaccg atccctgccc gcccctgcca tgaattcgta
      601 actctctcaa gaaaatccct gaatcgttcc tatgacggct ttgcagccac ctggtgcagc
      661 tgcaaatcct gcaatgatgt tcttccggcg atactgtagt ttctggtacc tattgcaccc
      721 gaagaaataa aaaaattagc atgattgata atgataaact ctgatcccct caaagaagat
      781 ctccccgaat cccttccggg gcaaggttac ctgtcgacgc tgatcccgag aatttcgctc
      841 cctccctgga atacctccct ggcaatgaga gccagccctg acgaggcaca ccattcatca
      901 tagaccgcca cctccctgcc caggagcgct gacacctcgc cggcaatacg aggtcccaga
      961 gacccggcga gcgcaatccg ggcaccaggg ttgaggacga gcagggcagc acattccatc
     1021 gcagagaaga gtgccaccgc ggaaagccgt tcctgttcgg ggagcgtatg gttcacgcct
     1081 gcccggagga atgcctcgtt tgccgtgctg atcccggcat ccacgctccg gatggcatct
     1141 acatcgagcg ccccatgctc aagtcccggt gcaaagatac acgcatcgaa tccgccggca
     1201 atccggccat ccttgatgag caggcttacc gtgtttgaag agacatcgga taccacgaca
     1261 tcgccgccaa ggtcacaaca tacccggtat gcaatcccta tcttttccgg gcttgcctga
     1321 tgggagtaga ccttgaaccg ggtgtcagtg ggagaatccc ggtgcagacc cgggatgacg
     1381 acggcaggaa gaccgctctc ccgtatctgg tcgaacacgc gggttccccc tccgacatgc
     1441 ttgcccgccc cgtccctgct ccgaatcccc cgatccacca ccagttcgat cggggtgatg
     1501 ctgctgatcc catcccccat ggaataggtg agagcaatcc cggatatctc atcgacagga
     1561 cagagtgtcc tgagatcctg ccagcagaag ctccgcgcct cctcccgtgt tattttgaac
     1621 caggactccc cggtggagaa ccggatcgag gtagtgccgt gatcaatacc aatgaacatt
     1681 gttacccatt cctgggcggg ctgtcacgtt tatcattgcg atcgcaagga gcatttccct
     1741 cccggttcat gcggatactc cccgggattc tcttcacgct ccttaccctg tgcagcatgc
     1801 actgccggga acaaatgcct cgatgcatct ctctgaataa cgcttcggcc aacgggcgat
     1861 cgcttccctg ctcgcagatc ttgatcgggg aggattcatc caaccactcc tgccggcacc
     1921 aaacaggcag cccgtttgag catcgaatct tgaaaccaca gttttagtag gagagagaag
     1981 agaattttaa aggtgaatgc acagaacggg ggcggagtct gaaagcggtc gttacaggag
     2041 gggccgggtt tatcggctcc cacctggtgg atgccctggt cgaacggggc gagtcagtca
     2101 ctgtcatcga caacctctcc accggagata tccggaacat cgcagggcat atcgattccg
     2161 gagcagtcag gttcatcaaa cacgatctcc tccttgacgg atggcaggag cacctcagag
     2221 gtgccggccg tatctatcat atcgctgccg acccggatgt cagggggagc gctacgcacc
     2281 ccgaagaagt gtacaccagg aatgttcatg cgacggtgcg ggtactcgag gccatgagga
     2341 gggccgggtg cgctgagatc gtattcacct caacatcgac cgtatacggt gaggccgctc
     2401 tcatcccgac ccctgaggaa tacccccgga tggagccaat ctctatttac ggggggacaa
     2461 aactggcctg cgaggcgatg atctctgcat atgcccacac gttcgggtgg aagtcatggg
     2521 tattccggtt cgcaaatatc atcggcgaac ggagcaatca tggggttatc tgggatttca
     2581 tccataaact ccgccagaat ccccgggaac tcgagatcct tggcgatgga acgcagacaa
     2641 aatcatactg ctccgtgccg gcatgtgttg atgccgtgct cttcgccgtg gcacatgcca
     2701 gtgccccgtt cacctgtctc aacatcggtt cggaggactg gatcgatgtg accaccattg
     2761 catcgatcgt agccgatgag atggggcttt cggacgtatc atatcactat acggggggag
     2821 accgcggatg ggtaggagat atcccgcgga tgctcctttc tattgaaaaa atcaggtcac
     2881 tgggctggag atatgaagtg acctcgcagc agtcagtccg ggaagccgcc cgggcactgg
     2941 tcctggagac gggatacctg gtgaggtgag gggcatgagg gaaaggcaga agaaatacct
     3001 ggtcattctc ggcgatggaa tggcggacga gcctctggcg gaactcgggg ggaagacccc
     3061 cctggaatac gccaggactc cgaacatgga caggatcgcc tgcgagggga cgatgggact
     3121 ggcgcggacc attccggaag gattcgaggc agggagcgat attgccaacc tcgggattct
     3181 cggttatgat ccccgctcct gctataccgg aagggggcct cttgaagcgg cgagcatggg
     3241 cctcgagatg gctccgggag acattgctta ccgctgcaat cttgtgacgg tcgttgacgg
     3301 aaggatggcc gacttttcgg caggccatat ctcgagcaga gaaggagcat ccctttttgc
     3361 atcgctttca gaacatctcc cggatatctc cctctctccc ggcatctcct accggaacat
     3421 tctggtggtg catggggggg gaggggcaac cacctgtcca ccgcacgata tcgtgggtga
     3481 gctgatcgaa ccctcccttc cctccgggaa agatgccggg attctgcggg agtgcatgga
     3541 gaagagccgg gaggtcttcg cagaccaccc ggtgaacagg gcacgcatct cggcacagaa
     3601 atcccccgcg acccagatat ggccatggag cggggggaaa aagcccaggc tcatcccatt
     3661 ccggaccctt tttggcaggg aggcagggat catttctgca gtggacctgc tccaggggat
     3721 cgcgaggctt gcggggatgg aagtgattcg ggtacctggg gcaaccgggt accttgatac
     3781 ggattaccag gcgaaggctt cctctgcatt ggaggcactt gagcgcctgg attttgtcta
     3841 cgtccatgtt gaagcgccgg atgaggccgg tcaccttgga aacgtctggg aaaaggtgaa
     3901 ggcgatcgag cggattgacg agctgatcgg gaccgtgatg gaggggtttt ccggggttat
     3961 tgcagtgctg cccgaccatc cgacccccat acgaatcaag acccatacct ccgatcccgt
     4021 tccgttcgcg atatggggca aggggcggga tgccacgacc tctttctcag agaaagaggg
     4081 atcgcgcggg ggatatggtg ttattgatgc cccccgtttc cttccccttc tcttctcata
     4141 actgcttttt ccgctcatga taaaccatat cagagcgggt ggagaatatg atctcatggg
     4201 tggccataag accctcacta ttggcgtcac cgtgaatctg gaacactatg agaacctccg
     4261 ggttgaggtc agcggggacg cggactcccc tggcgacgcg gaagagctca cccggtttct
     4321 caccgcgata ctcggcagct ttgggaggaa tgacccggct acggcagagc ggatcgatag
     4381 ttaccggagg cgcgtcctcc cgggatctgc cagcagcgtc agtgcaggag gcgagggggc
     4441 agcgatcctg ccccctgcgg ctgaatctgg cggggaaaaa cagacggaat ccgaacctgc
     4501 cgaatcccgg aaggcacaag cctctgcccc ggtaccccct gcaggtgccg ccgcgtcagt
     4561 ctccggccca acctgtgact cgtgtgggac acctgtcagc cctgcagagc agaagatgag
     4621 tcagctcttc accagccgaa ttttgtgcag agcatgtatg aagaacctgt gagactcaga
     4681 atacctgaga ggcgatccgg atgaaaaaga atctcctgat gtgggatgag accctctttc
     4741 gtgacccgga ggtctttgag atcgattacg tcccggaaca gttcaaccac cgggagagcc
     4801 agatacgcga actggctttc cagatccatc ccggtctcag gggggggcgt cccctgaaca
     4861 ccatctgccg gggactgccg ggaacgggca agaccaccag cgtaaggaag atattttcgg
     4921 aaattgagga aaatacaaag aaactcgtgc cgatttacat caactgccag attgacaaca
     4981 ccaagtttgc catattctcc cagatctacc ggcgactctc cggtcacctc ccccctgcct
     5041 ccggcacctc tttcaagcag gtcttcgacg cgatcgcccg aatgctcctg aaggaagagg
     5101 tggtacttct ggtggcactc gacgatgcaa actacctcct ctatgagaat gagatgaacc
     5161 gggtcctcta ctccctcctc cggtcccatg aagcgtatcc gggaacccgt atcggggtga
     5221 ttgtgatcat cagcgacatg gatgtcgacc tctcgcaggc ggtggatgca cgggtgtcat
     5281 cagtgttccg tccctcagag gtatattttc ccccctatag cgaagatgaa gtgcgggaga
     5341 tcgtcaggga gcgcgtttta cagggcctgt atccgggctg cctccctgag gagatgttct
     5401 ctctggtcgt tgaacagacc cagaaatccg gcgatctcag ggttggtatc gatctcctca
     5461 agagggccac cctgaacgcg gaacgaaggg ccagtcgtgt catcgaaagg gatgacatct
     5521 gccaggcgta cgatgtctcg aagtacctcc acctctcctt ctcggtaaag tccctcaagg
     5581 acgaggagaa ggaggtcctt cgggcacttg cgaaccacag cacgggcgat tcagagatga
     5641 atgcaggaga ggtcttccga gcggtcaaag agaccgtccc gatagggtat acacggtatt
     5701 atgagatcat caggaaattc gacgcgatgc ggctgatcaa cctccattac cgcgatggaa
     5761 aagggaggac gcgggtgatt agcctccgct atgatccggc aaaaattatc aattatctgg
     5821 gctgagttga tttttaaaac acatcatttg aaaaatctta tctctttggc aaccatctct
     5881 tatggtaaag ggggtattcc acgtgctcag tgtgaatgaa ttagctctcg aaatttttga
     5941 gaacctggca gaatatgcgg aagagttcaa tgctgcatac catgaactcg acaacggtgc
     6001 acggatagtc gattgcggtg taagcacaag aggaggatat gctgccggca gggcttttac
     6061 cgagatctgc atgggtggcc ttggagaagt gaacttcagg atgggtcata tcaaggagtt
     6121 cccggtaccc ttcatcgatg tcttcaccga ttatccggcg atatcctgtc tcggtgcaca
     6181 gaaagcaggc tggacgatca agcagaacaa ttattttgct atgggcagcg gccctgcacg
     6241 ggcgctctct ctcaaaccaa aacacaccta cgaggtgatc gagtatgagg atgattttga
     6301 ctccgcaatt attgcgctcg aaagtgatca cctccccaac gcaggagtca tggagaagat
     6361 agccgaggag tgtcaggttg atgtcgctaa cctgtgtgcg gtggtcgccc ccacagcatc
     6421 gcttgtcggt tcgatccagg tggcaggcag gtgtgtcgag acggccgtct acaagctgaa
     6481 cgagctcggt tttgacacca agaagattgt tgccgggttc ggcacagccc cagtgccccc
     6541 ggttaagaaa gatgccacac gggcgatggg aacgaccaat gatgcaacca tctatcacgg
     6601 caggatcatg ctcacgatga aggcgccgga gatcaaagag tacctggaaa aaatcccgag
     6661 caacaagtcg aaagggtatg gcaaaccgtt ctatgagatc ttcaaggagg cacagttcga
     6721 cttctataag atcgacacct ccctcttctc ccccgcagaa gtggttatca acgaactcac
     6781 cgagggagtg gtatatcact gcggtgcagt aaatccggat gtcaccctca aatcctttgg
     6841 tctcatctga atcaatactt ttttctgtca tggcggagca tgggcccgtt tcagggcagg
     6901 cggcctggga ggcagggtac cggaagagcg ggagaaggta tggacgtgct ccgcctgatc
     6961 tccccccgtt tcctcccggg acacggattc ttgaggccgg gtgcggggac gggaagtcac
     7021 ttcttgccat gaccggaagg gggtgggaca ttgttgccat cgatttttcc agggaggcga
     7081 tcaggatcag ctcccgtcag aacatgctgg agaggggccg gttcgtccag gcagatgccg
     7141 cggctcttcc tttcacggac gggagctttg atgtggtgtc catatcacat ctcctcgggc
     7201 atctcccgga ggagcacagg tcccgtgcgg cgtcagagac agaacggatc cttgttcatg
     7261 ggggattcgc gtatatccgg gtcttctcca cgcgggattt cagggaggga aaaggaatcc
     7321 tcaccgaacc acatacttac ctgaggggtg atgggattat gacccactat ttctcagaaa
     7381 aggaggtcga gaccctcttt ccaacacttg tccgcatgga gctgtcgacc tctgattggt
     7441 ccatgaggat aaagggtgag cacatgaacc gttcagaaat catcgcactg ttcagaaaat
     7501 gataccctgc ccgggtccag ccctgagatc agctgatgtc aggcaccgtc aagggatgga
     7561 ccggaggggg gataatcgcc tttttgtgaa ttcaacgatg aggaacaccg caggtcctcc
     7621 caggaacatg accggtaagc cgtccgttcc tgataggtgt gatgcgaccg tatcccggga
     7681 ttagtcagca attttaatta aggttaccgt cgaatcagta tagtccttcc gggctcgtgg
     7741 tctagctggt tatgacgtcg cccttacacg gcggaggtca ggagttcgaa tctcctcgag
     7801 cccatctgtc tgattttttt catgtgctac gggacgagga ttgagtgccg gattggtggt
     7861 gcggcacccc cctttttttt ggaaaaaaat gatcccatat tatgcgttcc cagcctgatc
     7921 tgacacgagt gcagcggagc tgggctgatt ggagcgcagg gaggtttttt ttgaaagaac
     7981 cttaaatatg gattattttt ggattgtaag ccaaaattgg tgttaaaaaa atttaagatg
     8041 aatggcgggt tatttcaggc aaataagtgt gatctggctg acgccatcaa aaatatgatt
     8101 aaaatatgag aattttacca aatgatttat atagaagaaa ataaaatcca ttttttggtg
     8161 caccgattcc gcgggcgaag cctgcccgga aaagcactaa acaatcaagg tggcatctat
     8221 attggcagta ctccaggcat tagcaatgac ggggcggttg ttcggtacac caatttgaga
     8281 tacaacattc tgataaaaaa ggtatcggat cgaatcgggc aggccgaccc atccgcgatc
     8341 ttttttttgt aattttattc acatcaatac cgatttcacg aagaaataat tcccgttttt
     8401 cccttttggt gatagaagcg ggataagaca ctggcagggg ccaattcctt gccgatcgaa
     8461 accggccagg gagtactgat ccgatcatat ctcccggatc gtggcccctt atcgtatccg
     8521 gtgattctct atgcgatgcg ctccctttca ggtccctgga tgaacgattt cgacgagcgt
     8581 tatctcaaat accatatcct gtcccgcaag gggattgttg agatcgactg ttaccatggt
     8641 atcagtggca ttaaaaaacc tcatgtatcc tgggctgcca tctgaattta tccagcggta
     8701 cccggatccg ataccaggat actggaatcc gagaagattt cccatggact cctgatcctg
     8761 taaaaattcg ttcacctctg cctgatctac tctgaaaacc aatgatttgt tataaaggcc
     8821 ataccctttt tccgggggga tggtaacaat ttttgtcgtt ccgggagtca tgccgatcac
     8881 tgcttcatca aacccggaaa tgacctgccc acttcccacg gtgaattcca gggggggtgc
     8941 tgtttacgtt ggtatcaaag atcgtcccat cctggaatga tgcagtatac ctgaccttga
     9001 cggtatcatt ggtctttacc ccctcctgca cgggagctga acagccagcc accagaagga
     9061 aagcataaca gatgaggaga gcagcggtga gccatactac ggaatccctg cttccaatcg
     9121 tgagtggatt cgttttcata gttatacata atcccccgca aaagagtata agatatcctg
     9181 tgcacgccac agccttgctc tcactgatgg ctcgtttacg attacctggc cgggcatccc
     9241 tctcggagtg ccccatcgaa tcatttttat tggaatgaac gcgaactaca ctggtattca
     9301 gtgggcatca tgacagccga aggatacaga gaggcggagg ttgccggccg gaaggtcaag
     9361 tgggatccgg aacaactgcg aaggatcaaa gaacaccctt gtttttcaga acaggcctgc
     9421 catgcattcg ggagatgcca tctcccggtt gctcccaggt gcaacatcca gtgcaactat
     9481 tgcatcaggg attttgactg cgtaaatgag agccgcccgg gggtcacgac aaaggtcctc
     9541 gacccggacg cggcaatcga catggtccgg aaggtgatgg agaagttcaa ttacatcaag
     9601 gtcatcggga tagccggtcc tggtgagccg ctcgcaaacg aaggcacctt tgaaaccctc
     9661 cggagactgc acaaagaatt cccgagcgtt atcaagtgca tcagcaccaa cgggctcctt
     9721 cttcccgaca agatcgatct cctccatgaa tatgatgtgg gaaacatcac cgtcacgctg
     9781 aatgcaattg atcctgaaat cggggcaaag atctaccagt tcattgatta caaagggaag
     9841 aggtataccg gcgtagaagg ggccaagatc cttctcgaaa accagttgaa ggggatcgag
     9901 atggcagtag accgccacat gatcgtcaag gtcaatacgg tctatatccc tggtatcaac
     9961 gaagatcata tcccggagat tgccaaaaag gtaggtgaaa tgggagtata caactttaat
    10021 ctcattcccc tgatcgccca gtacaaattc gctgacatca ccccgcccac tcccgagatg
    10081 aagagaacga tgcaggatga atgttcaaaa tacgtgaaac agatgcgtca ctgccagcga
    10141 tgccgggcag atgcaatcgg acgtcttggt catgacgttc agtcgtgcat gtattcagag
    10201 tagcccattt ctttttgtcc cccccatttt ttcctctgat tcattccaat caactcgtgg
    10261 cgagaggata ccattcacca tacgaccgga atacagggac cagggagaaa gagagagcaa
    10321 tgagaaagga gggagggatt tttgcctcag ctgagagggg cgtaatcggc aaggatcgca
    10381 tcaagacggc tgatcaccgc aatgagtgga tccggtatcc cgccggtctc ggttgtgacg
    10441 gtcttcccat cgtgggtgat ggtatacgag aaataatcag ccccgggaga aggtgccgga
    10501 tacgaagagg caagcgaggg aaaatcagca tcggaaagga gactcttcat ctctgaaagc
    10561 cggtcttgcg ggagggtgaa ttctcctttg gcgattctcc gtgaatagac cgcctgtcca
    10621 ttatcgaaaa tgacgagcct gtcatcaaag gccgcgatcc ccccggttct tgaataatcc
    10681 accaacacct tctgagttgt atgattatca gagggaaccg gaggtctcgt gacgcatcct
    10741 ccaatgaaac tcaggaccac cagacatgaa aaaaacagga taatccctgc tcgtgaacgg
    10801 gatgaaagag cagcgatggg ggttgtttgt cttgcatgat cattgtgcat tgattctcat
    10861 tttacctctg ggggcatata tgactattcc agggagatgc gggaaaagaa acggagtggg
    10921 tgacggtcaa atctgaagga catccccctc ctcacccttc gctcccttcc gggcgggcac
    10981 taatgactcg agagaagaga gcaggacccc gaccttctcg ttgttgaaga tctccctgaa
    11041 cgcgttgagt tcagcctgcc ccatgatcat cacccctttc ttcttcatcg gggtcccctt
    11101 atccgtcagg ggattgatct cgattgcgat agaggcaggt ctcgatttgg tctgcgggag
    11161 tttcaggatc gtaacgcccc ggatggaggt cagcttccgc tcccagtcgt cgccgttctc
    11221 caggaaaaca cgtaaggtat caaccagttc cataccagaa catcagcggc atgggattaa
    11281 aaggatacgc gtgcagggtg aaataaccag accggaagga tcaccggata tcctgtcatc
    11341 cccgtcttcg tctcccgctc ctgacatgac aagagggccc gatacctctt atttgaccag
    11401 aacacatata ctccccatca atgccacgta tcacccttgg agagcattgt cctttctctc
    11461 tcgacctgac cctcggatgc ggacaggtgt tccgctggca gaaatccggc gaatggtgga
    11521 ccggagtggt ggagaaggac ctgatcagga tccgccaaaa aggacgaacg ctgatatggg
    11581 aggggtcaac acaggagcgg atacgtgact atttcgggct cgatcatgat ctcggccgga
    11641 taatcgagtc ggtggaccgc gatccggtca tccacctcgc cgtcgccaac agtacagggc
    11701 ttcggctgat acaccagccg ccctgggaat gccttgcctc ctatatctgt gccacctatg
    11761 ccaatattcc cggcattagg agaaagatcg ccctcatctg tgatacattc ggcgatgaga
    11821 tctccagcag gaaggatttc agactctcaa cgtttccttc cgccgatgta ctggccgaat
    11881 cggccccctg tgatctgctg cgatgcagcc tggggtaccg ggcaccctat ctgtgtgcaa
    11941 cggcaaggga gattgccgcc gacccgggat ggattcgaag aatagaggcc ctcgattacc
    12001 ctgcagcccg gcgggatctc atgcggctga tgggcgtcgg gcccaaggtc gcagactgcg
    12061 ttcttctgtt tgccttccgg aaatacgagg catttcctgt cgatgtatgg atagcccgga
    12121 tcatgcagcg gtattacggg gctggggaga aagaaggcta cgagcagatg agcaggaagg
    12181 ggagggaata ttttggaccc tgtgccgggt atgcacagga atacctgtat agtgaacggg
    12241 agaggctcct ttcatcggat ccgggggttc aggtcccttc gttccagctc gccaggtagt
    12301 gctcctgcga aggagtaagg gagtcgatgg cgaggcccag ggcggtgagt ttcgtccgcg
    12361 caacctcttc atcgattgat tccggtacag ggtagacgcc ggggctgagc ccgcggccat
    12421 gaccggcgat atggagcacg cagagcgcct ggagtgcaaa actcagatcc atcacctcca
    12481 ccggatgtcc catccccttc ggactggcaa ggttgacgag tctcccctcg gcaagcacat
    12541 gcaccttttt tgtcccgacc tggtagctgt cgatgccatc cctccgttcg atccggccgg
    12601 catgctcctc gagccacttc acatcaatct cgacattgaa atggccagca tttgcaagta
    12661 tggcgccatc ccgcatcatg gaaaaatggc gggaggtgat aacagacgta ttccctgtcg
    12721 tcgttatgaa tatctcgccg gtcctggccg cctcgtccat gggcatcacc aggaaaccat
    12781 ccatatgcgc ttcaagtgca cgccgtggat cgatctcggt cacgattacc cgtgcgccaa
    12841 gaccatgcag ctttcgcgcc agtccccgtc cacaataacc atacccggca acaacaacac
    12901 tttttcccgc cataagcaca tttgtggtgg ccatgatcgc ggtgagggca ctctccccgg
    12961 taccatgtac gttgtcaaag aaccgtttca tgggggtgtc attgaccgct atgacaggaa
    13021 acatcagctg ctgatccctg gccattgccc tgagtcggtg gacaccggtg gttgtctcct
    13081 cacagccacc aatcaccttc gcgatgagtt cggtcctttt tgtatggatc cggtgaatga
    13141 ggtccattcc atcatcaacg gtcacatccg gctcagcatc gagcacccgg tcaatggcag
    13201 catagtattc atcgactgaa gcgccccgct ttgcatagca ttccactccg tccatcgagt
    13261 tcagcgcaga agcgacatca tcctgggtgg agagtggatt gcacccggtg atgaatactt
    13321 ccgcgccacc agaaacgagg gttgccacca ggtttgccgt ctttgcctcg acatgaaggg
    13381 ccatcccaac cctggttccc atgagggggt gttcttttga aaagcgttcc ctgatcgctt
    13441 ccagtaccgg catgtactgc cgggcccatt caatcttctg cattcccgta ctcattatac
    13501 cccacaccag aggtgacacc agacctggct ggcgcctccc atccgtctgt tcctgcctat
    13561 aggttccccg cacaccagat aaactgttac cggtctttca aaaggatgat gatgaaggcg
    13621 gtagagaagc tggtacatgg ttctgacaag gcgcataatt ccgtgtctcg atctcaagga
    13681 tggacgcgtg gtgaaaggga cccatttcct ctccttgagg gatgcgggcg atcccgtgga
    13741 gcttgcacag cgatacaatg agcagggggc cgatgaagtg gtatttcttg atatcactgc
    13801 ttcaagtgaa caccggggta cgatgcttga ggtgatcgga cgtgccgccg accagctctt
    13861 cctccctctg accgtgggag ggggaatcag gtctgtcgat gatatccagc agattctcag
    13921 ggcaggggca gacaaagtca gtatcaatac gagtgcggtc cttgatccca tgctgataac
    13981 caagggatct tcccggtttg gtacgcaatg catagtactt gccgaggacg tcaggagaaa
    14041 ttattcggaa catccagagg caacgatgat cccgctcccg gatggttccc gctgctggta
    14101 tgaggtcgtg atttacggtg gaagcaaacc aacgggaatc gatgcagtgg catgggccag
    14161 ggatgcagag cagcggggag cgggagagat actcctcacc agcatggaaa cggatggaac
    14221 aaaaaacggt tttgacctcc ccgtcaccag ggctatctcc gaagcggtcg gcatccccgt
    14281 catcgccagt ggcggtgtcg gcacgctgga gcatttctac gatggatttg tcaaggggaa
    14341 ggcagatgcc tgcctcgcgg caagcgtctt ccattacggg gaatataccg tacgccaggt
    14401 caaggagtac ctggcatcgc gtggtatccc ggtacggctc tgagatcgat ctcgaaggca
    14461 gcaacggagt gctcaagctc aaatccgttc agtactgcag gatggatctc cccaaacatc
    14521 ccgatttttc ggtttcctgc cataatatca gctctgcgcc cgtctataaa tgccgggtca
    14581 gccgattcca cggtggtata ttcgagcgaa agctcgtgca ggagtgcatc agccgttgca
    14641 tacgcttctg aaaagtcagc ttcagggtga gtgctgacac ccgcagcctg ctggtgggtg
    14701 gtgcagtggt gcacaacgtc ccccacggtg aaaagacgct gcggcagctc ccggtgccgg
    14761 ttcattgcca gaaactcgag cagcagcggg atgaggtcag tcctgacgat ggtgttctcg
    14821 atgctgatgg ggtgcagcac acggaggacc cccgggtgtt caggccgccg catccgcgaa
    14881 tacatgatct cctcgctcgt gagggtaaac ggcatcactt cgaggtatcc aaggccgcag
    14941 aggacctctc tgacgcggtc tgccctgacc agtacggtgt gcggttttcc aacggtgtac
    15001 gtgggaggcg gctgggagat gatccggtca tatccgtaag cgatcgccac atcctcgaaa
    15061 aggtcccagt catgcatgat atcagcccgg tagcagggga tttgcacctt caccgtctct
    15121 gtatcctccg cttcagcccc gaagcgcatc ttttggagga gcaaggccat ctcgggggcg
    15181 gtcagttcaa tcccgaggag acgggaacat tcctttacac ttactgaccg ttccaccggg
    15241 gcaagggttg gaatctcctc ccccccgact gccacgctct cgatcgtcgc ccctgcctct
    15301 gccatcgcag tgcagatgat attcacggca accccaaccg cccgcacatc ggttccagtg
    15361 gtatcgagga ggatattctt cgtatcgatg gtaactcgtg tccgctcccc attgatgatg
    15421 ggggggaatg agaggacatg atcgtcctgg tcaacgatca gcgggaagag cggaaagtcc
    15481 ctgacaatct tggcatagtc ccgccctttc ggatgtttct ccaggatctc atccatgctc
    15541 atctcttcac agtaatcgag cggaacaaac ctcctgctcc ttggtgatgc gatataacgg
    15601 aacggagggg tgaccgtatc gaggtcatga atgccgatag ccaccttgct cctgccgcgg
    15661 cctaccgccc agtgaaggga ctcctggagc gacatgatgc tctgaatcga ctcgtcatcg
    15721 aacgataccc cgcggatcac agcggctccg agcaccgggc ggattgaggc caggttcgga
    15781 tcgaccgtaa acgagatgcc tgatggcctg acccggtaca tgggcagacc cgtctcgatc
    15841 cccaggtagc cccgcatcgc cctggcaaca ccttccggag agaagagatc agggcggtca
    15901 gggaagaatt cgacgtcggc atgatcgtct tcgatcctct ccacatcgga gccgatcagg
    15961 gatatcttct cgaggatggt cttccggtcg gtccgggtga gtcgctcgag ataccggtag
    16021 gggagggtga tcactgccat ggtcagatcc ccctctctgc atccgtgatg acaggggtct
    16081 cccgcaggaa ctgaatatcg ctcttgtgca ggagacgcag gtcttttaag ccgagacgga
    16141 gcatcgcaat cctgctgatg ccaagacccc atgcgagcac ggggcaggtt atcccgagcg
    16201 gtgcggtcac ttcctcgcgg aagaccccgg caccccccat ctcaacccag ccgatccctt
    16261 ctacatatgc ttctgcatcg aggctcggct cggtgtaggg ataatacgag ggcttgaacc
    16321 ggacgctttc cagtcccatg cggttgtaga actcccggag tatcccgagc aggtttgcga
    16381 atgtcacgtc ctcgtccatc acgatccctt cgagttgttc gaactcggca aggtgcgtgg
    16441 tatcaatcgt ctcgcggcgg tatacacgcc ccacggcaaa tgccttgacg ggcggatccc
    16501 gatgctctgc gaggtgctgg atggagaggc aggtggtgtg ggtccggaga acgcactgct
    16561 gagccttctc ttccttccag gtccctcccc atccggtcga tgacgtcccg cctccatcga
    16621 tatgcatggc cttcaccttt tcgtagccct gggggagggg aagggtctcg cttaaataaa
    16681 aggtgtcctg catctcccga gccggatggt cctggggctg aaagagagca tcgaagttcc
    16741 agtagcattg ctggacgatc tccccgtaca gttcctcaaa ccccatagtg agaaggatct
    16801 cacgcatctc accgatgatc cgctggtacg ggtgtacctt cccgggatag agcctccgtg
    16861 gcagcttctt cacatcatag cgcctgagat tacaatccct ccatgtgcct gatacgatct
    16921 ggtcacggga gagggtcccg gtctctgccc gaaggtcgag ccccgactgc accagggcca
    16981 gtccttccgc ggttatggaa accttgtgct gcacggtctc atgttcagcc aggatacccc
    17041 gtttgatcag atcctttgtc cttggatcat caggagcggg atttttaaga gctgcttcat
    17101 ctgcatcagt agaggcaccc gggatctttt ccacggcaga tcccgtcacc ctgatgaggc
    17161 cttttttcct gagctgcccg aaaccgatcg cgaaggcagc gtgtttgcgg aggtcgtcaa
    17221 ccgttgctcc ttgcctgatc ccgcggagga gttgtgtctc gggcaaaccg gtctcaagat
    17281 accgcttccc ttcatcagta tagatgagtt ccctggcgac gattcgttcg gtcgccacca
    17341 gccctttttc ctgtgcaagg tgtgcccatt gcacaaccgc ctccggagtg gcacccagca
    17401 gtccggcaag aggggtggca tacgacctct cctcttttcc aaggacggcc agcaggcgtt
    17461 tctcattctg cgtcagttca gccatgtcac accctgatcg tgtctgagca tgcgtcgatc
    17521 ttctccctga actctttcag gaacgccacc acccgttcag cggtgtcctt cttgcattgt
    17581 ccgcacgtga tctccccgcc caggcatttg cgccttattt ctgccagttc cgcgtcttcc
    17641 gataccatgt ggaagaggtt gaggaggtac agggagcacc ggtcgggttc accaccgaga
    17701 cgcttctgct cctcgagggt catcctcccc ccggtgatgc cattcatcac cttctttcgg
    17761 acaaccgggt cagactcatg gaacgagatg gtactttcgg ggatgctcga ggacatcttc
    17821 cccccggtca ggcccggcat gaagatatgg tacgttgagg agggggtata gaaagcaaat
    17881 ccgccgtgag cccgttcgat ctcacggacc cgggcagcga cctcggtgca cgtccccccc
    17941 ttgatatcaa catggccttc atactttttc gcatgcggaa atgccgattt gactgcattg
    18001 agcgcagctt cgggggcatt ctttgaacgg acactgatgt atccctcgcg ctcttcgatg
    18061 gtgaacatgc gcatcttgtg agcgatgccc cgtgcaaggc ggatatgggg gtcctggtcg
    18121 accccgaccg ggaccacggt cggggcaggg ccgctgtcca cctgggggaa gaggatatcg
    18181 gcaacctggg tgatgacgct ctccgcatgg gcgagctcgg tatcggcccc gaatccgtaa
    18241 atagccgtga gctccgagaa gttcaccttg gttgcggcct cgaatgcaag atctttgagc
    18301 cggttattcc tgctctggaa ataggtctta ccttcgaacc cgagggcgca caggcaggcg
    18361 agatactcct tgccgaattc gtcacatttc tcccaggata tgccccgcac ggcatgggct
    18421 tcc
//
LOCUS       DAOOCO010000012        17898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2790_length_17898_cov_5.319061, whole genome shotgun sequence.
ACCESSION   DAOOCO010000012 DAOOCO010000000
VERSION     DAOOCO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_17898_cov_5.319061"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..542
                     /locus_tag="PK154_01510"
     CDS             57..542
                     /locus_tag="PK154_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW09770.1"
                     /translation="MQVGIGSRTGAGILDLANLRIANAPPGSTGRMVIDRLLTEQTIN
                     PDQVLGNGNSVKTEAAVISVVINGRADAGICSAVLAREAGLAFVPITYESHVLVARNE
                     SIEDERIHSLLQILQSPEFRDSLDYCYDTTLTGKVEECNTGSWQEVSFLDNWISDSGG
                     G"
     gene            complement(684..893)
                     /locus_tag="PK154_01515"
     CDS             complement(684..893)
                     /locus_tag="PK154_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09771.1"
                     /translation="MKIYVRERMKVREGIKQPMFRIVAVTGGTVQIELTHFRKFEIEQ
                     IAKDVGAEVIYFEEIPDEEKEKQKH"
     gene            1002..1814
                     /locus_tag="PK154_01520"
     CDS             1002..1814
                     /locus_tag="PK154_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HPW09772.1"
                     /translation="MKRDRFEVANKLQGESLVRKGLLKAYEGRSQLRMMPDLNVVKIG
                     GHGIIDYGKEVLLPLLTEIGELSRENQILVVTGGGVRVRHILDLGLDLGLPTGVLAEL
                     AGKISEQNAIMVSILLSKYHGTRIHTGDLLELPMLMNLGVLPVIHGTPPYGLYEQPAH
                     IGSIPPNRTDTGAFLAAEVLGAKNLILVKNVDGLFTANPFTDTTAEIIPDITAAELLA
                     MEMEDMVLEPRVVELMMNAKHVHEVKIINGHEPGNLRRAIAGERIGTVIRAV"
     gene            1841..2962
                     /locus_tag="PK154_01525"
     CDS             1841..2962
                     /locus_tag="PK154_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HPW09773.1"
                     /translation="MPQDSKPPLRFGIGELAGSVGDFGTILPLSLALAATGALGIGPV
                     LLFLGIWFIITGYYYRYPVPVEPMKAIAVIAVAAGMSGGEIAAAGYILGAIFLLIGFT
                     NVLEIIERYIPLPVVRGIQLGLALILLKTAVVYLIPDPGHFLLGAMIISGGFLVAYHF
                     RVPDLSSIAVIIVAIAAGILAQGFPPITTLSLSLGAIPTGSEIIASLRELVLPQAILT
                     ITNAILATSLLAKDLFSADIRPKKLSKTIGLMNLTSIPFGGMPMCHGAGGMAGQYRFG
                     ARTGGANIYAGIFLIGAALLFASTAWVGLIAPGFYAALLLFVAMELGRHGLKTNSYLV
                     TLPTAILSLLSSMTVAFIAGMCLAYGIPSIRRWFAEKSR"
     gene            complement(3141..3644)
                     /gene="tsaA"
                     /locus_tag="PK154_01530"
     CDS             complement(3141..3644)
                     /gene="tsaA"
                     /locus_tag="PK154_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPW09774.1"
                     /translation="MEEGILFRPIGIIHTPFQEPAGMPIQPTGGKEIEGTVEIFPEFA
                     DGLLDMDGFSRIILIYHFHRSGPCRLHLIPFLDTRPHGVFATRAPNRPNPLGISIVSL
                     IACKDTSLHIRGVDMLDNTPLLDIKPYLPECDAFCEERTGWFPSGESRFSATLSDDRF
                     TGHERIN"
     gene            complement(4011..5954)
                     /gene="wtpA"
                     /locus_tag="PK154_01535"
     CDS             complement(4011..5954)
                     /gene="wtpA"
                     /locus_tag="PK154_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HPW09775.1"
                     /translation="MALKKQISIFIILTLLVVGFATFAGCTTTEPGSGTPTVTPTPSE
                     TGNATTVPTTVPPTPVGPKTKVLLATTTSLYDTGLLDYLKPKFEEQYNAELLITSQGT
                     GKAIEVAKKGDCDILAVHSPSQEVTYMEDGAGLNRRCFAYNYFIIVGPANDPAGVKGM
                     TPEQAFTTILTKGKANTPGVMFVSRGDNSGTHSAEKAVWSSAKYNYTKDVQKSGNWYI
                     EAGRGMGETLQLASEKGAYTLTDEGTYLAYQGDLDLEPVVSEGAILLNVYSVITVYND
                     KTTPEKIEMANNFVNFLISPQTQADIENYGKDKYGKNLFSAMNGDCAKFNCVCTGEAT
                     ALRPMTVFNAGSLNAPFAKIETAYESQFPLVDAQLFGGGSVTMIEKITKQGKKADVLA
                     SADAYLIPTLMYPTYADYYVTFAKNHMVVVYTNQSKHSDTITSDNWYEILNTPDVKYV
                     ISDPNTDPAGYRAVMSVQLAERVYGVDTIFSSLLASHSKMTRTFSDGKYTIDVTKPTP
                     DGKLVIGKSGPDVVTILKEGKADYAIEYSSVAIQNGLPYVELPVGMDLSAQENADSYA
                     TVKVKRISGTTTATETGTPIIYAVTVPKNARSPDLGLEFIKILISQAGQDILAADGQE
                     VISPPLGYGNVPAALKESGVVMA"
     gene            complement(6184..6675)
                     /locus_tag="PK154_01540"
     CDS             complement(6184..6675)
                     /locus_tag="PK154_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09776.1"
                     /translation="MAVLVSALLIQGGSADHLTIGASPELSAIGERLVLSGSTDAKGT
                     IAVFLLVSGPGLDKRGVCLENLNLPAGHGYFTSAYVNPDGTWRYIWDTNYLAGRLIPG
                     TYTVYVVNLPLGLPHSGSVSFAKTNVTFTKIPDPGLGIDAVTLCIAGMTGAGLLFLCR
                     RKG"
     gene            complement(6999..8297)
                     /gene="brxD"
                     /locus_tag="PK154_01545"
     CDS             complement(6999..8297)
                     /gene="brxD"
                     /locus_tag="PK154_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HPW09777.1"
                     /translation="MPILADHGDKRKTGSVNIINALRRGTVPAGGLERIAVGIDVEEG
                     VIASQLDYVLNGGGDLKFIRGDYGSGKTFLVARALEIAREKNFVTAHVVISPASPLYK
                     LKGMYQQICATLRSREEEHAMKAVIDTWLFSIEERLLKVSDHPLPEDSLEIATLKEIE
                     TALAGISEMNSSLAAALRTYYRANNAGDFQTAQSAIGWISAEPNIGREFKAKAGIRGD
                     VDETFALIFLRAIVRIIVNAGYAGLAVAIDELETTQTLPRNQREKGYTTLRLIIDALD
                     RGEMPYCYFFFTGTPAFFEGPRGIRSIPPLADRIGVTDSGEFRNPRQPQISLSKFDAK
                     KLEQVASKVIIIYSEAYGEVDRNRVSHRFIRAMINRLTGKFGGRIDVIPRIFLKELVD
                     VLDKCELYEEYDPWESYQFDARHLKGELREEEEAVMVVEF"
     gene            complement(8281..10098)
                     /locus_tag="PK154_01550"
     CDS             complement(8281..10098)
                     /locus_tag="PK154_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW09778.1"
                     /translation="MDKDYREESVRMAFRLFEEGKYLESLEICRAAGEQGTDTQIPIL
                     AARNLFNLDRMDEAEALVRDLLHGMPDSSYLHSFLGKILEQKHEDTAVAEYIRAITLD
                     PGNLEALRSYAACLMAQRDYRRAIPILKKISQLSGKEEDQRLLIQALTSAGQAREALV
                     VYRSSIRHTGADMDHVAALMGAGIFTEAAREALGAHRKTGSVECARLYLQALSRDHPG
                     SAEQEYREFFSALKDTGIGYDYAQLLLSAGKPADALQVVRDLLDDDRSGSHPRIRLLL
                     CRLNAMAGEKEKALGCFEYLIRETLSDIDDPGFLSDLLAKYREYLLTYYPVRDAVARF
                     LLQVSGTPHVVCLIESAKLYEDIGDHAEAQSYYYRAYRSDYLSGGIAYARYLARQAER
                     REGEKVLLYILNNVRKTRDLELLAGFILDEEWRFFSQQRILDRLVSVLEGHIPTLGSD
                     GFEYLSVAYLLSASESLKSRDYQSCKEYCLRGLDIVLSGSSHIRPEDFLELIKTCKEQ
                     TLSDTPALAPRRADPVKDERDEALRNFIESCDEQERVIIEFLRTHREASEMDLRQLLN
                     TRRVVGIMNRIMQKGALSGFPIIVKKGSGKGGEIYAYTG"
     gene            complement(10091..10792)
                     /locus_tag="PK154_01555"
     CDS             complement(10091..10792)
                     /locus_tag="PK154_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09779.1"
                     /translation="MKPDFSIIIPAYNEEHRIGNLLSQIPRRGGHFLFICDGSDSTPA
                     IVMKCARSHPETDLRCLVYPHRLGKGGAVKEGFVNADTAYVGFMDADASTSVTQMVSL
                     FEELGDADCVVGSRWLPGSIVPERQGIARRVESRGFNLLIRLLFGLSLTDTQCGAKVF
                     KKSAIDAVIGEMAATGFEFDVELLWRLSMKGYTIKEYPITWQNKGGSRVRGMDSFRML
                     KNLVSIRFFGGKEDG"
     gene            complement(10814..11059)
                     /locus_tag="PK154_01560"
     CDS             complement(10814..11059)
                     /locus_tag="PK154_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09780.1"
                     /translation="MQCTFTDNEETATLLKDLVWLNAVIATELIQITENVSSILRQSP
                     PPESCIRDHNRLREEALRIAEKYHPGTGLPEHLTGHQ"
     gene            11135..11545
                     /locus_tag="PK154_01565"
     CDS             11135..11545
                     /locus_tag="PK154_01565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPW09781.1"
                     /translation="MHYIERIRSYGRDANPFFTLMGIELGAIGNGCATLRMPIRPDML
                     NGEDFLQGGLFTALADEAMVLAIYPLLLPEERIATISESTTFMSGAQRGILVGTGRVI
                     KKGRRVIFAEGDVTLEGSDRILSRSTATFMVTKV"
     gene            11667..13256
                     /locus_tag="PK154_01570"
     CDS             11667..13256
                     /locus_tag="PK154_01570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPW09782.1"
                     /translation="MKYIFVTGGVMSGLGKGITAASIGRILKNRGYTVTAVKIDPYLN
                     IDAGTMNPGQHGEVFVLSDGGEVDLDLGNYERFLDIDLSSLHNITTGKIYRSVIEKER
                     RGDYLGETVQIIPHITDEIKCCIRAAACEEIPGAGKADICLVEVGGTVGDIESMPFLE
                     AVRQMRGELPVEDTVLIHVTLIPEDSMGDLKTKPTQHSVKALRELGLYADIIVGRGHR
                     PIGSNTKRKISAFCDLPQQGVISAATVPDIYQVPTEMEKEGAAEVLSRHLHLESRGVD
                     SDWYSLVAKEYTSRIEVGIVSKYGIEDVYMSIKEALKHAGRACSTEVKISWLDAERYE
                     DRHLGEFDGILIPGGFGKRGMEGKIEAIRYARTEKIPLLGLCLGFQMSVVEYARHVLG
                     WENATSAELGEGRHVIAILPEQQGVTDLGGTMRLGNYPITIKESTLAMQIYGQPVIVE
                     RHRHRYEVNPIYIPELEKAGLVFSGSWKNRMEICEVPDHPFFLATQFHPEFRSRPTRP
                     SPPFIGFVKACSAVKSGEVNS"
     gene            13256..14173
                     /gene="guaA"
                     /locus_tag="PK154_01575"
     CDS             13256..14173
                     /gene="guaA"
                     /locus_tag="PK154_01575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW09783.1"
                     /translation="MVQADRFVREAVDEIAAEAGHHRVVIALSGGVDSSVCAALASRA
                     IGDRLIPIYVDTGLMRKGETDRIARMFGHLNLRIIHAEDEFISALAGITDPEKKRKVI
                     GERFIRVFEREARKTGALFLLQGTIYPDRIESEGGIKSHHNVGGMPVEIEFEKVIEPI
                     RDLYKDEVREVAGALGLPPEIQHRMPFPGPGLAVRIVGEVTREKLEVVREANWIVEEE
                     LVDRYLPWQCFAAILGLGTGVKGDNRVHGWIVSVRAVNSRDGMTADPIEIPYDVLGIV
                     ASRITSEIPSVARVVYDITPKPPATIEYE"
     gene            14207..15346
                     /locus_tag="PK154_01580"
     CDS             14207..15346
                     /locus_tag="PK154_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HPW09784.1"
                     /translation="MNEMDYRASEARYYMPAFSREIMIERGHLSKVWDADGKEYIDCV
                     AGIAVCSTGHCHPEVTRAICEQAARLIHCSNLYYVPGQAELARKLVEVTGMHKVFFSN
                     SGAEANDGAIKLARLATGRKNFVAFTHGFHGRTLGSLSVTHKPAIREPFLPLEPSCTF
                     VDYGDLHALEKAVDGTVAGVFVEPIQGEAGVIIPPDGFIAGIREICDRHGALMIADEV
                     QTGMGRTGKWLAMQHTRIEPDIVTLAKGIASGFPMGALLAREGLEFKRGEHGSTFAGG
                     PLGCAAARATIEVIETILPDVPRKGKRFSSLLSGHQPRSSGLMIGLHIGNDCPVVQKR
                     CAENGVLVNCAADGNLRLVPPLVITNDEIDQAAAVINAAIAGIGK"
     gene            15446..16558
                     /locus_tag="PK154_01585"
     CDS             15446..16558
                     /locus_tag="PK154_01585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW09785.1"
                     /translation="MAGRITTGKRDALLVRDCFRAGGYVFAKKAGDLARENGISRIAR
                     LASNENPYPPSPDAIRQGCDALREANRYPDECMEQLLQCLRETYGSYHFVSGVGMDGV
                     IETLIRILVNPGDGVAIATPTFSFYGLAARAQSAEIRPLQRDEDYSVDPGIFIDAAAD
                     AKISFLCSPNNPTGTVTPPEIIEEILTGIEGILFLDNAYIEFCDTDYLPLMNRHDNLV
                     IGRTMSKAYALAGARVGYAFVPAWIVPFYQRASTPFALNSASAQAAIGALKDREFMNR
                     YVTHVRTWRERVARECGFPVTPSGANFIMVDVAPWTGDTMVRELAMRGILVRSCASFP
                     GLPDHYIRVSIGDEWENELFLKEIQAIREANSGLSQ"
     gene            16571..17083
                     /locus_tag="PK154_01590"
     CDS             16571..17083
                     /locus_tag="PK154_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HPW09786.1"
                     /translation="MMCGITGTPGTGKSSIAGALAQRGSPVLHLSETISPYIIEVDQK
                     RDTRVIDEERWAREFSRFNGFVEGHLAHLLPCDRIIILRCRPDVLMDRLRRRGYPEEK
                     VRENALAEALDVILIETLESFSPEQVYELDTTDKSIAECSMIADQFARGQVKASFGAI
                     DWSDYIGQIE"
     gene            17080..17676
                     /locus_tag="PK154_01595"
     CDS             17080..17676
                     /locus_tag="PK154_01595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPW09787.1"
                     /translation="MTLDSLRPHLKWIYKPFVDIAIRIGITPNHFSIAAFFVALFAGI
                     AFYFGEVVLGTFFIMCNAAFDSMDGAVARAMNIQGRKGDFLDHVIDRYADIFIITGIF
                     AGGFAPWPIGVFALTGVLMVSYLGTQAQAVGVGRYYGGILGRADRLLLLIIAGILNII
                     YPAGVFGLPFLGWLLVIFGLIGHITAIQRFIFVWKELG"
BASE COUNT         4120 a         4658 c         4852 g         4268 t
ORIGIN      
        1 cgggtatgtt tgatggatta aaagacgtct ctgctgtagc tctgcatatt gggaggatgc
       61 aggtgggaat cgggtcccgt accggtgcag gcattctgga tctggccaac ctgaggatcg
      121 cgaatgcgcc tcctggatcg actggaagga tggtgattga ccggctcctt actgagcaaa
      181 ctatcaatcc agatcaggtg ctggggaatg gcaattcggt aaaaacagag gccgcagtca
      241 tatccgtggt aatcaacggg agggctgatg caggaatctg ctctgcagtg cttgcgagag
      301 aagccggatt ggcatttgtt ccgatcacct atgaatcaca tgttctggta gcccgtaatg
      361 agtcgattga ggatgagcga attcatagcc ttctgcagat actccaatct ccggaattca
      421 gagattctct cgattattgt tatgatacaa cattgaccgg aaaagtagag gagtgtaata
      481 ctggatcctg gcaggaagtt tcttttttgg ataattggat aagcgattct gggggaggat
      541 aatattgatc aataccttcc atttgcctga atagacatcc aatcgccttc cctaatcatt
      601 ctcctttcaa gataccaaaa tgcagaagga ttgaaatgtc ttcctgtccg ggataatttt
      661 ttttaaaatg aggaattttt gaatcagtgt ttctgtttct ccttctcctc atcggggatc
      721 tcttcgaagt aaatgacttc tgccccgaca tcctttgcga tctgctcgat ctcaaacttc
      781 ctgaaatggg tcagctcgat ctggacggtt cctccggtta ctgcaacaat ccggaacatc
      841 ggctgcttga ttccttcgcg gactttcatc cgttccctga catagatctt catcttcatt
      901 gcgattcacc tgccgtgttg gttaatcaga gcatctgtta taccaactgg tttacagtat
      961 attgtattaa ccattacaga tataaatcta tgggagactg catgaaacgg gaccggtttg
     1021 aagttgccaa taagcttcag ggagagtcac tggtaaggaa agggctttta aaagcatatg
     1081 aaggcaggag ccagctcagg atgatgcccg atctgaatgt agtgaagatc gggggtcatg
     1141 ggatcatcga ttatgggaaa gaggtgctcc tgccgcttct gaccgagatc ggggagcttt
     1201 cacgtgagaa ccagatcctt gtggtcacgg gcgggggagt gagagtgcgt catatccttg
     1261 acctcggcct tgacctcggg ttgccgaccg gtgtccttgc agagcttgcc gggaagatca
     1321 gtgagcagaa tgcgatcatg gtatcaatcc tgctctcgaa ataccacggg acgcggatcc
     1381 ataccgggga ccttctcgaa ctccccatgc tgatgaacct tggcgtcctc ccggtcatcc
     1441 atgggacgcc tccctatggt ttatatgagc agcccgccca catcggctcg attcccccaa
     1501 accgcaccga tacaggggca tttcttgccg cagaggtact tggcgcaaag aacctcatcc
     1561 tcgtgaagaa tgtggatggt ttgttcacag caaatccctt cactgatacc acagccgaga
     1621 tcatcccgga tatcactgca gcagagctcc tggcaatgga gatggaggac atggttcttg
     1681 agccgagagt ggtcgagctg atgatgaatg ccaaacatgt gcatgaagtc aagatcatca
     1741 acggccatga gccagggaat ctccgtcgcg ccattgcagg cgagcgaatc ggtacggtaa
     1801 tccgcgcagt atgagttaaa acaccgactg acaggagatc atgccccagg attccaaacc
     1861 cccactcagg tttggtatcg gtgagctggc aggcagcgtt ggtgatttcg ggaccatcct
     1921 tcccctctct ctcgcgcttg ccgccactgg tgccctcggg atagggccag tcctcctttt
     1981 tctgggcatc tggttcatca tcaccgggta ttattaccgg tacccggtcc ccgttgagcc
     2041 catgaaggcc attgccgtca ttgcggtcgc tgccgggatg tcgggagggg agatcgccgc
     2101 tgccgggtat attctcgggg ccatcttcct cctcatcggt ttcaccaatg ttcttgaaat
     2161 aattgaacgg tatatcccac tgcctgtagt ccggggtatc cagctggggc tggcccttat
     2221 cctactcaaa acagcagtgg tatacctcat tcctgatccc ggacatttct tactgggggc
     2281 aatgatcatc agtggaggat tcctggtggc ataccacttc cgtgttccgg atctctcatc
     2341 aattgcggtc atcatcgttg cgattgcggc cggtatcctt gcacagggat tccctccgat
     2401 caccacactt tccctctccc ttggtgcaat ccccaccgga tcagagataa ttgcgtcact
     2461 ccgggagctg gtcctccccc aggctatcct caccatcacc aatgccattc tcgcaacgtc
     2521 attgctcgca aaagacctgt tttcagctga tatccgccca aaaaagctct ccaaaacaat
     2581 tggtctcatg aatcttacat cgattccgtt cggggggatg ccaatgtgcc acggtgccgg
     2641 tgggatggcc ggccagtacc ggttcggggc aagaaccggt ggtgcaaaca tctatgccgg
     2701 aatcttcctt atcggggctg cccttctttt tgcaagcacc gcatgggtcg gtctgatcgc
     2761 ccctggtttc tatgctgccc tcctcctctt tgtggcgatg gaactgggga ggcatggatt
     2821 gaagacaaat tcttacctgg ttaccctccc cactgcgatc ttatcgttgt tatcttcaat
     2881 gacagttgcc tttattgctg gtatgtgcct cgcgtacggt atcccctcaa tcaggagatg
     2941 gtttgcagaa aaaagcaggt gataggtgtg ccgggataat cccattcata ttgaccatgg
     3001 acatgggatt ccggcatgac tcctggtgtt atcacagagc gatgtacagg gtcacacatg
     3061 ttcctgggat gcatgaccat gaaaagatac gaagaggaaa tatataaatc tattttattg
     3121 aaaagaagaa ttaatcaatt ttaattaatc ctctcgtgtc cagtgaaccg gtcatcagac
     3181 agggttgcac tgaaacggga ttctcctgaa ggaaaccagc cagtacgctc ctcgcagaat
     3241 gcatcgcatt caggaagata gggtttgata tcaaggaggg gggtattgtc gagcatgtcc
     3301 actccgcgga tgtgaagact ggtatctttg caggcgatca gggacacgat cgatatcccg
     3361 aggggattag gccggttggg agctctggtt gcaaagacac catgcgggcg ggtgtcaaga
     3421 aacgggatca ggtgaagtct gcagggccct gaacggtgaa aatggtagat gagaatgatc
     3481 cgcgaaaaac catccatatc taaaagcccg tcagcaaact ccgggaaaat ctccacggtt
     3541 ccttcaatct ccttgccgcc agttggctga ataggcatcc ccgccggctc ctgaaaaggg
     3601 gtatgaatga tgccaattgg tctgaacagg atgccctctt ccataacaat tcctctcttg
     3661 atcaaagatc gggctgggta cctataagaa ggatggtatg tcacctcttc acccatcagg
     3721 gcgggtgtgc agttattcag aaaatcccca caccttacgg atgctcatcg gtccggaaaa
     3781 ccgggatgat atactcattc tggcatcgga tcccagggat ccactcagca ctatgccggc
     3841 attcccccta cgggatgtaa ttgaatctct gaagtattca aaacctgtaa ttgtttgatt
     3901 cgtcaacagt tcattcagaa gaattaactg agcctgactg agatcagggc ataccacatg
     3961 aacaataact accctgcttt ctcaaaaaat aggataagaa aggaagatct ctaggccatc
     4021 accacgccgc tctcttttaa cgctgccggg acattgccat aaccgagtgg tggactgatg
     4081 acttcctggc cgtcagcggc gaggatatcc tggcctgcct gacttatcag gatcttgatg
     4141 aattcgagtc caaggtccgg gctccgtgca ttctttggaa cggtaacagc atagatgatc
     4201 ggtgtacccg tctccgttgc agtggtggtt ccgctgatcc gcttcacttt aacggttgca
     4261 tacgaatcag cgttctcctg ggcggagagg tccattccca ccgggagttc gacatacggc
     4321 agaccgttct ggatagctac gctgctgtat tcgatcgcat agtcagcctt cccctctttt
     4381 aagatggtga ccacatcagg accggatttt ccaatcacga gcttgccatc aggggtgggc
     4441 ttggtcacat caatggtata tttcccatca gagaacgtcc tcgtcatctt gctgtgagaa
     4501 gcaaggaggc tcgaaaatat cgtgtcaacg ccatagaccc gttccgcgag ctggaccgac
     4561 attacagcac ggtatccagc cgggtcggtg ttgggatcgc tgatcacgta ttttacatca
     4621 ggggtgttca gaatctcata ccagttgtca gaagtgatcg tatcgctgtg cttgctctgg
     4681 ttggtataca ccactaccat gtggttcttt gcaaacgtca cataataatc ggcgtaggtc
     4741 gggtacatca gggttgggat gaggtaggca tcggctgagg cgagcacatc ggccttcttg
     4801 ccctgttttg tgatcttctc gatcatcgtg acgctccctc ctccaaagag ctgggcatcg
     4861 accagcggga actggctctc gtatgccgtc tctattttgg caaacggtgc attgaggctt
     4921 cctgcattaa agacggtcat cggccgcaga gcggttgcct ctcctgtaca gacacagttg
     4981 aactttgcgc agtcaccgtt catcgcagag aagaggttct tcccgtactt atccttccca
     5041 taattctcga tatcagcctg tgtctgtggg gagatcagga agttgacgaa gttattcgcc
     5101 atctcgatct tttccggtgt cgtcttgtcg ttatagaccg tgatgacaga gtagacgttg
     5161 agcaggatgg cgccttcact cacgacaggc tccagatcga gatcgccctg gtaagcaagg
     5221 taggttccct cgtcggtcag ggtgtaagcc cctttttctg atgcgagctg gagagtctcc
     5281 cccatacctc ttcctgcttc gatataccag ttacccgact tctggacatc cttggtatag
     5341 ttgtattttg ccgaggacca tacagccttt tcagcagagt gcgtaccgga attgtcacca
     5401 cgggatacga acataacgcc aggtgtgttt gcctttccct tggtgaggat ggtcgtgaag
     5461 gcctgttctg gggtcatccc tttcacaccg gccggatcat tcgcaggtcc gacgatgata
     5521 aagtagttgt aggcaaagca ccgcctgttc aacccggctc cgtcctccat ataggtaacc
     5581 tcctgcgagg gagagtggac ggccaggata tcacagtcac ctttctttgc gacttcaatg
     5641 gctttcccgg ttccctgcga tgtgatcagg agttcggcgt tatactgctc ctcaaacttc
     5701 ggtttcagat agtcgaggag ccccgtatcg tagagactcg ttgtggttgc aaggagtact
     5761 ttagtttttg gcccgacggg agttggtgga actgtggtcg gaacagtggt cgcattgcca
     5821 gtctcactgg gggtaggggt cacggtagga gtgccactcc ccggctcggt cgttgtgcag
     5881 ccggcaaagg ttgcaaagcc aacaaccaag agagtaagga ttatgaaaat gctgatctgc
     5941 ttcttcaatg ccataggcgt tcgggttaac ttttcgattg ggaataaatg aatattgtta
     6001 tttcttaggc atcagttaac caattgtatt atcaactggt tataccagac aatccatcgc
     6061 ccttactact ccaatctctc ttatcaatca gaaaggagag agaacaggtt gaagaggagg
     6121 tctttctgtc atattatcgt aatcttctct cgtacactcg gattttggct aattgcatga
     6181 gaatcatcct tttctcctgc acaaaaagag gagacctgca cccgtcattc cagcaataca
     6241 gagagtcaca gcatcgatac cgagcccggg gtcaggtatt tttgtaaagg ttacattggt
     6301 ctttgcaaaa gagacagatc ctgagtgagg aaggccgaga ggtaagttca cgacgtatac
     6361 cgtataagtt ccgggaatca gcctgcctgc aaggtagttg gtatcccaga tatatcgcca
     6421 cgttccatcg gggttcacat atgccgaggt gaagtaccca tgacccgcgg ggagattcag
     6481 gttctccagg cagaccccgc gtttgtcaag acctggtccg gataccagca ggaataccgc
     6541 gatagtgccc tttgcatcag tgctaccgct cagcacaagc cgctccccaa ttgcagaaag
     6601 ttccggagat gctccgatgg taagatgatc tgcagaaccg ccctggataa ggagtgcgga
     6661 caccagtaca gccaaaaaca ccaggctcag tcttccctgc acagggaaat gtctcttgtc
     6721 gttcccggtt caagctttcg ctcagtgatg tgattcatgg gaagagtgtg ccagtatatt
     6781 gatgaggaat acgcgggcag cctgaaacag tagaaaacga gaatattctt gaacaacggt
     6841 gagagaccag gttctcaaaa aatgttttca gacccgtgaa aaaattcaga tgattgagaa
     6901 gagtgaacct gatatcgttg tgggtaaaaa ggtgtgataa aaccctcctg gctctccaga
     6961 cgggaaaaat ctcgtaagga ggacattttc atcatcgctc agaactcgac gaccatcacc
     7021 gcttcttctt cttcccgaag ctcccctttc agatgccgtg catcaaactg gtagctctcc
     7081 cagggatcat attcctcata cagttcgcat ttgtccagga catcgaccag ctctttgagg
     7141 aatatccggg ggatgacatc gatccgcccc ccgaatttgc ccgtaagcct gttgatcata
     7201 gccctgatga accggtgtga gactctgttc cgatcaacct cgccatacgc ttctgaataa
     7261 atgatgatta ccttagaggc tacctgctcg agtttctttg catcaaactt ggagagcgag
     7321 atctggggct ggcggggatt tctgaattca ccactatcag taacaccgat ccggtcagcg
     7381 agaggtggga ttgatcggat ccccctcggg ccttcaaaaa aggctggagt acccgtgaag
     7441 aagaagtaac agtagggcat ctcgccccgg tctagagcat cgatgatcaa ccggagggtg
     7501 gtatatccct tctcacgctg gttcctcggg agcgtctgcg tcgtctcaag ctcatcaatt
     7561 gcaaccgcaa gaccagcgta tcctgcattg acaataatcc tgacgattgc acggaggaaa
     7621 atgagcgcga aggtctcatc aacatccccc cttattcctg cctttgcctt gaattccctg
     7681 ccgatgttcg gctctgcgga gatccagccg atagccgact gcgcagtctg gaaatcgccg
     7741 gcattgtttg cccggtagta ggtccggaga gccgcagcaa gggacgaatt catctcggat
     7801 ataccggcaa gagcggtttc tatctccttg agtgtggcaa tctcgagact gtcttccgga
     7861 agcgggtgat cgctcacttt caggagacgt tcctcaatgg agaagagcca cgtgtcgatc
     7921 actgccttca tggcatgctc ttcttcccgc gaccggaggg tcgcgcagat ctgctggtac
     7981 atccctttca acttgtagag gggggatgct ggtgagatca ccacatgggc agtgacaaag
     8041 ttcttctccc gtgctatctc aagggctctc gcgacaagaa aggtcttgcc gctcccgtaa
     8101 tcaccgcgaa taaatttcag gtctcctccg ccatttaaca cataatcgag ctgggaggcg
     8161 ataaccccct cctcaacatc aatcccgacc gcaatccgtt caagaccgcc agccggcacc
     8221 gttccgcgcc ggagtgcatt gataatgttg acgctcccgg tctttctctt gtccccatgg
     8281 tcagccagta taggcatata tctcaccccc ttttcctgag cccttcttta caattatggg
     8341 aaacccggat aaagcacctt tctgcataat ccggttcatg attccaacca ccctccgggt
     8401 attcaggagc tggcggagat ccatttcgct cgcttcacgg tgggtcctga ggaactcgat
     8461 gatcaccctc tcctgttcat cgcatgattc aatgaagttc cggagtgcct catcccgttc
     8521 atcttttacg ggatctgccc ttcttggtgc aagggcaggg gtgtcggaga gcgtctgctc
     8581 tttgcaggtt ttgatcagct caagaaaatc ttccggtctt atatgagaag aaccggatag
     8641 gacgatgtcg aggcctctca ggcagtattc cttgcagctc tggtaatccc tgcttttcaa
     8701 cgactcactc gcagagagga ggtatgcaac cgaaaggtat tcgaatccat ccgaacccag
     8761 ggtcgggata tgtccctcaa gcacgctgac gagacggtca aggatcctct gttgcgagaa
     8821 aaacctccat tcctcatcga ggatgaatcc ggcgagcaat tcaagatccc tggttttccg
     8881 cacattgttc agaatgtaaa ggagaacctt ctcgccttcc ctccgttcag cctgccgggc
     8941 aaggtaacgg gcatacgcaa tgccgccgga caggtaatcg ctcctgtatg cccggtaata
     9001 atacgactgc gcttcagcat gatcgccgat atcttcatac agctttgcag actcgatgag
     9061 gcagaccaca tggggggtcc ctgacacctg cagaaggaat cgggcgactg catccctgac
     9121 cgggtaatac gtcaggagat attcgcggta tttggccaga aggtcggaca ggaatcctgg
     9181 atcatcgata tctgatagtg tctccctgat caggtactca aaacagccaa gggctttctc
     9241 tttctccccg gccatcgcgt tcagcctgca gagcaggagc cgaatgcgcg ggtgggaacc
     9301 tgatcggtca tcatcgagga gatccctcac cacctggagc gcatcagcag gcttccctgc
     9361 agagaggagg agttgagcat aatcgtatcc gattcccgta tctttcaggg cagagaagaa
     9421 ttcgcggtat tcctgctccg cagatccggg atggtcccgt gaaagagcct gcaggtacag
     9481 ccgggcacat tcaacagagc ctgtcttccg gtgtgccccg agtgcctcgc gggcggcttc
     9541 cgtaaaaata cccgccccca tgagggcagc gacatggtcc atgtcagcac cggtatgccg
     9601 gatggaagac cggtatacca caagtgcctc ccgtgcctgg ccggcagaag tgagtgcctg
     9661 tatcaacagg cgttggtctt cctcttttcc ggagagttgt gaaatctttt taaggatcgg
     9721 gatggctcgg cggtagtcgc gctgagccat gaggcaggca gcatagctcc tgagggcctc
     9781 caggttccca ggatcgagcg tgatagcccg gatgtattcg gcaaccgcgg tgtcttcgtg
     9841 tttttgttcc agtatctttc caagaaaact gtgcaggtaa gacgaatcag gcatcccgtg
     9901 gagaaggtcc cgcaccagtg cttctgcctc gtccatcctg tcgagattga acaggtttct
     9961 cgctgccaga atagggatct gggtgtcggt accctgctca ccggcagcac ggcagatctc
    10021 gagtgattcg agatattttc cctcttcaaa gagcctgaag gccatcctga cggactcctc
    10081 ccggtaatcc ttatccatct tctttacccc caaaaaaccg gatggagacc aggtttttga
    10141 gcatccggaa ggaatccatc cccctgaccc gcgatcctcc cttgttctgc caggtgatcg
    10201 gatactcttt tatcgtatac cccttcatcg agaggcgcca taacaattcc acatcgaatt
    10261 caaatccggt cgctgccatt tctcctatga ctgcatcgat ggctgatttt ttaaatactt
    10321 tcgccccgca ctgtgtatct gtcagggaga gaccaaagag cagccgtatc agcaggttga
    10381 acccccggct ctccactctc cgggcaattc cctgccgctc gggaacaata gaccccggga
    10441 gccagcgtga acccacaaca cagtcggcat cccccagttc ctcaaagagg gagaccatct
    10501 gtgtaaccga agtcgaggca tcggcatcca tgaagcccac atacgcagta tcagcgttga
    10561 caaacccctc tttcaccgcc ccccctttcc cgagccggtg gggatacacc aggcatcgaa
    10621 ggtctgtttc cggatgggat ctggcacatt tcatgacaat tgcaggggtg gaatcgctgc
    10681 catcgcagat gaaaagaaaa tggcctcctc tgcggggtat ctgtgagagg aggttcccta
    10741 tccggtgctc ttcattatac gctgggatga tgatgctgaa atccggtttc aatcagaatc
    10801 gccgttgtct gttctattgg tgtccggtga ggtgctcggg aagtccggtc ccggggtgat
    10861 acttctcagc gatcctgagc gcctcttccc gcagcctgtt atgatccctt atgcaggact
    10921 ccggcggagg ggactggcga aggattgatg agacattctc ggttatctgg atcagttccg
    10981 tggcaatcac tgcattgagc cagaccagat ccttcagcag tgtggctgtc tcctcattat
    11041 ccgtgaaggt acattggatg cctgacggtt aaatgatgac ggcgattctg caggcaacca
    11101 ttatcggttt tcaaaaaaaa aacgtttttc atccatgcac tatatcgaga gaatccggtc
    11161 ttatggaaga gatgccaatc ccttttttac cctcatgggg attgagctgg gagcgatcgg
    11221 caacggctgc gccaccctcc gcatgccgat acgacctgac atgttgaacg gggaggattt
    11281 tttgcaggga ggcctcttca cagccctcgc cgatgaagcg atggtccttg ccatctatcc
    11341 tctcctgctc ccggaagagc ggattgccac catttctgag agcacgacgt tcatgagcgg
    11401 ggcgcagcgc ggaatccttg tcgggacggg cagagtgatc aagaaaggcc ggagggtcat
    11461 atttgcagag ggggatgtca ccctcgaggg atctgaccgg atactctccc gcagcacagc
    11521 cacgttcatg gtcacgaagg tctgacatgc gcttttcccc agctactctt cagaagaaaa
    11581 aaggaatcaa tggtgtgggg aataaaaata tctcccgatc gggagtatat ttaagacaga
    11641 agagtgcatc cggcgggtag ttttctttga agtatatttt tgttacaggg ggggtgatga
    11701 gtggacttgg caaagggatc accgctgcat ccatcgggcg tatcctcaag aaccgggggt
    11761 acacggtcac cgcggtgaag atcgatccct acctcaacat tgacgcgggg accatgaatc
    11821 cagggcagca tggagaagtt ttcgtactct ccgatggcgg tgaagtcgac ctggatctcg
    11881 ggaattacga gcggtttctt gatatcgatc tctcatccct gcacaacatc accacgggaa
    11941 agatttaccg gagtgtcatt gaaaaagagc ggagaggtga ctaccttggt gagacggtcc
    12001 agatcatccc ccacatcacc gatgagatca aatgttgcat acgagcggct gcctgtgagg
    12061 agattcccgg ggccgggaag gcggacattt gtctggtcga ggtgggaggg accgttggcg
    12121 atatcgagag catgccgttt ctggaagcgg ttcgccagat gaggggagag ctccctgttg
    12181 aagacacggt ccttatccat gtcacgctga tccctgaaga cagcatgggg gatctcaaga
    12241 caaaaccgac ccagcactcg gtgaaagcac tccgtgagct gggcttgtat gcggatatta
    12301 ttgtggggag gggccaccgc ccgattgggt ccaataccaa gaggaagata tcagccttct
    12361 gcgaccttcc ccagcagggc gtgatcagcg ctgccacggt ccctgacatc taccaggtcc
    12421 cgaccgagat ggagaaggag ggtgcagcag aggtactctc ccgtcacctg catcttgaat
    12481 cgcgcggagt ggattcagac tggtattcac tggtcgcgaa agaatatacc agcaggatag
    12541 aagtgggcat cgtgagcaaa tacgggatcg aggatgtcta catgagcatc aaggaagccc
    12601 tcaagcatgc agggcgtgcc tgttcgactg aggtgaagat ctcctggctc gatgccgagc
    12661 ggtacgagga tcggcacctg ggtgaatttg atggcatcct gatacccgga ggattcggga
    12721 aacgggggat ggaggggaag atcgaagcca tccggtatgc ccgcaccgaa aaaattcccc
    12781 tgcttggcct ctgccttggg ttccagatgt cagtcgtcga gtatgcgaga catgtgctcg
    12841 gatgggagaa tgcgacgagt gctgaactgg gggaagggag gcacgtgatc gcaatcctgc
    12901 ctgaacagca gggggtcacc gacctcggtg gaacgatgcg tcttggcaat tatccgatca
    12961 ccatcaagga aagcaccctt gcgatgcaga tctacgggca gccggtgatt gtagagcggc
    13021 atcgccaccg gtatgaagtg aacccgatct atatccccga actcgaaaaa gccggtctgg
    13081 tattctcggg gtcatggaag aaccgcatgg agatctgcga ggttcccgat cacccgttct
    13141 tccttgctac acagtttcac ccagagttca ggtcgcgacc gacgagacct tcacccccct
    13201 ttatcgggtt tgtgaaggca tgcagcgccg taaagtcggg cgaggtgaac agctgatggt
    13261 acaggcagat cggtttgtca gggaggcggt tgacgagatt gcagccgagg cagggcacca
    13321 ccgggtcgtc atcgctcttt cgggaggggt cgacagctcg gtctgcgctg cccttgcttc
    13381 gcgtgccatc ggtgacaggc tgatcccaat ctatgtcgat acgggattga tgaggaaagg
    13441 agagaccgac cggattgccc ggatgttcgg tcacctaaat ctccgtatca tccatgccga
    13501 agatgagttc atctcggccc ttgccggtat cactgatcct gaaaaaaaga ggaaagtgat
    13561 cggggagcgg ttcatcaggg tctttgaacg ggaagcgaga aagaccgggg ccctcttcct
    13621 cctgcaggga acgatctacc ctgacaggat cgagagcgag ggaggtataa aaagtcacca
    13681 caatgtcggg ggaatgccgg ttgagatcga gttcgagaag gtgatcgagc ccatccgtga
    13741 cctgtacaag gatgaagtga gggaggttgc cggggccctt ggtcttcccc cggagatcca
    13801 gcatcggatg ccgttcccag gcccgggtct cgccgttcgt atcgtcggcg aagtgacaag
    13861 ggagaaactt gaggtagtac gggaagcaaa ctggatcgtt gaggaggaac tggtggaccg
    13921 gtatctcccc tggcagtgtt ttgcggcgat actcgggctt ggtaccggtg tgaagggtga
    13981 taaccgggtt cacggatgga tcgtctctgt ccgggcagtg aattcgaggg acgggatgac
    14041 cgcggacccg atcgagatcc cgtacgatgt acttggtatc gtcgcatccc gcatcacttc
    14101 agagattccg agcgtggcaa gagtggtgta tgacatcacc ccgaaaccac ctgctaccat
    14161 agagtatgaa tagggaatat acccaaagat gtaaggcaga acatacatga acgaaatgga
    14221 ttaccgtgct tcagaggcac ggtactatat gcccgccttt tcacgggaga tcatgattga
    14281 gcgtggtcac ctttcgaagg tgtgggacgc ggatgggaag gagtatattg actgcgtggc
    14341 aggaatcgcc gtatgcagca cgggccattg ccatccggaa gtaacccggg ctatctgtga
    14401 acaggccgcg agactgatcc actgctccaa cctttactat gttcccgggc aggctgagct
    14461 tgcccggaaa ctggtggagg ttaccggaat gcacaaggta ttcttctcca actcaggtgc
    14521 cgaggcaaat gatggagcga tcaagcttgc ccgccttgca accgggagga agaatttcgt
    14581 tgcatttacc catgggttcc atggacgcac ccttggttcc ctctctgtca cgcataagcc
    14641 tgctatccgg gagccgttcc ttccgctcga accgtcctgc acgttcgtgg actatggcga
    14701 cctccacgcg ctcgaaaagg ccgtagacgg gacagttgcc ggggtctttg tcgaaccgat
    14761 ccagggagaa gccggggtga tcatcccccc ggatggcttc attgctggta tcagggagat
    14821 ctgcgatcgg cacggagccc tcatgatcgc tgacgaagtc cagaccggta tgggaaggac
    14881 gggcaaatgg ctggccatgc agcacaccag gatagaacct gatatcgtca cccttgcaaa
    14941 ggggatcgca agcgggttcc cgatgggggc cctcctcgca cgggaaggcc tcgagtttaa
    15001 gcgaggcgag cacgggagca cttttgccgg gggtccactg ggttgtgctg ctgccagggc
    15061 cacgatagag gtgatcgaga cgatactccc cgatgttccg cgaaagggga agcggttttc
    15121 cagcctgctc tccggccatc agccacggtc cagtgggctg atgattgggc tgcatatcgg
    15181 aaatgactgt cctgttgtgc agaagaggtg tgccgagaac ggggtgcttg tcaactgcgc
    15241 ggcagatggc aacctccggc tcgtaccccc gcttgttatt actaacgacg agattgatca
    15301 ggctgcagca gtgataaatg cggccatagc cggtatcggg aagtaacagc ggggatccct
    15361 ccccgcaatc ctttctctca gtctggcagg caccgcagtt tatcttcttt caggagttac
    15421 ctgttatacg actggtgcat caggaatggc aggcaggata accacgggaa agcgggatgc
    15481 gcttctggta agggactgtt tccgtgcagg aggctacgtc tttgccaaaa aagcgggaga
    15541 ccttgcacgg gaaaacggga tcagccgtat tgcccggctt gcaagcaatg aaaatccgta
    15601 tcctccctct ccggatgcaa tccgtcaagg ttgtgacgca ctcagggaag caaacaggta
    15661 tcctgatgaa tgcatggaac agcttctgca gtgcctccgg gagacctacg gatcttacca
    15721 ctttgtgagc ggagtgggaa tggacggcgt aatagagacc ctgatacgga tccttgtgaa
    15781 tcccggtgac ggggtggcga ttgccacccc gacgttctct ttttacgggc tggcagcacg
    15841 ggcccagtct gccgagatac gccccctcca gagagatgag gattattctg ttgacccagg
    15901 gatcttcatc gatgcagcag cagacgcgaa gatctctttt ctctgctcgc cgaataatcc
    15961 taccgggacg gtcaccccac ccgagatcat cgaggagatc ctcacaggga ttgaaggcat
    16021 cctctttctc gataatgcct atatcgagtt ctgcgatacc gactacctgc cgctgatgaa
    16081 caggcatgac aaccttgtga tcgggcggac catgtccaag gcctatgcgc tggcaggtgc
    16141 ccgtgtcgga tacgcctttg tacccgcctg gatcgtccct ttttaccagc gtgcctctac
    16201 cccctttgcc ctcaattcgg cctctgccca ggcagccatc ggagccctca aggacaggga
    16261 attcatgaac cggtacgtga cgcacgtcag gacgtggaga gagagagtgg cccgggaatg
    16321 tggctttccc gttacccctt cgggcgcaaa cttcatcatg gttgatgtag ctccctggac
    16381 aggagataca atggtcaggg agctggccat gcgggggatt ctggtcaggt cctgtgcaag
    16441 ttttccagga cttccggacc actatatcag ggtaagtata ggggatgaat gggagaacga
    16501 actctttctg aaggagatac aagcgatacg ggaggcgaat tccggtcttt cccaataaac
    16561 cggacctgct atgatgtgcg gaatcacagg gacccccggt accgggaaga gttcaatcgc
    16621 cggggcactt gcgcagcggg gttctcctgt cctgcacctc tctgaaacca tatcccccta
    16681 tataatcgag gtggaccaga agcgtgacac ccgggttatc gatgaagagc ggtgggcccg
    16741 ggaattctcc cgcttcaatg gatttgtgga aggtcacctc gcccatctcc tgccctgcga
    16801 ccggattatt atcctgcggt gcaggccgga tgtccttatg gaccggttaa ggagaagggg
    16861 gtatccggaa gagaaggtcc gtgagaatgc ccttgcagaa gcgctcgatg tcatccttat
    16921 cgagaccctg gaatccttct ctccggaaca ggtttatgaa ctcgatacaa cagacaagag
    16981 tatcgcagaa tgctcgatga tcgcagacca gtttgcccgg ggacaggtaa aggcctcgtt
    17041 cggtgccatt gactggtcag attatatcgg acagatagaa tgacccttga ttcgctcaga
    17101 ccccacttga aatggattta taagcccttc gtcgatatcg caatcagaat tgggataacc
    17161 ccgaatcatt tctccatcgc tgcatttttt gttgctcttt ttgccggcat tgctttttat
    17221 tttggggaag tggtgctcgg cacctttttc atcatgtgca atgccgcatt tgactcaatg
    17281 gacggagcag tggcacgggc gatgaacatc cagggcagga aaggggactt tcttgaccat
    17341 gtgatcgacc gttatgctga catcttcatc atcactggga tctttgccgg cggctttgcc
    17401 ccctggccga tcggggtatt cgccctgaca ggggtgttga tggtctccta tcttggaacc
    17461 caggcgcagg cagtaggagt tggccggtac tatggcggaa tacttggcag ggctgaccga
    17521 ctcctccttc tcatcatcgc cggcatcctg aacatcatct accctgccgg agtcttcgga
    17581 cttcccttcc tcgggtggct ccttgtgatc tttggcctca ttggccacat caccgccatc
    17641 cagaggttca tctttgtctg gaaagagctt gggtaagttc accggcaaag gcaggatagg
    17701 tggatgcagc ccacgatctc tctcacaata gaccccccct taagaaatga aaaaaataag
    17761 ggtatagtac tatttttacc accaaccctg tgattaaagt acgtctaata atattccatt
    17821 tttgcagttc caaaaatcaa tgaaaaaaaa gttaaatccc atatagcaat tcacaatgaa
    17881 ggaataatgt ggttgata
//
LOCUS       DAOOCO010000013        17255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2951_length_17255_cov_4.838488, whole genome shotgun sequence.
ACCESSION   DAOOCO010000013 DAOOCO010000000
VERSION     DAOOCO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17255
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_17255_cov_4.838488"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1284
                     /gene="malQ"
                     /locus_tag="PK154_01600"
                     /pseudo
     CDS             106..1284
                     /gene="malQ"
                     /locus_tag="PK154_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            1466..3640
                     /gene="glgP"
                     /locus_tag="PK154_01605"
     CDS             1466..3640
                     /gene="glgP"
                     /locus_tag="PK154_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPW09788.1"
                     /translation="MQDELIHRQGGSPIPDRIRGLADLSYNLWWSWHPEARVLFKQVN
                     PHAWRESIHNPVKMLRDIPDQFLNRVVGNPEYLRRYDIIMDRFSRYMENHNGWFSETH
                     PSSRTLTIAYFSAEYGLHHSLPFYAGGLGFLAGDHLKECSDLGVPLVAVGFMYSQGYL
                     HQRILPDGWQDNIREFLDREAAPVRRVTDSAGRQLLLDVPGITPSIKVAVWRVDVGKV
                     PLYLLDTDIPENTPQARAISSRLYTGDKEQRLVQEIVLGIGGRKVLSELGIDFSAVHL
                     NEGHPSFALLERTREFIEDGLSFPDALARVRGTSVFTTHTPVAAGHDVFPAPLIDKYF
                     SGYYPALGIDRDTFLDLGRNPGDPEGMFNMAAFALKFTAYHNAVSRNHGIVSRRMWHH
                     LWPDLVPEKVPIDVITNGVHLPTWMNQRMEGLFSKYIGPVYPHWQQEHDNPSLWEMID
                     EIPDKELWSLHVWLKAKLFNRIRERKRQRWASHLKEPLNLVADGLMLNPTVLTIGFAR
                     RFSSYKRADLIFHDPDRLKRIVTNRWLPVQIIFAGKAHPDDTEGKQILQRIYRYALTP
                     EFAGRIAFVEDYGEQTAQYLVHGVDVWLNNPLPPMEACGTSGMKAALNGVLNCSIRDG
                     WWLEGYNGKNGWAFGGEEWSPERDAMDAAELYTLLEKEIIPLYYNTSLDGIPHGWVKM
                     MKESIRSIAPVFSSRRMVKEYVRRYYPSLLKCAETGFETCPV"
     gene            complement(3896..4618)
                     /locus_tag="PK154_01610"
     CDS             complement(3896..4618)
                     /locus_tag="PK154_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW09789.1"
                     /translation="MVDKKDSLSFLMIILTILIIIQSGVVALLVLEVLLPAKDREAAA
                     DEVFSFSGSAIRFGQDGGYWGFIADDGRWYLPLNLPDEVMLSEGQRVEVTAIPGDLSL
                     IRTLVIPIRVIQLITPVAEHFSLIDTRWILETMQGNESVQSIPAGVRITLIFRENGTF
                     SGVAPANQYTGIYTHTCQVISFSGIEPTSRVGSHAWYDIEEQYFSSLRDTRLFEIRDE
                     FLHLMDEHQRTLLTFRADGVFT"
     gene            complement(4945..6159)
                     /locus_tag="PK154_01615"
     CDS             complement(4945..6159)
                     /locus_tag="PK154_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW09790.1"
                     /translation="MTPAGTLNRSAALRLILILGIVSLFGDIIYEGSRSVTGPYLLLL
                     GASALTVAFVAGFGEFIGYAARLVSGYLSDRTGNYWTYTIAGYLMIGAIPLLVFVQAW
                     EAAAVLILIERLGKGIRSPAKDTILSHAAHQVGRGWGFGIHEAFDQVGAIIGPLVFAV
                     SLALFGGYETGFAILALPFALLVVALMYARLRSPDPAEYENERVPVQAPPHSSRILLR
                     YGTFTFLTMAGFAVFPLMAYHFSAAAVVPTAQIPIFYAIAMAADALCALVIGKYYDRS
                     GITVLVAVPLASIPIAALAFAGGYYHALAAAVLWGISMGGQETVLRAALADLTSIKKR
                     GWAYGVFNTLYGGAWFAGSIVLGILYELDIRILVGYTVVMQAAALVAFFRLWRAVKDI
                     WKSPDRMLEDLS"
     gene            6286..6735
                     /locus_tag="PK154_01620"
     CDS             6286..6735
                     /locus_tag="PK154_01620"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW09791.1"
                     /translation="MAVVKENSAVMVSTLMEYFNKQPRIGTISTCSKDGKVDSAVFGS
                     PHMTDEKTVILGSGGNRTLANLSENPHAVYLIMEPGKSIMDWKGIRVYLRVKEIASSG
                     PLLETFKKNMAKVAGDQAAAMIHAVVSFEINEVRPLIDMGQGWENSI"
     gene            complement(6823..7167)
                     /locus_tag="PK154_01625"
     CDS             complement(6823..7167)
                     /locus_tag="PK154_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HPW09792.1"
                     /translation="MPEGPCFNQVWSEVLLKGVTPRRAYNRVKKECTEVYRIEPGYVF
                     REVPILLPHPMLVGINERESRILMPFRKPCFGLSLYAIDADHEEIVRLRTDFRKRTEY
                     SSEEDEGRTHKE"
     gene            complement(7398..7640)
                     /locus_tag="PK154_01630"
     CDS             complement(7398..7640)
                     /locus_tag="PK154_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09793.1"
                     /translation="MLIASVLFGLYHIGHSPPFNQAGMILLLSFIGIVTSLVYFVGWD
                     IYATIAFHNFLGCIGVIQALDQPDPFHSMKTPCSPF"
     gene            complement(7839..8126)
                     /locus_tag="PK154_01635"
     CDS             complement(7839..8126)
                     /locus_tag="PK154_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09794.1"
                     /translation="MSETLQRERDSDSCRILVLASLTYLIWVIVTYLLEGRILTLLRP
                     EAVIDRTAYTLIANILIGIILALMVIRKAERTRLISLETPGFRPRSVQLSR"
     gene            complement(8225..10639)
                     /locus_tag="PK154_01640"
     CDS             complement(8225..10639)
                     /locus_tag="PK154_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HPW09795.1"
                     /translation="MKLSATIALGFILGSLFFGSILSGITAADCACSAPSGLISDNAS
                     EGMAVPNAIAYENHTISFRNNCSESVWIAANMGPNGPPFILNNVSQGRGCTGCSCSGA
                     ATDGWKDNLTCCPSINCTHQSCPNSSCSCGVDLPNGGGFEVPPGEPVNYTVKADPQYG
                     GNAALFPRYGCSLSPDGVYLQCDTGTCYVQGTPGTPPEGIVRCGGVPPNGPPDQSPQL
                     PTTKMEIFFAPTNWSQTDSDYYDVSLADGYNIPVQIEPVQGTYWTGGTAHNWCIRAGG
                     TTDLLEKIQQPEYRNLSATMLVLRNNRPVALWSSCSYETQVNKTGPGQANATTTPGEG
                     EPIWNLSCCKGACGTEKTCPIDTLPENHRTSQFFGTFYDHTYTYQYGDDQANIPCKGE
                     KSTKQLTSYLITFCGGRILGSAYTHLVNVSPGIPATVHESTIGIDLTINGAEKRVHQR
                     VTILTFEPDHPPRNFTHPLTPVGPYYEISSNIPDAEITGMEIRAHYQDTGTTIEKDSL
                     RLFTYNGTWNPLSPGGIDNQNQTVWGLTNHTGVFAIAEYKPPKISDIGYTLPDEGDPL
                     TIRLTATVLGADPHTYAWDVNSDGIDDYSAADPVHTFDQPGEYQVRLKVTDGHGFSME
                     QLATVVVSEYAIPLAPGWNFISIPRRLDPNRTSAGIAFKPVETTTRAILTYNASTAMW
                     ENSGAESIVIPQTGFWLNSQQATTLPLIFDKNPANVPPAIHLARGWNAIGLGDSEPLA
                     ASLALKAVEDTWTVLIPFNRTTQGYDTSILRGSDGIHSDARQMEPGIGYWLFMTKEGE
                     LPATGA"
     gene            complement(10636..11154)
                     /locus_tag="PK154_01645"
     CDS             complement(10636..11154)
                     /locus_tag="PK154_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09796.1"
                     /translation="MDIVAALWPLFLPLIAFIIALLHIRWKQCNGYRAIGAVLMWQLV
                     IGLGLGYLWAGLGHILTPDSIAGSIGWPAGSPFQREVGMWDLSLGIVGILCLFFRDEG
                     FFTATIIGTGIFSISAGIGHLYELIFHGNTAVNNAGAVMYMDLFYPLVLAALLILYHM
                     KKRESRMKEGFI"
     gene            complement(11528..12772)
                     /gene="hisD"
                     /locus_tag="PK154_01650"
     CDS             complement(11528..12772)
                     /gene="hisD"
                     /locus_tag="PK154_01650"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW09797.1"
                     /translation="MWKAVEVETWIAGRKSSLEEARVTVTEIIERVRRDGDTALRQLS
                     RHVELTEIAVSDEEREAAYDVVDEKIITSLADAKDRIGHFHELQRSRDLWLEEVEPGI
                     VLGMKTTPLHRVGLYIPGGRAAYPSSALMCAIPARVAGVPEICACSPPPISPMTLVAL
                     DLAGVTEIYRSGGAQAIAAMALGTETIPPVQKIVGPGNIYVTLAKMMLREHVEIDFPA
                     GPSEIGIIADETANPRIVAADVLAQAEHDPNAACILITTDPDLPEKVGREVKAMTVKA
                     PRREIIEQALNNSGYVVVHDLEEAISASDAVAPEHLSIQVADPLPVVMRVQNAGAIFV
                     GRYSAVACGDYAVGTNHVLPTAGYAKTYSGLDVQHFCKTTSVEMISREGLEAIGDTVE
                     TIADAEGLHAHAESVRVRRSGK"
     gene            13003..14382
                     /locus_tag="PK154_01655"
     CDS             13003..14382
                     /locus_tag="PK154_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09798.1"
                     /translation="MEQGSAIIGFFRRFFRILVSIVGIGVILPVIIAVIAGFPAGIAL
                     SFILSAFALQAAVPPVGVALGLPVWLILVILTCFAAGVVLGIYEVCITLGASSKRVSS
                     FIGKVEQKTAKYPIIRKYGPITCILIAWIPGIGLYGTPVIAWMLGWKRIPSVICTAAG
                     FLIASLFVLFFASRINELLHLAAIAGVIVYAVTVSLSMGLSLPVSLIRGSLQKPILLL
                     SLLIVNFILVPIAASLIVSYLFLPSGASTGLVLLSLSAGAPSLYSWLRHQGGDGALLA
                     PHLAILCLVSIIFIPLMLPFLFPVGWDYLWMAFFYLGILVLVPLLAALYFSPRHEAAA
                     SRWSPVIDKVSWIALGVTVITMLILFTTAFFEVPGTISLIAAVLFVLVGYLAGYAAGG
                     RDPGVRRIFAMVTAPRNLADALIVAFLVFREGPVMVMALVAGLIGLVPFMINWWSMRK
                     HRTSLPKLL"
     gene            14866..15369
                     /locus_tag="PK154_01660"
     CDS             14866..15369
                     /locus_tag="PK154_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09799.1"
                     /translation="MFHNPHHFGIFLPGDLAFLPIEVLLVTLFLHQLLEKRDREMRLE
                     KLNMVIGTFFSSMGTSLPAYFSDRDLQMGEIRKSLRISDSWSEEDFTRMSAELSAYSS
                     RGTLKDIDPSGLRNFLLEKEDFLMRLLENPLLIEHESFTDMLLPVFHLTGELTHRKNL
                     TGLPAND"
     gene            complement(15459..15605)
                     /locus_tag="PK154_01665"
     CDS             complement(15459..15605)
                     /locus_tag="PK154_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09800.1"
                     /translation="MIWLVLIAGVYPQSFYLKVSFELWRKVQGMITLASSSKGFVLKA
                     REKR"
     gene            complement(15774..16004)
                     /locus_tag="PK154_01670"
     CDS             complement(15774..16004)
                     /locus_tag="PK154_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09801.1"
                     /translation="MIEKSGYDLVASFSLPSEAWWNEYYTPLQIKLIGMEEEYAENQD
                     ALAVIDMTWREIEQFQKYSDYCGYQGFLPRKI"
     gene            complement(16251..16514)
                     /locus_tag="PK154_01675"
     CDS             complement(16251..16514)
                     /locus_tag="PK154_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09802.1"
                     /translation="MDTPFIFTLFEGLDRKGPGSDACTNRMFNIPSPLQILGFLISVA
                     VPTARRSPLPDSAGTATSRLLTATSLTVIRSGKGQMRKEPVGG"
     gene            16747..>17255
                     /locus_tag="PK154_01680"
     CDS             16747..>17255
                     /locus_tag="PK154_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPW09803.1"
                     /translation="MAAAPDPTSLKDLLSRLSRHPAFLGALIGILAAFSQSLLISAGG
                     PEAYGFCVACHTRDLVNGMTNIITGASLALAPISKNAILPVMSVVGVIIGAFLSAKVH
                     KEHKIKKTDYQEYLIYFIGGFVVLQLAMVFGGCPYRAALRTGYGDITALLFIIMMGLG
                     VIAGTLLMLR"
BASE COUNT         3954 a         4605 c         4568 g         4128 t
ORIGIN      
        1 cccgagataa tggtatccga tgggaaggct gatcaggagc tgttaaaacg cctccgttct
       61 ggtgaaaacc acgtcttggg ttctccccac ggggccagcc ggaagatgga atttttacgg
      121 gctgcagcgc ggcagaacga ttacgggaga gacgatccgg ggtttgcgca tttccggttg
      181 gaacatcgct gctggcttga tgattacgca ctcttctctg ccatttcaga aagcaggggt
      241 accacctggt cagactggcc agatccactc agggaccggg aacctgatgc cctcgaggaa
      301 gagcgcagga agctcggcac ttctcttgaa gaagagcagt acattcagta tcttgcattc
      361 tcgcaatgag atgctctcca tgcatttgca cggaatcatt gtgtcagtat tgtcggggac
      421 ctcccgctgt atcctgcgta tgaaagtgcc gatgtctggt cccataaaga attgttccgt
      481 cttgatgagg ggggaagacc ccttgcggtg gcaggggtgc cacccgatta cttcagcacc
      541 accggccagt ggtgggggaa tcctgtctat cggtgggaag agatggaatc tcaggggttc
      601 tcctggtggg tgcagcgggt cgggcacctt ctctcgatgg ttgactgtat caggatcgac
      661 catttccggg gactctctgc ctgctgggag atccctgcca gtgctgtgac tgccggtgag
      721 ggtcactggg tcccggcacc cgggtacgcc ctcctctcct gcctggcagc ccggttccct
      781 tcacttccct tcgtcgccga ggatcttggt acgatcacgc cagacgtgag gaacctgatg
      841 accgccttcg gaatccccgg gatgcgggtc ctccagtttg gattcaaccg cgatcccaac
      901 aatccgcatg ccccggaggc catcaaggag gacgtcgtgc tctataccgg gacccatgac
      961 aacaataccg tgagagggtg gtttgaagag gaggcttcgc tggaagagcg tgaacggata
     1021 acatcggtcc tcggaaagct tccggaaaca gggacgattt cacacgactc aatcgattgt
     1081 gccttccaca caaaggcacc tgtcgtgatc atccccgtgc aggatctcct cggtcttgga
     1141 agcggagcac ggatgaaccg gccggcaacc accgaaggga actggcagtg gagattgcag
     1201 ccgggggaac cccgtgcaga ggactgggca tggctgcggg agctgacaat ccggtcgggg
     1261 cggcattctc ctgcagcgga atagcgggaa gaggtgagaa aagaggaggg tgatggcagt
     1321 atcgcctcgt tatctgcagg caggagggcc gctcttttat catccccccc ataattcacc
     1381 gggatcttcc tgccagaata catcctcatg gggccgcatg agaaagatat acctcttttt
     1441 aaaactgata acgctatgac catttgtgca ggatgaactg atacatcggc aagggggatc
     1501 tcctatcccc gatcggatac gaggactggc tgatctctcc tacaacctct ggtggagctg
     1561 gcacccggaa gcccgcgtcc tcttcaagca ggtcaatccg catgcttggc gggagagcat
     1621 ccataatccg gtgaagatgc tcagggacat acccgaccag ttcctgaacc gggttgtcgg
     1681 aaatcccgag tatcttcgcc gctacgatat catcatggac cgtttctccc ggtacatgga
     1741 gaaccataat ggatggttct ctgagaccca cccctcctcc cgaacgctca ccatcgccta
     1801 tttttccgct gagtacggac tgcatcattc tcttcctttc tatgcagggg gtctgggatt
     1861 cctcgcaggg gaccacctca aggaatgcag tgatctcggg gtcccgctgg tagccgtggg
     1921 attcatgtac tcccagggct atctccacca gcgaatcctg cctgacgggt ggcaggacaa
     1981 tatccgggag tttcttgacc gcgaagctgc ccctgtaagg agggttaccg attccgcggg
     2041 aaggcagctc cttctcgatg tgcccggaat aaccccctcg attaaggtcg ccgtttggcg
     2101 ggtggatgtc ggcaaagtcc ccctgtacct ccttgatacc gatatccctg aaaatactcc
     2161 ccaggcccgg gcgatctctt cccggctcta caccggagac aaggagcagc gcctggtcca
     2221 ggagatcgtc ctcgggatcg gcggacggaa ggtgctttct gaacttggaa tcgatttttc
     2281 cgcggttcac ttaaacgaag ggcatccctc gtttgccctc ctggagcgaa cacgagagtt
     2341 tattgaggac ggactctcct tcccggatgc cctggcccgc gtcaggggaa cctcggtctt
     2401 cacgactcat accccggttg ctgccggcca cgatgtcttc ccagccccgc tgatcgacaa
     2461 gtacttttca ggatactacc ctgctcttgg catcgacagg gatacctttc tcgaccttgg
     2521 caggaatccg ggcgatccgg aggggatgtt caatatggcg gcttttgcgc tcaaattcac
     2581 cgcgtaccac aatgccgtgt cccggaatca cggcatagtc tcccggagga tgtggcatca
     2641 cctgtggcca gatctcgtgc ctgagaaggt cccgatcgat gtgatcacga acggggtgca
     2701 tctcccgacc tggatgaacc aacggatgga agggctcttc tcgaaatata tcggtccggt
     2761 gtatcctcac tggcagcagg aacatgacaa tccctccctt tgggaaatga ttgacgagat
     2821 cccggataag gaactctgga gcctccacgt ctggctcaag gcaaagctgt tcaaccgcat
     2881 cagagagcgg aaacggcaaa gatgggcttc tcacctgaaa gaaccgctga accttgtcgc
     2941 tgatggcctg atgctcaatc caaccgtgct tacgatcggt tttgcacgcc gattttcgag
     3001 ctacaagcga gctgacctga tcttccatga tcccgaccgt ttgaaacgga tcgtgacgaa
     3061 ccggtggctg ccggtccaga tcatctttgc cgggaaggcc caccctgatg ataccgaggg
     3121 gaaacagatc ctgcagcgga tataccgata tgcgcttacc ccggaatttg cagggaggat
     3181 cgcctttgtc gaggactatg gtgagcagac cgcccagtac cttgtccatg gggtcgacgt
     3241 ctggctgaat aatcccctcc cccctatgga agcctgcggg accagtggca tgaaggcggc
     3301 cctgaacggg gttctgaact gcagcatccg tgacgggtgg tggctcgaag ggtataacgg
     3361 gaagaacggc tgggcatttg gcggtgaaga atggagccct gagcgggatg ccatggacgc
     3421 agcagaactc tataccctgc ttgaaaagga gatcatcccc ctttactaca acacctccct
     3481 ggatgggatc ccccacggat gggtgaagat gatgaaagag tcgatcagga gcatcgcacc
     3541 cgtgttttca tcccggcgga tggtcaagga gtacgtccgg cggtattacc cctccctcct
     3601 gaaatgtgca gagaccgggt ttgagacatg cccggtatga gagcacctat cagtagtatc
     3661 aaaaccagat acaggatttt gagcgtgaca gaaaggggat ctcccctcgc tggtgtgttt
     3721 gcctttgaag aaccagatat ttcttttgta tatgctgtcc ccctcaaacc ccaccaccaa
     3781 tgtacgctac cgatcaagaa gggcactcca agggcggctc cttggatcat gttctgatgc
     3841 caatcccccg gttcagcttt tcaaaacaac accgcaagaa ggcaaaaagg ttatttcagg
     3901 tgaatactcc gtcggccctg aaggtgagga gcgttctttg gtgttcatcc atgaggtgaa
     3961 ggaactcgtc ccggatctca aatagacggg tgtcgcgaag tgatgagaaa tactgttctt
     4021 cgatatcgta ccacgcatga ctgcccactc tgctggtcgg ttcaattccc gagaaggaga
     4081 tgacctggca ggtatgcgta tagatgcccg tatactggtt ggcaggtgct actccgctga
     4141 atgtcccatt ttcacggaaa atcagagtga ttcggactcc ggcgggtatg gattgcacgg
     4201 actcattccc ctgcatggtt tcgaggatcc atcgcgtatc gatcagagaa aagtgctcag
     4261 ctaccggggt gataagctgg atcaccctga ttggtatcac caatgtccgg atgagagaga
     4321 gatcacccgg gatcgctgtc acttctaccc gctgcccctc agagagcatc acctcatcgg
     4381 gaaggttgag tggcagatac catctcccat catcagcgat gaatccccaa taacccccgt
     4441 cctgcccgaa cctgatagca ctcccagaga atgaaaacac ctcatctgca gctgcctccc
     4501 ggtcctttgc agggaggaga acctccagaa cgagaagtgc tactaccccg gactggataa
     4561 taatcaggat ggtaaggatg atcatcagga atgagaggga gtccttctta tcaaccattg
     4621 cactctattg gtcatgattg actgataatt cctctccact ggttccgata tttgaatatc
     4681 cggcctgggg aatcagaggt atcgttaaag gaagtgtctt caggaactct tatggggagg
     4741 tgggtctcca gcacctcgct atctctggca taaaatatac cccattgatc cgggattgag
     4801 gggggccgca gtgaatatcc cgtgcttcca acctccctca ctcgattatt cagtctcccc
     4861 ttctatctcc tcccatggaa gtgttcgatc gctcagaagg tcatatccag ctggcagaag
     4921 actgttcctc ctcgtttata ccgatcagga tagatcctct agcatcctat ccggtgactt
     4981 ccatatatcc tttaccgccc tccaaagccg gaagaacgcc acgagtgcag cggcctgcat
     5041 gacaacagta tacccgacga ggatccggat gtccagctcg tacaggatcc cgagcacgat
     5101 gctccctgca aaccaggcgc ccccatagag ggtgttgaag accccatatg cccacccccg
     5161 tttcttgatc gaggtgagat cagcgagtgc tgcccggagc acggtctctt gtcctcccat
     5221 cgaaattccc cagaggactg ccgccgcaag cgcgtggtaa tatcccccgg caaaagcgag
     5281 agcagcaatc gggatgctgg ccaggggaac ggctacaagg acggtgatcc ctgaccggtc
     5341 atagtatttc ccgataacga gggcacacaa ggcgtcggct gccatggcta tggcgtagaa
     5401 tatcggtatc tgggcggttg ggaccacggc agcagcactg aaatggtagg ccatcagggg
     5461 gaataccgca aatcctgcca tcgtcaggaa ggtgaaggtg ccgtagcgga ggagaattcg
     5521 ggaagaatgc ggtggtgcct gaaccgggac cctttcgttt tcatactctg ccgggtcagg
     5581 agatctcaat cttgcataca tcagagcgac aaccaggagg gcaaacggga gtgcgaggat
     5641 ggcaaatccg gtttcatatc caccaaacaa cgcgagagaa accgcgaata ccagcggacc
     5701 gatgatcgcc cccacctggt cgaaggcttc atggatccca aatccccagc cccttccgac
     5761 ctggtgtgca gcatgggaga ggatggtgtc cttggctggt gatcggatcc cttttcccag
     5821 ccgttcgatc aggataagaa cggcagctgc ctcccatgcc tgcacaaaga caaggagggg
     5881 gatcgccccg atcatcagat acccggctat ggtatatgtc cagtaattgc cggttcgatc
     5941 ggaaaggtac ccggatacca gcctcgcagc gtagccgatg aactcaccaa atcctgccac
     6001 aaaggcgacc gtcagtgcag atgcaccgag caggagcagg tacggccctg tgacgctcct
     6061 gcttccttcg tagataatgt ccccgaagag actcacgatc ccaaggataa ggataaggcg
     6121 gagtgcggca gaacggttga gggttccagc aggggtcatc gggtagtcag ggatttactc
     6181 tgaagcacca tgtaatcgtt tcggaatgat accgggaagg tatatcctcc ctctttgaac
     6241 gcatgaagta tcccataaca tttatcgaac cagcaggata gacagatggc tgtagtgaag
     6301 gagaacagtg cagttatggt atcaacactg atggaatatt tcaacaagca gcccaggatc
     6361 ggaacgatca gtacctgcag taaggatggc aaggtcgact cagccgtatt cgggtccccg
     6421 cacatgactg atgaaaagac agtgattctg ggatcagggg gtaacaggac ccttgccaac
     6481 ctcagcgaga atccccatgc cgtttatctc atcatggagc cagggaagag catcatggac
     6541 tggaaaggga tacgggtcta cctgcgggtg aaagagatcg catcatccgg gccgttgctg
     6601 gagacgttca agaagaacat ggcaaaggtt gccggcgacc aggcagctgc catgatccac
     6661 gccgtggtga gcttcgagat aaatgaggtc cggcctctga tcgatatggg gcaagggtgg
     6721 gagaattcaa tttaaatcct tttccgtgta tcattcgccg gaaaggtatg gtgcagggga
     6781 ggagagagga ttatctggat gatccggtga tgagacccgg gcttattcct tgtgggtcct
     6841 cccctcatcc tcttctgatg aatactctgt tcttttccga aaatcggttc ggagcctgac
     6901 aatctcctca tggtcggcat cgatagcata cagcgacaaa ccaaaacagg gtttccggaa
     6961 cggcatgaga atccgcgatt ccctttcatt gattcctaca agcatgggat gtgggaggag
     7021 gatcggcact tcgcggaaca catacccggg ttctatccgg tagacctcag tgcattcctt
     7081 cttcacccgg ttgtatgccc ggcgtggtgt cacgcctttc agcagaacct cagaccagac
     7141 ctggttgaag cagggacctt ctggcattac tgaagggata tcccatcgga attaagtacc
     7201 tttccctgca cgaattgtcc tatgggaaag gttctcctcg atggcctcaa gaacgagctc
     7261 cgcttaaatc tcctacttta ggaaaccata gatctgaaca cgacagaatc cggttcatgt
     7321 tcaggcatgt tgagaacctt gcctgatgaa ccgtaagtca atggcgataa agactgcaac
     7381 tgacatagtc gccatcccta aaacggggag cagggggttt tcatagagtg gaagggatcc
     7441 ggctggtcga gtgcctggat aacgccaatg caaccaagga agttatggaa tgcgatggtg
     7501 gcatagatat cccacccgac gaaatagacg aggcttgtca ctattccgat gaaggaaaga
     7561 agcaggatca ttcctgcctg gttgaaggga gggctgtgcc cgatatggta cagcccaaaa
     7621 aggacacttg caatgagtat tccgcccgca atggcgatta tcctcccttt ttttaaaaag
     7681 agttccctct gtgatcgatc cgatcatgcc ccagcacatg acaacctctg caatggtcac
     7741 tatcagcacc tgggcatata cattgagaag cacaacaagg ttgggtgctc cgtgatgctg
     7801 gatcagcagt atggctagtc cacccagaaa tgcgatgatt accgcgataa ttgtacgctt
     7861 cgggggcgga atccgggtgt ttcgagggag atgagtcgtg ttctctcggc ctttctgatc
     7921 accataagag caagaatgat gccgatgagg atgtttgcaa tgagggtgta ggctgtccgg
     7981 tcgataacag cttcaggcct gagaagggtc aggatcctcc cctcaaggag ataggtgacg
     8041 atcacccaga tgagataggt gagggatgca aggacaagga tccggcagct atcggaatct
     8101 ctctctcgct gtaacgtttc actcattggt tgctgccttc cgatctcctg cgcgaaatac
     8161 caccatttac cacacccgga agttcttcat tcaaccattc tcatcggaat ttaaagataa
     8221 aatattatgc tccggtggcc ggcagctctc cctcttttgt catgaacagc cagtacccaa
     8281 tccccggctc catttgtcgt gcatcactgt gtatcccatc agatcctcgg aggatggagg
     8341 tatcgtaccc ctgtgtcgtt ctattgaagg gtatcagtac ggtccaggta tcctcaactg
     8401 ccttgagtgc cagggatgca gcgaggggct cactgtcacc aagaccaatt gcattccatc
     8461 cccgggcgag gtgaattgcg ggaggtacat ttgcagggtt cttatcgaat atgagaggaa
     8521 gagtggtggc ctgctgcgag ttgagccaga atccagtctg tggaatgaca atcgattccg
     8581 caccactatt ctcccacatc gcagtggaag cattgtaggt caggattgcc cgtgtggtgg
     8641 tctcgaccgg tttgaaagcg atcccggcag aagtgcgatt gggatcgagc cgcctgggta
     8701 ttgagataaa gttccagccg ggagcaaggg ggatggcata ttccgatacc acgaccgtgg
     8761 caagctgttc catggaaaat ccgtgtccat ctgtcacctt cagcctgacc tggtattctc
     8821 cgggttgatc aaaggtatga accgggtctg ccgcagaata atcatcgatc ccatcactat
     8881 tcacatccca tgcataggta tgaggatctg ctcccaaaac ggtggctgtg agcctgatgg
     8941 tgagcgggtc tccctcgtct ggcagggtat atccgatatc gcttatctta gggggcttat
     9001 actctgcaat cgcaaatacc cctgtatggt tggtcagccc ccagacggtt tggttttggt
     9061 tgtcaatgcc cccgggactg aggggattcc atgttccgtt gtacgtgaac aacctcagac
     9121 tgtccttctc tattgtagta ccggtatcct ggtagtgggc tctgatctcc atccctgtta
     9181 tctccgcatc agggatgttg gatgagatct cgtagtaagg gccaactgga gtgagtgggt
     9241 gagtgaagtt ccggggtggg tgatccggct cgaacgtcag gatggtcacc cgctggtgta
     9301 cccgtttttc ggcaccgttg atggtcagat cgatcccgat agtggattca tgtaccgttg
     9361 cggggatacc cgggctgaca ttcacgaggt gcgtatatgc tgatccgaga attcttcctc
     9421 cgcagaacgt gatcaggtaa ctggtcagct gcttcgtgga tttttctccc ttgcagggga
     9481 tattggcctg atcatcgcca tactggtagg tatatgtgtg gtcgtaaaag gtcccaaaga
     9541 actggcttgt ccggtgattt tcgggaaggg tgtctatcgg acatgttttc tcggttccgc
     9601 aggctccctt gcagcaggag aggttccaga tcggctctcc ctctccaggt gtcgtagtgg
     9661 catttgcctg gcctggcccc gtcttgttca cctgggtctc gtagctgcat gagctccaga
     9721 gggcgaccgg cctgttattc cgaagaacaa gcatagttgc gctcagattg cgatactccg
     9781 gctgctggat cttttcaaga aggtccgttg tgcctccggc tctgatacac cagttgtgtg
     9841 cagttccgcc tgtccaatag gtcccttgca ccggctcgat ctgcacagga atgttgtacc
     9901 cgtcggcaag actcacatcg taataatcgc tgtcggtctg gctccagttg gtgggggcaa
     9961 aaaaaatctc catctttgtt gtcggaagct ggggactctg gtcaggaggg ccgttgggcg
    10021 gcaccccgcc acacctgact attccctcgg gaggtgttcc gggggtgccc tggacgtaac
    10081 aggtaccggt atcacattgc aggtataccc catcaggaga aagactgcag ccataccttg
    10141 gaaagagtgc ggcattgccc ccgtactgcg gatcagcttt cacggtatag ttcaccggtt
    10201 ccccgggcgg aacttcaaat ccgccaccat tcggcagatc aactccgcag ctgcagctcg
    10261 agttcgggca ggactggtgc gtacagttga tcgagggaca acaggtcagg ttgtccttcc
    10321 agccgtccgt tgctgctccc gaacaactgc aaccggtgca gccccgcccc tgtgacacat
    10381 tattcaggat aaaggggggg ccattgggtc ccatgttcgc agcaatccat accgattctg
    10441 aacaattatt tctgaaagag atggtgtggt tttcatacgc aatagcgttg ggtactgcca
    10501 tcccctcaga ggcgttgtct gatatcaggc cactcggtgc gctgcatgcg caatctgccg
    10561 cggtaatacc actgagtata cttccaaaaa aaagacttcc caggataaac cccagggcga
    10621 tggttgcgga taatttcaaa tgaatccctc cttcatccgt gattctctct ttttcatgtg
    10681 atacaggatg agcagggcgg caaggacgag cgggtagaag agatccatgt acatcaccgc
    10741 acctgcattg ttcaccgcag tgttgccatg gaatataagt tcatacaggt ggccgatgcc
    10801 ggcagaaatt gagaatattc ctgtcccaat gatagttgcg gtaaagaatc cttcgtctcg
    10861 gaagaagagg cagaggattc caacgatccc aagcgagaga tcccacattc cgacttcccg
    10921 ctggaacggg ctgccagcag gccatccgat ggacccggca atgctgtcag gagtcaggat
    10981 gtgccccagg cctgcccaga ggtacccgag cccaagtccg atgacgagct gccacatcag
    11041 cactgcgccg attgcccgat acccgttgca ctgtttccag cggatgtgga ggagtgcaat
    11101 gatgaacgcg atgagaggaa gaaaaagggg ccagagagcc gccacgatgt ccatgaaggt
    11161 gatttctgcc aggatatgat aaatgagaat ggttttattc gattattctt cccttgacag
    11221 atgaatagta atccttgcct ggaggtgaat ccgggtaccc ttctctcctg caggaatgaa
    11281 tcctggagat tcatgggagg caaggtcatc ctgatcggta gaaggtatca ctcctgaaat
    11341 gtctggggtc catgatagac tgtatggttc agtaactccc attgtgccgt attttcccaa
    11401 cgatccctgg atttctttaa cacaagtgaa tgatttgtgc cccgatacgt gatgcacgga
    11461 ttttgtatca gaaaagagtc gggggaagtt ccctctttgg cagcagcccg cagatccctg
    11521 ggatgctcta tttcccgctt ctccggaccc tcacggactc tgcatgggca tgcaggcctt
    11581 cggcatcggc aatcgtctcg accgtgtcgc ctatcgcttc gagaccttca cgggagatca
    11641 tctctaccga ggtagtcttg cagaaatgct ggacatcgag ccctgaatat gtttttgcat
    11701 accctgcggt cgggagtaca tggttggtac cgacagcata atccccgcag gcgactgcgg
    11761 aataccgccc gacgaagata gccccggcat tctgaacacg catcacgacc ggcaggggat
    11821 cggcgacctg gatagagagg tgttcagggg cgacagcatc ggacgcagag atagcctctt
    11881 cgaggtcatg caccacaaca tagcctgaat tgttcagtgc ctgctcaatg atctctctcc
    11941 ggggtgcttt tacagtcatc gctttcacct cccggccaac cttttcaggg agatcaggat
    12001 ccgtggtgat gaggatacag gcagcattcg gatcatgctc agcctgggcg aggacatcgg
    12061 cagcgacgat tcgtggattg gcagtttcat ccgcaataat cccgatctcg ctcggcccgg
    12121 ctggaaagtc gatctcaaca tgctcccgga gcatcatctt tgcgagagtg acgtagatat
    12181 ttcccggtcc gacaatcttc tgcaccggtg gaatggtttc ggtaccaagt gccatcgctg
    12241 cgatggcctg tgctcctcct gaccggtaga tctcagtcac tcctgctagg tcaagggcaa
    12301 caagggtcat cgggctgatg gggggtggag agcaggcaca tatctcggga actcccgcaa
    12361 cccgggcagg tatggcgcac atcagtgcag acgaaggata agcagcccgg cctccaggga
    12421 tataaaggcc tacccggtgg agtggtgtcg ttttcatccc gagcacaata ccgggttcga
    12481 cctcttccag ccagagatcc ctggatcgct gcaactcatg gaagtgccct atccgatctt
    12541 ttgcatcggc aaggctggtg atgatcttct catccacaac atcatatgct gcttcccgtt
    12601 cttcgtcaga taccgcgatc tcagtgagtt caacgtggcg ggagagctgc cggagggctg
    12661 tatcaccatc cctcctgact ctctcaatga tctcggtcac ggttacccgg gcttcttcga
    12721 ggcttgactt ccgacctgct atccaggtct ccacctccac tgccttccac atagtgccaa
    12781 atacatcagt caggaatgat gttaatgttt cgaagcctgg aaccttggta ttcctgtcct
    12841 gtcggaaagc ttcatatcag gtcattggtt tctgtccagt tctctgaaca cacttcgagc
    12901 gaatgaactc caatgaaaag agactcggta atggcacctg tcgaatgatg ttcgattggg
    12961 gtgtctcaat aagattttat agatgatccc tgattcagat gtatggaaca aggcagcgct
    13021 atcataggct tcttccgaag attcttccgg attcttgtct ccattgtcgg gatcggggtc
    13081 attcttcctg tgatcattgc tgtcattgca ggattccctg ccgggattgc actgagcttt
    13141 attctctcgg cctttgccct gcaggcagca gtgcccccgg ttggggtggc gctgggactt
    13201 ccggtctggc tgatcctcgt aatccttacc tgttttgctg ccggggttgt actcgggatc
    13261 tacgaggtct gtatcacgct tggggccagt tcaaagaggg tatcctcctt catcgggaaa
    13321 gtggagcaga agacagcgaa gtatcctatc atcaggaaat acgggccgat aacctgtatc
    13381 ctgattgcat ggatcccggg gatcggcctg tatggcactc cggtcatcgc atggatgctc
    13441 ggctggaagc ggattccgtc agtgatatgt actgctgccg gatttcttat tgcctcactc
    13501 tttgtcctgt tctttgcaag ccgaattaat gagctgctcc acctcgcggc aattgccggc
    13561 gtgatcgtct atgcagtcac cgtctcgctt tcaatgggcc tgtctcttcc ggtttccctc
    13621 atcaggggat cgctccaaaa accaatcctg ctactctccc tgctcatcgt caatttcatc
    13681 ctggtgccca tagctgcctc tctcatcgtg tcatatctct tccttccctc aggcgcctcc
    13741 accggactgg tcctgctgtc cctctcggca ggagccccat cactctattc ctggctccgg
    13801 caccaagggg gagacggggc attgcttgcg ccacatcttg ccatcctctg cctcgtttcc
    13861 attattttta ttccactcat gcttcccttc ctcttccccg tagggtggga ctatctgtgg
    13921 atggcattct tttatctcgg tatccttgtg cttgttcccc ttctcgccgc actctatttc
    13981 agccccaggc acgaagcagc cgcttcccgg tggagcccgg ttatcgataa ggtctcctgg
    14041 attgcacttg gagtgaccgt catcaccatg ctcatcctct tcaccaccgc tttcttcgaa
    14101 gtcccgggaa caatcagcct cattgcggct gtcctcttcg tattggtggg atacctggca
    14161 gggtatgccg ctggggggcg tgatcctggg gtgcgacgga tttttgcgat ggtcaccgca
    14221 ccgagaaacc tggctgatgc cctgatcgtg gcattccttg tgttcaggga aggcccggtc
    14281 atggtgatgg cattggttgc cggccttatc ggcctcgttc ccttcatgat caactggtgg
    14341 agtatgcgca aacaccgcac atccctgccc aagctgctct gatcgcacct cggttacggt
    14401 gcattcaaaa tgataatcag gtcactgctg atcttggtaa tcacccatgt ctgataaata
    14461 agggtatagt acaatttttt ctacttaccc tgtgattaaa ttacgtctaa aaatatttca
    14521 tttttgcagt tccaaaaatc gatgaaaaaa agttaaatcc catatagcaa ttcacaatga
    14581 aggaataatg tggttgatac catcctatcg catcttgggg gacgccacag cggcgggggt
    14641 aaggcgaagg cgcaaccccc aggagcgtta agaaagatat gaaatcattt gatggcgtga
    14701 aaaaatggtg gatgatatgc cactaaaccc aaaataacat gattccttta aaggccagat
    14761 gcataaaaga tgaaaatgcc agaggcattt tcatataaca aggacgctga tgcttgcaat
    14821 cggcctcgtc gccctttcag caccacttta tatccttcat ttcttatgtt ccataatccc
    14881 catcactttg gcatatttct cccaggggat ctggccttcc tgccgatcga agtcctgctg
    14941 gtgaccctct ttctgcacca gctgcttgag aagcgtgatc gggagatgag gcttgagaaa
    15001 ctgaatatgg tgatcggcac tttcttctca tccatgggga ccagcctccc tgcgtatttc
    15061 tcagatcgcg atctccagat gggtgagata agaaaaagcc ttcggatatc agacagctgg
    15121 agcgaggagg atttcacacg gatgagtgcc gaactgtctg catattccag cagaggtact
    15181 cttaaggata tagacccgtc gggattgcgg aatttcctcc tcgaaaagga ggatttcctg
    15241 atgcggctgc tggaaaatcc ccttcttatc gagcacgaat catttaccga tatgctcctg
    15301 ccggtttttc accttacggg cgaactcacg cacagaaaaa atctcaccgg tcttcctgca
    15361 aacgactagc agcatctcac gaaagacatc tccagagctt accatctcct gatcctccag
    15421 tggctagcat acatgcggca tcagaaacac cactatcctt acctcttctc cctggccttg
    15481 aggacgaatc ctttcgatga agaagcgagt gttatcatcc cctgaacttt tctccataat
    15541 tcaaatgata cttttaaata gaaggattga gggtaaaccc ctgctatgag tacgagccag
    15601 atcatcttaa aagagggaga aaataccttc acgtttgaag tcaccccgga agagataaga
    15661 ttattgaagg attttttcca gcagtatcat gcacggccac tccagagaaa atccaaatta
    15721 aagaatttga aaagttatat ctagcgttcc tttaccctcc cttttgatga gactcatatt
    15781 tttctgggaa gaaatccctg gtatccgcaa taatcagaat acttctggaa ttgttcaatc
    15841 tccctccatg tcatgtcaat tacggcgagg gcatcctgat tttcagcata ctcttcctcc
    15901 atccctataa gcttgatctg gagtggagtg taatactcgt tccaccacgc ctcggaaggg
    15961 aggctgaaac ttgcgacaag atcgtatcct gatttctcaa tcatctgtct gcatccggcg
    16021 ataaaggtga tggggggtat gcctccctca tgaacgttgc tatctcatca ggtgggttct
    16081 cggtaaacca gaccattctt gtgagagcaa gcgagcctcc cgtcttcagg aactttttcc
    16141 agcacgatag tttcttttca aatccaatca caaagatcga tccttcagcc cagacaacat
    16201 cgaaatattc cgagcaaagg ggagatcgtc catcgatctg caaacggtga ctatccgcct
    16261 accggttcct tccgcatctg cccttttccg gagcgtatca cggtaaggct ggtagctgtc
    16321 aacagccgtg atgtggcagt ccctgcactg tcaggcaagg gcgagcgtct ggctgtcggc
    16381 accgcaaccg atatcaagaa tcctagaatc tggagggggg aggggatatt gaacatcctg
    16441 ttcgtgcagg catcgcttcc cggtcccttg cggtcaagcc cttcaaagag cgtgaatata
    16501 aacggtgtat ccatatgctg ctttatccat aagagatcat atccgttccg gagatctcat
    16561 atccatgaaa aagcgacact ccaggagtat gaaaatgata gagacaaaaa attatagaga
    16621 cgacccatca aagacactag ggtccggagg gtggcaggta cagaattttg aaatatccct
    16681 catatgggca ctaattagga aataaaaatc tttatgttgg tattttccca ataattaaaa
    16741 actttcatgg ctgctgcacc agatcccacg tcactcaaag acctcctgtc acgcctttca
    16801 cgccatccgg ctttccttgg tgccctgata ggcatccttg cggcattttc ccagtcgctc
    16861 ctcatcagtg ccggaggccc tgaagcttat gggttctgcg ttgcctgcca tacccgtgac
    16921 cttgtaaacg ggatgaccaa tattatcacc ggcgccagcc tggctcttgc ccccatctca
    16981 aagaatgcca tccttccggt gatgagcgtg gtgggtgtta ttatcggggc attcctctca
    17041 gcaaaggttc acaaggagca taaaatcaag aagactgact atcaggaata cctcatctat
    17101 ttcatcggtg gatttgttgt cctccagctt gccatggtct tcggagggtg cccgtacaga
    17161 gcagccctgc ggacaggata cggtgatatc acagcgctcc tttttatcat catgatgggc
    17221 ctcggagtca ttgcagggac actccttatg cttcg
//
LOCUS       DAOOCO010000014        17169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_2965_length_17169_cov_4.972011, whole genome shotgun sequence.
ACCESSION   DAOOCO010000014 DAOOCO010000000
VERSION     DAOOCO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2965_length_17169_cov_4.972011"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1388)
                     /locus_tag="PK154_01685"
     CDS             complement(210..1388)
                     /locus_tag="PK154_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPW09804.1"
                     /translation="MKVCIMCGGEGTRLRPLTFDRPKPCIPIVNRPSVEHLVSHLSNL
                     GFSEIVITLGYMGILIEQALGDGSLFGVDITYVHEKTKLGTAGSVKNAQEYLDGQDFL
                     VVGGDHVTDINLLEFYRVHMKERPIVSIGLISIDEPSEYGIAEIDVHYQIRRFREKPG
                     PGEIFSNLASTGIYVCNPEIFNWIPRATKFDFARDLFPLLMEKGYGLKGWLARGNWTD
                     VGSPASLRQAERWKLDDIPYTSIRGNISIRGGQILGPVLLGDSISVGRNSQIIGPASI
                     GTGTTIGDEVLIGPYTSIGEHCSIRPGAKIFSSSVYNHVILGKNSTVSGSIIDNDTVI
                     GDNCSLEHDTVIGPRVVIRNRAVIHSKTRLWPEVVVDDDTIVKEHLLNEKFDGRCEGS
                     "
     gene            1497..2345
                     /locus_tag="PK154_01690"
     CDS             1497..2345
                     /locus_tag="PK154_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09805.1"
                     /translation="MELHDDTKKPMTSRERYLSIAVPVLCFGGTALGILLWRAGYIRD
                     PGAFYWGCIAGAILLAYLAWLKPRRDIVSLLAPLYAVLIFLLPLENKPTTLLQLLFGA
                     SLTILVVRLNLRFSTPVKRIGDDPMEKYLYDYMHRITPLYRGIDREIAHDVASAVLSF
                     KFGLYPNTISSADQAITRLTGEGPIATLKKALRILRDRATSLEEFEIREYSKETFGEG
                     DDPYLALKLTPEQVENFEDFTLDNSMVLCYAVAYLYSPDDGQMLDEHQNFILQILNPY
                     KELLGL"
     gene            complement(2564..2884)
                     /locus_tag="PK154_01695"
     CDS             complement(2564..2884)
                     /locus_tag="PK154_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09806.1"
                     /translation="MGGEVFQAQVIKNFFDTITGTDRNLTRIYLCVISLAKLRMENPE
                     KVSFLVDQIRKSKQQRELSVDILDYMVDAAIQLDLNVVQTAFGVKTIGEVMQDFNGIS
                     LDSL"
     gene            complement(3111..4280)
                     /locus_tag="PK154_01700"
     CDS             complement(3111..4280)
                     /locus_tag="PK154_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPW09807.1"
                     /translation="MITLALAGKPNCGKSTFFRAATLAPAEIANYPFTTIDANFGVAF
                     TRTICPCRSLNLHCENCHDGVRFVPVNLVDVAGLVPDAHKGRGLGNQFLDNLRQADAI
                     LHIVDASGSTDSEGNPVDVGSHDPRKDVVFLEYEMTMWIYGILEKHWSKIQRQAQSKT
                     YPVHRGIAEVLTGLRITPEDVKDAEEKAGIDLVHADPDSLIQFCREITRISKPMVPVG
                     NKVDQAPGVIIAALKNEGFLFASAAGELALRNAAQAKLIGYLPGDPGFSLVNESALSP
                     PQKAGLGKIAEVMQKYGGTGVQQAIDHTIRRLLDLIVVYPVEDEHKFSDSKGRILPDA
                     FLMKRGSTPRDLAFMVHTDIGKGFLYAVDARTKMRVKDTHELQDGDIIRIISTAK"
     gene            4323..4913
                     /locus_tag="PK154_01705"
     CDS             4323..4913
                     /locus_tag="PK154_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HPW09808.1"
                     /translation="MSFTARNTYYFDAPGPANTADCARFAAERARDLGIGKVMVASTS
                     GETARIFHAALKGSGLSLIVVTHVVGFSEPGIWEFSPQIASELRQEGVQIVTGTHALS
                     GLERSLSRSQRVGGGSRTEAIAEAFRRVIAVGLKVAVECVLIAADQGVVSPAEEVVAC
                     GGTNSGADTVCVIRPSHTASFFDLQVREIVAMPRVR"
     gene            4916..5746
                     /locus_tag="PK154_01710"
     CDS             4916..5746
                     /locus_tag="PK154_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09809.1"
                     /translation="MVSASLHSALGLLRSPAAWLPGLTLGGIAAAFIVSQYYFGLFIA
                     ERLLVILLILIPFFMAGVLSMVKSGESGIHSFLSGGLTGYFRILLPSLLILFAIIITI
                     MLVLIPLMALGFGETALIFSVMTCTLSILFFTLFSDTAAVLEGRTVFESIRRSVEFVI
                     RNTHSCVVFYLTCLVIGGAIGFGTLLAWTAALYERLVPVSMMTPGEIQAFTPDQFNAL
                     IGQDGILITALIFFAGIALAFSLLYPFKAFFFRDYAGSGEGEPRLQGEYDSKGRWYKY
                     "
     gene            complement(5743..6939)
                     /locus_tag="PK154_01715"
     CDS             complement(5743..6939)
                     /locus_tag="PK154_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00297 family protein"
                     /protein_id="HPW09810.1"
                     /translation="MAEQLGDNLAALLALSFVLIAPYLQPPWVLTIMILLFSSTLYLI
                     TKTRYFAIALIPIALLYGLALLPLLVFCCTITIIVTGELAFRWGAGELPSYLAYLLAA
                     FTGCILVMLYLHQIIPLVILFGVVVAVLLKAILRNREDALMIEALGIAMTMYLIMELN
                     YQADIVLIVAAVLIAFGFGYFSYRTRTADVSGLFSGALIGIILIVFADIRWFLIMLAF
                     FILGSVSTRYHYREKEKMGVEQKHGGARGYLNVFSNGVIAAASAVLWGVTANPLFAAL
                     FVGSVATAAADTMASEIGVTGGEPYMITTFSRVPAGTNGGVTILGETVAAAGAFIISV
                     IAFLLGVIPLPLVVVGTAAGFIGTNIDSLVGALIENKGVFGNAGTNLVATAGGGICGL
                     LLALLV"
     gene            7120..9714
                     /locus_tag="PK154_01720"
     CDS             7120..9714
                     /locus_tag="PK154_01720"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW09811.1"
                     /translation="MSPPKDLPKNYHFAEVEPRWQGIWRDEDNYFDRTSSLPQFVIDT
                     PPPYPTGTFHIGNAFNWCYIDFIARYKRMQGYNVMFPQGWDCHGLPTEVKVEEIHGIT
                     KNDVPREKFREMCRELTIANIGKMRLTLRRLGFSVDWSNEYITMLPAYYKKTQISFLK
                     MLASGYIYQSEHPVNFCTRCETAIAFAEVAYEPRETFLNYFDFDGVEIATTRPELLAA
                     CVAVAVHPKDERYTGISGRHLTVPIFGHRIPIISDEAVDPAFGSGAVMICTFGDKQDV
                     FWWKQHRLELRKAIDRQGRMTPVAGKYEGKKSGECREAILSDMKALGILKRQEPLEQR
                     VGTCWRCKTPIEIMSERQWFVKIAPDEIMKAAREVKWTPGHMFLRMENWIGQMEWDWC
                     ISRQRIFATPIPVWFCRECGTMILPDEKDLPLDPTTRKPGHPCNKCGSTEVVGEEDVL
                     DTWMDSSISVLNVTGWDGTKAPPLFPAQIRPQGHDIIRTWAFYTILRAVALTGKRPWD
                     EILVNGMVLGEDGFKMSKSRGNIISPEEIVEQYGSDAFRQWAAAGAATGSDIMFNWND
                     VIAASRFQTKLWNVARFILLQLEKKEFNHEAPVTALADRWLLARLAETVSEVTEAMEQ
                     YQFDRGLKALREFSWNVLADNYIELVKGRLYSDHPSRDSACRVLWETIDALCRMMAPY
                     APHFSDEIYSHLREGSVHKVAWPEALPSLQEDREKGDLLVQLVSEIRRFKHEQGLALN
                     APLGKVTVFSSRINGDGGDMARALNASIEWCADTGALEKSPGEIVFNMAVIGPALRKD
                     AKAFMDAVRGLPPDLLSSPPATIDIGGRRVEVPAHSFSQQTDYLVGGTRVDLITVGDV
                     IVTVQKHP"
     gene            complement(9671..10288)
                     /gene="tmk"
                     /locus_tag="PK154_01725"
     CDS             complement(9671..10288)
                     /gene="tmk"
                     /locus_tag="PK154_01725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPW09812.1"
                     /translation="MLVTIEGIDGSGKSSLVVSLHTLLSDLSPVFTREPGSTWVGDQV
                     RRAVAEKTDPITEALLFAADHAAHLSTLVRPSLERGLLVISDRYSDSRYAYQQETLKG
                     YIDDPLAWLLALHAGWTVRPDLTFLIVLPVDEALSRIKEKQNREHFENHRLLEKVQDQ
                     YLALAKSDPSRFVLVDGTKDEGEIAGFVAGVIRDAFGRSRSHRRP"
     gene            complement(10279..10986)
                     /locus_tag="PK154_01730"
     CDS             complement(10279..10986)
                     /locus_tag="PK154_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPW09813.1"
                     /translation="MILHANSDYATTMDLVLAMDLKDGFVVHGKKGDRAGYRPLTWGL
                     SPVADPIGYLEVMQPRYLYIADLDRIAGTGSHDPIILSCAGMVERCYVDRGSRSPDDS
                     LEGAHIVNVVGTETAGDLLQYRGGMLSLDIRDELVLPEGRAPEEVLFGAAGLPFDCCL
                     IMNISGVGTGRLFSASLLKRMRNAYGKRLFYGGGVRNGHDLDRLARAGFDGAIVATGV
                     HKGSIPHESVREGYWCS"
     gene            10995..11681
                     /locus_tag="PK154_01735"
     CDS             10995..11681
                     /locus_tag="PK154_01735"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HPW09814.1"
                     /translation="MRLLVSPRDVEEAKRSGAADIIDVKRPAEGSLGANFPWIIRAVK
                     DLSDKPVSAAIGDFDFRPGGAALAAYGAAHAGADYVKIGLMFDGAKRAEEVIGAVVRA
                     VKEEFPEKFVVIAAYSDYERLGTISPFVMSPLAAAAGADVAMVDTGRKDGKSTFEFMN
                     EEELAEFAGMNGAAGMQTALAGSLCFEHIPALKRINPDIIGVRGMVCGGNRNSTIQED
                     LVIRLARMVG"
     gene            11685..13151
                     /gene="guaB"
                     /locus_tag="PK154_01740"
     CDS             11685..13151
                     /gene="guaB"
                     /locus_tag="PK154_01740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPW09815.1"
                     /translation="MYLTKLDAPLALTFDDVLLLPAESQVEPNETDTKTLFSKNIPLN
                     IPLVSSAMDTVTESSMAIALARNGGIGVIHRNMSAEQEAAELRFVKQAAELIERKVLS
                     VSPDTSVAEVERLMNEQGIGGVPVLEEGRVVGIVSRRDVRGISSRRGSEKVRTIMTRK
                     PITAGEDITMEQALEVMYTHKVERLPVIDKEGGLAGIITMQDILEKRQYPNATRGKTG
                     TLRVATAVGPFDFERAMLLDEEGTDALVVDCAHGHNMNVVKSVRNIKGSAKADVIAGN
                     IATAKAAEDLMDTVDGLKVGIGPGSICTTRIVAGVGVPQITAVASVSDVVKDAGIPVI
                     ADGGVRFSGDVAKAIAAGADSVMIGSLFAGSDEAPGRIITMKGRRYKQYRGMGSLGAM
                     SGGVSSDRYFQKKEIGRTKFVPEGVEGVTPYVGSVSDVLYQLVGGLKSAMGYTGSRNI
                     AEMHKKTRFVRITAAGQTESHPHNILITDEAPNYRLSD"
     gene            13297..13914
                     /locus_tag="PK154_01745"
     CDS             13297..13914
                     /locus_tag="PK154_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW09816.1"
                     /translation="MLDHVEVARILDILGNRNRRRIIELLRQKPCFVTEISDRLLLSP
                     KAVIEHLQLMEREEILSFRLDDRRRKYYYLANDISVMVSLHRLEEGTRSSDNVNNQLR
                     KSLGKLRKLVDAREELAGNLRNIEKDIDLQIREIVQQGREVLEDDSGIDLLIALAHCN
                     LTGEELAELTGQPVQNLAAGLAELRKKHIVEQEGPTFRLCGINAE"
     gene            13904..14311
                     /locus_tag="PK154_01750"
     CDS             13904..14311
                     /locus_tag="PK154_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09817.1"
                     /translation="MPNDSYDDFLKHIARMVEDIVRNLPEAEGTRFVGYTIIAGNNGE
                     APHVIHIGKNPREEIEYEVVEDQEYLFITGSLPSSSQCAAYADISTDSVTIIVGEHRA
                     SIPLNTTIDVVHSFYRVRHGIIDIVLKKKRQAP"
     gene            complement(14326..16509)
                     /gene="nrdD"
                     /locus_tag="PK154_01755"
     CDS             complement(14326..16509)
                     /gene="nrdD"
                     /locus_tag="PK154_01755"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPW09818.1"
                     /translation="MPDHKTQQTTLDGIFVPRMPPVRTSDGHIVDWERQRIIRQIVEE
                     TKLVETFYGYESANEDIAQEIARLVEDRICSLRLKALSGPLIREIVNITLLEGGMTQY
                     RNVCTRVGTPVFDAHLIDVGRGFEARDNANLQENAETSHKKKADKISKEQYLLQLPPH
                     LADHHLSGNIHIHDLEYFGTRPFCQDWDLRYFLYYGLMPDGNGTKASVAGPAKRPEVA
                     ILHAVKALGSAQTNFAGGQGYYNFLTFIAPFFDGMPYDEIRQHMQMFVYEMTQMMVAR
                     GGQLVFSSVQLSPGVPSLWKDKPCVYKGKIWDGRSAPLRTYGQFEREVRLLFKALMEV
                     MLEGDYWGKPFSFPKPEISIEPDFINENEEFNREHPDLPTYRDLYLLTFELASKFGTP
                     YYDNQMPEYRGAGKGISCYQCCAYQFSAVADEDTHFEDKLFFRDGKHFSMGSWQVVSL
                     NCPRAAYEAAGDDNRLFAELRALMDICVEVFKIKRRWLEIIRTNGRMPFAMQRPKDPE
                     TGERGAVAVDLDGLVYTIGVVGVNEMVQHHSGSQLHESRDAWVLAIRAMTEMELYAKE
                     LSARNNMTIALARTPAETTGQRFAVADLLKKEYRECALKVIKGDIEQGIQMLGKSRDL
                     PIYYTNGTHVAPSANIPLARRMEIEHVFFPIVDGGNIFHIWLGEQRPDPGGLMDMAMK
                     LCMNTQIGYFAFTRDLTVSLRQFREFRPDKKSISEWVSPGTRIQA"
     gene            complement(16509..16778)
                     /locus_tag="PK154_01760"
     CDS             complement(16509..16778)
                     /locus_tag="PK154_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPW09819.1"
                     /translation="MADPQKLVIYTLEYCPNCELVKDYLNRNDIPFEEKNMATAESLT
                     ELRINGVFVNEAPVLRGPGGFLTTRDLFSKGDLQEERIRRCSEGT"
     gene            16880..>17169
                     /locus_tag="PK154_01765"
     CDS             16880..>17169
                     /locus_tag="PK154_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPW09820.1"
                     /translation="MRNAGKGHFLSGTLGRYRDCSIKERTCMVRLFVAIDLPADIRER
                     LKGPQEHLKKSSARLTSVDPALIHITLKFIGEVPQDAVGRIMTALSGIPFSP"
BASE COUNT         3757 a         4786 c         4940 g         3686 t
ORIGIN      
        1 ccaatagctc ttagatcttt tggtaaacct ctaatttgag gatgtcaaca aggccaaaaa
       61 aatgggatgc ttgtgtgagc cctatcacaa cggggggatg ccacaacagc tgttgaaaac
      121 caaagaagct cgccgaggat aggctctgat gggaggtatc ctcctatcag atgtgaaaaa
      181 aaagggatga aatgccacta tacccttttt taggagcctt cacacctgcc atcgaatttt
      241 tcattcagga gatgctcttt tacgatggta tcatcatcta ccaccacttc aggccacaac
      301 cgggtctttg aatggatcac cgcccggttc ctgatcacca cccgtggacc gatgaccgta
      361 tcatgttcga ggctgcaatt atctcctatc accgtgtcat tgtcgatgat actcccgctc
      421 acggtgctgt ttttcccgag gattacgtgg ttatagacgg aagaggagaa gatctttgcc
      481 ccgggacgga tgctgcagtg ctccccgatg ctggtatacg ggccgatcag gacttcatcc
      541 ccgatcgtgg tgccggttcc aatcgaggcg ggaccgatga tctgagagtt cctgccgacc
      601 gagatcgaat cacccagcag aacgggacca aggatctggc ctccccgaat cgagatgttc
      661 ccccgtatgc tcgtataagg gatgtcgtcg agtttccagc gttctgcctg ccggagggag
      721 gcaggacttc ccacatccgt ccagttgccc ctggcaagcc agcccttcaa cccgtaaccc
      781 ttctccatca ggagggggaa gaggtcacgg gcaaaatcga acttggtcgc tctgggaatc
      841 cagttgaata tctcggggtt gcagacatat atcccggtgc ttgcgaggtt tgagaagatc
      901 tcccccggtc cgggtttttc ccggaaacgc ctgatctggt agtggacatc gatctcggca
      961 atgccgtatt ctgatggctc atcgatactg atgagaccga tcgaaacgat agggcgctct
     1021 ttcatgtgaa cccggtaaaa ttcaagcagg ttgatgtcgg tcacgtggtc cccgcccacc
     1081 acgagaaagt cctgcccgtc aaggtattcc tgggcatttt tgacgctccc tgcggtcccg
     1141 agcttggtct tttcatggac ataggtgatg tcgaccccga agagtgagcc atcgcccaag
     1201 gcttgctcga tcaggatgcc catgtaccca agggtgatca cgatctcgga aaatccgagg
     1261 ttcgagaggt gagagaccag atgttcgacc gaggggcggt tcactatcgg tatgcatggt
     1321 tttggccggt caaacgtgag ggggcggagc ctggtgcctt cccccccgca cataatacac
     1381 accttcatac gtctctgttc aacccgatcg gttttaacta tatggagcgg agccgatcag
     1441 atcggattga aactaattta tgggattctt tccaatagta tgccggaaac cgtactatgg
     1501 aactgcatga cgacacaaaa aaacccatga catctcgtga acgatatctc tctattgcag
     1561 ttcccgttct ctgttttgga ggtacggcac tcggcatcct cctttggaga gccggatata
     1621 tccgcgatcc gggagccttc tactggggat gcattgcagg tgccatcctt cttgcctacc
     1681 ttgcctggct gaaacccagg cgggatattg tatcgcttct cgcacccctc tacgcagtgc
     1741 tcatcttcct gttaccactt gagaataaac caaccaccct cctacagctc ctgttcggag
     1801 cgagcctgac catcctcgtt gtgaggctga acctccggtt cagtactccg gttaagagaa
     1861 tcggagatga tcctatggaa aaatacctgt atgattatat gcaccgcata acacccctgt
     1921 atcgtggcat cgatcgggag atcgcccacg atgtcgcctc ggcagtcctt tccttcaagt
     1981 tcggactcta tcccaacacc atctccagtg ccgaccaggc catcacccgt ctgaccggtg
     2041 agggtcccat agccaccctg aaaaaggcgc tccggatcct ccgggatcgt gccacaagcc
     2101 tggaagaatt tgagatcagg gagtattcaa aagagacctt cggcgaaggc gatgatccct
     2161 accttgccct gaagctcacc ccggaacagg tcgagaattt tgaggatttc acccttgata
     2221 attcgatggt gctctgctat gctgtcgcct acctgtactc cccggatgac ggccagatgc
     2281 tcgatgaaca ccagaacttc atcctgcaga tcctgaaccc gtacaaggaa ctccttggcc
     2341 tgtgaggcaa ggttcctccc ttttccatcg atttcccagg gatacgtcct tcctcatatc
     2401 ttctgccatg ggcattttcc cagaaagaaa ggcagaggga gtaaagagag agatgaaggg
     2461 tctcatctgc caaggggaat ctgcctatga gaaaagaagt gatactctgt cccagataaa
     2521 gagagtattc cgtcaatcct gccagggcac ggggagaaaa ggtttatagg gaatcgaggc
     2581 tgatgccgtt gaagtcctgc atgacctcgc cgatggtctt gaccccgaac gctgtctgca
     2641 ccacgttcag gtcaagctgg atggcagcat ctaccatata atcgagtatg tcaaccgaga
     2701 gctcacgctg ctgcttgctc ttccggatct ggtccacgag gaacgagacc ttttccgggt
     2761 tctccatcct gagctttgcc aggctgatta cgcacaggta gatacgtgtc aggttccggt
     2821 ctgtgccggt gatggtatca aagaagttct tgatgacctg ggcctggaag acctctccac
     2881 ccatattata ttgaggtatt gtatatgagt atttaaagtt atctgataaa agccattatt
     2941 tgcacgtaaa tcccggaaaa aacgtgtctg acgcgattac caatacgctc ccagagcagt
     3001 aagtatctga ttcccctacc tgccctcctt accccactct ggatctgtat ctcatgatac
     3061 caggaagacg gtggggatgg ggtgaagagg agcgggatga gtgcctccgg tcattttgcc
     3121 gtgctgataa tccgtatgat atccccgtcc tgcagctcat gggtatcctt cacccgcatc
     3181 ttagtccggg catcaacggc atacaggaat cccttaccga tatcagtatg caccatgaac
     3241 gcaagatcgc ggggagttga tccccgcttc atcaggaacg catccgggag tatccggcct
     3301 ttgctgtccg agaatttatg ttcatcctcc accggataga ccacgatcag gtcgaggagc
     3361 cttcggatgg tatgatcgat tgcctgctgg acaccggtac cgccgtattt ctgcatgacc
     3421 tccgcgattt ttcccaggcc cgccttctgg ggaggggaga gggcggattc gttgacgagc
     3481 gaaaacccgg gatcacccgg gagataccca atcagctttg cctgggcagc gttccggagt
     3541 gccagttcac cagcggcaga ggcaaagagg aacccctcgt tcttcagggc ggcaataatc
     3601 accccgggtg cctggtccac cttgtttccg accggcacca ttggcttgct gatcctcgtg
     3661 atctcccggc agaactggat cagggagtcg ggatctgcat ggacgaggtc gatcccagcc
     3721 ttctcctcag cgtccttcac atcttcagga gtgatacgga ggccggtgag gacttcagca
     3781 atcccccggt gaaccgggta ggtctttgac tgggcctggc gctggatctt ggaccagtgt
     3841 ttctcaagga tcccatagat ccacatcgtc atttcgtatt cgagaaagac gacatccttt
     3901 ctcgggtcat gactgccgac atcgacgggg tttccctcac tgtcggtcga gccgcttgca
     3961 tccacgatat ggaggatggc gtcggcctgg cgcaggttgt cgaggaactg gttccccagc
     4021 cccctgccct tgtgcgcatc ggggacgagc cctgcaacat caacaagatt gaccgggacg
     4081 aacctgaccc cgtcatggca gttctcacaa tggaggttca ggctcctgca ggggcagatg
     4141 gtccgcgtga aagcgacccc gaaattggca tcgatcgtgg tgaacgggta attggcaatc
     4201 tcagccgggg caagggtggc agccctgaag aacgtggatt tcccgcagtt tggctttccc
     4261 gccagcgcaa gcgtgatcat agcaatttcc tttaagtgat actacctaat taacttgtta
     4321 ttatgtcgtt taccgcccga aacacgtatt attttgacgc gccgggccct gcgaacactg
     4381 ccgactgtgc acgctttgcc gcggagagag cccgggatct cgggattggg aaggtgatgg
     4441 tcgcaagcac atcaggggag accgcccgga tcttccatgc tgccctgaag ggatccggcc
     4501 tgtccctgat cgtggtcacc cacgtggtcg ggttctcaga gccagggatc tgggaatttt
     4561 ccccccaaat cgcctcagaa ctgcgccagg agggggtcca gatcgtcacc ggcacgcatg
     4621 ccctctcagg acttgaacga tcactttccc gttctcagag agttgggggg ggatcgagga
     4681 cagaggcaat tgccgaagca ttcaggaggg tgatcgcggt cgggctgaag gttgccgtgg
     4741 aatgtgtcct cattgctgcc gaccagggag tggtgagtcc tgctgaggag gtcgtggcgt
     4801 gcggtggaac gaacagtggg gccgatacgg tttgtgttat ccggccttcc catacggcat
     4861 cgttctttga cctccaggtc agggagatcg tggccatgcc acgggtccgg taaccatggt
     4921 atcggcttct ctccattcgg ccctgggctt gcttcgttcc cctgcagcat ggctgccggg
     4981 cctgactctc gggggaatag cggctgcgtt catcgtctcg caatattact ttgggctgtt
     5041 tattgctgaa cgactcctcg taatactgct catcctcatc ccattcttca tggcaggtgt
     5101 tctttccatg gtgaaaagcg gcgaatccgg gatccattcc tttctctccg gagggttgac
     5161 tgggtatttc cgtatcctcc tcccgtccct cctgatcctt tttgccatca tcatcacgat
     5221 catgctcgtc ctgattccgc tcatggccct tggattcgga gaaaccgcac ttatcttctc
     5281 agtaatgacc tgtaccctgt cgatcctctt ttttaccctc ttttccgata ccgcagcagt
     5341 gctcgagggg agaacagtct ttgaatcgat ccggagaagc gttgagtttg tcattcgcaa
     5401 cacccactcc tgcgtcgtgt tctatcttac atgcttggta atcgggggag cgatcgggtt
     5461 tgggacgctg cttgcctgga cggcagcact ctacgagcgg ctcgttccgg tctcgatgat
     5521 gacaccggga gagatccagg cattcacccc ggaccagttc aatgccctga tagggcagga
     5581 tgggatcctg atcaccgccc tcatcttctt cgccggcatt gcgctggcct tctcactcct
     5641 ttatccattt aaagcctttt tcttccgtga ttatgcgggc agcggggagg gggagccgag
     5701 actgcagggc gagtacgaca gtaaaggacg ctggtacaag tactaaacga gcagggcaag
     5761 gaggaggccg cagatccctc cccctgccgt cgccaccagg ttcgtcccgg cattgccgaa
     5821 tactcccttg ttctcgatca gcgccccgac aaggctgtca atattggtcc cgatgaatcc
     5881 ggcagcagtt cctacaacca cgagcgggag cgggatgacc ccgagaagaa aggcgatgac
     5941 agagatgatg aaggcccctg cagcagcaac cgtctctcca aggatcgtca ccccgccgtt
     6001 tgtccccgcg ggaacccggg agaaggtcgt gatcatgtag ggctctcctc ccgtcacccc
     6061 aatctcactc gccattgtat ctgctgccgc ggtggcgacg ctcccgacaa aaagggctgc
     6121 aaacagggga tttgcggtca ctccccagag tacggcagag gcggcagcga taaccccgtt
     6181 tgagaagacg ttcaggtagc cccttgcacc accatgtttc tgctccaccc ccatcttctc
     6241 cttctcgcgg tagtggtacc gggtactcac tgatccaagg atgaagaagg cgagcatgat
     6301 caggaaccac ctgatatccg caaagacgat caggatgatc ccgatcaggg ctccggagaa
     6361 gaggccgctg acatcggcag tccgcgtccg gtatgagaaa tacccaaagc caaaggcgat
     6421 gagcaccgcg gcaacgatca gcacgatatc tgcctggtag ttcagctcca tgatcagata
     6481 catggtcatc gcgatcccga gcgcctcgat catcagggcg tcctcacggt tgcggaggat
     6541 ggctttgagg agaaccgcaa ccacgacgcc gaacaggata acaagaggaa tgatctggtg
     6601 caggtacagc atcacaagga tgcatcccgt aaaggccgca aggaggtagg caaggtagga
     6661 cgggagctct cctgctcccc accggaacgc cagttcacca gtcacgatga tggtgattgt
     6721 gcagcagaat acaaggaggg gcaggagggc tagtccatag aggagcgcga tggggataag
     6781 ggcgatggca aagtagcggg ttttcgtgat caggtacagg gtggatgaga acaggagtat
     6841 cataatggtg agcacccatg gtggctggag gtagggtgcg ataaggacaa acgagagcgc
     6901 cagaagtgca gcaagattgt ccccaagctg ttcggccatc ccgtgtatgg ttggtgcctt
     6961 gtgcacaaga ggtttttcaa ggagatctgc acagagcccc gttctcccgt tcccggtatc
     7021 atcagtttcc tcccctcatg ggggacgaca cagaggacgc tcgtcagatc ttcggggtat
     7081 tcgaacagta tatttcatga aaaaacaaat atgagaaaaa tgtctccccc aaaggatctc
     7141 ccaaaaaatt atcattttgc tgaagtggag ccccgctggc agggcatctg gcgtgatgaa
     7201 gacaattatt ttgaccggac ctcctccctt ccgcagtttg tgatcgatac cccccctccc
     7261 tacccgaccg gcacgttcca tatcggaaac gcgttcaact ggtgctacat cgactttatc
     7321 gcacggtaca agcggatgca gggatacaat gtcatgttcc cccagggatg ggactgccat
     7381 ggtctgccga ccgaggtgaa ggtggaggag atccacggta tcacgaaaaa tgatgtgcca
     7441 cgggagaagt tcagggagat gtgcagggag ctcaccatcg caaatatcgg gaagatgcgc
     7501 ctgacgctcc gcagactggg attctcggtg gactggtcca atgaatacat caccatgctc
     7561 ccggcgtatt acaaaaagac ccagatctca tttctgaaga tgctcgcatc cgggtatatc
     7621 taccagagcg agcacccggt aaatttctgc acccggtgcg agactgccat cgcctttgcc
     7681 gaggttgcct atgaaccaag ggagacgttc ctgaactact ttgactttga cggggtggag
     7741 attgcaacga ccaggccgga gctcctcgct gcctgtgttg ccgtggcagt ccatccaaaa
     7801 gatgagcggt atacgggcat ttcagggagg caccttaccg tgccgatctt tgggcacagg
     7861 atcccgatca tctctgatga ggccgtggac cctgcgtttg gatccggtgc cgtgatgatc
     7921 tgcaccttcg gggacaagca ggacgtgttc tggtggaaac agcaccggct tgagctccgc
     7981 aaagccatcg accgtcaggg caggatgacc ccggtcgcag ggaaatacga gggcaaaaag
     8041 tcaggggaat gcagggaggc gatcctctcg gacatgaagg cgctcggtat cctcaagcga
     8101 caggaacccc tggagcagcg ggtcgggacc tgctggcggt gcaagacgcc aatcgagatc
     8161 atgagcgagc ggcagtggtt cgtaaagatc gccccggacg agatcatgaa ggcggcacgc
     8221 gaagtgaaat ggaccccggg acacatgttc cttcgcatgg agaactggat cgggcagatg
     8281 gagtgggact ggtgcatctc gcgccagcgg atctttgcga ccccgatacc ggtttggttc
     8341 tgtagggagt gcggcaccat gatcctccct gatgaaaaag atctcccgct cgatcccacg
     8401 accagaaaac ccggtcatcc ctgcaacaag tgcggttcga cagaggtggt aggagaggag
     8461 gatgtgctcg atacgtggat ggattcgtct atctcggtcc tgaacgtcac cggatgggat
     8521 ggcacaaagg ctccgccatt gttccctgcc cagatcaggc cgcagggcca tgacatcatc
     8581 aggacctggg cattttacac catcctcagg gcggtggcgc ttaccggaaa gagaccatgg
     8641 gacgagatcc tcgtcaacgg gatggtcctt ggcgaggacg ggttcaagat gagcaagagc
     8701 aggggcaaca tcatatctcc tgaagagatc gtggagcagt acggatccga tgccttccgg
     8761 caatgggcag ccgccggcgc tgccaccggt tctgacatca tgttcaactg gaacgatgtg
     8821 attgcggcat cccggttcca gaccaagctc tggaatgttg cccgtttcat cctcctgcag
     8881 ctcgagaaga aggagttcaa ccacgaagct ccggtaaccg ctctagccga ccgctggctc
     8941 cttgcgagac tggccgagac ggtcagcgag gtgaccgagg cgatggagca gtaccagttc
     9001 gaccggggac tgaaagcgct tcgtgagttc tcatggaatg tccttgctga caattacatc
     9061 gagctcgtga agggaaggct ctattcagat catccgtccc gcgactcggc ctgccgcgtc
     9121 ctctgggaga ccatcgatgc cctctgcagg atgatggccc cttatgcccc ccacttttcc
     9181 gatgagatct attcgcatct cagggagggg agtgtccata aggtggcgtg gccggaggcg
     9241 ctgccctctc tccaggaaga tcgcgagaaa ggagacctcc tcgtccagct tgtctccgag
     9301 atacgccggt tcaagcatga acaggggctt gccctgaatg caccgctcgg aaaggtcacc
     9361 gtgttttcct cccgcatcaa cggtgacggg ggtgacatgg cacgggccct gaatgcatcc
     9421 atcgagtggt gtgccgacac cggtgcactc gagaagagcc caggtgagat cgtcttcaac
     9481 atggcggtga taggcccggc cctcaggaag gatgcaaaag cattcatgga tgccgtcagg
     9541 gggcttcccc ctgatctcct ctccagtcca ccggccacaa tcgacatcgg ggggagacgg
     9601 gtagaggtgc ccgcccattc attctcacag cagaccgatt acctggtcgg gggaacgcgg
     9661 gtggacctga tcacggtcgg cgatgtgatc gtgaccgtcc aaaagcatcc ctgataaccc
     9721 cggcaacaaa cccggctatc tccccttcat ccttcgtgcc atcgaccagg acaaagcggg
     9781 aggggtcgct ctttgcaagg gcaaggtatt ggtcctgcac cttctcgagg agtctatggt
     9841 tctcgaaatg ctccctgttc tgtttttcct tgatccgtga caaggcttca tcgacaggga
     9901 ggacaatcag aaacgtgaga tcagggcgga ccgtccaccc tgcatgcagc gccaggagcc
     9961 aggctagcgg gtcatcgatg tatcccttga gggtctcctg ctggtaggca taccggctgt
    10021 cagagtaccg gtcagagatg acgaggagtc cgcgttcgag ggaggggcgg accagggtgg
    10081 agagatgggc tgcatggtcg gctgcaaaga ggagggcttc ggttatggga tccgtcttct
    10141 cggcgactgc cctccgcacc tggtcgccga cccaggtgga ccccggctcg cgggtaaaga
    10201 caggagagag atctgagagg agtgtatgaa gcgacacgac aagggaactc tttccgctcc
    10261 cgtcaatccc ttcaatggtc acgagcacca gtatccctcc ctgaccgatt cgtgcgggat
    10321 gcttccctta tggactcctg ttgccacgat cgctccatcg aacccggccc gggcaaggcg
    10381 atcaaggtca tgcccgttcc ggacaccgcc cccgtaaaag agcctctttc cataggcgtt
    10441 ccgcattctt ttgagaagag aggcggagaa caatctcccg gtcccgaccc cgctgatatt
    10501 cataataagg cagcagtcga acggaagacc tgctgcccca aagaggacct cctctggcgc
    10561 tctcccctcc ggaaggacca gttcgtcgcg gatatcaagc gagagcatcc ccccgcggta
    10621 ctgcagcagg tcaccggccg tctccgtccc tactacgttg acaatatgcg ctccttccag
    10681 ggaatcgtcc ggagaccggg acccccggtc gacatagcac cgttccacca tcccggcaca
    10741 cgagaggatg atcgggtcat gcgatcccgt acccgctatc cggtcgagat cggcgatgta
    10801 gagataccgg ggctgcatca cctcaaggta cccgataggg tctgccaccg gcgataaccc
    10861 ccaggtgagc gggcggtaac cagccctgtc acccttcttc ccatgcacaa cgaacccgtc
    10921 tttcaggtcc attgcaagaa ccagatccat ggtggtagcg taatcactat tagcatgaag
    10981 gatcattaaa tcctatgcga ttgctggtca gcccgaggga tgttgaagag gcgaagcgat
    11041 ctggtgccgc ggatattatc gatgtaaaac ggccggcaga gggatcgctt ggcgcaaact
    11101 tcccatggat catacgggca gtcaaggacc tttcggacaa acccgtcagt gcagcgatag
    11161 gcgattttga tttcaggccg ggaggggctg cccttgctgc atacggggca gcccatgccg
    11221 gggcagatta cgtaaagatc ggcctgatgt tcgacggggc aaaacgggca gaggaggtga
    11281 tcggtgccgt ggtacgggcg gtcaaggaag agttccccga aaagttcgtt gtgattgcag
    11341 cctattcgga ttatgaacgg ctcgggacca tctccccctt cgtcatgtct cccctggcgg
    11401 ctgcagcagg agccgatgtc gcgatggtgg acactggcag gaaggacggg aagagtacct
    11461 ttgagttcat gaacgaggag gaactggctg agtttgccgg catgaacggc gccgcgggga
    11521 tgcagaccgc tctcgccggg tcgctctgct ttgagcatat ccctgcactc aaaaggatca
    11581 accccgatat catcggcgtc cgggggatgg tttgcggcgg aaacaggaat tccacgatcc
    11641 aggaagacct ggtgatacgg ctggcacgga tggtgggatg agggatgtac ctgacgaagc
    11701 tggatgcccc ccttgcgctc accttcgatg acgtgctcct ccttcccgcc gagtcacagg
    11761 tcgaaccgaa tgagaccgat acaaagaccc tcttttcaaa gaatatcccg ctgaacatcc
    11821 cgcttgtctc ctctgccatg gataccgtca ccgaatcctc gatggcgatc gcccttgcga
    11881 ggaacggggg gatcggggtc attcatcgaa acatgagtgc cgagcaggaa gcggccgagc
    11941 tccggttcgt gaagcaggca gcggaactga tcgagcggaa ggtcctctcg gtctctcctg
    12001 ataccagtgt ggcagaggtc gaacggctca tgaacgagca ggggatcgga ggagtcccgg
    12061 tgcttgaaga gggccgggta gtcggcatcg tcagccggcg agatgtgcgg gggatctcca
    12121 gccggcgggg gagcgagaag gtcaggacga tcatgaccag gaagccgatc accgctgggg
    12181 aggatatcac catggagcag gcgcttgagg tgatgtatac tcataaggtt gagcggctcc
    12241 cggtcattga caaggaaggg ggtcttgccg ggatcatcac catgcaggac atcctcgaga
    12301 aacggcagta cccgaatgca acccggggaa aaacaggaac gctgagagtt gccactgcgg
    12361 tcggcccctt tgacttcgag cgggcgatgc tccttgacga ggaggggacc gatgctctcg
    12421 tggtcgactg cgcccacggg cacaacatga acgtcgtcaa gtcggtgcga aacatcaagg
    12481 ggagtgcaaa ggccgacgtc atcgccggga atattgccac cgcgaaggcc gccgaggacc
    12541 tgatggatac ggttgacggg ttgaaggtgg gaatcggacc gggctccatc tgcaccaccc
    12601 ggatcgtggc tggcgtggga gtcccgcaga tcaccgcggt tgcgagtgtt tccgatgtcg
    12661 tgaaggacgc cggcatcccg gtcatcgccg atggcggtgt ccggttctcc ggcgacgtgg
    12721 ccaaggcgat cgctgcaggg gctgacagcg tgatgatcgg gagtctcttt gccggcagcg
    12781 atgaagcccc gggacggatc atcaccatga aggggaggcg ctacaagcag tatcggggga
    12841 tggggtccct tggtgccatg agcggcggag tgtcaagcga ccggtatttc cagaagaaag
    12901 agatcggccg taccaagttt gtccctgaag gggtggaggg ggtgactccc tacgtgggga
    12961 gcgtatccga tgtcctgtac cagctggtcg ggggtctcaa gtctgccatg ggatacaccg
    13021 gttcgcggaa tatcgctgag atgcacaaaa aaacccgttt tgtccggatc actgcagccg
    13081 ggcagaccga gagccacccc cacaatatcc tgatcacgga cgaggcgccg aattaccggc
    13141 tctcggactg attttttttt cccggttctg gtgcgtcttt gtgattttat atagtcagga
    13201 tcagatatcc tgacagggaa aacagcattt ctgtttagga atcattactg cttacaaata
    13261 cctatatata agaaaatccc taacataaag taatcaatgc tggatcatgt tgaggtcgcc
    13321 cggatcctcg atatccttgg taacagaaac cgccggcgta tcatagaact gctcaggcag
    13381 aagccctgtt ttgtcaccga gatctctgat cgcctcctcc tctccccaaa agcggtgatc
    13441 gagcatctcc aactgatgga acgggaggag atcctctcct tccggctcga tgaccgcaga
    13501 cggaaatact attacctggc aaatgatatc agcgtcatgg taagcctcca ccggctggag
    13561 gaggggaccc ggagttccga caacgtcaac aaccagctcc ggaaatcact aggaaaactc
    13621 cggaaactgg tagatgcccg cgaggaactc gccggaaacc tccggaacat agagaaggat
    13681 atcgacctgc agatacgcga gatcgtgcag cagggaaggg aggtgctcga ggatgatagc
    13741 gggatcgacc tgctcatcgc ccttgcccac tgcaacctga ccggcgaaga actggccgag
    13801 ctgaccgggc agcccgtcca gaaccttgcc gcaggacttg cggagttaag aaagaaacat
    13861 atcgtcgaac aggaaggccc gacattcagg ttgtgtggta tcaatgccga atgattcgta
    13921 tgatgatttc ctgaagcaca ttgcgcggat ggtggaggat atcgtccgca acctgcctga
    13981 agcagaaggc accaggtttg tcggatatac catcatcgcg gggaataacg gggaggcccc
    14041 ccatgtgatc cacatcggga aaaatccccg cgaggagatc gagtacgagg tggtcgagga
    14101 ccaggagtac ctgttcatca ccgggagcct gccttcctcg tcgcaatgtg cagcctatgc
    14161 cgatatctcg accgactcgg tcaccatcat cgttggggag caccgtgcat ctatccccct
    14221 taacacaacg atcgatgtgg tgcacagctt ctaccgcgtc aggcacggga tcatcgatat
    14281 tgtgttaaaa aagaagagac aggccccgta acggatctgc ccggatcacg cctggatcct
    14341 tgtgccgggc gagacccatt cagaaatact ctttttatcc ggtctgaatt cgcggaactg
    14401 cctgagcgag acggtcaggt cccgtgtgaa ggcaaaatac ccgatctgcg tattcatgca
    14461 gagcttcatc gccatgtcca tcagcccgcc gggatcgggc cgctgctcac cgagccagat
    14521 atggaaaatg tttcccccgt caacgatcgg gaagaataca tgctcgatct ccatccgcct
    14581 tgcaagggga atgttggctg aaggggcgac gtgggttccg ttcgtgtagt agatggggag
    14641 atccctgctc ttcccaagca tctggatccc ctgttcgata tcccccttga tcaccttcag
    14701 ggcacactca cggtactctt tcttcaggag gtcggcgaca gcaaaccgct gtcccgtcgt
    14761 ctcagcgggg gtccgggcta gggcgatggt catgttgttc cgggctgaca actccttggc
    14821 atacagctcc atctcggtca tcgcccggat ggcaagcacc catgcgtcac gggactcgtg
    14881 gagctgggag ccggaatggt gctggaccat ctcgttgaca ccgaccaccc cgatggtata
    14941 gaccagcccg tcgagatcga cggccaccgc cccccgctcc ccggtctccg ggtccttggg
    15001 gcgctgcatg gcaaacggca tccggccgtt cgtgcggatg atctcgagcc agcgccgctt
    15061 gatcttgaat acctcgacac agatatccat cagcgccctc agctcggcaa agagccgatt
    15121 gtcatctccg gcagcctcgt atgcagcgcg gggacagttg agcgacacga cctgccagga
    15181 gcccatcgag aagtgttttc cgtcgcggaa aaagagtttg tcctcgaaat gggtatcctc
    15241 gtcagccacg gccgagaact ggtaggcaca gcactggtag caggagattc ctttccctgc
    15301 gccgcggtac tccggcatct ggttgtcgta gtagggggtc ccgaacttgg atgccagttc
    15361 gaaggtgagg aggtagaggt cccggtaggt agggagatcc gggtgttccc tgttgaactc
    15421 ctcgttttca ttgatgaaat cgggctcgat gctgatctca ggcttgggga aactgaaggg
    15481 ctttccccag taatcgccct caagcatcac ctccatcagt gccttgaata ggagccgcac
    15541 ctcccgttcg aactgcccgt aggttcggag cggtgcagag cggccgtccc agatcttccc
    15601 cttgtagaca cacggcttgt ctttccagag ggagggaacc cctggagaga gctgcacgga
    15661 cgaaaacacg agttgtccgc cacgggcgac catcatctgg gtcatctcat agacaaacat
    15721 ctgcatgtgc tgcctgatct catcgtaggg catgccatcg aagaacgggg cgatgaaggt
    15781 gaggaagttg tagtatccct gccccccggc aaagttggtc tgggcgctgc caagggcctt
    15841 gactgcgtgg aggatggcga cctccggccg ctttgctggc cctgccaccg atgctttcgt
    15901 cccgttcccg tccggcatca gcccgtagta gaggaagtat cggaggtccc agtcctggca
    15961 gaacggccgg gtgccgaaat actccaggtc atggatatgg atgttcccgg aaaggtggtg
    16021 gtcggcgagg tggggaggca gctggaggag gtactgctcc ttgctgatct tgtcggcctt
    16081 cttcttgtgc gaagtctcgg cattctcctg gagattagcg ttgtcccggg cttcaaaacc
    16141 gcggccgacg tcgatgaggt gggcatcgaa gaccggggtc cccactctcg tgcagacgtt
    16201 ccggtactgg gtcatccccc cttcaaggag ggtgatgttc acgatctccc tgatcagagg
    16261 tcccgagaga gcttttaagc ggagggagca gatacggtcc tcaacgagcc gcgctatctc
    16321 ctgtgcgata tcttcgtttg cactctcgta gccatagaac gtctcgacga gctttgtctc
    16381 ttcgacaatc tggcggatga tccgctgcct ttcccagtca acgatgtgac catcggaggt
    16441 cctaacaggc ggcatccggg ggacaaagat gccatcgagg gtggtctgct gggtcttgtg
    16501 gtcaggcatc aggtaccctc gctgcatctc cggatcctct cctcctggag gtcccccttg
    16561 gaaaagagat cccgggtggt gaggaatcct ccggggcccc gcagcaccgg cgcctcgttc
    16621 acgaacaccc cgttgatcct gagctcagta agcgactccg ccgttgccat attcttctct
    16681 tcaaacggga tatcgttccg gttcaggtag tccttcacaa gttcacagtt cggacaatat
    16741 tcaagcgtat aaataaccag cttttgtgga tctgccaatt tcagcccccc gtttgcattc
    16801 agaccagtac ttgttttccg taaaaaaata tttgttgatc tgtcttgatt atacattgaa
    16861 aagaagataa gagagactga tgcggaacgc aggtaagggg cattttttat ccggcactct
    16921 tggccggtac cgggactgca gcatcaagga gcgtacgtgt atggttcgtc tttttgtggc
    16981 aatcgatctc cccgccgata tccgggaacg cctgaaaggt ccgcaggagc acctgaaaaa
    17041 gagcagcgcc cggctgacct ctgtagatcc ggccctgatc catatcaccc tgaaatttat
    17101 cggggaggtg ccgcaggacg ctgtcggaag gatcatgacg gcgctctccg ggataccatt
    17161 ctctccatt
//
LOCUS       DAOOCO010000015        16828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3051_length_16828_cov_4.998331, whole genome shotgun sequence.
ACCESSION   DAOOCO010000015 DAOOCO010000000
VERSION     DAOOCO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 16828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16828
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_16828_cov_4.998331"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PK154_01770"
     CDS             complement(<1..896)
                     /locus_tag="PK154_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW09821.1"
                     /translation="MSRSAIRALMESSIFLVGIVLVATIILFVTEWIRIDLVAILASL
                     ALAWLGLITPAEAVSGFASNAVITMISVMILGYGIERTGIMSRLANTIMRVAGESEQR
                     ITATISLTVGLLSSLMQNVGAAALFLPATRRIGKQTRIPASRLIMPMGFAAILGGCIT
                     MIGSGPLIVLNDLLVQAQEEPFSLFAVTPVGIPLLIAGIALFAVAGSRLLPGKEEEAP
                     PQTIAEVWGIDHRIRTCTLPPSAPLAGRTRGEAECKILYGLDLLAIRAGQDITIAPPR
                     STRFEPGQELAFLGSEEDFKRFL"
     gene            complement(982..2949)
                     /locus_tag="PK154_01775"
     CDS             complement(982..2949)
                     /locus_tag="PK154_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW09822.1"
                     /translation="MESIFLLELMVILILSLLVLFVCNYLKIPLIIGYILSGVLVGPF
                     VLGFVGSTQIIELVAEIGVIFLLFTIGIQYSLKSLFEAKKLVVLGGALQMVITTLCVM
                     ALALLFGTPANVAVFLGLLVSLSSTAIVMKVLQERGELESLHGKTTLSIMIFQDLMLI
                     PLMFITPLLAAPENYQAPSIIFLLLTGFLIIGLVIISTRFIVPRLLYHSAKMKSQEIF
                     LLSVIVICFAIAWIISQTGISLALGAFIAGLMISESEFSVAALSHIMPFRDVFAGFFF
                     ISIGMLLDVAFFLSEPLLILALAVSVILLKCFTGTLTTIFLNLSRKTAILTGLALSQI
                     GEFSFILAKAGESSGLISGSMYQTILDVTLFTMVATPFLMAGSHRIAGRISDRMEDIM
                     GPSCGFDLTRAPATENHLIIVGYGLNGRNVARAAKYAGIPYRIIDINPDMVRTECDQG
                     EPIFYGDGTQAAVLELAGIRTARIIVVAIADRISTQQVVQSARSLNSQAHILVRTRFV
                     SEVEPLHTLGADEVIPEEFETSIEIFTRVLRDYLVPTSEVNQLVEEIRGENYEMLRSL
                     APARLSQIPAHIPGLDIASLRVSGMNPVVGKSLAELPWRQEKGISVIAIRRGDQMLLN
                     PGGDTIIEKGDVAIIVGSTENVRKFIRESGS"
     gene            3131..5215
                     /locus_tag="PK154_01780"
     CDS             3131..5215
                     /locus_tag="PK154_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW09823.1"
                     /translation="MITGYLHDIIIIFAFAIIIVAVGKKFRIPGIVGFILTGILIGPY
                     GLGFIGDTEIIDVLAEIGIIFLLFTIGMQFSFRTLYEMRKIVLIGGSLQVVLTIAATM
                     AISYLFGVTTAQALFFGFLVCHSSTAITLKLYQDRAEIESPQARVTLGISIFQDISTI
                     PMLIALPILAGETTDIFGALLEMSITLVLLFAAVLVISVYVIPRFMNRITGIRSPEIF
                     LLAIVLICFVVTYVADSLGLSLAIGAFLAGITLSESDYFHQAFASIIPFRDIFTSFFF
                     ISIGMLLNVKFLVEAPLLIIALVAGVIVVKAVIAGGAALIIRQSLRTSVLAGLALSNI
                     GEFAFVLSIPGMAYGLFSAASEQIFLAVTLLTMSLTPLAIVSGPRIADLFCRLPLGSR
                     LKAGCEVDRDDGVPPMKDHVVIVGYGLNGRNLAKGARVGGIPYIIIDMNPETVMKEGK
                     AGEQIFFGDATNATILTHASLEQARILVIVINDPVSTRSITLLARQMNPDLYIIVRTR
                     FLTEVTRLRDLGADEVIPEEFETSVEIFTRVLKKYLTPEPTIDRYVQEVRADTYQMLR
                     SPSGYMASISDLRMNLPNLTICTLVVEPGAPVSEKTLGDINVRKQFSVTVLAIRRGTE
                     VIENPSGETDLKAGDDVVVIGTPDKIRDIAGIFRASLVTSDAEGDGRQGGGSGPGDDV
                     SNRSEKKDASAR"
     gene            complement(5233..5376)
                     /locus_tag="PK154_01785"
     CDS             complement(5233..5376)
                     /locus_tag="PK154_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09824.1"
                     /translation="MSILAFLYSEYRRLEEEVKHLGEHRKWDEKTHQYYSYLKQIFHN
                     KNG"
     gene            complement(5576..6394)
                     /locus_tag="PK154_01790"
     CDS             complement(5576..6394)
                     /locus_tag="PK154_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW09825.1"
                     /translation="MDSIPPVLIASLNTLGLTNDEARVYATLVLFDYAEAKEIVEYLA
                     LSKPSVYKALETLDRRGLALRQKSKPVRYRAISPEMAVSILLKEHESASGQALSELKK
                     LERKSVRTESEEPLWTIYGEANTAHKIQELFRKAKREITLIIGERYLPALDHLPAPAI
                     PLLLTIISSSPDTVERMQKKFPGPQVRIKVIPISRLTSPPIGVVFPEIGEVWKYLQFE
                     NVLELNVDDEELLLNAAFFSRGSSVLNTRNKGAIIQLKILSQLFWNRFADDKRK"
     gene            6673..7965
                     /locus_tag="PK154_01795"
     CDS             6673..7965
                     /locus_tag="PK154_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW09826.1"
                     /translation="MRSVGLIALVLACIAIGPVQAGERYMTGSPKLTAAVSGTNEFSP
                     GDNAVIAVQLQNEGLNELKFVGSGIITRDDQPNTAKLITITLAPGASPLIIKSDPQMI
                     GDLKGGSSTTVKFNVKIPKNATVGTYDLPLNLKYTYLWMAEQIGTDSIQYLYRDVNET
                     LSLPVKIKPVLQFSLISSDSEHLNAGTEGYLTLVINNTGQQDGKNTIVKIARNDNSMV
                     IPTDSSSYIGDFPVGAEVTSAFKISVSNEAENKSYPLDVYIRYENNEGDTVDSERTTI
                     GIPVGSKTEFAVISEPIEIPAGQKKTVTVRYQNAGNAPVYSAQARISAVDPFTSNDDT
                     AFLGTIAPGEVKEATFEISVDSTATQKEYGLDSEVRYRDALDNSIISDPIKVRIEVVE
                     RSNALLYLLIAIIALGIIAGGYYYYQRRQKGNGEVKSG"
     gene            8039..10351
                     /locus_tag="PK154_01800"
     CDS             8039..10351
                     /locus_tag="PK154_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HPW09827.1"
                     /translation="MIAAIFEGIARSINRKPLLVAGLVAALFCVAIFGMTQITMESGW
                     KTYLDESSEKGIVYADYIEHFQPDSTIILIIETSDPLDPAIVSYLDALETDIRQQEHI
                     KGTQSIVEILKSANGGTLPASRAEIDRIVGALPSATRNLVTPSNSLTLVQVQLEESVS
                     EDVQRAAMNNVISLVDHTEKPPGLTVEFSGQPAFMQQMEEGLSSNMGILIGGAMLLMV
                     IAMAFLFAYVRYRFMPVLLVGIGLVTALGMMGLAGIGLNMAVIGAFPVLIGLGIDYAI
                     QFHARFDEEARKGSLEDAVFTTVTKTGPAVLYAMLATCMGFVAMFISDVPMIRAFGLV
                     AIIGIFTSYWASCIGVPTLCLLLKYRPKEPKTETCYAVGTDACDFVMDSRKNRNGKKK
                     SFSYREFLTGVSVKIARNPLPILLVLGIIAGVGFAIDPSIPIQTNEDSFVPGDMPAKI
                     NIDKVTRIIGSTSTADFYVRGYGTDLDTIRWIQKFQDYELSHHPELTGAQSIVSAVLS
                     YHGGSLPETQAELDEVLASLPEGTRDEYLGGSLSGVVKFSTIDLDMTQRDSLKQQMID
                     DITFLEPPAGISVAPIGSFDLYTMLLSSLADSKEAMTYLGFIFVFIFLVLVYRHLHAI
                     TPMIPIIFIVGWNAVAMYLLGIAYTPLTATLGSMTIGVAAEYTILVMERYAEEKERLG
                     DNLAAIQESVHKIGTAITVSGLATFCGFSALCLATFPIVSNFGYTTLIAVGFSLCGAI
                     VVMPAVLSVMGRFEDRFHHQVEDRSSAPES"
     gene            complement(10561..11049)
                     /locus_tag="PK154_01805"
     CDS             complement(10561..11049)
                     /locus_tag="PK154_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09828.1"
                     /translation="MIRVKDIPIATRWTMATNVLTRLVVALGYEPVSGTKVPSFRNSM
                     FSAMSREIRAIADRYGMPRENASDLVQTLGAISVILFGPEFETRYIEGFPDEAVIRLT
                     GCAMFREERMRDVTHHQVSSICQAYVEHAIQGLNPGYTITVTKARCRGDPYCEMVIGR
                     KE"
     gene            11159..11362
                     /locus_tag="PK154_01810"
     CDS             11159..11362
                     /locus_tag="PK154_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09829.1"
                     /translation="MNITVLTAGTLPSGNGDAVPGEVDTVNTVAVACKHTDKEGYGAP
                     FLHHPGKPGNKAYIGKEPTRIEE"
     gene            11398..11940
                     /locus_tag="PK154_01815"
     CDS             11398..11940
                     /locus_tag="PK154_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPW09830.1"
                     /translation="MLPLTIFIARIVETSLETIRTVYVARGHKYLAAGIGVGKIAIWL
                     LSTGLVLTNLDNIGGIIAYIAGYGIGTVIGMEIEDRISLGDVVVRIISAKDPQPLITR
                     LSSMGYGITRLDGTGVFSSSVAVLLMVVPRKNLEQLTALLRKDYPDLLYTVEDVRKAS
                     EGGTIFHGKESGWISRFFGW"
     gene            complement(12063..12767)
                     /locus_tag="PK154_01820"
     CDS             complement(12063..12767)
                     /locus_tag="PK154_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09831.1"
                     /translation="MSRITVVAEKEVRQLFKSRNVMLSALIFVVVFGIFTIPAALGGM
                     GEAGNAMDTLGFYLVLTLGIFMGYLFTAQVFLREKQSGVIETLLCTPLSLRELWSGKV
                     AGVTIPATLLTYFSAALITGTASIFTGETLFPSGPVLFHIIIVVPAFIAATSGLIGFT
                     QFMLGMRENQILNFGIIFGIIFLITFVRELAGPGFSVTWGVVAATAGLALLIILATAW
                     LTRFLSRERIMVTLRE"
     gene            complement(12764..13696)
                     /locus_tag="PK154_01825"
     CDS             complement(12764..13696)
                     /locus_tag="PK154_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09832.1"
                     /translation="MAVIDVEFLSKNFSGRKILDEITFSVEKGEIFGYLGPNGAGKTT
                     TLRVLLGLLSPTSGSATVLDADLAKDDATRNRVGVLMENNGLYDQLTARENLVYYARL
                     YEVIRPADRVTELLEFVGLTGREKDLVGNFSTGMKRKLGIARALLHRPEILFLDEPTS
                     GLDPEAQHMVRELILRLSQAESMTVFLNSHNLDEVQRTCSRVAILHQGRIRALDSVRH
                     LTSARGKTGFSVTLADPGQAGKAMDILSSVEGVDDVARNENVLGILLVTVPPHEIVAA
                     LVRNGIQVEEARIQKRSLEEIYLDVMRQAEGGVA"
     gene            complement(13683..13832)
                     /locus_tag="PK154_01830"
     CDS             complement(13683..13832)
                     /locus_tag="PK154_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09833.1"
                     /translation="MPLPSSSPVLLTYIGDITMRSINTIRSGAGHHRKDTLFPEKGVS
                     VPGGN"
     gene            14204..15010
                     /locus_tag="PK154_01835"
     CDS             14204..15010
                     /locus_tag="PK154_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09834.1"
                     /translation="MEILILILLPAVLLSGCMAPSPAVDDSGPVTEIPSGAAPGPAGD
                     NCRLDHLAGRGDEYLSNGDSCYFGTHRPLDFLDDLRMRPHQPVMVLTVPDGWITRDDA
                     ELLMQEIDSEESAAPVVSPLSSYWPYDQTSTVGNEALFLLEGYRTGRYPPRLCSLYYF
                     KPDRSEVRSWWETYGRTGSIDERDAIRKLQETYPDLSAYSSEGMPPRSIRTEQADGGW
                     YVAFIQEGSGFPILSARCYYVDNNGSTRLTRFVNQSIMVLPEDLSPRRCG"
     gene            15328..16812
                     /locus_tag="PK154_01840"
     CDS             15328..16812
                     /locus_tag="PK154_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPW09835.1"
                     /translation="MQPSIEYILLVIAVLILVSILANKVSGRLGVPALLIFLLVGMLA
                     GSEGPGGIYFDDPWIAQAVGVIALTYILFSGGLDTRWSEVRPVLPQAAILSTFGVLLT
                     ALLMAGLAIVVFEVSLLEGVLLGAVVSSTDAAAVFSILRTKRASLKGMLRPLLELESA
                     SNDPMAVFLTIGAITLLLNPNLSPLILIPLFIQQMAVGGLIGYGMGKAMVYLINRLKL
                     EFEGLYPVLTLALVMMTYGVTASLGGSGFLAAYIAGLVLGNSMFIHKNSLMRFHEGIA
                     WLMQIAMFLTLGLLVFPSHLIPVIGSGILLSLFLIFVARPVTVFLTLSPWKMALRDKV
                     MVSWVGLRGAAPIVLATFPLLAGIPQAETIFNLVFFLVITSALLHGTSIPFVARHLGV
                     SDPLHETPRLSMSLGDGCSSGNDLIELTVPIQSGVVGMQIVDIGLPPGTLIIMIEKAG
                     NRFVPCGSTVLEGGDHLLVLTNQPQSNQVRSIFIPEPFAENTGP"
BASE COUNT         3697 a         4716 c         4653 g         3762 t
ORIGIN      
        1 aggaaccttt tgaagtcctc ctcggatcca aggaaggcga gttcctgccc gggctcaaaa
       61 cgggtggatc gggggggtgc gatggtgata tcctgtcccg ctctgatcgc aagaaggtca
      121 aggccataca ggatcttgca ctcagcctct ccacgagttc ttcctgcaag gggagcggag
      181 ggggggaggg tgcaggtcct gatccgatgg tcgatccccc agacttcggc aatagtttgc
      241 gggggtgctt cttcctcttt acccggcagg aggcggctcc ctgccactgc aaaaagcgca
      301 atgccggcga tgaggagcgg tatccctacc ggcgtgaccg cgaagaggga gaagggttct
      361 tcctgagcct ggacgagaag gtcattcagt acgatcaagg gaccggaacc gatcatcgtg
      421 atacatcccc cgaggatagc cgcaaaaccc atgggcatga tcaaccggga tgcagggatc
      481 ctggtctgct tcccaatcct ccgtgtggca gggaggaaaa gggcggctgc accgacattc
      541 tgcatcaagg aggagaggag gccgaccgtc agcgagatgg tggcggtgat acgctgttcg
      601 ctctcccctg ccaccctcat aatggtgttg gcgagccggg acatgatccc ggtcctctcg
      661 atcccgtaac ccaggatcat cactgatatc atggtgatga ccgcgtttga ggcaaacccg
      721 gaaaccgctt ctgccggggt gatgaggccg agccaggcca gcgcaaggct tgcgaggatc
      781 gcgacgagat cgatccgtat ccactcggta acaaacagta ttattgtcgc aacaagcacg
      841 attccgacaa gaaagatact gctctccatc agtgcacgta ttgcagatct ggacatgaaa
      901 ggaaaaaaga gtagctttcc ttgcaccgga tttcaactga ctctggactc gggtggggta
      961 gcccgtcgaa tgctggccct gtcagctgcc agactcccgg atgaatttcc ggacgttctc
     1021 cgtactacct acgatgattg ccacatcacc cttctctatt atggtatccc ctcctgggtt
     1081 cagcagcatc tggtctcccc tccggatggc aatgacactg atccccttct cctgtcgcca
     1141 ggggagttca gcaaggctct ttccgacgac aggattcatc ccggagaccc tcagggaggc
     1201 aatatccagc ccaggaatat gtgccgggat ctgggagagt cgtgcaggag caagactcct
     1261 gagcatctcg tagttttcgc cgcggatctc ttccacgagc tggttcactt cagaggtcgg
     1321 gaccaggtag tcccgcagca cccgcgtgaa gatctcgatc gaggtctcga actcttccgg
     1381 gatcacttca tccgcaccga gggtatggag gggttcgacc tcgctgacaa accgggtcct
     1441 gacaaggatg tgtgcctgcg aattcaatga tcgtgcgctc tggaccacct gctgggtgct
     1501 gatccggtca gcgatggcga caacgatgat ccgggcagtc cggatccccg caagttccag
     1561 cactgcagcc tgggtaccat ccccataaaa gataggttca ccctgatcgc attcagtgcg
     1621 gaccatatcg ggattgatat cgatgatgcg gtaggggatt cctgcatatt ttgctgcccg
     1681 tgccacattc ctcccattga gaccataccc gacgatgatc aggtggttct ctgttgccgg
     1741 ggcccgggtg agatcaaacc cgcaggacgg ccccatgata tcctccattc tatcagatat
     1801 acgcccggca atacgatgtg atcctgccat gagaaaggga gtagcaacca tggtgaacag
     1861 ggtcacatcg agaatcgtct ggtacatact gccggatatc agcccggaac tctctcctgc
     1921 ttttgccagg atgaatgaga attctccaat ctggctgagt gccagcccgg tcaggattgc
     1981 tgtcttccgg gagaggttca gaaagatggt ggtcagggtc ccggtgaaac atttcaggag
     2041 aataacgctg acagcaaggg ccaggatgag gaggggttca gagagaaaga acgctacatc
     2101 caggagcatc ccgatcgaga tgaaaaagaa tccggcaaaa acatctctga aaggcatgat
     2161 gtggctcaaa gcagcaacgc tgaattccga ctcagaaatc atcaggccgg caatgaatgc
     2221 accgagtgca agggatatcc ccgtctggct gatgatccag gcgatcgcaa aacagatgac
     2281 aatcacactg agaaggaata tctcctggct tttcatcttc gccgaatggt agagcaatcg
     2341 tggtacgatg aaccgggtgc tgatgatcac taacccgatg atgaggaatc ccgtgagcaa
     2401 caggaagatg atggatggcg cctggtagtt ttccggtgca gccaggaggg gggtgatgaa
     2461 catcaggggg atgagcatga ggtcctggaa gatcatgatc gagagggtgg tctttccatg
     2521 aaggctctcg agctctcccc gctcctggag cactttcatg acgatcgccg tgctgctcag
     2581 cgataccagg agaccgagaa aaacggctac gttcgcgggg gtcccaaaga ggagagccag
     2641 cgccatgacg cagagggtgg tgatgaccat ctgcagggct cctccgagga ccaccagttt
     2701 tttcgcttcg aaaagacttt tgagcgaata ttgaattcca atagtgaaca ggagaaaaat
     2761 taccccgatt tctgcaacca gttcgatgat ctgagtactt cccacaaatc ccaggacaaa
     2821 aggtccgaca aggactccgg agaggatata cccgataata agcggtattt tgaggtaatt
     2881 gcagacaaag aggaccagta aggagaggat caggataacc atcagttcaa gaagaaaaat
     2941 ggattccatt aaccttagtg gggtagtacc gtaataagaa tttcatttcc ctcactcgat
     3001 ttccagcagg aatccggtat ctgtcacctg gcagggaacc tcaccggaag cgatcagaaa
     3061 agacaaggtt aagaggcttt gacatatagt ctctggtacc gttttaccct tatccattgc
     3121 aaggagcacc atgatcaccg ggtatctcca cgatatcatc atcatcttcg cattcgcgat
     3181 catcatcgtc gcggtcggaa aaaagttcag gatacccggc atcgtcggat ttatcctgac
     3241 cgggatcctt attggcccgt acgggctggg attcatcggg gataccgaga tcattgatgt
     3301 actcgccgag atcgggatca tcttcctcct cttcacgatc gggatgcagt tctcgttccg
     3361 gactctttat gagatgcgga agatcgtgct gatcggcggg agtcttcagg tggtcctgac
     3421 aattgccgcc accatggcga tctcgtatct gttcggggtg acgaccgccc aggcgctctt
     3481 cttcgggttc ctcgtctgcc atagcagtac agcgatcacc ctcaagctct accaggacag
     3541 ggccgagatc gagagccccc aggcccgggt tacgcttggg atatcgatct tccaggacat
     3601 cagcaccatc ccgatgctga tcgccctgcc gatccttgcc ggagagacca cagatatctt
     3661 cggggcactc ctcgaaatga gcatcaccct ggtcctcctg ttcgctgcag tactggtgat
     3721 atcggtgtac gtgatccccc ggttcatgaa ccgtatcacc gggatacgga gcccggagat
     3781 cttcctcctt gcgatcgtcc tgatctgctt tgtggtcacc tatgtggcag acagccttgg
     3841 cctgtcgctt gccatcgggg cgttccttgc cgggatcacc ctctcggagt ccgattattt
     3901 ccaccaggcc tttgcgagca tcatcccctt ccgtgacatc ttcacgagct tcttcttcat
     3961 ctccatcggg atgctcctca atgtcaagtt cctggtcgaa gccccgctgc tgatcatcgc
     4021 ccttgtcgca ggcgtgatcg tggtgaaggc agtcatcgca ggaggggcag ctctcatcat
     4081 ccgccagtcg ctccggacct ccgtcctcgc tgggcttgcc ctttccaaca tcggcgaatt
     4141 tgcgtttgtc ctctcgatcc ccggaatggc gtatgggctc ttctctgctg cgagtgaaca
     4201 gatcttcctc gcggtcaccc tgcttacgat gtccctcacc ccccttgcca tcgtgtcagg
     4261 accgaggatt gccgatctct tctgccgcct tcccctgggc agccggttga aagccgggtg
     4321 cgaagtggac cgggacgatg gcgttcctcc gatgaaggat cacgtggtca ttgtcgggta
     4381 cggcctgaat ggccggaacc ttgcaaaggg agcacgagtc gggggcatcc cctacatcat
     4441 catcgatatg aaccctgaga ccgtgatgaa ggaaggaaag gcaggggagc agatattctt
     4501 cggtgatgcg accaatgcca ctatcctcac ccatgcctcc ctagagcagg ccaggatcct
     4561 tgttattgtc atcaacgatc ccgtctcgac gcgatcgatc acccttcttg cccgccagat
     4621 gaaccctgac ctgtacatca tcgtcagaac ccggttcctc accgaggtga cacgactcag
     4681 ggaccttggt gccgatgagg tgatcccgga ggagttcgag acatcggtgg agatatttac
     4741 ccgggtgctg aaaaagtacc tcacgccgga acccaccatc gaccgctatg tgcaggaggt
     4801 gcgggccgac acgtaccaga tgctccgcag cccttccggg tatatggcaa gcatctccga
     4861 tctccggatg aatctcccga acctgacgat ctgcacgctt gtcgtggaac cgggtgcccc
     4921 ggtatctgaa aagacgctcg gtgacatcaa tgtcaggaaa cagttttcgg tgaccgtcct
     4981 tgcgatccgg cgtggcaccg aagtgataga gaatccctcc ggggagacgg atctaaaagc
     5041 cggggatgac gtagtcgtga tcggaacacc tgacaagatc agggacattg ccgggatatt
     5101 tcgtgcgtcg ttagtgacca gtgatgccga aggagacggc aggcagggag ggggatcagg
     5161 accgggggat gacgtttcga atcgctccga aaagaaggat gcttcggcac ggtaacgtaa
     5221 tcctgggaac catcagccat ttttattgtg gaatatctgt tttaagtagg agtaatactg
     5281 gtgagtcttc tcatcccatt tcctgtgttc cccaagatgc ttcacttcct cttccagccg
     5341 ccggtattct gagtagagga acgcgaggat actcatggag ggagatatag cggccccctg
     5401 gttaaatcct tgatcatggg gatcccgtac cccacggcac caccgtaaaa aataacatga
     5461 ttcctccgag ggccacaagc ataaaagatg aaaatgccgg aggcattttc caatttaaaa
     5521 ggggatagtc gtggaaaaac ggctccaccg atcaggaaag aaacagtgcc ggcatttatt
     5581 tcctcttatc atcagcaaac cggttccaga agagctggga gagaatcttc agctggatga
     5641 ttgccccctt gttcctggta ttcagaacgg atgatcccct ggagaagaat gcagcgttca
     5701 gcaggagttc ttcatcatcg acattcagct cgagaacatt ctcaaactgc aggtatttcc
     5761 agacctcacc gatctccggg aagacaaccc cgatgggcgg ggaggtgagc cgggagatcg
     5821 ggatgacctt gatcctgacc tgcgggcccg ggaacttttt ctgcattctt tcaacggtgt
     5881 ccggggacga ggatatgatc gtcaggagaa ggggaatggc cggtgcaggg agatggtcga
     5941 gggcagggag atatcgttcg ccaatgatca gggtgatctc tcgtttagcc ttcctgaaca
     6001 gctcctggat cttgtgcgcg gtgttcgcct caccatatat cgtccagagg ggctcctcac
     6061 tctcggtccg cacgctcttt cgttcgagtt ttttcaactc cgagagagcc tggccggatg
     6121 cgctctcatg ctctttcaag aggatggaga ctgccatttc gggagagatg gcacggtacc
     6181 ggacgggttt tgatttctgc cgtaaggcaa gacccctccg gtccagggtc tcgagtgcct
     6241 tgtagacgct tggtttggaa agggcgaggt attcgacaat ctcctttgcc tctgcgtaat
     6301 cgaagagtac cagcgtcgcg taaacccgtg cctcatcgtt ggtcaatccc agtgtgttca
     6361 gggatgcaat cagcacggga gggatgctgt ccataccagt aggtgcgtat cctgtgttaa
     6421 aggagttctc cccagattcc gatgccatga aacctcgatg cattcacact aatattgttg
     6481 taaaaatatt tactactatt ttgcgaacct ttttaaggtt cttggtagac attggtggaa
     6541 caagtggagt acctgatact tcgtactctg tgggtaacca tgtccagaca agcacctctc
     6601 ccgggatcga aacccggttc acacacaatg cgcccgtgga atgtaccatc acgggaatcc
     6661 ctgcggtacg tcatgagatc ggtcggtctc atcgcgctcg tactcgcatg cattgcgatc
     6721 ggacccgttc aagcaggtga acggtatatg accggatcac cgaaactgac cgccgcggtc
     6781 tcagggacga acgagttttc ccctggagat aacgcagtga tcgccgtcca gctccagaat
     6841 gagggtctca atgaactgaa gttcgttgga tccggtatca tcacccggga tgatcaaccc
     6901 aacactgcaa agctgatcac gatcacgctc gcacccggtg catcccctct gatcatcaag
     6961 agcgatcccc agatgatcgg agacctcaag ggagggagca gcactaccgt gaagttcaac
     7021 gtgaagatcc cgaagaacgc aacggtcggg acctatgatc tccccctgaa cctcaagtac
     7081 acctacctct ggatggctga acagatcggg accgacagta tccagtatct gtaccgggat
     7141 gtgaacgaga ccctctctct cccggtgaag atcaagccgg tgctccagtt ctccctcatc
     7201 tcctccgata gcgagcacct gaatgcaggg acggaagggt accttaccct cgtgatcaac
     7261 aataccggcc agcaggacgg gaagaacacg atcgtgaaga ttgcaaggaa cgacaacagc
     7321 atggtcatcc cgaccgattc cagctcctac atcggggact ttccggtggg cgcagaggtt
     7381 acctctgcgt tcaagatctc ggtgagcaac gaggctgaga acaaatcgta tcccctcgat
     7441 gtctacatca ggtacgagaa taacgagggg gatacggtcg atagtgaacg gacaactatc
     7501 gggatcccgg ttggcagcaa gacagaattt gccgtgatct ccgaacccat cgagattccc
     7561 gccggtcaga agaagacggt taccgtccgg taccagaatg ccggcaatgc acccgtgtat
     7621 agtgcacagg cacgcatcag tgcagtcgat ccgttcacca gcaatgacga taccgcattc
     7681 ctcggcacca tcgcacctgg cgaagtgaaa gaggcgacgt tcgagatctc ggtcgatagt
     7741 acagcgacac agaaggaata tggcctggat tctgaggtcc ggtaccggga tgcccttgac
     7801 aacagcatca tctctgatcc gataaaggtc cggatcgaag tggtcgagcg atccaatgcc
     7861 cttttgtatc ttctgatcgc gatcatcgct ctggggatca tcgcgggcgg gtattactac
     7921 taccagcgcc gtcagaaggg aaacggggaa gtaaaatcag gataattttc cccgtcattt
     7981 catcaccgcg acggaaacga ggtatgacag ataacaggag gacgtccctg aaatacccat
     8041 gatagctgcc atttttgagg gcatcgcccg ctcgatcaac cggaaaccgc tcctcgtagc
     8101 agggctggtc gcagccctgt tctgtgtcgc catcttcggc atgacccaga tcaccatgga
     8161 gagcgggtgg aagacctacc tggatgagtc gtctgaaaag ggaatcgtgt atgcagatta
     8221 tatcgaacat ttccagcccg attcgaccat catcctcatc atagagacca gtgatccgct
     8281 ggatcccgca atagtgtcat atcttgatgc cctggagacc gatatccggc agcaggagca
     8341 tatcaagggg acccagagta ttgtcgagat cctcaaaagt gccaatgggg ggacccttcc
     8401 tgcctccagg gcagaaatcg acaggatcgt cggggcactt ccatctgcta cgcgcaatct
     8461 ggtcactcct tccaacagcc tcactcttgt ccaggtccag ctcgaggaga gcgtctccga
     8521 agatgtccag cgggcggcga tgaacaacgt gatctccctc gtggatcata ctgaaaaacc
     8581 ccccggcctg accgtggaat tctccggaca accggcattc atgcagcaga tggaggaggg
     8641 cttatcaagc aacatgggca tcctgatcgg gggggcaatg ctccttatgg tcatcgcgat
     8701 ggcgttcctc tttgcctacg tccggtaccg gttcatgccg gtcctgcttg tcgggatcgg
     8761 gctcgtgacc gcgcttggca tgatgggcct tgccggcatt gggctgaaca tggcggtgat
     8821 cggggcattc ccggtcctga tcgggctcgg catcgattac gcgatccagt tccatgcaag
     8881 attcgatgag gaggcacgga aagggtcgct cgaggacgcc gtcttcacga ccgtgaccaa
     8941 gaccggcccc gcggtcctct atgcgatgct cgcgacctgc atgggatttg tggcgatgtt
     9001 catctcagat gttccgatga taagggcctt tggcctcgtg gccatcattg gtatcttcac
     9061 ctcatactgg gcttcctgca tcggggtgcc aacgctctgc ctcctgttga agtacaggcc
     9121 gaaggagcca aaaacagaga cctgctacgc ggtcggaacc gacgcatgcg atttcgtcat
     9181 ggattcccgc aaaaatagga acgggaagaa aaagtccttc tcttacaggg aattcctgac
     9241 gggcgtctcg gtaaaaatag ccagaaaccc cctgcccatc ctgctggtgc tcgggatcat
     9301 cgccggtgtc ggttttgcga tcgacccctc catccctatc cagacgaacg aggactcgtt
     9361 cgtccccggc gatatgccgg caaagatcaa tatcgacaag gtcacccgca tcatcgggtc
     9421 gacgagcacc gcggatttct atgtcagggg gtacgggacc gatctcgata ccatccgctg
     9481 gatccagaag ttccaggact atgaactctc ccatcatcca gagctgacag gagcacagag
     9541 catcgtctca gcggtcctct cgtatcatgg aggttctctg ccggagaccc aggcggaact
     9601 cgacgaggtg ctcgcttccc tgccggaagg gaccagggac gagtacctgg gggggtcact
     9661 ttccggtgtg gtgaaattca gcaccatcga cctggatatg acgcagcggg actcgttgaa
     9721 gcagcagatg atcgatgaca tcaccttcct cgaacctccc gcaggaatca gcgtggctcc
     9781 gataggcagt tttgatctct acacgatgct gctgtcatca ctggcagact ccaaggaagc
     9841 catgacctac cttggattca tctttgtctt catcttcctg gtcctcgtct atcgtcatct
     9901 ccatgcaatc accccgatga tcccgatcat cttcatagtc ggatggaatg ccgtggccat
     9961 gtatctcctg gggattgcct ataccccgtt gacggcgaca cttgggtcaa tgacgatagg
    10021 ggtcgcagcc gaatacacca tccttgtcat ggaacggtac gccgaggaga aggaacgcct
    10081 tggtgacaat ctggcagcaa tccaggagag tgtccacaag atcggcacgg ccatcacggt
    10141 ctctggcctt gcgaccttct gcggattctc ggcactctgc cttgccacgt ttcccatcgt
    10201 cagcaatttc gggtacacga cactgatcgc ggtcgggttc tccctctgcg gggcaatcgt
    10261 tgtcatgccg gcggtcctct cggtgatggg aaggtttgaa gaccggttcc atcatcaggt
    10321 cgaagaccgt tcctctgctc cggagtcgtg aagagaaggg gtgggattgc aggggacctc
    10381 tttttctcct cataccgcca cgcgtggaac ctggaataca gggtatattg tggatatccg
    10441 ggcattctgt atcctgaaga tggtgaaaaa gaaaaccagg atcccgcacg gcgttcctgc
    10501 atatcggccg ggaggggagg tatcagggag gacaaccgaa ttctggtccg gaaaaccgga
    10561 ttactccttc ctcccaatca ccatctcgca gtagggatcg ccccggcacc tggccttggt
    10621 gacggtgatg gtgtatcctg gattgagacc ctggatcgca tgctccacgt atgcctggca
    10681 gatgctgctc acctggtgat gcgtgacatc acgcatacgc tcctcgcgga acattgcgca
    10741 gccggtcagc cgtatcactg cctcatcggg aaatccttcg atataccggg tctcgaactc
    10801 cggtccgaac aagatgaccg agatggcccc aagggtctgg acaaggtcgg acgcgttctc
    10861 ccgtggcatc ccgtaccggt ctgcaatggc acggatctcg cgggacatgg ctgagaacat
    10921 gctgttccga aaggagggaa cttttgtgcc gctcaccggt tcatagccga gggccactac
    10981 cagcctggtg agcacgtttg tggccatcgt ccagcgggtg gcaatcggga tgtcttttac
    11041 tcgtatcatg ctgattcccc gtaataatga gagtccgtgt cggtcggact ggtatccaga
    11101 actttctctc tgcactataa agcattttcc aggcgcttgt catggtgccc ggatagtgat
    11161 gaacatcacc gtcctcaccg caggaaccct gccatcaggc aatggtgatg ccgttcccgg
    11221 ggaagttgac accgtgaata ctgtggcggt tgcatgcaag catacggata aagagggata
    11281 cggggctccc tttctgcatc acccgggaaa accgggtaac aaagcctata tcggtaaaga
    11341 acccaccagg attgaggaat agacaactcc acctatacca tgatccctgc cggactcatg
    11401 ctgccgctta ccatcttcat tgcacggata gtcgagacca gtctcgagac gatccgcacg
    11461 gtctatgttg cacgggggca caagtacctc gcagccggga tcggggtggg aaagatcgca
    11521 atatggctcc tctccaccgg gctggtgctg acaaacctgg acaatatcgg gggcattatc
    11581 gcctatattg ccgggtatgg cattgggacc gtgattggca tggagatcga ggaccggatc
    11641 agcctcgggg atgtcgtggt ccggatcatc agcgcaaagg atccccagcc tctcatcacc
    11701 cggctttcca gtatgggata cgggatcacc cggctcgatg ggacgggcgt attctcgtcg
    11761 tctgttgccg tgctgttgat ggtggtaccg agaaaaaatc tcgagcagct gaccgccctg
    11821 ctcagaaaag actaccctga tctgctctac acggtcgagg atgtccggaa ggccagtgag
    11881 gggggaacga tcttccatgg gaaggagtct ggctggatct cccggttctt cggatggtga
    11941 cctcccgttt tccctaccct cctgcttgag aaaaattgtc atgagcgggg cttctcaccg
    12001 ttatgaatga ttccgtgcat gagtactaat cggaaataga gaggatccag gggagtcttc
    12061 cttcactccc gcagggtgac catgattcgc tcccgggaga ggaacctggt gagccaggcg
    12121 gtagccagga tgatcagcaa ggccagccct gccgttgccg ccaccacccc ccacgtcact
    12181 gaaaatcccg ggcctgcaag ctcacggacg aacgtgatca ggaatatgat gccaaagatg
    12241 atgccaaagt tgaggatctg gttctctctc atgccaagca tgaactgcgt gaagcctatc
    12301 aggccgctcg tcgcagcgat aaatgccggg accacgataa tgatatggaa gaggaccggg
    12361 cccgagggga agagggtttc cccggtgaag atggaagcgg ttcctgtgat aagtgctgcc
    12421 gagaagtagg tcaggagggt tgccgggatc gtgaccccgg ctactttacc agaccagagt
    12481 tcccgcaggg agagaggggt acaaagcagg gtctcgatca caccggactg cttctcccgg
    12541 agaaaaacct gtgcggtgaa gagatagccc ataaagatac ccagggtgag cacaaggtaa
    12601 aagccgaggg tatccatcgc attcccggcc tctcccatgc ccccaagcgc cgccgggatg
    12661 gtgaatatcc cgaacaccac cacaaagatc aacgcagaga gcatgacgtt gcgagatttg
    12721 aacagctggc gtacctcttt ttctgccacc acggtaatcc tgctcatgcc acccctcctt
    12781 ctgcctgtcg catcacgtca aggtagatct cctcgagtga tctcttctgg atccttgcct
    12841 cctcaacctg tatgccgttt cttacgagag cagccacaat ctcgtgggga gggacggtca
    12901 caagcagtat cccaaggaca ttctcattcc gggcgacatc gtcgactcct tccactgagg
    12961 agaggatgtc cattgcctta cctgcctggc ccggatcagc aagagtcact gaaaacccag
    13021 tcttcccccg tgccgaggtg aggtgccgga cgctgtcgag ggcccggata cggccctggt
    13081 gcagtattgc cacacgggaa caggtccgct gcacttcgtc aaggttgtgg gagtttaaaa
    13141 aaacggtcat cgattctgcc tgggagagcc gaaggatgag ctcccttacc atgtgctggg
    13201 cttcgggatc gagccccgag gtgggttcat cgaggaagag gatctccggc cggtgcagga
    13261 gagcccgggc aatccccagt ttacgtttca tcccggtgga gaaattcccg acgaggtcct
    13321 tttcccggcc ggtcagtccc acgaactcca ggagctcggt tacccggtct gccggccgga
    13381 tcacctcata caggcgtgca taatacacca ggttctccct ggcagtgagc tggtcgtaga
    13441 ggccgttgtt ctccatcagg acccccaccc ggttcctcgt tgcgtcgtct tttgcgagat
    13501 cggcatccag caccgttgcc gaaccggatg tgggtgacag cagcccgagg agtactcgca
    13561 gggtggtggt cttgccggcc ccgttcggac caaggtaccc gaatatctct cccttctcca
    13621 cggagaatgt tatctcgtca agtattttcc ttcccgaaaa attcttggac aggaactcta
    13681 cgtcaattac cgccagggac gctcacccct ttctccggga agagcgtgtc ctttcggtgg
    13741 tgcccggctc cggatctgat tgtgttgata gagcgcatcg tgatatcccc gatatacgtc
    13801 agcaggacgg gagaggaaga tggaaggggc atgcgttttc gaatgaaaat gcctccggta
    13861 ttttcatacg ttctgctcta atcgatagcg ttcatcgact gttcgaatga aatgctcgca
    13921 ttttcatctt ttatccttga ccggacgacg gccatcggct gttgatatga caccgaacca
    13981 ggcattcttc atctggtctg cctgcgtcta cggagggaat catgccattt tatcaggaca
    14041 tgcccctggg agagagagcc ttctgtcccc ttcctggtcc cgtcccagtc atggttcaat
    14101 agattgccct gctggcggta ttattttatt ggattggctc tcccactttt ttgcgatcag
    14161 tacgttcatg cctgatatcc cgcaaaatac cggcctggtt tccttggaga ttctcattct
    14221 tatcctgctc cctgcggtgc ttctctcagg gtgtatggct ccctcccctg cagtggacga
    14281 ttctggtcct gtgactgaaa ttccttcagg tgctgcacca ggtcctgctg gtgataattg
    14341 ccgtctggat caccttgccg gtcgtggcga tgaatacctt tcgaacgggg attcctgcta
    14401 ttttggcacc caccgtcccc tggattttct ggatgacctt cggatgcgtc cccaccagcc
    14461 ggtcatggtc cttactgtcc ccgatggctg gatcacgcgt gatgacgcag agctgctgat
    14521 gcaggagatc gattcagaag aatctgcagc accggtggtg tccccgctca gctcctactg
    14581 gccgtatgac cagacctcaa cggtggggaa cgaggcactc ttcctcttag aggggtaccg
    14641 gaccggaagg tatcccccca ggctctgttc cctgtattat ttcaaaccgg acaggtccga
    14701 ggtccggtcg tggtgggaga cctatgggcg aacaggcagc atcgatgaac gagacgcgat
    14761 caggaaactc caggagacat accctgatct ctctgcatac tcatcagagg gtatgccacc
    14821 ccgatcgata aggaccgaac aggccgacgg gggttggtac gttgcgttca tccaggaagg
    14881 ctccgggttc cccatcctct cggcccggtg ttattatgtg gacaacaatg gatcgacccg
    14941 cctcacgagg tttgtcaacc agagcatcat ggtattaccg gaggatctct ctccgagacg
    15001 gtgcggctga tcgcactgat ggtctggagc cggactcctg ccggaccggc aggtccttca
    15061 tgagtggaaa gggagaacag ctgccggcac agagtaaaac agcagagccg ggagcgtctc
    15121 gatccattgt cagtcctggg aatacaaggg aggtgaggag agatgaacat ccgaccagtc
    15181 tctgtttcac ataatccttc ccgttataac cacctttttt taggatcttt acctaatgat
    15241 gcataaaacg cgaggaagac ggtacctctc tgcgcaaaag cccggatgcg gtatctttca
    15301 ccagctctcc tgaggtagga acgacggatg caaccctcca ttgaatacat cctccttgtc
    15361 atcgcggtac tgatcctggt cagtatcctc gccaacaagg tgtccggaag gcttggcgtt
    15421 cccgctctcc tgatctttct tctggtaggg atgcttgccg ggtcggaggg tcccgggggt
    15481 atctactttg acgatccctg gatagcccag gctgtcgggg tcattgccct cacctacatc
    15541 cttttctccg gagggctcga tacccgctgg tctgaagtca ggccggtact tccccaggcg
    15601 gcaatcctct cgacattcgg ggtgctgctc accgcgcttc tcatggcagg tcttgcgatt
    15661 gtcgtgttcg aagtctcgct gctcgaaggg gtcctcctgg gagccgtcgt atcatcaacc
    15721 gatgcggcag cggtcttttc tattttacgg acaaagaggg cgagcctgaa gggaatgctc
    15781 cggccgctcc tcgaactcga gtctgcaagc aacgatccga tggcggtctt cctcaccatc
    15841 ggggccatca ccctcctcct gaatccaaac ctgtcccccc tcatcctgat cccgctcttt
    15901 atccagcaaa tggcggtggg tggcctcatc ggatacggta tgggcaaagc gatggtctat
    15961 ctcatcaatc ggctcaagct cgagtttgaa ggcctctatc cggtgctcac cctggccctt
    16021 gtcatgatga cctacggggt gaccgcatcc cttggtggaa gcggatttct cgcagcatac
    16081 attgcagggt tggtcctcgg caacagcatg tttatccata aaaacagcct gatgcggttc
    16141 cacgagggga ttgcctggct gatgcagatc gcaatgttcc tcaccctggg cctgctggta
    16201 tttccctccc atctcattcc ggtcatcggc tcagggattt tactctccct cttcctcatc
    16261 tttgtcgccc gcccggtcac cgtctttctc accctcagtc cctggaagat ggcgcttcgg
    16321 gacaaggtca tggtatcctg ggttggactc cggggagcag cccccatcgt ccttgccacc
    16381 ttcccgctcc tggcaggcat accccaggct gaaacgatct tcaacctggt attctttctc
    16441 gttatcacct ccgccctcct gcatggaaca tcgattcctt tcgttgcccg gcatcttgga
    16501 gtctcagatc ccctgcatga gacaccccgt ctgtccatga gccttggaga cggatgctca
    16561 tcgggcaacg atctgatcga gctgactgtg ccaatccagt ccggggtggt gggtatgcag
    16621 atcgtggata tcgggctgcc tcccgggacc ctcatcatca tgatcgagaa agcgggaaac
    16681 cggttcgtgc cgtgtggcag taccgtcctg gaaggaggtg atcacctgct cgttcttacg
    16741 aatcagccac aatcgaacca ggttcgctcg atcttcatcc ccgaaccatt tgcggagaac
    16801 acgggtccgt aaccggatca gggtgcgt
//
LOCUS       DAOOCO010000016        16247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3225_length_16247_cov_5.864872, whole genome shotgun sequence.
ACCESSION   DAOOCO010000016 DAOOCO010000000
VERSION     DAOOCO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_16247_cov_5.864872"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1245
                     /locus_tag="PK154_01845"
     CDS             79..1245
                     /locus_tag="PK154_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPW09836.1"
                     /translation="MDLKEVVLNYLTDNDEGMKHLITWFLNEVMQEEANQQTGAGRYR
                     RTSSRKAYRNGQRKRTLKTRHGELILNKPQLRDIPFQTKVFERYSRTEKALESAILES
                     YLQGVSTRKIQEIVAHLGVEKISASYVSKIATELDQNVHLFLSRPIETYIPYLFVDAS
                     YFKIRDGIRYTNKALLVIAGIRSDGFREILGARIADCEDELTWEDLFSELKERGVGKV
                     DLVISDGHKGIQAAAERVFTGSSWQMCHVHFIRAVLRKLPKKQHKEIAQMLKDSLSDP
                     RRLQECADELEIRGFPRAADTVERFIPGLLNYRIAPPDHWRRIRTTNMLERLNKELKR
                     RSRTIGAFPNDASLLRLAGTILMDINEEWITSRRYLSGSDVIVCQDTRAEFPAV"
     gene            complement(1591..2388)
                     /locus_tag="PK154_01850"
     CDS             complement(1591..2388)
                     /locus_tag="PK154_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HPW09837.1"
                     /translation="MEMKYLGRDDAPIKAETWNLLDQTMVEAAKSILAGRRLLSIEGP
                     YGLGLKGVPLEDCSVGECLLVSKFVPVHMISTTFPLGVRDLIAYEQNGLPFATSQLID
                     AAIKCAKMEDSIIFEGVAGASGLLNAEGVNGQKLASWNTVGKAADDIIQGMITLDEAG
                     YHGPYALALAPSRYNLLFRRYLQGIGTELEHIRTIVTGGVFKAPALKSGGVLLATGKQ
                     YASIVLGQDMTTGFVGPVGDSLEFSISESLALLVREPASICVLREKA"
     gene            complement(2395..2769)
                     /locus_tag="PK154_01855"
     CDS             complement(2395..2769)
                     /locus_tag="PK154_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW09838.1"
                     /translation="MAEFANAFYGNVSPRKLTHSELVRAIRLMITDEYEAISIYTQLA
                     ESIDNELAKEVLIDIANEERVHAGEFLRLLKELAPDEEQWYADGAAEVEAEIAKMAGA
                     PAGKGSAEGGDTKPLTIGSLKE"
     gene            complement(2870..3598)
                     /locus_tag="PK154_01860"
     CDS             complement(2870..3598)
                     /locus_tag="PK154_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09839.1"
                     /translation="MDIIYLLKDFIHSKNVQSWINNRYKDKNKVIVDPNIFPFVMMSK
                     GEIFSTLSNLQIIAQVFRDYKFSDIRKEDIVIDIGANIGGFSFPASKISNKVFAIEPI
                     EIEQLNKNNKINGMNVNIIEGALGDGLEHTIKWLNTSQKIRTLSFTEIKKRCGGCDFL
                     KCDCEGFEWFIQPDELKGVRRIEMELHNYNPSNNNPNLLINHIFKNYNTIIQNEREYE
                     ITEFSMKFNKTKITEVMILHAFEK"
     gene            complement(3615..4256)
                     /locus_tag="PK154_01865"
     CDS             complement(3615..4256)
                     /locus_tag="PK154_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPW09840.1"
                     /translation="MIPGVEEELAKLTTLAAEKGVKTKKISASDVIVSDWVRFKCRFG
                     CKGYAKHFGCPPYAPSPHDTRAMVGEYQTGLLLRFDGVPGHESFGPDDLPEDFHHFYK
                     DLILWVNTTVHMIEKTAFYDGFYKAFGFGGYPCIFCEECVAEQCEGVVDESMRRKCRH
                     MDLVRPSMESAGIDVFATAKNAGWELSTIPCRDMEYGKVIHSNIVSIGLILID"
     gene            complement(4323..5801)
                     /locus_tag="PK154_01870"
     CDS             complement(4323..5801)
                     /locus_tag="PK154_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPW09841.1"
                     /translation="MTEKPALFQQFMNIEAVRRQSLIHIGTNIVITAVGFLSTFYFAH
                     VLGPSILGAYFLFLAYFGLFNLIVDGGLGNAAAKRMSEGKDQEEFYSAAAVLRLILLA
                     LSLVILFLLRPYLVDLSASDLFWWLVIALIVNLPFGIVAGGNYGLGKAGVVQGSNLAN
                     NIARIILQVIFVFFGLQIAGLAGGFIAGLAIGFLFNYHYLEVKFRTFSRAHLKSLFTF
                     SFWIFLAMSGILILTNIDVVLIGYFMGNSEVGVYRVALQLASLGVFVALALEFVLFPR
                     FSGWNEEGSYDIIGTALSRAYTYSLVLAVPFCVGGWLLGDKLLYYFYGEAFTTGTVAL
                     MVLLPLYVIYVFQYLQMMTLNSLNHPKDSFWITSAIVVVNIVLSVLLIPVMGITGAAF
                     ATLIAILISSVLGYVVLGKYIPMKIEKEPILHILAASGIMALVVGGMRFLFPVESLAY
                     LIVLVILGSLVYFVLLLRIDRDIHDELKAISGHVGIFWPDFL"
     gene            6128..7294
                     /locus_tag="PK154_01875"
     CDS             6128..7294
                     /locus_tag="PK154_01875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09842.1"
                     /translation="MKPKLRVVLVGPSIRFLSGISYYTIRLANAISESTDVTAILFRN
                     MLPRRIFPGWKRVGDPLSTVSYTAEVKVMELLDWYSPISWMRAAAQLRKADIIIFQWW
                     TSSVAHMYVALQLMRFRKVPIIIEYHEVVDPLEHSILPIRIYSVLMGRIIRKRADGYV
                     VHSEADRALVETSYHVPQDKVMVIPHGLYDQYPVIERTIARKQLEIAEEHVILFFGLL
                     RPYKGVKNLIEAFDSLPTELLHSTRLLIVGEAWEDKDSLARAEKSPNRNKITIVDRYI
                     GDDEVPVYFSASDIVALPYTRASQSGVAHIAMAFGLSIIATKVGGLEEGLQTYSGALF
                     ISPNNKEELKDALIRQIGKKDSFPPPANLAWSGIGKQWTTFLSSVILNRSNEGA"
     gene            7296..8342
                     /locus_tag="PK154_01880"
     CDS             7296..8342
                     /locus_tag="PK154_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW09843.1"
                     /translation="MKIIQVCPRYFPDIGGVETHVREIAERLVKKGHKVEVVCTDPCG
                     VHPKNDFINGVSVTRFRSFAPHYAYFFAPKMAPYLKTLDAEILHAHGYHAFPALFAMM
                     AARNKRFIFTPHYHGKGHTPLRNMLLTFYNPLGRAIFNRANRIISVSEYEKNLIIGKF
                     CIPEERFSVIPNGLNLSEFKGIIGAKDPHRILHVGRIEQYKGIQHVIGALPELPEFTF
                     TIIGKGPYEHSLQQLAADKGVSDRVTWKKDLSRNELLQEYASAELFISLSSFEAYGIT
                     VAEALASGLQVIVNKNGALEEFVDGTTCIGIEPSVKDVIGAIRLLKPCQGYQKRIMDW
                     NEVVERIIETYSSG"
     gene            8426..9445
                     /locus_tag="PK154_01885"
     CDS             8426..9445
                     /locus_tag="PK154_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPW09844.1"
                     /translation="MKVLHWNISKKNQPLSGVKRYEDELFTNIRKIDPSLEIDRIHRL
                     ENGFLGSTPLSWLYRYAVKDADIVHATYQTLAPSIRIKKPKKFIVTVHDLAPLVYPEL
                     QDNFSTKIQWQMTPRGLRKADFIIAISNFTKKEIVRLCKIDEQKVEVVHQGVDSERYH
                     PLDKDKSRKEIGLNPEGEYILVVASNLTHKRMDLTKKIFDNVRKQRPDVQLIKVGYGD
                     VLQGQGIINLGWVKEDDMPLLYNAADLFLHTAEYEGFGLPILEAMACGCSVIGMNRAS
                     IPEIIENSGILMNTDRVNEWTRAILDLFENDELRRDLHRRGMRRGELFSWEKTAHETL
                     SIYNS"
     gene            complement(9487..10536)
                     /locus_tag="PK154_01890"
     CDS             complement(9487..10536)
                     /locus_tag="PK154_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09845.1"
                     /translation="MDKCSLDFVTSPPKVCGIGEYSDHIIEELKKYCEIHPVYIEPHK
                     NPFYYIKKADECSRKLIHVQFEYGFFWGIKIIHGIYAWLFYPIIARDRYVITTFHEIL
                     HKSIPDALILRLISFYSKKVIVHTTEAKERLLQAGVKEDKIEIIPHGVLYTAFPLDKK
                     NAKRELNIEADEKTVLFFGFLHRDKGVDNLIEAMKGIDATLIIAGDIHPFNKGKDGWY
                     LENLHFLANDPAVKVKFLGYVSDEKIPLVMSAADIAVLPYKKILQSGVFNIISAYKIP
                     CLASKIRFFDDTNRQYHYPYIYTNLHDDIVRLFRDFELRNQLTKFAENYNADNSIENV
                     VRKTVDIYKKILEIR"
     gene            complement(10544..11638)
                     /locus_tag="PK154_01895"
     CDS             complement(10544..11638)
                     /locus_tag="PK154_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW09846.1"
                     /translation="MGKLKKQIEGCILIKPTVAIIILNWNGWRDTLECLESLYQIQYP
                     NYTVVLIDNGSVDDSLEKILAYCNGDFKVESLFFEYKTQNKPIKIFEFSREELKFNEW
                     KKNDIENISSDRKLILIKNEKNYGFAEGNNIGIQFSANFLNPKYFLLLNNDTVVEKSF
                     LNALITDMDQDSNIGFIGPKIYYYTLNNRMDILSHCGGEIDIWRGETHHIGDKEIDKG
                     QYDEVGNIDYITGACLLVTSSLIKKIGLLNPSYFLYWEETDWCLRGKRAGYKIKYEPR
                     AVIWHKGSQSGTNSTFEYYNTRNRIWFEKDNAPVVPFIIFFIYFMGYKFWRTLFSRLF
                     FNDPKLVIPLVKGVKDGFFMPHSNMSRSDH"
     gene            complement(11699..12964)
                     /locus_tag="PK154_01900"
     CDS             complement(11699..12964)
                     /locus_tag="PK154_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW09847.1"
                     /translation="MNQDILESQNPVFLTYFPECKNIHLTKDVGMIPFILHRSFSYNS
                     YILTYRNDEYSYLNVETPGLKLCFINKSREKKGASNKIIDNSPDNSYPKSFFLRILFA
                     WRFLLKNRNKITVFQLYHYKWESRLVALLYRLYNPKGILYLKLDMDPDSIETLRRKLK
                     KMKWRIFYQYIILKLTNFDIISVETEIALNFLKKEHPLFTKVADCLHYVPNGFDSQNP
                     VVRELLERPPAREDIIVHIGRLGAYAKGTDVALESFASIAPSFPHWKLVLIGPMEDSF
                     RKYFADFLESHANIGPQINYLGYLPNRKDIFREYAKAKILLQPSRFESFGLVVTESGI
                     LGTILIGSNIGSFKELTNDGRYGVLCPVDDIQCFSKALQNILSHPDVFDGMSYLLKSH
                     MQKRYNWNDICNELNDIIFSCIEKKIDGV"
     gene            13066..13773
                     /locus_tag="PK154_01905"
     CDS             13066..13773
                     /locus_tag="PK154_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HPW09848.1"
                     /translation="MTPPPSGCLICGADLEYLDQPEEMVCQYCKRVCKSTTRCVNGHY
                     ICDRCHRLDTEDLIEVICLQSTEDNPFTLIERIFLSPTVMMHGPEHHFLVPAVLVAAF
                     YNHTQQPGKKELGIKNARHRAGLVKGGSCGLLGDCGAAVGAGIFVSIVTGATPLSREE
                     WRMANLMTARALEKIALHGGPRCCKRNTFLALQEAIRFISTEMGIHLPEPRDISCRFS
                     DQNRECLKDRCPFYNGI"
     gene            complement(13945..14370)
                     /locus_tag="PK154_01910"
     CDS             complement(13945..14370)
                     /locus_tag="PK154_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HPW09849.1"
                     /translation="MKTVVATGTFDLLHPGHLYYLEESRRLGDELYVIIARDANVRHK
                     PRPVIPEDQRMKMVGALKPVDHAVLGDLHDMFRPIREIQPAIITIGFNQHFDEAKLVA
                     DLRSRGIGSAVVRIDQYPGDSCSSTSRIIRKVLEQYREE"
     gene            complement(14367..14738)
                     /locus_tag="PK154_01915"
     CDS             complement(14367..14738)
                     /locus_tag="PK154_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HPW09850.1"
                     /translation="MRIRGIRKDLLSLLLQLGREQHPNEYAGLLREEEGIIEEINLLP
                     GTTSTDRSASLFLDMMPLDTHLAGSVHSHPNGVIRPSDADLAFFPRTGRYHIIVGFPY
                     EVDDWRCFSANGMQVDIGVID"
     gene            complement(14797..16209)
                     /locus_tag="PK154_01920"
     CDS             complement(14797..16209)
                     /locus_tag="PK154_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HPW09851.1"
                     /translation="MRILLPTGSATAGMVRKAAEGMDAVVPVTGEIASFLTPAQLEEL
                     IGEGKYDLVLVSGMCTASFHGVEQRTGVPIYRGPRHAADLALILPLLGSISLSRTIAA
                     DDLLAIERRDEAVRTIARKEREAESEYLIRGVKIGGNSRMKVLAEIMDAHKEPDIRAR
                     ALSCFSSGADIVDLGFGFDATPGDVKRVFSELSDLPGPLAIDTQDPVLIAAGLPRADL
                     VLSLSDRNIALVGRRVAECGTGAVVVPGERSIEENIRLAEEAGISCIIADPLLAPVGS
                     GLIASLSHFTDPGYPLFFGAGNVAELIDADSPGVNALLAGMAMEVKASVIFTSEHSDK
                     TRGSVREMRRATEMMVLAAERPYPKDLGIDLLILKEKRRRREPPLEYCEEVRAETMPK
                     EITYDPCGNFRIGIEGEEIVAVIGGKAYRGRSWAGVFHAIQECGEVSLLDHAAYLGAE
                     LYKAELAIRFGRSFEQDGPF"
BASE COUNT         4493 a         3839 c         3602 g         4313 t
ORIGIN      
        1 ggtagtgtac acgaagtgat gtaaaattgg aaaaggccct gtatcctact cagtttaggc
       61 aacaggagaa tacagggcat ggatctaaag gaagtcgtac taaattatct taccgataac
      121 gacgaaggaa tgaagcacct catcacctgg tttttaaatg aggtgatgca agaggaagct
      181 aatcagcaga ccggagccgg gagataccgg aggaccagtt caaggaaggc ataccggaac
      241 ggccagagga agaggacgct caagacacgg catggcgagc taatcctaaa taaaccgcaa
      301 ttacgcgata ttcctttcca gacgaaagtc tttgaacgat attcccgaac cgagaaggcc
      361 ttggaaagtg ctattctgga gtcgtatctc cagggagtct caacccggaa gattcaggaa
      421 attgtagctc atctcggtgt cgaaaagatc tccgcttcat acgtctcgaa gattgccact
      481 gagctggacc agaacgtcca tttgttccta tcccggccaa ttgagaccta tatcccgtat
      541 ctcttcgtcg atgcctccta tttcaagata cgagatggaa tacggtatac caacaaggca
      601 ctcctcgtca ttgccgggat ccggagtgac ggattccggg agatcctggg tgccagaatt
      661 gccgattgcg aggatgagct aacctgggaa gacctcttct ccgagctgaa ggagagagga
      721 gtggggaagg tcgacctggt aatctctgac ggccataaag gaatacaggc agccgctgaa
      781 cgtgtcttca cggggtccag ctggcagatg tgtcatgtcc acttcattcg agcggttctc
      841 cggaaactcc cgaagaagca acataaagag atcgcccaga tgctcaagga ctcactctcc
      901 gatccacgga gactccaaga gtgcgctgat gaactcgaga ttcgaggatt tcctcgtgca
      961 gccgataccg tggaacgatt catcccagga ctgctgaatt accggatcgc tcccccagat
     1021 cactggagga ggataagaac cacgaatatg ttggaacggc tcaataaaga gttgaaacga
     1081 agatccagaa ccattggagc gttccccaat gatgcctcat tgctccgtct tgcgggaaca
     1141 atcctcatgg atattaacga ggagtggatc acgagtcgta ggtatttatc tgggtctgat
     1201 gtgatagtgt gtcaggatac tcgggcggaa tttccagcag tttagatata ctgcccagat
     1261 ggagcgatcc cttttttacc ggcatccacg gtatctgatg atgagtcatc cgaaaaatga
     1321 agacagcccc gggtctttca gccagtatac tcagagattt cctgggctcg agattcaagt
     1381 aacactttta aaaaaatcac ctgatcgccg tgcatccatg caggatgaag gtgcaggagg
     1441 cactttcgta ggaaccgcct tagagcgatg tccgacctag aatagagggt gaacctgcgg
     1501 gagcgttctg gtaaatcgca tccgcgattt tcatcaggaa ggcatctgac ctatcggtat
     1561 aataggcgtt tcattcgaaa aaaaaaggat ttacgccttt tccctgagca cacagattga
     1621 ggcgggctcc ctcacgagaa gggcaagact ctcggagatg gagaactcga ggctgtctcc
     1681 gaccggcccg acaaatcctg tggtcatatc ctggccgagg acgatcgatg catactgttt
     1741 cccggtcgcg agaagaaccc cgccgctctt cagcgccggg gctttaaaga ctcccccggt
     1801 cacgatggtc ctgatgtgct caagctcggt tccgatgccc tggaggtagc ggcggaagag
     1861 caggttgtag cgggatggcg cgagggccag ggcgtagggg ccgtgatacc cggcctcgtc
     1921 cagggtgatc attccctgga tgatatcgtc cgctgccttt cccacggtat tccatgaagc
     1981 cagtttctgc ccgttcaccc cctctgcatt cagaaggcct gaagccccgg caactccctc
     2041 aaagatgatc gaatcttcca tcttagcgca tttgattgct gcatcgatca gctgggatgt
     2101 tgcgaacgga agtccgttct gctcgtaggc gatcaggtcg cgcactccga gaggaaaggt
     2161 ggtgctgatc atgtggaccg gcacgaattt actcacaaga aggcactctc cgaccgaaca
     2221 gtcctcgagc gggacccctt tcaggccgag gccgtagggc ccctctatgg agaggagcct
     2281 tctcccggca aggatgctct tggctgcctc caccatggtc tgatcgagca ggttccacgt
     2341 ctccgccttt atcggcgcat cgtctcttcc aaggtatttc atctccattg tctctcactc
     2401 cttcaatgac ccgatggtca aaggttttgt atctccacct tcagccgacc cctttcccgc
     2461 aggggcgcca gccatctttg cgatctctgc ctctacctcc gctgccccgt ccgcatacca
     2521 ttgctcctca tccggggcaa gttcttttaa cagcctgaga aactctcccg catgtactcg
     2581 ctcttcgttt gcgatatcga tcaggacttc ctttgcgagt tcgttatcaa tcgattctgc
     2641 gagctgggtg tagatcgaga tcgcttcata ctcatcggtt atcatcaacc ggatcgcacg
     2701 cacgagctca ctgtgcgtga gcttccgggg actgacgttt ccataaaatg cattggcaaa
     2761 ttcagccatg taaatcactc cgtagagtat cacaagggca tggataccct tctacgccta
     2821 ctttcatttt aattgatata tatgtatgga agatatcgtt acaatggatt catttttcga
     2881 acgcatgaag gatcataact tctgtaattt tagttttatt aaatttcata gaaaattcgg
     2941 ttatctcata ttccctttca ttctgaatta tagtattata attcttaaaa atatgattaa
     3001 ttaacaaatt tggattgtta tttgatgggt tataattatg taattccatt tcgattctcc
     3061 gaacgccttt tagttcatcg ggttgtataa accattcaaa accttcacaa tcacatttta
     3121 aaaagtcaca tcctccgcac ctttttttaa tttctgtgaa tgaaagcgtc ctaatttttt
     3181 gtgatgtatt taaccatttt attgtgtgtt caagcccatc acccagtgcc ccctcgatta
     3241 tattaacatt cataccatta attttattat tcttattcaa ttgttcgatt tctatcggtt
     3301 caatagcaaa taccttattt gaaatttttg atgcaggaaa tgagaaacca ccaatgtttg
     3361 ccccaatatc aattactata tcttcttttc taatatctga aaatttataa tcccgaaaaa
     3421 cttgggcaat tatttggaga tttgataatg tagaaaatat ttcacccttt gacatcatta
     3481 caaagggaaa tatgttagga tcaacgatta ccttattctt atctttatat cgattattta
     3541 tccatgattg aacattcttg gaatgtataa aatctttcag taaatatata atatccataa
     3601 gttgttcttt ccccttaatc tattaaaata agccctatcg atacaatatt gctgtggatc
     3661 acttttccat actccatatc cctgcatggg atagtggaga gctcccatcc agcgtttttt
     3721 gccgtggcga acacatcaat ccccgccgat tccatgctcg gccggacaag gtccatgtgc
     3781 cggcatttcc tccgcatgct ctcgtcaacc actccctcgc attgctcagc aacacattcc
     3841 tcgcagaaga tgcaggggta tcctccgaac ccaaaggctt tgtaaaatcc gtcataaaag
     3901 gctgtttttt ctatcatgtg gaccgtagtg tttacccata ggatgagatc cttgtagaag
     3961 tggtggaaat cctcgggaag atcgtccggt ccgaatgact catgaccggg aaccccgtcg
     4021 aaccggagga gaaggccagt ctggtattca ccgaccattg cccgtgtatc atgaggagaa
     4081 ggagcatacg gggggcagcc aaagtgttta gcatatccct tgcaaccgaa tcggcacttg
     4141 aaccgcaccc agtccgagac gatgacatct gatgcagaaa ttttttttgt ttttacccct
     4201 ttttcggctg ctaaggtagt aagctttgcc agttcctctt cgacaccggg gatcatggat
     4261 tcctctccat tgtggaaaat ggattcgagg ggaatataga ttttccgggc atacggacca
     4321 ggctatagaa aatcaggcca gaagatacct acgtggcctg atattgcttt cagttcgtca
     4381 tggatatccc tgtcaatcct gagaaggaga acaaaataga ccaatgatcc caggataacc
     4441 agcacaataa gatatgccag gctttcaaca gggaacagaa accgcatccc accgactaca
     4501 agggccatta ttccactcgc tgcaaggatg tgaaggattg gttctttctc gattttcata
     4561 gggatatatt tcccgagaac gacgtatccg agaacggaac ttatcagaat ggcaatcagc
     4621 gtagcaaagg ctgctccggt tatccccata actggtatca ggagaacgct cagcacgata
     4681 ttcaccacaa cgatggctga agtgatccag aacgaatctt tcggatggtt gagagagttt
     4741 aaggtcatca tctggaggta ctggaataca tagatgacat agagggggag gaggaccatg
     4801 agggcgacag tgccagtggt gaatgcttct ccataaaaat aatagaggag tttatcgcct
     4861 agtaaccagc caccgacaca gaaggggacg gccaatacca gggagtatgt gtacgcccgc
     4921 gagagggcag ttcctatgat gtcatagctt ccttcttcgt tccagccact gaatcttgga
     4981 aaaaggacaa attccagagc gagggcgaca aataccccaa gagatgccag ttggagagcg
     5041 acacgataaa caccgacctc ggagttgccc atgaaatatc cgatgagcac aacgtcaata
     5101 tttgtcaaga taagaatccc gctcatggca agaaagatcc agaacgagaa ggtgaacagg
     5161 ctctttaagt gagccctgga aaacgttcgg aacttcactt caagatagtg gtaattaaaa
     5221 aggaacccga ttgcaagtcc tgcaatgaaa cccccagcaa ggccagcaat ctgcaggcca
     5281 aaaaagacga agatcacctg gaggatgatc ctagctatat tgttggcgag gttacttccc
     5341 tgaaccacgc ctgcctttcc gagaccgtaa tttccccctg caacaatacc aaacggcaga
     5401 ttaacaatga gggcgattac aagccaccag aatagatctg aagcgctcag atcaaccaga
     5461 tatgggcgca aaagaaacag aatgaccagg gaaagggcaa ggagaatcag tcttaatact
     5521 gccgctgcag agtagaactc ttcctggtcc tttccctcgc tcatcctctt ggctgcggca
     5581 tttcccaaac ctccatcgac gatgagatta aaaagtccga aatatgccag aaacaggaaa
     5641 tatgctccaa ggatagaggg tccaagaaca tgggcaaaat agaaggttga gagaaatcca
     5701 accgcagtga tgacaatatt cgtgccgata tgaatcaggc tctggcgcct caccgcttct
     5761 atattcataa actgctgaaa aagcgcaggc ttctctgtca cacaactcca tttggtttca
     5821 aaggagatta tttcttgcga gtgaattatc ccggaaaacc tattagcctc caattccaag
     5881 aagagagcgt gagcatccct ttcgagcggg agtggaacct gatacctcat ttaatggtta
     5941 taagtaaccg aatcgctgta tcccagaaca ggacctccaa aaaaccttac aaggaggagt
     6001 ccggattcct tacaaaattt gacctcccaa agtattttct gctacgggtg tctggcagca
     6061 acatctattc tctgttctca cggattccta ggccgtgcaa gatatcccag cgatcaggac
     6121 ggccccaatg aagccgaaac tccgggtagt ccttgtaggg ccatcgatcc gcttcctttc
     6181 cgggatcagt tattatacca tcaggcttgc caatgcaata tcagaatcga cggatgtgac
     6241 ggcaatactc tttcgcaaca tgctgcccag gagaattttt ccggggtgga agcgggttgg
     6301 ggatcctctc tctacagtat cttacacggc tgaagtcaaa gtcatggaac tgcttgattg
     6361 gtacagtccc atttcgtgga tgagggctgc agcccagctg aggaaggcgg atatcatcat
     6421 tttccagtgg tggacgtcca gtgttgctca catgtatgtg gccctccagc ttatgcgctt
     6481 ccggaaagtt ccgattatta tcgaatatca tgaggtcgtt gatccactcg aacattcaat
     6541 tctcccaatc cggatatatt cggtcctcat ggggaggatc ataagaaagc gagccgatgg
     6601 ttatgtcgtt cattccgagg ctgacagggc tcttgttgaa acgtcttatc atgttcctca
     6661 agacaaagtg atggtgattc ctcacggcct ctatgaccaa taccctgtca tcgaacgaac
     6721 cattgcacgg aaacaactgg agatcgctga agaacacgtg atcctctttt ttggcctcct
     6781 tcggccctac aagggagtga aaaacctgat cgaagctttc gactcccttc ccactgaatt
     6841 gctgcactcc acccgcctcc tcattgtcgg ggaggcctgg gaggacaagg attctctggc
     6901 tcgtgctgag aaatcaccca acaggaacaa gatcacaatt gtcgaccgct atatcgggga
     6961 cgatgaggtc ccggtctact tttctgcctc cgacatcgtt gccctacctt acacccgtgc
     7021 ttcccagagc ggggttgcac atatcgcaat ggccttcggt ctctcaatta tcgcaacaaa
     7081 agtcggaggt ctcgaagagg ggttacaaac atattctggt gctctcttca tctctccaaa
     7141 caacaaggaa gagctgaaag atgcattgat acggcaaata gggaaaaaag attcatttcc
     7201 tcctccggca aacctcgcct ggagcgggat cggcaaacag tggactacat ttctatcgtc
     7261 ggtaatcctt aacagatcaa acgaaggtgc ctgaaatgaa gatcatccag gtctgccccc
     7321 gttattttcc cgatattgga ggagtagaaa ctcacgtcag ggagattgcc gaaagacttg
     7381 tcaaaaaggg gcacaaggtt gaagtggttt gcactgatcc atgcggagtc caccctaaaa
     7441 acgatttcat caatggagtt tcggttactc gattccggag ttttgcacca cactatgctt
     7501 atttttttgc gccgaagatg gccccctacc ttaaaacact agatgctgaa atcctccacg
     7561 ctcatgggta ccacgcattt ccagccttat tcgctatgat ggccgcacga aataagcgat
     7621 tcatcttcac tccccactat cacgggaaag gccatacccc tcttcgaaat atgcttctta
     7681 ccttttacaa tcccttaggg agggctatct ttaaccgtgc gaaccggatt atctctgtat
     7741 cagagtatga aaaaaatctg attataggca aattttgcat accggaggag aggtttagtg
     7801 ttattcccaa cgggctgaat ctgtcagagt tcaaggggat catcggggca aaagatccac
     7861 acaggatcct ccacgtaggc agaattgaac aatataaggg aatccagcat gtcattggag
     7921 cgctgccaga gttgccagaa ttcaccttca ccattattgg aaaaggtcca tatgaacatt
     7981 cgttgcaaca gttggctgcc gacaaaggag tgtcagatcg agtcacttgg aaaaaggatc
     8041 tttcgagaaa tgagctgcta caggaatatg catcggcaga attgttcatt tcgctctctt
     8101 catttgaagc ctatgggatt accgtagcag aagccttggc tagcggcctg caggtaattg
     8161 tcaataaaaa cggagcattg gaagagttcg ttgatggaac tacctgtatc gggattgaac
     8221 cttctgtaaa ggatgtaatc ggagccatca gattactgaa accatgccag ggctaccaga
     8281 aacgaatcat ggactggaat gaagttgttg agagaatcat agagacttac tcaagtggat
     8341 aaatagaatt attccgacta cattacactt catcgtattt acaaaaaaaa tattgaatgg
     8401 gaccacgctg atttaagatg gatttatgaa agttctccat tggaatatat caaaaaagaa
     8461 tcaaccgctg tcaggagtga aacggtacga agatgagtta ttcaccaata ttagaaaaat
     8521 tgatccttca ttggaaattg acaggatcca cagattggaa aatggctttt taggtagtac
     8581 tccgttatca tggctctatc gttatgctgt taaagatgcc gacattgtac atgcaacata
     8641 tcagacacta gcgcccagta tccggataaa aaaaccaaaa aagttcatcg ttaccgttca
     8701 tgatcttgct ccattagttt atcctgaact acaggataat ttctcaacaa aaattcaatg
     8761 gcagatgaca ccaagagggc tccgtaaagc ggattttatt attgcgatat caaattttac
     8821 aaaaaaggaa atcgtgagac tttgtaaaat tgacgagcag aaggtcgaag tagtacatca
     8881 gggggtcgat tctgagagat accacccatt ggataaagat aaaagtagaa aagagatcgg
     8941 attaaatcca gaaggtgagt atattcttgt tgttgcgtcc aatctcaccc ataaacggat
     9001 ggatttgacc aaaaaaatat tcgataacgt aaggaagcaa cgacccgatg tacagttaat
     9061 taaggttgga tatggggatg ttcttcaggg tcaaggaatt atcaacctag gttgggtaaa
     9121 agaggatgat atgcccttgc tttacaatgc ggctgatcta ttccttcaca cggctgaata
     9181 tgaaggattc ggccttccaa ttcttgaagc aatggcttgt ggttgttcgg taataggtat
     9241 gaatagagct tcaatacctg agattatcga aaattccggc attttaatga atacagatag
     9301 ggttaatgaa tggaccaggg ccattcttga tctttttgag aatgacgaat taagacggga
     9361 cctgcatcgg agaggcatga gaagaggaga gttattcagc tgggaaaaaa cagctcatga
     9421 aacccttagt atatataatt cttgacactt tttcctcgat attaactatt cttaccaatt
     9481 tgccatttaa cgaatttcca aaattttttt ataaatatcc acagtttttc gtacaacatt
     9541 ctctatcgaa ttatcagcgt tgtagttctc agcaaatttt gttagttgat ttcttaattc
     9601 gaaatctcta aaaagtctaa caatatcatc atggaggttt gtgtagatgt aagggtagtg
     9661 atactgacga tttgtatcat cgaagaatct gatcttagag gctaagcagg gaattttata
     9721 tgcagaaatt atattaaaaa ccccgctttg taaaattttt ttatagggca gaactgcaat
     9781 atcagcagca ctcattacca agggtatctt ttcatcggat acatatccga gaaattttac
     9841 cttgactgca ggatcatttg cgagaaaatg taagttctca agataccaac catctttccc
     9901 tttattgaaa ggatgtatat cccctgcaat aatcaatgtt gcatcgattc ccttcatggc
     9961 ttcgatgaga ttatccactc ctttatctcg atgaaggaat ccaaaaaata gaactgtctt
    10021 ttcatcagcc tcgatattta attccctttt tgcatttttt ttatccaatg gaaatgccgt
    10081 ataaagcacc ccgtgtggaa ttatttcaat tttatcctct ttgacacctg cctgtaatag
    10141 ccgttccttt gcctccgttg tatggacaat cacctttttt gaataaaatg atatgagcct
    10201 taaaataaga gcatccggta ttgatttatg taaaatctca tgaaacgttg ttattacata
    10261 tcgatcccgt gcgataattg gataaaatag ccaggcataa atcccatgga ttattttgat
    10321 accccagaaa aaaccatatt caaattgaac gtggattaac tttctggaac attcatctgc
    10381 cttttttatg taatagaatg gatttttatg gggttcgatg tagactggat gtatttcgca
    10441 atacttcttg agttcctcaa tgatatgatc agaatattca cctataccac aaacctttgg
    10501 gggagatgtc acaaaatcca atgaacattt atccatcttt tcctcaatga tcgcttctac
    10561 tcatatttga atgtggcata aaaaagccat cttttactcc ttttactaat ggaataacca
    10621 gctttggatc attaaagaat aatctcgaga ataaagttct ccaaaatttg taacccataa
    10681 aataaataaa aaaaattata aaaggcacaa caggcgcatt gtctttctca aaccaaattc
    10741 tatttcgggt gttgtaatat tcaaacgttg aatttgtacc tgattggctt cctttatgcc
    10801 atattaccgc ccgaggctca tacttgattt tatatccagc cctctttcct cggagacacc
    10861 aatctgtttc ttcccaatac aggaaatatg agggatttaa taaaccaatt ttttttatta
    10921 atgagctagt aaccagcaaa catgctcctg taatataatc aatgttacca acttcatcat
    10981 actgcccttt gtcaatctcc ttatccccaa tatggtgggt ttcacccctc cagatatcga
    11041 tttctcctcc acaatggcta aggatatcca ttcgattatt aagagtgtag taataaattt
    11101 ttggcccaat aaatccaata tttgaatcct gatccatgtc tgtgattagg gcatttaaaa
    11161 aagatttttc tacgactgta tcattattaa gtaataaaaa atattttgga tttagaaaat
    11221 tggctgaaaa ctgtatccca atattatttc cctctgcaaa tccgtagttt ttttcatttt
    11281 taatcagtat caacttacga tcagacgaga tattttcaat gtcatttttt ttccactcat
    11341 taaatttcaa ttcttctcgg gaaaattcaa atattttaat cggtttattt tgagttttat
    11401 actcaaaaaa aagtgattcc actttgaaat cgccattgca ataagccaaa attttttcaa
    11461 gggaatcatc taccgagcca ttgtctatca atactaccgt ataatttgga tattggatct
    11521 ggtagagaga ttctagacat tcgagggtgt ccctccagcc attccaattt agaataataa
    11581 ttgctacagt aggcttaatt aaaatacacc cctcgatttg ttttttcaat ttccccatat
    11641 actacaatga atttagcggg atctcatcct ttgaattaga atcagtccct aatttatatt
    11701 aaactccgtc aatttttttt tcaatgcatg aaaatataat atcatttaac tcattacaaa
    11761 tatcattcca attgtatctt ttctgcatat gactttttaa caaataggac atcccatcaa
    11821 agacatccgg gtgtgaaagg atattctgta aggcctttga aaagcactgt atatcatcta
    11881 cagggcaaag aacgccatat ctaccatcgt tcgttaattc tttaaatgaa ccaatattac
    11941 ttccaataag aatagttccc aaaattcccg attcagtgac aacaaggcca aaactctcaa
    12001 atcttgatgg ctgtaataaa atttttgctt ttgcatattc cctgaatata tcttttcggt
    12061 taggaagata tcctaaatag ttaatttggg ggccaatatt tgcatgagat tcgagaaaat
    12121 cggcgaaata ttttctaaat gaatcctcca taggaccaat taaaaccaac ttccagtgag
    12181 ggaatgaagg tgcgattgat gcaaaggact caagggctac atcagttcct tttgcgtatg
    12241 cccccaatct gccaatgtga acaatgatat cttctcgagc gggcgggcgt tctagcaatt
    12301 ctctgacaac cgggttttgg ctatcaaaac cgtttggtac gtaatgaagg caatcagcca
    12361 ctttcgtgaa caagggatgt tccttcttta agaaattgag agcaatttcc gtttctacac
    12421 taatgatatc aaaattagtc agttttaaaa taatatattg ataaaaaatc ctccatttca
    12481 tcttttttaa tttcctacgg agtgtttcta tcgaatcggg atccatatca agctttaaat
    12541 aaagaatccc tttaggatta tacaatctgt ataagagggc aaccagcctc gattcccatt
    12601 tgtaatgata taattggaat acagtaattt tattcctatt ttttaagaga aaacgccatg
    12661 caaaaagaat acgcagaaaa aatgattttg ggtaactatt gtcaggtgaa ttatcaatga
    12721 ttttattcga tgcacctttt ttttctcttg atttattgat aaaacatagt tttaaacctg
    12781 gagtttcaac atttaagtat gaatattcgt catttctata agtaagaatg taggaattat
    12841 acgagaatga tctatgtaga ataaaaggga tcatccctac atcttttgta aggtgaatat
    12901 ttttacattc aggaaaatag gtcaaaaaga caggattttg ggattcaagg atatcctgat
    12961 tcattatgtg atatttaaca ctgaactgaa aatgcttatt ataattacta tttgctagag
    13021 ccatatatcc gaacccttat ctctttcctg tcctattgat tccacatgac cccacctccc
    13081 tccggctgcc tgatctgtgg tgccgatctc gaataccttg accagccgga ggagatggta
    13141 tgccagtact gcaagcgggt ctgtaaatcc acaaccagat gcgtgaacgg ccactatata
    13201 tgcgacagat gccaccggct ggataccgag gacctgatcg aagtgatctg ccttcagagc
    13261 accgaagaca acccgttcac actcatagaa agaatcttct taagccctac cgtgatgatg
    13321 cacgggcccg agcaccattt cctggtgccc gcagtcctgg ttgctgcatt ttataatcat
    13381 acccagcagc ctgggaagaa agaactgggg atcaagaatg cacgacacag ggctgggctg
    13441 gtaaaggggg gatcctgcgg gctgctcggc gactgcggtg cagcggtcgg ggccgggata
    13501 ttcgtcagca tcgttaccgg agcaaccccg ctctcccgcg aggaatggcg catggccaac
    13561 ctgatgaccg cgagagcgct cgaaaagatc gcccttcatg ggggtccccg gtgctgtaaa
    13621 agaaacacgt tccttgcact gcaggaagcg atccggttca tctcaacaga gatggggatt
    13681 catctccctg aaccgaggga tatatcctgc cggttctctg accagaacag agaatgcctg
    13741 aaagatcgat gcccgtttta caatggaatc tgatctgatc agggttgata gagctaggca
    13801 attgcgagcc gaagtcgttg acagcgttcc ttcagctttt ttcttacaaa tttccttctg
    13861 ccattgaacg atgctcggaa aaaaggggtg cgatatcagt acagggatat ggggagaggg
    13921 aaatcccgtg cacctatctc ttccttattc ttccctgtac tgctccagaa ctttcctgat
    13981 aatccggctc gtgctcgagc acgagtcccc gggatactgg tcaatccgta caacagcgga
    14041 gcctattcct cgtgaccgga gatcagcgac cagctttgcc tcatcgaaat gctgattgaa
    14101 tccgatcgtg atgatcgcgg gctgaatctc gcggatcggc cggaacatgt catgcaggtc
    14161 accgagcacc gcgtgatcga ccggttttaa cgctccgacc atcttcatcc gctggtcttc
    14221 cggaataacc ggtcttggtt tgtgccggac attggcgtcc ctcgcaatga tcacgtacaa
    14281 ttcgtcaccg agcctgcgcg actcctccag gtaatagaga tgccccggat ggagcaggtc
    14341 aaaggttcct gtggcaacga ccgtcttcaa tcgatcaccc cgatatcaac ctgcatacca
    14401 ttagcggaaa aacagcgcca gtcatccacc tcatagggga atcccacgat gatgtgatat
    14461 ctcccggtcc tggggaagaa ggcgagatcg gcatcggatg ggcggatgac cccgttcgga
    14521 tgtgagtgga cgctcccggc gagatgcgta tcaagcggca tcatatcaag gaagaggctt
    14581 gcggaacgat cggtgctcgt tgtccccggc aggagattga tctcttcgat gataccctcc
    14641 tcttcacgga gaagcccggc atactcgttc gggtgctgct cccgcccaag ctggagcagg
    14701 agggagagca ggtcttttcg tattcccctg atacgcatat cctgccctag ttttaggacg
    14761 gaggagagat taaaccaggg ggtgcactcc cctggttcag aacggtccgt cctgctcaaa
    14821 acttctcccg aacctgatgg caagttccgc cttatagagc tctgcaccga gataggctgc
    14881 gtgatcgagg agcgaaacct ctccgcattc ctggatcgca tggaagacac ctgcccatga
    14941 acgcccccgg tatgcctttc cgccgataac cgcgacaatc tcctcgccct cgatcccgat
    15001 ccggaaattc ccgcagggat cataggtaat ctcctttggc atcgtttctg ccctcacctc
    15061 ctcgcaatac tccaggggag gttcccgcct cctccgcttc tccttgagta tgagcaggtc
    15121 gatcccgaga tccttcgggt agggccgttc cgcggcgagc accatcatct cggtcgctct
    15181 ccgcatctcc ctgaccgacc cccgggtctt gtccgagtgc tcgctggtga atatcaccga
    15241 cgctttcacc tccatggcca ttccggcaag gagggcgttc accccgggcg aatcggcatc
    15301 gatcagctct gccacgttcc ctgcgccgaa aaagagcgga tagcccggat cggtaaaatg
    15361 agagagggat gcgattagcc cggagccgac cggggcaagc agcgggtcag caatgatgca
    15421 ggagatcccg gcctcctctg caagcctgat attctcctca atggatcgct ctccaggaac
    15481 gaccaccgca cctgtaccac attctgccac cctccttcct acgagagcga tgttcctgtc
    15541 agagagcgag aggacaagat ccgcccgcgg aagccctgcc gcgatcaaca cgggatcctg
    15601 ggtgtcgatg gcgagagggc ctggaagatc cgaaagctcg gaaaagaccc gcttcacatc
    15661 accaggggtc gcatcaaacc cgaatccaag gtccacgata tcagcacccg atgaaaaaca
    15721 ggagagcgcc cttgcccgga tatccggttc tttgtgtgca tccatgatct cagccaggac
    15781 cttcatccgg gaatttcccc caatcttcac ccccctgatg agatactcag actccgcctc
    15841 ccgctccttc cgggcaatgg tccgtacagc ctcatcccgt cgctcgattg ccaggagatc
    15901 atcggcagcg atggtacggg acagggaaat cgagccaagg agggggagga tgagggcaag
    15961 atcagcggca tggcggggtc cccggtatat gggcaccccg gtccgctgtt caaccccgtg
    16021 gaaggatgcc gtgcacatgc cggagacaag gacaaggtcg tatttccctt ccccgatgag
    16081 ctcttccagc tgggcagggg tgagaaagga agcgatctcg cctgttaccg gtacaacagc
    16141 atccatcccc tccgctgcct ttctgaccat cccggcagtt gcagatccgg ttgggaggag
    16201 gatgcgcata agtttcctta ataccagtga gataaaaagt catatga
//
LOCUS       DAOOCO010000017        16131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3251_length_16131_cov_5.377519, whole genome shotgun sequence.
ACCESSION   DAOOCO010000017 DAOOCO010000000
VERSION     DAOOCO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 16131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16131
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3251_length_16131_cov_5.377519"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1666
                     /locus_tag="PK154_01925"
     CDS             377..1666
                     /locus_tag="PK154_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPW09852.1"
                     /translation="MFWNEKTETLKKRDLETIQLKRLKKTIAQVQNIGFYRNAFRQAG
                     IIPGSIRTIEDIRRIPFTRKQDLRDGYPFGFFAVPLSKVVRIHTTSGTTGKPTVVGYT
                     RKDLDTWAELIARNLTMVGLKEGDIFQNMVNYGMFTGGLGFHYGAEKIGLTVIPSATG
                     NTRRQIEMIQDFGVTAIHCTPSYAMHLAEVAEEMHADLPTLKTGLFGAEPWSDSMRKE
                     LEKKLGVTAYDSYGISELFGPGVAFECEERNGLHIWHDCYLVEIIDPATGEVVGDGER
                     GELVVTPLAKEAMPLLRYRTGDITMKLEDECLCGRSQKIGRITGRSDDMLVIRGINVF
                     PSQIEHVLLKIPEVGNQFMVYVDRINHLDEMTIDVEINRSFFSGELQDLARIQKKVVK
                     ELRDALELRTTVRLVEPGTLPRFEGKAKRVIDRRGAS"
     gene            1663..2964
                     /locus_tag="PK154_01930"
     CDS             1663..2964
                     /locus_tag="PK154_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPW09853.1"
                     /translation="MMFWDPRVEEMPREELQKLQYKLLKTLVYRLYSFSPFYHDRMKE
                     TGVHPDDIQTLADIRKLPFMYKRDLRDNYPDKIFVAGQDDLVRYHVSSGTTGKPTVVG
                     YTSNDLEHWTTSLARGLVSCGLGRGDVLQVSYGYGLFTGGLGLHYGAERVGATVLPTS
                     VGNTERQIELMQDLHTTAIACTPSYLLHMGEVAEKMGVSIRDDTDLRMGILGAEPWSD
                     SMKARIEDWLGIKAYDIYGTSELSGPMFCECSEQNGFHVWGDLAYVEILDPETGESLG
                     PGEKGELVVTMLQKEALPMIRYRIGDITSYDDGVCACGRTHPRVMRISGRVDDMLIIR
                     GINVFPSQIEYALMTIPEVGQHFQIVVDRKGALDDMLVRVEVSREAFSDKITDLMAIR
                     KKVEHKLRNTLNVAVNAELVEPGSLPRFEGKAKRVIDRRQL"
     gene            2964..3398
                     /locus_tag="PK154_01935"
     CDS             2964..3398
                     /locus_tag="PK154_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPW09854.1"
                     /translation="METDRYVIKQISIFSENRPGRLAAIAKALTEENINILAFSIAEA
                     DGFGVVRALVDKPKLAYEKLTGLGFNVAFTDVIAVKMRESPGGLYEIAKMLANVQINI
                     EYSYAYTGKEGGVLILRVDDADQAVRLITSLGATLLESRVFQ"
     gene            complement(3402..4688)
                     /locus_tag="PK154_01940"
     CDS             complement(3402..4688)
                     /locus_tag="PK154_01940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HPW09855.1"
                     /translation="MKRPVLQVALDLLDLSRALPIAREAVRGGADWIEAGTPLIKSEG
                     MESVRRLRSAFPGHVIVADMKVADTGALEVEMAAKAGASVVCILGDADDAVIAEAVRT
                     ASVFGVRLMADLINVSDPVVRSRELEALGVHILNAHVGIDQQMIGKSSLGLLEAIRGA
                     VAIEIAVAGGLDAHTAPQASARGADIVIVGGAITRSADVQDSARKVREALDAPASGYT
                     PKVSVPEETRDLLSGVSAPNISDAMHRKGAMAGIIYLAGKKKMVGTAVTAQSFAGDWS
                     KPVEAIDHAHEGDVLVINNEGSTDIAAWGELATMSAETQGIEGVVIDGAVRDIDDIRK
                     MSLPVFARAVVPNAGEPKGFGEINCEVRCGGQTVRPGDWIVGDESGVVVIPKERAYEV
                     ARRAREVQKTEERVREEIRRGSTLSRVSDLVKWEKK"
     gene            4863..5786
                     /locus_tag="PK154_01945"
     CDS             4863..5786
                     /locus_tag="PK154_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HPW09856.1"
                     /translation="MEILSIILTIAGLCLFETISSIDNAIINAEVLSTMAAWARRWFL
                     TWGLFFAVFVIRGLLPWLIVWMATPSLGPIEALTATFSNDPAVIAAIEESAPILLMGG
                     GIFLVFLFLHWIFLEEKHYGLRGECYIASKGVWFFAVVSILLATIVWFALAINPLMAF
                     AAVVGSTAFFIVHGFRQNAEEQERKMLGGTMSDWSKIFYLEVIDATFSIDGVLGAFAF
                     TLSVPLIIIGSGLGAIVVRQMTISNVDRIKRYLFLKNGAMYSILVLGSIMVLDAFGFH
                     IPTWLSPIATFGIVGFFYVKSIRNMPPAETA"
     gene            complement(6016..6360)
                     /locus_tag="PK154_01950"
     CDS             complement(6016..6360)
                     /locus_tag="PK154_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW09857.1"
                     /translation="MPDKDGCRELSPRECSDLLSRNLERPCRIIDVRTADEFRGGHLI
                     GAEHLDFYRPDFRQCIEQKDRTIRYLVYCRRGIRGQKTMELMHQCGFSDVVNIRGGYE
                     NWVSQGLPVQND"
     gene            complement(6459..6965)
                     /locus_tag="PK154_01955"
     CDS             complement(6459..6965)
                     /locus_tag="PK154_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW09858.1"
                     /translation="MNVGTTIIKCRHSVRSYKSVPVEDTIIRDAIECARFAPTAMNLQ
                     PWLIGVIRERDLLEKVATLTDHGRFIADAPVCFAVFGERKAKYYLEDCCAATENLIIA
                     LQSYGVGSCWVAGDKKEYAEPVRRLLGVPDQYTLVSLLPAGYPADIALQKKKEQDEIV
                     FFDQYKVD"
     gene            complement(7241..8203)
                     /locus_tag="PK154_01960"
     CDS             complement(7241..8203)
                     /locus_tag="PK154_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HPW09859.1"
                     /translation="MSISSWYALIILSALLALSPVLAAENTVPVAGTVFLGEQGLDIS
                     ATGAAAGSQIVYYGPGGSPATVPVATMTVDDPTDYYISPALFGGRTGPWFLSPANTLA
                     FYVQDPLIGIRVMDESADFAITSDVTWIPKGDVVGFRIDTNVYEIARRAGSPGAPITI
                     RIRGPGGVEYSSVTGYQLEDILISASPFDTGGVWLTGGFESGTYTVWAESTGNDMNLN
                     YKQEGKTVTVPVQFLLQSTNPLITPTPTTSPATTTTTPPPTLPATTVTTLTPATVITE
                     SPATTPGTVIPTTAPPTTTPGFGAMLTLSAFFVACLLFMARHQR"
     gene            complement(8249..9445)
                     /locus_tag="PK154_01965"
     CDS             complement(8249..9445)
                     /locus_tag="PK154_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW09860.1"
                     /translation="MAEIRIIGTAHVSKESVREVQEAIAEFSPDVVAVELDATRYAAL
                     KKQVQEASVESVLEAKTFTQLLVQWILAYLQRKIGLDVGVEPGAEMKAAVEEAEARRI
                     SIALIDRDIRITLRRFWHSLSFFEKIKMLYALVASVASIDEQEIDVEELKKQDVIAVA
                     MEEFRNFSPNGARALIDERDAYLAHQIRRLSLSHERILVVIGAGHKEGINRYLAHPET
                     LPSVESLTGEVKSRPWGLIFGGIVTFLFLFLLVAILFSGVGTDVLITALLYWVLIHGV
                     LTAVFTLLAKGHPLSALTGFAVSWFTALNPLIAAGWFSAIVEAKIRKPGPGDLGRISR
                     AESIGEMMEIPLFRVVVVAAMANIGSTLGTILYFVFIFPILGIDVGVVMTQGIANMWA
                     AIQAIF"
     gene            complement(9445..9978)
                     /locus_tag="PK154_01970"
     CDS             complement(9445..9978)
                     /locus_tag="PK154_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW09861.1"
                     /translation="MRQEELAAEIRRIGFSCTRCGECCRETDTDSNLVMLYPPESAEL
                     CTATGREGEEFSEPYPVAVVMPGGGSIRFERCIRRREGECIFLEGNRCQVYASRPWIC
                     RTYPFMLSGEDLMVFPCRGIGGTLSEGAAWEIAGRLLARNLAEKEEEERVRTLLSSNR
                     IPAGAEVLIDGTGMRIL"
     gene            complement(9986..10543)
                     /locus_tag="PK154_01975"
     CDS             complement(9986..10543)
                     /locus_tag="PK154_01975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HPW09862.1"
                     /translation="MLPIYVVNNFGQFNHLILRMLRDRSVDARMISNSMPPEDVAAGC
                     RGLILGGGPDLSRSGNSARYLHLDLPVLGICLGLHVIAREFGGTVTKGRLGGYGPVEV
                     EIVEHDEILIGYPSRIRVWASHADEVTGLPAGAVRLAGSPICSNEAIALPDRHIYGLQ
                     WHPEVSHTENGRYVYENFDRITREQ"
     gene            complement(10660..11670)
                     /locus_tag="PK154_01980"
     CDS             complement(10660..11670)
                     /locus_tag="PK154_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HPW09863.1"
                     /translation="MAFVTGSSEIRAKNPLFSSILANTMLSVVPGISGAGPSPEKTLL
                     TPILDAELITCGKITSMPVRPDTPTGCPTPASITRAVMELCGLSPLFINGGLAHTPTV
                     PCLDTYGIPGEDPRFRDAVPDAGALFRKGRSIGTFLGSVSDLLVLGECVPGGTTTSLC
                     VLRGLGYDALVSSSYARNPVRMKEEVWSIVNRRIQEGLYRTPLDIVRCCGDPMIAIAA
                     GIAATYSGSIILGGGTQMLSVAAVLKGMNLPAPGIATTCYVRDDASANFAEMAGMIGT
                     PVYYVDPGFGELGHSGLARYCIGEVKEGMGAGGAMFLAWLMGHSPDAIRKKILHTVHG
                     YA"
     gene            11759..12193
                     /locus_tag="PK154_01985"
     CDS             11759..12193
                     /locus_tag="PK154_01985"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPW09864.1"
                     /translation="MFGIEERLKEKGISVDSMVTSAMLLYVPHGLSAEEAAHKVREKI
                     LKYLGDPNVASLLLGAILLEDELFLRQKDSGIQEDPVFLLSDEIIGMAIAECIGGTYA
                     RFEFTRYDQKKPGILATLGPFLDDAVAGLIAGCTSRLYSECC"
     gene            12184..12918
                     /gene="cobS"
                     /locus_tag="PK154_01990"
     CDS             12184..12918
                     /gene="cobS"
                     /locus_tag="PK154_01990"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPW09865.1"
                     /translation="MLLNRLRALLQFTTILPLGRTVDFSLFASHTYLYPLSGYVIGGL
                     AGLTAFWISIPEIRAAVALIAVLGITGANHFDGLLDLGDGLMAHGGREGRIRALTDRQ
                     IGTGGFAAGCTVLLLTFAALSSLPEILWALVAAEVLAKFSMALLTAYGAPFHDGLHAY
                     LYHRAKPWFPVAAGICCLPLVLLPLPYLPLSGAVLAALLIPFVLLVIADRLFGGVNGD
                     VVGASGEITRAVVLCALVLIPASALF"
     gene            12945..13856
                     /locus_tag="PK154_01995"
     CDS             12945..13856
                     /locus_tag="PK154_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW09866.1"
                     /translation="MLKDMGVHMKGGVITELDSEYCTIRTRIPAGVVSCDQLKGIATV
                     AERYGAESVHLTTRQTIEIPHMNHADLDAIARDLALNNTPIGSERDEVVNVIACPGNE
                     RCIFANIDSISLARSIDARLFGREMPVKIRISISACPNACTSPLLNEIGIVGQNRPIR
                     VPGMCTGCGTCVEYCKEEAITIKRGVSVLDPSRCVECGTCIRSCPYDLLKSSGEHYLI
                     TVGGRRGRHPKIGRELIAVKSPESVLLVVEKVVAWVYRRAWSGRLLSDQMDELQFETF
                     RDEIRALVPEKDRAEEIIGDFSGGSGI"
     gene            complement(13834..14136)
                     /locus_tag="PK154_02000"
     CDS             complement(13834..14136)
                     /locus_tag="PK154_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09867.1"
                     /translation="MKTLEITWDEKKYRATVGCADGSVSVHRNCAFCVHCKGIRIGER
                     LYPSPQESVLRSFSSGGSSDEALMSAAMQFNTLVKDARAIECDDDADSGFKSRYRR"
     gene            14311..15588
                     /gene="tuf"
                     /locus_tag="PK154_02005"
     CDS             14311..15588
                     /gene="tuf"
                     /locus_tag="PK154_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HPW09868.1"
                     /translation="MAVEKPHLNLAVIGHIDHGKSTTVGRLMFETGAVPPHIIEGYRK
                     EAETKGKATFEFAWVMDNLKEERERGITIDIAHKRFDTPKFYFTVVDCPGHRDFVKNM
                     ITGASQADAAVLVVAAPDGVMEQTKEHVFLSRTLGINQLIVAVNKMDAAKYDQKRFEE
                     VKKDLSALLQMVGYKPAETLFIPISSLQGVNIAKKSPETPWYTGPTLLESLDTLKEPE
                     KPTDKPLRLPIQDVYSISGIGTVPVGRVETGIMKKGMKVSFMPANKEGEIKSIEMHHE
                     EIPQALPGDNVGFNVRGLAKGDIRRGDVCGPVDAPPTVADEFTAQIVVLQHPSAITIG
                     YTPVFHCHTTQTACTFVELKKKLDPRTGQTKEENPTFIKAGDAAIVQVKPTKPMVIEN
                     VKELPQLGRFAIRDMGSTIAAGMCIAIQPKQMR"
     gene            15623..15931
                     /gene="rpsJ"
                     /locus_tag="PK154_02010"
     CDS             15623..15931
                     /gene="rpsJ"
                     /locus_tag="PK154_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPW09869.1"
                     /translation="MQKARIRLTGTDFKKVEMVCDRIREIAERTGVNIAGPIPLPTKR
                     LVVPIRKSPDGEGTATWDRWQMRVHKRLIDIDADERALRQLMRTQVPKDIGIEIVLES
                     "
     gene            15941..16111
                     /locus_tag="PK154_02015"
                     /pseudo
     CDS             15941..16111
                     /locus_tag="PK154_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
BASE COUNT         3554 a         4753 c         4457 g         3367 t
ORIGIN      
        1 caccaaaccc agtcacaata tgccatggat accgcacgaa gaactccttt ccaaaaaacg
       61 acctggaccc tttgctcgca ggaatgaata tgggcagccc cctacatcaa ttcatcgttt
      121 tccagttcaa tccccgatac tccggggaaa acacctcttt ttctggagaa ggaggattcc
      181 ctgtttccag caccattttt cctctcaatc cgggaggata tcaaaagcag gcacatcgga
      241 ataatcccac catgacagcc cctgacatag ttttttgacc gaatgcgacc gatatatgca
      301 tgtttgacag gtcagccggg ccgggtccgc caaaaaccaa ccggctgccc ggcatacccg
      361 atgaaaggag gatgtgatgt tctggaatga gaagaccgag accctgaaga agagagacct
      421 tgagacaatc cagttgaagc ggctgaaaaa gaccatcgca caggtccaga atatcggctt
      481 ttacaggaat gccttcaggc aggcggggat cattccgggg agtatcagga caattgagga
      541 tatccgccgt atccccttca ccaggaaaca ggatctccgt gacgggtatc ctttcggatt
      601 ctttgcagtc ccgctctcga aggtggtccg tattcacacc acctccggca cgaccgggaa
      661 gcccaccgtg gtgggatata cccggaagga tctggacacc tgggcagagc tgatcgcccg
      721 gaacctcacc atggtgggat taaaagaggg ggacatcttc cagaacatgg tcaattacgg
      781 gatgtttacc ggagggcttg gatttcacta cggtgcagag aagatcgggc ttaccgtgat
      841 tcccagtgcg acgggcaata ccaggcgcca gatcgagatg atccaggact tcggggtgac
      901 ggcaatccat tgcacgccaa gctatgcgat gcacctcgcc gaggtcgccg aggagatgca
      961 cgccgatctc ccgacactga agaccggcct tttcggggca gaaccctggt ccgattccat
     1021 gcgaaaggag ctggagaaga aactgggggt caccgcttat gactcctacg gtatctcgga
     1081 actcttcggc ccgggagttg cctttgaatg cgaggagcgg aacggactgc atatctggca
     1141 cgattgttac ctcgtcgaga tcatcgatcc ggctaccgga gaagtggtgg gagacggcga
     1201 gcgcggagaa cttgtcgtaa ccccccttgc aaaggaggcg atgccgctcc tgcggtaccg
     1261 gaccggtgac atcaccatga aactcgagga tgaatgtctc tgcgggagat cccagaagat
     1321 cgggcggatc accggacgga gtgacgatat gctggtgatc aggggtatca acgtcttccc
     1381 ctcccagata gagcatgtcc tcctgaaaat acccgaggtg gggaatcagt ttatggtcta
     1441 tgtcgaccga atcaatcacc ttgatgaaat gaccatcgat gtcgagatca accgatcctt
     1501 cttctccggg gaactccagg accttgcccg gatccagaag aaagtggtaa aagagctacg
     1561 cgatgccctt gaattgcgca ccaccgtcag gctggtcgag ccgggaaccc tcccgcggtt
     1621 cgaggggaaa gcaaaacggg tgatcgacag gagaggagca tcatgatgtt ctgggatccg
     1681 agagtcgaag agatgccaag ggaggagctg cagaagctcc agtacaaact tttgaaaacg
     1741 ctggtatacc gtctctacag cttctccccc ttctaccatg acaggatgaa ggagacaggc
     1801 gtccatcccg acgacataca gaccctggcc gatatcagga agctcccgtt catgtacaaa
     1861 cgggacttgc gtgacaacta ccccgacaag atctttgttg ccggccagga tgacctggtc
     1921 cggtaccatg tctcttcggg gacgaccggg aagcccaccg tagtgggata taccagcaat
     1981 gacctcgaac actggaccac ctcccttgcc cgaggtcttg tctcctgcgg gctcgggcgg
     2041 ggtgatgtcc tccaggtcag ctacgggtac ggcctgttca ccggcggcct cgggctgcac
     2101 tacggggcag agcgggtcgg tgcaaccgtg cttccgacca gtgtcgggaa cacggaacgc
     2161 cagatcgagc tgatgcagga tcttcatacg accgccattg cctgcacccc ctcctatctc
     2221 cttcacatgg gggaggtcgc agagaagatg ggcgtgagca tcagggatga taccgatctc
     2281 cgcatgggaa tactcggtgc agaaccctgg tctgattcca tgaaagcccg gatcgaggac
     2341 tggctcggaa taaaagccta tgacatctac ggcaccagcg agctctccgg gccgatgttc
     2401 tgcgaatgtt cggagcagaa cggctttcat gtctggggag acctcgccta tgtcgagatc
     2461 cttgatccgg agacgggaga gtcactggga ccgggggaga aaggcgaact ggtggtcacg
     2521 atgctccaga aagaagccct tccgatgatc aggtaccgga tcggggacat cacctcgtac
     2581 gatgacgggg tctgcgcctg cggacggacg catccccggg tgatgcgcat ctccggcaga
     2641 gtcgatgaca tgctgatcat caggggtatc aatgtcttcc cctcgcaaat cgagtatgcc
     2701 ctgatgacca tcccggaggt cggccagcat ttccagatcg tcgtggaccg gaaaggggcc
     2761 cttgatgaca tgctggtcag ggtcgaggtg agcagggaag cgttctctga caagatcact
     2821 gacctgatgg caatcaggaa aaaagtcgag cacaagctca gaaacaccct gaatgttgcc
     2881 gtgaacgcgg aactggtcga accgggatca ctcccccggt ttgaggggaa ggcaaaacgg
     2941 gtgatcgaca ggaggcagtt ataatggaaa cagaccggta tgtgatcaaa cagatctcga
     3001 tattttccga gaacaggccc ggccggcttg ccgcaattgc aaaagccctc accgaggaga
     3061 atatcaacat ccttgcattc tccatcgcag aggcggacgg gttcggtgtt gtcagggcgc
     3121 ttgtcgacaa gccaaaactg gcctatgaaa agttgacagg cctcggattc aatgtcgcat
     3181 tcaccgacgt gattgcggta aagatgcggg aatcccccgg aggactgtac gagattgcaa
     3241 agatgctggc gaacgtacag atcaacatcg aatactcgta tgcctacacc gggaaagagg
     3301 gaggagtcct gatcctccgg gtcgatgatg ctgatcaggc agtccggctc atcacatcgc
     3361 ttggtgcaac actcctcgag agcagggtct tccagtaatc cctacttctt ctcccatttc
     3421 accaggtcgg agacccggga gagcgtactc cccctcctga tctcctccct gacccgctct
     3481 tcggtcttct ggacctccct cgcacgccgg gctacctcat acgcccgctc ctttggaatg
     3541 accacaaccc cgctctcatc accgacaatc cagtcacccg gccggacagt ctgcccgccg
     3601 cacctgacct cacagttgat ctcgccaaac ccttttggct ccccggcatt cgggaccact
     3661 gcccgtgcaa ataccggcag tgacatcttc ctgatatcat cgatatccct gacagcccca
     3721 tcgatgacca cgccttcgat cccctgggtc tctgcactca tggtcgccag ctctccccat
     3781 gccgcgatat cggtactccc ctcgttgttg atcacgagga catctccttc gtgtgcatgg
     3841 tcgatagcct cgaccggttt tgaccagtca ccggcaaacg actgagcggt gactgcggtt
     3901 ccgaccatct tttttttccc ggcaagatag atgatcccgg ccatcgcgcc tttccggtgc
     3961 atcgcatccg agatattcgg ggctgaaacc ccagagagga ggtcccgggt ctcctctggc
     4021 accgagacct tgggggtata tccgctggca ggggcatcca gcgcttcccg gactttccgt
     4081 gccgagtcct gcacatccgc agaacgggtg atcgcacccc cgacaatcac gatatcagca
     4141 ccccgtgccg atgcctgggg ggcagtgtgt gcatcgaggc cgcccgcaac cgcaatctct
     4201 atcgctaccg ctccacggat cgcttcaaga agccccagcg agctcttccc gatcatctgc
     4261 tgatcgatcc ccacgtgggc attgaggata tgcactccga gagcctcaag ttcacgggag
     4321 cgtaccaccg ggtcactcac attgatcagg tcggccatca gcctgactcc gaagactgag
     4381 gcggtcctga ccgcttctgc gatcaccgca tcatcggcat caccaaggat acagaccacc
     4441 gaagccccgg cttttgctgc catctccacc tcaagcgccc cggtatcggc tactttcatg
     4501 tcagctacaa tcacatggcc gggaaatgca gaccgcaatc gcctgaccga ctccatccct
     4561 tcactcttga tgagcggggt ccccgcctcg atccagtcag caccacccct gactgcctcc
     4621 cgggctatcg gcagagccct cgagaggtca agcagatcca aggctacctg cagtacaggc
     4681 ctcttcatga tcactgaggt gaggagagag ggcataatag tttgccgggt acccgtctgt
     4741 gtcccccggt ctttgccgcg cttccatggt atcatactaa aaaaggtccg gattccagcc
     4801 atttccctgc tccatccggg aatcttttat gggaatcatc accaacactc tgacgtacag
     4861 caatggaaat cctgtcgatc atccttacca tcgcagggct ctgcctcttt gagactatca
     4921 gcagcatcga caatgccatc atcaatgctg aggtcctctc caccatggcc gcatgggcgc
     4981 ggcgatggtt tctgacgtgg gggctttttt ttgcggtctt cgtcatccgg gggctcctcc
     5041 cctggctgat cgtctggatg gccaccccct cgctcgggcc aatcgaggca ctgacggcaa
     5101 cgttttcaaa cgacccggcc gtcattgccg ctattgaaga gtcggccccc atactcctga
     5161 tggggggtgg catcttcctg gtgtttctct tcctccactg gatattcctg gaagagaagc
     5221 actacggcct ccggggggag tgctatatcg cctcgaaagg agtctggttt tttgccgtcg
     5281 tctcgatttt gctcgccacc atcgtctggt ttgcgctcgc gatcaatccg ctcatggcat
     5341 ttgcagccgt cgtgggctcc accgcatttt tcatcgtgca cgggttccgg cagaatgccg
     5401 aggagcagga acggaagatg ctcggcggca cgatgtcgga ctggagcaag atcttttacc
     5461 tggaggtgat cgatgccacc ttctctattg atggcgttct tggtgccttc gccttcacgc
     5521 tctccgtgcc cctcatcatc atcgggagcg gccttggcgc tattgttgtc cggcagatga
     5581 ccataagcaa tgtcgacaga atcaaacggt atctcttcct caagaacggg gccatgtact
     5641 cgatcctcgt gctggggagt atcatggtcc tcgatgcgtt cgggttccat atcccgacat
     5701 ggctctctcc cattgccacc ttcggcatcg tgggattttt ctatgtaaaa tcgatcagga
     5761 acatgccgcc tgctgaaacg gcatgaaggc cttattttcg caatcccctc tctttttcgc
     5821 tctgttccgg ggtgacccgg cgttccattc ccggcatgcg gagggagaag gacgtattcc
     5881 ccagagccgg cacccgggag catacacccc gaccggccaa ctgaagaagg gttggtttca
     5941 ggtcgggatg gtggttgctc caggctgaag ggaaacaatg gaattcacct ccggcaccgg
     6001 tttcactggt cgatatcagt cgttctgtac cgggagcccc tgtgacaccc agttctcata
     6061 gccaccccga atattcacca catcggaaaa gccacactgg tgcatcagct ccatcgtctt
     6121 ttggcccctt atcccccgtc tgcagtagac caggtacctg atcgtccgat ccttctgctc
     6181 aatgcactgc ctgaaatcag ggcgatagaa gtcaaggtgc tcggccccga tcaaatgccc
     6241 gccgcggaac tcgtcagcag ttctgacatc gatgatgcga cagggacgct ccaaattcct
     6301 ggagagaagg tcagaacatt cccgcgggga gagttcccga cacccatcct tgtctggcat
     6361 agttcttctc tagcatcctg gaaaagcccg gaaacgggag accaccttct catacgccct
     6421 gtggctggag acggaaagcg tctgaatccc ccgcatggtc agtctacttt gtactgatca
     6481 aagaagacga tctcatcctg ttccttcttc ttctggagcg cgatgtctgc cgggtaacca
     6541 gcgggcagga gagagacaag ggtgtattga tcaggaaccc ccagcagtct ccggaccggc
     6601 tctgcgtact ccttcttgtc accggcaacc cagcaggacc ccaccccata ggactggagg
     6661 gcgatgatca ggttctcggt ggctgcacag cagtcctcga ggtaatactt cgccttccgt
     6721 tcaccaaata cggcaaaaca gaccggggca tcggcaatga acctcccgtg atcggtcagg
     6781 gtggcgacct tctccagcag gtctctttcc cggatgaccc cgatcagcca gggctggaga
     6841 ttcattgcag tcggcgcgaa gcgtgcacat tcgatggcat ccctgatgat cgtgtcctcg
     6901 accggaacgg atttgtaact ccggacactg tgccggcatt tgatgatggt ggttccgaca
     6961 ttcatgagta tccccttgca gtcaagggaa aaaatggttt tggcctgctt attcccgcca
     7021 gggacggatc gatgagctga agtctgtcac tatgctctac agggacaaag atcatgtata
     7081 gttcaataga tggcactctt atggtcggac taagtaaaac cacaggaaag atcatgttcg
     7141 cggaacgagg gaattgaagg gagagggatc gccgtcactc cgcgctgctg gatggtcgga
     7201 agttccctga ccttctccct gaaaaggggg aggggtgggc ctacctctgg tgcctggcca
     7261 tgaacaggag gcaggcgaca aaaaaggcgg agagggtgag catggcacca aagcccgggg
     7321 tggtcgtggg gggggccgtg gtaggaataa ccgtaccggg cgttgttgca ggagattccg
     7381 taataacagt ggcaggggtg agcgtagtca ccgtcgtagc aggaagggta gggggagggg
     7441 ttgtggtggt cgtggccggg cttgtggtgg gtgttggggt gatgagcgga ttggtgctct
     7501 ggagaaggaa ctgcactggc acggtcaccg tctttccctc ctgcttgtaa ttgagattca
     7561 tatcattccc ggtgctctcc gcccataccg tgtaggtgcc actctcaaaa ccgcccgtca
     7621 gccatacccc tcccgtatcg aatggcgagg cagatatcag gatatcctca agctgatacc
     7681 ctgtcaccga agaatactca acaccccctg gaccccggat gcggattgtg atcggagcac
     7741 ccggggaacc tgcccgtctg gcaatctcgt agacattggt gtcgatccgg aatcccacca
     7801 cgtccccctt cgggatccag gtgacatcgc tcgtaatcgc gaaatcggcg ctctcatcca
     7861 tcacccggat cccgatcagc gggtcctgga catagaacgc aagggtgttt gccggggaca
     7921 gaaaccaggg gccggtcctg cctccaaaga gagccggaga gatgtaatag tcggtgggat
     7981 cgtccaccgt catggttgcc acgggaacag tggcagggct cccgcccggc ccgtaataca
     8041 cgatctgcga accggctgcg gcaccggtcg cggagatatc aaggccctgc tctccgagga
     8101 agaccgttcc ggcaaccggt acggtatttt ctgctgcgag gacaggggag agcgcaagga
     8161 gtgcagaaag gatgatcagg gcataccagg acgaaatgct catatgccac tcatcgcggg
     8221 gacactaaaa atagtttctg ggaggcgatc agaagatcgc ctggatggcg gcccacatgt
     8281 tcgcaatccc ctgggtcatc accaccccga catcgatccc gagtatcggg aagatgaaga
     8341 caaagtagag gatcgtcccg agcgtgctcc cgatatttgc catggcggca acaacgacca
     8401 cccggaagag cgggatctcc atcatctctc cgatcgactc agcccgcgag atacggccca
     8461 gatcccccgg tccgggtttc cggatctttg cctcgacgat cgcggagaac cagccggctg
     8521 cgatcagcgg gtttaatgcc gtgaaccagg agaccgcaaa tccggtcagg gctgaaagag
     8581 gatgcccttt tgcaaggagg gtgaacacgg ccgtaaggac cccgtggatc agcacccagt
     8641 aaaggagggc agtgatcagg acatctgttc ccacgcccga gaagaggata gccaccagga
     8701 gaaagaggaa gaggaaggtg acgattcccc cgaagatcaa cccccacggc ctggacttca
     8761 cctcacccgt aaggctttcg acagagggca gcgtttcagg gtgtgcgaga taccggttta
     8821 tcccctcctt gtggcccgca ccgatcacca cgagaatgcg ttcatgagag agagagagcc
     8881 tccggatctg gtgggcaaga tacgcatcgc gttcatcgat cagggcccgg gcgccattcg
     8941 gtgagaaatt ccggaactct tccatcgcga cggcaatcac gtcctgtttc ttcagctcct
     9001 ccacatcgat ctcctgctca tcgatactgg caacggatgc aacgagggca tagagcatct
     9061 tgatcttctc aaaaaacgag agtgaatgcc agaacctccg gagagttatc cggatatccc
     9121 ggtcgatcag ggcgatgctg atgcgcctgg cttcggcctc ttccacggct gctttcatct
     9181 ctgcccccgg ctctacgcca acatcaagcc cgatcttccg ctgcaggtaa gcaaggatcc
     9241 actggacaag gagctgggtg aacgtctttg cctcaagcac cgattcgacc gaagcttcct
     9301 ggacctgttt tttcagggct gcataccggg tggcatccag ctccaccgcg accacatcag
     9361 gagagaactc agcgattgcc tcctgcacct cgcggacact ctctttcgag acatgggcag
     9421 ttccgatgat ccgaatctca gccatcagag tatcctcatc ccggttccat cgatcagcac
     9481 ttcagcacct gcagggatgc gattcgagga gagcagggtt cgaacccgct cctcctcctc
     9541 cttttcggcc agattccgtg cgagaagacg cccggcgatt tcccaggctg caccttctga
     9601 gagggtcccc ccgattcccc tgcacggaaa gaccataaga tcctctccgg aaagcatgaa
     9661 cgggtaggtc ctgcagatcc atggccgtga cgcatatacc tggcatctgt ttccctcaag
     9721 gaagatgcat tccccttccc tccgccggat gcatcgttcg aacctgatac tcccccctcc
     9781 cggcatcacc accgccacag ggtacggttc cgagaattcc tctccctccc ggccagtggc
     9841 agtgcagagt tccgcactct ccggaggata cagcatcacc agattggaat ccgtatcggt
     9901 ttccctacaa cattctccgc accgggtgca ggagaacccg atcctcctga tctctgctgc
     9961 cagttcttct tgtctcatct gcttttcact gttccctggt aatccggtca aaattctcgt
    10021 agacatacct gccgttctcg gtatgactga cttcaggatg ccattgaagg ccgtagatat
    10081 ggcgatccgg taatgcaatc gcttcattgc tacagatcgg tgagccggca agcctgactg
    10141 ctcctgcggg aaggccggtg acctcatcgg catgggaggc ccatacccgt atccttgacg
    10201 ggtatccgat caggatttcg tcatgctcga caatctccac ctcgacagga ccgtacccgc
    10261 ccagcctgcc cttggtgacc gtacccccga attcccgtgc gatgacatgg agccccagac
    10321 atattccgag caccgggaga tcaagatgga ggtatcgtgc agagttcccc gagcgggaga
    10381 ggtcaggtcc tcccccgagt atcaggccac ggcaccctgc ggcaacatcc tcagggggca
    10441 tgctgtttga gatcatcctt gcatcgacag aacgatccct gagcatccgc aggatcaggt
    10501 ggttgaattg cccgaaatta ttgaccacat atatcgggag catccctgat cacttctctc
    10561 tgatggctga taatcccttt tttatcggag ccttccggaa atcccctccc ggccggcctg
    10621 gctattcccg tcaccaaacg atgtaccaca cccctgttcc tacgcatacc cgtggaccgt
    10681 gtgaaggatc tttttcctga tcgcgtcagg agaatggccc atcagccagg caaggaacat
    10741 ggcgcctccg gcacccatcc cttccttcac ctccccgatg cagtaccgtg caagccccga
    10801 atgtcccagt tcaccgaagc cgggatcgac gtaatacacg ggtgtcccta tcatccctgc
    10861 catttcagca aagttggccg aggcgtcatc cctcacataa caggtggttg cgatcccggg
    10921 tgccgggaga ttcatccctt tgaggaccgc tgcaacagag agcatctggg tcccccctcc
    10981 gaggatgatg ctcccgctat acgtggctgc gatcccggcg gcaatggcga tcatcggatc
    11041 cccgcagcaa cggacgatat caaggggggt gcggtagagg ccttcctgta ttcttctgtt
    11101 cacgatgctc cagacctcct ccttcatccg gacaggattc ctggcatagc tgctgctcac
    11161 cagggcatca tacccgagcc cccgcagaac acagagcgac gtggtggtcc ctccgggaac
    11221 acattccccg agaaccagaa gatccgaaac actcccaagg aaggtcccga tcgacctccc
    11281 tttcctgaac aatgcaccag catcagggac cgcatccctg aaccgggggt cttcgcccgg
    11341 tatgccatag gtgtcgaggc atggtacggt cggggtatgg gcaagccctc cgttgatgaa
    11401 aaggggcgag agtccgcaga gttccataac cgccctcgtg atggatgcag gcgtggggca
    11461 gcccgtcggg gtatcggggc gcaccggcat gctggtgatc ttcccgcagg tgatcagctc
    11521 cgcgtcaagg atgggggtga ggagcgtctt ttcagggctt ggaccggcac ccgagatgcc
    11581 tggcaccaca gagagcatcg tattggcaag aatactggaa aaaaggggat tctttgccct
    11641 gatctccgag ctgcctgtca caaatgccat gagtactcga tcgtacagct ctcaccttcc
    11701 cggacatcaa gctatccttg cggcaaccta atagcggatg caggaacaca tagcagatat
    11761 gttcgggatt gaggaacggc tgaaggagaa agggatctct gtcgattcca tggtaacctc
    11821 tgcgatgctg ctctatgtgc cgcacgggct ttcggcagaa gaggcagcgc acaaggtacg
    11881 ggagaagatc ctcaagtatc ttggcgaccc gaatgtcgca tcccttctcc tcggggctat
    11941 cctccttgaa gatgaactct ttctccggca gaaagattcg ggaatccagg aagacccggt
    12001 attcctcctc tccgatgaga tcatcgggat ggccatcgcc gaatgtatcg gcgggaccta
    12061 tgcccgtttc gagttcacgc ggtatgacca gaagaaaccc ggcatcctgg caacactcgg
    12121 cccgttcctc gatgacgcgg tcgccgggct gatagccggt tgtacatccc gcctttacag
    12181 cgaatgctgc tgaaccggct ccgggccctc ctccagttca cgaccatcct ccctctcggc
    12241 aggacggttg atttttccct ctttgcaagc catacctacc tctatcccct ctccgggtac
    12301 gtgatcgggg gtcttgccgg gctcactgcg ttctggatca gcatcccgga gatcagggca
    12361 gcggtggcac tcattgccgt gctgggtatc accggagcaa accattttga cgggctcctc
    12421 gatctgggcg acgggctcat ggcacatgga ggaagggagg gcaggatccg ggcactcacc
    12481 gaccggcaga tcggcaccgg cggatttgct gccgggtgca cggtcctgct cctaaccttc
    12541 gccgccctgt catctctgcc ggagatcctg tgggccctgg ttgcagcgga agtccttgca
    12601 aaattctcca tggcattgct gacggcatac ggggccccgt ttcatgacgg cctgcatgca
    12661 tacctgtatc accgggcaaa gccctggttt ccggtcgccg ctgggatatg ctgcctcccg
    12721 ctcgtgctcc tcccgctccc gtatctcccc ctttccggag cagtccttgc ggcgctcctc
    12781 atcccgttcg tccttctcgt cattgcagac cggctgttcg gaggcgtcaa tggcgatgtc
    12841 gtgggagctt ccggggagat aacccgtgca gtggtcctct gcgcactggt cctcattcct
    12901 gcgagcgcac ttttttaggg gatgcaccgc gtaataagtt cctgatgctg aaggatatgg
    12961 gagttcacat gaagggcggt gtcatcaccg agctcgacag cgaatactgc acgatacgaa
    13021 cgcgaatccc ggcaggcgtc gtctcctgtg accagctgaa agggatcgcc acagtggcag
    13081 agcggtacgg ggctgaatcg gtccatctga ccacccggca gaccatcgag ataccgcaca
    13141 tgaaccatgc agacctcgat gcgattgcac gggatctcgc actcaataat acccctatcg
    13201 gctccgagcg tgatgaagtg gtgaacgtga tcgcatgccc ggggaacgaa cggtgcatct
    13261 ttgccaacat cgactcgata tcgctcgccc gctccatcga tgcaagactt tttggaaggg
    13321 agatgccggt aaagatacgg atctccatat ctgcctgccc gaatgcctgc acgagcccgc
    13381 tcttaaatga gataggcatc gtcgggcaga accgtcccat cagggtgccc gggatgtgca
    13441 ccggatgcgg gacatgcgtg gaatactgca aagaagaggc gatcacaata aagcggggag
    13501 tttccgttct cgatccctcc cggtgtgttg aatgcggcac ctgtatcagg tcctgcccgt
    13561 acgatctcct caaatcatcc ggcgagcatt acctgatcac ggtcggaggc cggaggggga
    13621 gacaccccaa gataggcagg gagttgatcg cggtaaaaag tcctgaatcg gtactcctcg
    13681 tggtcgagaa ggtggtggcc tgggtatacc ggagggcatg gagcggcagg ctcctttcag
    13741 accagatgga tgagttgcag ttcgaaacat tccgggatga gatacgggca ctggtccccg
    13801 agaaagaccg cgcagaagaa atcattgggg atttcagcgg cggtagcggg atttgaaccc
    13861 cgaatccgca tcatcatcgc attcgatggc ccgtgcatcc tttaccagcg tgttgaactg
    13921 catcgcagct gacatgaggg cttcatcgct cgaaccaccc gatgagaagg atcgcagtac
    13981 cgactcctgg ggtgacgggt acagccgctc ccctatccgg atccccttgc aatgcacaca
    14041 gaacgcgcaa ttccggtgaa cggataccga cccgtctgcg catccgacag ttgcgcggta
    14101 ttttttctcg tcccaggtga tttccagtgt tttcatgcgt gggcaggggt tatcttgtcc
    14161 atatgttaaa taatgtcgtt tttttccttt tttctgaaaa aaccgacgga tttcgctcat
    14221 cgaaatgatt aataaggata cgtttccaat aaatactact cgcataaaca ggctgtgctt
    14281 ctgcagtctc gtttttatcg gaggatcatt atggctgttg aaaagcccca tcttaatctg
    14341 gcggtcatcg ggcacatcga ccatggaaag tcaaccaccg ttggcaggct catgttcgag
    14401 accggtgccg tacctcctca catcattgag ggttaccgca aagaggctga gaccaagggt
    14461 aaggcaacct ttgagtttgc ctgggtgatg gataacctca aggaagagcg tgagagaggt
    14521 atcacgatcg atatcgcgca caagaggttc gacaccccga agttctattt caccgtggtg
    14581 gactgccctg gacacaggga ctttgtcaag aacatgatca ccggtgcctc gcaggcagac
    14641 gcagcggttc ttgtcgtggc agcccctgac ggggtcatgg aacagaccaa ggagcacgtg
    14701 ttcctttcaa gaacgcttgg catcaaccag ctcatcgttg ctgtcaacaa gatggacgca
    14761 gccaagtatg accagaaacg cttcgaggaa gtgaagaagg atctctctgc cctcctccag
    14821 atggtgggat acaagccggc agagacgctg ttcatcccca tcagttccct tcagggggtc
    14881 aatatcgcca agaagagccc ggagacgccc tggtataccg gccccacgct tcttgagtcg
    14941 cttgacaccc tcaaggaacc cgagaaaccg accgacaagc cgcttcggct cccgatccag
    15001 gatgtctaca gcatcagcgg tatcggcacc gtgccggtag gacgcgttga gaccggcatc
    15061 atgaagaagg gaatgaaagt atccttcatg ccggcaaaca aagagggtga gatcaagtcc
    15121 atcgagatgc accacgagga gatccctcag gccttgcccg gcgataacgt tgggttcaac
    15181 gtccgtggcc ttgcaaaggg agacatccgc cgtggcgatg tctgcggacc tgtcgacgca
    15241 ccgccaactg tcgctgatga attcaccgca cagatcgtcg tgctccagca ccccagcgca
    15301 atcactatcg gatacacccc ggtgttccac tgccacacca cccagactgc ctgcaccttc
    15361 gtcgagctga agaagaaact cgacccaagg accggccaga ccaaggaaga gaaccccacg
    15421 ttcatcaagg caggagacgc agcgattgtg caggtcaaac cgaccaagcc catggtcatc
    15481 gagaatgtga aggagcttcc ccagctcggc aggtttgcaa tccgggatat gggaagcacc
    15541 atcgctgccg gaatgtgtat tgctatccag cccaagcaga tgagataact ccgaatccca
    15601 caatatttat ctggtgaata tcatgcagaa ggccaggata cgcctcacag ggaccgattt
    15661 caagaaggtt gagatggtct gcgaccggat acgggagatc gcagagagga ccggtgtcaa
    15721 catcgccgga cccattccac tccccaccaa acgcctggtg gtgcccatac ggaaaagccc
    15781 cgacggggaa gggaccgcca cctgggaccg gtggcagatg cgggtgcaca aacgactgat
    15841 cgatatcgac gccgatgaac gtgcactccg ccagctgatg cgtacccagg tcccgaaaga
    15901 tatcggcatc gagatcgtcc tcgagagctg aggaagcgga ttgaccgccg gagttcgctc
    15961 ccggatccgg gaatggtgct cctttcggaa cctcttttta ctgatttttc tcctcggtat
    16021 tcttctccgg ttcttatccc tggatctcaa actcttccat cacgatgagg cgatccatgc
    16081 gtggttctcg taccgtctga tgacagaggg agagatccag ggataagaac c
//
LOCUS       DAOOCO010000018        15514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3435_length_15514_cov_4.464066, whole genome shotgun sequence.
ACCESSION   DAOOCO010000018 DAOOCO010000000
VERSION     DAOOCO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3435_length_15514_cov_4.464066"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..654)
                     /locus_tag="PK154_02020"
     CDS             complement(46..654)
                     /locus_tag="PK154_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09870.1"
                     /translation="MNLLIIRRVEYAILGFFSLCFIYYAFQALIIGDTFRFIYVLIPS
                     LALSLLPVIFEFAMKVRFPPGFKLLFSLALLVHVAGGISRFYWKFAPFYDKIAHVCSA
                     LALFLLIISVFSFLDYHGTTLKWRTVLITTVALVTVLMITWEISEYVIDVIATTSYNN
                     GIIDSIGDVIANIIGIIIGFYIVRYYRTLIPDGKTLGSLFTL"
     gene            complement(749..1630)
                     /locus_tag="PK154_02025"
     CDS             complement(749..1630)
                     /locus_tag="PK154_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09871.1"
                     /translation="MSSLDEFLGPLHHGVWEVEIPIESVTEPLDQGWEVSRINIPSPG
                     TIASYRKGKYHVHETATQWRVHLDNHDPKHHPILHLIDDAPLLLMIGDTFGTLIATTR
                     KKTGNEAKILEGQSKAWKDQVLIGLFIILVGIFVVTNPLLTFKGITRLFVPLVIIGLG
                     IFTITKSVRVQPFRLNDDPVLYRGIIITTAGFIAFYLPLTLWIVGLLGILAVWMLATA
                     IVLLGRARKGRSAIPEGFVSRIVIAILSLCLVGLIFTYPIEIVGLLMILLGVIGLLLG
                     IMLFVNGVRLKRWMERT"
     gene            complement(1806..2222)
                     /locus_tag="PK154_02030"
     CDS             complement(1806..2222)
                     /locus_tag="PK154_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW09872.1"
                     /translation="MFCIFFLVIFAGCLAPISPTRAPPGQTWRLTSYNAGNKMIKPES
                     NTTITLAFGPDGLIRGSTVCNEYQATYQFEGEILSLGNIGSTDRNCTTPIKMTEMEKV
                     YFQLLNNTTRFSLDQNTLTLSYFDEKKLLVFERQPF"
     gene            complement(2500..3762)
                     /locus_tag="PK154_02035"
     CDS             complement(2500..3762)
                     /locus_tag="PK154_02035"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HPW09873.1"
                     /translation="MPEWGVYSEVGRLRTVMVHRPDLSLRRLTPANHDKYLFDDVIWV
                     ERAIQEHDTFVRLMKDEGVQVYYLQELLTKSLTADQKIREWVIDQAINERTVGISALD
                     AVRICLNDMTPADLASHLIGGLTVGELECIYMEGLSRFSLTAAASGPDAFILPPLPNT
                     LFTRDSSSWIYEGVTFNPMFWPVRRLEVMNVAAIYRGHPMFRDFPQLTWYPPPGYDNA
                     LRFEQWGPSSMEGGDLMPIGNGTVLVGMGERTQARLIEQLARVLFSHGAAERIIVAPM
                     SHNRAHIHLDTVFTMLDAGTVTVFPDVVDRMPAYSLQPGDEHALFTVTREESFLAAVT
                     DALGVDRLTVIPTGGDKYEAAREQWDDGNNILAIRPGVVIAYNRNTCTNQNFRDAGIE
                     VLECEGSELSRGHGGGHCMTCPLLRDPI"
     gene            complement(3755..4687)
                     /gene="arcC"
                     /locus_tag="PK154_02040"
     CDS             complement(3755..4687)
                     /gene="arcC"
                     /locus_tag="PK154_02040"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPW09874.1"
                     /translation="MRIVVALGGNALLKRGEPMTAEKQWENVRAAAMALAPLATENEL
                     VLSHGNGPQVGLLALQAACYTAVDPYPLDVLDAQTEGMIGYMIEQELGNLLPERIPFA
                     TLLTMVEVDPDDPAFSDPAKFVGPGYLESEADRIAREKGWVFKRDGDSWRRVVPSPRP
                     KQIFEIRPVKWLLEKGTVVICAGGGGIPVMFHQEKKRILTGVEAVIDKDLASALLARE
                     IGADLFVMVTDVRGAYLDWGTPAARLIEETTPSELCKYEFPAGSMGPKVEAACQFVRK
                     TGKRAAIGALEEIQAIVSGRSGTQVHPEGGHGHA"
     gene            4931..5560
                     /locus_tag="PK154_02045"
     CDS             4931..5560
                     /locus_tag="PK154_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09875.1"
                     /translation="MPSTRAVYIAWAMQGLILLEGMYGISIRDPSIIVTAFIGLFLTI
                     IPYLIERRIQVTLPWEVNVLIAFAVFLHVAGYSQQLYLLLYPYYDKFAHFVSSITVAV
                     LAFVSILLIDRFSCTKLARWQIFFYIVIFTMAIGAFWEIYEYLMDTFLLAYLTKPLQH
                     GLDDTMMDLMFDLLGGIIVGFFGTWYIQKKTLDELTNPMVGDSAPECKL"
     gene            complement(5989..7485)
                     /locus_tag="PK154_02050"
     CDS             complement(5989..7485)
                     /locus_tag="PK154_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HPW09876.1"
                     /translation="MAPASAVITGVNTISREGDVFIGEEGLDITGCMGNATLLAWFGP
                     GSGSTRDAPSYVIDIGDPTSFYIAPAIFINKTGPWYQWYGSPPAGPVAVNVLEPYLET
                     GVRSQATGGSVSYGNVPQGDYLNFFISSNMWTVSSRPGYDPSRDGFLSYKVKSPEGTI
                     YSGLHQNRTYVIPLTGISIDSTYETWVPAPPPYNENGWNTGVTDSSGTLVYGPGLYEV
                     TVETDLNGIKDNYRDPSGADYIGKTVAYPQLITIGSDTLTLTASDSTIIRGNQFSITL
                     RGMPSEVYIVWVENTGHMTGLPQDQTPTLRATQSGLRFDPVSGPYTFGGYQFQGGNDR
                     TVKEDVPTSPVNGTLYYGRVSLSSTGSRTIGWQTTSDTKDRTYTIRAERGPPGPNGLP
                     DIFSKVTDYVSAEVDIEIKKGSVTIVADRETSVPESEEVLPDITPITVSPTVHHETMS
                     TAIPETRISTVSTPFPTLPPVPTSSGGLGPGIVLASLVMLVLFFYRRS"
     gene            complement(7705..8220)
                     /locus_tag="PK154_02055"
     CDS             complement(7705..8220)
                     /locus_tag="PK154_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09877.1"
                     /translation="MRTAANQKQQSGEHSTGYKGLTVIIILCFLGIMPLLIWISLPET
                     STYAVVTTSSAMIQSAAAEAGLEICSQEAKSVEVQGAQSAILYQLAPDCSRSTDENRV
                     KILVVGFNNTEDQMAAIAEAQVTYSNWQTTNTAAFMSGTSTIIVHAAPGNQAVGQISS
                     SLIGQGAARIL"
     gene            8309..9481
                     /gene="thiI"
                     /locus_tag="PK154_02060"
     CDS             8309..9481
                     /gene="thiI"
                     /locus_tag="PK154_02060"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPW09878.1"
                     /translation="MDVVMIRYGELFLKSEPVKRHFIQTLTRNISKALQSMGLSHRFE
                     FHRGRLLIHGDDPLQIATVAARVFGIVEVAVCHQTSLSLDEIATEAVRVAAKRLIRGA
                     RFAVRARRQGVSGITSQEIAAGVGSVLCDAYPHSRVDLSHPEYEVFIEVRDFGALIYD
                     EPIPAPKGLPWGTQGRVLSLLSAGIDSPVATWLMMKRGCEVTSLHVDGGAYAGRDVRP
                     TAERHHAVLSGWCAGFPMELLVVDAEPFYDAIMKKGPARYRCVLCKRFMLGLGSAIVK
                     DRSFEALVTGDSLGQVASQTLTNMATISEAATVPLLRPLIGFDKEEVVGIARNIGTFD
                     TTQGDLACQAVPRTPATAARSKEIREAEALVGMNELISGAMDRLSIIQGLNGKIVP"
     gene            complement(9647..10936)
                     /locus_tag="PK154_02065"
     CDS             complement(9647..10936)
                     /locus_tag="PK154_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HPW09879.1"
                     /translation="MGIRFFRGEKIRFEHEYHQLKEIVELLRKEYQKEPVYILTNVLV
                     ANRQLDCVLLTRSGPIILELKAFSGRVHGLENGPWDVMTKDGNIPLPNLFVQAKLQRQ
                     DLIDRLIPIYDEHLPHINGNNLRKLRSWIYFSRGTVYPDGQIDFRRVKWFRIVNADSL
                     LETMRFPDSGYTLRIQDMDAIVNGLHLEEYELSNGHSVAPAPAETNQSTGLSKRLLAA
                     IVIGVVIGAIILLLFLVPGAKVAFLSSVNGIITVVGGLISQGSREFIKVESTPADSQE
                     AMIYLNQIRVSGGILPVPFDEQAYQLAIARAEDMATYRYLDYKNPETGSTAILMKSRF
                     GINPWDLIIECAYGQWNGYTFGIEKDAIDAWLTDEGNRDRLFSPIDRGAIACSHGYCS
                     FIGISPQPKSLTDKGEPVFVEQKEVQPEVNVTGTVAQ"
     gene            11134..12183
                     /locus_tag="PK154_02070"
     CDS             11134..12183
                     /locus_tag="PK154_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPW09880.1"
                     /translation="MKWKGWDHVRLDPETGAVRLQGSPAPLVDAIAPVIVSASRSTDI
                     PALYGDWFIERLKRGYVTWKSPFDGRIIPVSFVNTRVFVFWSKNPRPFFPQLADLTKD
                     GRQCLFLFTLNDYAREDLEPGIPLLEERIRTFAEISSLTGRGRLTWRFDPLLLSDSVT
                     IDELLERIGSIGDQIARFTERLVISFIDIARYPRVQRNLAKCGLSGIREFSSEEIRLF
                     ASGLSQLNEEWGLEILACGEEIDVTGYGIAHGECISLDQIAREFSSDQYLMDFLLPPL
                     ESATRERRKRELKDPGQRSRCGCVVSKDIGCYSTCIHGCRYCYATSSPSRAEKNYQIY
                     CEDKGRGIIHPGIIG"
     gene            complement(12173..13003)
                     /locus_tag="PK154_02075"
     CDS             complement(12173..13003)
                     /locus_tag="PK154_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPW09881.1"
                     /translation="MRRHPFLISIPHGGLRVPAEVSEMVGLTQAELVFYSDPATRALY
                     RFRERVSAFLDTDISRMIVDLNRAPYHLPPKHPDGVIKQKTVFGTPVYRNGSIPDIRI
                     VHQLLMEHYFPYHAAVDQYLDPGQIAFALDCHSMLPHGPPGHRDAGRERPLICLGNNG
                     DLHGEPRPGSLSTCPSAWIQALAAAFREEFGANGEVAINRPFSGGFITNAHFWHKGIP
                     WIQVEVNRDLYERGGADPAFSVINQENLNFTGERIWNALCSFWDGLETSRDSIEETIR
                     "
     gene            13131..13421
                     /locus_tag="PK154_02080"
     CDS             13131..13421
                     /locus_tag="PK154_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09882.1"
                     /translation="MKAPPHFIRNNEEWIIWLLEAEFSGSATPYALSSRTGISIDTIH
                     DNFLYMERVGLLSIERDPDKKYPDEIARVNLTENSRRVYDELKIRPDPGDLF"
     gene            13475..14041
                     /locus_tag="PK154_02085"
     CDS             13475..14041
                     /locus_tag="PK154_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09883.1"
                     /translation="MKQNRGYDATPDVSGMQRKMPVPDEMRWRIAANIAARIPLAYGM
                     VFREVAGADYDRLEQQIWVTLAYSVREVAWTYSLPAGDSRELISALEILNVIFFGPDF
                     KSEQISFEHDRSVLLIRKCPFLIRQQELSGPPDYAFNRCLAFSIALVEELNPLYTLRF
                     VRAMCQGDRNCEMKVMTRKDAEEAEKKG"
     gene            complement(14057..14542)
                     /gene="msrA"
                     /locus_tag="PK154_02090"
     CDS             complement(14057..14542)
                     /gene="msrA"
                     /locus_tag="PK154_02090"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPW09884.1"
                     /translation="MESGEKATFAAGCFWGIEAAFREVKGVISTRVGYTGGQAKNPTY
                     EEVCSGKTGHAEAVEVVFDPETVSYQVLLDLFWSIHDPTQLNRQGPDFGTNYRSAIFY
                     HTPAQKVLAEESRARIAESGRYGRRPVVTEIMPAGPFYPAEEYHQQFYEKQGRRSCRF
                     G"
     gene            complement(14734..15198)
                     /locus_tag="PK154_02095"
     CDS             complement(14734..15198)
                     /locus_tag="PK154_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPW09885.1"
                     /translation="MNPIEIASDAFREGERIPDEFTCKGANLSPPLSFGPMPPGTKSI
                     VIICDDPDAPSGLFTHWILYNIPAGTAHIPKGVPKKPVLIDGSRHGTNSYGRMEYSGP
                     ACPPGTTHHYHFDAYALDTVLALRAPVTRRDIGHAMEGHILGNGSLIGLFSR"
     gene            15301..>15514
                     /locus_tag="PK154_02100"
     CDS             15301..>15514
                     /locus_tag="PK154_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00289 family protein"
                     /protein_id="HPW09886.1"
                     /translation="MRLGVLCSGGKDSLFACMMAMEREEVVCLIALRSRNEESYMFHT
                     PNVSLVPLQAEAAELPLVEMPTEGMEE"
BASE COUNT         3693 a         3931 c         4127 g         3763 t
ORIGIN      
        1 ggattatctt aaaatttttg attggaacag aactgcacaa atagattaca aggtaaatag
       61 agagcccagg gtttttccgt caggaatgag ggtcctatag tatcgaacga tataaaaccc
      121 aataataatt cctatgatat tcgcaataac atctccaata ctatcgataa taccattatt
      181 gtatgaggtc gttgcgatca catcgatcac gtattcggaa atctcccatg tgatcatgag
      241 tacggtaact aatgcgactg ttgttatgag tacggtccgc catttcaacg ttgtgccgtg
      301 gtaatccagg aaagaaaata cggagatgat cagcaggaaa agggcgaggg cagagcatac
      361 atgggcaatt ttgtcataga agggagcgaa tttccagtaa aacctgctga ttccaccggc
      421 cacatgcacg agaagggcga gtgaaaacaa caatttgaat cccggcggaa accgcacctt
      481 catggcaaat tcaaagataa ccgggagaag ggacagcgcg agcgagggaa tcagaacata
      541 tatgaaccga aaggtatctc cgatgatgag ggcctggaaa gcatagtaaa taaagcacag
      601 tgagaaaaat cccaggatcg catattcaac ccttcggata atgagcaggt tcatcgcttg
      661 cttatacctg ttgtgtctcg gtgttaaaat agtaatcttt tcatttaagt gtgggatgtt
      721 tccatgatac cagatcgaat atcttttttc atgtcctttc catccatctc ttcaatctga
      781 caccatttac aaacagcatg atcccgagta aaaggccaat tacccccagc agtatcataa
      841 ggagtccgac aatttctatc gggtaggtga agatcagccc aacgaggcag agggagagaa
      901 tggcaatgac aatccggctg acaaatcctt cgggaatcgc ggaccttcct ttgcgggcac
      961 gtccgaggag aacgatggca gtggcaagca tccatactgc caggattcca aggagaccaa
     1021 cgatccagag ggtcagcggc aggtagaatg caatgaatcc tgcggtggtg ataattattc
     1081 cccggtacag gaccggatca tcattgagcc tgaagggctg caccctcacc gacttcgtga
     1141 tggtgaatat cccaagaccg ataatcacaa gggggacgaa gagccgtgta atacctttga
     1201 aggtcaataa cgggtttgtg actacgaaaa tcccgacaag aataataaaa agaccgataa
     1261 ggacctggtc tttccatgct ttcgattgac cttcaagtat ctttgcctcg ttcccggtct
     1321 tttttcgggt agtagcaatc agtgtcccga aggtatcgcc aatcataagg aggagtggag
     1381 catcgtcaat aaggtggagg attgggtggt gctttgggtc atgattgtcc aggtgcaccc
     1441 gccactgggt ggctgtttca tggacgtggt acttcccctt ccggtaactc gcaatggtcc
     1501 ctggagaggg aatgttgatc ctggatactt cccatccctg atcgagcggt tcggtcaccg
     1561 actctattgg tatctccacc tcccatactc catggtgaag gggtccgaga aactcatcaa
     1621 gactcgacat acagtgattt catttccata aaacgggtag gagtgtactt actcatatga
     1681 tatgtgaaat ggtgtcgtat tgttttcgcc atgagaattc cacgggtgag aaccggctga
     1741 tgagcttcca agagacccga tataattcgg catagtgtat tccgtttttc catgtggggc
     1801 agacgtcaga atggttgcct ctcaaagaca agaagtttct tttcgtcaaa gtaggaaagt
     1861 gtcagggtat tctgatcaag agaaaaacgg gtggtattgt tgaggagctg gaaatatact
     1921 ttttccattt ccgtcatctt tataggagtg gtgcaattcc tatctgtcga tccaatattg
     1981 ccgagagaga ggatctctcc ctcaaactgg tatgttgctt gatattcatt gcagacagta
     2041 cttcccctaa tcagtccgtc cggtccgaat gccagggtaa tagtggtatt ggattcgggt
     2101 tttatcattt tatttccagc attgtaggaa gtaagtcgcc aggtttgacc tggaggtgcc
     2161 cgagtggggg aaatcggagc aagacaccct gcaaaaatga cgaggaaaaa aatacaaaac
     2221 ataagagcag caagtctcgt gagcattggt actctaataa aattattact aggtctaata
     2281 atagtattgt tttttggaca tgctggatat tgaaactgga ttcgagattc gaatgcggtg
     2341 aataaatttc aggggaggat tggatgaaaa gacatgcatt gcagtcccga tccgattcga
     2401 tcttccataa acaggatttc gctggaaccg acatccagta ttatctgatt tgaaaatgcc
     2461 ttttcccgga acggtatacg tcacccttaa tccccttttt catatcggat cacggagcaa
     2521 cgggcaggtc atgcaatgcc ccccgccgtg tcctcggctt agttcggatc cttcgcattc
     2581 gagcacctcg atccctgcat ccctgaagtt ctggttggtg caggtattcc ggttatatgc
     2641 gattaccact cccggtctga tggcaaggat attgttccca tcgtcccact gctcccgtgc
     2701 ggcctcgtac ttatcgcccc cagtggggat gacagtaagg cgatcgactc cgagggcgtc
     2761 ggtgaccgct gcaaggaaac tttcctcccg tgtgacggtg aaaagtgcat gttcatcacc
     2821 gggctggaga gaatatgccg gcatccggtc aaccacgtca ggaaacacgg taacagtacc
     2881 cgcatcaagc atggtaaaga cggtatcaag gtggatgtgt gcccggttat ggctcatcgg
     2941 tgcaacgata atccgttctg cggcaccatg gctgaagagt acccgtgcaa gctgctcgat
     3001 cagtcgtgcc tgcgtccgtt cgcccattcc gacaagaacg gtcccgttcc cgatcggcat
     3061 cagatcacct ccctccatcg aagaagggcc ccactgctcg aaccgaaggg cgttatcgta
     3121 tcctggcgga ggataccagg tcaattgcgg gaagtccctg aacatggggt ggccgcgata
     3181 gatcgcagca acattcatga cctcgaggcg ccggaccggc cagaacatag ggttaaaggt
     3241 gaccccctcg tagatccatg acgaagagtc ccgggtaaag agcgtattgg ggaggggagg
     3301 gaggatgaat gcatcaggtc cggatgcagc tgcagtgagg gagaaccggg aaagcccctc
     3361 catatagatg cattcgagtt cgcctacggt gagtcccccg ataagatggc tcgcaaggtc
     3421 agcaggggtc atatcattca ggcagatccg gacagcatcc aacgctgaga taccaacagt
     3481 ccgctcattt atggcctggt caatcaccca ctccctgatc ttctgatcag cagtaagcga
     3541 cttggtcagg agttcctgga ggtagtatac ttgtactccc tcgtccttca tgaggcggac
     3601 aaatgtgtca tgctcctgga tcgcccgttc cacccagatg acgtcatcaa agagatactt
     3661 gtcgtgattg gcgggggtaa gccgacgcag gctgagatcg gggcgatgga ccatcaccgt
     3721 gcgaagcctc cccacttcgg aatacactcc ccactcaggc atgaccgtgt cccccttccg
     3781 ggtgcacctg ggtcccggat ctccctgaaa caattgcctg gatttcctcc agtgccccaa
     3841 ttgccgcccg tttcccggtt ttccggacaa actggcaggc ggcttccacc ttcggcccca
     3901 tagatccggc cggaaactca tatttgcaca actccgaggg ggttgtctct tcaatcagcc
     3961 gggctgccgg ggttccccag tcaaggtagg ctccccgcac atcagtcacc attacaaaaa
     4021 ggtcagcacc gatctcacgt gccagtaggg cgcttgcgag atctttgtcg ataacggcct
     4081 ccactccagt aaggattcgt ttcttctcct ggtggaacat cacagggata cccccacccc
     4141 cggcacagat gacgaccgtc cccttctcga gcagccactt gacgggacgt atctcaaaga
     4201 tctgtttcgg gcggggtgac gggactaccc tcctccagga gtccccgtcc ctcttaaaga
     4261 cccatccctt ctccctggct atcctgtcgg cttcagattc caggtaaccg ggacccacaa
     4321 acttggctgg atctgaaaaa gcgggatcgt caggatcgac ctctaccatg gtgaggaggg
     4381 tggcaaacgg gatgcgttca gggaggaggt tacccagttc ctgctcgatc atatagccga
     4441 tcattccctc agtctgtgca tcgagtacgt caagagggta gggatcgact gccgtgtagc
     4501 aggcggcctg gagggcgagg agcccgacct gggggccgtt cccgtgcgag agcacaagct
     4561 cgttttcggt cgccaggggg gcgagcgcca ttgcagccgc cctcacattc tcccattgct
     4621 tctctgcagt catcggctct ccccgtttca gaagggcgtt tccgccaagg gctaccacga
     4681 tcctcatacc tattctccca tacctggact ctgccacacg gtgtgcatca atgcagtata
     4741 ctgcagctaa ttaatatgaa attctcccct gaaaccttga tccgagatcc gctccaatta
     4801 acccaataca gaatccagtt gcgatacgga tcggtcttta ccctccctga aatcccttta
     4861 tttgccttga atgggggggt gggaagattc tgattaaaat cattatagtg ccagtaccag
     4921 atatataaca atgccttcca ccagagcggt ttacattgcc tgggcaatgc agggactgat
     4981 cctgcttgaa ggaatgtacg gaatttcgat ccgcgaccca agcatcatcg ttaccgcgtt
     5041 tatcggttta ttcctgacta ttatcccgta tctcattgag aggcggattc aggtcaccct
     5101 cccatgggag gtgaacgttc tgatcgcatt tgcggttttc ctccatgttg cggggtattc
     5161 acagcagctc tatctcctcc tttatcctta ttacgataaa tttgcacatt ttgtctcgtc
     5221 aataacggtt gccgtgctgg cgttcgtctc gatcctcctg atcgaccgtt tcagctgcac
     5281 gaaactcgcc cggtggcaga tcttttttta catcgtgata ttcacaatgg caatcggggc
     5341 cttctgggag atctacgaat atcttatgga cactttcctg ctggcctatc ttacgaaacc
     5401 tctccagcac gggcttgacg ataccatgat ggatctgatg ttcgacctcc ttggcggaat
     5461 tattgtagga tttttcggta cgtggtatat ccagaaaaaa acacttgatg aactgactaa
     5521 cccgatggtc ggggactcgg ccccggaatg taaactttaa aagggtggcc ggcctgcgtg
     5581 tgattcaaaa gaccattggt aaaagatttg atacccaggc tcatttgcct gtgatgaacg
     5641 tgtgtaagcg aaaggcctga agtggccttt ttccaatgaa aatcacttcg taattttcat
     5701 aatttatgcc cgtatcccga aatcggggct cataggaggt ttatatgaaa atgcatccgg
     5761 cattttcatc ttttatgctt gcgaatcctc aaaggatcat gttatttttt atgagaatct
     5821 cacgggaatt agctgcaggc tttcgtaaca gttgaaggat gctggaggtt cagagatgat
     5881 gctagcacgt tttttgtaac gggatgtgat cataagaaaa gagtactgtc agaaaagaat
     5941 tcctcctaaa ttcacgaaaa gtgagttccc aagagatccc tgctcacatc aagaacggcg
     6001 atagaagaac aggaccagca tgacaagact cgccaggact atacccgggc caagccctcc
     6061 tgacgatgta ggtacaggag gcagtgtcgg gaatggcgtc gaaacagtag atatccttgt
     6121 ttcaggaata gcagtgctca ttgtttcatg atgtacagtg ggtgaaacgg tgattggagt
     6181 tatatcaggt aatacttctt ccgattctgg tacactggtc tccctatctg cgactatagt
     6241 gaccgatcct ttcttgattt caatatccac ttcagcgctg acatagtcag tcaccttcga
     6301 aaagatatcc ggcagaccgt ttggacccgg tggtccccgt tcggcacgaa tggtataggt
     6361 ccgatccttc gtatccgagg ttgtctgcca tcctatggtc cgtgaaccag tactagagag
     6421 agagacacgt ccataataga gggtcccgtt cacgggcgat gtcggaacat cttccttgac
     6481 cgtgcgatcg tttcctccct ggaactggta tcccccgaat gtatatggcc cgctcaccgg
     6541 atcgaaacgg agaccactct gggtagcccg cagagtgggg gtttgatcct gcggcagacc
     6601 ggtcatatgc ccagtgtttt cgacccagac gatatagacc tcgctcggca tccccctgag
     6661 cgtgatcgag aactggttgc cccgtatgat tgtggagtcg ctggcagtga gggtcagggt
     6721 atcagaaccg atagtgatga gctggggata ggcgaccgtc tttccaatgt agtcagcccc
     6781 cgacggatcc cggtagttgt cttttatgcc gttaagatct gtttcgaccg tgacttcgta
     6841 gaggccgggg ccgtatacca gggttcctga tgaatcggtc acccccgtgt tccatccgtt
     6901 ttcattatac ggaggaggtg ccggaaccca tgtctcgtag gttgagtcaa tgcttatgcc
     6961 agtaagtggg ataacatacg tccgattctg gtggagccct gaatagatgg tcccttcagg
     7021 ggatttcacc ttgtaggaga gaaatccgtc ccgggaggga tcatagccag gccgggagga
     7081 gaccgtccac atgttgctcg agataaaaaa attcaggtaa tcaccctgcg ggacgttccc
     7141 ataggagacc gaacctcccg ttgcctggga gcgtactccc gtttccaggt aaggttcaag
     7201 gacattcact gcaaccgggc ccgcaggagg agaaccgtac cactggtacc agggccccgt
     7261 cttgttaatg aaaatggctg gagctatgta aaatgacgtg ggatcaccga tatcgattac
     7321 ataagaagga gcatcgcggg tgctgccgga gccgggtcca aaccacgcaa ggagggtcgc
     7381 atttcccatg caaccggtga tatcaaggcc ttcttcccca atgaagacat ccccttccct
     7441 gctgatagta ttgaccccgg taatgacagc agacgccggg gccatgacta ccatcaagaa
     7501 agcgatggta agtaatcctt tgcggcacag cttgtacatc tccagcatcc tatcctcaaa
     7561 acgtatacaa taaggcccag gtatataatc ccggcggtct tctctgtcaa acaagaaagg
     7621 aatatgttcg gcgatccctt ccttttggtg agcaaagcat cattcggatg cgttctgaca
     7681 tgaaaaaaag gatgagacgc ggttttacag gatccttgcc gcaccctgtc cgatcagcga
     7741 ggaactgatc tgcccgactg cctgattgcc aggggccgca tgcacgataa tggtactggt
     7801 gccgctcatg aaagcagcgg tattggtcgt ctgccagtta ctgtaggtca cctgtgcttc
     7861 tgctatggcg gccatctggt cttccgtgtt attgaatcca acgacgagaa tttttaccct
     7921 gttttcatcg gttgatctgc tgcagtccgg ggcaagctga tacaggatag cactttgtgc
     7981 tccctggacc tcaacgctct tggcttcctg tgagcagatc tccagccctg cctctgcagc
     8041 agcggactgg atcatcgcag aagaagtggt gaccaccgca taggtgctgg tctccggaag
     8101 agaaatccag atcaggaggg gcatgatccc gagaaaacag aggatgatga tcaccgtcag
     8161 tcccttatat ccggttgaat gttcgccaga ctgttgtttt tggttggcgg cggttctcat
     8221 ggtctcgaag agtattactg gtttcctgat ataagggtaa tggtttttga atcttgccgt
     8281 atcatgttgt acatcactgg agcagggtat ggacgttgta atgatccggt atggagaact
     8341 ttttctcaag agcgagcctg tgaaacggca tttcatccag accctgacac gaaatatcag
     8401 caaggcgctc cagtccatgg ggttgtccca tcgttttgaa tttcaccggg ggcgcctcct
     8461 catccatggt gatgatccat tgcagattgc aaccgttgca gccagggtct tcgggattgt
     8521 tgaggttgct gtctgtcacc agacctctct ctctctcgat gagatcgcta cagaggctgt
     8581 gcgggtggcc gcgaaacgac tcatcagagg ggcgaggttt gcggtcagag cacgcaggca
     8641 aggggtttcc gggatcacca gccaggagat agcagccggt gtgggatccg tcctttgtga
     8701 tgcctatccc cattccaggg tagatctgtc gcatcctgaa tacgaagtat tcatcgaagt
     8761 gcgtgacttc ggtgcgttga tctacgatga gccgataccc gccccgaaag ggctcccatg
     8821 gggaacccag ggaagggtgc tctcgttgct ttctgcaggg atcgactctc cggtcgctac
     8881 ctggctcatg atgaagcgag ggtgcgaagt gacatcgctt catgttgacg gaggggcata
     8941 tgccggcagg gatgttcgtc cgactgctga acgccaccat gcagtccttt caggctggtg
     9001 tgcaggcttt cccatggagt tgctggtggt ggatgcagag cccttttatg acgcaatcat
     9061 gaagaaagga ccagcacggt accggtgcgt tctgtgcaag cgcttcatgc ttggcctggg
     9121 gagtgcgata gtcaaagacc gatctttcga agcattggtc accggtgaca gccttggcca
     9181 ggttgcttcc cagaccctga ccaacatggc aaccatctcg gaagcggcaa cagtgcctct
     9241 gctgcgaccc ctgatcggct ttgacaagga ggaggtggtc ggtatcgcac ggaatatcgg
     9301 gacttttgat accactcagg gcgatctcgc atgccaggca gtgccgcgaa cgcctgcaac
     9361 cgccgcccgc tccaaagaga tccgggaagc cgaagctctt gttggaatga atgagctgat
     9421 atcaggtgcc atggaccggc tgtccatcat ccaggggctc aatgggaaga tcgtccctta
     9481 aatcattgag ggtacctcat ctgtcgcggg aagccctgca atccatgttc ttgatactat
     9541 cgaaaagcgg gggggtgatg tatttccgct gcgaatgctc cccttgcaat ccttcgtatt
     9601 ttaattgaag catcggcaga acatcaggaa ttatacgatt tacctgtcat tgggcgacag
     9661 tccctgtgac gttcacttct ggctggactt ctttctgctc gacaaaaacc ggctcccctt
     9721 tgtcggtaag ggatttgggc tgcggggaaa tcccaataaa agagcagtac ccgtgagaac
     9781 aggctatggc tcccctgtcg attggtgaga agaggcggtc acggtttccc tcatcagtca
     9841 accaggcatc gatcgcatcc ttctcgatgc caaacgtata gccattccac tgtccgtacg
     9901 cacattctat gatgagatcc cagggattga tgccaaatct ggatttcatc aatatcgccg
     9961 tgctccctgt ctcaggattc ttgtaatcca gataccggta ggtggccatg tcttctgccc
    10021 tggcaatagc cagttgatag gcctgttcat cgaaaggaac aggcaggatt ccccccgaga
    10081 cacggatctg gtttaagtat atcatcgcct cctgtgaatc tgcaggggta ctctccacct
    10141 tgataaattc acgggaaccc tgactgatca gccccccaac aacagtaatg ataccattga
    10201 cactggaaag gaatgcgacc tttgcaccgg gaaccaggaa aagaagcagg atgatggctc
    10261 cgataacaac tccaatcacg atcgcagcga gcaatctctt gctcagcccg gtgctctgat
    10321 tcgtctcagc aggtgctgga gcgacagaat ggccattact taattcatat tcctcgagat
    10381 ggagcccgtt tacaatcgca tccatgtcct ggatacggag cgtatatcca gaatcaggaa
    10441 acctcattgt ttcgaggagg gagtcggcat tcacaatccg gaaccatttc accctccgga
    10501 aatcgatctg accgtcagga tagacagttc cccgggagaa atagatccag gatctgagct
    10561 tcctgaggtt attgccattg atatgaggga gatgctcatc gtaaatgggg ataaggcggt
    10621 cgatcaggtc ctgtcgctgg agttttgcct gcacaaaaag gtttggcagg gggatattcc
    10681 cgtcctttgt catcacatcc cagggcccgt tctcgaggcc atggactctg ccggagaatg
    10741 ctttcagctc gaggattatg ggacctgacc gcgtgaggag tacacaatca agctgcctgt
    10801 tggcgactaa gacattcgtg agaatgtata ccggctcttt ctggtactct ttcctgagta
    10861 gttcaacaat ctccttcagc tggtggtact catgttcgaa ccggatcttc tccccacgaa
    10921 aaaaacggat acccatttgt aactgttcct gcgctccctt ttccttatgt tctatattgc
    10981 ttcctttttc attaaactga tcgtctccaa ctaaaaagga ggcatggagg ccggtttctc
    11041 tggatggcag aagcaatgac gcggatgcac ggcagttatt atcatccctg agccgtgatc
    11101 aatgagaaag atcaatcagg aggagaaagg tgcatgaaat ggaaaggctg ggaccatgtg
    11161 cggcttgatc cggaaaccgg cgctgtgagg cttcagggtt cacccgcgcc actggtcgat
    11221 gcaattgccc cggtcattgt ctcggcaagc cgttccactg atatcccggc actatacggg
    11281 gactggttca tcgagcgcct gaaacgaggg tatgtgacat ggaagagccc gtttgatggc
    11341 cggatcatac ccgtctcttt cgtcaacacc cgagtgtttg tgttctggtc gaagaatccc
    11401 cgcccgttct tcccccaact tgccgatctc accaaagatg gacggcagtg tctctttctc
    11461 tttaccctga acgactatgc aagggaggat cttgaacccg ggatccctct gctcgaagaa
    11521 cggatcagga cctttgctga gatctcctcc ctgaccggac gaggcagact cacctggcgg
    11581 tttgatccgc tcctgttgag tgattcggtc acgattgacg aactgctgga aaggatcggg
    11641 agcatcggcg atcagattgc acggttcact gaacggctgg tcatcagctt tatcgatatc
    11701 gccaggtatc cgcgagtgca gaggaacctt gcgaagtgtg gactctccgg aatcagggaa
    11761 ttctcatcag aagagatacg gctgtttgca tcagggcttt cgcagctgaa cgaagaatgg
    11821 gggctcgaga tactggcctg cggcgaagaa attgacgtta ctgggtatgg cattgctcat
    11881 ggcgagtgca tctcgcttga tcagatcgcc agggaattct caagtgacca gtatcttatg
    11941 gacttcctgt tacccccgct cgaatctgca accagagaaa ggcgaaaacg cgagctgaag
    12001 gatccaggtc agagaagcag gtgcggatgc gtggtgagta aggacatcgg ctgttattcc
    12061 acgtgcatcc atgggtgccg ctactgctac gcaacctcat cgccttcccg tgcagagaag
    12121 aattaccaga tctactgcga ggacaaggga aggggcatta ttcatcctgg catcatcgga
    12181 tagtctcttc aatggagtct ctggaagtct ccagaccatc ccagaatgaa cagagggcgt
    12241 tccagatgcg ctcaccggtg aagttcaggt tttcctggtt gatcaccgaa aaggccggat
    12301 ccgctccgcc ccgctcgtaa agatcccggt tcacctcgac ctggatccat gggatccctt
    12361 tatgccagaa gtgagcattc gtgataaatc ccccggagaa cggacggttg atggcaactt
    12421 ctccatttgc gccgaattct tcacggaatg ccgcggcgag agcctggatc catgcggaag
    12481 ggcaggtaga gagacttcct ggccggggtt ctccatgaag gtcaccatta tttccaaggc
    12541 agataagagg acgctctctc cctgcatccc tgtggccggg aggaccatgc gggagcatgg
    12601 agtggcaatc cagtgcgaaa gcgatctggc ctggatccag gtattgatcg acagctgcat
    12661 gataggggaa gtaatgctcc attaatagct ggtggacgat tctgatatca ggaatggatc
    12721 cgtttctgta taccggggtt ccaaaaacgg tcttctgctt gatcacccca tcgggatgtt
    12781 tcgggggaag atggtacggg gctcggttca gatccacgat cattctcgag atgtctgtat
    12841 caagaaaggc ggacacccgt tcacggaacc ggtataacgc ccgggttgcg ggatcactgt
    12901 agaaaacgag ttcggcttgt gtgaggccta ccatttcact cacttctgcc gggactcgta
    12961 gtcccccgtg aggtatagag atcaggaatg ggtgtcgtct catttagtgt acggatttgg
    13021 gacgccggaa gatgaacgtt cgggtattcc tgtccctgcg tgatgatcac gaactttatg
    13081 gtgctttcac ccaaaataaa ggatcaaccg tcctgtgttg aggcaaaccg atgaaggcac
    13141 caccacattt catacggaac aatgaggaat ggatcatatg gctcctcgag gctgaattct
    13201 caggatctgc aactccctat gccctctctt cccgtacagg gatatccatt gacaccatcc
    13261 atgacaactt cctctacatg gagcgggtgg gcctgctctc gatcgaacgt gaccctgaca
    13321 aaaaataccc ggacgagatc gcgagggtga acctcaccga gaacagccgg agggtctatg
    13381 acgaactgaa aatcaggccg gatcccggcg acctcttctg aaccgggatt ctggaaggag
    13441 atcctgttgt ttttagggat cccatcgtct ccggatgaaa cagaacaggg gatatgatgc
    13501 gacaccagac gtgagtggta tgcaaagaaa gatgcctgtc cccgacgaga tgcgatggcg
    13561 gatagccgca aacatcgctg cccggatccc gctcgcctat ggcatggtgt tccgggaggt
    13621 tgccggtgct gattacgacc ggctggagca gcagatctgg gtcacccttg cctatagtgt
    13681 aagggaggtg gcctggacct attcgcttcc cgcaggggat tcacgggaac tgatctcagc
    13741 gctcgagatc ctgaacgtga tattctttgg cccggacttt aagtccgagc agatctcgtt
    13801 tgaacacgat cgttcggttt tgctcatacg gaagtgtcct tttctgataa ggcagcagga
    13861 actgtccgga cctcctgatt acgcattcaa ccgctgcctt gccttctcta tcgcgcttgt
    13921 ggaggaattg aatcctctgt acaccctccg gttcgtccgg gcgatgtgcc agggggaccg
    13981 gaactgcgag atgaaggtca tgacaagaaa ggatgctgaa gaggcagaaa aaaagggata
    14041 aagcactgag agacgactat ccgaacctgc agctccttct gccctgtttt tcgtagaact
    14101 gctggtggta ctcttcggca gggtaaaatg ggccggcagg cataatctcc gttaccacag
    14161 gacgtcgccc gtaacgacct gattcagcta tccttgctcg tgactcctct gcaaggacct
    14221 tctgtgcggg ggtatggtaa aaaatcgcag accggtaatt ggtcccaaaa tcaggtccct
    14281 gccggttgag ctgggtagga tcatggatcg accagaacag gtccaggagt acctggtatg
    14341 aaacagtttc aggatcgaat acaacctcaa ctgcctcggc atggccggtc ttccccgagc
    14401 atacctcttc gtaggtcgga ttttttgctt gtcccccggt atatccgacg cgtgttgaga
    14461 tcacgccctt cacctcccgg aatgctgcct caatacccca gaagcatccg gcagcaaagg
    14521 ttgccttctc tccactctcc atagtatcgc atgagcactc gtctggtatc aaggtgtgtt
    14581 tgggggaacc gggaaaagtg ttccggttgg gcaccaggaa tcctcctgga acagagaggt
    14641 tcccttttag taaaatgcct cattagaaaa aatgcggatg ctttttttcg ccgaaaaggg
    14701 gaatatcctg gtcatcctgt ggaacctgga cgattatcgt gagaacaatc ctatcagcga
    14761 accgttcccc agaatatgac cctccattgc atgcccgata tccctccgcg taaccggtgc
    14821 ccgcaacgca aggactgtat ccagtgcata tgcatcgaag tggtaatggt gggtagtccc
    14881 cggtggacag gcagggccgc tgtattccat tcgtccgtag ctgtttgtac catgccggct
    14941 tccgtcgatc agcaccggtt ttttgggcac accttttggt atatgggctg ttccggcagg
    15001 tatgttgtac aggatccagt gggtaaagag tccggaaggg gcatctggat catcgcagat
    15061 gatcacgatg ctctttgtcc ccgggggcat cgggccaaat gatagtggcg gtgagaggtt
    15121 cgcaccttta caggtaaatt catccgggat ccgctccccc tccctgaaag cgtccgaagc
    15181 gatttcaatc gggttcataa tagataccta tagtgatggt tacaggtact ctgaccacta
    15241 aaatgttatg gccctcgaaa acccaccagt taggtgaagc atggcagcgg aggaggggcg
    15301 atgaggcttg gggtactctg ttctggtgga aaggattcgc tgtttgcgtg tatgatggca
    15361 atggagaggg aagaggtggt ctgcctcatt gcactcaggt ctcggaacga ggagagttac
    15421 atgttccata ctccaaatgt atccctcgtc ccgctccagg cagaagccgc agaacttccg
    15481 ctggtcgaga tgccgacgga aggaatggag gagg
//
LOCUS       DAOOCO010000019        15433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3458_length_15433_cov_5.322279, whole genome shotgun sequence.
ACCESSION   DAOOCO010000019 DAOOCO010000000
VERSION     DAOOCO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15433
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_15433_cov_5.322279"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PK154_02105"
     CDS             complement(<1..1603)
                     /locus_tag="PK154_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPW09887.1"
                     /translation="MRWINPYAVVAGVVNRFPRAVLVTMVALAIVAVVFMGAIPPYTI
                     SDEYMDKSAPEGVVFDTYSNRYMQDTYILLIHAGDITDPELLSDLLILEEQIRRIDAV
                     SSTTTVADVVAQSNGGVIPGTRAEVQAIIDSLPPDMRQQFVPDPQTLFGYILIDQGLP
                     TDKSFTIEPVVVGTIAQATLPPGVSIEVTGNTPLNYEIFVAMMIGFVLLVIAAMVLML
                     VMKYLLWSKIRYWILPVVLLTFGLFYTFGLMGIFGIPANDGAIAAFPILLGLGIDYAV
                     QFHSRFDDECRVRDRKEALSLTITAAGPAVLTALVATSLGFLAMFISPIPMIRTFALV
                     SILGIVCCYLTALFGFGAFAHVLGYVPKAPGNTIADRIMTVYDTGLTAISSGVVKIAV
                     PIVLVAVVIALVGISVDPGIPVDASQQSFAPSDMTAVVVADEVESIAGSLTPLPLYVQ
                     GLDPRTIPGITWTDRLCGLLEENYDKISSIDSMSTLVRSYNNGVLPINQAELDRVLAS
                     IPPDQIIPYQVDDTTSVIRINTLAME"
     gene            1735..2331
                     /locus_tag="PK154_02110"
     CDS             1735..2331
                     /locus_tag="PK154_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW09888.1"
                     /translation="MQNDHFPLNTPFKPSPSLVTWFIIDFLFLMVFLVIVIPFSMYLS
                     SAIDTGIFFGIIGGMVVIVILFALWTKLYYESMWYELHEDEMRWKRGVIFRRTGIVPY
                     NRITNLDIRQGPVMRRLGISTISIQTAGYSAQAQAEIRIEAIVHAEELRELIRSMVRA
                     TIGGDGTGTGGTVPSVKTTEVQMLDELKAIRALLEKKS"
     gene            2511..3107
                     /locus_tag="PK154_02115"
     CDS             2511..3107
                     /locus_tag="PK154_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09889.1"
                     /translation="MKTSGCRESSAIKALISGLIGILFFLIFLVILGYIADHVSWPLL
                     SGFVDLLYANAALIIIFSVLFMIGEIFAAIRFPFNLPFPLFNAVASVLLVSFILAIMA
                     FIDSFYSLGIGSTLQFVKLILYPLTFIVVLITGYRSIFSKPEEKSPEDSAPVPDLSDK
                     EEERKISPSWEEIGGEFRQAVYDLIRRIRDELNLKERQ"
     gene            3209..3436
                     /locus_tag="PK154_02120"
     CDS             3209..3436
                     /locus_tag="PK154_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HPW09890.1"
                     /translation="MATRTISISEEAYERLKRLKKNEKMSFSDVILENFPKRRTLSEV
                     MSELGDCSELADSIEMASREIRKSRFREVRV"
     gene            3420..3827
                     /locus_tag="PK154_02125"
     CDS             3420..3827
                     /locus_tag="PK154_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPW09891.1"
                     /translation="MKSGYEMTVADTSFIIDLMRKDPGATMIFEHYEQAGRGLDTTGI
                     TALELYKGAYQSVNRDSLEKVKNILSLFSILPIDEQVYELFGKLSTDLRIRGTPLGAF
                     DEVIAAIVLCNDGEIISRDRHFGSIPGITVIPY"
     gene            complement(3897..4139)
                     /locus_tag="PK154_02130"
     CDS             complement(3897..4139)
                     /locus_tag="PK154_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09892.1"
                     /translation="MAARSWKEAKEIADREGAELVFHNYDKKEYGACSSDTTFGCFVK
                     GEFVEERCICMPAKFSPEELEKKEQAFLTENPDWGE"
     gene            4438..6012
                     /locus_tag="PK154_02135"
     CDS             4438..6012
                     /locus_tag="PK154_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPW09893.1"
                     /translation="MKKQKFIDLKAIAWDAMRKYGFEPGFSKAVMREVESIDAKNDPD
                     GGTNVRDLRPLLWSSIDNYDSMDLDQIEYCERGPGDEIHVMVAIADVDHFVPKRSQTD
                     HRAAHNGTSVYTGVETFPMLPDRLSKGISSLLPGNDHRAIVIEYTVLPDGDVREEGIS
                     RALVSNKAKLIYEEVGEWLDGQRTIPPSVSAVPGLEEQIRLQALATTRLKKYRMKQGA
                     LELETIEADAVAENGIVRDIVVQKMNAARFIIENFMIAANGTMVDFLGKAGIPMIQRV
                     VRVPRYWDEIVMTAAAYREKLPREPDSKALSLFLMKRKEADPERFPDLSLTIVKLLGP
                     GEYMTLEPGEPPTGHFGLAVTDYTHGTAPNRRYVDLVNQRLVKSVIDGKESPYTTHEL
                     YDLSTWLSDREKGSKKVERFMRKAAAAVLLKDRIGEEFEALVTGVTEHGIYARMISPP
                     AEGRILQGEQGLRVGQKITVRLLRTDPYNGFIDFACVRRGESGQGIYVERAPRSRASA
                     PVPRRSGYGRKQRSRR"
     gene            6097..6378
                     /locus_tag="PK154_02140"
     CDS             6097..6378
                     /locus_tag="PK154_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09894.1"
                     /translation="MFIMPDFIHTPEEWIVWLLGSEFHESASVDMLVQRTRYRREDML
                     SFIGVLERMKAIRVNRNARDAVIIDSVALVPNGKRLYGELQQRSGQQPG"
     gene            complement(6393..6599)
                     /locus_tag="PK154_02145"
     CDS             complement(6393..6599)
                     /locus_tag="PK154_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09895.1"
                     /translation="MCPDQKSIEKKICDVCGREAIGMQILGCCASTVCEEHAEQQLRG
                     LSPGEKLQWGVCYFVRFPEDPDEK"
     gene            6784..7356
                     /locus_tag="PK154_02150"
     CDS             6784..7356
                     /locus_tag="PK154_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPW09896.1"
                     /translation="MSPPLLSLTLPNHNEQDPFHRIIYRSGNNPPFMRTNRHQYFLDL
                     ALRCAHQGTCLRRNFGAIIVDEYNTIVSTGYTGAPRKQVDCTELNRCWRNDHNIPSGS
                     NYEKCRSVHAEMNALLQAGKLARGCTLYLAGYEVETEALTQIWPCFLCSKMIVNAGVR
                     NVIMRTGETTYEEMDPIVLYQKRSREALGE"
     gene            complement(7498..9990)
                     /locus_tag="PK154_02155"
     CDS             complement(7498..9990)
                     /locus_tag="PK154_02155"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPW09897.1"
                     /translation="MEFERLGAFYLGKEYSLKEKKLLDRLVMYDSRDLTTHAICIGMT
                     GSGKTGLCVDLLEEAAIDGVPAIIIDPKGDITNRLLMFPDLAPADFLPWINPDDARRK
                     GKSPDEFAASQAESWKSGLASWGQDGARIRMLRDAADLVVYTPGSEAGVPVSVIQSFA
                     APPLSWDSDAELIREKISGTVTAILGLIKVAADPLSSREHILLSSLFEQFWRNGKDLD
                     LVTLIQAIQNPPLKQIGAFDIETFFPAKERFALAMRLNHIIAAPSFQSWMNGQPLDIP
                     GFLATKEGRPRHSIFYIAHLNEDERMFFVTLLLHQVLAWVRSQTGTTSLRAILYMDEI
                     FGFFPPVANPPSKEPMLTLLKQARAFGLGVVLTTQNPVDLDYKGLSNTGTWFIGRLQT
                     VQDRERVLDGLEGAVQGGSFSRATLSDTLSSLDNRVFLLHNVHESAPVIFQTRFAMSY
                     LRGPLSRDQVRSLMKGRTPDRAMSTVRQQDAVATAASVPLSADTTPLLPAIPPEVPQR
                     YLPVRVPRETAETAILRSRPGKVEAASVLYEPALLASGRIHYTSTKMEKDAERDFALL
                     APFSAAGGVTWESAVPYPGLGTDLSSAVPPEARYTVPAEMVRALKKFSTEKQALTTFL
                     VHSRELNLSSCPAVKLISHPGEPETEFLLRVRLAARERRDAEVDALRKKYEAKIETLE
                     DRLRKAEMAADRKQADADARKREAMISAGETVIGVLLGRKSTRSGSAAASKYRQSSSA
                     GMSAREAEENARALEDDLRELKDEFEKESAAITALWENAVKEREEIVVKPKKTGIEIV
                     SFFLAWVPRWQITTRDAAGTTRTERVDASTGQ"
     gene            complement(10112..11635)
                     /locus_tag="PK154_02160"
     CDS             complement(10112..11635)
                     /locus_tag="PK154_02160"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HPW09898.1"
                     /translation="MEGPLERIEKEHGTLSTLQKILLSTDGSVTSLLEAIEGEEVTIR
                     TLSQNVGPADTKTAEELEIRPGDQVNHRIVELMNSRTREVLIYAVSDTPLERLEPGFR
                     SDLMRADIPIGRILKKHAIESRREISQVGVRGSDARISRIFGIALNNPVLFRKYRIIR
                     QGKPFISIEEVFPDCSFRMGAGVLVSAPSRLHLGLIDLNGSLGRIDGGIGITLSLPRT
                     VITAEQSPDLVISGGTSPYARRAGDTAHMVLSSLGMCGGARIHIRAVPPGHVGLGSGT
                     ALSLSVARALCELYGMPVPVRDLAVMTGRGGTSGIGTAAFESGGLIVDGGHSFGQGKE
                     KQEYRPSSASGGIRPAEVITRHDFPDDWQILLVIPEHDTPVSGAMETDIFRESCPVPV
                     DEVREICHEVMMRLLPGVVEHDLDLFGAAVNRIQEIGFKRIERERQPRTIRELPSVLC
                     EAGAACAGMSSFGPAVYAITDSGLAGLEAAVNGLLGDREATIIRTRADNHGALIRQL"
     gene            11908..13035
                     /locus_tag="PK154_02165"
     CDS             11908..13035
                     /locus_tag="PK154_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPW09899.1"
                     /translation="MHPKYIHTIDELDNLVGLPGKEKREMEQVCQTFPFRANDYYLSL
                     IDWNNAHDPLRKIVIPDPRELKGGGSLDPSHEKDYTKLPGLQHKYQPTGLLLLSDVCG
                     GICRFCFRKRLFVNCERETVKDVSQGLEYIRTHPEITNVLLTGGDPLILETSYLERIL
                     KELREIPHVNIIRIGSKVPAYNPFRILSDPHLSEVLSRYTTVEKRIYLMAHFNHPNEL
                     TTQSLQALEQLHRAGVIVVNQTPVLNEVNSSPETFTTLFRKLSFSGVTPYYVFQCRPS
                     LGNRLFQVPVEQSYHVIQQAWQACSGLAKRARFVMSHATGKIEVVGRQAGCVFMRYHQ
                     AAEKALIGKFMVMKSNPSAVWFDDYREIPLEKPVPRKVWLF"
     gene            complement(13114..14160)
                     /locus_tag="PK154_02170"
     CDS             complement(13114..14160)
                     /locus_tag="PK154_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HPW09900.1"
                     /translation="MKRVVIEVPNDKAEKIHDKFRDTIFSIHEGEASTRFILYVPDEM
                     LDDLIHKTNGSVKDTYPTRPLWTGFIPDYESLAKEDDRSILIEVSTPDFVISPFVERL
                     KEKLGYTKKQKRDEKTPIEKILASTESNTRFDQEKFILAAIAGIVAMIGLFLNNAGII
                     IGAMLISPLLGPIYALAVYVAVGDVKTTVRCVEILGLMVLMLVFIAAVASFALSFVID
                     LTLTPEILSRTDPNAVFILMAVLLGFATMIALSEGIPERIAGVAIAAALLPPAVVTGI
                     SLALFPAGAVKAIVLTLQNVIGLIAGSIIGVIFLHIGPRDIFAQIQSRQVIIRVVWFL
                     AILILFLMIISFLV"
     gene            complement(14414..>15433)
                     /locus_tag="PK154_02175"
     CDS             complement(14414..>15433)
                     /locus_tag="PK154_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW09901.1"
                     /translation="LAIRAGQDITVAPPRSTRFEPGQELAFLGSDEDFKRFLEDSGCV
                     QSGDRSILGEILNGEGFGFAELIVRPRASIIGKTPQEILFRQTFSIEPILLQRGETET
                     RGDLSGLSLAAGDLIVAFGSWDTLRHLARDRDLLLISPPEGEGVRYRKGPLAVLIFAF
                     SIALTLTGVPLSLAFLTGTAAMMLSHVLTIDEAYHAIEWKTVILIAGLIPLGIAIEKS
                     GAAALLASSLSAYLSGTHPLVVLVAIAIVATLLSLVISNVAATVLLVPIAMVTGAGTG
                     IDPRALALLVGVCASNSFILPTHQVNALLMGPGGYTTRDYLKAGGIMSVIFIAVAVIL
                     IFLLF"
BASE COUNT         3453 a         4289 c         4357 g         3334 t
ORIGIN      
        1 tctccatcgc gagcgtgttg atcctgatga cagaggtggt atcatcgacc tggtacggaa
       61 tgatctgatc aggcgggatg gacgcgagca cgcggtcgag ttctgcctgg tttatgggga
      121 gaacgccgtt gttgtacgag cggacgaggg tagacatgct gtcgatgctt gagattttat
      181 cgtagttttc ctccagaagg ccgcacaaac gatcggtcca cgtgatccct ggtatcgtcc
      241 ggggatcgag gccctggacg tagagcggca gaggggtgag cgatcctgca atcgactcga
      301 cctcgtctgc aacgaccaca gccgtcatgt ctgacggtgc aaacgactgt tgcgaagcgt
      361 cgactggaat gccggggtcg accgagatac ctaccaacgc aatgacgacc gcgacgagca
      421 caatcggaac ggcaattttc acgaccccgg atgagatcgc ggtaagaccg gtatcataga
      481 cggtcatgat gcggtctgca atggtgtttc cgggggcctt cgggacgtac cccaaaacat
      541 gggcaaatgc cccgaaccca aaaagcgcag tgaggtagca gcagactatc ccgaggatgg
      601 agaccagcgc gaaggtccgg atcatgggga tcgggctgat gaacattgcc aggaacccga
      661 ggctcgtcgc cacgagagca gtgaggactg caggacccgc cgcggtgatc gtcagtgaga
      721 gcgcttcctt ccggtcgcga acgcggcact cgtcgtcgaa ccgcgagtgg aactggacgg
      781 cgtaatcgat gccgagcccg aggagaatgg ggaatgccgc gatagcccca tcgttcgcgg
      841 gtatcccgaa gatgcccatg agaccgaagg tatagaacag cccgaaggtg aggagcacga
      901 ccgggagtat ccagtatcgt atcttggacc agaggaggta tttcatgacg agcatgagca
      961 ccatcgcggc gatgaccagg aggacgaagc cgatcatcat cgccacgaag atttcgtagt
     1021 taagtggcgt attgccggtc acttcgatag atactcccgg cgggagggta gcctgcgcaa
     1081 tggtaccgac aacgaccggt tcgattgtaa aggatttgtc cgtaggcaac ccctggtcga
     1141 tgaggatgta cccgaagagg gtctgcgggt cgggaacgaa ctgttgccgc atgtcggggg
     1201 ggaggctgtc aatgattgcc tggacttctg ccctcgtgcc agggattacc ccgccattgg
     1261 actgtgcaac cacgtctgca acggtggtcg ttgaactgac cgcgtcgata cggcggatct
     1321 gctcctcgag aatgaggagg tcggaaagga gttccgggtc cgtgatatcc cccgcatgga
     1381 tcaggagaat gtaggtgtcc tgcatgtagc ggttgctgta ggtgtcaaag acaaccccct
     1441 cgggcgcgct cttgtccatg tactcgtccg agattgtata gggcggaatt gcccccatga
     1501 acactactgc cacgatggcg agggcgacca tcgttacgag aactgctcgt ggaaaccggt
     1561 tcacgacgcc agcaacaact gcatatggat tgatccaacg catgcccggt tctgccctcc
     1621 tggatacttg acagtgtgtg gaagagctac attatttctt ctgtttcgat caagactgtc
     1681 catttcttcc ttttcccaag atatccttat ctcacccgtc cccccaggag taccatgcaa
     1741 aacgaccact tccctctcaa cacgccgttc aaaccctcgc cgtcactggt aacctggttc
     1801 atcatcgact ttctcttcct gatggtgttc ctggtcatcg tgatcccttt ttcgatgtac
     1861 ctttcatcag caatcgatac cgggatattt ttcggtatca tcggaggcat ggtggtgata
     1921 gtcatcctgt tcgccctctg gaccaaactc tactacgaga gcatgtggta cgaactccat
     1981 gaggacgaga tgcgctggaa acggggggtc atcttccgcc ggaccggaat cgtcccgtac
     2041 aatcggatca cgaacctgga catcaggcag ggaccagtga tgcggagact tggcatctcg
     2101 accatctcca tccagaccgc aggctactcg gcccaggccc aggctgagat acggatcgag
     2161 gccatcgtgc atgcagaaga actccgggag ctgatccgga gcatggtaag ggcaactata
     2221 ggaggggatg gcacgggaac aggagggacg gttccttcag tgaaaacaac cgaggttcag
     2281 atgctcgacg agttgaaggc tatccgggcg cttctcgaga agaagagctg acatccgggc
     2341 accctgggaa aacccggacc ctcatttttc caggatgaaa ctggttgtcg gatatctttg
     2401 ggagggagag aatgttgata aatcatcttg ggtatgttct ataaagcggg caagaaaata
     2461 gtgccagcag acctttcaga aaaaaaatcc tcaaaacagg agtactgcga atgaaaacca
     2521 gtggttgccg ggagtcttcc gcgataaaag ctctcatttc cgggttgatc gggatactct
     2581 ttttcctgat atttttagtc atactgggat acatcgccga tcatgtctcc tggccgctgc
     2641 tctcgggatt cgtcgatctc ttgtacgcca atgcagccct gatcatcatc ttctcggtgc
     2701 tcttcatgat cggggagatc ttcgctgcaa tcaggttccc cttcaatctc cctttcccgc
     2761 tcttcaatgc agtcgccagt gtgctgctcg tctcgttcat tctcgcgata atggccttca
     2821 tcgattcatt ttattcgctc ggcattggtt caaccctcca gtttgtgaaa ctgatcctgt
     2881 atcctctcac cttcattgtc gtcctgataa ccggataccg ctccatcttc tcgaagcctg
     2941 aagagaaatc cccggaggat agtgccccag tacctgacct gtcagataag gaagaagagc
     3001 ggaaaattag tccatcctgg gaggagatcg ggggagagtt caggcaggca gtctacgatc
     3061 tcatccgccg gatccgtgat gaactgaacc tgaaggaacg gcagtgaagg tataccggat
     3121 taaaagagag gtcttcagac gattcttcac tgtactattc tcagcagagc gatctattaa
     3181 atacacatac atacacagat acttccctat ggcaacccgg accatcagta tcagcgaaga
     3241 agcatacgag cgtttgaaac gtcttaaaaa gaatgaaaag atgagcttct ccgatgtaat
     3301 tctcgaaaat tttccaaaaa gacgcacact ttccgaagtg atgtcggaat tgggagattg
     3361 ttcagaactt gcagacagta tcgagatggc atcaagagag atcaggaaat cacggtttcg
     3421 tgaagtcagg gtatgagatg accgttgctg acacatcgtt cattatcgac ctgatgcgaa
     3481 aagatccagg tgcaacgatg atcttcgaac actacgaaca ggcaggaaga ggactggaca
     3541 ccacaggcat cactgctctt gagctgtata agggagcata tcagtcagtg aacagagaca
     3601 gtctggaaaa agtgaaaaat attctgtcgc tgttttccat ccttccgatc gatgaacagg
     3661 tgtatgaact gttcggcaaa ttgtcgacag atctgcgtat aaggggaacc ccgcttggag
     3721 cctttgatga agtaatcgct gcaatcgtac tctgcaatga tggagagatc atttcccggg
     3781 accggcattt cgggtcgatt cctggaataa cagtaatacc ctactaaaac gccgtcctaa
     3841 tcctggttct actccctttg ctttaaatct caggcgatcg gggatgctct ttctcttcac
     3901 tctccccaat ccggattttc ggtgagaaac gcctgctcct tcttctcgag ttcttccggc
     3961 gagaattttg ccggcatgca gatgcatcgt tcctcgacaa actcaccttt gacaaagcac
     4021 ccgaaggtcg tatcactgga gcatgccccg tattccttct tgtcatagtt gtgaaacacg
     4081 agttccgccc cttcacggtc ggcgatctcc tttgcttctt tccaggatcg tgctgccata
     4141 gttgatccag cttagcttca tcctgtcagg tacttaaatc ctcaggatcg tcccaggagc
     4201 aggacggtca tttttcatga atgctgatac cctcttctgt ctcacccgag cgaatcactt
     4261 cacatgactc ttctgctgat gaaaacaggg agaaactctg gtgatcacaa ggggttcctg
     4321 gtgattggta cggctgttcg ttcatcccta tggagggaaa ggcatagggt ttccctgtct
     4381 cgaaaagcag gttcaagaag tttttatgcc atcaaacgaa tctttcagag ttgaacaatg
     4441 aaaaaacaga aatttattga cctgaaagcc atcgcgtggg atgcgatgcg gaaatacggg
     4501 ttcgagccgg gattttcaaa ggcggtgatg cgggaggtcg aatcgatcga tgcgaagaac
     4561 gatcctgatg gaggaaccaa cgtgcgggat ctccgccccc tgctctggtc atccatcgac
     4621 aattatgatt ccatggacct tgaccagatc gagtactgcg aacgcggccc cggggatgag
     4681 atccatgtga tggttgccat tgcggatgtc gatcacttcg tcccgaagcg ttcccagacc
     4741 gaccaccgtg cagcccacaa cggcacctcg gtgtatacgg gggtcgagac gttccccatg
     4801 ctcccggacc ggctctcgaa ggggatcagt tccctcctcc ccgggaacga tcaccgggcg
     4861 atcgtgatcg aatacacggt gctcccggac ggggatgtac gcgaggaggg gatctcccgt
     4921 gctctcgtgt cgaacaaggc aaagctgatc tacgaggagg tcggcgaatg gcttgatggg
     4981 cagcggacga tccccccaag cgtgagtgcc gtccccggcc tcgaggagca gatccgcctc
     5041 caggccctgg caacgacccg gctgaagaaa tatcggatga agcagggggc tctcgaactc
     5101 gagacgatcg aggctgatgc cgttgcggag aatgggatcg tccgggatat cgttgtccag
     5161 aagatgaacg ccgccaggtt catcatcgag aacttcatga ttgcggcaaa cgggaccatg
     5221 gtggactttc tcggaaaagc cggcattccg atgatccagc gggtggtccg ggtgccccgg
     5281 tactgggacg agattgtgat gaccgccgcg gcctaccgcg agaagctccc ccgggagccc
     5341 gattcaaaag ccctctcgct cttcctcatg aaaaggaagg aggccgatcc cgagcggttc
     5401 ccggatctct cgctgaccat cgtcaaactc ctcgggcccg gggagtacat gacgctggag
     5461 ccaggagagc cccccaccgg ccacttcggg cttgccgtca ccgattacac ccacggtacc
     5521 gccccgaacc ggcgatatgt cgacctggtg aaccagcggc tcgtaaaatc ggtcatcgac
     5581 gggaaggaga gcccttacac cacccatgag ctctacgatc tctccacctg gctctccgac
     5641 cgggagaaag gctcaaagaa ggtggaacgg ttcatgcgaa aggctgctgc agcagtgctg
     5701 ctcaaggatc gcatcggcga ggagttcgaa gccctggtga caggggtcac cgagcatggg
     5761 atctacgccc ggatgatctc cccccctgcc gagggcagga tcctccaggg agagcagggg
     5821 ctccgggtcg gccagaaaat caccgtccgg ctgcttcgta ccgatcccta caacgggttt
     5881 atcgactttg cctgtgtcag gcgtggagag tcagggcagg ggatctatgt cgaacgtgct
     5941 ccccggagtc gtgcatcagc tccggttcca cggagatcgg gatatgggag aaagcaacgg
     6001 agcaggagat gagcccatgc ggatccaccc cttgcatttt ctctcctccc aacaaggttt
     6061 atcgtaattg agcaacagtg aaggagtggg aacgccatgt tcatcatgcc tgatttcatc
     6121 catacccccg aagaatggat cgtatggctg ctcggttcag aatttcatga gtctgcatca
     6181 gtcgatatgc tcgtccagag aacccgctac cgccgcgagg atatgctctc ttttatcggg
     6241 gtcctcgaac ggatgaaggc gatcagggtg aaccgcaacg cgagggacgc cgtgatcatc
     6301 gactcagttg ccctggtccc gaatggaaaa aggctgtatg gggagttgca gcagcgttcg
     6361 gggcagcagc cagggtgaag gaacttctgc agtcacttct catccggatc ctctggaaac
     6421 cggacaaaat agcagactcc ccattgcagc ttctctccgg ggctcaatcc gcgtaactgc
     6481 tgttctgcat gctcctcgca gacggtcgat gcacagcacc caaggatctg catcccgatc
     6541 gcctccctgc cgcacacatc acagatcttc ttttcgatgg acttctgatc cgggcacatg
     6601 ttctcttcct gtgatcatcg tcctcttccc tgatgaggct ctccattctc ttccggccgc
     6661 tccagggatc attcaccctt cttttccggg gaaggaattc tcacagatga acggtttacc
     6721 tggatatcca tcatctcctg cagtggttca gtctccaaga tccataatct gtacgggcga
     6781 atcatgtccc ctcccctcct ctccctgacg ctccccaatc ataacgaaca agacccgttc
     6841 cacaggatta tttaccgatc cgggaacaat cctcctttca tgcgaacgaa ccgccaccag
     6901 tatttccttg acctcgcgct ccgctgcgcc caccagggga cctgcctccg ccggaatttc
     6961 ggcgcgatca tcgtagacga gtacaatacc atcgtctcaa caggttacac cggggcacca
     7021 aggaagcagg tggactgtac cgaactgaac cggtgctggc ggaacgatca caacatcccc
     7081 tcggggtcaa actacgagaa gtgccggagc gtccatgccg agatgaacgc cctcctccag
     7141 gcaggaaaac tggcacgcgg ctgtaccctc taccttgcgg gatacgaggt agaaaccgaa
     7201 gcactcacgc agatctggcc ctgtttcctc tgctcgaaga tgatcgtgaa tgccggggtc
     7261 aggaatgtca tcatgcggac cggcgagacc acgtatgaag agatggaccc gatcgtgctc
     7321 taccagaagc ggagccgtga agcgctcggt gaatgacctc ctgtgcctga aggggaaggt
     7381 tgtcagggtt tcgagtgtgg tgcgttccag aatgagaatc ctggctctgg ttcagactcc
     7441 tgcatctcct gcagggaggg tccttctgtt ttccatcgcg gttttttgaa aaaaaagtta
     7501 ctgcccggtt gacgcgtcaa cccgctcggt cctggtcgtc ccggccgcat cacgggtggt
     7561 gatctgccag cggggtaccc acgcaaggaa gaacgagacg atctcgatcc cggtcttctt
     7621 tggtttcacc acgatctcct cccgttcttt taccgcattc tcccagaggg ccgtgatcgc
     7681 tgctgattcc ttctcaaact cgtccttcag ctcccgaagg tcatcttcaa gtgcacgggc
     7741 attctcctca gcttcccgtg cactcatccc cgccgaactg ctttgccggt acttgctcgc
     7801 agctgccgat ccgctccggg tgctcttccg tcccaggagg acaccgatga ccgtctcacc
     7861 ggccgagatc atcgcctccc gtttgcgggc atcagcatcc gcctgtttcc gatcggcagc
     7921 catctctgcc ttccggagcc ggtcttcgag cgtttcgatc ttcgcctcgt acttcttccg
     7981 gagggcatcc acttcggcat ccctccgttc ccgtgcagcc agcctgactc tcaggagaaa
     8041 ctcggtctcc ggttccccag ggtgtgagat gagttttaca gcggggcagg aggagaggtt
     8101 cagttcccgg gaatggacaa ggaaggtggt gagcgcctgc ttttcggtgg aaaatttctt
     8161 cagtgcccgg accatctcag cgggaacggt gtaacgtgcc tcggggggga ctgctgagga
     8221 gaggtcggtt cccagcccgg gatacggtac tgcactctcc catgtgacgc cacctgccgc
     8281 tgagaacggt gcgagaaggg caaaatcccg ctctgcatcc ttctccatct tcgtgctcgt
     8341 gtaatggatc ctgccactgg cgaggagtgc cggttcgtac agtaccgagg ctgcttcgac
     8401 tttcccgggt ctcgatcgga gtatggccgt ttctgcggtc tcacggggga ccctgacggg
     8461 aaggtaccgc tgcgggacct ccggggggat agcgggaagg agcggcgtag tatcggcaga
     8521 aagtggcaca gaggctgccg tggcaacggc atcctgctgg cgtactgtcg acatggctct
     8581 gtccggtgtc cttcctttca tcagggaacg gacctggtcc cggctgaggg gtccccggag
     8641 ataactcatc gcgaagcggg tctggaagat caccggggca ctctcatgaa cattgtgcag
     8701 gaggaacacc cggttatcca gcgatgaaag ggtatcagag agggtcgccc gggagaagct
     8761 gcccccctgg acagcaccct ccaggccatc gaggacccgt tcacggtcct gcacggtctg
     8821 gagcctgccg atgaaccagg ttccggtatt ggagagaccc ttgtaatcca ggtcgaccgg
     8881 gttctgggtc gtcaggacca caccgaggcc gaacgcccgt gcctgtttca gcagggtgag
     8941 catcggctcc ttgctcgggg gattagcaac cggggggaag aagccgaata tctcgtccat
     9001 gtagagaatg gcacggaggc tggtggtccc ggtctgggac cggacccatg caaggacctg
     9061 gtggaggagg agcgtgacaa agaacatccg ttcgtcctcg ttcaggtgtg caatgtagaa
     9121 gatcgaatgg cgtggtcgac cctccttcgt cgcgaggaat cccgggatat cgagcggctg
     9181 cccgttcatc caggactgga atgatggtgc ggcgatgata tggttcagcc tcatggcaag
     9241 ggcgaaccgt tcctttgccg ggaagaaggt ctcgatatcg aatgccccga tctgtttcag
     9301 agggggattc tggatcgcct ggatcagcgt taccaggtcc aggtcttttc cgttccgcca
     9361 gaactgttcg aagagcgagg agaggaggat gtgctcccgt gaactgagcg gatccgcggc
     9421 caccttgatc aggccaagga tggccgtcac ggtcccggag atcttctccc tgatgagttc
     9481 agcgtcagag tcccagctga ggggaggagc tgcaaaggat tgaatgacgg ataccgggac
     9541 gccggcttca ctccccggtg tatacactac aaggtccgca gcatcccgga gcatccggat
     9601 ccgtgctccg tcctggcccc acgaggcaag gccgctcttc catgactccg cctggcttgc
     9661 ggcaaactca tccggggact tccccttcct cctggcatcg tcagggttga tccaggggag
     9721 aaaatcggcg ggggcaaggt ccgggaacat gagcagccgg ttggtgatat cccccttggg
     9781 atcgatgatg atggcgggaa ccccgtcaat ggcagcctct tcgaggagat cgacgcagag
     9841 accggtctta ccactcccgg tcatcccgat acagatagca tgggtggtaa ggtcacggga
     9901 atcgtacatc acgagacggt cgaggagctt cttctctttg aggctgtact cttttccgag
     9961 gtaaaatgca cccagtctct caaattccat ggaaaacaag tactatgtgc tgacctggtg
    10021 taataaaagg attcgtccgc tcactccaga gagccagggc tgaaaattcc cggttgcggg
    10081 aacggtgggg ggcatcaacc gcggagcctc atcacagctg acgtatcaga gccccgtggt
    10141 tatctgcccg ggtcctgatg atcgttgcct cccggtcacc gaggagacca ttcacggcgg
    10201 cctcgagtcc ggccaggccc gaatcagtga tggcatagac agcagggcca aacgagctca
    10261 tccctgcaca ggcagcacct gcctcgcaga ggactgaggg gagctcccgg atggtgcggg
    10321 gctggcgttc ccgctcgatc cgcttgaatc cgatctcctg gatgcggttg accgcggcac
    10381 caaagagatc gagatcatgc tccaccacac cgggaaggag ccgcatcatc acctcatgac
    10441 agatctccct gacctcatcc accggaaccg ggcacgactc cctgaaaata tcggtctcca
    10501 tcgccccgct cacaggggtg tcatgttcgg gaatcacaag gaggatctgc cagtcgtccg
    10561 ggaagtcgtg ccgggtgatg acctctgccg gcctgatccc tccggacgct gaggagggcc
    10621 ggtactcctg cttctccttc ccctgaccga aactgtggcc gccgtcgacg atcagccccc
    10681 ctgactcgaa tgccgccgta ccgatccctg atgttccacc ccggccggtc atcacggcaa
    10741 ggtcccgtac cgggactggc atcccgtaca gttcgcagag cgccctggca acggagaggg
    10801 agagtgcagt ccctgaacca aggccgacat ggccgggcgg gaccgcccgg atgtggatcc
    10861 gggctccccc gcacatacca agcgaggaga gcaccatatg tgccgtatct cctgccctcc
    10921 tggcatacgg ggatgttcct ccgctgatga caaggtcggg cgattgttct gccgttatga
    10981 ccgtacgggg aagtgagagg gtgatcccga tcccgccatc tatccgccct agtgatccgt
    11041 tgaggtcgat gaggccaaga tggaggcgtg acggagcact gacaaggact ccggcaccca
    11101 tccggaacga gcaatcaggg aaaacctcct cgatcgagat gaacggtttt ccctgcctga
    11161 tgatacggta cttcctgaac agcaccggat tattgagagc gatgccaaat atccgggata
    11221 tgcgtgcatc ggagccccgg acacccacct gggatatctc cctccgggat tcgatggcat
    11281 gcttcttcag gatccttccg atcgggatat ccgcccgcat caggtcggat ctgaatccgg
    11341 gttccagccg ttcaagcggg gtatctgata ccgcgtagat gagaacctct ctcgtgcggc
    11401 tgttcatgag ctcgacgatt ctgtggttca cctgatcccc gggccggatc tccagttctt
    11461 ctgcagtctt tgtgtccgca ggacctacat tctgggagag tgtcctaatt gtcacctctt
    11521 ccccctcgat tgcttcaagg agggaggtta ccgatccatc ggtcgagagg aggatcttct
    11581 ggagcgtgga aagagttccg tgctcttttt caatccgttc cagcggtccc tccatacgca
    11641 ctcatccagt gactgaatga ccatttctct gcagggagta taagttgatt gtgccagagg
    11701 gagatcagga gggacctgca ggggaacggt tccggtatcc tgtttgaatg ccgggcatcc
    11761 gggaaaaagg gtttaaaaca tgaggaagat aaggaaggct aacggcttgt cctggttttt
    11821 aagagaaccg ctgtatcgcc gaaacaacgg tattgaccac gaatctgtct gactgaccgg
    11881 acggatacca ccccaaggag gtgacccatg catccgaagt acatccatac catcgatgag
    11941 ctcgacaacc ttgtcgggct tcctggaaaa gagaagagag agatggagca ggtctgtcaa
    12001 acgttcccgt tcagggctaa tgactactac ctgtctctca tcgattggaa taatgcccat
    12061 gatccgctca ggaagattgt catacccgat ccgcgggagc tgaaaggggg cggctccctt
    12121 gatccttcgc acgagaaaga ttacacgaaa cttcccggcc tgcagcacaa gtaccagccg
    12181 acggggctgt tgttgctctc ggatgtatgc ggggggatct gcaggttctg tttcaggaaa
    12241 aggctgtttg tcaactgcga acgcgagacg gttaaagacg tatcccaagg attagaatac
    12301 attcggaccc atcctgagat caccaatgtc ctgctcaccg ggggtgatcc actcatcctc
    12361 gagacatcat accttgagcg gatcctgaaa gaattacggg agatccccca tgtgaacatc
    12421 atccgtatcg ggagcaaggt tcccgcctat aaccccttcc ggatcctctc agatccccac
    12481 ctgtccgagg tactctctcg atataccact gtcgagaaga ggatatacct gatggcccat
    12541 ttcaaccacc cgaacgagct taccacacag tcattgcagg cgctcgagca gctgcaccgt
    12601 gctggggtga tcgtggtcaa ccagacgcct gtcttgaacg aggtgaacag ttccccggag
    12661 acgttcacca cactcttccg caagctctca ttttcagggg tgacgccgta ctatgtgttc
    12721 caatgccggc cctcactggg gaacaggttg ttccaggtac cggttgaaca gtcataccat
    12781 gtgatccagc aggcctggca ggcgtgctcc gggctcgcaa agagggcacg gttcgtgatg
    12841 tcccatgcaa cgggaaagat cgaagttgtc ggcaggcagg cagggtgtgt cttcatgcgg
    12901 taccaccagg ctgcagaaaa agccctgatt gggaaattca tggtgatgaa gagcaatccc
    12961 agtgctgtct ggttcgatga ttacagggaa atccccttgg aaaaacctgt cccaagaaaa
    13021 gtctggttat tctgacccga acggtaacag gacaggggaa cgcgatcccc tttccctttc
    13081 tgcttcactc tctgtgaccc ttttctcccc ccgtcacacg agaaacgaga tgatcatgag
    13141 gaacaatatg agaatggcga ggaaccatac cacccggatg ataacctgcc ttgattgtat
    13201 ctgggcgaag atatcccgtg ggccgatatg gagaaagatc actccgatga tacttccggc
    13261 aatcagaccg atcacattct gcagcgtcag gacaatcgct tttaccgccc ctgcgggaaa
    13321 aagggccagt gagatgccgg tgacgactgc aggtggaagg agtgctgcgg caattgccac
    13381 tcccgcgatc ctttccggaa ttccctctga aagggcgatc atcgttgcaa agccaaggag
    13441 gacggccatc agaatgaata ccgcattggg gtccgtccgc gataggatct ccggggtcag
    13501 ggtgagatcg atgacgaagg agagggcaaa tgaagccacg gcagcgataa agaccagcat
    13561 cagcaccatc agtcccagga tctccacgca gcggacggtc gttttcacat caccgacggc
    13621 aacgtagacc gccagggcat agatcggacc gagcaggggg gatatcagca tcgcaccaat
    13681 gataataccg gcattgttca ggaacagccc gatcatcgcc acaatcccgg cgatggccgc
    13741 gaggatgaat ttctcctgat caaaccgggt attggactcg gtcgatgcga ggatcttctc
    13801 tatgggggtt ttctcatccc gcttctgctt cttggtgtat cccagcttct ccttgagtct
    13861 ctcgacaaag ggggagatga caaaatccgg ggtggagacc tcaataagaa tactccggtc
    13921 atcttctttt gccaggctct cataatcggg aatgaatccc gtccagagtg gcctcgtggg
    13981 atacgtatct ttgaccgatc cgttggtctt gtggatcaga tcatcaagca tctcgtccgg
    14041 cacatagagg atgaacctgg tcgatgcttc gccctcatgg attgaaaaga tggtatccct
    14101 gaatttatca tgtattttct ctgctttgtc attggggacc tcgatgacca ctcgtttcat
    14161 ggaaggatac cagctattgc atttttcggg aaaagcacaa taaaggtatt ggccccggtc
    14221 gttcgtatct cctggttgaa gtgtctggca attggtaccg gctccgacga ttcagggttc
    14281 gaggtctgcc taccctatcg gaaacacatc cctctcctga gggtgccttc gttcagacca
    14341 attttttgga gaacatcatc agttcatttt ttcaaccaga acggtggtga tggggagatc
    14401 ctcatctcat tcatcagaaa agaaggaaga tcaggatcac agccacagca atgaagatca
    14461 cactcatgat gccccctgct ttcaggtaat ctctcgtggt ataacccccg ggacccatca
    14521 ggagcgcatt cacctggtgg gtggggagga taaatgaatt ggaagcgcac accccgacaa
    14581 ggagtgcaag ggcccggggg tcgatccctg tcccggcccc ggtaaccatc gcaatcggga
    14641 cgagcagtac ggttgcggca acatttgaga tgacgagcga gaggagagta gccacgatgg
    14701 cgattgcaac caggacaaca aggggatggg ttccggaaag atatgcggag agcgaactgg
    14761 cgagcaacgc agcagccccg ctcttttcaa tcgcgatccc gagcggtatc aggccggcga
    14821 tcaggatgac cgtcttccat tcgatggcat gatatgcctc gtcgatggta aggacatggg
    14881 agagcatcat cgcggctgta ccggtgagaa atgcgaggga gagcgggacg ccggtcaacg
    14941 tgagggcgat ggagaacgcg aagatcagga ccgcgagggg ccccttgcgg taccgtaccc
    15001 cttccccctc ggggggtgag atgagcagga gatcccggtc ccttgccagg tgtctcaacg
    15061 tatcccatga tccgaaagcg acgatgagat caccggcggc cagtgataag ccggagagat
    15121 caccacgggt ctccgtctct cctctctgaa ggaggatcgg ctcgattgaa aacgtctggc
    15181 ggaacaggat ctcctgcggt gtttttccga taatcgatgc ccttggtctg acgatcagct
    15241 cggcgaaccc gaatccttct ccattgagga tctccccaag gatgctccga tcgcctgatt
    15301 gcacacagcc tgaatcttca aggaaccttt taaagtcctc atcggatcca aggaaggcta
    15361 gttcctgccc gggctcaaaa cgggtggatc gggggggtgc gacggtgata tcctgtcccg
    15421 ctctgatcgc aag
//
LOCUS       DAOOCO010000020        15107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3565_length_15107_cov_5.329657, whole genome shotgun sequence.
ACCESSION   DAOOCO010000020 DAOOCO010000000
VERSION     DAOOCO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_15107_cov_5.329657"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="rpsE"
                     /locus_tag="PK154_02180"
     CDS             <1..378
                     /gene="rpsE"
                     /locus_tag="PK154_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPW09902.1"
                     /translation="NGYIGFGQGKDSQVGDAIKKAIVDAKMNVIKVRRGCGSWECGCD
                     TTHSIPMKVEGAAGSVRVMLKPAPQGIGLVTGEISRKVLQLAGIKDAWTFSKGQTRTT
                     INFAKATFNALKATNMTRRGGDE"
     gene            378..839
                     /locus_tag="PK154_02185"
     CDS             378..839
                     /locus_tag="PK154_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPW09903.1"
                     /translation="MYAIVQVRGVVNTRREIKDTLKMLRLHHINHCVLVPDTPEYLGM
                     IRKVKDFVAYGEVDAVTIETILRTRGRVIGDTPLTDEYVKSNSLYSGITEFAAALASG
                     ETRLADMPGLKPVLRLHPPRKGYRTIKRTAQQGGALGYYGQEINALLHRMR"
     gene            846..1271
                     /locus_tag="PK154_02190"
     CDS             846..1271
                     /locus_tag="PK154_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HPW09904.1"
                     /translation="MPVNKRSKYRGSRTCGGGTHKNRRGAGNRGGRGRAGHRDHRFSH
                     YLLYGEIHNGKDGFYNQTARIMRVISVGEIDQMAPSLLAGGKATREGDTITLHADQIG
                     IDKILGSGKVTLKMNITARAFSAQAKIKIEAMGGQALVI"
     gene            1292..2731
                     /gene="secY"
                     /locus_tag="PK154_02195"
     CDS             1292..2731
                     /gene="secY"
                     /locus_tag="PK154_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPW09905.1"
                     /translation="MGTLLDRMEPLLAAMPAVKSPEGHVHFKNKLLWTAGILILYFVM
                     TNIPLWGLDPNSMDLFQYYRALLAGASGSLIQLGIGPIVTASIVLQLLKGADILHIDT
                     AEVRGQVLYMGLQKLLIFVMIVIEALPNLVGGFLLPDPVVADMFFGGSLTAVSFIIFI
                     QICIGGVLIMFMDEVVTKWGIGSGVGLFIIAGISQAIINGLISWIPVTDQYAVGFFPR
                     LVQVIMDGADFLQYMGPELLAFITTIAIFLIVTYVESTRIEIPLAHAQVRGARARFPV
                     KLIYASVLPMILVRVLQANIQMIGLFLYNIGITIFGTFSGAQAVSGLMYYLAPINGPS
                     DWMWWNPAYGITNAPWQILIRLGIDVTFMVVGGAIFALFWIKTAGLDSKDVARQIQLS
                     GMSIPGYRRNPQVLEKYLDRYIPRVTVIGGVFIGVLSVFANLFGVIGAVSGTGLLLTV
                     SITYRLYEEIASQQIMEMYPFMRTFFGKE"
     gene            2734..3306
                     /locus_tag="PK154_02200"
     CDS             2734..3306
                     /locus_tag="PK154_02200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPW09906.1"
                     /translation="MKGRSVIVTGVPGVGKTTVVTGALKILDQEGIIYKTLNFGTYMF
                     DVAQKEGIAKDRDEMRRLDKDVQKRLQKRAAQEMAKEEGNVLIDTHASVKTPKGYLAG
                     LPEWVLRELMPDTIILVETDEDQILLRRLTDETRTRDLEGSYSIGEHQQFNRSIAAAY
                     SMMTGCTIKYVTNADHLLEKAVEDMAAILR"
     gene            3314..3913
                     /locus_tag="PK154_02205"
     CDS             3314..3913
                     /locus_tag="PK154_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF106 domain-containing protein"
                     /protein_id="HPW09907.1"
                     /translation="MAKRSYGGILALVVAMVLIFSYSIPWVRDTVGGAIDVLFGPLAE
                     SLPFYIFIVVLSTLTAIYSSLIQKYTIDYEKMQDTQERMKAFQKEYREAQLSGDDKRI
                     KKLDSKRDKMMKDQLEMSQQQFKPMAYILIVSVPIFFWLLYRLNNFVDNIGITMPFFG
                     PHLLSDFILGPIPAWILWYMICSLTISQVVRKALNIGGI"
     gene            3913..4449
                     /locus_tag="PK154_02210"
     CDS             3913..4449
                     /locus_tag="PK154_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW09908.1"
                     /translation="MRITVSGLPGSGTTSLARYIAGALHVELISAGEVFRQMAREHGM
                     ELAEFGRLAESDPSFDHLIDERQKEIALANDDIIVEGRLSGWFVPEAELKIWLYASLE
                     CRLCRIQTRDFIGNSDDAAQLTRERESSEAIRYRMYYGIDIADLSPYHLVLNSERFAV
                     EELGELVCSAIDVVRKRN"
     gene            4652..5074
                     /locus_tag="PK154_02215"
     CDS             4652..5074
                     /locus_tag="PK154_02215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW09909.1"
                     /translation="MESGISVQVVKEWDSAVLVDLYRAGGWWMQDHDPAHLPALVRSS
                     FAFAIAVEEATGRSVGMGRIISDGVADGYIQDLVVIPEFRGKGVGRNILRTLLAFCYE
                     RNITWIGLIAEPGTEEFYTSIGFSRMKDHIPMRYRGRL"
     gene            5081..5989
                     /locus_tag="PK154_02220"
     CDS             5081..5989
                     /locus_tag="PK154_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPW09910.1"
                     /translation="MLSVSDFSPVRLEDRPLFVSHYQRFPQVHSDNTFSNMICWNSYA
                     HYRFAQLKENILISSTIEGKTSYRPPIGRRDPELLADLFELCIHSGNEIPLSLVDTTQ
                     RAWILSEYPDLPLYPDRDYFEYVYQTEDLATLPGKDYLSIRRHLNRFRNNCKPVVEPL
                     ETQNLDEGADFLVEWCEWKNCDSEPFLGHEKEAMLCALSHMAELGMRGLLIRVVGKVG
                     AISLFEPLNEDTLVVHFEKGLPDCEGIYKAINAETAFAAKEKYRYINRESDMGVPGLR
                     EAKMRYHPHHMVEVWYATLDDMERIL"
     gene            complement(6091..6642)
                     /locus_tag="PK154_02225"
     CDS             complement(6091..6642)
                     /locus_tag="PK154_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPW09911.1"
                     /translation="MFTSMTQGQSSSEINRQALIAVAVAGFLMYLFLFFNFFIFDTLG
                     ITLASFQVAGGILLFLLGLQMALGIDIGKTKEHTPTAAGVIIGTPLLCGPGTITTVLL
                     LSEENGMIITALAIALVLAATWIVLRFSFQIQKLLGNTVTEILAKILGMLVAAIAVSI
                     AADGIIGLLQDYGTIYSFSLNVK"
     gene            6536..6856
                     /locus_tag="PK154_02230"
     CDS             6536..6856
                     /locus_tag="PK154_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09912.1"
                     /translation="MKKRNRYIKNPATATAIRACRLISDELCPWVIEVKIGTESSGSR
                     MTKRAENEYISAPGRSAISQQYTLSFGIKGLVPGGIFQEKEYKNISLLEERALLSRLN
                     VLRV"
     gene            complement(7030..8388)
                     /gene="ftsA"
                     /locus_tag="PK154_02235"
     CDS             complement(7030..8388)
                     /gene="ftsA"
                     /locus_tag="PK154_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F390 synthetase"
                     /protein_id="HPW09913.1"
                     /translation="MVESGFFREEVECLPRDDLNALIDERVRYTIRYAAEHSPFYHAW
                     FRKHRIDPASVETHQDLIKLPLISGSTIRENQPPVTKEFMFRSVEWSDVFTIHETSGT
                     SGIPKSFFLTWEDWLRYAEKYARSFVSQGFSHGDRIVICASYGMNVGANTMTLAARDI
                     HATIIPIGKCNFPARIINSYHPTGIVGSVFKLLRLAERMRAEGIKPEESSIRALIVGG
                     ESFADESRAYVSEVWDREVFNTYGSTEGTMCGECHVRQGLHVPEDLVHLDVYDPTLGD
                     FVEDGECGRAVLTTLLPVGGKTGTLLINYDTEDTTVVTTRDTCPCGRTHMKIMNPQRE
                     AETVWILGTPVNRVDIERGVFQRENMEYLSGEYEAFVYESEETADTVLRVTFETKGVI
                     TGPEKELVMANFKRSFYRYKTALSRLSSDTFSLVFSFTEQGGLELSRIKGRPKRLVDR
                     RS"
     gene            complement(8475..9140)
                     /locus_tag="PK154_02240"
     CDS             complement(8475..9140)
                     /locus_tag="PK154_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW09914.1"
                     /translation="MTPSGIIALILILTTLLSGCVFNPELLPERDDANVTALTSVEIR
                     EYQGERLSSITDFRENSLEGPQYINQSAYLLEINGLVSHPLLLPYEKVITEYPPFQKK
                     VTLFCVEGWEVTILWEGVRVKDLLNEAGVNPAANTVIFYAADGYSTSLPLDYIEDRDI
                     LLAYSMNGVTLPAERGFPFQLVAEDRWGYKWIKWVTRIEVSDDPSYRGYWEKRGFSQS
                     GER"
     gene            9285..9668
                     /locus_tag="PK154_02245"
     CDS             9285..9668
                     /locus_tag="PK154_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW09915.1"
                     /translation="MPPVERYKCRVCGYIYSPLRGEPHNGIPAGTKFDDLPESYICPI
                     CGMQGKGKIGKWGFEEWVPTRWVCSVCGYVYDQKRGEPHRGIKVGTAFEDLPEDYVCP
                     VCALDPKIKVQFGKVFKNGFEPLEL"
     gene            complement(9783..10307)
                     /locus_tag="PK154_02250"
     CDS             complement(9783..10307)
                     /locus_tag="PK154_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW09916.1"
                     /translation="MHGTGRDPVREAMMRHEERQEQNLSILIKQLSDPNSTLRARSAE
                     SLGNMGDERAVEPLILLLGDQVPDVVWVAIRALGTLRDPRAFEPLIRCIDSPDRWTRQ
                     GAAWALGELGDPKAGPVIIPLLTDKKKGVRQSAAEALGKIGEIRYREYLEPLLDDEEL
                     EVRHTAREALKKLK"
     gene            10448..11293
                     /locus_tag="PK154_02255"
                     /pseudo
     CDS             10448..11293
                     /locus_tag="PK154_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            11321..11764
                     /locus_tag="PK154_02260"
     CDS             11321..11764
                     /locus_tag="PK154_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW09917.1"
                     /translation="MTLDVSVRELEKDEFPLAEKVWAHYHQQKADSRTDRIFGVFVEG
                     MLVAIARCRRHPDGLEVDGVFTLDDFRGRGYARDVMQPLVSACGNEILYMHSTLGLVE
                     FYRQFGFEKIPEQNLPPTIKARFDFAMGEMQGSNVQPMMRRATGQ"
     gene            complement(11845..12882)
                     /gene="mtrH"
                     /locus_tag="PK154_02265"
     CDS             complement(11845..12882)
                     /gene="mtrH"
                     /locus_tag="PK154_02265"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HPW09918.1"
                     /translation="MFRFEKEQQVWDFNGTKIGGQPGEYPTVLACSIFYNKHEIVLDD
                     KTGKIDKAAAEALWNRCQELSDLVGIPHFIQILAEYPAAFESYIDWFCSLDSKTAFLM
                     DSSVPAALAHACKYVTDTGVANRAIYNSINGSIMPENIEALKNSDVNSAIVLAFNPAD
                     PSVAGREKVLVEGGVAGQTKGMIQIAEECGITRPILDTAATPLGLGSGSAYREILACK
                     AIHGYPTGGAYHNMTVAWTWLKRWKGSKKNPSALAESLKGKDVYLKQLLHHYKGGLDG
                     VIQAAWSAPDIGCNLVASTLGADLIMYGPIENVEPMITAQAYMDIVTLEAVRPLGIDV
                     KAETHPIFKLI"
     gene            complement(12900..13637)
                     /gene="mtrA"
                     /locus_tag="PK154_02270"
     CDS             complement(12900..13637)
                     /gene="mtrA"
                     /locus_tag="PK154_02270"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HPW09919.1"
                     /translation="MADKKSPASGWPIVQGDFHAGDANSPIAVMTCGSHLDEKGICDK
                     GAAICGSCKTENLGLEKVIANIISNPNIRFLLLCGTEVKGHLAGQTMAALHKGGVKDG
                     RVVGAEGAIPFIENLADDAIKRFQEQCELVNIMESEDLGTIGAKINELKGRDPGAFAA
                     DPMVVEVKEAAGGVETAAAGVNPQFLEIEKRLDKIEKQIEFADAEIAQRVGRKIGRDI
                     GILYGLVAGLTVFVLLLVLLPKLNVLL"
     gene            complement(13648..13854)
                     /locus_tag="PK154_02275"
     CDS             complement(13648..13854)
                     /locus_tag="PK154_02275"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit F"
                     /protein_id="HPW09920.1"
                     /translation="MAEEEAKVAAGPIRMVAIDRMVENMRYKSQLIARSNKFDSAMSE
                     MGIIGFAIGLVVSLVLIVVPAMFM"
     gene            complement(13861..14583)
                     /gene="mtrA"
                     /locus_tag="PK154_02280"
     CDS             complement(13861..14583)
                     /gene="mtrA"
                     /locus_tag="PK154_02280"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HPW09921.1"
                     /translation="MADKKSPASGWPIVQGDFHAGDANSPVAVMTCGSHLDEKGICDK
                     GAAICGSCKTENLGLEKVIANIISNPNIRFLLLCGTEVKGHLAGQTMAALHKGGVKDG
                     RVVGAEGAIPFIENLADDAIKRFQEQCELVNIMESEDLGTIGAKINELKGRDPGAFAA
                     DPMVVEVKEAAGGAEEAGGVVQPMSGELALIHARMKIIERMVTDIGYRDKFASGVYSG
                     KIEGIMIGLIVSFALLGFILMG"
     gene            complement(14594..14878)
                     /gene="mtrB"
                     /locus_tag="PK154_02285"
     CDS             complement(14594..14878)
                     /gene="mtrB"
                     /locus_tag="PK154_02285"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HPW09922.1"
                     /translation="MMYVQVLPEYGLVMDPMIGVVTTAGESLAPVIDKVAELETVAND
                     IVNMLSGTGNFAASFPGRENTLPMAGGMTAFWYGLAVGLFIAGIYVFHLI"
     gene            complement(14875..>15107)
                     /locus_tag="PK154_02290"
     CDS             complement(14875..>15107)
                     /locus_tag="PK154_02290"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HPW09923.1"
                     /translation="NWTQDRMLMLAAECGFLSMITAAIMSMPLISMSAALVSLFVAII
                     GWYYTYAKYIELSKRDAAAWLESKPIPDVEGH"
BASE COUNT         3581 a         3853 c         4002 g         3671 t
ORIGIN      
        1 aacggctaca tcggatttgg ccaggggaaa gattcccagg ttggcgatgc aatcaagaag
       61 gccattgtcg atgcaaagat gaacgtgatc aaggtcaggc gcggatgtgg atcatgggaa
      121 tgcggttgcg ataccaccca ttccatcccg atgaaggtag agggtgcagc ggggagcgtg
      181 cgggtcatgc tcaaacctgc cccccagggg atcggcctcg tcacgggtga gataagcaga
      241 aaggtgctcc agctggcagg gatcaaggac gcctggacgt tctcgaaagg gcagaccagg
      301 acgaccatca attttgccaa ggccaccttc aacgcgctca aggcgacaaa tatgacccgg
      361 cgcggaggtg atgagtgatg tacgccatag tacaggtccg cggggtggtg aatacccgcc
      421 gtgagatcaa ggatacgctc aagatgctcc ggcttcacca cattaatcac tgcgtccttg
      481 tcccggatac gcctgaatac ctcggcatga tccggaaggt caaggacttc gtcgcttatg
      541 gtgaagttga tgccgttacc attgaaacga tccttcgcac acggggacgt gttatcggag
      601 ataccccatt gaccgatgag tatgtgaagt caaattcact atattcaggg attaccgaat
      661 ttgcagcagc tctggcttcg ggtgagacac gacttgctga catgcccgga ttgaagcccg
      721 tcctccgtct gcacccgccc cggaaagggt acaggacgat caaacgaact gcccagcagg
      781 ggggggcact cggctattat gggcaggaga ttaatgcgct cctccacagg atgaggtgaa
      841 aaaagatgcc ggtaaacaaa cgttcgaaat accgcggatc acgaacatgc ggtgggggta
      901 cccataagaa caggcgtgga gctggtaaca ggggcggaag aggacgggca ggtcaccgtg
      961 atcatcggtt ctcccactac ctcctgtatg gtgaaatcca taacggaaag gatggattct
     1021 ataaccagac cgccaggatc atgcgggtga tctcggttgg tgagatcgat cagatggccc
     1081 cttcccttct tgcaggggga aaagccaccc gggaggggga tacgatcacc ctccatgcag
     1141 atcaaatcgg tatcgataag atacttggaa gcggaaaagt cactttaaag atgaacatca
     1201 cggcaagagc attctccgct caggcaaaga tcaagatcga ggcaatgggt ggtcaggccc
     1261 tggtcatctg accaattttt agggtgtaag aatgggaaca ctgttggatc gcatggaacc
     1321 gctgcttgct gcaatgccgg cagtaaaaag tcccgaaggc cacgtccatt tcaaaaacaa
     1381 gctgttatgg accgctggca tactcatcct gtatttcgtt atgaccaata tccccctctg
     1441 gggacttgat ccaaattcca tggatctctt ccaatattac cgggctctgc tcgctggtgc
     1501 gagtggttct ctgatccagc tgggtattgg tccgattgtc acggcatcca ttgtcctgca
     1561 gctcctgaag ggtgcagaca tccttcatat cgataccgcc gaggttcgtg ggcaggtcct
     1621 ctacatgggc ctccagaagc tcctgatctt tgtgatgatt gtgatcgagg cacttccgaa
     1681 cctggtagga ggatttctcc tgccggatcc tgtcgtagca gatatgttct ttggcgggag
     1741 tctcaccgca gtctcgttca tcatcttcat ccagatatgc atcggcggag tgctgatcat
     1801 gttcatggat gaagtggtca ccaagtgggg tatcggatca ggggtcggtc tcttcatcat
     1861 cgccggtata tcacaggcca tcatcaatgg tcttatcagc tggataccgg taaccgatca
     1921 atatgcagtg ggattcttcc cgcggctcgt acaggtgatt atggacgggg cagatttcct
     1981 gcaatacatg ggtcctgagc tgctggcgtt catcaccacg attgccatct tcctgatagt
     2041 cacctacgtg gaatccaccc ggatcgagat cccgcttgcc catgcacagg tgaggggggc
     2101 aagagcacgc ttcccggtaa agctgatcta tgccagtgta ttacccatga ttctcgtgag
     2161 agtgctgcag gcaaacatcc agatgatagg gctgttcctc tacaatatcg gaatcacgat
     2221 atttggtacc ttctctggcg cgcaggctgt cagtggtctg atgtattatc tcgcaccgat
     2281 caacggaccg agtgattgga tgtggtggaa tccagcatac ggaattacca atgcaccatg
     2341 gcagatcctc atacgtctcg gtattgacgt cacgttcatg gtcgttggag gagcaatttt
     2401 cgccctgttc tggataaaaa ctgcaggtct cgattcgaag gatgtggccc ggcagattca
     2461 gctgtcaggg atgtcgatcc ctggctaccg gagaaatccc caggttcttg aaaaatatct
     2521 cgaccgctac attccacggg tgacagtaat cggaggggta tttatcggag tgctttcggt
     2581 ctttgcaaac ctctttgggg tgatcggggc agtgagtgga acggggcttc tcctgacagt
     2641 gagtatcacc taccggctct acgaggagat tgccagccag cagatcatgg agatgtatcc
     2701 gttcatgagg acattctttg gaaaggagta agtatgaaag ggaggagcgt aatcgttacg
     2761 ggtgttccag gggttggcaa gaccaccgta gttacggggg cgttaaagat cctggatcaa
     2821 gagggcatca tctacaagac cctgaacttt gggacgtaca tgttcgatgt cgcacagaag
     2881 gaaggcatcg cgaaagaccg ggatgagatg cgcaggcttg acaaggatgt tcagaagcga
     2941 ctccagaaac gtgccgccca ggagatggca aaagaggagg gaaatgtcct gatcgatacg
     3001 catgcatcgg tgaaaactcc gaaaggctat cttgcagggc tccctgaatg ggtccttcgt
     3061 gagctgatgc cggataccat catcctcgtt gagacggacg aggatcaaat cctcctccgg
     3121 aggctgaccg atgagacaag aacccgtgac ctcgaaggat cctacagtat cggcgagcat
     3181 cagcagttca atcgatccat tgctgcagca tattcgatga tgaccggctg taccatcaaa
     3241 tacgtcacga atgcggatca cctccttgaa aaagcagtcg aagacatggc agcgatcctg
     3301 cggtgaatgt gaaatggcaa agaggagtta cggagggata cttgcccttg tcgtggcgat
     3361 ggtgctgata ttctcctaca gtatcccctg ggtccgagat actgttggcg gagccattga
     3421 tgtactattc ggccctcttg cagagagtct cccgttttat atcttcatcg tggtcctttc
     3481 tacactgact gcaatctact cctctctcat ccagaagtat actattgatt acgaaaagat
     3541 gcaggatacc caggagcgga tgaaagcgtt tcagaaggaa taccgggaag cgcagctctc
     3601 tggtgacgac aaaaggatca agaagctcga ttcgaaacgg gacaagatga tgaaggatca
     3661 gctggagatg tctcagcagc agttcaaacc gatggcttat atcctcattg tttcagttcc
     3721 catattcttc tggcttcttt atcgtctgaa taattttgtg gataatatcg ggatcaccat
     3781 gccgtttttt ggtcctcatc tcctctctga ttttatcctt ggccccatcc ctgcatggat
     3841 cctctggtat atgatctgct ccctcaccat cagccaggtg gtaagaaaag ccctgaatat
     3901 cgggggcatc tgatgcggat cacagtgagt ggtcttcccg ggagcgggac cacatcactg
     3961 gcgagatata ttgcaggcgc ccttcatgtt gagctgatct ctgcgggtga ggtgttcagg
     4021 cagatggcgc gggaacatgg gatggaactg gctgaattcg gacggcttgc agagtcagat
     4081 ccttcatttg accatcttat cgatgaacgg cagaaggaga ttgcactcgc gaacgatgat
     4141 attattgtcg aaggccgcct ctcaggatgg ttcgttccgg aagcagaact gaagatctgg
     4201 ctctatgctt cgcttgaatg caggttatgc cgtatccaga cccgggattt cattggaaat
     4261 tctgacgatg cagcacaatt gacgcgtgaa cgggaatcat ctgaagcgat ccggtacagg
     4321 atgtattacg gcattgacat agctgacctg tctccttatc acctcgtgct gaactcagaa
     4381 cggttcgcag tagaagagct cggggaactg gtctgctcag caattgatgt ggtaagaaaa
     4441 cggaactgac gttttggcct caaaatccca tccgcatttt tcatcaggga aagccattaa
     4501 acccagcgat atcctatcgg tttcataccc ttttgggtgt atctcttata cagaactatc
     4561 atggtgcagc gatatgacca tcgaacaaga cctgattgca gagcacagtg tacaatgaac
     4621 aggtgttaag ccagtgccgg ggataaagaa gatggaatct ggaatttccg tgcaggtcgt
     4681 aaaagagtgg gatagtgctg tgctggtgga tctctaccga gccggaggat ggtggatgca
     4741 ggaccatgat cctgcccatc tgcctgccct ggtcaggtct agttttgcct ttgctatcgc
     4801 cgtggaggag gcaactggaa gatcggtagg gatgggcagg atcatatcag atggcgttgc
     4861 tgatgggtat attcaggacc ttgtggtcat tcccgaattt cgcggaaaag gcgtaggccg
     4921 gaacattctc cggactctgc ttgccttctg ttatgaaagg aacataacat ggataggtct
     4981 gattgctgaa ccgggtacgg aagaattcta tacatcaatc ggtttctcac ggatgaaaga
     5041 tcacataccg atgaggtatc gtgggaggct gtgagtaccg atgctgtcag tatctgattt
     5101 ctccccggtg aggctggagg accggccgct ttttgtctcc cactatcaaa ggtttcctca
     5161 ggtacattct gacaacactt tctccaacat gatatgctgg aactcgtatg ctcactaccg
     5221 gtttgcacaa ctgaaagaaa acatccttat ttcaagcacc attgaaggga aaacctcata
     5281 tcgtccgcct atcgggcgga gggatccgga acttcttgct gatctttttg aactgtgcat
     5341 ccatagcggg aatgagatcc ccctctcact ggtcgataca acgcaaaggg catggatcct
     5401 ttcagaatat cctgatttgc ccctgtatcc cgatcgtgat tacttcgaat atgtctacca
     5461 gaccgaggat cttgccactc tgccaggaaa ggattacctc tcgatacggc gccacctgaa
     5521 ccggttccgg aacaactgta aacctgttgt tgagccgctt gagacccaga atctcgatga
     5581 aggagccgac tttcttgtcg aatggtgcga gtggaaaaat tgtgacagcg aacctttttt
     5641 agggcacgag aaagaggcaa tgctctgtgc gctttctcat atggcagaac ttggcatgag
     5701 agggctcttg atcagggttg tgggcaaagt cggcgcaatc tctctctttg aacccctgaa
     5761 cgaagacacc ctggtggtcc attttgaaaa aggactgcct gactgtgagg gaatttacaa
     5821 ggccatcaat gcagaaactg cattcgccgc aaaagaaaag taccgctaca ttaaccgaga
     5881 gagtgacatg ggtgtccccg gactccgtga ggcaaagatg cggtatcacc cccatcacat
     5941 ggtcgaggtc tggtatgcga ccctggatga tatggaacgg attctttaaa cccgtctctt
     6001 ttattttagt acgggtagtg acctttatac ccgggtaaga tcagttcagt cattgccagt
     6061 ctctctcatc ccaaatagga atcattgatt ttactttaca ttcagtgaga atgaataaat
     6121 cgtcccataa tcctggagca gaccgatgat gccatctgca gcgatactta ctgcaatggc
     6181 agcgacaagc atccccaata tctttgcaag gatttcagtc acggtgtttc caaggagttt
     6241 ctgtatctgg aaggaaaacc gaagaacaat ccaggtggca gcaaggacga gcgcaatggc
     6301 aagtgccgtg atgatcatgc cgttttcttc cgagagaagg agaactgtcg tgattgtacc
     6361 aggaccgcag aggagtggcg ttccaatgat cacccctgcg gctgtggggg tgtgctcctt
     6421 cgtcttcccg atatcaatac cgagggccat ctggagacca aggagaaaga gcaggatgcc
     6481 tccggcaacc tgaaagcttg cgagcgtgat ccccagggta tcaaagataa aaaagttgaa
     6541 gaaaaggaac aggtacatca aaaatcctgc aaccgcaacg gcgatcaggg cctgccggtt
     6601 gatttcagat gaactctgcc cctgggtcat tgaggtgaaa atcgggaccg agagcagtgg
     6661 gtcgaggatg acaaagaggg ctgagaatga atatataagt gcccctggga ggtctgccat
     6721 atctcagcag tatactcttt ccttcggaat aaagggattg gtaccaggcg gtattttcca
     6781 ggaaaaggag tacaagaaca tttctttgct tgaggagaga gcattactct cccggttgaa
     6841 tgtgctccgg gtatgaccac tcctgataga tagggggggt tgatgccaga ttctgggggg
     6901 tgtccggcaa aacgaagaaa ccagggggca tatccgagag cattatacaa tctgcatggt
     6961 ggaataaaaa ctttcaccta tcgttccagg gatcagggat gatgccccgg cggtgatggt
     7021 actgcggtgc taacttctcc tgtccaccag cctctttggc ctccccttga tccttgagag
     7081 ttccagcccc ccctgttccg taaaggaaaa gaccagggag aaggtgtcac tggagaggcg
     7141 tgagagggct gtcttgtagc ggtaaaaaga tcgtttaaaa ttcgccatca cgagttcttt
     7201 ttcaggtccg gtgatcaccc ctttcgtctc gaaagtgacc ctgaggacgg tatcggccgt
     7261 ctcctcactc tcatagacaa aagcttcgta ttctccggaa aggtactcca tattctctct
     7321 ctggaagacc ccacgttcga tatcgaccct gtttaccggt gttccaagga tccacacggt
     7381 ctctgcctca cgctggggat tcatgatctt catatgggtc ctgccacaag ggcaggtatc
     7441 cctggtggtt acgactgtcg tatcctccgt atcgtaattg atcagcagtg ttcctgtctt
     7501 gcccccgaca ggaaggagtg tcgtcagaac cgctcttccg cattctccgt cctccacaaa
     7561 atcaccgagc gttggatcat acacgtcgag gtggacgaga tcctcaggta catggagtcc
     7621 ctgccgtaca tggcattctc cgcacatcgt cccttccgta cttccatagg tattgaacac
     7681 ttcacggtcc cagacctctg atacgtaggc ccgtgactca tcggcaaaac tctctccccc
     7741 gacaataagc gccctgattg aggattcctc cggcttgatc ccttcagccc tcatccgttc
     7801 tgcaaggcga aggagcttga agacactccc cacgatcccg gtcgggtggt agctgttgat
     7861 gattctggct ggaaaattgc attttccaat ggggatgatg gtagcatgga tgtcgcgggc
     7921 agcaagcgtc atggtgttcg cgccgacgtt cataccatat gacgcgcaaa ttacgatcct
     7981 gtctccatgg gagaaaccct gggaaacgaa actcctggca tatttttccg cgtacctgag
     8041 ccagtcttcc catgtgagaa agaaactctt cgggatccct gatgttcctg aggtctcatg
     8101 gatggtgaag acgtcgctcc actccacgct ccggaacatg aactcttttg tgacaggggg
     8161 ttggttctcg cggatggtcg atccggatat caagggtagt ttgatcagat cctgatgggt
     8221 ttctaccgag gcgggatcaa tccggtgctt ccggaaccat gcatgataaa agggtgaatg
     8281 ttctgcagca taccggatag tgtaccgaac cctttcatca ataagagcat tgaggtcatc
     8341 gcgtgggaga cactcaactt cttccctgaa aaatccactc tccaccattt tcataccatt
     8401 acctttcatg ctaatcatat atgttcgtgg actctgtctg aactatcagt cgctcatatt
     8461 tacaaaaaat ctcgctaccg ctctccactc tgcgaaaatc ccctcttctc ccaatagcca
     8521 cggtaggatg gatcatcaga cacctcgatt ctcgttaccc atttgatcca tttgtaaccc
     8581 catcgatctt ccgccacaag ctggaaggga aaacctctct cggcaggaag tgtaacgccg
     8641 ttcatggaat atgccaggag aatatcgcgg tcctcaatat agtcaagagg aagagacgtt
     8701 gaataaccat ctgctgcata aaatatcact gtgttagcgg caggattcac tccagcctcg
     8761 ttgagcaaat cttttaccct caccccctcc cagagtattg tcacttccca tccttcaaca
     8821 cagaagaggg tgactttttt ctggaaggga gggtattctg tgatcacctt ttcataggga
     8881 aggagaagag ggtgcgatac aagcccatta atctccagca ggtacgctga ctggttgatg
     8941 tactgggggc cttcgagaga attttctcga aaatcggtga ttgacgagag acgctccccc
     9001 tgatactccc ttatttccac tgaggtcagg gcggtaacgt ttgcatcatc cctttccggg
     9061 agtaattcag gattgaaaac acaacctgaa aggagggtag taagtatcag gataagtgct
     9121 atgataccag aaggagtcat tctcatttta tcccccgtct gtcattgggc tctgattctc
     9181 ttaagggtgc ttaacgaggc atgtcaggaa caataacagc cctgttccag aaaaaataga
     9241 tttatctggt ttgagatgag ttcctagagt gaaggagaag aaacatgccg ccagtcgagc
     9301 gatacaagtg cagggtctgc ggatatatct attcaccact gcggggagag ccacataacg
     9361 gcattcctgc cggtaccaaa tttgacgatc ttcccgagtc atatatctgc cccatctgcg
     9421 ggatgcaggg gaaggggaag attggaaaat ggggatttga ggagtgggtg ccaacacgct
     9481 gggtttgctc agtctgcggg tatgtctacg accagaagag gggcgagccc caccggggaa
     9541 taaaggtggg gacggccttt gaggatctcc ctgaggatta tgtctgtccg gtctgtgccc
     9601 tcgatccaaa gataaaagtc cagttcggaa aagtctttaa aaatggcttc gaaccacttg
     9661 agctctgaag ccagagcact tcgtttttag aatcctttcg gtctccgggg gtccgttctc
     9721 acagcctcac tgatcgaggg gtatgatccc aggtcaggcc atatcctgat gaaaaacacc
     9781 tcctatttca gcttcttcag tgcctcccgt gcggtatgac ggacctccag ttcctcgtcg
     9841 tcaagcagcg gttccagata ttccctgtat cgtatctcgc cgatctttcc cagggcttca
     9901 gcagctgatt gtctcacccc tttttttttg tcggtcagga gtgggatgat caccggaccc
     9961 gccttgggat caccaagttc ccccaatgcc catgcagctc cctggcgggt ccaccggtcc
    10021 ggactgtcaa tgcaccggat gagtggttca aatgcccggg gatcccgcag ggttccaagc
    10081 gccctgatag ctacccagac gacatctgga acctggtctc ccaggagaag aataagaggt
    10141 tccaccgcac gttcgtcacc catgttcccc aatgactctg ctgaccgggc gcgaagggtg
    10201 gaattgggat cggaaagttg ttttatcagg atggaaaggt tctgttcctg ccgctcctca
    10261 tggcgcatca tcgcctctct cacaggatcc cgtccggtac catgcatttg tatgccctca
    10321 tcatgtgctc tgtagcttcg ggcatcaatg tttctgtttt cccccacgac gcctcatgaa
    10381 aatagtgtgc tattaatagc atcaggtcga tctaacagaa tacacctggt aacagtcgag
    10441 ggcaaccatg tctcacctga tcctgcttcc taccgatttc tctgaatatt cccggatgac
    10501 agccgatatg gtgtcaggta ttccgggtga acatgaaata atcctgctcc atgtggctga
    10561 ggggggttcc gtgtcgacac ggagaatcat tggtaatcac caaccctcat cgagttctgt
    10621 tgaacagagc ctggaggcag agaaagtgag gctcgtggca agggggaata cagtccgcac
    10681 catcatcctc aaagcggacg ggcgggacat ccctggatta attctctcgt acgcacggaa
    10741 agaacaggtg gatctcatca tgctgggagc ccgtgggaaa ggggttgtag aaggcctgct
    10801 tctcggttct gtctcatcga agattgtccg taatgccaca actgatgtcc ttattaccca
    10861 tcacccgggc ctggctcttc cctttcaaaa atcaccagaa ccgaagacga accaaacgcc
    10921 cctgttttca agagttctgt atccggtgga cttctcaaaa ccctgcctcg agaggttgtc
    10981 ctctctcatg gcccggagct tcatcgatga gatcgttcta ctcaatatta tcccgaatgc
    11041 agacaatagg aaggagctcg atcaggcaat cagcggagcg tatctccaac tccaggatct
    11101 tgggaagcgc tacaacgaaa gcagcacaaa ggtaaagctg cttctgcggt tcggaaagcc
    11161 cgcagagatg atatgtacga tggcccttga ggagaaagca accctggttg ctctcccacg
    11221 gttaggggct tcggatttta ccaggagtct cccgattgga agcactgccg aggaagtggt
    11281 aaagaaggca aaaataccgg tatgtttgct ctatcccgat atcacgcttg acgtttcggt
    11341 gcgggagctt gagaaggatg aattcccgct tgcagaaaaa gtgtgggctc attaccatca
    11401 gcagaaggca gactcccgca ctgaccgtat cttcggtgta ttcgttgaag ggatgcttgt
    11461 ggcgattgcc aggtgcaggc gccatccgga cgggctcgaa gtggatggag tcttcaccct
    11521 cgatgacttc aggggaaggg gatatgcacg ggatgtcatg cagccattgg tgtcagcctg
    11581 tgggaatgag atactgtata tgcattccac cctcggcctg gtggaatttt atcgtcagtt
    11641 tggctttgaa aaaattccgg aacagaacct cccgccgacg ataaaggcac ggttcgattt
    11701 tgcgatgggc gagatgcagg gatccaatgt tcagcctatg atgaggaggg caacgggcca
    11761 gtgatgctcg tctgttgaaa ggtgagaaat tagccacttt ataggctttt tttaaaaagg
    11821 aatgaatccc tcgaaaaaaa gggatcagat gagtttgaag atggggtggg tttctgcctt
    11881 gacatcgatg ccgagcggcc gcacagcttc gagtgtgacg atgtccatgt aggcttgggc
    11941 cgtgatcatc ggctcgacgt tctcgatcgg accgtacatg ataaggtcgg cgccaagcgt
    12001 gcttgcgaca aggttgcagc cgatatccgg agctgaccag gcagcctgga taactccgtc
    12061 aaggccgccc ttgtagtggt gaagcagctg cttgagatac acatccttgc ccttcaggga
    12121 ctctgcgagt gcggacggat tcttcttcga tcccttccag cgcttgagcc atgtccacgc
    12181 aacggtcatg ttgtggtagg cgccacccgt cgggtatcca tgaatagcct tacaggcgag
    12241 gatctcacgg tatgcacttc ccgagccaag tccgagtggt gttgcggcgg tatccaggat
    12301 aggccgggtg ataccacact cttcggcaat ctggatcatt ccttttgtct gccctgcaac
    12361 gcctccttcg acgagtacct tctcccttcc tgcgacggag ggatcggcgg ggttgaaggc
    12421 caggacaatg gctgagttca catcactgtt tttgagcgct tcgatattct cgggcataat
    12481 cgaaccattg atagagttgt aaatggcccg gtttgccact ccggtatcgg ttacgtactt
    12541 acaggcatgt gccagggcgg ccggcacaga ggagtccata aggaatgcag tcttgctgtc
    12601 aagactgcag aaccagtcga tgtagctctc gaatgctgcc gggtattcag cgaggatctg
    12661 gataaagtgc ggaatcccca caagatcaga gagttcctgg cacctgttcc agagcgcttc
    12721 tgctgctgct ttgtctatct ttccggtctt gtcatcaagc acgatctcat gcttgttata
    12781 gaagattgag caagccagta cagtcgggta ttcaccaggc tgtcctccga tcttggtgcc
    12841 gttgaagtcc cagacttgct gctctttttc gaatctgaac atgttctttc acacctccat
    12901 tatagtaaga cgtttagttt cgggagcagt accaggagca ggacaaatac ggtgagccct
    12961 gccaccagac catagaggat accaatatcc cgaccgattt ttcttccgac gcgctgggcg
    13021 atctcggcat ctgcaaactc gatctgtttc tcgatcttgt ccagacgctt ctcgatctca
    13081 aggaactggg gatttacccc tgctgctgcg gtctctacgc caccagcagc ttccttgacc
    13141 tcgacaacca ttgggtcagc agcgaacgca cccgggtctc tccccttgag ttcgttgatc
    13201 ttggccccga tagtgccgag atcttcggac tccatgatgt tgacgagttc acactgctcc
    13261 tggaagcgct tgatagcatc atcggcaagg ttctcgatga atggaatggc accttcagcc
    13321 ccgacgactc ttccgtcctt gaccccgcct ttatggagtg cagccatggt ctggcctgca
    13381 aggtgaccct tcacctcagt cccgcagagc aggaggaaac ggatattcgg gttggagatg
    13441 atgttagcga tgaccttctc gagaccaagg ttttcggtct tacaggaccc gcagatagcg
    13501 gctcccttgt cgcagatccc cttctcatcg aggtgggaac cgcaggtcat gacggcgatc
    13561 gggctgttgg catcgcccgc atggaagtct ccctggacga tcggccaccc gcttgccggt
    13621 gatttcttgt ctgccatttt tctcacctca catgaacatt gcaggtacca cgatcaggac
    13681 gagagaaacg accagtccga tggcgaatcc aatgataccc atctcggaca ttgccgaatc
    13741 aaacttgttg gatcgggcga tcagctgcga cttgtagcgc atgttctcca ccatcctgtc
    13801 gatggctacc atcctgatgg gtcctgctgc cacttttgct tcttcttctg ccatttcata
    13861 tcaccccatg agaatgaacc cgagaagggc gaatgagacg atcaggccaa tcatgatacc
    13921 ttcgatcttg cctgaataaa cgccggatgc aaacttgtcg cggtacccga tgtctgtcac
    13981 catcctctcg atgatcttca tccttgcatg gatcagtgcc agttcaccgg acatgggctg
    14041 gacgacccca cctgcttctt ccgcacctcc ggctgcttcc ttgacctcga caaccatcgg
    14101 gtcagcagcg aacgcacccg ggtctctccc cttgagttcg ttgatcttgg ccccgatagt
    14161 gccgagatct tcggactcca tgatgttgac gagttcacac tgctcctgga agcgcttgat
    14221 agcatcatcg gcaaggttct cgatgaatgg aatggcacct tcagccccga cgactcttcc
    14281 gtccttgacc ccgcctttat ggagtgcagc catggtctgg cctgcaaggt gacccttcac
    14341 ctcagtcccg cagagcagga ggaaacggat attcgggttg gagatgatgt tagcgatgac
    14401 cttctcgaga ccaaggtttt cggtcttaca ggacccgcag atagcggctc ccttgtcgca
    14461 gatccccttc tcatcgaggt gggaaccgca ggtcataacg gcgaccgggc tgttggcatc
    14521 gcccgcatgg aagtctccct ggacgattgg ccacccgctt gccggtgatt tcttgtctgc
    14581 catttctttc acctcagatc agatggaata catagattcc tgctatgaag agccctactg
    14641 ccagtccgta ccagaacgcg gtcattcctc cggccattgg cagggtgttc tcccttcccg
    14701 ggaacgaggc agcaaagtta cctgttccgg aaagcatgtt cacgatgtca tttgcaaccg
    14761 tctcaagctc agcgaccttg tcgatgacag gtgccagtga ctcgccagcg gtggtgacaa
    14821 cccctatcat gggatccata acgagcccat attcgggaag aacttgaacg tacatcagtg
    14881 tccctccacg tctgggattg gttttgactc aagccaggca gcagcatccc tctttgagag
    14941 ttcgatgtac ttggcatagg tgtaatacca tccaatgata gcaacgaaga gtgacaccaa
    15001 tgcagcgctc atcgaaatca gcggcattga cattattgca gccgtgatca tggagaggaa
    15061 cccgcactcg gcagccagca tcagcatcct gtcctgggtc cagttgg
//
LOCUS       DAOOCO010000021        15101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3567_length_15101_cov_4.869932, whole genome shotgun sequence.
ACCESSION   DAOOCO010000021 DAOOCO010000000
VERSION     DAOOCO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3567_length_15101_cov_4.869932"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..313
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..50
                     /rpt_unit_seq="atttcaatccacgcacccacacggggtgcgac"
     gene            complement(536..826)
                     /gene="cas2"
                     /locus_tag="PK154_02295"
     CDS             complement(536..826)
                     /gene="cas2"
                     /locus_tag="PK154_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPW09924.1"
                     /translation="MMILVTYDVNTETPEGKKRLRQVARECLNFGQRVQNSVFECLVD
                     PTQFAKLKHTLCGIMDYDKDSIRFYYLGSNWQNRVEHYGNKKGYNPEGLLIT"
     gene            complement(837..1883)
                     /gene="cas1c"
                     /locus_tag="PK154_02300"
     CDS             complement(837..1883)
                     /gene="cas1c"
                     /locus_tag="PK154_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HPW09925.1"
                     /translation="MGLIIMRKLLNTLYVTTPDSYLHRDGENVVVKVGNEERFRIPIH
                     NLEGIVCFGYMGASPHLMGLCSDNNVSLTFLTPHGEFLGRVNGRVRGNVLLRRTQYRT
                     ADDLQASQNIAKCFITGKIVNCRTILARSLRDHPNVVDCNKIRYADSMLIENLLKVET
                     CHTSDTLRGIEGNCAKFYFDVFNELILKQKDFFFLSERNRRPPLDNMNALLSFLYSLL
                     SHDVESALETVGLDPYVGFFHTDRPGRPSLALDMMEELRPFISDRLALNLVNLQQISK
                     DDFLRKEGGGIILTENGRNEVLKAWHKRKQDEITHPFLGEKISIGLLPYAQALLMARF
                     LRGDIDGYPPFFMN"
     gene            complement(1916..2599)
                     /gene="cas4"
                     /locus_tag="PK154_02305"
     CDS             complement(1916..2599)
                     /gene="cas4"
                     /locus_tag="PK154_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPW09926.1"
                     /translation="MTKSRYADDDLLSLSGIQHFRFCKRQWALIHIERQWIENLRTQE
                     GKFVHELTDDPYFHESRGNFIITRAVPMVSYSLGLYGVADIVEFTRSEKGVPIPDLEG
                     FWLIKPVEYKRGKPKIDERDEVQLCAQVICLEEMFGIRINEADFFYHEVRRRQSIPIS
                     QGLRTLVKTLADDMHDVFKKGITPKAEVGKRCYLCSLSDVCVPKLTKKKTSVRKYIGN
                     HVGEACIRD"
     gene            complement(2596..3453)
                     /gene="cas7c"
                     /locus_tag="PK154_02310"
     CDS             complement(2596..3453)
                     /gene="cas7c"
                     /locus_tag="PK154_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HPW09927.1"
                     /translation="MSEPIKNRYEFVLLFDVENGNPNGDPDMGNMPRVDPQTGYGIVT
                     DVCLKRKIRDYVDMVRTGKEGYEIYVRDGVVLNEQHTRAVDKIKPKDPKKPKRDEELK
                     LTKFMCDTFFDIRSFGAVMTTEVNCGQVRGPVQLTFARSQDPIFQQEVTITRQAVANA
                     KDADKGKTMGKKQIVPYGLYRAEGYVSAHLAKKTTGFSEEDLNLLWDSLINMFEHDHS
                     AARGKLSAKKLIIFKHDSELGCCQSHILFDKIKVERISSDLPPRSFNDYKVTIDEDIP
                     KGVQVIERL"
     gene            complement(3450..5354)
                     /gene="cas8c"
                     /locus_tag="PK154_02315"
     CDS             complement(3450..5354)
                     /gene="cas8c"
                     /locus_tag="PK154_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HPW09928.1"
                     /translation="MPERRCLLVIIHSLVRYYDILSADTNVKIARPGYSPANVSFILV
                     ISDTGELTNIVDIRADGKKKRSRVLYVPYQESRTSGISPYFLCDKAQYIFGLEHQETY
                     GKKQNSIIKDVTQVLEETGMGRIVITRRSLESMKKFREFHHFLLDSVNDPAVEAFLKY
                     LDTWKPDMAYHHPKIAEFKDDLFAGALFVFEYHGECLHKNVNVKSVWEHHLAMGGSEG
                     TTEMQCLVSGKIEPVSRLHKWIKGVKGTQAQGAPLISFNNESFCSYRRDQSYNSPISA
                     RAMFKYTTVLNHLLEWESKNRIQIGDTTVVFWAETEKTAYENLVLALLDPDNEVDEII
                     DESETVPREKDLRTRQLVNDILQKVKTGHFFEEKDLGIDPETTNFYILGLSPNQGRLV
                     VRFWHQDTFGNFITRLARHHIEMEIEQNDSVRRNVSMKWLLKMTVPRNSDDKTISPLL
                     GGLLMKSILNDTPYPIPMYNAILNRVKVERSINYPRAGFIKACLIRRARARGNHEEDM
                     ITVSLNKESTDIPYRLGRLFAVLEKTQSDSNKDLKSTINSKYFSSASTTPAVVFPVLL
                     KLAQHHITKSEWGFKSNQWIEEIVSDIDEFPAYLNLEDQGKFMLGYYHQRNAFFKKRE
                     ESEQKEEPEL"
     gene            complement(5327..5962)
                     /gene="cas5c"
                     /locus_tag="PK154_02320"
     CDS             complement(5327..5962)
                     /gene="cas5c"
                     /locus_tag="PK154_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HPW09929.1"
                     /translation="MWGDYACFTRPEMKVERVSYDVMTPSAARGILEAIYWKPAITWV
                     IDRIHVMKPIRFDNIRRNEVLGKIPLARVKSTFHGKEAFLFKDANDDRVQRSSLILRD
                     VEYYIEAHFNMTGKAGPDDTVEKHYNVALRRMRKGQCFHHPYFGCREFPVQFKFVEEE
                     IPRSSLTGKINLGLMLWDIDFAHEMNPMFFRAEMNDGVIDVQECLKGGAFS"
     gene            complement(6000..8177)
                     /gene="cas3"
                     /locus_tag="PK154_02325"
     CDS             complement(6000..8177)
                     /gene="cas3"
                     /locus_tag="PK154_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPW09930.1"
                     /translation="MYYAHSKSTLEKSEWQLLKDHLTNVSNIAAGFATDFHAGELAYT
                     GGLLHDIGKYSRDFQMRIRGANIRVDHSTAGAREAELRYPRYFSIILEYIITGHHGGL
                     LNYGSNESGLLERLKKQHLPEYTAFKEELKVPDLQSFKPHLKPFPGRIGFAISFYTRM
                     LYSCLVDADSLDSEQFENPDKSLLRSQYDSMGVLSLRFDEFMKEKISGAANSEINQYR
                     RLIYEQCLDKADHPPQMFSLTVPTGGGKTLSSMAFALEHLKKYDLKRIFYVIPYTSII
                     EQNAKVFRDIFGSKNVLEHHSNFDPKTIDIEEMDEMESLKLSSENWDMPIIITTNVQF
                     FESLFSNRRSRCRKLHNLAQSVIILDEAQMLPTGYLKPCLAALSELVRNYGSTVVICT
                     ATQPNLDGLLDKSVRPLEIITSHEQLYETFKRVRVTEIGRLGDTELSERLKMHRQVLC
                     IVNTRNHAKKLFDSIKETGNCFHLSARMCPVHRRKKIVEIRKVLNTGNDCRIISTQLI
                     EAGVDIDLPTVYRAMAGIDSIAQAAGRCNREGKEPFGDLYVFKSMEPYGGATSWQNLT
                     AEVGEMVFGKESDPLSLSTVSAYFEQLFKYKGGGGLDEKRILLAFEERSNDLAFPFED
                     VGNIFNIIDGGTRDLIVPYDGKAESVIQELRKTESPWSYVRELQGYHVSVYPNEFMEL
                     ERLNAIDLVGDRFYVLKSRDYYSDETGLISSTEIGTDGSLLIV"
     gene            complement(8371..9756)
                     /locus_tag="PK154_02330"
     CDS             complement(8371..9756)
                     /locus_tag="PK154_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPW09931.1"
                     /translation="MVDLSFLIDPVYIATIIIFIIGLQRMSHPLTARSGIVWAGAAML
                     IATLVTFLTPELTNFALIAIAIVIGGGLGYIAAKRVAMTDMPQMVAIYNGMGGGAAAG
                     IAAVHLLGTTTPATSTLAVIGGLIGAVSFSGSMIAFMKLQGWMRPRPITFPGQQVVNM
                     AVLGLAALSGILLLWQPAFLPLSFSTYLPLFFILSLAFGILMTLPIGGADMPVVISMY
                     NAFTGLAVGLDGFSFATPNYAMVIAGIIVGAAGTLLTVLMARAMNRSIANILFGAFGA
                     TEETTAEIQGSIKSIEAEDVAAMLAYANSVVIVPGYGMAVAQAQQKVKELMDQLESRN
                     VTVKFAIHPVAGRMPGHMNVLLAEAGISYDHLFDRDEINQEFPGTDVVLVIGANDVVN
                     PAAHREGSPLFGMPILDVEQARNVIVLKRGQGRGFSGIENDLFYRDNTRMLYGDAKET
                     LGELVQAVKRL"
     gene            complement(9758..10066)
                     /locus_tag="PK154_02335"
     CDS             complement(9758..10066)
                     /locus_tag="PK154_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPW09932.1"
                     /translation="MELSLFWTSIYIVILAAFTGYVVISRVPVILHTPLMSGSNFIHG
                     IVLIGAMVALGLATTTGQQIIGFIAVVLGAANVTGGYVITERILQMFDSSRKKPGKGD
                     "
     gene            complement(10066..11208)
                     /locus_tag="PK154_02340"
     CDS             complement(10066..11208)
                     /locus_tag="PK154_02340"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPW09933.1"
                     /translation="MPFVIAVPKEKAEGERRVAIVPEVVQKLTRAGHEIRIETGAGTG
                     AYYPDDLFSGAGAKVVSGRAELFQDARVVLSVQPPAPDDIDQLAEGSIVIGFMNPSRN
                     PESITRMRDRKVTAFALELVPRITRAQSMDALSSQATAGGYAATILGAAHCPKFLPML
                     TTAAGTIRPATVLILGAGVAGLMAIATARRLGALVEAYDVRRAAGEQVRSLGAKFLEL
                     EINAEGQGGYARELNAEEKVQEQQMVSAAVARADIIITTAAIPGRKAPVLITKETVLT
                     MKPGSVIVDLAAESGGNCELTQAGKTVTEHGVTIIGPANLPAHVAFHASQMYAKNLQQ
                     FLALLIDKEGSLVSEFSDEILAASLMVHAGEIRHAPTRELIQGGRS"
     gene            complement(11262..12905)
                     /gene="arcS"
                     /locus_tag="PK154_02345"
     CDS             complement(11262..12905)
                     /gene="arcS"
                     /locus_tag="PK154_02345"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HPW09934.1"
                     /translation="MSTFEVKKRDGLARIAVYSRGEQEIRLPCAMDTEILFPDLADRA
                     FSNVPLAAPQSFASAWLSPGKDQPVLVHPAIPPSVSSGDCVMAGNWNSVLDNPRGYTD
                     WLVRLKEQIPSDSAWYAPGAALPSNVHLLCYSGFDLFDFIAVDLQTARHRFCLPEGEF
                     PASVMDTGVCGCTGCRDGDLGMHNRLALQRELGLVSYFIGDQQLREFVEARSRMHAAH
                     VAILRHLDRHYPFLEQHLPVARACRLGAMSGEALKRPEVVRFADRVLHRYLPPAADVA
                     VLLPCSARKPYSASQSHRKFAEAIGGRALELIVTSPLGLVPREIERIYPAAHYDIPVT
                     GYWDREELAFTAGIVADFLERHQFRRVIAHLEGGALEAARLAAARAGIMMEESGDPRP
                     VSGSALRNLSSLLDGERPVRQDPVRGTLSWQFGIPVDTQGMSVRWKPPHLMVRRGKEP
                     LFSLDPGTGFLRPTFQGWKYLPGVYRVRIDDFVPQGDILAPGVLSVDPAVREGDEVLV
                     EGPHALATGRAAMGAAEMQSSSRGVAVRVRKVLKGQTFH"
     gene            complement(12902..14347)
                     /gene="tgtA"
                     /locus_tag="PK154_02350"
     CDS             complement(12902..14347)
                     /gene="tgtA"
                     /locus_tag="PK154_02350"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HPW09935.1"
                     /translation="MAITFEVLEKDIAGRIGRLKAGERTVRTPLLLPVINPHLQPVSP
                     AEMKAMGAEGIITNAYIFSRSGEYRDEALARGLHDLLGFDGLIMTDSGSFQLSVYGDI
                     AITNLETLQFQKAIGSDIHVPLDIPTPPDANRQRASAELAVTMARLREAKEVFGPDAP
                     LAGPVQGGIFEDLRMKAGREAGDLGFSFCPIGAVVPLMESYRYPDLVRVVMAAQRGIP
                     RSSCVHLFGAGHPAMFALAVAMGCDVFDSAAYALYAKDRRYLTAQGSYRLDELAEFPC
                     ACRVCRSHTVQEVREADDVVRLLSLHNLAVSLAEISHVRQAITEGTLWELVDDRCRSH
                     PQLLRGFRELLHFRESLAREDRVTKHRFFYRGEESCARTEVVLYQDRLSRLPVSGTVL
                     IAMDGITREGYDTVLFFKPPFGPYHAALRETFPIGQSEIPDWDSAMVRQGCRGIARII
                     SENPGSRFVIYTPREWCDLVCHELPGTEVQW"
     gene            complement(14510..>15101)
                     /locus_tag="PK154_02355"
     CDS             complement(14510..>15101)
                     /locus_tag="PK154_02355"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991156.1"
                     /note="biodegradative; catalyzes the formation of agmatine
                     from arginine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPW09936.1"
                     /translation="LQTRGIVIEKSGDYTVLFLFSMGNTKGKWGTLLTELFEFKRLYD
                     EKAPLEEIFPDLTGRYPHRYGGMTLPGLADQMHAFKNDNRMCELLHGAFSLLPRPAIT
                     YAEAYRRLVRGEVEQVSVADAGRRIVATGIVPYPPGIPLLAPGEETGEEGGALLKYLK
                     ALEDFDRCFPGFEHRIHGIEKRAGEYVMYCIKGERG"
BASE COUNT         3592 a         4042 c         3743 g         3724 t
ORIGIN      
        1 tgggactagt gcatctagat ttcaatccac gcacccacac ggggtgcgac cttgaaacaa
       61 ccgggattgt ggcattcatc gcatgatttc aatccacgca cccacacggg gtgcgactta
      121 tggggatgtt gatcggttcg ggtatggggt atttcaatcc acgcacccac acggggtgcg
      181 actgcaaact atgtccccgt cccggtcaat ctctccattt caatccacgc acccacacgg
      241 ggtgcgacgg tattttccag ctttaagaca aagttcctcc aatttcaatc cacgcaccca
      301 cacggggtgc gactgcaaaa ttcagatcta catttactct gaattaaggc atataatttc
      361 atcattttca ggttaattga attttactgt tctgattagg tgagtggatg aatctaccaa
      421 ataatgttta ttatttctca ttttacgaga gatctgcaaa agacataaat atcagataac
      481 atattttccc gcgaatattc cgaggaggta ttggtcactt catattcgca tccattcatg
      541 ttattaaaag tccttcagga ttgtagcctt tcttgttacc gtaatgttcc actcgattct
      601 gccaattact cccaagatag tagaaccgaa tactatcttt atcataatcc attatcccgc
      661 atagtgtgtg tttgagtttt gcaaactggg taggatcaac cagacactca aaaactgagt
      721 tctgcacccg ctgtccgaag ttcaggcatt ctcgtgccac ttggcgaagt cttttctttc
      781 cttctggtgt ttctgtattc acatcatagg ttactaaaat catcatagcg atcacctcaa
      841 ttcatgaaga atggcggata tccatctata tctcctcgta agaaccgtgc catcaaaagt
      901 gcctgtgcat acggaagtag tccgatcgat atcttttcgc ccaagaaggg atgagtaatt
      961 tcatcctgtt tcctcttatg ccaagcctta aggacttcat ttcgcccatt ttctgtcaat
     1021 ataatcccac ccccctcttt tctcaaaaaa tcatccttac ttatctgttg aaggtttaca
     1081 agattcaatg cgagacgatc ggaaatgaaa ggacgaagtt cttccatcat atcaagggct
     1141 aggcttggtc tcccagggcg atcagtatgg aagaatccaa cataaggatc caatcctacc
     1201 gtctccaagg ccgactcgac atcatgggag aggagagaat agaggaatga taacagggca
     1261 ttcatattgt caaggggggg ccttcggttt ctctctgata ggaagaaaaa atctttttgc
     1321 ttaagtatca attcattgaa aacatcgaaa tagaattttg cacagttccc ttcgattcct
     1381 cgaagggtat ctgatgtatg acatgtttca actttaagaa gattctcgat aagcatacta
     1441 tccgcatatc ggattttatt gcaatcaaca acatttggat ggtcccgaag acttcgggcg
     1501 agaatagttc gacaattgac aatctttcca gttataaaac attttgcaat gttctgggat
     1561 gcctggagat cgtccgccgt gcggtattgt gttcttctta agaggacatt ccctctgact
     1621 cggccgttaa cacggccgag aaactcccca tgtggcgtta aaaatgtgag actgacattg
     1681 ttgtccgaac ataagcccat taagtgtgga cttgcgccca tgtaaccaaa acagacaatc
     1741 ccttcaagat tatggattgg aattcggaaa cgctcttcat tgcctacttt aacaacgaca
     1801 ttttcaccat cccgatgaag ataagaatca ggagttgtca catagagagt attgagcaat
     1861 tttctcataa ttattaatcc cattcccttt ggttattccc cctgatattc ttcttttaat
     1921 cacgaatgca tgcctcccca acatgattcc ctatatattt ccggacactt gttttctttt
     1981 ttgtaagttt cggcacacac acatcggata aagaacacag gtaacacctc tttcctacct
     2041 cagcctttgg agtgataccc ttcttgaaaa catcatgcat atcatctgcc aatgttttca
     2101 ctagagttcg caaaccttga gaaataggaa tcgattgtct cctacgcact tcatggtaaa
     2161 agaaatcggc ctcatttatc ctaattccaa acatctcttc cagacatatg acctgagcac
     2221 agagttgcac ttcatcacgt tcatctatct taggttttcc cctcttgtac tcaacaggct
     2281 ttataagcca aaatccttct aaatccggaa tcggtacgcc cttttccgag cgggtaaact
     2341 caacgatatc ggcaactcca tacaatccca gagagtaaga taccatagga accgccctag
     2401 taattatgaa attgcctcgg ctctcatgaa agtatggatc atcagtcagc tcatggacaa
     2461 attttccctc ttgagtgcgg agattttcaa tccactggcg ttcgatatgg atcagagccc
     2521 attgacgctt gcagaatcga aaatgttgta tgcccgacaa cgagaggaga tcgtcatctg
     2581 cataccttga tttggtcata acctttcgat gacctggact cccttcggaa tgtcctcgtc
     2641 tatcgtaact ttgtaatcgt tgaaagagcg agggggtaaa tcggaagaaa tgcgttcgac
     2701 ttttatttta tcgaacagga tgtgtgattg acaacagccc agctcactgt catgtttgaa
     2761 aatgatcagt ttcttcgcgg agagtttacc tcttgcagct gaatgatcat gctcgaacat
     2821 gttgataagg ctatcccaca acagattcag atcttcttca gagaacccgg tagtcttctt
     2881 tgcaagatgc gctgatacat atccttcagc ccgatagaga ccatatggaa cgatctgctt
     2941 cttccccatc gtcttgccct tatcagcatc ctttgcatta gcgacagcct gccgggtgat
     3001 tgtgacctcc tgctggaaga tcggatcctg actccgtgcg aaggtgagct gaacgggccc
     3061 ccggacctgg ccgcaattga cttctgtggt catgaccgcc ccgaaagatc gtatatcgaa
     3121 aaatgtgtcg cacataaatt ttgtaagttt cagttcttca tctctcttgg gcttctttgg
     3181 atccttgggt ttgattttat cgacggcgcg tgtgtgctgc tcattcaata cgaccccatc
     3241 tctcacataa atttcatatc cctctttccc ggtccttacc atgtccacat aatcgcgaat
     3301 ttttcgcttt aggcagacat cggtcacgat gccataccca gtctgcggat cgacacgggg
     3361 catattcccc atgtccgggt cgccgttcgg atttccgttc tcaacatcaa acaacaatac
     3421 gaattcatac cggttcttga ttggttcgct cataattctg gctcctcctt ttgttcactt
     3481 tcttcccttt tcttgaaaaa cgcgttccgc tggtgatagt acccgagcat gaacttccct
     3541 tgatcctcaa gattcaggta tgccggaaat tcatcgatat cggatacgat ttcctcgatc
     3601 cattggttcg atttgaatcc ccattcggat ttggtgatgt gatgttgtgc tagtttcaac
     3661 agaacaggga atacaacagc tggtgttgtc gatgcactgc tgaagtattt gctgtttatt
     3721 gtgcttttca ggtccttgtt cgaatcgctc tgcgtctttt ccagtaccgc gaacagccgc
     3781 ccgagacggt acgggatatc tgtactctct ttgtttagac tcacggtgat catatcctcc
     3841 tcatggttac ctctcgctcg tgccctcctt ataagacagg ccttaatgaa gcccgcccga
     3901 gggtaattga ttgaacgttc gacttttact ctattcaaaa tggcattata catcgggatc
     3961 gggtacggcg tgtcattgag aatggacttc atcagcagtc ccccgaggag cggagatatt
     4021 gttttatcat ccgagttacg aggaacagtc atcttcaata gccatttcat cgaaacgtta
     4081 cgtcgaacgg aatcattctg ttcaatctcc atctcgatat gatggcgtgc gagtcgtgta
     4141 atgaagttcc caaaagtatc ctgatgccag aaacggacaa cgagccgtcc ttggttgggg
     4201 gaaagtccaa gaatatagaa atttgtggtt tccggatcga ttccaagatc cttctcctca
     4261 aaaaaatgcc ccgtcttcac tttttgcagg atgtcattaa cgagctgacg ggttcggagg
     4321 tccttttcac gtggtacagt ttctgattca tcgattattt catccacctc attatcagga
     4381 tctaataagg cgagaacgag attttcatac gctgtttttt cagtttcagc ccagaaaaca
     4441 acagttgtat cgccaatttg gatccggttt ttactttccc actccagaag atgattcaag
     4501 acggttgtat atttgaacat cgctctagcg ctaatcggtg aattgtaact ctgatcacga
     4561 cggtaagagc aaaaactttc gttgttgaaa ctgatgagtg gggcaccttg tgcttgagtt
     4621 cccttaacgc ctttgatcca tttatggaga cggctgacag gctcgatttt accactgacc
     4681 agacattgca tttcggttgt tccttcggat ccgcccattg caagatgatg ttcccatacg
     4741 gatttgacat tgacattttt atggaggcac tctccgtgat actcaaatac aaagagagct
     4801 ccggcaaaga gatcatcctt gaattcggca attttcggat gatggtatgc catatcgggt
     4861 ttccatgtat cgagatattt caaaaatgcc tctacagcgg gatcgttcac ggaatccaga
     4921 agaaaatgat gaaattcacg gaattttttc atagattcca acgatcgccg ggttatgaca
     4981 atacgtccca ttccagtttc ttccagaacc tgagtgacat cttttataat tgagttttgt
     5041 tttttcccat aggtttcctg atgttcaaga ccaaagatat actgagcctt atcacatagg
     5101 aaatatgggg atattccact cgtgcgggat tcctgatagg ggacgtataa tactcttgat
     5161 cgtttctttt taccatcagc acgaatatcg acaatgtttg tgagttctcc tgtgtctgat
     5221 attacgagaa tgaaggatac attcgccgga ctatatcccg gcctggcgat tttcacattg
     5281 gtatctgcag acaagatgtc ataataacgg acaagggaat ggataatcac gagaaggcac
     5341 ctcctttcag gcattcctgc acatcaatca caccatcgtt catttcggcc cggaaaaaca
     5401 ttggattcat ttcatgggca aagtcaatgt cccagagcat caaccccagg ttgatttttc
     5461 cggttaatga agaacggggg atctcttcct cgacgaattt gaattgtaca ggaaactccc
     5521 gacacccaaa gtaggggtga tggaagcact gccctttcct catcctcctg agagcaacgt
     5581 tgtaatgttt ctcaacggtg tcatcaggac cagccttgcc cgtcatgtta aagtgcgctt
     5641 cgatgtagta ttccacatct cgaagaataa gggaagaacg ctggactcta tcatcatttg
     5701 catccttgaa gaggaatgcc tcttttccat ggaatgtgct tttaactcta gcaaggggta
     5761 ttttcccaag cacctcattc cttctgatat tgtcaaacct gatcggtttc atcacatgaa
     5821 tccgatcgat cacccaggtt attgcaggct tccagtatat cgcttcaaga attcccctgg
     5881 ctgcagaggg agtcattaca tcgtagctta cccgctctac cttcatttca ggacgggtga
     5941 agcacgcata atccccccac actttcaatt tgataccaaa tccgatatcc ttcacctcct
     6001 tacacgatca ataacgatcc atctgttcca atttctgtgc tacttatcaa accggtttca
     6061 tctgagtaat agtcacggga cttgaggacg tagaaccgat ctccaactag gtcaatggca
     6121 tttaagcgtt caagctccat aaattcattt ggataaacag aaacgtgata accctgaagc
     6181 tctctcacat agctccacgg actttctgtt ttacgaagct cctggattac cgactcggct
     6241 tttccatcgt agggtacgat tagatccctt gtccctccat cgatgatgtt gaaaatgttt
     6301 ccaacatcct caaatggaaa tgcgagatca ttggaacgtt cctcaaatgc caagaggata
     6361 cgcttctcat caagccctcc ccctccttta tatttgaaaa gttgttcaaa ataagcagat
     6421 actgttgaga gtgagagagg atcactctcc tttccaaaga ccatctcgcc cacttctgca
     6481 gtaaggtttt gccatgaagt agctcctcca tagggttcca ttgacttgaa gacatagaga
     6541 tctccaaagg gttcttttcc ctcgcgatta cacctcccgg cggcctgggc aatcgaatcg
     6601 atccctgcca tcgcccgata tactgtaggc aagtcgatgt caaccccggc ttctatcaat
     6661 tgcgtcgaaa taatcctgca atcattgccc gtattgagga ccttacgtat ctcgacgatc
     6721 tttttccttc ggtgtaccgg acacatcctc gcactcaaat gaaaacaatt tccggtttct
     6781 ttgattgaat caaataattt cttcgcatga ttccttgtat taacaataca aaggacctgc
     6841 cgatgcattt tgagtcgttc tgaaagttct gtatctccaa gacgaccaat ttcggtaacg
     6901 cgtacccgtt tgaaggtttc atacagttgt tcatgggagg taataatctc aagaggccga
     6961 acacttttat caagaaggcc atcaagattc ggttgtgttg ccgtacagat aactactgtg
     7021 gagccgtaat ttcggaccag ctctgataat gcagcaagac acggcttcag gtatccagta
     7081 ggtaacattt gtgcttcatc gaggatgatc acgctctgag caagattatg gagttttctg
     7141 catctcgatc tccggtttga aaagagcgat tcaaagaact ggacattggt ggtgattatg
     7201 atcggcatgt cccaattctc tgaagaaagc ttgagcgatt ccatttcatc catttcttct
     7261 atgtcgatcg tcttaggatc aaagttactg tgatgttcga ggacattttt gctgccaaaa
     7321 atgtcacgga agacctttgc gttctgttca attatgctag tatagggaat gacatagaag
     7381 atccgtttca gatcatattt tttcagatgt tcaagggcaa aagccattga tgaaagggtc
     7441 ttcccaccac ctgtaggaac tgtaagagag aacatctgcg gaggatgatc cgctttatcc
     7501 agacattgtt catagataag ccttcggtat tggttgattt cactatttgc cgcgccagaa
     7561 attttctctt tcataaattc atcaaatctg agagataata cgcccataga gtcgtattgg
     7621 ctgcgaagaa gagatttatc aggattctca aattgttccg aatccagaga atctgcatct
     7681 acaagacacg aatagagcat ccttgtatag aaggagatgg cgaatccaat tctgccaggg
     7741 aaaggtttta gatggggctt gaaagactgc aagtctggaa cttttagttc ttctttaaaa
     7801 gcagtatatt cgggcagatg ctgtttttta agacgttcta aaagcccgct ttcattgctt
     7861 ccataattca acaatcctcc atgatgacca gtaataatat actccaaaat gatgctaaaa
     7921 taccgaggat atcgtaattc agcctctctt gctccagcgg tcgagtgatc tacccggata
     7981 tttgctccgc ggatacgcat ctgaaagtca cgtgaatact taccgatatc atggagaagt
     8041 ccgccagtat atgccaattc ccctgcgtga aaatcagtag caaaaccggc cgcaatgttt
     8101 gatacattcg ttaagtggtc tttgagcagt tgccattcag atttttcaag cgttgatttt
     8161 gaatgagcat aatacatgac ataatccgat aatgatagtt tatatataag ttgatctcgc
     8221 gcaaggtgaa aataaaaaat acttatgaat cattcatttt cggacagggg aatattatcc
     8281 aaaataaagg atacttacta ttcaggaaag gaggtctccc gaaaaaaaag gatttgaagg
     8341 gcagccccct tagcgatatt gatacatctc ttacagtctc ttcactgcct gcacgagctc
     8401 cccgagtgtc tcctttgcat cgccgtagag catccgcgtg ttgtcccggt agaagaggtc
     8461 gttctcgatc ccggaaaagc ccctgccctg gccccgtttc aggacgatca cgttccttgc
     8521 ctgttcgaca tccaggatcg gcatcccgaa gagcggactg ccttcgcggt gtgctgccgg
     8581 attgaccaca tcgtttgccc ctatcacgag caccacatcg gtgcccggga actcctggtt
     8641 gatctcatcc cggtcgaaca ggtgatcgta ggagatacct gcctcggcga ggagcacgtt
     8701 catgtgcccc ggcatcctgc cggcaaccgg gtggatggca aacttcactg tcacgttcct
     8761 gctctcgagc tggtccatca gctccttgac cttctgctgg gcctgggcta ccgccatccc
     8821 gtatcccggg acgatgacca cggagttggc atacgccagc atggctgcca cgtcttcggc
     8881 ctcgatcgat ttgatgctgc cctgtatctc agctgttgtc tcctccgttg cgccgaatgc
     8941 cccgaagagg atgttggcga tcgagcggtt cattgctcgt gccatcagga cggtgagaag
     9001 cgttcctgct gcaccgacga taatccctgc aatcaccatc gcgtaattgg gggtggcgaa
     9061 ggagaacccg tcaagcccga ctgcaagccc ggtgaaggca ttgtacatgg agatcactac
     9121 cggcatgtcg gcacccccaa tcgggagggt catcaggatc ccgaacgcaa gcgagaggat
     9181 gaagaacagg gggaggtacg tggagaagga gagtgggagg aatgcaggct gccagaggag
     9241 gaggatacct gagagggctg cgagtccgag gaccgccatg ttgacgacct gctggccggg
     9301 gaacgtgatc ggccgcggac gcatccagcc ctgaagcttc atgaatgcga tcatgctccc
     9361 cgagaacgag acagccccga tgagaccccc gatgacagca agggtactcg ttgcaggggt
     9421 cgtggtccca aggaggtgga ctgccgcaat ccctgccgcg gcaccgccac ccatcccgtt
     9481 gtagatcgcc accatctggg gcatgtcggt catcgccacc cgcttggctg cgatatagcc
     9541 gagtccgccc ccgatgacga tggcgattgc gatcagggcg aagtttgtga gttcaggggt
     9601 gaggaacgtc acgagggtag cgatcagcat cgcggcacct gcccagacga tcccgctccg
     9661 ggctgtcagg ggatggctca tccgctgcag tccgatgatg aagatgatga tggtcgcgat
     9721 gtagaccgga tcgatcagga atgagagatc caccatctca atcacccttg cccggtttct
     9781 tccggctcga atcgaacatc tggaggatgc gctcggtgat gacgtagcct cccgtcacgt
     9841 tcgcggcccc aaggacgacg gcaataaaac cgatgatctg ctgcccggtg gtggtcgcaa
     9901 ggccgagggc gaccatcgcg ccgatcagca caataccgtg gatgaagttg gagccgctca
     9961 tcaggggagt atggaggatg accgggaccc ggctgatcac cacatagccg gtaaacgcgg
    10021 ccaggatgac gatgtagatc gaggtccaaa ataacgagag ctccattacg atctccctcc
    10081 ctggatcagt tctctcgttg gagcgtgacg gatctcgccg gcatggacca tcaggctcgc
    10141 ggcaaggatc tcatcggaaa attcactgac aagcgatcct tccttgtcga tgaggagggc
    10201 aaggaactgc tggaggttct ttgcatacat ctggcttgca tggaatgcga catgggcagg
    10261 gaggttcgcc ggcccgatga tagtcactcc atgctccgtt accgtctttc ctgcctgggt
    10321 cagctcgcag ttgccgcccg attcagcggc aagatcaacg ataacggaac caggcttcat
    10381 cgttaaaaca gtctcctttg tgataaggac cggggctttc cgcccgggga ttgccgcagt
    10441 ggtgatgatg atgtcagccc gtgcgactgc agccgatacc atctgctgct cctggacctt
    10501 ctcttcggcg ttgagttcgc gtgcgtaccc gccctggcct tcagcattga tctcgagctc
    10561 aaggaatttt gcaccaaggc tcctgacctg ctcaccggca gctctcctga catcgtacgc
    10621 ttcgacgagg gctccgagac gccgtgcggt cgcgatcgcc atgagtccgg ccactcccgc
    10681 tccaaggatg agcaccgttg ccggccggat cgtccctgct gccgtcgtca gcatggggag
    10741 aaacttcgga cagtgggccg cgccgaggat tgttgccgca taacccccgg cggttgcctg
    10801 cgagctgagt gcatccatgc tctgggcgcg ggtgatccgg ggaaccagtt ccagggcgaa
    10861 ggccgtgacc ttccggtcac gcatccgggt gatcgattcg ggattgcggg acggattcat
    10921 gaacccgatg acgatgctgc cttcggccag ctggtcgata tcgtcagggg caggtggctg
    10981 gaccgagagg acgacccggg catcctggaa cagttcagcc ctgccggaga caacctttgc
    11041 gcccgcaccg gagaagagat catcggggta ataagccccg gtcccggcgc ccgtttcaat
    11101 cctgatctcg tgtcctgctc tggtcagctt ctggacgacc tctggcacga tcgccacgcg
    11161 cctctcccct tcagccttct ctttcggaac ggcaatcaca aacggcatgg ccagttctcc
    11221 ctgtacacga taattcctcc ataaacgata gttgcggtat actaatggaa cgtctgccct
    11281 ttcaatactt tccggacccg aactgccaca ccgcgggacg aggactgcat ctcggcagca
    11341 cccatggcag ccctcccggt tgcgagggca tgcgggccct ccaccagcac ctcgtcccct
    11401 tcacggacag cgggatctac ggaaagaact cctggtgcga ggatgtcccc ctgtggaaca
    11461 aaatcatcaa tgcggacccg gtagacgccg gggaggtact tccatccctg gaacgtgggc
    11521 cgcaggaacc cggtccccgg gtcgagggag aagagcggct ccttcccgcg ccggaccatc
    11581 aggtgggggg gtttccaccg gaccgacatc ccctgcgtat ccaccggaat cccgaactgc
    11641 cacgagagcg ttccccggac gggatcctgc ctgaccggac gttcaccgtc aagcagggag
    11701 gacaggttcc ggagcgctga tcccgaaacc gggcgggggt cgccgctctc ctccatcatg
    11761 atcccggcac gggcagccgc cagccgggct gcttccagtg caccaccctc cagatgggct
    11821 atcacccgcc ggaactggtg acgctccagg aaatcggcaa cgatcccggc agtgaatgcc
    11881 agttcctcac ggtcccagta cccggtgacc gggatatcat aatgggcggc agggtagatc
    11941 cgctcgatct cccgcgggac aaggccgagc ggcgaggtca cgatcagctc gagggcccgc
    12001 ccgccgatcg cctccgcaaa cttccggtgg ctctgcgaag ctgaatacgg tttccgtgcc
    12061 gagcagggaa gaagcacggc aacgtcagcg gcaggaggga ggtaccggtg gagtacccga
    12121 tcggcaaacc tgaccacttc ggggcgcttc agcgcctcac cgctcatggc accgaggcgg
    12181 catgcccgtg caaccggcag gtgctgttcg aggaaggggt agtgccggtc gaggtggcgg
    12241 aggatcgcga catgcgccgc gtgcatcctt gagcgtgcct cgacgaattc gcggagctgc
    12301 tggtcaccga taaaatagga gacgaggcca agttcacgct gcagggcaag ccggttatgc
    12361 atgcccaaat ccccatcgcg gcaaccggtg caaccgcaca ccccggtatc catcaccgat
    12421 gcaggaaatt ccccttcagg aaggcagaag cggtgccgag cagtctgcag gtcaacggcg
    12481 ataaaatcga agaggtcaaa gcccgaatag cagaggaggt ggacgtttga ggggagggct
    12541 gcgcctggtg cataccaggc tgagtcggat gggatctgct ccttgagccg caccagccag
    12601 tcggtgtagc cccgcggatt gtcaaggacg gaattccagt tccctgccat gacacagtca
    12661 ccggatgaaa cagagggggg tatggcaggg tgcacgagca ccggctggtc ttttccgggt
    12721 gagagccagg cagatgcaaa tgactgagga gcagcgagcg gtacatttga aaacgcgcga
    12781 tccgcaagat ctgggaagag tatctcggta tccatggcac agggcagccg gatctcctgc
    12841 tctccccgtg agtatacagc gatccgtgca agaccgtccc gttttttcac ctcaaaggtg
    12901 ctcaccactg cacctctgtt cccgggagct catgacatac caggtcacac cattccctgg
    12961 gggtgtatat gacaaaacgg cttccgggat tctcactgat gatgcgggcg atccccctgc
    13021 acccctgccg taccatcgca ctgtcccagt cagggatctc actctgcccg atcgggaagg
    13081 tctctctcaa tgccgcatgg tacgggccga agggaggctt gaagaagagc acggtatcat
    13141 acccctcgcg ggtaatcccg tccatggcga tcaggacagt gcccgagacc ggcaacctgg
    13201 agagccggtc ctggtacagc acgacctcgg tccgtgcaca actctcctca ccccggtaaa
    13261 agaaccggtg tttcgtgacc cggtcctcgc gggcaaggga ttcccgaaaa tgcaggagct
    13321 cccggaatcc cctgaggagc tggggatggg accggcaccg gtcatcgacc agctcccaga
    13381 gcgtcccttc agtgatcgcc tgccggacat gactgatctc ggcgaggctc accgcgaggt
    13441 tgtgcagcga gagcagcctg accacgtcgt cagcttcccg gacctcctgc acggtatggc
    13501 tcctgcagac cctgcaggcg cagggaaact cggccagctc atcgagccgg tagctcccct
    13561 gggcagtcag gtagcggcga tccttggcat aaagggcgta ggcagccgag tcaaagacat
    13621 cgcagcccat ggcgacggcc agggcgaaca tggcaggatg ccctgcacca aagaggtgga
    13681 cgcaggagga gcgggggata ccgcgttgtg ccgccatcac gaccctgacc agatcgggat
    13741 accggtagga ctccatcagg gggaccaccg ccccgatagg gcagaacgag aacccgaggt
    13801 cccctgcctc gcgtccggct ttcatgcgga gatcctcaaa gattcctccc tggaccggcc
    13861 cggccagcgg ggcatccggt ccgaacacct cttttgcctc ccggagcctc gccatcgtga
    13921 cggcgagctc ggccgaggcc cgctgccgat tagcatccgg cggggtcggg atatccagcg
    13981 ggacgtggat atcactccct atcgccttct ggaactgcag ggtctcgagg ttggtgatcg
    14041 cgatatcacc atagacagag agctggaagg atcccgaatc ggtcatgatg agaccatcaa
    14101 acccgagcag atcatggaga ccgcgggcaa gcgcctcgtc ccggtactcc ccgctccgcg
    14161 agaagatgta ggcgttggtg atgatcccct cggcacccat cgccttcatc tcggcaggcg
    14221 agaccggttg caggtgcggg ttgatgaccg ggaggaggag cggtgtccgc acggtccgct
    14281 cccctgcctt caggcggcca atccttcccg cgatatcttt ctcaaggacc tcaaacgtga
    14341 tggccatggt atcccagaaa tttctgcatt aggtagggtt ccggggagca aataaggtat
    14401 ggctctggaa aagggctcag ggatctcctc cggcaaggtc ggcactacaa acaaggaaca
    14461 gcaatgtccc caggtcctgg tgcagcacct tccctgccgg gaacgctgat catccccgct
    14521 cgcccttgat acagtacatg acgtattcac cggctctctt ctcaatgccg tggatacggt
    14581 gttcgaatcc cgggaaacaa cggtcaaaat cctcaagggc ttttaagtat ttgagcagcg
    14641 ccccgccctc ctcccccgtc tcctctccgg gggcaaggag tggaataccg ggaggatagg
    14701 ggacaatccc ggtggcaacg atccgcctgc ctgcatcagc aacgctgacc tgctctacct
    14761 ctcccctgac cagtctccgg taggcttccg cgtaggtgat ggcgggcctt ggaaggagcg
    14821 agaaggcccc gtggaggagt tcacacattc tattgtcatt cttgaacgca tgcatctgat
    14881 cggcaagccc cggcagcgtc atcccgccgt accgatgcgg gtaccggccg gtgagatccg
    14941 ggaagatctc ctcaagaggg gccttctcgt catacaaacg cttgaactcg aagagttcgg
    15001 tcaggagggt tccccacttc cccttcgtat tgcccatcga gaagaggaac aggaccgtgt
    15061 aatccccgga cttctcgatc acgatccctc tcgtttggag g
//
LOCUS       DAOOCO010000022        14640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3743_length_14640_cov_4.567021, whole genome shotgun sequence.
ACCESSION   DAOOCO010000022 DAOOCO010000000
VERSION     DAOOCO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_14640_cov_4.567021"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PK154_02360"
     CDS             complement(<1..517)
                     /locus_tag="PK154_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW09937.1"
                     /translation="MTVSSLLHALSEDPSFSRHIFTVKEFYRSDGEYGTCSLSSRIST
                     YLNTRGIRLYRHQAAAMDAAMSGENIVLATSTASGKTLAFLLPVFETLGRDPESTALF
                     LYPTKALANDQLKAVQELEKGTGIRVSPALYDGDTPADKRPRIRRSARIILTNPHELH
                     QILPWHHQWSSF"
     gene            complement(514..1590)
                     /locus_tag="PK154_02365"
     CDS             complement(514..1590)
                     /locus_tag="PK154_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPW09938.1"
                     /translation="MESGHVYPSISRQWRSRIGESHDYAVIRNDSIFRTGFHDRYVFE
                     SLHTQATAQLHAAMATYGDKSLDEIFHGRECDTYEGTCYCIPSREPLDLKGINKAAIR
                     STLLSDLTLVYGIGRRKERELKARGYATIVDLAVHRRYGKAARETMAILSGHEIQPIE
                     QLISRWLSPSHPSCLLTAGFYHPRDLIFIDIETLGLFSRPIILFGLAEVRSGTLFISQ
                     YLLRDINEEIPALSVVMDQLEEDRVLVSYNGKAFDIPYLAGRAAFYGRPAVIGNPHYD
                     LLHFSRRRWRGELPDCRLVTLEQRILGTGREDDIPGAMVPEFYEAFLTTGNPGPLVPI
                     VTHNRQDLVSLARLFCFLQGECVS"
     gene            1797..1976
                     /locus_tag="PK154_02370"
     CDS             1797..1976
                     /locus_tag="PK154_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09939.1"
                     /translation="MFQRLLDQAGYYNCSLYKTGKVCCRELISGGLESVSNEEECPWF
                     SNEKKRVRSYRVAAI"
     gene            2054..2848
                     /locus_tag="PK154_02375"
     CDS             2054..2848
                     /locus_tag="PK154_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HPW09940.1"
                     /translation="MEPEAFLIDLDGVLYVGGSPVPGAIAAISYLRENGYSFRFVSNT
                     TRKSRATISRQLESMGFEIPVSHIFTPSMAAVSYITGKGGTEAAILTTADVSAEIIRD
                     GIQNREDSPRFVIVGDGGDLFGYSVLNKAFRLIIGGAELIALEKDRYWMGNDGLMLSA
                     GPFVAALEFATGTRAAVMGKPSREFFMRSLASMNADPARTVMIGDDIITDIGGAAAAG
                     LSGILVRTGKFRQEALAMATVIPKRIISSIGELPRLLSSGELCPDQ"
     gene            2845..3960
                     /locus_tag="PK154_02380"
     CDS             2845..3960
                     /locus_tag="PK154_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09941.1"
                     /translation="MTKKPLSMTFFAILAVFLLSSGCVSYLHSFFPTASYPEIEPQRG
                     ATIPAVPDYIYPFEDFYVTLSSDVDPEVYAGAQSAEKGVRIYDTSIGDDEWRSGLYRA
                     MTLDPAQGTFFDNLTGEFSQVRATYDLDSDEYLEVMTVFVQSLQYRNQNLSSPKYPVE
                     TYYDREGDCDDKSMLLASLLAHEGYNVSLLYFGPEQHMAVGVACQEEGYRDTGYAYIE
                     TTRVSFVGSEAGSLEGNITLVSDPLVIPIGSGTKGYTRCGEIRAINNELFNISARLEL
                     LATELRGRESSLLSRRSDIETMDRQLEMMLAEGDYFRYNRLVSEYNTRVGDYNQDLEA
                     YQKISDEYSRLAERYNYIISHEHDREGTYQFLFKGQW"
     gene            4115..4825
                     /locus_tag="PK154_02385"
     CDS             4115..4825
                     /locus_tag="PK154_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPW09942.1"
                     /translation="MIDLKTILLDLIKTRDLQKWVNHRYKNESRVILSKSTLTAISEF
                     KFDGDIRLPGNEWTYKNIDSIGQVLTDYHFTDLQPTDRVLDIGANIGIFSLLAAKKGC
                     KVTALEPLFHQELQYNLAYNNLKATVLPCALGSGSYIDIDFAGKKARVPIITMKKIKE
                     MYGPFNFLKCDCEGGEWTLQPEDLKGFRRLEMELHQCTHQVLPVNWSLVDWIEDNFVT
                     EIETLKKNRLWLLHAFNG"
     gene            complement(4882..5787)
                     /locus_tag="PK154_02390"
     CDS             complement(4882..5787)
                     /locus_tag="PK154_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW09943.1"
                     /translation="MPGKRREEKVTDKAGKSLIRGGSQRSNAMIPAEEPEGVHPFINR
                     IFCGDAAKILNRIPDNSVDIIVTSPPYNFGQRYAEDPHDDTHEWNAYFSELFVIWQEC
                     TRILKPGGRLAVNIQPLFSDYIPTHHIISSQLMRLGLLWKAEILWEKNNYNAKYTAWG
                     SWKSPSMPYLKYTWEFVEVFDKETHKKAGLKEDIDITDDEFKEWVIGRWSIPPETRMK
                     EYGHPAMFPEEIPRRLLKLFSYRNDIVLDPFNGAGTTTLVAWKQFRRFIGIDISPQYC
                     QKAIERIERSEVLAGTRRVPELEFL"
     gene            5889..6620
                     /locus_tag="PK154_02395"
     CDS             5889..6620
                     /locus_tag="PK154_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW09944.1"
                     /translation="MLEIQDLHVEIGGKEVLHDINLQIGEGETHVLMGPNGSGKTTLL
                     RAIMGFAGLSITEGRITFKGHDITHMPLHERAQLGMGMLFQRPPTIAGLKLGKLLEVT
                     SGGDLNRVTEYSKYLHMDKFLDRSINLGFSGGEIKRSEVLQLMIQKPDFVMLDEPESG
                     VDLENISLIGNAIAKLVDKDVHIVNRKKSGLVITHTGYILDYLDADFGHVMCDGVFRC
                     HGNPREILKVIKTSGYKECLACQKI"
     gene            6605..7720
                     /locus_tag="PK154_02400"
     CDS             6605..7720
                     /locus_tag="PK154_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPW09945.1"
                     /translation="MPEDIEQMEDLSSTEKERLAQTGLEVDMKNRSGSFFQVDHDIRQ
                     VASLEKGIEVLAIGEALKKYDWLQDYYWKLVPKDKDQYTRFVADQPEPRGFVIIARKG
                     SKTLYPLQACLFLSKTPVQHVHNLIIAEEGAELHIISGCASASYTKTGSHVGVSEFYV
                     GKGARVTSTMIHNWGEEISVFPRSAALVEEDGVFLSNYVCMQPVRKIQMAPVAYLKGR
                     NAIGRFSSITVTTPGSYMDLGSVVHLSAEGTGAELLTRAITTGGTLISRGLIEGEVGH
                     TKGHIECKGLILKDGIIHAIPEIKGNVVDTELSHEAAVGKIARDEIEYLMSRGLDESV
                     ATSTIIRGFLDVKIEGLPEVLQKQIDSAIDAAEKSGF"
     gene            7736..8386
                     /locus_tag="PK154_02405"
     CDS             7736..8386
                     /locus_tag="PK154_02405"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPW09946.1"
                     /translation="MTGTDPWESARERMVATQIAARGVGDEKVLAAMRRVPRHLFVPA
                     EVRGSAYSDYPLPIGHGQTISQPYIVAMMTSLLQIQPDDRLLEIGSGSGYQAAVLGIL
                     AREVISIERIPEVAQLAKKNLDDAGITNVTVVIGDGTLGFPGGAPYNGVLITAATPSI
                     PSPLVEQLAEGGRIVAPVGSRDLQELVRLTRKGHDLTRESFGGVVFVPLLGEYGWK"
     gene            8377..9042
                     /locus_tag="PK154_02410"
     CDS             8377..9042
                     /locus_tag="PK154_02410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPW09947.1"
                     /translation="MEVVVKNGRIYDITRPLAPGCIVYPGDIVPGFAQEDHGNYLITE
                     IRISSHSGTHIDAPSHYLKQGAGVDEIPLTHLMGWCRVIDVTHIQGEIKKSDLEGKSS
                     GAKKVLFKTSFSDQNEFVEDYPSLGIEAAEFLAAQRIHCVGTDAPSIEQYNCSGSVHR
                     TLLSAGTVVIEMLDLWRIPEGDYWMIALPLRLSGLDGSPCRALLFEGWEEEHPSWTLY
                     EVQ"
     gene            9018..9623
                     /locus_tag="PK154_02415"
     CDS             9018..9623
                     /locus_tag="PK154_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09948.1"
                     /translation="MDLIRSAIKRLQTRIEGSGSEEGVVRIDIDPDRVVCQYEKGACL
                     KAAYGGRSSEFVTMDPLTATTRIGFMFGAKLEQSWQRAAACAILNVVAGFFCISRVLK
                     SCPPESHRECLGALRCEIGDAPLYPVGMMAAAKEKFSHLVTDPHAAEIILVVGDGLIH
                     PEIGDLIEKYREEKRILFLSPSTVGVSALLGCEHWCPFGKI"
     gene            10058..12655
                     /locus_tag="PK154_02420"
     CDS             10058..12655
                     /locus_tag="PK154_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HPW09949.1"
                     /translation="MADIGLFRYRNIGIIVILIILAAFSLWIRVLPLFGPHAADILSI
                     VGSDDPLYNLRQIEQILANFPGYSWFEAMTLFPTGQTVPWGPLFTFICSVVCLVAGAT
                     TRTEIIQAALWVPPILAALMVPVIFVLVRKIWDWKAGLIAAALIAVIGGQFFFRSLAG
                     YLDHHIAEVFFSTIYCIAYLYCLDFCRKSPVDLKVWETLKIPVLVSCIAGVAYVLGFL
                     TMPTMILFAFITALTTICLFIYEYYHGRHGEYLVLINSVVFGIALFVSLFFLPSSGFA
                     LYYYSLGHPLSYLLLIVGTIVLYAISRFLKGKPWYSYPAIVIGLIVVGILVMAPALPT
                     LYQTFIDGLMAFFGLSPYTVTIQEARPWTLGEAWAVFNFGLLLMIGGIFALLYKNWKE
                     YQPGHIFVLVWSVFIIIAAFREVRYEYYLAVNIALLGGICAGFALEHAWPDILAMGKK
                     AAVKEPEPEQKEVKGSTKKKKADRISQKSTRKAVSKSKINYVNILFAGLACAFALIFA
                     VLSLQQQYAVASSEGIRMNQDWRESLEWMNGNTPETGVDYYTIYEEDNFTYPATAYGV
                     MSWWDYGHMITYLANRIPNSNPFQAGVAGPNGSATFFVSGMEAATNQVADNQGTRYVM
                     TDIEMATGKFWAMATWYNATEKQAPYQPTYYIPDPANPGTYQPLTAYKDLYFLTTVSR
                     LHNFDGSYTPAGEVYYIEYTTSIGSRPDQAVITKATVMDADEANAAADRYNQQAQPGT
                     GAAVVSTNFIQPTTDIPALSHYRLVHESPTNVFSGSSPDVKYVKIFEYVPGARIKGDG
                     IIELPLVSNTGRAFVYRQQSTNGEFVVPYSTEGNPYDVRATGKYKITGTGRQISVSED
                     AIMRGLQIT"
     gene            12659..13636
                     /locus_tag="PK154_02425"
     CDS             12659..13636
                     /locus_tag="PK154_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPW09950.1"
                     /translation="MTSQCSAICGEEFTAHNMAISRENRARIENVITRLPDYVRRHPP
                     ESASSVDLERVHDVRHIRMIEEFSSYGGLHYIDMDTYVTENSFTTALFAAGSSIRACS
                     RALDGEHCFAIIRPPGHHAEPDRAMGFCLFNNAAVAAAWALETVDRVAIIDWDLHHGN
                     GTQKIFYRSDRVLFCSIHQSNAFPGTGWIDEIGEGRGRGFTLNAPLLPGSTIDDYACV
                     FEEVFAEAISHFSPNLLIISAGQDPLSDDPMEGMRLVPRDFGVLTRILMEAHENPLAL
                     VLEGGYGPSLGEAVAATFDALHGEPVKMPYGDTRQSTRKVVAHLKKVMI"
     gene            complement(13744..>14640)
                     /locus_tag="PK154_02430"
     CDS             complement(13744..>14640)
                     /locus_tag="PK154_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPW09951.1"
                     /translation="EYRMDRMEYAAYEELPVHRTVNPADLILPRDLHEREKLHWSPAL
                     DLLSGDEVYMPSNAVFHPYDTLGMTFPLFQSDTNGLASGNVREEAILHGILEVMERDD
                     MSVASRTRCMGRRLELDHPSPARDIRNKMESSGIEIHLWLLSGRTRIPTVAAAADDTE
                     TKDPELLVMGSGTHPDPSIAALRALTEVAQSRASHLSGGRENPGRKSLVERAGYDRMK
                     RINREWFAPAEEFPISSMDDLSTPYIDDDIRLVLEELRSFAERVCVCDLTRTVIPVVR
                     VIIPGFEVSHVNPARIRSHSTR"
BASE COUNT         3467 a         3768 c         3909 g         3496 t
ORIGIN      
        1 agaatgaact ccactggtgg tgccatggca gtatctggtg cagttcatgc ggattagtca
       61 ggataatgcg tgcagatctt ctgatacgcg gtcgcttgtc tgccggtgta tccccatcat
      121 aaagggctgg tgaaaccctt atcccggtac ctttctccag ctcctgcaca gctttcagct
      181 gatcattggc aagtgccttt gtcgggtaca gaaacagtgc tgtcgattcg ggatcccgac
      241 ccagggtttc aaacacaggg aggaggaagg ctagggtctt tcctgatgcg gtggaagtgg
      301 caaggacgat gttctcacca ctcatggccg catccatggc tgcagcctgg tggcggtaaa
      361 gccggatacc cctcgtgttg agataggtac tgatgcggga ggagagagaa caggtgccgt
      421 attctccatc gctccggtaa aattctttca cggtgaagat atgccttgaa aaagaggggt
      481 cttctgaaag ggcatgcagg agggagctca cggtcacgat acacactccc cctgcaggaa
      541 acagaagagc cgggcaagcg aaacaagatc ctgcctgttg tgggtcacga ttgggacaag
      601 ggggccggga ttccctgttg tcaggaacgc ttcgtaaaat tccggcacca tcgcccccgg
      661 gatatcatcc tctcttccgg tgccaagtat ccgctgttca agagtcacca gccggcagtc
      721 gggaagttca cctctccagc gcctccgcga gaaatggagc agatcgtaat gaggatttcc
      781 tatcacggcc ggcctgccgt aaaatgcagc acgtcctgca aggtacggga tatcaaatgc
      841 tttcccgtta tagctgacca gaacccggtc ctcctccagc tgatccatga caactgaaag
      901 ggccgggatc tcttcgttga tgtccctcag cagatactgg gagatgaaca gcgtaccgga
      961 acggacctct gcaagcccga aaagtatgat aggccgggag aagaggccga gggtctcgat
     1021 gtcgatgaag atcagatcgc ggggatggta gaacccggca gtgagaaggc atgagggatg
     1081 ggagggtgag agccaccggg agatgagttg ttcgattggc tgaatctcat gtcctgaaag
     1141 gattgccatg gtttcacggg cagctttccc atatcttcgg tgaacggcaa gatcgacaat
     1201 cgtggcatat cccctggcct tcaactccct ctcttttctc ctcccgatac catagacaag
     1261 cgtgaggtcc gagagaagag tggaccggat tgcggcttta ttgatcccct tcagatccag
     1321 tggctcgcgg gaagggatgc agtaacaggt cccttcgtag gtgtcacatt cccgaccatg
     1381 aaaaatctca tcgagcgatt tatcgccata ggttgccata gcggcatgca attgtgctgt
     1441 tgcctgcgta tgcagggact caaagacata cctgtcatgg aaaccggttc ggaatatgga
     1501 atcattccgg ataacagcat aatcatggga ttccccgatc cgggatcgcc attgcctgct
     1561 tatcgaggga taaacatgac ctgattccat tcgcgctatg ctgctcttgg tcctctctcc
     1621 ctttaatccg gggcgtgcac ggtgaaagtg accaggggtt cgaaaatccc atatattccc
     1681 gatatctatt tgtaagtatc tcgataccag aaccggcctg attgccggga tgagtagcag
     1741 taaaggcgag acatttgatc gacatcttca ttcatggcaa ggagaattcc ccatggatgt
     1801 tccagcgtct tcttgatcag gctgggtatt acaactgttc actctataaa acaggaaaag
     1861 tatgctgcag ggaactcatc tctggtggtt tggagagtgt cagtaatgaa gaggaatgtc
     1921 cctggttcag caatgaaaaa aagcgtgtca ggagctaccg tgtggctgca atctgaggca
     1981 acatcctcgc ttgatgaacc ttggcggatc tgtaacgtgc tctatgaaac ctttttgaac
     2041 gggagttatc ggaatggaac ctgaagcctt cctgattgat ctcgacggag tcctctatgt
     2101 tgggggatca ccggttccgg gtgccatcgc tgcgatatca tacctgcggg agaacggcta
     2161 ttccttccgg ttcgtatcca acacgacaag gaagtcccgg gcaaccatct cccggcagct
     2221 ggaatccatg ggatttgaaa tccccgtctc ccatatcttt actccttcca tggcagcggt
     2281 gtcgtatatc acaggaaaag gcggaaccga agctgcaatc cttaccactg ccgatgtttc
     2341 tgcagaaata atccgtgacg gcatacaaaa cagggaggat tctcccaggt tcgtcattgt
     2401 tggcgacggt ggagatctct tcggctattc cgtcctgaac aaagcgttcc gccttatcat
     2461 tggaggagcg gaacttatcg cgctggaaaa ggacaggtac tggatgggga atgatgggct
     2521 tatgctctcc gcaggaccat ttgtcgcagc acttgagttc gccactggaa ccagggcggc
     2581 ggtgatggga aaaccctccc gcgaattttt catgcgttcc cttgcctcca tgaatgcgga
     2641 ccccgcccgt actgtcatga ttggggatga tattatcacc gatattggag gagcggcggc
     2701 tgccggcctt tcaggaatcc ttgtcaggac cggtaagttc cggcaggaag cccttgcaat
     2761 ggccacggtg atcccgaaaa ggatcatatc ctcgattggc gaactgccgc gcctcctctc
     2821 atcaggggag ctctgtccgg atcaatgacg aaaaagcctc tttccatgac gttttttgcc
     2881 atactggccg tattcctcct ctcttccggt tgtgtaagtt acctccacag tttttttccc
     2941 acagcatctt accctgaaat cgaaccacag cggggggcga cgatccccgc tgtccctgac
     3001 tatatctatc catttgagga tttttatgtc accctctctt cagacgtcga cccggaagtc
     3061 tatgcagggg cccaatcggc cgagaaaggg gtgaggatat atgatacttc gattggcgat
     3121 gatgaatggc gatcaggcct ctacagggct atgaccctgg accctgctca gggtacgttc
     3181 tttgacaacc tgaccgggga gttcagtcag gtcagggcca cgtatgatct cgactcagat
     3241 gagtatctcg aagtgatgac ggtgttcgtc cagtccctcc aatacaggaa ccagaatctc
     3301 tcgagcccaa aatatcccgt cgagacgtat tatgaccggg aaggggattg cgatgataag
     3361 agcatgctgc ttgccagcct ccttgcacat gaaggctata atgtctccct cctttatttc
     3421 gggcccgaac agcatatggc ggtgggggtg gcctgtcagg aagagggata ccgtgatacc
     3481 ggttacgctt atatcgagac cacgagagtg agttttgtcg ggagcgaggc tggatcgctt
     3541 gaagggaaca ttacactcgt atcggatccg ctcgtcatcc ccatagggag tgggacgaaa
     3601 ggatatactc gttgtggtga gatacgtgcg atcaataacg agctgttcaa catcagcgca
     3661 cgcctggaat tactcgcgac agagctccgt ggtcgggagt cctctctcct ttcccgccgt
     3721 tcagacattg agaccatgga ccgccagctt gaaatgatgc tggcagaagg agattatttc
     3781 agatataacc ggctggtatc ggaatacaat accagggtcg gtgattataa ccaggatctt
     3841 gaagcatatc agaaaatctc tgatgaatac tcccgtcttg ccgaacggta caattacatc
     3901 atctcgcatg aacatgaccg ggaaggaacc tatcagtttt tattcaaggg acaatggtaa
     3961 acatagatcc gaaagggtag aattttagta atggggtgga ccgctacaat agctcaatat
     4021 tttagattgg aatgatcaaa attcatcttt tatggatact aatctccata tgcgataagt
     4081 ttaaccatat gaaagcgcgg agcactttaa tagcatgatt gacctgaaaa ccattttact
     4141 cgatctgatt aagacaagag atcttcaaaa atgggttaat cataggtata aaaatgaatc
     4201 aagagtcatt ttgagcaaat ctacattaac tgccatcagt gaattcaaat tcgatggtga
     4261 tatccgatta cctggaaacg aatggaccta taaaaatata gattcgattg gtcaggtgtt
     4321 gactgattat cattttacag atcttcaacc aacagaccga gtcctagata ttggagctaa
     4381 tattggtata ttttcccttc ttgcagcgaa gaaaggttgt aaagtgactg ctttggaacc
     4441 actatttcac caggaactac aatataatct agcctataat aatttgaaag ccaccgtttt
     4501 accatgcgca cttggatcgg ggagttatat cgatattgac ttcgcaggaa aaaaagccag
     4561 agttccgata attaccatga aaaagattaa ggaaatgtac ggtcccttca atttcctaaa
     4621 atgtgattgt gaaggtggtg aatggaccct tcagccggaa gatctcaaag gattcagaag
     4681 gttagagatg gaattgcatc aatgtactca tcaggtcctg cccgtcaact ggtctctagt
     4741 ggactggata gaggataatt tcgtaacaga aattgaaaca ttgaagaaga atcgcttgtg
     4801 gctgctgcat gccttcaatg gataattgaa aaaaagcatg cctaccatgc ttacattgcg
     4861 cttcttgatt cgcggcactc ctcataaaaa ttcgagttcc ggaactctcc tcgttccggc
     4921 taagacctcg gatcgttcga tccgttcaat tgctttctgg cagtactgag gggatatatc
     4981 aatcccgata aatctccgga actgtttcca ggcgaccagg gtcgtcgtcc ctgcaccgtt
     5041 gaagggatca aggacgatgt cattccggta cgagaacagc ttcagtaacc tccgggggat
     5101 ctcttcagga aacatggcgg ggtgaccgta ttccttcata cgagtctccg gcgggatact
     5161 ccatctccct atcacccact ccttgaactc atcatcggtg atatcgatat cctccttcag
     5221 acctgccttt ttgtgggtct ccttgtcaaa gacctccacg aactcccagg tatacttgag
     5281 atagggcatg gagggagatt tccagctccc ccaggccgtg tattttgcat tgtaattgtt
     5341 cttctcccag aggatctcgg ccttccagag gaggcccagt ctcatgagct gcgaagagat
     5401 gatgtggtgg gtcgggatgt agtctgagaa gagaggctgg atgttcacag cgagcctccc
     5461 tcccggttta aggatccgtg tgcattcctg ccagatcaca aacagttctg aaaaataggc
     5521 attccactca tgggtgtcat catggggatc ctcggcatat ctctgcccga agttgtaggg
     5581 gggtgatgta acaatgatat ccacgctgtt gtccgggatc ctgttcagga tcttcgcggc
     5641 atcgccacag aatatccggt tgatgaacgg gtgcactccc tctggttcct ctgcgggtat
     5701 cattgcattc gaacgctggc ttccccccct gatgagagat tttccggcct tgtccgtgac
     5761 cttctcctcc cgcctctttc ctggcatctg attctaatgg gggctgtcga gcctttattg
     5821 tgccgggcga tatggcaggg tgaaaaccgt ttaatcccgg ggtgcatata cactaaagac
     5881 gatcgcagat gctggaaata caggatctcc atgtggagat agggggcaag gaggtgcttc
     5941 atgatatcaa cctccagatt ggggagggtg agacgcatgt cctgatgggc ccgaacgggt
     6001 ccggaaagac aacccttctt cgggctatca tgggttttgc cgggctgagc ataaccgaag
     6061 gtcggatcac cttcaaagga catgatatca cccatatgcc ccttcatgaa cgggcacagc
     6121 tggggatggg gatgctcttc cagagacccc ctaccatcgc cggtctcaag cttggcaagt
     6181 tgctcgaggt tacttcagga ggagatctga accgggtaac cgagtactcc aagtacctgc
     6241 acatggataa gttcctcgac cggtcgatca acctcggatt ctccgggggt gagatcaaaa
     6301 ggagcgaagt cctccagctg atgatccaga aaccggattt tgtaatgctc gatgaaccgg
     6361 agagcggggt tgacctcgag aatatatccc ttatcggcaa cgctatcgcc aagctggtcg
     6421 acaaggatgt ccatatcgtt aaccggaaga agagcgggct tgtgatcacc cataccggct
     6481 atatcctcga ctatctggat gcagacttcg ggcacgtcat gtgcgacgga gtcttcaggt
     6541 gtcatggaaa tcctcgggag atactcaagg tgatcaagac atcgggttac aaggagtgcc
     6601 ttgcatgcca gaagatatag agcagatgga ggacctctcc agcacagaaa aggagcggct
     6661 cgcacagaca ggtctcgaag tggatatgaa gaaccgctca ggcagcttct tccaggtcga
     6721 tcatgatata cggcaggttg cctcgctgga aaagggtatc gaggtccttg ccattggtga
     6781 ggcactcaaa aagtatgact ggctgcagga ctattactgg aaactggtgc caaaggacaa
     6841 ggatcagtac accagattcg ttgccgatca gcctgaaccc cggggatttg tgatcattgc
     6901 ccggaagggg agcaagacgt tatatcccct gcaggcctgc ctcttccttt caaagacccc
     6961 ggtccagcat gtccacaacc tcattatcgc agaagaaggt gcagagctcc atatcatatc
     7021 agggtgtgca agtgcttcct acacaaagac gggatctcat gtgggggtga gcgagttcta
     7081 tgtcgggaag ggagccaggg tgacttcaac catgatccac aactggggtg aagagatcag
     7141 tgtcttccca cggagtgcag cccttgtcga agaggacggg gtcttcctct ccaattatgt
     7201 ctgcatgcag ccggtgcgaa agatccagat ggctcctgtg gcgtacctca aagggagaaa
     7261 tgcaataggc cggttctcaa gcatcaccgt caccacccct ggatcgtaca tggacctggg
     7321 atcagtggtt cacctctcag ccgaaggaac aggtgccgag ctgctcaccc gagcaatcac
     7381 cactggcggc acgctcatct cacggggatt gattgaaggg gaggtggggc acacgaaagg
     7441 ccatatcgag tgcaaaggcc tcatcctcaa ggacgggatt atccatgcca ttcccgagat
     7501 taaagggaat gttgtggata ccgagctctc tcatgaggct gcggtgggga agatcgcccg
     7561 tgatgagatt gagtacctca tgtcgcgggg gctggatgag agcgtggcga cctctaccat
     7621 aatcaggggg ttccttgacg tgaagattga aggcctcccc gaggtcctgc agaaacagat
     7681 agactccgcg atcgatgctg cggagaagag cggattctaa aaaagagagg gcgggatgac
     7741 aggcaccgac ccctgggagt ctgctcggga acggatggtc gcaacccaga ttgcggcacg
     7801 cggagtcggc gatgaaaagg tcctggcagc gatgcgccgg gtcccccgcc atctttttgt
     7861 acctgcggaa gtacgcgggt ccgcatacag cgactatcct ctccccatcg gacacggcca
     7921 gaccatctcc cagccctata tcgttgcgat gatgacctcc ctgctccaga tccagccgga
     7981 tgaccgttta cttgagattg gatctgggag cgggtatcag gcagcggtcc ttggaatcct
     8041 ggcgcgggag gtcatctcga tcgaacgtat ccctgaggtg gcgcagcttg cgaagaagaa
     8101 tcttgacgat gctggaatca ccaatgtcac ggttgtcatt ggtgatggca ccctgggatt
     8161 tccaggcggt gccccatata acggtgtgct gatcacggcc gcaaccccct caattccgtc
     8221 gccgctggtt gaacagctcg cggaaggggg gagaatcgtt gcccctgtgg gatcaaggga
     8281 cctccaggag ctggtacggc tgacgaggaa aggtcatgac ctgacccgcg agtcctttgg
     8341 cggggtggtc tttgtgcccc tgctcgggga gtatgggtgg aagtagtggt taaaaacggg
     8401 aggatctacg atatcacccg gccgcttgct cccggatgca tcgtgtatcc gggagacatc
     8461 gtcccgggat ttgcccagga agatcacggg aattacctga tcaccgagat acgaatcagc
     8521 agtcattccg gtacccatat cgacgctcca tcccactatc tcaagcaggg tgcaggagtc
     8581 gatgagattc ctcttaccca cctgatgggc tggtgccggg taatcgacgt cacccatatc
     8641 cagggtgaga taaaaaaaag cgatcttgaa gggaagagta gtggtgcgaa aaaagttctt
     8701 ttcaagacct cattttcaga ccagaacgaa tttgtcgagg actatccgtc acttgggatt
     8761 gaggctgcgg aattccttgc agctcagcgt attcattgcg tgggaaccga tgccccgtcc
     8821 attgaacaat acaactgcag cggatcagtt cacaggacac tcctctcggc agggacggtg
     8881 gttatcgaaa tgctcgatct ctggagaatt ccggaaggag actactggat gattgccctt
     8941 ccgctgcggt tgagcgggct tgatggatct ccctgccgag ctctcctgtt cgaggggtgg
     9001 gaggaggaac acccatcatg gaccttatac gaagtgcaat aaagagactg caaacgagga
     9061 tcgaaggatc gggatcagag gaaggagtag tcaggattga tattgatcct gacagggttg
     9121 tctgccagta tgagaagggt gcctgcctga aagcagcgta cggcggccgg tcctccgagt
     9181 ttgttactat ggatccattg acggcaacaa cccggatcgg attcatgttc ggtgcaaagc
     9241 tggaacaatc gtggcaacgg gctgcggcat gcgctatcct gaacgtagtt gcaggatttt
     9301 tctgtatttc ccgtgtcctc aagtcctgcc cgcccgaatc tcatcgtgag tgcctcggtg
     9361 cgctcaggtg cgagatcggg gatgccccgt tatacccggt tgggatgatg gcggcagcaa
     9421 aggaaaaatt ctcccatctg gtgacggacc cccatgctgc agagataatt ctggtcgttg
     9481 gtgatggact gatccaccct gagatcggcg atcttattga aaaatatcgg gaagaaaaaa
     9541 ggattctctt cctctccccc tccacagtcg gggtaagtgc cctgctggga tgcgagcact
     9601 ggtgcccgtt tgggaaaatc tgatccttct tttcctggta ttcaccacct gttccactct
     9661 gccggacctg tgaagggacc tcttccgaat cagaagccat taccaatgga agagaatgct
     9721 ttgttcttgt gaggatacct ggtagcggag ggtcccgtcg tattaatcat ggcaggatct
     9781 gatcccttca aggtcccaca gctattccgt gtgtctggct tcgcagccct ttataatagg
     9841 gtataacgat ttcaaaatag ggtgagtagt ggtaatcgca atacagactg catggcgtgt
     9901 cccacgggaa tcgggtatac tctcgattgt ggtttaagtt aaaataaatg tattttgggt
     9961 tttgtttcga aatttatacc atattattgg atggttcaca aactattaac ataatggaac
    10021 attaacatgt gctgagtaca caatagggag gtcaacaatg gcagatatcg gtttatttcg
    10081 atacaggaac atagggatta ttgtcatctt aataatcctt gcggcatttt ccctctggat
    10141 acgggtgctc cccttgttcg ggccccatgc cgccgacatt ttgagtattg tgggaagtga
    10201 tgatcccctc tataaccttc ggcagatcga acagatcctc gctaatttcc ctggctacag
    10261 ctggtttgag gcaatgacac tctttcccac aggccagact gtgccatggg ggccgttatt
    10321 cacctttatc tgcagtgttg tatgcctggt tgcgggagca acaacccgga cggagatcat
    10381 ccaggctgcc ctctgggtac cacctatcct tgcggcattg atggtaccgg tcatctttgt
    10441 ccttgtcagg aaaatctggg actggaaagc cggcctgatc gctgcagccc tgatcgcggt
    10501 tatcggtgga caattcttct tccgttccct ggccgggtac ctggaccacc atatcgcaga
    10561 ggtattcttc agcaccatct actgcatagc ctacctctat tgcctcgact tctgccggaa
    10621 aagccccgtc gacctgaaag tatgggaaac gctgaaaata ccggtcctgg tctcctgcat
    10681 tgccggcgtt gcctacgtcc tcggcttcct caccatgccc accatgatcc tctttgcctt
    10741 catcactgcc ctcaccacca tttgtctctt catttacgaa tattaccatg ggagacacgg
    10801 ggaatacctc gttcttatca atagtgtggt gttcggcatt gcgctgttcg tctccctctt
    10861 tttcctgccc tccagtggat ttgcgctcta ctattactct ctcggacacc ccctctccta
    10921 cctgctcctg attgtcggga ccatcgtcct ctatgccatc tcccgattcc tgaaaggaaa
    10981 gccttggtac agctatccag ccatcgtgat tgggctcatc gtggtcggca ttcttgtcat
    11041 ggcgcctgct ctcccaacct tatatcagac gttcattgat gggctcatgg ctttcttcgg
    11101 ccttagcccc tataccgtca ccatccagga agcgcgacca tggacccttg gtgaggcgtg
    11161 ggcggtcttc aacttcggcc tcctgctgat gattggaggg attttcgccc ttctttataa
    11221 gaactggaaa gaataccagc ccggacatat tttcgtcctc gtctggtcgg tctttatcat
    11281 tatcgcagca ttccgggaag tccggtacga gtattatctg gcggtgaaca tcgcactact
    11341 cggcggaata tgcgctggat tcgcccttga acacgcctgg ccggatatcc tggccatggg
    11401 aaaaaaggcg gccgtaaaag aaccggaacc agaacagaag gaagtgaagg gatcaacgaa
    11461 aaagaagaaa gctgatagga tatcccagaa atcaaccagg aaagcagttt caaagtctaa
    11521 gatcaactac gtcaatatcc tctttgccgg acttgcgtgt gcctttgctc tcatcttcgc
    11581 ggtgctatcg ttacagcaac agtatgccgt ggcatcttct gaggggatac ggatgaacca
    11641 ggactggcgg gaatccctgg aatggatgaa cggcaatact cctgaaaccg gagtggatta
    11701 ttataccata tatgaagaag acaacttcac ctatccggca acagcatatg gtgtcatgtc
    11761 gtggtgggac tacggccata tgatcaccta tcttgccaac cggattccga actcgaatcc
    11821 gttccaggca ggagttgctg gcccgaacgg ttctgcgaca ttcttcgtat cagggatgga
    11881 agcggccacc aaccaggttg cggacaatca gggtacacgg tatgtgatga ccgatatcga
    11941 gatggcgaca ggcaagttct gggcgatggc gacctggtac aatgcgacgg aaaagcaggc
    12001 cccctatcaa cctacctact atatccccga tcccgccaat ccaggaacct atcagccact
    12061 cactgcatac aaggacctgt atttcctgac cacggtatcg agattacaca acttcgatgg
    12121 ctcctacact cccgctggag aagtctatta cattgagtat accacctcaa tcggcagcag
    12181 gccggatcag gcggtcatta ccaaggcgac cgtgatggat gccgatgagg caaatgcagc
    12241 cgcagaccgg tataaccagc aggcgcaacc cgggactggt gctgctgtag taagtacgaa
    12301 tttcatccag ccgacaaccg atatcccagc gctcagccat taccggcttg tccatgaatc
    12361 gccgaccaat gtgttttctg gttcttctcc cgatgtgaag tacgtaaaga tatttgagta
    12421 tgtcccaggg gcccggatca agggagacgg gatcatcgaa ctgcctctgg tttccaacac
    12481 cgggagagcg tttgtgtacc gccagcagag tacgaatggg gagtttgtcg taccctattc
    12541 taccgagggg aatccctatg atgtacgggc gactggcaag tacaagatta ccggaaccgg
    12601 aaggcagatc tcggtttcag aagacgcgat catgagaggc cttcagatca cctgaacgat
    12661 gacatcccag tgctcggcca tctgcgggga ggaattcact gcccataata tggcgatctc
    12721 ccgggagaac cgggcacgta tcgagaatgt catcacccgg cttcctgatt atgtcaggcg
    12781 gcatccccct gaatctgcca gttcagtgga tctcgaacgg gtccatgacg tccgccatat
    12841 ccggatgatc gaggagttca gctcatatgg cggtctccac tatatcgaca tggacaccta
    12901 tgtgacggag aattccttca ctaccgctct ctttgctgcc ggatcgtcca tcagggcatg
    12961 cagcagagca cttgatggcg aacattgttt tgccatcatc cgccctcccg ggcatcatgc
    13021 cgaacctgac cgggccatgg gattctgcct cttcaacaat gcggcagttg ccgctgcatg
    13081 ggcgctggaa acggttgatc gtgtcgcgat catcgattgg gatctgcacc atgggaacgg
    13141 tacacagaag atcttttacc ggagcgatcg ggttctcttc tgctccatcc accagagtaa
    13201 tgccttcccg ggaacagggt ggatcgatga gatcggtgag gggaggggtc ggggattcac
    13261 cctcaacgcc ccccttttac ctggcagcac catcgatgac tatgcatgcg tgttcgagga
    13321 ggtgttcgct gaagccatct cgcacttctc acccaatctc ctgatcatct ctgcgggcca
    13381 ggaccccctc tcagacgatc ccatggaagg gatgcggctc gtcccgcggg attttggcgt
    13441 cctgacccgt atcctcatgg aagcccacga gaatccgctc gctctcgtcc tggaaggggg
    13501 atacggcccc tcactcggtg aggcggtcgc cgccacattc gatgcattgc atggagaacc
    13561 ggttaaaatg ccctatgggg atacccgtca gagcaccaga aaagttgtcg cccacttgaa
    13621 aaaagtgatg atatgagata gcaagatcct ggttgggacg cgtgagcact caattatcct
    13681 ccgattttcg tcacctgcct cgttcttcca ggcagggaat ttttgagtaa ggtgcaggga
    13741 aggctatcga gtggaatggc tccggatacg cgcaggattg acgtgtgaaa cctcaaatcc
    13801 tggtatgatg accctgacaa ccgggatgac agtccgtgtg agatcacaga cacaaaccct
    13861 ctcagcgaag gatcgcagtt cctcaagcac cagccggatg tcatcatcga tatagggagt
    13921 gctcagatcg tccatggaag agatcgggaa ctcttctgca ggggcaaacc actccctgtt
    13981 aatccgtttc atacggtcat accctgcccg ctcaaccagg cttttccggc cgggattctc
    14041 tctgccgcca gacaagtgcg aagcccggct ctgggctacc tcggtaaggg cccggagtgc
    14101 tgcgatagaa ggatcgggat gcgtcccaga acccatcacg agcagttccg ggtctttggt
    14161 ttccgtgtca tctgctgctg ccgcaacggt aggaatccgg gttctcccgg aaagcaacca
    14221 gaggtggatc tcgatcccgc ttgactccat cttattcctg atatcccgtg ccggactggg
    14281 gtggtcaagc tcaagccgcc tccccatgca gcgggttctg ctggcaacac tcatatcatc
    14341 ccgttccatc acctcgagaa taccatgcag gattgcctct tccctcacgt tcccgcttgc
    14401 aagaccgttg gtgtcgctct ggaacaacgg aaaggtcata cccagcgtat catacggatg
    14461 gaagaccgcg ttgcttggca tatacacctc atcacctgag agcaggtcca gtgcaggact
    14521 ccagtgaagt ttctcccgtt catgcagatc ccgtggcagg ataagatctg ccggattgac
    14581 cgtcctgtgt acgggcagtt cttcgtatgc tgcatactcc atccggtcca tccggtattc
//
LOCUS       DAOOCO010000023        14557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3769_length_14557_cov_5.435871, whole genome shotgun sequence.
ACCESSION   DAOOCO010000023 DAOOCO010000000
VERSION     DAOOCO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_14557_cov_5.435871"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1848
                     /locus_tag="PK154_02435"
     CDS             343..1848
                     /locus_tag="PK154_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW09952.1"
                     /translation="MNNPLLRYAFLFIAACFVAGMVFLTGCTSAPSKTGKLDDFDEFV
                     TVKMAEYEVPGAVVGIVENDTVVYLKGFGVREIGRPEKVDPDTRFQIASVSKYVTAGA
                     IGTLVEEGKLGWDTPVVTYLPGFALKDTYAGGHATLRDLLAHRTGFKSYDGELLGRVG
                     YSNSEILERIEYLEPGGSFREKYQYSNIGYFIAGEVAAAADNQSWEDLTDTRILRPLN
                     MTRSGAHPETLYLDDNHVSGHGGAPGDVHIIPLEEASLPAAGQVVSTGRDMTQWLRMM
                     LNEGSVDGKQILTPKTVKEIHAASMVAGASGPLRDPNGATGLGCDSYHFLGERVIEKN
                     GALDGVRSIVILIPGKKSGIVVIANKQLTAFPEAVRDEFLERYIGKAGIDLQAREKAS
                     QAGWNSLIKNPERPAAAGPATIISSAMAGNYRSELYGTLEVNLGSDPGNMTIGLGPEK
                     YPGNLTHWTNNTWYLSFPNPDDQVGYFTFTENTSGSVTGFTSEEFGRFTRV"
     gene            complement(2074..2679)
                     /locus_tag="PK154_02440"
     CDS             complement(2074..2679)
                     /locus_tag="PK154_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPW09953.1"
                     /translation="MIVRISDKIRGFLFSPSEAFWQVRDEHLQDTMIYFVIISIIYTL
                     LSTIISGIAVSKHPALVLAGLGFGSGNVVLSFFIILALTVVMAVVYACWLHLWIIMVC
                     GRNGLEKTIKATLYSLTPAILIGWIPVIGAFAAPLWSVVVGVIGIQELHKASFGKAMI
                     AILIAIITAAILAAVLFKPLLAEVIASGPDLIGPYHSRPIL"
     gene            complement(2764..3426)
                     /locus_tag="PK154_02445"
     CDS             complement(2764..3426)
                     /locus_tag="PK154_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HPW09954.1"
                     /translation="MRHGIVIFLGPSLDPAAAGRLLAADYRPPAKRGDITDAAAEGAR
                     IITLIDGVFFQDCSVGHREILGALAQGVRVIGASSMGALRAAELAPLGMEGVGTIFRL
                     YRDGILTSDDEVALVYDPETNLPLSEPLINIRCTIRAAEREGVLSPASAGLLLSIAQS
                     LYFPERTWEAICQGAGERIASQERERFAGFLSTSAVDQKKADALEAIARTREIAAERG
                     LL"
     gene            complement(3386..4600)
                     /locus_tag="PK154_02450"
     CDS             complement(3386..4600)
                     /locus_tag="PK154_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HPW09955.1"
                     /translation="MLLSSCRKVYMNDTQRTVSPEETLARVERVLPAAGITRVADITN
                     LDRIGIPVFSSIRPTAGLGAVSVYNGKGATPAEAKVSAMMEGIERYSAELDSRTLLVS
                     PYSRLCGGEISVLDPARLILPAGSITDAPIPWVTGYDLGAGEEILVPASAVFHPLPRE
                     YPQLFRTSTNGLASGNTREEAVFHALMEIIERDAWSLAEVTRDTGPQVGEIDDPLAGS
                     LLEKFGAAGVEVTVKDITSDIGIPTIAAVSDDTLLQDPALLTIGMGTHTSARIAVLRA
                     LTEVAQSRLTQIHGAREDTTVADIRKKMGYERTKRMNRYWFSSHETRNFSDIPSFDTS
                     DFLEDIDRARERLKEAGLPQVIVVDLTRESIGVPVVRVIVPGLEVFAMDRERIGRRCH
                     EARDRHFSRSKP"
     gene            complement(4830..6131)
                     /locus_tag="PK154_02455"
     CDS             complement(4830..6131)
                     /locus_tag="PK154_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPW09956.1"
                     /translation="MEWLPVLITAVALYAAVALIIRQKGYFSEHIMFYGPIMAIKTMS
                     VGFFDRFRKYSTFLRAYATFGVLMVIVISVVMTILLVLSLSYTFAVRPEPTGIYAPQN
                     ILLIPGLNEYVPSTLAVWLAFVVTIAIHEFGHGILSRVENIRVRSMGALLFVLPIGFF
                     VEPDEEELSQARGMKKIRMFGAGITNNIVVGGLCFLALILLMGLAAPAAGPVIGGVYQ
                     NYSAEQAGIPAYSLIHAVNGTSVSTPADVAAILNSTRPGDTITLTIGHQGVISDYQLT
                     LSEWPEELANRTTGFMGVTYYDPALVLDTIKGSVSPIGFLRFLTVPFDIGGFGNPLRI
                     LALDTAAQEFYSVPWAGFFGIIHFLFWCGWINISVGLFNAIPMVPLDGGYILKEGVDR
                     LFERRGIGRYSLYVVTFISTLMIVILFSLIMLPYLFSLTAV"
     gene            complement(6204..6875)
                     /gene="rnhB"
                     /locus_tag="PK154_02460"
     CDS             complement(6204..6875)
                     /gene="rnhB"
                     /locus_tag="PK154_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW09957.1"
                     /translation="MFGGVDEAGKGAVLGPLVVACVGCESEEDLAAIPVRDSKTLSPA
                     ARTDLFGRIQESVHYTVLVITAEEIDIRRRSMTMNQLMAAAHAEVIRELHPAIAYVDA
                     CDVVAARFGRAVAARIGYPCRVIARHHADATIPVVSAAGIVAKVVRDRLIGDLAGEYG
                     VIGSGYPSDDTTIAFMEAYIGENGRPPPIARTSWETTRQILGRAGQTELSFFCSGSQE
                     SGQSP"
     gene            6934..7602
                     /locus_tag="PK154_02465"
     CDS             6934..7602
                     /locus_tag="PK154_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPW09958.1"
                     /translation="MYIIIVGLGGIGRSLAAQSVGNGDNVVIIDRNEQRCSEMLEHYD
                     VLAITGNATDKVILEDAGIDRADALVATTSDDSVNLMTCWLAKRFKVPNVVSIVNNVE
                     HSDLFKEVGVKISENPDELVASRLYYWARNPMLQQLASIPGGTIFEVVAEKGAPIVDH
                     EIRELKVKDFVFIAIRRLGGELIIPSGTIRIRPGDTITVFTKKEAEVETLELLNRQLR
                     VPAT"
     gene            complement(7822..9018)
                     /locus_tag="PK154_02470"
     CDS             complement(7822..9018)
                     /locus_tag="PK154_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW09959.1"
                     /translation="MRTEYDLLVIGGGPAGAIAARTAAQAGHSVLLVEKRPAIGAPVR
                     CAEGIGKEALAEFVKPDERWIAVELKRAEIVAPDGSSMMLEAAMAGSKIGYILDRKVF
                     DRELVWQAAEAGADVMVKTRASAPLMDGGRVKGAVLEYCGEQKKVEAAVTIAADGVES
                     KFSRWCGVDTAVPVREIMSSCQYLMTGIEIDHECTTFFLGNEVAPEGYLWIFPKGKRT
                     ANVGIGISGKKSGAGHRAKDYLDRFVRERFPNGKTIECIVGGVSVCRPLPRTVADGLI
                     IAGDAARVVDPLTGGGIYNAMYTGDLAAKVAIQAMGAGKTDAEALMPYDQGWRSSTMG
                     KAIERNYHIKEYLINLPDDKLNAIIHSARSLDLKDFSTFTLIKELMKRNPKLVAELAV
                     LKATIG"
     gene            complement(9008..9184)
                     /locus_tag="PK154_02475"
     CDS             complement(9008..9184)
                     /locus_tag="PK154_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW09960.1"
                     /translation="MLQIHRDVCGYCGCCVSVCPEGALELIDAYLEVDDQCTSCGICA
                     KVCPVGALEVKDED"
     gene            complement(9268..10389)
                     /locus_tag="PK154_02480"
     CDS             complement(9268..10389)
                     /locus_tag="PK154_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPW09961.1"
                     /translation="MTSGLFCDLHIHSCFSIASSSRMLPSCLVEGCRKKGIGVLGSGD
                     SLHAGWRDLWNRIPDTGEVLVVPTAEVEDHDRVHHLIILDSFERFEDLASDLGRHAAR
                     LAETGRPHVRLPGSEIAGRVHDLGGLIGPAHAFTPWTSLYAAFDCLSGCYGAERPDFL
                     ELGLSADSSYGAGISDLAGIPFLSNSDAHSPDPAKLGREFFELTVAEVCIKGVMESIQ
                     AGKVTMNAGFFPEEGKYNRTACTRCYQQYTPAFAASVRWRCPDDQGLIKKGVADRAAE
                     LADAPPTERPPYLHIIPLAEIIRQVIGSSSVATRACTRLYDRFVTPDTDEISVLTRTP
                     IKELAAIDGRVAPAIESFRQGRVSLIPGGGGRFGSFSFF"
     gene            complement(10386..11141)
                     /locus_tag="PK154_02485"
     CDS             complement(10386..11141)
                     /locus_tag="PK154_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HPW09962.1"
                     /translation="MDEIEVQFVDDVPKEELKAPVLIEGLPGIGHVGKLVAEHMIQEL
                     GAKKVAEIHSIFFPPQVIIDEHGTVRLANNELYYYAGETHQFLFLVGDYQATSSEGHY
                     LLADAYLDIAAECGVSRIYTLGGYGVGHLVDEPRVLGAVNFPGLRGMIEDAGGVVGRD
                     EPGGGIIGAAGLLLGLGVSRSIEGICLMGETSGYLVDPRSAASLLKVLSTLLAIEIDP
                     TRLEQRACEMEHVLQKLIDGERGAQDDELRYIV"
     gene            complement(11129..11290)
                     /locus_tag="PK154_02490"
     CDS             complement(11129..11290)
                     /locus_tag="PK154_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HPW09963.1"
                     /translation="MKGRIRRCPVDSAYTLHDHCPLCGAATVTPHPARFSPEDQYGSY
                     RRIARGWTR"
     gene            complement(11287..12075)
                     /locus_tag="PK154_02495"
     CDS             complement(11287..12075)
                     /locus_tag="PK154_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HPW09964.1"
                     /translation="MSTQSWPTAGELVVCTVRDIKDFAAFVSLDEYPGREGLIPISEI
                     ATGWIKYIRDHIREGQKVVCKVLHVDQSRGHIDLSLKDVNDHQRRDKIREWKNESKAR
                     KWIGFAAEHSGENPEVIEAALIKKYGDLYTVFEEIVTEGTDTLKKTGLSAGVIEALSQ
                     VARENVKVPRVTVAGNLILTSTKPDGVNIIRRALRSAEPKIPEAEIELTYLGAPTYRI
                     KVTAPDYKKAEKALEKAAAAAIGVLERSGGEGKFVKKPKSGKAA"
     gene            complement(12084..12272)
                     /locus_tag="PK154_02500"
     CDS             complement(12084..12272)
                     /locus_tag="PK154_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HPW09965.1"
                     /translation="MVQQARANRSRFVKVKCPDCENEQVVFEKACTVVNCVVCSKVLA
                     EPTGGKAKVNVEILETFE"
     gene            complement(12278..12556)
                     /locus_tag="PK154_02505"
     CDS             complement(12278..12556)
                     /locus_tag="PK154_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HPW09966.1"
                     /translation="MKMPVKFKTYCPFCRNHQTHEVEKVKKAKTTGLHWIDRQKARRG
                     SIGNMGKFSKVPGGDKPTKKINVRYRCKTCGKAHLRAGFRAGKFELTE"
     gene            13239..13547
                     /locus_tag="PK154_02510"
     CDS             13239..13547
                     /locus_tag="PK154_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HPW09967.1"
                     /translation="MDTFEQRTRELLPLKKEERWKRYDGMKEACLCSSCPSFTGSAEK
                     NGETLYCLLGMSFGCVRDDRGCICPGCPIYIEHGMMKKDFCMKGSEKDLRWEMEVGTR
                     "
     gene            14140..>14557
                     /locus_tag="PK154_02515"
     CDS             14140..>14557
                     /locus_tag="PK154_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW09968.1"
                     /translation="MKTSVVAGILFLFLIGMVPCAVAEDDASGDQGGTILFASPENGT
                     VWIGSIPTNATIYIDGVDHGLTDRYVTNLTAGTKNLSLVKTGYQNLSTTFEVEADKTV
                     VLPTYILTESAGPEDGTGTVWIGSIPTNATIYIDGVD"
BASE COUNT         2937 a         4244 c         4420 g         2956 t
ORIGIN      
        1 ttcatcttca cccgtgacgc ttccggagag atcaacgggc tcgagatgta cacaatggac
       61 ggctcgtatg acgcatcaga gaaggtgccg tgaagacggg caatgatttt gggattttga
      121 tggtccgaac cacggaccct cctctcccca atctggtaaa acaggcattt tttctcttgt
      181 aacaacagcg gtaaggccgg ggaggcagcg gtccgcctct tccgtgacgg gattttttgg
      241 cctgtccagg agattgcagc agatatttta tctctttaaa gaggggcgat cataaaaaca
      301 gcagatcaca atttttttta tcattcaatc gtaatcccct tcatgaacaa ccctctcctg
      361 cgatatgcat tcctgttcat tgctgcctgt tttgtggccg gcatggtctt tcttaccgga
      421 tgtacaagtg ccccgtcgaa gaccggaaaa ctggacgact ttgatgagtt tgtcaccgta
      481 aaaatggcgg aatatgaagt accaggagcc gttgtcggta ttgttgagaa tgataccgtc
      541 gtatacctga aagggttcgg tgtccgtgaa atcgggcggc ctgaaaaggt cgatcccgac
      601 acccgcttcc agatcgcctc ggtatccaag tatgtcacgg caggtgcaat cggcactctc
      661 gtcgaagagg ggaaactggg ttgggacacc ccggttgtca catatctgcc aggatttgca
      721 ttgaaagaca cgtacgctgg cgggcatgcg acactccggg atctgctcgc tcaccggaca
      781 ggattcaaat cttatgatgg ggaattgctg ggcagggtcg gatactccaa cagcgagatt
      841 ctggaacgga tagagtacct tgagccgggt ggcagtttcc gtgaaaagta ccaatactcc
      901 aatatcggat atttcattgc cggagaagtc gcggcagcag cggacaacca gagctgggaa
      961 gacctgacgg atacccggat cctccggccg ctcaatatga cgcggtcagg agcccacccc
     1021 gaaaccctgt atctcgatga caaccatgtc tccggtcatg gcggggcgcc gggagatgtg
     1081 catatcatac ccctcgagga ggcttccctc cctgctgccg gccaggtggt ctccaccggc
     1141 cgggacatga cacagtggct gcggatgatg ctgaacgagg gctcggtgga tgggaagcag
     1201 atcctcacac caaagaccgt gaaagagatt catgcagcga gcatggtagc aggggcaagc
     1261 ggacctctcc gcgacccgaa tggtgcaacc ggccttggct gcgactcgta ccatttcctc
     1321 ggcgagcggg tcatcgagaa gaacggagcc ctggatggcg tccgttcgat cgtcattctc
     1381 atcccgggga aaaagagcgg tattgtggtg atcgcgaaca agcaactgac ggcattcccc
     1441 gaggcagtgc gggatgaatt cctggaacgt tatatcggga aggctggcat tgacctgcag
     1501 gcccgggaaa aagcttcgca ggcagggtgg aactcgctca tcaagaaccc ggaacggccc
     1561 gcagctgcag gtcctgcaac gatcatttcg tccgccatgg cggggaatta ccgaagcgag
     1621 ctatacggca ccctggaggt gaaccttggc tccgatccag ggaatatgac gatagggctc
     1681 ggcccggaga aatacccggg gaatctcacc cattggacga acaacacctg gtatctctcg
     1741 ttcccaaatc cagacgatca ggtcggatat ttcaccttta ccgagaatac gtcgggatct
     1801 gtcaccggat tcacctctga agaatttggc aggtttaccc gggtatgaac caggaaggac
     1861 gggtgacagc cattcaatat catgaagggc tgtccccggc tttcacccct gttttatttc
     1921 atgcctggta tgaagggcag tatcaacgtg attcgctcaa gtggtatcaa ggatcaaatg
     1981 acaatcaatc ctaatcatgt ggcgacagcc cgattatcgg tgaagggtgc agctgcggtg
     2041 tcttttgtcc agaccggaag tatcaggacg ggttcacagg atgggccggg aatggtatgg
     2101 gccgatcagg tcgggccccg aggcgatgac ctcggcaagc agcggcttga agaggaccgc
     2161 cgcaaggatg gccgcggtga tgatggcgat gagtatcgcg atcatcgcct ttccaaagct
     2221 cgccttgtgg agctcctgta tcccgatcac cccgacgacc accgaccaga gcggggcggc
     2281 aaatgccccg atcactggga tccacccgat caatatcgcc ggggtgagag agtacagggt
     2341 cgccttgatg gtcttttcaa gaccgttcct gccgcatacc atgatgatcc agagatggag
     2401 ccagcaggcg tagaccaccg ccatcaccac cgtcagggca aggatgataa agaacgagag
     2461 gacgacgttc ccgctcccga acccgagccc ggcaaggacg agggccgggt gcttggagac
     2521 ggcgatgccg gagatgatcg tggagaggag ggtgtagatg atcgagatga tgacaaagta
     2581 aatcatggtg tcctgcaggt gctcatcccg cacctgccag aacgcctcgc tcgggctgaa
     2641 aagaaatccc cgtatcttgt cggagatccg gactatcatc caggtacccc tccgctcatc
     2701 cggatcatat ggtatgtact ccttcgtgct gttcgatcat atacctggcg acgaggaggt
     2761 gcctcatagg agcccccgtt ccgcggcaat ctcccgtgtc cgggcgatcg cctccagggc
     2821 atcggctttc ttctggtcga cggcagaggt ggacaggaaa ccggcgaacc gttccctctc
     2881 ctgcgacgcg atccgttccc cggccccctg gcagatcgcc tcccaggtcc gctccgggaa
     2941 gtagagggac tgggcgatcg agaggaggag cccggccgaa gcgggcgaga gcaccccttc
     3001 ccgctctgca gctctgatcg tgcaccggat attgatgagc ggctcggaga ggggcagatt
     3061 cgtctccggg tcatacacga gggcgacttc gtcgtcggag gtcaggatac cgtcccggta
     3121 gagccggaag atcgtcccca ccccctccat gcccagcgga gcgagctcag cggcacggag
     3181 cgcccccatg gaggatgccc cgatcacccg gaccccctgt gcaagggcac caaggatctc
     3241 ccggtgcccg accgagcagt cctggaagaa gacgccgtcg atgagggtga tgatccgggc
     3301 accctctgct gccgcatccg tgatgtcccc gcgttttgcc ggagggcgat agtctgccgc
     3361 gaggagccgg ccggcagcgg cagggtcaag gcttggaccg agaaaaatga cgatcccgtg
     3421 cctcatggca cctcctgccg atccgctccc ggtccatggc aaagacctcg agccccggca
     3481 cgatcaccct gacgaccggg actccgatcg attcccgcgt gaggtccacg acgatcacct
     3541 gcgggaggcc ggcctccttc agccgctccc tggcacgatc gatgtcctcc agaaaatcgc
     3601 tggtgtcaaa ggaagggata tcggaaaagt tcctcgtctc atgggaagag aaccagtacc
     3661 ggttcatccg ctttgtccgt tcgtacccca tcttcttccg gatgtcggcg acagtggtat
     3721 cctccctcgc cccgtggatc tgggtgagcc ggctctgggc aacctcggtc agggcccgga
     3781 ggaccgcgat ccgtgcactg gtatgggtgc ccatgccgat ggtgaggagg gcgggatcct
     3841 gcagcagggt atcgtccgat accgcggcaa tggtcgggat cccgatatcg ctggtgatat
     3901 ccttcaccgt gacctcgact cctgcagctc cgaatttttc gaggagactc ccggcgaggg
     3961 gatcatcgat ctccccgacc tgtggtcccg tatcccgggt gacctccgca agggaccagg
     4021 cgtcccgctc gatgatctcc atcagggcat ggaagaccgc ctcctcgcgg gtattgccgg
     4081 acgcaagtcc gttggtggag gtccggaaga gctgggggta ttcgcggggc agggggtgga
     4141 agaccgcact ggcagggacg aggatctcct cgccggcacc gaggtcatat cccgtgaccc
     4201 acgggatcgg ggcatctgtg atgctcccgg cggggaggat cagccgggca gggtcgagca
     4261 cggagatctc cccgccgcag agccgggaat acggtgagac caggagcgtg cggctgtcca
     4321 gctcggccga gtaccgctcg atcccctcca tcatggcaga gaccttcgcc tcggcggggg
     4381 tggccccttt tccgttgtag accgagacgg cgccaagccc ggcggtcggc cggatactcg
     4441 agaagaccgg tatcccgatc cggtcgaggt tcgtgatatc ggcaacccgg gtaatgccgg
     4501 cggctgggag gacccgctcc acccgtgcga gggtctcttc cggggagacc gtccgctggg
     4561 tatcgttcat gtagaccttt ctgcaggagg agagtaacat cggaaggaag cctcgggaag
     4621 ggtgaatctc tggagaaagg ttcgccggtc tccgagatga gccttttgct tcccttcgag
     4681 cctccctgct gttgtgcagg gatcacggct gcccgtttcg tgccggttcc gtcaatgaac
     4741 ctccgtaaag gggcggcatg caagggagaa aaggtgggac agagcccctg ttttggatcc
     4801 tcgcccgcag gagtgagggg cgcctcttct cagactgcgg tcagactgaa gaggtagggg
     4861 agcatgatca gtgagaagag gatcacgatc atcagggtcg agatgaaggt caccacgtac
     4921 agggaatagc gcccaatccc cctccgttca aacagccggt cgaccccctc tttcaggatg
     4981 tagccgccgt ccagcgggac catcgggatg gcattgaaga gcccgacact gatgttgatc
     5041 cacccgcacc agaagaggaa gtggatgatc ccgaagaacc ctgcccatgg gacggaataa
     5101 aactcctgtg cggcggtatc cagggccagg atccggaggg ggtttccaaa cccgccgata
     5161 tcgaagggga cggtcaggaa cctgaggaac cctatcggtg agacgctgcc tttgatcgtg
     5221 tcgaggacga gggccgggtc atagtaggtg acccccataa acccggtggt ccggttcgca
     5281 agctcctccg gccactccga cagggtcagc tggtaatcgc tgatcacccc ctggtgcccg
     5341 atcgtgaggg tgatcgtgtc cccgggccgg gtgctgttca ggatggcggc gacatcggcc
     5401 ggggtgctga cggaggttcc gttcaccgcg tggataaggg aataggcggg tatccctgcc
     5461 tgttcggcgg aatagttctg gtaaaccccg ccgatgaccg gtccggctgc cggggctgcc
     5521 agccccatca ggaggatcag ggcaagaaag cagagccctc ccaccacgat gttgttcgtg
     5581 atgccggccc caaacatccg gatcttcttc atccccctcg cctggctgag ttcctcctcg
     5641 tcgggctcga caaagaagcc gatggggagg acaaagagga gcgctcccat gctcctcacc
     5701 cggatattct cgacacggga gaggatgccg tgcccgaact cgtggatcgc gatcgtgacc
     5761 acgaaggcga gccagaccgc gagggtagag gggacatact cgttcagccc cgggatcagg
     5821 aggatgttct gcggggcata aatccccgtg ggctcaggcc gcaccgcgaa ggtgtagctg
     5881 agcgaaagga cgaggaggat ggtcatcacc accgatatga caatgaccat cagcacgccg
     5941 aaggttgcgt aggcccggag gaaggtggag tactttcgga accggtcaaa gaagcccacg
     6001 ctcatggtct tgatcgccat gatcgggcca tagaacatga tatgctccga aaagtacccc
     6061 ttctgccgga tgatcagcgc gactgctgcg tagagagcga cggcagtgat gagtacgggg
     6121 agccattcca ttctgtagct atgggtggga cagggacata ataatgatga cccgggaaac
     6181 cgggtgttga aggactcccc gggtcaaggg gattgtccgg actcctgact cccgctgcaa
     6241 aagaacgaga gctcggtctg cccggcacgg ccgaggatct gccgggtcgt ctcccaggag
     6301 gtgcgggcga taggtggcgg ccggccgttc tcgccgatgt aggcctccat gaacgcaatc
     6361 gtggtatcat ccgacggata cccgctcccg atcaccccgt actccccggc aaggtccccg
     6421 atcagccggt cacggaccac ctttgccacg atccctgccg ctgagacgac cgggatggtt
     6481 gcatcggcat ggtgccgggc gatgacccgg cagggatagc cgatgcgggc agcgaccgca
     6541 cgcccgaacc gggcggctac cacatcgcag gcgtccacgt atgcgatcgc ggggtggagt
     6601 tcccggatca cctcggcatg ggcagcggcc atcagctggt tcatggtcat cgagcgccgg
     6661 cggatatcga tctcctcggc cgtgatcaca agcacggtgt agtggacgct ctcctggatc
     6721 cggccaaaga ggtccgtccg cgcagcaggg gagagggtct ttgaatcccg gactgggatc
     6781 gcggcgaggt cctcttcgga ttcgcacccg acacaggcga ccacgagcgg gccgaggacg
     6841 gcccctttcc ctgcctcatc gaccccgcca aacatcagat cccttggtat gatgcgcaag
     6901 ttatttcaat ggcgatgatg gtaaccccac tctatgtaca tcatcatcgt gggcttgggc
     6961 ggtatcggga ggagccttgc agcccagtcg gtggggaacg gggacaacgt cgtgatcatc
     7021 gaccggaacg agcagcggtg cagcgagatg ctcgagcatt acgatgtcct cgcgatcacg
     7081 gggaacgcca ccgacaaggt catcctcgag gacgcgggga tcgaccgggc agatgcgctc
     7141 gtggcgacca ccagcgatga ctcggttaac ctgatgacct gctggcttgc caagcggttc
     7201 aaggtcccga acgtggtctc gatcgtgaac aacgtcgaac actcggacct cttcaaggag
     7261 gtcggggtaa agatcagcga gaatcccgac gagctcgtgg cctcccggct ctattactgg
     7321 gcccggaacc cgatgctcca gcagctcgcc tcgattcccg gggggacgat cttcgaggtg
     7381 gtcgcagaga aaggtgcccc catcgtcgac catgagatcc gcgaactgaa ggtgaaggac
     7441 tttgtcttca tcgcgatccg gaggctgggc ggcgagctga tcatcccgag cgggacgatc
     7501 cggatcaggc cgggggacac catcacggtc ttcaccaaaa aggaggccga ggtcgagacg
     7561 ctcgagctcc tcaaccggca gctcagggtc cctgccacct gaacgggaag gccggcccct
     7621 cccctttcag gggatgtcct ggggagatcc atgaggcggt catgcctttg gtgatgcaat
     7681 acctgcctgc cttttttgag ttcttttacg gtttccggac cggtggatcc ttcgcccttg
     7741 tttcgaacgg aaatcccccc ggtatggaca acgggcggcc gttataggga aaaaacgggt
     7801 gaaaagaaga gcggggtgaa gttacccgat cgttgccttg agcacggcga gttcggccac
     7861 cagtttcggg ttccgcttca tcagttcctt gatcagggtg aaggtcgaga agtccttcag
     7921 gtcgagggat cgggccgagt ggatgatcgc gttcagcttg tcatcgggga gattgatcag
     7981 gtactccttg atatggtagt tccgctcgat cgcctttccc atggtcgagg aacgccagcc
     8041 ctggtcgtag ggcatcaaag cctccgcatc ggtcttcccg gcacccatcg cctggatggc
     8101 gacctttgcc gcgaggtccc cggtgtacat cgcgttgtag atgcccccgc cggtgagcgg
     8161 gtcgacgacc cgtgccgcat cgcctgcgat gatcaggccg tcggcaacgg tccgtggaag
     8221 ggggcggcag accgagactc ccccgacgat gcactcgatg gtcttgccgt tggggaacct
     8281 ttccctgacg aaccggtcga ggtagtcctt tgcacggtga cctgccccgc tcttcttgcc
     8341 cgagatcccg atcccgacgt tcgcggtccg cttccctttc gggaagatcc agaggtagcc
     8401 ttccggcgct acctcgtttc cgaggaagaa ggtcgtgcac tcatggtcga tctcgatgcc
     8461 ggtcatcagg tactggcacg agctcatgat ctcccggacc gggaccgcgg tgtccacccc
     8521 gcaccagcgg gagaacttgg actcgacgcc gtcggccgcg atcgtcacgg cagcctccac
     8581 cttcttctgt tctccgcagt actcgagcac cgcacccttc accctccccc cgtccatcag
     8641 gggtgccgag gcacgggtct tgaccatcac gtcggcacct gcctcggcgg cctgccagac
     8701 gagctcgcgg tcaaagacct tccggtcaag gatgtacccg atcttgctcc cggccatcgc
     8761 cgcctcaagc atcatcgagg atccgtccgg ggcgacgatc tcggcccgct tgagctcgac
     8821 tgcgatccac cgctcgtcag gcttgacgaa ctcggcgagg gcctctttcc ctatcccctc
     8881 cgcacaccgg accggggctc cgatcgccgg gcgcttttcg acgaggagta ccgagtgccc
     8941 ggcctgtgcc gcggtccgtg ccgcgatggc acctgccgga ccgccgccga tcacgagcag
     9001 gtcgtattca gtcctcatcc ttcacctcca gggcaccaac cgggcatacc ttggcacaga
     9061 tcccgcagga ggtgcactgg tcatccacct ccaggtaggc atcgatcagc tcgagcgcac
     9121 cctcgggaca taccgagacg cagcacccgc agtacccgca gacatcacga tgaatctgaa
     9181 gcatctttca tgtataggga aggttcaccc ataatccttt cttgcgggga agggtacaga
     9241 agaaagggca atctctctga tttttggtca gaagaaagaa aaggagccga aacgcccacc
     9301 tccccccggt atgagactga ccctcccttg cctgaacgat tcgatcgcgg gggcgacccg
     9361 gccatcaatg gcagcgagtt ctttgatcgg ggtcctggtg agcaccgata tctcgtcagt
     9421 atccggggtg acaaaccggt catagagccg ggtgcatgcc ctggtggcga ccgatgagga
     9481 gccgatcacc tggcggatga tctcggcgag ggggatgata tggaggtaag ggggcctttc
     9541 tgtgggaggt gcatcggcaa gttcggcggc ccgatcagcg acccccttct tgatcaggcc
     9601 ctggtcgtcc gggcaccgcc accggaccga tgctgcaaag gccggggtgt attgctggta
     9661 gcagcgggtg caggccgtcc ggttatactt cccctcttcg gggaagaacc cggcattcat
     9721 cgtgaccttc ccggcttgta tgctctccat cacccctttg atgcatacct ccgcaaccgt
     9781 gagctcgaaa aactctctgc cgagctttgc cgggtcaggg ctgtgggcat cggagtttga
     9841 gaggaacggg atgccggcaa ggtcggagat gcctgcccca tagctgctgt cggccgagag
     9901 accgagctcg aggaagtccg gccgctcggc cccgtagcac ccggagaggc agtcgaaggc
     9961 tgcatacagc gaggtccagg gagtgaacgc atgggcagga ccgatcaggc cgccgaggtc
    10021 atggaccctc cctgcaatct cgctccctgg caacctgaca tgcggccgcc cggtctctgc
    10081 aaggcgtgcg gcatggcggc caagatcaga ggcgaggtcc tcgaaccgtt caaaggagtc
    10141 gaggatgatc aggtggtgga cccggtcatg gtcctccacc tcagcggtgg gcaccaccag
    10201 cacctctccg gtgtcaggga tgcggttcca gaggtcccgc cagccggcgt ggagtgaatc
    10261 cccgctgccg agaaccccga tccccttctt ccggcatccc tcaaccaggc aggaaggaag
    10321 catccgggac gacgaggcga tggaaaaaca ggagtggata tgcaggtcgc agaagagccc
    10381 gctggtcata cgatgtagcg gagctcgtcg tcctgtgccc cgcgttcacc atcgatcagc
    10441 ttctggagca cgtgctccat ctcgcatgcc cgctgctcga gcctcgttgg gtcgatctcg
    10501 atcgcgagca gggtggagag gacttttagg aggcttgcag cgctccgcgg atcgacaagg
    10561 tatcccgaag tctcccccat gaggcagatc ccttcgatgc tgcgggagac gccaagcccg
    10621 aggaggagtc ctgctgcccc gataatgccc cctccgggtt cgtccctccc gaccacccca
    10681 cccgcgtcct cgatcatccc gcggagcccg ggaaaattga ccgcaccaag gacccgcggt
    10741 tcatcgacaa ggtggccgac gccgtatcct cccagggtat agatccggga taccccgcat
    10801 tcggcagcga tgtccaggta ggcatcggcg aggagataat gcccctcgct cgaggtggcc
    10861 tggtaatcgc ccacgaggaa gaggaactgg tgggtctcgc cggcatagta atagagctcg
    10921 ttattggcga gccggacggt cccgtgctca tcgatgatca cctggggcgg gaagaagatc
    10981 gagtggatct cggctacctt ttttgcgccg agctcctgga tcatgtgctc ggcgaccagc
    11041 ttgccgacat gtccgatccc gggaagcccc tcgatcagga ccggggcttt cagctcctct
    11101 tttgggacat catcgacgaa ctgaacctct atctcgtcca tcctctcgcg atcctccggt
    11161 agcttccgta ttggtcttca ggagagaagc gtgctgggtg gggggtgacc gttgccgctc
    11221 cgcagagagg acagtggtcg tgcagcgtgt aggccgagtc gaccgggcag cgccgtattc
    11281 tccccttcat gccgcctttc cggacttcgg tttcttgacg aactttcctt cgccgccaga
    11341 gcgttcgagg actccgatcg ccgcagcggc tgccttctca agggctttct ctgccttctt
    11401 gtaatcgggt gcagtgacct tgatccggta ggtgggggca cccaggtagg tgagttcgat
    11461 ctcggcttcc gggattttcg gctcggcgct ccggagcgcc cgcctgatga tattgacccc
    11521 gtccggtttt gtggaggtga ggatgaggtt cccggcaacg gtgactctcg gcactttgac
    11581 gttctcccgt gccacctggg agagtgcctc gatcacaccg gccgaaagcc cggtcttttt
    11641 cagggtgtcg gtcccctcgg tcacgatctc ctcaaagacc gtgtagaggt cgccgtattt
    11701 tttgatcagg gcagcctcga tcacttcggg gttctctccc gagtgttcgg ccgcaaaccc
    11761 gatccacttc cgcgccttgg actcgttctt ccactcgcgg atcttgtcgc gccgctggtg
    11821 gtcgttcacg tcctttaagg agagatcgat atgacctctg ctctggtcaa cgtggagcac
    11881 cttgcagacg accttctgcc cctcgcggat atggtcacgg atatacttga tccatccggt
    11941 cgcaatctcg gagatgggga tgagcccttc gcggccggga tattcatcca aggatacaaa
    12001 cgctgcaaag tccttgatgt ccctgacggt gcagacgacg agttctcctg ctgttggcca
    12061 cgactgggtg ctcatcgctc ttctcactca aatgtttcca ggatctcgac gttcactttt
    12121 gccttgccgc cggtgggctc agcaaggacc ttgctgcaga ccacacagtt gaccacggtg
    12181 catgccttct cgaagacgac ctgctcgttc tcgcagtcag ggcatttgac cttgacaaac
    12241 ctgctccggt ttgctcgtgc ctgttgtacc atcctgttca ctccgtcagt tcgaattttc
    12301 cggcacggaa ccctgcacgg aggtgtgctt ttccgcaggt cttgcaacgg taccggacgt
    12361 tgatcttctt ggtgggcttg tccccgccag ggactttcga gaacttgccc atgtttccga
    12421 tgctcccccg ccgtgctttc tggcggtcga tccagtggag accggtggtc ttggccttct
    12481 tgaccttctc cacctcatgg gtctggtgat tcctgcagaa tgggcagtag gttttgaatt
    12541 ttactggcat tttcatgata ttttcctcat gctaccggat agcggatacc attattattt
    12601 aaaccgattg ctatataaga ctgtgggaat ctcctcttcc ctcgcaggca ggggccgtac
    12661 gatcccgctt cagcggaagg attgatgctg cgggatcatg ctgaaaggca gcagacagat
    12721 ctccgaaggg cagtcgaatc ctacgatgca atcctacaat atatcagata tgatccagca
    12781 aatacctacc cgtcataggt gacaccagac atggccattc ccttctgctg acatacattc
    12841 ccttctgctg acatacattc ccttctgctg acatacgaga ggcaatgcct ggacgacctg
    12901 tcaggtacga tacagcaaag gcagatatga tatggccgct ggtttaccct ggattccttc
    12961 tcttaggata cgatacaatt ccaggacgac ctgtcaggta cgatacagga aaggcagata
    13021 tgatatggca gctggttctc agtcagatca gagggatcgc tctgatatat ctcagaaaat
    13081 tgtgttcccg tacgatcctc ccgggagagt aatccccctg cgattccatc agatcctctc
    13141 gcctcatcag caggaatacg ctgcagggac agaggggtcg tgggtcacct gatggtttga
    13201 taaccttatc ctcctatctt ctttcgtgag tcatttccat ggataccttt gagcagagaa
    13261 caagagagct cctcccgctg aagaaggaag agcggtggaa gcggtatgac ggcatgaaag
    13321 aggcctgtct gtgctcttcc tgcccgagtt tcacggggtc tgcggagaag aacggggaga
    13381 ccctgtattg cctgctcggg atgagctttg gctgtgtccg tgatgaccgg ggctgcatct
    13441 gcccgggctg tcctatttac atcgagcatg ggatgatgaa gaaagatttc tgcatgaagg
    13501 ggagtgagaa ggacctgcgg tgggagatgg aggtcgggac ccgctaactc ctggcactcc
    13561 tgtccgtccc ttgcttcacc ctgtcagtca tgcgggatat cggggaggat aacaccctgt
    13621 tctggtagtg cgggcgaaaa ctttttttat atgaaaaagc ctccggtatt ttcatctttt
    13681 atgcttgtgg ccctcaaagg aatcatgtta ttttttcgaa cgatccgtga cccctgccaa
    13741 ttcagtgcag gagagcacag tccgggtggc aacagagccg ccccattctg gtgtgttccg
    13801 atccggccag ccagggggag actgccggga gacaggatgc cgggaaatgc gtcgccagga
    13861 taggggctga acggagacga ttccccatgg tcttcgcgga tatcctgatt gccttgaacc
    13921 caggtacttc ttatgggggc aggtctataa aatatcgaga atactgctga ttacatttat
    13981 atagttaata aacgaggata ctcttatcag attaccaggt acctgactga gtgatagatc
    14041 cgggagagca gggggcggag atgtccatgg gcatgctccc cagggagctc tgatcgggag
    14101 agccagggtg tgatctgtag atatggcaag gtggtaatag tgaaaaccag cgtagtggca
    14161 ggtattcttt ttctctttct gatcggcatg gtcccctgtg ctgtggcaga ggacgatgca
    14221 tcaggggatc agggtggcac gattcttttc gcctctcctg aaaatggaac agtctggatc
    14281 gggtcgatcc cgacgaatgc aacgatatac atcgatggtg tcgaccacgg cctgaccgac
    14341 cggtatgtga cgaacctgac cgccgggacc aagaacctgt ctctggtgaa gaccgggtac
    14401 cagaacctct cgacgacctt cgaagtggaa gcagacaaga cggttgtgct gccgacgtac
    14461 atcctgactg agagtgcagg accggaagat ggcaccggaa cagtctggat cgggtcgatc
    14521 ccgacgaatg caacgatata catcgatggt gtcgacc
//
LOCUS       DAOOCO010000024        14543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3776_length_14543_cov_4.758007, whole genome shotgun sequence.
ACCESSION   DAOOCO010000024 DAOOCO010000000
VERSION     DAOOCO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14543
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_14543_cov_4.758007"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1018
                     /locus_tag="PK154_02520"
     CDS             221..1018
                     /locus_tag="PK154_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW09969.1"
                     /translation="MTDHDFLIPAKFPYVGLYSTAKGISAVGSPVKVKILELLSRQEM
                     AFDKLVENSGRAKSTISIHLKDLAEAGIIDAKPDPEDGRKKIFYLHSLFLGGVDSEER
                     GRFDIERYIKKELPCNGNPATLYRFILTTIRLNLMDQGMGINPVLYRAGSKAGESLYQ
                     CVEGSTIDNIVHRMGKYWVNNGLGKIELERKNPLTLNIRDCFECIDLPIKGKPVCAFE
                     SGVLSSIFSAYYAQRMRAVETRCYAMGSNLCRFELTEYPGQMTIDAS"
     gene            complement(1034..1531)
                     /gene="sixA"
                     /locus_tag="PK154_02525"
     CDS             complement(1034..1531)
                     /gene="sixA"
                     /locus_tag="PK154_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HPW09970.1"
                     /translation="MDLYLLRHGKAEKTVPVGGTDEGRPLTGRGRSEIQSIARWIRSQ
                     GCIFDYIATSPLIRAYETAAIVAEGYPDSDGPVVWEELSPGMECDLLLNRIRKEGSLP
                     SLLLVGHEPSLSNLISVIISGSVDARIVVKKGGLVKIVNFIPDLEPCGDLVWLLFPAL
                     MQKMS"
     gene            1793..2995
                     /locus_tag="PK154_02530"
     CDS             1793..2995
                     /locus_tag="PK154_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09971.1"
                     /translation="MHSAKGEYLKKLTEMHPMPSVGIGKTLEGTSPPSVFIGSWNYPK
                     VYAGPMISPAHGDIRIMDTPESWIPDGLSQDEIIRYRMSLVRGKRLWDVRDLSGRFAE
                     QLRDIALSSISLESEVVFSDTPRGGFAGEQHTPFGPSGYLDSLETSSAGWIRGLEKVY
                     DDTDLTARDAMVHLHAEGFAPSLVQKALSAGVMGRKRDRRLVPTRWAITAFDTIIGNH
                     LLSKVRNCEVIDSFYVHEFSSLHNHYAVILLPVPWEFEWIEAFLHVNGDEEAIFSDYE
                     GNGGKREYSCVGGCYYSSKMAALEALSRERKQAGVIILREARKGYIPMGVFNVRENVR
                     HAMTGKPLDFDDLRSALNHLSHSFQLPVPRFIKESTVLVRSLKERQMRLSDFNPEAET
                     IRGATPTG"
     gene            complement(3054..3138)
                     /locus_tag="PK154_02535"
     tRNA            complement(3054..3138)
                     /locus_tag="PK154_02535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3100..3102),aa:Leu,seq:tag)
     gene            4011..5471
                     /locus_tag="PK154_02540"
     CDS             4011..5471
                     /locus_tag="PK154_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPW09972.1"
                     /translation="MKRLGHLAIIAHDMGLIFEFLALITLVPIIVLILFREWEMIIPM
                     GAVPFIFIVTGLLISRVPKKAYTPSLSVALVAVALTWLAIAAVGAVPFVLALDLSWTD
                     SIFESMSGWTGTGFTMMTSLDTMPKTLIFWRSFMQWVGGIGVIAFGIAMLSRSGLTQI
                     QLYRSEGRSEELMPSVVSTGRRMWAIYVFLTVVFTGMVLLSGIPLWDAINLVMVSLAT
                     GGFAPHDAGMFYYNNPLLEALLIPVMLAGALPFKLYFLIYRGKFRMFFRDSVVRLILA
                     LSLIGSLIVSLDLFLFSNLPVSEAIRVGTFCTISGYTCTGLQNSLLHWVPLPLIVITL
                     LMMIGGAAGSTSGGIKTNRVILGYEGLVWWFKRLFVRGSVIVPFKHEGRNIPTRISEV
                     ELSKNMLIIILYVLTIFIATIVAFHLASTSFSVYQVVFELVSAASNVGLGLGYITAAS
                     PMSVKWLFIFVMWIGRLEIIPVFILLMGIVRGFEAR"
     gene            5599..6087
                     /locus_tag="PK154_02545"
     CDS             5599..6087
                     /locus_tag="PK154_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09973.1"
                     /translation="MPADPLHILIAALVCWLNFVTIDILFKLPERGGVSGAEEIGRHI
                     EKGGGALHGGYMMGNIVSSPDASAGTLLASCGVYAAGIPGGLLAALLVYIGNRICHDP
                     GYAGTTGCITATLIVYGFTLIGFSAADFIIGMVIAILTIQGISHVHASRLLSTLWRWR
                     EG"
     gene            6084..6338
                     /locus_tag="PK154_02550"
     CDS             6084..6338
                     /locus_tag="PK154_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 domain-containing protein"
                     /protein_id="HPW09974.1"
                     /translation="MNSTEIALAVCGIIALYAAFRVMLEKDTMKKLPFLNVISFAISG
                     SIVLLVPHPLAIVAAAAYFVGSTLESNAIASTFAGGPRNE"
     gene            6331..6561
                     /locus_tag="PK154_02555"
     CDS             6331..6561
                     /locus_tag="PK154_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HPW09975.1"
                     /translation="MNEILMLMFGAMVILGTLATVISRDLFDKLISLGIIVAGIMPFL
                     ADRGLLDVLTATALIAPLSTLFILMAVRRKAD"
     gene            6558..6794
                     /locus_tag="PK154_02560"
     CDS             6558..6794
                     /locus_tag="PK154_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HPW09976.1"
                     /translation="MNPEFVLGLTLILIGTVASAFPRPKTYLVQLINLEIPAWGLLLV
                     MLSYNEALALLTFGGVSALSTFIYVRVIQKQEGT"
     gene            6791..7234
                     /locus_tag="PK154_02565"
     CDS             6791..7234
                     /locus_tag="PK154_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2106 family protein"
                     /protein_id="HPW09977.1"
                     /translation="MIIQRISRIISNYEMIMPIFAGAVTLLFVVAIFSLPLPYYEEQL
                     YPKTYDPTSPLNPYDRGGPPFIQIDIKEQYPENSPMAGYVTSYLTPFSWYMANTTLYL
                     GTTIVAHPGGILDEILYNTRGLDTVVEPTILFIAFAIASYLYRKG"
     gene            7237..7839
                     /locus_tag="PK154_02570"
     CDS             7237..7839
                     /locus_tag="PK154_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2105 family protein"
                     /protein_id="HPW09978.1"
                     /translation="MWQEYSIGLLLVFIAAAIAFFALVIEKDDLHRLLLLDLVEVPSL
                     GIIALLGTDLAEALILPGLVVGIAELLALSQIYVTKEGLRERKVQGLKIEVIQADAPL
                     IISVLLICYGVILSGFTGGGVAGLGVVFYFMARGHREHFELLEMASGISWAIWIFAFF
                     VFMFIPEYWLLALFMAAIGILLKVIVKMSLVGTMWGDGNG"
     gene            7832..8488
                     /locus_tag="PK154_02575"
     CDS             7832..8488
                     /locus_tag="PK154_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09979.1"
                     /translation="MDNPGGIPYFILEFGDELVYFSPYTVSLFILALVFTLFAVIAKP
                     ERQVDIVFGGDAFYAKETSLAELKFRRFMAIACGFASMGAMVTGDLFNFTLFISLVGI
                     CNVGIVAAVKNRHVQNAAYQYGIVALSATVPLFGAAALIAATTGTLSMVELWSNASLA
                     VPLVVKALLVLGVMGEGMAPFYAAKAEMFRAPGAPYVIMCSLSSLLIFLRVIEVVLVI
                     "
     gene            8488..8697
                     /locus_tag="PK154_02580"
     CDS             8488..8697
                     /locus_tag="PK154_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09980.1"
                     /translation="MTKKKDILRALGLLTGAIMLVGLILQQGGILPLAGYEAVLVVCF
                     PIFVICVFLWWMAREHGEDIPFIGY"
     gene            8699..9541
                     /locus_tag="PK154_02585"
     CDS             8699..9541
                     /locus_tag="PK154_02585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPW09981.1"
                     /translation="MIGYLLFAVTFGLLLLGIHRKAIARIQRRPGPPVWQEILHMLKF
                     SFKSTWIPATASDTLFVGVVLVAIGIWSAALFVLLAGGSILIIFGIYMLHKIVEHGFG
                     LSSGSPYGKFGGVRSVISAASEIPLFVSVAVVALYTQSLDISSIIQYQNVHGPLLFIV
                     PLAAVAMYLVILSKMPFGPFSIVESKELVSGYKTEHFGVWRAGLQVSNGLKTYVLLFL
                     FITVFMGGIPLWWTLAGMVVLTLSLSFVCALSPMLSPFDSVTVQMVITGIMVVYAAVL
                     GVFA"
     gene            9538..9780
                     /locus_tag="PK154_02590"
     CDS             9538..9780
                     /locus_tag="PK154_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09982.1"
                     /translation="MIREILLGAGITYLVILVIQIGKRPSTALGALAAASLILIATGC
                     YWYSRHESVHKWEIVMLWAAILLFVIYGIMKFLGVL"
     gene            9813..10166
                     /locus_tag="PK154_02595"
     CDS             9813..10166
                     /locus_tag="PK154_02595"
                     /inference="COORDINATES: protein motif:HMM:NF020780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 family protein"
                     /protein_id="HPW09983.1"
                     /translation="MKYAILTSTRHDQVVRSIAAELNIRQIKLRKWMMDHFDMMLLEN
                     LPARYDQGRMAKEMEPPLEKEIGAALYTRAVPLLSDGEMERIKKEVYALVKEGRPEHD
                     AVLAGREMIRDVILQ"
     gene            10163..10618
                     /locus_tag="PK154_02600"
     CDS             10163..10618
                     /locus_tag="PK154_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09984.1"
                     /translation="MSLLQSVKNLVRSRSIHVAYVDVGSCNGCDIEVLACLSPRYDIE
                     QYGIYVHNNPREADILLVIGAYTPGWEDKLYTLWEKIPDPKAAIAIGNCPISGCLFNR
                     PHSLVDPPVSKHIPIAAEVPGCPPRPTEILSAVLSLAPLVFKDYEVAKR"
     gene            10615..11703
                     /locus_tag="PK154_02605"
     CDS             10615..11703
                     /locus_tag="PK154_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPW09985.1"
                     /translation="MKRTVDVSIPIGPIHPCFKEPARVKCETKGEFVLSAEVELGYMK
                     KGIERIMRGRPWQEVMFLAERVCGICSVIHNMVFIEALEQMSAIFVPERAALLRVIVN
                     ELDRMQSHILANFSYCYTIEHETLGMYLLDVREHVMDELERITGARVTCAYIVPGGVR
                     FDLRHKDTLHLRANLAAIEGEVVRYMHMFESGPLIALRSRGIGVLTREAALEACAVGP
                     TARASGMPEIDRRLRHPTYQKLGFTPVSRTEGDNYARIMVRFQEVLESIAIIRRCLDM
                     LPEGPIRGGGIPGAGEIAYSGEAPRGELSYFIKTDAAGRVLDIAIQTPSIMNIEACCH
                     YMLKGVASLADVPSTFVSADPCIACTER"
     gene            11705..12316
                     /locus_tag="PK154_02610"
     CDS             11705..12316
                     /locus_tag="PK154_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW09986.1"
                     /translation="MPLPILPYLCEIVRLEWVRKFFFVKTAPLVTPSHFRGFPELTGK
                     TCTHALFCMMACPAPGAIDVIKTTEGWEPRIHKGHCIRCGLCVEACPNGVLTSGRILE
                     SMFYDHTRLSFSFRIDVDTNLCMGCGNCAVACPVNKLIDAQMAYGGRASTDQLLLKVS
                     EGKCHALHPELCTGCKTCENHCPNEAIRVSRLLEAVQVDEEEI"
     gene            12313..12702
                     /locus_tag="PK154_02615"
     CDS             12313..12702
                     /locus_tag="PK154_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HPW09987.1"
                     /translation="MKFLLNTGRTIRQGSYVERKNSVAYQEEATSLCMNPVDMMNLEL
                     EEGSQVVVESEQGSVVMKVHPASWITQGEVFVCLGPYANYLVNPETHGTGMPDFKGTM
                     VTITPTSAPLVTVAELMERCGGVRYED"
     gene            12692..13981
                     /locus_tag="PK154_02620"
     CDS             12692..13981
                     /locus_tag="PK154_02620"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HPW09988.1"
                     /translation="MKIEDVVCPFCGCLCDDLVVEVEGTRVVSVENGCTLANEKLMGD
                     HRLKTPIRRGGKGWKETTYDEAIRETAEILCDADRPLLYGWSGTHGEAQSIGIHLAEI
                     LGALVDNTSTVCHGPSILAIQEVGHPGCTLGQVKNRADVIIYWGTNPIDAHPRHMSRY
                     TTFADGFFLKEAFRDRKLIVVDVRKSESANIADEYIRIQPGGDYAVLSALRAIVQGKR
                     GIIPSSVSGVSREQLFRVADLLLSAKFGAIFFGLGLTMSRGKSKNIRNAIELTEELNR
                     HTKYIISPMRGHWNVYGSNEVFTWITGYPYAVDFARGIAFYNPGETTSVDVLSRKECD
                     ACLVVASDPGAHFPKACAEHLAEIPTVQIDPHVNATTHLSRIQIPVAVTGIDAEGTAY
                     RMDGVPIRTRMLFKGTYPSDTDVLNQLYAVVKEIKGP"
     gene            13978..>14543
                     /locus_tag="PK154_02625"
     CDS             13978..>14543
                     /locus_tag="PK154_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW09989.1"
                     /translation="MSEFLIRNAFVIDPLSGIKGEIMDIPIRNGRIAEDVGSSAEVID
                     ADRCLTLPGGIDSHSHVCGTKVNFGRYMSPEDMRAGRTSRKGVMHVTSGYSVPTTFGN
                     SYRYSVMGYTTVLEGAMAPLEARHTHEEFTATPHQDMLANTLFDGNWSIMEAVREKDV
                     KRAAAVVAWTLSAVKGFAIKLTNPGGTES"
BASE COUNT         3371 a         3656 c         3809 g         3707 t
ORIGIN      
        1 atcgcacagc gtcccgcaga agggacagac cacatcgggt attgtcttgg tcatcgggta
       61 ttcacatcct gtatgcagag tgcagtaccg tgcgggaatg tccggaccgt accggtgccg
      121 cactctgaac acatgttcta tggtatacga acattttgtg ttatacgaac taaattaata
      181 tatacttttt gatccttatg atgtataaga agggcctgct atgacggatc acgacttcct
      241 tatccctgcg aagttccctt acgtaggtct ctattccacc gcgaagggga tcagtgcggt
      301 aggaagtccc gtaaaagtga agattctgga actgctctcc aggcaggaga tggcatttga
      361 caaactcgtt gagaattcag ggcgggcaaa gtcaaccatc tcaatacatc tcaaggatct
      421 tgcagaagca gggattatcg atgcaaagcc agatcctgaa gacggccgga aaaaaatctt
      481 ttatctgcat tccctctttc tggggggggt agattcagaa gagcgtggcc gttttgatat
      541 cgagcgttat atcaaaaaag agctcccctg caacggcaat cccgcaacgc tctaccggtt
      601 tattctcact acaatccggc tgaaccttat ggaccaggga atgggcatta atccagtact
      661 ctatcgtgca ggctcaaaag caggagagag cctttaccaa tgtgtggagg gtagtaccat
      721 cgataatatt gttcaccgga tgggaaagta ctgggtgaat aatggattgg gaaagattga
      781 gctggaacgg aagaatccac tgacactgaa tattcgggat tgctttgagt gcattgatct
      841 cccaatcaaa gggaaaccgg tatgtgcgtt cgaatctggt gtactctctt ccattttctc
      901 ggcatattat gcccagcgga tgagagcggt tgagacccgc tgttatgcga tggggagcaa
      961 tctctgccgg tttgaactga ctgagtatcc cgggcagatg acaatagatg cctcatgagg
     1021 aggcggaacg gcatcaactc attttctgca tcagggcggg gaatagtaac cataccaggt
     1081 caccacaagg ttccaggtcg ggaataaaat tcacaatttt taccagtcct ccttttttca
     1141 ccacaatgcg ggcatcaacc gaccctgaaa taatcacgct tatcaagtta ctcaaagaag
     1201 gttcatgccc gacaagaagg agcgacggca aagatccctc ttttcgaatg cggttaagca
     1261 gaagatcaca ttccattccc ggggacagtt cttcccatac caccggacca tcagaatccg
     1321 gataaccttc agcaacaatt gcagctgttt catatgccct tataagcggg cttgttgcga
     1381 tataatcaaa gatgcaacct tgagaccgga tccatcttgc tatgctctgg atttctgatc
     1441 tacctcgtcc tgtgagcggc ctcccttcat cagttccccc aactggaact gtcttttcag
     1501 ctttcccatg ccgcaggaga tatagatcca ttacagagtg atattgtagg cacgacttat
     1561 ggattttccc aaaaaagtac acccgggaca atcccggttc gatcgaagga tcaaaggggt
     1621 attcatgttc cttgctacat ttttcattgt gttcagacag accaatcgga taaagcgaat
     1681 tatcattaat gtgaaaaagc ggtgttttaa tttctggctg ttcaggagga tacttatcac
     1741 tttctccctg cgcccggcag tttttttaaa atctgttccc gatgactgac agatgcattc
     1801 agccaaaggg gaatatctca agaaacttac ggaaatgcat cccatgcctt cggtcgggat
     1861 cggaaagacg cttgaaggta cttctcctcc ctcagttttt atcgggagct ggaactatcc
     1921 caaggtctat gcaggtccta tgataagtcc tgcccatggg gacatcagga tcatggacac
     1981 gccggaatcc tggatcccgg atggcctctc ccaggatgag attatcaggt accggatgag
     2041 tctggtgagg ggaaagcgat tatgggatgt gagggatctt tcagggcgtt ttgctgagca
     2101 gttgagagat attgccctct catcgatatc acttgagagt gaggtggttt tttccgatac
     2161 cccgagagga ggctttgcag gtgagcagca tacccccttt ggtcccagcg ggtatcttga
     2221 cagtctggag accagttctg caggctggat acgtggcctc gaaaaggtct atgacgacac
     2281 ggatctcact gccagagatg caatggtcca tctgcatgca gaaggattcg ccccatccct
     2341 ggtgcagaaa gcattgtcag ccggcgttat gggaagaaaa agagaccgga gacttgtccc
     2401 gacacgatgg gcaatcaccg ccttcgatac gataatcgga aaccacctcc tttcaaaagt
     2461 acggaattgt gaagtaatcg actcatttta tgtgcatgag ttttcaagcc ttcacaatca
     2521 ttatgcggtg atcctcctgc ctgtcccctg ggaatttgaa tggatcgagg cgtttcttca
     2581 tgtaaatggc gatgaagagg cgatattttc tgattatgaa gggaacggag gaaaaagaga
     2641 gtactcctgt gtcggagggt gctattattc cagcaaaatg gcagctcttg aggcgctcag
     2701 cagagagaga aagcaggcgg gtgtgattat cctccgcgaa gcccgaaaag ggtatattcc
     2761 catgggagtc ttcaatgtaa gggagaatgt ccggcatgcg atgaccggaa aacctcttga
     2821 ttttgatgat ctccggtctg ccctgaatca cctctcacac tcatttcaac taccggtccc
     2881 ccggttcatc aaggagagca cggttcttgt cagatccttg aaagagcgac agatgcgatt
     2941 gtcggatttt aatccggagg ctgaaacgat taggggagct actccaacgg gatgaggaat
     3001 acgcttgacc tggatcgctg ttgacaaaag cttcccgtta ccggacagca tcatgcgagg
     3061 ggccggattc gaaccggcga actcctgcga gaacggaccc taaatccgtc gcctttgacc
     3121 tggctcggca accctcgcac cctttggtaa ggtactatac tcttctgatc gtttattagc
     3181 tatccttgaa ggcaggagtg ttttggaatg tgagagacaa gtcgggtatt gtgaccattc
     3241 atattgtacg atttcatttc actggtaaag aacatctttg cgtatttttc tgaacaagag
     3301 gtttttttgc gtagtagttt ctctttaatt ccatgcacga tcaagagaaa tcaggtaatt
     3361 ttatctgatc cataagttcc tatcgcagaa ttattattct gcgcaaatat tataaacaag
     3421 gagattgaat agaaatatgc catatatgag ggcctgggag ctgtgcagta ccgggtttat
     3481 taccttgtct acagcaccag caaggtatgg catgttcagc atcctggaat accggatata
     3541 ttcatgacag gcagaaggat cacatcccca catgggcttg gaacatatcg cggctcttcg
     3601 ccaccttgca gcctgcgatt attcgatggt gattattccc tcctacgagc gaatcagcct
     3661 gtcacactga gcaaaaagga acgctggcat gaccccatca tacccggtaa ccggccttta
     3721 ttctatcata tatggtaccc ttttctgctt tttaacaaag cataagggtg aagggtatac
     3781 tcaatgccgg atctgcaacg gcatggacca ttcaatggcc agatacctcc taaacccctt
     3841 ccctttagcc ctcatatcac atacaattcc tctctgaaat tctcgtgctg agtctctttt
     3901 gcaatgtaat gtttcaacaa cttctttcgg aaaggtatgg aattttctta catccgcttt
     3961 ttttagagcg aagtataatg ctgttggatc acgacacact ctaactatca atgaagcgcc
     4021 tcggtcatct ggcgattatt gcgcatgata tggggcttat ctttgagttc cttgccctca
     4081 tcaccctagt ccctattatt gtcctgatcc tgttcaggga atgggaaatg attatcccga
     4141 tgggggcggt cccctttatt ttcattgtaa ccggcctcct tatctcgaga gttccgaaaa
     4201 aagcatacac tccctctctc tcagttgccc tcgttgccgt cgctcttacc tggctcgcca
     4261 tcgcagctgt tggggcggtc cctttcgttc ttgcccttga tctctcctgg acggatagta
     4321 tcttcgaatc tatgtcaggc tggacaggca caggatttac catgatgaca tcactcgaca
     4381 caatgccaaa aaccctgatc ttctggcgtt catttatgca atgggtaggt gggattggtg
     4441 tgattgcatt tggcattgcc atgctatccc gatcagggct cacacagatc caactctatc
     4501 gctcggaggg tcgctcagaa gagctgatgc ccagtgtcgt ctctaccgga agaaggatgt
     4561 gggcaatcta cgtgtttctt accgttgttt ttacagggat ggttcttctc tccggaatcc
     4621 ccctctggga tgccataaat ctggtgatgg tttcactggc gacaggaggt tttgcccccc
     4681 acgatgcagg tatgttttac tataataatc ccctgcttga agcactcctc atccccgtca
     4741 tgctcgccgg ggcgctcccg ttcaaactgt atttcctgat ctaccgggga aaattccgga
     4801 tgtttttcag ggactccgta gtccgcctga tcctcgccct ctctctcatt ggatcgctca
     4861 ttgtatccct ggacctcttc ctcttcagca atctgcccgt ctcagaagcg atcagagtag
     4921 gaacattctg cacgatatcc gggtatacct gcaccggact tcaaaattcg ctcctccact
     4981 gggttcctct ccctctcatt gtaatcaccc tccttatgat gattggcgga gcagcgggga
     5041 gtacttcggg agggataaag accaacaggg tcatccttgg ctatgaaggg cttgtctggt
     5101 ggttcaagcg actctttgtc aggggaagtg tcattgttcc cttcaaacat gaaggacgga
     5161 atatcccaac aaggatatcc gaagtggaac tctccaagaa tatgctgatc atcatcctct
     5221 atgttctgac aattttcatc gcaactattg tggcattcca cctggcatct accagtttca
     5281 gtgtatatca ggtcgtattt gagctggtgt ctgcggcaag caatgtaggc ctggggcttg
     5341 ggtatatcac tgctgcaagc cccatgagtg taaaatggct ttttatcttt gttatgtgga
     5401 tcggcaggct tgagatcatc ccggtgttca tccttctcat ggggattgtc cggggatttg
     5461 aagcaaggta aatggattcc gggttaacaa tcagccgcat taaaattacc atattttacc
     5521 gattataaca gccattttgg tcaaatacaa acctttattg agaaaacacc gctacctgtc
     5581 tacactggag attggaagat gccggcagac ccattgcata ttctgatcgc cgctttggtg
     5641 tgctggctaa acttcgtaac aatcgatatt ctcttcaaac tccccgaacg gggtggagtc
     5701 agcggggcag aggagatagg caggcatata gagaagggag gaggtgccct tcatgggggg
     5761 tacatgatgg gaaacatcgt gtcatcccct gatgcatcgg cagggaccct tcttgcctcc
     5821 tgtggtgttt atgcagccgg aatcccggga ggcctcctcg cggcactcct tgtgtatatc
     5881 ggaaaccgga tatgccatga tccgggatat gccgggacca cgggatgtat caccgcaacc
     5941 ttaatcgtct atggtttcac cctcatcggg ttctcggctg ccgatttcat catcgggatg
     6001 gtgatagcca ttctcacgat ccagggcatc tctcatgtgc atgcaagccg tctcctctcc
     6061 actctctgga ggtggaggga aggatgaaca gtaccgagat cgcccttgct gtctgtggaa
     6121 tcatcgccct gtatgctgca ttccgggtca tgctcgagaa ggatacgatg aagaagctgc
     6181 cgttcctgaa tgtcatctcc ttcgcgatat caggctcaat cgtcctcctc gttcctcatc
     6241 ctcttgcgat cgttgcagca gcggcatatt ttgtgggatc cacacttgaa tcgaacgcaa
     6301 ttgcaagcac atttgccgga ggtcccagga atgaatgaga tactcatgct catgttcggg
     6361 gcgatggtta tcctcgggac ccttgccacc gtcatatccc gggatctgtt tgataaattg
     6421 atctccttgg gaatcattgt agccgggatc atgccgttcc tcgcagaccg tgggctgctg
     6481 gacgtactca cggcaactgc gctgatagcc ccgctatcaa cgttgtttat cctaatggca
     6541 gtcaggagga aggcggattg aaccccgaat ttgtccttgg attgaccctg attctcattg
     6601 gaaccgttgc atctgcattc cctcgcccaa agacctatct ggtccagctt atcaacctgg
     6661 agatacctgc gtggggcctc cttcttgtga tgctctcata caacgaggcc ctcgccctcc
     6721 ttacctttgg aggagtatct gccctctcga catttatcta cgtcagggtg atccagaaac
     6781 aggagggaac atgatcatcc agcggatctc gcggatcatc tcgaattatg aaatgattat
     6841 gccgatcttt gcaggagcag tgacactcct gtttgttgtt gcaatattct cccttcccct
     6901 cccctattac gaagagcagc tatatcccaa gacctatgat cccacaagcc ctctcaatcc
     6961 atatgaccgg ggaggacctc cgtttattca aattgatatt aaggaacagt atcctgaaaa
     7021 ctcccctatg gctggctacg taactagcta tttaaccccg ttttcctggt atatggcaaa
     7081 taccaccttg tatctgggaa cgacgatcgt tgcacaccct gggggaatcc tcgatgagat
     7141 cctctacaat acacgggggc tcgatacggt ggtcgaaccg accatactgt tcatagcatt
     7201 tgcgatcgct tcgtatcttt accggaaggg atgagcatgt ggcaggagta ttcgatcggg
     7261 ttgctgctgg tttttatcgc cgcggctatc gccttttttg cgcttgtcat tgagaaggat
     7321 gacttgcacc ggctactcct cctggatctg gtggaagtcc cctcccttgg gatcatcgcc
     7381 ctccttggta ctgatctggc tgaagccctg atcctcccgg gattagtggt cggaatcgct
     7441 gagctcctgg ccctttccca gatctacgtt accaaggaag gattaaggga aaggaaagtc
     7501 caggggctga agatcgaggt aattcaggca gacgcacccc tcatcatatc cgtccttctc
     7561 atctgttacg gggtaatact gtcaggtttt actggtggtg gcgttgcagg acttggcgtg
     7621 gtcttttact ttatggcaag aggacaccgg gaacatttcg aactcctgga gatggcaagt
     7681 gggatatcct gggctatctg gatctttgct ttctttgtct tcatgttcat cccggagtac
     7741 tggctcctgg cactcttcat ggcagccatt gggatcctcc tgaaggtgat agtaaagatg
     7801 tcccttgtcg ggacgatgtg gggtgatggg aatggataac cccggaggga tcccatattt
     7861 catccttgag ttcggcgatg aactggtata cttcagccca tataccgttt ccctgttcat
     7921 cctggctctg gtattcaccc ttttcgctgt gattgcgaag cctgagcgcc aggtggacat
     7981 agtctttggg ggtgacgcgt tctatgcaaa ggagacctcc cttgcggagc tcaaattccg
     8041 gaggttcatg gcaattgcgt gtggctttgc ctccatgggt gcgatggtga caggggacct
     8101 cttcaatttc acccttttta tctctctggt aggcatctgt aacgtcggta ttgtcgcagc
     8161 agtgaagaac cgccatgtcc agaatgccgc ataccagtat gggattgttg ccctctctgc
     8221 caccgtcccg ctttttggtg cagcggctct catcgcagca acaaccggga ctctctcgat
     8281 ggttgagctc tggagcaacg catccctggc tgttcccctt gtggtgaaag cacttctcgt
     8341 tctgggggtg atgggagagg gcatggcgcc gttttatgca gccaaagcag agatgttcag
     8401 ggctcccggt gcaccgtacg tgatcatgtg ttccctctca tccctgttga tatttctaag
     8461 ggtaatcgaa gttgtgctgg tgatctgatg acaaagaaga aggacattct tcgtgctctg
     8521 gggcttctca ccggggcaat catgctcgtc ggtctgatcc tgcaacaagg cggaatactc
     8581 cctctcgcgg gatacgaagc ggtccttgtc gtatgctttc cgatctttgt gatctgtgtg
     8641 tttctctggt ggatggcgcg tgaacacggg gaggatattc cgttcatagg atactgacat
     8701 gataggctac cttctcttcg cggtaacatt cggcctcctt ctgcttggaa tccaccggaa
     8761 ggcgattgca cgaatccagc gacgtcccgg cccgcccgtc tggcaggaga tactccatat
     8821 gctaaaattt tcctttaaga gtacctggat ccccgcgact gccagcgata cgctgttcgt
     8881 aggtgtggtg cttgttgcaa tcggcatctg gagcgctgcc ctgtttgtcc tgcttgcagg
     8941 gggaagcatc ctgatcatct ttgggattta tatgcttcac aagatcgtgg agcatggatt
     9001 cgggctctct tcaggttccc cgtatggaaa attcggcggg gtgagatcgg tgatatcggc
     9061 agcatccgag atacctcttt tcgtcagtgt tgctgtggtg gcattgtata cccagtcgct
     9121 cgacatctcg tcgataatcc agtatcagaa tgtccatggc cccctcctgt ttatcgttcc
     9181 gcttgccgcg gtcgcaatgt atcttgtaat cctctcgaaa atgccattcg gaccgttctc
     9241 aatcgttgag agcaaagagc tcgtcagcgg ctacaagacc gaacacttcg gagtttggag
     9301 agcaggtctt caagtcagca acggtctgaa gacctatgtc ctcctgtttc tgttcataac
     9361 ggtgtttatg gggggcatcc ctctctggtg gacactcgcc ggcatggtgg tcctcaccct
     9421 ctctctctcc tttgtttgtg cactgagtcc catgctctca ccgttcgata gcgtcaccgt
     9481 ccagatggtg atcaccggga tcatggtggt ctatgccgca gttctcgggg tgtttgcatg
     9541 atccgggaga ttcttcttgg agcaggtatc acctatctgg taatcctggt aatacagata
     9601 ggcaagagac cttctaccgc acttggagca cttgctgcag cgagtctcat tttgatagca
     9661 acggggtgtt actggtacag ccgacacgag agtgttcata aatgggagat cgtcatgctc
     9721 tgggctgcga tcctcctgtt cgtgatctac ggaatcatga aatttctggg tgtgctatga
     9781 ccgggtatct ctacgaaaag gatctctcgg ctatgaaata tgcaatactg accagcacac
     9841 ggcatgacca ggtggtacgg tcgattgcag cggaactgaa tattcgtcag atcaagctcc
     9901 gcaaatggat gatggatcat ttcgatatga tgcttcttga gaaccttcct gcccggtatg
     9961 accaggggcg gatggcaaag gaaatggaac cccctctcga aaaagaaatc ggtgccgccc
    10021 tctatacccg ggcggtgcca cttctgagcg acggcgagat ggagcggata aaaaaggagg
    10081 tatatgccct ggtgaaggaa gggagacctg aacatgatgc tgttctggct ggacgggaga
    10141 tgatcaggga cgtgattctt caatgagcct cctccaatcg gtgaagaacc tggtccgctc
    10201 acgatccatc catgtggcct atgttgatgt cgggtcctgc aacggttgtg acatcgaggt
    10261 gctggcatgc ctctctcccc ggtatgatat cgaacagtat ggcatctacg tccataacaa
    10321 tccgagggag gcagacatcc tgcttgtcat tggtgcctac accccgggat gggaagataa
    10381 gttatacact ctctgggaga aaatacccga cccaaaggct gccattgcta tcggcaactg
    10441 ccccatatca gggtgcctgt tcaaccgtcc gcatagtttg gttgatcccc cggtttcgaa
    10501 gcacatcccg atcgcagcag aagtacctgg atgtcctcca aggcccaccg agatactctc
    10561 tgcagtgctc tcgttagcac cgctggtgtt caaagactac gaggtggcta agcgatgaag
    10621 cggacagtcg atgtatcaat cccgatcggg ccgattcatc cctgtttcaa ggaaccggca
    10681 cgggtaaaat gcgagacgaa aggcgagttt gttctctcag cagaggtgga actcgggtac
    10741 atgaaaaagg ggatagagcg gattatgcgg gggagaccct ggcaggaggt catgttcctc
    10801 gccgaacggg tctgcggtat ctgctctgtg atccacaaca tggtcttcat cgaggcgctc
    10861 gagcagatga gtgcaatctt tgtgccggag cgggcagccc ttctccgtgt tatcgtcaac
    10921 gagctggacc ggatgcagag ccatatcctt gccaattttt cgtactgcta taccatcgag
    10981 catgaaaccc tgggaatgta tctcctggat gtccgcgagc atgtgatgga cgagctcgaa
    11041 aggataaccg gtgcccgggt cacatgcgcc tacattgtgc ctgggggagt tcggttcgat
    11101 ctccgtcata aggatacgct ccatctgagg gccaacctgg cagcgatcga aggtgaagtg
    11161 gtccggtaca tgcacatgtt cgagagcggc cccctgattg ccctccggag caggggcatc
    11221 ggggtgttga cccgggaggc agccctcgag gcatgtgcag tcgggccaac tgcccgggca
    11281 agcgggatgc ccgagattga ccggcgttta cgacatccta cctatcagaa actcggattt
    11341 actcccgtat cccggactga aggggataat tacgccagga tcatggtccg gttccaggag
    11401 gtgctggaga gtatagcgat aatccggaga tgccttgaca tgcttccgga aggcccaatc
    11461 cgcgggggag ggattcctgg cgcaggggag atagcgtatt caggggaggc tccccgcggg
    11521 gaactctcct acttcataaa gacggatgcg gcaggaaggg tgcttgatat cgcgatccag
    11581 accccctcca tcatgaacat cgaagcatgt tgccattaca tgctgaaagg ggttgcctca
    11641 ctcgcggatg tcccatcgac ctttgtgagt gcagatccat gtatcgcttg cacggagcgg
    11701 tgatatgcca ctccccatac ttccttatct ctgtgaaatt gtccggctgg aatgggtccg
    11761 aaaatttttc tttgtaaaaa cagcgcctct ggtcacacct tctcacttca ggggatttcc
    11821 ggaactcacc ggcaagacct gtacccatgc gctgttctgc atgatggcct gccctgcacc
    11881 cggggcaatc gatgtgatca aaacaacgga gggatgggaa ccgagaattc acaagggtca
    11941 ttgcatcagg tgcggcctct gtgtggaagc atgcccaaac ggggtcctga caagcggtcg
    12001 gatcctcgaa tccatgtttt atgaccatac ccgcctctcg ttctcctttc gtattgatgt
    12061 cgataccaac ctctgcatgg gatgcgggaa ttgtgccgta gcatgtcctg tgaacaagtt
    12121 gattgacgcc cagatggctt atggcggccg tgcctccacc gatcagctcc tgctcaaggt
    12181 aagcgaggga aagtgccatg cccttcatcc ggaactctgt accgggtgca agacctgtga
    12241 aaaccattgt ccgaatgagg cgatccgggt ctcccgactc cttgaagcgg tccaggtaga
    12301 tgaggaggag atatgaagtt ccttctgaat acggggcgga ccatcaggca gggttcttat
    12361 gttgaacgga agaactcggt ggcatatcag gaggaggcaa cctcactttg catgaaccca
    12421 gtggacatga tgaacctgga actggaagaa ggatcgcagg tagtggtaga gagtgaacag
    12481 ggtagtgtgg taatgaaagt ccatcccgca tcatggatca cgcaaggcga ggtttttgtt
    12541 tgtctcgggc cgtatgccaa ctatctcgtg aaccctgaaa cccatggaac ggggatgccc
    12601 gatttcaagg gaacgatggt aaccattact cctacctctg caccactggt gactgtcgct
    12661 gaactgatgg aacgatgcgg aggggtccga tatgaagatt gaagatgtcg tctgcccgtt
    12721 ctgcggctgc ctctgtgacg accttgtcgt ggaagttgaa ggaactcgcg ttgtcagtgt
    12781 cgagaacgga tgtaccctgg caaatgagaa attaatgggt gatcaccggt taaaaacccc
    12841 tatccgcagg ggaggaaagg gatggaaaga gacaacgtac gatgaggcca tcagggagac
    12901 cgccgagatt ctctgcgatg ccgatcgccc tcttctctat ggatggagcg ggacacacgg
    12961 tgaagcccag agcatcggga tccaccttgc cgagattctc ggagctttgg tggacaatac
    13021 ctcgacagtg tgccatggac catcgatcct tgccatccag gaggtcggac accccggctg
    13081 taccctcggg caggtcaaga accgggctga tgttatcata tactggggaa caaatccaat
    13141 cgatgcgcac ccaaggcaca tgagcaggta tacaaccttc gccgatggat tctttttaaa
    13201 agaagcattc cgggaccgga agctcattgt agtcgatgta aggaagagcg aatctgccaa
    13261 catcgccgat gaatatatca gaattcagcc tggaggagat tatgcggtcc tctccgccct
    13321 tcgggcaata gtccagggaa aacggggtat catcccttcc tcagtttcag gggtatcacg
    13381 ggagcaactg ttccgtgttg cagatctcct cctctcagca aaattcggtg caatcttctt
    13441 tggtcttggc ctcaccatgt cgaggggaaa gagcaagaat atcaggaatg caatcgaact
    13501 cacggaagag ctgaaccgtc acacgaagta catcatctct cccatgcggg ggcactggaa
    13561 cgtatacgga tcaaatgaag tatttacctg gattaccggg tatccgtatg ccgttgactt
    13621 tgcacggggc attgcctttt acaaccctgg agagacgacg agcgttgacg ttctcagtcg
    13681 gaaggaatgt gatgcatgtc ttgtggtagc gagtgatccc ggtgcgcatt tcccaaaagc
    13741 atgtgccgaa caccttgcgg agatcccgac cgtccagatc gacccgcatg tcaatgcaac
    13801 cacccacctc tcccgtatcc agatcccagt tgcggttacg ggcatcgatg cagaagggac
    13861 tgcataccgc atggatggcg taccgatacg aacccgtatg ctgttcaaag gaacgtatcc
    13921 cagtgatacc gatgtactca atcaattata cgccgttgta aaggagataa aaggaccatg
    13981 agcgagttct taatcaggaa tgcgtttgtc atcgaccccc tttccgggat aaaaggggag
    14041 atcatggaca tcccgatccg caatgggagg attgccgagg acgttgggag cagtgcggaa
    14101 gtgatcgatg cggatcgctg cctcacgctt cccgggggca tcgattcgca ttcccatgtc
    14161 tgcggaacga aggtcaattt cggacggtac atgagccctg aagatatgcg ggcaggcaga
    14221 acctcacgaa agggcgtgat gcatgtcacc tccggctaca gtgtaccaac gacattcggc
    14281 aactcgtacc ggtacagtgt catgggatat acgaccgtgc tggaaggggc tatggcaccc
    14341 cttgaagcac ggcataccca tgaagaattc accgctaccc cccaccagga catgcttgcc
    14401 aatacccttt ttgatggaaa ctggtcgata atggaggcag tgcgggagaa ggatgtgaaa
    14461 cgtgctgccg cggtcgtggc atggacactc tctgcggtga aggggtttgc catcaagctg
    14521 accaatccgg gggggaccga gtc
//
LOCUS       DAOOCO010000025        14451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3803_length_14451_cov_4.694429, whole genome shotgun sequence.
ACCESSION   DAOOCO010000025 DAOOCO010000000
VERSION     DAOOCO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14451
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3803_length_14451_cov_4.694429"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK154_02630"
     CDS             <1..322
                     /locus_tag="PK154_02630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW09990.1"
                     /translation="VKSLLDRGYSILGVSGRIDEAAEKFEAELDLIITLAETETSLRR
                     LGSEIQMTRRRVNALEQVLIPELKRQAKYIEITIDERDREDLYRLKKVKKIIERKKKV
                     VKEA"
     gene            complement(326..655)
                     /locus_tag="PK154_02635"
     CDS             complement(326..655)
                     /locus_tag="PK154_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09991.1"
                     /translation="MPDVTDDERGRRVFQIHKEMAVETAIEKIRQTMGQDWKLYSVSD
                     IDILKYMLGESWVSMDRQSWGKCTFSRLGREDIDAIIRIGKQVKSRKRMEGDAVIEIN
                     EILKRIS"
     gene            complement(1118..1450)
                     /locus_tag="PK154_02640"
     CDS             complement(1118..1450)
                     /locus_tag="PK154_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW09992.1"
                     /translation="MPEVSKTEIGRRFFKMQKEKHVEKAIEKIRQAQGSEWILYTQSD
                     IEALKQVLGDVWTFMDRDSWDQISFTRLSGIELRELIRFGRDSLDNIISGKSAAEQSM
                     PILLKKGL"
     gene            1649..3553
                     /locus_tag="PK154_02645"
     CDS             1649..3553
                     /locus_tag="PK154_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPW09993.1"
                     /translation="MIELAFLILFPAIVAFLLLAFKNGQVRNVVVKIAAGLIGLATII
                     LLITYFSSGMVLFSVHLETAAEIMFVISLLIAGFILYLGIKQRKYLAVILVTIQTLMF
                     IGIEGWLAKDVQQVSNLFIDQFSLIMAAIVGIIGTLICVYALAYMETYHAHHPEIPDR
                     QNVFFFMVFVFLAAMFGLIFSNNLLWVYFFWEITTLASFILIGYSKTSESTNNAFLAL
                     EMNLLGGIAFAGAIIYLLIADPTGSLLGIEQLLSSGQAFALVPAVLLSFAGITKAALM
                     PFSSWLVGAMVAPTPVSALLHSSTMVKAGVYIIVRFAPVLSGTTEGLIIGLVGGFTFL
                     LGSAIAISQSNAKKVLAYSTIANLGLIVACAGVGTYKLVWAAILLIIFHAIAKSLLFL
                     CVGTVEHQIGSRNIEDMSGLIVRMPKVAVMMLVGIAGMFLAPFGMLISKWVAIEAFIE
                     AQFGLIFVAILAFGGSITVFFWSKWMGKIISVDPHVTREADTLKSEKRVVLYILAGLT
                     VLICLIFPLISSSLIEPFVLSVYGQTARLSQDNIIIMAMMLFLLMIMPFSMLLPRNGA
                     RKVTPYMGGRPASSDMKFLGSMGVTRDVTLSNYYLNDLFGENRLLMAGTALGFGLILL
                     AMIVLVGMIL"
     gene            3550..4416
                     /locus_tag="PK154_02650"
     CDS             3550..4416
                     /locus_tag="PK154_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPW09994.1"
                     /translation="MMSPVYGLIYILLAPIAGGLVAGIDRKITARMQGRVGPPLLQPF
                     YDVGKLFEKENLVVTRSQDLYALCYLVFMLVAGALFFAGGDLLLVIFAFTLSHIFLVL
                     GAYASSSPYSFIGAERELIQIIAYEPMIILSAVGMYMVTASFFVSDIVVSSIPIVLYL
                     PGVFIGFLMVLTIKLRKSPFDLSTSHHAHQEIVKGVTTEFTGPNLGKIEIAHWYEYVF
                     LLGFIFLFFAFSLPIALLAIVVAYLLEILIDNTFSRVKWQFTLKSAWIIAGMLGLINL
                     AVLYYLSGGLQA"
     gene            4413..4880
                     /locus_tag="PK154_02655"
     CDS             4413..4880
                     /locus_tag="PK154_02655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPW09995.1"
                     /translation="MTYLKRSPWIIHYDASSCNGCDIEVLACLTPMYDVERFGIVNTG
                     NPKHADIFVVTGSINEQNREVVKNIYEQMPDPKVVVAVGICAASGGVFRECYNVSGGI
                     DKIIPVDVYVPGCAARPESIIDGIVKSLSILEEKRIKMNSPAAKKDTPSGGIQ"
     gene            4877..5257
                     /locus_tag="PK154_02660"
     CDS             4877..5257
                     /locus_tag="PK154_02660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPW09996.1"
                     /translation="MKIEEQPTTVIAVAHLIGRVEQFRKGGYRLVQMNATQVGDEFEI
                     NYSFEKDYRFENLRLIIPAETEVPSISGMYWGAFVYENEMHDLFGIAVKGMNIDFNGN
                     FIRTKVRYPFRNPTFQGEGPCQRK"
     gene            5242..6321
                     /locus_tag="PK154_02665"
     CDS             5242..6321
                     /locus_tag="PK154_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPW09997.1"
                     /translation="MSKKIVVPFGPQHPVLPEPIHLDLVLEDETVIDVVPSIGYIHRG
                     LETLIAKREFTEYVYIAERTCGICSFIHGMTYCQGVEQIMDLEIPARAHYLRTIWSEY
                     SRLHSHLLWLGLFADAMGFENLFMNSWRLREFVLDVLEETTGGRVIQGSCKVGGVRRD
                     ISNEKLAAMAGDLKSFETELSDLSEVFLHDDSLKSRLVGVGTLSRTDAYDLGAAGPVA
                     RGSGVAIDLRKTGYAAYRELRFEPATESAGDCYARCAVRIRELFSSIDLIRQAVEKMP
                     EGEIEVKLKGMPDGEYFSRSEQPRGELVHYLKGNGTKHLVRSRIRTPTLTNVPSLVKM
                     LQGCELADVPVIVLSIDPCIGCMER"
     gene            6327..6722
                     /locus_tag="PK154_02670"
     CDS             6327..6722
                     /locus_tag="PK154_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW09998.1"
                     /translation="MGYFEMAKTAIKTVMSRPATLMYPARPGKMTTLSRGHVVIDVTR
                     CISCGMCMKKCPAEALCIDKEAKTWTIDRLRCVVCNSCIEVCPVHCLTMEGHYSSAVL
                     THSGLETFTITYVKPERPKKNESGEPAEA"
     gene            complement(6779..7456)
                     /locus_tag="PK154_02675"
     CDS             complement(6779..7456)
                     /locus_tag="PK154_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HPW09999.1"
                     /translation="MGSYLKDLHEIEESARSVQKLMKERYDCGGSSQDPHPIFTIAGA
                     DAAYGEGYGIGVVVLLAYPSLAIIEHGYAKRAVRFPYIQGLFAFRELPLIMAAYEKIG
                     KIPDLLMVEGHGYAHPQRYGYACHAGAILGIPTIGIAKHPIRCDTMKHPGNERGMQQE
                     MLMGGEVVGVALRTRTGAPPVYVSAGYQTTLHFACQIVLETTRLHRLPEPVWIADLLS
                     KKYLNFL"
     gene            7647..8426
                     /locus_tag="PK154_02680"
     CDS             7647..8426
                     /locus_tag="PK154_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPW10000.1"
                     /translation="MVDQHEEFKTIVLEQAPDYYSELSEYLEVLSNSQRLKILKILER
                     RPKDVRMIASDINTSYENTKKHLDKLISMGLIRKEVGLGQQTSKGVHPVWKYSLVPGA
                     MEAIVRNLGIFSNLRINIDDAGLKKKLEEVRDAVEDEIAGHKPMAILLGGPDDGKLYI
                     LEDRESRVGRVDPSYQAVEGDVVLSSEYSSVSRISKPHAIIKMEGTNFIFEDRGSAGG
                     SFLNSSELQKKQPAILHDGDLIELAKGAKGARLLIILPDSD"
     gene            8447..>9101
                     /locus_tag="PK154_02685"
     CDS             8447..>9101
                     /locus_tag="PK154_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW10001.1"
                     /translation="MKTTPALLLVLVIILTGLFIGTASAADYYVIMADAGPGGSIVPA
                     DEVLVPAGGTQIFTIIPNANYEISDVVVNGVSLGPRSVYTFYNVNSDHVISARFSPLT
                     GSLSISSDPAGASVYIDSQYVGRTKARDALIIDNIPVGTRTVELILKGYIPWSTQVVV
                     SKDEVSAIPMVALTPITPPPTTIPTTTVPTTTIPTTTVPTTTIPTTTVPTTTIPTTTV
                     "
     assembly_gap    9102..9201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9202..10961
                     /locus_tag="PK154_02690"
     CDS             <9202..10961
                     /locus_tag="PK154_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPW10002.1"
                     /translation="PTTTVPTTTIPTTTVPTTTIPTTTVPTTSTTTQTTTTASPTKTT
                     TTVPTTIPTTVPGTSGTSVATTTPSASPSPVTNTPGTGEVTNVPSLNETNIPMPASNQ
                     TTAPVTIEINGTGRGNNTALSFFPPIPGKLNPFIVILLSGGLCLVPLITDTVIQPMPS
                     VQFRAQRKMTTGLFYLGIVAGIIGAAFYTYHSGLLSDTIPPFIAILLPVSAYLVISAL
                     VLTVGTLLSKSLKYTVSGHLLISLIGVFIAALALIATNVEYRLPLLILFSCALAGAVV
                     SRRESRDLRAMPVAENPVLPTETIIAPRSPTIPTSFPKELLEKYSDPQLIGMGGVARV
                     FKAINNQNGGEVALKIPVQFNETSGKSFMKEIKAWEDLSHENIVKIHEVNILPVPYVE
                     MEYLGSSLADLQKPLPPGDAARIIRDIARGLSYAHSQGIIHRDIKPQNILMTEDGIPR
                     ITDWGMSKVMGTGMLPTITGFSLSYAAPEQITPGKFGDTDQRTDIYQLGVVFYELLTA
                     ELPFEGDDISRVSVRILNDTPPPPSVLNPDARPFDGIVAKCLEKDPANRYQSIKGLMD
                     DIEQVLVTQIDIERYEIFE"
     gene            complement(11323..13383)
                     /locus_tag="PK154_02695"
     CDS             complement(11323..13383)
                     /locus_tag="PK154_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPW10003.1"
                     /translation="MAQKHMLSEDEGYRLLQEFGIPVPDHAVTQNIQEARAAAARIGY
                     PVVLKVVSPSIVHKSDAGGVVTGINTEEGLEEAFNGIFRKVSAHLPDAPISGIIVEKE
                     VSKGLELFIGGKTDPAFGRVLSCGLGGTTIELFRDFSLRVLPVGRAHLTDMIRQLRSY
                     PLISGYRGKPPLDEEELIRCLENAARMFANGQISEFDINPLILYEQGACAVDARIYRE
                     SGRDSSIMEKTNSDPDLFHPRSIAVIGASADPKKIGYAVFRNLLPFPGKLFPVNPNLG
                     EVLGHPAYRSVSDIPDEVDAAVIAVPSIAVPGLIEELAVHGTKLAIIISSGFRELGDE
                     GRRMEEKIRDTARKAGMRIVGPNCLGIVLPHLKINTTFDPTTPRKGNIGFISQSGAVI
                     TTIVDWSVPEEIGFSVIFSVGNQVDLGFIDFIRFAAAEPETRAIILYIEEIKEGVSFL
                     HELKETTKSIPVITLKSGSSTHGKKAAASHTGSLAGDYEVYQAALTQAGAIPVFSLRE
                     AFDVAELLVSEGYPKGNRAVVISTAGGFGVLASDYAERFGVMLPSPSPVLLQELDRFL
                     PRLWNRDNPLDIIGDGGADRFARVFDILIRFQNEWDIAIIIAVPSAVLDPTQLALEIT
                     RFSAHTQNVVIGCMIGGESMKGGLGVLRNHHIPNYDDIENAFKAIGRSLVAVKNRDNR
                     DDQL"
     gene            13511..>14451
                     /locus_tag="PK154_02700"
     CDS             13511..>14451
                     /locus_tag="PK154_02700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW10004.1"
                     /translation="MEKFESYARFHHEFSLREFRNRCCPEEELSPLLVALLPTLSRLS
                     FGWRGTGRDYWVFGISPRKPLILSPPSALALTGQIRQGLEFERLQDLTQHYIENKTGK
                     SWDDPLVLQRIRTGIMQQKGQYWSEKDSRRIGYRKGYAVLAYLAYHAPIYFTQFQYAF
                     LMLIKDGLIPHNARILDIGSGPGVVSLAISAISRALPEFSAELFALERSEEFHECYRY
                     FISQFPGKESGITAYPVINGDLRDIKTLLLPSSVDLIVLQNVTNELVQETPEQRSEYL
                     KQASRILAPNGCMIIIEPAELRNSTELRKMVRAATGP"
BASE COUNT         3562 a         3631 c         3426 g         3732 t
ORIGIN      
        1 cgttaagagt ctcttggatc gtggttacag tattcttgga gtgagcggtc ggattgacga
       61 agctgcagag aaattcgagg cagagctgga tctcatcata acacttgctg aaactgagac
      121 ctcactccgg agactaggga gcgagatcca gatgacccgg cgaagagtta atgctcttga
      181 acaggtcctc atcccagaac tgaaacgaca agccaaatac attgagatta cgatcgatga
      241 gcgggaccgc gaagatctct accgtctcaa aaaagtgaag aagatcatcg aacggaagaa
      301 gaaagtggtg aaagaggcct gaatattacg agatcctctt caatatctca tttatctcaa
      361 tgactgcatc cccctccatc cgcttcctgc tcttcacctg ttttccgatc ctgatgatag
      421 catcgatatc ctcccgtcca agtcttgaaa acgtacactt cccccagctc tgacgatcca
      481 ttgaaaccca gctctcccca agcatgtatt tcaggatatc aatatcagaa acagaataga
      541 gtttccagtc ctgccccatc gtctgtctga tcttttctat ggccgtttcc acagccatct
      601 ctttatgaat ctggaagacc cgccgtcccc gttcatcgtc ggtcacatca ggcatatgaa
      661 ttcaacttac gtagaggaat aagtaggatt taaatttttt gaggtttctt tgatttcaga
      721 aggaatggat ggctcgtgtc agtaatgtaa taattcattt agttatatca atttattcat
      781 gattccgata ttattgttcc cgtttcaacg caggcttctg tttcaataat tttttaggat
      841 aaggtaaaag atcaggaatt ccctctgttg ccttttctgg caatcctgtc gtaaatacac
      901 ataagatgtt cagtactttt atccaagctc gaccttcctg atctcattgt ctgtatcatc
      961 attgttaact ctctgtatgc cttcatgtca ggatactcct tttttagaga agttgttcct
     1021 caactccaat taactaaaat aataaaaaca aattggaata tatgtatatg ccatgcattc
     1081 ggaaatgcaa accttattga gtaccccaac aaaaggatta taatcccttt tttaacagaa
     1141 tcggcatact ttgttcggca gcagacttgc cactgatgat attatcaaga ctatctcttc
     1201 caaagcggat aagttcacgt aattcgattc ccgaaagccg ggtaaatgag atctggtccc
     1261 aggaatcccg atccatgaat gtccatacat ccccaagaac ctgcttgagt gcttctatat
     1321 cgctctgagt ataaaggatc cattcagatc cctgtgcctg cctgatcttc tcgattgctt
     1381 tttcaacgtg tttctctttc tgcatcttga agaaacgtcg tcctatctca gtttttgata
     1441 cctcgggcat atcccagtcc cctccctgtc gggtatggct gaattgtctg ttccagtata
     1501 aaataagtgc gtcaggagga gaccctgcag ttcccggctg aaccatttga ggatttataa
     1561 caccgaatca gaaaaaataa ctcatttgtt agtttagaat gattaatgat gtaagaattt
     1621 caaatctgat ggaatagatg gttttacgat gatagaactg gctttcctca tcctgttccc
     1681 ggccatagtc gcatttctcc tgcttgcatt caaaaacggg caggtccgga atgtggtggt
     1741 aaaaattgca gccggactta ttggactcgc aaccatcatc ctgctcatca cctatttcag
     1801 ctcaggaatg gtgctgttct ccgtgcatct tgaaacggca gcggagatca tgtttgttat
     1861 ctccttactc attgcgggat tcattttgta tctgggcata aaacagagaa aatacctcgc
     1921 agttatcctt gtcacaatcc aaaccctcat gtttatcggg atcgagggct ggcttgcaaa
     1981 ggatgtgcaa caggtttcca atctttttat agaccagttc tcgcttatta tggccgcgat
     2041 tgttggcatc atcggtacac tcatctgtgt atacgctctt gcctacatgg aaacatacca
     2101 tgctcatcat cctgaaatac cggatcggca gaatgtcttt ttcttcatgg tatttgtgtt
     2161 tcttgctgca atgttcgggc tcatcttctc caataacctt ctctgggtct atttcttctg
     2221 ggagatcact actctggcat cgttcatcct gataggatac tctaagacat cggaatctac
     2281 caacaatgca ttccttgccc tggaaatgaa ccttctcggc ggaatcgcat ttgccggagc
     2341 tatcatctat ctcctcattg ccgatccaac cgggagcctt ctcggcatcg aacagctact
     2401 gagctccggc caggcgttcg ctctagttcc cgccgtcctc ctcagtttcg caggaattac
     2461 caaggctgcc ctcatgccct tctcatcgtg gcttgtcggg gcaatggttg cacccacccc
     2521 ggtctctgcc ctccttcatt ccagcaccat ggtcaaggcc ggagtctata tcattgtgcg
     2581 gtttgctccc gtcctctcgg gaactacaga aggactgatc attggacttg tgggggggtt
     2641 taccttcctg cttggttcag ccatcgccat ctcgcagagt aatgcaaaga aggtgcttgc
     2701 ctactcgacc attgcaaatc tcggcttgat tgttgcatgt gccggcgtcg ggacctataa
     2761 actggtctgg gctgcaattc tcctcatcat cttccatgcc attgcaaagt ccctcctctt
     2821 cctctgcgtt ggaaccgtgg aacaccagat cgggagcagg aatatcgagg acatgtccgg
     2881 gctgatcgta cggatgccga aagttgctgt gatgatgctg gtcgggattg ccgggatgtt
     2941 cctcgctcca ttcggcatgc tgatctcgaa gtgggtggcg atcgaggcat tcatcgaagc
     3001 acaattcggc ctgatcttcg tggcaatcct tgctttcggt ggatcgataa ccgtgttttt
     3061 ctggtccaaa tggatgggaa agatcatctc tgttgatcca catgtgacga gagaggcgga
     3121 taccctgaaa agtgaaaaac gggtagtact ctatattctg gccgggctca ctgttctgat
     3181 ctgcctgatc ttccccctga tatcatcatc cctgattgaa ccattcgttc tctcggtata
     3241 tggtcagaca gcccgactct cgcaggacaa tatcatcatc atggcaatga tgctcttcct
     3301 cctgatgatc atgccatttt caatgttgct tccccgaaac ggagccagaa aagtaacccc
     3361 ttacatgggg ggccgtcccg ctagtagtga tatgaagttt ctcggatcga tgggagtgac
     3421 gagggatgtc accctgtcca actactacct gaacgatctc ttcggggaga accgccttct
     3481 gatggccgga actgcacttg ggttcgggct tatcctgttg gcaatgatcg tccttgtggg
     3541 gatgatacta tgatgtctcc tgtttatggc ctgatataca tcctgctcgc tcccattgcc
     3601 ggggggttag tggcaggtat tgacaggaag atcaccgcac gcatgcaggg gcgggtagga
     3661 cccccgttgc ttcagccgtt ttacgatgtt ggtaaactgt ttgagaagga gaacctcgtt
     3721 gtcacgagga gccaggatct gtatgcgctc tgttacctcg tctttatgct cgttgcggga
     3781 gcgctgttct ttgcaggtgg ggatctgctc cttgtcatat ttgccttcac actgtcccat
     3841 attttcctgg tgctgggggc ttatgcttct agttctccct acagtttcat cggagccgag
     3901 cgtgaactga tccagatcat agcctatgaa ccgatgatta tcctttcggc agttgggatg
     3961 tatatggtga cggcaagctt ttttgtgagt gacattgtag tctcttccat cccgattgtc
     4021 ctctacctgc caggtgtctt catcggattt ttgatggtcc tgacgatcaa acttcgaaaa
     4081 tctccctttg atctctccac ttcccatcat gctcaccagg agattgtcaa aggagtgacg
     4141 accgagttca ccggtccaaa cctcgggaaa attgagatag cccactggta tgaatatgta
     4201 tttttgcttg ggtttatctt cctgttcttc gcattcagtc ttccaatcgc cctgcttgcc
     4261 attgttgtcg cgtaccttct ggagatcctg attgacaaca ccttcagccg ggtgaaatgg
     4321 cagtttaccc tgaaaagtgc gtggattatt gccggtatgc tggggctcat caatctcgcg
     4381 gtcctttact acctgtcagg agggctccag gcatgaccta tctcaaacgt tctccctgga
     4441 tcattcacta cgatgcatca agctgcaacg gctgcgatat cgaagtcctt gcctgcctca
     4501 ccccgatgta tgacgtcgag cggttcggta tcgtgaacac tggcaatccc aagcatgccg
     4561 acattttcgt ggtcacgggg agcatcaacg agcagaacag ggaagtcgta aaaaatatct
     4621 atgagcagat gccggatccc aaagtcgtcg tcgcggtggg catctgtgca gcatctggcg
     4681 gggtattccg tgaatgttat aatgtctcgg gaggcatcga caaaataata cctgtcgatg
     4741 tctacgtccc cgggtgtgca gcccgccctg aatctatcat agacggaatt gtcaaatcgc
     4801 tcagtattct tgaggagaaa cgaatcaaaa tgaacagtcc tgctgcgaaa aaggataccc
     4861 cgagcggagg gatccaatga aaatcgaaga gcagcccact acggtaattg ccgtcgcaca
     4921 cctgatcggc agggtagaac aattccgcaa gggggggtac cggctggtcc agatgaatgc
     4981 gacccaagta ggggatgagt ttgagatcaa ttatagtttt gagaaggact accggtttga
     5041 aaacctcaga cttattatac ccgcagaaac cgaagtgccg agcatcagcg ggatgtactg
     5101 gggagcgttt gtgtatgaga atgagatgca tgacctcttt ggaattgcag taaaagggat
     5161 gaacattgat ttcaatggga atttcatcag gacgaaagtc cgctatccat tccgtaatcc
     5221 aacgtttcag ggggagggtc catgtcaaag aaaatagtgg tcccattcgg accccagcat
     5281 cctgttttac cagaaccaat acacctggat ctcgtgcttg aagacgaaac agtcattgat
     5341 gtcgtacctt ctatcggata catccaccgg ggtcttgaga cccttatcgc aaaaagagaa
     5401 tttaccgagt acgtatacat tgcagagcgt acctgtggga tatgcagttt catccatggg
     5461 atgacctatt gccagggagt tgaacagatc atggaccttg agataccagc cagggcgcat
     5521 tatctccgga ctatctggtc tgaatattca aggctccatt cacatcttct ctggcttggt
     5581 cttttcgctg atgcgatggg ctttgaaaac ctctttatga attcatggag gctcagggaa
     5641 ttcgttctgg atgttttaga agagaccacc ggagggaggg tgatccaggg cagttgtaag
     5701 gttggcggtg tccgcaggga cataagtaat gagaagcttg cagcaatggc cggagacctg
     5761 aagagtttcg agacggaact gagtgatctg tctgaggtat tcctgcatga tgattcactc
     5821 aaatcaaggc tggtcggtgt aggcactctt tcacggaccg atgcctacga tcttggtgcg
     5881 gcaggtcctg tcgcgagagg aagcggggtg gcgatcgatc tcaggaaaac agggtatgct
     5941 gcctatcggg aactccggtt cgaaccggca accgagtcag ccggggattg ttatgcccgt
     6001 tgcgctgtca ggatccgtga acttttcagt tccattgatc tgatccggca ggctgttgaa
     6061 aagatgccag aaggggagat cgaagtgaaa ttgaaaggaa tgcctgacgg ggaatatttc
     6121 tcccggtccg aacagccacg gggcgaactt gtgcactatc tgaaaggaaa tggcacaaaa
     6181 catcttgttc gctcgcggat acggaccccc acccttacca atgtaccttc ccttgtgaaa
     6241 atgcttcagg gttgcgaact ggcagatgtt cctgtgattg tcctcagcat cgatccctgt
     6301 atcggatgca tggagcggtg agcacgatgg gatattttga gatggcaaaa acagcgataa
     6361 agaccgtgat gagcaggccg gcaacgctga tgtaccctgc ccggccgggt aaaatgacga
     6421 ccctctcacg ggggcatgtg gtgatcgatg tgacccggtg tatctcctgc gggatgtgca
     6481 tgaagaaatg tccggcagag gcactctgta ttgataagga agcaaagaca tggacgattg
     6541 atcggctccg ctgcgtggtc tgcaactcct gtattgaagt ctgcccggtt cattgcctta
     6601 ccatggaggg gcattattca tctgccgtcc tcactcacag tggattggaa acgttcacca
     6661 tcacgtatgt caagccggaa cgcccaaaga agaatgaatc cggagagcct gccgaagcat
     6721 gagaagtggt ttcgtgtttt tttttgatgc tcaagtggga gcatctatcc cttacctgtc
     6781 agaggaaatt cagatatttt tttgaaagga gatcggcgat ccataccggc tcaggaaggc
     6841 gatgcagtcg tgttgtctcg agcacaatct gacaggcaaa atgaagagta gtctgatatc
     6901 ctgctgaaac gtaaaccgga ggagcccccg tccttgttct caaggccacc cctaccacct
     6961 ctcctcccat taacatttct tgctgcatac ctcgctcatt tcccggatgt ttcatcgtat
     7021 cacagcggat ggggtgtttt gctatcccaa tggtgggtat accgagaata gctcctgcgt
     7081 gacaggcata cccatatcgt tgcgggtggg catatccatg gccttcgacc atgaggagat
     7141 ccggaatttt tccaatcttt tcataggcgg ccattatcag agggagttcc cggaatgcaa
     7201 acaatccctg gatgtaagga aaccgtacag cccgttttgc atatccatgt tcgatgattg
     7261 cgagggaagg ataggcgagt agcacaacaa ctccgattcc atatccttct ccatatgcag
     7321 catccgcgcc tgcaattgta aagattggat gaggatcttg agaagaaccg ccacaatcgt
     7381 aacgttcttt catcagcttc tgaacagatc gggctgactc ctcgatctca tgaagatctt
     7441 tcaggtatga tcccatgccc accctgattt atgtgttctg ttcctggtta ttttaaggat
     7501 atttcgagcc cgggaaaaaa aaccaatggt attctgattc tcttactaca ttgattgtat
     7561 gaaccaaccg gttatatcat tgaatagact gtgcggagaa tgtttttgat tgagcaggac
     7621 gacttactat agaagaggca ggcttgatgg ttgatcagca cgaagagttt aagactattg
     7681 tgcttgaaca ggcgccagat tactatagcg aactgtctga atatcttgaa gttctctcaa
     7741 atagtcagcg cctcaagata ttaaaaatcc tagaacgccg gccaaaagat gtacggatga
     7801 ttgcatctga tatcaacaca agctatgaga atacaaagaa acatctggat aagctgatca
     7861 gcatgggact tatccggaag gaggtaggac tcgggcagca gacttcaaag ggtgttcatc
     7921 ctgtatggaa atattccctt gttccagggg caatggaggc tattgtccgg aacctcggaa
     7981 tcttctccaa cctccggata aatattgacg atgccgggct gaagaaaaaa ctggaggaag
     8041 taagggacgc ggtagaggat gagatcgcag gacacaaacc gatggctatc ctgctcggag
     8101 gacctgacga tgggaaactc tatattctcg aggatcggga gtcccgggta ggaagggttg
     8161 atccttcgta tcaggcggtt gagggggatg ttgtactttc atcggaatat tcttcggtga
     8221 gtcggatttc aaaaccgcat gccatcatta aaatggaggg tactaatttt atctttgaag
     8281 acaggggaag cgcaggagga tcgtttctga acagttccga gttgcagaaa aagcagccgg
     8341 caatccttca cgatggcgat ctcattgagc ttgcaaaggg tgcaaaaggt gcccggctcc
     8401 tgatcatact gccagacagc gattaacatc tctcaaagaa tccgctatga agactacgcc
     8461 ggcattgctt cttgttcttg ttattattct taccggattg tttattggca cagcttcggc
     8521 tgccgattat tacgtgatca tggcagatgc cggaccgggt ggttcgattg ttccggctga
     8581 tgaagttctt gtcccagcag ggggcaccca gatttttacc atcattccaa atgccaatta
     8641 cgagatttca gatgtggttg tcaacggagt ctctctcggt ccaaggtctg tatatacgtt
     8701 ttacaatgtg aattccgacc acgtcatcag tgcacgattc tctcccctta cgggctctct
     8761 ctcgatcagt tcagatccgg caggtgcgag tgtatatatc gacagccagt atgtgggaag
     8821 gaccaaggcg cgggatgctc tgatcataga caacatcccg gtcggcaccc ggacggttga
     8881 actcatatta aaagggtaca tcccatggag tacccaggtt gtagtgagca aagatgaggt
     8941 ttctgctata cccatggttg ctctgacccc gataaccccc cctcctacta cgataccgac
     9001 cactaccgtt ccgacaacca cgataccgac cactaccgtt ccaacaacca cgataccgac
     9061 cactaccgtt ccgaccacta cgataccgac cactaccgtt cnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn ntaccgacca ctaccgttcc gaccactacg ataccgacca
     9241 ctaccgttcc gaccaccacg ataccgacca ctaccgttcc aacgacctcg actacaactc
     9301 aaacgacaac aacagcctcc cctacaaaga caacaactac cgttccaacc accataccga
     9361 ctaccgttcc cggcacttct ggtacttctg ttgcaaccac taccccgtca gcttctcctt
     9421 ctcctgtcac aaacacgcca gggacagggg aagtaacgaa tgttccttct ctaaatgaaa
     9481 cgaatatccc catgccagca tccaatcaga caactgctcc agtcacgatt gagataaatg
     9541 gaaccggcag gggaaacaac accgcactct ctttcttccc cccgattccc ggcaagctga
     9601 acccttttat tgtcattctt cttagcgggg ggctttgtct tgttcccctg ataacagata
     9661 ccgtcatcca acccatgcca tccgttcagt tccgagcaca gaggaaaatg actacaggat
     9721 tattctactt ggggattgtt gcaggaatta tcggagcagc cttctatact tatcattccg
     9781 gcttactgag tgacactatt cctcctttca tcgcgattct gcttcctgtt tcagcatatc
     9841 tggtgatatc agcgcttgtt cttactgtcg gaaccctcct gtcaaagtca ctgaaataca
     9901 cggtctccgg ccatctcctc atctccctta ttggggtatt tattgcagca cttgccctga
     9961 tcgccacaaa cgtggagtac cggctgccgt tacttatctt attttcctgt gcactggccg
    10021 gagctgtggt atcacgccgg gaatccagag atcttcgcgc catgcctgtt gctgaaaatc
    10081 ccgtattgcc aacagaaaca atcattgctc cgcggtctcc gacaattcca acgtcctttc
    10141 ccaaagaact cctcgaaaaa tacagtgatc cccagctcat cgggatgggg ggagttgcac
    10201 gcgtctttaa ggcaataaat aatcagaacg ggggcgaggt tgccctcaaa atacccgtcc
    10261 agttcaatga aacttcagga aagagcttca tgaaagagat caaggcatgg gaagatctct
    10321 cccatgaaaa tattgttaaa atacacgaag tcaacattct gcccgtccct tatgtcgaga
    10381 tggagtatct cggttcttcc cttgcagacc tccagaagcc tctcccgcca ggtgatgccg
    10441 cgaggataat ccgcgatatt gcaagaggcc tttcctacgc tcactcccag gggattatcc
    10501 accgggatat caagccccag aatattctca tgaccgaaga tggtatcccc cggatcaccg
    10561 attggggcat gagcaaggtg atggggacgg gcatgctgcc taccatcacc ggattctcac
    10621 tttcgtatgc ggcgcctgaa cagataaccc cgggcaagtt tggcgatact gaccagagga
    10681 cagatattta ccagttgggc gtggtttttt acgagctcct cactgcggaa ttaccgttcg
    10741 aaggggatga catctcacgg gtgagcgttc gaatcctcaa tgacacacca ccccctccct
    10801 cggtactcaa tccggatgcg aggccatttg atggtatcgt cgcaaaatgc ctcgaaaaag
    10861 atccagccaa tcgataccag tccatcaaag gactgatgga tgatatcgaa caggttctgg
    10921 ttactcagat cgatatcgaa cgatacgaga tttttgagta gtgcgcccgt tgtaattgcc
    10981 gggaaagggt ggtaagagtc aatcatgttc agaaaagaga ggttacactg ccagaaaaga
    11041 ccctctttca ttcttcatcg ggaagggcgc tcgagtcccg ggaaaaaaag cccccgtcct
    11101 ctccgttgcg caatacaagg tgttcttcca taatatgaaa aaattcacga gcaaggacgc
    11161 ccagctcaat gaaaaaaatc atgtatgact gattgagata taagacagga gacgcgtggc
    11221 aattttcaaa taataaggtc tttttccaaa tcggcactct agctctagtt cattatcctc
    11281 ggcaaggtcc atttgcggaa tgatgggagt ggcacgagag catcacaatt ggtcgtcccg
    11341 attatccctg tttttcactg caacgaggct cctcccgatt gctttaaagg cattctcgat
    11401 atcatcataa ttggggatat gatggttacg gagtactcca agaccgcctt tcatcgattc
    11461 cccccctatc atgcatccaa tcaccacatt ctgggtatga gcggagaatc gtgtaatctc
    11521 gagggcgagc tgggttggat cgagcactgc ggaagggacg gcgatgatga tggcgatatc
    11581 ccattcattc tgaaacctga tcagaatatc aaagacccgg gcgaagcggt ccgctcctcc
    11641 atctccaatg atatcaagag gattatccct gttccataat ctgggaagaa accggtccag
    11701 ttcctggaga agaactggag atggtgaagg gagcatgact ccaaaccttt ctgcataatc
    11761 ggatgccaga acgccaaagc cccctgcggt agagatcacg acagcacggt tccctttcgg
    11821 ataaccttca gacaccagca gttccgctac atcaaatgcc tcccgcaacg aaaagactgg
    11881 tattgccccg gcctgggtca acgctgcctg atacacttca taatctccgg cgagagatcc
    11941 ggtatgggaa gcggcggctt ttttcccatg tgtcgaagaa cctgacttga gcgtgatcac
    12001 cgggattgac tttgttgtct ccttcagttc atggagaaat gacaccccct cttttatctc
    12061 ctcaatatac aggatgattg cccttgtttc aggttcggct gctgcaaacc tgatgaaatc
    12121 aataaagccg agatcgacct ggtttccaac actgaatatc acagaaaagc caatctcttc
    12181 cgggacactc cagtcaacaa tggtcgtaat gaccgctcca ctttgtgaaa taaatccaat
    12241 atttcccttc cggggggtgg tcgggtcaaa cgtggtattg atcttgaggt ggggtagcac
    12301 aataccgagg cagtttggtc caacaatacg catacccgct ttccgggcgg tatccctgat
    12361 cttctcttcc atccgtcttc cttcatcccc cagttcccgg aaaccggatg agatgatgat
    12421 ggcaagcttg gtaccatgta cagccagctc ctcaataagg ccaggtacag cgatagaagg
    12481 aacggcaatg acggcagcat caacttcgtc agggatatcg gaaacgcttc gatatgcagg
    12541 atgcccgagt acttccccta ggttcggatt cacggggaag agcttcccgg gaaacggaag
    12601 gagatttctg aataccgcat acccgatctt ctttggatct gctgaggcac caatgacagc
    12661 aatcgatcgg ggatggaaca gatccgggtc ggagtttgtc ttctccatga tagaagaatc
    12721 cctgccgctt tcccggtata ttcgggcatc gactgcacag gccccctgtt catagaggat
    12781 cagcggattg atatcgaatt cactgatctg cccgttcgca aacatcctcg cggcattttc
    12841 cagacagcgg atcagttctt cttcatcaag agggggtttt ccccggtatc cgctgataag
    12901 ggggtaactc cggagctgcc ggatcatatc agtgagatga gctctgccca ccgggagcac
    12961 ccggagtgaa aagtccctga atagttcaat agtcgtcccc ccaagtccac aggagaggac
    13021 gcggccaaag gcaggatcgg tcttacctcc tataaaaagt tcaaggcctt ttgacacttc
    13081 tttctcaaca atgataccgg aaatgggcgc gtccgggagg tgagcgctca ccttcctgaa
    13141 gatgccattg aatgcctcct caagtccttc ttccgtattg attccggtga ccacccctcc
    13201 ggcgtcgctt ttatggacaa ttgacggaga gacaaccttg agcacgaccg gatatcctat
    13261 ccgagcagct gccgcccgtg cctcctggat gttttgagtt acggcgtgat caggtacggg
    13321 aatgccgaat tcctgaagga gtcgatatcc ttcatcctct gagagcatat gtttctgtgc
    13381 catccatcca ccagatccag aaggaggagt gggccagtca gcccataatg gttccttatt
    13441 catgcaggta ctcttcccaa aagataatcc ccggtcaatc ctatcttttt gcatgaccgg
    13501 ggagggagtt ttggaaaaat ttgaatccta tgcaaggttc catcacgagt tctccctccg
    13561 tgaattcaga aacagatgtt gtccggaaga agagcttagc cctcttttag tagcactgct
    13621 ccccacgctc tcccgtcttt cctttggatg gcgtggaacc ggtcgcgatt attgggtatt
    13681 cggaatttcc ccccgaaaac cactcattct ctcccctccc tctgccttgg cattgaccgg
    13741 acaaatcagg cagggacttg aatttgaaag actccaggac cttacgcagc actatatcga
    13801 gaataagaca ggaaaatcct gggatgatcc actggtcctt cagaggatca ggaccggcat
    13861 tatgcaacag aaaggccagt actggagcga aaaggattca cggaggattg ggtaccggaa
    13921 agggtatgcg gtccttgcat acctggctta ccatgcgccg atatatttca cacagttcca
    13981 gtatgcattc ctgatgctta taaaagacgg gcttatccca cataatgcaa gaatccttga
    14041 tatcggttca ggacccgggg tagtatccct tgccatttcc gccatctccc gtgcactacc
    14101 agaattttct gctgaactct ttgctcttga acgatccgaa gagtttcatg aatgctaccg
    14161 gtactttatt tctcagttcc caggcaaaga atccgggatc actgcatatc cggtgataaa
    14221 cggggatctt cgggatatca agacgctcct gctcccctct tccgttgatc tgattgtgct
    14281 tcagaacgtg accaatgagc tggtgcagga gactcccgaa cagaggtcgg aatacctgaa
    14341 acaagcttcc aggatcctcg caccgaatgg atgcatgatc attatcgagc cagccgaact
    14401 gagaaactcc actgagctca ggaagatggt acgcgctgca accggtcctg a
//
LOCUS       DAOOCO010000026        14421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3815_length_14421_cov_5.288668, whole genome shotgun sequence.
ACCESSION   DAOOCO010000026 DAOOCO010000000
VERSION     DAOOCO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_14421_cov_5.288668"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1051)
                     /locus_tag="PK154_02705"
     CDS             complement(125..1051)
                     /locus_tag="PK154_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPW10005.1"
                     /translation="MRPKRPDPGSNPFCPYGMERIRKGFMSLKKEVRGAQAGDDATHI
                     HRMRVASRRLRAALPLFSSCLPKKAYRRWFRAVKTITRSLGRARDLDVQIAFLGEYMR
                     NGYPSGCTKPPFQEENHSDSGFSALMRTLTSQRAAIQPEIDAIARLFSDTGVFSDLPS
                     AIRTATTVRTSRTSIRKRAARETTRRYAALIAFDPSARDPEAVKAHHAMRIAAKKYRY
                     TLEIFDEACGGRYKETIKVVRKLQEILGMMHDCDVWIETLEGAWLSREGDFGTKPGDP
                     GLIALLEDRKNERNARYRKFLLLWERFLQQSS"
     gene            complement(1032..3203)
                     /gene="ppk1"
                     /locus_tag="PK154_02710"
     CDS             complement(1032..3203)
                     /gene="ppk1"
                     /locus_tag="PK154_02710"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPW10006.1"
                     /translation="MAGGTGRQEKTDDSGKTGDFAKGRSYPSSLFINRELSWLEFNRR
                     VLSEACAPVHPLIERIKFVSIFSNNLDEFFMIRVAGLQRQLERGVREPPPDGMSPQDQ
                     LAEIHRILEPMLAQQSDLWGKELIPALQEAGIHIVSCGDLPPDDSAALRDYFQREVFP
                     VLTPLALDPSHPFPFISNLSLNLAVILNYSPRGKDNFARVKVPTDLFPRLIRVPDQAR
                     ERVPKDGIRMVFLEDLIAENLDLLFPGCIVRGAYPFRITRDADLEIEEDEASDLLTAV
                     EESIGMRRTGSPVRIEITPEMPASASGIIGRKLGINPGMFYRVSAPLGMADLMELTGL
                     DRPDLKDPPFIPAVPHGLGENDGIFSHIRDRDELLLHPYESFTPVITLLRQAATDPAV
                     LAIKITLYRVGKNSPIVDALLEARENGKVVAALIELKARFDEENNIGWARALERAGVH
                     VVYGLPGLKVHAKICMIVRREQDGLRTYVHLSTGNYNAGSARVYTDLGMFTADPAVGA
                     DAADLFNSLTGGATLLEYRRLLVAPHALRQGILSRIERETERHREHGDGCIIFKMNAL
                     VDRDCIVALYRASEAGVRVMLQVRGICCLRPGISGVSETITVTSLVGRFLEHARLYYF
                     RNGGEEEVLLGSADLMPRNLDRRVEILFPVRDRGLAAWLVKTIIPVHLGDNVKNRVLH
                     PDGTYTPLLPEPEKPLLNAQEWCLITRNGWEEEKRNAAKKT"
     gene            3528..3929
                     /locus_tag="PK154_02715"
     CDS             3528..3929
                     /locus_tag="PK154_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10007.1"
                     /translation="MHEFVRKELKKLYPSVDGWQIRPAAKSGGKEQGFIVARRILGKS
                     EGAHVLVSFDHVVTPATIDSLTAMAQNNPVPGLASPKKILVIPQNTNVMVVPSEVTVL
                     PMQSFAWENKELVWLKRRNQVSEKASAAKRS"
     gene            complement(3957..4451)
                     /locus_tag="PK154_02720"
     CDS             complement(3957..4451)
                     /locus_tag="PK154_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPW10008.1"
                     /translation="MSTERPDRPDPPSFLVDRMLGTLCRYLRFMGYDAESASSMAEGN
                     PREDTLLIRSAERKGRILLTRDRELARRAGEGAVLVAGDDVIDQLRALVNLGIIEPEL
                     RLNRCSICNELLRPAQIGEVLSAAYAPKNRQDLRFFWCRRCRKLYWLGSHREQLEKRI
                     REGL"
     gene            complement(4432..5451)
                     /gene="amrS"
                     /locus_tag="PK154_02725"
     CDS             complement(4432..5451)
                     /gene="amrS"
                     /locus_tag="PK154_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPW10009.1"
                     /translation="MENWHEARQYRKGDDLEVVCSLCSHRCHINDGKHGICGVRVNRN
                     GVLYATTFGRVSAEAIDPIEKKPLYHYLPGTLSYSLGSVGCNFRCEHCQNWHISRQGY
                     ETILADLPPKEGVQRALSAQCASISWTYNEPTIWHEYPLEMGLLAKQQGLGTVYVTNG
                     YITEEALREIRPVLDAFRVDIKAFSDAFYRKVCGAKLQPVLDAAALARELGMHIEVVT
                     LVIPGLNDSPDETGALIRWIIEYLGPDTPVHFTRYHPDYQMREPGPTPLAVLERIYRQ
                     ARELGLHFPYLGNVFPHRFENTYCPVCGALLIERQGFASAFRNLSGKQCTQCGAVIPY
                     VDRTA"
     gene            complement(5516..5662)
                     /locus_tag="PK154_02730"
     CDS             complement(5516..5662)
                     /locus_tag="PK154_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10010.1"
                     /translation="MIRCPVCRSIEIYRVAGGCIGEVYRCKQCGYYGSFVVEEDEPPR
                     NNGE"
     gene            5705..6418
                     /gene="pyrH"
                     /locus_tag="PK154_02735"
     CDS             5705..6418
                     /gene="pyrH"
                     /locus_tag="PK154_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPW10011.1"
                     /translation="MKKVVLSLGGSVLIPALEEHRIARYATVLQEIAGYVRLYVVVGG
                     GGEARRYITAAREIGCSEALADEIGVAVTRLNAMLLAGALGEAACPLIAESQAEAMAY
                     GAGDKIVLMGGVTPAQTTDAVAAVLAERCGADLMVNLTSVDGIYTADPRTDPTAERLD
                     QLTPERLLEIVGTGSLKAGANTVIDMIAAKVIQRSGIPLLVIDGRDPGNLSRALLSGT
                     YSGSLVTRGGERPLPVRGT"
     gene            6471..6545
                     /locus_tag="PK154_02740"
     tRNA            6471..6545
                     /locus_tag="PK154_02740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6507..6509,aa:Pro,seq:tgg)
     gene            6599..7249
                     /gene="nth"
                     /locus_tag="PK154_02745"
     CDS             6599..7249
                     /gene="nth"
                     /locus_tag="PK154_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPW10012.1"
                     /translation="MDRKNARRILAILAGRYPDRWARERYAASPFEVLITTILSAQTT
                     DKSVDAIRDELFSRFPGPQSLATADIAEVEEIIHSTGFFHMKAKHIIAASRTLVDKFS
                     GVVPDTMEDLLTIPGVGRKTANIVLYHAFGKNEGVAVDTHVFRLAERLGFSDSRNADG
                     IERDLMRLFPEKDWGVLTDILISHGRAVCMARKPACPVCPVSPLCKYYLEKERGKA"
     gene            complement(7358..8392)
                     /locus_tag="PK154_02750"
     CDS             complement(7358..8392)
                     /locus_tag="PK154_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW10013.1"
                     /translation="MLVPTPDIIAIIILIALIFDFTNGFHDSANSISTVISTKVLSPQ
                     RAVVFAAFFNFIAAFFFGVAVASTISKIINLGYVTPVIVPYIILAALFGAIGWNLITW
                     YSGLPTSSSHALIGALAGAGISAGGLAAIKMGTIDTVILFMVLSPIIGFIVAFLFMSC
                     VLWISKGAQRMVAERHFRRLQLFSAAAYSFSHGTNDAQKTIGIITPLLFSIGYFGTAV
                     DPNNLPVPIWVILAAYSAIALGTLTGGWRIVKTMGYNITRLRPVDGFAAETAGAATII
                     GASIAGIPVSTTHVISSSIMGVGATQGSGYVRWSVARRIVWAWVLTIPVSALMGYLFF
                     SGIFYIVNLF"
     gene            complement(8476..9105)
                     /locus_tag="PK154_02755"
     CDS             complement(8476..9105)
                     /locus_tag="PK154_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW10014.1"
                     /translation="MGLKEWIIPQDDIFFDHFDRLSAVLVLASYRLVDLTDNFGDLKN
                     YAQQIKQLEHEGDKITHEIYEQLNISFITPLQPDEISHLASSLDDVLDYIDSTTRKMD
                     YYEITEVDSYMRELIRLIQLSVIEIEQAVKCIRSIKDPMQIEKRCIEVNRLENVADDV
                     LAHAIKDLFKTKGAVDIIKYKDIYEYLEIATDKCEDVANILSDLAIRHS"
     gene            complement(9205..9276)
                     /locus_tag="PK154_02760"
     tRNA            complement(9205..9276)
                     /locus_tag="PK154_02760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9242..9244),aa:Ala,seq:ggc)
     gene            complement(9312..10490)
                     /locus_tag="PK154_02765"
     CDS             complement(9312..10490)
                     /locus_tag="PK154_02765"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPW10015.1"
                     /translation="MGRFAIVDDDAIRRGQCTDIYFVRTEEILRKSGKNPRVAMEVTA
                     SSLPDGFAVFCGLDDVITLLEGIPICLDAMDEGSIFYPGEPVLRIEGHYQDFIRYETA
                     ILGFLCHASGIATAAASLRCISGGRAIYSFGSRRQHPAIATMVERAAWVGGVDAVSNT
                     TAPPEIPLSGTMPHSFVMCYSSPNEAFIAFDRYADPDVPRIFLCDTFCDEKMESLAGA
                     RSGAQGVRLDTPRSRRGNMRAIIEEVRWELDAAGYREVKMVLSGGVTRDDIIAYRDLV
                     DAFGVGGTIANAPVVDFALDIVSIEGNPSAKRGKKSGEKQVWELPDGRHHLLPAGQPG
                     PGEGIPLLSPYLKEGVVVQRPPLSAARERLMRSLMQRGWLDSSGVDCAGAPTDPARDK
                     "
     gene            10609..11280
                     /locus_tag="PK154_02770"
     CDS             10609..11280
                     /locus_tag="PK154_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPW10016.1"
                     /translation="MLGIIGGTSLLFTRLPDLEPRVVYTPFGNSEVLCGEYALLMRHQ
                     RNKPPHRINFRSHLAALALCGVTHLVSIGSAGTLKQEIPEGSVVIPSDYMSMAPIPSF
                     HDHAIHHVLPEFSPGLSRQLAALIPEARHGGIYVQTPGPRLETRAEVRALAASADLVG
                     MTVASEATLARELGMEIAALCTVDNYAHGIADESPTYEHLLKRSEECKDRTGDMVRRI
                     IAGLA"
     gene            11277..12602
                     /locus_tag="PK154_02775"
     CDS             11277..12602
                     /locus_tag="PK154_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW10017.1"
                     /translation="MMGVPPEGFPDKPGSLLIRGALTERGPVDVCISREGFIVAAGPD
                     AGRTFRGEADSVIEGTGRVLLPGLVNTHTHAAMTLLRGYADDMPLQQWLSEKIWPLES
                     HLTGDDVYWGTRLACLEMIKTGTTAFHDMYFFMEDAARAVEAAGIRAVLTYGFIDLFS
                     EERREQEIRATERFVGHIKALGNPHIKASVGPHAVYTVSQEGLRWLAEYSREEGIGIH
                     IHLSETEQEVGDAVKAWGMRPPGILDRCGILTPRTVAAHCCWLDQAECTLLGERGVSV
                     AYNPASNMKLAVNRAMPYHWLREAGVNVSLGTDGCSSNNNLDMFEEMKTGALLQKFYW
                     NSPTLLPATEMLAMATSGGARALGIGSGTITPGTPADLLLLDRHAPCNTPMHQIPSNA
                     VYSCNGSAVTTVICQGRVLMLDRYIPGEEEVISRASAVAADLVKRSGTA"
     gene            complement(12706..14406)
                     /locus_tag="PK154_02780"
     CDS             complement(12706..14406)
                     /locus_tag="PK154_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW10018.1"
                     /translation="MDPRSYDCGISTQPLLGLTLGEMLSSVAARHPDTEAVVSVHQDI
                     RWTYRELLSQVDRLAKGLMALGVEKGDRVGIWAMNYAEWIAVQFATATIGAIMVNINP
                     AYRTYELEYVLKQAEIQTLILQGRFKTSDYIGMFYEACPDAQEARPGRLNSEKFPFLK
                     NVVFLGDIAYNGMYTWPDLMKKGEKISDDELIARGEMLDFDDPINIQYTSGTTGFPKG
                     VVLTHHSVLNNGYIIGEGMGFTEKDRLCIPVPFYHCFGMVLSNLACMTHGSTMVIPSP
                     TFSAEDVLKTVEQEQCTALHGVPTMFIAELSHPDFPNYSMKSLRTGIMAGSPCPIEVM
                     KEVNRKMNMSEIVIVYGQTETSPGVTMTTTRDPVERRVSTVGRVFPHTELKIVDPKSG
                     KIVPRGEIGEICARGYCVMKCYYNNPSATHATLDTNGWNHTGDLGTMDEEGYVKIVGR
                     LKDMVIRGGENIYPREIEEFLHHHPKIADVYVIGVPDEKYGEELMAWVMLTEEAGTTE
                     EEIKEFCRGKIAHYKIPRYVKFVKEFPMSVSGKIQKFKMRELSIRELQLEDASRIETA
                     "
BASE COUNT         3034 a         4186 c         4229 g         2972 t
ORIGIN      
        1 ctggaggtaa ggaacatgaa accttttaag aacggctatt ctttgaaagg agccttccag
       61 gggggcatat ctgccggttg ctgacacttc cggacttttt ttcagcctca ccgtgctccc
      121 ctgttcagga agactgctgc aggaatcgct cccagagcag gaggaacttc cggtatcggg
      181 cattccgctc gttcttcctg tcttcaagca gggcgatcag ccccgggtca cctggctttg
      241 ttccgaaatc tccttccctg gaaagccacg ctccttccag tgtttcgatc cagacatcgc
      301 agtcatgcat catcccgagg atctcctgga gcttccgcac caccttgatc gtttccttgt
      361 acctgccgcc acatgcctcg tcaaagatct ccagggtgta ccggtatttt tttgctgcta
      421 tccgcatcgc atgatgggct tttacagcct ccggatccct ggcagaagga tcgaacgcga
      481 taagtgctgc ataccgcctc gttgtctcac gggctgcccg tttacggatg ctggtgcggg
      541 atgtgcgcac ggtagtggca gtacgaattg ctgacgggag gtcagaaaag acacccgtat
      601 ccgagaagag acgtgcaatt gcatcgatct cgggctggat ggctgcccgc tgtgacgtga
      661 gtgttctcat gagcgctgaa aatccggaat cggagtgatt ctcttcctgg aatggtggtt
      721 ttgtgcatcc agaaggatac ccgttcctca tgtattcccc gagaaatgca atctggacat
      781 ccagatcgcg ggcccttcca agggatctcg tgatcgtctt cacggcacga aaccacctgc
      841 gataggcctt tttcgggagg caggaggaga agagtggcag tgcagcacgc aggcggcgtg
      901 aggcaaccct catccggtgg atatgggtgg catcgtcccc ggcctgtgcc cctctcacct
      961 cctttttcag ggacataaat cccttcctga tacgctccat cccatagggg cagaagggat
     1021 tgctgcctgg atcaggtctt tttggccgca ttcctcttct cttcctccca tccgtttctg
     1081 gtgatcaggc accattcctg ggcattcagg agcggctttt ccggttcggg cagcaggggg
     1141 gtataggttc catccggatg aagcacccgg ttcttgacat tatcgccaag atgcacagga
     1201 atgatcgtct tcaccagcca tgccgcaagg ccccggtccc tgaccgggaa aaggatctca
     1261 accctccggt caagattccg tggcatcagg tccgctgatc cgagcagcac ctcctcctcc
     1321 cccccattcc ggaaataata caggcgggca tgttcaagga acctgcccac gagcgaggtg
     1381 acggtgatcg tttcactcac cccgctgatg ccaggccgca ggcagcagat gccccggacc
     1441 tggagcatga cccggactcc tgcctctgaa gcccggtaga gggcgactat acagtcccgg
     1501 tcaacgagag cattcatctt gaatatgatg catccgtcac catgctcgcg gtgacgctcg
     1561 gtctcccgct cgatccggga gaggatcccc tggcggaggg catgaggcgc aacgagcagt
     1621 ctccggtatt ccagcagtgt tgcacccccg gtcaggctat tgaacaggtc agcagcatcg
     1681 gcaccgacag cgggatcggc ggtgaacatg ccgaggtccg tgtacaccct ggcagatccg
     1741 gcgttataat tcccggtgct caggtggaca taggtacgga ggccgtcctg ctcacgccgt
     1801 acgatcatgc agatcttggc atgcactttg agcccgggaa ggccatagac gacatgtacc
     1861 cctgctctct caagtgcccg tgcccaaccg atattgttct cctcgtcaaa ccgggccttg
     1921 agctcgatca aggctgccac caccttgcca ttctcccggg cttcaagaag ggcatcgacg
     1981 atcggtgaat ttttcccgac ccggtagagc gtgatcttga tggcgagcac ggccggatca
     2041 gtggctgcct gacggagcag agtgatgacc ggggtgaagc tctcgtaggg gtggagcagg
     2101 agctcatccc tgtcgcggat atgggagaat atcccgtcat tctccccaag gccatgcggg
     2161 actgccggga tgaagggggg atccttcagg tcaggacgat ccagcccggt cagctccatc
     2221 aggtctgcca ttccaagggg agcgctcacc cggtaaaaca tccccggatt gatcccaagc
     2281 ttcctcccga tgatcccgct ggcagaggcg ggcatttcag gggtgatctc aatccggacc
     2341 ggggaccctg tcctccgcat gccgatactc tcttcgaccg cagtgagaag atctgatgcc
     2401 tcgtcctcct cgatctcgag gtccgcatca cgggtaatcc tgaagggata cgctcccctg
     2461 acgatgcagc cggggaagag cagatcgagg ttttctgcga tgagatcctc gagaaacacc
     2521 atccgtatcc cgtctttcgg aacacgttcc cgggcctggt cagggacccg gatcaaacgg
     2581 ggaaagaggt cggtggggac cttgacccgg gcaaagttat cctttcctcg cggggagtag
     2641 ttcagaatga ctgccaggtt gagtgaaagg ttcgagataa aggggaaggg atgggatggg
     2701 tccagggcaa ggggcgtgag gacgggaaat acttcacgct ggaaatagtc gcggagcgct
     2761 gcactgtcat ccggggggag gtccccgcag gagacgatgt ggatccctgc ctcctgcaga
     2821 gccgggatga gttcctttcc ccagaggtcg gactgctgtg caagcattgg ttcaaggatc
     2881 cggtggatct ctgcgagctg gtcctggggg ctcatgccat caggaggagg ttcacgcacc
     2941 cccctttcca gctgccgctg cagtcctgcg accctgatca tgaagaattc atcgaggttg
     3001 ttggagaaga tggagacgaa cttgatccgt tcgatgagag ggtggacagg ggcacaggct
     3061 tccgaaagga ccctccggtt gaattcgagc caggagagtt cgcggttgat gaagagagag
     3121 gacggataag accggccttt cgcgaaatca cccgtcttcc cggaatcatc cgtcttttcc
     3181 tgtctgcctg ttccgcccgc catcctcctc accgggtttc acatactatt agagaaggaa
     3241 aatttaaaac catgcgggag agcagggaga ggacttcccg cccgggcagg acgggcgcga
     3301 atgctctctt cccgggacca ggtcctcccc ccgggttcat ccgctatttc tcccttttca
     3361 ccctgccggt gcggataccg cagatagtgc ggagggcgga gtgggggaac aatccctgca
     3421 ttccgatggc tgaaaaatgc ccggccgcca ccataccaaa caattttagt agttatctga
     3481 caatctatct tctgccgtga ttgaccatgg gatctaatga cgacatcatg catgaatttg
     3541 taagaaagga actcaagaaa ctctatccca gcgttgacgg atggcagatc aggccagcgg
     3601 caaaatccgg aggaaaagag cagggattca tagtggcaag gagaatcctt gggaaatcag
     3661 agggtgccca tgtgctggtc tcattcgacc atgtcgtgac acccgctacc attgattccc
     3721 ttacggccat ggcacagaac aaccctgtcc cgggtcttgc atccccgaaa aagatcctcg
     3781 tcatcccgca gaacaccaat gtgatggttg ttccttcaga ggttactgtc ctcccgatgc
     3841 agagctttgc atgggagaac aaggagctcg tctggctcaa gagaagaaac caggtgagcg
     3901 agaaggcctc cgctgcaaag cgttcctgaa aaaaggaaag cagaccgaca agacgactaa
     3961 agaccctccc ttattctttt ttcgagctgc tcgcggtggg aacccagcca gtagagtttc
     4021 ctgcatcgcc tgcaccagaa aaaccggaga tcctgtctgt ttttcggggc gtaagcagcg
     4081 gacaaaacct ccccgatctg tgcaggccgg aggagttcgt tgcagatcga acaccggttc
     4141 agccggagct ccggctcgat gatgcccagg ttcacgagcg ctctgagctg gtcgatgaca
     4201 tcgtctccag cgacaaggac tgccccttcc cctgcacgcc tcgcaagctc cctgtccctg
     4261 gtcagcagga tccgcccctt ccgctccgca gaccggatca ggagcgtatc ctcccgggga
     4321 tttccctctg ccatggaact ggcactctcg gcatcgtatc ccatgaaccg caggtagcga
     4381 cagagggtgc caagcatcct gtccaccagg aatgaggggg gatccgggcg gtcaggccgt
     4441 tctgtcgaca taagggatca ctgcaccgca ttgggtgcac tgctttcctg acaggttcct
     4501 gaaggcagat gcgaatccct gccgctcgat caggagtgcc ccgcagaccg ggcagtacgt
     4561 gttctcgaac cggtgcggga agacattgcc aaggtaggga aaatggagcc cgagttccct
     4621 ggcctgccgg tagatcctct caagaacggc gagcggggtc ggtcccggct cacgcatctg
     4681 gtagtccggg tgatacctgg tgaagtggac aggggtgtcc ggtcccaggt attcgatgat
     4741 ccatcggatg agagccccgg tctcatcggg tgaatcgttc agcccgggga tgacaagggt
     4801 gaccacctcg atgtgcatgc caagctcccg ggcaagcgcg gccgcatcga gaaccggctg
     4861 gagtttggcc ccgcagacct tccggtagaa tgcatcggaa aaggccttga tgtccaccct
     4921 gaacgcatcg agaacgggcc tgatctccct gagtgcctcc tcggtgatat acccgtttgt
     4981 cacgtatacc gtcccgaggc cctgctgttt tgcgaggagt cccatctcaa ggggatattc
     5041 atgccagatg gtcggttcgt tgtaggtcca ggagatgctt gcgcactggg cagagagggc
     5101 tctttgcacc ccttccttgg ggggcagatc ggcaaggatc gtctcgtatc cctgccgcga
     5161 gatgtgccag ttctggcaat gctcacaccg gaaattgcac ccgacgctgc cgagtgaata
     5221 cgagagcgtc ccgggcaggt aatggtacag gggtttcttc tcgatgggat cgatcgcctc
     5281 cgcactcacc ctgccaaacg tggttgcgta gaggaccccg ttccggttca cccggacccc
     5341 gcagatcccg tgcttcccgt cattgatatg gcaccggtgg ctgcagaggg agcagaccac
     5401 ctcgagatcg tcccctttcc ggtactgccg cgcttcgtgc cagttttcca tcagaggaga
     5461 taaccgggga ggtacaagat aaacataccg gtgtcaggag gccctttatc gctgatcact
     5521 ccccgttgtt ccggggaggt tcatcctcct ctacgacaaa ggagccatag tagccgcact
     5581 gtttgcaccg gtatacctca ccgatacacc cgccggctac cctgtagatc tcgatactcc
     5641 tgcagaccgg gcagcggatc atcttcaaca attatatgta gcgtataaag aaaccattgg
     5701 gtgcatgaag aaggtcgttc tctcgctggg cggatccgtg ctcatccctg cccttgaaga
     5761 gcaccggata gcccgctacg ctacggtgct gcaggagatc gccgggtatg tccggttgta
     5821 tgtggtggtc ggaggcggcg gggaggcccg gagatacatc acggcagcac gggagatcgg
     5881 ctgctccgag gccctggctg atgagatcgg ggttgcggtc accaggctga atgcgatgct
     5941 gcttgccggt gcgctcggtg aggcggcctg ccccctcatt gcggaaagcc aggccgaggc
     6001 gatggcctac ggggcaggag acaagatcgt cctgatgggc ggggtcaccc ctgcccagac
     6061 cacggatgcg gtggctgcag tcctagcaga gcggtgcggg gcagacctga tggtcaacct
     6121 cacttcggtg gacggcattt acaccgcgga tcccaggacc gatccgacag ccgaacggct
     6181 cgaccagctc actcccgaac ggctgctgga gatcgtgggg accggatcgc tcaaggcagg
     6241 ggcgaacacg gtgatcgata tgatcgccgc aaaggtgatc cagcgctcgg gcatcccgct
     6301 gctcgtcatt gatggccgcg acccgggcaa cctgtcgcgt gcgctccttt caggcaccta
     6361 ttccgggagc ctggtcacca ggggtggcga gaggccgctg cctgtcaggg gaacctgaac
     6421 gggacaggta actatttttg ggtgtctgac caaccatcct cctcatatca ggggtagtag
     6481 ggtagcctgg tccatcctag agcgtttggg acgctttgac ggcagttcga atctgcccta
     6541 ccccatctcc ttctctcaat cgtgcccatt ttatggatca cggcaccacc tgttctgcat
     6601 ggacaggaag aacgcccgcc ggatccttgc catccttgcc ggccggtacc cggaccggtg
     6661 ggcccgggaa cggtatgcag catccccctt tgaggtgctg atcacgacca tcctttctgc
     6721 acagaccacg gacaagtcgg tggatgccat cagggatgag ctcttctccc ggtttcccgg
     6781 cccgcaatcc ctggcaaccg ccgatatcgc tgaggttgag gagatcattc atagcaccgg
     6841 gttcttccac atgaaggcca aacacatcat tgccgcttca cgcaccctcg tcgataagtt
     6901 ctccggggtg gtccctgata ccatggagga tctcctgacc attcccgggg tggggaggaa
     6961 aacggcgaat atcgtgctct accatgcctt cggaaagaac gaaggggtgg cggtggacac
     7021 ccacgtgttc aggctcgccg aaaggcttgg gttttccgac tcccggaatg ctgacggtat
     7081 tgagagggac ctgatgcgcc tgttcccgga gaaggactgg ggggtcctca ccgacatcct
     7141 catctcgcat ggcagggcgg tctgtatggc cagaaaaccg gcctgcccgg tctgcccggt
     7201 ctcccccctc tgcaagtact acctggaaaa ggagagagga aaagcatgag agaatcagcc
     7261 ttgaactgat acaagcgtca agggaagact gacatctcac aggcggcgat ggggggggcg
     7321 atcaccaggg ggcctttgct gttttctatt cacaatatca gaacaggttc acgatataaa
     7381 agataccgga gaagaacagg tagcccatga gtgcgcttac cggaatggtg aggacccatg
     7441 cccacacaat ccgccgggca acgctccacc gtacataccc gcttccctgc gttgcaccaa
     7501 cacccatgat cgatgaggag atgacatggg tggtgctgac agggattcct gcaatggagg
     7561 ctccgatgat ggttgctgcc ccagctgttt cagcagcgaa tccatccacg ggtctcaagc
     7621 gggtgatatt gtagcccatt gtcttgacaa tacgccatcc tcccgtgaga gtgcccaatg
     7681 ctatggcact gtaggcagcc agaatgaccc atatcgggac agggaggttg tttggatcca
     7741 ctgccgtgcc aaaatatccg atactgaaaa ggagcggggt gatgatacca attgttttct
     7801 gggcgtcgtt ggtcccgtgg ctgaaactgt aggcagcggc tgagaagagc tgcagtctcc
     7861 tgaaatggcg ttcagcaacc atccgctggg caccttttga tatccagagg acgcacgaca
     7921 tgaacaagaa tgccacgatg aacccgatga tgggagagag taccatgaag aggattacgg
     7981 tgtcgatggt tcccatcttg atcgccgcca atccgcccgc agagatacct gccccagcga
     8041 gagccccgat cagcgcgtga gaagaggagg tcggaagacc ggaataccag gtgatcaggt
     8101 tccaccctat cgcaccaaag agggcggcaa ggatgatata cgggacgatc acgggggtaa
     8161 catacccgag attgatgatc ttgctgatgg tgcttgcgac cgcaaccccg aagaagaagg
     8221 ccgcgatgaa gttaaaaaaa gcggcgaaca ccaccgccct ctgcggggaa agaaccttcg
     8281 tggagatgac ggttgatatc gagttggccg agtcatggaa tccgttcgta aaatcgaata
     8341 tcagggcgat gaggatgatg atcgcgataa tatccggagt cgggaccagc atggatcagc
     8401 acgctcccgg caccacagga ataagaacca caccggcaat tcgtcccatg gctttcatcg
     8461 gcaggtgcca cctcgtcagg aatgccggat tgcaagatcg gaaaggatgt ttgccacgtc
     8521 ctcgcacttg tcggtcgcaa tctccaggta ttcgtagata tccttgtact tgatgatgtc
     8581 gacggcaccc tttgtcttga agagatcttt tatcgcatgg gcaaggacgt catcggcgac
     8641 gttctcaagc cggttcacct cgatgcagcg cttctcgatc tgcatcgggt ccttgatcga
     8701 ccggatgcac ttgaccgcct gctcgatctc aatcaccgag agctggatca gcctgatcag
     8761 ctcccgcatg tacgaatcca cctcggtgat ctcgtagtag tccatcttcc ttgtcgtcga
     8821 atcgatgtaa tcgagcacgt catcgagcga tgaagcaaga tgggagatct catcgggctg
     8881 gaggggggtt atgaaactga tgttcagctg ctcgtagatc tcgtgggtga tcttgtcacc
     8941 ttcatgctcg agctgcttga tctgctgggc ataattcttc aggtccccga aattgtcggt
     9001 gagatccaca agccggtagg acgcaagcac caggacggca gataaacggt cgaaatggtc
     9061 aaaaaagatg tcatcctgcg gtattatcca ttccttgagg cccacgagtg atcgatgcta
     9121 catcatccca cagaggtaaa aaaactccga tctgaatttg ataaggctgc ccgggaccga
     9181 gcagcgcaaa cgaacaaaat gtgatggacc gagcgggaat tgaacccgcg gcctcttcca
     9241 tgccaaggaa gcgatcttcc tctgatctat cggcccgctc ctagtaatgt ggtcgctcct
     9301 ggttaaaaga attatttatc gcgagccggg tcggttggag cgccagcgca atccactcca
     9361 gaggagtcca gccatccccg ctgcatcagc gaacgcatga gccgttcccg tgctgcagag
     9421 agcggggggc gttgcacgac cactccctcc ttcaggtaag gagagaggag cgggattccc
     9481 tcccccggcc ccggctgccc ggcggggagg aggtgatgac ggccatccgg gagctcccag
     9541 acctgtttct ccccgctctt cttgccccgc tttgcggagg gattgccctc gatcgagacg
     9601 atatccaggg caaagtcgac taccggggca ttggcaatcg ttccacccac cccgaacgca
     9661 tccacgagat cccggtaggc gatgatgtca tcgcgggtca cccccccgga aaggaccatc
     9721 ttcacttcgc ggtaccctgc agcgtcaagc tcccacctga cctcttcgat gatcgcccgc
     9781 atgttacccc tcctcgaccg gggtgtatcc agccgcactc cctgcgcccc tgatcgtgcc
     9841 cctgcaagcg attccatctt ctcgtcgcag aacgtgtcac agaggaagat ccgggggaca
     9901 tccgggtcgg cataccggtc aaaggcaatg aatgcctcgt tcggggagga gtagcacatc
     9961 acaaaggagt ggggcatcgt gccggagagc gggatctcag ggggagcggt agtattcgag
    10021 accgcgtcaa cccctcctac ccatgcggcg cgttcgacca tcgtggcgat ggccgggtgc
    10081 tgtctccggg agccaaagga gtagatcgct ctccccccgg agatacaccg gagcgaagcg
    10141 gcagcggtag ctataccgga agcatggcag aggaatccca gtatcgcagt ttcgtaccgg
    10201 atgaaatcct ggtagtgccc ttcgatcctg agcactggtt ctccaggata gaagatcgat
    10261 ccctcgtcca ttgcgtcgag gcagatcgga attccctcga gtagggtgat gacatcatcg
    10321 agcccgcaga agaccgcaaa gccgtcaggg agcgaggagg cggtcacctc catggccacc
    10381 cggggatttt taccggactt gcggaggatc tcttcggtcc tgacaaaata gatatcggtg
    10441 cattgaccgc gcctgatcgc gtcatcatca acgatggcaa acctgcccat ggagaggaga
    10501 tctggaagag ggggctgata cagcttccgg gacagccctc ctgcgaaaag accgaaaatg
    10561 atacattctg catgcatcct cactatactg acaacaggtg agtcgacgat gctaggaatt
    10621 atcgggggca caagtctcct ttttacccga ttgcctgacc ttgaaccaag ggtggtttat
    10681 accccgtttg gaaattctga agtgctttgc ggtgagtatg cactgctcat gcggcaccag
    10741 cggaacaagc ccccgcaccg gatcaatttc cggtcccatc ttgcagcgct tgccctctgc
    10801 ggggttacgc accttgtctc catcgggtcg gcagggaccc tgaagcagga gatccctgaa
    10861 ggatccgtgg tgatcccctc cgattacatg agcatggcac ccatcccctc cttccatgac
    10921 catgcgatcc atcatgtcct cccggagttc tctcccggct tgtcccggca gcttgcagcg
    10981 ctcatcccgg aagcccggca cggcgggatc tatgtgcaga ccccgggacc acgcctggag
    11041 accagggccg aggttcgtgc cctcgctgcc agtgctgacc tggtcggcat gaccgtggcg
    11101 tcagaagcca cccttgcccg ggagctcggg atggagattg cagcgctctg caccgtggac
    11161 aactatgccc atgggatcgc cgatgagagc ccgacctatg aacatctcct gaaacggtcc
    11221 gaggaatgca aggaccggac cggagacatg gtcaggcgga ttattgcggg gctggcatga
    11281 tgggagtacc acctgaaggg ttccctgata agccgggctc gctcctgatc aggggagcgc
    11341 tcacggaaag gggaccggtt gacgtctgca tcagcaggga gggattcatc gtcgccgcag
    11401 gaccggatgc cggccggacc ttcaggggcg aggcggactc cgtcatcgag ggaacaggca
    11461 gggttctcct ccccggtctt gtcaataccc atacccatgc tgccatgacc cttctccgag
    11521 gctacgccga tgacatgccc ctgcagcagt ggctctccga aaagatctgg ccgctggaat
    11581 cccatctcac cggggacgat gtctactggg gaacgcggct tgcctgcctc gagatgatca
    11641 aaaccggcac cacagcgttc catgacatgt acttcttcat ggaggacgct gcccgggcgg
    11701 tagaagcagc cgggatacgg gcagtgctga cctatgggtt catcgatctt ttcagcgagg
    11761 agagacggga gcaggagatc cgggcaaccg aacggtttgt cggtcatatc aaggccctgg
    11821 gaaatccgca cataaaggcg tcggtcgggc cgcatgccgt ctacacggtc tcacaggaag
    11881 ggctccgctg gctggcagag tacagcaggg aggaggggat cgggatccat attcaccttt
    11941 ccgagaccga acaggaggta ggggatgcgg tgaaggcgtg ggggatgcgg cctcccggga
    12001 tccttgaccg gtgcggcatc ctcaccccgc ggacggtggc cgcccactgc tgctggcttg
    12061 accaggcgga gtgcaccctc ctgggagaac ggggcgtctc tgtcgcctac aatcctgcaa
    12121 gcaacatgaa actggccgtg aaccgggcga tgccctacca ctggctccgg gaggccgggg
    12181 tgaatgtctc gctcgggacg gatgggtgtt cttcgaacaa caacctcgat atgtttgagg
    12241 agatgaaaac cggagccctc ctccagaaat tttactggaa ctccccgacc ctcctcccag
    12301 ccacagagat gctggcgatg gccaccagcg ggggagcacg ggcactcggg atcgggagcg
    12361 gcaccatcac tccgggcact cctgctgacc tgctactcct cgaccggcat gcgccctgca
    12421 acacaccgat gcaccagatc ccctccaatg cggtctattc atgcaacgga tcggcggtaa
    12481 caacggtcat ctgccagggg agagtactga tgctggaccg gtacatcccg ggggaggagg
    12541 aggtgatcag cagggcatct gccgttgctg ctgacctcgt gaagcggagc gggacggcat
    12601 aggagagaga agccctctcg tcccggttcc ggaaaaaagg cgggtatgga ccgaacctgg
    12661 aggccggcgc ccgggatggc caggcctgga ggcgggtagt ctgtttcagg ccgtctcgat
    12721 ccgggaggcg tcttcgagct gcagctcccg gattgagagt tcccgcatct tgaacttctg
    12781 gatctttcct gaaacgctca tcgggaactc tttgacgaat ttcacgtagc ggggaatctt
    12841 gtagtgggct atctttcccc ggcagaactc cttgatctcc tcctcggtgg tgccggcctc
    12901 ctccgtcagc atcacccagg ccatcagctc ttccccgtat ttttcatcgg ggaccccgat
    12961 cacgtagaca tcggcaatct tcggatggtg gtggaggaac tcctcgatct cgcggggata
    13021 gatattctcc ccgcccctga tcaccatgtc cttcagcctc ccgacaatct tgacgtagcc
    13081 ctcttcatcc atcgtcccga gatccccggt atggttccat ccgttggtat caagggtggc
    13141 atgggtagcg ctcgggttgt tgtagtagca tttcataacg cagtagcccc gggcgcagat
    13201 ctcgccgatc tcgccacgcg ggacgatctt cccgctcttc gggtcgacga tcttcagttc
    13261 ggtgtgggga aatacccttc cgacggttga gacacgccgc tcaacgggat ctctcgtggt
    13321 cgtcatggtc acaccagggg aggtctcggt ctgcccgtag acgatcacga tctcggacat
    13381 gttcatcttc cggttcacct ccttcatcac ctcgatcggg cagggagagc cggccatgat
    13441 cccggtgcgg agcgatttca ttgagtagtt cggaaagtca ggatgggaga gctcggcgat
    13501 gaacatggtc gggaccccgt gcagggccgt gcactgctcc tgttcgaccg tcttcaacac
    13561 atcctcggca ctgaaggtag gggaggggat gaccatggta gacccgtgcg tcatgcacgc
    13621 gaggtttgag aggaccatcc cgaaacaatg gtagaacggg accgggatgc agaggcggtc
    13681 tttttccgta aaacccatcc cttccccgat gatgtacccg ttgttgagga cactgtggtg
    13741 ggtgagcacg acccccttgg gaaatcctgt tgtcccgctc gtatactgga tgttgatggg
    13801 atcgtcgaaa tcgagcatct ctcccctggc aataagctcg tcatcgctga tcttctcccc
    13861 cttcttcatc aggtcaggcc aggtatacat gccattgtag gcgatatccc cgagaaagac
    13921 cacgtttttc aggaacggga atttttccga gttcagtctc ccgggcctgg cctcctgcgc
    13981 atcaggacag gcttcgtaga acatcccgat ataatccgag gttttaaacc gtccctgcag
    14041 aatcagggtc tggatctcgg cctgcttcag aacatattcc agttcatacg tccggtatgc
    14101 cgggttgata ttcaccatga tggcgccgat cgtggctgtg gcaaactgga cggcgatcca
    14161 ttcggcataa ttcattgccc agattcccac ccggtcacct ttctcgactc ccagcgccat
    14221 cagccccttt gcgaggcgat cgacctggga gaggagctcc cgataggtcc accggatatc
    14281 ctggtggacc gagactaccg cttccgtgtc aggatggcgg gcagcgacag aagagagcat
    14341 ttcaccgaga gtgagaccaa gaaggggctg ggttgagata ccgcaatcat agctccgggg
    14401 atccatccta acaaaagagg a
//
LOCUS       DAOOCO010000027        14185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_3915_length_14185_cov_4.933333, whole genome shotgun sequence.
ACCESSION   DAOOCO010000027 DAOOCO010000000
VERSION     DAOOCO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_14185_cov_4.933333"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PK154_02785"
     CDS             complement(<1..630)
                     /locus_tag="PK154_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10019.1"
                     /translation="MNKTVAFVFILCLVGALVIPAASAPVKSTINSPSASTQPLKPTV
                     PDSPGASDEDHGRVSSFRATLEENGFIVTNGQMTPVNPIVDLLDSGLGDSANGNNAGQ
                     TYKVLMVPISPFIEENPFEGGLTIFRLMPDEAVVYLGPTPPPCDYFSFTVFLWVRHKE
                     SLIPKGDWMFAAVNDPLNSALIKTQGNGTPFRKQTIVIFTADRGVYERVA"
     gene            809..1495
                     /locus_tag="PK154_02790"
     CDS             809..1495
                     /locus_tag="PK154_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HPW10020.1"
                     /translation="MLPRVILHVATSLDGRITNFPANLDLYYALAATWKPDAILFGSE
                     TVVAAVRENPSLEVPKEHEDLFTPPEGAEDTRPLLVIADSRGRVRCWDAIRTWPYMRD
                     LLAFCSAATPKEYLDYLAERRIDAIIAGEDRIDMREALSELNRRHGVKTVRVDSGGTL
                     NSVLLEAGVVAEVSVLVHPFLAGGKPDPTMFDPAKAGFPDLQVPLELIRTEVVGDGLV
                     WARYSVKQSP"
     gene            1727..2695
                     /locus_tag="PK154_02795"
     CDS             1727..2695
                     /locus_tag="PK154_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPW10021.1"
                     /translation="MGIKIGYFSVTDAMTPAGCLRQSIEAEKIGFDSLWVEDHLIAMP
                     GGKECSFAWTVMSSALQATKKVPFITGVTAPILRYHPAIVAQAFATMAAMYPGRVGLG
                     VGTGEPPNEMAVYGGEWPSNNARLEMLEEALTVLNRLWTSDAPISHAGKYYTLHNAVL
                     LTRPKEKVPVYVSAIGPRAADLAGRLGDHLITLANNPHYVREELLPALEAGARAAGKD
                     PKPMERVGHFAYVYDPEQVVTPEHLQQDAGTIQGGAMAKAFSSEFISIFHTPEDFIRK
                     IEAMERMGYTHLAIADQRMGIVKGILPSLDEAAGLKVWKDVLPHVR"
     gene            2756..3724
                     /locus_tag="PK154_02800"
     CDS             2756..3724
                     /locus_tag="PK154_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPW10022.1"
                     /translation="MKTKIGYFAITDAWTPAECLQQAVAAEKTGFDSLWVEDHLIAMP
                     GGTECNFAWTVMSSALQATKKVPFITGVTAPIMRYHPAIVAQAFATMGAMYPGRAGIG
                     VGTGEPPNEMAVYPGEWPSNNARLEMLEEALSVMNRLWTSDRPINQTGKFYTLKDAVL
                     LTRPKEKVPVYVSAIGPRAADLAGRLGDHLITLANNPQYVREELLPAFEAGARAAGKD
                     PGTMERVGHFSYLYDPDQVIKPEQIQQDAGTIQGGAMAKAFASGVISIFSSAEDFIKK
                     IEEMKGMGYTHIAIGNVSTMAEKGSRVPSYEAPSLKIWKEVLPHVR"
     gene            4060..5202
                     /locus_tag="PK154_02805"
     CDS             4060..5202
                     /locus_tag="PK154_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10023.1"
                     /translation="MITMKNDKIFTNRFILILFLVIFLMFAAGVVSALPQPDTASQSA
                     FNSPVVRKTYSPESYSYAQKNFSYTPDTFPTRIKTPVIQPIKSPTIRPTSIPGEQPTK
                     TPPIRPNKTPVIQPIKTPLLPPTKTPPVQPTNSPPSAEVQQLVQNLKGEYQTSMAARE
                     RLVQIGPSTIDAILPLLRQQDYYVRFAAADTLRRLNYIPASPEELAYYIIGLREYDTG
                     LYIENFNKLVAANSAAVDPLILVLKDSNWMKGSDIDTIEVLVAIGKPAVPKLIQALND
                     PVSRESAGIALVLIDDAKGRQAVINLMNQQGVSLSYYAKNYKQIIKNYDPYDTTRLDL
                     LWLGLALDEYGTKEMAEYYVNSGSTLYDFGEKWAKAHGYRITFKGL"
     gene            complement(5594..5749)
                     /locus_tag="PK154_02810"
     CDS             complement(5594..5749)
                     /locus_tag="PK154_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10024.1"
                     /translation="MRTGLFDVSISASFVPIYVRSQPHRGQVTRMLVNISTHLFIVSL
                     DRPLAEC"
     gene            6026..6364
                     /locus_tag="PK154_02815"
     CDS             6026..6364
                     /locus_tag="PK154_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10025.1"
                     /translation="MVAEKRPVLLFTTYKFRSGTNLSVRRGIRWASRLDIGDTIMVQP
                     REGGEQQKALVTGLKVKRFRDIREQELSVEHDPQCHTWNGLFSTMKKLFDDFDETEIV
                     TMIFFTLEGE"
     assembly_gap    6374..6473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6756..7217
                     /locus_tag="PK154_02820"
     CDS             6756..7217
                     /locus_tag="PK154_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10026.1"
                     /translation="MSPRIRIIILIVASIILLIVLSHSGELLPVSDMPSELPDQFPTG
                     SGNASPLFSLTVTPMQAHAHPGDPVDCAVLITPYNGFDDPVELELKVDAGPVFKGTYH
                     AGTMTPPYPKTYEYRVNVPEQSPAPVTVNGTLMARGGGFSDKVDLVLFIES"
     gene            7435..9792
                     /locus_tag="PK154_02825"
     CDS             7435..9792
                     /locus_tag="PK154_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPW10027.1"
                     /translation="MISFTLQVFASLLAGILCGLFFGEAAQVLAPFGTIFIGLMQVTI
                     IPSVVIFIITGIGSIGHEDAKKFLLTVAAVLLLIWFLGVLIFFYMQFAFPDVQNPALF
                     STTQSAVTDEIDMIDLFIPSNPFSSLAEGLLPAIVLFCILLGFALIGDVKNRPFVDML
                     QSLSAAFSKITRWMMMIVPLGIFAITASTFGTITFTQFLEIQVYLITTIVISVLLVLL
                     VLPLLTYSLTPFGFREILSAANKGVLMGFSTSHVFITMPLLTEGVASLFKGLRNGKEV
                     PAAGEAIVPGEGAVTTEEETRTRKGEAATGGERSRTSGGEALGPDEGPCPSHREAVTI
                     EEKAKAYSGVLIPLTYTIPSLGVFIVLLFVLFTAWFYGDPFGIREQTFLALAGVPSLF
                     GSPKLSLQFILELMRLPADAMQLYLISNPFHVYFASALTCMSMFSLSAIVTAFLTGLG
                     GLRAKRALISLVIVILVLSGAVIGLRLGFTSLLGDSPEGGETVLNMKMPPEVSGGEID
                     TTIYQPYSEVSPRQDMFPAEESLLRRIKSRDLLRVGYNPDMMPLAYFNRDGDLVGYDI
                     HMAHDLARTLNVSRIEFIPVDRETFLDGVNNGGCDIVMSGVVLVPGISDEARFTSPYL
                     DLHLAFVTRDDRKDEFEQWDEVVKKKDLVIGIADTSAYYQTAIRLFPNATFIPLESPE
                     QFFNQTEAEALLTTAEVGTAMTLLHPFYDVAILQPSDIYQIPCVYVVSRDCDDASLMF
                     LNYWLEMEEKYGSLDKKYRYWVLGEDAEHQPPRWSVIRDVLHWIE"
     gene            9936..10208
                     /locus_tag="PK154_02830"
     CDS             9936..10208
                     /locus_tag="PK154_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10028.1"
                     /translation="MNTSIEGNIMSTSGLHETVGISLERSTEIIDLFYDHFLDGCRRD
                     PDPLVFTGDALQWILHEPTLSIEEMVYLTYFFTRIILPDAGEGADT"
     gene            complement(10315..10707)
                     /locus_tag="PK154_02835"
     CDS             complement(10315..10707)
                     /locus_tag="PK154_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPW10029.1"
                     /translation="MSFLGITLKNPIRGNRIKRAVGSRCEVCGCEEYLENLVVHSIIK
                     EEEGCDQSPGSMEPFLLVLCFRCHNAIHAFDAPRSSQEELARQRPEPLRREIRRILSS
                     VPRRYTPPDTDMEEAYIVACTSHFRFGV"
     gene            complement(10869..12182)
                     /locus_tag="PK154_02840"
     CDS             complement(10869..12182)
                     /locus_tag="PK154_02840"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW10030.1"
                     /translation="MSPQDPIVPLIEQMVVDAYPSTAPGAALLLVRQGEVLYRRGIGL
                     ANLEHQVPMTPDMPFCLASLTKPITATAVLMLVEAGRLALTDSLTDLLPDYPSGMAPI
                     TVEHLLTHTSGIPEFSEIPEWWAVHRQEVTVDQLIDLFKHHPHSFAPGTRWSYCNSGY
                     ILLGAIIEKVSGKSYRDFLDEHMFSPLGMMHTCYEPIPGRVIPGMVSGYTKTPDAYLH
                     AEYFSTSHFFAAGGLVSTADDLARFCTALCTGNLLNAETLRRMWTPARLPDGASTHYG
                     CGWWVSTCQERPVMEHYGIRPGYANQLLVLPDDNILVIVLSNDDGNLNQTEHLAVEMA
                     ALALRNPYQPPARFPFSSDELSHFAGDYTTSEGKHLVVVSEAGKLVMQGTPEERFALQ
                     PLSPLEFFFPEIPESRLVFSHKKDRITGFLWSPRRGMPLHARKTE"
     gene            12522..13376
                     /gene="nudC"
                     /locus_tag="PK154_02845"
     CDS             12522..13376
                     /gene="nudC"
                     /locus_tag="PK154_02845"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPW10031.1"
                     /translation="MIPEERTVPFATRGLVYHRNLPPDTREQWILVDGPSVVTGKDGT
                     MLTNPAACRIFLADDPDRFMIGYDHRGSWYAVPISSSVLLPDGYARTHLRSLYGVIPD
                     DLLGIASRAVALSRFSDTHRFCGICGAETNRKEDEIATVCPACGHIGYPVINPVVIVC
                     IRRDDTILLARSPRFLPKVYGVIAGFVEAGESLEQAVAREVAEEVGIGIDTIRYRCSQ
                     PWPFPHSLMVGFTASYAGGTIRINPAEIEEAGWYSYDSLPTLPAAGSITRILIDQVLE
                     EISRDTKC"
     gene            13510..>14185
                     /locus_tag="PK154_02850"
     CDS             13510..>14185
                     /locus_tag="PK154_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10032.1"
                     /translation="MILEAAGWCVLATSSLIIGAWIAFRFQPSSKVVGLVMGFGAGAL
                     IAAVAYELFPEPSYQHLLSFISLGLGAVLFYLGTSLIERRSGGKGTGDANEQTRMGKT
                     IVLGALLDGIPESLVLGMTLAVGGALSIAFFGAVLVSNLPEGIAASAELERGGIPRHK
                     IYSLWIFVMLISVISTIVGVMVIQIVPSAEGVNAMAIAGGAVLAMLADSMIPEGFREG
                     GRLTGLI"
BASE COUNT         3177 a         3881 c         3844 g         3183 t
ORIGIN      
        1 cgccacgcgc tcatacacgc ccctgtcagc cgtgaagatc acgatcgtct gtttgcggaa
       61 gggggtgccg ttcccctggg tcttgatcag ggcgctgttg agcggatcgt ttacggcggc
      121 gaacatccag tcccctttgg gaatgaggct ctccttgtgc cggacccaga gaaagaccgt
      181 gaagctgaag tagtcgcacg gaggcggtgt cggccccagg tagacaacag cctcgtcagg
      241 catgagccga aaaatcgtca accctccctc aaaaggattt tcctcgatga aaggggagat
      301 cggaaccatc agcactttgt aggtttggcc tgcgttgttg ccgttggctg aatcaccgag
      361 gccggagtcc agcagatcga cgatcggatt gacgggggtc atctggccgt tggtgacaat
      421 aaacccattt tcttccaggg ttgcccgaaa agacgaaacc ctgccgtggt cctcgtcgct
      481 ggcgcccggt gagtccggca cggtgggctt caacggctgg gtggaagcag acggtgaatt
      541 gatggttgat ttcactggtg cggatgctgc agggataaca agtgctccaa caagacacag
      601 gataaaaacg aatgctactg ttttattcat ggttcatgaa atattttcaa gaatcataaa
      661 tgctccgtag ttttcatggc gaagtactct ctcaggacaa tccagcgctc tgggtgatcg
      721 aagaatggtc atcctcgatt ccaccttcac tctgcattcc ctcccttttt atagcaacat
      781 cgcctaccgc tgataaggag ccagtcgcat gctaccccgg gttatccttc atgttgccac
      841 cagtctcgac ggtcggatca cgaacttccc ggcgaatctc gacctgtatt atgcccttgc
      901 ggcgacatgg aagcctgatg ctatcctgtt tggaagcgag acagttgttg ccgctgtccg
      961 cgagaacccg tcgctcgaag tgccaaagga acacgaggat ctgttcaccc cgccggaggg
     1021 tgcagaagac acccggccgc tcctggtgat cgccgacagc cgggggagag tccggtgctg
     1081 ggatgccatc cggacctggc cgtacatgcg ggacctcctt gcgttctgtt ctgctgccac
     1141 cccgaaggag tacctcgatt acctggcaga gcggcggatt gacgcaatca tcgccgggga
     1201 ggaccggatc gacatgcgtg aggcattgtc agagctgaac aggcggcatg gcgtgaaaac
     1261 ggtccgggtc gattccgggg ggaccctcaa cagcgtcctc ctcgaggcag gggtggtcgc
     1321 ggaagtgagc gtactggtcc accccttcct cgccgggggg aagccggacc cgaccatgtt
     1381 cgacccggcg aaggcaggct tcccggatct ccaggtcccc ctggagctca tccggaccga
     1441 agtcgtggga gacggcctgg tctgggcgcg atattccgtg aaacagtcgc cgtgaaatcc
     1501 tctcaatcgt acccgtgatc accggaagat ccggtccgtt gttcttatga ttatcatgag
     1561 atctgctcca tcggagaagt aagatacctc actcaccctt ctcttctcca cacctgttcg
     1621 tcagatgcat gaggaaaaga aggaatgaat ttattcaccc ggggcgtaac catcacatgg
     1681 tcacatgacc cctgtccgct ctacggatcc aggaggagga atgagtatgg gaataaagat
     1741 tgggtatttt tcggtaaccg atgccatgac cccggccggg tgtttacggc agtcaataga
     1801 ggctgagaag atcggatttg actcgttgtg ggttgaagat cacctgattg caatgccggg
     1861 agggaaggaa tgcagcttcg cctggacggt gatgagctcg gcgctccagg ctacgaagaa
     1921 ggtccccttc atcaccggcg tcaccgcccc tatcttgcgg taccatccgg cgatcgttgc
     1981 ccaggccttc gctaccatgg cggcgatgta cccgggacgc gtgggactcg gcgtgggcac
     2041 gggcgagccg ccgaacgaga tggccgtcta cggcggcgaa tggccctcga acaacgcccg
     2101 gctcgagatg ctcgaggagg ccctcaccgt cttgaaccgg ctctggacga gcgatgctcc
     2161 tatcagccat gccgggaagt attacaccct tcataacgcg gtcctgctga cccggccaaa
     2221 ggagaaggtc cctgtgtatg tcagcgctat cggaccccgt gcagcagatc tcgcgggacg
     2281 cctcggcgac cacctgatca cccttgcaaa caatccccac tacgtcaggg aagagctcct
     2341 gcccgctctc gaggccggcg ccagggcggc agggaaggat ccaaagccca tggaacgcgt
     2401 gggacatttt gcctacgtct acgacccgga gcaggtggtc acccctgaac acctccagca
     2461 ggatgccggc accatccagg gaggcgccat ggcaaaggca ttttcgagcg agttcatctc
     2521 gatcttccat actccggaag atttcatcag gaagatcgag gcgatggagc ggatgggata
     2581 cacccatctt gcgattgccg accagagaat ggggatcgtg aaagggatcc tcccctccct
     2641 ggatgaggct gcgggcctga aggtctggaa ggatgtcctc ccgcacgtca gataatcacg
     2701 gtctggtacg ggatgaaagg cagataaaga acagaactgg gagaaagaaa gaaccatgaa
     2761 aacaaaaatc gggtattttg caatcaccga tgcctggaca ccggccgagt gcttgcagca
     2821 ggcagtagca gctgaaaaga cagggtttga ctcgctctgg gtcgaagatc acctgattgc
     2881 catgccaggg gggaccgaat gcaatttcgc atggacagtg atgagctcag cccttcaggc
     2941 gacaaaaaag gttcccttca tcaccggcgt caccgcccct atcatgcggt accatccggc
     3001 gatcgttgca caggcgtttg ccaccatggg agcgatgtat ccaggacgtg caggcatcgg
     3061 tgtggggaca ggcgagccgc cgaacgagat ggccgtctac cctggcgaat ggccctcgaa
     3121 caacgcccgg ctcgagatgc ttgaagaggc tctcagcgtc atgaaccggc tctggacgag
     3181 cgataggccc atcaaccaga ccgggaagtt ctacaccctg aaggatgcgg tcctgctgac
     3241 ccggccgaag gagaaggttc ccgtctacgt cagtgcgatt ggaccccgtg cggcagatct
     3301 tgctggacgc ctcggtgacc acctgatcac ccttgcgaac aatccccagt acgtgaggga
     3361 ggagctcctg ccggccttcg aggcaggtgc cagggcggcg ggaaaggatc caggcaccat
     3421 ggaacgcgtc ggccatttct cttacctcta cgacccggac caggtgatca agcctgagca
     3481 gatacagcag gatgccggca ccatccaggg aggtgccatg gcaaaggctt tcgcaagcgg
     3541 ggtcatctcg atcttttctt cggcagaaga tttcatcaaa aagattgagg agatgaaggg
     3601 gatgggatac acgcacatcg cgatcggcaa cgtgagcacc atggctgaaa aaggcagccg
     3661 tgtgccttct tacgaggctc ccagtctgaa gatctggaaa gaggtccttc cgcacgtcag
     3721 gtaacagcgg actggtgttc aggaaagatc gggataatcc ctctttttta tatgagaatg
     3781 cctccggcag tttcatcttt tatcattgtg gccctcaaag gaatcatgtt atttttttta
     3841 aggaataacg ttctgtcaac gtggtatttc atcaggtgca gcaatcccac catgactcga
     3901 tctcaatcat gatctggtgg tgtttccccc ctctcctgat tgacagaagg gtgaacatga
     3961 tcgttctcct cgtacaatca acacatcatc gcaggcagcc gacgcggcga atatcaagtc
     4021 atgaggtgta agtatataac taccaaaaga aattattgga tgataaccat gaaaaatgat
     4081 aagattttca ctaataggtt tatccttata ctttttttgg tgattttcct catgttcgca
     4141 gcgggtgtag tatcagctct ccctcaacca gatactgcca gtcagagtgc ctttaatagc
     4201 cctgttgtca ggaaaacata ttccccagaa tcgtatagct atgcccaaaa aaattttagt
     4261 tacacacctg acacttttcc tacacgaata aaaactcctg ttatacagcc tattaaatct
     4321 cctacaatac gccccacttc cattcctggt gaacaaccta ctaaaactcc tccaattcgc
     4381 cctaataaaa ctcctgttat acagcctatt aaaacacctt tactaccccc tacgaaaact
     4441 cctcctgtac agcccaccaa ttcaccaccc tctgccgagg tccagcagct cgtccaaaac
     4501 ctgaagggag aataccagac ttcgatggcc gctcgggaga ggttagtcca aattggtcca
     4561 tccacgattg acgctatcct gccgctcctg cgccaacagg attattatgt tcggtttgct
     4621 gcagctgaca ctctcaggag actaaactac attcctgcat ctcccgaaga gctggcttac
     4681 tacataatcg gattgaggga gtatgacacg ggtctttaca tcgagaactt taataagctg
     4741 gtggcagcca attcggcagc agttgatccc ctcatccttg ttctcaagga ttcgaactgg
     4801 atgaaaggga gtgacataga tacaattgaa gtcctggtgg ctatcggcaa acctgcggta
     4861 ccaaaactta tccaagcatt gaatgatcca gtgagccgag aatctgcggg aattgccctc
     4921 gttcttattg atgatgcaaa ggggaggcag gcggtgataa atcttatgaa ccagcaggga
     4981 gtcagcctct cctattatgc aaaaaactac aaacagatca taaaaaacta tgatccctat
     5041 gatacaactc gtctcgacct cctttggctt ggtcttgccc ttgacgaata tggtacaaag
     5101 gaaatggcag aatattatgt caattcaggt tctactctct atgatttcgg agagaagtgg
     5161 gcgaaagctc atggttatcg catcacgttc aaggggctat aaatctgcac tattattttt
     5221 aacttattct cccgacaatt cctcccagga gattgtatcg atatgtatta ttgacccagc
     5281 tgattttctc tcttcactca cctgagctca tgacaaatcc tgattcattt ttctgtcctc
     5341 gctctgaccg gatgattgtc caacgaatca tccgggccct tcatgggctc tcggggcatg
     5401 aatcatcgtc ccttcgatca tttcataaga tccgattgaa gagggaaact ggtatatcgc
     5461 aatttttagg agaaagggtc tctgtatttc ggttgtacag ggtcaaggcc tggtactctc
     5521 cctgccatcc actttcaccc ctcataccgc ctcaggattt atgctgcaaa gaagatggga
     5581 acatgatccc tcttcagcat tcagcgaggg gtcgatccaa agagacaata aagaggtggg
     5641 tcgagatgtt gacaagcatt cgggtaacct gtccacggtg cggctgggaa cggacatata
     5701 tgggcacaaa acttgcgctg atcgagacgt caaaaagacc cgttcgcatc aggtgttcaa
     5761 agtgtgcaac gagtatcagg atcaatccca gaacgccatg aacgagggga ataggcgagg
     5821 gaatccttcc cgggtgagcc ttccggggca gcagtccgag aaccctctca gggatgaaac
     5881 gctggaatct tcctctccgc tatctgttcc agcatgaccg ggttcctcct gttttcccaa
     5941 ccattgatgg caccggactg atcgcataac actatcatta ttatctgcgc tacaaaagaa
     6001 gcatcatagc acaggaggga aacccatggt ggcggaaaag agaccggttc tcctttttac
     6061 aacctataaa ttccggagcg ggacgaatct gagtgtgcga cgagggatcc ggtgggcatc
     6121 ccgactggat atcggggata ccatcatggt acagccacgg gagggaggag aacagcaaaa
     6181 agccctggta accggattga aggtgaagcg gttccgggat atccgggagc aggaactctc
     6241 cgtggagcac gatccccagt gccatacctg gaacggcctc ttttccacca tgaagaagct
     6301 gtttgacgac ttcgacgaga cggagatcgt caccatgatt ttctttacct tggaaggaga
     6361 gtaactcctc cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacggag
     6481 atcgtcacca tgattttctt taccttggaa ggagagtaac tcctcctttt ccgatgacac
     6541 tgcctagaga ccaaccacca cgcaaaaaac cagtaggctc gcagaggtcg ttgagagaac
     6601 tgacctgctg atcggggact ctgctcctgt gacctgatga actgagaaac cttgatgatt
     6661 caagatacat cacactcggg atgatcgaat atgttccccg tcccttttac agggaagaaa
     6721 agttcaaagc ataacaatgg aaagtactga aaacgatgag tcccaggatt cggatcatca
     6781 tcctcattgt cgcttccatc attcttctca ttgtactctc ccattcgggg gagctccttc
     6841 ccgtctccga tatgccatca gagctcccgg accagtttcc aacaggatca gggaatgcct
     6901 cccccctttt ttcccttacc gtgaccccga tgcaggccca cgcccatccc ggtgatccgg
     6961 ttgattgtgc agtgctcatc accccctaca acgggttcga tgatccggtc gaactggaac
     7021 tgaaagtgga tgcaggaccg gtgtttaagg gcacctatca tgcagggacg atgactcccc
     7081 cttatccgaa gacatacgaa tatcgggtga atgttccgga gcaatcgcct gctccggtca
     7141 ccgtgaacgg aaccctcatg gcaagaggtg ggggattttc ggacaaagtc gacctggttc
     7201 tgttcataga atcatgaacg agtgccgatg ccggtgcatg atccaaccga ttacagagac
     7261 ccacccctct tcacgcgatg gatttcacag ttctctcatc gataaatcca gacatttatc
     7321 tcctggagga gcctaatcca gcatgtatcc ccaacgtttc gagatccacg caaaagaata
     7381 ccaatcgtcg ctctctcgtg gattctgccc ggaggcagcc ggatgaaagc acccatgatc
     7441 agctttacac tacaggtctt tgcaagcctc ctcgccggaa tcctctgcgg gttattcttc
     7501 ggggaggctg cccaggttct ggcccccttc ggcactatct tcatcgggct gatgcaggtg
     7561 acgatcatcc cctcggtcgt catctttatc atcacgggta tcggcagcat cgggcatgaa
     7621 gatgctaaaa aattcctcct gacggtggca gccgtcctcc tgctgatctg gtttttaggc
     7681 gtcctgatct tcttttacat gcagttcgca ttccctgatg tccagaaccc tgctctcttc
     7741 tcaaccaccc aatccgccgt aaccgatgag atcgatatga tcgacctctt cattccgtca
     7801 aatccgttct cctcccttgc cgaagggctc ctgccggcca ttgtgctctt ctgcattctg
     7861 ctcgggttcg ccctgatcgg cgatgtaaaa aaccggccgt tcgtcgacat gctccagagc
     7921 ctcagcgcgg ccttctcaaa aataacccgg tggatgatga tgatcgtccc cctgggcatt
     7981 tttgccatca cggcaagcac cttcgggacc atcaccttca cgcagttcct cgagatccag
     8041 gtctacctca tcaccactat cgtgatctcc gtgctgctgg tcctcctggt cctgcccctg
     8101 ctcacctaca gcctgacacc gttcgggttc agggagatcc tctcggctgc gaacaagggg
     8161 gtcctgatgg ggttctccac ctcccatgtc ttcatcacca tgcccttgct gaccgagggt
     8221 gtcgccagcc tgttcaaggg actacggaat ggaaaggagg tgcccgcagc cggggaagcg
     8281 atcgtgccag gggaaggggc tgtgacaacg gaagaggaga ccaggacgcg aaaaggagag
     8341 gcggcgacag ggggggagcg gtcaaggaca tcgggaggag aggctctggg acctgacgag
     8401 gggccctgcc cttcacacag ggaagcagtc accatcgaag agaaagccaa ggcgtacagc
     8461 ggtgtcctga tccccctgac atacaccatc ccctccctgg gggtcttcat cgtcctgctc
     8521 ttcgtattgt ttacggcctg gttctacggc gatccctttg ggatccggga gcagaccttc
     8581 ctggcgctgg caggggtgcc gagcctcttt gggtcgccca agctctcgct ccagttcatc
     8641 cttgagctga tgaggctgcc tgccgacgcc atgcagctct acctgatctc caacccgttt
     8701 cacgtctact ttgcttcagc ccttacctgc atgtccatgt tctccctctc tgccatcgtc
     8761 acggcgttcc tcaccggcct cggtggactc agggcaaaaa gggctctgat ctctcttgtt
     8821 atcgtaatcc tcgtcctctc cggcgccgtc atcgggctcc ggcttggctt cacctcgctc
     8881 ctgggtgatt ccccggaggg aggcgagacc gtgctgaaca tgaagatgcc cccggaggtc
     8941 agcggggggg agatcgatac aacaatctac caaccctatt cggaggtgtc ccctcgccag
     9001 gacatgttcc ctgctgaaga gagtcttttg agacggatca agagccggga tctcctccgg
     9061 gtcgggtata atccggacat gatgccgctt gcctacttca acagggacgg tgatctggtc
     9121 gggtatgata tccacatggc ccatgacctg gcgagaaccc tgaatgtctc ccgcatcgag
     9181 ttcattcccg tagaccgcga gacgtttctc gacggggtca acaacggggg ctgcgatatc
     9241 gtgatgtccg gggtggttct tgtcccggga atatcggatg aggcgaggtt cacctcaccg
     9301 tatctcgatc tgcacctggc cttcgtcaca cgggatgacc ggaaggacga gtttgagcag
     9361 tgggacgagg ttgtgaagaa aaaagacctg gtgataggga ttgccgacac gtcagcttat
     9421 taccagaccg cgatcaggct ctttcccaac gctacgttca tccccctcga atccccggaa
     9481 caattcttca accagaccga ggccgaggct ctcctgacca ctgccgaagt ggggacagcc
     9541 atgacactgc tccacccctt ctacgatgtc gcgatcctgc agccgtctga tatctaccag
     9601 atcccttgcg tctatgtggt ctccagagac tgcgacgatg cctcactcat gtttctcaat
     9661 tactggctcg agatggaaga aaaatatggg tcgcttgaca aaaaataccg gtactgggta
     9721 ctcggagaag acgcggagca tcagccgcca cggtggtctg tgatacggga tgtcctgcac
     9781 tggatcgaat gacggtatcc cggttttcgc cgctgacttc cgctcgatca cccgagctat
     9841 tctccaggct gggtggagga aaacgacatc tcacccgatg agtgatggtt gcaggggacc
     9901 tgattcccct gggctgaaat atttatggtg cacgaatgaa cacctccata gaagggaaca
     9961 tcatgagcac gtcaggtctt cacgaaactg tcgggatatc cctggaacgg agtaccgaga
    10021 tcattgatct gttctatgac cattttctgg atggatgcag gagagatcca gaccctctcg
    10081 ttttcaccgg tgatgccctc caatggatct tacacgaacc gacgctttct atcgaagaga
    10141 tggtgtatct tacctatttt tttaccagga tcatcctccc ggatgcaggc gaaggtgctg
    10201 atacataagc atcaggccac cgaccctcct ctcctctcct gttcaattac ggagatcagg
    10261 tattgagaac ggactggccg gggagtgaat acccttcccc ccttctctcg ctgatcagac
    10321 cccgaacctg aaatgactgg tacacgcgac gatgtacgcc tcctccatgt cggtgtctgg
    10381 cggagtgtat cggcggggga ctgaggagag tatccttcgg atctcccggc ggagcggttc
    10441 gggccgttgc ctggccagtt cttcctgtga gctgcggggg gcatcaaaag catggattgc
    10501 attatgacac cggaagcaga ggacgagcag gaacggttcc atggaccccg gtgactgatc
    10561 gcacccctcc tcctctttga tgatggaatg caccacgagg ttctcgagat actcctcaca
    10621 gccgcacacc tcgcaccgtg aaccaaccgc ccgcttgatc ctgttccctc tgatcgggtt
    10681 ttttagagtg atgccaagga aggacatagg acgggtatcc tcacttccgg aagagtggtg
    10741 caggggcata aaggttgtac tgcgggattg tgttcaggat cctggcttgc ccagtcgttc
    10801 gtgatcattt tgggtcgttc tgatgctcga acctgaaacc atgggaaaga aaaaaattcc
    10861 gcctcccctc attcggtttt cctggcgtgg aggggcatac cccggcgagg cgaccagagg
    10921 aatccggtga tgcgatcctt cttgtgcgaa aaaacaaggc gtgattccgg gatctccggg
    10981 aagaaaaatt cgagcgggga gagcggctgg agcgcgaagc gttcttccgg ggtgccctgc
    11041 ataacgagct tcccggcttc ggataccacc acgaggtgct tcccctcact cgtggtatag
    11101 tccccggcaa agtggctcaa ctcatcggag gagaagggga agcgtgcagg tggttgatag
    11161 gggttgcgga gcgccagtgc agccatctcc acggccaggt gttccgtctg gttgaggttc
    11221 ccatcatcat tcgataagac gatgaccaga atgttgtcat ccggcagtac gagcagctgg
    11281 tttgcatatc ccggcctgat gccataatgc tccatcacgg gtcgctcctg gcaggtgctt
    11341 acccaccagc cacagccata gtgggttgag gcaccatcag gcaaacgggc gggggtccac
    11401 atcctgcgga gggtctctgc gttgagcagg ttcccggtgc acagggcagt acaaaagcgt
    11461 gcgaggtcgt ctgcggtcga gaccagccct ccggcggcaa agaagtggga ggtgctgaaa
    11521 tattcggcat gcagataggc atcaggcgtt ttcgtatacc cgctgaccat gccagggatg
    11581 acacggcccg ggatcggttc gtagcaggta tgcatcattc cgagcggcga gaacatatgc
    11641 tcgtccagga agtcccggta gctcttcccg gagacctttt cgatgattgc tcccaggaga
    11701 atatagcccg agttgcaata cgaccaccgt gttcccgggg caaaggagtg tgggtggtgt
    11761 ttgaagagat caatcaactg gtcaacggtc acctcctggc gatgcaccgc ccaccattcc
    11821 gggatctcgg agaactcagg aatccctgag gtgtgcgtca gcaggtgttc taccgtgatg
    11881 ggtgccatgc ccgagggata atcgggcaga agatcggtga gcgaatcggt aagtgccagc
    11941 cggccggctt cgaccagcat cagaactgct gtcgcggtga taggttttgt cagggatgcc
    12001 aggcagaacg gcatgtcggg cgtcatcggg acctggtgtt cgaggttggc gaggccgatt
    12061 cctctgcgat acaggacctc tccctgtcgg accaggagca gggctgcacc aggggctgtg
    12121 gatggatacg catcgacgac catctgctca atgagtggta cgatgggatc ttggggggac
    12181 atcgtgggct cctctcttta agtgctgaat agtgtcagag atgatgtaag tatcttcaga
    12241 taccgtgagg agccatggct actggcattt ccaggaaggt tattcattgg cagattccag
    12301 aagtgaaccg aagattacgt tgaatacaaa ccatcgggat cccccatttt gccggttcca
    12361 ccgtaccgat ccttgaatac gttatgagag ataccagtga tagcaaacca ccctgaaacg
    12421 ggagcagagc aggttccgca ggacatgact cccttttcca ggaagtgccc caatagagga
    12481 acgaaattga agataccact atccctcaca ggagagcata cgtgattccg gaagaacgta
    12541 ccgtcccctt tgccacccgt ggcctggtct atcaccggaa tcttcctccc gataccaggg
    12601 aacaatggat ccttgttgac ggaccgtcag tcgtcaccgg caaagacggg acgatgctca
    12661 ccaatccggc cgcttgccga atttttcttg cggatgatcc cgatcggttc atgatcggct
    12721 atgaccaccg tgggagctgg tatgcggtcc cgatcagtag ttcagtactc ctccccgatg
    12781 gatatgctcg gacccacctc cgttcccttt acggggttat ccccgatgac ctgctcggca
    12841 ttgcgtcacg tgccgttgct ctctcccggt tctccgatac ccaccggttc tgcgggatct
    12901 gtggggcgga gacgaaccgg aaagaggacg agattgccac cgtctgcccg gcctgcggtc
    12961 atatcggtta tccggtcatc aaccccgtgg tcatcgtctg tattcggcgg gatgacacga
    13021 tcctgctggc acgatccccc cgctttctcc caaaagtcta tggggtcatc gccggatttg
    13081 tggaagcagg ggagtccctg gaacaggcag tagcccgcga ggtcgccgaa gaggtaggaa
    13141 tcgggattga taccatcagg taccggtgca gtcagccctg gccgtttccc cattccctga
    13201 tggttggctt caccgcctcg tatgcagggg ggaccatccg gatcaatccg gccgagatcg
    13261 aggaagccgg ctggtactcg tatgattccc tccccaccct tccggccgcc ggaagcatta
    13321 cccggatcct cattgaccag gtgctagagg agatatcccg ggatacgaaa tgctgacgac
    13381 catatggcat ggaatgaaaa acactgatgc ggagatctgt tgtacatcgc catgctatat
    13441 acctcaacgc agagatctat ttcaatcggt aaaggaaccc gaacagacat ggaatcccgg
    13501 tgatggcaga tgatccttga agcggctggc tggtgcgttc ttgcaacctc ttctttgatc
    13561 attggagcat ggatcgcgtt tcggttccag ccatcctcaa aggtcgtcgg cctcgtcatg
    13621 ggcttcgggg caggggcact gatagccgca gtggcctacg agttattccc tgagccctca
    13681 tatcagcatc tcctctcgtt catttccctt ggtctgggtg ccgtactatt ctatctcggc
    13741 acctcattga tcgaaagacg ttcaggggga aaaggaacgg gggatgcgaa tgaacagacc
    13801 aggatgggaa agaccattgt tctcggggct cttcttgacg gtatcccaga gtcccttgtg
    13861 cttggaatga ccttagccgt tgggggtgcc ctgagtatag cgttctttgg tgcggttctg
    13921 gtctcgaatc tgcctgaggg catagccgct tcagcagagc tggaacgggg agggattcca
    13981 cgccacaaaa tctactcgct gtggattttc gtgatgctca tcagtgttat cagcactatt
    14041 gtcggtgtca tggtgattca gatcgttcct tccgccgaag gggtgaatgc tatggccatt
    14101 gccggtggag cagttcttgc gatgctggcg gattcaatga taccagaagg gttccgggaa
    14161 ggggggaggc tcaccgggtt gattg
//
LOCUS       DAOOCO010000028        13889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4030_length_13889_cov_4.840538, whole genome shotgun sequence.
ACCESSION   DAOOCO010000028 DAOOCO010000000
VERSION     DAOOCO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_13889_cov_4.840538"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PK154_02855"
     CDS             <1..1730
                     /locus_tag="PK154_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPW10033.1"
                     /translation="SRGDRIIVTELPYQVNKAQWITAIADMVKEKRIDGISDIRDESD
                     KEGIRVVFELRKGAIAPVILNNLYKHTALESSFWAINYAIVDNQPKILTLTGLLEEFV
                     RHRVQVIRARSEFDLKKAQERVHILDGLLLALARIDEVIATIRASDTADTARAALIST
                     FGLSEPQATAILQMQLRRLAALEQQKILDEKHGLEGEIARLQDILASEANIRAEIRRE
                     TDEIAAKFGDARRTQIALDMSELSTEDLIEDKTVLVSITTANYIKRTDIDSYRKQRRG
                     GHGVTGMTIKEDDVVDSVFVAGMKDYLLCFTNTGRVYWLKVYQIPESSRVAKGKPIVN
                     LLNLKDEVVTTVIPIREFAEDQHLLFATRLGQVIKIPLDQFSNPRSVGTNAIRLRGGD
                     QLVDVIMTDGTKEIILTSRFGQSLRFHEETIRTVGRNAQGVRGMKLRGGDTVVALTLL
                     EKDHLLTLSDVGFGKRTEFDEFRGHGRGTMGVRNMQLDRDAVVIASCAVSQSDEILVM
                     TASGIVIRTPVSEIRVIGRGTKGVRIMRLGENDKVVGIAVVPADVDGNDQNGADSEKE
                     PTPPASPEP"
     gene            2042..2455
                     /locus_tag="PK154_02860"
     CDS             2042..2455
                     /locus_tag="PK154_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPW10034.1"
                     /translation="MYRKEIQVTTSHEGDIVDITPQVREIVRGSGIPEGVAHVFVAGS
                     TAALTTIEYESGVLADLSRALSTIAPDSIPYAHDRRWGDGNGRSHVRASIVGPSLSLP
                     VADHDLLLGTWQQVVLLELDVRKGRSRTMYVSVID"
     gene            complement(2610..2873)
                     /locus_tag="PK154_02865"
     CDS             complement(2610..2873)
                     /locus_tag="PK154_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HPW10035.1"
                     /translation="MPNPEKTIDNCILMLQHIMDDSTIPRNIRRVADETRQLLLNQSK
                     GTGLRAAEAISKIDEISNDPNMPVHARTRIWELVSQLETIPLD"
     gene            3078..3452
                     /locus_tag="PK154_02870"
     CDS             3078..3452
                     /locus_tag="PK154_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoantigen p27 domain-containing protein"
                     /protein_id="HPW10036.1"
                     /translation="MPTPVKREDDIMAGYLLKGGKMLSKSCPACGCPLFEIKGETLCV
                     VCREDEVSQHPPPPAAAPLPVPADQEVPAGTAPSFPSLSAALEETLIELCRRIRDERD
                     PAQVLILANAVRSGIKALKQLR"
     gene            complement(3491..5740)
                     /locus_tag="PK154_02875"
     CDS             complement(3491..5740)
                     /locus_tag="PK154_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW10037.1"
                     /translation="MSWITHPYIRTGTLESRTYQLSIAMRALDGHTMVVLPTGLGKTA
                     IALIVAASRLYNEKGKVLMLAPTKPLVEQHYRFFRRFIVIDGNDPECALFTGETPPDQ
                     RREEFSRARLVFATPQVIKNDLLSGAYTLSDVTLLVVDECHRAVGNYAYVFIASVYRE
                     TARSPLLLAMTASPGGQEMKVEEVCRNLGVTIVETRTEQDSDVAPYIHEREIQVIEVD
                     LPLSLQSAVVDLNELLDARIALIRELGFDLPRRQQLTMRVLNALNGQIQERIQQRDAA
                     GYTAASLYAELMKLRHAVSLAESQGSVVLAGYIHRLFSEGSSAGGSKAAARLVKDPVF
                     IRLLERTMSWETELHPKLEILASVTQDQLTRYPGSRIIIFATFRDMVQQIVEYLGKKG
                     IRAERFVGQAARDADRGLSQKKQIDCLRRFREGEFPVLVATSVGEEGLDVPSTDLVIF
                     YEAVPSEIRSIQRKGRTGRSGSGRIVVLVTKGTSDETYRWVSQSRERAMISGIRTLGS
                     RMEARAGEDQTEQVGERPIQKRIGEFMPQGPAIVVDDRETSSKVVEHLHSLGASLSVQ
                     RLPYGDYLAGDRIVVERKTTRDFVDTLIERDLFGQLKGLAGHALRPVLILEGGDLYSE
                     RDVNPNALRGALAAIAIDLGITPFFTRDPEETAQMLIVLARREEEGGERTGRLPFRKA
                     YQSVTEEQERIIGSFPEIGLKNARLLLDHFRTIQAIANASTEELAMVKGIGEKRAKRI
                     ADLVRKEYR"
     gene            complement(5746..7461)
                     /gene="glyS"
                     /locus_tag="PK154_02880"
     CDS             complement(5746..7461)
                     /gene="glyS"
                     /locus_tag="PK154_02880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPW10038.1"
                     /translation="MSDIYEKIIDLAKRRGFIWPTSECYGSVAGFIDYGPLGSMMKRN
                     IENIWRKFYIIGEGYYEIECPVIGQEEIYVASGHVKGFSDKMCQCPHCNEYLRADHVA
                     EGRGIAGASAMKSEALASAIATLPCPACEEVLGPVEVFSFNLMFHTTIGPGSQRTGYL
                     RPETAQGIFTDFSRLLRFYRDKLPFGTVQIGKSFRNEISPRQGMIRLREFTQAEAEIF
                     VHPGQKNHPRFQRYAGYEMPLFSAKQQMAGADPLVMTMREAVDGGVVANEYVAYYLAL
                     THDLLVRIGVSPDKLRFRQHLPDELAHYAEDCWDAEVFSGRFGWVETVGIADRTDYDL
                     RAHETQSGQRFSVFLPYEVPRRMNRVRVIPDMGALGPRFRGSAKAVADALTSCMPGPD
                     GITLTIDGKEVLITPDLYEVREEVVEVPGEEVTPHVIEPSYGIDRMIYGVLEHSYHEE
                     IVDEEERRVLRIPPAVAPIQVAVFPLMNRNGLPEIALEITDALKTRGILAEFDDSGAI
                     GRRYRRQDEVGTPFAITVDYDTKEDRTVTIRDRDSMRQVRVDAGRIPDLVRSLIDQTV
                     SFSEL"
     gene            7733..8341
                     /locus_tag="PK154_02885"
     CDS             7733..8341
                     /locus_tag="PK154_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HPW10039.1"
                     /translation="MQDDTRKTQLLQLFTNISGKTKIVEPMKKIHGTLRDREAVEREI
                     ALVMREILEQGLFKTKLRPIQLAKLVSAYYAGKNDTEIARELGDEKLSKTVARARVRL
                     KLFRDLDFKMPFDREKMESLLYSGKTMKEISDHLGVSPSTLREYRHVIEQRDDVTIDI
                     YLERIKDVMEDRDLSEVMTKSATKDGLEDSIDITEAEMVDVS"
     gene            8432..8740
                     /locus_tag="PK154_02890"
     CDS             8432..8740
                     /locus_tag="PK154_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10040.1"
                     /translation="MTLISCHDTLAISPRQDFCASRPLETSLLRQGLDSLSYSLLDHH
                     SDLLIHRIGTRKRAIEVSPRQGIKGIPFPGSIRWTTQDWQNPSSPQAPLPGISWVGNQ
                     "
     gene            8873..9526
                     /locus_tag="PK154_02895"
     CDS             8873..9526
                     /locus_tag="PK154_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPW10041.1"
                     /translation="MELTYYGHSCILLKGARTVLVDPFFPEGMTPPPADIVAVTHGHA
                     DHMGVAVDLKLPTVAINEVAKYLKSKGIPAEGMNIGGTITVEGVAFHMVQAVHSSWIE
                     EAGIGCNGGGAAGFVIGMEGKRVYHAGDTALFSDMKLIGELCHPDVALLPIGGRFTMG
                     PEEAMIAAQYIGAPLVIPIHYDTWPVIRQDPGLFRAALQRTTDILVNILAPGESLTI"
     gene            9680..9823
                     /locus_tag="PK154_02900"
     CDS             9680..9823
                     /locus_tag="PK154_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10042.1"
                     /translation="MLFHWDPVVVVRLPFRGETMFQQEIPLTREKGPVHRNEAPPDLI
                     TCY"
     gene            complement(10033..10356)
                     /locus_tag="PK154_02905"
     CDS             complement(10033..10356)
                     /locus_tag="PK154_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10043.1"
                     /translation="MLWKKRPGRSETKGSRKDPDLFSRVRNDVTVMRKQGEPPEKDAS
                     SFSRFHCDLCNSTYPSTGLKQCTLCGRWACSTCWTQDYYICNSCNGILKLHLLQGQEK
                     GRAKE"
     gene            complement(10325..10666)
                     /locus_tag="PK154_02910"
     CDS             complement(10325..10666)
                     /locus_tag="PK154_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10044.1"
                     /translation="MTPLEAVLIIIVVAILLFLIYYYWRGAKGEVSLSRPMESRVDEY
                     LDRRFELMIEEWSLVSRARLQSFRQSKDQVLLANEEKVASLKDFSETMESSLKTLEER
                     LDALEKKTWKK"
     gene            complement(10663..10887)
                     /locus_tag="PK154_02915"
     CDS             complement(10663..10887)
                     /locus_tag="PK154_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10045.1"
                     /translation="MVGMDSYMNAYLDRRMKNLIQDWDLATRNDIGDFENRLHSIEEA
                     TKEIESFETSAEITLENLEHRLLQLKGAMR"
     gene            complement(10887..11192)
                     /locus_tag="PK154_02920"
     CDS             complement(10887..11192)
                     /locus_tag="PK154_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10046.1"
                     /translation="MFEQAPFETAKKSSFRDSLAAIPGSIESRLPTMDKGLDRYFDAH
                     MSAIINEWGLITTHTLDDLDDRLDMVTTEIRNLERGRDEIEKRVAALEAGIRELEVG"
     gene            complement(11198..11608)
                     /locus_tag="PK154_02925"
     CDS             complement(11198..11608)
                     /locus_tag="PK154_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10047.1"
                     /translation="MAEELIVAAASPYWRWFESVFWILVLVLVLIIAYFIIKEIRLMT
                     NTRRLAEIDLEKEKLNLMKAEMAQRGTPFFRVSPEQMKEIQVLDDENAGLATDIFAKH
                     SMVDKRIERLENKVKLTKLDRMVEKIRDEERRIG"
     gene            complement(11627..12085)
                     /locus_tag="PK154_02930"
     CDS             complement(11627..12085)
                     /locus_tag="PK154_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10048.1"
                     /translation="MEGSVTLKVKVRTFPSKGRARVHESVLPMIGAKEGEALVLAQYP
                     TVWDKKTKTVAVTGYGDAMVDKTVIMISPEDMAALGAAEGDTISVIRKITWTEQITGA
                     ATKAGGAVKEGATKAGGAVKEGAAKAGQALGKSTKDIAEKVTPKKGDKEL"
     gene            complement(12249..13430)
                     /locus_tag="PK154_02935"
     CDS             complement(12249..13430)
                     /locus_tag="PK154_02935"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HPW10049.1"
                     /translation="MYLIGEALVGDGPELAHIDLLIGDKDGPVGTAFANALSQLSAGH
                     TPLLAVVRPNLLTKPATLVIPKVTLKKDVQIREMFGPVQAAVAKAVADSVEEGVFPDS
                     DIDSLVILASIYLGPGAADYNRIYRYNYGAMKLALRRAMEGFPDRKTLLYEKDRSTHA
                     VMGFKVQRLWDPPYLQVALDLVDMNKVSSVLSELPENDHLIIEAGTPLIKKFGLEIIP
                     EIRKFRPNAFIIADMKILDTGNLEARMAADASADAVVISGLAPTSTLEKAIAEAKKTG
                     IYSVIDMLNVPDPVKVIKGLKVKPDIVELHRAIDAEDTAHAWGNIPALKKAAGGRLLV
                     ATAGGIRVDVVKDAVKAGADIIVVGRAITASKDIHTAADLFLEQLNREEIDQFRVMTD
                     F"
BASE COUNT         2892 a         4087 c         3921 g         2989 t
ORIGIN      
        1 gcagccgcgg ggaccggatc atcgtgaccg agctccccta ccaggtcaac aaggcacagt
       61 ggatcaccgc gatcgccgat atggtcaaag agaagcggat cgacggtatc agcgatatcc
      121 gggacgaatc cgacaaggaa gggatccggg tggtctttga actccggaag ggggcgattg
      181 ccccggtcat cctgaacaac ctctacaagc ataccgcact cgagtcaagt ttctgggcga
      241 tcaactatgc catcgtcgat aaccagccca agatcctcac cctcaccggt ctcctcgagg
      301 aatttgtcag gcaccgggtc caggtgatcc gggcacgatc ggagttcgac ctcaagaaag
      361 cgcaggaacg ggtccatatc cttgacgggc tgctcctggc actcgcacgg atcgacgagg
      421 tcatcgcgac catccgtgct tccgataccg ctgacaccgc ccgggccgcc ctcatcagca
      481 cattcgggct ctctgaacca caggcaactg cgatcctcca gatgcagctt cgccggctgg
      541 ctgcgctcga acagcagaag atactggatg aaaagcacgg gctggagggc gagatcgcga
      601 ggctccagga catcctcgca agcgaggcga acatcagggc tgagatccgt cgcgagactg
      661 atgagatcgc cgccaagttc ggggacgcaa ggaggaccca gatcgcgctt gacatgagcg
      721 aactctcgac cgaggacctg atcgaggaca agacggtgct ggtctccatc acgaccgcaa
      781 actacatcaa gcgtaccgac atcgacagct accggaagca gagaaggggg ggccacggcg
      841 tcaccggcat gaccatcaag gaggatgatg tcgtcgattc cgtctttgtc gccgggatga
      901 aggattacct cctctgtttc accaataccg ggcgggtcta ctggctgaag gtataccaga
      961 tccccgagag ctcccgggtg gcaaaaggca agccgatcgt caacctcctc aacctcaagg
     1021 acgaggtggt gaccacggtc atccctatcc gcgagtttgc agaggatcag catctcctct
     1081 ttgccaccag gctcggccag gtcatcaaga tccccctgga ccagttttcg aatccacgat
     1141 cggtcggcac caatgcgatc aggctgcggg gaggtgacca gctggtcgat gtgatcatga
     1201 cagacgggac aaaggagatc atcctcacca gccggttcgg tcagagcctc cggttccatg
     1261 aggagaccat caggaccgtg gggaggaatg cccagggagt cagggggatg aaactgcggg
     1321 gcggggatac cgtcgttgcc ctgacgcttc ttgagaagga ccacctcctc accctctcgg
     1381 atgtcggttt cggcaagagg accgagtttg acgagttcag ggggcatgga aggggaacga
     1441 tgggagtccg gaacatgcag ctcgaccgtg atgcggtcgt gatcgcttcc tgcgccgtgt
     1501 cccagtccga tgagatcctc gtcatgaccg cgtccggtat cgtcatcagg actcccgtca
     1561 gtgagatacg ggtgatcggt cggggaacaa aaggggtcag gatcatgcgc ctgggggaga
     1621 acgacaaggt cgtcgggatc gcggtcgtcc ctgccgatgt tgacgggaac gatcagaacg
     1681 gggcggacag tgagaaggaa ccgactcccc ctgcctcccc tgaaccatga gcgatcttcc
     1741 ctgacccact tctcttaccg cggctgaatg cgctgataca tcgctccagg tacttcctgc
     1801 cggccttttt ttcggggttt cccgaacggt gatcgggcaa tgcgcagtca ccccttccgt
     1861 tttcatgggt aaggaatgcc tatccttgtt cattcctgcc ggcgataccc tggtggatgg
     1921 cgtgaccccg tatttgtctc gtcatcgcct gcgatccgct ccgcgattgc agggtgccgg
     1981 gctcagtcag gggaggaatg ggggagcact tcaagggatc cccggatccc attactctca
     2041 tatgtaccgg aaagagatcc aggtgacaac gtcacatgaa ggggatatcg tcgatatcac
     2101 tccccaggtg cgggagatcg tccgcgggag cgggatcccc gaaggggtcg cgcatgtgtt
     2161 cgtggcaggg tccactgccg cactcacgac catcgagtac gaatccggcg tccttgccga
     2221 tctctcccgt gccctgtcga ccatcgcgcc ggactcgatc ccctacgccc atgaccgccg
     2281 ctggggggat gggaacggcc gttcgcatgt cagggcatcg atagtcggcc cctccctctc
     2341 cctcccggtg gctgaccatg acctcctgct cgggacctgg cagcaggtgg tcctgctcga
     2401 gctggacgtc aggaagggga gatcacgaac gatgtacgtg tccgtcatcg attaggggag
     2461 accggtccgg tgcgttcctc tccggaagga acggtcctct cctgaaagaa attacctgaa
     2521 gtcgatttga gaattacctg aagtcgattt gagaaccagc aaaaaaagaa aatggcgggg
     2581 ccatcttcat aaaagcgggt attctgcgat cagtccaagg gaattgtctc aagctgggag
     2641 accagctccc agatacgcgt ccgtgcgtga accggcatgt tcgggtcgtt actgatctca
     2701 tcgatcttgg agatggcttc ggctgcccgg agcccagtcc ccttgctctg gttcaaaagg
     2761 agctgacgtg tctcatcggc tacccggcgg atattccggg ggatggtgct gtcatccatg
     2821 atatgttgca gcatcaggat gcagttatcg attgttttct cgggatttgg catagggggc
     2881 cctccactct tctcttatta atacgaatat cccacttata aagtttggta atcgtcattc
     2941 tgtgaccttt ttttagacaa aatgcggttt aatttctttt tttccgtcct caacagtgta
     3001 gggcggacct ttttttggcc ggaggagaaa tcatgatatc tggccacata ccaactatgg
     3061 acaaaggaga atggaggatg cccacccctg tgaagcgcga agatgatatc atggccggat
     3121 acctcctcaa aggggggaag atgctctcca agagctgccc tgcctgcggc tgccccctct
     3181 ttgagatcaa gggagagacg ctctgtgtgg tatgccgtga agatgaagtc agccaacatc
     3241 cccctccccc cgccgctgca ccgctgcctg ttcctgccga tcaggaggtc ccggcgggca
     3301 ctgcaccctc cttcccgtca ctttcggcag cactggagga gacgctcatc gaactctgcc
     3361 ggcgcatccg ggacgagcgg gatcccgccc aggtgctgat cctcgcgaac gcggtgagat
     3421 ccgggattaa agccctgaag cagctccggt agaggctcag gatccggttc agagaacaag
     3481 atctgctccg ctagcgatac tcttttctga ccaggtcagc gatccgcttc gcccttttct
     3541 ccccgatacc cttcaccatt gcgagctcct cggtactggc attggcaatc gcctggatcg
     3601 tcctgaaatg atccaggagg agcctcgcat tcttcagccc gatctcggga aacgacccga
     3661 tgatccgttc ctgctcctcg gtgaccgact ggtatgcctt cctgaacgga agccgcccgg
     3721 tccgctcccc tccctcctcc tcccttcgtg caaggacaat gagcatctgg gccgtctctt
     3781 ccgggtccct ggtgaagaag ggagtgatcc ccaggtcaat ggcaatggcg gcaagggcac
     3841 cgcggagggc attcgggttg acatcccgct cgctgtaaag gtcaccccct tcaaggatca
     3901 ggactggccg gagcgcatgc cctgccaatc ccttgagttg cccaaacagg tcccgctcaa
     3961 tgagcgtatc gacaaagtcc ctggtggtct tccgttccac cacgatgcgg tcccctgcca
     4021 ggtaatcgcc gtaggggagc cgctgcacgg aaagggatgc accaagagaa tgcaggtgtt
     4081 ccaccacctt cgaggaggtc tcccggtcgt ccacgacgat tgcgggacct tgcggcatga
     4141 actcgccgat cctcttctgg atcggccttt cccccacctg ttctgtctgg tcctccccgg
     4201 cccttgcctc catccgggaa cccagcgtcc tgatcccgct gatcatcgca cgctcgcgtg
     4261 actggctgac ccaccggtag gtctcatcag aggtaccctt cgtgaccagc acaacgatcc
     4321 tgccgctccc gctccggccg gtcctgcctt tccgctggat actcctgatc tctgagggga
     4381 ctgcctcgta gaagatcacc aggtccgtgg aggggacatc aagcccctcc tctccgaccg
     4441 aggtagcaac aagcaccggg aattcccctt cccggaacct cctgaggcaa tcgatctgct
     4501 tcttctgcga gagcccgcgg tccgcatctc tcgcagcctg gccgacaaac cgctcggcac
     4561 ggatgccctt cttcccgagg tattccacga tctgttggac catgtcccgg aacgtggcaa
     4621 agatgatgat cctgctcccc ggataccggg tgagctggtc ctgtgtcacc gatgcgagga
     4681 tctccagttt cgggtggagt tctgtttccc aggacatggt ccgttccagg agacggataa
     4741 ataccggatc ctttaccagc cgtgctgccg ccttcgaacc tcctgcagac gagccctcgg
     4801 aaaagagccg gtggatatac ccggcaagta ccacgcttcc ctgggactcg gccagcgaga
     4861 cagcatgccg gagtttcatc agttctgcat acagggatgc ggcagtgtat ccggcggcat
     4921 ccctctgctg tatccgctcc tgaatctggc cattgagggc attgagcact ctcatcgtga
     4981 gctgctgcct cctggggagg tcaaagccga gttcgcggat gagagcgatc cgtgcatcca
     5041 ggagctcgtt cagatccacc accgcggatt gcagcgagag gggaagatca acctcgatca
     5101 cctggatctc ccgttcatgg atgtagggtg ccacatcgct gtcctgttcg gtccgtgtct
     5161 ccacgatcgt caccccgagg ttacggcaga cctcctcgac tttcatctcc tgcccccccg
     5221 gggaggcggt catggcaagg agcagcggtg aacgggccgt ctcccggtag actgaggcaa
     5281 tgaatacgta tgcgtagttc cccaccgcac gatggcattc atcgaccacg aggagggtga
     5341 catcggagag ggtataggcc cccgaaagga gatcgttttt gatcacctgc ggggtggcaa
     5401 agacaagcct ggcacgcgag aactcctccc gccgctggtc aggaggggtc tccccggtaa
     5461 agagggcaca ttcaggatcg ttgccgtcga tcacgataaa ccggcggaag aaccggtagt
     5521 gctgctccac cagcggtttt gtcggcgcca gcatcagcac ttttcccttc tcgttgtaga
     5581 gccgcgaggc cgcgacgata agggcgatcg ccgtcttgcc aaggccggtc ggcagcacca
     5641 ccatcgtatg gccatcgagc gctctcatgg cgatcgagag ctggtacgtc cgggactcga
     5701 gggtcccggt acggatgtac ggatgggtga tccaggacat gggcttcaga gttcggaaaa
     5761 gctcacggtc tgatcgatga gtgaccggac aaggtcaggg atcctcccgg catcgaccct
     5821 gacctgccgc atgctgtccc ggtccctgat cgtgaccgtc cggtcctcct tcgtgtcata
     5881 atcgacggtg atggcaaagg gggtccccac ctcgtcctgc ctccggtaac gcctcccgat
     5941 tgccccggag tcatcgaact ccgcgaggat tcccctcgtc ttcagggcat cggtgatctc
     6001 cagtgcgatc tccggaaggc cgttcctgtt catgagggga aagacggcga cctggatcgg
     6061 tgccacggcc ggcggaatcc ggagtactct ccgctcctcc tcgtctacga tctcctcgtg
     6121 gtagctgtgc tcgagcaccc cgtagatcat ccggtcgatc ccgtacgaag gctcgatcac
     6181 gtggggagtg acctcctctc cagggacctc caccacctct tcgcggacct cgtagagatc
     6241 cggggtgatg agcacctcct tcccgtcgat ggtcagggtg atcccgtcag gcccgggcat
     6301 gcaggaggtg agtgcatcgg cgaccgcctt tgccgatccc ctgaaccgcg gcccaagcgc
     6361 ccccatgtcg gggatgacgc ggacccggtt catacgccgg gggacttcat aggggaggaa
     6421 gacggaaaac cgctgcccgc tctgtgtttc gtgtgccctg aggtcgtaat cggtacgatc
     6481 ggcgatccca acggtctcaa cccagccgaa cctgcccgag aagacctcgg catcccagca
     6541 gtcctccgca taatgggcga gctcatcggg gagatgctgg cggaaccgga gcttgtcggg
     6601 ggagaccccg atccgcacga ggaggtcatg cgtcagggcc aggtagtagg cgacgtattc
     6661 gttggcaacc actcccccgt ccacggcttc tcgcatggtc atcacgaggg gatccgcccc
     6721 ggccatctgc tgctttgcgg aaaagagggg catctcgtat cccgcatagc gctggaaccg
     6781 ggggtggttc ttctgcccgg gatggacgaa gatctctgct tctgcctggg taaactcccg
     6841 gagacggatc atgccctgcc gcggggagat ctcgtttctg aaagacttgc caatctggac
     6901 cgttccgaac gggagcttgt cgcggtaaaa ccggaggagc cgcgaaaaat cggtgaagat
     6961 cccctgtgcc gtctccggcc ggagataccc ggttctctgc gatcccggtc cgatggtggt
     7021 atggaacatg aggttgaatg agaagacctc gaccggacca agcacctctt cgcaggcggg
     7081 gcagggcagg gtggcgattg ctgatgcaag tgcctcgctc ttcatcgccg atgcccccgc
     7141 aattccgcgg ccttcggcaa catggtcggc gcgaaggtac tcgttgcagt gggggcactg
     7201 gcacatcttg tctgaaaagc ctttcacatg ccccgaagcg acatagatct cttcctgtcc
     7261 gatcaccggg cattcgatct cgtaataccc ttcaccgatg atgtagaact tcctccagat
     7321 attttcgata ttgcgtttca tcatcgaccc aagcggcccg tagtcgataa aaccagccac
     7381 ggaaccataa cattcagagg taggccagat gaaacctctc ctttttgcca gatcaattat
     7441 cttctcatag atatcactca aggcaatcac ggctctttat gtatgtcatt gcccggaatc
     7501 aaaaagctgt gtggatcccg gaccatagag tgggagtggt accagtgcgg gcaaccatga
     7561 tgccccccca gagtgcgatt ccaagatcgg caggggtgat ctggctggga gccggtgcac
     7621 aaaaaaaata ccctatctta acgcctattt tagtttaaaa tccttcttat tgaagaatcc
     7681 ggctcagaga gatagtgtta aatacattgg ccggtatgta tttcggtaat tcatgcagga
     7741 cgacaccaga aagactcaac tgctccagct ctttaccaac atttccggga aaaccaagat
     7801 cgttgagccg atgaagaaaa ttcatggcac cctgcgtgac cgtgaagcgg tcgagcggga
     7861 gatcgccctc gtgatgcggg agatccttga acagggcctt tttaaaacca agttacgccc
     7921 catccagctc gcaaaactgg tatctgccta ctatgcggga aagaatgata ccgagatcgc
     7981 ccgtgagctg ggcgatgaga aactgtccaa gaccgttgca cgggcacggg tcaggctgaa
     8041 actgttcagg gatctcgact tcaagatgcc tttcgaccgg gaaaagatgg agtcgctgct
     8101 ctactcggga aagaccatga aggagatcag cgatcacctg ggggtcagtc cctcgacgct
     8161 ccgcgaatac cggcatgtga tcgagcagcg ggacgatgtc accatcgaca tctaccttga
     8221 gcggataaag gacgtcatgg aggatcgtga cctctccgag gtcatgacca agagtgcaac
     8281 caaagacggg cttgaggact ctatcgacat caccgaggca gagatggtgg atgtctccta
     8341 ggagaacatc tcttctcttc gttcccttga atccgcctgt cgtaaaaggg tattcctgga
     8401 aaataccggg attgactatc gtttcatccc gatgacactg atctcctgcc atgatacgct
     8461 ggcgataagc ccccggcagg atttctgtgc cagcaggcct cttgagactt cactgctccg
     8521 gcagggactg gattccttgt catactccct gctggatcac catagtgacc tgctcattca
     8581 tcgaatcgga acccgtaaac gtgcaataga ggtatccccc cgccagggaa tcaaggggat
     8641 tcccttccca ggctccattc gctggactac ccaggactgg cagaatccct cgtcccccca
     8701 ggcacccctc cccggaatat catgggtagg gaatcaatga ggcctccttt ctcatcacag
     8761 acaccactca cccccactat ccctgtcagg attatcaccc cgccccttcc tctttcggat
     8821 accgccccct cccgccacat ttttcaatta gtatcagccc agatactgta gcatggagct
     8881 cacctattat ggacattcct gcatcctcct gaaaggagcc aggacggtgc tggtcgatcc
     8941 ctttttcccc gaagggatga ccccaccccc cgccgatatc gtggccgtca cccatgggca
     9001 tgcggatcac atgggagttg ccgttgatct gaaactgccc accgtggcca tcaacgaggt
     9061 ggcgaaatac ctgaaatcga aggggatccc tgccgagggg atgaacatcg gcggcaccat
     9121 cacggtagag ggggtggcct tccacatggt gcaggcggtc cattcatcat ggattgaaga
     9181 ggcaggcatc gggtgcaatg gcggcggtgc agcagggttc gtgatcggga tggagggaaa
     9241 acgtgtatat catgccgggg acaccgcact cttctcggac atgaagctga tcggcgaact
     9301 ctgccacccg gatgttgccc tgctcccgat tggcggtagg ttcaccatgg gccccgagga
     9361 ggcgatgatt gcggcccagt acatcggtgc cccattggtc atccccatcc actacgatac
     9421 ctggccggta atccggcagg atcccggact gttcagggct gccctccaac ggaccaccga
     9481 tatcctggtg aacatacttg ctccgggtga gagcctcacc atttgatgcg aaaaaccgaa
     9541 aatctccatt tttttaatga aaatcacttc gtaattttca taatttatgc ccgtatcccg
     9601 aaatcggggc tcataggagg tttatatgaa aatgcctctg gcattttcat cttttatgct
     9661 tgtggccctc aaggaaatca tgttatttca ttgggaccct gttgtggtcg tgcgcctccc
     9721 cttccgcggt gaaacaatgt tccagcagga gatcccgctg acgagggaaa agggaccggt
     9781 ccacaggaat gaagcccccc ctgacctgat tacctgctat tgaaacaaga gggaggtttg
     9841 ccctggcagg cgggtttttt tgcccacacc acctcttcaa gaagatttca cctcgttatt
     9901 gcctttcagg ggaggtggta ccttccagcc gttctgccag gtctgaaagg ggcaggccac
     9961 ggataaaaat gaagtgtact ccctcagcag agatgacttc cctcatcaga gatgaattcc
    10021 gggtatcccc ggttattctt ttgccctgcc cttctcctgc ccctggagga ggtgcagttt
    10081 cagaatgcca ttgcaggaat tgcagatgta gtagtcctgt gtccagcagg tggagcaggc
    10141 ccacctcccg cacagggtgc actgcttcag cccggtggaa ggatacgtgc tgttgcagag
    10201 gtcgcagtgg aaccgggaga aagaggatgc atctttctca gggggctcac cctgctttct
    10261 catcacggtg acgtcattcc ggacgcggga gaacaggtcg ggatcctttc gggacccctt
    10321 cgtctcactt cttccaggtc tttttttcca gagcatccag ccgctcctcg agcgttttca
    10381 ggctgctttc catcgtctca gaaaaatcct tcagcgaggc aaccttctcc tcgtttgcca
    10441 ggaggacctg gtccttggac tgcctgaatg actggagtct tgcacggctg acaagggacc
    10501 actcctcgat catcagctcg aacctgcgat cgagatactc gtccaccctg ctctccatcg
    10561 gccggctgag cgacacctcc ccttttgccc ccctccagta atagtagatc aggaacagga
    10621 ggatggccac gacgatgata atgagaaccg cctcgagggg agtcatcgca ttgccccctt
    10681 cagctgcagc agccggtgtt caaggttctc cagcgtaatt tcagcactgg tctcgaacga
    10741 ctcgatctcc tttgttgcct cctcgataga atggagccgg ttctcgaaat cgccgatgtc
    10801 attccgtgtc gcaagatccc agtcctggat gaggttcttc atcctccggt ccaggtaggc
    10861 gttcatgtag ctgtccatgc cgaccattat ccgacctcca gctccctgat tcctgcctca
    10921 agggcggcaa cccgcttctc tatctcgtcc ctgccccgtt caaggttccg gatctcggtc
    10981 gtcaccatgt ccagccggtc gtcgagatca tcaagggtat gggtggtgat gagcccccat
    11041 tcgttgatga tcgctgacat gtgggcatcg aaataccggt cgagcccttt gtccatcgta
    11101 ggaagtcgtg actcaatcga tccgggtatc gctgccagag aatcccggaa cgaggatttt
    11161 ttagccgttt caaaaggtgc ctgctcaaac atgatgatca ccctattctt ctctcctcgt
    11221 ccctgatctt ctcgaccatc cggtcgagct tggtcagttt caccttgttc tcgagccgct
    11281 cgatccgctt gtcaaccatg ctgtgtttcg cgaagatatc ggttgcaagg cctgcattct
    11341 catcgtcaag tacctggatc tccttcatct gctcgggtga gacacggaag aatggggtcc
    11401 cacgctgggc catctcggcc ttcatcaggt tgagtttctc cttctcaagg tcaatctcgg
    11461 caaggcgccg ggtattggtc atcagccgga tctccttgat gatgaagtag gcgataataa
    11521 ggacgaggac caggacgagt atccagaaca ccgattcgaa ccatcgccag taaggagagg
    11581 ctgcagcaac tatcaattcc tctgccatcg gacatctctc cggctgttac agttccttgt
    11641 cacccttttt cggagtgact ttctctgcta tgtccttcgt gctcttcccg agggcttggc
    11701 ccgccttggc tgcaccctcc ttaaccgctc ctcctgcctt ggttgcaccc tccttgactg
    11761 ctcctcctgc ctttgttgca gccccggtta tctgttccgt ccacgtgatc ttccggatga
    11821 cggatatggt gtcgccctcc gcggcaccaa gggctgccat atcttcaggg gagatcatga
    11881 tgactgtttt atccaccatc gcatcgccgt agcccgtgac agccacggtc tttgtcttct
    11941 tgtcccagac ggtgggatat tgtgcgagga cgagcgcctc cccctccttc gcaccgatca
    12001 tcggcaggac tgattcatgg acacgggccc ggcctttgct cggaaacgtc cgtaccttca
    12061 ccttcaatgt cactgatcct tccatagcag tcaagggtag cttgttaaac tattaatgct
    12121 ttattaagcc cccggcggga acgttgaaat agaccggaga aaccagtaat tcgcccatgc
    12181 agtccggatc gctggagaaa aaagagtatc aggtggatga ggggtagttt aatggacggg
    12241 aaaaaaaatc agaaatcggt cataaccctg aactggtcga tctcctcgcg attcagctgc
    12301 tcgaggaaga gatccgcagc ggtatggata tccttgcttg cagtgatcgc ccggcccact
    12361 acgatgatat ctgccccggc cttcaccgca tccttcacga catcgaccct gatacctccg
    12421 gcagtggcga cgaggagccg cccccctgca gccttcttaa gagcagggat attgccccag
    12481 gcatgggcag tgtcctcggc atcgatcgcc cggtggagtt ccacgatatc gggcttgacc
    12541 ttgaggccct ttatgacctt gaccgggtcg ggcacattca gcatgtcgat caccgagtag
    12601 atcccggtct tcttcgcctc ggcgatggcc ttctcgagcg ttgaggtggg tgcaagcccc
    12661 gagatgacca cggcatcggc agaggcatct gccgccatcc gggcctcaag gttcccggta
    12721 tcgaggatct tcatgtccgc gatgataaag gcattcgggc ggaacttcct gatctccggg
    12781 atgatctcaa gcccgaactt cttgatgagc ggggtccctg cctcaatgat aaggtggtca
    12841 ttttcaggga gctcggaaag aacagaggag accttgttca tgtcaacaag atcgagtgcc
    12901 acctggagat agggcgggtc ccagagccgc tggaccttga agcccatgac cgcatgggtc
    12961 gaccggtcct tctcgtagag gagtgtcttt ctgtccggga atccctccat ggccctccgg
    13021 agtgccagtt tcatggcgcc atagttgtac cggtagatcc ggttgtagtc tgccgccccg
    13081 ggaccaaggt agatgctcgc caggatcacc agcgagtcga tgtcactgtc cgggaatact
    13141 ccctcctcga ccgagtcggc gaccgctttt gccaccgcgg cctggaccgg gccgaacatc
    13201 tcccggatct ggacatcttt cttcagcgtg accttgggga tgaccagcgt cgccggtttt
    13261 gtgaggaggt ttggccggac cacggcaagg agcggcgtgt ggccggcaga aagctgggag
    13321 agggcgtttg caaacgccgt cccgacaggc ccgtctttgt cgccgatcag aaggtcgata
    13381 tgggcaagtt caggaccatc gcctacgagt gcttcaccga taagatacat gggataccct
    13441 tttcctgcct actaggtttg ggatagtggt ttaaaaagcc ctctggtatc cggggcacac
    13501 acggtcagag cggcagggaa ttgcccccta cagagcggaa tgacgcggga agggagggaa
    13561 atgatgcggg agaggcactg gatgaaaggg atggactgtc agaatctcca aagaagggga
    13621 tcggtgagca ggatgataga gggagcaatg ccgggacgcg ggaaatatca ctcctttaaa
    13681 gagcggatgt ccgggtaaag gggatacccc tggaatacct gaaaggaata taccggagcg
    13741 tgcctgggat ccgggatggt ttgggctgga gaagaacgga aagcgccagg agaatgcctg
    13801 gggatcagaa cagcagggtc agaggaatgg gaaaccgtgc aatgcggaac aggaaaatat
    13861 ggggtcggtg agatttgaac tcacgatcg
//
LOCUS       DAOOCO010000029        13835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4047_length_13835_cov_4.916473, whole genome shotgun sequence.
ACCESSION   DAOOCO010000029 DAOOCO010000000
VERSION     DAOOCO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4047_length_13835_cov_4.916473"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1276
                     /locus_tag="PK154_02940"
     CDS             239..1276
                     /locus_tag="PK154_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HPW10050.1"
                     /translation="MSTPSHPCILVLHGPEVFDAGDVHYLIDRLHPERVIVAGVMART
                     AAEESGIDCEFISVPPSLVLKDLSGCTLLANHGKTPESGRIFGEIVASRLFHRGLIHL
                     ECSDKTLYSWNHAIDNLVHDIGEKTGFRIVPQKISKNEPSPKRSIRGCVRGEPVYVNG
                     IVIGHATGPEVILTLEEGTIIPESGIIPKMHGLEKIAKIPIHDLSGIWCKSGTIRRNA
                     PVPTNLRQRQQGRVLFIDHAGHHLYRLLTPDICGIISVGDDTTAVCGHIGSHLGIPVF
                     GIIDGDRDGIVEAGYAPGSVLARAVRERDDDIGTEIRAMIPQGQVTWNDLVSQLILHL
                     GSRVAISRYPD"
     gene            1283..2425
                     /locus_tag="PK154_02945"
     CDS             1283..2425
                     /locus_tag="PK154_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10051.1"
                     /translation="MLCAVCKGKGLCGLSRCPIMERFHSLATVRPVTEYQGTAPSVFI
                     GSAGYPSVSGGPLLVQDTDIPTDWLSQGLGIEDIVSLRSETIRGISKVAPYLGQIQDI
                     ALASRPISVEASFEQPVRFEPDFDRVTAPVGSVGNIRRLDLLDHPPTDRAVDRIVSDT
                     DLPAAEACIDLHRSGLDVYRITPLFTAGLLGKKRRIVPTRWAITAVDDSVSTAQKHAV
                     ARFSQLQEIRLHYSELFGNRIAVILVPGDWRYEMIERWEPRSLWAGETATVVRDGETM
                     KKTGYSPITGAYYSARLAVTEHLFRSGRCARVIVIRNVDPTYWAPLGTWVVREATRRA
                     MAGSPLVVSSLTDAIQQAGSLAGFPDWIHHSALVPEICFQKTLSEF"
     gene            complement(2493..4136)
                     /locus_tag="PK154_02950"
     CDS             complement(2493..4136)
                     /locus_tag="PK154_02950"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPW10052.1"
                     /translation="MNFREFARVCEELEGIPGRLAMIDLISRILPGLTEEELPVFIRF
                     IMGRIFPDWSPEKLGIGPNLLYEGIAYVAGIRKEEVIRIINRTGDVGRAVEELLGRKE
                     QTSFFTEELSLLDVYRELAAIAATGGNRSQKEKLKVVRKLFSNAGPLEGRYLARIMLE
                     DLRIGVGEGNVREAIARAFQVDPYQVEHAFQAINDLGQVALLAKEGEERLKEVRIALF
                     HPVKMMLAQQGSIEGAVAENGAMAAEYKYDGSRFQFHKSGATCRMYSRKLEEVTDALP
                     DVVDLLAKSTDHDVILDGEVIAIRDDRPLPFQTVLRRFRRKYGIDEIREEIRMIPNVF
                     DLLYLDGETLIDLPFLERRIRLMGVINAYIAPQIVSGDPAVIDRMYQEALASGHEGLM
                     LKVPSSPYTPGVRGKNWIKIKPAVDSLDLAVIGADWGEGKRAHLFGSFLLACQDANGE
                     LVPLSKVATGFSDEQLQEMYALLKDRVISESGKELRFEPGIVIEVGYSEIQKSPNYEG
                     GFALRFPRFIRLRDDKSVDEIETLESIRERHLKQSAGQQ"
     gene            complement(4282..5070)
                     /locus_tag="PK154_02955"
     CDS             complement(4282..5070)
                     /locus_tag="PK154_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW10053.1"
                     /translation="MDCIALASGSSGNCLYIESDDSALLIDAGLSKKEILARLNTAGG
                     KSDRVQAILVTHEHIDHMRGVYALARSLKVPVCATGGTLHEFIRCRGSTIMPVELVRC
                     RFDERLEIGDFSIEAYPTSHDAKEPCAFRVEEGSISVCCCTDTGTISPGLQEWLARCD
                     ALVLESNHCPEMLKTGPYPEMLKRRIRSKRGHLSNPDAAACIRTLSQQIHTVMLSHLS
                     EVNNTPEKAMKTAQEGIGLFMDEIEITLASNPDKDQHWPGRIRV"
     gene            complement(5128..6222)
                     /locus_tag="PK154_02960"
     CDS             complement(5128..6222)
                     /locus_tag="PK154_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10054.1"
                     /translation="MIEFEHVVMNFGEKEVLKDNSFTIQKGEIFTFIGPSGSGKTTIL
                     RLIDMLERPTSGTISIDGTIVSSLKDRDRIPLRRKMSMVFQKPSPLRGTVFDNVAIGL
                     LFRSINHDEIKARVPEALELVGLKGYEDRKAGTLSGGEMQRVAIARAISTKPDILLLD
                     EPTANLDPVSTEKIENLIFSLKERFGITVVLSTHDMVQGQRLADRIAVIIDGRIGQIG
                     TSKEIFYHPMNRAVANMVGVENIFEGVIVKNEGGLASIDVDGTSIIATTAFPPGTPVV
                     LYMRPEDITFHVRDGVKSSARNEFTGTITKVLPMGPNARLKVDAGIKLTAVITQRSYE
                     ELDLALGKETQLAFKASAIHVVVRKPLKSV"
     gene            complement(6227..6913)
                     /locus_tag="PK154_02965"
     CDS             complement(6227..6913)
                     /locus_tag="PK154_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10055.1"
                     /translation="MNEVIEGIIQAVQLIVSLDEEVLEITARSLLISLSATLIGSAIA
                     IPIGGFIHFKEFGGKRGLVTIIQALYALPTVLAGLLVFLLLSRSGPFGFLRLMFTPTG
                     MVIGQIVLIIPLMIGMTLIALSGVSRTKQDTVISLGASETQTVITIVKEARFAILGGV
                     ILGFGRAISEVGAAMMIGGNIRGYTRVLTTAIALETSMGNLSLSIALGLILLAIATVV
                     TGILFLVQER"
     gene            complement(7115..7462)
                     /locus_tag="PK154_02970"
     CDS             complement(7115..7462)
                     /locus_tag="PK154_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10056.1"
                     /translation="MATLSIEIIYDAVLRIQLFEEHEISVIFSEKGVRIKLPTTRRLA
                     EFLAVPHYYVLPYFAMMEQESLVTRAERVGIMTTPTGTARYLALMKKSYPAEGEAILG
                     PDVYKELMNRAGL"
     gene            7794..8591
                     /gene="fdhD"
                     /locus_tag="PK154_02975"
     CDS             7794..8591
                     /gene="fdhD"
                     /locus_tag="PK154_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPW10057.1"
                     /translation="MGSQDDTAMNMKKTMTLDHEILAVSDDTVERKTACVCIEDTVTV
                     TLNGIPVTTLKITPVDLDSFAIGFLICEGLISDPDCITSIRIDLPVIAVTAEHCTTEG
                     NAPSLEIRSAGVGIRHRQELSGPPIGRGIVIDKSVVFEGTRQVHETATVWRSTGGTHC
                     TILVDSEGHVRSAAEDIGRHTSVDKAVGKALLSGVNPGHTFMVCTGRLPADMVAKAYR
                     AGIPIVVSNNAPFSSGISLAERMNMTLVGFARPPRMSIYTHPERIRL"
     gene            8874..9050
                     /locus_tag="PK154_02980"
     CDS             8874..9050
                     /locus_tag="PK154_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10058.1"
                     /translation="MDEQKIQSIFREAGIEKEIKCPEAFLISEKYGISKTDISRFCNE
                     NGIKIRACQLGCFK"
     gene            9270..9659
                     /locus_tag="PK154_02985"
     CDS             9270..9659
                     /locus_tag="PK154_02985"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPW10059.1"
                     /translation="MSQTKKILIEWRHLEFGEIPCGRCTDTGTNLLKVITQLGDEDLL
                     NNVELEIQNTILSPDKVDESNVLIINGIQLEHLLGTGISFSGCSCRTDISKDQVSCKA
                     VQLDRDVFKAIPEEMIRGALLEVLKQD"
     gene            9806..10015
                     /locus_tag="PK154_02990"
     CDS             9806..10015
                     /locus_tag="PK154_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPW10060.1"
                     /translation="MKISARNQIPGTVKLLKKGPVTTEVVISVGAGQEIVSTITTHSA
                     ETMKLREGSKVYAVMKASEVMVGID"
     gene            complement(10042..10326)
                     /locus_tag="PK154_02995"
     CDS             complement(10042..10326)
                     /locus_tag="PK154_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10061.1"
                     /translation="MSTPAAHQVSPYPDWELRIYQLTRPVNRYDIQAFLGDQDAYIRD
                     TAAGPVHTIHKYGLLEIHARVGEKGIEVWYNPEQGSYPLEYFEALLATRF"
     gene            10572..11381
                     /gene="modA"
                     /locus_tag="PK154_03000"
     CDS             10572..11381
                     /gene="modA"
                     /locus_tag="PK154_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW10062.1"
                     /translation="MKFSWLTTISLVLLAAIIVAGCTSEQAQPEQKNDLTVFAAASLT
                     GAITEMAKEYETSHSDIHVITNFDGSQALRTQIEQGAYADVFLSANTKHLYALRDQGL
                     MENSSIGLFAKNRLAVITPASNPGNIMTLDDLARPGTRLVIGTKDVPVGDYARQILTK
                     MGNDTAYGPSYNSSVRANVISEETTVTGVVAKVQLGEADAGIVYESDITPENRNKFTV
                     IAIPDTFNVIAEYPAGVVAASQHKEEAAAFLAYLNGELGRATLAKYGFALP"
     gene            11396..12208
                     /locus_tag="PK154_03005"
     CDS             11396..12208
                     /locus_tag="PK154_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10063.1"
                     /translation="MMGRRYFKGRNNLLTIGAGVLITLLVIFIITPILGLFSRITFDQ
                     FIASLSDPVVWNALVLSLFTAGISTFVIILVGTPVAWINARHHYPGREIVDTLIDLPL
                     VLPPTVAGLALLMAFGRMGLIGRFFYNFGISIAFTTLAVIIAQIFVSVPFYIRQARAS
                     FEQLDAMYENAARSLGSPPVPTFFTVVLPLVKAGLIGGAIMSFARALGEFGATIMFAG
                     NFQGRTQTMPLAIYTTMQGDLNFSITLSVILIIISFIVIAVVKFKLRGYTPR"
     gene            12229..12936
                     /locus_tag="PK154_03010"
     CDS             12229..12936
                     /locus_tag="PK154_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10064.1"
                     /translation="MRDFPLTAEFSVRDGETLVLIGENGSGKSSLLYLLSGLLAPESG
                     FISLDNRLLYEGSKGTSIPSEERDIGFVFQNYAIFPHLTVFENIAFGLRRRKVPDSEL
                     RSRVEEYLSLLSLDTLSSIRGDQLSGGERQRAALARALVIEPSLLLLDEPVSALDAVA
                     QENMRSQLRTYVRKSDIPCIAVTHSFRDAVSLGDRLGVMERGRIVQNGALEEIKGSGN
                     TFLHHFFCGCSKDSCRR"
     gene            13001..13387
                     /locus_tag="PK154_03015"
     CDS             13001..13387
                     /locus_tag="PK154_03015"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPW10065.1"
                     /translation="MTGKIPIIIEWRHLDLGQPFSGHCSETGATLWEVITTLGQEHWL
                     DDIGLELKNTILLPEETEESNTVLVNGIPVEQILNTGDTFGCSGGQDTGSEVTPLQRV
                     PPGREVLKGIPYEVLRKAILTVLTRT"
     gene            complement(13687..>13835)
                     /locus_tag="PK154_03020"
     CDS             complement(13687..>13835)
                     /locus_tag="PK154_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW10066.1"
                     /translation="QMYTQLAKLAKDTETQFLFSQLANMERGHKARLEDIYTNMAFPE
                     NVWV"
BASE COUNT         3225 a         3673 c         3576 g         3361 t
ORIGIN      
        1 ctttctctgt tctgttcgat aaaatggtaa ttgaagaact gaatcgattg ttcggatttg
       61 aatgagggaa atgtccctga tatgattttt aatggttgta acggttcagg aagggatgag
      121 cttattgatg agtcgtcagc ttgctatgaa ttgagggaaa ttctgaggga gtgctcctcc
      181 atccgcaccc ttatctgaaa tctcaaccta tctttttcaa gacaggaata atcgtcctat
      241 gtccacccct tctcatccct gtatcctggt cctccacgga ccggaggtat ttgatgccgg
      301 cgatgttcat tatcttattg accggctcca tccggaacgg gtgattgttg cgggggtgat
      361 ggcccggacc gccgcagagg agtccgggat agactgcgag ttcatcagcg tgcctcccag
      421 cctcgttctg aaagatctct ccggctgcac tcttctcgcc aaccacggca agacccctga
      481 gtccgggcga atattcggtg agattgtggc atctcgtctt ttccaccggg ggctcatcca
      541 ccttgaatgc tcagacaaga ccctttattc atggaaccat gctattgaca atctggtcca
      601 tgacattggg gaaaagaccg gttttcgtat tgttccgcag aagatctcaa aaaacgaacc
      661 atcaccgaaa cggtcgattc gggggtgcgt caggggggaa cctgtctatg tcaacggcat
      721 agtgattggt catgccacgg gacccgaggt cattctcaca ctcgaggaag gaaccattat
      781 tcccgaatcg gggatcatac cgaaaatgca cggtcttgag aagatcgcaa agataccgat
      841 acatgacctt tccggtatct ggtgcaagag cgggacgata cggagaaatg ctccggttcc
      901 caccaatctg cggcagaggc agcagggccg ggtacttttc atcgatcacg ccgggcatca
      961 cctgtaccgc ctgctcactc ctgacatctg cgggataatc agcgtcggcg atgatacaac
     1021 ggcagtatgc gggcatatcg gctcccatct gggtatccct gtcttcggca ttattgacgg
     1081 agaccgtgac ggaatagtcg aagcagggta cgcccctggt tctgttctcg caagagcagt
     1141 acgcgagcgg gatgatgaca ttggtaccga gatcagggcg atgatcccgc aagggcaggt
     1201 gacgtggaat gacctggtgt cacagctcat ccttcatctt ggaagcaggg tggctatctc
     1261 ccgatacccg gattgattct ctatgctctg cgctgtatgc aagggaaaag gtctttgtgg
     1321 actctcaagg tgtcccatca tggagcgttt ccactcactg gccacagtac gtccggttac
     1381 tgagtatcaa gggacagccc cgtctgtctt tatcggtagt gcagggtacc cttcagtcag
     1441 tggcgggcct ctcctcgtcc aggacaccga cattccgacc gactggttat cacagggtct
     1501 cgggatcgag gatatcgtca gcctccggtc agagaccatc aggggaattt caaaggttgc
     1561 tccctatctt gggcagatac aggatatcgc gcttgcctca cgccccattt cggtcgaagc
     1621 ctccttcgaa caaccagtca ggttcgaacc tgattttgac cgggtgactg cacccgttgg
     1681 aagcgtgggg aatatccggc gacttgatct gcttgatcat ccccctaccg atcgtgcggt
     1741 ggatcggatt gtctcagata ccgacctccc tgcggcagag gcatgcatcg atcttcaccg
     1801 gtcgggactt gatgtatacc ggatcacccc tctctttact gcggggcttc ttgggaagaa
     1861 aagaaggatc gtacccaccc ggtgggcaat aacagcagtc gatgattctg tctcaaccgc
     1921 acagaaacat gctgttgcgc ggttctctca acttcaggag atccggctcc attattcgga
     1981 actgtttgga aaccggatcg ctgtgatcct cgtacccggt gactggcgat acgagatgat
     2041 cgagagatgg gagccgcgaa gcctctgggc aggtgagact gcaaccgttg tcagggacgg
     2101 agaaaccatg aaaaaaacag gatactcccc gataaccggt gcgtactatt cggcaaggct
     2161 tgcggtcacc gagcacctgt tccgatcagg tcggtgtgcc cgcgtgatcg tgatccggaa
     2221 tgttgacccc acctactggg cgccgctcgg aacatgggtt gtgcgggagg cgacacgccg
     2281 tgcaatggcg gggtcaccac ttgttgtttc ttctcttact gatgccatcc agcaggcagg
     2341 ctctcttgct ggttttcctg actggatcca tcactctgcc cttgtcccgg agatctgttt
     2401 ccagaagaca ctctctgagt tttaggcagt ggccttcctg taaaggaggt gcagggaacc
     2461 tcgataatgg gggagagggt tccccgggat gctcactgct gtcccgctga ttgcttcaaa
     2521 tgccgttccc tgatgctctc aagggtctcg atctcatcga ccgacttgtc atcgcggagg
     2581 cggatgaacc ggggaaaccg caacgcaaat cctccctcgt aattgggact tttctggatc
     2641 tcggagtaac ctacctcgat cacgatcccc ggctcgaacc gcagctcttt ccctgactcg
     2701 ctgatcaccc tgtctttgag gagggcatac atctcctgga gctgctcatc ggaaaagcct
     2761 gttgccacct tcgataacgg gaccagttcc ccgtttgcat cctggcatgc gaggagaaaa
     2821 gagccgaaga gatgtgcccg tttcccttca ccccagtcag caccaatcac cgcgaggtcc
     2881 agggaatcaa ccgctggttt tatcttgatc cagttcttcc ccctcactcc cggcgtgtag
     2941 ggcgaagacg ggactttcag catcagccct tcgtggcctg aggccagtgc ttcctggtac
     3001 atccgatcga tcaccgctgg atccccgctc acgatctggg gagcaatata ggcattgatc
     3061 actcccatga gccgtattct ccgttccaag aaggggaggt ctatgagggt ctcgccatcg
     3121 aggtagagga ggtcaaagac gttcgggatc atcctgatct cctcgcgtat ctcatcgatc
     3181 ccgtattttc tccggaacct ccgaagaaca gtctggaatg gcagaggacg atcatccctg
     3241 atggcgataa cctctccgtc gaggatcaca tcatggtcgg tagactttgc caggagatcg
     3301 acgacgtccg ggagtgcatc ggtgacctcc tccagtttcc gcgagtacat cctgcaggtg
     3361 gcgcccgatt tatgaaactg gaaccttgat ccatcgtatt tgtattctgc ggccattgcc
     3421 ccgttctcgg caacagctcc ctcgatactt ccctgctggg caagcatcat cttcactggg
     3481 tggaaaaggg caatccgcac ctccttcaac cgttcctccc cctcttttgc gaggagtgca
     3541 acctgcccaa ggtcattgat tgcctggaag gcatgctcga cctggtaagg atcgacctgg
     3601 aaggcccggg cgattgcttc cctgacattc ccttctccaa ctcctatcct caaatcctca
     3661 agcatgatcc gggcaagata tctcccttcg aggggacccg cattgctgaa cagcttcctg
     3721 acgactttca gtttctcttt ctgcgaccgg ttcccgccag tggcagcgat ggcagcaagt
     3781 tccctgtata catcaagaag agagagttcc tcggtgaaga acgaagtctg ttctttcctt
     3841 cccaggagtt cctcgaccgc tctccccaca tccccggtcc ggttgattat ccggatgacc
     3901 tcctctttcc gtattcctgc cacgtatgcg atcccctcat agaggagatt tggccctatc
     3961 ccgagttttt cagggctcca gtcaggaaag atccgtccca taatgaacct gatgaatacc
     4021 ggcagttctt cctcggtgag cccgggaagg atccggctga tcagatcgat catcgcaagg
     4081 cgtccgggga tcccttcaag ttcctcgcag acacgtgcaa attcacgaaa gttcatgtac
     4141 aggccctttt cgcatcaggt ttggtggaac agggaataag gattgttatg tcgttcctgc
     4201 cgagggtcca agcctcacct gataagcgaa aaatccccga atgaccggag agcgtcatta
     4261 ctaccaggag acgaatcaac atcagaccct gatccgtccg ggccagtgct ggtctttatc
     4321 cggattggag gcaagggtga tctcgatctc gtccatgaag aggccgatcc cttcctgtgc
     4381 ggtcttcatg gctttttcag gagtgttatt cacctccgaa aggtgagaga gcatcaccgt
     4441 atggatctgc tgggagaggg tgcggatgca tgctgcagca tcaggattgg agagatgccc
     4501 ccgctttgac cggattcttc gtttcagcat ctccgggtaa ggaccggtct tgagcatctc
     4561 cgggcagtgg ttgctctcaa ggaccagtgc gtcacagcgg gcaagccact cctggagccc
     4621 gggagagatg gttccggtat cggtacagca acatactgaa atgctgccct cttctacgcg
     4681 gaatgcacag ggctcctttg catcgtggga cgttggatat gcttcaattg aaaaatcccc
     4741 gatctccaga cgctcatcaa aacggcaccg taccagttcg accggcatga ttgtactgcc
     4801 cctgcatcgt atgaattcat gaagagttcc ccccgtggca cataccggga ccttcaggga
     4861 gcgggcaagg gcgtatactc ctctcatgtg atcgatatgt tcgtgggtta ccagtattgc
     4921 ctggaccctg tcagatttgc cccctgcagt attcagccgg gccaggatct ctttcttcga
     4981 aagaccggca tcgatgagca gcgctgagtc atcgctctca atatagaggc agttcccgct
     5041 gctcccgctt gcaagggcga tgcaatccat tctgtaagag cataaggcat gatccaatat
     5101 acaggcgtcg attctggaag ggagcaatta aactgatttt aagggcttcc tcaccacgac
     5161 atggatcgcg ctcgccttga aagccagctg tgtctccttc ccgagtgcaa ggtcaagttc
     5221 ttcgtatgat cgctgggtga taacggcggt aagtttgatc cctgcatcga ccttcaacct
     5281 tgcattcggc cccattggga ggacctttgt gatcgttccg gtgaattcgt tccgggcact
     5341 gctcttcacc ccatccctga catggaacgt gatatcttca gggcgcatgt agagcaccac
     5401 cggcgtcccg ggaggaaacg cggttgttgc gatgatggaa gtgccgtcaa catcgataga
     5461 agcgagccct ccctcgtttt tgacgatgac cccctcaaag atattctcca cccctaccat
     5521 gttcgccact gcccggttca tcgggtggta gaagatctcc tttgaggtcc cgatctggcc
     5581 gatcctgcca tcgatgatca ccgcaatgcg gtctgcaagc ctctgcccct gcaccatgtc
     5641 atgagtcgag aggacgacgg tgattccgaa ccgttccttg agcgagaaga tgaggttctc
     5701 gatcttctcg gtggagaccg ggtcaaggtt cgcagtgggt tcatccagga gcaggatatc
     5761 cggtttcgtg gagatcgccc gggcgatagc cacccgctgc atctccccac cggagagggt
     5821 ccctgctttc cgatcttcgt agcctttgag ccctaccagc tcgagcgctt ccggcaccct
     5881 ggcctttatc tcgtcatggt tgatgctcct gaataaaagc cctatagcga cattgtcaaa
     5941 aacggttcca cgaaggggtg acggtttctg gaacaccatg ctcatcttcc ggcggagggg
     6001 gatacggtcc cggtctttca gggaagagac aatggtaccg tcaattgata tcgttccgct
     6061 ggtaggccgt tccagcatgt ctatcaggcg gaggatcgtg gtcttcccgg agccacttgg
     6121 cccgataaag gtgaagatct cgcccttctg tatggtaaat gaattatcct tcaatacttc
     6181 tttttcgccg aaattcatca ccacgtgctc gaattcgatc atgatctcac cgctcctgga
     6241 caaggaagag tatcccggtc acaaccgtag caattgccag caggatcagc cccagcgcaa
     6301 tggagaggga gaggttcccc atcgatgtct caagggcgat ggcggtggtg agaacccggg
     6361 tgtatcccct gatattgcca ccgatcatca tcgcagcccc tacctcggag atcgcccggc
     6421 cgaaccccag gatcactccg ccaagaatgg caaaccgcgc ctccttgacg atggtgatca
     6481 cagtctgggt ctcagaggca ccgagcgaaa taacggtatc ctgtttggtc cggctcactc
     6541 ctgacagagc gatcagggtc atcccaatca tcaatggaat aatcagaaca atctggccga
     6601 tgaccattcc ggtcggcgtg aacatcagtc tgagaaatcc gaacggaccg gaacgggaaa
     6661 ggagcaggaa gaccaggagg ccagccagca ccgtcggaag tgcatagagg gcctggatga
     6721 tggtgacaag ccctctcttt ccgccaaatt ccttgaaatg gatgaatccc ccgataggga
     6781 tagcaatcgc cgaaccgata agggttgccg agagcgaaat caggagagac cgtgccgtga
     6841 tctcaagaac ttcctcatca agggatacaa tcagttgaac cgcctggatg attccttcaa
     6901 taacttcatt catgcaggga tactcctcaa gtggtcatag gggccgcctc acggctatga
     6961 tgaaataaag tgccagttca cggtcctgaa attaccctat cagtaaccag gacaattctg
     7021 tgaagggtat ttcaataagt atctaattgg attctggcag aatattaatg atatccgttt
     7081 acttgctgct acatcaactg gtatagtagt tgaatcagag cccagccctg ttcatgagtt
     7141 ccttatacac atccggacca agaattgcct ctccttctgc cgggtacgac tttttcatca
     7201 atgcaagata tcgcgcggtc ccggtaggag tggtcataat cccgacccgt tctgcacggg
     7261 tgacaaggga ctcctgctcc atcatggcaa aatacgggag aacgtagtaa tgaggaacag
     7321 cgaggaactc tgcaagtctc cgggtggttg gtaatttgat tcgaaccccc ttctcggaaa
     7381 agatgacgga tatctcgtgt tcctcaaaga gctggatgcg gagcacggca tcatagatga
     7441 tttcaataga gagtgttgcc atcggaaccg tagtatactc atcgtcactc aggcacaaaa
     7501 aggatagggt gatcctaagc cagtgcaaca ataagatgca tcattgcgac cgtgtatctc
     7561 tgctatcggt gcgatttatt aagtaaaaat gatgaataac caaaacagat aagtgaactt
     7621 aattattatc tctcaccatt cttattgttc ggacctgtat cccggtactg ttaccccctc
     7681 agtcaggaaa tgacagggta tgtgccttca ctgccctcct ggaaccgggt tatcactggt
     7741 ctctgaacat ttcagcccgg atctcaaata ggtacgcttg aggtgactgt tggatgggct
     7801 cgcaagacga cacggcaatg aatatgaaaa aaaccatgac ccttgaccac gagattctcg
     7861 cagtcagcga tgatactgtg gagaggaaaa cggcatgcgt ctgtattgaa gataccgtca
     7921 ccgtcaccct caacgggata ccggtgacca cccttaaaat cactcctgtg gatctggata
     7981 gctttgcaat cggctttctc atctgcgaag ggctgatcag cgatcctgat tgtatcacat
     8041 caatacggat cgatctcccc gttatcgctg ttactgcaga gcattgtaca acggaaggga
     8101 atgctccctc gcttgagatt cggagtgcag gtgtgggtat tcggcaccgg caggaactat
     8161 ccggacctcc tatcgggagg ggtatcgtca ttgataaaag cgttgtattt gaagggacac
     8221 gacaggtcca cgagaccgct accgtatggc ggtcaacagg gggaacccac tgtaccatcc
     8281 tggttgattc agaaggtcat gtcaggtctg ccgctgagga tatcgggcgc catacttcag
     8341 tagacaaggc ggtgggaaaa gcgctcctgt ccggggtaaa tcccggccac accttcatgg
     8401 tctgcaccgg ccggcttcct gctgacatgg tcgccaaggc ctatcgggcc gggatcccga
     8461 ttgtggtcag caacaatgcc ccattctcat cagggatctc gcttgctgaa cggatgaaca
     8521 tgacccttgt gggatttgcc cggccacccc ggatgagcat ctatacgcat cctgaaagaa
     8581 tccggctgta atggacctgc agcggcattc gtttccccaa acctactttt cctgcaacga
     8641 ttatccgggt cataaagaag gtcggtgtgt tctgaagcga atcctgttgt gaaatcagag
     8701 gattctttcc aatcaaactg cttccggcag ttttatacct ttactcgatg aaaaggccgt
     8761 catcgattgt tcatatgaat gttccaccgc aggtcttggg attacctagt ctgtacacaa
     8821 gtgaaacgat gaaatgataa actctaactc attgtaaaaa tacaacattt accatggatg
     8881 agcagaaaat acagtccata ttccgggaag cgggtatcga gaaagagatc aaatgcccag
     8941 aagcgtttct catatcagaa aaatatggga tctcaaagac cgatatctca cggttctgca
     9001 atgagaacgg gatcaaaatc cgggcatgcc agcttgggtg tttcaaataa tccttgtgca
     9061 tcttcggcat ctgtcttcag gcgaaagata tccattgcgc agtatgggga attttcgggc
     9121 attccctggc atgcccactg ttgtaatgac agatagttct cgcctatccg taaaatgatc
     9181 catgaaacaa gtcggagtcc ctgcacttct atcagaaaag caccccgcag ggaattattc
     9241 atctcctgaa atcgggggga gagtagtgca tgagtcaaac caagaaaatt ctcattgaat
     9301 ggcgccactt ggaattcggg gagattccat gcggccgttg caccgatacc gggaccaatc
     9361 tcctgaaggt gatcacacaa ctgggagatg aggatctcct caacaatgtc gaattggaaa
     9421 ttcagaatac cattctctct ccggataagg tggatgaatc gaatgtgctc ataattaacg
     9481 gcatccagtt ggaacacctt ctcggcacag ggatctcttt ttcgggatgc agctgtagga
     9541 ccgatatcag caaggatcag gtttcatgca aggcggtgca actggaccgg gatgtcttca
     9601 aagcgatacc cgaagagatg atcaggggag ctcttctgga ggttctgaag caggattgat
     9661 cagggaattt cttctgctcc cttggtaaat ctcactccga tcatccgatc tgattatctg
     9721 gcgggtctca attttttcat tggaaggggt ctggtaaaag atagttctaa atatcggcta
     9781 taatcaatct gattcagtga actttatgaa gatcagtgct cgcaatcaga tcccgggaac
     9841 ggtgaaactg ttaaaaaaag gtccggtcac gacagaagta gtgatatctg tcggagctgg
     9901 acaggagatt gtctcgacca taaccaccca ctctgccgaa acgatgaagc tgagggaagg
     9961 atcgaaagta tacgccgtga tgaaggcgtc tgaagtgatg gtggggatcg attaagttct
    10021 tttttcattt cagttcctct tttaaaatcg tgtagcaagg agtgcttcga agtattccaa
    10081 ggggtacgat ccctgttccg gattatacca gacctcgatg cctttctccc cgacacgagc
    10141 gtgaatctcg agaaggccgt atttatggat ggtatgcact ggtcctgcgg cggtatccct
    10201 gatatatgcg tcttgatctc ccaaaaaagc ctggatgtca tacctattga ctgggcgggt
    10261 gagctggtat atacgcagtt cccagtcagg gtacggggag acctgatgtg ctgcaggagt
    10321 actcatgttt cagacttcac cgctgataga acaaatgtac gttcgtagtg ccggcagcac
    10381 gcatgattga ccaaggacat gaatttttca caggtgctgc cggcggaaga caccattgat
    10441 cggctctaaa gctgcatttt catgcaccat ttctggtgca ttgaaataat ttgcctgggg
    10501 cggagataaa catttctatt taataaatgt aaatagttaa ttaattatac tttagtggcc
    10561 ttcttagtcc tatgaaattt tcctggctca ccaccatctc cctggtattg cttgcagcaa
    10621 tcattgtcgc cggttgtact tccgaacagg cacagcctga acaaaaaaac gatctgaccg
    10681 tgtttgctgc cgcttccctc accggtgcaa taactgaaat ggcaaaagag tacgaaacca
    10741 gccattcgga tatccacgtc atcaccaatt tcgacggctc gcaggcgctc aggacgcaga
    10801 tcgaacaggg ggcatatgcc gatgtgttcc tctccgccaa caccaaacat ctgtatgccc
    10861 tccgcgacca gggtctcatg gagaacagca gtatcgggct gtttgcaaag aaccggttgg
    10921 ccgttatcac ccccgcatcc aatcccggga atatcatgac ccttgatgac cttgcccgtc
    10981 ccggcaccag gctcgttatc ggcacgaaag atgtccctgt cggggattat gcccgtcaga
    11041 ttctgaccaa gatgggcaac gataccgcgt atgggccttc ctataactca tcagtccgcg
    11101 caaacgtcat ctctgaagag acgaccgtga cgggagtggt ggcaaaagtc cagctcggcg
    11161 aggccgatgc cggcatcgtg tatgaatcgg atatcacccc tgaaaaccgt aacaagttca
    11221 ccgtaatcgc aatccccgac acgttcaatg tcatcgccga atacccggcc ggtgtcgttg
    11281 cggcatccca gcacaaggaa gaggcagccg ccttcctcgc gtatctcaat ggcgagctgg
    11341 gcagagcaac cttggcgaag tacggatttg cgttgccgtg aactcatgag atcacatgat
    11401 gggaagacga tatttcaaag gaaggaataa ccttcttacc atcggggcag gggtgctcat
    11461 caccctgctc gtcatcttca tcatcacacc gatccttggc ctcttctcgc ggatcacgtt
    11521 cgatcagttc attgcctccc tctctgaccc tgtcgtatgg aatgccctgg ttctgagcct
    11581 ttttactgcg ggtatatcca cgtttgtgat cattctggtt ggcactccgg tggcatggat
    11641 caatgcccgg caccactatc ccgggcgcga gattgtggat accctcatcg accttcccct
    11701 cgtcctcccg ccgaccgttg caggccttgc cctgctcatg gcctttggca ggatgggcct
    11761 gattggaaga tttttttata acttcgggat cagcattgcc ttcacgaccc ttgcggttat
    11821 cattgcacag attttcgtgt cggtgccgtt ctacatcagg caggcacggg ctagcttcga
    11881 acaactcgat gccatgtatg agaatgccgc ccggagtctg ggatcaccac ctgttccaac
    11941 atttttcacg gtcgtcctcc ctctggtaaa agcaggactg atcggggggg ctattatgtc
    12001 ctttgccagg gctctcgggg agttcggggc gaccatcatg tttgccggga atttccaggg
    12061 ccgtacccag acgatgcctc tagccatcta taccaccatg cagggggatt tgaatttctc
    12121 catcaccctc tctgttatcc ttatcatcat ctccttcatc gtgattgctg ttgtaaaatt
    12181 caagctcagg gggtatacgc cacgttagag gtacatcttt ataaaaacct gcgcgacttt
    12241 cccctcacgg ccgaattcag cgtcagggat ggtgagacgc ttgtcctcat cggtgagaat
    12301 ggttcgggaa aatccagcct cctttatctc ctctctggac tccttgctcc tgaatcagga
    12361 tttatatcgc ttgacaaccg cttgctctat gagggaagca aggggacctc gatcccgtca
    12421 gaagagcgcg atatcgggtt tgtcttccag aattatgcca tttttcccca cctcacggtc
    12481 tttgaaaaca tcgcattcgg tctccggcga agaaaagtcc ccgattctga acttcgttca
    12541 agggttgagg aatacctgtc cctcctttct ctcgatacgc tatcaagtat ccggggggac
    12601 cagctttctg gaggcgaacg ccagagagcg gccctggcac gggctcttgt cattgaaccc
    12661 tcactcctct tactcgatga gccggtcagt gctcttgatg cagtggccca ggagaatatg
    12721 cggtcgcaac tccgaaccta tgtaaggaaa tcagatattc cctgtattgc agtaacccat
    12781 tcgttcaggg atgcggtatc tcttggtgac cggcttggcg tcatggagag aggccgcatt
    12841 gtccagaatg gagcactgga agagatcaag ggaagcggaa ataccttcct ccaccatttc
    12901 ttctgtgggt gttcgaagga ttcgtgccgg aggtaaggat gatgcaatgc tcggccagga
    12961 cccctctgtt cctccgaaaa aacggatatt aggtgataag gtgaccggga aaatcccaat
    13021 catcatagaa tggcgccacc ttgacctcgg tcagcccttc agcggccatt gttccgaaac
    13081 cggggcaacc ctgtgggagg tgatcacaac acttggacag gagcactggc ttgatgatat
    13141 tggactggaa ctcaaaaata ccatcctcct tccggaagag actgaggaat cgaacaccgt
    13201 gctcgtaaac gggatccctg ttgagcagat cctcaataca ggtgacacgt tcggttgcag
    13261 cggcgggcag gacacaggga gcgaggtcac accgcttcaa cgggttccac ccgggcggga
    13321 ggtgttgaag gggatcccct atgaggtgct ccgaaaggcc atccttaccg ttctgacaag
    13381 gacctagggg gggtctcaat cattattcac cggatcattt ttcaatctct cgtcatccct
    13441 cattgaacca ttgacctcat ctcacaggac aaaaaggtct attgagttga gatcggaata
    13501 acgatggacc gcccatctat aaaacaaacc tgctctcctg ttgggacata tatataccgg
    13561 atgaaagtgc cggaggcacg ttcctataaa atggaacaga tgatgaaggt gatacgatga
    13621 gaagatgttc tgctgttccc atcgaatcaa tctgataaaa aaaattgaaa aaatggtgag
    13681 ttgcggttac acccagacgt tttcggggaa cgccatgttg gtgtagatat cttcgagccg
    13741 ggccttgtgc ccccgctcca tgtttgcgag ctgggaaaag aggaactgcg tctcggtgtc
    13801 cttcgcaagt ttcgcgagct gggtgtacat ctgca
//
LOCUS       DAOOCO010000030        13763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4079_length_13763_cov_4.517216, whole genome shotgun sequence.
ACCESSION   DAOOCO010000030 DAOOCO010000000
VERSION     DAOOCO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13763
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_13763_cov_4.517216"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK154_03025"
     CDS             <1..673
                     /locus_tag="PK154_03025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW10067.1"
                     /translation="IVMDVTKQEQVQRAIEQTAAEAGRLDLLFNNAGILLMALFERAT
                     LEEWKVMIDTNLWGVIYGVHTAVPIMLRQGSGHIVNVSSVGGIIPYPIQALYNTTKFA
                     VTGLSESLRFEYAEKGLHFSTVFPGPVATPIYEKRLGKADPLMKVPGDAIPADSAATF
                     ILDRVAERKALIIVPEGFHDHLWKSYVSGDPVAEEMLGAHAHNRRLALERGDPAAAAA
                     APMHR"
     gene            825..1331
                     /locus_tag="PK154_03030"
     CDS             825..1331
                     /locus_tag="PK154_03030"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPW10068.1"
                     /translation="MKTIILIGLPGAGKSTIGVILAKTLGMNFIDTDIVMQERTGRLL
                     QELIDDEGVDAFLAIEEDTALSLDADNTVIATGGSVVLSDRAMKHLKSKSIIIYLIIS
                     FEEMVRRLENITTRGIVLFPGQSLSDMYDQRIPLYEKYADIRIGCSDGDFERILEAVV
                     SEIRESMR"
     gene            complement(1484..2803)
                     /locus_tag="PK154_03035"
     CDS             complement(1484..2803)
                     /locus_tag="PK154_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10069.1"
                     /translation="MKNDTTQENPPCQEQLQQYRAKRHEVDLSIEVVLLVVGGLFFLL
                     FGALLFMIDRGMLPYSEGSMYGLFVILVSMQIITMGKTPFGDLLRSWLVVMIGLFTAM
                     LGTLAIFYPGYLAVTIRVLAGLIVLVTGVLGLLQLFTAKDKARVWIKVPGILQQLTIA
                     CILVYGIEMMLGIITLLPGIIPNLLTAILCLIFGVSLLFLAWCIHMVHRQYPLGDGKE
                     APITPPSTNGFCLLKEASLTMGNTFNAYQGVLMILLGFLVLFITLGIFPSFNSDGQIG
                     LLLVLTSLQLLALGQFVGSQVTRSWLVVSFGLLCASAGIFSCIVPGILAGLIQPLLGL
                     QNILTGVLLLGTQIIAPTLYGIRHPPAEPVVLPPIIKRLFLVLAITGIVTILFGINTI
                     APVLLPNLLGMIAYAVLIPLLIITMGLLTLITISVTQRLQQGAETAP"
     gene            complement(2924..3757)
                     /locus_tag="PK154_03040"
     CDS             complement(2924..3757)
                     /locus_tag="PK154_03040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW10070.1"
                     /translation="MEHKAYYQDKICLVTGANSGIGYAMSEELLKRGATVYMAGRDQK
                     KISAAAGQLFPFRDHVRTLVVDVTRQDQVQKAIEGTAAEAGGLDMLFNNAGIFKPMLF
                     EHGTIDDWREMIDTNLWSVIYGVHAAIPLMIGQGSGHIVNTASLAGLVPYPMQVIYDT
                     TKYAVVGLSESLRYEYAEKGIRVSVICPGDIATPIFSKLVDSKGQGAVIPKEAYPVDR
                     AASYILDHVAEGDGRIIVPYEPNHLIYGGYILGDKNVEEFLLKHAHDHRIRFEKEGRF
                     L"
     gene            complement(3786..4580)
                     /locus_tag="PK154_03045"
     CDS             complement(3786..4580)
                     /locus_tag="PK154_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPW10071.1"
                     /translation="MFRIQDNLDYNMPVHFGGVRLVPGTVATQRATALVIGYETAREE
                     LERYIPGEFELRIPEVQVMFSTFSRINWLAGSCYNLINVSSPVRFQGKKDQIEGDYTL
                     VTWENKTEPILRGREQTGIPKIYADIEDLHEMGSCFHTTASYEGSRFLTMHFEATSPM
                     AGGERDRIQAALGSMNTLGWRYIPKINGPGADLSQFILFPQRMEVETVLAGTGSLQWY
                     EMTWLEHPTQNHIINSLAALPIRKVTRAILAKGMVVLDRMASRAIE"
     gene            4768..5496
                     /locus_tag="PK154_03050"
     CDS             4768..5496
                     /locus_tag="PK154_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPW10072.1"
                     /translation="MKTKFGYSPSIERSRPADALNQAVLAEKMGFDSVWVDDHFLPVP
                     HMTECGFAWTWMSSAFEATERVFFATAVTAPIMRYNPAIVAHAFATMGAMYPGRVGIG
                     VGSGEELNETAVLQGEWPTPGKRLDMLDEALKVMSLLWTSTGPTSFSGSYYHLKNAIM
                     PTRPEKPIPVYFSAIGPRASRMAGLLGDHLITIARDPKIVRTSLSQTSRQGRERQAKT
                     RRRWNVPLPSSTFTTRIILSIPKQ"
     gene            5430..5696
                     /locus_tag="PK154_03055"
     CDS             5430..5696
                     /locus_tag="PK154_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10073.1"
                     /translation="MERALAVLYFYDPDHLVDPEAMKVSAAEAKIEVAGEGSQMELWH
                     SPEDIIRRIEEWKRLGFDHLIFGNFSADCDGGLRVFDKVLPHVD"
     gene            complement(5879..6736)
                     /locus_tag="PK154_03060"
     CDS             complement(5879..6736)
                     /locus_tag="PK154_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW10074.1"
                     /translation="MDTTDYYRNKICIVTGGNSGIGYALCEELLKRGATVYMAGRDPA
                     KVEQAAGHLAMHNDRIRTLIVDVVHQQQVQKAIEDTAAEAGRLDMLFNNAGIGHTGPY
                     EKDTLADWNVMIDTDLWSVIYGIHAAVPIMLKQGSGHIVNTSSIGGLIPVPFQSLYNT
                     TKFAVTGLTESLRYEYAGKGIIFSTLCPAFIATAIFNKGLDGSVGTEKIPEGAYPADK
                     AASYCLDRVAERHGIIVVPEEPFTTMWKRCTTGDPSAEKFLSTMAELRRREVEEGKAP
                     QAIPPLSTL"
     gene            complement(6895..7473)
                     /locus_tag="PK154_03065"
     CDS             complement(6895..7473)
                     /locus_tag="PK154_03065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW10075.1"
                     /translation="MDFFEKAYQERPPWDIGRPQQEIVRLEQTGQITGTVLDVGCGTG
                     DNALFLASCGHEVVGLDSVERAIEAARRKADERSLSATFIRGEILSLREIGRTFDTVI
                     DSGLFHTLSDNERLLFVRNLHKVLVPGGSYFMLAFSNLEPAGYGPRRITKQEIHDLFS
                     DGWRVNGIQAAVFESRTRKEGSRAWLSSITRL"
     gene            complement(7810..8118)
                     /locus_tag="PK154_03070"
     CDS             complement(7810..8118)
                     /locus_tag="PK154_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10076.1"
                     /translation="MTKFYMKWKMNSMTVPGDPAERVKYWLALLEGVKAQLKSGQLLD
                     WGITCDSNEGYCFAESDETSLHATVVTWLPYIQFDIKPVIGVDDVIANVKKAAAAAKK
                     "
     gene            complement(8453..9166)
                     /locus_tag="PK154_03075"
     CDS             complement(8453..9166)
                     /locus_tag="PK154_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10077.1"
                     /translation="MTLVVKEVRAKSILSHSKIHRYVVNPYTGCQHGCTYCYARFMKR
                     VTGHKEPWGEFVDVKINAPELLEKEIRRKQQGTVWVSGVCDPYQPLEERYQLTRQCLK
                     ILARNEWPVIIQTRSPLVLRDIDIIREASGFEVGLSITTADEEIRKIFEPEAPPIPER
                     IAALGKLHSEGIRTFAMIAPLLPGAEGLAPLLKGTIDYCIVDRMNYHYADWVYRKYGL
                     EFALTDDFFSRMSQELSSL"
     gene            9449..11338
                     /locus_tag="PK154_03080"
     CDS             9449..11338
                     /locus_tag="PK154_03080"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10078.1"
                     /translation="MKGNPILALLVIVLVGFLITSATGAMVTEVATAGPSLVLSPSSG
                     KAGSWAKAAGSMFSEEGDPEFPPETVIRFNGIEVASAYGGGSFTIPFQVPSGIPPGAY
                     PVQAVRGSQAATATFTVVTLPVANFAMDPASGTGPAPLVVQFTDTSTGSPTSWWWSFG
                     DDSTSTLPNPSHTYRKNGTYSVTLTVTNMAGNNARKRTITVYEPTLMVSPLSGKAGSM
                     VTVIGSSFYYDEKRASRITLFFNGIEITRDIPMTRSGTMGSFTASFIIPADTAPGSYT
                     VRAESQADGAETPFTVTNLAPRALIDATPPSGKAPLSVHFNGSRSYDEDGSIRSYSWN
                     FGDSKSATGTAVDHTFSQPGEYHVILTVTDNQGSSGTSSFIIRTDNTPPIADARGTPP
                     SGQDPLTVNFDGTFSYDPDGKIRSFSWDFGDGSGEQTASTSHQYQDPGSYTAVLTVTD
                     DKGAADQDEVAIMVGNEPPVAMIRLSPESGTVPLKVTFDGSRSSDPDDTSLTYSWDFG
                     DGFRAGSRTTAHTYEEEGTYQVSLVVTDPHGGSDRAVVTVVAESPFPVLPITGGSLII
                     ICTAIALWYIRQQIINRRRTIPEFHITTDSGLEFDAGGMGRQEEFPDITVEVKSGIWK
                     EGDER"
     gene            11374..11883
                     /locus_tag="PK154_03085"
     CDS             11374..11883
                     /locus_tag="PK154_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10079.1"
                     /translation="MIDKAITGLVKSSHREDFTEIITWTIRTGLGEKDAQDQLINTLT
                     TCISKELSKGLVQVSIQQLEGEREIRATYEQSLQPIYPRIDFIVKSGAFDVWTTTYWF
                     RVTSTVKLDDLALRMKKDEITGFQSGIMQAFVTLAYCGHEKENPEPFTLFEKKKVLEV
                     KLPEVVRFE"
     gene            complement(11964..12422)
                     /locus_tag="PK154_03090"
     CDS             complement(11964..12422)
                     /locus_tag="PK154_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10080.1"
                     /translation="MTETPTLPSIDEEPFMVESETVNAIRARAPLEAIILDFMLIDGR
                     AVVVDMTLEEVREGIKARYEIDPFEDAHQPRAAKEAPSIVPYVAGIYSTAEVAKIIAA
                     RLIDAGFPVLVEQLPEAPPDADVIGTPEGGLVQDHQGPVWVISTGYAGRS"
     gene            12755..13642
                     /locus_tag="PK154_03095"
     CDS             12755..13642
                     /locus_tag="PK154_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10081.1"
                     /translation="MDPDKGSFLELVYEARIKDIVKGRDDQHFEASGVSLKDGYLHII
                     FDDVPSLLRIRPDWRYIAEEPALLHLKGTGAGYEDITFQSSTGHWFCLIEAVKTGEDT
                     YLPRIDEFDKSFSFIRSCWLNFPIRTGNKGFEGLSIMHHGGVEYLLCLCEGNDCKGGE
                     KGAKPGKGRIQVFQQGAEQWEHGGTITLPKAVRYRDYVSLALHNGSLTVLSQASSALW
                     TGHIRSDGGEWDDLFEDDGQTYLFPRDEKGRSIYCNLEGVAWLGNGRLVVVSDKAKHD
                     QPGRCTRKDQSIHIFTLPD"
BASE COUNT         3251 a         3665 c         3651 g         3196 t
ORIGIN      
        1 cattgtcatg gatgtgacca aacaggaaca ggtgcagagg gcgatcgagc agactgctgc
       61 agaggctggc cgactggacc tgctcttcaa caatgccggg atccttctca tggcattgtt
      121 cgagagagca acgctcgaag agtggaaagt gatgatcgat accaacctct ggggtgtgat
      181 ctacggcgtg cataccgccg tgccgatcat gctcaggcaa ggatccgggc atatcgtgaa
      241 tgtttcatct gttggcggaa ttattccgta cccgatacag gctctctaca atactacgaa
      301 gttcgcggtt accggtcttt ccgagagcct gcggttcgaa tatgccgaga aagggcttca
      361 cttctccacc gtctttcccg gccctgtggc gaccccgatc tatgaaaaac ggctcgggaa
      421 ggcagatccc ctgatgaagg ttcccggcga tgccattccc gctgactccg ctgcaacctt
      481 catccttgac cgggtggccg aacgaaaagc gctcatcatc gtccctgaag gatttcatga
      541 tcacctctgg aaaagctatg tgagcggcga tcccgtagct gaagagatgc ttggtgccca
      601 tgcccataac cgcagactgg ctctggaacg gggcgatccg gcagccgcgg cagcggcacc
      661 gatgcatcgg taagatatgg aggaggatca tctccccatt cccttccttc cggttctgtc
      721 tcttcatcac ctcatggatt tatccatgag acgaaaacgt aatgattatc ggggtgaaac
      781 tgcgggtaag atagtatcaa tgaccaggga gatccgggaa tcagatgaaa accatcatcc
      841 ttatcggcct gcccggtgcc gggaagagca ccataggcgt tatccttgca aaaactcttg
      901 ggatgaattt tatcgatacc gatatcgtga tgcaggagcg taccggaaga ctcctgcagg
      961 aactcatcga tgatgaaggg gttgatgcat tcctggctat cgaggaggac acggcactct
     1021 ctctcgatgc tgacaacacg gtgattgcca cgggcggcag tgttgttctc agcgatcggg
     1081 caatgaaaca cctgaaatcg aaaagtatca tcatttacct gattatttcc tttgaagaga
     1141 tggtacgaag gctcgagaac atcacgacaa gggggattgt actttttcca ggccagagtc
     1201 tctccgacat gtacgaccag agaatcccgc tctacgagaa gtatgccgat atccggatcg
     1261 gttgttctga tggtgatttc gagagaattc tcgaagcagt cgtcagtgaa atccgggaaa
     1321 gcatgcggtg actgacctcc ctccattcca catacacccg gaaatgaggc aggaatattc
     1381 ctggtgaatg agcccaattc gggaggcggt cccggtgccc tgcgagagat tctggcgatt
     1441 gacaggaaaa gagaggggaa tattcctgat gtatgatcac gattcagggc gcggtttcag
     1501 cgccctgctg gagtctctga gtaactgaaa tggtaatcag ggtcaggagc cccatggtga
     1561 tgatgaggag cgggatgagg actgcatacg cgatcatccc gagcaggttt gggagcagga
     1621 ctggtgcaat ggtgttgata ccaaacagga tggtcacgat ccctgtgatc gcaaggacga
     1681 gaaacagccg ctttatgatc gggggaagca ctaccggttc tgccgggggg tgtcgtattc
     1741 cgtacagggt cggggctatg atctgcgttc caaggagcag gaccccggtg aggatgttct
     1801 ggagcccgag gagcggctgg atcagtccgg caaggatccc cgggacaatg cacgagaaga
     1861 tacctgccga agcacagagc agcccgaagg aaaccaccag ccaggaccgg gtcacctggc
     1921 tccccacgaa ctgaccgagg gcaagcaact ggagtgaagt gagaaccagc agaagcccga
     1981 tctggccatc cgaattgaac gaggggaaga tccccagggt gatgaagagc acaaggaacc
     2041 cgaggaggat cataagcact ccctggtagg cattgaaggt attccccatt gtcagtgagg
     2101 cctcttttaa caggcagaat ccattcgttg agggaggggt tatcggcgct tccttcccgt
     2161 ctcccagggg gtattgtctg tgtaccatat ggatgcacca ggcgaggaac aagagggata
     2221 ccccaaagat cagacacagg atggctgtca gcagattggg gatgatgccg ggaaggagcg
     2281 tgataatccc cagcatcatc tcgatcccat acaccaggat acacgcaatt gtcagctgct
     2341 gcaggatccc gggaaccttt atccacaccc tggccttgtc ttttgcagtg aacagctgca
     2401 ggagcccgag gactcccgtg acaaggacga tcaggcccgc aaggacacgg attgtcacgg
     2461 ccaggtatcc tgggtaaaag atcgcaaggg tcccgagcat tgccgtgaaa aggccaatca
     2521 ttacgaccaa ccaggaacgg agaagatcgc caaacggggt ctttcccatc gtgatgatct
     2581 gcatcgagac gagaatgaca aagagtccgt acatgctccc ctcgctatag ggaagcatcc
     2641 ccctgtcaat catgaacagg agcgcaccga agaggaggaa gaacaatccc ccgaccacca
     2701 gcaggactac ctcaatggag aggtctactt cgtgccgctt tgcccgatat tgctgcagtt
     2761 gctcctgaca gggaggattc tcctgtgtcg tatcgttctt catgcggatt caccaccatt
     2821 gcaaagagca tgtgatcctg acacgaaggg gaggtctggt gtattttgta ctccattatt
     2881 gcccaataca gaacatttca caatctctcc tccttttgat aaatcagagg aaccgtccct
     2941 ctttctcgaa ccggatcctg tgatcatggg catgcttcag caggaactcc tccacatttt
     3001 tatctccaag gatgtatccc ccgtaaatca ggtggttggg ttcatagggc acaatgattc
     3061 gcccatctcc ttccgcaacg tgatcaagga tataggatgc agccctgtcc accggatatg
     3121 cctccttggg gatgacggct ccctggccct tgctgtcaac gagtttggaa aagatcggag
     3181 ttgcgatatc ccctgggcag atcacagaga ccctgatccc tttctctgca tattcatacc
     3241 gcaggctttc tgaaagcccg acgaccgcat acttcgtcgt atcatagatc acctgcatcg
     3301 ggtacgggac gagcccggca agtgaagcgg tgttcacaat gtggccggag ccctggccga
     3361 tcatgagagg gatggcagcg tgaacgccat agattacgct ccacagattg gtatcaatca
     3421 tctctctcca gtcatcgatg gttccgtgct cgaacagcat gggcttgaat atcccggcat
     3481 tgttgaagag catgtccagc ccgcccgcct cggctgcggt cccttcgatg gccttctgta
     3541 cctggtcctg tctggtcaca tcaaccacga gtgttcgaac atgatccctg aacgggaaaa
     3601 gctgcccggc tgctgctgaa atcttcttct ggtcccgccc tgccatgtag acggtcgctc
     3661 ctcgtttgag gagctcttca ctcatggcgt atccgatgcc ggagttcgcc ccggtcacga
     3721 gacagatctt gtcctggtaa tatgccttgt gctccatacc tatcctgtat tccttttcgg
     3781 tgcggttatt caatcgcccg gctcgccatc cgatcgagga caaccatgcc cttggcaagg
     3841 atcgcccgcg tgacctttct tatggggagg gcagcgaggc tgttgatgat atggttttgc
     3901 gtaggatgct ccagccaggt catctcatac cactgcaggc ttcccgttcc cgcaaggacc
     3961 gtctctacct ccatccgctg cgggaaaagg atgaactggg agaggtcggc gccagggcca
     4021 ttgatcttcg gaatatacct ccatcccagg gtattcattg accccagggc agcttgtatc
     4081 ctgtccctct cgccgcctgc catcggactt gtcgcttcga aatgcatggt aaggaaacgg
     4141 gagccttcgt aactggcagt tgtatgaaaa caggagccca tctcatgaag gtcctcgata
     4201 tccgcataga tcttaggaat tccggtctgt tcccttcctc tgaggatcgg ttcagtctta
     4261 ttctcccacg tcacgagggt ataatcccct tcaatctgat ctttcttccc ctgaaacctg
     4321 accggggagg agacgttgat aaggttatag caggagcccg ccagccagtt gatccgggag
     4381 aaggtactga acatcacctg tacttccggt atacggagct cgaattcccc gggaatgtag
     4441 cgttcgagct cctcacgggc tgtctcatat ccgataacaa gggcagttgc ccgttgcgtt
     4501 gcaaccgtgc cgggaaccag cctgactcct ccaaaatgga cgggcatatt gtaatcgaga
     4561 ttgtcctgga tccgaaacat aaaagcctcc gacagtgtgt ccagccgaga gggggataaa
     4621 cgtgtcgatc ctgatttgat acgggacaag acactgcgat tgattaaacg ttatgtcatg
     4681 ttaatccatg gacaatccct gctgatcaag cctgtcccct ggtgaatttt tttatcttaa
     4741 atgaggatga tgtaacaagg atgcgctatg aagacaaagt tcggctacag tcccagcatc
     4801 gagcggtcca gaccggcgga tgccctcaac caggcggtac tggcagagaa gatgggattt
     4861 gactcggtct gggttgatga tcatttcctg cctgtgcccc acatgaccga gtgcgggttt
     4921 gcgtggacct ggatgagctc tgcttttgag gctacagaac gggtattctt cgccacagcg
     4981 gtcactgccc cgataatgcg gtacaaccca gcgattgttg cgcatgcctt tgccaccatg
     5041 ggagcgatgt acccgggacg ggtggggatc ggggtcggtt ctggggaaga gctgaacgag
     5101 acggcggtac tccaaggtga atggccaacg ccgggaaagc ggctcgacat gcttgatgag
     5161 gcacttaaag tcatgtcgct cctctggaca agcaccggac cgaccagctt ttccggcagc
     5221 tattaccacc tcaaaaatgc catcatgccc acgaggcctg agaagccgat ccctgtctac
     5281 ttcagcgcca tcggtccacg ggcatcaagg atggccggtc tcctcgggga ccatctgatc
     5341 acgatagcga gagatccaaa gatcgtcagg acgtcattat cccaaacttc acggcagggg
     5401 cgagagaggc aggcaaagac ccggagacga tggaacgtgc ccttgccgtc ctctactttt
     5461 acgacccgga tcatcttgtc gatcccgaag caatgaaggt ctctgccgcg gaggcaaaaa
     5521 ttgaggtcgc cggagaggga tcgcagatgg agctatggca cagtcccgag gatatcatca
     5581 ggaggatcga ggaatggaag cggctgggat tcgatcacct catattcggg aactttagtg
     5641 ctgactgcga tgggggactt cgagtatttg acaaggttct cccgcacgtg gactgactgg
     5701 gtcatcaact cctttttcca gggattgatt ttctcccttg tttctctgca tcacactttt
     5761 tctccatcac gagtccaacc tgtgatacta tcggcaggga cccggcaggt ccaggaagaa
     5821 aaaggcgaat aggatccggc ctctgtcacc tcttttttca ccatattgtt tctatcattt
     5881 acaaggttga taaaggagga atcgcctggg gggccttccc ctcctcaacc tcccttcggc
     5941 ggagttcggc catggtggac aggaattttt cagctgaagg atctcccgtg gtgcagcgtt
     6001 tccacatcgt tgtaaacggc tcttcgggaa cgacaatgat accgtgccgt tccgcaaccc
     6061 tgtcaaggca gtacgatgcc gccttatctg ctgggtatgc cccttcggga atcttttcgg
     6121 tgccgacgct gccatcgagt cctttgttga aaatggccgt tgcaataaaa gcaggacaga
     6181 gggtggaaaa aatgattcct tttccggcat actcataccg caatgattca gtcagcccgg
     6241 tgaccgcgaa cttcgtcgta ttatagagag actggaacgg gaccgggata aggccgccaa
     6301 tagatgaggt attcacgata tggcccgatc cctgcttcag catgatcggc actgccgcat
     6361 gaataccata aataacactc cagaggtcag tatcgatcat cacattccag tcagcaaggg
     6421 tgtccttctc gtaggggccg gtatggccga ttccggcatt attgaacagc atgtccagcc
     6481 ttccggcctc tgctgccgtg tcttcgattg ccttctggac ctgctgctga tgtaccacgt
     6541 caacgataag tgtgcgtatc cgatcgttat gcatagcaag gtgcccggcg gcctgttcca
     6601 ctttcgctgg gtcgcgtcct gccatgtaga cagttgctcc tcttttgagg agttcttcgc
     6661 acagtgcata tccgataccg gagttccccc cggtaacgat acagatttta ttccggtaat
     6721 aatccgtggt atccatgctg tatcccttcc tgtttcagct gcagaagtgc cggagacccg
     6781 tcacagtccc tttgtgttcc gaatgccgat agttccgctc tgatgcgtgt ttatatatac
     6841 ctaacgatat tcctgctccc gctgcatcat cgttctatgg tgcatatcat agtgtcacag
     6901 gcgtgtaatc gacgatagcc atgctctgga tccttccttt ctcgtgcggc tctcaaagac
     6961 tgcagcctgg atcccgttga ccctccatcc gtccgaaaac aaatcatgta tctcctgctt
     7021 tgtaatccgg cggggaccgt atccggccgg ttccaggttg ctgaacgcaa gcatgaaata
     7081 gctgcctccc ggcaccagga ccttatgcag gttcctgaca aacaggagac gttcattatc
     7141 agagagcgta tggaacaaac cggaatcgat gacagtatcg aatgtcctgc cgatttcacg
     7201 aagagacagg atttctcctc gaatgaatgt tgcagaaagg ctacgttcgt ctgccttcct
     7261 ccgggcagcc tcgatcgccc gctcgaccga atcaagaccg acaacttcat gaccacagct
     7321 cgccaggaag agggcattat ccccggtccc gcatccgaca tcaagcactg tgccggtgat
     7381 ctgcccggtc tgctcgagac ggacgatctc ctgctgcggc cttccgatat cccagggcgg
     7441 cctctcctga taggcctttt caaagaaatc cataaccgtg aggcgatttc ctttcagaaa
     7501 taatgatggt attggatgtg tcccacagtg tcagtgggat acgtgattca acgtgaacta
     7561 tcaagaaaag ggtgtgcctg aggtgcgccc tatcacatca caggagatac cacagcggcg
     7621 gaggtaaacc aatggcgtaa ccccagggta tgtctcttac tcggaagaaa ttcctcgaaa
     7681 ggtgaagaaa acagggttgc agtatccttc taccctcaat aaaaaaatac atacgttcat
     7741 taaggataca aggatgcaag atgaaaggcg gcggtatttt ccctcgaaaa aaaagggagg
     7801 aggcatctct tatttttttg ccgcggctgc agcctttttc acatttgcaa tcacgtcatc
     7861 gactccgatc accggcttga tatcaaactg gatatacgga agccaggtca cgacagttgc
     7921 atgcagggag gtttcatcgc tttcagcaaa acagtaccct tcgttgctgt cacaggtgat
     7981 accccagtcc agcagctggc cggatttcaa ctgggctttt accccttcaa gcagcgccaa
     8041 ccagtatttc acacgttctg ctggatctcc agggacggtc atcgagttca tcttccattt
     8101 catatagaat tttgtcattg catcacacct cttgctggaa tatctgctct gatcgtttct
     8161 gctgcgatta aaaagagggg gaatccaaat ctcccgatca attcctgtgg tacttccaga
     8221 gccgatagcg gatatggtga tggttggaat aaaactatcg catgacaggc atgcagacaa
     8281 tagcaccacc aatataacct attgtgagga tgctgttgtt gagaggggca gaggtgatcg
     8341 ggagaggaga atccaccata ggtctctcat tcaccgccca gtcaggcgga acagacagtc
     8401 ctggagttcc gcaaaatttt attcagcgtt tgttttcgat tgccggtttc tgttacaggg
     8461 aagagagctc ctggctcatc cgggagaaga agtcatccgt aagggcgaac tcaagcccgt
     8521 atttccggta cacccaatca gcgtagtgat agttcatgcg gtcaacaatg caatagtcaa
     8581 tcgttccttt gagaagtggg gcaagcccct cagctccagg gagcagcgga gcgatcatcg
     8641 caaaagtcct gatcccttcg ctgtgtaatt tcccgagtgc ggcgatccgt tccggtattg
     8701 gcggcgcctc tggttcgaag atcttcctta tctcttcatc cgcagtggtt atcgagaggc
     8761 caacctcaaa cccactggcc tccctgataa tgtcgatgtc acgcaggacc aggggagaac
     8821 gggtctggat gatcaccggc cattcgttcc gggccaggat cttcaggcac tgtctggtca
     8881 gctgataccg ttcttccagg ggctggtacg ggtcacacac cccgcttacc catacggtcc
     8941 cctgttgttt ccttcgtatc tccttctcca gtaattccgg tgcattgatc ttcacatcga
     9001 caaactcgcc ccatggttcc ttgtgtcctg tcacccgttt catgaagcgg gcatagcagt
     9061 aggtgcatcc atgctggcag ccggtatagg gattgacaac gtaacgatgt atcttcgagt
     9121 gggagaggat ggactttgcc cggacttcct tcacaaccag ggtcatttca gagcttcgtt
     9181 gttctctatt cttccccagt gatatgatac cttgggagca accggcaccc ttaatatccc
     9241 aaaaaaaata accacccctc aagaggggaa tacttctatg gcctgcattt caaccgggat
     9301 atacccctcc ggacatttcg ttccctcttc ttccacacgt cctggaggtc acaatcgcct
     9361 ctccggaggc ttgtgggagg aaaaagagtc cacaagaaga atctctggag tttgtctcta
     9421 tcgggaaggc agcgacgggt caaaaaaatt gaaaggaaat ccgatcctgg cactccttgt
     9481 gatagtactg gttggttttc tgatcacttc cgcgactggt gccatggtga cagaggtggc
     9541 aaccgctggc ccttcgctgg tcctgtcacc gtcatcagga aaagccggaa gctgggcaaa
     9601 agcggctgga agcatgtttt ctgaagaagg agatccagaa tttcccccag agacggtcat
     9661 caggttcaat gggattgagg ttgccagcgc ctacgggggc ggctctttta ccatcccatt
     9721 ccaggttccc tccggtatcc ctcccggggc ctaccctgtc caggccgtca ggggttctca
     9781 agcggcaact gcaacattta cggtggtgac gctcccggtt gcgaactttg cgatggatcc
     9841 tgcttccggc acaggccctg cacccctggt ggtgcagttt accgacactt caacgggttc
     9901 gccgacatcg tggtggtgga gtttcgggga tgacagcacg tccacgctgc cgaatccgtc
     9961 gcacacgtac cggaaaaacg gcacttactc ggtaacgctg acagtcacca acatggcagg
    10021 aaacaatgca agaaaacgaa caattactgt ctacgagccg accttgatgg tatcccctct
    10081 ttcagggaaa gcgggcagca tggtgacagt gatcggaagt tcattttatt atgacgagaa
    10141 gagagcgtcc cggataaccc tgttcttcaa cgggattgaa atcacccgtg atattcccat
    10201 gacaaggagc gggactatgg gttcttttac ggcatcgttc atcattcccg cggatactgc
    10261 accggggagc tacacggtcc gggctgaaag ccaggcagat ggcgcagaga caccattcac
    10321 ggttaccaac ctcgccccca gggccctcat cgatgccacc cctccatcag gaaaggcacc
    10381 cctctctgtt catttcaacg gttcacgatc ctatgacgag gacggttcca tcagatccta
    10441 cagctggaac ttcggagatt cgaaatcagc gaccggaaca gcagtggatc ataccttcag
    10501 ccagccaggg gagtaccacg tgatcctgac ggttactgac aaccagggct cttcgggaac
    10561 ttcctcgttc atcatacgaa cggacaacac ccccccgatt gctgatgcac gtggtacccc
    10621 tccttcgggc caggatcccc tgacggtcaa ttttgatggt accttttcct atgatccgga
    10681 cgggaagatc aggtcttttt cctgggattt tggcgatggc tctggtgagc agaccgcaag
    10741 cacaagccac cagtaccagg atccgggttc ctatactgca gtcctcacgg taaccgatga
    10801 caagggagcc gccgatcagg acgaggtggc aatcatggtc ggaaacgagc ctcccgttgc
    10861 catgatacgt ctctcaccgg agagtggaac cgtacccttg aaggtaacct ttgacgggtc
    10921 gcgttcctcc gacccggacg atacaagcct cacctattcc tgggatttcg gggatggatt
    10981 cagagcaggt agccggacaa cggcgcacac ctacgaggaa gaggggacct accaggtctc
    11041 acttgtggtg accgatccgc acgggggatc agaccgggca gtggtgaccg tggtagcgga
    11101 atcgccattc cctgtcctgc cgattactgg cgggtcactg attattatct gtactgccat
    11161 cgctctctgg tatatccgcc agcaaataat caaccgcagg cgtaccatac cagagttcca
    11221 tattaccact gacagcggac tggaattcga cgctgggggt atgggaaggc aggaagaatt
    11281 tcctgatatc acggttgagg tcaagtcagg gatctggaaa gaaggtgatg aaagatgaac
    11341 gtttcactga caaacccggt tgtggattcc gaaatgattg ataaggcaat aaccgggctg
    11401 gtaaagagca gccacaggga agacttcacg gagattatta cctggaccat cagaacaggg
    11461 cttggagaga aggatgccca ggatcagctg atcaataccc tgacgacctg tatctcaaaa
    11521 gagctgagca aaggcctcgt tcaggtctcc atccagcaac tcgaaggaga gcgtgaaatc
    11581 agagcgacat atgagcagtc gctgcaaccc atctatccga ggattgattt catcgtgaag
    11641 tcaggagcat tcgacgtatg gaccactacc tactggttcc gggtgacgtc aacagtcaaa
    11701 ctggatgacc tggctctccg aatgaaaaag gatgaaatca ccggtttcca atcgggcatc
    11761 atgcaggcgt tcgtgaccct tgcctattgc ggccatgaga aggagaatcc ggaaccgttc
    11821 acactctttg agaagaagaa ggtactggaa gtgaaactgc cggaagtggt gaggtttgaa
    11881 taaggagatt ttttgagaat tcacggttat ctccaccgta tcccatcaat gcgatggtaa
    11941 aatcccaaaa aacagcgtag gtcctaactc ctcccggcat acccggtgct tatcacccag
    12001 accggaccct ggtgatcctg caccaaccct ccttccggtg tcccaattac atcagcatca
    12061 ggaggagcct cgggaagttg ttccaccagg acggggaatc cggcgtcgat cagcctggcg
    12121 gcgataatct ttgccacttc agcggtactg tagatccctg cgacatatgg gactatcgaa
    12181 ggggcttcct tggctgccct tggttgatgt gcatcctcga agggatcgat ttcataccgt
    12241 gccttgattc cctcccggac ctcttccagg gtcatgtcga ccacgacagc cctcccgtcg
    12301 atcagcataa aatccaggat gatcgcctca agcggtgccc tggccctgat ggcattcact
    12361 gtctccgatt caaccatgaa cggctcctca tcgatggaag ggagggtagg tgtctctgtc
    12421 atcttcttca gcgtcccagt ctcttagggt atccagcata aacctcttct gattcaggct
    12481 tatctgttcc gctcttgtcc ggatggatgg gatagaaact ggccggatcc attgacctga
    12541 tcccttttcc tcatacgatt ccagccaacc cccaatcgct ggtgaaggaa tcatattacg
    12601 ccatttcctg ctactttctt tcctgataat gaaaaaatga caagtgggtg gaaaaaacac
    12661 ttgtcgtcct cacccgggtt cagaacaagg aggggcccct gcaacctata aggtaatata
    12721 cctcccgtga cagacatccg ggcaggggtg aacgatggat ccggataaag ggtcattcct
    12781 tgagctcgtg tatgaagcca ggatcaaaga cattgtcaaa gggagggatg atcagcactt
    12841 tgaagccagt ggagtatctc tgaaggatgg ataccttcac atcatatttg atgatgtccc
    12901 atccctttta cgcatccggc cggattggag gtacatagct gaggagcctg cactgctcca
    12961 tctgaagggc accggcgccg gttatgaaga tatcacattc cagtcatcaa cgggccactg
    13021 gttctgcctt atcgaggccg tcaaaacagg tgaggatacc tatctgcccc gtatcgatga
    13081 atttgacaaa tcattctcct ttatcagaag ttgctggctg aacttcccga tcaggacagg
    13141 caataaagga tttgaaggct tatcgatcat gcaccatggg ggggttgaat atctcctgtg
    13201 cctctgtgag ggaaatgatt gtaagggcgg tgagaagggt gcaaaaccgg gaaaaggaag
    13261 aatacaggtc tttcaacagg gtgcggaaca atgggagcat ggcggcacga tcacgctgcc
    13321 gaaggcggtt cggtacaggg actatgtcag tctcgctctc cataatggtt ctttaaccgt
    13381 actctcgcag gcttcatcgg cgctgtggac aggacatatc cgttcagatg gtggggagtg
    13441 ggatgatctc ttcgaagatg atggccagac ctatcttttc cctcgcgatg agaaagggag
    13501 gagtatctac tgtaacctgg aaggggtcgc ctggcttggc aacggccggc tggtggtggt
    13561 atcggacaaa gcaaagcatg atcagcccgg gagatgtacc cggaaagacc agtccatcca
    13621 catcttcacc cttccggatt gagagattct ctcataggaa atgcctccgg cattttcatc
    13681 ttttatgctt atggcattca gaggatccat gttatttttc acgttcccct ggccatggca
    13741 tacatctgtt ccggcaggat ggc
//
LOCUS       DAOOCO010000031        13612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4145_length_13612_cov_5.642768, whole genome shotgun sequence.
ACCESSION   DAOOCO010000031 DAOOCO010000000
VERSION     DAOOCO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_13612_cov_5.642768"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..>1303
                     /locus_tag="PK154_03100"
     CDS             103..>1303
                     /locus_tag="PK154_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10082.1"
                     /translation="MTSDFTVLDPNGLTLSVNYPYKSQWNPSLVYGSGQYFVTWNREV
                     SSGEYNLNGQILQEIALNAPPVANANGPYTGTEGGAGVVFSAAGSSDPEGAPLTYRWD
                     FNNDGVYDITTTQETQTWDWAGNEYHGDVKLEVSDGTLTAMDTARVDINNVAPTTNIL
                     PIPTYTLTAGADIANTGKIFDGDFTTKWMAASGDPNAQWIKIDLGSVRSIAGFRETSG
                     VYAGKPYTATVSTDDVMYTSMTSGTLTQWAFGEHLFTTSQDARYIKINIAQNDGYGVL
                     AEFRVIETGILTSAIEDESIAFTGIFTDPGIDQWTSTWAFGDGQSASANVISGTHSLT
                     GSHAYTTAGTYTVTLRVGDADGGVGTDQRTIVITPKDTTPPGITVPPSPVTPDPDGSV
                     SVQVGDAV"
     assembly_gap    1304..1403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1404..2767
                     /locus_tag="PK154_03105"
     CDS             <1404..2767
                     /locus_tag="PK154_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HPW10083.1"
                     /translation="VVTATDAAGNTATDTFTVVIDKTAPIVTVPEVPVTPDPDGSVSV
                     QVGDAVSATYTINDASAVTVTPDNGVITIPGFTDDGTYTVVVTATDAAGNTATDTFTV
                     TISQNHAPVLTSPGDKTVESGSTLTFMLFASDEDGDTLTFSAPTLPSGASLEAATGRF
                     SWTPASTQIGNHQVQFTVSDHDLSDSESITITVTDVAPTDNEAPVIQNMVANPNPVPI
                     NTPITLTATVDDSGTGNSGITSVQYSTDSTTWEAMSATDGIFDEPIEEVTATIAGFDT
                     ASVHTIAVTVTDSEGNIAQSEEILLAVYDPSAGFVTGGGWIMSPEGAYTPDPAMTGKA
                     TFGFVSKYKKGATLPTGETEFQFKAGNLNFMSTSYEWLVVAGAKAMYKGTGTINGAGE
                     YGFMLTAIDGAIKGGGGMDKFRIKIWEKATDTPVYDNQLNAPDTDDPTTVISGGSIVI
                     HTK"
     gene            2819..2941
                     /locus_tag="PK154_03110"
     CDS             2819..2941
                     /locus_tag="PK154_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10084.1"
                     /translation="MMNITIEPFYYTTPLQSITKMLAGEQRKAPLFVGREQVEG"
     gene            3299..3469
                     /locus_tag="PK154_03115"
     CDS             3299..3469
                     /locus_tag="PK154_03115"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPW10085.1"
                     /translation="MGIYGSFARGTNTAGSDVDIWILVSKHSLEIEAHTAELRHTLSH
                     SLGYDVHMLILT"
     gene            complement(3727..4440)
                     /locus_tag="PK154_03120"
     CDS             complement(3727..4440)
                     /locus_tag="PK154_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10086.1"
                     /translation="MIIGTDLTRYYGLGAVQVRALDGVSLKIQRGEFVGVMGPSGSGK
                     STLLHMLGLLDRPTSGSLLIDGVDVGALDEQARTVFRLRRLGYVFQDYALVPELTAKE
                     NVYLPSMARGMTPQECTLVCSDILGVVGLGDRIHHLPSQLSGGEQQRVAIARALVNRP
                     ALLLADEPCANLDTRNSKVILDLFRKLNHDLHQTIVMVSHEEWHKQYFDRIISMKDGV
                     IETDLPGTREGDSRGNPSG"
     gene            complement(4442..5644)
                     /locus_tag="PK154_03125"
     CDS             complement(4442..5644)
                     /locus_tag="PK154_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW10087.1"
                     /translation="MFEDAKVAFFLAKRSIVRGNKGTLSLTILIIGMVFVNLIFLPSI
                     ISGVAVLFNQQTIDYSYGNLVIEPKENQVYINSADELQKKIERIPGVTGVSPRYITGA
                     TIVYKGNEVTPLLYAINPDDDRQVLKIASKVASGEYLGDGDTDQILIGYRLAGNYDER
                     LDVIPSLGGVVVGKVVEVTFNNGVTREYRVKGIINSDSYDVDSNAFITRDEYESVYGL
                     EDKANQMLVRTLETGNENEMRTTLMSFGVQEKIWTWQEKSANFLNQIIGSFNIINFIG
                     TVVSLVIAIVVIFIVIFINTVYRRKQIGILKAIGIDRSIIIRSYLFQALFIFALGTLI
                     GGGFLVLILQLLAANPIVFPGGPVAPVVDAMLILRSTLSLLVVSLIAGYVPAWITTRE
                     DILSAIRG"
     gene            complement(5637..6881)
                     /locus_tag="PK154_03130"
     CDS             complement(5637..6881)
                     /locus_tag="PK154_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW10088.1"
                     /translation="MRLPQKATRLFIAVLGILLLCSIVSAATSVPSVIITEYEVSPAV
                     LLPGEQGIISVTVRNTADRATLTQSYLSDTTETTTNIDINAPIDSVTMVGNGIQVLSE
                     GYYRVGDIGPGQSIPLSFLFKAPAQAGIYFPEIWIRVSDARSLKYPIPVNVNSEVAIQ
                     KRPQISIEKQLPASVTPGDDFNATILFKNTGQLSASNLKISVNSSTPSITAKTASTYH
                     IDRLGPDESQAIAMAFSTDRNAPLGLRPVTLVMEFQEPDGSIQQQVETLGVNVRGRAE
                     LGISQITTDPARIQKGEQFTLIIRIENTGTDDAKSVDAMIDIPLPGTKEAFVGTIEPA
                     NDAPAPFNLQADRAGEIPYTLTIRYEDDYGPGEQVQNLRLNVYGSNNTFLILGGVLLL
                     VLAGIGGYWYFKLRRKPGTGNV"
     gene            7200..7418
                     /locus_tag="PK154_03135"
     CDS             7200..7418
                     /locus_tag="PK154_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPW10089.1"
                     /translation="MEFKIPIEQDEDGWYIVTVPSLSGCVSQGKTEEEAKKNSAEAIE
                     LHLSALARDGIPLYHRPGVKETFVAVDL"
     gene            7484..7645
                     /locus_tag="PK154_03140"
     CDS             7484..7645
                     /locus_tag="PK154_03140"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPW10090.1"
                     /translation="MNDQKGSHNHLMHPQRRPLTVPNHPEIARGISSLRTQISPLKNF
                     WSCDNSRLC"
     gene            complement(8102..8821)
                     /locus_tag="PK154_03145"
     CDS             complement(8102..8821)
                     /locus_tag="PK154_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10091.1"
                     /translation="MNRTTIIIIAVLACVVLGVGAFVALSGDGGQVQITPGDATIDAL
                     VSIQKNPPPDYIEELTGIIEISEDQQVRDAAINVLTDIAIRRSETDEIMDFLKDLAAH
                     EEDPVIMSAAYAGIDLIRDTYPLPPMGSLNLSVRGDVKKGGEVEIIATITSTTDIPKA
                     VLGLDFPPDSVEMLTPPVYYTALSANQPATHTFPLRFLQTGEVKIPVQLTLSTDLTDY
                     EQIDRNVLFVVREGNGEYSIL"
     gene            complement(8835..11603)
                     /locus_tag="PK154_03150"
     CDS             complement(8835..11603)
                     /locus_tag="PK154_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10092.1"
                     /translation="MREKVSITVLILLICFLCTVLVVHAQTQCPSPCTCMLPSDAAKM
                     KGYSLCGGKQIICGYDKAQNPLYCYQPPAATTTPKTPTPTPTTTTTKTLSPTSTPAMV
                     ITASPGSCPSICSCLLSADAKKMGYTLCGGKETLCGYDQYKNPQYCFQKPATTAPVVT
                     TTPATPVPAACPSTCSCLLPADGKKLGYPLCGGKETLCGYDQYKNPQYCFQKPATTVP
                     SSIPATPLTQAIQSVEELPIATLLTPSPTGTFLPVQDVAAGRAGLPGCLLSGRISGFP
                     FDPETLKVRIQEIRIVEGMAPSAGLTHWEPSSMEPIGDPIIVPVVPVETSTREFVDYT
                     YGARVNCTATYLVRPEYYPYAPGEHCEWQGTWAPSARTVIMEGADVGGNDFAFSPRSV
                     RAPGIEFGADPAEPRINDPVTVTIRIRDEEDIRSFSYKIDYTLEDRSSQAGSWVNFIP
                     NEDAEGEYVRVRIPDAMRAASAVVTAKACDAGGNRNEQSFVLNFGTCTDGIRSGEEEG
                     VDCGGPCTACGMVAIRGRILYEEMSSDLSVSLGDFPARRIKFLLLDDDGDLTGLKTTN
                     NDGTFSVVIPDSNRGRSLRIRIGDCSNFRAGFNYATKIAYDVDGCNIYVKWTSDRFTV
                     PENGDLDLGDLTIKKDTDGDFEFSIDHRGYDGTYPCYTLLPCEEETDNRDGGSVYFSI
                     ADAILSGREYANHYRGGTDSIGKPTVQYPDRRWSMYYPPTHIIDIPAASADWPDQGFN
                     DGSVIHEYGHHLSYTISYLNPWGGDHTSCTAKNGYFAWNEGWAEYFGTIVVFRDQVFG
                     SPPSSLSGPDFGFSSIENFWCNGTPLNADGSTVEGHVAAVLWDLADDPATFPDSVNES
                     WDTVSGHEDLTFRVFDNEFGGATGHAYIPTLCNFVNYGWNDYDELRGEDDGDEIDPLL
                     TYMGVLNC"
     gene            complement(11986..12258)
                     /locus_tag="PK154_03155"
     CDS             complement(11986..12258)
                     /locus_tag="PK154_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW10093.1"
                     /translation="MKYRLEYTARARKDLHKLDRTTAQRIILALHGLSDSPYSQIKKL
                     KGTNPGHPVYTFRIGNQYRAILSIHDTVLVIQVLEIEDRKQVYRDF"
     gene            complement(12255..12491)
                     /locus_tag="PK154_03160"
     CDS             complement(12255..12491)
                     /locus_tag="PK154_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HPW10094.1"
                     /translation="MQAASTIYIREDLKEKLQALKQHPRESYNDVIERLVTLAVDQDP
                     LNDEAIKGLEEALEDIRHRRLHSEAEIMAEFGVE"
     gene            13379..13561
                     /locus_tag="PK154_03165"
     CDS             13379..13561
                     /locus_tag="PK154_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10095.1"
                     /translation="MADKKRGCPYLSRRKQGKWTECACSLPAGGACPDPRCMARPDAE
                     THPDADHREVVPDDGE"
BASE COUNT         3158 a         3713 c         3644 g         2997 t
ORIGIN      
        1 gcggagtata gtggtggatg cggacccaat tccctgcaga tagagtatga tagcaacaac
       61 tggttggttg tctgggctgg atgtcaactg aatgggactc gtgtgacctc agattttact
      121 gtcttggacc ccaatgggtt aacgctttca gtaaattacc cctataaatc ccaatggaat
      181 ccaagtcttg tatatggcag tggacagtat ttcgtcacgt ggaacaggga ggtttcatct
      241 ggggaatata atctcaatgg tcagatactg caagaaattg cattaaacgc accaccagta
      301 gccaatgcga acggacccta caccgggacg gaaggcggag cgggagttgt cttcagtgca
      361 gcgggttcga gcgatccaga aggagcaccc ctgacatacc ggtgggactt taacaacgac
      421 ggtgtgtatg acatcacgac aacccaggaa acccagacct gggactgggc cgggaatgag
      481 tatcacggcg atgtgaagct ggaagtgagc gacggcaccc tgacagcaat ggataccgcc
      541 cgggtggata tcaacaacgt ggcacctacc acgaatatcc tgccgatacc gacatatacg
      601 ctcacagccg gcgcagacat cgcaaacacc ggtaagatct tcgatggcga ctttaccacc
      661 aaatggatgg cggcaagtgg tgatccgaac gcccagtgga ttaagattga tttgggcagt
      721 gtccggagca tcgccggatt ccgtgagacg tccggtgtat acgccggcaa accgtataca
      781 gctacagtat ccacggatga cgtcatgtat acctccatga ccagcggtac cctgacgcaa
      841 tgggcgtttg gggaacacct gtttacaacc tcgcaggatg cccggtatat caagatcaat
      901 atcgcacaga acgatggtta tggagtactg gccgagtttc gggtgatcga gaccgggatc
      961 ctgacttctg ccatagagga cgagtcgatt gcttttacgg ggatcttcac ggacccgggc
     1021 atcgaccaat ggacatctac ctgggccttc ggagacggcc agagtgcaag tgccaatgtc
     1081 atctccggga cccactcgct gaccgggtcg catgcctata ctacagccgg cacgtatacc
     1141 gtgaccctcc gggtagggga tgcagatggc ggtgtaggga cagaccagcg aaccatcgtg
     1201 ataaccccta aagacacgac acctccaggg attacggtac caccatctcc ggtcacccct
     1261 gacccggacg gctcggtgtc agttcaggtc ggagacgcgg tctnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnttgtcgt cacagcgacg gatgcagcag ggaacactgc
     1441 tacagacacg ttcacggtag taattgacaa gaccgccccg atagtcaccg ttccagaagt
     1501 gccggtcacc cctgacccgg acggctcggt gtcagttcag gtcggagacg cggtctctgc
     1561 gacatatacc atcaacgatg catccgcagt cacggttact ccggataatg gcgtgattac
     1621 tatccccggg ttcactgatg acggaaccta tactgttgtc gtcacagcga cggatgcagc
     1681 agggaacact gctacagaca cgttcacggt gactatctcc cagaaccacg ccccggtcct
     1741 cacctcccct ggtgataaga ccgtggagtc cggatccacc ctgaccttca tgctatttgc
     1801 ttcggatgaa gatggggaca ccctcacgtt ttcagctcca acgctcccat ccggagcatc
     1861 gttagaagcg gccacaggcc ggttcagctg gacgcccgcc tcgacccaga ttgggaacca
     1921 ccaggtacaa ttcactgtca gcgatcacga cctcagcgac tctgaatcca tcaccatcac
     1981 tgttactgat gtcgccccca ctgataacga ggccccggtc atccagaaca tggtcgctaa
     2041 tccaaacccg gtcccaatca acaccccaat aacccttaca gcaaccgtcg atgattcagg
     2101 gaccggaaac tcgggcatca catccgtcca gtattcgacc gacagtacga cctgggaggc
     2161 gatgagtgca acggatggca tcttcgatga gccgattgag gaagtgacag caacgatcgc
     2221 tggctttgac accgccagtg tacataccat tgcagtaaca gtaaccgatt ctgagggcaa
     2281 tattgcccaa tctgaagaga tcctcctcgc cgtgtacgac ccctctgccg gcttcgtcac
     2341 cggcggcggc tggatcatgt caccggaagg agcgtacact cctgaccctg ccatgaccgg
     2401 caaggcaacc ttcggattcg tctcaaagta caagaaaggg gcaacactac cgaccgggga
     2461 gaccgagttc cagttcaagg cagggaacct gaacttcatg tccaccagct atgagtggct
     2521 cgtcgttgcc ggtgcaaaag caatgtacaa gggtacgggg acgatcaacg gtgccggtga
     2581 atacgggttc atgctcactg cgatcgatgg ggcgatcaaa ggcggaggcg gcatggacaa
     2641 attccggatc aagatctggg agaaggcgac cgatacaccc gtatatgata accagctgaa
     2701 tgctcctgac acggacgacc cgacaacggt tatcagcggc ggatcgattg tgatccacac
     2761 caaataatta ccccccccat ttttcaaacg agaaatcctt cttcagtagt cttcatcgat
     2821 gatgaacatc acaatcgaac cattctatta cacgacgccg ttgcaatcaa taacaaaaat
     2881 gcttgccggg gagcagcgaa aggcaccgct gtttgtcggg agggaacagg tggaggggtg
     2941 atgaaaccta ttgtttgttt ctcaatcaga aacaaattgt ttcccgaaca gaaacaatga
     3001 gataacatgg ggggactggg tatgattcca gaactattca aatcagaaga ccgcttgcga
     3061 atcctgcggt atgtcgcaga gagggagaac gtcactgtgc agaccgttgc aaaagccacc
     3121 agggtctcaa aacccgttgt gtcacggtat atgcacctgc ttatcaagaa cgggctgtgt
     3181 aaacgcactg gcaggattct gtcgtgggtg ccatctccag tgggatccgc aatgaaacga
     3241 tttctgaacg tagtacttca ttgacgaaca tcttcctccc accacatggg tagagagcat
     3301 tggaatctat ggatcctttg cacgggggac aaatacagct ggcagtgatg ttgatatatg
     3361 gatcctggtg tccaaacatt ctctcgagat tgaagcacac acagcagagc tccgccatac
     3421 gctctcccat tcccttgggt atgatgtcca catgctcatc ctcacatgaa agaaattaca
     3481 ggatctctct cgacaggatc ccccatttta tgaggagttc atgaaagatc aggttgtcat
     3541 ccgaggtgcg gcgatttgac aaaacttgaa gactgtttcg aagaggggaa tttacacccc
     3601 attccagcat ctgcaatcca tgaaggcttt ttctgaggag agggggaaga gtttccagta
     3661 gtacctgatt ccaacagggg agtacttgca cctcctgtca tttttcacct ggaaaaaatg
     3721 tcgtgattat ccggagggat tgcctctgga atccccctcc ctcgtgcccg ggagatccgt
     3781 ttcgatgacc ccgtctttca tggagatgat ccggtcgaaa tactgtttgt gccattcctc
     3841 gtgggagacc atgacgatgg tctggtggag gtcgtggttc agcttccgga agagatcgag
     3901 gatcaccttc gagttccggg tatcgagatt ggcacagggt tcgtcggcga ggaggagggc
     3961 cggccggttc acgagcgccc gggcgatcgc cacccgctgc tgctcgccgc cggatagctg
     4021 ggaggggagg tggtggatcc ggtcgcccag tcccacgacc ccgaggatat cgctgcagac
     4081 cagcgtgcac tcctgggggg tcattccccg ggccatcgag gggagataca cgttctcctt
     4141 ggcggtcagc tcggggacca gggcatagtc ctggaagacg tacccgagcc gtcgcaggcg
     4201 gaagaccgtc cgggcctgtt catcgagggc cccgacatca accccgtcga tcaggaggga
     4261 tccgctggtg ggacggtcaa gcaggccgag catatggagc agggtcgact tcccgctccc
     4321 gctcggtccc atcaccccga caaactcccc tctctgtatc ttcagggaca ccccgtcgag
     4381 cgcccggacc tggaccgccc caaggccgta gtatcgtgtg agatcggttc cgatgatcat
     4441 gtcaacccct gattgcagac aggatatcct ctcgggtcgt gatccaggca gggacgtacc
     4501 cggcgatgag cgagaccacc aggagactca gtgtggaccg gaggatcagc atcgcatcca
     4561 ccaccggggc gaccggcccc ccgggaaaga cgatcgggtt tgccgcaagg agctgcagga
     4621 tcagcaccag gaacccgccc ccgatgaggg tcccgagggc aaagatgaac agcgcctgga
     4681 acaggtacga ccggatgatg atcgagcggt cgatcccgat cgctttcagg atcccgatct
     4741 gcttccgccg gtagaccgtg ttgatgaaga tcacgatgaa gatgaccacg atcgcgatca
     4801 ccaggctcac caccgtcccg atgaagttga tgatgttgaa actgccgatg atctggttca
     4861 ggaaattggc agacttctcc tgccaggtcc agatcttctc ctgtacccca aatgacatca
     4921 gggttgtcct catctcgttc tcgttcccgg tctctagggt ccgcaccagc atctggtttg
     4981 ccttgtcttc cagcccatac accgactcat actcgtccct ggtgatgaac gcgttggagt
     5041 cgacatcata ggagtcggag ttgatgatcc ccttgacccg gtactcccgg gtcaccccgt
     5101 tattgaacgt cacctccacc accttcccca ccaccacacc gccaagggag gggatcacat
     5161 caagccgctc atcatagttc ccggcaagcc ggtacccgat caggatctgg tcggtgtcgc
     5221 catcccccag gtactccccg ctggcgacct tggaggcgat cttcaggacc tgccggtcat
     5281 catccggatt gatcgcatac aggagcgggg tcacctcgtt tcccttgtac acgatcgttg
     5341 cccccgtaat ataccgggga gagaccccgg tcacgccagg gatccgttca atcttcttct
     5401 ggagctcatc ggcactattg atatacacct ggttctcttt cggctcgatc accaggttcc
     5461 cgtaactgta gtcgattgtc tgctggttaa agagcactgc taccccggag atgatggagg
     5521 gaaggaagat caggttgaca aacaccatcc cgatgatcag gatggtgagg gagagggtcc
     5581 ccttgtttcc ccggacaatc gaccgcttcg cgaggaagaa ggcgaccttg gcatcctcaa
     5641 acattgccgg ttcccggttt ccgacggagt ttgaagtacc agtaaccgcc aatccctgcc
     5701 aggacgagga ggagcactcc tccgaggatc aggaacgtat tgttgctccc atacacattc
     5761 aggcgaaggt tctgcacctg ttcgccagga ccatagtcat cctcgtaccg aatggtcagc
     5821 gtgtagggga tctccccggc ccggtcggcc tggaggttga aaggtgccgg tgcatcattg
     5881 gccggttcga tggtgccgac gaatgcctcc ttggtgcccg gaagcgggat atcgatcatg
     5941 gcatccaccg acttggcatc atcggtgccg gtattctcga tcctgatgat cagcgtgaac
     6001 tgctctccct tctggatccg ggcaggatcc gtcgtaatct gggagatccc gagctccgct
     6061 ctccccctga cattcacccc gagcgtctcc acctgctgct ggattgaccc gtccggttcc
     6121 tggaattcca tcaccagggt gaccggccgg agccctaggg gggcattccg gtcggtcgaa
     6181 aacgccatgg cgatcgcctg gctctcatct ggtcccagcc ggtcgatatg ataggtgctg
     6241 gcggtctttg cggtgatgga gggggtagag gagttcaccg agattttgag attgcttgcc
     6301 gagagctgcc cggtattctt aaagaggatg gtcgcattga agtcatcgcc gggagtgaca
     6361 ctggcaggca gctgcttctc gatactgatc tggggccgct tctggatcgc cacctcgctg
     6421 ttcacattga ccgggatcgg atacttcagg ctccgggcat ccgagacccg gatccagatc
     6481 tcgggaaagt agatcccggc ctgtgccggg gccttgaaga gaaacgagag ggggatcgac
     6541 tgccccggac cgatatcccc aacccggtaa tacccctcgc tcagcacctg gatcccgttt
     6601 cccaccatcg tgacgctgtc gatcggggca ttgatatcaa tattcgtggt ggtctcggtc
     6661 gtatcagaga gataggactg ggtgagggta gcccggtcag cggtattcct gaccgtcacg
     6721 ctgatgatcc cctgctcccc ggggagcagg accgcaggag agacctcgta ctcagtgatg
     6781 atcacgctcg gaacactggt ggctgccgac acgatcgagc agagcaggag gataccaagg
     6841 actgcgatga aaagtctggt ggccttttgc ggcaatctca tgaagtattc tgtgccagag
     6901 ctggtcaata atactatggg aacgaccaaa aagcccggcc aatcgacggt tgacctcatg
     6961 agtacctcag cacttgtgat accctatttc cccttgagga attccggaag cggatacctg
     7021 gctgtggaat ccttcactgc gatacaggaa cgagggaaaa agcgaagata ttgcatgctc
     7081 cagggaactg ggattccagt ttactatcag ggagttcgac gaccggaatt tattaactga
     7141 ttaccaatga tgttggaaca acagatacca tagacccata taagattcag ggagcaggta
     7201 tggaattcaa aattccgatt gaacaggatg aggatggctg gtatatcgtt accgtgccgt
     7261 cactctcggg ttgcgtctcc caggggaaaa cagaggagga ggcgaagaaa aatagtgccg
     7321 aagctatcga acttcacctc agcgccctcg cacgggacgg tattcccctt tatcatcgcc
     7381 cgggggtaaa agaaacgttt gttgccgtcg acctatgacg gatacactcc ccatcctttc
     7441 cggaaaggat gttataaaag ccctcggtaa agtaggctat acgatgaatg accagaaagg
     7501 aagtcataac cacctcatgc atccgcaaag acgcccactg actgttccca accacccgga
     7561 aattgcccgc ggaatatcat cattaaggac gcagatctca ccattgaaaa atttctggag
     7621 ttgtgataat tcgagattgt gctgatcaac aggcgtctcc tgctcaccgg ctaccgatag
     7681 aaccgtgcat acacaaggtc atgatcgatg accacctcct ctgcatcaac cctgcagaac
     7741 ggacagttca cgtgttccat gacagatccc tttttggttt tcatgaaagg ggaggtgagg
     7801 aagatcgaaa aaaaatacct caatgcatat gtcgatgcct tcaccctatc atccgaacgt
     7861 gtcgaaatac atcagtttca tcgacacatt ctacggttcc atgagcagct tcagcaatac
     7921 tgtgtgaaca gagatcttct cccttccgac ctttcgcgag gcaaggatcc cggtctcttc
     7981 ccgcgttttc ggttgtaaag atgcagtctg ccgttgtgca atacacgctt ctaccacgaa
     8041 ctctcactcg ccagagtgcc cgattctcag aaacagtgat cgattcaggt gcaggaaaaa
     8101 attagagaat tgaatactcg ccattcccct cccttaccac gaagagaacg ttccggtcaa
     8161 tctgttcata atcggtcaga tcggtgctga gggtcagctg gaccgggatc ttcacttcac
     8221 cggtctggag gaaccggagc gggaaggtat gggttgctgg ctgattggct gacagggcgg
     8281 tgtaatagac gggcggagtg agcatctcga ccgaatcggg agggaaatcc aggccgagaa
     8341 cagcctttgg tatgtcggtg gtagacgtga tggtcgcaat aatctcaacc tcacctccct
     8401 tcttcacgtc accacgaacc gaaaggttga gtgatcccat cggaggcagg ggataggtat
     8461 ccctgatgag atcgatgccg gcataggccg ctgacatgat gaccggatct tcctcatggg
     8521 cagcgaggtc cttgaggaaa tccataattt catcggtttc cgatcgccgg atggcaatat
     8581 cggtcaggac attgattgct gcatcccgga cctgctggtc ctcgctgatc tcgatgatcc
     8641 cggtcagttc ttcgatataa tcaggcggtg gatttttctg gatcgaaaca agggcatcaa
     8701 tcgttgcatc tccgggggtg atttgaacct gtccaccatc ccctgaaagg gcaacaaagg
     8761 ctccgacgcc caggacaacg caggcaagaa ccgcgataat gatgatagtt gttctgttca
     8821 tagcaggttc accttcagca gttcaggacc cccatgtagg taaggagcgg gtcaatctca
     8881 tccccatcat cctcgccacg gagttcgtca tagtcattcc agccatagtt cacgaaatta
     8941 cagagcgtag ggatatatgc atgcccggtc gctccaccga attcattgtc aaagacccgg
     9001 aaggtcagat cctcgtgccc gctcaccgtg tcccaggact cattgacaga atccgggaag
     9061 gtagcgggat catcggcaag atcccagagc acggcggcga catgcccctc gaccgtagaa
     9121 ccgtcagcat taagcggtgt cccattgcac cagaaatttt cgatactgct gaatccaaaa
     9181 tcgggtccac tcagggaact tggcggggat ccaaagacct ggtcacggaa cacgacaatg
     9241 gtgccgaagt actcagccca gccttcattc caggcaaagt acccgttttt cgcggtgcaa
     9301 ctcgtatggt ctcctcccca gggattcagg taactgatcg tataggaaag atgatggcca
     9361 tactcgtgaa tgacgcttcc gtcattgaat ccctggtccg gccagtcagc actggctgcc
     9421 gggatatcga tgatatgggt tgggggatag tacatgctcc agcggcggtc aggatactgg
     9481 accgttggct tgccgataga atcggtcccg ccccggtagt ggttggcata ctcacggccg
     9541 gaaaggatgg cgtctgcaat actgaagtac accgatccgc catcccggtt gtcggtctcc
     9601 tcctcgcacg gcaggagggt atagcagggg taggtgccat catacccccg gtggtcgatg
     9661 gagaactcaa aatcaccatc cgtgtctttc ttaatggtca ggtctccaag gtcaagatcc
     9721 ccgttctccg ggacggtgaa acggtcgctg gtccacttga cgtagatatt acagccatca
     9781 acatcatagg caatcttggt ggcataattg aacccggccc ggaaattgct gcagtctcca
     9841 atccgaatcc ggagtgatct tccccggttg ctgtccggta tcactacgga aaacgtacca
     9901 tcgttgttgg tcgttttcag accggtgaga tcaccgtcat catcaaggag caggaacttg
     9961 atcctccggg cagggaagtc cccgagtgag acgctcagat ccgaactcat ctcctcgtag
    10021 aggattcgtc cccggattgc caccatgcca catgctgtac aagggccgcc gcaatccacc
    10081 ccctcctctt ccccgctcct gatgccatcc gtgcaggtgc cgaaattcag gacaaaggac
    10141 tgttcattcc ggttccctcc ggcatcacag gcctttgcag taaccactgc ggaagcagct
    10201 ctcatggcat ccggtatcct gaccctgaca tattcgcctt ctgcatcttc attcgggatg
    10261 aaattgaccc agctgcccgc ctgggaactg cgatcctcca gcgtgtaatc gatcttataa
    10321 ctgaatgatc ggatatcttc ttcgtcgcgg atacggatgg tgaccgtcac cggatcattg
    10381 atcctcggtt ctgcaggatc cgcaccgaac tcaatccccg gcgcccgcac ggaacgcgga
    10441 gagaaggcaa aatcattccc cccaacatcc gcaccttcca tgatcaccgt ccgggcagaa
    10501 ggagcccagg ttccctgcca ctcgcagtgt tcacccgggg cgtacgggta atattcaggc
    10561 cggacaaggt aggtcgccgt gcagttcacc cgggccccat aggtgtaatc cacgaactcc
    10621 cgggtggaag tctcgaccgg gactaccggt acaatgatgg gatcaccgat cggttccatg
    10681 cttgacggct cccagtgtgt cagccccgca gagggagcca ttccctcaac gatccggatc
    10741 tcctggatcc tgaccttcag ggtctccgga tcaaaaggaa aaccggaaat ccttcccgaa
    10801 agaaggcatc cgggcagtcc cgcccttcct gccgcaacat cctgtaccgg caggaacgta
    10861 cccgttggtg agggggtcag gagcgtcgct atggggagtt cttcgaccga ctggattgcc
    10921 tgggtaagag gggttgccgg gatggatgat ggcacggtcg ttgctggttt ctggaagcag
    10981 tactggggat tcttgtactg gtcatacccg caaagcgtct ccttgccccc gcagagtgga
    11041 tatcccagtt tcttgccatc ggcaggaagc aggcaactgc aggtggaggg gcaggcggcc
    11101 gggacagggg tcgcaggagt ggttgtcacc acgggggcag tcgttgccgg tttctggaag
    11161 cagtactggg gattcttgta ctggtcatat ccacaaaggg tctccttccc cccgcagagc
    11221 gtgtatccca ttttcttggc atcagcagaa agcaggcaac tgcagatgga ggggcaggaa
    11281 cctggagaag cagtgatcac catggccgga gtcgatgttg gcgaaagtgt ctttgtagtc
    11341 gttgttgttg gggttggcgt aggtgtcttt ggggtcgttg tcgctgccgg cggctggtag
    11401 cagtacaatg gattctgggc tttgtcatag ccgcagatga tctgttttcc tccacacagg
    11461 ctatatccct tcattttcgc tgcatcagaa ggcagcatgc aggtgcaggg agatgggcac
    11521 tgggtctggg catggaccac cagcaccgtg cacaagaaac agatgagcaa aattaagacg
    11581 gtaatggata ccttctctct caatgataac cccccttggg gtacttggaa agtaccggtc
    11641 ttgtgtatta aaattcaccg gaatgtacgc ggaacataca tagtaaaata agtatgggca
    11701 tgttatgctt ttttacgcgg aacagaacag aaaggaatgc ttcatgatgg agaggagcct
    11761 atgaggagag ataaaagctt gccatgggaa aaacattcat ggtgaaggat gatcacgtct
    11821 ccctgaaatt tttaaaaatt gatgaggttc atgattcgtg agcattgcgt tcacgatttg
    11881 tgaataaatt gttcactatt cttgaacgta ttgatgacag ggagtgaaaa gcagacctcc
    11941 cttcccagac ctcattgagt atcactcttt cagagagccg tccccttaga aatcgcgata
    12001 tacctgtttc ctgtcttcga tctcaagtac ctgaatgaca agaacggtgt catggattga
    12061 caggatggcc cggtactggt tcccgatgcg gaacgtgtat accggatgtc cggggttcgt
    12121 tcctttcaat tttttgatct gtgagtaagg ggagtccgaa agcccgtgca gagccaggat
    12181 gattcgttgg gcagtcgtcc ggtcgagttt atgcaggtct tttcgtgccc gggcggtgta
    12241 ttccagccgg tatttcattc aacaccgaac tcggccatga tctctgcttc agagtggagc
    12301 cggcggtgcc tgatatcctc aagagcttct tcaagccctt tgatcgcttc atcgttcagg
    12361 ggatcctggt ccaccgcaag ggtcaccagc cgctcgatga cgtcattgta ggattccctg
    12421 ggatgttgtt tcaatgcctg gagtttctct ttcagatctt ccctgatgta gattgtggat
    12481 gcggcttgca tagtatcacc tataggtatc tattgatatc aatatgtcga tcgatgatat
    12541 cacccttccg ttgcatacga aaggccgggg agatcaacct cgcctatcac aacccgaaac
    12601 ggacccctag cacatccctg tttcacttga tgctgtcaaa gatcctgcct ccaatataag
    12661 ggccgtctgc caatcctgct tcgagttctt gcttttttat cgaagttgcc tcacacatta
    12721 cgtcaataat gacaacggta caaagttggc tgccaccgct tccgtgatga tgattaaatg
    12781 atggatagtt attaccagct ctatcttcgc atctttagat cctgccgacc tctgacaccc
    12841 cttctcccat gcattttctg cgcgacggaa gatcccgttc atctccccgg tgaaccttac
    12901 tcactccgat acataaaaac atcccttgac agcctccatt caggattccg gcatatctcg
    12961 tcggttcttt ataaatttcc tttctgataa tcgggaatga ggatcaacga accgggggat
    13021 gattactttc acctcgtcaa tcaaaaaatt taaaaagcga cctttgcctc ctcttctttg
    13081 tatgtgaccg gaaaaactct attcatcgtc cgttcggtca catacaggaa atgtggatga
    13141 aaccgaacat gatcacccca ccacgaagtc gtgtggacct gccgatccag agctcccacg
    13201 acaattacaa agaaccgtcc ttttttgata aagaccatga gaacagggag gctgcaccat
    13261 gtgctgcctg aggggttccc ccccggatgt tcatgcccgc aaaagccgga acagagaagg
    13321 aacccgtacg ttcctcttcc tgggaggcaa aaaactttct cctctggagg gtcccctgat
    13381 ggccgacaaa aagagggggt gcccgtacct ttcccggcgg aagcagggga agtggaccga
    13441 atgtgcctgc tcgcttcctg ccggtggcgc atgccctgac ccgcggtgca tggcacgtcc
    13501 tgacgccgag acacatcctg acgctgacca tcgggaggtg gtgccggatg acggcgaata
    13561 ggagggtggg aggaccgccc cgcctcagtt cccggaagca ctacaaccac ta
//
LOCUS       DAOOCO010000032        13454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4226_length_13454_cov_5.506083, whole genome shotgun sequence.
ACCESSION   DAOOCO010000032 DAOOCO010000000
VERSION     DAOOCO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_13454_cov_5.506083"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="PK154_03170"
     CDS             <1..1834
                     /locus_tag="PK154_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPW10096.1"
                     /translation="LKWHGDVPKGDRAWKDNEPPHILMITPESLEVLLMERELSLGLA
                     HLRYLIIDELHAFVESERGVQMRVLIDRLDRISGRKIQRIGLSATVGNPEEILSWLSG
                     SRHGEVLVRIPAPPQPKRFTFVVEEKESARASALARIVRGKKALVFVNSRSDAEAVNT
                     ALAGRVDQLLVHHSSLSPEMRRETERAFSESESSCIICTSTLELGIDIGNLDVVVCIG
                     PPPSVSSFLQRMGRSGRRGKPPYVACILKDACELLCMVAVIESASRKEVEPLHPHKKP
                     YNVLVQQVLLEIVRKRRTSQSHIRRFVRGLFAFREIKPREIDALLGVLDDFGILVGDG
                     DMLMPGPGAESSFGRSNWKDLFSVIKGGSEFRAVTPDGEMIGTLDARFVAGKNRKSFT
                     LGGKSWTFVKSDDSHELVVVVPGEGEKNEIFWTGGRTGFSPVVCQAVGRILSTGGSML
                     PLPEPERALISGVIDALPELIPRGICILEKPGKRNYDVTILTFRGRMFNGILASLIRS
                     ESDRRLTVSYHDFSVTIKNAGKVGVSSTIYDLLMRLQERRTDSGAKGLRTPGTETWKF
                     ASALSPEILREMAFADYYRYPEFLQDFGTVEIFLTDPGGSVPAV"
     gene            complement(1838..2674)
                     /locus_tag="PK154_03175"
     CDS             complement(1838..2674)
                     /locus_tag="PK154_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10097.1"
                     /translation="MHLGFLTIFWRDMLRFYRFKTLLISSLVQPALWMAFFGIAMSSN
                     FNRFTSELPPLPGVPTVDYLTFMAAGVIAMTTLFTSLFGGMSLLFDKNWGLMREIFAS
                     PLPRNHIIFGIALSGMTKSFIQAIIIMVFGILIGAQFFFGYTVIETVLAIVGILLFVA
                     VFSLGFLFLSAAIAISLESPEGMQGIMTLLTMPIFFASNALYPIDAFPPALQAVSVIN
                     PLTYLVTGIRYFAIGDDFQAIGIHYQFTPFQIVFAFLFLVGFAAVMFAIAWWRFRKAV
                     VT"
     gene            complement(2678..3664)
                     /locus_tag="PK154_03180"
     CDS             complement(2678..3664)
                     /locus_tag="PK154_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10098.1"
                     /translation="MTEPIISVKELFHQYGDFTAVDGISFEVSEGEIFSFLGPNGAGK
                     STAINVLITLLPLQQGSATVAGFDVVKDPEQVRESIGIVFQDVTLDRDMTCREILEFH
                     GNLYKMPNAERKDRIEELLALVQLENKQDTRTKNLSGGMKRRLEIARGLMTRPKVLFL
                     DEPTIGLDPQTRLRMWEYIKAVNREGTTIFLTTHYMDEADHLSDRISIIDKGQIIATG
                     TPSELKNTLGEDLIYLETSDGDGTRKILSGMEMVKHITQKPTGIVAMLNGDGTKILPM
                     IIEALSREGIRVSTVNLKKPSMDDVFVHYTGRELRDEGAEKPSMAVTRAGRR"
     gene            3883..4212
                     /locus_tag="PK154_03185"
     CDS             3883..4212
                     /locus_tag="PK154_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10099.1"
                     /translation="MNGDTGRIEHFIAMLDDEDPSKRWRAAETLGRIRDDRAVEPLIL
                     ALDDEDWRVRQKAAWALGKVGDPRALVPLRRALMNEREGVKEIIEEALDEIKAMGYNP
                     VRSADDR"
     gene            4383..5912
                     /gene="lysS"
                     /locus_tag="PK154_03190"
     CDS             4383..5912
                     /gene="lysS"
                     /locus_tag="PK154_03190"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW10100.1"
                     /translation="MDFRDTGLEFDQNRIEELDQLRSEGYSLYPPVFERKERITEIRE
                     KFSAVGHERSEERVVTAGRLYTVRNHGKTIFADLGDEGARIQLYIRKNDIGEAHFDFF
                     TRFIERGDIIGIGGYPFRTKLGELTIWVDDIVLLTKSLNSLPEKFHGLTNVEKRYRQR
                     YLDLIMNIEVRETFRIRSLLVSEIRSYLTAREFLEFETPILQPVYGGANARPFTTYHH
                     CLCQNLFMRIAPELYLKRLVIGGFEKVFEIARNFRNEDIDTRHNPEFSMVEIYEAYRD
                     YNDMMALTEGIISHLVHTICANYRIEFGGEPLDFTPPWRKMSMEDAVKELAGLDTRAY
                     SLEDLKKIARDKRIEGYEAATSWRELLVLFFEEMVEGQLIQPTFIYDFPIENSPLAKK
                     HRSKEGFTERFELFICGMEIANGFSELNDALDQLARFQKQDDMRKAGDLEAQMLDYDF
                     INALGYGMPPTGGVGLGIDRLVMLVTNQSSIKEVILFPQMRSEGDATLKSSGNNEAGI
                     P"
     gene            complement(6096..8333)
                     /gene="hypF"
                     /locus_tag="PK154_03195"
     CDS             complement(6096..8333)
                     /gene="hypF"
                     /locus_tag="PK154_03195"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPW10101.1"
                     /translation="MRRNGRILVRGVVQGVGFRPFVYARAREYGISGYVKNLGSEVEI
                     YAKGDAFEDFLLSIRTGPPLARIDYLSVTPWEGPYPKDFLILPSSPGTMSGMIPPDIA
                     TCEECLADIDSRRGRYRSYWATSCVNCGPRYSIINKLPYDRERTVMQQFPMCEECGDE
                     YADPSCRRHHAQTIACERCGPHLILLDRDGNTVDCRDLIQETARLLDSGALIAIRGIG
                     GFHIACIEESAGELKRRLGRSEQPFAIMVRNSDYVSQIACISSEERLFLDSATRPIVV
                     LEKRDRTAHTGISNLHTIGCMVPYTGLHHLLFSHLTHPLLVMTSANMPGYPMITDIDR
                     AMASLSREVDFFLTHDRSIINRCDDSVVRDGFIIRLSRGMAPRRTRLPLGERTILAVG
                     PELNSNITIYKDDIATTSPHIGNVRNPGTFEYLKETISTMGSLLGVETYDLITHDAHP
                     QFLSTRYARELAEEGGGKLVPVQHHRAHVAAATPDPCIGITIDGVGYGDDGTIWGGEI
                     FAGQIPDYERIGHLEVVPMPGGDLATTYPERMLYGILPESDVQEILLSRGWNDLELDI
                     LKAQLDKSLNVAMTSSTGRVLDAVSALLGICDEKTYDGEPAMKLESAAVGGRASEWDL
                     SFFRNASTEVLSTRSLMKSALREYKSSRSGDHHHISDIAASFQYNLARGIAGLAVHAA
                     HDWDIPFIVLSGGVAYNRMIRETIIDQVREAGLELFINAEYPLGDGCISFGQCRYAAA
                     LDTSA"
     gene            8391..8903
                     /locus_tag="PK154_03200"
     CDS             8391..8903
                     /locus_tag="PK154_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW10102.1"
                     /translation="MSGHIRIVNDPVELIPLLITFNNSCYKQVYDLLNKSWMTEEELS
                     THLKEEGVSHCISILKKGNLIEEQWRMPEPGKRPVKEFRATYNKFRANFQCNLTDLSD
                     LLYIAVSNDEQLRIVVEKVEEELSGGNNSINDIARKFGVSPTFIRGLAKRIPHLDVKG
                     QGLVLLERSQ"
     gene            8887..9696
                     /locus_tag="PK154_03205"
     CDS             8887..9696
                     /locus_tag="PK154_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW10103.1"
                     /translation="MNEASEDPLSSILRSKREVTRFQILVEIAEHQPAVRQQEIADKL
                     GVTPQAVSEYIRELVDDGMVAAHGRGRYDVSRSGIEWVLNHAEALESYARHIRRDIIQ
                     QVSTWTAIAAHDLKEGEEVGVYMKGGWLYAASEPQSATGIVLIDARQGDDVGVIRLSG
                     IIDHTEGVIHVCKVPRVQRGGSQNVKKEQLLAVTSQAEMVAAVGLEAYVALEKAGRIP
                     DMFFGSREGVIEAAFHGIDCAIFIVDEEFTDFLKRLEGVGLSYLIHDLVTP"
     gene            9693..11744
                     /locus_tag="PK154_03210"
     CDS             9693..11744
                     /locus_tag="PK154_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW10104.1"
                     /translation="MRVIVHPQKGNYKILFLDNHSIRGTAFAEISETPKGVRPVQYRL
                     KWGSRQQYRNTPTKDLIASLRKSEVFLTRRDEIFGRFLADFQISSSLVNLCRICLLEE
                     RITPLSDKTGIRYRGSESICPECAKREIRRELAHMGRIGRQSMSHMEEMLLKYRDVDR
                     VLASIQPEQITMSQTLFDRLDAHPVQKTAHLEELPLPRVFVDASGVSSLMPAQQMAVD
                     AGLLYGKDLLVVSATASGKTFIGEMAGMKNLIEGRGQFLFLVPLVALAVQKYQRFNER
                     YGSFTDVGIMIGKSRLNLPENRQAGDRNTGAPVIVGTYEGIDHLIRMGSRLRQIGTVV
                     IDEVQMLEDPDRGHRLDGLVARLKYLAPKAQFLYLSATIGMPKILAKKLGATLVRYDE
                     RPVSLERYLLFLEHKQKIPTEKKLCEEEFRTKSSKGFFGQTILFTNSRARCHVIADAM
                     GPGYAAYHAGLTAQERRDVEAKFLGGKLKAVITTAALGAGVDFPASQVIFDSLAMGIS
                     WLTVQEFNQMAGRAGRPDFHDLGKVVILAEPGGTYGRGSSFTEEEVAMRLLKGEMEEV
                     APEHDLEQSSEEYVANAVVCRGDEQDLEYINNQMIGTLEPVLPLLLEKGMVRQTNGRI
                     ELMPLARVMAEHFIGVERLLEILTLSNKFKDPIEILTELECSALEERKKEKGKGKREQ
                     R"
     gene            complement(11784..12650)
                     /locus_tag="PK154_03215"
     CDS             complement(11784..12650)
                     /locus_tag="PK154_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10105.1"
                     /translation="MPKNTGPEEMQDIAEILGLVEVQLIEPSFALLPDTTFTALLEES
                     EGTQARLLVDQDSDPLCMAVCREDGTWIAGCFHLRPPTGALIARFEYLGGDIFQEERG
                     IWEAAVRGYYSRELALHTTPALEDLNPGRVSLVDSLVGEILGDGAGRSCLDCGCGSGV
                     GSLVLRKRGYKIFSCDNDPSLISLGFARGRLLQEETMLIDASLLHHYVGHCDTGLGLM
                     FGEIHDFNEGMWEQITGELIMLTDRSLITTGTEQEAMRIRNWADQYGKESEIIENTRD
                     PIYDRWICSISS"
     gene            complement(12628..>13454)
                     /locus_tag="PK154_03220"
     CDS             complement(12628..>13454)
                     /locus_tag="PK154_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314615.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HPW10106.1"
                     /translation="GVEVLFRSGAGHRAALAFRGAGLGACVSSNDPKKEGIQPPAFLP
                     LRDSAADRKTTEALNEFIRQSLPILAKHTLNQDRRKRGLPLANMILVRGAGQMGTYEP
                     FSERYGLSGSVISAATLITGIGKSLGLRYVPVEGATGSSDTNLEGKVKAALTELKEQD
                     FVLLNIKGADEAGHDGHAIEKKNFIEHIDAALEPLLYTDECLIVICADHSTPCSIRDH
                     SADPVPLVIWGDDVRTDAVMQFDEVACAQGGLNRITGHSLMPIICDLINRAKKYGA"
BASE COUNT         3165 a         3496 c         3779 g         3014 t
ORIGIN      
        1 cctgaaatgg catggggatg ttccgaaggg tgaccgggca tggaaggata atgaaccccc
       61 gcatatcctg atgatcacac ctgaatccct tgaagtactc ctcatggagc gggagctctc
      121 tctcgggctc gctcacctcc gctacctcat cattgacgag ctccatgcat tcgttgagtc
      181 ggaacggggt gtccagatgc gggtgctgat cgatcgcctg gaccggattt cagggagaaa
      241 gatccagcgg atcggccttt ctgccacggt aggaaatccc gaagagatcc tttcctggct
      301 gtccgggagc aggcatgggg aggtgctggt ccgtatccct gcaccccctc aaccgaagag
      361 atttaccttc gtagttgagg agaaggaatc cgcaagggcc agtgctctcg cccggattgt
      421 cagagggaag aaagcgctgg tatttgtaaa cagcaggagt gatgccgagg ccgtgaacac
      481 cgctctcgcc ggcagggtcg atcagctgct ggtccatcat tcatccctgt ctccggagat
      541 gcggcgtgag accgagaggg cgttctccga atctgaaagt tcctgtatca tctgcacgag
      601 cactcttgag ctgggtatcg atatcgggaa tctcgacgtg gtggtctgca tcggcccccc
      661 ccccagtgtc tcctcgttcc tccagaggat gggaaggagc ggaagaagag ggaagcctcc
      721 ctatgtcgcc tgcatcctga aggatgcctg cgaactcctc tgcatggttg ccgtaatcga
      781 atccgcgagc agaaaggaag tggagccgct acatcctcat aaaaaaccgt ataatgtgct
      841 cgtccagcag gtcctgctcg agatagtcag gaaacggaga acctcacagt cccatatcag
      901 gagatttgtc aggggtctct ttgccttcag ggagataaaa ccccgggaga tcgatgccct
      961 gcttggtgtt cttgacgact tcggcatcct ggtcggtgat ggggacatgc tgatgcccgg
     1021 gccgggggca gagtcgtcat tcgggagatc caactggaag gacctcttct cagtcataaa
     1081 aggagggagt gagtttcgtg cggtgacccc tgatggcgag atgataggga cacttgacgc
     1141 acgtttcgtc gcaggaaaaa acaggaagag tttcaccctt gggggaaaga gctggacctt
     1201 cgtgaagagc gacgactcgc acgagctggt ggtggttgtt cctggagagg gggagaagaa
     1261 cgaaatattc tggaccggtg gaaggacggg gttctctcct gttgtctgcc aggcagtcgg
     1321 ccggatcctc tcaaccgggg gatcgatgct ccccctgccc gaacctgaaa gagcacttat
     1381 ttccggtgtc atcgatgctt tgcctgagct tatcccccgg ggaatttgca ttctggagaa
     1441 acctggaaag aggaactatg atgtcaccat ccttaccttc aggggcagga tgttcaacgg
     1501 gatactcgcc agcctgatcc ggtccgaatc tgatagaaga cttacggtga gttaccatga
     1561 tttttccgta acgatcaaaa atgccgggaa ggtgggtgtg tcttccacca tctacgatct
     1621 gctcatgagg ctgcaggaga ggagaaccga ttccggtgca aaagggctca gaacccctgg
     1681 cacggagacc tggaaatttg cctcggctct ctccccagag attctccgtg aaatggcctt
     1741 tgccgattat taccgctacc ccgaattttt gcaggatttc gggactgtgg aaatttttct
     1801 cacggatcct ggtggatcgg ttccggcggt ttaactatca ggtgaccacc gcttttctga
     1861 acctccacca tgcaatagcg aacataactg ccgcgaatcc caccaggaaa aggaaggcaa
     1921 aaacaatctg gaatggtgtg aactggtagt ggattcctat cgcctggaaa tcatccccga
     1981 tcgcaaagta acggatccct gtcaccaggt acgtgagcgg attaatcacg gatactgcct
     2041 gcagtgcagg gggaaatgca tctatcgggt atagtgcatt gcttgcaaag aatatgggca
     2101 tcgtgagcag ggtcatgatc ccctgcatcc cttcagggct ctcgagcgag atcgcaatgg
     2161 cagcggagag gaagaggaat ccgagggaga agacagcaac aaagaggagg attccgacga
     2221 tggccaatac tgtttcaatt acggtatacc caaaaaagaa ctgggccccg atcaggatgc
     2281 caaacaccat gatgatgatc gcctgaatga aggatttggt catcccggag agggctatcc
     2341 cgaagatgat atggttcctc gggagcgggc ttgcaaagat ttcacgcatt agtccccagt
     2401 tcttgtcaaa gaggagactc atcccgccaa agaggctggt gaagagcgtg gtcatcgcga
     2461 tcacccccgc agccatgaag gtgagatagt caaccgtggg cactccgggc aggggaggta
     2521 gttctgaggt gaagcggttg aaattggaag acatggctat tccgaaaaaa gccatccata
     2581 atgcaggctg gaccagtgag gagatgagca aggtcttgaa ccggtaaaac cggagcatgt
     2641 cccgccagaa tatcgtgaga aatccaagat gcatcggcta cctcctgcct gcacgggtta
     2701 ccgccatgga gggcttttct gcaccttcgt cgcgaagctc ccggccggta taatggacaa
     2761 acacatcgtc cattgagggt tttttcaggt tgaccgtgct aaccctgatc ccttcccgcg
     2821 acaaagcttc gatgatcatc ggaagtatct ttgtgccatc gccgttcagc atggccacga
     2881 tcccagtcgg tttctgcgtg atatgtttca ccatttccat gcctgaaagg atttttctgg
     2941 tcccgtcacc atcgctggtc tcgaggtaga tcaggtcctc accgagagta tttttcagtt
     3001 cggatggtgt tccggttgca atgatctgtc ctttgtcgat gatactgatc cgatcgctca
     3061 ggtgatcggc ttcatccatg tagtgggtgg tgagaaatat cgtcgtgcct tcccgattca
     3121 ctgccttgat gtactcccac atcctcagcc gggtctgggg gtcgagaccg atggtcggct
     3181 cgtcgaggaa gagcaccttt gggcgtgtca tcagtccgcg ggcaatctcg agtctgcgct
     3241 tcatcccgcc ggagaggttt ttggtacggg tgtcctgctt gttctcgagc tggacgagcg
     3301 ccaggagttc ttcgatccga tccttccgct cggcgttcgg catcttgtac aggttgccgt
     3361 ggaattcaag gatctcgcgg caggtcatgt cacggtcaag ggtgacatcc tggaagacaa
     3421 tgccgatgga ttccctgacc tgctctggat cctttaccac atcaaagccg gcaacggtgg
     3481 ctgatccctg ctgtagggga aggagcgtga tcaggacgtt gattgctgtg ctcttgccgg
     3541 ccccgtttgg tccgagaaac gagaatatct ccccttcgga tacctcaaaa ctaattccgt
     3601 caactgcggt aaaatccccg tattggtgga agagctcttt caccgagatt attggttcag
     3661 tcatgcgacc cccatggatc catcatttgt tgtcaaatca gtctgctcat tttagtggtt
     3721 tggacagaga tcccttaata aggattggaa agggaaatgt ctcatgccaa tcacggttgt
     3781 gatgatggag gcatgggcga ttttccatgc tctggatgcc ggataacatt gtatcgattg
     3841 agtggcgatg aaatatctgg tgagaggagt gattggacag ggatgaatgg ggacaccggg
     3901 cggattgagc attttatcgc catgcttgat gatgaagatc cttcgaagcg ctggagagca
     3961 gctgagaccc ttggaagaat ccgggacgac agagcggtcg agccgctcat cctcgcactt
     4021 gatgacgagg actggagagt gagacagaaa gcagcctggg cactggggaa ggttggggat
     4081 ccaagagccc ttgtgccgtt gagaagagcg ttgatgaacg agagggaagg agtaaaagag
     4141 attatcgagg aagcacttga cgagattaag gcaatggggt ataatcccgt gcgctctgct
     4201 gatgataggt aaggttttat gcaagccccg gtcaggatat gactgccgtg gtatatggcc
     4261 cccttctttt ataaaccatt aatacatctg gcggggaaca tttgagggac aatcaatcac
     4321 ttctcccgtc ctggtaggga gatgtgcgtt tcctggacac ggaccaggca gggagtatgg
     4381 gcatggattt cagagatacg ggtctcgagt ttgaccagaa ccggatcgag gaactcgatc
     4441 agctgcgcag tgaaggctac tctctctacc ccccggtgtt tgagagaaaa gagaggatca
     4501 cggaaatcag ggagaagttt tcagcagtgg gtcacgaaag gagcgaggag cgtgttgtca
     4561 ccgcaggacg cctttataca gtccgaaacc acggcaagac gatctttgct gaccttggtg
     4621 acgagggggc acgaatccag ctctacatca ggaagaatga tattggggag gctcactttg
     4681 atttttttac ccggttcatt gagcggggcg atatcatcgg tatcggcggt tatcccttca
     4741 ggactaagct tggtgaactg actatctggg tcgatgatat tgttctcctg accaagtcac
     4801 tgaactccct ccctgaaaaa ttccatggcc tgaccaacgt ggaaaaacga tatcgccagc
     4861 ggtatcttga cctcatcatg aatattgagg tccgggagac cttccggata aggagcctcc
     4921 tggtctctga gatccgttct tatcttactg cacgtgaatt cctcgagttc gaaacaccga
     4981 tccttcagcc cgtctacggc ggggccaatg cccggccctt cactacctac caccactgcc
     5041 tctgccagaa cctgttcatg cgtatcgccc cggaactgta tctgaagcga ctcgtcatag
     5101 gaggatttga gaaggtcttc gagattgcga ggaacttccg taacgaggat atcgatacca
     5161 ggcataatcc agagttctcg atggtcgaga tatacgaagc ataccgggac tacaacgata
     5221 tgatggctct caccgagggg atcatctcac atcttgtcca caccatctgt gcgaactacc
     5281 ggattgaatt cgggggagaa ccgctcgatt tcacccctcc ctggcggaaa atgtctatgg
     5341 aagatgcggt taaggagctt gcgggactgg atacgcgggc gtacagtctt gaggatctga
     5401 agaagattgc cagggacaaa aggattgagg gatatgaggc agcgactagc tggagggaac
     5461 tgttggttct gttcttcgaa gagatggtgg aagggcagct catccagccc acgttcattt
     5521 atgacttccc gatcgagaac tccccccttg cgaagaagca ccgttcaaag gagggattca
     5581 ctgaacggtt cgagctgttc atctgcggga tggagattgc gaacggcttc tcggaactga
     5641 atgacgctct cgatcagctc gcccggttcc agaagcagga tgacatgagg aaagcaggcg
     5701 acctggaagc ccagatgctt gattatgact tcatcaacgc gcttggctac gggatgcctc
     5761 ctacgggagg agtggggctt gggatcgacc gcctcgtaat gctggtgaca aaccagagtt
     5821 ccatcaagga ggtcatcctc tttccacaga tgagatctga aggggatgct accctcaaat
     5881 cctccggtaa taatgaggcc ggaatcccat gattggagtg tcggaagtag tgtatcaggt
     5941 ttgaatgttc cccttttccc tgatctgcat caagaataaa aaaaatccag tcagatcatt
     6001 gacaaggcat cctcccttgc cctcaaaggg gcaagaggat aagcctcgca ggtttttcaa
     6061 taaaaaatga gcgattgcct gggatcagag cacttttaag ctgacgtatc caatgcagcc
     6121 gcatacctgc actgcccaaa tgagatgcac ccgtctccca gcgggtattc tgcattgata
     6181 aacagttcaa gacccgcctc gcgtacctga tcaataattg tctcccgtat catcctgtta
     6241 taggcaacac ccccggaaag tacaataaaa gggatatccc agtcatgagc tgcatggacc
     6301 gcaagcccgg cgatccctcg cgcaagatta tactggaacg aagcggcaat atcggaaatg
     6361 tgatggtggt caccagaccg gctggactta tattccctga gtgcgctttt catcaagctc
     6421 cttgtggaca gcacctcagt ggaagcgttg cggaaaaacg acagatccca ctcactggct
     6481 cttccaccaa cggctgccga ttccagtttc atcgcaggtt caccatcata ggttttttca
     6541 tcgcatattc caaggagtgc agagaccgcg tcaaggaccc tccctgtact gctcgtcatg
     6601 gcaacattca ggcttttatc cagttgtgct ttgaggatgt ccagttcgag atcgttccag
     6661 ccgcgtgaca ggaggatctc ttggacatcg gattcaggca ggatcccata gagcatcctc
     6721 tccgggtagg ttgtcgccag atcgccgcct ggcataggaa caacctccag atggccaatc
     6781 cgttcatagt cagggatctg acctgcaaag atctcccctc cccagatcgt tccgtcatcg
     6841 ccatatccaa ctccatcaat ggtaatccca atgcagggat caggggtggc agccgcaaca
     6901 tgggcccggt ggtgctggac cggaacgagc tttcctcccc cttcctcagc cagctcacgg
     6961 gcataccggg tggaaagaaa ctggggatgg gcgtcgtggg tgatcaggtc atacgtttct
     7021 acaccaagga ggctgcccat ggtgctgatg gtctccttca ggtattcaaa agtcccggga
     7081 ttcctgacgt ttccaatgtg gggggaagtg gttgcaatat catccttgta gatggtgata
     7141 ttggaattca gttcaggacc gaccgcaagt atcgtccttt ctcccagggg aagtctggtc
     7201 cgtctgggcg ccataccccg ggagagacgg atgatgaacc catccctcac cacagaatca
     7261 tcgcacctgt tgatgatgct ccggtcatgg gtaaggaaaa agtcaacctc ccgactgagg
     7321 ctggccattg ccctgtcgat atcggtgatc atgggatacc cgggcatgtt tgcactcgtc
     7381 atgacaagga gtgggtgcgt gaggtgcgaa aaaaggaggt ggtggaggcc ggtatacgga
     7441 accatacaac cgatggtatg gagattgctg attcctgtat gagccgtccg gtcccgtttt
     7501 tccagaacaa caatcggtct ggttgcactg tcgaggaaca gccgttcctc actagagata
     7561 catgcgatct ggctgacata atcggagttt ctgaccatta tcgcaaaggg ctgttccgac
     7621 cttcccagac gcctcttcaa ttcccccgcg gattcctcaa tgcatgcgat atggaatcct
     7681 ccgattcccc gaattgcaat cagggcaccg gaatcgagaa gccgtgccgt ctcctgtatc
     7741 agatcccggc aatccacggt gtttccgtcg cggtccagca ggatgagatg aggaccgcat
     7801 cgttcacatg cgatggtctg tgcatgatgg cggcggcagg agggatcggc atactcatca
     7861 ccacattcct cacacatcgg aaactgttgc atcacagtcc gttcacggtc atacgggagt
     7921 ttgttgatga tgctgtaacg ggggccacaa ttcacgcacg atgttgccca gtacgacctg
     7981 taccttcccc tgcgggagtc gatatctgca agacactcct cgcaggttgc gatgtcagga
     8041 gggatcatcc cggacatagt cccaggactg ctgggcagga tcaggaagtc tttgggatat
     8101 ggcccttccc agggtgttac agaaaggtaa tcgatccggg caagtggagg gcccgtacgt
     8161 atggaaagga ggaagtcctc aaatgcatcc cccttcgcat aaatttcaac ctcactcccg
     8221 aggttcttca cgtaaccgga aatcccgtat tcccgtgcac gggcatacac aaaaggacgg
     8281 aaacctacgc cctggacaac tccacggacg agtattctcc cgtttcggcg catcggattc
     8341 ctacaaaact ttatggattt actgcgccat atactaagtg ggtttttatc gtgtcaggcc
     8401 atatcaggat agtgaatgat ccggtggaac tgattcccct tctcatcact tttaacaatt
     8461 catgctataa acaggtctat gatcttctca acaagtcatg gatgaccgaa gaggagttga
     8521 gtactcatct gaaggaagag ggggtaagtc actgcatcag catcctgaaa aagggcaatc
     8581 tcattgaaga acaatggagg atgccagagc cgggaaaacg ccctgtgaag gaattccgtg
     8641 cgacatacaa taagttcagg gcgaatttcc aatgcaacct gactgatctc tccgaccttc
     8701 tttatatcgc ggtatccaat gatgagcaat tgcgcatcgt tgttgaaaag gtagaggagg
     8761 aattgtcggg ggggaacaac tccatcaatg atattgcacg caaattcggg gtcagtccca
     8821 cctttatcag gggtctggca aagcggatcc ctcatctgga tgtgaaaggt cagggactgg
     8881 tgctgcttga acgaagccag tgaggatccc ctctcttcca ttctcaggag caagcgtgag
     8941 gtaacccggt tccagatcct tgtcgagatt gctgagcacc agcctgcagt caggcagcag
     9001 gagattgccg acaagctcgg tgtgacccct caggcagtat cagagtacat cagagaactc
     9061 gtcgatgatg gcatggttgc tgcgcacggg agaggacgtt acgatgtgtc ccggtccggc
     9121 atcgaatggg ttcttaacca tgcagaagct cttgaatcgt acgcccggca tatcagaagg
     9181 gatattattc agcaggtttc gacctggacc gctatcgccg cccatgatct taaagagggg
     9241 gaggaggtcg gtgtttatat gaagggaggg tggctctatg cggcgagtga accccagagt
     9301 gcaaccggga tcgtcctgat cgatgcccgt cagggtgatg atgtcggcgt catccggctg
     9361 tcgggaatta tcgatcatac cgaaggagtc attcacgtct gtaaagtgcc acgggtccag
     9421 aggggaggtt cgcagaacgt gaagaaggaa cagctcctgg ccgtcaccag ccaggctgaa
     9481 atggtcgcgg cagtgggcct ggaggcgtac gtggcactgg agaaggcggg aaggatccct
     9541 gacatgttct tcggatcccg tgaaggggtc atcgaggcgg catttcacgg catcgactgt
     9601 gccattttta tcgtggatga agaattcact gattttctga agaggcttga aggggtcggg
     9661 ttatcttacc tcatccatga cctggtcact ccatgagggt tatagtccat ccccagaaag
     9721 gaaattacaa aatcctcttt ctcgacaatc atagtatccg gggaactgcg tttgcagaga
     9781 tatctgaaac cccgaaagga gtgaggcccg ttcaataccg gctcaaatgg ggaagccggc
     9841 agcagtaccg caacactccc acaaaggacc ttatcgcctc cctccggaag agtgaggtat
     9901 tcctcacccg gcgcgacgag atattcggga ggttccttgc agatttccaa atctcgtcat
     9961 cgttggtgaa tctctgccgg atctgtctcc tcgaagagcg gataaccccc ctgagcgata
    10021 aaaccggaat ccgttaccgg ggatctgaat caatctgccc ggagtgtgca aaacgggaaa
    10081 tccgccgtga acttgcacat atgggacgta tcggccgcca gtccatgtcc cacatggaag
    10141 agatgttgtt aaaataccgt gacgtggacc gggttctcgc aagcatccag cctgagcaga
    10201 taacaatgtc ccagacgctc tttgaccggc tggatgccca tccggtccag aagaccgcac
    10261 acctcgaaga gctgcccctg ccccgggtct ttgtggatgc atccggagtc tccagcctca
    10321 tgcccgccca gcagatggcg gtggatgcag gcctgctgta cgggaaggac cttcttgtcg
    10381 tttcagcaac cgcgagcggg aagaccttca tcggggagat ggctgggatg aagaacctga
    10441 tcgaagggag aggacagttc ctcttcctgg tccccctggt ggcccttgca gtccagaaat
    10501 accagcggtt caatgaacga tacggatcat tcactgacgt cgggatcatg atagggaaga
    10561 gcaggctcaa tctgcccgag aaccggcagg caggagacag gaacaccggc gccccggtga
    10621 ttgtcgggac ctacgagggg atcgatcacc ttatcaggat gggatcacgc ctccggcaga
    10681 tcgggaccgt ggtgatcgat gaagtccaga tgcttgagga tcctgaccgg ggacaccggc
    10741 tcgacggcct ggttgcgaga ctgaaatacc tcgcccccaa agcccagttc ctgtatctct
    10801 cagccaccat cggtatgccg aagatcctgg caaagaagct gggggctacc cttgtcagat
    10861 atgatgaacg gcccgtctct ctggagcggt atctcctgtt ccttgagcac aagcagaaga
    10921 tcccgaccga aaagaagctc tgcgaggagg agttcaggac caagtcgtcg aagggatttt
    10981 tcggccagac aatactgttc accaattcaa gggcccggtg tcatgtcatc gccgatgcaa
    11041 tgggacctgg atacgctgct taccatgccg gcctgacggc acaggagcgg agggatgttg
    11101 aggcgaagtt cctgggcggg aagctgaaag cggtgatcac gactgcagcg ctcggtgcag
    11161 gggtagactt ccccgcatcc caggtcattt ttgattcgct tgccatggga atctcgtggc
    11221 tcaccgtcca ggaattcaac cagatggcag gaagggcagg caggcctgat ttccatgatc
    11281 tcgggaaggt ggtcatcctt gcagagcctg gcgggacgta tggaagaggg tcctcattca
    11341 ctgaggaaga ggtagcgatg cggctcctca agggggagat ggaggaagta gcgccagagc
    11401 atgacctcga gcagagctct gaagagtatg tggccaatgc cgttgtctgc aggggagacg
    11461 agcaggatct ggaatacatc aataaccaga tgatcgggac ccttgaaccc gttctccccc
    11521 tcctcctcga aaagggaatg gtccgccaga ccaacggaag gatcgaactg atgcccctcg
    11581 cccgggtgat ggccgaacat ttcatcggag tggaacgtct gctcgagatc ctcaccctct
    11641 caaacaaatt caaggatccg atcgagatcc ttaccgaact cgaatgcagc gccctggaag
    11701 aacgaaagaa agagaaggga aagggaaaga gagagcagcg ctgatccacc gcctcctcgt
    11761 aacaatagac cgtggaactt ttttcaggag gatatactgc agatccaccg gtcgtatatc
    11821 ggatcccgcg tattctcgat aatttctgat tctttgccat actgatctgc ccagttcctg
    11881 attcgcatgg cctcctgctc ggtccctgtc gtgataaggc tgcgatcagt cagcatgatc
    11941 agttccccag taatctgctc ccacatcccc tcattgaaat catggatctc cccgaacatc
    12001 agcccaagcc cggtatcgca atggcccaca tagtggtgaa ggagggaggc atcaatcagc
    12061 atcgtctcct cctgcagaag cctgcccctg gcaaatccga gggagatcag agacggatca
    12121 ttgtcgcagg agaagatttt atacccccgt ttccggagta cgagggatcc gactcctgat
    12181 ccgcatccgc agtcaaggca cgatctcccc gctccgtctc ccaggatctc gccaacaagg
    12241 gagtcaacga gactcacccg tccgggattc agatcttcaa gggcgggagt ggtatggagt
    12301 gctagttccc tgctgtaata gccccggact gcagcttccc agataccccg ctcctcctgg
    12361 aagatatcgc ctccgagata ttcgaatcgg gcgatcagtg caccagtcgg tgggcggagg
    12421 tgaaaacagc cggcaatcca ggttccatct tccctgcata ctgccatgca gagcggatct
    12481 gaatcctggt caacaagaag ccgggcctgg gtaccttcgg attcttcgag gagtgcagtg
    12541 aacgtggtat ccgggagtag tgcgaaagaa ggttcaatca gctgcacctc cacaaggccg
    12601 aggatctctg cgatgtcctg catctcttca ggccccgtat tttttggcac ggttgatcag
    12661 gtcgcagatg attggcatca gcgaatggcc tgttatccgg ttgagtccgc cctgggcaca
    12721 ggcaacctca tcgaactgca tgaccgcatc ggtccggaca tcatctcccc agatgacaag
    12781 cggaacaggg tcagcactgt gatcccggat gctgcagggg gtgctgtggt cagcacagat
    12841 gacgatcagg cattcatcgg tatacagcag cggttcgagc gcagcatcaa tatgctcgat
    12901 gaagttcttc ttctcgatgg catggccatc atgccctgcc tcgtcagccc ccttgatatt
    12961 gagcagcaca aaatcctgtt ctttcagctc ggtaagggca gccttcactt ttccttcaag
    13021 gtttgtatca gacgaacccg tcgctccttc caccgggacg tacctgagtc cgagagattt
    13081 cccgatcccg gttatcaggg tcgcagcgga tatgacactg ccggagagac cataccgctc
    13141 gctgaaaggt tcatacgtgc ccatctgccc tgcaccacgg acgaggatca tgtttgccaa
    13201 aggcagcccc ctcttccttc gatcctggtt cagggtatgt ttcgcaagga ttgggagtga
    13261 ctgccgtata aattcattca gtgcctctgt ggtcttcctg tcggctgctg aatcacggag
    13321 cgggaggaac gctggtggtt ggatcccctc tttcttcggg tcgtttgagg agacgcaggc
    13381 tccgagcccc gctcccctga aggcgagcgc cgcgcgatgg cctgccccgg aacggaagag
    13441 cacctccacc ccga
//
LOCUS       DAOOCO010000033        13197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4340_length_13197_cov_5.019784, whole genome shotgun sequence.
ACCESSION   DAOOCO010000033 DAOOCO010000000
VERSION     DAOOCO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_13197_cov_5.019784"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PK154_03225"
     CDS             complement(<1..1408)
                     /locus_tag="PK154_03225"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPW10107.1"
                     /translation="MVLFPERIRFFDTTLRDGEQTPGVSLKPVEKLEIATRLSEIGVH
                     VIEAGSAVASEGERQALRLISDAGLPAEICTYVRAVPLDIDYAADYGADSVHLVIPVS
                     DLHIEKKLRKSRDEVYAMAMDAVTYAKERGLIVELSGEDASRADPTFLKSVYSGGLDH
                     GADRLCFCDTVGLLTPERVAVIIPPLCLAPLSIHCHDDLGMGLANTMAALHAGASCAH
                     VTVNGLGERAGNTPFEELVMALEILYQYRTGIRTAEIYPLAALVSRLTGVPLPTNKAI
                     VGEMAFTHESGIHAHGVLKEPSTYEPVPPERIGRKRRIMLGKHSGSASVEAALTELHY
                     HPDKKQLKEILGRIKDLGDKGMRVTDSDLMAIAEAVMAIECVPVLKMKQFTVVSGSNV
                     IPTASVTLLVKDEEVTSASTGTGPVDAAMEALRKSVAAVADISLEEYHVDAIHGGTDA
                     LVDVTVRLSKDGKIITSRG"
     gene            complement(1625..2347)
                     /locus_tag="PK154_03230"
     CDS             complement(1625..2347)
                     /locus_tag="PK154_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HPW10108.1"
                     /translation="MEYGSIIGESIEYAKEAVWEKWTKWILLIVSTIIFPLILGYVME
                     IYRGTKPAPELEHWGKLFIDGLKLIAAWIVYMLPVIAVLVVFGGWALFSAIQQSAMSG
                     NPDFFTSNPELLMPLIATFLFGLLIAVILAILISIVAYIGIIRMARAERFGEAFNFSA
                     IFETIRKIGWGSYILALIILVVVVMVFGIILALIMAIPYIGWLIYLVLLPLLSIFQAR
                     YLAQVYDAGEDQTIESAVPVQA"
     gene            complement(2639..4123)
                     /gene="purH"
                     /locus_tag="PK154_03235"
     CDS             complement(2639..4123)
                     /gene="purH"
                     /locus_tag="PK154_03235"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPW10109.1"
                     /translation="MKWALLSVWDKQGIVDLAKTLSAAGVKILSSGGTGTVLEEAGID
                     FTEVSAYTGSTEMMDGRVKTLHPKVHGGLLGRRGIDDATMAEHGIAPIDLLVANLYPF
                     EQMALTIPDLDRLVEYIDVGGPAMIRAAAKNYRDVAVITDPGDYPLVADALKKGGFSP
                     EQRFMLARKAFARTAAYDAAISNYFQSSGAECAPVITLQYRNGRQLRYGENPHQKGVV
                     YGEAGIAAVAPLQGKQMSYNNYLDLNAAAGLLNEFEDPAAVIVKHNNPCGVSLGAHVL
                     EAYIGAREVDPVSAYGGVVAINGEVGLPLAEEICSTFVEVAAAPAFTPDALDTMHRKE
                     NMRVVVLPHPSGGPEFRTIAGGILMQETPPYREHWQVVTDRDATPAELRALQMGWKVC
                     KHTRSNAIVFASQQKILGIGAGQMSRVDSAKIAIDKSLFSLEGSVVASDAFLPFPDTL
                     EIAAAAGATALVQPGGSIRDDEVIAVANNLNMAMVFTGIRYFRH"
     gene            4273..4794
                     /locus_tag="PK154_03240"
     CDS             4273..4794
                     /locus_tag="PK154_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPW10110.1"
                     /translation="MVHEKEEEFNGLDQEKKELSASDSPTPSLEDLKRDYDELNDRFL
                     RLAADFENFRKRTTREMDAQARFAIEGFAIELLEVADNFERALKSGDTTAREGLEQIQ
                     KLFRTILERHGIQAIESVGTKFDPVCHDAVVSLPSDCEEGTVVEEFTCGYRMHDRVIR
                     CAKVAVSKGTEGD"
     gene            4796..6634
                     /gene="dnaK"
                     /locus_tag="PK154_03245"
     CDS             4796..6634
                     /gene="dnaK"
                     /locus_tag="PK154_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPW10111.1"
                     /translation="MAKEKVLGIDLGTTFSCMAIMEAGTPVVIPNAEGGRTLPSVVAF
                     TKDGERLVGSLARRQAITNPTRTIQSIKRKMGSTEKIKIDDKSFTPQELSAMILQKLK
                     VDAEAYLGEKITKAVITVPAYFNDAQRQATKDAGKIAGLDVLRIINEPTASALAYGID
                     KEKDATVLVYDLGGGTFDVSILTLGDGVFEVKATSGNNFLGGDDFDKRVTDYLVEEFR
                     KKEGIDLRTDPIAMQRLRDASENAKIELSQKMKTTINLPYITTGPSGPKFLDIDLTRA
                     KLEQLIGDLVESTVQPVRQALADAKMKPEDIHHVLLVGGSTRVPLVQETIKKILHKEP
                     DKGINPDECVALGAAIQGAVLTGEAKDIVLLDVTPLTLGIETLGGIATKLIERNTTIP
                     TRKSQIFSTAADGQTSVEIHVFQGERALAKDNFTLGRFQLSGIPPAPRGIPQIEVTFD
                     IDANGIIHVSAKDLGTGNQQAITIKGDRRLSEDEIKRMVNDSKNFEEEDKKKKEEIEL
                     RNLADSAVFNAEKMIKESGDSMEPADREKVQAGIDAVRGALQSDNTEEIKKQMEALTE
                     AVYAVTTKIYQKAQAERQSAEAAGGGKGGTENDDTVVDADYSMKDE"
     gene            6640..7764
                     /gene="dnaJ"
                     /locus_tag="PK154_03250"
     CDS             6640..7764
                     /gene="dnaJ"
                     /locus_tag="PK154_03250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW10112.1"
                     /translation="MATGDYYEILGIPRTADENEIKKAYRSLARKYHPDVCKEQGAEE
                     RFKQINEAYSVLSDAQKRAQYDHLGHDSYTSASKGSYTGGGGGFGGFSADFSGFGDIF
                     DFFGGGFGGQRPSGPRPGADLMMNLWISLEDAVFGTDREIEVSHAEPCTACDGTGSST
                     KKRISCPRCGGSGQLRQMSQSIFGQFVRMTPCPDCRGAGKVPESRCGTCRGAGITQVK
                     RKVTVRIPAGIDTGMRLRLEGYGEAGDIGAPNGDLFIEVSVKPDERFVRKGDNLETAA
                     EITPAQAVMGSEIEIETIGKKKVDLTIPPGIQHDTALKIPGEGVRRRGRPGDLLVRVK
                     IRIPRQIPPEAKELYEKILEIEGAKSGHKKGFFSGLMGKG"
     gene            7856..8812
                     /locus_tag="PK154_03255"
     CDS             7856..8812
                     /locus_tag="PK154_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW10113.1"
                     /translation="MRLIVELSGEHPELPFAELQVVGEVIDKRPQVAVVESRDPWSAR
                     RLAMAHSVLEYLGDAPADPDAFLSMVRDLGITADCPFAARVKKIGGSAWNVTVPDLER
                     RIGSIIRGTVDLDDPGCEYRAILSGDRCYFGKVLFHGERPSYELRRPGSRTFFHPGVM
                     MPRLARALVNISLVAPGERLLDPFCGTGGIVLEAVLVGARGIGSDIDPFMVSGSRKNV
                     GGAEFVQAACGALPFGDACIDAVVTDLPYGQSVSIVAGSLETLYDDALLEMRRVLLPG
                     KRAVVVTHRDISRQAAGILSIRACYAQRVHKSLTRRILVLER"
     assembly_gap    8898..8997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8998..9722
                     /locus_tag="PK154_03260"
     CDS             <8998..9722
                     /locus_tag="PK154_03260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPW10114.1"
                     /translation="GIRMENEDACGVNHFRHPLGTVVLLAVADGLGGHPAGEVASALA
                     VDTIIKVTEERLALLEDIAVESLKRLLEFAFMEANNAVIAIGERDERCRGLGTTLVAA
                     LVTENDTCIVGHVGDSRAYLVSRDLHRITRDHSRVQELVDEGILTPAEAECHPMRHII
                     TRIIGRRGEMPDITICRLDGSRLLLCSDGLLDGMGEPAILTAMQGAGMPEVCDLLVER
                     AREKSRDNITVVVAERKGSNGW"
     gene            complement(9740..10441)
                     /locus_tag="PK154_03265"
     CDS             complement(9740..10441)
                     /locus_tag="PK154_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPW10115.1"
                     /translation="MQTSEYPRASDALKKALGLTSSPVAIRIVQKKEEIPSGVEKLDK
                     TVRHCQMVSFARKEGRIFYSTVDNHECVGGAWALGLREISESLKSGDFYFKLGKFETP
                     AACKRTIDQIPHLESGSTYATMYAPLEKAPFIPQVILIVAPPRVMLKLAQATLYQLGG
                     RVHSHFAGIQSVCADTTTQTYLSGTANYSLGCDGSRKFSGIEDSEMVMGFPAEMLPQM
                     VQAVEIVTAAPGSKK"
     gene            complement(10507..11319)
                     /locus_tag="PK154_03270"
     CDS             complement(10507..11319)
                     /locus_tag="PK154_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPW10116.1"
                     /translation="MKCMMVSRIDDEKAIEYTGKLGDALCRGGIDVEFETATAHGLHR
                     EGSTLSKARPDMAVIVGGDGTILRTVQAMDRQVPVIGINWGEVGFLADLEPEEAEGVL
                     TTISDGFLVEPRMRLSFFVGDSHIGDALNEVLIVTSRPAKMLRFGVIIDGILSERFRA
                     DGLLVSTPTGSTAYAMSAGGPIIDPRIEGALLVPLAPYMLSSRPHFISGDRPLEIRLE
                     SAKPASLVIDGQKSMDLGCEAAIRVKKSTTPALFVDVGRNFFAKVDQKLRRL"
     gene            complement(11324..12100)
                     /locus_tag="PK154_03275"
     CDS             complement(11324..12100)
                     /locus_tag="PK154_03275"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HPW10117.1"
                     /translation="MDFLAVCEHIAHDVAEAIDPLIGTPDAGRNIRMGADGTPTKHID
                     LVAEDCVLAHLAGHPLCRTLISEEAGKVAMEGEHGTIFLDPIDGTYNAVAGIPFYALS
                     IAYAENGYMKQAFVRNLAGTETFTARAGEGAFLDRRRISVSRTASLDESALSVYGRKF
                     DPGRAMQLGQKIRRWRLLGASALELCYTGCGRIDGFIDLRGTLRVTDAAAGILICTEA
                     GGRVSDLDGEPLVFPDEVTIGRCMVATNGVMHRKVIEYLR"
     gene            12184..12567
                     /locus_tag="PK154_03280"
     CDS             12184..12567
                     /locus_tag="PK154_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HPW10118.1"
                     /translation="MKEQTEVGKLKEGRYVVIDEEPCKILGISVSKPGKHGAAKARID
                     AVGIFDGVKRSIVQPVSAKTYVPIVERKSGQVITIAGTTATLMDMKEYTNFEIEIPSD
                     KAGQVEVGKEIPYIESLGKKKLDLD"
     gene            complement(12650..13087)
                     /locus_tag="PK154_03285"
     CDS             complement(12650..13087)
                     /locus_tag="PK154_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPW10119.1"
                     /translation="MSGSTSWNFPPPIPDGANTSPRIRLSGLNAVSVAVMAVNPFQPS
                     CCSFCPWLIWNIPAGPEIPAGIPPEGIVSFPITAVQGMNDFGRIGYSGPCPPQGTTHR
                     YALKVYGLDAMLTLPPGATKAGLITAMRSHVLQYGETAALYSR"
BASE COUNT         2895 a         3639 c         3866 g         2697 t
ORIGIN      
        1 cgccgcgtga cgtaattatc tttccgtctt tacttaatct taccgtcacg tccacgagcg
       61 catccgtccc gccgtggata gcgtcgacat ggtactcttc cagcgatata tcggcgactg
      121 ctgcgactga ctttcgcaac gcttccattg cggcatcgac cggcccggtc ccggttgatg
      181 cactggtgac ctcttcatcc ttgacaagga gggtgaccga tgccgtcggg ataacattgc
      241 tgcccgacac caccgtgaac tgcttcattt tgaggaccgg tacgcattct atcgccatca
      301 cggcttcggc gatggccata aggtcgctgt cggtgaccct catccccttg tcccccagat
      361 ccttgatccg gccaaggatc tctttcagct gcttcttgtc cggatggtaa tggagctcgg
      421 tgagtgctgc ctccactgag gcagaccccg agtgcttccc gagcatgatg cgccgttttc
      481 tcccaatccg ttccgggggg accggttcat aggtgctcgg ttctttcagc accccgtggg
      541 catggatccc gctctcgtgt gtgaaagcca tctcgcccac gatcgccttg ttcgtgggga
      601 gcgggacccc ggtaagacgg gataccaggg cagcaagcgg gtagatctct gccgtcctga
      661 tcccggtccg gtactggtag aggatctcaa gcgccatgac gagctcctca aagggggtgt
      721 ttcctgcccg ttctccaagt ccgttgacgg tgacatgagc acatgaggct cctgcatgga
      781 gtgctgccat cgtgtttgca agccccatgc ccaggtcgtc gtggcagtgg atggagagcg
      841 gggcgaggca gagcgggggg atgatcaccg cgacccgttc cggagtgagg agacctacgg
      901 tatcgcagaa acagagccgg tcggcaccat ggtcaagccc cccggaatag accgatttca
      961 ggaatgtggg atctgcccgg gaggcgtcct ccccggagag ctccacgatg aggccccgct
     1021 cctttgcata cgtgacggcg tccatcgcca tcgcgtacac ctcgtcccgg ctcttccgga
     1081 gtttcttctc aatatggaga tcgcttaccg ggatgacgag atgcaccgag tcggcaccgt
     1141 aatctgcggc atagtcgatg tcaagaggga ctgcacggac gtacgtgcag atctcggccg
     1201 ggagcccggc gtccgagatg agccgcaggg cctggcgttc cccttcagaa gcaaccgcgg
     1261 atcccgcctc gatcacatgg acaccgatct ctgagaggcg cgttgcaatc tccagtttct
     1321 caacaggttt caacgagaca ccgggagtct gttccccgtc ccgtaacgtg gtatcaaaaa
     1381 agcgaattct ttcagggaat aaaacgatct gtcatggata atccaccaca ccatactgtt
     1441 gctgcagcgc ctcattaata tatcggacca agaaaaatgg actttttaag gaaaaacaga
     1501 cgatcatgtc attttttatt gctcttacgg accctgcctg tcgcgctcca aaacataaca
     1561 tgattctttt gagggccaca agcataaaag ttgaaaatgc cggaaaaaag ggatttgaaa
     1621 aaaattatgc ctggacgggg actgcactct caatcgtctg gtcctctccg gcatcgtaca
     1681 cctgggcgag ataccgtgcc tggaatatcg agagtagcgg gagcagtacc aggtagatga
     1741 gccagccgat gtacgggata gccatgatca gggccaggat gatgccaaag accatgacca
     1801 ccacgacaag gatgatcaac gcaaggatat agctccccca cccgatcttc cggatcgtct
     1861 caaaaatcgc agagaagttg aaggcctcgc cgaaccgctc cgctctcgcc atgcgtatga
     1921 tgccgatata ggcaacgata ctgatcagaa tcgcaagaat caccgcgatc agaagcccga
     1981 acaggaacgt ggcgatgagc ggcatgagaa gttcaggatt cgaggtaaag aagtcgggat
     2041 tcccgctcat ggcagactgc tggatggctg agaagagcgc ccagccgccg aataccacca
     2101 gtaccgcgat gacagggagc atatagacaa tccaggccgc gatcagcttc aggccgtcaa
     2161 tgaagagttt cccccagtgt tcgagctcag gggcaggttt cgtgccccgg taaatttcca
     2221 ttacatatcc gagtatcagg ggaaagatga tggttgatac aatcagaagg atccacttcg
     2281 tccatttttc ccatactgct tcctttgcat actcgattga ttcgccgata atcgaaccgt
     2341 attccatcga tatcacctgt tagatgatat caagtagggt ccttaaaaaa tataccacag
     2401 ctccgaacca cagcatccgg accctcatgg cagctcatag ggcttctctt ccgggaagga
     2461 tcaaacgcgc gtggagggaa tgcaattgga gggggcattc gggcgattca agaaaatgga
     2521 tgattgacag gattgacccc tgcagagagg aactctgact caatgaatca ggatttatca
     2581 tcctgttcaa aaaaaatctg gtgaccgaat tatccctgat gcaggaacat acccgatcct
     2641 aatgcctgaa gtaccggatg ccggtgaaga ccatcgccat gttcaggttg tttgccactg
     2701 cgataacctc atcatccctg atcgacccgc caggctggac cagtgcggtg gcacctgccg
     2761 cagccgcaat ctcgagagtg tccgggaagg ggaggaacgc atcggaagcc acgaccgatc
     2821 cttcgagcga gaagagggac ttgtcgatcg caatctttgc cgaatccacc cggctcatct
     2881 gtcctgcgcc gatccccagg atcttctgct ggctggcaaa gacgatcgca ttgctccggg
     2941 tgtgcttgca gaccttccat cccatctgca gcgcccggag ctcggcaggg gtcgcgtccc
     3001 gatcggtgac gacctgccag tgctcccggt agggcggggt ctcctgcatg aggatgcccc
     3061 ctgctatcgt cctgaactca gggccaccgc tggggtgagg caggacaacc acccgcatat
     3121 tctccttccg gtgcatggta tcaagggcat cgggggtgaa cgcaggggca gctgccacct
     3181 ccacgaaggt cgaacagatc tcttcagcga gagggagccc tacctccccg ttgattgcaa
     3241 cgactcctcc gtatgccgat accgggtcga cctctcttgc tccaatatat gcctcgagta
     3301 cgtgtgctcc gagggagacc ccgcacgggt tattgtgctt gacgatcacg gcagcaggat
     3361 cttcgaattc gttgaggagg ccggctgctg cgttcaggtc gagatagttg ttgtaggaca
     3421 tctgctttcc ctggagcggt gcaaccgcag cgattcccgc ctcaccgtac accacgccct
     3481 tctggtgtgg attctcgcca taccggagct gtcgcccgtt cctgtactgg agggtgatga
     3541 ccggggcaca ttccgcgcca gaggactgga aatagttgct gatcgccgca tcgtaggctg
     3601 ctgtccgtgc aaacgctttt ctggccagca tgaaccgctg ttcgggggag aaccctcctt
     3661 tcttcagggc atcggcaaca agcgggtaat cgcccgggtc cgtgatcacc gcaacatccc
     3721 ggtagttttt ggccgcagcc cggatcatgg ccggccctcc cacatcgata tactccacca
     3781 gcctgtcaag gtccggaatg gtcagggcca tctgctcgaa cgggtacagg tttgccacca
     3841 gcaggtcgat cggggcgatc ccgtgttctg ccattgttgc gtcatcgata cccctccgtc
     3901 caaggagccc cccgtgcacc ttggggtgca gggtcttcac ccggccgtcc atcatctcgg
     3961 tcgacccggt gtatgctgag acctcggtga aatcaatacc cgcctcttcc agcaccgtgc
     4021 cggttcctcc tgatgagagg atcttcaccc cggcagcact gagtgtcttt gccagatcca
     4081 cgatcccctg tttgtcccag acagaaagga gtgcccactt catggtcagg gtattatgag
     4141 atgatctgat atgattgtga tggagtgctg gcggggatgg tcctgccaca gcctgctgcc
     4201 gttggcttca gacgttgcat cgatgaaaat gattttatag aaccacaatg caataattat
     4261 cctgatcgtc ttatggttca cgaaaaagag gaggaattta atgggctgga tcaggaaaaa
     4321 aaagaattat cagcctccga ttcacccact ccctcccttg aggatctgaa gcgtgactat
     4381 gatgaactga atgaccggtt cctgcggctt gctgccgatt tcgaaaattt caggaagaga
     4441 acgacgcggg agatggatgc acaggccagg tttgcgatcg agggttttgc gatcgaactc
     4501 cttgaggtcg ctgataattt cgaacgggcg ctcaaatcgg gtgataccac tgcccgggag
     4561 ggtctcgaac agatccagaa gctcttccgg acgatcctcg aacggcacgg gatacaggca
     4621 atcgaatccg ttggaacgaa gttcgatccc gtctgccatg acgcggtggt ctccctcccc
     4681 tctgactgtg aagaagggac agttgttgag gagttcacct gcgggtacag gatgcatgac
     4741 cgggtaatac gctgcgcgaa agttgcagtc tcgaagggaa cagaaggtga ctgatatggc
     4801 aaaggaaaaa gtgcttggta ttgatcttgg aacaacgttc tcctgcatgg caatcatgga
     4861 ggcagggacc cctgttgtca tccccaacgc agaagggggt cgtacactcc cgtccgttgt
     4921 tgccttcacc aaagacggtg aacggcttgt cgggagcctt gcccggagac aggcgataac
     4981 aaacccgacg aggaccatcc agtccatcaa gaggaagatg ggctcgaccg agaagataaa
     5041 aatcgatgac aagtcgttca ccccccagga actatcggcg atgatcctcc agaagctgaa
     5101 ggtggatgcc gaggcgtatc tcggggagaa gatcacgaaa gctgtcatca cggtccctgc
     5161 ctatttcaac gatgcccagc ggcaggcaac caaggatgcc gggaagattg ccgggcttga
     5221 cgtgctccgg atcatcaatg aaccgacagc gagcgctctc gcctatggga tcgacaagga
     5281 gaaggatgcc actgtgctcg tctacgacct tggaggcggg acgttcgatg tatcaatcct
     5341 gaccctcggt gatggtgtat tcgaggtcaa ggcaacctcc gggaataact tccttggagg
     5401 ggatgacttt gacaagaggg tcacggacta cctggttgag gagttcagga agaaggaagg
     5461 gatcgacctc aggaccgacc cgatcgccat gcagcggctg cgggatgcct cggagaatgc
     5521 gaagatcgag ctctcccaga agatgaagac caccatcaac ctcccctaca tcacgacagg
     5581 tccgagcggc ccaaaattcc ttgatattga cctcacaagg gcaaagctcg agcagctgat
     5641 cggcgacctg gtcgaatcca cggtccagcc ggtccggcag gcccttgccg atgccaagat
     5701 gaaacccgag gacatccatc atgtcctgct ggtgggaggg tcaacccgcg tccccctcgt
     5761 ccaggagacg atcaagaaga tcctccacaa ggaaccggac aaggggatca atcccgatga
     5821 gtgtgttgca ctcggggcag caatccaggg cgcagtcctc acgggagagg caaaggacat
     5881 cgtgctcctc gatgtcaccc cactcaccct cgggattgag accctcggcg gcatcgcgac
     5941 caagctgatc gagcggaata ccaccatccc cacccggaag agccagatct tctcgaccgc
     6001 agcagatggg cagaccagtg tcgagatcca tgtcttccag ggtgaacgtg cccttgcaaa
     6061 ggacaatttc acgctcggaa ggttccagct gtcagggatc cctcctgcgc cccgcggaat
     6121 cccccagatc gaggtgacgt tcgatatcga tgcaaacggg atcatccatg tctcagcaaa
     6181 ggatctcggg acagggaacc agcaggccat caccatcaag ggcgaccgga ggctctctga
     6241 agacgagatc aagcggatgg tgaacgattc caagaacttc gaggaagagg acaagaagaa
     6301 gaaggaggag atcgagcttc gcaaccttgc tgacagcgct gttttcaacg ctgagaagat
     6361 gatcaaggag agcggggact cgatggaacc ggcagaccgt gagaaggtcc aggcagggat
     6421 cgatgctgtc cgcggagccc tccagtccga taataccgag gagatcaaga agcagatgga
     6481 agccctcacg gaagcggtct atgccgtgac taccaagata taccagaagg cacaggctga
     6541 gcggcagtca gcggaggctg cagggggagg aaagggcgga acagagaacg atgataccgt
     6601 ggtcgatgcc gattacagca tgaaagacga gtgagcccga tggccaccgg ggactattat
     6661 gagatcctgg gtatcccgag gaccgctgat gagaacgaga taaagaaagc ttaccggagc
     6721 cttgcccgga aatatcatcc ggacgtctgc aaggagcagg gggcggaaga gcggttcaag
     6781 cagatcaacg aggcatacag cgtcctttcc gatgcccaga aacgggcgca gtatgaccac
     6841 ctcgggcatg atagttacac cagtgcatcg aaaggctcct atacgggggg aggaggcgga
     6901 ttcgggggat tctcagcgga tttctccggt tttggcgata tcttcgattt ttttggtgga
     6961 ggattcgggg gacagcgccc gtcaggccca cgccccggtg ccgacctgat gatgaacctc
     7021 tggatctcgc ttgaggatgc ggtctttggg actgatcggg agatcgaagt ctcccatgcc
     7081 gaaccctgca cggcatgtga cggcacgggg agcagcacca agaaacggat ctcctgtccc
     7141 cgctgcgggg ggagcggcca gctccggcag atgagccaga gcatcttcgg ccagtttgtc
     7201 aggatgaccc cctgccctga ctgccgcggt gcggggaagg tccctgaatc acggtgtggt
     7261 acctgcaggg gagccgggat cacccaggtg aagcggaagg tgaccgtccg gatccctgcc
     7321 ggcatcgaca ctgggatgcg cctgaggctg gaagggtacg gagaagcagg ggatatcgga
     7381 gctcccaatg gcgacctttt catcgaggtc tcggtgaagc cagacgagcg gtttgtccgc
     7441 aagggagaca accttgagac agcagcggag atcacgccag cccaggcggt catggggtcc
     7501 gagattgaga tcgagacgat cgggaagaag aaggttgacc tcaccattcc ccccgggatt
     7561 cagcatgata ccgccctgaa gatccctggc gaaggggtcc gccgccgggg gaggcctggc
     7621 gatctcctgg tacgggtcaa gatccggatt ccccggcaga tacctcctga agcaaaggag
     7681 ctctatgaga agatcctcga gattgaaggg gcaaaatccg gccataagaa ggggttcttc
     7741 tcaggactca tgggcaaggg ctaaaatccg gaaaacctcc cctatccacg cgctcctttt
     7801 ccggcagatc cgctgccacc aggtttattc ccccggatag ccactgtact ggaacgtgag
     7861 actgatcgtt gagctatcag gcgaacaccc cgaacttccc tttgccgagc tccaggtggt
     7921 gggggaggtt atcgataaaa gaccccaggt tgcggtggtg gagagcaggg atccctggtc
     7981 agcccgccgc cttgccatgg ctcattcggt cctcgagtat ctcggtgatg ctccggctga
     8041 tccggatgcc tttctctcaa tggtccggga cctcgggatt accgcggatt gcccctttgc
     8101 ggcacgggtc aaaaagattg gtggttcagc ctggaatgtc acggtccccg atcttgaacg
     8161 gcgcattgga tcgataataa ggggaacggt ggacctggat gatcccggct gcgagtaccg
     8221 ggctatcctt tccggggacc ggtgctattt tgggaaggtg ctcttccatg gggaacggcc
     8281 gtcttatgaa ctgcggagac ctggcagccg caccttcttc catcccggag tgatgatgcc
     8341 ccgcctggcc agggcactgg tcaatatctc tcttgtcgct cccggagaga gactgcttga
     8401 tccgttctgc ggaacggggg ggattgtcct tgaagcggta ctggtaggtg ccaggggcat
     8461 tggaagcgat atcgacccgt tcatggtctc cggcagtcgg aaaaatgtag ggggtgcaga
     8521 attcgtgcag gctgcgtgcg gggctcttcc ctttggcgat gcatgcatcg atgcggtggt
     8581 caccgatctc ccctacggcc aatccgtctc gatcgttgca gggagcctgg agacgctgta
     8641 cgatgatgcc ctcctggaga tgaggcgggt tctcctgccc ggaaagaggg cagttgtagt
     8701 cacccaccgg gatatctcgc ggcaggctgc cgggatcctc tcaatccggg cctgctatgc
     8761 ccagcgcgta cacaagagcc tgacacggag gatcctggtc ctcgaacgat aagagcctgg
     8821 tttttctttt ccgataaagg ataaatccgt gccggacaag atattcttta catattcagt
     8881 gatccggtgg atcattcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg
     9001 gaatcaggat ggaaaatgag gacgcctgtg gcgtgaatca tttcagacac cccctgggca
     9061 ctgttgtcct gcttgcagtt gccgacggcc ttggtggcca cccggcagga gaagtggcaa
     9121 gtgcccttgc cgtggatacc atcatcaagg tgacagaaga gcgtctcgcc ctcctggagg
     9181 atatcgcggt cgaatccctg aaaaggctgc ttgaatttgc cttcatggag gcgaacaacg
     9241 cggtcattgc gatcggtgaa cgtgatgagc ggtgccgcgg gcttggcacc accctggttg
     9301 ccgcactggt gaccgagaac gacacctgca tcgtggggca tgtcggggat tcccgtgcgt
     9361 acctcgtctc ccgggacctg caccggatca cccgcgatca ctcaagggtc caggagcttg
     9421 ttgacgaggg gatactcacc cctgccgagg cggaatgtca tcccatgagg catatcatca
     9481 cccgtatcat cgggcgaaga ggggagatgc ccgacattac gatatgccgg ctggatggat
     9541 cccgccttct cctctgctcc gatggtctcc tggatgggat gggggagccc gcgatcctca
     9601 cggccatgca gggggcaggg atgccggagg tctgcgatct gctcgtagag agagcaaggg
     9661 agaagagccg ggacaatatc acggtcgtgg tggctgaacg gaagggcagc aacggctggt
     9721 aaaagaaagt ggtcggtttt tatttcttgg atccgggggc agcggtgaca atctccacgg
     9781 cctgcaccat ctgggggagc atctcggcag ggaatcccat gaccatctcg ctatcctcaa
     9841 tgccggagaa cttccgcgat ccatcgcacc cgagcgagta gttggcagtg ccggagagat
     9901 aggtctgcgt ggtcgtatcg gcacagaccg actggatccc ggcaaagtgg gaatggaccc
     9961 gcccgccgag ttggtacagc gttgcctgtg caagcttcag catgacccgg gggggtgcaa
    10021 cgatgaggat cacctggggg ataaagggag ctttttcaag cggtgcgtac atcgtcgcgt
    10081 aggtggaccc tgactcgagg tgagggatct ggtcgatcgt ccgtttgcag gcggcaggag
    10141 tctcaaattt ccccagcttg aagtaaaaat cgcctgattt caggctttcc gatatctccc
    10201 ggagcccgag ggcccatgcc ccgcctacgc attcgtggtt gtccacggtc gagtaaaata
    10261 ttctgccttc ctttcgggca aaggatacca tctggcagtg ccggaccgtc ttgtccagtt
    10321 tctcgacacc agaggggatc tcttctttct tctgcacgat gcggatggcc accggtgagc
    10381 tggtgaggcc aagtgccttt ttgagggcat ccgatgctct gggatattcg cttgtctgca
    10441 tggatcattc tccttcgtgg atacggtatg ctcgcattgc gagctgataa aaatgtggaa
    10501 agtgacctag agccggcgga gtttctggtc gacctttgca aaaaaattcc ttcccacgtc
    10561 aacgaagagt gcgggagtgg tggatttttt taccctgatg gccgcctcgc agccgaggtc
    10621 catgctcttc tggccatcga tcacgaggct ggcgggtttg gcactctcca ggcgtatctc
    10681 gagggggcga tcaccgctga tgaaatgcgg ccgtgaagag agcatgtaag gggcaagggg
    10741 gacgagcaga gcaccctcga tgcggggatc gatgataggg ccgccggcac tcatcgcata
    10801 ggcggtagac ccggtcgggg tgctgacgag caggccatca gccctgaacc gttcggacaa
    10861 tattccgtcg atgatcacac cgaacctgag catctttgcc ggacggctgg tgacgatgag
    10921 gacctcgttg agtgcatcgc cgatatggct gtcaccgaca aagaacgaga gcctcatcct
    10981 cggctccacc agaaatccat ctgagatggt ggtgagaacc ccctctgcct cctctggttc
    11041 aaggtcggca aggaatccca cttcgcccca gttgatgccg ataaccggca cctgccggtc
    11101 catggcctgg acggtgcgga ggatcgttcc atctcctccc acgatgacgg ccatatccgg
    11161 gcgggctttt gagagggtgg aaccctccct gtggagcccg tgggcagtcg cggtctcaaa
    11221 ttcgacgtcg atcccccccc tgcacagagc atctccgagc ttcccggtgt attctatggc
    11281 tttctcgtca tcgatccggg ataccatcat gcatttcata gtctcaccgc aggtactcga
    11341 tcactttccg gtgcatcacg ccgtttgtgg cgaccatgca cctgccaatc gtgacctcgt
    11401 cgggaaatac gagtggttcc ccatccaggt cgctcactct ccctcctgcc tctgtgcaga
    11461 tcaggatccc ggctgctgca tcggtaaccc ggagggtccc ccggaggtcg ataaacccgt
    11521 cgatcctccc gcagccggta tagcagagct cgagtgcgga tgccccgagg agtctccatc
    11581 gccgtatctt ctgcccgagc tgcatcgccc tccccgggtc gaatttcctg ccatacaccg
    11641 agagcgcgct ctcgtcaaga gacgcggtcc tcgagacaga aatccgcctc ctgtcaagaa
    11701 acgccccttc gcctgctctc gcggtgaacg tctctgtgcc cgcaaggttc ctgacaaatg
    11761 cctgcttcat gtacccgttc tcggcatagg caatggagag ggcgtagaag gggatgccgg
    11821 cgaccgcgtt gtacgtgcca tcgatcgggt ccagaaaaat ggtcccgtgc tcaccctcca
    11881 tggcgacctt ccctgcttcc tctgagatca gggttctgca gaggggatgc cccgcgaggt
    11941 gtgcaaggac gcaatcctcg gcaacgaggt caatatgctt ggtcggggtc ccatcggcac
    12001 ccatccggat attcctgccg gcatccggtg taccgatgag gggatcgatc gcttccgcaa
    12061 catcatgggc aatgtgttca cagacagcca ggaagtccat ggaatcaggg tcctttgggg
    12121 tgtatttatc tcatcatata tagataaaat aagtagcgca tacgcttcag cgagtgtgaa
    12181 tggatgaaag aacagacaga agttggaaaa ttaaaggaag ggcgctatgt cgtcattgat
    12241 gaggagccgt gcaagatcct cggcatttcc gtttcaaaac ccgggaagca tggcgctgcc
    12301 aaggcacgta tcgatgccgt cggcatcttc gatggtgtca agcggtccat cgtccagccg
    12361 gtctctgcaa agacctacgt ccccattgtg gagaggaaga gcggccaggt gatcacgatt
    12421 gccggaacga cggctactct catggacatg aaagagtaca caaacttcga gatcgagatc
    12481 ccctctgata aagcagggca ggtagaggta ggaaaagaga taccctacat cgagtcactc
    12541 ggcaagaaga agctcgatct cgactaagaa cactcacctg aacgatattt ttttcagatc
    12601 ctctcttttc agcacgattt cggcacgatc attcccgctt ctcttcgctc tatcgtgaat
    12661 agagggcggc cgtctcgcca tactggagga cgtgcgatcg catcgcagtg atcagtcccg
    12721 cctttgtcgc accggggggc agggtgagca tggcatcgag cccgtacact ttgagggcgt
    12781 agcgatgggt ggtaccttgt ggaggacagg gtccggaata gccgatcctg ccgaagtcgt
    12841 tcatcccctg gactgcagtg atcgggaacg acacaatacc ttctgggggt atcccggcgg
    12901 ggatctcggg tcctgcagga atattccaga tgagccaggg gcagaaggag cagcaggagg
    12961 gctggaaggg attgactgcc atcacggcga ctgaaacggc attaaggccg gacaacctga
    13021 ttcgcggtga ggtgttggca ccgtcaggta tgggtggggg gaaattccag gaagtcgagc
    13081 ctgacacgga gaggttccat gaacatgata tcgacggtgt tctataaagc aggatctctg
    13141 caccattggt cgtcttgaga agggagtgat aatggccggc ccctcataca tgacaat
//
LOCUS       DAOOCO010000034        13101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4376_length_13101_cov_4.777710, whole genome shotgun sequence.
ACCESSION   DAOOCO010000034 DAOOCO010000000
VERSION     DAOOCO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4376_length_13101_cov_4.777710"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(523..1746)
                     /locus_tag="PK154_03290"
     CDS             complement(523..1746)
                     /locus_tag="PK154_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPW10120.1"
                     /translation="MPPSTLSQDHFSSVKKGYANTIIDRYVSTDRITPDDAGLISEFL
                     AERRAAGGISNGRVDKLTFTLVTWRRFIPPYSTLTIGTVYMGIEAIKNAKTPHGRPYK
                     QNTLFDHVSILKQFLLWMIENEYIILPEKKVKAIKTPRKDTITKTASQLLTPEEVKIL
                     VKASRSSRDRAMIMTLYEGGFRPGEVCQLKWRDMKSDPNGIAVNVNFKTGITRYIRLV
                     MAKKYISEWRADYPLPITPESFVFLNEKRLPLTWAAMAVQINRIAKRAGMTKHITPHL
                     FRHSRITHLLREGASESVVKMMMWGSLNTDMLMTYAHLTGGDVDAEISRLYGLDTKAG
                     QKSARLEPRICPSCNLINPPGEDYCRGCMEALSIQAIADEDAIRRFVIKNPKAFRQFL
                     DRIEEKEGYLSAQKI"
     gene            complement(1810..1894)
                     /locus_tag="PK154_03295"
     tRNA            complement(1810..1894)
                     /locus_tag="PK154_03295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Leu,seq:cag)
     gene            complement(2015..3658)
                     /locus_tag="PK154_03300"
     CDS             complement(2015..3658)
                     /locus_tag="PK154_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW10121.1"
                     /translation="MGDHEETYRTFSLEVPDYYNFGFDVIDRLALEDRNRLAMIWVNQ
                     EGNEKTFTFRQMMNLSNSAANMLLKYGVKKGDRVIIMLPRVPEWWIFAIACIKLGAVF
                     CPCPTMLTPKDLKYRIDAAKISMVITDKENSGKVEEISNDCPTLATRLLTDAKLPGWI
                     SYIEEIDYPAPVSRHLVKIAGMERTKSTDPLVIYFTSGTTGEPKLVLHNHALPLGHVT
                     TGGFWLDLKPHDLHFTYADTGWAKSSWGKFFGPWLQGACILVYDVRGRFKPTELLPVL
                     EKYEITVFCAPPTVYRMLILADLEKFDLTSLRHCVSAGEPLNPEVIRVWKEGTGLTIY
                     EGYGQTELVLVIGTFPCMEARPGSMGKPSPGWQIELHDDDGRPVAPGDTGRIAIRVSP
                     RPVGLFDGYLYDPEATSEVFRGDFYYTGDKAYIDEDGYYWFVGRDDDVIKSSGYRIGP
                     FEVESALLEHPAVKEAAVVGSPDVIRGMIVKAFVVLKEGSAPSEQMAKDIQRFVKKIT
                     APYKYPRSIEFVDDLPKTISGKIRRNVLRERELATHAKY"
     gene            complement(3900..5567)
                     /locus_tag="PK154_03305"
     CDS             complement(3900..5567)
                     /locus_tag="PK154_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW10122.1"
                     /translation="MAERSTGAAAYEELCRTFRIEVPKQYNFGFDVIDAWADKDRNKL
                     AMIWVNQDGTEKKYSFRDLKNLSNQAANILIKYGVQKGDRVLIMLPRIPEWWIFVIAL
                     IKLGAVLCPCPTMLTPKDIKYRLNAGTFRMIITNLENSAKVEEVVTECSTLTCRFLVD
                     GERPGWVSFPRELVYPAPVSFRSVSMPTGKKTLATDPMLIYFTSGTTGEPKMVLHNHS
                     YPLGHLVTATLWQDVGENDVHFTSSDTGWAKCAWGKIFGQWIAGACIFVYDVRGKFKA
                     TELLPLLEKYEITTFCVPPTIYRMLILADLEKFDLRELRRCTSAGEPLNPEVVRVWME
                     GTGRTISEGYGQTETVCCIGTFRGMETRPGSMGRPAPGWHIELHDDEGRPVEQGEEGR
                     IAISIDPRPVGLFVEYVNSPEANAASFVHGFYYTGDKATIDADGYFWFVGRNDDIIKS
                     SGYRIGPFEVESALLEHPAVQESAVVGSPDLIRGVIVKAFIVLKPGYQPSETLIRDIQ
                     SHVRNSTAPYKYPRAVEFVPDLPKTLSGKIRRNELRQRELEKYRSRE"
     gene            5698..6252
                     /locus_tag="PK154_03310"
     CDS             5698..6252
                     /locus_tag="PK154_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HPW10123.1"
                     /translation="MDDKRYDSLKIENIVASGAIADSIDLAEVAQKVANCELNTKRFP
                     GAVFRMEDPKSASLIFSSGKVVLTGIRDKKSLEKGLAIIIQSLKEAGVKTYDSPRVAV
                     TNIVCSYDIGKYINLNRVVITLNLESIEYEPEQFPGLVYRIHDPKIVALLFSSGKIIL
                     TGGKNLEDVKRGLDILEQKLGSIL"
     gene            complement(6254..8944)
                     /locus_tag="PK154_03315"
     CDS             complement(6254..8944)
                     /locus_tag="PK154_03315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW10124.1"
                     /translation="MSPISHLDERILACIRERGFEELSEIQNRAIPLAASGRNLILIA
                     PTGTGKTESAMIPVFDRMLTERTGGFTTLYITPLRSLNRDMLQRLSWWCGELGISVGV
                     RHGDTKNAERRKQALSPPELLITTPETLQALFMGKVLRKHLKNVRHVVIDEIHELAGS
                     KRGSQLSIALERLVAHAGEFQRIGLSATVGNPDEMGRFLCGKRDFSVVEVPVTKRLQI
                     TVECAGDSFERQAEWVSDTIDRFGSTLIFVNTRVTAEALGHQLFSRGDVEVHHGSLSR
                     EIRIDAEERFRSGAIKGLICTSSMELGIDIGHIEHVIQFGSPREVSRLVQRIGRAGHQ
                     LHAVSRGTILALGFDDLLESLVIARRAKAGEVEDVIPPRNPADVLANQVAALAVEYGH
                     IPKEAVTAILERTYPFSSCRELLDEVCAQMAEHRLIRLEGDTICTTMRARRYLAGNLS
                     MIHDEKKVHVFDMVSRRTVATLDESFVVGSIYTGAVFITKGQLWRVLDMDEGRITVEP
                     ARRVKGELPSWEGEQIPVPFGIAREVGALRRSRHFAAYHTDQEVSRFAAGFLSEMDKN
                     RSRVATDRMIVLEDSEEAVICNICAGHKANEALGRVISVLLSARFGTTVGIEIDAYRI
                     LLSLPPSVRASDVREVLAGLEPEHVGGILQLAMKRTTLFKWKLVQIAKKFGAIDPDAD
                     YERLSTYKLTELFENTVVQKEAYRELFSAYMDVDCAAHLLSLVRQGDCAIELARLSIL
                     GAQGLFSSRDQIAPPTPDQAVISTLRRRIDQQEIVLACMNCRKWKSHTVAGRVQEQPV
                     CPRCGARLIAVLKPYEEPLLALASKKEKTSEEKAVEARLIRNANIVLSGRKKAVLALA
                     GKGIGPEHASRILATLTEGDAFYKEILKAERNYIRTRRYW"
     gene            complement(8922..9683)
                     /locus_tag="PK154_03320"
     CDS             complement(8922..9683)
                     /locus_tag="PK154_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW10125.1"
                     /translation="MILEFLSQGPALLVKGRRNVLAVADLHLGIESDLDRHGIHFRSR
                     TRERCERLETCIRETLPDLLLLLGDLKHAIPFPSKQEQREIPEFIAHLRSLVPLGIVP
                     GNHDVAIEQFFEPGELLAKEGVIIDGTGYVHGHMYPDVSLAGMLIIAGHHHPMVSLRD
                     EVGCSLRSPAYLFSRLNDVCIGFPVREGGTESRVLFMPSFNELTGYDVTRTMKEPFSP
                     LSRCISRESSEVLLPDGTFLGPLGALEGYEPDQSP"
     gene            complement(9680..10450)
                     /locus_tag="PK154_03325"
     CDS             complement(9680..10450)
                     /locus_tag="PK154_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10126.1"
                     /translation="MEGAGRIFAGEYSGARYARADLAGESPALITPGGMVCRLLFVAG
                     TLTEKAIPGGETVYARVADPTGVFEIRKIRPDAGLKAALSSIEIPSFVTVLGYARITT
                     NDDEPVPYLDLVDLREVDRTTRDTWIIRTSELTTDRLSRMKEALESGKGSPEVLCAMS
                     QFNTSTASVGMLADMVSRGLEQVAKRSLPEERDAEIRGKILSIIRDSAGKRGVPFDEL
                     VTLAGKAGIPKPMVRDVVRVLLEEDECYQPARDVFKPL"
     gene            10732..>13101
                     /locus_tag="PK154_03330"
     CDS             10732..>13101
                     /locus_tag="PK154_03330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPW10127.1"
                     /translation="MLIVGVIPAFAAGDASASNAAPTLSDVDHEDLIASQPLFFIENK
                     GQISGDVLFEVISNGGLVYFTGDGATIRVIRTINDEFASSTIGFTYEGALKNTGVYGI
                     DPLPCRVNFMRGDPSQWITGVRTYESVAYPDLYPGIDLTYEGKTGAIKSSFLVKPGSD
                     PASIKTVYTGHSGLTVAPNGDLHIQTDVGILTQSVPYCYQEIDGHIVVIPASYLILSD
                     DTVGFGIGDYDEGKDLIIDPEIKYSLYLRGVGFASANGVAADIQGNAYVAGVTFPEVL
                     DPYGSISGGTDAIVVKINPLGTSPEYLNYIGGKGYDAGNAIAVDTNGNAYITGSTDSV
                     DFPVVNAIQSANAGKNDAFVVKLNPDGSSFVYSTYVGGTGNDYGYGITLDSQNNAFIT
                     GSTDSKSFPVNHGSQNTALAGNADAFIVKVNSPGTQFSYSGYYGGTLNDIGYGIDVDG
                     TGAAYITGETFSNNFPLKGAFQSKLGGWSDAFITKVDTAQDAIVYSTYLGGTAKDAGR
                     AIAVDKDSSAHVTGLTESTNFPVKDAFYQSPSSRMRSSFYTRLVPAGNALSLSTYLTG
                     PVADEGRGITIDSFGNVYIAGFTKDTNLKTINAFQPKFGGQSDAFVAKFVPGQKIPEL
                     LSYLGGNGKDEGYAIAVDNNCGVYVVGLTRSTDFPASNPYPTAFLPKEEGGFITALKD
                     GDCCPYPKAGFTFSTNVCDFNVTFTDTSTDATSWAWQFGDGATSSAQNPDHTYLNAGT
                     YTVNLTVQNVCFDGSPRNSTVSKQVVLNPVPATTADFSLDPSSGEAPLNV"
BASE COUNT         2968 a         3618 c         3509 g         3006 t
ORIGIN      
        1 gggatccggc tccaggtagt ctgggtgtgc ctggcagtgt ggattgggac agatcccgtc
       61 ctcggggatg gtgcagacca gcgtggtcca cttcccctcc ttgcggctct tgaagaaagg
      121 acacttcttc gccttcttca cagccgatga cgacgtcgtg ccgaatccca tcagaacacc
      181 accgcaggtg cgcgataaga aggttttttg ctctgcgcaa acaggagaat attggctctt
      241 ccctttgtag tgaagcttgg gaggcaacat tcggggggag ggcccactat tctggacatg
      301 cgtgttcctc gtccgcgatg atgatcaacc cacggtcgac aaaaaattgc ccgataccac
      361 gaaagccggt atggcattca tatgcttcca tagcctctcg tcgtgtgcgt aacacatctc
      421 tttccaaaaa atcacctcta tgcacccatg caaacaagac gagagggagt gcaaaagata
      481 gtgtctgggt ctcatacaca gaaaaaaaag taagagccct tgttaaattt tttgagctga
      541 taagtaccct tctttttcct caatccggtc cagaaattgc cggaatgcct ttgggttctt
      601 tattacaaac cgacggatag cgtcctcgtc agcaatcgct tgaatcgaaa gagcttccat
      661 gcatccccga caataatctt caccgggcgg attaatcaaa ttacacgacg ggcatatccg
      721 tggttccagc cgcgcagact tttgccccgc ttttgtgtcg agaccataga gccgcgagat
      781 ctcggcatcg acatctccgc cagtcagatg agcataggtc atgagcatgt cagtattgag
      841 cgatccccac atcatcatct tgacgacact ctcggaagct ccctccctta gaagatgggt
      901 gatacgggag tgccgaaaca aatggggagt gatatgcttg gtcatccctg ccctcttggc
      961 aatcctgttg atctgaactg ccattgcagc ccaggtgaga ggaagtcgtt tctcattcag
     1021 aaagacaaag gattcagggg tgatagggag ggggtaatct gctctccatt cggaaatata
     1081 tttcttggcc atcacgagcc ggatataccg ggtgatgccg gttttgaaat tcacattgac
     1141 ggcaatccca ttggggtctg atttcatgtc tctccacttg agttggcaga cctcacctgg
     1201 acggaatccc ccttcgtaaa gggtcattat catcgcccgg tcccgggagc ttcgggaggc
     1261 cttgaccaat atcttcactt cctcgggtgt caggagctgc gatgccgtct tggtgatagt
     1321 atcctttctc ggagtcttga tcgccttgac cttcttctcg ggaaggatga tgtactcgtt
     1381 ctcgatcatc cagagcagga actgcttcag gatcgagaca tgatcaaaga gggtgttctg
     1441 tttataaggc cgcccatgag gggtttttgc gttcttgatc gcctcgattc ccatgtacac
     1501 ggtccctatc gtgagcgtgg agtatggcgg gatgaatcga cgccaggtta ccagggtaaa
     1561 ggtgagcttg tcgacacgtc cattgcttat tccccctgct gcccggcgtt cggcaagaaa
     1621 ttccgagatc agtccggcgt catcaggggt tattcggtct gtgctcacat accggtcaat
     1681 gatggtattt gcataccctt tcttgacact ggaaaaatga tcttgtgaaa gagtgctggg
     1741 aggcataaag aattgtttga gagagggata tataaacaaa aaccggggga ttagaacttc
     1801 gtgtccgaat gcgagggatg ggatttgaac ccaagaactc ctgcgagact ggaccctgaa
     1861 tccagcgccg ttgacctggc ttggctaccc tcgcgccttt aactggtttg gcttacgggg
     1921 aaataaaggt gactaaatcc ggtggtgatc tctccctgga aaaaaggagt gacacgaggg
     1981 ggagaggagg ggagcccatc accttcccac tcattcaata ttttgcatgg gttgcaagtt
     2041 cccgctcgcg gagcacgttc ctccggatct tgcccgagat ggtcttgggc aggtcatcga
     2101 cgaactcgat cgaacgggga tacttgtagg gagccgtgat ctttttcaca aaccgctgga
     2161 tatcctttgc catctgctca ctcggagcag atccctcttt taagacaacg aatgctttca
     2221 cgatcatgcc acggatgaca tcaggggacc cgaccaccgc agcctccttg acggcaggat
     2281 gttcgaggag ggcactctcg acctcaaacg gaccgatccg gtacccggaa ctcttgatca
     2341 cgtcgtcgtc tctcccgacg aaccagtaat aaccgtcctc atcgatgtat gccttgtccc
     2401 cggtatagta gaagtcgccc ctgaagacct cgctcgttgc ctcgggatcg tacagatacc
     2461 cgtcaaagag cccgaccggc cgcgggctga cccggatcgc aatcctcccg gtatcccctg
     2521 gcgcaaccgg ccggccgtca tcatcgtgca gttcgatctg ccagccggga gacggcttgc
     2581 ccatcgatcc gggccgtgct tccatgcagg gaaaggtccc gatcaccagc acgagctcgg
     2641 tctggccgta tccctcgtag atcgtcaggc cggtcccttc cttccagacc cggatcacct
     2701 caggattgag gggttcgccc gcactcacgc agtgccggag ggaggtgaga tcgaacttct
     2761 caagatcagc gaggatcagc atccggtata ccgtgggagg agcacaaaag acggtgatct
     2821 cgtacttctc aaggaccgga aggagctcgg tcggcttgaa cctcccccgg acatcgtaca
     2881 cgaggatgca ggcaccctgt agccagggcc cgaagaactt cccccaggac gacttggccc
     2941 agccggtatc ggcataggtg aagtggagat cgtgtggttt caggtcaagc cagaatcctc
     3001 ccgtcgtgac atggccgagg gggagggcat ggttgtgaag gaccagtttc ggctcgccgg
     3061 ttgttcccga ggtgaaataa atgacgagcg ggtcagtgct cttcgtccgc tccatcccgg
     3121 ctatcttcac caggtggcgg gagaccggag ccggataatc gatctcctcg atatagctga
     3181 tccacccggg gagttttgca tcggtcagaa ggcgggtggc aagcgtgggg cagtcattgc
     3241 ttatctcctc caccttgccg gaattctcct tgtcggtgat caccatggag atcttggccg
     3301 catcgatccg gtacttcagg tcctttggag tgagcatcgt ggggcagggg cagaaaaccg
     3361 ctcccagctt gatacaggcg atggcaaaga tccaccattc cggaacacgg gggagcatga
     3421 tgatgacacg gtcccccttc ttcaccccgt acttcaggag catgtttgcc gccgagttcg
     3481 agaggttcat catctgccgg aaggtgaacg tcttttcgtt cccctcctgg ttcacccaga
     3541 tcatggcaag ccggttccga tcctcgaggg caagccgatc gatcacatcg aacccaaaat
     3601 tgtaatagtc aggtacctcc agtgagaatg ttcggtaggt ctcctcgtga tcacccatag
     3661 cgtgtccttc acagggcata tgaacagaat aatatcatct gggaaatctt taacctgtct
     3721 ttttctgcat cagcaggaga gaacaaaaga agggattgtc tgctggatga gggcccgttc
     3781 ctgaaccgtt ccctctgtca caggaatacc ttggcttgaa aggtggaacc ggcagggaga
     3841 agcggccaga tgaagcaatc caggaaggca gcgtgccagg ccgggacggg gaagagggat
     3901 tattccctgc tccggtactt ctccagttcg cgctgccgga gctcgtttcg gcgtatcttc
     3961 ccggacaggg tcttggggag atcagggaca aactcgaccg cccggggata cttgtaagga
     4021 gcggtggagt tccgcacatg cgactggata tcacggatca gggtttccga tggctgatac
     4081 cctggtttga gaacgatgaa cgctttgacg atgactcccc ggataagatc gggagacccg
     4141 accactgccg attcctggac tgccggatgc tcgagaaggg cactctccac ctcaaaagga
     4201 ccgatccggt atcccgagct cttgatgata tcatcattcc tcccgacgaa ccagaagtag
     4261 ccgtcggcat caatggtagc cttatccccc gtataataga atccgtgtac aaacgaggca
     4321 gcattcgctt cagggctgtt tacatactcc acgaagaggc cgaccgggcg gggatcgata
     4381 ctgatagcga tccgtccttc ctctccctgc tcgaccggcc gcccttcgtc atcatggagc
     4441 tcgatatgcc atcccggcgc cggtctcccc attgacccgg gtcgtgtctc cataccccgg
     4501 aatgtgccga tacagcagac cgtctcggtc tggccatacc cctcggagat ggtccggccg
     4561 gtcccctcca tccataccct gaccacttcc ggattgagcg gctcgccggc actggtacag
     4621 cggcggagtt cgcgcaggtc aaacttctcg aggtctgcga ggatcagcat ccggtatatg
     4681 gtagggggga cacagaacgt ggtgatctca tacttctcaa gcagcggaag gagttcggtc
     4741 gccttgaact tgcccctcac atcatagaca aagatacagg ccccggcgat ccactggccg
     4801 aagatcttgc cccaggcgca tttggcccat ccggtatccg atgaggtgaa atgcacatcg
     4861 ttctcaccga catcctgcca taatgtcgcc gtgacgaggt gcccgagcgg gtagctgtgg
     4921 ttgtggagga ccatcttcgg ttctcccgtg gtacccgagg tgaagtagat cagcatcggg
     4981 tcagtcgcaa gcgtcttttt tccagtcggc atactgaccg aacggaatga caccggggca
     5041 ggatagacca gctccctggg gaagctgacc caccctggcc gttcaccgtc gacaaggaac
     5101 cggcatgtca gcgtcgaaca ttcggtcacc acctcctcaa ccttggccga gttctcgagg
     5161 ttggtgatga tcatccggaa cgtcccagca ttcaaccggt acttgatgtc ctttggcgtc
     5221 agcatggtgg gacagggaca gagcaccgca ccgagtttga tgagggcgat cacaaagatc
     5281 caccactcag ggatccgggg gagcatgatc agcacgcgat cgcctttctg gacaccatac
     5341 ttgatcagga tattggccgc ctggttggag aggtttttca aatccctgaa ggaatacttc
     5401 ttttcggttc catcctggtt cacccagatc atggcaagct tgttccgatc cttgtcagcc
     5461 catgcatcga tcacatcaaa tccaaaatta tactgtttcg gcacctcgat ccggaacgtc
     5521 ctgcagagtt cctcatacgc cgctgcacca gtgcttcttt cagccataaa acgtcaaaaa
     5581 tgttggagat gaaaggagat gaatctgcct tttcttattc gctcacgaaa tgaccaaaca
     5641 tcctctttcg gggtaatcca gtagtattat agtggtctac actgcatgta ctgatcaatg
     5701 gacgataaga gatacgattc cctgaaaata gagaatatcg tcgcatcagg tgccattgcc
     5761 gattccatcg atctcgcaga agttgcacaa aaggtggcga actgcgagct caacaccaag
     5821 agattccccg gcgctgtctt ccgcatggag gatccgaaaa gcgcatcgct catcttctca
     5881 tcgggcaaag tggtgctgac cgggatcagg gacaagaaat ctcttgaaaa aggtctggcc
     5941 attatcatcc aatcactcaa ggaggcaggt gtcaagacct atgacagccc ccgcgttgct
     6001 gtcacgaata tcgtttgttc gtacgacatc gggaagtaca tcaacctgaa cagggtggtc
     6061 atcaccctta acctcgagag catcgagtat gagccggaac agttcccggg gcttgtgtac
     6121 cggatccacg atccgaagat cgttgccctg ctcttctcat caggaaagat catcctgaca
     6181 ggcggtaaga atctcgagga tgtcaaacgg ggccttgata tcctcgaaca gaagctcgga
     6241 agtatcctct gatctaccag tatctccggg tccttatata gttccgttcg gccttcagga
     6301 tctccttgta gaaggcatcc ccttccgtga gggtggcgag gatccgggag gcgtgttcgg
     6361 gacctatccc ctttccggcc agggccagta ccgccttttt tctcccggaa aggacgatgt
     6421 tggcatttcg tatcagcctt gcttcgaccg ctttctcctc gctcgtcttc tccttctttg
     6481 acgcaagggc gagcagcggt tcctcgtatg gtttcaggac ggcgatcagc cgggccccgc
     6541 atcttggaca gaccggctgt tcctggaccc tccctgcaac cgtgtggctc ttccacttcc
     6601 ggcagttcat gcaggcgagc acgatctcct gctgatcgat tcggcggcgg agcgtactga
     6661 tcacggcctg gtcaggcgta gggggggcga tctgatctct cgaagagaaa agtccctggg
     6721 cacccaggat gctcagccgt gcgagttcga tggcacaatc cccctgcctt acgagcgaca
     6781 ggaggtgagc tgcgcaatcc acgtccatgt atgcagagaa gagttcacgg tatgcctctt
     6841 tctggacaac ggtattctcg aacagttcgg tgagcttgta ggtgctgagc cgttcataat
     6901 ccgcatcggg atcgatagct ccgaattttt ttgcgatctg gacaagtttc cacttaaaaa
     6961 gagtggtcct cttcatggcg agctggagga tccctccgac gtgttccggc tcgagtccgg
     7021 cgaggacctc cctgacgtcg gatgccctga ctgaaggggg cagggagagc agaatccggt
     7081 atgcatcgat ctcgatccca accgtagtac cgaaccgggc tgagaggagg accgagatca
     7141 cccgccccag cgcttcattt gccttatggc ctgcgcagat attgcagatc accgcttctt
     7201 cggaatcctc gagaacgatc atccggtcgg ttgcaacacg gctccggttc ttatccattt
     7261 cagaaaggaa cccggcagca aaacgggaca cctcctgatc agtatgatat gcggcaaaat
     7321 gccggcttcg ccggagcgcc cccacctcgc gtgcaatccc gaacggaaca gggatctgct
     7381 caccttccca agagggcagc tctcccttga cccgtcgtgc cggctcgacc gtgatccgcc
     7441 cttcatccat gtcaaggacc ctccagagct gccctttcgt gatgaagacc gcaccagtgt
     7501 agatcgatcc caccacaaac gattcgtcaa gcgtagcgac ggtcctcctt gagaccatat
     7561 cgaagacatg aaccttcttc tcatcatgga tcatcgacag gtttccggca aggtagcgac
     7621 gtgctctcat ggtcgtgcag atggtatctc cctcaagccg tatcagccgg tgttctgcca
     7681 tctgggcaca tacctcatcg aggagttccc ggcagctgct gaagggatat gtcctttcaa
     7741 ggatggccgt gaccgcttct ttcgggatat gcccgtattc aacagccagg gctgcaacct
     7801 ggtttgcaag gacatcggcc gggttcctgg gagggatgac gtcttcaacc tcgccggcct
     7861 ttgcccgcct tgcaatgacc agggactcga gcagatcatc gaatcccagg gcaaggatgg
     7921 tacccctgga tacggcatgc agctgatgcc cggcccggcc gattctctgg accaggcggg
     7981 agacctccct tggtgagcca aactggataa catgctcgat atgcccgata tcgatcccga
     8041 gttccatgga tgacgtgcag atcagccctt ttattgcgcc cgacctgaag cgttcctcgg
     8101 catcgatcct gatctcccgc gagagcgatc catggtgcac ctcaacatcc ccccgggaga
     8161 agagctggtg gccaagggct tcggcagtca ctcttgtatt gacaaagatc agggtcgaac
     8221 cgaaccggtc gatggtatca gagacccact ccgcctggcg ctcaaagctg tcacccgcac
     8281 actcaaccgt gatctggaga cgcttggtga cagggacttc caccaccgaa aaatccctct
     8341 tcccgcagag gaaccgtccc atctcatcgg gattcccgac ggtagccgag agcccgatcc
     8401 tctggaattc gcccgcgtgg gcgacaagcc gttccagggc aatggagagc tgtgatcccc
     8461 ttttgctccc tgccagttcg tggatctcgt caatcaccac gtgcctgaca ttcttcaggt
     8521 gcttccggag cacctttccc atgaacagtg cctgcagcgt ttccggggtg gtgatgagga
     8581 gttccggggg ggataatgcc tgttttctcc gttcggcatt ctttgtgtcc ccatgcctga
     8641 ccccaacgga tatcccgagt tcgccgcacc accaggagag ccgctggagc atatcgcggt
     8701 tcagggaacg gagcggcgtg atgtagaggg tcgtaaaccc tccggttctc tccgtaagca
     8761 tccggtcaaa gaccgggatc atggcgctct cggtctttcc cgtcccggta ggcgcaatca
     8821 ggatgaggtt cctgccggac gctgcaagag gtattgcacg attctggatc tcggagagtt
     8881 cctcaaagcc ccgctcccgg atgcaggcta aaatccgctc gtcaaggtga ctgatcgggc
     8941 tcatatccct ccagtgcccc aagaggtccg agaaaggtgc catcggggag gagaacctcg
     9001 gatgattccc ggctgataca ccgggagagc gggctgaacg gttctttcat tgtcctcgta
     9061 acatcgtacc cggtcagttc attgaacgag ggcatgaaaa ggacccggct ctcagttccc
     9121 ccttcccgga caggaaatcc aatgcaaaca tcattgagac gtgaaaagag gtaggcggga
     9181 gagcggagag agcatcccac ttcatccctg agagagacca ttgggtggtg atggccggca
     9241 atgatcagca ttcctgcaag ggacacatca ggatacatat ggccatgtac atatcccgtg
     9301 ccatcgatga tcaccccctc ctttgccagc agctcccccg gctcaaagaa ctgctcaatg
     9361 gcgacatcat gattcccggg gacaatcccg agaggaacaa gggaacggag atgtgcgata
     9421 aattcaggga tctcgcgctg ttcctgcttt gaggggaaag ggatggcatg tttcaggtca
     9481 ccgagcagga ggaggagatc ggggagggtc tcccttatgc aggtctcaag gcgttcgcac
     9541 cgctcccgtg ttctgctcct gaaatggata ccatgcctgt cgagatccga ttcgatgcca
     9601 aggtggaggt cggcaacggc aagaacattc ctccttcctt tgacaaggag tgctggtccc
     9661 tggctcagaa attcgagtat catagaggct tgaaaacgtc ccgggcaggc tggtaacatt
     9721 cgtcttcttc gagcaggaca cggacaacat ctctcaccat cggcttcggg atcccggctt
     9781 ttccagcaag ggtgaccagc tcgtcaaacg gcactcctct cttccctgca gaatccctga
     9841 taatcgagag gatttttccc cttatttcag catctctttc ctcgggcagc gatcgtttag
     9901 caacctgttc cagtcccctg gataccatgt ccgcgagcat cccgactgat gcagtactcg
     9961 tattgaattg tgacatcgca caaaggactt cggggcttcc tttccctgat tcgagtgctt
    10021 ccttcattcg ggagagccgg tcggtggtga gttcggaggt cctgataatc caggtatccc
    10081 tggtggttcg atcaacctcc ctcagatcta ccagatcaag gtaaggaacc ggctcatcat
    10141 cattggttgt gatccgggca tacccaagca cggtcacaaa ggagggtatc tcaattgatg
    10201 agagcgctgc tttcaggcct gcatcaggcc ggattttccg gatctcaaaa acaccggtcg
    10261 gatcagccac ccgtgcatag accgtctcac cgccgggtat cgctttctca gtcagggttc
    10321 ctgccacaaa gagcaggcgg cataccatgc cgccgggggt gataagagca ggagattccc
    10381 ccgccaaatc ggcgcgggca tatcgtgccc ctgaatattc tcctgcaaag atccgcccgg
    10441 ccccttccat ttttttcctg tatccttctc agaatagtga gatcccgttc gccgtgatat
    10501 ctccgtaaat caatgctgtt gtgatagtat actgagccct gggacaacat atccattatg
    10561 atgaaggaaa taacagagtg cagaaaagaa tgtcagctta aatcgctgct ggggaaaaaa
    10621 gcagtcaaaa tagcgctttt tataggaaag ttttatatca tatgaattct tcatctgaat
    10681 ttaagggttt ttatgagaca aaaagagatc ccgctgttat tcgtactttt ggtgctcata
    10741 gtaggagtca tccctgcctt tgccgcaggg gatgcttctg catccaacgc tgcaccaacg
    10801 ttgtctgatg ttgaccatga ggacctgatc gcctcacaac cgctgttttt catcgaaaat
    10861 aaaggtcaga taagcggaga tgtcctcttt gaggtcatct cgaatggggg gcttgtctac
    10921 tttaccggtg atggagcgac catacgggta atccggacga ttaacgatga gtttgcctcc
    10981 tcaacgatcg gatttaccta cgagggggct ttgaaaaata ccggtgttta tggaattgac
    11041 ccccttccct gcagggtaaa tttcatgaga ggcgatccct cacaatggat tactggtgtc
    11101 cggacgtacg agtcagttgc ctatccggac ctgtatccgg gtatcgatct cacctatgaa
    11161 gggaaaaccg gtgccataaa gagcagtttc ctggtaaagc cgggatcgga ccctgcctca
    11221 attaaaacgg tgtataccgg ccattcaggg ctgacggtgg caccgaatgg tgatctccac
    11281 atacagaccg atgtaggaat actcacccag agtgtaccct actgctacca ggagattgat
    11341 ggacatatcg tggtaattcc tgcctcctac cttatcctgt ccgatgatac cgtgggattc
    11401 ggaatcggag attatgatga gggaaaagac ctgatcatcg atcccgagat caagtatagt
    11461 ctttacctga gaggggttgg atttgcgagt gcaaacgggg ttgcagcgga tatccagggg
    11521 aatgcgtatg ttgccggagt taccttccct gaagtgcttg atccctacgg atccatcagt
    11581 ggaggcaccg atgcaattgt tgtcaaaatc aatcctctgg gaacctcacc agaatacctc
    11641 aattacatcg ggggtaaagg gtacgatgcc ggaaacgcca tagcggttga cacgaatggc
    11701 aatgcatata ttaccgggtc gaccgattca gtggatttcc cggttgtcaa tgccatccag
    11761 tcagcgaacg ccggcaagaa tgacgccttt gtcgtgaagc tgaaccctga cgggagctcg
    11821 tttgtctact ccacgtatgt cgggggaacc gggaacgatt acggatacgg tatcactctt
    11881 gattcgcaaa ataatgcctt tataacaggt agtacggatt cgaagtcatt cccggtcaat
    11941 cacggatccc agaatactgc actggcagga aacgctgacg cgtttattgt gaaggtaaac
    12001 agccccggaa cccaattttc gtactccggt tattatgggg ggaccctgaa tgatatcggc
    12061 tatgggatcg atgttgacgg aacgggagct gcgtacatta ccggtgaaac gttttcgaat
    12121 aatttcccgc tgaagggtgc attccagtca aaactgggcg gctggagtga tgcattcatc
    12181 acgaaggtgg atacagcaca agacgccatt gtctattcca cctacctggg aggaaccgca
    12241 aaagacgccg gccgtgccat tgccgttgac aaagattcct ctgcccatgt gacagggttg
    12301 acagagtcca caaatttccc ggtaaaggat gcattctatc agtcacccag ctcccgcatg
    12361 cggagttcgt tctatacccg tctcgttcct gcgggcaatg cactctcttt gtccacctac
    12421 ctgactggtc ccgtagctga tgagggcaga ggaatcacca tcgattcttt cggcaatgtc
    12481 tatatagcgg gattcaccaa ggataccaac ctcaagacaa taaatgcatt ccaaccgaag
    12541 ttcgggggac aatctgatgc ttttgttgcc aaattcgtcc cgggacagaa gataccggaa
    12601 ctcctcagtt atcttggcgg gaacggcaag gacgagggat atgcgattgc ggtcgataat
    12661 aactgtggag tatacgtcgt aggactcacg aggtccaccg atttcccggc aagcaatcca
    12721 tacccgacag cattcctgcc aaaggaagag ggaggattta tcaccgcact gaaagacggg
    12781 gactgctgtc cttatccgaa agccggtttc acgttctcca ccaatgtctg tgacttcaat
    12841 gtcaccttca ccgatacctc gaccgatgcc accagctggg catggcagtt cggcgacggt
    12901 gccacttcca gtgcacagaa tccggatcat acgtacctga atgcaggcac ctacactgtc
    12961 aacctgacag tacagaatgt ctgtttcgat gggagcccac ggaactctac ggtttcaaag
    13021 caggttgtac tgaatcctgt accggcgacg accgctgact tcagtctgga tccctcatcc
    13081 ggtgaagcac ccctcaatgt c
//
LOCUS       DAOOCO010000035        12840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4508_length_12840_cov_4.723191, whole genome shotgun sequence.
ACCESSION   DAOOCO010000035 DAOOCO010000000
VERSION     DAOOCO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4508_length_12840_cov_4.723191"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK154_03335"
     CDS             <1..296
                     /locus_tag="PK154_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10128.1"
                     /translation="TTLSANLTSGSDSGNATAIKANLTSKSNITNTTPTITTQVTTVT
                     TTTPPTTVPPAQPFWSTFPMAWLIPVIVVIILLGALAYYNYRKGQEGELFEEK"
     gene            686..1660
                     /locus_tag="PK154_03340"
     CDS             686..1660
                     /locus_tag="PK154_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HPW10129.1"
                     /translation="MDESYTIWIEKYRPATLAEIVGQDEIVERLQSYVRAGSLPHLLF
                     TGSAGVGKTTAAVALAKEFFGEAWNLNFREMNASDERGIDVVRNQIKQFARTSPLGGA
                     EFKILFLDEADALTPDAQAALRRTMESYAQTCRFILSCNYSSKIIDPIQSRCAIYRFK
                     PIDRSAITAEINRIADQEHLAVSEDAIEAIAYISQGDMRKAINALQGAAILSPEITKD
                     RIYAITSTARPDEIEELLSTSLSGEFEAAEALLSQLLHERGIAPNELINQCYRALIRK
                     EMDDSLRVALIDQLGITDFRLSEGASSDLQMDAMIAQFVMHAKRSRTG"
     gene            1665..3779
                     /locus_tag="PK154_03345"
     CDS             1665..3779
                     /locus_tag="PK154_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HPW10130.1"
                     /translation="MEQEPAAEIIDRDADWNRFLKSRYKKQLSEISREYPYKKSLFID
                     YRELESFGKTGIRMADELLDNPGKVIEDVRSSVLAHQLIKGKDGKGTRDINVRFTSLP
                     KKVAIRQIRSDHINKFISVEGILRKTTEVRPRIVEAFFRCPGGHITIREQGYGKFREP
                     DGCATDGCTFKKLDLVPKRSKFVNSQKLRIQESPEGLRGGEQPQTLDVDATDDLTGLV
                     APGDRVVMNGILRSIQRVSHGEKSTIFDIYLECNSIEIAEKEFEEVEIDEKAEEEILA
                     LSHDPSIYQKITHSIAPTIYGNEEVKEAISLQIFGGITKEMPDGSHLRGDIHVLLIGD
                     PGIAKSQLLRYVVKLSPRAIYTSGQSSTSAGLTATAVKDEFGDGRWTLEAGALVLADM
                     GIAAIDEMDKMQKEDRSALHEAMEQQSISVAKAGITATLKSRCALLGAANPKYGRFDD
                     FAPIGEQINMPASLLSRFDLIFIMGDKPDEKKDGAIAEHILKAHSIGETIVQHRKAPI
                     PGVNEEYIAEQLKPVTPEIEPGLFRKYVAYAKRTCFPRLSDTAREALIAYYMRLRGLA
                     SESNKPVPVTARQLEALVRLAEASARIRLSRDIEATDAERVITIVDKCLRQVAYDPKT
                     GSFDIDKVVTGYSKGKRDLIRRIKEVIRMVADESGRAKVDEVIEQIAQEGFEKDEIRK
                     QIEMFLRQGEAMEPKPGIIKLI"
     gene            3818..4156
                     /locus_tag="PK154_03350"
     CDS             3818..4156
                     /locus_tag="PK154_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HPW10131.1"
                     /translation="MASEHDQAIFEAAIKLGALYHQWVGTPVSPSTASLLESAIQESV
                     SLQPYVADIHVSLDRSQMAENIFGYSELRGTMLTVDIITKVGSSCCHARLQRRGDYPL
                     MEIVSCHEEP"
     gene            4143..4970
                     /locus_tag="PK154_03355"
     CDS             4143..4970
                     /locus_tag="PK154_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW10132.1"
                     /translation="MKSHKFPITDDHIHIDPVNGRGLEAAKDFLRAGGTHLFLVTKPS
                     SSFGVLPHTGDEFSLVYEKTIAIADQIRALGITVFVILGVHPAEMTRLSATTPLSETV
                     AIMKRGIDIAASYIGEGRAVALKSGRPHYPVPDEVFSASNEVLSHALTLAADLKCAVQ
                     IHAETGPCSDVVGMAEQAGLPSHKVVKHYATPDTPLTPSLLASHPDIPQLARLRRPFT
                     METDYMDENTRPGAVIGPKSVPRITRKLINEGLISEDDAFRIHKETPERVYGVEIIL"
     gene            5026..5319
                     /locus_tag="PK154_03360"
     CDS             5026..5319
                     /locus_tag="PK154_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HPW10133.1"
                     /translation="MYLKVHRTPAGDEVVAVCDRELLDTCIQQGDLEIHISGDFYGRS
                     PASPDEVRAALRGAGNCNLFGERTVALAIEMGLIDATGFIRIGSVPHAQIIRL"
     gene            5297..6367
                     /locus_tag="PK154_03365"
     CDS             5297..6367
                     /locus_tag="PK154_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HPW10134.1"
                     /translation="MPRSSGSEKKIRENICPRCGAASATGELCGSCRIHENPWFTCGK
                     RVVCILCPTCGSRKEGGVWTDTVAEREEIARDLVQRSVQIVPEVARPVLKISVKEMTI
                     NRSLAEVVLEGTLFGEPVHAMCQVEILWQKEQCDRCSRISGSYYEGIVQVRASGRRPY
                     RHEIRTVERIAREIEEGCYASGERLSFISDTDETKDGLDITVGSQHIGQAIATAIVQR
                     LGGRFSTHPKLVGERAGKRLYRITYSVRLPRYGKGDVIVVNGRPGEVIYASGREIRYR
                     EIPNGIERSAREDRVERYIGSTRDAREYLVTFRQGNTIGVLDPETGIPRECFVGRDAE
                     YVPGQTIRILNDGDDLLVVGER"
     gene            6364..7230
                     /locus_tag="PK154_03370"
     CDS             6364..7230
                     /locus_tag="PK154_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPW10135.1"
                     /translation="MRVREVPARDLPGIAEAEWRMRERRPFVSNGIAYVPVRDGYPAD
                     RELAPKARYDGRGFYMIGTIAVLHGREPTSAEIREIEAWKKPTGILWIRSYQGRERIP
                     DVRLISGSSGEICHHELGMVFWLDPAQVMFSSGNRAEKQRMMRLIRPGERVADMFAGI
                     GYFSIPAGMAGATVHAMEIHPVAYRYLLRNIDENHLENRIFAGLGDCRELLSGTYDRI
                     IMGHFDSSLMLDAAFDHIRTGGTIHMHTAGRNPPFIPEEYRDACRDAGTVRRIKKVAP
                     HTWHYVLDMRAG"
     gene            7230..8387
                     /gene="coaBC"
                     /locus_tag="PK154_03375"
     CDS             7230..8387
                     /gene="coaBC"
                     /locus_tag="PK154_03375"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPW10136.1"
                     /translation="MEPGSLFKKRIILGVTGSIAAVETVRLAHALRREGASVTAVMSE
                     AATRIIHPDALTYATGNDAITALSGHVEHVTFCGDGGCADLLLIAPCTANTLCKIARG
                     IDDTPVTTFASTALGAGLPVVVAPAMHESMYRHPGVSECIRCLSSWKVTVIPPRREEG
                     KAKIADIDEIVLWCCRALPGSPLAGKHVLITSGPCREPVDDIRVLTTRSTGRMGRELA
                     LQAFRLGAEVTVVHAGVIPCVRNIPIETAGEMHDAVHRICAERIPDIYLSAAAISDYA
                     PEPYRGKLRSGQTLSIPLHPLPKLIDEVCRRYNTSVIAFKVGEEGEEGAAPLIAKGAD
                     LVVINGPESMGSDTTDAIISGAGGREQIRGTKRELAARIWERIGTLLLPQG"
     gene            8419..9315
                     /locus_tag="PK154_03380"
     CDS             8419..9315
                     /locus_tag="PK154_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPW10137.1"
                     /translation="MRRSGRSPQEPVAASVTASCPGHISGYFRPVTGSGYQTTGSIGA
                     GIVITEGVTVRVTPADTTSITVRRLSSEGAVRELIHDAPPLAYVAGRLGVFAHIETEC
                     TLPIGAGFGLSAAALMASVSALNELFHLGMTKKACSELAHESEIVHRTGLGDVAACQG
                     GGRDFRRGPGTGAEIRRFYDLHEPLYAVNFGPLPSPLVLGSPDALTRISAAYPEEYPG
                     SPELFFRLSYRFARGSGLLTQELSDLLVECSRENIPASMTMLGNGIFAMGKRAADLLG
                     SYGEILELHVAPRGVEILEEFS"
     gene            9312..10043
                     /locus_tag="PK154_03385"
     CDS             9312..10043
                     /locus_tag="PK154_03385"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HPW10138.1"
                     /translation="MIPEDHPRYRSLQVREHLARCAAEGILSLEGLTAHGRGEAFDYL
                     IGEKTMESALRAERAAAALLLCAERPVISVNGNTAALAAGSIALLQQASNAAVEVNLF
                     HRTEERIRKITTLLERHGVTVLEGIPERLLPLSHARALCLPGGIGSADVVLVPLEDGD
                     RCQALVDAGKTVITIDLNPLSRTAMTATLTIVDEVTRAIPQVTRFCRALTREEVQLCA
                     TGYENRELLRSAIREIQLRLDHALD"
     gene            10030..11028
                     /locus_tag="PK154_03390"
     CDS             10030..11028
                     /locus_tag="PK154_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW10139.1"
                     /translation="MLWIEKYRPKSFEEIRGQDHVVSHLKRAARSGDIPHLLLTGPPG
                     TGKSVAVECLAYQLYGERMAENMTVIQTADLFEQGKKYLEGDERYGHLYQREQSLLSN
                     FKNIVRWYASIRPLDSDFRIIVFEGASAMTREAQQGIRRIMERYSRTCRFIFVTSRPS
                     ALIPAISSRCLPFFFVPVESRVVGDHLHAILASEFGDTSPLSDDDTDLIVQAAAGDMR
                     KAVVLLQVSTGEECLGNLIRCSQTETGQIAQAALTAVSTSDMISAVRRFETLMIEYGL
                     TSREVLHEFRTAVRREYNDPRLSIAIADTDYTLSHGNNEYLQLNAMLARIIAEVFS"
     gene            complement(11044..12384)
                     /locus_tag="PK154_03395"
     CDS             complement(11044..12384)
                     /locus_tag="PK154_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPW10140.1"
                     /translation="MDVEEYVRRGIRQGIFEKDLRAALANQIRVFKPEEDQEYAERFA
                     QAVINEVKNSTGIGGDFFSYHHSGVSMGEFGVGSRGSGDFFAHRQIARVIGRTRAAVG
                     VDEMDDGGVVEVAGTAGGRYIVCAVDGMHSRLSDFPFLAGFHATRATLRDAYVMGARP
                     ILLFSDIHVADDGDVAKIFEYTAGITTVGEMMGVPLVSGSTLRIGGDMVLGERMTGCV
                     GTVGIARHLTPRKSAAPGDVILMTEGAGGGTIATAAIYSGFPEVVERTINLQFLKAVE
                     ALLGSPVIGEVHAMTDVTNGGLRGDAHELAQTAGCRVVIVEDRVRGIADAKVQEMLDA
                     LAIDYLGVSLDSLLIVAPGDAVPALREVISDAGVRIEEIGRVEQGSPGAYLVSDGKEQ
                     DFSPRFRESAYTPVKKVADRHPADLAGMKMAVEKAADMAIEKKRRIIRYLSSVN"
BASE COUNT         2948 a         3627 c         3704 g         2561 t
ORIGIN      
        1 cgacaaccct ctcggcaaac ctgacgtcgg gcagtgacag tggaaatgcc actgcaataa
       61 aggcgaatct cacatcaaag agcaatatca ccaacaccac tcccaccata accacccagg
      121 tcaccacggt gacgaccacc acgcccccca ccaccgtgcc accagcacag ccgttctggt
      181 caaccttccc catggcatgg ctcatcccgg tgatcgtggt aatcatcctt cttggagccc
      241 tcgcatacta caattaccgg aagggacagg aaggagagct gtttgaagag aaataatcct
      301 cttttttaaa aatcagggat acgggcattg tatccacctt tttttcatca acggatttca
      361 tatcttccac agccctcacg ggatgactct cgaggctact cccgccatgt ggtgtcccct
      421 aagatgtgag agaagattat caaccacctc ctccctcctt ccagagttcc cgcaagggat
      481 ttttcacttt cgtcctgtgt gcctatagtt caaaggtgac gcaggtgatt ttttcattga
      541 tgcatgaaag aggaactatg ttcagaccct gctgcccgac gaaaaaacca gaggccttgg
      601 caggaagatt ggacagcgaa aaataaagag atcaagacta atattcagaa cgaatataga
      661 agataaacac gatactggag cagttatgga cgagagctac acgatctgga tcgaaaaata
      721 ccggccggca accctcgccg agatagtggg gcaggacgag atcgtggaac gcctccagtc
      781 gtatgtgagg gcagggagcc tcccccacct cctcttcaca gggagtgcgg gggtgggcaa
      841 gaccaccgcg gccgttgctt tggcgaagga attcttcggg gaggcatgga acctgaactt
      901 cagggagatg aacgcgtccg atgagcgcgg tatcgatgtg gtgagaaacc agatcaagca
      961 gttcgcacgg acatccccgc tgggaggggc ggagttcaag atcctcttcc ttgacgaagc
     1021 ggatgcactc acccctgatg cccaggctgc cctccgccgg acgatggaga gctatgcaca
     1081 gacctgcagg ttcatcctct cctgcaatta ctcttccaag attatcgatc ctatccagag
     1141 caggtgtgcc atctaccggt tcaaacccat tgacaggtcc gcgataaccg ctgagataaa
     1201 caggattgca gatcaggaac accttgccgt gagtgaggat gccatcgagg cgatagccta
     1261 catctcgcag ggtgacatgc gaaaggcgat caatgccctg cagggtgcag ccatcctttc
     1321 accggagatc acgaaggaca ggatctatgc aatcacatcc accgccagac cggatgagat
     1381 cgaggaattg ctctcgactt ctctttctgg cgaatttgaa gccgccgaag cactcctctc
     1441 acagctgctc cacgagcggg gcattgctcc caacgagctc atcaaccagt gttaccgggc
     1501 tctaatcaga aaagaaatgg atgacagcct ccgcgttgca ctcatcgatc agctcgggat
     1561 caccgatttc aggctctctg aaggggcaag cagcgacctg cagatggatg cgatgatcgc
     1621 acagttcgtg atgcatgcaa aacgatcacg caccgggtga caggatggag caggagccgg
     1681 ccgccgagat cattgatcgc gatgctgact ggaacaggtt cttaaagagc cgttacaaaa
     1741 agcagctgtc agagatatcc cgcgaatatc cctacaaaaa atctcttttt atcgattacc
     1801 gggagctcga gagtttcggg aagaccggga tcaggatggc ggatgagctc cttgataacc
     1861 cgggcaaggt gattgaggat gtgcgatcat cggtcctcgc ccaccagctg atcaagggga
     1921 aggatgggaa agggacccgg gacatcaacg tcaggttcac cagccttccc aaaaaagttg
     1981 ccatccggca gatccggtca gaccatatca acaaattcat cagcgtggag gggatcctcc
     2041 ggaagacgac ggaagtacgc cccaggatcg ttgaagcctt ttttcggtgc ccgggtggcc
     2101 atatcacgat ccgggagcag gggtacggta agttcagaga gcctgatggc tgtgcaactg
     2161 acggatgcac cttcaagaaa ctggaccttg tgccgaagcg ctcgaaattc gtgaactcgc
     2221 agaaactccg gatccaggag tccccggaag gtctccgggg aggggaacag ccgcagacgc
     2281 ttgatgtcga tgcaactgac gatctgacag ggctcgttgc gccgggtgac agggtcgtca
     2341 tgaacggaat ccttcgatcg atacagcggg tcagccatgg ggagaagagc accatatttg
     2401 acatctacct cgaatgcaac tcgatcgaaa ttgcagaaaa agagtttgag gaggtcgaga
     2461 tcgatgagaa agctgaggaa gagatcctcg ccctgtcgca cgatccctct atctaccaga
     2521 agatcaccca ttcgatagcc ccgaccatct atggaaacga ggaggtgaag gaggcgatat
     2581 ccctccagat ctttggcggg atcaccaagg agatgcctga cgggagccac ctccggggtg
     2641 atatccatgt cctcctgatc ggtgacccgg gtatcgcaaa aagccagctg ctcaggtacg
     2701 tggtcaagct ctctccccgg gccatctaca ccagcggcca gtcatccaca tcggcaggcc
     2761 tgaccgcaac cgcagtcaag gacgagttcg gggacgggcg ctggaccctt gaggcaggag
     2821 ccctggtact tgccgatatg ggaattgccg cgatcgacga gatggacaag atgcagaagg
     2881 aagaccggag tgccctccat gaagcgatgg aacagcagtc gatcagcgtc gcaaaagccg
     2941 gcatcactgc cacgctgaaa tcacggtgtg ccctgctcgg tgctgccaat cccaagtacg
     3001 ggcggtttga tgattttgcc cccatcggtg agcagatcaa tatgcctgcc tctctcctct
     3061 cccggttcga cctgatcttt atcatgggtg acaagcccga tgagaaaaag gacggggcga
     3121 ttgcggagca catcctgaaa gcccacagta tcggcgagac gatcgtgcag caccgcaagg
     3181 cacccatccc cggggtgaat gaggagtata tcgccgaaca gctcaaaccg gtgacccccg
     3241 agatcgagcc aggtctcttc cggaaatacg ttgcctatgc gaaaagaacg tgtttcccac
     3301 ggctctcgga tacagcacgg gaggctctga tcgcctacta catgcggctc cgggggctgg
     3361 catctgaatc caacaagccg gtcccggtga ccgcacggca acttgaagca ctggtccggc
     3421 ttgccgaggc cagtgcgcgg atccgcctct cccgggatat cgaggcaacc gatgccgaac
     3481 gggttatcac catcgtggac aagtgtcttc ggcaggttgc ctacgatccc aagaccggtt
     3541 cgtttgatat cgacaaggtt gtcaccggct attccaaggg caagcgggat ctgatccgga
     3601 ggataaagga agtgatcagg atggttgctg atgagagcgg cagggcaaag gtcgatgagg
     3661 tcatcgagca gattgcacag gaaggattcg aaaaggacga gatcaggaag cagatcgaaa
     3721 tgttcctccg ccagggcgag gcgatggaac cgaaacccgg catcatcaag ctcatctgag
     3781 catctccttt tttcccggaa aaagtctata ccagatcatg gcaagtgagc atgatcaggc
     3841 aatatttgag gcagcgatca aactcggggc gctgtaccac cagtgggttg ggacacccgt
     3901 ctcaccttcc acggcatcac tccttgaatc cgcaattcag gagagcgtca gcctccagcc
     3961 ctatgttgcg gatatccatg tctcccttga ccggtcgcag atggcggaga acatcttcgg
     4021 ctactccgag ctccggggca ccatgctgac cgtggacatc atcaccaagg taggcagctc
     4081 ctgctgccat gccaggctgc agcgaagagg agactacccg ctcatggaga tcgtctcgtg
     4141 ccatgaagag ccataagttt ccgatcaccg acgaccatat ccatatcgat cctgtcaacg
     4201 ggaggggact ggaagccgcg aaggattttc tccgtgccgg gggaacccac ctcttccttg
     4261 tcacgaaacc ctcctcgtcc ttcggtgttc tcccccatac cggtgatgaa ttttctcttg
     4321 tttacgaaaa gaccatcgcg attgctgacc agatcagggc tctcggtatc acggtctttg
     4381 tcattctggg ggtacatcct gctgagatga cacgcctttc agccactacg ccgctctccg
     4441 agaccgtggc aatcatgaag agagggattg atatcgcggc ctcttatatc ggagagggaa
     4501 gagcggttgc gctcaagagc gggcggcccc attacccggt ccccgatgag gtgttctccg
     4561 cctccaacga ggtgctctcc catgcgctga cccttgccgc cgatctgaag tgtgctgttc
     4621 agatccatgc ggagactggt ccctgcagcg acgttgtcgg gatggcagaa caggccggtc
     4681 tgccctccca taaggtggtg aaacattatg caactccgga caccccgctg acaccctctc
     4741 tcctggcctc ccatcctgat atcccgcaac tcgcccgtct ccgccggccg tttaccatgg
     4801 agaccgatta catggatgag aacacccggc cgggtgcggt gatcggtccg aaatcggttc
     4861 ccaggatcac cagaaagctg atcaacgaag gcctcatctc tgaggacgac gccttcagaa
     4921 tccacaaaga aacacctgaa agggtctatg gcgtcgagat catcctctga accaccagcc
     4981 cacgtctcac cttttaagaa ctcgcctctc ccatagtact gatcgatgta cctgaaagtt
     5041 caccggacac cggccggtga tgaagtcgtc gctgtctgcg acagggaact tcttgacaca
     5101 tgcatccagc agggtgacct cgagatccat atctccgggg atttttacgg aaggagccct
     5161 gcatcaccgg atgaggtgag agcagccctc aggggtgcag ggaactgcaa cctctttgga
     5221 gagcggactg ttgcccttgc catcgagatg ggacttatcg atgcgacagg atttatccgg
     5281 attggatcag taccccatgc ccagatcatc aggctctgaa aagaagataa gggagaatat
     5341 ctgcccccgt tgcggggctg catcagccac cggtgagctc tgtggcagtt gcaggatcca
     5401 tgagaacccc tggttcacgt gcggtaaacg ggttgtctgt atcctttgcc cgacatgcgg
     5461 atcgcggaag gagggaggtg tctggaccga taccgttgca gaacgggagg agattgcccg
     5521 tgatctggta caacgctcgg tgcagatcgt acccgaggtt gcccggccgg tcctgaagat
     5581 ctccgtaaag gagatgacga tcaaccgctc ccttgccgag gtggtactcg agggaaccct
     5641 gtttggggaa ccggtgcatg ccatgtgtca ggttgagatc ctctggcaga aagagcagtg
     5701 tgaccggtgc agccggatct ccgggagcta ctacgaggga atagtccagg tgcgggcctc
     5761 ggggaggaga ccgtaccgcc acgagatccg gaccgtggag cggatagcca gggagatcga
     5821 ggaagggtgc tatgcttcag gggaacgtct ctcgttcatt tcagacactg acgagacaaa
     5881 ggacggactt gacatcaccg tcggttctca gcatatcggc caggcgatcg ccactgccat
     5941 cgtccagcga ctgggagggc gcttttccac ccatccaaaa ctggtgggcg aacgggcagg
     6001 aaagcggctg taccggatca cgtattcggt ccgtctcccc cggtacggga agggagatgt
     6061 catcgtggtg aatggcagac ccggtgaagt gatctatgcc tcggggaggg agatcagata
     6121 ccgggagatc ccgaatggca tcgaacgctc ggcacgggag gaccgggtcg aacggtatat
     6181 cggatcgacc agggatgcac gggagtacct ggtcacgttc agacagggga acaccatcgg
     6241 tgtccttgat ccggagacag ggatcccgcg ggagtgtttc gtcggccggg acgcagaata
     6301 tgtaccaggg cagacgatca ggatcctgaa tgacggggat gacctcctgg tggtgggtga
     6361 acgatgaggg tgagagaggt tcccgcccgc gatcttccag ggattgcaga agcagaatgg
     6421 cgaatgaggg agagaaggcc ctttgtcagc aatgggatcg cgtatgtgcc ggtcagggac
     6481 gggtatcctg cggacaggga actcgccccg aaagcacggt acgacggacg gggcttttat
     6541 atgatcggga cgattgccgt ccttcacggg agagaaccca ccagtgccga gatacgggag
     6601 attgaagcgt ggaagaaacc gaccggcatc ctctggatca ggtcatacca gggcagggaa
     6661 cggatccccg atgtccgtct catttccgga tcgtccggag agatctgcca ccatgaactg
     6721 ggaatggtat tctggcttga tcctgcacag gtgatgttct catcggggaa ccgggcggag
     6781 aagcagagga tgatgaggct aatacgcccg ggtgaacggg tagccgatat gttcgccggg
     6841 atcgggtatt tcagcattcc cgcgggcatg gccggtgcca ccgtccatgc aatggagata
     6901 catccggttg cctaccgcta ccttctcaga aatatcgatg aaaaccacct ggagaaccga
     6961 atttttgccg ggcttgggga ctgcagggaa ctcctctccg gtacctatga ccggatcatc
     7021 atgggtcatt tcgactcctc cctgatgctc gatgccgcgt ttgatcatat caggaccggt
     7081 ggcacgatcc atatgcacac cgcaggcagg aatcctcctt tcatcccgga ggagtaccgc
     7141 gatgcctgcc gggatgcagg aacggtcagg agaataaaaa aggttgcccc gcacacatgg
     7201 cattatgttc ttgacatgag ggctggatga tggaaccagg aagccttttc aaaaaacgga
     7261 tcatcctcgg ggtgaccggg agcatcgcag cggtggagac cgtccgcctc gcccatgcct
     7321 tgcgacgcga aggtgccagc gtcaccgctg tcatgagcga ggcagcgacc cggatcatcc
     7381 acccggatgc attgacctac gccaccggga atgatgcgat cactgcgctc tccgggcatg
     7441 tggagcacgt cacgttctgc ggggacgggg gatgtgcgga tctgctgctc atcgccccct
     7501 gcacggcaaa cacgctctgc aagattgccc ggggcatcga tgataccccc gtcaccacat
     7561 tcgcaagcac ggcgctcggt gccgggctgc cggtcgtggt cgcccctgcc atgcacgaga
     7621 gcatgtaccg gcatcctggc gtgagcgagt gtatcaggtg tctctcctca tggaaggtca
     7681 cggtcatccc ccccaggaga gaggagggaa aggccaagat cgcggatatc gatgagatcg
     7741 tcctctggtg ctgccgggca ctgccgggat ctccccttgc aggaaaacac gtcctgataa
     7801 cgagcgggcc atgcagggaa ccggtggatg atatccgcgt tctgaccacg cgttcaaccg
     7861 ggaggatggg gagggaactc gccctccagg cgttccgcct cggcgcggag gtcacggtgg
     7921 tccatgcagg ggtgatcccg tgtgtcagga atattcccat cgaaactgca ggggagatgc
     7981 acgatgccgt gcacaggata tgcgctgagc ggatcccgga tatctacctc agtgccgcag
     8041 caatctcgga ttatgccccc gaaccatacc gaggaaaact ccggagcggc cagacactct
     8101 cgatccccct ccacccgctc ccgaaactga tcgatgaagt ctgcaggaga tacaatacct
     8161 cggtcattgc tttcaaggtg ggagaggagg gggaggaggg agcagccccg ctgattgcga
     8221 agggtgcaga tcttgtcgtg ataaacggcc cggagagtat gggatccgat accacggatg
     8281 ctatcatctc tggcgctggc ggcagggagc agatacgagg aacaaagagg gagcttgctg
     8341 cccggatctg ggagaggata gggacgctgc tcctccccca gggctgacgg ggaaaaattt
     8401 gcgaacggga agagaacgat gaggagatca ggcaggagtc cccaagagcc tgttgctgca
     8461 tcggtcactg cttcctgccc gggccacatt tccgggtatt tcaggccggt gaccgggtca
     8521 ggataccaga cgaccgggag tatcggggcc gggatcgtga tcacggaagg ggtgaccgtg
     8581 cgggttactc ccgccgatac cacctccatt acggtccggc gtctctcctc tgaaggagct
     8641 gtccgggagc tgatccatga tgctccccct ctcgcctatg ttgccggccg cctcggtgtt
     8701 ttcgcgcaca tcgagaccga atgcacacta cccattgggg caggatttgg gctttccgca
     8761 gcagcactga tggcctcggt ctctgctctc aatgaactct tccatctcgg gatgacaaag
     8821 aaagcctgct ccgaacttgc ccatgaatcg gagatcgttc accggaccgg gcttggtgac
     8881 gtcgccgcat gccagggagg cggtcgcgat ttccgcaggg gaccggggac aggtgctgag
     8941 atcaggaggt tttatgacct gcacgagccc ctgtatgctg tcaatttcgg accgctcccc
     9001 tctcccctgg tccttggctc accggatgca cttacgcgta tcagtgccgc atatcctgaa
     9061 gagtatcccg gatcccctga actgttcttc cggctctcgt acagatttgc cagagggagc
     9121 ggactgctca cgcaggaact cagtgacctg ctcgtggaat gcagcagaga gaacatcccg
     9181 gccagcatga ccatgctcgg gaacgggatt ttcgcgatgg ggaagcgtgc cgcggacctc
     9241 ctcgggtcct atggtgagat actggagctc catgttgctc cccgcggcgt tgagatcctt
     9301 gaggagttct catgatcccg gaggaccatc cccggtaccg ttcgcttcaa gtgagggagc
     9361 atctcgcccg gtgcgcagcg gagggtatcc tttcgcttga agggcttact gctcacggcc
     9421 gcggtgaagc gtttgattac ctgatcggtg aaaagaccat ggagagtgcc ctccgggcgg
     9481 agagggccgc tgcagcgctc ctcctctgtg cggaacggcc ggtcatctcg gtgaacggga
     9541 acacggcagc gcttgccgcc ggcagtattg cgctccttca gcaggcctcg aacgctgcgg
     9601 ttgaggtgaa cctcttccac aggactgaag agcgaatcag gaagatcaca accctcctgg
     9661 aacgccatgg agtcacggtg ctggagggca tcccggaacg actcctcccg ctctctcatg
     9721 cccgggcgct ctgcctgccg gggggaatcg gttccgccga tgttgtcctg gttccccttg
     9781 aagacggtga ccgctgccag gcactggtcg acgcgggaaa gaccgtcatt acgatcgacc
     9841 tcaacccgct ttcgagaacg gcgatgacgg caaccctgac cattgtcgac gaggtaacca
     9901 gggcgatccc gcaggtgacc cggttctgca gagcccttac aagggaagaa gtgcaactgt
     9961 gtgcaacagg atatgagaac cgggaactcc tccggtctgc catccgcgag attcaattga
    10021 ggctcgacca tgctctggat tgaaaaatac cgcccgaaga gttttgaaga gatacggggg
    10081 caggatcacg tggtctctca cctgaagaga gcagcacgat caggggatat cccgcacctg
    10141 ctcctcaccg ggccaccggg caccggaaag agcgttgcgg tcgaatgcct cgcataccag
    10201 ttatatggtg agaggatggc ggagaacatg acggtcatcc agaccgctga tctcttcgag
    10261 caggggaaga agtatcttga aggggacgag cggtacggac acctctacca gagagaacag
    10321 agtctcctct cgaatttcaa gaatatagtc agatggtatg catcgatccg gcccctcgat
    10381 tctgacttcc gtatcatcgt ctttgaaggg gcatctgcga tgacccggga agcccagcag
    10441 gggatccgga gaatcatgga acggtattcg aggacctgcc gcttcatctt tgtcacgagc
    10501 aggccatcgg cgctcatacc ggcgatcagt tcacggtgtc tcccgttctt ttttgtgccg
    10561 gtggaaagca gggtggtcgg agaccacctc catgccatcc tggcatcgga attcggcgat
    10621 accagccccc tttcagatga tgataccgac ctgatcgtgc aggctgcagc cggtgacatg
    10681 agaaaggcgg tggtgctgct ccaggtcagt actggtgaag aatgcctggg caacctcatc
    10741 aggtgctccc agaccgagac aggccagatt gcgcaggcag ccctgaccgc ggtatccacg
    10801 agtgacatga tctcggcagt acgccggttc gagaccctca tgatcgagta cggcctcacc
    10861 tccagggagg ttctccatga attccgcaca gcggtcagga gggagtataa cgatcccagg
    10921 ctctcaatag cgattgctga taccgattat actctctccc atggcaacaa tgaatacctc
    10981 cagctcaatg ccatgctggc ccgcatcatt gcggaggtat tctcctgacc gcgggcaggt
    11041 ggatcagttg acggaagaga gataacggat aatccttctc ttcttctcga tagccatgtc
    11101 tgctgctttt tccacggcca ttttcattcc tgccagatca gccggatgcc ggtccgccac
    11161 tttcttcacc ggcgtataag cagactcccg gaaccgggga gagaaatcct gctcttttcc
    11221 gtcactcacg agatatgcac cggggctccc ttgctcgaca cgcccgatct cctcgatgcg
    11281 tacaccggca tcgctgataa cctctctgag cgccggtacc gcatctccag gtgccacaat
    11341 caggagtgaa tcgagcgaga caccgagata atcgatcgca agggcatcca gcatctcctg
    11401 gaccttcgca tcggcaatac ccctgacacg atcttccacg ataacgacac gacagccggc
    11461 ggtctgggca agttcatgcg catcgccgcg gagaccaccg ttagtcacat cggtcatggc
    11521 atgcacctcc ccgatcacgg gacttcctag gagagcttcc actgctttca ggaactgcag
    11581 gttgatggtc cgctccacga cctccggaaa tcccgaataa atagcggcag tggcgatcgt
    11641 tccccctccc gcgccttcgg tcatgaggat cacatccccg ggtgctgcac tcttccttgg
    11701 ggtgaggtgc cgggcgatgc caaccgttcc cacgcacccg gtcatccgtt ccccgagcac
    11761 catgtcgccg ccaatccgga gcgttgaccc ggataccagg ggaactccca tcatctctcc
    11821 aacggtggtt atgccagcgg tgtactcaaa aatcttggca acgtccccgt catctgccac
    11881 atggatatca gagaagagca ggatcggccg tgcgcccatc acgtaggcat cgcggagcgt
    11941 cgcccgtgtc gcgtggaatc cggcaaggaa agggaaatca gagaggcgcg agtgcatccc
    12001 atcgaccgca cagacgatgt acctgccacc cgcggttcct gcaacctcta ccacccctcc
    12061 gtcatccatc tcatcaacgc caaccgcagc ccgggttctt ccgatcacac gggctatctg
    12121 gcgatgggcg aagaaatcgc cgctccctcg ggaaccaacc ccgaattccc ccatgctcac
    12181 acctgaatga tgatatgaaa aaaagtcacc gccgatcccg gtggaatttt ttacctcgtt
    12241 gatgaccgcc tgtgcaaacc gttctgcata ctcctgatcc tcctccggtt taaagacccg
    12301 gatctggttc gcaagggctg cacggagatc cttttcgaag attccctggc gaataccacg
    12361 gcggacgtac tcctcaacat ccatgtggct atcttttgct ttgcgcagta ataaggctag
    12421 agcgtaccgc agcgatcggt caggagcatg caccgaggga ccatggcggg gaagaggaga
    12481 atgatggtga aaaaacacct gttctgatga agatcatgaa aaaaattgag aatttgtttg
    12541 aagattacgg tctgtgtgga taaccgggcc ccttctttcc ccggggcatt ctggatattt
    12601 cccgggagac gggtatgaag atatgcacat cgtaaatggt gaacagaagt gcatggcatt
    12661 tcgataggtt aacttattta agttaaccca taaattaagt taatatgatg cccgaaattg
    12721 aaaaagaacg attagataat tatatctctt gacaggaata acagccagtc gtataaaggt
    12781 gttttcatgg acatgaagta tgtacagacg acctgcccct actgtgggac gggttgtacg
//
LOCUS       DAOOCO010000036        12734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4563_length_12734_cov_5.163420, whole genome shotgun sequence.
ACCESSION   DAOOCO010000036 DAOOCO010000000
VERSION     DAOOCO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4563_length_12734_cov_5.163420"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..999
                     /locus_tag="PK154_03400"
     CDS             1..999
                     /locus_tag="PK154_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPW10141.1"
                     /translation="METLPALLAGAVLVAAFSIIIGLLLMGFDRIVAARMQARIGPPL
                     TQPFTDVRKLLAKENVVPENAIPWLFNLAPIIALASAITILFYLPIGGFAPVLSGGGD
                     LILVMYLLTLPALALVAGGFASGSPYATVGAQREMVTMIAYEFPLAIVVVAIAWRLAA
                     AGISLPFSLATIGANPVWGLVGPLGAIGLLILLIVLLIVTPAELSRVPFDTPEAETEL
                     AGGILVEYSGKNLALFSLTQGVKTVAMASLVVALFFPYRLAAIAGISGFVGAVIDLLF
                     YILLITLVAFISVSVIRVSMARFRINQVVSVYWIYLAIAGLAGLLLVMVDQIMGVV"
     gene            1001..1405
                     /locus_tag="PK154_03405"
     CDS             1001..1405
                     /locus_tag="PK154_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW10142.1"
                     /translation="MAGLPLLPELVRQLFSRPATNAFPSRHLPSSITGFLAKVARGEA
                     TIHPPIPTPPKFRGKIVYDREACIGCSLCLRVCPAHAIEFIPETKRVRIYVTQCIFCS
                     QCNDVCPKNALHMSHEFLLATEDRYAESQIVE"
     gene            complement(1449..1928)
                     /gene="hycI"
                     /locus_tag="PK154_03410"
     CDS             complement(1449..1928)
                     /gene="hycI"
                     /locus_tag="PK154_03410"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HPW10143.1"
                     /translation="MVIVRMLLGIGNNLHGDDGIGNYIASRFRARGWISLDCGTMPEN
                     FTAVVRRHHPEMLVLVDATDLGLEPGEFRSVGEDQIEEVSIGTHNLPLSFLIRYLAES
                     AGQVIFIGIQPGHLGDSEGISPAVTAGAEQFIALLAQNRIPEIKSFKGREKRDERGD"
     gene            2184..2750
                     /locus_tag="PK154_03415"
     CDS             2184..2750
                     /locus_tag="PK154_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPW10144.1"
                     /translation="MTDETTTTMGMCNIFGGFFPWWVLLIQGIVALILGIFFLTSPAM
                     TVLVLITFLGAYWFISGIFTLFSAAGEQKDRGMKILLGILGIILGLAILAYPFYSAFI
                     VPFIFIIMVGVWALVIGAIHIYQGITGGGWGVGIIGLLSVLFGLILLVHPYLATLALP
                     FVLGVLGIVLGFAAIIGAFMVRSKQNAA"
     gene            2889..3776
                     /locus_tag="PK154_03420"
     CDS             2889..3776
                     /locus_tag="PK154_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10145.1"
                     /translation="MFELFFEIGNYAIWIFVVTSIAAMGLNLTVPEVLAPWKKKWLLS
                     ISLIANFVVVPLFALFILYLFPLDLELATGLILVAAAAGAPSLPKAMELVGGNVAYAV
                     GLTMILILVTIFYMPFMLPYLIEGVQVDQTSTAMYLIVFMLVPLLLTMAIRAKFPLLA
                     YRLYPVIDRVSDLSIVVVLLIYTVALFMSDFSVKAGTLLGLEGIAVAILFILGSFAIG
                     YTLGGPELQNRQVLAFGTGFRNVSAALVVVSASFTDPQILFMVLVIAVFGIVFMFITG
                     GWLYRKNKKEGKRNPARDL"
     gene            3990..4451
                     /locus_tag="PK154_03425"
     CDS             3990..4451
                     /locus_tag="PK154_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPW10146.1"
                     /translation="MVLRRVFVFCILVLFGLLFTGCSAPAPMPEVEVKGAAMSSISLA
                     EMAIEVFLTVDNPYPVAITLQSVSFDVYYRNGDEWKYLAHGEQADIPVNPGENPVTIP
                     IKIKNADLLGSLLSFADSRQILLEIRGVVVPDLPGNPAPEIPFTKTVSIVR"
     gene            4783..5826
                     /locus_tag="PK154_03430"
     CDS             4783..5826
                     /locus_tag="PK154_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW10147.1"
                     /translation="MNSNQKMSEVSRILIPLAAFVIVLAGMKAAVSIVGPILFSIFLT
                     VIFGMLLHWFERKGFTTRNAFILTLAIFFAILAVFIVVIAGSFLQLLSDLPRYQQELE
                     ASLELISPYLISVGIDPSSLTLQEIVTSLSIEIGSLIRQLWNIAILFVMIILTTIFLL
                     FEARGFSQKLRIIIEQLRPGDLPRFTALAEKNVDYLIIRTEVNLAMGIGTAVILALIG
                     VKYAILWGFIAFLLGFIPYIGFWLAVIPPMLLAWFDLGPLAAILVLAGSGVVNLMAEY
                     LMFPHLAARGLALSTAVVFISLIFWGWLLGGFGVLLAVPLTLSFQMICELFDETRWIS
                     VLLGPSPGKHKRP"
     gene            6028..6639
                     /locus_tag="PK154_03435"
     CDS             6028..6639
                     /locus_tag="PK154_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10148.1"
                     /translation="MEKSYGIYLAYFFMALIALNAVRALFRGEYNAMVTAVLMLGMTV
                     IPFVVAKRMNINLPWFVFFLVALALWIHTAGYVQGYYTTFYPYYDKIAHIVSGTAVAV
                     LGFLGVIFVDKYMKMNLNTWFIIFFTLIFGMAMGAAWEIYEFLVDFFFGGSLAGPMQN
                     SLNDTMLDMMFVLAGSIVVALLGVFWFKQHRKEEITQGAKIPV"
     gene            complement(6718..7413)
                     /locus_tag="PK154_03440"
     CDS             complement(6718..7413)
                     /locus_tag="PK154_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPW10149.1"
                     /translation="MFIIGHRGARAVEPENTLRAMKTGMTCADYIEVDVRLSCEGIPV
                     IMHDPMLDRTTNGTGSVNAHTLEELKSLDAGKGESIPTLEEVCRLVSGRCGLFVEIKE
                     PGSEEAISRVIREFGLKDLFVVSFHQESIIAALAHLPGVRTGIIVSKETASLPGAAQE
                     LGTDAVLPKFALLNDALIAECHARDLLVVSWTLNTPEEFMVAAHLGIDGFASDDPCKV
                     RRYFKDRKIFTRI"
     gene            complement(7544..7879)
                     /locus_tag="PK154_03445"
     CDS             complement(7544..7879)
                     /locus_tag="PK154_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW10150.1"
                     /translation="MGREDREEIKGTVLRLKELVAYQDGSIASRMIINSKAGSITLFS
                     FDREEGISEHTAPFDAVATILDGECEIRLSGVVHLLKEGETIIMPAHAPHALTAITPF
                     KMMLVMIRE"
     gene            complement(7972..8766)
                     /locus_tag="PK154_03450"
     CDS             complement(7972..8766)
                     /locus_tag="PK154_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW10151.1"
                     /translation="MVFEQARAIGFIYALVMFFILAYLWYTKRWTRRIGWIILLITVA
                     LGFLIFSPIIPWQFQSLVLRDARGVGGPLAIAAAGLIVMLALSFVFGRFYCGYLCPVG
                     AFQELASLAPVSKIKVGSKVWPGAIRWIFFILFIIAGYFFTYGLLRLFGIRDFFLLLL
                     SAGFFIFLAIIAISLFLYRPFCRFICPFGALASVPAMGSRFKIRRTNACISCGKCERA
                     CPTNEAKRDDRKGECYLCHRCLDVCPVEGALEYRRSGGEPGKHGAK"
     gene            8855..9568
                     /locus_tag="PK154_03455"
     CDS             8855..9568
                     /locus_tag="PK154_03455"
                     /inference="COORDINATES: protein motif:HMM:NF021420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HPW10152.1"
                     /translation="MIEGRWLADARRRQVEYDPGEMKLGRESSRTGDDDPMSLVVAFS
                     GHDGAVIAGDLREILMQGPDAETAGFEQELYSGQIVNDSDLGKRAAERGIALQVRDDK
                     CKVSDQGGILVGEVSESDGATVRIRRLFVSCGTYAIVDVTEGKQVLQKTGKGSSFVVL
                     GNEVTKRIAHDMIQEEWKNGTFADAVRIIIGIMTSAASCTASVSRAYTILQTRRRADL
                     DIATIMNYGKGGRDQEGGW"
     gene            9578..9895
                     /locus_tag="PK154_03460"
     CDS             9578..9895
                     /locus_tag="PK154_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10153.1"
                     /translation="MSPIKRGHESPCYPGQNLSLSSYTGLFTDKNIPLHDTNPHPWIF
                     PAHPAKGKYVEGKGSIRIPVAIAGEIRHDRRMVTIVPCHFQERERHLLPNNPDLAWKE
                     LGS"
     gene            9910..10179
                     /locus_tag="PK154_03465"
     CDS             9910..10179
                     /locus_tag="PK154_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10154.1"
                     /translation="MIIKQCFPLVIERYERDPVSPEASIGSLERYRKMGYDAIRNLPQ
                     EEKERDQSAIDTAFQESAEKIQRLDEQRRQHCADTQNADDLPVQS"
     gene            10200..10562
                     /locus_tag="PK154_03470"
     CDS             10200..10562
                     /locus_tag="PK154_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10155.1"
                     /translation="MARTLSERDLAILSLLAPEVTDPLCPGSGHDFFSILPPVSNHFA
                     TDDADFLNRIEQLPDEDLTYLAGLILDGKESIGCMRPEHVVLLADEIARRISMETADS
                     IIGLYAAGVACGNEDDEE"
     gene            complement(11342..12535)
                     /locus_tag="PK154_03475"
     CDS             complement(11342..12535)
                     /locus_tag="PK154_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPW10156.1"
                     /translation="MNTRVPRDELDNRMKRFRVQMDTIHPDWEIATVFSKVNLYYFCG
                     TIQDGMLLIPRDGEACLWVKRSYERAMDESLFPQIKPMRSYRDAVQSIPSIPDTVYLE
                     TEQVPLALFSRLRKYFPFKYVRAVDKQILQIRSVKSPYERGLMERAGEIHRIVLEELV
                     PGALTEGISEAEFTNELYRLMMEEGHHGIVRFGMSESDIMLGLVGFGESSIYPTYLDS
                     PGGNAGLSPAVPLLGSRDRNLHDGDLVFVDIGCGFHGYHTDKTMTYVFGRSLPKEVVE
                     IHRRCVKIQDTVASHLKPGAIPSQIYESVMGELEPSFLDNFMGYGDRKVQFLGHGIGL
                     EIAEHPVIAKGFDHPLEEGMVISLEPKKGIKGVGMVGTENTFVVTSNGSRSITGNHPG
                     LIPVG"
BASE COUNT         2809 a         3481 c         3240 g         3204 t
ORIGIN      
        1 atcgaaaccc tgcctgccct cctggccggg gctgtactgg tagcagcatt ctcgatcatt
       61 atcggcctgc tgctcatggg atttgaccgg atcgttgctg cacggatgca ggcacggatc
      121 ggtcctcccc tgacacaacc attcaccgac gtaaggaaac tccttgcaaa agagaacgtc
      181 gtgccagaaa atgcgatacc atggctgttc aacctcgcgc ccatcatcgc cctggcctcg
      241 gcgatcacca tcctcttcta tcttccgatc ggtggctttg cccccgtatt atccggaggc
      301 ggcgacctca tcctcgtgat gtatcttctg accctgcctg ccctggcgct ggttgccggg
      361 ggatttgcat ccggttcacc ttatgcaacg gtcggtgccc agcgcgagat ggtgacgatg
      421 atagcatacg agttcccgct tgctatcgtg gtagtcgcca tcgcgtggag actcgctgcc
      481 gcaggcatct cccttccctt ctcgcttgcc accattgggg ccaatccagt ctggggcctt
      541 gtcggtccgc ttggggctat cgggctcctg atcctcctga tcgtcctgct tatcgtgacc
      601 ccggcagagc tctcccgggt ccccttcgac actccggaag cagagactga gcttgccggg
      661 gggatccttg tcgaatactc cgggaagaac ctcgccctct tttcattgac gcaaggggta
      721 aagaccgtgg cgatggcctc tctcgtggtg gcacttttct tcccttaccg ccttgccgca
      781 atagcaggta tatccggatt tgtaggagcg gttattgatc tcctgtttta tatcctgctg
      841 atcaccctcg ttgcctttat ctcagtgagc gtgatcaggg tctcaatggc acggttccgg
      901 atcaaccagg ttgtctcggt ctactggatc tatcttgcca tagctggtct ggctggactc
      961 ctccttgtca tggttgacca gatcatgggg gtggtctaaa atggccgggc tcccccttct
     1021 ccccgaattg gtaaggcagc tcttcagccg gccggcaacc aatgcattcc catcacggca
     1081 cctgccctca tcaataaccg ggttcctggc aaaggtcgcc cgtggagagg caacaatcca
     1141 cccacccatt ccaacccccc ccaaattccg tggaaaaatt gtttatgacc gtgaggcgtg
     1201 tatcggctgc tccctctgtc tcagggtctg ccctgcccat gccatcgagt ttattccaga
     1261 gacaaaacgg gtcaggatct atgtcaccca gtgtatcttc tgctcccagt gcaatgatgt
     1321 ctgcccaaaa aatgcactac acatgagtca tgagttcctg ctcgcaaccg aagaccggta
     1381 cgcagagagc cagattgtcg aatgacaatc tggccgtcat catttttccg gccatcaacc
     1441 cagactgatc aatccccccg ctcatcacgt ttttccctcc ccttaaagga tttaatttca
     1501 gggatacggt tctgtgcaag cagtgcgatg aactgttctg ccccggctgt cacggcaggg
     1561 ctgatcccct cgctatctcc cagatgcccc ggctggatac caataaagat cacctgtcct
     1621 gccgattcgg caaggtatct gatgaggaac gacagaggca ggttatgagt gccaatgctc
     1681 acctcctcga tctggtcctc accgacactc ctgaattcgc cgggttcaag gccgagatcg
     1741 gtcgcatcca cgaggaccag catttcggga tggtggcggc gtaccactgc cgtgaagttc
     1801 tcgggcatcg tcccacagtc gagcgatatc cagccccggg cacggaaccg tgatgcgatg
     1861 taattcccta tcccgtcatc accgtggagg ttgtttccga tcccaagcag cattctgact
     1921 attaccatca cccgagatga tttcccaatt gtattgtcat ggcaggatga aaaatcaggc
     1981 acctgtatgc caggaatgag agcagggaaa ggaggccaat tttggcattc agggaccatg
     2041 cccctgttct gtcctggtaa tacggattcg agatagtaat ttcgtgataa taaaacaatt
     2101 tccagccttt ttttcactgg aaactgaaaa aattttatat tacctggtat caatcttcca
     2161 tgttctgtta ttggagcact actatgactg atgaaacaac cacaacaatg ggcatgtgta
     2221 atatttttgg aggattcttc ccctggtggg ttttgcttat ccaggggatt gtcgccctca
     2281 tccttggtat tttcttcctg acctcccctg caatgactgt actcgtcctt atcaccttcc
     2341 ttggtgcgta ctggtttatc agcgggatct tcacgctctt cagtgcagcc ggagaacaga
     2401 aagacagggg aatgaaaatc cttcttggca ttctcggaat cattctcgga cttgccatcc
     2461 ttgcttaccc gttctatagt gcgttcattg tgccgtttat cttcatcatc atggtggggg
     2521 tgtgggcact ggtgatcggg gcgatccata tctaccaggg gattaccggc ggtggctggg
     2581 gtgttggaat catcggcctt ctctcggtat tgttcggcct gatcctcctg gttcacccgt
     2641 accttgccac actggctctc ccgttcgtgc ttggggttct cgggattgtc ctcggttttg
     2701 ccgccatcat cggggcattc atggtacggt caaaacagaa cgcagcctga aaaccacacc
     2761 atattctctt tttttccgtt ccctgtggaa tgagccggtt ctccttcgcc agactattaa
     2821 gggagttcca tcaaacctaa tcagcgtacg aatgcacatg gtactgcagg gaagaggtca
     2881 cggattccat gtttgaactg ttttttgaga tcggcaacta tgctatctgg atcttcgtgg
     2941 tcaccagcat tgctgcaatg ggtctcaacc tcacggtccc tgaagtcctt gcaccatgga
     3001 agaagaaatg gctcctctcg atctccctca tcgcgaactt cgtggtggtc cctctttttg
     3061 ccctctttat tctttatctc ttcccgcttg atcttgagct tgcaaccggg ctcatcctcg
     3121 tagccgcagc agcgggagcc ccctcgctcc ccaaggccat ggaactggtc ggagggaatg
     3181 ttgcctatgc tgtaggcctc acgatgatcc tgatccttgt tacgatcttc tacatgccat
     3241 tcatgcttcc ctacttgatc gaaggggtcc aggtagatca gaccagtaca gcgatgtacc
     3301 tgatcgtctt catgctggtc ccgctcctcc tcactatggc gatccgggca aaattcccgc
     3361 tccttgccta tcggttgtat cccgttatcg atcgcgtatc ggacctgtcc atcgtagtgg
     3421 tccttctcat ctataccgtc gccctcttca tgagtgattt ttcggtgaag gccgggaccc
     3481 tccttggcct tgaaggcatc gctgttgcga tcctcttcat cctcggttct tttgcgatag
     3541 ggtataccct cggcggtccc gaactgcaga accgccaggt cctcgcattc ggaacagggt
     3601 tccggaatgt ctcggcagca ctggtggtgg tctctgcaag cttcaccgat ccccagatcc
     3661 tcttcatggt ccttgtgatt gcagtgttcg ggatcgtttt catgttcatt acgggagggt
     3721 ggctctaccg gaagaacaaa aaagaaggaa aacgcaatcc agcacgtgac ctctaatgga
     3781 cacgagcctc agctctcatg gaaatgcctc accttatgtt tcctgacaga ccggacagcg
     3841 gtcatgtgag gctcctatga acattgctac gccattttca cgttgtatgc ctgcagcttt
     3901 ttgtatgctt gcggcttaat gcgaatcatg tgattttttt ccctgggcag gggcactgtt
     3961 ttctatccgt aaaggatgat gtatttccta tggtacttcg tagggttttc gtattttgta
     4021 tcctggtact attcggtttg ctctttaccg gatgcagtgc tcctgccccg atgccggagg
     4081 tggaggtgaa gggggcagcc atgtcatcca tctccctggc agaaatggcc atcgaggtct
     4141 ttttgactgt cgacaatcca tatccggtgg caataacact ccagtctgtt tcctttgatg
     4201 tgtattaccg gaatggtgat gagtggaagt atctggccca cggagaacag gctgatatcc
     4261 cggtcaatcc gggagagaac ccagtcacta tccccataaa aataaagaac gccgatctgc
     4321 tcggatcgct cctttcattt gccgatagca gacagattct tcttgagata cgaggagtgg
     4381 tcgttcctga cctgccaggg aacccggccc ctgaaattcc tttcacaaag acggtatcta
     4441 tcgtccgctg aaaacaccga attccgcttc tcccttttcc gggatttcct ctgaaggctc
     4501 gaacagttca tatgttggat aggagcgctg tatcgcggat gtcattcaaa attcaaggtc
     4561 ctgccagagg ggagagaagg atggatcctt tttaacaaca ggaggccagg gacggatttg
     4621 gatccatttc cctcagagag gggcgagccg ggagaggcca cgtcactgtc tgacacaatc
     4681 ttcactgtct gacataattc gtgcaaacgt aagaaaaacg ttaatgccac atcctgtgct
     4741 aaactaacaa ccatccagcc agccacagga agcctttttc ccatgaattc gaaccagaag
     4801 atgtccgaag tctcccggat ccttatcccc cttgcagcgt tcgttattgt ccttgcggga
     4861 atgaaggcgg cggtatcgat cgtcggcccc atactcttct caattttcct taccgtgatc
     4921 ttcgggatgc tcctccattg gttcgagaga aaaggcttca ccacccgaaa cgccttcatc
     4981 cttacgctcg cgatcttctt tgctattctt gccgttttta tcgtggtgat cgcagggtcc
     5041 tttctccagc tgctgtctga tctcccccgc taccagcagg aactcgaggc gagtcttgag
     5101 ttgataagcc cgtatctcat ctcagtcggg atcgatccct cttccctgac cctgcaggag
     5161 atcgtcacct cactctccat tgaaatcgga agcctgatcc ggcagctctg gaatatcgca
     5221 attctctttg tcatgatcat tctgaccacc atctttctcc tctttgaagc caggggattt
     5281 tcccaaaaac tccggatcat catcgaacaa ctcaggccgg gcgacctgcc ccgcttcacc
     5341 gcccttgcag agaagaatgt cgactatctc atcatacgaa ccgaggtgaa cctcgccatg
     5401 ggtatcggga cagcagtcat actcgcactt attggggtaa aatacgcaat attgtggggt
     5461 ttcattgcct tcctgctcgg attcataccg tacatcggat tctggcttgc ggtgattccg
     5521 cccatgctcc ttgcatggtt cgatctcggc cccctcgccg ctatcctggt ccttgcgggc
     5581 tcaggggtcg tgaacctcat ggccgagtat ctcatgttcc cgcacctggc agcacgaggc
     5641 ctggcgctct ccactgcggt ggtgttcatc tcactgatct tctggggatg gctcctcggt
     5701 ggcttcggtg tgcttcttgc agtcccgctg accctttcct ttcagatgat ctgcgaactt
     5761 ttcgatgaga cccggtggat ctcagtactc ctcggaccat ctcccggaaa gcacaaacgt
     5821 ccataacagt gcatccctat ctctgcagct ccatgaattg tcccctgatc atgatgaggg
     5881 gtaattttct tcccgcgctg gagagaggag taagccagga gatggcttcg atcatggacc
     5941 cgttctccgg aacaagggga ggatggtcac gattgtcctt cggtttgtaa gaaggaatta
     6001 attacccggc ccggtgatac cttcaccatg gagaagagtt atggtattta cctcgcctat
     6061 tttttcatgg ctctgatcgc cctgaatgcg gtacgcgccc ttttcagggg ggagtacaat
     6121 gccatggtca ccgcggtcct tatgctcggg atgaccgtga tcccgtttgt cgttgccaag
     6181 cggatgaaca ttaacctgcc ctggttcgtt ttcttccttg ttgcccttgc tctctggatc
     6241 cacaccgccg gctatgtgca gggatactat accacatttt acccctacta cgacaagatt
     6301 gcgcatatcg tatcgggtac agctgtggct gtgcttggtt ttctcggagt catctttgtt
     6361 gacaagtaca tgaagatgaa cctgaacacg tggttcatca tcttttttac cctcatcttt
     6421 ggtatggcga tgggggcagc ctgggagatc tacgaattcc tggtagactt cttttttggg
     6481 ggtagccttg ctggtcccat gcagaacagc ctgaacgata ccatgcttga catgatgttt
     6541 gtccttgcag gctcaattgt cgtggccctg ctgggggtgt tctggttcaa gcagcaccgg
     6601 aaagaagaga tcacccaggg ggcaaagatc ccggtctgaa acgattcttt ccctcttttc
     6661 aggtcttttt ttataggaac atggctttgg catgctccat ttgtatgctc ttctcaatca
     6721 gattcgtgtg aaaatcttcc gatccttaaa gtatctccgg accttgcagg gatcatccga
     6781 ggcaaatccg tcgattccca ggtgtgctgc caccatgaat tcttccggtg tgttcagggt
     6841 ccaggagacc acgagaagat cccgggcatg gcactcagcg atcagggcat cgttcaacag
     6901 ggcaaacttc ggaaggaccg catcagtacc tagttcctgt gcagcacctg gcaggctggc
     6961 cgtctccttt gatacaataa tgccggtcct cactcccggc agatgagcga gcgcagcaat
     7021 aatgctctcc tggtggaacg agacgacaaa caggtctttt agtccaaact ccctgatcac
     7081 ccggctgatc gcctcttcgc tccccggttc cttgatctcg acaaaaaggc cgcagcgtcc
     7141 agaaacaaga cggcagactt cttcaagggt cgggatggac tccccctttc cggcatcaag
     7201 gctctttagt tcctcaaggg tatgggcatt caccgatcct gtaccattgg tggtgcgatc
     7261 aagcatcgga tcatgcatga tgaccggaat cccctcgcag ctgagccgga catccacctc
     7321 gatataatca gcacaggtca tccccgtctt catggcccga agcgtattct cgggctccac
     7381 tgctctggca ccacgatggc ctatgatgaa catgggactc ctcctacaaa tctgcggtat
     7441 gctaccccaa aacggcctga attgagaagt gagtccggtt ggggatgatt tttaccaacc
     7501 tccggttgtg ccccacatga ctctcacgac acaagggcgg gagttattcc ctgatcatga
     7561 caagcatcat cttgaacggg gttatcgctg tcagggcatg cggggcatga gccggcatga
     7621 taatcgtctc cccttccttc agcaggtgga ccactcctga gagcctgatc tcgcattccc
     7681 cgtcaaggat ggtggcaacc gcgtcgaatg gggcggtgtg ctccgatatc ccttcctccc
     7741 tgtcaaatga gaacagggtg atacttcccg ccttcgagtt gatgatcatc cggctggcga
     7801 tactcccgtc ctggtaggca acaagctcct tcaagcgcag gaccgttccc tttatctctt
     7861 cacggtcttc tcttcccatc cttgtcttcc tccactctct tctctcttct caaggatatc
     7921 agttgcgtct cctggcacac atgaatggca gttcttcctc tcgaaaaagt gttattttgc
     7981 cccatgtttc cccggctctc ccccagatct cctgtattcg agggctcctt caaccgggca
     8041 gacatcaagg cagcggtggc agaggtaaca ctcccctttc cgatcgtccc tctttgcctc
     8101 atttgtgggg cacgcacgct cacactttcc gcacgagatg caggcattgg tccgccggat
     8161 cttgaaccgg ctccccatgg caggaaccga ggcaagagcc ccgaacgggc agatgaacct
     8221 gcaaaagggg cgatagagga agagagagat tgcgatgatt gccaggaaaa taaagaatcc
     8281 tgcagacagt agaaggagga aaaagtcccg aatcccaaag aggcggagga ggccataggt
     8341 gaagaaatac ccggcaatga tgaagagaat aaaaaaaatc cacctgatcg ctcccggcca
     8401 cactttactc cccactttta ttttcgatac gggtgcaagg gaggccagct cctggaaggc
     8461 tccgacaggg cacaggtacc cacaatagaa ccttccgaag acaaaactga gcgccagcat
     8521 gacgatgagg cctgccgccg ctatcgccag gggtcctcca acgccacggg cgtccctcag
     8581 aacgagactc tggaactgcc aggggatgat cggtgagaag atcaggaagc cgagggccac
     8641 cgttatcagg agaattatcc agccgatccg ccgcgtccac cttttcgtat accagagata
     8701 tgcaagaata aaaaacatca caagggcata gatgaaccct atcgcccggg cctgctcgaa
     8761 taccatagtt gtctccagca tacgtgtaca attttccctt taatagaata ccggctccag
     8821 gggattatgc agtttccctg cagtcttgca attcatgata gagggcagat ggctcgctga
     8881 tgcccggaga agacaggttg aatatgaccc aggggaaatg aagctaggca gggagtcgag
     8941 ccgtactgga gatgatgatc ctatgagcct cgtcgttgca ttctcaggtc atgacggggc
     9001 ggttatcgca ggcgatctca gggagatcct gatgcagggt cccgatgctg aaactgcggg
     9061 gtttgagcag gagctctact cggggcagat cgtcaacgac agcgatcttg ggaaaagggc
     9121 cgcagaaagg gggattgctc tgcaggttcg tgacgacaag tgcaaggtct cggatcaggg
     9181 tgggatcctc gtgggggaag tgtctgagtc tgacggagcc accgtgagga tccggaggct
     9241 ctttgtctcc tgcgggacct atgccatcgt cgatgtaaca gaaggcaagc aggtccttca
     9301 gaagacaggg aaagggagtt cattcgtggt gcttggaaac gaggtgacca agcgtattgc
     9361 acatgatatg atccaggaag agtggaagaa cggcaccttt gccgacgctg tccgcatcat
     9421 tatcgggatc atgacttccg ctgcatcatg cactgcctca gtaagcaggg cgtataccat
     9481 ccttcagacc aggaggaggg cagatctcga tattgcgacg atcatgaatt atggaaaggg
     9541 tggcagggat caagagggag gctggtgagc aataatcgtg agtcctataa agagaggtca
     9601 cgaatccccc tgctatcctg gccagaatct gtccctttca tcctataccg ggcttttcac
     9661 agataaaaac atccccctcc acgatacaaa tcctcatcca tggatattcc ctgcccatcc
     9721 cgccaaggga aaatatgttg agggaaaggg cagcataagg attcctgttg cgatagccgg
     9781 ggaaatccgg catgatcgga gaatggtaac gattgttcct tgccatttcc aagaaagaga
     9841 gcgacattta ttaccaaata accctgacct agcctggaag gagttgggat cctgaaccca
     9901 aaagtgacga tgatcatcaa acagtgtttc cccctcgtta tcgagaggta tgagagagac
     9961 ccggtctctc cggaggcatc gatcgggagc cttgaacggt accgaaagat gggatatgat
    10021 gcgatccgga atctcccgca ggaggagaag gagagggacc agagcgcaat cgatactgca
    10081 ttccaggaat cagcagaaaa gatccagcga ctggatgagc agagaagaca acactgcgcc
    10141 gacacccaaa acgctgatga tctcccggtt caatcctgaa atgaaacgtc ccgctactca
    10201 tggctcgtac actctctgaa agagatctcg cgatcctctc cctgcttgca ccagaggtga
    10261 cggatccgct ctgtcctgga tcaggtcatg atttcttctc gatccttccc ccggtctcaa
    10321 accactttgc caccgatgat gccgattttc tcaatcgcat cgaacaattg ccggatgaag
    10381 atctcacgta cctcgccggg ctgattctgg acggtaagga gagcatcggg tgcatgcgcc
    10441 cggagcatgt cgttctcctt gctgacgaga ttgcgaggcg gatctccatg gagacagccg
    10501 attcgatcat cggtctctat gcggcaggag ttgcctgcgg aaatgaggac gatgaagagt
    10561 gatctgaagt atacctggat ttttaaggag aatcacaggg ttcagggata attggtaaag
    10621 gtccgcctgc tttcctttat cctgcaatgc tgatacaggc ctttatttgg cgccgttcac
    10681 cggaatgtgg tctttccact gataccgcct taggcagtaa cctgcttttt cgctgaccgg
    10741 gggaggtcag aagtggtttc gatgaactag atgtgacatt tttgtcatat tcctcccgct
    10801 gataaaaacc tcgaacacag cattcatgcc aaaagaagag agtgcctgta tgtcctcacg
    10861 cgttttgttc cccggaactt cgcccacaac agccccgggg gaacgattga tatcgatgag
    10921 gtagagatgg tttgctgctc attttggtcc acttgctgat ctgatggtgc aaagatttgt
    10981 cccagtcgag aacccttcct ggacaggagg ttttgaatgg cattttctgc agattgtgtt
    11041 ctgtcaatgg ccggatcaca cgctggtccc cgatagaaac cccgatgaaa atgcccatca
    11101 ctgaatttct atgggtaaga gaacatacca tcgggaatca cttatttatc acgcgggtga
    11161 ggattcatcg ggtaagtgaa ttccgtcccg atattcatgt gtgggaagaa cgagattggt
    11221 agtggatact ggtttcccca gcacccaagg tgaaatgcag gagggcacat ccagggtatg
    11281 acaaccccag agagagcatt cagaggccca atgaggaaag gtgattggcc ggaactgtcc
    11341 gctacccgac cgggattaat ccggggtgat tgccggtgat gctacggcta ccattcgatg
    11401 tgacgacaaa ggtattctct gttcccacca taccaactcc cttgatccct ttctttggtt
    11461 ctaacgagat caccatacct tcctcgagag ggtggtcaaa tccttttgct atcactggat
    11521 gttcggcgat ctcaagacca atcccgtgac ctaaaaactg gaccttcctg tccccatatc
    11581 ccataaaatt atcgaggaac gaaggttcga gttctcccat gacactctca tagatctgtg
    11641 agggaatagc cccgggtttc aggtgtgagg cgaccgtatc ctgaatcttt acgcaccggc
    11701 ggtgaatttc gacaacttct tttggaaggc tccgtccaaa gacgtaggtc atggtcttat
    11761 cggtgtggta cccgtgaaat ccgcacccga tgtcgacaaa gaccaggtcc ccgtcatgca
    11821 ggttcctgtc acgactcccg agcaacggga cggcgggtga cagccccgca ttgccaccag
    11881 ggctgtcaag gtaggtggga tagatggagc tctcaccgaa tccgaccagt ccgagcatga
    11941 tgtctgattc agacatcccg aacctcacga tcccatgatg gccctcttcc atcataaggc
    12001 gatagagttc gttggtgaac tctgcctcgc tgatcccctc agtcagggct cccggaacca
    12061 gctcctcgag gactatgcgg tggatctctc ctgcccgttc catcaatcca cgttcatagg
    12121 ggctcttcac ggaacggatc tgcaggatct gcttgtcaac cgccctgacg tatttgaagg
    12181 ggaaatattt tcgcagcctt gagaagaggg cgagggggac ctgctcggtc tcgaggtaga
    12241 cagtatcagg aatcgatggt attgattgca ctgcatcacg gtagcttcgc atcggtttga
    12301 tctgcgggaa gagggattca tccatagccc gttcataact cctcttcacc cagaggcagg
    12361 cttctccgtc ccggggtatg aggagcatgc cgtcttgtat ggtaccgcag aaatagtaga
    12421 ggttgacctt gctgaaaacc gtggcaatct cccagtcggg gtgtatggtg tccatctgga
    12481 cccgaaaccg tttcatccgg ttgtcaagtt catcccgagg gacccttgta ttcatgaact
    12541 gtataattgg gagacatact atttactgat aatgaaaagg gtcatttaat gcacccattg
    12601 gaatgacgta tttttccttt cggaaggagt gcataccatc cgatatgacc aaatctctcc
    12661 tgaccggata ccatggggac ttgtcaaaaa gaagggggaa gatgcgccga ccgggactcg
    12721 aacccgggtt tagg
//
LOCUS       DAOOCO010000037        12388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4751_length_12388_cov_5.047028, whole genome shotgun sequence.
ACCESSION   DAOOCO010000037 DAOOCO010000000
VERSION     DAOOCO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4751_length_12388_cov_5.047028"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1791
                     /gene="acsB"
                     /locus_tag="PK154_03480"
     CDS             118..1791
                     /gene="acsB"
                     /locus_tag="PK154_03480"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HPW10157.1"
                     /translation="MTGNGETTELQEKYLLTFLSGDIITSRVSSGVKVGAEYRLVPLG
                     SRPFSAIHFIDIIARVAMMFGGVTPGDAGRLLAYAKERAKAIAIVFPGLDDEEIALVD
                     AFRLLGIPVIAAGDYESDEWPRVQPADAVRTGMDLKGIKVSVTAIPIPMACSPAFEGK
                     SIRREEMQVEFGGGRSPAFELLRVRPPAEVIDGKVTVIGPEIDAIPEGSAAPLGILIE
                     VAGKQMKKEYEPVLERRIHNFVNYGEGSWHVAQRDLIWVRLSKEAVARGVRIKDLGTL
                     LAAKFRMDFPDLLDAVQVTLITDEKTVLAERANAGKVYQERDDRILGMRDTDVDCFYS
                     CTLCQTFAPNHVCIITPERPALCGAITWLDGKIAHEISPAGANQPVEKGRIIDMERGE
                     FEGVNRFVKKASHGEVDRCSLYSIMEYPMTCCGCFECIAVMLPEVNGFMVVNREYKGM
                     TPSGMSFSTLAGTIGGGAQTPGFAGISKGYILSDRFLQAEGGIERLVWMPEILKQELK
                     GRLLEYLKKRGIETFFNTIADETRAETIEDLAAFLASVDHPALAMKPLL"
     gene            1798..2754
                     /locus_tag="PK154_03485"
     CDS             1798..2754
                     /locus_tag="PK154_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HPW10158.1"
                     /translation="MMGVSTLSEWTGRIGELVIGATAKEGGTRSHAVTLGGEDDLPYI
                     GNPPSGHRPLVAYELSDDPELWPAAATRFIGDRVHDTAEWAAHAEKEYRPDLIRLYLS
                     STRERNFSDISRIGETVESVLQATELPLIIEGSNDPAIDSAVFQKIGEAAEGERILLG
                     TAEAGHYRSVAAAAMAYGHSVIAQSPIDINLAKQLNILLRETGLSPDRIAIDPYTGAL
                     GYGFEYSYSVMERIRYSALKGDSDLAMPMISSAIDSLVVKEVREADEGDACQTAIAWE
                     IYAMLPATIAGASIVCVRHPLSIEPLKTAIAALWNGEPKGGM"
     gene            2759..4093
                     /gene="acsC"
                     /locus_tag="PK154_03490"
     CDS             2759..4093
                     /gene="acsC"
                     /locus_tag="PK154_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HPW10159.1"
                     /translation="MALKPLEIYKLLPKKNCKECGDPTCLTFAMKLAGGKAKPEACPY
                     LDENAKTILGASTRPPVRKIRIGVGERAVTIGEELVQFRHEKSFFHEPGIFMAVTDLM
                     TPGAIQERVIFVRDEILTRVGTDLRLNGIAVRSVNGSPTAFTKAVEAVEAVADLPEVL
                     MAGDPAVMEAGLSVCGNYRPLLHAATSSQYGEMCRLAKKYACPLAIRVDSLDALAELS
                     GLCSAEGIENLVLDLSSPSLNEQLNRSTAVRQHAVSRKTPALGYPVCIDTAVSGLPDA
                     ALIAGVTKFGSVLICDDLSSASRKALLVLRQNIYTDPQKPIQITPGLYRVGSPGKDAP
                     VLLTVNFSLTFFTLQGYLEASRVPCFMLIVDTEGLSVLTAVASGKLQEMLVRDAIAKY
                     NLSAEVAHKRLIIPGYAAPLSGRIEDATGWKVLVGPRDAADIAEFLEKEWTA"
     gene            4096..6003
                     /locus_tag="PK154_03495"
     CDS             4096..6003
                     /locus_tag="PK154_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPW10160.1"
                     /translation="MPTVTFLPSFRKTEVPKGSTILEAAQKAGFHMNVVCGGQGKCGK
                     CSVMVRSGEIDFDREKYGSFYTDEEASQGFCLSCQARINGDLRVLIPEGSLVQEQQIL
                     VDSPGVPLEINPAVWKYALTLSPPTLDDQSPDLTRLLWGIEKSGGPKEGSIYAPLEVI
                     REIPKVLRESGWRITATVALVPGGYRMINVEKGDTTSHSYGAAVDLGTTTVVVYIRSL
                     IDGSIVGTGSNYNRQISCGEDILSRVNYAKRGGLEKLQALAVESINAALTAASNSAGI
                     DRDDIYEVVVAGNTIMTHILMGIDPAYMIEEPYVPVVRRYLTTAARRVGLAVGKNSGL
                     FIFPAVSDFIGGDIIADILASGMAESNDISLLIDIGTNFEVVLGNRDWMFACAGAAGP
                     ALEGGEVLFGMRANPGAIEQVSLDLRTLKPSFRTIRNQQPRGLCGSGLIDLLAELLRS
                     CIIDRTGRINTAIAHERIRQGRQVPEFVVAWKDETAIGKDIVITENDIKALIMSKASI
                     LAACQTLMNQAGIGREEIARIYFSGAFGNYINKDHAITIGLIPEIPVERVTTIGNGAI
                     AGANIALLNRRKKRVIDEIARKIAYIELNADPTFMDEYTGSCFLPHTDLSLFPGVEKM
                     LDQCRILREQS"
     gene            6000..7037
                     /locus_tag="PK154_03500"
     CDS             6000..7037
                     /locus_tag="PK154_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10161.1"
                     /translation="MSSWIAPPDSLATGVGALPHTDPREACDAVLALFPEFPYIPTLP
                     NHGLLESIVFCDSGRLPGGVIREGRLGIDQARDIPGEMEQIYLDFIEKNTAPYALTPE
                     YASGFAEMSGRDFSGSRILKCQVTGPVTFGMQVVDTERRPLAYDDQYADILPKMIALR
                     ARWCEEAMARFSGVKQTLVVVNEPYFASLGSTVVPVQQETVSAGWQDIAGMVEGGLGI
                     HCCSNTDWAYVISLDPAFLSFDAYTGIREFLLFMEDIVAYLERGGVVAWGLVPAQVQE
                     FRAVTQAELYRRFLEIREQITEFCSPDLFFRQSVITPTCGIQAGDTAASVEIMRAAAA
                     LSEQIREEWSG"
     gene            7034..7801
                     /locus_tag="PK154_03505"
     CDS             7034..7801
                     /locus_tag="PK154_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW10162.1"
                     /translation="MRPFSIALSGKGGTGKTTLSALLVRALVDAGRTPVLAVDADPNA
                     NLHEALGVQLHETLGCMRETEFRRNIPAGMARNDYIELCFRRALVEEKGFDLVAMGRP
                     EGSGCYCFANDLLKTCMTLLRRDYRFIVIDNEAGMEHLSRGTIGQPDIIVIVSDPGTR
                     GMRTAVRIRDMARSLGLSPVPVFLVINRFKGTDASVIAWEPFALIPEDPEIEAAEISG
                     LPVGSVKEGSVARKAVSRLAEQIIAMAEERAGNRSDR"
     gene            complement(7809..8030)
                     /locus_tag="PK154_03510"
     CDS             complement(7809..8030)
                     /locus_tag="PK154_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10163.1"
                     /translation="MRIRMLIAVVVVIILLLIVIALVAGALLLSTGYPPGGENSGLSG
                     MVSSLESLFGEISTGIADWMDYFVNLLAG"
     gene            8181..9680
                     /locus_tag="PK154_03515"
     CDS             8181..9680
                     /locus_tag="PK154_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HPW10164.1"
                     /translation="MCARFLDRFFKPRPHIVESPPPPSISHGPGIYVPEFKVKPYFIV
                     ASVEMGNTTTKCILTGVNLETGMSYVINKTVKMSRDVRKPKPGEEIFGETLDGTQLTK
                     ESVTDLVRDTLIQCHREVHLDIQKDLDFVVRSTGVVAAMDSPDQVGTFVLALANGCLN
                     AGVPPRKMTPPMAKANQPQKLQPYSYADKVVFIGAVAGVIPPVGSTGGEMVANEMEGE
                     LAMAGIKEGAMWTPVDFRNPCISIDFGTTLDGRITSDVAKDDPNPFAKTIGNFCGLAG
                     AIPDAIVKGSGLVDPKTGTALDVFGDRSKIAEFSLKGQGDKVMGYVKKCHEFIDIRIV
                     PPERRRFGRVPVYADVAKDSGVALIGCDAGENGDALGDLQAIGAEIYRNYSLSFLNDV
                     IDHVCAEMALRLVDVAQEEGLVLPNSSIGFTGRAAISGRKPEYILEGITERKLFTDPN
                     DHLVFVDDGLARGAALMGRCMNSLGKPKNPIGGVRGGPCIMARRIKAGK"
     gene            9714..9965
                     /locus_tag="PK154_03520"
     CDS             9714..9965
                     /locus_tag="PK154_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10165.1"
                     /translation="MDATGEEAQTELFDLLIPPGVPSKLIIEIAKKFDVEVVERKERL
                     YFANMEGDERELLAFRGKRENLEEAHAYLVEKLKEFIGE"
     gene            complement(10087..11037)
                     /locus_tag="PK154_03525"
     CDS             complement(10087..11037)
                     /locus_tag="PK154_03525"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10166.1"
                     /translation="MAAVFGGEPDIDAMREEKDFRGLITALRHDKLDIQWHASSALAS
                     LGDEAIDHLLSALNARNKDVRLGIIEALGEIRSPRAVGPLIGLLQDKDNEVRWEAALA
                     LGEMADPRALGPLEDALRDPDRYVRYGSALALEKMGWSPDTPERFAFLLAGKQEWDAL
                     AEMGRNAVSSLDIAAKDNDSSVRLMAVRTLGRIGDEKAIPLLYRALRDPDEKVRWEAV
                     MASQKCGISAQYLPRALARRPRTRKNPNIAAFLNFVLPGIGYFYIGKWWGVLIFQIDT
                     YATLLLSTELGLLNALDYLLPLYGIAALHAWYIARQMPDL"
     gene            complement(11115..12077)
                     /locus_tag="PK154_03530"
     CDS             complement(11115..12077)
                     /locus_tag="PK154_03530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10167.1"
                     /translation="MPRLLFRPGRLPDVRELEQKGDTDRLISLLNHHDFDVQTQAAAA
                     LERTKGDLVIGKLINALDTPDRKMRIGIVEILSNLHDPRAVPGLLTLLSRDESNEVRW
                     ASAIALGEIGDPAARDGLVTALSEPDKYVRYGAALSLEKNGWTPPDPEGRVYFLAAAQ
                     RWADIPGPDMVPVRPLIHHLSDADPAVRANVVKILGELHDPAARAACDLVLRDITSTV
                     RKEALRAFPKCNIPLMHLPRGISKRRRQRSAFAAMVLNFFCIGLGYNYLGRWWGFLVF
                     QVNVTAILTMSLFVPSYVSYLTSWAISAPFALHTYTYVKGMPDL"
     gene            complement(12040..12180)
                     /locus_tag="PK154_03535"
     CDS             complement(12040..12180)
                     /locus_tag="PK154_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10168.1"
                     /translation="MRAAPGILIVTTASRTGENSCERETTPAGDRSGMNAPSSLPPRQ
                     IT"
BASE COUNT         2779 a         3446 c         3655 g         2508 t
ORIGIN      
        1 cggcttcgtg ttctgcacgt ccaactgcgt gttccgtggg atgccgctgg agagctacga
       61 ccttattcat aatctctggc tggagcatcg ttactattcc agtgaggaac gctccgcatg
      121 acgggcaatg gagagaccac cgaactccag gagaagtacc tgctcacgtt cctctcagga
      181 gatatcatca cgagtcgcgt ctcctcggga gtgaaggtgg gggctgaata ccgccttgtg
      241 ccactcgggt cccgtccctt ctccgccatc catttcatcg acatcatcgc ccgggttgcc
      301 atgatgtttg gaggggtcac cccgggggat gcgggacggc tgctcgccta tgcaaaggag
      361 cgggcaaagg ctattgcaat cgtgtttccg ggcctggatg acgaagagat tgccctcgtc
      421 gatgcattcc gcctccttgg aatcccggtc attgctgcag gagactatga gtctgacgag
      481 tggccccgtg tccagcctgc tgacgcggtc cggaccggga tggatctgaa agggatcaag
      541 gtgagcgtga ccgccatccc gatccccatg gcctgttcac ctgcattcga aggaaagagc
      601 atccggagag aggagatgca ggtcgagttt ggcgggggaa ggtccccggc attcgaactc
      661 ctcagggtcc ggcccccggc cgaggtgata gatgggaagg tcacggtcat cggccccgag
      721 atcgatgcaa tccctgaggg atcggcagca ccgcttggga tcctcatcga ggtagcagga
      781 aagcagatga agaaagagta cgagccggtg cttgaacgac ggatccacaa tttcgtaaat
      841 tacggggaag gctcctggca tgtcgcccag cgggacctga tctgggtccg cctctcaaaa
      901 gaggccgttg ccaggggagt ccggataaaa gacctcggga ctctgcttgc ggcaaaattc
      961 aggatggact tcccggacct gctcgatgcc gtccaggtca cgctcatcac cgatgaaaaa
     1021 accgtgcttg cagaacgtgc caatgccggg aaggtctacc aggaacggga tgaccggatc
     1081 ctcgggatgc gggataccga tgtcgattgc ttctactcct gtaccctctg ccagaccttc
     1141 gcaccaaacc acgtctgcat catcaccccc gaacgacctg ccctctgcgg cgccatcacc
     1201 tggctggacg gaaagatcgc acacgagatc tcccctgcag gagccaacca gccggtggag
     1261 aaggggagga tcattgacat ggaacggggt gaattcgagg gggtcaaccg gttcgtcaag
     1321 aaagcctccc atggcgaggt agaccggtgc tcgctctaca gtatcatgga gtatccgatg
     1381 acctgttgcg ggtgctttga atgcatcgcg gtcatgctcc ccgaggtgaa cggattcatg
     1441 gtggtgaacc gggaatacaa ggggatgact ccctcgggga tgagcttctc cacccttgca
     1501 ggtaccatcg gggggggtgc ccagaccccg ggctttgccg ggatctcgaa ggggtatatc
     1561 ctctcggacc ggttcctcca ggctgaaggc gggatcgagc ggctggtctg gatgcctgag
     1621 atcctgaagc aggaactgaa gggcaggctt cttgaatacc tgaaaaagag gggcattgaa
     1681 acgtttttca acacgatcgc tgatgagacc agggcagaga cgatcgaaga cctggcagcc
     1741 ttcctcgcat cggttgacca cccggcgctc gccatgaaac cattgttgta ggtggagatg
     1801 atgggagtat caacactatc tgaatggacc ggccggatcg gcgaactggt gatcggtgcg
     1861 acggcaaagg aaggcgggac caggagccat gcagtgaccc ttgggggaga agacgatctc
     1921 ccgtatatcg gcaacccccc ttctggccac agaccacttg ttgcctacga actctcggac
     1981 gatcctgaac tctggccggc tgctgccacc cggttcatag gcgaccgggt ccatgatacg
     2041 gccgagtggg ccgcccatgc tgagaaggag taccgacctg acctgatccg gctgtacctg
     2101 tcgagcaccc gggaacgaaa tttctctgat atctcccgga taggggagac ggttgaatcc
     2161 gtgctccagg caacagagct cccgctcatt atcgagggca gcaacgatcc ggccatcgac
     2221 agtgccgttt tccagaagat tggtgaggcg gcagaaggcg aacggatcct cctcgggacg
     2281 gcagaggcag ggcactaccg gagtgttgcc gccgctgcca tggcatacgg tcattcggtc
     2341 atcgcccagt cacccatcga tatcaacctg gcaaagcagc tgaatatcct ccttcgtgag
     2401 acagggctgt cccctgaccg gatcgccatc gatccttaca ccggggcact gggatatggg
     2461 tttgaatatt cctactcggt catggagcgg atccggtatt ctgccctcaa gggcgacagc
     2521 gatcttgcca tgccgatgat cagctcggcg atcgattccc tggtcgtaaa agaagttcgt
     2581 gaggcggatg agggagatgc ctgccagact gccatagcct gggagatcta tgccatgctc
     2641 ccggcaacca ttgcaggggc ttccattgtc tgcgtgcggc acccgttgtc gatagagccc
     2701 ctcaaaaccg caattgcagc actctggaac ggcgaaccaa aaggaggtat gtgatcatat
     2761 ggccctgaaa ccgctggaga tctacaagct cctgcccaaa aagaactgta aagaatgcgg
     2821 cgaccccacc tgcctcacct tcgcgatgaa gcttgccggc ggaaaggcaa aaccggaagc
     2881 atgcccatac ctcgatgaga acgcaaaaac catcctcggg gcttcaaccc gtcccccggt
     2941 caggaagatc cggatcggag taggagaacg ggcggttacc atcggcgaag aactggtcca
     3001 gttcaggcac gagaaatcct tctttcatga accgggaatc tttatggcgg tcaccgacct
     3061 gatgaccccg ggggcaatcc aggagagagt catctttgtc cgggacgaga tcctgacaag
     3121 ggtcggcacc gacctcaggc tcaacgggat tgcggtacgg tcggtgaacg gttcaccgac
     3181 tgcgtttacg aaggcagtcg aggcggtcga ggcggtcgcc gacctgcccg aagtgctgat
     3241 ggctggagat ccggcggtga tggaagcagg actttccgta tgcggcaatt accggcccct
     3301 tctgcatgct gccacatcct cacagtacgg ggagatgtgc cggctcgcaa aaaaatacgc
     3361 ctgtcccctt gccatcaggg tggattcgct cgatgcactt gcagaactgt caggcctctg
     3421 cagtgccgag gggatcgaga acctcgtcct tgacctctca tctccctcgc tcaacgagca
     3481 gctgaaccga tctaccgcgg tgaggcagca tgccgtcagc cggaagaccc cggcactcgg
     3541 gtatccggtc tgtatcgaca ccgcagtgag cggtcttccg gatgctgccc tgattgccgg
     3601 agtcaccaag ttcggatcgg tgctgatctg cgatgacctt tccagcgctt cgagaaaagc
     3661 attgctggta ctccggcaga acatctatac tgatccccag aaaccgatcc agatcacccc
     3721 cggactgtac cgggtcggga gcccgggaaa ggatgctcct gtactcctga ccgtcaattt
     3781 ctcactgacc tttttcaccc tgcagggata cctcgaagca tcacgcgttc cctgcttcat
     3841 gctgattgtc gacacggaag gcctctctgt actcacagcg gtggcttccg ggaaactcca
     3901 ggagatgctt gtccgggatg cgatcgcaaa atacaacctt tctgccgaag tagcgcacaa
     3961 gaggcttatc atccccgggt acgctgcacc gctctcgggg aggatcgagg atgcaacggg
     4021 atggaaggtc cttgtcgggc cccgtgacgc tgcagacatt gccgaattcc tcgagaagga
     4081 gtggacggcc tgaccatgcc gaccgtgaca tttctcccga gcttcaggaa gaccgaggta
     4141 ccgaaaggga gcaccatcct tgaggcagca cagaaggccg gctttcacat gaacgtcgtc
     4201 tgcggggggc aggggaaatg cgggaaatgc agcgtcatgg tcaggagcgg cgagatcgat
     4261 ttcgaccggg agaaatacgg gagtttctat acggatgagg aagcatccca gggattctgc
     4321 ctctcgtgcc aggcaaggat caacggagac ctgcgggttc tcatccctga aggatccctc
     4381 gtccaggagc agcagatcct ggtcgattcg ccgggcgttc cccttgaaat caatcctgct
     4441 gtctggaaat atgccctcac cctcagccct cccacccttg acgaccagtc accggacctc
     4501 acccgcctgc tctgggggat tgagaagagc ggcggcccga aagagggatc catctatgcc
     4561 cctcttgagg tgatccggga gataccgaag gtgctccggg agagcggctg gaggatcacc
     4621 gcaaccgttg ccctggtgcc cgggggatac cggatgatca acgttgagaa aggagacacc
     4681 acatcccaca gctacggggc agcagtcgac cttggaacaa cgaccgtcgt ggtatatatc
     4741 cggtccctga ttgacggcag tatcgtgggg accggttcca attacaaccg tcagatcagc
     4801 tgcggcgagg atatcctctc ccgggtcaac tatgcaaaac ggggagggct cgagaaactc
     4861 caggcacttg ctgtcgaaag tatcaacgct gccctcaccg cagcatcgaa ttcggccggg
     4921 atcgaccgtg acgatatcta tgaagtggtg gttgcaggaa atacaatcat gacccatatc
     4981 ctcatgggga tcgatcccgc gtatatgatc gaggagccct acgtcccggt ggtgaggcga
     5041 tacctgacaa cggctgcccg aagggtcggg cttgccgtgg gcaagaactc gggcctcttc
     5101 atcttcccgg cggtgagcga tttcatcggc ggggacatca tcgccgatat cctcgcatcg
     5161 gggatggcag agtcgaatga tatttcgctc ctgatcgata tcggtaccaa ctttgaggtc
     5221 gtgctcggca accgggactg gatgttcgcc tgcgccgggg ctgccggacc ggcgcttgaa
     5281 gggggtgaag tactgttcgg gatgagagcc aacccgggtg cgatcgagca ggtgagcctc
     5341 gacctccgga ccctgaaacc ctcctttcgc accatcagga accagcaacc gagggggttg
     5401 tgcgggtcag ggctgatcga tctcctcgcc gagctgctca gatcctgtat catcgatcgg
     5461 accggacgga tcaataccgc catcgcccat gaacggatca ggcagggccg ccaggtaccc
     5521 gaattcgtcg ttgcctggaa ggacgagact gcgataggaa aggatatcgt catcacggag
     5581 aatgatatca aagccctgat catgagcaag gcatccatcc tcgctgcctg ccagaccctg
     5641 atgaaccagg ccgggatcgg acgggaggag atcgcccgga tttacttctc cggggcattt
     5701 ggtaattata tcaacaagga ccatgccatc accattggcc tgatccccga gatcccggtg
     5761 gaacgggtga ccacgatagg gaacggggcg atagccggcg ccaatattgc cctcctgaac
     5821 cgcaggaaga agagggtgat cgatgagatc gcccggaaga tcgcctatat cgagctgaat
     5881 gccgatccca ccttcatgga tgaatacacc gggagctgtt ttctgcccca caccgatctc
     5941 tccctcttcc ccggcgtgga aaagatgctc gaccagtgcc ggatcctgcg ggagcagtca
     6001 tgagcagctg gatcgctccc cctgatagcc tggcaaccgg ggtaggagcc ctcccgcata
     6061 ccgatccgcg ggaagcctgc gatgccgtcc tcgcactatt ccccgaattt ccctacattc
     6121 ctacgcttcc caaccatggg ctgctcgaat ccatcgtctt ctgtgattca gggagacttc
     6181 cggggggagt gatcagggag ggacgactcg gtatcgacca ggcaagagac atcccgggag
     6241 agatggagca gatctacctt gatttcatcg aaaaaaatac cgctccctac gccctgacac
     6301 cggaatatgc atccgggttt gcagagatgt ctggccggga cttttcagga tcacggatcc
     6361 tcaagtgcca ggtcaccggc ccggtcacct tcgggatgca ggtcgtggac acagaacgcc
     6421 gtcccctcgc ctatgatgac cagtatgcgg atatcctccc aaagatgatc gcactccggg
     6481 cccgctggtg cgaagaggcg atggccaggt tttccggggt gaaacagacc ctggtggtag
     6541 tgaacgaacc ctacttcgca tcgcttggtt ccaccgtcgt tcccgtacag caggagacgg
     6601 tcagcgccgg gtggcaggat atcgccggga tggtagaagg aggtcttggg atccactgtt
     6661 gttcgaacac ggactgggct tacgtgatca gcctggaccc cgcgttcctc tcgtttgatg
     6721 catacacagg catccgtgag ttcctcctgt tcatggagga tatcgttgcc tacctggaac
     6781 ggggaggtgt ggtcgcttgg ggactcgtcc ctgcacaggt acaggagttc agggctgtca
     6841 cccaggcaga attgtaccgg cggttcttgg agatcaggga acagatcacc gaattctgtt
     6901 cccccgacct gttcttccgg caatcggtca tcacgccgac ctgcggcatc caggccggtg
     6961 ataccgccgc atcagtcgaa atcatgagag cggctgctgc tctgtcggaa cagatcaggg
     7021 aggagtggtc agggtgagac ctttttccat cgcactatca gggaaaggag gtacaggaaa
     7081 gaccaccctt tctgcactcc tggtgcgggc actcgtagat gccggcagga cccccgtgct
     7141 tgcggttgat gccgatccaa acgccaatct ccatgaagca ctcggcgtcc agctccacga
     7201 aaccctcggg tgcatgcggg agaccgaatt cagaaggaac attcctgccg ggatggcacg
     7261 aaacgattac atcgaactct gtttcaggag agcgcttgtt gaggagaagg gatttgatct
     7321 ggtcgcgatg gggaggcctg aaggatcggg ctgctactgc tttgcaaacg atctcctgaa
     7381 gacctgcatg acccttctcc gcagggatta ccgcttcatc gtgatcgaca acgaggcagg
     7441 gatggagcac ctgagcaggg ggaccatcgg gcagcctgat atcattgtca tcgtgagtga
     7501 tcctggcacc cgggggatgc gaaccgcggt gcgtatccgt gacatggctc ggtcgcttgg
     7561 cctctccccg gttccggtat tccttgtgat caatcgtttc aagggaactg atgcctcggt
     7621 cattgcctgg gaaccgttcg ctctcatccc cgaagacccg gagatcgagg ccgcggagat
     7681 atccggattg ccagtaggat cggtcaagga aggatcagtg gcacgaaagg ccgtatcccg
     7741 gctggcagaa cagatcatcg cgatggcaga agagagagca gggaaccgat ccgacaggtg
     7801 agactgcttc atcctgccag caggttcacg aagtaatcca tccagtctgc aatcccggta
     7861 ctgatctctc cgaacaggga ttcaagagaa gagaccatac cggagagtcc ggaattctcc
     7921 ccgccgggag gataccccgt cgagaggagc agggctccgg caacgagggc aataacgatg
     7981 agaagcagta tgatcaccac gacgaccgct atgagcatcc ggattctcat ggccttctct
     8041 tccttattgg gtgcatcagt attaaaaata agcacgggca ccggacctgc cggatcctga
     8101 tactcgtcgc tcccgcgtaa ttccgacgat acgctattaa aatcgaaaga tataagggag
     8161 cagagtctta catcgtgtat atgtgtgccc gttttttgga tcgttttttt aaaccccgcc
     8221 cacatatcgt ggagagcccc cctcctccgt caatcagcca tggtccgggc atttatgttc
     8281 ccgagttcaa ggtgaagcct tattttattg tggcatctgt cgaaatgggt aacacaacga
     8341 caaagtgcat tcttaccggg gtaaatcttg agaccggcat gagctacgtg atcaacaaga
     8401 ccgtgaagat gagccgggat gtgagaaaac ccaaaccagg tgaggagatc tttggggaga
     8461 ccctggatgg gacacaactc accaaggagt cggtcaccga tcttgtgagg gacaccctca
     8521 tccagtgcca ccgggaggtg cacctcgata tccagaaaga cctggatttc gtcgtccgca
     8581 gcaccggggt tgttgcagcg atggactctc ccgatcaggt ggggaccttc gtccttgctc
     8641 tcgccaatgg ctgcctcaat gcaggggtgc cgccaagaaa gatgaccccc cccatggcaa
     8701 aagccaacca gccccagaag ctccagccgt actcctatgc ggataaggtg gtctttatcg
     8761 gggctgtagc aggggtgata ccccccgtgg gcagtactgg tggcgagatg gtcgcaaacg
     8821 agatggaggg tgagcttgcc atggcaggga tcaaggaggg agcgatgtgg acaccggtgg
     8881 acttccggaa tccctgcatc tcgatcgact ttgggacgac cctggacggc cggatcacga
     8941 gcgatgtggc aaaggacgat cccaacccgt ttgccaagac gatcgggaac ttctgcggcc
     9001 tggcaggggc gatcccggat gcgatagtga aaggatccgg gctggtggat ccaaagaccg
     9061 gaacagccct tgatgtattc ggggaccgga gcaagattgc ggagttctcc ctgaaaggcc
     9121 agggcgataa ggtcatgggg tatgtcaaaa agtgccatga gttcatcgac atccggattg
     9181 ttcctcctga acggaggaga tttggcaggg ttcctgtcta tgccgacgtg gcaaaagact
     9241 ccggagttgc actcatcggg tgtgatgcag gcgagaatgg cgatgccctt ggtgatcttc
     9301 aggccatcgg cgctgagatc taccggaatt acagtctgag cttcctgaat gatgtgatcg
     9361 atcacgtctg cgctgagatg gccctgaggc ttgtggatgt ggcacaggaa gaggggctcg
     9421 tcctgccgaa ttcctccatc ggatttaccg gcagggcagc catatccggc aggaaacccg
     9481 aatacatcct tgaagggata acagagagaa aactcttcac cgatccaaat gaccacctgg
     9541 tctttgtcga tgacggtctt gcccggggag ctgccctgat ggggcgatgc atgaattcgc
     9601 tcggcaagcc caagaatcca atcggagggg tcaggggcgg cccctgtatc atggcaagac
     9661 ggataaaagc aggaaagtag aggtatcagg atatgtctga ggataattca caaatggacg
     9721 caactggaga agaggcacaa accgaactgt tcgatctcct gatccccccc ggcgtcccca
     9781 gcaagctgat catcgagatc gccaagaaat ttgacgttga agtggtggaa cggaaagagc
     9841 gcctttattt tgccaacatg gaaggagatg aacgggagct tcttgcgttt cgggggaaac
     9901 gcgaaaacct ggaagaggct catgcctatc tggttgaaaa attgaaagaa ttcattggtg
     9961 aatgaatcgc tggaaaagag ccactcccgt tttttcgggg cgattccctt cagggcaagg
    10021 gctcccttcc ctgttctctc tccgactgac cagaaagccc cggttatcag ccagtgaggg
    10081 atatactcaa aggtccggca tctgccgggc aatataccag gcatgcaacg ctgcgatacc
    10141 atagagtggc aggagataat caagggcgtt gagaagcccc agctcagttg acagcaataa
    10201 ggttgcatag gtatcgatct ggaagattaa cactccccac cacttcccga tgtaaaaata
    10261 acctatcccc gggaggacaa agttgaggaa tgccgcaatg ttcggattct tcctggtccg
    10321 tggccgccgg gcgagggccc ttgggaggta ctgggcagag ataccgcatt tctgcgacgc
    10381 catgactgct tcccaccgga ctttttcatc agggtcacgg agtgcacggt aaaggagggg
    10441 gatggccttc tcatctccga ttctgccaag ggtcctgacc gccatcagcc ggacagagct
    10501 gtcattgtct tttgcagcga tgtcaaggga ggataccgca ttcctcccca tctcggcaag
    10561 cgcatcccac tcctgttttc cggcgaggag gaaggcaaac cgctccgggg tgtccgggga
    10621 ccatcccatc ttttcgagcg ccagcgcaga tccataacgg acgtaccggt caggatcccg
    10681 caaggcatcc tcaagggggc caagagcccg gggatcggcc atctcaccaa gggcgagcgc
    10741 agcctcccac ctcacttcat tgtctttgtc ctgaagaagc ccgatcaggg gcccgacagc
    10801 ccggggactc ctgatctcgc ccaatgcttc gatgatcccg agacggacat ccttgttccg
    10861 ggcattcagg gctgacagga gatgatctat cgcttcatcc ccgagagagg caagagcact
    10921 cgaggcatgc cactggatat cgagtttgtc gtgcctgaga gccgtgatca gcccgcgaaa
    10981 atccttctcc tcccgcatgg catcgatgtc aggttctcct ccaaagactg ctgccatgta
    11041 ctaccccctc caaaagggcg atcgttcgca tgagatggag ggggaagcgg ggtttttcct
    11101 cagacgatca ccgctcataa atcaggcatc cccttcacat aggtataggt gtggagtgca
    11161 aaaggtgcag agatcgccca ggaggtgaga taggaaacgt aggagggaac gaacaggctc
    11221 atggtcagga tagccgtgac gttcacctgg aataccagga acccccacca ccttcccagg
    11281 tagttgtagc caagaccgat acagaaaaaa ttcaggacca tggccgcaaa cgcgctcctt
    11341 tgccttctcc tctttgaaat cccccgggga aggtgcatga gcgggatgtt gcatttcgga
    11401 aacgccctga gagcctcctt ccggacggta ctggtgatgt cacggaggac aaggtcacat
    11461 gctgccctgg cggcgggatc gtgcagctca ccgaggattt ttactacatt ggctcttaca
    11521 gcgggatcag cgtcactgag atgatggatg agcggcctga ccggaaccat atcagggccg
    11581 ggaatatccg cccatcgctg ggcggcagca aggaaataca cccttccctc aggatctgga
    11641 ggagtccagc cgttcttttc gagggagaga gcagcaccgt accggacata cttgtcaggt
    11701 tcgctcaggg cggtgaccaa cccgtccctg gcagcaggat caccgatctc tccgagtgcg
    11761 atggcagatg cccatctgac ttcattgctc tcatcgcgtg agagcagggt gagaagcccc
    11821 ggaacagccc gtggatcatg gaggttggag agaatttcga cgattccgat ccgcattttc
    11881 cggtcagggg tatcaagggc gttgatcagt ttcccaataa ccagatcacc cttggttcgt
    11941 tcaagagcag ctgctgcctg tgtctgcaca tcgaagtcat gatgattaag gagtgagatg
    12001 agacggtctg tgtccccctt ctgttcgagt tccctcacgt caggtaatct gccggggcgg
    12061 aagagaagac ggggcattca taccgctcct gtcccctgca ggggtcgtct ccctttcaca
    12121 gctgttctct cccgttctgc tcgcggtggt gacgatgaga atacctggag cagctctcat
    12181 tttaatcagt acttcggaaa aaagatgata tatctttgta tccgtgattt tccccggtgc
    12241 tgaggtgcca gtgtatcgtc atgggaggag aaacgtgagt gatccgagcc ctcgtacatg
    12301 tgagacgggg aaagacaaat ggcttgtaac ttccatccgg agatgcaggt tcggtgtaaa
    12361 aaagatgatc gcactcttac tgcttctt
//
LOCUS       DAOOCO010000038        12307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4793_length_12307_cov_4.838312, whole genome shotgun sequence.
ACCESSION   DAOOCO010000038 DAOOCO010000000
VERSION     DAOOCO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_12307_cov_4.838312"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK154_03540"
     CDS             complement(<1..707)
                     /locus_tag="PK154_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10169.1"
                     /translation="MAYFAKEMPLNPDQSLGPVIIGSKIRVENSEPLPLQEGRVEFVP
                     GQAWDPRHLSITKEMTPDRKKLNLHVGLAGTVPISVYTSLILRQQPITLRVTLIPAMA
                     MPGMGIPVAVPLVPVVDEVSVTIPQIPQPEMILDGTYVGKTGDGKDRAEVTATVMLPP
                     YISPYEHEELIRNISFEVTDVRNGRITLGGNSSDTMKKILELIAVPDPASPGGRITAT
                     IRSSISYNGLVLAGEKRL"
     gene            complement(726..1751)
                     /locus_tag="PK154_03545"
     CDS             complement(726..1751)
                     /locus_tag="PK154_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10170.1"
                     /translation="MRRNRSFPSSCTMGILVLLVLAGGIGLASGESLPTVMSINGPTI
                     DEFANSPGPFTFTATMSGGTPSYTYVWKKTSTPRMTADVFKCSQENSVTVPKDHFTMY
                     GGLYLEVTDAKGRMATWVNGDGQTMVVFSQYYEQLEQSVLPGCNVNRKIGDAWVMAKM
                     VNGACHSRTQLPALPATPSNTVIQNQPACAAPPEDSSWVVIVGGLAVVAVAAAAIARM
                     LKNRPKKKGEQPPDQYILQLSKDTLKVSLKKSDSFTVTAWKVTEDGGFVAVPTAQIRV
                     TPPRNIPELVIEPAAGNGSLAPSVSLRSFPGSVTGVITITASSGGQTVSADVKVTVEG
                     TPDIEFD"
     gene            complement(2113..2706)
                     /locus_tag="PK154_03550"
     CDS             complement(2113..2706)
                     /locus_tag="PK154_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW10171.1"
                     /translation="MPFCQDCGARLEEGSRFCTDCGAPVLDVPGSGPAVPADSDSGQG
                     PIGVEKVIAVIPNLMKVRGLFFKGPSWHHLVVTSERIIVVHKTVKGLSRIKQDIGVIG
                     PDYEYSFMKSMKPERIREENPESQVIPLGDLVSITVSKFVSYSTEDGTEAYWQVLLTT
                     KKEALNLRTDYHEDPGEYFRDPALMQLLGERLILQDI"
     gene            complement(2672..2845)
                     /locus_tag="PK154_03555"
     CDS             complement(2672..2845)
                     /locus_tag="PK154_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10172.1"
                     /translation="MPGIGDHNPLPIIVPVIADHHSVWEEKEQERQDYRSAGGDPRNG
                     DLHAILPGLRGKA"
     gene            2899..3483
                     /locus_tag="PK154_03560"
     CDS             2899..3483
                     /locus_tag="PK154_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW10173.1"
                     /translation="MRSFCTRCGAPIASGLRFCEQCGVPVAVSPSPHPRSPFIPLLEL
                     HEGAFNLKRTPAVMEINPGELRIFRLPEYFNREIEALRSRLDDAWMAVSDQNGDWKAK
                     ITSWNWNKEPLITALARNRAGLEAGGQDLLILEGAGIRSIRIEHVESDTVWEIMTIDN
                     SGRKVVIDITGPVAWHAFGLLSAVLGEKVSYEEE"
     gene            complement(3494..3934)
                     /locus_tag="PK154_03565"
     CDS             complement(3494..3934)
                     /locus_tag="PK154_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPW10174.1"
                     /translation="MAGPFCESCGAQLKEGDKFCEQCGAVTAAVSLAPASVAVPAAAV
                     QKEKNPWISTILSFFFPGLGSIYNGDTLKGVAIYIGAIIGMLLFIVPGVIVWIYGMYD
                     AYTTAKKMNEGLLPYKEANIMVIIGFIVAVLVIGAIALVFSMGL"
     gene            4062..4559
                     /locus_tag="PK154_03570"
     CDS             4062..4559
                     /locus_tag="PK154_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10175.1"
                     /translation="MKRVHLILLAFIIFSAGCVTTAEEWNQRGETHHTMGRYGEAVAA
                     FDQALSLDPGYSEAWKNRGLSLALLGRTQESGDSFSRAIALNPSDSEIFYYQALSRNS
                     SGNRSAAMEILDAAIALPPQNRDQAIILHQSLMMRGDLLTIENRTDEANRSYQRAHEV
                     MMGTI"
     gene            complement(4782..>5349)
                     /locus_tag="PK154_03575"
     CDS             complement(4782..>5349)
                     /locus_tag="PK154_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW10176.1"
                     /translation="GITHGIILSRPDRFFIPTGQVFSMPFCTSCGQELTPADRFCTSC
                     GAPAGPLTAPVLPVTQASPPPASQDEIRWTNKVLGMASDFFVNAEGAGQEASKKTSSV
                     NTGIAVVGGLLGSLSAAGGGILAKSREDDFIRWDESRSVALNRKKKTVTVTRKSLIFP
                     IRLYCTNENYEQVVAFIKKNVDPALIKE"
     assembly_gap    5350..5449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5450..5787)
                     /locus_tag="PK154_03580"
     CDS             complement(<5450..5787)
                     /locus_tag="PK154_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW10177.1"
                     /translation="MTGKENRCPKCGDSCSPGAQFCGSCGYRFDEKGTRDLARKVQET
                     VRSAESAARTAESAVRTATQVRDLVITPPAEWKVVVGDRLPEAVAAKATAAVQEKAAD
                     LVAEKAKEGL"
     gene            complement(5856..8012)
                     /locus_tag="PK154_03585"
     CDS             complement(5856..8012)
                     /locus_tag="PK154_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10178.1"
                     /translation="MGVSAMPETKILSLEKERERCIKVIQGCIPKDHRQALLDDLEKL
                     PYKGAMDYQVYAREVYETAWKIWAEDQKDYLWWENWGPSLFKGAEYAKMAGDLSFSLL
                     IGYATSSFGPAKSYGASTMASEFKDQGLEFYAYYVEHKAAKDFRTCLIDFVHERLTEF
                     LITLAQGAVDTVILQGIDIRNPKTYTRLAWLWLWKIEVNLARDPEAGLVKAMIAAGKE
                     VVTVAGMMLLQEFVNTHGNAKLREIHSSVKKKGLLGPGQSGGDEEGKQKPGEEDSNKK
                     QEKKPKDEAEGRKKEEKKPEEEAGKKKPDDPDAKKGDGKKSADKEQYEKRIEAERKKA
                     QKDADDAVERGKEFADQRARAAFEEGRAKGREKLDNLKVAASELQRNPNDPEAGKRFA
                     DACEEVQKDKHAMHELNDVDPNKPNKIREQFNEHWKGQYEKVDAQAKQRIADELNKNL
                     KPGEKPYTSDDIELAAVTNTPPKSPAEDSVKSTYDRDITYRDKRTGKDIPTKISEPIY
                     NNEFYKQQHGGKEPPSAEAAKEYAEQCDQTVTDRFHRDAYGGGKEDIGPAVDPNQKGK
                     PFKDVEASSKTMEDKVLEWYKRAGEATKASDAEACKEEGMRQLTKQFKNQIEGRINKV
                     NEIMGYPNPPSAEVPPKLRSAVDVMNRVGTKGPDGKTFTVADAEAALKQMGTSPRKVV
                     HEMAGVLESTQKHMSPKVREAVEQHVRDLEAADGGK"
     gene            complement(8003..10174)
                     /locus_tag="PK154_03590"
     CDS             complement(8003..10174)
                     /locus_tag="PK154_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10179.1"
                     /translation="MVALTFPYNPDSGRIWAVTVRSRIRIAGGEPVLLREPVETVIEP
                     GSGWEGLVTTSGHELMDGRRAARFEATISDGMPLSKFAGLVIPKIPVRIRVTLTPDLQ
                     APLPGVPVPVQAPGPTQTRVVSIAIPPIQPGMIELETFSLPDDDSGRPRGGVRARVRF
                     PSIVKPDTRELRMVQESITFVETGGKSAIDVMREYREGDDTKVREYAALASREVMDRA
                     QVMVEVTAVVCGLTIRASGQLSFAIRRTFQPYLVPRSLTVSPSSPASFTALVREMLPD
                     GQEVDVPGATISISVPSSAAGILSISPMNATGTLAATVTQGKDAALATFSCPVSFQVG
                     KDRYDDAVTVSVGQKAGPLEIEFVPPEKTAINPFIAADSVTIRARVSSGKAAITFARG
                     MTAQWLDEPSVGVSTGDGWVAVRVEASPPDPLSQAVPPESEDIVITAAENGGVIAEEA
                     VSIRIITPPPLDVSPAEVRLVPGKEEKAEVVLSLRGEATGKWRYDLEGDEDASRYLDI
                     STPMVEGSTARFQVTLIDEEPSDENDVGPSRWQQVYLFHSRASDGTTEIEGPDVKVLV
                     LREGLFVEKIFAVDEKNQYKTSDRMTILPIRVDLPTEDRKRTARVKLVAYVWNGTELV
                     QDEEAVRGDNLTWDPPVCTAGDCRKWESIFAVLKAALVITDRDEAALTAYSGLEGTWG
                     ISLDKVIPGHGEKLQGEITVHGDAGTVTIPIVLRLGEPSWE"
     gene            10426..11442
                     /locus_tag="PK154_03595"
     CDS             10426..11442
                     /locus_tag="PK154_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10180.1"
                     /translation="MSCRTWLFLLCVLLVITPANALCHWAPEVTPPPGVPPCSGSYDS
                     RQQPWPRLPTDTEIERAKEAYLAYQLEGSERRRIDSVVVAKYHTCEGTWINHVDMEVK
                     YSWWWESVTGEGEWQPSAAYTFLIYDPGSNRFEFVLDIGVSPYFMGLRTDAIQGFATG
                     PCSSGGGAVPTSSGGSAAPESDDNSWVVIIGAIAVIGGGLAIGAKKLAGGKGKQPSGE
                     KKKEKKEQEKVRYILQLSADRVTVTPAAPAHLTVTAWKIVGTSPPAPAPEASITLTVP
                     AGNEGLSVQPASGTGSVEAGIALVSQVAKSPVLITVTASAGKSSVSAQVTVEIPAEYL
                     MEFF"
     gene            11509..12171
                     /locus_tag="PK154_03600"
     CDS             11509..12171
                     /locus_tag="PK154_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10181.1"
                     /translation="MPYCETCGTELNEGTSCPSCVSGEGRTNAPSLEGLIWTAKAPVI
                     SSRPVVTQLILVFVIACLIVLAFLFLLDIDAGLGAAPYILGIMVFFIVLGLIIAAAIQ
                     FFTKGGPTTEFAVTPEGIGYRAGKESKTINRATLAGTALGGSLSGAGGSMINISREMD
                     FMAWDEVRSITVYPRERSLVFYRSMLIFPFALYCTPENFGQVKALAKKYAPDARYKEK
                     GW"
BASE COUNT         2424 a         3656 c         3411 g         2716 t
ORIGIN      
        1 agcctcttct caccagcaag gacgaggccg ttgtaggaga tcgatgaacg gatggtcgcc
       61 gttatacgtc cgccagggga ggccggatca gggacggcga tgagttcaag gatctttttc
      121 atcgtatctg atgaattgcc cccgagggtg atccgtccgt tcctgacatc agtcacttca
      181 aatgagatat tcctgataag ttcctcgtgc tcataggggg agatatacgg gggaagcatc
      241 accgttgcgg tgacctcggc acggtctttc ccgtcaccgg ttttccccac ataggtcccg
      301 tcaaggatca tctccggttg cggaatttgg gggatggtaa ccgagacttc atcgacaaca
      361 ggaaccagcg ggaccgcaac cgggatcccc attcccggca tggccattgc cgggatgagg
      421 gtgacccgga gtgtaatcgg ctgctgcctc aggatcaggg aagtatagac cgagatcgga
      481 acggtgcctg caagcccgac atggaggttc agcttcttcc ggtcaggggt catctccttt
      541 gtgatagaga gatgccgggg atcccatgcc tgtcctggga cgaattcgac cctcccctcc
      601 tgcaggggga ggggttcgct gttctcaacc cttatcttcg atccgattat caccggccca
      661 agcgactggt cggggttcag aggcatctct tttgcgaagt atgccatggc gttcgccccc
      721 tcccctcagt cgaattcgat atccggcgtt ccttcgaccg tcaccttcac gtcagcagag
      781 acggtctgcc ctcccgatga tgcggtaatg gtgataacac cggtaacgga ccccgggaag
      841 ctgcggaggg agactgatgg ggcaagcgac ccgttccctg ctgctggctc gatgacaagc
      901 tcagggatat tccgcggggg agtgaccctg atctgtgccg ttggaacggc aacaaaccct
      961 ccatcctcgg tgaccttcca tgccgttacc gtgaacgagt cgctcttctt caggctgacc
     1021 ttcagggtat ccttcgagag ctggaggata tactggtcgg gtggctgctc acccttcttc
     1081 ttcggcctgt tcttcagcat tcgggcaatc gccgctgctg ccacggcaac tactgcgagg
     1141 ccaccaacaa tgacaaccca ggagctgtcc tcaggaggtg ctgcacaggc aggctggttc
     1201 tggatgacag tgttcgaggg agtggcaggg agtgctggga gctgtgtccg cgaatgacag
     1261 gccccgttca ccatctttgc catgacccag gcatccccga tcttccggtt gacattgcag
     1321 cccgggagga cactctgttc aagctgctcg tagtactggc tgaacaccac cattgtctgc
     1381 ccgtccccgt tcacccatgt ggccatccgc ccctttgcat cggtcacttc gaggtataac
     1441 cctccataca tggtgaaatg atcttttgga accgtaacgg agttctcctg tgaacatttg
     1501 aatacatccg cagtcatgcg aggggtggag gtcttcttcc agacgtacgt ataggacggg
     1561 gtccctccgg acatggtagc cgtgaaggtg aacggcccgg gactgttcgc aaattcatcg
     1621 atggtgggac cattgatgct catcacggtg gggagacttt cgccggaggc gagcccgatt
     1681 ccgccagcaa ggaccaggag aacgagaatc cccatcgtgc atgatgaggg aaacgaacgg
     1741 ttccgtctca tgtgagattc tccatgaaga gaagtccatc ccgacttaaa acgggacatt
     1801 ctcgtgagtt cccggcatcc cgggactggt gaaagaagcg gacagtaccg cacaccagag
     1861 aatgtgtcgc gaggaatgca gtgtttccag atgaacaggt tgtgctgaaa tgaggtagaa
     1921 gaacggcagg tgaaattgga tccaacccct gtatagcagc ctttcatcgg gagatgatat
     1981 caagatttat attctgcatt atccatcgtg ccgcaacgca accctgcagt acatcataat
     2041 ccttcaaaat ccgtcaccgg gagatatcct gtgtcctgat tcggccgaac cggctattgt
     2101 tgaaagagag ggtcatatat cctgcaggat cagtctttct ccgaggagtt gcatgagggc
     2161 aggatcccgg aaatattcac ccggatcctc gtgataatcg gtcctgaggt tgagtgcttc
     2221 ctttttcgtc gtgagaagga cctgccagta tgcttcggtc ccgtcttccg tgctgtaact
     2281 gacgaatttt gagaccgtga tacttaccag gtcgccgagc gggatcacct ggctctccgg
     2341 gttctcttct ctgatccgct ccggcttcat cgatttcatg aaggaatatt cgtaatccgg
     2401 ccctatcacc ccgatatcct gcttgatgcg tgaaagcccc ttcacggtct tatgtacaac
     2461 aatgatccgc tcggatgtca ccacgaggtg gtgccaggac ggtcccttga aaaagagccc
     2521 ccggactttc atgaggttcg ggatgaccgc gatcaccttc tccaccccga ttggaccctg
     2581 ccctgagtca ctgtcagccg gtactgccgg tccactcccg ggaacgtcca gtacaggtgc
     2641 tccgcaatcg gtgcagaacc tggatccttc ttcaagcctt gccccgcagt cctggcagaa
     2701 tggcatgcag atctccgttt cgtggatctc caccggccga gcgataatcc tgccgttcct
     2761 gctccttctc ctcccagact gaatggtggt cagcgataac agggacaatg atgggaaggg
     2821 gattgtgatc ccctatcccg ggcaaaacca ccgttcttat gtacccggag caccccagat
     2881 ctcccggtga gcatccgtat gcgatcgttc tgtacccgat gcggtgcccc gattgcctca
     2941 ggattgaggt tctgcgagca gtgcggagtc cccgtggcgg tatccccttc acctcatccc
     3001 cgcagcccgt tcatcccact cctcgagcta cacgaaggag cattcaacct taaaaggacc
     3061 ccggctgtga tggagattaa ccccggggaa ctgcgaatct tccggctccc ggaatacttc
     3121 aacagggaga ttgaggcctt gcggagccgc ctggacgatg catggatggc cgtttccgat
     3181 cagaacggcg attggaaagc aaagatcacc tcatggaact ggaacaaaga accgctcatc
     3241 acggctcttg caagaaaccg ggctggcctt gaagcaggag ggcaggatct tttgatcctt
     3301 gaaggagcag gaatccggtc gatccggatc gagcatgtcg aatcagacac cgtctgggag
     3361 attatgacca tcgataatag cggaagaaaa gtggtgatcg atatcactgg accggtcgcc
     3421 tggcatgcat tcgggctgct ctccgccgtg cttggggaga aggtgtccta cgaagaagaa
     3481 taaaaaagag gaatcagagg cccatgctga atacaagggc aattgcaccg atgacgagca
     3541 cggccacgat aaacccgata atgaccataa tgttcgcttc cttgtagggg agcagcccct
     3601 cattcatctt ctttgccgtc gtataggcat catacatacc ataaatccag acgatcactc
     3661 ccgggacaat gaaaaggagc atcccgatga ttgcaccaat ataaatcgcg acacctttca
     3721 acgtgtcacc attgtagatc gagccgagcc ccgggaagaa gaacgagagg atggtggata
     3781 tccagggatt cttctctttc tgtaccgcgg cggccggaac agcgacagat gccggtgcca
     3841 gcgatacggc tgcagtcact gccccgcact gctcacaaaa cttgtcgcct tccttgagtt
     3901 gagccccgca tgactcgcag aatggtcctg ccatgagtgt accatgcgga aaggaatcat
     3961 aaataggtta tttttttgga tccgtgttct ttgaggggat tgattacccg atcaccccat
     4021 caataagaat cctcttgagg gatccgggtc tatctcccat catgaaaagg gtccatctta
     4081 tcctgctcgc cttcatcatc ttctctgcag gctgtgttac taccgctgag gaatggaacc
     4141 agcgggggga gactcatcac accatggggc gatacggcga agcggtggcg gcctttgacc
     4201 aggctctctc gcttgacccg gggtatagcg aggcctggaa gaaccgggga ctttcgcttg
     4261 cacttcttgg caggacacag gaatcagggg attcgttttc ccgagcgatt gccctcaatc
     4321 cctctgatag cgagatcttc tactaccagg ccctctcccg gaacagttca gggaaccggt
     4381 cagcggcaat ggaaatcctc gatgcggcca tcgcccttcc gccacagaac cgggaccagg
     4441 ccatcatcct ccaccagtca ctcatgatga gaggagacct tctcaccatc gaaaaccgaa
     4501 cggatgaagc aaaccgatcc taccagaggg cccacgaagt catgatgggg acgatctgat
     4561 caggaacagg aacggacagg aagaacgaac gtggaaagga ggagagagac agtcaccgga
     4621 taccagtcct gaaaggtttt agggggaact ggcatactcc agaggaagag tcatctgtca
     4681 ccggcctttc atcccaaacg gtggatccgg aatgatcctg agcctctctc cacctttcag
     4741 gcaattcccg gacactgatc tcctctgttc cctgatttcc ttcattcctt tatcagggca
     4801 gggtcaacat tctttttgat gaatgcgacg acctgttcgt agttttcatt ggtgcagtaa
     4861 agccggatcg ggaagatcag ggacttccgg gtcacggtca cggtcttctt cttccggttg
     4921 agggcgacgc tccgtgactc atcccaacgg atgaaatcat cctccctgct ctttgcgaga
     4981 atcccgcccc cggccgctga gaggcttccg aggagacctc ccaccaccgc gataccggta
     5041 ttgacgctgc tcgttttttt ggaagcctcc tggcctgcgc cttccgcatt tacgaagaaa
     5101 tcggatgcca tgccaaggac tttgttggtc caccggatct cgtcctggct tgcagggggt
     5161 gggctggcct gcgtcactgg cagtaccggc gccgtcaacg gcccagccgg cgcaccgcac
     5221 gaggtgcaga agcggtcagc aggagtgagc tcctgcccgc aggaggtgca gaatggcatg
     5281 gagaagactt ggccggtcgg gataaaaaaa cgatcgggtc tggagagaat tatcccgtgg
     5341 gtgatgcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caaggccttc
     5461 tttggccttc tccgcaacca gatccgcagc cttttcctgg actgcggcgg ttgcctttgc
     5521 cgccaccgcc tcgggaagcc ggtccccgac caccaccttc cactctgccg gcggtgtgat
     5581 caccaggtcc cggacctggg ttgcggtcct gactgcagat tcagcggttc gtgcggctga
     5641 ctcggccgaa cggactgtct cctggacctt ccgtgcgagg tccctggtac ctttctcatc
     5701 gaagcggtac ccgcaggatc cgcagaactg tgcccccgga ctgcaggaat ccccgcactt
     5761 cgggcagcga ttttctttgc ctgtcatcgt ctctctcccc cacccaacgt ttcaccggtg
     5821 gttatcatgc tgtccatcac tctgaaacct cctttttatt tcccgccatc tgctgcctca
     5881 aggtcccgga catgctgttc gactgcctcc ctcactttgg gagacatatg cttctgggtg
     5941 gattcaagga ctcctgccat ttcatgcacc actttccgcg ggctcgtccc catctgcttc
     6001 agggctgcct ctgcatcagc aacggtaaac gtctttccgt ccggcccctt tgtcccgacc
     6061 cggttcatca catcgaccgc agacctgagc ttggggggga cctctgctga cgggggattg
     6121 ggatagccca tgatctcgtt caccttgttg atccggcctt cgatctggtt cttgaactgc
     6181 tttgtgagct gccgcatccc ctcctccttg caggcttcag catccgatgc cttcgtggcc
     6241 tcaccagccc gcttgtacca ttccaggacc ttgtcctcca tggtcttcga tgaggcttcg
     6301 acgtccttga acggcttccc cttctggttc ggatcgaccg cagggccgat atcctccttg
     6361 cctccgccat acgcatcacg gtggaaccgg tcggtcacgg tctggtcgca ctgctcggca
     6421 tattcctttg ccgcctcggc gcttggcggc tccttcccgc catgctgctg cttgtagaac
     6481 tcgttgttgt agatcggctc ggaaatcttt gtcgggatat ccttcccggt ccgcttgtcc
     6541 cggtaggtga tgtcccggtc gtaggtggac ttgacactgt cttccgcagg cgatttcggg
     6601 ggggtattgg tgacggccgc caactcgata tcatcggaag tgtagggctt ctcaccgggc
     6661 ttcaggttct tgttcagctc atcagcgatc cgctgtttgg cctgcgcatc gaccttctca
     6721 tactgccctt tccagtgctc gttgaactgt tcccggatct tgtttggctt gttggggtcg
     6781 acatcgttca gctcgtgcat cgcgtgcttg tccttctgga cctcctcgca ggcatcggca
     6841 aaccgcttcc ctgcctccgg atcgttcggg tttcgctgga gctctgatgc ggccaccttc
     6901 aggttatcca gcttctccct ccccttcgcc cgtccctctt caaacgcggc tcttgctctc
     6961 tggtcggcaa actccttccc gcgttccacg gcatcgtcgg catctttctg ggctttcttc
     7021 cgttctgcct cgatccgctt ctcatactgc tccttatcag ccgacttctt cccgtcaccc
     7081 ttcttcgcgt caggatcgtc gggcttcttc ttcccggcct cttcctccgg cttcttctct
     7141 tccttcttcc tcccttccgc ctcgtccttt ggtttcttct cctgcttctt attcgagtct
     7201 tcttccccgg gtttctgctt tccttcctca tcccctccgg actgtccggg gccaagcagc
     7261 cccttcttct tcactgatga gtggatctcg cggagcttcg cattcccgtg ggtgttgacg
     7321 aactcctgga ggagcatcat acctgccacg gtcactacct ccttcccggc agcgatcatc
     7381 gctttgacca ggccggcctc aggatcccgt gcgaggttta cctcaatctt ccagagccag
     7441 agccaggcga gccgagtata ggtctttgga ttccggatat cgatgccctg caggatcacc
     7501 gtgtcgaccg caccttgcgc aagggtaatg aggaactcgg ttaaccgctc gtgtacgaaa
     7561 tcgatgaggc aggtgcggaa gtcctttgcc gccttgtgct cgacatagta cgcgtagaat
     7621 tcgagcccct ggtccttgaa ctccgaagcc atcgtggaag ccccgtagga tttcgcaggc
     7681 ccaaacgagc tcgtggcata cccgatgagg aggctgaagg agaggtctcc tgccatcttc
     7741 gcatactcgg cacctttgaa cagggagggc ccccagttct cccaccacag gtaatccttc
     7801 tgatcttccg cccagatctt ccaggccgtc tcgtagacct cgcgggcata gacctggtag
     7861 tccattgctc ctttataagg gagcttctcg aggtcatcta ggagagcctg ccggtggtct
     7921 ttcgggatac atccctggat gactttgatg caccgttccc gctccttttc aagggaaagg
     7981 atctttgtct ccggcattgc actcactccc atgatggttc acccagccgg aggacgatcg
     8041 ggatcgtgac ggtcccggca tcgccatgta ccgtgatctc tccctggagt ttctcgccgt
     8101 gaccggggat gaccttgtca agcgagatcc cccaggtccc ctcaagacca ctgtaggcgg
     8161 tgagggcagc ctcgtcgcgg tcggtgatga ccagtgcggc cttcaggaca gcaaagatgc
     8221 tctcccactt ccggcagtcc ccggccgtgc agaccggggg gtcccaggtg aggttgtccc
     8281 cccgtacggc ctcctcgtcc tgcacgagtt cggtcccgtt ccagacatac gcaaccagct
     8341 tcaccctggc ggtgcgtttc cggtcctcgg tcggaaggtc aaccctgatc gggaggatgg
     8401 tcatcctatc gctcgtcttg tactggttct tctcgtcaac ggcaaagatc ttctcgacaa
     8461 agagcccttc ccggaggacg agcaccttca catcgggccc ctcaatctcg gtcgtcccat
     8521 ccgaggcacg ggagtggaac aggtagacct gctgccagcg ggacggcccg acatcattct
     8581 catcggaagg ttcctcatca atgagggtga cctggaacct cgcggtcgat ccttcgacca
     8641 ttggtgtgct gatgtcaaga taccgcgagg cgtcctcatc tccttccaga tcgtatctcc
     8701 atttccctgt ggcttctccc ctcagggaga gtacgacctc cgctttctcc tcttttccgg
     8761 gaacgaggcg gacctcggcg ggggagacgt cgagcggggg gggggtgatg atgcggatgg
     8821 agaccgcctc ttctgctatc acccctccgt tctccgctgc cgtgatgacg atatcttcgg
     8881 attcgggagg taccgcctgg gacagaggat ccggaggaga tgcctcgacc cgcacggcaa
     8941 cccatccatc accggttgat accccgacac tcggttcatc cagccactgt gccgtcattc
     9001 cccgggcaaa cgtgatggct gcttttccgg aggaaacgcg ggcacggatc gtcaccgagt
     9061 ccgccgcaat gaacgggttg atggcagtct tttccggcgg aacgaactca atttcgagcg
     9121 gccctgcctt ctgcccgaca ctgactgtca cggcatcatc gtagcgatcc ttccccacct
     9181 ggaacgagac tgggcaggag aaggtcgcca gggcagcgtc ttttccctgg gtgacggtcg
     9241 cagcgagagt cccggtcgcg ttcataggac tgatggagag gatccctgct gccgaagagg
     9301 ggaccgagat cgagatggtc gccccaggaa catcgacctc ctgcccatca gggagcattt
     9361 cacggacaag ggcggtaaaa gaggcagggc tggaaggcga gacagtgagg gaacggggga
     9421 cgagataggg ctggaaggtc ctccggatag caaacgacag ctgcccggat gcccggatgg
     9481 tcagcccaca cacgacagcg gtgacctcga ccatcacctg ggcccggtcc atcacctcac
     9541 gggaggcgag tgccgcatat tcccgtactt tcgtgtcgtc cccctcgcgg tactcacgca
     9601 tgacatcaat agctgatttc ccacctgttt caacgaatgt aatcgactcc tgcaccatcc
     9661 tgagctcgcg ggtatccggt ttcacgattg atggaaaccg gacccgcgcc cgtaccccgc
     9721 cgcgtggacg cccagagtca tcgtcaggca gggagaaggt ctcgagctcg atcatccccg
     9781 gctggattgg agggatggcg atcgagacca cccgggtctg ggtgggacca ggggcttgca
     9841 ccggtaccgg gacccccggg aggggtgcct ggaggtcagg ggtcagcgtg acccggatcc
     9901 ggaccggtat tttcggaatg accagcccgg caaattttga aagcggcatc ccatcggaga
     9961 tagtcgcctc gaaccgggct gcccgcctcc catccatcag ctcgtgcccg gaggtggtca
    10021 ccagcccctc ccatccggaa cccggctcga tgaccgtctc caccggttcc ctgaggagca
    10081 ccggttcccc cccggcgata cggatgcggg accggacggt caccgcccag atcctccccg
    10141 agtcaggatt atagggaaag gtcagtgcta ccatggagca ccctggtctt tgataagggg
    10201 gtatagttca cagggtgcca cgagagggat tattccggtt cttgtcacgt acagcactat
    10261 tctcccgctg gaggtaaaat cggtttcgaa aatgacctgt ccgactggaa caacctatgc
    10321 gccgggtgag agcacaaatg aagactactt tcggtaagga aggatgcaat ctgcccggga
    10381 aaacaggaac gacatatttt tccatgaaca taccgaccca gttgcatgag ctgtcgtacc
    10441 tggctgtttc ttctctgtgt cctgctggtt atcacacctg caaatgccct ctgtcactgg
    10501 gcccccgaag taacccctcc ccccggggtc ccgccttgca gcgggagcta cgactcccgc
    10561 cagcagccct ggccccgcct tccgaccgat accgagatag agagggcaaa ggaggcgtat
    10621 ctcgcctatc agctggaagg gagcgagagg cgccggatcg attcggtagt ggtggcgaag
    10681 taccatacct gcgaggggac ctggatcaac catgtcgata tggaggtgaa gtactcctgg
    10741 tggtgggagt cggtcaccgg agagggtgaa tggcaacctt cagctgccta taccttcctc
    10801 atctatgatc ccgggagcaa tcggttcgag tttgtgctgg acatcggggt gagtccctat
    10861 ttcatgggtc ttcgaactga cgcaatccag gggtttgcaa ccggcccctg ctccagtggg
    10921 ggaggagcag tacccacgtc ttccggcggg agtgcagccc ctgaatctga tgacaactcc
    10981 tgggttgtga tcatcggtgc catcgcggtc atcggaggag gactggcgat cggcgcaaag
    11041 aaacttgccg ggggaaaagg aaaacaaccc tccggggaga aaaagaagga gaagaaggag
    11101 caggagaagg tccgctacat cctccagctc agcgccgacc gggtgacggt caccccggcg
    11161 gcgcccgcac accttacggt gaccgcgtgg aagattgtgg ggaccagccc cccggccccg
    11221 gcacccgaag catccatcac gctgaccgtc ccggcaggga acgaagggct ctccgtccag
    11281 ccggcctccg gaaccggctc ggtggaggcc ggtatcgctc tcgtatccca ggtcgcaaag
    11341 agccctgtgc tgatcacggt aacggcatcg gcggggaaga gctcggtatc ggcacaggtg
    11401 accgtcgaga tcccggcaga gtacctgatg gagttcttct gatccggaaa agacatcccc
    11461 ctttttttat accaacgcag ggaaacatac tctccataga ggtgatccat gccctactgt
    11521 gaaacctgtg gcaccgaact gaatgagggt acatcctgcc cgtcatgcgt gagcggcgag
    11581 ggacgaacga acgccccttc gctggaagga ctgatatgga cagcaaaagc cccggtcatc
    11641 tccagccggc cggtagtcac ccagctgatc ctggtctttg tcatcgcctg tctgattgtc
    11701 ctcgcattcc tgtttctcct ggacatcgac gccggactgg gtgcagcccc gtatatcctg
    11761 gggatcatgg tcttttttat tgtactcggt ctgatcatcg ccgctgcgat ccagttcttc
    11821 accaaggggg gcccaaccac cgaatttgcg gtgaccccgg agggcatcgg gtaccgggca
    11881 ggaaaagagt caaagaccat caaccgggca acgcttgcag ggaccgccct tggcggctca
    11941 ctgtcaggag caggggggag catgataaac atcagccgtg agatggactt catggcatgg
    12001 gacgaggtcc ggagcatcac cgtatacccg agagaacggt cactcgtctt ctaccggagc
    12061 atgctcatct tcccctttgc tctctactgc actccggaga acttcgggca ggtcaaggca
    12121 cttgccaaaa agtatgcacc tgatgcccgg tataaggaaa agggctggtg agacgacatt
    12181 cagaatatca gagtcattcg ccagggcgcc atggcgctcg ccatcggcat cctcgtattc
    12241 atggccggtg agtttgtggg gtcatattcg gggatagccg agacagccgg tgtactgatt
    12301 gcagtcg
//
LOCUS       DAOOCO010000039        12304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_4796_length_12304_cov_5.297657, whole genome shotgun sequence.
ACCESSION   DAOOCO010000039 DAOOCO010000000
VERSION     DAOOCO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_12304_cov_5.297657"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3658
                     /locus_tag="PK154_03605"
     CDS             <1..3658
                     /locus_tag="PK154_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10182.1"
                     /translation="LSFECGKTFILNISPGTLDVTMDKKGSFTARVLEEREGQYYPAT
                     DASISVETGPEVAKILKVSPPWGQNELAGTVIQSRFSTVKSSDLVVTARMDDGGIQSG
                     TIKVNLARESIGELVVVFDPESKTSVSPFIRGDSVTLKAKVVPPFGEAPVQAEIEFAC
                     ANPRGWLVGPKNTTSVAGSIAGSTADIPPVPVPGEEGWESAVFMGSNPVPAVQGIPPA
                     MEPVIVTARKDGVVIDRAVVQVGLLGRPMIVVDKERFNFLANADIQRDGRPPAVTTGN
                     ATITVENHGGYPWKIRGEPAGDSKYIEVKEAFRSPTSTSFEFTLKNPVPRPENEGGKP
                     GWEQQVLVHTYANLVTAGKLGAVEIQPDLEVQGPDILIRILHEGLFVEATYEYDEQGV
                     RHEIVGKKDLVIRVDIPSEEKYIPPEIKVSALIWDGSNLVPDPTVYIDHVKEPQESKD
                     RNPKARAYWNFIFLTMKGISIEFFRIPEGIHTYHSAPLTTSIWKVWISKKIPSNGEIL
                     RGHILFFAKKQFDGDLHPDETYLPQFGSMVSGTPGVSFERDTTWQLEVPVELLLGTKG
                     AIGSEDNIGSVIEEGARCHKIIIESFPPERQKDLVAQIDGLPGKSARDYRKFSDSLFD
                     QAHEYWASEQKSYLLWEGVWSTSIWVLEKTVVAGDIAFQVLVGYATKNLGPWTAYFTS
                     TLATTLKNESVNFYLFYAVEQREMEVCARLYVEREWKTFLEGVVTGAVDATILKDFRI
                     EKVVTDPKRCLRTVAWLWLWKFGRNLYHDPDAGYYVAMKKATEELGEVALVVAIQHYV
                     NENGTKDIRQLFKEARQKGYFGGTATEPEPKTAKPLKSSARSADQNARDAFIRGRDAG
                     RAKVEDLSRAAGDLTRNPGDPEAIRRFGKAAEAVQGDKHAMHALNEQNPNRQDPLRQQ
                     FNDFWKGEYAAVDRATRQRIADQLNTRLKPGDTPYTADDIGVAGVTNTPPKSSAEDSV
                     KSTYDRDVTYRDNRTGKDITTGVSRDIYNEEFYRQRHNGDLPMKNEGGRPVVDRDAVN
                     TFAEHCDQTVTDRYHPDAYGGGPEDIGPAVDPKQKGVPFKDVEATAKAMEYKVEEWYN
                     RAQKAEGSGDYAAAEGYKEEGMRQLTKQFKNQVQGRVDKINEIAGTPDAPVARVPDDL
                     AAAVDVMNRVGTTGPDGTIFTPADAEAALSSMNTSPKDVASKMSGLVESLQKNAPPDV
                     RKVVEDHVKTLTDI"
     gene            3676..4530
                     /locus_tag="PK154_03610"
     CDS             3676..4530
                     /locus_tag="PK154_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW10183.1"
                     /translation="MTTKFCPKCGIPLSPGAKFCPGCGQKISDSAPAGKGSLQRAAEA
                     IQTVSSTISKVESATQSAKTVVETARDLSTITIRPPAQWKVVVGEVLPVAGKKVVDAA
                     VSTAGQKVEQCVTEKASRVITEHRDGAAQTPVPESFVPGKGIPGEAGGVPCPSCGKPM
                     IPREKFCESCGAQVDKGTKPAPAPSQVCRSCGKPVIPGKKFCGSCGAKIEEITKPVPA
                     PPLQPVCPACGNAIIPGKKFCGSCGAKIEEMTKPAPVPPPQPGCPACGTPVIPGKRFC
                     GSCGQKLP"
     gene            4627..5193
                     /locus_tag="PK154_03615"
     CDS             4627..5193
                     /locus_tag="PK154_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10184.1"
                     /translation="MPFMQGFFTYLPLALLSALMVLAGTGSGANFSDDIAAEAEVKAF
                     SSVDNASLAVYAGQNALQSGRYDEAVEHFTEAVEADPTWMAAWYLKAYSLSRLNRTDE
                     ALAAVDQALLLDLKDRDSNNLKADLLESLGRGAEAARYRSLADTLSVTVQPTTPVTAE
                     TTTKAPISPFVGLSSILVTAYLIRIRER"
     gene            5228..5968
                     /locus_tag="PK154_03620"
     CDS             5228..5968
                     /locus_tag="PK154_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10185.1"
                     /translation="MKNHEWKWVYLSALGVICFLFLIGYANEAMDCMSGGCAVMFISF
                     FLVITLIAVALLFWSRARTVDAILAGQDLLAHWIYSPDDSALSADREFAEYKEANKAL
                     FIIVGGFFLFAIIIVLIFGGEAGGLTALILTCALGIIALAAWGAPRIVYRRSLQAIPE
                     AYIGRNGVIYREAVYPFRSFLNRLEGVTYREAGFGNPPLLTFSFLQVVGLYILRPYAV
                     SVPVPAGEEDHAREISGMFVGGGGTRYP"
     gene            complement(6088..6456)
                     /locus_tag="PK154_03625"
     CDS             complement(6088..6456)
                     /locus_tag="PK154_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10186.1"
                     /translation="MAERPLGITILAILWILGGLFWIMAGLLGGAVLGSIGLGILGAA
                     LGIILFIIGIIDLLIGIGCLKGWGWIWTVGVIVVIINIALHLLALVSGGIGAILGLAI
                     GILILWYLFQPNVKAFFGKE"
     gene            7056..7286
                     /locus_tag="PK154_03630"
     CDS             7056..7286
                     /locus_tag="PK154_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10187.1"
                     /translation="MDLADLKRKRDCIEHGDLRDSQGLFQKAIAAYDHALAIDPEDAD
                     AWYNKGETLFKIGKTEEAVGCFETATRLYLGE"
     gene            complement(7325..7672)
                     /locus_tag="PK154_03635"
     CDS             complement(7325..7672)
                     /locus_tag="PK154_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10188.1"
                     /translation="MTDTALTALLYNLERWRLWGTIIITILGLFIAVGVIFILLRVDE
                     IDAQGISTVVGLFTTVLGTIVGAFLGLQFGLGEKAKDNTIRLNNTRVALEAIAKVSST
                     DRNTADELHKRLL"
     gene            7831..10335
                     /locus_tag="PK154_03640"
     CDS             7831..10335
                     /locus_tag="PK154_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPW10189.1"
                     /translation="MTVFTVATLPEEEILEQLGTSLPDGLSSGEADARLRQSGFNEIT
                     ADKVEWWEIALRQFRSAFIYLLLVAALIVLVIGEYIDAAVIIGFVIINAALGFYQEYR
                     SEQSLRALQQYIRPKSRVKRDAGWHTIDSRILVPGDIIQIVPGDMVPADVRVLRSQNL
                     VVNETVLTGESIAVPKRPELLPVEPQTIYESHNLCFSGTSVVGGEAVAVVVETGVRTA
                     MGTIAKLTVETTKESEFSKGIGRFSRFILRMILVTLVFVFVANLLLKGDSVDIIELVV
                     FSIALVVSVVPEALPVVTTVSLSKGALQLAQQNVVVKRLTAIEDIGSIEILCSDKTGT
                     LTKNTLSVTGLSPGADPRLLSYALLAVAETKEKTEPFDIAIVEGAERFPRVEIGISER
                     LGELPFDPHTRKNAVLVRSASEIVMVVRGAPEEILRVSPSFQGQEREALTSWLSEKGR
                     AGERTIAIAKRGMPPGTTGFTPADIQDLEFLGAIAFTDPIKPSTFHAIEMARELGVAI
                     KIITGDSAEVAGAVGMEIGLIRDPGMVITGAALMALPPYEREQAMHEHAVFARVTPEQ
                     KYEIIQTLQRTRTVGFLGEGINDAPALKLAGVSLVVESAADIAREAADIILLQKNLEV
                     IIEGIRSGRAVFANSVKYLKATLSSNFGNFYAIAIVSLFIPFLPMLPLQILLVNLLSD
                     FPMISIATDTVDEAELRRPGHYDLKEIVLIATLLGVVSTVDDFAFFAIFVGEGAGVLQ
                     TSWFIGSILTELVFLFCIRTRLPFYKASRPSGYVIGLTVAAAALTILIPYTSFGQTLF
                     SFIPPSLPALGIILGLVVAFFICAELVKLAYYRYMD"
     gene            complement(10427..11317)
                     /locus_tag="PK154_03645"
     CDS             complement(10427..11317)
                     /locus_tag="PK154_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW10190.1"
                     /translation="MAFEGATVRGKVVKSMRQIAIYGKGGIGKSTIASNLSAALSERG
                     HTVMQVGCDPKRDSTRILAGGRFIPAVLEEHREQLRVGRDEYAIKLGNIIYRSPGGIY
                     LVEAGGPEPGVGCAGRGVLTALQILKDLKAFETYGIDVAIYDVLGDVVCGGFSMPIRE
                     GFAKEIYLICSGGFMSIYAANNIARAVQRLAKRGDVGLAGIICNSNGNEALEHAVIPA
                     FAEKLGTSFVRFVPRSPVIQACELEGRAVVEHAPDSKEAEIFRDLAKAIMENASRVIP
                     TPVAELAELEAMYREHIPKQ"
     gene            complement(11278..>12304)
                     /locus_tag="PK154_03650"
     CDS             complement(11278..>12304)
                     /locus_tag="PK154_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW10191.1"
                     /translation="GPEIVGDDIRAVCETLQPEVKFRLIPIQSPGFRGSQYDGIDSAL
                     DTILKSLVKENNEKIPHSVCLIAPHANANPTWTADLAWVKQVLSRMGIPVVATLTHRT
                     PLSEIEHAASAETSLLLSHDAGQNAVEYLKEAHCIEPIGRGIPLPIGMTNTQRWLAAL
                     GERFDRQKVVEEMIAEGERMVMATCRRKWPMARFLYRTPAAIVSDATVGIPLVRFITE
                     EMEMTPELVALYTSHRPVRDLLESELKDLKLTPKVVYGADVYRTRMSLLETKPRVIFG
                     STIERHASEGLSPVYTVEVVRMIRQFRMINREYFGYTGILNLFECVQNEWIMGWRSKE
                     RRYGAKW"
BASE COUNT         2731 a         3415 c         3538 g         2620 t
ORIGIN      
        1 gctgtcgttc gaatgcggga aaaccttcat cctcaacatc agtcccggca cccttgatgt
       61 cacgatggac aagaagggat ccttcactgc cagggtgctt gaagaacggg aggggcagta
      121 ttacccggca accgacgcat cgatctcggt tgaaaccgga cctgaagtcg cgaagatcct
      181 gaaggtctct cccccctggg gacagaacga gctggcaggt accgtcatcc agtcccgttt
      241 ttccacggtg aagtcttctg atctggtagt taccgccagg atggacgatg gcgggatcca
      301 gtcagggacg ataaaggtca acctggcgag agagagcatc ggggaactgg tcgtggtctt
      361 tgacccggag tccaagactt cggtcagccc gttcatccgg ggagattcgg tcaccctgaa
      421 ggcaaaggtc gtcccaccct tcggagaagc tcctgtgcag gcagagattg agtttgcctg
      481 cgcaaatcca cggggatggc tggtcggccc gaagaatact acctccgttg cgggctcgat
      541 agccgggtca actgccgata ttcctcccgt ccctgtgccc ggcgaggagg gatgggagtc
      601 ggcggtcttc atggggagca acccggtccc cgcggtccag ggcatcccac ctgcaatgga
      661 accggtgatc gtcacggccc ggaaagacgg ggtggtgatc gaccgggcag tggtccaggt
      721 cggcctgctc gggcggccga tgattgtggt tgacaaggaa cggttcaatt tcctggccaa
      781 tgccgatatc caaagggatg gccgcccgcc tgcggtcacg actggtaatg ctacgatcac
      841 ggtggaaaac catgggggat atccatggaa gatacgggga gagcccgcgg gtgacagcaa
      901 gtatatcgag gtgaaggagg ccttccgatc tcccacctcg acatcgttcg agttcacttt
      961 gaaaaatccg gtaccacgcc cggaaaacga gggaggtaag ccggggtggg agcagcaggt
     1021 cctggtccac acctatgcca atctcgtcac tgccgggaag ctcggagctg tggagatcca
     1081 accggatctg gaggtccagg ggcctgatat cctgatccga atcctgcacg aagggctctt
     1141 tgtggaagcg acctatgagt acgatgagca gggggtccgg cacgagatcg tcggaaagaa
     1201 ggacctggtc atccgtgtcg atatcccttc cgaagagaag tatattcctc cggaaatcaa
     1261 ggtcagtgca ttgatctggg acggttctaa tcttgtaccg gatccaactg tatacatcga
     1321 tcatgtgaaa gagccccagg aatcaaaaga caggaatcca aaagccaggg catactggaa
     1381 cttcatattc ctgacaatga aagggatatc gatcgaattt ttccggattc ctgaaggtat
     1441 ccacacctat cattcggctc ccctcaccac atcgatatgg aaggtatgga tcagcaaaaa
     1501 aataccgtca aatggggaga tactgcgggg tcatatcctc ttctttgcca agaaacagtt
     1561 cgacggggac ctgcatcccg atgagacgta ccttccacaa ttcggatcca tggtgagcgg
     1621 gacccctggc gtgagcttcg aacgcgatac cacctggcag ctggaagttc ccgtagagct
     1681 gttactgggg acgaaagggg ccattggatc ggaggacaac atcggcagcg ttatcgagga
     1741 gggagcacgt tgccacaaaa tcatcatcga gagttttccc cccgaacgcc agaaagacct
     1801 ggttgcccaa atcgatggac tccccgggaa aagtgcaaga gattaccgca agttcagcga
     1861 ctcccttttt gatcaggcac atgaatactg ggcttcagaa cagaaaagct accttctatg
     1921 ggaaggcgtc tggtcgacat cgatatgggt gttggaaaaa accgtcgtcg caggtgatat
     1981 tgcgttccag gtgcttgtcg ggtatgccac aaagaatctc gggccgtgga ccgcttattt
     2041 tacctctacc cttgcgacga ccctgaagaa tgaaagcgtc aacttctatc tcttctacgc
     2101 tgtggaacaa cgcgagatgg aggtctgtgc ccgcctgtat gttgagcggg aatggaaaac
     2161 gttcctcgag ggagtggtta ccggagcggt tgatgccacg atcctgaagg atttcaggat
     2221 cgagaaggtc gttaccgatc cgaaacgatg cctgagaacg gtggcatggt tatggctatg
     2281 gaaatttgga cggaatcttt accacgatcc tgatgccggt tactatgtgg cgatgaagaa
     2341 ggcgacggag gaactggggg aggtggccct tgtcgttgca atccagcatt acgtcaatga
     2401 gaacggcaca aaggacatca ggcagctgtt caaagaggcc cgtcaaaagg ggtatttcgg
     2461 aggtactgct acagaacctg agccaaagac cgcaaagccc ctgaaatcat ctgcccgtag
     2521 cgccgaccag aacgcaagag acgcctttat tcgcggcagg gacgccggca gagcgaaagt
     2581 cgaggatctc agcagggcag ccggtgatct caccagaaat cctggggatc ccgaggcgat
     2641 ccgcaggttt gggaaggcag ccgaagcggt gcagggggac aaacatgcca tgcacgcgct
     2701 caacgagcaa aatcccaacc ggcaggaccc gctgcgacag cagttcaacg atttctggaa
     2761 gggagagtat gcagcggtcg acagagcgac acggcagcgg atcgcagatc agctcaatac
     2821 ccggctcaaa cccggggata ctccgtatac agctgatgat atcggagttg ccggagtgac
     2881 aaatacgccg ccaaaaagct cggctgagga cagcgtcaag tcgacctatg accgggatgt
     2941 tacctaccgg gacaaccgca cgggaaagga tatcacaacc ggggtctcgc gggatatcta
     3001 taacgaggaa ttttaccggc agcggcacaa cggggatctg cccatgaaga atgagggcgg
     3061 gcggccggtg gttgacaggg atgcggtcaa cacgttcgca gagcactgtg accagacggt
     3121 aaccgaccgg tatcaccccg atgcctatgg tggtgggccg gaggatatcg ggcctgcagt
     3181 cgacccgaag caaaaggggg tgccgttcaa ggatgtcgaa gccaccgcga aggcaatgga
     3241 atacaaggtc gaagaatggt acaaccgtgc ccagaaggcc gagggttccg gagactatgc
     3301 agccgcagag gggtataagg aagaggggat gcgacagctg acaaagcagt tcaagaacca
     3361 ggtccagggg cgggtcgata agatcaacga gattgcaggg acaccggacg ccccggttgc
     3421 ccgtgtgccg gacgatctgg cggctgcagt ggatgttatg aaccgcgtgg gaacgacggg
     3481 acctgatggt accattttca ctccggcaga tgccgaggca gccctgtcat caatgaacac
     3541 ctccccaaag gatgtggctt ccaagatgtc cgggttagtc gaatcgctcc agaagaatgc
     3601 gcccccggac gttcggaagg ttgttgaaga tcacgtgaaa actcttaccg atatatgaga
     3661 agggagtaga gaacgatgac aacaaaattc tgcccgaaat gcgggatacc cctctctccc
     3721 ggcgcgaagt tctgcccggg ctgcggccaa aagatctctg actcagcacc ggcagggaaa
     3781 ggatcgctgc agagggcagc agaggcgata caaacggtct cttcgaccat ttcgaaagtc
     3841 gaatccgcaa cacaatcggc caagacggtt gtagagactg cccgggatct ctccacgatt
     3901 acaatccgtc cccctgctca atggaaggtg gtggttgggg aagtcctccc ggtggccggc
     3961 aagaaggtgg tcgatgcagc cgtatcaact gcagggcaga aggtcgagca gtgcgtgact
     4021 gaaaaggcat cccgggtgat cacagagcac agggatggag cagcccagac ccctgtcccg
     4081 gaatcattcg tgcctggaaa agggattccc ggggaggcag gcggggtgcc gtgcccctcg
     4141 tgtggaaaac caatgattcc ccgggagaag ttctgcgaga gttgtggggc acaggttgat
     4201 aaggggacaa agcctgcccc tgccccatcc caggtctgcc ggtcctgcgg aaaaccagtg
     4261 attcccggga aaaagttctg cgggagctgt ggtgcaaaga tcgaagagat tacaaaacca
     4321 gtaccggcac ctccccttca gccagtctgc cctgcatgtg gaaacgccat cattcccggg
     4381 aaaaagttct gcgggagctg cggtgcaaag atcgaagaga tgacgaaacc agcaccggta
     4441 cctccccctc agccaggctg tcctgcttgt ggaacccctg tcatcccggg aaagaggttc
     4501 tgcgggtcgt gcgggcaaaa actgccctga attttttata acctcactga tccggagact
     4561 cccggcccta atttaccgtc ccggattcag cacctcgcaa ataaccttga ttacctctcc
     4621 cgccgaatgc catttatgca gggatttttc acgtatcttc ccctagcgct tctttctgct
     4681 ctcatggtcc ttgccgggac tggctccggg gccaattttt ctgacgacat cgctgccgag
     4741 gcggaagtga aggccttttc atcggtcgat aatgcctcac ttgccgtata cgccggccag
     4801 aatgccttgc agagcgggcg gtatgatgaa gccgttgaac atttcaccga agcggtggag
     4861 gccgatccaa cttggatggc agcatggtac ctgaaggcat acagcctttc ccggctgaac
     4921 cgcacagacg aggcattagc cgcagtcgac caggcactcc tccttgatct aaaggaccgt
     4981 gactcgaaca acctcaaggc agaccttctc gaatcgcttg gcaggggagc agaggcagcc
     5041 cggtaccgaa gcctcgcgga caccctttca gtgacagtgc agcccaccac cccggtaacg
     5101 gctgaaacca ccacaaaagc cccgatcagc ccgtttgttg gcctttcttc gatcctggta
     5161 acggcctatc ttatccggat acgggaacga tgacgatcac caaggatttt aaaaatacag
     5221 ggcaattatg aagaaccacg agtggaaatg ggtgtatctt tcagctctcg gggtgatatg
     5281 cttcctcttc ctgatcggct atgcaaacga ggcgatggac tgcatgtccg ggggatgtgc
     5341 ggtcatgttc atctcgttct ttctcgtcat caccttgatc gcggttgccc tcttattctg
     5401 gagccgggca aggacggtgg acgcgatcct tgccggacag gacctcctcg ctcactggat
     5461 ctattcccct gatgactcgg cattgagtgc cgatcgcgag tttgccgagt ataaggaggc
     5521 gaacaaggct ctcttcatca ttgttggggg ctttttcctt tttgcgatca tcatcgtcct
     5581 gatctttggc ggggaggcag ggggtctcac tgcattgatc cttacctgtg cccttgggat
     5641 tatcgcactt gctgcctggg gcgccccgag gatagtatac cgcaggtctt tgcaggcgat
     5701 ccctgaagca tatattggaa ggaacggggt tatctaccgg gaggccgtgt acccgttccg
     5761 ttccttcctg aaccgtctcg agggcgtgac ctaccgggaa gccggcttcg ggaatccccc
     5821 cctgctcacg ttctcgtttc tccaggtagt gggcctctat atcctccgtc cctacgcggt
     5881 cagcgttccg gtcccagcgg gggaggagga tcatgcccgg gagatttccg ggatgtttgt
     5941 agggggtggg ggtacacgat acccgtgaat cgatcgaatc ctttctgttt tatcttccgt
     6001 atcgggaaat ggaattttga atgtccctcc ccttctcgat cgatgaagga accgacaaca
     6061 cgggcatgaa gaaaaataat ggtgcttcta ctccttcccg aaaaaggcct tgacattggg
     6121 ctggaacagg taccacagga tgaggatgcc gatggcaagc ccgaggatcg caccgatgcc
     6181 tccggagacc agggcaagga ggtggagggc aatattgatg atgacaacga tgaccccgac
     6241 cgtccatatc cagccccagc ccttgagaca cccgatgcct atcaggagat cgatgatccc
     6301 gatgatgaag aggatgatcc ccagagcggc accaaggatc ccgagcccga tggatcccag
     6361 caccgctccg ccaagcaacc cggccatgat ccagaaaagc ccgccgagga tccagaggat
     6421 cgcaagaatg gtgatgccca atggtcgttc tgccatgatg gtggtttgga ctgattatgt
     6481 catataggtt ctttttccag tgtcagtcat cgaaaaaatg aacaatctcc aaaatcctct
     6541 ctctccgcac ctcccttctg ggatcggata ccactgttcc actcaaacta tgactgcagc
     6601 taccactgct cccactccgg gctgttctgt accctctgac tgcccctccc ctcccccacg
     6661 ttcctgaatg gtgttctgtt atgattgatt caacagatac aagtcagcca gattcggatc
     6721 ggacatcctg gattctgaca tttctctatc cactggaagt gaaggttaat cttctcttgt
     6781 taacagagcg agaacgggga gaattgtcgc aggaggatgc gacacctgcc gaaatatcat
     6841 acccctttat ttccagtgaa tggaagctgg tgtcaccata ataaacactc ggttttccca
     6901 ttgctgtgta cgccggcaat ctgatccccc atccctccca tccaatctct gcaatgatat
     6961 ggtgacaacg ttgccgtgcg ctttcattgg gcaaattaat ataattcaat gaatattatc
     7021 aagataacaa cgaaaaatgg ggtgtcccgg cagcaatgga cctggccgat ctcaagcgga
     7081 aacgggattg catagagcat ggcgatcttc gcgacagcca gggccttttt caaaaagcca
     7141 ttgcggccta tgatcatgcc cttgccattg atcccgaaga tgctgatgcc tggtacaaca
     7201 agggggagac cctgttcaaa ataggcaaga ccgaggaggc tgttgggtgt ttcgaaacag
     7261 caaccaggct ctacctcggt gaataagctc gttgttcaaa tcatcagtct caaaaatgag
     7321 tgagttagag gagccgcttg tgtaattcat cagcggtatt acggtcagtt gatgatactt
     7381 ttgcgatggc ttcaagggca accctggtgt tgtttaaccg gatggtatta tctttagctt
     7441 tctctccaag cccgaactga aggcctaaga aagccccgac tatagttcca aggaccgtag
     7501 tgaagagacc gactacggtc gaaattccct gggcatctat ttcatcaacc ctcaatagga
     7561 tgaatataac cccaactgca atgaaaaggc caaggatagt gataataatg gtaccccaaa
     7621 gtctccatcg ttcaaggttg tagaggagag cggtaagagc ggtgtctgtc ataaatggga
     7681 ataattaact tctttcattt ataattttct ataaataata tcgagattat ccaagcattt
     7741 acctctattc tctgcctcta tcaaacatgg gttcaaatct cttaagacga atcatcatga
     7801 tagtatggtg agaggcgaac cgcactcctg atgaccgttt ttaccgttgc caccctgccg
     7861 gaagaggaga tcctggagca gctggggacg tcgcttccgg atggactctc gtccggggag
     7921 gcagacgcac gattacggca atcgggattc aatgagatca ccgccgataa ggtcgaatgg
     7981 tgggagatcg cgctccggca gttcaggtcg gcgttcatct acctgctcct cgtggcagcg
     8041 ctcatcgtcc ttgtcattgg cgaatatatc gatgcggcgg tgatcatcgg gtttgtgatc
     8101 atcaacgcag cgctcgggtt ttaccaggaa taccggtcgg agcagtcgct ccgggcactg
     8161 cagcagtata tcaggccgaa gagccgggtg aaacgggatg ccgggtggca caccatcgac
     8221 tcccgcatcc tcgtgcccgg ggatatcatc caaatcgtgc ccggggacat ggtcccggcc
     8281 gatgtgagag tcctgcggtc gcagaacctt gtcgtcaacg agacggtgct caccggtgag
     8341 tcgatcgcgg tcccgaaacg gccggaactc ctccctgtcg aaccgcagac gatatatgag
     8401 agccacaacc tctgtttctc cggcacttct gtcgtcgggg gggaggccgt tgccgtggtg
     8461 gtggagaccg gggtccgcac ggctatgggg acgatcgcaa aactgacggt agagaccacg
     8521 aaggagagcg agttctcgaa agggatcggc aggttcagcc ggttcatcct gcggatgatc
     8581 cttgttaccc tggtgttcgt gttcgtggca aacctgctcc tgaagggaga ctcagtggat
     8641 atcatcgaac tggtggtctt ctccatcgcg ctcgtggtca gcgtggtccc ggaagctctt
     8701 cctgtcgtga cgacggtgtc gctctcgaag ggggccctgc agctggcaca gcagaacgtg
     8761 gtggtgaaac ggctcacggc gatcgaggat atcgggagca ttgagatact ctgttcggac
     8821 aagaccggga ccctgaccaa gaataccctg tcggtgaccg ggctctctcc cggggcagat
     8881 ccccgtctcc tctcctatgc tctcctggcc gtggcagaga ccaaggagaa gaccgagcca
     8941 tttgatattg cgatcgttga gggtgccgaa cggttcccac gggtggagat cgggatctca
     9001 gagcgtcttg gcgaactgcc cttcgatccc cacacgcgga agaacgccgt gctggtccgc
     9061 agtgcgagcg agatcgtgat ggtcgtccgt ggtgcccccg aggagatcct gcgggtgtct
     9121 ccctcgttcc aggggcagga gcgggaagcc ctcacctcct ggctctcaga gaagggacgg
     9181 gcgggcgaac ggacgatcgc gattgcgaag cgggggatgc cgcccgggac cactgggttt
     9241 accccggctg atatccagga cctcgagttt ctcggtgcca tcgcgttcac cgacccgatc
     9301 aagccctcca cgttccatgc catcgagatg gcacgggagc ttggggtagc gatcaagatc
     9361 atcacggggg acagtgccga ggttgcgggt gccgttggga tggagatcgg cctgatcagg
     9421 gatccaggta tggtgatcac cggagcagcc ctgatggcat tgcctccgta cgagagggag
     9481 caggcgatgc acgagcatgc ggtctttgcc cgggtcaccc ctgagcagaa gtacgagatc
     9541 atccagaccc tccagcggac ccggacggtc gggttcctgg gagaggggat caatgacgcc
     9601 ccggccctca agcttgccgg ggtgtcgctc gtggtggaga gtgcggccga tatcgcccgg
     9661 gaagctgccg atatcatcct gctccagaag aatctcgagg tgatcatcga ggggatccgg
     9721 agcgggaggg cggtctttgc caacagcgtc aaatacctga aagccaccct ctcctccaac
     9781 ttcgggaact tctatgcgat tgccatcgtc tcgctcttca tccccttcct cccgatgctg
     9841 ccgttgcaga tcctgcttgt caacctcctc tccgatttcc ctatgatatc gatcgccacc
     9901 gataccgtgg acgaggcaga actcaggcgg cccgggcatt atgacctgaa ggagatcgtg
     9961 ctgatcgcaa ccctcctcgg ggtggtgagc accgtcgatg acttcgcgtt ctttgccatt
    10021 tttgtaggag aaggggccgg ggtgctccag accagctggt tcatcgggag catcctgacc
    10081 gagcttgtct tcctcttctg tatcaggacc cggctcccgt tctacaaggc gagccgtccg
    10141 tctgggtatg tcatcgggct gacggtggct gctgccgccc tgaccatcct catcccctac
    10201 acctcgtttg ggcagacgct cttctccttt atcccgccct cgcttccggc gctcgggatc
    10261 atcctcggcc tggtggtcgc attcttcatc tgcgccgaac tggtgaagct tgcctattac
    10321 cggtacatgg actgatattc acgttgctag gatcaggtgc ggttctggag agggagtgca
    10381 tcagaaaaga aggttagttg agttcatccg gaccgggcgt atctccttat tgtttcggga
    10441 tgtgttcccg gtacatcgct tcgagctccg cgagttcagc caccggggtt ggaatcaccc
    10501 gggaagcgtt ctccatgatc gctttcgcaa gatccctgaa gatctcggcc tccttcgagt
    10561 ctggggcatg ctcgaccacg gctctgcctt ccagctcgca ggcctggatc acgggactcc
    10621 tcgggacgaa ccggacaaac gatgtcccga gcttctctgc aaaggccggg atgacggcat
    10681 gctcgagcgc ctcgttgcca ttgctgttgc agatgatccc ggcaagcccg acatctcccc
    10741 gctttgccag gcgctgcact gcccgggcga tgttgttggc tgcatagatg ctcatgaatc
    10801 ccccggaaca gatgaggtag atctcctttg caaacccttc tcgtatgggc atcgagaatc
    10861 ctccgcagac cacgtcgccg aggacatcgt agatcgccac atcgatccca taggtctcga
    10921 aggccttgag atctttcaag atctgcaggg cggtcagcac cccccggccg gcacacccga
    10981 cacccggctc cgggcccccg gcttcaacga ggtagatccc gcctggtgat ctgtagatga
    11041 tattccccag cttgatggca tattcatccc tgccaaccct cagctgttcc cggtgctctt
    11101 caaggaccgc cgggatgaag cgccccccgg ccaggattct tgtcgagtcc ctcttgggat
    11161 cacagccgac ctgcatcacc gtatgccccc tctcgctcag tgctgcagac aggttcgagg
    11221 cgatcgtgct cttcccgatg cctcctttcc catatatcgc gatctgtctc atgctcttca
    11281 ccactttgcc ccgtaccgtc gctccttcga acgccatccc atgatccatt cattctggac
    11341 gcattcaaac aggttcagga ttccggtata cccaaagtat tcccggttga tcatccggaa
    11401 ctggcggatc attctcacca cctcaaccgt gtagaccggg gagagtcctt cgcttgcatg
    11461 ccgctcgatg gtgctcccga agatgacccg gggctttgtt tccaggaggc tcatcctcgt
    11521 ccggtataca tccgccccgt acaccacctt cggtgtcagc ttcaggtctt tcagttcact
    11581 ttcaagcagg tcccgcacgg gacggtgtga agtgtacagg gcaaccagtt ccggggtcat
    11641 ctccatctcc tcggtgataa acctgaccag cgggattccc accgtggcat cagagacgat
    11701 tgcggcaggg gtccggtaca ggaaccgggc catcggccat ttgcgccggc aggttgccat
    11761 caccatccgt tcaccctccg caatcatctc ttcgacgacc ttctggcggt caaaccgttc
    11821 tccaagagcc gccagccagc gctgggtgtt ggtcattccg ataggaagcg ggatccccct
    11881 gccaatcggc tcgatgcaat gggcttcttt gagatactcc acggcattct gcccggcatc
    11941 atgggagagc aggagtgatg tctcggcaga tgcggcatgc tcgatctcgg aaaggggtgt
    12001 ccggtgggtg agcgtggcaa caacagggat tcccatcctc gagagaacct gttttaccca
    12061 tgcaaggtct gccgtccagg tcggattggc gtttgcgtgc ggggcgatga ggcagacgga
    12121 gtgtgggatc ttttcattgt tctccttcac caggctcttc aggatcgtat ccagggcact
    12181 atcgatgccg tcgtactgtg atccccggaa gccaggggac tggatcggaa tgagccggaa
    12241 cttcacctct ggctggaggg tctcgcagac ggcacggatg tcgtcaccga caatctcggg
    12301 gccg
//
LOCUS       DAOOCO010000040        11724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5126_length_11724_cov_5.594824, whole genome shotgun sequence.
ACCESSION   DAOOCO010000040 DAOOCO010000000
VERSION     DAOOCO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_11724_cov_5.594824"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK154_03655"
     CDS             <1..421
                     /locus_tag="PK154_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10192.1"
                     /translation="LLIDLPSFSPGLIGTPSFLQILGIYHNDELGRLNYDSRIILINN
                     GSDSLGNQNLTAEFYRNGIKIPAVVETMNGDLFISTRHYGIQTMGGLGCSGPTWLPKE
                     KIALDFSDGTFHPGDTIRMDVFSKTSATLISRHTFIA"
     gene            complement(554..814)
                     /locus_tag="PK154_03660"
     CDS             complement(554..814)
                     /locus_tag="PK154_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10193.1"
                     /translation="MKNPRSQPSALRIIAGVILGIVVGFFVFFGIALILGALNIPVSM
                     QFAENIFSLIVFVLSMIICIALVLWLVWTTPPTEPDQEAGSE"
     gene            complement(917..1483)
                     /locus_tag="PK154_03665"
     CDS             complement(917..1483)
                     /locus_tag="PK154_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HPW10194.1"
                     /translation="MHFAKKGLRVLGIAESFSSRKNSILAGVVMRKDLRLDGFSYTCT
                     TVGGMDATDSVIDLYRNFSREDINFIMVGGAVISWYNIIDPLRIFQETGRGTIIATYK
                     HSEGLIENIIHHFPGDEGRISAYRSLGQRTPVKLRTGHILYIRSYGLDVKEAESVCSD
                     FTHDGRIPEPLRIARLYARSILHAHTSE"
     gene            1523..2449
                     /gene="aglJ"
                     /locus_tag="PK154_03670"
     CDS             1523..2449
                     /gene="aglJ"
                     /locus_tag="PK154_03670"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HPW10195.1"
                     /translation="MSIDKDQVCILIPTLNESPTIGYVIQGYLHLGFSHILVIDGHST
                     DDTRQIAETAGASVILQQGKGKGTAIIEAFSRITLPFILMVDGDGTYLPEDAESIISP
                     LLQGADQVIGDRLIPENRTSFSRMNYFGNQILNRLFKVAHGRYLNDILSGYRAFRLDS
                     IRQMQLHESGFGIETEISAEAVRNGHTIQIVPIAYRKRMGTQTKLNPFHDGLKISTTI
                     YRLAKMSNPLFYFGLIGMIIFCGGLITGIYVIYEWLQQIEHIPLTIFTVLLIIAGFQI
                     FMFGILSDMSISLHREVMREIQSLKEGKDQKK"
     gene            2559..2629
                     /locus_tag="PK154_03675"
     tRNA            2559..2629
                     /locus_tag="PK154_03675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2591..2593,aa:Gly,seq:ccc)
     gene            2640..3320
                     /locus_tag="PK154_03680"
     CDS             2640..3320
                     /locus_tag="PK154_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HPW10196.1"
                     /translation="MITPASDLFAIRIIVENRKGVLRDISTVLARHDANVMMIHQETF
                     DSGPYNGLAELYIEYENGSDHDVMLQELAQLPSVREIKPHEPFSHIYGTRVIIIGGGA
                     QVAQVALGAVNEADRHNIRGERISVDTIPLVGEKTVAQAVDAVARLPRAAILVLAGSL
                     MGGEISHAVDRVKDAGIPVIALRMAGSVPEHADLVVTDPIQAGVFAVMHVSGKAVFDI
                     KRVRGRQF"
     gene            3320..3901
                     /locus_tag="PK154_03685"
     CDS             3320..3901
                     /locus_tag="PK154_03685"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPW10197.1"
                     /translation="MDPVEKAAHVLMHDGIVIYPTDTIYGLGGDAFSDEAILKVYEAK
                     KRPLSQPVSIAVCDCDMIHGVAKINEYAATFIDHFLPGPVTVILPARNSVPPILTGGT
                     GKLGIRLPNHPLALRLISLVGSPITATSANMSGGRDPLTPLECHVAHDYLLDGGILPG
                     TPSTVVDIGERRILRPGALVGEVVSYLSKGEGT"
     gene            3898..4827
                     /locus_tag="PK154_03690"
     CDS             3898..4827
                     /locus_tag="PK154_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HPW10198.1"
                     /translation="MKKPLRLRDFIEDPEGRLYAVAAYDNRERAGCILRYLPDPCGER
                     VDQAGKRYHKLEFEEAFAYILREKPEYADSIQRIPVEDIRKVLKPEDEIGTIARRNTR
                     VARLVSIFDLPERTYGCTGSLLVGLENDRSDIDLVVYGRMFFTARELLRFAIKKGRID
                     PISDDMWENIYRKRNPELSLDEFLIHEKRKWNRGQIENVYFDILFTRSYKALHPLPVT
                     KGPSCGVVTIKATVTDASLAFDSPAVYQIDHEEISKVLSFSHTYSGQAIAGELIEARG
                     ICEEHYGEKWLIVGTTRDARGEYIRSISLLEGG"
     gene            complement(5351..5794)
                     /locus_tag="PK154_03695"
     CDS             complement(5351..5794)
                     /locus_tag="PK154_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10199.1"
                     /translation="MACSVQKNWRICSDSDVPVHSAQYQIHGIVDKLFDEPPFFSIVR
                     SSRAPSSGIYRADRIRIAAYTTCLQEMFGKEIEGGEVEYIPSGITRFCRVEPLDKRRF
                     LRALHEARRIKNGEIPRRPRNPPCERCPEKGRCGPEHGVRLSDLF"
     gene            6157..6453
                     /locus_tag="PK154_03700"
     CDS             6157..6453
                     /locus_tag="PK154_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPW10200.1"
                     /translation="MSQHEIAVNVPQNLDPVYSNMIQIAYKDDEFTFVFLHQIPGVNQ
                     ARAKAIVSITPQHAKNLLAVFSKTFADYESKYGKIEVPSGKTQSESVTTIRGYS"
     gene            6516..6587
                     /locus_tag="PK154_03705"
     tRNA            6516..6587
                     /locus_tag="PK154_03705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6548..6550,aa:Thr,seq:cgt)
     gene            complement(6888..7055)
                     /locus_tag="PK154_03710"
     CDS             complement(6888..7055)
                     /locus_tag="PK154_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HPW10201.1"
                     /translation="MYKSNFGGPRNDGPREMTKVVCSDCGKECEVPFKPTEGRPVYCR
                     ECLPKHRRPRF"
     gene            7738..8109
                     /locus_tag="PK154_03715"
     CDS             7738..8109
                     /locus_tag="PK154_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10202.1"
                     /translation="MRSMVKEWSEKNDMERWKAVSELRTIGRPAVEYLVINLWDSDKR
                     VRMAAVDALGAIGDQRAYDHLVKMLDDPDHDVRFASVIALGKLGDMRAVEPLSRACHD
                     PNCYVRTIAAETLEKFRLQIS"
     gene            8234..9037
                     /locus_tag="PK154_03720"
     CDS             8234..9037
                     /locus_tag="PK154_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW10203.1"
                     /translation="MDQSLKQRLLLKCREMGIPLIGVADVRRWENPPFQPWMPKEFFP
                     HSLVPEAESVIVIGLPVQLPVLETSPSIYYREMYNVLNSLLDQYSYRIAEYLNNEGHP
                     SVFLPRDGYGSIDVLLERPLAFFSHRHAAFCAGLGTFGVNNMILTPEYGPRVRFGSII
                     TSASLPPDPMRDDDLCTRCMRCVRLCPSSALSKEDYPEGITDKTACARWSASLHRQYI
                     SPCGICIKVCPVGSDRTFYRREDPGIYEESAPGSPYQQIWDHVRRYGGK"
     gene            complement(9631..10572)
                     /gene="uvsE"
                     /locus_tag="PK154_03725"
     CDS             complement(9631..10572)
                     /gene="uvsE"
                     /locus_tag="PK154_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HPW10204.1"
                     /translation="MQLSVGDMRIGYPCINRRLDCTSAKKFRLASFNDERFFATVSSN
                     LSCFRQILAWNVTQDILFFRISSDIIPFASHPVCRAPWQEHFSEELGRIGHSIRDEGI
                     RISMHPDQFIILNSPDERIVSRSVDELAYHATLLDLMELGTQAKIQLHVGGRYGDSQE
                     SIARFVRVYDTIDPAIRRHLVIENDETRFSATDCLEISEQTGIPVLLDVFHHACYNNG
                     EETGDMLDATGRTWGADDGIPMVDYSSQHPAKRPGGHAEHIDLSDFREFLELSQPFDR
                     DIMLEIKDKETSALAAIALLQKDPRFERAIGKRVRSG"
     gene            10917..11564
                     /locus_tag="PK154_03730"
     CDS             10917..11564
                     /locus_tag="PK154_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPW10205.1"
                     /translation="MPGEHPNKAPEDTGRPSINELMLLMVDQMRATAQMVRSDEAESF
                     FSELLNAGKIYVAGAGRSGLVARAFAMRLMHIGFESYVIGETITPALSPGDTLVVFSG
                     SGETNSVFDVLETARDLGGRICLITATPVSRMSRLAHSRVILCGSQPSDDSLPTQYEV
                     RQITGQYRSVSSSFAPFGTLFETAALIFSDAIISALIEEKHCSLDEARTRLSNVQ"
BASE COUNT         2968 a         2907 c         2823 g         3026 t
ORIGIN      
        1 cctccttatc gatctccctt ccttctctcc tggtttaatc ggcacgccat ccttcctcca
       61 gatcctcggc atatatcaca acgatgaact agggcgcctg aattatgatt cccgcatcat
      121 ccttatcaat aatgggtcgg attctttggg gaaccagaat cttaccgcgg aattttaccg
      181 gaatggtata aaaatcccgg ctgtggttga gaccatgaat ggcgatctgt tcatctccac
      241 ccgacactac ggcatccaga cgatgggggg gcttggctgt tcagggccca cctggctccc
      301 aaaagagaaa atagccctgg atttcagcga tggaaccttt catcctggtg acacgatacg
      361 aatggatgtg ttttcaaaga cttcagcgac cttgatctca cgccacacgt tcatcgcttg
      421 atgatcctgc ttcagaataa aaaaccgaat ccgcgctttt catcagatcg gtatgtggac
      481 acaggatatc atcttatatc atctaaaaat caggaatgct atctgaccga caggcacggg
      541 aaaagtgggg aagttactct gatcccgctt cctgatctgg ttctgtcggg ggggtagtcc
      601 acaccagcca gagaaccagg gcaatgcaaa tgatcatcga gagaacgaac acgatcaggc
      661 tgaaaatatt ctcagcaaac tgcatggata caggaatgtt caaagcacca agaataagtg
      721 ctatcccaaa gaagacaaaa aaaccaacaa caatgccaag aattaccccg gcgataatcc
      781 ttaatgctga aggttgtgag cgaggatttt tcatgttttt tactgtggtc ctcagttctt
      841 aataacagta tccagcggtc ggtgagttct tttcggaaaa ggaagatggt atcttggcat
      901 gacaagtgag gacagttcat tcagaggtgt gtgcatgtaa aattgaccgg gcataaagtc
      961 gtgcaatcct tagcggttct ggtattctgc cgtcgtgggt aaaatctgaa cagaccgatt
     1021 ccgcctcttt tacatccaac ccatatgacc tgatatagag aatatgacct gtcctgagct
     1081 tcacaggagt tctttgtcca agactcctgt aggcagaaat acgcccttca tcacccggaa
     1141 aatggtggat tatattttcg attagtccct cggagtgctt atacgtcgcg atgatcgttc
     1201 cgcgtcctgt ttcctggaaa atgcggagcg gatcaatgat attgtaccat gatatgacag
     1261 cccccccaac cataatgaag ttgatatctt ccctggaaaa attccggtaa agatctatta
     1321 ccgaatccgt tgcatccatg ccacctaccg tggtgcaggt atacgaaaag ccatcaagcc
     1381 tgagatcttt tctcatcacc acaccagcaa ggatcgaatt cttccgggaa gagaaactct
     1441 cagcaatacc caggacccgg agccctttct tggcgaaatg catggaagca cttatgacag
     1501 gggatttgaa aacaatatcc caatgagtat tgacaaggat caggtatgta tccttattcc
     1561 caccctgaat gagtctccta ccattggcta tgtgattcag ggatatctgc atctgggatt
     1621 ttcccatatc ctcgtcatcg atggtcacag tactgatgat acacgccaga tcgcggaaac
     1681 tgcgggggca agcgtcatac tgcagcaggg gaagggaaag ggaaccgcga tcatcgaggc
     1741 attttcgcgg ataaccctcc cttttattct catggtagac ggtgatggaa cctatctccc
     1801 tgaagatgct gaatcaatca taagccccct gttgcaaggt gctgaccagg tgattggtga
     1861 ccgtctgata cccgagaacc ggacctcctt ctcgcgaatg aattatttcg gtaatcagat
     1921 tttaaaccgg ctctttaagg tggctcatgg tcggtacctc aatgatattc tctcgggata
     1981 cagggctttc aggcttgatt cgatccgtca gatgcagctt cacgaatccg gattcggcat
     2041 agagaccgag atctctgcag aagcggtcag gaacgggcac actattcaga tcgtaccgat
     2101 tgcgtacagg aaaaggatgg gaacgcagac aaagctaaat ccgtttcatg acggattgaa
     2161 gatttccacc accatctacc ggctagctaa aatgagcaat ccgctttttt acttcggttt
     2221 gatcggaatg atcatattct gcggcggttt gattactgga atctatgtaa tctatgaatg
     2281 gctgcaacag attgagcata tcccgttgac catttttacc gtacttctca tcattgcggg
     2341 attccagatt ttcatgttcg ggatcctgag cgacatgagc atctctcttc atcgggaagt
     2401 catgcgcgag atccagtccc tgaaagaggg aaaggaccag aaaaagtgaa tgttatgttc
     2461 tctctctaaa tgagagagaa cggtacggat atctctctcg gggctccggc agcgaaattt
     2521 tatcactggg agaaacatac gattatacac tttattgggc gatagtagtc tagcggcagg
     2581 acagaggctt cccaagcctt tagcccgggt tcgatccccg gctatcgcat acactggcta
     2641 tgattacccc tgcaagcgac ctttttgcaa tacgcattat tgttgaaaac aggaagggcg
     2701 tcctgcgtga tatttcaacg gtacttgcac ggcatgatgc caacgtgatg atgatccacc
     2761 aggagacttt cgattccggc ccgtacaacg gtctcgctga gttgtatatc gagtacgaaa
     2821 acggaagtga tcatgacgtg atgctccagg aactcgctca gcttccatcc gtgcgggaaa
     2881 taaaaccaca tgagccgttc agccatattt atgggacccg ggttatcatc attggtggag
     2941 gggcacaggt ggcccaggtg gcccttggcg cagtgaacga agctgatcgc cataatatac
     3001 gaggtgagcg tatctcggtt gataccattc cccttgtcgg ggagaaaaca gtggcccagg
     3061 ctgtcgatgc ggtagccagg ctcccccggg cagcgatcct cgtccttgcc ggttcactca
     3121 tgggcggtga gatttcccat gcggttgaca gggtgaaaga tgcaggaatt cccgtgattg
     3181 cacttcgtat ggcaggcagc gttccagaac atgctgattt ggtagtcact gatcccatcc
     3241 aggcaggagt atttgcagtg atgcacgtca gtggcaaggc cgttttcgat atcaagcgag
     3301 tgagagggcg gcaattttaa tggaccccgt cgagaaagcg gcacacgtgc tgatgcacga
     3361 tggcattgtt atctatccga cagatacgat ctacgggctt ggtggcgatg ctttttcgga
     3421 tgaagcgatt ctcaaagtgt acgaagcaaa gaaacggcct ctctcgcagc cggtatccat
     3481 tgccgtctgt gattgcgata tgatccacgg agttgcaaaa atcaacgagt atgccgccac
     3541 gttcatcgat cattttcttc ccggtcctgt tactgtcatt cttcccgcca gaaattccgt
     3601 tccgccaata ctgactggag ggacagggaa gcttggaatt cgccttccca atcatcctct
     3661 tgctctccgc ttgatctcac tggtcggcag tcctatcact gcaacaagtg ccaacatgag
     3721 tggtggcagg gatcccctca cacccttaga gtgccatgtt gcacatgatt atcttctcga
     3781 cggaggcata cttccgggaa cccccagcac ggttgtcgat atcggcgaga ggcggatact
     3841 cagacctgga gcgctggtag gagaggtagt cagctacctt tccaagggtg aaggcacatg
     3901 aaaaaaccgc tccgtttgcg cgattttatc gaagatcctg aggggaggct ctatgcggtt
     3961 gctgcctatg ataaccggga gagggcaggt tgcattctcc gctacctccc tgatccctgt
     4021 ggggaacggg ttgatcaggc aggaaagcga taccataagc tcgaatttga ggaagcattt
     4081 gcatatattt taagagaaaa accggaatat gcggattcta tccagcgcat accagtcgaa
     4141 gatatccgga aagtgctgaa accggaagat gaaatcggga cgatcgcccg gagaaacacc
     4201 cgtgttgcca gactggtcag tatcttcgac cttcctgaaa ggacctatgg ctgcactggt
     4261 tcactgcttg ttgggctcga gaatgaccgg tctgatattg acctcgttgt gtacgggcgt
     4321 atgttcttta ctgcaaggga actgctccgg tttgccatca agaaagggcg aatcgatcct
     4381 atcagcgacg atatgtggga gaacatctac cggaaaagaa atccggaatt atcccttgat
     4441 gaatttctca ttcatgagaa gaggaaatgg aaccggggtc agattgagaa cgtctatttt
     4501 gacatcctct ttacgcgttc ctacaaggcg cttcatcctc tccccgtaac aaaaggacct
     4561 tcctgtgggg tagtcaccat caaggcaaca gtcaccgatg cctcacttgc atttgacagc
     4621 cctgcggttt atcagataga tcatgaggag atatcaaaag tcctctcgtt ctcgcatacc
     4681 tatagcgggc aggcaattgc cggcgaactg atagaagcac ggggtatctg cgaggagcat
     4741 tacggggaga aatggctcat tgtcgggaca acacgggatg caagaggcga atatatccgt
     4801 tcaatcagtc ttctggaagg aggctgacat gaatttccgg caaaaaaatc agtgtcctct
     4861 ttttccataa cagcgaaagt gtattctacc ggtagtttct tcagatcgga caccggtatt
     4921 tccgccagcg ctgccacccc tcaaagaaag aagaacgatt gagatatcca tcagcccgct
     4981 cctcccgtgt gaaggggacc caattcttgt aaaacaggtg ttttatgacc tcatcctcaa
     5041 tgctctgaac ttttcatgac ccagcgacgc agcacaggta gagatcggtt cgatgaggcg
     5101 tgattcaaag acggtattct ttgtaaaaga caacggagtt ggtttcaaca tgaactttca
     5161 gcacagctct cctttctgtt cagcggcttc atgatgacaa ggaggatgaa gggacgggaa
     5221 ttggatggca atcgccaacc gctttgtcca tcgggatggt ggaacgatct tggctgaaag
     5281 caacgtggat aatggcgctg gtccctattt gacacttgaa caaacacagt cacagggaaa
     5341 ttaattatga tcagaacaga tctgaaagcc ggactccatg ttcaggtcca caacgccctt
     5401 tttccgggca tctctcgcag ggggggtttc tcggtctccg tgggatttca ccgtttttaa
     5461 tccgccgggc ctcatgaagg gcccgtaaaa atctcctctt atcgagcggt tcgactctgc
     5521 agaaccgggt tatgccggaa gggatatatt ccacctcacc tccttcaatc tcctttccaa
     5581 acatctcctg caggcatgtg gtatatgctg caatcctgat ccgatcagca cgataaatcc
     5641 ccgatgaagg tgccctcgag gatcggacta tggaaaaaaa cgggggttca tcgaagagct
     5701 tgtcaacaat accgtggatc tggtattgtg ctgaatgaac tggcacatca gaatcagaac
     5761 atatccgcca gttcttttgt acactgcatg ccattatgca ctcatcgaaa aattcacggc
     5821 cgtcagggat tttctctctc tgaatgagca tgatctccga ccatatctct tccctgtcag
     5881 tatcagtccc taggtaatat gatacctgtt ttgctacggt atatcgtggg gattcagcta
     5941 tgccggattt cctcccaaga aagaacctca ccggacagat ggctgtggtg acaacggatg
     6001 aaatactgag cggagagatc actggtttca atcatgctgt atgcagcgcg tcttatgtaa
     6061 aactgctgat ctgaaattaa tcccttccaa ggccttaccg tctggccgga tgatcgtttg
     6121 ccaagtctta atcttggagc ggtcgatcta cactgtatgt cccagcacga gattgcggtc
     6181 aatgttcccc agaacctcga tccggtctac agcaatatga tccagatagc gtacaaggat
     6241 gacgaattta ccttcgtctt tctccaccag atccccgggg taaaccaggc acgtgcaaaa
     6301 gcgatcgtga gcatcacccc ccagcatgca aagaacctgc tcgccgtgtt ctccaagacg
     6361 ttcgcggatt acgaatcgaa atacggaaag atcgaggtcc cttcggggaa aacccagagc
     6421 gagagcgtta cgaccatccg gggatattct taaacccccg tccctcccct cccgttacta
     6481 ttaatatttt ccacgtctac attgtgaggt atactgccgc cgtggcttag cggtatagcg
     6541 gctgattcgt aatcagcagg tcgagggttc aagtccctcc ggcggctctt cttttctggt
     6601 tgagcgttgg tactagctga gaagaaggag atattcctca attatcctct ctggtactga
     6661 ttggatatcc ctgatctgcc ctgcctgaaa ccatttcatc agagtatcat cgtccaatgg
     6721 aaaagagctg tatccgccca ttatcaatcc tcaatgctgt taatatccag ttcccagtca
     6781 tgagaggagt taattgcgca gagttgctaa aagtctcgga tctcttccat gccagtgtct
     6841 gctaatgcga tctgcaatgg ataataagac cagaaaaaag aaaaagatta aaaccggggg
     6901 cgccggtgct tggggaggca ctctctgcaa tagacgggtc ttccctcggt cggcttaaac
     6961 ggtacttcgc attcctttcc acagtctgaa cagactactt ttgtcatctc gcgggggccg
     7021 tcatttctcg ggccgccaaa atttgattta tacatggttt tactcaaaca gttgaaaact
     7081 gtctttaact atacaccact caagaatata aatattacta tcgtcccctc cgggcctggc
     7141 atgcacccga ttgcatatca tttatctggt tggttggtcc gggtggcagg atgaacgaga
     7201 ggtggtatca gtttcttcgc ctttgagatt tattggaaaa aaaggtaggt atttcttgga
     7261 gtggtttgtg atgagaaaaa tgatccggag atatccccca tgcatgattc tgtccggtat
     7321 tctttcgctt atgtagtggt cagggatcac aggagccgga ccaacattac cttcttttcc
     7381 cttactgaaa gaaatctctt tgaaattccc cgatagctgc tgtcacgaac agttccttct
     7441 gaataatcac cgacgtaata ccggatatct tcatctgaaa catcccaaat ttatccattc
     7501 ctctatgagc ccccgttttc ttgaccccca cctattttgc agccacatgt gcagggcagc
     7561 caggaaaatg aaccggttcg atgagggaca acgggttatt ttttatgatt ttgtctcata
     7621 atctgctttg catatattta tgctctaata aatatatcgt aaaaacaatt tttccaaata
     7681 acctcgggat ttgaatacgg tttaagattt aaagctgaat tgaaagggga gttatgtatg
     7741 aggtcgatgg ttaaagaatg gtccgaaaaa aacgatatgg aacgatggaa ggctgtctcc
     7801 gagctcagga caatagggcg tcccgctgtg gaataccttg ttatcaacct ctgggatagt
     7861 gataaacggg ttcgcatggc ggccgttgat gctcttggag ctatcggtga tcagcgagcc
     7921 tatgatcacc tcgtgaaaat gcttgatgat ccagatcatg acgtacggtt cgccagcgtc
     7981 atcgctcttg gcaagctggg tgatatgcgg gcagttgaac ctctttcaag ggcgtgccat
     8041 gacccgaact gctacgtcag gacgattgca gcggagacgc tggaaaaatt caggttgcag
     8101 atatcataac taattaaaaa aaacctggcg aaatattttc acgaatgcac aaaggatcat
     8161 tttctctcgt tttctccctt ggtaagcgct ccatgatcct ttttgtatag gaatacgtac
     8221 taatcaggca ggtatggatc aaagcctcaa acaacgtctc cttctcaaat gcagggagat
     8281 gggcatcccc ctcatcgggg tagctgatgt caggcgctgg gagaatcccc cttttcagcc
     8341 ctggatgccg aaggagttct tcccccattc tctcgttcca gaagctgaat cggtgattgt
     8401 cattggtctt cctgtccaac tcccggttct tgagacatcc ccctccattt actaccgtga
     8461 gatgtataac gtgctgaatt ccttattaga ccagtacagc tatcgtatcg ccgagtatct
     8521 caacaatgaa ggacatccat ctgtttttct cccgagggac gggtatggaa gcatcgatgt
     8581 tcttctcgag aggccccttg cattcttctc tcaccggcac gctgcattct gtgcaggtct
     8641 cggtacattc ggagtaaaca atatgatcct cactcctgag tatggaccga gggtcagatt
     8701 tggatctatc attacctcag cctccctccc gccagatcct atgagagatg atgatctctg
     8761 cacaaggtgc atgcgttgtg tgcggttgtg cccctcttct gccctctcaa aagaggatta
     8821 tcccgaaggg atcactgata aaaccgcctg tgcacgctgg agtgcctccc ttcatcggca
     8881 gtacatctct ccctgcggca tctgcatcaa ggtatgtccg gttgggagcg acaggacctt
     8941 ttaccgccgg gaagatccgg gaatatacga agagtcagct cccgggagcc cgtaccagca
     9001 gatctgggat catgtccggc gttacggtgg gaaataaggg gagagatcca tctccaaggt
     9061 tatgcagata ctctcccggg cagtcatctc atctcgttca tagtgaaaca tcccttttta
     9121 gttcatacca tgaaccagac ccggttaccc attttttaac ttcatgcttc ccagtgcaga
     9181 gacatgccgc gaatcatact acaaaggctc ttcccaatgg gaacacacaa agggtttaga
     9241 gaccagaccg gtacattttt ttcggatcca gaaagacgat cccaatggcc gtttggcttg
     9301 ggtctggaag aaaatggaaa ttcgttcgcc ttcttttagg gagatggatg atgcctaccc
     9361 atagaacgag tgcccaaatc cggattcagt ggttcctctt tcgatctgct ctccaccggg
     9421 cagttcgatt ctgggagtgg cacctttcat aagagatcct cttttctctc aactccctcc
     9481 tttgagcaga aatccctcca cctcatgtat gcgacggtat attcctgtat aaacagctga
     9541 atataagaaa aagcagctgg tatcgtgaac acgtacattc ccaacataaa gaacgtcggg
     9601 gtccggagca tttcgcagag aaaggactca tcatcctgac cttaccctct tcccaatcgc
     9661 tcgttcgaat cttggatcct tctgtagcag agctattgct gcaagggcac tggtctcttt
     9721 gtccttgatc tcgagcatga tatccctatc aaacggctgc gagagttcga gaaactcccg
     9781 gaaatcagaa agatctatat gttctgcatg tccaccgggc cgctttgccg gatgctggga
     9841 gctgtaatcc accatgggta tcccgtcatc tgcaccccat gtccttcccg tagcatcgag
     9901 catatctccg gtctcctcgc cattgttgta acatgcatga tggaatacat cgagaagtac
     9961 aggaattccc gtctgttcgg aaatttccag acaatctgtt gcactgaacc gtgtttcatc
    10021 attttcgata acaagatgcc gtctgatagc cggatcgatt gtgtcatata ccctgacaaa
    10081 acgggcgata ctttcctggg aatcaccata gcgacccccg acatgaagct ggattttggc
    10141 ctgtgtgccg agctccatca ggtccagcag ggttgcatgg taggcaagtt catcaacact
    10201 gcgggaaacg atacgttcat caggggaatt gagaatgata aactggtcag ggtgcatcga
    10261 gatacggata ccctcatccc gaatggaatg cccgatacgc cccagttctt cggaaaaatg
    10321 ctcctgccat ggtgccctgc ataccggatg cgatgcaaac gggatgatat cggatgagat
    10381 gcgaaaaaag agtatatcct gtgtcacatt ccatgcaagt atctgtcgaa agcaggagag
    10441 gttgctcgag actgttgcaa aaaatctctc gtcattaaaa gaggccagcc ggaacttttt
    10501 ggcactcgtg caatccaggc gcctgttgat gcagggatac ccgatacgca tatcacctac
    10561 tgagagctgc attggaataa gcaggtttgg ttcggggaaa tacaggttgt gattcctggg
    10621 atgttgtggc tgatacctgt tctttgcgtc gtctatatcc ctttttctcg atagtgagaa
    10681 ggaagagatg gggtatctgc acttcaggta tcattgttat gagaattttt ttcaccacag
    10741 agaaaaatcg ggtatattat tcatcttcaa atagcggggg cagtatcctt gaaaagaaag
    10801 tgccatatcc tgccttcttc cctcaaaggg tatccgtgag aatcccgatg tgcagaattg
    10861 aagcgattcc ataacgtttc gttagagatt taatcatcca tgatcactac atgactatgc
    10921 cgggtgaaca tcccaataaa gccccggaag ataccgggag gccctccatc aacgaactca
    10981 tgctcctgat ggtcgaccag atgagagcaa cggcacagat ggtgcggtcc gatgaagctg
    11041 aatcgttctt ttcggagctc ctcaacgccg ggaagatata cgtagcaggc gccggcagat
    11101 ccggcctcgt agctagggcc tttgccatgc gcctgatgca tatcggtttt gaatcgtatg
    11161 ttatcggaga gaccatcacc cctgcattat ccccgggaga tacccttgtg gtcttctcgg
    11221 gatctggtga gaccaactcg gtttttgatg ttctcgagac agcaagggat cttgggggac
    11281 gaatctgcct cattaccgca acccctgtct cacgcatgtc acggcttgca cattcccgtg
    11341 tcatcctctg cgggagccag ccctctgatg acagcctgcc gacccagtat gaagttcgcc
    11401 agataaccgg tcagtaccgc tccgtctcct cttctttcgc accattcggt accctttttg
    11461 agactgctgc cctgatcttt tccgatgcga tcatctcggc gctgatcgag gaaaagcatt
    11521 gcagtctgga cgaggcgaga acacgcttgt caaatgtcca gtaataccta cccgctcaaa
    11581 agtcatgtca ctcattttaa tcaccataca attccgcctt tctctccttc gttcgtgccg
    11641 gactggttgc ctaaaaataa catgattcct atgagggcca caagcataaa agatgaaaat
    11701 gccgggggca ttttcatata aacc
//
LOCUS       DAOOCO010000041        11555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5228_length_11555_cov_4.201478, whole genome shotgun sequence.
ACCESSION   DAOOCO010000041 DAOOCO010000000
VERSION     DAOOCO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_11555_cov_4.201478"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PK154_03735"
     CDS             complement(<1..1270)
                     /locus_tag="PK154_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPW10206.1"
                     /translation="MKSRYRWWVPLLLLLFLLVLAQPGTGTSDDSAQNGAGMSLSPIN
                     PLFTRYLNDFRSRLDLPSAPGNALGLVPATVDLSHLTGTQISDFSTLASTDTSNTAPV
                     FSIRDSTSSSEITSYALPGSYDLRTQGRVNGVQDQGECGSCWAFSTFASLESGLKPES
                     WDFSENNMRNSHGYDLSSCQGGNAQMATAYLARWSGPLKESSDPYTGISKSASSVTAP
                     NSASVKHIQEVLFIPARSGSLDNQNLKKALTERGAVYSTIRWEESSYRPATASYYYSG
                     KSQENHAITIVGWDDTYDRSHFASTPPGDGAFIVKNSWGSDWGMNGYFYVSYYDTVIG
                     RDNAFFSAEDKGNYDQIYQYDPLGWVVSYGDNSDTAYFANVFTSLSAEDLAAVSFYTS
                     SANSKYQVSVYTGVQSSPVSGLLEYSQSGTI"
     gene            complement(1787..2620)
                     /locus_tag="PK154_03740"
     CDS             complement(1787..2620)
                     /locus_tag="PK154_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10207.1"
                     /translation="MILLVGTVASAEETAVQPQLPHAFYGSVEVAGSPANTGISVEAA
                     GIGVRSNVTGNPVDTLPDGTYGKANFTSQKLIVQGNIAAGTSLHFYVGGLLAEVYDVA
                     AGGPWKETYPFEAGGETELNIRVASQPAAGQTRVPTPTQTAGTVTNNQAGTSSLSSGT
                     TSSGSAQGGATLPQVPASPGQQTTAVTTQQVPSGTIQPGTAGQGQGMEEVTTLVTGPA
                     PAHDPVSLAPTGTMPMYVAGAILLILIIAGGTYYFVSLNGKGTEETTSDQAKEPEEKK
                     D"
     gene            complement(2735..>3687)
                     /locus_tag="PK154_03745"
     CDS             complement(2735..>3687)
                     /locus_tag="PK154_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10208.1"
                     /translation="VGGTAPTTVPTTVPTTVPTTVPTTVPSTIPTTVPTTVPTTVPTT
                     VPTTVPTTVPTTEIPAFPYYISATAGPNGAITPSGSVSVLSGADQSFTIVPATGYTID
                     QILVDGTPVAKTPVYTFTDVAADHTIAVSFREGSPEYFTSELGNGWNLFSTPILLGSG
                     YQYLENIFPSSSLGNIDVILGWNGSAWFIPGYHYELAPLHALYVKVDDSATAYLSPSD
                     AITVPPVRSLVKGWNLIGPAPDYQEGGFSARPVEDTLVTAYGDQENPGYLIVISPGVN
                     QPGWSYVRDAMSVDLLPYKGYWVFMENPETLAGFSTTPIF"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3788..4242)
                     /locus_tag="PK154_03750"
     CDS             complement(<3788..4242)
                     /locus_tag="PK154_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10209.1"
                     /translation="MKSHRKTGWFSLLGLLLILAIVTISPAVAAPILPHTFYGNVTIG
                     SQPAPVNTHITASVTGGGGDLVTTIVGEYGIHDFNTLEVQPSGEDATITGGAPISFFI
                     NGVRAEVREGGKSTWSASYPFASGVDTNLDLRVGGTAPTTVPTTVPTTVP"
     gene            complement(4314..7700)
                     /locus_tag="PK154_03755"
     CDS             complement(4314..7700)
                     /locus_tag="PK154_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPW10210.1"
                     /translation="MTRWPCIGKSRVATAILVVLCCMLLFQACSAADCGTDPDIKDVK
                     LYVDCDYEFYISFHSDAGLQADTNGFWVFFDKAIEIDPTLAEECVQIKWEGHSNKPAA
                     DVAVYGTDELPVFAPANASQAVKIWISESIPMNKDVTIWFKCGLSAPCDEPDCPFGDC
                     ITCDELMCYDFTVWVANDLQRCPQISTRYVKIIASSGPGGHVTYPPTGESAAPDFEKM
                     FKVGSTPTYLITGESHIIDKIVIEPLCEGVPDPEDIIYKDCEDCTIIDKHGSVQATYT
                     FDELHCSYKITATFIKKPVYGYLKYQLPLSECGDVVKVKVWGTDKLQPMLDFADDLYI
                     GGNSAVFNLEAWDDPSTVLWEYGGSGDVDTASGVIPEVVVKSAPVKNPETFMVTYEDT
                     NPQTPTQHATVTILTNGQWVWAPSMPDDVKDIINVEAQEPAWDLVTQNYLDCNPEFEG
                     LIGMVIEADDSKVYTETIEIDTPGVLLKNKEGTAPVIDAKGLTPKKADCDDSDKSGAV
                     YLSSGSTGIEGFTIKNSKTNGVLVWLCSEKCEKATILDSIKGPGCNEKTGCKEVDCDN
                     IEVPCCQGRINIVKNDIVNNCENGIYVNDAVVLISENKIHGNIDDGIDAGCLFCGVET
                     IDPAAITHSPACSEIILNEIYHNGHFPETGVAPKGEWVVEKAGTKYFTSDPTACGKYP
                     GWTDAGIQIRCVGDRCGEPCSECRPNSDEPTNPEDLQPCGPGPCAAGTCCSGTCPAIL
                     APDWEGCGGCNMTLYIVHNNVTDNFHAGVYLMEGATQGGQIIIQANQIHENGVFGLLT
                     EAAIPSRIDFKWNDIWCNKYWGVKNLACCDLVAKENYWGSPGGPSAGPAPIVDCIDCR
                     CHELDQRSNALGNGDEVSHRVHYDPWLYRSAMCMLDGSCTNIRIYGSQLPLQCGWNTL
                     SVPVTLADEANTIPKIATLGKFITRENIVVVYKWDAEDDVWVNVGASGEEIIPGQGYY
                     IKMKSESMFPVLYNAGPSPGLSAISLESGWNLIGAPWGIDREDGCCDLGDQGRWGVAV
                     ADLEDDEAFKQVTDALESIKEGNGGTKGVAIIVSPSVPGQYEAWSASVTSGFWDPVKN
                     TQEMATGDAYWVFMANPATYAGFEITPFYFPAETIHTIHA"
     gene            complement(8138..9187)
                     /locus_tag="PK154_03760"
     CDS             complement(8138..9187)
                     /locus_tag="PK154_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPW10211.1"
                     /translation="MRTRKSYRDLKSEVWDRNICAGCGACVAVCPADAICFGEEGITS
                     CPSNIGYCKEAHDEVPCGACYAACPRKEEPGEKGLGSYQSIMTAKAAFDVPKKQSGGA
                     VTALLVNALDEGLIDAVVTVSEDRWTLKPSSVVITSSEEIIHQAGSRYNWWVPLVAAL
                     KTAVIERKFRKIAVIGVPCVVQALHAIRTSDNDLLRPYGRSIRLVIGLFCTESFDYTE
                     LVERKLKREYQVDTWKVERLDVRKGNLEVTMKDQPPLLLPMSELETTVRQGCHSCLDL
                     TGVCSDISAGAVGSGPGYTTLIIRNDVGRMFQESAVKNKKLVIGPQADIAAIDRLASF
                     KVRKNRQKKEPQKTV"
     gene            complement(9193..9948)
                     /locus_tag="PK154_03765"
     CDS             complement(9193..9948)
                     /locus_tag="PK154_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10212.1"
                     /translation="MTELQDAIRIDASGMHYTPLNRAIRAAVTSGVRRIHLDNIMGQR
                     FIADGLRGDVEIVINGVPGGDLGMFMSGPTCIVHGNCEHAPGNTMDAGTIVIHGNAGD
                     AVAHSMRGGMIFVRDDIGYRGGIHMKQYQEKRPLLIVGGSARAFLGEYMAGGLILVLG
                     RKRRSPLLERGIGSGIHGGKIIIRGDVPDWNLGVGARVDKMTEEERGEINPLIEKFAC
                     YFGLDPQPFLTDHYSIITPSSPRPFANKYTWEV"
     gene            complement(9945..11453)
                     /locus_tag="PK154_03770"
     CDS             complement(9945..11453)
                     /locus_tag="PK154_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPW10213.1"
                     /translation="MSIGSVPLRYRISVDRDQCMLCGRCVDSCPYGVFRKEGEQIHVN
                     SRVCVACHRCISMCPRDAISLVERPVDYRSHPIWTPQVREAIYNQARTGKIILSGMGN
                     ALDYPVIFDRLVLDACQVTNPSIDPLREPMELRTYLGKKPSHLNFKTVEGDTVLQTIL
                     SPNLMLETPIMIGHMSYGAISLNAQQSLARAVTECGTFMGTGEGGLHESLYPFQDHMI
                     VQVASGRFGVDINYLERGAAIEIKIGQGAKPGIGGHLPGEKVCADVSCTRMIPEGSDA
                     ISPAPHHDIYSIEDLKQLVRSLKEATEWKKPVFVKIAAVHNSAAIAAGIARSSADAVV
                     IDGFRGGTGAAPRVFRDHVGIPIEAAVASVDAKLRSQGIRNEVSIIASGGIRESADVA
                     KVIALGADAVYIGTAALVAMGCRVCGTCYKGLCPWGIATQRPDLVARLDPDYASVNVA
                     NLIHAWTLELSELMGAAGINSIESLRGNRDRLRGYLLDENIMKVLDIKTVGA"
BASE COUNT         2569 a         3022 c         2941 g         2923 t
ORIGIN      
        1 cgatcgtccc ggactgtgaa tattccaaca aacccgagac aggactactc tgtactccgg
       61 tatagaccga tacctggtat tttgaatttg ctgaggaggt atagaagctc accgcagcaa
      121 gatcttctgc cgacagtgaa gtgaaaacgt tggcaaagta ggcagtatcg ctgttgtccc
      181 cgtaggagac tacccaccca agcggatcgt actggtagat ctggtcatag ttccccttgt
      241 cttctgcact gaagaacgcg ttatcccttc caataacggt atcataatat gacacgtaga
      301 aatacccgtt cataccccag tcgctgcccc atgaattctt tacgataaaa gcaccatcac
      361 caggtggtgt cgaggcaaag tgagagcggt cataggtgtc atcccaaccg acaatggtta
      421 tcgcgtgatt ctcctgggat tttcctgaat aataataact tgccgttgcg gggcggtaag
      481 aggattcttc ccatctgatg gtagaataca ctgcacctct ctctgtgagt gcctttttca
      541 ggttctgatt atcgagcgat ccgctccttg ccgggatgaa gaggacttcc tgaatatgtt
      601 tcaccgatgc ggaattggga gcggtgacgg atgaagcact tttcgagatt ccggtatagg
      661 ggtcgctcga ttccttcagg gggccgctcc atcgtgccag gtatgcagtt gccatctgag
      721 catttccccc ctggcacgaa gagaggtcat atccatgcga atttcgcatg ttattctcag
      781 agaaatccca tgattcaggc ttcaggcctg attcaagaga ggcaaatgtt gaaaacgccc
      841 agcaactccc gcactctccc tgatcctgca ccccgttcac cctgccctgg gttctcaggt
      901 catagctgcc cggcaaagcg tatgaggtga tttcacttga cgatgtcgaa tcacgtatcg
      961 agaagacagg tgcagtattg ctggtatcgg tggaggccag ggtgctgaaa tcagaaatct
     1021 gtgtacctgt aaggtgggag agatcgactg tggcaggaac aagacccagc gcatttccgg
     1081 gagcagaggg gagatccagc cgggacctga aatcgttgag ataccgggta aataaaggat
     1141 tgatgggaga gagggacatt cccgcaccat tttgtgccga atcatcagag gtcccggtcc
     1201 caggttgtgc gaggaccagc aggaacaaca gtagcagcag cggtacccac catcggtatc
     1261 ggctcttcat tttatctcca tggtattgta aggtataatc gacaatttgt tatattcttt
     1321 ttactaaagt gtctcgtttt actaccagta tccccattgt cagttatctc ttttccggtc
     1381 gtgtataccc tgatgcgggc ctttgatgac catgagatac atcttttgat ctgatatcgg
     1441 gctgatccgg actcgttttt tctacgatcc aatcctgccc gttttaattg gagtattccc
     1501 tgtccggttt ataaaatcat aggatagttc cggtaggaca acaaagctcc atggcattat
     1561 gcttcgtacc catgatgcct gggtaatttt tggtgggatg agacgaggtg aaaccggggt
     1621 cccggatctt acggatagtc ttaaaaagag taccctgatt cacttgaggg acccgagcaa
     1681 atcagatgca aatgccggaa acattttcat gtttactatc gatgaaggct attcgttcga
     1741 acataaagaa tgaaaatgcc ggaggcattt tcattggaaa aaggtatcag tctttcttct
     1801 cttcgggttc ttttgcctga tcggatgttg tctcttcagt gccttttccg ttcagggaga
     1861 cgaagtaata tgttcctccg gcgataatca ggatgagcaa tatcgctccg gcaacataca
     1921 ttggcatagt tcccgttgga gcaagtgaga cgggatcatg tgcaggggcg ggccctgtca
     1981 ccagggtggt cacctcctcc ataccctgtc cctgcccggc tgttcctggc tgtatagttc
     2041 ctgaagggac ctgctgggtg gtgaccgcag ttgtttgctg acccggagat gcaggaacct
     2101 ggggaagcgt agcccctccc tgggcagaac ctgatgaggt agtgcctgat gacaatgagg
     2161 aggttcccgc ctggttattc gtcacggttc ccgcagtctg ggttggcgta ggcaccctgg
     2221 tctgtccggc agccggctga gaagcgacac ggatgttcaa ctccgtctct cctccggctt
     2281 caaaaggata ggtctccttc catggtccgc cggctgcaac atcataaacc tcggccagga
     2341 gtccaccgac atagaaatgg agggatgtcc ctgctgctat gttcccctga acaatgagtt
     2401 tctgtgatgt aaaattcgcc ttcccatagg ttccatccgg cagcgtgtcc accgggtttc
     2461 ctgtgacgtt tgaccgaacc ccaatgccag ccgcttcaac agaaattcct gtattcgcag
     2521 gacttcctgc gacctctacc gatccataaa aagcatgggg gagctgtggc tggacggcgg
     2581 tctcttcagc agaagcaaca gtacccacga gtaggatcat ggatactgcg agcaggactg
     2641 atagaatgct gattctctgt ggtttcatgg tatcacggca gtctgtaaaa aagggtaatt
     2701 ccttctttta ttttactctt ttcacatctt cccttcagaa aatcggggta gtggagaatc
     2761 cggcaagagt ttccggattt tccataaata cccagtatcc cttgtaagga aggaggtcaa
     2821 ctgacatcgc atcgcgcaca tatgaccagc cgggctggtt aacacccggg cttataacga
     2881 taaggtagcc gggattttcc tggtccccgt aagcggtgac cagggtatct tctacaggtc
     2941 gtgctgaaaa tccaccctcc tgatagtcag gtgcgggccc gatgaggttc catcctttca
     3001 caagggagcg gacgggaggc accgtgattg catcggaggg agagagatat gcggtggccg
     3061 aatcgtcgac tttgacatag agggcatgga gtggcgccag ctcatagtgg taaccaggga
     3121 tgaaccaggc ggatccattc cacccgagga tcacatcgat gttcccgagt gaactggagg
     3181 ggaatatatt ctcaaggtac tggtaacctg atccgagcag gataggcgtt gagaagagat
     3241 tccatccgtt tccgagttct gaggtaaagt attcaggtga cccttctctg aaggagaccg
     3301 caatggtatg gtctgccgcc acatcagtga aggtgtaaac cggggttttc gcaaccgggg
     3361 ttccatcgac aaggatctga tcgatggtgt atccggttgc cggtacgatc gtgaaggact
     3421 gatcagcacc actgagcacg gaaacagagc ctgacggggt gatggcacca tttggtcctg
     3481 cggtagcact gatgtaatag gggaaagccg gtatttcggt agttggaact gtggttggca
     3541 cagtggttgg tacggtagtc gggacggttg ttggcacagt ggttggtacg gtagtcggga
     3601 tggttgaagg taccgttgtt gggactgtgg taggtacggt agtcgggacg gttgttggaa
     3661 cggttgtcgg ggctgttcct cctacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnggt acggtagtcg ggacggttgt tggaacggtt gtcggggctg ttcctcctac
     3841 acgaagatcg aggttggtat ccacaccgct cgcaaatgga tagctggccg accaggtgga
     3901 tttcccacct tcccgtacct cggccctgac accgttgatg aagaacgata tgggggctcc
     3961 tccggtgatc gtagcatcct ccccggaggg ttgtacctca agggtattga aatcgtgaat
     4021 tccgtattcc ccaactatcg tagtgaccaa atctccccca ccgcctgtta cacttgcagt
     4081 gatgtgagtg ttgacagggg ccggttgcga tccgatcgta acattcccat aaaacgtatg
     4141 tggaagtatc ggagctgcta ctgctggtga tatcgtaacg attgcaagga tcagcagcag
     4201 accgagcaga gagaaccacc ccgtctttct atgagatttc attgatcaat cctcctgccc
     4261 ggaaagaggg gaggtgttac ccctctctcg atgagcaaag tctatccttc tttctatgca
     4321 tggattgtgt ggatggtctc ggccgggaag tagaacgggg taatctcaaa tcctgcgtaa
     4381 gttgcagggt ttgccatgaa tacccagtat gcatccccgg tagccatttc ctgggtgttc
     4441 ttgaccgggt cccagaaccc gctggtcaca gatgcactcc atgcctcgta ctggccgggt
     4501 actgagggac ttacgatgat agcaactccc ttggtgccac cgttgccttc cttgatcgat
     4561 tcaagggcat ccgtcacctg cttgaaggct tcatcgtcct caaggtcagc cacagcgact
     4621 ccccaccggc cctggtcgcc gagatcacag cagccgtcct cacggtcaat accccacggg
     4681 gcaccgatca ggttccagcc cgattcaagg gagatcgccg agagtcctgg tgagggtccg
     4741 gcgttgtaga gtaccgggaa catcgactcg gatttcatct tgatgtagta tccctgtccc
     4801 gggatgatct cttcgccgct ggctccaacg ttcacccata cgtcatcttc tgcatcccac
     4861 ttgtagacca ctacgatatt ttctcgggtg atgaactttc cgagggttgc aatcttgggt
     4921 atggtgtttg cttcatcagc cagggttacc ggtaccgaca gggtgttcca gccacactgg
     4981 agcgggagct gtgaaccata tatccggatg tttgtgcagg aaccgtcaag catacacatt
     5041 gccgagcggt agagccatgg gtcgtagtga acacggtggc tcacttcgtc accattgccg
     5101 agtgcattgc tccgctgatc cagttcgtgg cagcgacagt cgatgcagtc aacgatcggt
     5161 gcgggtccgg cactcgggcc accggggctg ccccagtagt tctccttggc aaccaggtca
     5221 cagcaggcga ggttcttgac accccagtac ttgttgcacc agatgtcgtt ccacttgaag
     5281 tcgatcctgc tgggtattgc tgcttctgtc agaagaccaa agaccccatt ttcatggatc
     5341 tggtttgcct gaatgatgat ctgccctccc tgggtggcgc cttccataag gtagactccg
     5401 gcatggaagt tatcggtcac gttgttgtga acgatataga gggtcatgtt gcatccgcca
     5461 cagccttccc aatctggcgc aagaattgcc gggcaggtgc cagaacaaca cgtacctgct
     5521 gcacagggtc caggtccaca gggttgcaga tcctcagggt tggttggttc gtcactgtta
     5581 ggccggcatt cagagcaggg ttctccgcac ctgtctccga cacaccggat ctggatacct
     5641 gcatcagtcc accctgggta cttgccgcag gcagttggat cgctggtgaa gtactttgtt
     5701 ccggcctttt caactaccca ttcacccttg ggagctaccc cggtttctgg gaagtgtccg
     5761 ttgtggtaaa tctcgtttaa tatgatctcg ctgcatgcgg ggctgtgggt gatcgctgca
     5821 ggatcaatgg tctcaacgcc gcagaagagg cagccggcat cgataccgtc atcgatgttg
     5881 ccatggatct tgttctcgga gatgagaacg actgcgtcgt tgacgtagat accattctcg
     5941 cagttgttga caatatcgtt cttgacgata ttgatcctgc cctggcagca tgggacctca
     6001 atattatcac aatctacttc cttgcaaccc gttttctcat tacagccggg cccttttata
     6061 ctgtcaagaa ttgtggcttt ttcgcatttc tcgctacaga gccacaccaa tactccgttt
     6121 gtcttggagt tcttgatagt gaatccttca attcctgtgc tgcctgatga gaggtatacg
     6181 gcaccggact tgtcagaatc gtcacaatca gctttcttcg gggtcagtcc cttcgcatcg
     6241 ataaccggtg ctgttccttc cttgttcttc aggaggacgc cgggggtgtc gatctcgatt
     6301 gtctcggtgt ataccttgct gtcatccgct tcgataacca tgccgataag cccttcgaat
     6361 tctgggttgc agtcaaggta attctgggtg acgaggtccc atgcaggttc ctgtgcctca
     6421 acgttgatga tgtccttgac atcatcaggc atggatggtg cccacaccca ctggccattt
     6481 gtcaggatcg ttactgttgc atgctgggtt ggagtctggg gattggtatc ctcatacgtg
     6541 accatgaagg tctcggggtt tttaactggt gctgatttca ccacaacttc aggaataact
     6601 ccggatgctg tatcaacatc gccgcttcct ccatactccc agagaacggt agaaggatca
     6661 tcccaagctt ccaggttgaa gactgcggaa tttcctccta tatagaggtc atcggcaaag
     6721 tcgagcatag gctggagctt atcggttccc cataccttga ccttgacgac atccccgcat
     6781 tcactcagcg ggagctggta tttgaggtat ccatacaccg gcttctttat gaacgtcgcg
     6841 gtgatcttgt aagagcagtg gagctcatcg aaggtgtagg ttgcctggac actaccgtgc
     6901 ttgtcgataa tggtgcaatc ttcacaatcc ttatagataa tgtcttccgg atccggtacg
     6961 ccttcacaga gcggctcgat tacaatcttg tcgatgatgt gactctctcc cgtgatcagg
     7021 tatgttgggg ttgacccgac tttgaacatc ttctcaaaat caggggcagc actctctccc
     7081 gtcgggggat aggttacatg tccacctggg ccggaggatg caatgatctt cacatacctc
     7141 gtgcttatct gcgggcaccg ctggaggtcg tttgcaaccc agacggtgaa gtcatagcac
     7201 atcagctcat cgcaggtaat gcagtctcca aaaggacaat ccggttcgtc acagggagca
     7261 gaaagaccgc atttgaacca gatcgtgaca tccttgttca tcgggatgct ctctgatatc
     7321 cagatcttta ccgcttgaga cgcatttgct ggtgcaaaca cgggcagttc atctgtaccg
     7381 tataccgcaa catccgctgc gggtttgtta gaatgtcctt cccatttgat ctgcacacat
     7441 tcttctgcga gggtcgggtc tatctcgatc gctttgtcaa aaaagaccca gaacccgttt
     7501 gtatctgcct ggagccctgc gtctgaatgg aatgagatgt agaattcata atcgcaatcg
     7561 acgtacagtt tgacatcctt gatgtcagga tctgtaccac aatctgctgc tgaacaagcc
     7621 tggaaaagaa gcatgcagca gagcacaacc agtatggctg ttgctacgcg tgatttacca
     7681 atacatggcc accttgtcat atctatcctc ctttctgatg acatcccggc ctgccggccc
     7741 tatagccacc acgctcctgc cggcagcacc atcactgctg acagttgtag agaccggttc
     7801 accgtgctgt cacgaataat ctgcaatttt ttctggtatt cccaccagtt ctggcgagaa
     7861 cccggacatt ttctctctgc tgttcccttt tttcgatcac agggtatgca gtgctccctt
     7921 gtccgaaagg ggataaaaca ccgtatgaaa taatactata taaaagttat tcacatttat
     7981 ttgaaaaatg ttgagaaaat ggggatgatt gggggtatgg agggataatt tatgcaaatt
     8041 tgcattttgt ataatatggg atattattaa tataaccaat tggataaatc attgaactct
     8101 ccggtgtaat ctcaaataat tttcatttat ttttaaatta aactgttttt tgtggctctt
     8161 ttttttgcct gtttttccgg accttgaaag aagcaagcct atctatagcg gcaatgtcag
     8221 cttggggccc gattaccagt tttttatttt tcaccgcgct ttcctgaaac atccttccta
     8281 cgtcgtttct tatgatgagg gtggtgtatc ccggcccgga tcccaccgca ccggcagaga
     8341 tgtcagaaca gactcccgtg agatccaggc atgagtggca tccctgtctg accgtggttt
     8401 ccagttcgct catgggaagg aggagagggg gctggtcctt catggtgacc tcaaggtttc
     8461 ccttccgcac gtcaagccgt tccaccttcc aggtatcaac ctgatactct ctttttaatt
     8521 ttctctcgac aagctcggtg taatcgaacg actcggtaca gaataacccg atgacgagtc
     8581 ggatagatct gccatatggc ctgagaaggt cattatcact ggtcctgatc gcatgaagtg
     8641 cctgtactac gcaggggacc ccaatgaccg caatctttct gaacttcctc tcgatcactg
     8701 ctgtctttaa ggcagctaca aggggaaccc accagttgta tctgcttcct gcctgatgaa
     8761 tgatctcttc gcttgaggtg atgacgaccg atgagggctt cagggtccac cggtcttcac
     8821 tcacggtaac aacagcatcg atcagcccct catcgagggc atttacgagc agggccgtta
     8881 ccgcaccccc gctctgtttt ttcgggacat caaacgccgc ctttgcggtc atgattgact
     8941 gataggaacc aagacctttt tcaccaggct cttctttcct ggggcaggct gcataacagg
     9001 caccacaggg gacctcgtca tgtgcctctt tgcagtaccc aatatttgag ggacaggagg
     9061 ttattccctc ctctccaaag cagatagcat ctgcagggca gaccgcaaca caggctccgc
     9121 accctgcaca gatattgcga tcccagactt cagatttgag atcacgatag ctcttccttg
     9181 ttctcatcag catcatacct cccaggtgta tttattagca aagggacggg gactggaagg
     9241 ggtgatgatg gaataatggt cggtcaggaa tggctgggga tccaggccga aataacaggc
     9301 gaatttttct atcagcggat taatctcgcc cctctcttct tccgtcatct tatcgaccct
     9361 ggcaccaacg ccgagattcc aatccgggac atccccccgg atgatgatct tgccgccatg
     9421 gataccggaa ccaattcccc tttcaaggag gggggatctc cttttcctgc caagaaccag
     9481 gatgagtccg ccggccatgt attccccgag aaatgccctt gccgacccac caacgataag
     9541 gaggggacgt ttctcctgat actgtttcat gtgaatcccg ccccggtacc cgatgtcatc
     9601 acgtacaaaa atcatcccgc cacgcattga atgggctacc gcatcccctg cattcccatg
     9661 gatcacaatt gtccccgcat ccatcgtgtt tccaggtgca tgttcgcagt tcccgtgtac
     9721 aatgcaggtc gggccgctca tgaacatccc gagatccccc ccgggcacac cgttgataac
     9781 aatctcaaca tcgccgcgga gcccgtctgc tataaaccgc tgccccatga tattatccag
     9841 atggatcctg cggacaccgg aggtaacagc agcacggata gctctgttga gtggtgtgta
     9901 atgcataccg gatgcatcga tccgaatagc atcctgcaat tccgtcatgc ccccacagtc
     9961 ttgatatcga ggactttcat gatgttttca tcaagcaggt atcctcgcag tcgatcacgg
    10021 ttgccccgca atgattcgat actgttgata cctgcagcac ccatcagttc tgacagttca
    10081 agagtccagg catgaataag gtttgccaca tttaccgatg cataatcggg atcgagcctt
    10141 gcaacaagat ccggccgctg ggttgctatt ccccatgggc agagtccttt gtaacaggtc
    10201 ccgcataccc tgcatcccat cgcgacaaga gctgcagtcc ctatgtatac ggcatcggca
    10261 ccaagggcga tcaccttggc cacgtccgca ctttcacgaa ttccaccgct tgcaatgatg
    10321 gagacttcgt tacggatgcc ctgcgagcgg agcttggcat caacacttgc aaccgctgcc
    10381 tcgatgggaa tgccaacgtg gtcccggaaa acacggggtg cggcaccagt tccccccctg
    10441 aaaccatcaa tcacaacggc atcagctgaa gatcgggcta tccctgctgc gatagcagca
    10501 gaattatgaa cggcggcaat ctttacaaaa accggttttt tccattcggt cgcttccttc
    10561 aggctccgga cgagctgctt cagatcttca atactataaa tatcatggtg aggtgccggg
    10621 ctaatcgcat cacttccctc cgggatcatc ctcgtgcatg agacgtctgc acagaccttc
    10681 tcaccgggaa gatgtcctcc gattcccggt tttgccccct ggcctatttt gatctcgatt
    10741 gccgcacccc tctcaaggta attgatgtcc acaccaaagc ggccggaagc aacctggacg
    10801 atcatgtgat cctggaatgg gtagagtgat tcgtgcagac ccccttcacc agtacccata
    10861 aaggttccac actcagtcac agcacgggcc agtgactgct gggcattgag actgatagca
    10921 ccataactca tgtgaccgat catgatgggc gtctcgagca tcaggttcgg agagagtatc
    10981 gtctggagta ccgtgtctcc ctctacagtt ttgaaattca ggtgtgatgg cttcttcccg
    11041 agataggtcc tcagttccat cggctcgcga agcggatcaa tactgggatt agttacctgg
    11101 caggcatcaa gaaccagccg atcgaagatg acagggtaat ccagtgcatt tcccattccg
    11161 gagagtatga tcttcccggt ccgagcctga ttgtaaattg cctcccttac ctgcggggtc
    11221 cagatgggat ggctccggta atccaccgga cgttcaacaa ggctgattgc atcccggggg
    11281 cacatcgaga tacaccggtg gcaggcaacg cacaccctgg aattgacatg gatctgttca
    11341 ccttccttcc ggaacacccc gtatggacaa ctatcaacac atcgtccgca aagcatgcac
    11401 tggtcacggt ccacgctgat gcggtatcgg agcgggacgc tcccgatgct cattactgtg
    11461 ccctcccaat gatcggttct ccagcccgcg gcatggtgat gtcacccacc ccgggatcca
    11521 aggtccgtat cgccgcttct tcgctggata tgtag
//
LOCUS       DAOOCO010000042        11455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5289_length_11455_cov_5.259737, whole genome shotgun sequence.
ACCESSION   DAOOCO010000042 DAOOCO010000000
VERSION     DAOOCO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_11455_cov_5.259737"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..620)
                     /locus_tag="PK154_03775"
     CDS             complement(6..620)
                     /locus_tag="PK154_03775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPW10214.1"
                     /translation="MEKEAVRQLNAHITTCTRCRLSLTRTNAVPGEGPVPSELFFIGE
                     APGRNEDLTGRPFVGRAGTVLEDLLATIGLQRDRVFITSIVKCRPPENRVPKKDEIAA
                     CEGYLQKQIDLAFPRILIPMGRLASELVFSHFGLPNIPIGRIHGQPVHAGPSCRDLTI
                     FPVYHPAAVTHNPPLKGTIFEDFQALGRIIEENGLKFRQVKRVK"
     gene            complement(735..1622)
                     /locus_tag="PK154_03780"
     CDS             complement(735..1622)
                     /locus_tag="PK154_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10215.1"
                     /translation="MTAGEEKILSEKVIDAQGQTYHLVLTGRDLGGQYPGMKIYTLQV
                     VSGGNVIACFRTNTYEYPPSSPISASGVIQARSDEWERDLLSDPTRFLEREERAIREP
                     DDLPAHDVLILQGSPRPAGNCSVLATWAQAEADRAERSARVVYLDDLTIRACIGCYQC
                     YNTGTCIFEDDMTEIIRSISHARIIVICTPVFTNTVPGSLKIMIDRCQAYHAEQALTG
                     RKSGKTGLLFSVAGRQGQENFSCITRVIDAFLKNIHIKPVRSVLFDDLDRIGDIRNIP
                     YAEEKVRDAMKKVLKDMDT"
     gene            1735..3015
                     /locus_tag="PK154_03785"
     CDS             1735..3015
                     /locus_tag="PK154_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HPW10216.1"
                     /translation="MDPLPSFFLTLLLVSFLGIRFRIPPIFNLTGGALFLGILIGMPP
                     DQLISVITTGLGRIFALFALIILSGAIIAAILEEQGDIQQIVHDIRNHSANPYTISGL
                     SGYLLALPVACSVTAFIILSPVVKGLGNGEKTRTLLLYCVAIGSLISFGLVYPTPAVI
                     PLVQSLAPDLSPALYDALALPLSILLLFCFLLLFRFWFGSCDKPESMAKGPDDSPQKT
                     VSSSEKRNFRAYAPFIAILLAIPVGLSIGLSHGALIQVIMLAGAATAIMTAAPGIRAA
                     GLQRGTKHAGVIIFDICGAGALAGVILASGFSETALGQITAIIPVLLVPFVLASLIQT
                     AQGSRVVTAVLTAGILAGTQVAEVIHPVALIIMIAAGCFVFSFVTDPFFWIVSRTTGD
                     DFAQVIRRYTIPLALCGAALYLVALGMQLIVSGG"
     gene            complement(3038..4096)
                     /locus_tag="PK154_03790"
     CDS             complement(3038..4096)
                     /locus_tag="PK154_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW10217.1"
                     /translation="MILPSDQGGRPGDIRRQILWYAIMAPSPLNTQPWSALCRDPCGI
                     DLYLEKKRLLSHMDPASRQACVSCGAFIENLDIAAREAGFTAEISLLPSSLPGADVDL
                     GQPLARIELMPDPGIVTDPLFSWLEKRHTNRSIFTNDPIPAEIISSLADAANQPFTTL
                     GFSADPSFRQELGGHITNAVEVTFSAQDRFSEFLSYVRIPPISAHQYPDGYGAREIGL
                     HGIRGWLSLLPLRISPAGRRNGLARGLLLRLARKGAESAAAFGWIATKGNSRHDQIRA
                     GRTYERVHLTAASAGVSLQPMTCVLESYPGMEDHYRRVLDLLGIPDTHTVQMLFRLGY
                     SPSPAQSRRWAVQDFFGR"
     gene            complement(4742..6133)
                     /locus_tag="PK154_03795"
     CDS             complement(4742..6133)
                     /locus_tag="PK154_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW10218.1"
                     /translation="MSDQTNRSSRGVILVIVTIGTFLNPFTGSSINLALPLIGEEFAI
                     DAIMLSWIPAAYLLSSAIFLLPAGMLGDIIGRAKVFSTGTLVYTFASLFAFFTPSASL
                     LLACRFLQGIGGAMLYSTAIAIIAELYPPGERGRAVGINVMAVYGGMTLGPFIGGILT
                     QLFGWRSIFFITVLLGCTVLLFVRRFPAILNENRRGTFDYQGAGLSAVALVLFFVGTS
                     RIPNTDAFVYILLSGLAFLIFYRVESGKASPLFPITLLSSNRIFSFSNLAALINYSAT
                     FAVTFLLSLYLQIVRGMSPATAGSVLLIQPFVQMIIAPVAGRLSDRTDTSKLAAIGLF
                     TSSVGLLGLAFLGMDTALSWVVLFLILLGCGLGLFSTPNTTVVMGSVEKSQYGIASSF
                     LATMRSTGMMLSMGIVMIVFSFMLGSTSITPEMAPGFLASMQLIFLVFFVLTVLGALI
                     SLDKKSPDRSSKA"
     gene            complement(6239..6661)
                     /locus_tag="PK154_03800"
     CDS             complement(6239..6661)
                     /locus_tag="PK154_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW10219.1"
                     /translation="MTVYACCQTNVVSVKPDATVQFVATLMRDKNIGCVVVTDNHQPV
                     GIVTDRDIALRSETLCGDPESALIESIMTRDLKTIRKDTGIFDAIQEMKASGIRRMPI
                     VDQGGRLVGLLTVDDLIRLLAREMADIARIIGKESPAL"
     gene            complement(6728..7510)
                     /locus_tag="PK154_03805"
     CDS             complement(6728..7510)
                     /locus_tag="PK154_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10220.1"
                     /translation="MDTYLIPRISRLSLNVWRRNWDVFFKTYQVNFLPPFIEPVLYLL
                     AIGFGLGMYVGEINGIPYVNYVAPALLAISIMNASFFECTYSSYVRMYYQKTFDAMIA
                     TPLSLDDVITGELLWGATRSLIYSSVMLLVLILFGVVDLPLSLLIIPFSFLAGLLFAC
                     IAMCFTAITPGIDALNYPAFLFITPMFLFSGTFFPLEVLPQTVQVIAITLLPLTQLVI
                     ITRSLTLSSLSPLFLYSLLWIIMVTALCFILSLRLMRRRLIV"
     gene            complement(7518..8426)
                     /locus_tag="PK154_03810"
     CDS             complement(7518..8426)
                     /locus_tag="PK154_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10221.1"
                     /translation="MGGIIRAEHLTKTYGILTAVDSISFSVQQGEMFGFLGPNGAGKT
                     TTMRMIQCISPKTSGELSVFDLDVTTHPREIKGKLGVLPQETNLDPDFTCFENLTNYA
                     RYFEIPKREAANRAEDLLSFVQLEEKRDIPIEKLSGGMKRRLLLARALINNPELLILD
                     EPTIGLDPQGRHLIWEKLKSLQASGNTIVLTTHYLEEAERLCNRLVIMEGGKILVEGT
                     PQDLISKYVGADILEVEHTRPVIDCLDTMHVRYEIFGDIVQVHTDKPREIAKELFECC
                     NPGRVYARQATLEDVFLKLTGRILRE"
     gene            8557..9810
                     /locus_tag="PK154_03815"
     CDS             8557..9810
                     /locus_tag="PK154_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW10222.1"
                     /translation="MPIREIAVSNFKSFRDLQFSPDNFNIIIGSNASGKSNLIQVFKF
                     IRDIAKYGLNNAISLQGGVEYLRNTIIGPAMPFAFRIVYVLEEKRADVIGVKDGLPVY
                     FEPYEIKYEFALTFPDSGESYEISMDLLTVSGNFCHSGASGIHQLGAGTLTLSRVDGS
                     VAYCVCPPEGLELKDQDLFPQYFQKRQIPQKWLIMNLPISVPGIPPIEWIFRGMKVYD
                     FDPRLLKRAVPIMGKTELEKDGSNLAIVIKNILEDPKRKTEFFSLIREMVPFVDEIAV
                     EKFMDMSLFLKLKEIYSRNKFLPASMVSDGTINILALIIALYFENRPVTIIEEPERNI
                     HPFLISKLVEMLKDAASSKQVIVTTHNPEMVKQVDIEDILLISRDAEGFSRLSRPFTR
                     KEIRTFLEHEIGIEDLFIQNLLGME"
     gene            9807..10382
                     /locus_tag="PK154_03820"
     CDS             9807..10382
                     /locus_tag="PK154_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10223.1"
                     /translation="MKERRLFILVEGNDDERFFSRVVKPLFADLYTSVEIVMYACMKN
                     EKVNSFIRSIGEMGHDFIMCADIDQEPNVLAKKRLLNERYDLYANDHFIIIVREIESW
                     YLAGMDERAQHRLGIRRYGSTNHITKEIFNGMIPRHYKSRIAFMGDIITLFSVHEARE
                     RNRSFSFFCHRYRLLPDETDPNTPPPAPETE"
     gene            10521..11012
                     /locus_tag="PK154_03825"
     CDS             10521..11012
                     /locus_tag="PK154_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HPW10224.1"
                     /translation="MKLEMKDSPGQLVAALKPISEVGGNIIAVIHQRDEKAEGDVLGV
                     QIVLELQEQRLGELLSLIKSQGVNVLRLGKERLLYKQTMIMIGHIMHTDLGDTVDRID
                     CTGFAEVTELTMVMPAINEPSSARLVIKAVNKADMEQALGILRGVAKEKNLLVIEPLE
                     ATG"
     gene            11009..>11455
                     /locus_tag="PK154_03830"
     CDS             11009..>11455
                     /locus_tag="PK154_03830"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315144.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPW10225.1"
                     /translation="MKIAILGMGSVGRGLLEMIDGKELGLMVTGIADSKSALMDSDGI
                     HIEEVLMAKRERGYCGDKSLSAADVVERCDYDVLVEVTPTDADSGEPATGFMKTAIGR
                     GKHVVTSNKGPIALHFSTLQSLARKHGVVIRYEATVGGAIPVMHTLE"
BASE COUNT         2803 a         2891 c         3046 g         2715 t
ORIGIN      
        1 tcaggttatt ttactctttt tacctgcctg aatttcagac cgttctcctc tattattctc
       61 ccgagagcct gaaaatcctc aaaaattgtt cctttgaggg ggggattatg ggtcactgca
      121 gcagggtgat agactgggaa tatcgtgaga tccctgcatg atggcccggc atgtaccggc
      181 tgcccgtgaa tacggccaat agggatgttc ggcagcccga agtgggagaa gaccagttcg
      241 gacgcaagcc gtcccatagg gatgagtatc cttggaaatg caaggtcgat ctgtttttgg
      301 agataccctt cacaggccgc tatttcatct ttttttggaa cccggttttc tggtggccgg
      361 catttgacaa tacttgtgat aaataccctg tcccgctgaa gaccgatcgt agcgagcaga
      421 tcttcgagca cggtacctgc acgtccaaca aatggccttc ctgtgagatc ctcatttctc
      481 ccaggagctt ctccgataaa aaacagttcg gagggtaccg ggccttcacc agggacggca
      541 ttggtgcgtg tcagggaaag cctgcaacgg gtacatgtcg tgatgtgagc gttgagttgc
      601 cgcacggcct ccttttccat acatggtatt cgacggtccc agagattatg atattcgttg
      661 gaccgtactg caggttggcc ggaaggatac gcgttgtttc ccttttatcg cgaccgttcc
      721 atcgacaacc ccattcaggt atccatatcc ttcagaactt ttttcatggc atcccggact
      781 ttctcctctg catacgggat gttccggata tccccaatcc tgtcgagatc atcaaataag
      841 acacttctga cgggttttat gtggatattt tttaaaaatg catcaataac acgggtaata
      901 catgagaaat tctcctgtcc ctgacgcccg gccacagaga agaggagccc cgttttccct
      961 gacttccttc cggtaagagc ctgctccgca tgataggcct ggcagcgatc gatcatgatc
     1021 ttaagacttc cggggacggt gttggtgaat acgggggtgc agatgacgat aatccgggca
     1081 tgggagattg atcggattat ctctgtcata tcatcttcaa agatgcaggt gccggtatta
     1141 taacactggt aacatccgat gcaggcccgg atcgtaagat catcgagata aaccacccgt
     1201 gcactcctct cagcacggtc ggcctctgcc tgagcccatg ttgcgaggac actacagtta
     1261 cctgccggtc tcgggctccc ctgcaagatc aggacgtcat gggcagggag atcgtccggc
     1321 tccctgattg ccctctcctc ccgttccagg aaccgggttg gatccgagag cagatctctc
     1381 tcccattcat ctgatctggc ctgaatgaca cctgatgcgc tgatagggga tgaagggggg
     1441 tattcgtagg tattcgtccg gaaacaggcg atgacattcc ctccagacac gacctggagg
     1501 gtatagatct tcatccccgg atactgaccc cccagatccc ggcctgtaag aaccaggtgg
     1561 taggtctgcc cctgtgcatc gatcactttt tcggacagta tcttctcctc accggccgtc
     1621 atttatgaaa ctatcattcg tcccagccat tatgttcttt gtgtcggtcc tggaacgggg
     1681 ctgatctctg caatacccct gtatttatta tcctgaaact aatgagtgaa acatatggat
     1741 cccctcccat cctttttcct gaccctcctc ctcgtctcat ttctcgggat acgattccgg
     1801 atcccgccaa tttttaacct taccgggggg gcactttttc ttggcatcct gatcgggatg
     1861 ccccctgatc agctgatcag cgtgattacg acaggtctgg ggaggatttt tgccctgttt
     1921 gcactcatca tcctctcggg agctatcatt gcagctatcc tcgaggagca gggggatatt
     1981 caacagattg tgcatgatat caggaaccat tcggccaacc cgtatacgat atccggcctc
     2041 tcaggctacc tgctggctct ccctgtggca tgcagtgtta ccgcattcat tatcctatcc
     2101 cctgttgtga aagggctcgg gaatggagaa aagaccagaa cgctgctcct gtattgtgta
     2161 gccataggga gcctcatctc attcggactg gtctatccca ctcctgcggt tatccctctc
     2221 gtccagtctc tcgcccccga tctgtccccc gccttatatg atgccctggc tctccctctc
     2281 tcgattcttc tgctgttctg ttttcttctc ttgttccgtt tctggttcgg atcctgtgac
     2341 aaacctgaga gcatggccaa aggacctgac gactcccccc agaaaaccgt ttcatcctct
     2401 gaaaagagaa acttccgtgc atatgcaccc ttcatcgcga tcctcctcgc gatcccggtc
     2461 ggactttcca ttggcctttc gcacggtgca ctgatacagg tgatcatgct cgcgggtgct
     2521 gctaccgcca ttatgacagc ggctcccgga atccgggcag ccggattgca acgcggaacg
     2581 aagcatgcgg gcgtgattat cttcgacatc tgcggtgcgg gggcccttgc aggagtcatc
     2641 cttgcgagcg gtttttctga aacagcactg ggacagatca ccgcaatcat ccctgttctc
     2701 ctggtgccct ttgtccttgc ttccctgatt cagacagccc agggttccag ggttgttacc
     2761 gcggtactga cagccgggat ccttgcggga actcaggttg ctgaggtgat tcatccggtt
     2821 gcactcatca tcatgatcgc agcagggtgt tttgtattct cgtttgtgac cgatcctttc
     2881 ttctggatag taagccggac taccggggat gacttcgcac aggtgattcg ccggtatacg
     2941 attccgctcg cgctctgtgg agcggccctc tatcttgttg ccctcggaat gcagctgata
     3001 gtgtcaggag ggtgatcccc tggggtgcag cccctcatca tctcccgaaa aaatcctgta
     3061 ctgcccatct tctggattgg gcaggggaag gtgagtatcc aagccggaaa agcatctgga
     3121 cggtgtgcgt gtcagggatg ccgagaagat cgagcaccct cctgtagtgg tcctccatcc
     3181 cgggatagga ctcgaggacg cacgtcatgg gctggaggga cacgcctgcc gaagcagcag
     3241 tgagatgtac tcgctcatag gtgcgtcccg cccgtatctg gtcatgccgg gagttgcctt
     3301 tcgtcgcgat ccagccgaac gcagcggctg attccgcccc ttttcgtgcc aatcggagaa
     3361 gcagtcccct tgctagtcca tttcttcttc ctgccgggct tatccggagg gggaggagtg
     3421 agagccagcc tcgaatccca tgaagtccaa tctccctcgc cccatacccg tccgggtact
     3481 gatgggcgga aatcggggga atccggacat aggaaagaaa ctcggagaag cgatcttggg
     3541 cagagaatgt tacctcaact gcattcgtga tgtgccctcc gagttcctgc cggaatgacg
     3601 gatcggctga gaatcccaga gtggtgaacg gctggttggc tgcatccgcg agtgaggaga
     3661 tgatctctgc cgggatagga tcgtttgtaa agatactcct gtttgtatgg cgtttttcga
     3721 gccatgaaaa gaggggatca gtcacaatcc ctggatcagg catgagctcg atccttgcaa
     3781 gaggctgccc gagatcgaca tcagcacctg gcaacgagga tggcaaaagt gagatctcag
     3841 ccgtgaatcc tgcctcccgt gcagcgatat ccaggttctc gatgaatgcg ccgcaggaga
     3901 cacaggcctg gcgggatgcc ggatccatat gggacaggag acgctttttt tccagataca
     3961 ggtcaatccc gcatgggtcc ctgcagagtg ccgaccaggg ctgggtattc aaggggctgg
     4021 gtgccatgat ggcataccag aggatttgtc tgcggatatc cccgggtctg ccaccctgat
     4081 cggagggaag gatcattgca gttcacactc ctccgggtgg agacaagacc gcacctttgg
     4141 gctgaaagcc gggtgtcatc atgcctctcc aggtatgtca ggtctttgta aagtgccccg
     4201 gtaatgaaaa taaagaccca gtgcgactat cagagtggaa aggctcacca tatttgcacc
     4261 aataacaatc agatcatcga taaagacgcc gtaaaccgcc catagcgcaa ctccaagagt
     4321 aactggacca gggtaaggac actgatgtct ttgaccgatc tcgttctgta catcctgatt
     4381 atctgcggga tgaaaccaaa cattgtcaat acggcggcca cgataccaac aaagaaccag
     4441 aagatcatgg acaatcagct accttttaaa atagtgcgtc cttcagcaaa tgcattgtga
     4501 cctcctcgtt gcactcatcc ccggatcctc atacgatatt ccgtcgccga tttcaaggga
     4561 aggcctccag tattttgacc gtataaaaac gcgtgtttcc atgcgggaat catataattt
     4621 tttcgacttc cggtattgct gcccagatgc attgggggga actcaattct ggtggaggag
     4681 atcgaaaaag gagatgctcc cggagtaagt gggccgtccc aggattcaat cgaagcccac
     4741 atcatgcttt actgctccga tcgggacttt tcttgtccag ggagatgagg gcaccaagaa
     4801 cggtgagcac gaaaaacacg aggaagatca gctgcatgct tgccaggaat ccaggagcca
     4861 tttccggtgt gattgaagta ctgccgagca tgaatgaaaa gacgatcatt acgataccca
     4921 tcgagagcat catccccgtg ctccgcatcg ttgccaggaa tgaggacgcg attccatact
     4981 gactcttttc cacgctcccc ataacgacgg tggtgttggg agttgagaag agccccaggc
     5041 cgcaaccgag caggataagg aagaggacga cccatgaaag cgccgtatcc attccgagga
     5101 aagccaggcc gagaagcccg acagaagagg taaagagacc gatcgctgcc agctttgagg
     5161 tatcggtcct gtcagagagc cttcctgcga ccggcgcgat gatcatctgg acaaagggct
     5221 ggatgaggag gactgatcct gcggtagcgg gggacatacc ccggacaatc tggagataaa
     5281 ggctgagcag gaatgtgact gcgaaggtag cactgtaatt gatgagagcg gcgagatttg
     5341 agaaagagaa gatcctgttt gaagagagta gcgtgatcgg aaaaaggggc gaggcttttc
     5401 cggactccac acgataaaaa atgagaaacg ccaaccctga aagaaggatg tacacgaaag
     5461 catccgtatt ggggattcgg gacgtgccta caaagaagag gacgagggca acagcagaga
     5521 gccccgcacc ctgataatca aatgttcccc gccggttttc gttaaggatt gcagggaacc
     5581 tcctgacaaa aaggaggacc gtgcagccaa ggagaaccgt tataaaaaat atactcctcc
     5641 atccgaagag ctgggtgagg atccccccga tgaaggggcc gagggtcatg cccccgtata
     5701 ccgccatcac gttgatgccg acagcccggc ctctctcccc tggggggtac agttctgcga
     5761 tgattgcgat tgccgtggaa tacagcatcg cccctccgat tccctgaagg aaccggcagg
     5821 caagaaggag ggatgcagaa ggggtgaaaa aggcaaagag tgatgcaaag gtgtacacca
     5881 gggttccggt ggaaaatact ttcgctctcc cgatgatatc gccgagcatt ccggcaggga
     5941 gcaggaagat cgctgaagag agaagatatg ctgccgggat ccaggacagc atgatggcat
     6001 ctatggcaaa ctcctcgccg ataagcggaa gggcaaggtt gatgctcgaa ccggtgaacg
     6061 gattgaggaa cgtgcctatc gttacgatga caaggatgac tccccgcgag ctgcggtttg
     6121 tctgatcaga catcaggtgc tcccctgaac ctggaaataa aaattacggt aatcgtgatg
     6181 aaatcttgca tcagggcttt tattaatctt taccggcaaa tcctgaaaaa tggtcgtatc
     6241 agagagcggg gctctctttt ccgataatac gtgcgatatc agccatttcg cgggcaagga
     6301 gcctgatgag gtcatcaacg gtgagcagtc ccacaagacg gcctccctgg tcaacgatcg
     6361 gcatccttcg gatccctgag gctttcatct cctggatggc atcaaatatt cctgtatctt
     6421 tccgaatagt cttgagatcc cgggtcataa tgctctcgat cagggccgat tcaggatccc
     6481 cacagagtgt ttcactccgg agtgcgatgt ccctgtcggt cacaatcccc actggctggt
     6541 ggttatcggt aaccaccaca cagccgatat tcttgtctct catcagcgtc gctacgaact
     6601 ggacggtggc atcaggtttg acgctcacca cattggtctg gcagcatgca tacacggtca
     6661 taggtagtat cccggttcta agaaaaatga cgtgacagta ttaagttatg atcctgaatc
     6721 ctgcagttca gacgatgagt ctccggcgca taagccgcag agagaggatg aagcagaggg
     6781 cggtcaccat gattatccag agaaggctgt agaggaagag aggggagagg gatgagaggg
     6841 taagtgaacg ggtgatgata acgagctgtg taagcggcag gagggtaata gcaatgacct
     6901 gtactgtctg cggcagcacc tcaagcggga agaaggtccc cgagaagaga aacatggggg
     6961 tgatgaagag gaacgccgga taattgagtg catcgatacc cggggtgatc gcggtaaaac
     7021 acattgcaat gcaggcaaag agcaggcctg cgaggaatga gaacgggata atgaggaggg
     7081 ataacgggag gtcgaccacg ccaaaaagaa tgagaacgag cagcatcact gatgagtaaa
     7141 tgagacttct tgttgcaccc cacaggagtt cacctgttat cacatcatcc agcgagagtg
     7201 gtgtcgcgat catagcatca aacgtcttct ggtaatacat cctcacataa gaagaatagg
     7261 tacattcgaa gaacgacgcg ttcatgatgg agatggcgag caacgcgggg gcgacataat
     7321 ttacataggg aatgccattg atctccccga catacatccc gagtccgaaa ccaattgcaa
     7381 ggagatagag gaccggttct atgaaggggg ggaggaaatt gacctggtag gtcttgaaga
     7441 acacatccca gttccttctc cacacgttga gggacagtcg tgatatccgg ggaatcagat
     7501 aggtatccat gtccccctca ctccctaagg atcctcccag taagtttcag gaacacatcc
     7561 tcaagcgttg cctgtcgtgc atatacccgg ccgggattgc agcattcaaa cagctctttt
     7621 gctatctctc tcggcttatc ggtatgaacc tgaacaatgt ctccgaatat ttcgtatcgc
     7681 acatgcatag tgtccaggca atcgattaca ggacgggtgt gttccacctc aagaatgtca
     7741 gcacccacgt atttcgagat gagatcctgc ggggttcctt caacaaggat cttcccaccc
     7801 tccatgatga ccaaccggtt gcagaggcgc tctgcctcct caagataatg cgtggtgagg
     7861 acgatggtgt ttcccgaagc ctgaagactt ttcagtttct cccagatcag gtgacgaccc
     7921 tgtggatcga gccctatcgt cggctcatct aaaataagga gctccggatt gttgataagg
     7981 gcccgtgcca gaaggagccg tcgtttcata ccgccggata gtttttcaat gggaatatcc
     8041 cgcttctcct cgagctgtac aaaagagagg aggtcttctg cacgattcgc tgcctctcgt
     8101 ttcgggatct caaagtagcg ggcataattc gtaaggttct caaaacaggt gaagtccgga
     8161 tcgagatttg tttcctgagg gagtaccccg agtttcccct tgatctcccg gggatgggtg
     8221 gtgacatcaa ggtcaaagac agagagttcg ccgctcgtct ttggggatat acattggatc
     8281 atcctcatcg ttgtcgtttt cccggcgcca ttcggtccca ggaagccgaa catctctccc
     8341 tgctgtactg aaaagcttat tgaatcgaca gctgtaagta tgccatacgt tttagtgaga
     8401 tgctctgcgc ggattatccc tcccatactc cctctgctat tgagtaggtg ggttcgatcc
     8461 caagaacatt ctttctattg gttattcctg cttccatatc agagatacaa gtcctcggcc
     8521 aaagattatt gtatcacgaa tctgagaagg tttgttatgc caatacgtga gattgcggtc
     8581 agcaatttca aaagtttccg ggatctgcag ttctcaccgg acaattttaa catcatcatc
     8641 ggatcaaatg catccggaaa gtcaaatctt atccaagtct tcaaattcat cagggatatt
     8701 gccaaatacg ggctcaataa tgccatctct ctccagggag gagtggaata cctccggaat
     8761 accattatcg ggccggcaat gcctttcgcc ttcaggatag tctatgttct ggaggagaag
     8821 agggcggatg ttatcggagt gaaggatgga cttcccgtat attttgaacc gtacgagata
     8881 aagtatgaat ttgccctgac ctttcccgac agcggtgaaa gttacgagat ttcaatggat
     8941 ctcctgacag tttcaggaaa tttttgccat tccggagcgt caggaataca tcagcttggt
     9001 gcagggacac tcacgctctc aagggtcgac ggatcagttg catactgcgt ctgcccacct
     9061 gaaggactcg aactgaaaga ccaggatctc tttccccagt atttccagaa gagacagatc
     9121 ccacagaaat ggctgatcat gaacctcccg atatcagttc ccgggatacc ccctatcgaa
     9181 tggatattcc gcgggatgaa ggtatacgat tttgatccca gactcctgaa gcgtgcagtc
     9241 cctatcatgg ggaagaccga acttgagaag gacgggagca atctcgccat cgtgataaag
     9301 aatatccttg aagatccgaa gagaaaaaca gaatttttca gcctgatcag ggagatggtc
     9361 cccttcgttg atgagatcgc ggtggaaaaa ttcatggaca tgtcactgtt cctgaaactg
     9421 aaggaaattt acagcaggaa caaatttctc cccgcatcca tggtctccga tggtaccatc
     9481 aacatccttg ccctcatcat cgcgctctat ttcgagaacc ggccggtcac gatcatcgag
     9541 gagcctgaac ggaatatcca tccgttcctg atctccaagc ttgttgagat gctcaaggat
     9601 gccgcttcat cgaaacaggt cattgtcacc acacacaatc ccgagatggt aaaacaggtc
     9661 gatatcgagg acattttact catttcaagg gatgcagagg gattttcacg actgagccgt
     9721 ccttttacga gaaaagagat acgtacgttt cttgaacatg agatcggtat cgaggatctc
     9781 tttatccaga acctcctggg aatggaatga aggaacggcg cctcttcatt ctggttgagg
     9841 ggaacgacga tgagcggttc ttctcaaggg tagtaaaacc gctttttgct gacctgtaca
     9901 catcggttga gattgtcatg tatgcctgca tgaagaatga gaaggtgaac agtttcatcc
     9961 gcagtatcgg cgaaatgggg catgacttca tcatgtgtgc cgatatcgat caggaaccca
    10021 atgtcctggc aaaaaagcgc cttttaaacg agaggtatga tctttatgca aatgatcatt
    10081 ttatcattat tgtacgggag atcgagagct ggtaccttgc cggcatggat gagcgggcac
    10141 agcatcgcct cggcatcagg aggtatggga gcaccaatca tatcaccaag gagatattca
    10201 atggcatgat cccccgccat tacaaatcaa gaatcgcgtt catgggcgat attatcacct
    10261 tgttctcggt gcatgaggcg agggagagaa accgttcttt ctcttttttc tgtcacagat
    10321 atcgtcttct tccggatgaa actgacccga acactcctcc ccctgcccct gagacagaat
    10381 agtaatcgtt tttttcatga gacggggata tctcccaacc tctttttgcc aagtattggt
    10441 gaaatccggg atgtgagggt aaaaggctta tcactcccgg attcgaatag aggtataggt
    10501 ggtttctggt gcaggtatca atgaaactgg agatgaagga ctctcccggt cagctggtgg
    10561 ccgcgttgaa gccgatatcg gaagttggcg gaaatatcat cgcggtcatc catcagaggg
    10621 atgagaaagc tgaaggagat gtgctgggcg tccagattgt gctcgaactg caggaacaac
    10681 gtctggggga actgctttca ctgataaaga gccagggagt caatgtgctc aggctgggaa
    10741 aagagcggct cctgtataaa cagaccatga tcatgatcgg gcatatcatg cataccgatc
    10801 taggagatac cgtagatagg attgactgca ccgggtttgc agaagtgacc gagctcacca
    10861 tggttatgcc tgccatcaat gaaccctcct ccgcacggct tgtaataaaa gctgtcaaca
    10921 aagctgacat ggaacaggct ctgggcatcc tccgcggagt cgcaaaagag aaaaatcttc
    10981 tcgtcattga gcctctggag gcaacgggat gaagatcgct atcctgggaa tgggatccgt
    11041 cggccgaggc ctccttgaaa tgatcgatgg aaaggagctc gggcttatgg tcaccggtat
    11101 tgcagactca aagagtgccc tcatggactc tgatggtatc catatcgagg aagtcctgat
    11161 ggcaaaacgc gagaggggat actgcggtga caagagcctc tcggcagctg atgttgtcga
    11221 acggtgcgat tacgatgtgc tcgtcgaggt gacaccgact gatgcagatt ctggtgagcc
    11281 tgccaccggt ttcatgaaaa cagccatagg gaggggaaaa catgtggtca cctccaataa
    11341 aggtcccatt gcactgcatt tctccaccct gcagtccctt gcccggaaac atggggttgt
    11401 catacggtat gaagcgacgg tagggggcgc gatcccggtc atgcataccc ttgag
//
LOCUS       DAOOCO010000043        11445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5296_length_11445_cov_4.270149, whole genome shotgun sequence.
ACCESSION   DAOOCO010000043 DAOOCO010000000
VERSION     DAOOCO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_11445_cov_4.270149"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PK154_03835"
     CDS             <1..1239
                     /locus_tag="PK154_03835"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10226.1"
                     /translation="DAHFTVLDSNTWADVSGQGMWLAEDLQTDKTFRFVSFHYPPYSS
                     DSKHFGGWLNIREEWGDELTEGEVMAVFNGHVHAYERFLVDGIQYFVSGTGGGPSYNL
                     ATPRSDYSQNSLEYVLAYLRVTVDPDTGTATADVIRVADISPDLHSITTQYPPNTVFE
                     TVVMTLNTPPILDSMVATMEPTPVNSEITVNAYFRDPSDTHTAVFDWGDGTTSEGVVD
                     EQTGMETYGSVTEAHIYSQPGLYSVSLTLTDRWGETATINCQYVIIYDPDGGQVNGGG
                     TIQVMAGDWSFNPESSGKASFSLSGKYNKGVPSGNINLIFMDAKSQFKAEYLDYMVIG
                     DGTAYLHGYASINKSSPDYEFMLVVQDGKITAGEDTYRLQVWDNLGQNKIFDNAPEAG
                     DRINPDTVPISGGSIVIQMM"
     gene            1718..6160
                     /locus_tag="PK154_03840"
     CDS             1718..6160
                     /locus_tag="PK154_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10227.1"
                     /translation="MKNVWAILLIVIAALVLTAPVIAASSTSLTITKLASDGTTVLDT
                     RTVDYTWMMTNLPVLGDGTTHYYAQGPVFIDDPDPVIEQQLRWNPDEDNNIDKDMGAV
                     KGTNLKDLCDLVGGMNAGETIQVTANDGFTKYFAYKNIYEYSTREGPMVIAWYQNGNY
                     PDTGYSDGMRLVWMADDLVNPNGNHVFGNYDWYLAADEAYWYYYVSGSEKYPTTSGLS
                     VTFVSDITIYSDDPAPSMDVLFDGTVVLTPGETFDKVAYNTGTTYTVSRTTPMGALDV
                     AATTAGFTYDVTDKNYATSGALLLDNVGTYLRDKTNGIYWYAYVNDVYKDGYNNPAGG
                     LNLIELNDGDKVEFYYAAGIADPADLAAVKAAATAAVKIVADTTPGPVMDVLFDGTVV
                     LTPGETFDKVAYNTGTTYTVSRTTPMGALDVAATTAGFTYDVTDKNYATSGALLLDNV
                     GTYLRDKTNGIYWYAYVNDVYKDGYNNPAGGMNLIELNDGDKVEFYYAAGITDPADLA
                     AVKATATAAVKTVASTGVAPTDWSIVIDGAKTQTVTKSYFENGLACAASSHQVFWTDD
                     DDNVWGGVPLWLLVAMVDDDPDVGPDHFNLNDDLAAQHYEVKVISSDGWSAVFDSSAV
                     ARSSDYLVANTLNGEPLIYGGEKPSWPLHLKGAAVLGGQQVGGIVRIELNNLPEPPAG
                     WTLEMLGEVGDVITQEEFEEGLACPVADHYVEWMDASGNVWSGMPLWLVVGAVDDIEL
                     TDSYTFNDEVAAAGYTVKITAIDGFNKTFDSAVITRNNDYLVANKINGEPLDESMFPL
                     RMVGAGVTKPDGSLGGSAVGKIAKIEILELQTPPAAPGSYNLAVKGKITDVFSQAEVE
                     EGLACPSSGHFVTWTQEIKDDSGTIIETHEWSGIPLWFLAGWVDDRVPHEFNAVQASA
                     GYKITVRASDGYAKDFPSADVAWSNDYIVATTKDGAPLPDGKWPLQLVGDALAKADGT
                     LGGMSVSKIAEIELTEFGVPTEIPKVHIVKYAADGMTVVNEVTLDYTQMQEQFMVVGD
                     GKTQYRYQGVTMDPEDIWGETDETKGGYKISNAVKGTRVADLVSLVGGMGEGTDIVFV
                     ASDGFKTTLPYTCIFTTPDIQARQGDAILAWYADGKYVPGYADGMRLFFMPEDTIYGQ
                     WDMHETIPAGYWHYYYQAYNAADPIYGKYAPGILYPSCAGLSAKYITEIKVYTTPEST
                     WDLHLDGTRVGGIEHTIAKGYFEAALACQMGANHDASYTDSSGNVWTGMPLWFLQGFV
                     DDADQHSNNAYNEALALEGYDIIVMASDGYNKTFSSVDTVRSTGYIVANTLNGYHIPE
                     SDASWPLRLVGDDVPKKDNVKKVSDIILNFKPNVSMAYPSQESLWPLNKNYVNVTIEG
                     VADPYDEVTISIISITSDEPTTAGGKNQAPDADPSCIGTDTARIRAERIGNGNGRVYE
                     ISFVATDSMGASMEGTVNVFVPHDQGMVSECIDDGQIYDATLII"
     gene            complement(6386..6904)
                     /locus_tag="PK154_03845"
     CDS             complement(6386..6904)
                     /locus_tag="PK154_03845"
                     /inference="COORDINATES: protein motif:HMM:NF039541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HPW10228.1"
                     /translation="MNVLFLPKDGVALYHELSTSETSREALRFYRPVETPSGVAITVA
                     SLGSALSLVADLRWYVKRYMRGVLFEISPALYCSYGLAREVYFNRAVTLGDTWEFRLV
                     YRMKNGQVVREEQVVPGRERPLGGTPESPEIEVWCTEDEYHCGISVPTGGDDGDGVGE
                     GGGTSEGGDEGS"
     gene            complement(7176..7862)
                     /locus_tag="PK154_03850"
     CDS             complement(7176..7862)
                     /locus_tag="PK154_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10229.1"
                     /translation="MLEAQNLHKSFSAHQVLKGVSISVNPGEIQVLIGLNGCGKTTLQ
                     RILTGILDPDQGTVSIGSVDITGLPPEDRRIGYVPQHTALFKHLSVRENILYPLQNGR
                     GSEETFHKIVKLLSLEPYLDWKPHKLSGGYRARAALARALVSAPRVMLLDEPITEVDR
                     AKKEYILPLFRDVLLQLHIPVLYITHDPWEAGQIGTSFSVMKDGVVNRIDSPEEAFAL
                     IREQARSNGE"
     gene            complement(7837..8640)
                     /locus_tag="PK154_03855"
     CDS             complement(7837..8640)
                     /locus_tag="PK154_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10230.1"
                     /translation="MAGSCMRKVSIWEVLFVPLFFLLIVYPILAIIGNVTIEGMIKTF
                     DDPIFWDSVKNTLITAFFAALLGVLMALGFGYFYIFHPGTIIYKIADFMNDLPIALPH
                     TVAGLALLLAFGRNMFGFIGPTGLAFTLIAVVLAMFFVSYPLAARTIASAAEEIDKEQ
                     ISVARTLGDTPPKAYRRVVIPLLGEAIFSGFVLSFSRSISEFAAVIMFGGNVPGMTQV
                     LASYVFTKVEEGEVSMAVTASAFCILLSLMLVIVLRLRGRLHARSAESA"
     gene            complement(8618..9388)
                     /gene="modA"
                     /locus_tag="PK154_03860"
     CDS             complement(8618..9388)
                     /gene="modA"
                     /locus_tag="PK154_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW10231.1"
                     /translation="MERPHRFIGIIGLFLLVGFLLLSAGCTQTEEAPLNVYVAAGLKK
                     PMDVVIAKYTNATGITVIPNYGPSGGLYTQISQGQPCDLYYSADYNLIEKLEAEGKVS
                     EAEKFMKEFVVMTVSKTGQEKGITTAANLTSENIVVAVADPSAPVGVYSENVLKNLGL
                     WDQLNALGNIKTRPSTVNQVALMVQSDEVDAGFTYSSTAVLYDLVTTEKYGYDLSEEI
                     VFGDAIIKGGNEAGAEAFRDFVRENSVEFTQYGWELYA"
     gene            complement(10136..11293)
                     /locus_tag="PK154_03865"
     CDS             complement(10136..11293)
                     /locus_tag="PK154_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HPW10232.1"
                     /translation="MEETILTPSGESELSWQLKAQDLESQLLDLKVRNDELARDNAQL
                     KRENNQLKRMPLFVAVVIDLLGKGEVYLRQQGNNQEYITHVNDELFSQLKPGTKVAVN
                     NALSIVRIVGNIFDSRVRVMELEESPDMTFDHVGGLEKEIEEVREAVEYPLTKPEIFA
                     RIGVEPPKGILLYGPPGTGKTLIAKAVAHEAKATFIRMSGSELVHKYIGEGAQLVREL
                     FSLAREKAPTIVFIDEIDAVGSTRTNDGTSGSAEVQRTLMQLLAEMDGFDNRGDVRIM
                     AATNRVDMLDPALLRPGRFDRVIAIPLPDKAGRKAILGIHSRKMTLIDVDLDRIVDMT
                     ENATGADLQAVCREAGMMAVRREADGVTEEDFEKAVRKVKAEVVTDLRMYT"
BASE COUNT         2760 a         3075 c         3051 g         2559 t
ORIGIN      
        1 gatgcgcatt tcacggtgct ggatagcaat acctgggctg atgtatccgg tcaggggatg
       61 tggcttgccg aagatctgca gaccgataaa acgttcagat tcgtctcttt tcactatcct
      121 ccctacagtt cagactccaa gcactttggt ggctggctca atatacggga agaatgggga
      181 gacgagctca ccgagggaga ggttatggct gtcttcaacg ggcatgtaca cgcttatgag
      241 cggttcctcg tagatggcat ccagtatttt gtttccggaa caggaggtgg cccctcatac
      301 aaccttgcaa ccccacggag tgactattcc cagaacagcc tggaatatgt gctggcttac
      361 ctccgcgtga cggtagatcc ggatactggg acggccacag cagacgtgat cagggtcgcc
      421 gatatttcac cggatcttca tagcataacc acacagtacc cgccaaatac ggtctttgag
      481 acggtggtga tgaccctgaa tactcctcca atccttgatt ccatggtcgc aaccatggaa
      541 cctacaccgg taaatagcga aattacggta aacgcatact tccgggatcc ttccgatacc
      601 cacactgctg tatttgactg gggagatgga accacctccg aaggggtagt tgatgaacag
      661 accgggatgg aaacgtatgg ctctgtgaca gaagcccata tatactcaca accaggcctg
      721 tattcagtga gtctgaccct tacagaccgt tggggtgaaa ccgcaacaat caactgccag
      781 tatgtgatca tctatgaccc tgacggcgga caagtgaatg gtggtggcac catccaggta
      841 atggcaggag attggtcttt taaccctgaa tcaagtggaa aagcgagctt cagcctgagt
      901 ggcaaataca acaagggagt cccgtcaggg aacattaatt tgatttttat ggatgccaag
      961 tcgcagttca aggccgagta cctggattac atggtcatag gggatggtac cgcctacctc
     1021 catggatatg cgagcatcaa taagagttcg cccgattatg agtttatgct tgtagtgcag
     1081 gacggtaaaa taacagcggg ggaggataca taccggctcc aggtctggga taatctcggt
     1141 caaaacaaaa tattcgacaa cgctcctgaa gcgggtgaca ggatcaatcc agacactgtt
     1201 cccattagcg gcggatcgat tgtcatccag atgatgtgaa tgaaataatc cgtcacaata
     1261 aattttcttt tttcctctca tttaccgggg ggacctggga aatatcattt gattgggttt
     1321 tttaaaaatg taagaaatgc attttacgac aacaacagga gtcggatgca taaaaaacct
     1381 catccatttc tgctcgtcct gaaacgcgcc gtaaaaattt aactgtttat cagttggtat
     1441 ttacacaatt aatctgttaa ttgagtatta actgattaac ttactagtaa tcagtgagaa
     1501 atcagaaaac gatcactact cgaataaaat ccggtaaaaa cgaattaatt cgtgaataga
     1561 aggtgtaaaa agagatctta atttgttaag aataatttaa tagtattgaa attttcttat
     1621 tgtaaacaaa aatcctttta aaaccttcca acgaaatttt cctttgtctc taatcggttt
     1681 taaaaattca cccataatga ggtgcactgt gaaggaaatg aagaatgtat gggcaatcct
     1741 gctcattgtg attgcagcac tggtgcttac ggcaccggtt atcgcagcaa gtagtacctc
     1801 gcttaccatc accaagctgg caagcgatgg tacgaccgtg cttgacacac gaacggtcga
     1861 ttatacgtgg atgatgacca acctccctgt gttaggagat gggactaccc attactatgc
     1921 ccagggaccg gtgttcatag acgatcccga tccggttatc gagcagcagc ttcggtggaa
     1981 tcctgatgag gataacaaca tcgataagga catgggggct gtcaaaggta cgaatctcaa
     2041 ggatctctgt gatctcgtcg gaggaatgaa tgccggtgaa accatacagg tcacggcaaa
     2101 cgacggcttt accaagtact ttgcttacaa gaacatctac gaatactcca ctcgcgaagg
     2161 tccgatggtg attgcgtggt atcaaaacgg taactatccc gacactggtt atagtgacgg
     2221 tatgcgtctg gtctggatgg ccgatgattt agtaaatccc aatggtaacc acgttttcgg
     2281 aaattatgac tggtaccttg cggccgatga agcttactgg tactattacg taagtggttc
     2341 cgagaagtat ccgactacat cgggactctc ggtgactttt gtcagcgata taaccatcta
     2401 cagcgatgat cccgcccctt cgatggacgt gctctttgat ggtacggtag tcctcacccc
     2461 aggagagacc tttgacaagg tagcctacaa cacaggtaca acctataccg tcagccggac
     2521 caccccgatg ggtgcattgg atgttgcagc gaccacagcc gggttcacct acgatgtgac
     2581 cgacaagaac tatgcgactt ccggtgcact gctcctggac aacgtgggca cctacctccg
     2641 tgacaaaacg aacggtatct actggtatgc ctacgtgaac gatgtctaca aggatgggta
     2701 caacaaccct gccggcggtc tgaacctgat agagctgaat gatggtgaca aggtcgagtt
     2761 ctactacgcg gccggcattg cagatccagc cgatctcgct gccgtaaagg cagcagccac
     2821 tgctgcggtc aagattgttg ccgacaccac cccgggcccg gtcatggacg tgctctttga
     2881 tggtacggta gtcctcaccc caggagagac ctttgacaag gtagcctaca acacaggtac
     2941 aacctatacc gtcagccgga ccaccccgat gggtgcattg gatgttgcag cgaccacagc
     3001 cgggttcacc tacgatgtga ccgacaagaa ctatgcgact tccggtgcac tgctcctgga
     3061 caacgtgggc acctacctcc gtgacaagac aaacggtatc tactggtatg cctacgtcaa
     3121 cgatgtctac aaggacgggt acaataaccc tgccggcggg atgaacctga tcgaactcaa
     3181 tgatggagat aaggtcgagt tctattacgc agctggtatc accgatcctg cagacctggc
     3241 agcagtgaaa gcaacagcta ccgccgcggt gaagaccgtt gcttctaccg gtgtcgcgcc
     3301 aacggactgg tccatcgtca tcgacggtgc gaagacccag accgtgacga agagctactt
     3361 tgagaacggt cttgcctgtg cagcttccag tcaccaggtc ttctggaccg atgatgatga
     3421 caatgtgtgg ggcggtgttc cgctctggct ccttgtcgcg atggttgacg atgatcccga
     3481 tgtcggaccg gatcacttca acttgaacga tgatctcgca gcacagcatt acgaagtgaa
     3541 ggtgatcagc tccgatggat ggtcagcggt cttcgacagt tctgctgtcg cccggagcag
     3601 cgactacctc gttgccaata ccctgaatgg cgaaccgctc atctacggtg gggagaagcc
     3661 ttcctggccg ctccacctga agggcgcagc ggttctcggt ggccagcagg taggcggcat
     3721 tgtccgcatc gagctgaaca acctcccgga gccacccgcc ggctggacgc ttgagatgct
     3781 cggtgaagtg ggcgatgtta tcacccagga agagtttgaa gaaggcctcg cctgcccagt
     3841 cgccgatcac tacgtcgaat ggatggatgc atcgggcaat gtctggtcgg gtatgccact
     3901 ctggctggtc gtaggtgcgg tggatgacat cgagcttacc gacagctata ccttcaatga
     3961 tgaggtggca gccgccggat ataccgtcaa aataacggca atagacgggt tcaacaagac
     4021 ctttgacagt gcggttatta cccgcaacaa cgattacctc gtcgccaaca agataaatgg
     4081 cgaaccgctc gatgagagca tgttcccgct ccgcatggtc ggggcagggg tcacaaaacc
     4141 tgatggctca ctcggtggat cagcagtggg gaagattgca aagatcgaga tccttgaact
     4201 gcagacaccc ccggctgcac cgggcagtta caaccttgcc gtcaaaggaa agatcaccga
     4261 tgtcttctca caggctgagg tcgaagaggg actcgcctgc ccgtcgtcag gacacttcgt
     4321 gacctggaca caggagatca aggatgacag tggaaccatc atcgagaccc atgaatggtc
     4381 cggcatcccg ctctggttcc tggccggctg ggtcgatgac cgggtcccgc acgagttcaa
     4441 cgcggtccag gcctctgccg ggtacaagat aaccgtaaga gcttctgacg ggtatgccaa
     4501 ggacttcccg tctgctgatg tggcctggag caatgattat atcgttgcca ccaccaagga
     4561 tggcgccccg ctgcccgatg gcaagtggcc gctccagctg gtcggtgacg cactggccaa
     4621 ggcggacggg accctcggtg ggatgagtgt ctcgaagatc gccgagatcg agcttactga
     4681 atttggagta ccgaccgaaa tcccgaaggt ccatatcgtc aagtacgctg ctgatggaat
     4741 gacggtcgtg aacgaggtta ccctcgacta cacccagatg caggagcagt tcatggtcgt
     4801 cggtgatggg aagacccaat accggtacca gggtgtcacc atggacccgg aggatatctg
     4861 gggagagacc gatgagacca agggcggata caagatctcc aatgccgtga aaggtacccg
     4921 ggtggctgac ctggtgagcc ttgtcggcgg catgggcgaa ggcacggata tcgtgttcgt
     4981 tgccagtgac ggtttcaaga ccacactgcc gtacacgtgt attttcacca cccctgatat
     5041 tcaggcacgg cagggtgatg cgattctcgc ctggtatgcc gacgggaaat atgtacccgg
     5101 ttatgcagac ggtatgaggc tctttttcat gcctgaggat accatctacg gccaatggga
     5161 catgcatgag accatcccgg caggttactg gcactactac taccaggcat ataacgccgc
     5221 tgacccgatc tatggtaaat atgcaccggg tatcctgtat ccctcctgtg cagggctttc
     5281 ggcgaagtac ataaccgaga tcaaggtcta tacgacacca gagtcgacct gggacctcca
     5341 ccttgatgga acaagggttg ggggaattga gcatacgatc gcaaagggct atttcgaagc
     5401 agcacttgcc tgccaaatgg gggcaaacca cgatgcgagt tacactgatt ccagcgggaa
     5461 cgtctggacc ggcatgccgc tctggttcct ccagggattc gtggacgatg ccgaccagca
     5521 ctccaacaat gcctacaacg aggcacttgc attggagggc tacgatatca tcgtgatggc
     5581 atccgatggc tacaacaaga ccttcagtag cgttgacacg gtccgcagca cgggatacat
     5641 cgtggctaac acgctcaatg gataccatat ccctgagtct gacgccagtt ggccccttcg
     5701 gcttgtcgga gacgacgtac ccaagaagga caatgtcaag aaggtcagtg atatcatcct
     5761 gaacttcaag ccaaatgtgt ccatggcgta tccgagccag gaatcgctct ggcctttgaa
     5821 caagaattac gtgaatgtca cgatcgaagg agttgccgat ccctacgatg aagtcactat
     5881 ctccatcata tcgatcacca gcgatgagcc aaccacagcc ggaggaaaga accaggcccc
     5941 cgatgccgat ccatcctgca ttggcacaga caccgccagg attcgtgctg agaggatagg
     6001 gaatggaaat ggacgggtat acgagatctc cttcgtggcg accgacagca tgggcgcatc
     6061 aatggaaggc accgtgaacg tctttgtccc gcacgaccag ggcatggtct ccgagtgcat
     6121 cgatgacggc cagatatacg acgccacatt gataatctga ccgatctcgc aagagaacaa
     6181 tggccatctg aaaaagatgg tataactctc ttttttttaa tcctttgaaa caggcttatg
     6241 gtgcattata gaatcctaaa aactggtccg ataaaaacga tcaggtggtg cattgtgtcg
     6301 atgaatgttg gggatcagga caaaccgctc gcggatcgca tggatggcac cctgttcgaa
     6361 aaatggaaac gcggagaagt gggcatcatg atccttcatc ccctccctct gatgtcccgc
     6421 caccctcccc gaccccgtct ccatcatctc ccccggtcgg cacgctgatc ccgcaatggt
     6481 actcgtcttc cgtgcaccag acctcgatct cgggcgattc gggggttccc ccaagcggtc
     6541 gttcccgccc gggaaccacc tgctcttcac ggaccacctg cccgttcttc atccggtaga
     6601 caagccggaa ctcccacgta tccccaaggg tgaccgcccg gttgaaatag acctcccggg
     6661 caagaccata agagcagtag agggcggggg agatctcgaa caggactccc cgcatgtagc
     6721 gtttgacgta ccaccggagg tcagcaacaa gggagagtgc actaccaagt gaggcaaccg
     6781 tgatggcaac ccctgaaggg gtctccaccg ggcggtagaa ccggagtgcc tcccggctcg
     6841 tctcggaggt tgagagttca tggtacaggg cgacgccatc cttgggaagg aagaggacgt
     6901 tcatggatct gttgcagata ctttggtcac ccggaattta taggctcccc ggttggatcc
     6961 ctttccgggc aacatgggca ggccagagcg gagtatcgtc cagtgacggg gatccggcat
     7021 ttcttatcag agaatacctt cccggagacg caaaaatgaa cgcccgggtg caatacccgt
     7081 tgagcccagc ttccttcttc acgtcacctg ccgaaaagcc ggaggggctg aggggagttc
     7141 ccctgtcggc atttacctga accggttcca ggagattatt ccccgttgct ccgggcttgt
     7201 tcccggatca gtgcaaaagc ctcttcaggt gaatcgattc gattcacgac accatctttc
     7261 atgaccgaga aggatgtgcc gatctggcct gcttcccacg ggtcgtgggt gatgtagagg
     7321 actggaatat gcaactgcag gaggacatcg cggaacagcg gaaggatata ctccttcttg
     7381 gcccggtcta cctcggtgat aggctcatca aggagcataa cccgcggtgc tgataccagc
     7441 gccctggcaa gagctgcccg cgcccggtag ccccctgaga gtttgtgggg tttccagtcg
     7501 agatagggtt caagagagag caacttcaca attttgtgga atgtctcttc tgatcccctg
     7561 ccgttctgga ggggataaag aatattctcc ctgacggaga ggtgcttgaa cagggctgta
     7621 tgctggggga cgtacccgat cctgcggtcc tcgggcggga ggccggtaat atcgacactt
     7681 cctatactga ccgttccctg gtcagggtcc aggatgccgg tgaggatccg ctggagcgtc
     7741 gtctttccgc atccattcag ccctatcagg acctggattt cacccggatt aacggagatc
     7801 gacactcctt taagcacctg gtgggcagag aatgatttat gcagattctg cgcttcgagc
     7861 atgcaacctc cccctcaaac gaaggacaat caccagcatg agggaaagca ggatacagaa
     7921 cgcgctggct gttaccgcca tgctgacttc cccttcttcg accttggtaa agacgtaact
     7981 ggcgagcacc tgggtcattc ccggcacatt gccgccgaac atgataacgg ctgcaaactc
     8041 cgatatcgac cgtgaaaagc tgagtacgaa tcccgagaag attgcttcac cgagcaacgg
     8101 gataaccacc cggcgatacg ctttgggagg agtatcgccg agcgtccgtg ccacgctgat
     8161 ctgttccttg tcgatctcct ctgctgcaga ggcgatagtc cgtgccgcca gtgggtagga
     8221 gacaaagaac atggcaagga caactgcaat gagcgtgaat gcaaggccgg ttggtccgat
     8281 gaacccgaac atgttcctcc cgaatgccag cagcagcgca agaccggcaa ccgtatgggg
     8341 tagggcaatg ggaaggtcgt tcatgaaatc agcaatcttg tagatgatcg tcccggggtg
     8401 gaagatgtag aagtacccga acccaagcgc catcagtacc ccgagcagag cggcaaagaa
     8461 ggcggtgatg agggtgttct tgaccgaatc ccagaaaatc gggtcatcaa aagtctttat
     8521 catcccttcg atggtcacat tcccgatgat ggcgaggatc gggtagacga tcagcaggaa
     8581 aaaaaggggg acaaagagga cctcccagat actcacctta cgcatacagc tcccagccat
     8641 actgggtgaa ctccacgctg ttctctcgga caaaatccct gaaggcctct gctcctgcct
     8701 cgtttccacc cttgattatc gcatcgccaa agactatctc ctccgagaga tcgtagccat
     8761 acttctctgt ggtaacgaga tcgtaaagaa ccgctgtact gctgtaggtg aatccggcat
     8821 ccacctcatc actctgcacc atcagggcta cctggttcac ggtgcttggc cgggtcttga
     8881 tattgcccaa tgcgttcagc tggtcccaga ggccaaggtt cttcaacacg ttctcggaat
     8941 agaccccgac cggagcactt ggatcggcaa ccgccaccac gatgttctca ctagtgaggt
     9001 tggccgctgt cgtgatccct ttctcctggc cagtctttga aaccgtcatc acgacaaact
     9061 ccttcatgaa cttttctgcc tcggatactt tgccctccgc ttcaagtttc tcgatcaggt
     9121 tgtaatccgc agaatagtac aggtcacagg gctgtccctg gctgatctgg gtataaagtc
     9181 cgccggaagg cccatagttg gggatgacgg taatcccggt ggcattggtg tatttcgcaa
     9241 ttaccacgtc catcggtttc ttcagacccg ccgcaacgta cacattcagc ggggcttctt
     9301 ctgtttgggt acaacctgct gacaggagaa ggaatccaac aagcaaaaac aatccgatta
     9361 ttcctataaa acgatgtggt ctttccatgg tatcaccgtg caatcatacc agcaggaggg
     9421 taatgtcctg cggatacctg gaaatttttg cgactttgag aaataaatca tatcatggta
     9481 acattaaata tcaagtcaac aatataaact tatcaattga tatatcaagg cattacaaca
     9541 ctaggatacc cggttccaca aattcaccca ccacgcttct ccttcgctcc ttccttgtgc
     9601 caaggatcgt cttctccgga ggccttactc ctcctgcaac ggtactcttc ctctgtgcac
     9661 aagacttctc gtccagattg ccctgaatac gtcagttcca ggccggcaag gatctgctca
     9721 tcccggacta cgaatccatc ctgcgcctgg cagatgagga ggaaatccaa cagaactgac
     9781 cggacaattt ccctgctgta atacatgtcc cggggggtta tatgagcggt agatccttgg
     9841 tacgatctta aaaaatgatc ccgtaccaag cactccagac accagcggag tttaactgaa
     9901 gaggcgcgtg ctcagctgag cgttgctacc ttatcgcaat ctcattcggg atcctgggat
     9961 cgtagaacag gagggactct cagatcgtct cgaaagcagg gagtccgcaa acaagcaaca
    10021 caccttcacg tggcatggag aggatcgttg tacagcatgg caccctggaa gagaaccgtg
    10081 ggcggtgggg ttaaaatctg ttttgtcagg cagagaaaat gaaggggagg ggagatcaag
    10141 tatacatccg gagatcagtg acgacctccg ctttcacttt ccggaccgct ttctcaaagt
    10201 cctcctcagt gaccccgtcc gcctccctcc ggacagccat catgccggcc tcacggcaga
    10261 ccgcctggag atcagccccc gtcgcgtttt cggtcatgtc gacaatccgg tcgagatcca
    10321 catcgatcag agtcatcttc cttgagtgta tcccgaggat tgccttccgg ccggccttgt
    10381 ccgggagcgg gattgcaatg acccggtcga aacggccggg gcggaggagg gccggatcca
    10441 gcatgtcgac acggttggtg gcagccatga tccggacatc gccccggtta tcgaacccgt
    10501 ccatctccgc gaggagctgc atcagggtcc gctggacctc ggcacttccg gaagtgccat
    10561 cgttggtgcg ggtgctcccg actgcatcga tctcgtcaat gaagacgatc gtgggtgcct
    10621 tttccctggc aagcgagaag agctcgcgga ccagctgcgc cccttcgccg atgtacttgt
    10681 ggacgagctc gctccctgac atccggatga atgtcgcctt cgcttcgtgg gcaaccgcct
    10741 ttgctatcag ggtctttcct gttcccggtg gaccatagag caggatcccc ttcgggggtt
    10801 ccaccccgat ccgtgcgaag atctcgggct tggtgagcgg atactccacc gcttcccgca
    10861 cctcctcgat ctccttctcg agtcccccaa catgatcaaa ggtcatgtcc ggcgattcct
    10921 caagctccat cacccggacc cgggagtcaa aaatgttccc gacaatacgg acgatagaga
    10981 gtgcgttgtt gaccgcgacc ttggtccccg gcttgagctg gctgaagagc tcatcgttca
    11041 catgcgtgat gtattcctgg ttattcccct gctgcctgag gtatacctcg ccttttccca
    11101 aaagatcgat cactacggcg acaaaaaggg gcatcctctt gagctgatta ttctcccgct
    11161 tgagctgggc attgtcccgg gccagctcat cgttcctcac cttgaggtca aggagctggg
    11221 actcgaggtc ctgtgccttc agctgccagc tgagttcaga ctcacccgat ggggtgagga
    11281 tggtttcttc cattatacta ttatattcgg attcctacca tttattaggt gtgattttga
    11341 tagtccatgg gcgagggata tcacggggga gagggacagg cgagctcctt gtcagtcctg
    11401 ctgccatctc cttcctctcc ggagttgatc ctgacacggg tatca
//
LOCUS       DAOOCO010000044        11427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5309_length_11427_cov_5.186599, whole genome shotgun sequence.
ACCESSION   DAOOCO010000044 DAOOCO010000000
VERSION     DAOOCO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5309_length_11427_cov_5.186599"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK154_03870"
     CDS             complement(<1..676)
                     /locus_tag="PK154_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10233.1"
                     /translation="MTSAVHDRMNHTSYAGRMQPVPTWITGEGIIRLISLEGEGFFGI
                     VTGAGDQYLPDNLPRSLAVDGIRVTFRGIATESSQGVLMWGTRLHLISIDKTGDTFTS
                     SGTVTFVDLEGGFFGIVTDAEDHYLPLNLPEEYRIDGMRVVFTAYEERDAATCAMWGI
                     PVQLASIAPVDQPFGTITGFWYLNSYSDGESLRPVLPGTSISAEFGDDGTITGTSGCN
                     HYFGSYK"
     gene            1166..1606
                     /locus_tag="PK154_03875"
     CDS             1166..1606
                     /locus_tag="PK154_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPW10234.1"
                     /translation="MSDLSDFEATLKHIIELGGEETATKWMENIEAEYGRAPLIFKRM
                     AERPEVLISHLLYKSTVTGTSALEPKYVELISMAVGAALKCQHCTSYHLQAAAKKGAT
                     REEILEVILIAGLISNSSVLANAYRIFDEKMAKCIPCESTGVAP"
     gene            complement(1627..3471)
                     /locus_tag="PK154_03880"
     CDS             complement(1627..3471)
                     /locus_tag="PK154_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPW10235.1"
                     /translation="MIRCKTDQPDFIPLSMGEAGRLGIDTFDIIFVSGDAYVDHPSFA
                     TALLSRALWEKGFSVGIIAQPDWQQPRDFLKLGTPRLFFAISAGNIDSMVSHYTARKR
                     LRSTDAYSPGGRRRRPDRPSLVYADRLHSLFPETPLVIGGIEASLRRFAHYDYWSDRV
                     RQSLLADAPADLLVFGMGELPLFMIADRLSSGEDIHEITTIPGTVIKKGIHSPPDDSF
                     HDPVIVPSFSDVSSDKRAYARAFGMIHAEQDPYRGRRVIQAHPKVQIIQNPPSRPLST
                     DELDWLYELPFSRSAHPHYREPIPALEPVRFSIVSHRGCFGNCSFCAISHHQGCIIQS
                     RSVSSIAREVRRLTTHPEFRGIVQDVGGPTANMYGMFCNHWESRGTCPDRVCRPDCPN
                     LATSHEPQVTLLETLRQIQGVKRVFVSSGIRYDLIGTDEAGYLGALCTWHVSGHLKVA
                     PEHISPHVTKLMGKPGREDFDSFRQRFEEQQQGKKKKQYLVPYFMSGHPGCTIADMIE
                     LAEYIRDNHLYTEQVQDFTPTPMTVSTCMYYTGLDPCTLEPVHVPKDREKRIQRALLQ
                     YRDKANHELVREGLSVAGRADLMGDGPDRLIPGLPGHHRKRNKRGKMA"
     gene            complement(3468..3917)
                     /locus_tag="PK154_03885"
     CDS             complement(3468..3917)
                     /locus_tag="PK154_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPW10236.1"
                     /translation="MQYTEGELGRVFQVRIDDGEDLLDTLQVFVREKEIRCGFAQFIG
                     ALKGGRIVTGPKELVLPPDPSFETYQNGWEVFGYASITPGPERAHLHYHASVGKNREV
                     MTGCLREQATVYIIVEVLIIEILGSTVSRKRDRVTGMELPFFGDPGK"
     gene            complement(4057..5874)
                     /locus_tag="PK154_03890"
     CDS             complement(4057..5874)
                     /locus_tag="PK154_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPW10237.1"
                     /translation="MDEKRQIAILIAVAFGIVVGACILALAVPMVTEGDLVIADYQAT
                     LYRDGTFNETYLYEVEAPGQYRMLYRYWDDPLALGPLNVPFIELKGMEVPPPAIGYVK
                     EYDGMVSIYNGDFEGYTEFIGYLAERNEVGIFEPSYFSTGIYEVNYSYLLHPPLEYDT
                     SSAHLNLKLVGDQHISFREVTIRVPSDLVESVYPHPPSLTVREDGAWIVISGSVPADE
                     VIGVEMLLSMDALESIPGFPVFTEDVVERTADANPWYAILPILLAKLLLILGFAGAII
                     TPFFLLYVYYRYGREQEFTVPEYLSTIPNPTMKPWEVNLLFKGDATVFDEDGYYATLI
                     DMHRKKMITIIEDGWGNVRVSLHAKESEDPYEQSVLTFLGEIASDGIVNSANLEILAS
                     KAKSDQAAERKMLKYQKNLAALVRRSDPSLISHYVVDGREHIFPALFAGIVFCVLSFI
                     LILIAGILAYLIIPAIILFGSVIVQSGVALAFPSTLFGYWKGDSYKEKLEWDAFAKFL
                     SDLAMLRKYAPADISMWGTWLVYGTALGVGDRVEKAMKELNISIPETIAPATGARSAF
                     LPVLYFSPPSRGGGGSSGGFGGGSFGGGGGFGGGGVGGR"
     gene            complement(5891..6463)
                     /locus_tag="PK154_03895"
     CDS             complement(5891..6463)
                     /locus_tag="PK154_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPW10238.1"
                     /translation="MAWESLTPWIIGGLILVLLIGFVLYFIGIYNRFFSLKNSSEATL
                     GQIRVAMKKRLDMIEQLLGSVKSYAQFEREVFEKVTAMRARVGSAGPGDLNEIERESR
                     SVLGRLFAVAENYPDLKTSTTVMNLMDAVREVEEEIARQRYTYNNISQQYNTLVDVIP
                     SNFIARILGLGKLAYLEFEEAIATPPKIEF"
     gene            complement(6528..7490)
                     /locus_tag="PK154_03900"
     CDS             complement(6528..7490)
                     /locus_tag="PK154_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPW10239.1"
                     /translation="MSNLTVAVIGPEGYAQDLGKKGTSSDITFYNLKKGDNTITFLEP
                     ARYPERLASLFFSVSMAARAILVVDEINAAFGECLIMLDCAGIRKGILVLRNYITPEQ
                     IAPLIQGTVVEHYRLLPDDRMRIREELIAEIEAVKEPDQIRDHGAVPVDHFFNVKGIG
                     VVVLGEVIGGRICRHETLRVHPTKKTALVRSIQKHDDDAEYAIPGDRVGLALKGIETE
                     DLDRGYVLSASTDITSSSLINGRANLVRYWPSPLKEGMVLHAGLWMQFIPARIAFVDN
                     SGDWKKPVITLRTESELVYRPGARVILHYLEGGKLRVAGTIVLE"
     gene            complement(7519..8382)
                     /locus_tag="PK154_03905"
     CDS             complement(7519..8382)
                     /locus_tag="PK154_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HPW10240.1"
                     /translation="MYVMVSPCILHPQLRAKGITRDKDLEWFSRAIQRCYQYGIEVVP
                     LPCPETLCLGYDREPGVFLDRLDTKEFADLLDLLEEKVREIISKRGPPLCIVGVNSSP
                     ACGVNTTWYGPRGSPDARRRGRGAFLSRFPDLPAIDVSDFSRYRVYLAAPLFSLAERR
                     YNIQLADLLRRNCYEVYLPQDSGDDSHKRERDAHKAIFGQNLAALDSSDVVVAVIDGP
                     DADSGTAWEIGYAFSKGIPVISLRTDFRKAGYHEDVNLMLEHSSTVVHSEEGLVAALL
                     FPGGFQAAHKC"
     gene            8413..8904
                     /locus_tag="PK154_03910"
     CDS             8413..8904
                     /locus_tag="PK154_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HPW10241.1"
                     /translation="MIPLYAFRDNSCDPKKCTVKRLERSGLVRVLSKISHIPRNTILL
                     DPTAPQALSPADRPARSLTALDCSWEVLDTGAVSSWRKRRALPFLVAANPVNFGRPYR
                     LTSVEAFGAALFILGEREQARGVLESQNWGPRFLELNEEPLSLYARAGDSTEIIAIQK
                     MFL"
     gene            complement(9157..>11427)
                     /locus_tag="PK154_03915"
     CDS             complement(9157..>11427)
                     /locus_tag="PK154_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW10242.1"
                     /translation="ENFLSFFGLKKEDTIGSPIGSCPVNSLGSAKIEDLARDAFDSGE
                     IITETSYARDGQTCYLRIKAVPSVFDDATRGSTLIFEDITEKKQFEDRLKLSEAKFRA
                     IVEDQTELVGRFDKDLRILFVNKAALQYYGITNEDFNGKKFLEFIQPDDRARVITCIE
                     GLSFEKPVDTVENRVLFPDGTQRWLQWTNRAIFNTEGTLIEYQGVGRDITERKKAEEA
                     LVVKDLALSTSVNGIALADLNGVVTYANKAYQRLFGYQNDAEVIGRPIQAFSHGDPQE
                     AAIITTVIDSLMMKGSWSGEVTPRRRDGTPFYAFLSAWMVKDTLGIPFCMMASFADVT
                     DLKKAQEEIQLKNTAIATTINPIAIFDANQRLIYANDSFIEEFGIPMNEIRGRQPRDI
                     FAQFETVSPSLEEIERALSDKGRWKGEMVVRKKDGSMMYLEGSIRITVNGEGRLLYML
                     ASFVDITGHKEAETALRSSRQKLEETIEFMPDPTFIIDRNHRIIAWNRALELLSGVKR
                     EEVLGREDFQKAFSFLKGSRPVLVNLLDLPPHEIARNYPSVRRFGDSIYVEAYIPDMN
                     GGKGSFLWGKASALVDNEGHRIGAIESIRDISAWKRARESLKNAETRDPIGTMPFLGE
                     ENKMQEGSIARDELEAILNLVDEAILVLDSSGIVRWANENFIKIVEGEREFVLGAHVS
                     IFFPGFEQQILGEYDAKPENNNLIVHLMPLTTHRVLPVKVRTVGLQGTDGKILILQQY
                     TPLSYASTGEGSMDVP"
BASE COUNT         2762 a         3056 c         2840 g         2769 t
ORIGIN      
        1 gcttatacga tccaaagtaa tgattacacc ctgaagttcc tgttatcgtc ccatcatcac
       61 cgaattcagc tgagatgctt gttcccggta aaaccggccg gagggattct ccatcgctat
      121 acgagttaag gtaccagaag ccggttatcg ttccgaaggg ctggtcaaca ggggcaatag
      181 atgcaagctg cacaggaatc ccccacattg cacaggtggc ggcatcccgc tcttcgtacg
      241 cggtaaatac tacccgcatg ccatcaatcc gatactcttc agggaggttc aggggaaggt
      301 agtgatcctc tgcatcggtt actatcccga aaaatccccc ctcaagatcg acaaaggtga
      361 ccgttcccga tgaagtaaac gtgtccccag tcttatcgat gctgatcagg tggagccggg
      421 tcccccacat caataccccc tgggatgatt cagtggcaat acctctgaag gtaacacgaa
      481 tcccgtcaac tgccagagac cgggggaggt tatccggcag gtactgatca cctgctcccg
      541 tgacaatccc aaagaatccc tctccttcca ggctgatgag tcggatgata ccttcccccg
      601 ttatccaagt gggtaccggt tgcatcctgc ccgcataact ggtgtggttc atacggtcat
      661 gcacagcaga ggtcatgttg aatctccgat ctggcaggtg agatctccag agagttccgc
      721 ggtcaggatc tgagggcatc cttgcatttt caccatcgga gtctattgag cgaagggctg
      781 gcttctgcat agaaaatccg tttggtactg attctgagct tgggaacgct ccgcaggttc
      841 ctgtattccc tggtaaaggc tgcttatacc agattcctga tactggcgca ataccaccat
      901 tctgcctgtt ctccaaggtg ggagcagcag ttcctggtga aaaaatcgat gccaggacca
      961 cccccatcag cattaccgac aagaatctga agaaatattt cctgtctgtc atatgttcac
     1021 ctcattattc tccattgctg gattatctgg atactggaac atattttgtt aaatacttcc
     1081 tctaaagtgc gaatagtttc gcagtatccg gccggctccc gcttccccat tcttaatgtc
     1141 ggacagaagc gataggatct atcacatgtc tgacctgtct gactttgagg ctaccctgaa
     1201 acatatcata gagctgggtg gagaagagac cgctacgaaa tggatggaga acatcgaggc
     1261 tgagtatggt cgtgccccac tcatcttcaa gagaatggct gagcgcccag aggtcctcat
     1321 ctcgcacctg ctctataaaa gtacggtgac aggaaccagt gctctggagc caaagtacgt
     1381 ggagctgatc agtatggcag tcggagctgc cctcaagtgc cagcattgca ccagttacca
     1441 cttacaggct gccgcgaaga aaggagcaac gagagaggag attctcgagg ttatcctcat
     1501 tgccgggctt atctccaatt catccgtcct tgcaaatgcc taccggattt ttgatgagaa
     1561 gatggcaaaa tgcatcccct gcgagagtac cggggtagcg ccataatatc ggtatctgtc
     1621 atcggattat gccatttttc ccctcttatt cctctttcga tgatgccctg gtaaacctgg
     1681 aataagacgg tctggtccat cgcccatgag atcagctctc cctgccacag aaagaccctc
     1741 ccggaccagt tcatggttgg ccttatctcg atactggagc agagcccgct ggatacgttt
     1801 ctcccggtcc tttggcacat gcaccggctc aagggtgcag gggtcaaggc cggtataata
     1861 catacaggtg gaaacggtca taggagtggg agtaaaatcc tgcacctgtt cagtgtaaag
     1921 gtgattgtca cggatgtact cggcaagttc tatcatgtcc gcaatggtgc agcccggatg
     1981 ccctgacatg aaatagggga ccaggtactg ctttttcttc tttccctgct gttgctcttc
     2041 gaacctctgc cggaatgaat cgaaatcctc ccggccgggt tttcccatca gttttgtaac
     2101 atgcggggag atatgctcag gcgcaacctt gagatggcct gagacatgcc aggtgcagag
     2161 cgctccgaga taccctgctt catcggttcc aatcagatca taccgtatcc cggagctgac
     2221 aaagactcgt ttcaccccct gaatctgccg cagagtctcc agcagggtga cctggggttc
     2281 atgagaggtg gcaaggttcg gacagtcagg acggcagacg cgatccgggc aggtaccccg
     2341 gctttcccaa tggttgcaga acatcccgta catgtttgct gtcggcccgc caacatcctg
     2401 gacaattccc ctgaactccg gatgtgttgt caatctcctg acttcccggg ctatcgatga
     2461 gacactcctg ctctggatga tacatccctg gtggtgggag atcgcacaga acgaacagtt
     2521 cccaaaacag ccccggtgac tcacgatgga aaacctgacc ggttcgaggg cagggatggg
     2581 ttctcggtaa tgaggatgag cgctcctgga aaaggggagt tcgtacagcc agtcgagttc
     2641 atcggtcgac aaggggcgcg acggaggatt ctgaatgatt tgcactttcg gatgtgcctg
     2701 gattaccctt cttccccgat atgggtcctg ttcggcatga atcatgccaa atgcgcgggc
     2761 gtacgcacgc ttatctgaag agacatcgct gaacgaagga acaatgaccg gatcatgaaa
     2821 agaatcgtcc gggggggaat gaattccctt ttttataacg gtcccgggaa tggttgtaat
     2881 ttcatgaata tcttctccgg atgagagccg gtctgcaatc ataaaaagag ggagctcgcc
     2941 catgccaaag accaggagat ctgcgggtgc atcggctaga agggattgtc gaacacggtc
     3001 ggaccagtaa tcgtaatgtg caaaacgcct cagactggct tctattcccc cgatgaccag
     3061 gggggtctcc ggaaagaggc tgtgaagccg gtctgcatac acgagtgaag gccggtcagg
     3121 acggcggcgc ctccctcccg gggagtatgc atcggtggaa cgaaggcgtt tccgtgcggt
     3181 gtagtggctc accatcgagt cgatgttccc ggcagagatg gcaaagaaaa ggcgtggtgt
     3241 tccaagcttg agaaagtcac gaggctgctg ccagtccggc tgggcgatga tcccgaccga
     3301 gaagcccttt tcccagaggg cacgcgagag aagggcggtc gcaaacgagg ggtggtcaac
     3361 gtatgcatcc cccgatacaa aaatgatatc aaaggtgtcg ataccaagcc tccctgcctc
     3421 gcccatcgac agggggatga agtccggctg atcagtcttg caacgaatca cttgccagga
     3481 tctccgaaaa agggaagttc catccctgtc accctatccc tcttccggct gactgtactt
     3541 cccagtatct caatgatcaa cacctcaaca atgatataca ccgtagcctg ttcccggagg
     3601 cacccggtca ttacctcccg gttctttccg accgatgcat ggtaatgaag atgggctcgc
     3661 tccggtcctg gtgttatcga ggcgtaccca aacacctccc agccattctg ataggtctcg
     3721 aatgaaggat cgggggggag gaccagttct tttggcccgg tgacaattct ccctcctttt
     3781 aaagcaccga tgaactgggc aaaaccgcac cggatctcct tctccctgac aaacacctgg
     3841 agggtgtcaa gaagatcctc cccatcgtct attcggacct gaaacaccct gcccagctcc
     3901 ccttcagtat actgcatgat agctccccat aaggtacctg tacagtgccc tgccggaata
     3961 aacatgtcat tcatggaggt cgggtaccag tatgctctgc caacggatat acccaaatcc
     4021 gccaacagaa tagcggggca aagggaaccg cacctgttac ctccccccga cgccaccacc
     4081 cccaaagcct cctccgccgc caaaactgcc gccgccaaaa ccaccggaac tccccccacc
     4141 acctcttgag gggggtgaaa agtaaaggac tgggagaaat gcactccttg ccccggtagc
     4201 tggcgctatc gtttctggga tcgaaatatt cagctctttc attgctttct cgacacggtc
     4261 cccgacacca agagccgttc catataccag ccatgtcccc cacatagaga tatcagcagg
     4321 agcgtatttt ctgagcattg cgagatccga taaaaatttt gcaaacgcat cccattcgag
     4381 cttttccttg taggaatcac ccttccagta cccgaaaagg gtcgatggaa atgccagtgc
     4441 caccccggac tggacaatga ccgacccaaa aaggatgatc gcagggatga tcaggtaggc
     4501 aaggattcct gcaataagga tcagtataaa tgacaggaca cagaagacga tccctgcaaa
     4561 gagggcgggg aagatgtgct ctctcccgtc cacgacataa tgtgagatca aggatggatc
     4621 cgatcgcctt acgagagctg ccagattctt ctggtatttc agcatctttc gttcggctgc
     4681 ctggtcgctc tttgccttcg atgccaggat ctcgagattc gccgagttca cgatgccatc
     4741 cgatgcgatc tccccaagaa acgtcaggac gctctgttcg taaggatctt ctgactcttt
     4801 tgcgtgtaag gatacccgga cgttccccca gccgtcctct attatcgtga tcattttttt
     4861 cctgtgcata tcgatgaggg tcgcataata gccatcctcg tcgaaaaccg tggcatcgcc
     4921 tttgaagagg agattcactt cccatggctt catggtggga ttggggatgg tactcaggta
     4981 ttccgggacg gtgaactcct gctccctccc gtatcgatag tatacataaa ggaggaagaa
     5041 gggtgtgatg attgctcccg caaatccgag aatgaggagg agttttgcta agaggatggg
     5101 aaggatggca taccagggat tggcatccgc tgttctctct acaacatcct cggtgaacac
     5161 agggaatccc gggatcgatt cgagcgcgtc catcgaaagg agcatctcga ctcctatcac
     5221 ttcatccgca ggaacgcttc cggatataac gatccacgct ccatcctccc tgaccgtgag
     5281 agagggggga tgggggtata cgctctcgac caggtcagac ggaacccgga tggtgacctc
     5341 acggaaggag atgtgctgat cgccaaccag tttgaggttg agatgggcag atgacgtatc
     5401 atattccagg ggcgggtgaa gcaagtagct gtagttcacc tcatagatgc ccgtagagaa
     5461 ataggaaggt tcgaagatgc ccacttcgtt tctttcggca aggtatccga taaattcggt
     5521 atacccctcg aaatccccgt tgtaaatact gaccatgccg tcgtattctt tgacgtatcc
     5581 gattgctgga ggcgggacct ccatcccttt cagctcaatg aaaggaacat tgagtggtcc
     5641 gagagcgagc ggatcatccc agtaacggta gagcatccgg tactgtccgg gagcctccac
     5701 ttcatagagg taggtctcgt tgaaagtgcc atcacggtac agcgtagcct ggtaatcggc
     5761 aatgaccaga tcgccttcag tcaccatcgg gacggccagg gcaaggatac aggctcctac
     5821 cacaatgccg aatgcgaccg caatgaggat tgcaatctgt cgtttctcgt ccaaagggat
     5881 tcctcgctct ctagaactcg atctttggcg gggtcgctat tgcctcttca aactcgaggt
     5941 aggcgagttt tccgagaccg aggatgcgtg cgatgaagtt ggaggggatg acatccacca
     6001 gggtattgta ctgctgcgag atgttgttgt aggtgtatcg ctgccgcgct atctcctctt
     6061 ccacctcccg tactgcgtcc atcaggttca tcacggtcgt gctggtcttg agatccggat
     6121 aattctcagc tacagcaaag agcctcccga gaacggatcg cgactcgcgt tcgatctcgt
     6181 tgagatcacc tggacctgca ctcccgaccc gcgcccgcat ggcagtgacc ttttcaaaga
     6241 cctcccgttc aaactgtgca tagctcttca ccgagcccag cagctgctcg atcatatcca
     6301 gccgtttctt catcgcaacc ctgatctggc caagcgtggc ctccgacgaa tttttcaggc
     6361 tgaaaaaccg gttgtaaatc ccgataaagt agaggacaaa accgatcagg aggacgagaa
     6421 tcagccctcc gatgatccat ggagtgagac tttcccatgc cattctatcg attatagaga
     6481 tgagcaatca gtgtttaaag tattgtgtgg aaaaagggac catgccgtta ttcaaggacg
     6541 atcgtccctg ctacccgtag ttttcctccc tccaggtagt gcaggatcac acgtgccccc
     6601 ggccggtaga caagctcgga ctccgtacgc agcgtgataa ctggtttttt ccagtcgccc
     6661 gaattgtcta caaatgcgat ccttgcaggg atgaactgca tccaaagtcc ggcatggagc
     6721 accatcccct ctttgagcgg tgaaggccag tatctgacaa ggtttgccct gccgttgatc
     6781 agagaagaag aggtgatatc agtgcttgca gaaaggacat atccgcggtc cagatcctct
     6841 gtctctatcc cttttaaggc aagaccgacc cgatcaccag ggatcgcata ttccgcatca
     6901 tcatcatgct tctggatgga acggacgagg gcggtttttt ttgtcgggtg aaccctaagt
     6961 gtttcatggc ggcagatacg cccgccgatt acctctccaa ggacaaccac tccaatacct
     7021 ttcacattga agaagtgatc gaccgggact gcaccatggt ccctgatctg atcaggttct
     7081 ttcactgctt cgatttcggc aatgagttct tcccgtatcc tcatccggtc atcaggcaac
     7141 aggcggtagt gttcgacaac agttccctgg atcagcggag caatctgttc gggcgtaata
     7201 taattccgga ggacaaggat acctttccgt attcccgcac aatccagcat gatcagacat
     7261 tctccaaatg cagcattgat ctcgtcaacg acaagaattg cccgtgcggc catgctgacc
     7321 gagaagaaga gggaggcgag ccgttccgga tatcgtgctg gttcgagaaa tgtgatagtg
     7381 ttgtcgcctt ttttcaggtt gtaaaaagtg atgtcggaac tggttccttt ctttccgagg
     7441 tcttgggcat acccttccgg accaataacc gctaccgtaa gattggacat ctcctaactt
     7501 atctgaccgc tcataatatc agcatttatg tgcagcctga aatcctcccg gaaaaagaag
     7561 cgcagctacc aacccttcct ccgaatgaac aacggttgag gaatgctcga gcatgaggtt
     7621 cacatcctca tggtacccag ctttacgaaa atcggtccgg agtgatataa cggggatccc
     7681 tttcgaaaat gcatacccta tctcccatgc ggtaccagaa tctgcatccg gtccgtcaat
     7741 gaccgcgacc acaacatcgg agctgtcaag agcggcaaga ttctgcccga atatggcctt
     7801 gtgtgcatcc ctctcccttt tatgtgaatc atcccctgaa tcctgcggga ggtatacttc
     7861 ataacaattc ctacggagca gatctgcaag ctgaatgttg tacctccgtt ctgccaaaga
     7921 gaacaacggg gctgcaagat atacacggta ccgggaaaaa tctgatacgt ctattgcagg
     7981 gagatcgggg aaccgggaga gaaatgcacc cctcccccgt ctcctggcat caggggatcc
     8041 gcgggggccg taccaggtcg tattcacccc gcatgctgga gaggaattga cccccacgat
     8101 gcagagggga gggcctcgtt tcgatataat ttccctgacc ttctcttcga gcaaatcaag
     8161 aagatcagca aattcttttg tatccagacg gtcaagaaac acccctggtt cacggtcata
     8221 gccgagacag agagtctcag ggcaggggag gggtactacc tcaataccat actgataaca
     8281 tctttggatg gcacgggaga accattccag gtccttgtct cgggtgatcc ctttcgcacg
     8341 cagttgtggg tgaagaatgc aaggagagac cattacgtac attgatacca tgtggggatg
     8401 gaaagatgat agatgatccc actctacgcc ttccgggaca acagctgcga tccaaaaaaa
     8461 tgcaccgtga aacggctgga acgttcaggc ctggtacggg tactctccaa aatctcacac
     8521 ataccgagaa acaccatcct tcttgatccc actgcacccc aggcactttc acctgctgat
     8581 cgtcccgccc gttcgctgac tgctcttgat tgttcatggg aggtgctcga tacaggcgcg
     8641 gtcagttcct ggagaaagag gcgtgccctc ccgttcctcg tggcagcaaa cccggtaaat
     8701 ttcggaaggc cgtatcgtct cacttcggtc gaggcatttg gagccgccct tttcatcctc
     8761 ggggagcgcg agcaggccag gggggtactt gaaagccaga actgggggcc tcgttttctc
     8821 gaactgaatg aagaaccgct ctcgttatat gcaagggcgg gtgacagtac cgagataatc
     8881 gcgatacaga agatgtttct ttaatcctct tccaaccggc aatcgtatcg cacctctcaa
     8941 ccgggttcac ttcttatgat aaccagaatg tgtatacaat aatttccgga aaaaacctga
     9001 tcgtagtcag gtaagatagg agtagcacct agaattattc ggacttacca tttgcactcg
     9061 ggattgaaaa ggtagtattg caacctgtaa agtcagtcgt taagctttta gaaccatatg
     9121 ataaggggga atgggtgata ccactatcac gggaaatcag ggaacatcca tagatccctc
     9181 tcctgttgag gcataggata gcggagtgta ttgctgcaga atgagaattt ttccatctgt
     9241 tccctgaagt ccgaccgttc gcacctttac cggcagtacc ctgtgcgtgg tcagtggcat
     9301 aaggtgaacg ataagattgt tgttctccgg ttttgcatca tactccccaa ggatttgctg
     9361 ttcgaagcca gggaaaaaga tcgatacatg agccccgagg acaaactccc gctcaccttc
     9421 aacgattttg ataaaatttt catttgccca ccggacgatg cctgatgaat cgagaacaag
     9481 aattgcctca tcaacgagat tgaggatagc ctcaagttca tctcttgcta tcgatccttc
     9541 ctgcatctta ttctcttcac ccagaaaggg catcgtaccg atcgggtccc tcgtttcggc
     9601 atttttcagg gattcacggg cacgtttcca tgcagagata tcccggatcg attcaattgc
     9661 gccgatacgg tgcccttcgt tatcgacaag cgctgatgcc ttgccccaga gaaatgatcc
     9721 ctttcccccg ttcatatcag ggatataagc ctcaacatag atactgtcac cgaatctccg
     9781 gaccgagggg taatttcgtg caatctcatg gggtggcaga tccagaaggt tgacaaggac
     9841 cgggcgtgat cctttcaaaa aagagaatgc tttctggaaa tcctctctgc ccaacacttc
     9901 ctcccttttt actcctgaaa gtagctcgag tgctcggttc cacgcaatga tccggtgatt
     9961 ccggtcgatg atgaaggtag gatcaggcat aaattcgatt gtctcctcga gtttctgccg
    10021 ggaactcctg agggcagttt ctgcctcttt atggcctgta atatcaacga aggaagcgag
    10081 catatacagg agacgccctt ctccatttac cgttatccgg atcgatccct caagatacat
    10141 catgcttcca tccttctttc tcaccaccat ctcacctttc catctccctt tatctgacag
    10201 tgccctctct atctcttcga gtgagggaga gaccgtttcg aattgagcga aaatatcccg
    10261 tggctgtcgg ccccgtattt cattcatggg gatgccgaat tcttcgatga acgaatcatt
    10321 cgcatagatt aaccgctggt ttgcatcaaa gattgctatc gggttgatgg tggtcgcgat
    10381 cgcagtattc ttcagctgaa tctcttcctg tgctttttta aggtcggtca catctgcgaa
    10441 cgatgccatc atgcagaacg ggatccccaa ggtgtctttt accatccagg cagacaggaa
    10501 cgcataaaaa ggggtaccgt ctcttcttcg gggggtaacc tctccagacc aactcccttt
    10561 catcatgaga gaatcaataa cagtggtgat aatggcagcc tcttgtggat caccatggga
    10621 aaatgcttga atcggacgtc caataacttc tgcatcattc tgataaccga acaatctttg
    10681 atatgccttg ttggcgtagg taactactcc gttaaggtcg gctaatgcaa tgccattcac
    10741 tgatgtactc agtgcaaggt cttttaccac aagcgcttct tctgctttct tcctctcggt
    10801 gatatccctc ccgactccct gatattcgat aagagttccc tcggtattga atatggcacg
    10861 gttcgtccat tggagccacc gttgcgtacc gtccggaaat aacacccggt tttcgacggt
    10921 atcaacaggc ttctcgaacg agagtccctc gatgcaggta atgacccgtg ctctgtcatc
    10981 aggttgaata aattcaagaa attttttccc gttaaaatcc tcatttgtaa tcccataata
    11041 ctgtaatgct gccttgttta caaacaatat cctgagatcc ttgtcgaaac gcccgaccag
    11101 ttcggtctgg tcctcaacga tagcccggaa ctttgcttca ctcagtttaa ggcggtcctc
    11161 aaactgcttt ttttcagtaa tgtcctcaaa aattagcgtt gatcccctgg tcgcatcatc
    11221 aaaaacactc ggaaccgctt ttatccggag atagcaggtc tggccgtccc tggcatatga
    11281 cgtctctgtt attatttctc ctgaatcaaa cgcatccctt gcgagatctt cgatctttgc
    11341 agatccaagg ctgtttacgg ggcaggaacc gatagggcta ccaatggtgt cttctttttt
    11401 taatccaaaa aaggagagga aattttc
//
LOCUS       DAOOCO010000045        11415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5314_length_11415_cov_5.253169, whole genome shotgun sequence.
ACCESSION   DAOOCO010000045 DAOOCO010000000
VERSION     DAOOCO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_11415_cov_5.253169"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PK154_03920"
     CDS             <1..1464
                     /locus_tag="PK154_03920"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10243.1"
                     /translation="PGEIASVGENTTLGLVLNQTENGLSGCNISIALDNPAIATITGV
                     NFPAWASIQDNSTLPAQSVWMNAMDLQQTLTGNLTNVDLGSIALVGVLEGTTGITLTI
                     NALDDINGTAIPATTLPGTLVVHGPAVIAPTANFTVNTSSGTSPLTVQFADSSNGTEP
                     FSYEWNFGDGSPNVTTVDPVHTFVTDTQATYTITMTVNNSAGSSSTAQAITVNTTETT
                     NPPDSITNLHNTTYQEALITWNWTDPSSDTFDQVMIYLDGVFQANVTKGSGTFTAVNI
                     SPGTMHTLGTRTAGTTGLINETWVNHTAATAPQKDPSSFTVTLESGWNVFSTPILLTP
                     GHSQFSQIFSAGDQEKIIQALGWDGSLWYAAQATDEVTPLYAFYINVKEGEVANATIV
                     PSTSISSPPARALVTGWNLIGPAPAYEDGGFSPKPVEDSLITIEGDYRIVVSPGLHQP
                     GWSYVQGGASRDLLPYKGYWVFMDHPDTLAGFSTTPL"
     gene            1666..3369
                     /locus_tag="PK154_03925"
     CDS             1666..3369
                     /locus_tag="PK154_03925"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPW10244.1"
                     /translation="MRPPDLEYAADDQPEGPTLFLLGLQHTGIVATAFIFPVLVAKAA
                     GLEAGAAAFLVSMSMLANGIATIFQAVKHKEFGSGYLVPRVCGPNFVSASILAVQSGG
                     LSLLYGMTAVSGAFQIALSRIAHRLRVLFPVEVTGLVIMMLGVAVVPYALPNFFGMTA
                     TDPTPSGLATLISCITLAVIVGVTVWGRGHLKLFPLIIGMTFGYILCIAAGLAGADPL
                     GQIMASPIISFPDPQYFGVSFQTALIIPFCVAGLVTFVKSIGEFSICQRINDTEWKRP
                     DMMNIRAGLFADGIASTIGGLIGGMGQTGSSSNIGLSIATRSTSRYIGFVTGGILISL
                     AFLPVLAIVFLLMPGPVIGGSLIYVAGFIIVGGIQTITTRMLDSRKIFVIGISFIFGL
                     SVYLIPGAYETVPGFISPVFDSALSLATVVAIVLNLVMRLGIKKRVTTTIDLESWISD
                     KVFLFMERQGEMWAARKEVIHQMAMALTEACELITDHQMATGPLKVAVSFDEFNLDAE
                     ISYEGSPLTLPDKRPGEEEILSDPSGLLKLGAFLIQGYADKVTSGSDGSLAFLNLHLE
                     H"
     gene            3594..4757
                     /locus_tag="PK154_03930"
     CDS             3594..4757
                     /locus_tag="PK154_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPW10245.1"
                     /translation="MTPGIPRNIRKEHIISALATIDKDGYPKEHESIKYSLVYEGRKY
                     PPVWVIRTANLFANGEPLDPTLYSGGEQSNNFLKKFGFRIVKKTHSEVDHTSFDFETM
                     NRERIWREIQAKYKGQDIPAHVLRDTYRIYRGAGGLWVDKERTSKISNDDAGIAISIF
                     HKGTQYPDDLSDTAIRSPYPVTNRQGGQDGNEVKATKNAKRFNIPVFVITTSTLDPTR
                     RDVKKGFVRDWDDANQTFLIEFGKIPDYTKEGAFSEEIPFNLNRKRKRHLSSRSGSNP
                     RFSFDVFKRYGHCCAVCSMQIDGLLTATHIKPASENGTDDPRNGIPLCKNHHAAFEKC
                     FFTIHPGSYQIVTRENGPSKEELELVEEDILKLKALPERMALQWHYRRFLREI"
     gene            4908..5612
                     /locus_tag="PK154_03935"
     CDS             4908..5612
                     /locus_tag="PK154_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10246.1"
                     /translation="MTILLALDDTDTLSSRGTGRLARAIADLLGQEYPVLGVTRHQLY
                     VHESIPYTSHNSCAVIHIGPCGNGQEDRIFSLAREHMRADFVEGSDPGIAIAKQAAVT
                     PEVIAFGQDAKRMVLTQEQARALAKKSSICLEGLGGTEDGVIGALAGIGLAATGNDGR
                     FIQIGKIRTITGPTSAETLLDAGIDLILTLEGVPVTTGMIHVQEGKSVKPCPVSGRAV
                     LFVEKRNGTYRAVKRD"
     gene            complement(6121..6519)
                     /locus_tag="PK154_03940"
     CDS             complement(6121..6519)
                     /locus_tag="PK154_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10247.1"
                     /translation="MVRVAEYVVIRDGTFSIRIPGQEEHFEFSVPPEIVRPSADNALN
                     HRRPLLTYMVDPSANADVTLTVEINDQRIVRWTYKGGVGRMHQEVLSHEQLRVGENSI
                     QFRIDAIEGSHFQNVQISDVVIWFQKEVPV"
     gene            6862..7485
                     /locus_tag="PK154_03945"
     CDS             6862..7485
                     /locus_tag="PK154_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPW10248.1"
                     /translation="MLDMNKVKGFLMAPGETFRASKGDSLGSAFRYYTILLVIWTILA
                     AIVWLVMGYFAFQDIIIRIGNMGVFGSQVAEALTHFGAFVSTFQLFTVYCFFLLSLIG
                     IFFSAFIWHVFALLFGAKKEFTQTIKTTMYASTPFFLLGWIPYIAAIGVIWYLVLLIL
                     GLAEMQDMSVGEAAMAVVVPLLLLLIGAIIWGGVIYTLTMGILGIFT"
     gene            7757..8200
                     /locus_tag="PK154_03950"
     CDS             7757..8200
                     /locus_tag="PK154_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10249.1"
                     /translation="MAFDDTKTFQGKVYSGMSIGDHHLWEYPHGLWQEQKVAPDRWVF
                     SFRSKKKRVRKAPEGSGALPGTEYHWFILAHQKVRKLDQDTYETFMEGVKYKVAHKRP
                     SWRHWSTEYPDNEPEREILIRILEAYLADLKEGTGCQGCEKRPLP"
     gene            8267..8488
                     /locus_tag="PK154_03955"
     CDS             8267..8488
                     /locus_tag="PK154_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10250.1"
                     /translation="MIEIEIEESLKLIERYKKTLETVQSEDFQKVLKKHHMGNIPERC
                     TSSIYSKLLHLENEMSYLDSIVKGAKDLK"
     gene            8950..9420
                     /locus_tag="PK154_03960"
     CDS             8950..9420
                     /locus_tag="PK154_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPW10251.1"
                     /translation="MEEIAIVEVPDQQVLGITKIGSYTLIPHLLMQLVGYIMEKKYPF
                     AGMPTFLCHETSVECVKEANEKGTAKIEVVWPVAGKVSGSGDIRAYTLPGGKMVRTVH
                     RGPYDTCEPTYLALFAWIEDHHLQITGPIRELYPNDPREVKPEEILTEILVPVR"
     gene            9606..10001
                     /locus_tag="PK154_03965"
     CDS             9606..10001
                     /locus_tag="PK154_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10252.1"
                     /translation="MAPAEEQEDTGQGQGKPAKSDAVIMQSAVELVSTGRILVSVEGA
                     RIQSIREDLSVRCKGCRGPLRGSAEPIYTFARDWLRTSPEQRFEPDSPIIMHEYRYGN
                     YIPSPFWISTLRCRNCGMQTVVAVQLVVL"
     gene            complement(10154..10726)
                     /locus_tag="PK154_03970"
     CDS             complement(10154..10726)
                     /locus_tag="PK154_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW10253.1"
                     /translation="MELKGSKTEQCLIAAFAGESQARNRYTFFASVAKKEGYEQISAL
                     FQKTADEEKEHGKLFFKQLKGGSAVITAAYPAGMIGSTRENLLAAAEGEQHEWDTLYP
                     DFAGIAESEGFADIAALFRKVATVEAHHEQRYRKLLANVTKGTVFSRATPARWYCRNC
                     GAVFEGKQAPEKCPVCDHPKAHFELFSENY"
     gene            complement(10958..11197)
                     /locus_tag="PK154_03975"
     CDS             complement(10958..11197)
                     /locus_tag="PK154_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10254.1"
                     /translation="MRKRERELHQVRHVHLNDIAIYRDIPEAAWRSIDSQLFRIREAN
                     HPWHECMVPIEPRQSGRGEQELPLALANQHSRDTD"
     gene            complement(11215..>11415)
                     /locus_tag="PK154_03980"
     CDS             complement(11215..>11415)
                     /locus_tag="PK154_03980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW10255.1"
                     /translation="AGPNTGGSQFFINLVNNNYLDTKHPVFGKVVEGMDIVDAIGKVR
                     TTGRAGGDRPLQNVTIIKAEMI"
BASE COUNT         2699 a         3131 c         2943 g         2642 t
ORIGIN      
        1 ccaggcgaaa ttgcttcggt gggtgagaat acgacacttg ggctggtcct gaaccagacc
       61 gaaaacggcc tctccggctg caatatcagc atcgcactgg acaaccctgc tatcgcgacc
      121 attaccggag tgaacttccc ggcctgggcc tccatccagg acaactccac gctcccggca
      181 cagagtgtct ggatgaatgc tatggatctg cagcagaccc tcacgggcaa tctcaccaac
      241 gttgaccttg ggtccattgc tctcgtggga gtcctcgaag gaacaaccgg gatcaccctg
      301 accatcaacg ccctggacga tatcaacggg actgcgatcc cggcaacaac gctcccgggc
      361 actttggtcg ttcacggccc ggccgttatc gcaccgaccg cgaatttcac ggtgaacacc
      421 tcgtcaggaa cttcaccact cacggtgcag ttcgctgaca gctcgaacgg cactgaaccg
      481 ttctcctacg agtggaactt cggagacgga agcccgaatg tgaccacggt agacccggtt
      541 catacctttg ttaccgacac tcaggcgacc tataccatca caatgacggt gaacaacagt
      601 gctggatcaa gctcaaccgc acaagcaatt accgtcaaca ccacggaaac aacaaatccc
      661 cctgacagta tcacgaacct tcataacacg acctaccagg aagccctgat cacctggaac
      721 tggaccgatc cctcatcgga cacctttgat caggtgatga tctatctgga tggggtgttc
      781 caggccaacg tgacgaaggg atccgggacc ttcactgctg tcaatatcag tcccggaacg
      841 atgcataccc ttggcacaag gacggctgga accacaggac tgatcaatga aacctgggtc
      901 aaccatacgg cagccacggc tcctcagaag gacccgtcat ccttcactgt caccctggaa
      961 agcggatgga acgttttctc gacaccgatc ctgttgacac cggggcattc ccagttctcc
     1021 cagatcttct cagccgggga ccaggagaag atcatccagg cactgggatg ggatggatcc
     1081 ctctggtatg ccgcccaggc aaccgatgaa gtgacccccc tgtatgcctt ttatatcaac
     1141 gtaaaggagg gggaggtggc aaatgcgacg attgtgccat ccaccagtat atcgagtccc
     1201 ccggcccggg ccctcgtgac cggatggaac cttatcggtc ctgcaccggc gtatgaggac
     1261 ggaggctttt caccaaaacc ggtcgaggat tcgttgatca cgatcgaagg ggattaccgc
     1321 atcgtcgtca gcccagggct gcaccagccc ggctggtcgt acgtgcaggg tggggcatca
     1381 agggatctcc ttccctacaa gggatactgg gtattcatgg accatcccga cacccttgcc
     1441 gggttctcca ccacccctct ctgatttttt cagaggcagc ttcaggatct tcccggagcc
     1501 tcttgagaac agacggttaa ttagactttt tttccttcaa aggccgcctg ccgttgccgg
     1561 ggatagtcat cgggaaccgc gagtcaggaa tgtgaatcag ggcctcctgc tcaccatact
     1621 acaatcataa cctttatcaa aatcctgtgg cctttgttcg tgtgcatgcg gccaccggat
     1681 ctcgaatatg ctgctgatga ccagcccgaa ggccccacgt tgttcctcct cggcctgcag
     1741 cataccggga ttgtcgcaac cgcctttatt tttcccgtcc ttgtcgctaa ggctgccggc
     1801 ctggaggcag gggcagcggc ctttctcgtc tccatgtcga tgctcgcaaa cggcatcgcg
     1861 acaattttcc aggcggtgaa acacaaggag ttcggctccg gatacctcgt cccccgggtc
     1921 tgcgggccga attttgtttc agcctctatc ctcgccgtcc agtcgggggg cctctccctg
     1981 ctctacggga tgactgcggt ctccggggcg ttccagatcg ccctctcccg cattgcccac
     2041 cgtctccggg tcctcttccc ggtcgaagtc accggccttg tgatcatgat gctcggagta
     2101 gccgtcgtcc cgtatgcctt gcccaacttc ttcgggatga ctgcgacgga tcctactcca
     2161 tcgggactcg caacgctgat ctcctgcatc accctggctg ttatcgttgg agtgaccgtt
     2221 tggggcaggg gccatctgaa gctgttcccg cttatcatcg gtatgacctt cgggtacatc
     2281 ctttgtatcg cggcaggact tgccggtgca gacccgctcg gccagatcat ggcatcaccg
     2341 atcatctcgt ttcctgaccc gcagtacttc ggggtctcct tccagaccgc actcatcatc
     2401 ccgttctgcg ttgcgggtct cgttaccttt gtcaaaagta tcggtgaatt ctccatctgc
     2461 caacggatca atgataccga gtggaagcgg ccggacatga tgaatatccg ggccgggctc
     2521 tttgccgacg gcatcgcatc aaccataggc ggtcttatcg ggggcatggg acagaccggc
     2581 tcctcctcca atatcgggct ctccatcgcc acgcgttcga cgagccggta catcgggttt
     2641 gtcacgggag ggatcctgat cagccttgcc ttcctcccgg ttcttgcgat cgtgttcctc
     2701 ctgatgccgg gtccggtgat cggcgggagc ctgatctacg ttgccggctt catcatcgtc
     2761 gggggtatcc agacgatcac cacgcggatg ctggactccc ggaagatctt cgtaatcggc
     2821 atctcgttca tcttcgggct gagtgtgtac cttatccctg gtgcgtatga gaccgtcccc
     2881 ggatttatca gcccggtatt tgattcagca ctctcgcttg ccaccgtagt cgccatcgtt
     2941 ctgaaccttg tcatgcggct ggggatcaaa aagagggtca ccaccaccat cgatctggag
     3001 agctggatct ccgacaaggt cttcctcttc atggaacgcc agggggaaat gtgggcggca
     3061 agaaaagagg tgatccacca gatggccatg gcactcaccg aggcatgcga gctgatcacc
     3121 gaccaccaga tggcaaccgg ccccctgaag gtggcggtat cgttcgatga gttcaatctg
     3181 gatgccgaga tctcgtacga gggcagtcct ctcacgctcc ctgacaagcg tcccggcgag
     3241 gaggagatcc tctccgatcc ttctgggctg ctcaagctgg gggcatttct catccaggga
     3301 tatgccgaca aggtgacgtc tgggtcggat ggttcgctcg cgttcctgaa ccttcacttg
     3361 gaacattaag tacttgtgag cgaaccggat cgcctcatca tgaggcattt tctgcttcag
     3421 cttcgggtat gccgttctgt tttcactttt cagaggtgac agcatccgga tcttttcggt
     3481 ccgcttcctg tctcttaagg ggtttccctg acggagagtt gagagcggtg tcttttctcc
     3541 cccatcaaaa ccgtcaccct tactataaga tcaaccaact atttcacaga gcaatgacac
     3601 ccgggattcc caggaatatc aggaaagaac atatcatctc cgcccttgca accattgata
     3661 aggacgggta tccaaaagag cacgaatcca tcaaatattc cctggtatac gaggggagaa
     3721 aatacccgcc ggtatgggtc atccggacgg ccaatctctt tgcgaacggg gagccccttg
     3781 atcccaccct ttattccggg ggagaacagt ccaacaattt tctcaagaag ttcgggttcc
     3841 ggatcgtgaa aaagacccat tcagaggtgg atcataccag tttcgacttc gagaccatga
     3901 accgggagcg aatctggagg gagatccagg cgaagtataa aggccaggac atcccggctc
     3961 acgtcctgag agatacctac cggatatatc gtggtgccgg agggctctgg gtggacaagg
     4021 aacggacctc aaagatctcg aacgatgatg cagggattgc catctccata ttccataaag
     4081 ggacacagta ccccgatgac ctttcagata ccgcaatacg ctccccgtac ccggtgacca
     4141 accggcaggg cggacaggac gggaatgagg taaaggcaac aaagaatgca aagagattca
     4201 acatcccggt gtttgtcatc accacctcta cactggaccc aacaaggagg gatgtaaaga
     4261 aaggctttgt ccgggactgg gatgatgcga atcagacctt cctgatcgaa tttgggaaaa
     4321 tccctgacta cacaaaggag ggggctttct cggaagagat ccctttcaac ctgaaccgga
     4381 aaagaaagcg acatctctca tccaggagcg ggagcaatcc aaggttctcc ttcgatgtgt
     4441 tcaagcggta tggccattgc tgtgcggtat gctcgatgca gatcgacggt ctcctgactg
     4501 ccactcacat caaaccggcc agcgaaaacg gaaccgatga cccacgaaat gggattcccc
     4561 tctgcaagaa tcaccatgca gcgtttgaaa aatgcttttt caccattcac ccaggatcgt
     4621 accagattgt gacgagagag aacggacctt caaaggaaga gttggaactc gtggaggagg
     4681 atattctgaa attgaaggca ttgcctgagc gaatggcgtt gcagtggcat taccggcggt
     4741 ttttgaggga aatttagata cctcgctctg gcattgcttc tcatctttcc tcaagtgaat
     4801 caccaataca tgccggtgtt caccagtaca ctcatcgaat tcccttaaca aaagaaaatg
     4861 gaaagggtat tacgttcacc atgagaacct gagagaagca ctctaaaatg acaattctcc
     4921 tcgctctcga tgataccgat accctctctt cacggggcac cggtcgcctt gcccgtgcga
     4981 ttgcagacct gctcggacag gaatacccgg tgcttggggt gacccgacac cagttgtatg
     5041 tacacgagtc cattccctat acctctcata acagctgtgc agtgattcac atcggtccct
     5101 gtgggaacgg acaggaggac aggatctttt ccctggcacg tgagcatatg agagcagatt
     5161 ttgttgaagg aagtgatcct gggatcgcaa tcgccaaaca ggcagcagtc actcctgagg
     5221 tgatagcctt cggccaggat gccaaacgaa tggtcctcac tcaggagcag gccagggcac
     5281 tcgcaaaaaa gagctcgatc tgcctggaag gactcggagg aacggaagac ggggtgatcg
     5341 gtgccctggc ggggatcggg cttgctgcaa cgggaaacga tggcaggttc atccagatag
     5401 gaaagatccg cacgattacc ggccccacca gtgcagaaac actgctcgat gcgggaatcg
     5461 acctgatcct cacccttgag ggcgtcccgg tcaccaccgg catgattcat gtccaggagg
     5521 gaaagtcggt caaaccctgc ccagtttcag ggagagcggt gctctttgta gagaaaagga
     5581 atggaaccta tcgagcggtg aaacgcgact gagcaagtgg tacaaataga gagaggcctc
     5641 ttttcatgac gccaatcgtt gtttccaggg agtgcgttct tcgctggcag atgccacgga
     5701 aaaaacagga attgatatta catccttgtt caccccggat tccctgacaa gatatcaggc
     5761 attcctctcc tttctcctgt cgtccgggaa tgtggaactg tgtgtgaaaa agagagagta
     5821 ccgtttctta ccgacttagg gataaaagaa acctggtagg tggagataaa aaatcattta
     5881 cttccccgta aaaatcctta ctccccctta atggtacaac agatggatgc accgatatga
     5941 ttcactaaaa attcctgtag aatagaaaaa gaggagaagc aagggtacct ctggaccctt
     6001 tcttgggaat cgtcacatgg gctctcccct gctggcttgc acaggcagct ttcgaatccg
     6061 ctgctccctc cccgtacgga tgatctcagg aacgccgttt atgctttcat atttcaagga
     6121 ttagaccggt acctctttct ggaaccaaat gacaacatca gatatctgga cattctggaa
     6181 gtggctgccc tcaatagcat cgatcctgaa ctggattgag ttttcaccga cacgcagctg
     6241 ctcgtggctc aggacctcct gatgcatcct gccaacccct cctttgtacg tccagcggac
     6301 gatcctttga tcatttatct caactgttag agtgacatct gcattagcac ttggatccac
     6361 catataagtg aggagaggcc ttctatgatt gagagcatta tctgcagaag gacggacaat
     6421 ctcagggggc acactgaact caaaatgctc ttcctgacca gggatacgga tactgaacgt
     6481 gccatctctt atgacaacat actctgctac tctgaccatg atgagccatt ataaagccta
     6541 acccataaac ttaccttctc caccaggata cgatcagcat agtagactgg cttcaggtca
     6601 ttgcactgct catctgtggc cgtcaggatc gagacaactg ttgcctccat ttccctgaca
     6661 taatcactga ttatcgatga aatgccagaa aggaatgcga gatggaagtt ctaataaaag
     6721 aaatctggtt cccattcctt catcaggaag ctcgtgctct gagctcgtaa gaggatattt
     6781 tcgaaacttg tattctcttc cgttcacaat cgactatcct taccatatct ataaatacca
     6841 agagatagga tcagccgtta tatgcttgat atgaataaag tgaaaggatt tctcatggct
     6901 cctggtgaga cgttccgggc gagcaagggc gattccctgg ggagtgcatt tcggtattac
     6961 accatactcc tggtgatatg gaccatcctt gctgccatcg tctggctggt gatgggatac
     7021 tttgcgttcc aggacatcat catccggatc gggaacatgg gggtttttgg tagccaggta
     7081 gcagaggcgc tcacccactt cggagcgttc gtctccacct tccagctctt tacggtatat
     7141 tgtttcttcc tcctgtcgct cataggtatc ttcttctctg ccttcatctg gcacgtcttt
     7201 gcccttctct tcggggcgaa gaaagaattt acccagacca tcaagaccac gatgtacgcc
     7261 tccaccccct tcttccttct cgggtggatt ccgtatatcg ctgccatcgg cgttatctgg
     7321 tacctggtgc tccttatcct cggactcgct gagatgcagg acatgtcggt aggcgaggca
     7381 gcaatggcag tcgtggtgcc cctcctcctg ctgcttatcg gggcgattat ctggggtgga
     7441 gtcatctata ccctgacgat ggggatactg ggaattttca cgtaagggat cccaaaaaaa
     7501 gagtccaatc aggatatgat ttccccccac attgaaaaaa tccaggcggg gattacccta
     7561 tcctgaggcc tgactcactc tcattcgaac tcgtggtatt cattttctgt ctaaataacc
     7621 aataagcggg cttctgttgc gcgaatgggt tgatatctcc ccaaaccggt tcagatttat
     7681 cctccccatg agtgaaaagt tcactttcac cctccgttcc cgtaaaggaa ttgaagcatg
     7741 aaacacagga tcacctgtgg catttgatga tacaaagacc ttccagggga aggtctattc
     7801 cgggatgtcc atcggtgacc atcatctctg ggagtacccc catggcctgt ggcaggagca
     7861 gaaggtggcc ccggaccgct gggtcttctc cttccgatcc aagaagaaga gggtccggaa
     7921 agccccggaa ggatcaggag ccctccccgg gaccgagtat cactggttca tccttgccca
     7981 ccagaaggtc cggaagctgg atcaggatac gtacgagacg ttcatggagg gggtcaagta
     8041 caaggtggcc cacaagcggc cctcctggcg gcactggagt actgaatacc cggataatga
     8101 gccggagcgg gagatcctga tccggattct cgaggcgtac ctggcagacc tgaaggaagg
     8161 gacaggctgt cagggatgtg aaaaacgacc gttaccgtga gctcgacaca gcctttaaat
     8221 actctgactg aagaatccgt atcagaagag tccaggacat gataccatga tcgagatcga
     8281 gatcgaagag tccctgaaac tcatcgaacg gtacaaaaag acactggaaa cagtccagtc
     8341 ggaagacttc caaaaagtgt taaagaagca tcatatgggc aatatccccg agagatgtac
     8401 ctcatctatc tattccaaac tccttcacct ggaaaatgag atgagttacc tggattctat
     8461 tgtaaaaggt gcaaaagacc tcaaataata aggcttaaaa ggatctttgg tggagggata
     8521 tcttcgtaat cacacgaagg taatcctcca tgatctcttc ttcgcattat gagttctttg
     8581 cattatgagt tcttcgcatt atgaggtatg caactttttt cattctctga tgcccggtga
     8641 gaaacggtac cgtattgtat tggtccgata tggccgcttg cacctgaaga gctgaaaaaa
     8701 cgctgccgat gactcttgca tgaggattga aataaataac atgattcctt tgaaggcgac
     8761 aagcataaaa gatgaaaatg gcggaggcat ttccacataa aaatgagaac catccaggcg
     8821 atctcttccc atcgggaaaa taccactcat catcacctca atccttcctg taaaatgacg
     8881 aacacggaca cgttcgccct cccgggtcat atctttataa acccggcacc tgatcggtta
     8941 ttgactcata tggaagagat tgctattgtg gaagtacctg accagcaggt actcgggatc
     9001 acaaaaatcg ggagttatac gctgatccca cacctcctca tgcagcttgt cgggtatatc
     9061 atggaaaaga agtatccctt tgccggtatg ccgacattcc tctgccatga gaccagcgtg
     9121 gaatgtgtaa aagaggcgaa cgagaaaggg acggctaaaa tcgaggtcgt ctggccggtt
     9181 gcgggcaagg tctccggcag cggggatatc agggcgtata ccctcccggg ggggaagatg
     9241 gtacggaccg tgcaccgagg accgtacgat acctgtgagc ccacctacct cgccctcttt
     9301 gcatggatcg aggaccatca cctccagatc accgggccca tccgcgagct ctaccccaac
     9361 gatccccggg aagtgaaacc cgaagagatc ctgacagaga ttctcgtgcc agtgcggtga
     9421 ttcaacctct tttttcgatg aacgggaagg gacgcctggc gtctcttcca gaatcctcct
     9481 ccgtttcgta catcacctcc aaatacagcg tctttcggct gatgtatgaa ccctctcacc
     9541 ctgtttccgg ttcatccgtt tttggaaggc aaaactgata aaagagtgcc ggataatgat
     9601 cacgcatggc gccagcagaa gagcaggaag atacagggca gggacaggga aagccggcaa
     9661 aaagtgatgc agtgatcatg caatctgcag tggagctcgt cagtacgggc cggatccttg
     9721 tcagtgtgga gggggcacgg atacaatcca tccgggaaga cctttccgtt cgttgcaagg
     9781 ggtgccgggg acctttacgg gggagtgccg aaccgatcta tacctttgcc cgggactggc
     9841 tccgtacgag cccggaacag cgatttgaac ccgattctcc gattataatg catgaatacc
     9901 ggtatgggaa ttatatccct tccccgttct ggatcagcac tctgagatgc aggaactgcg
     9961 ggatgcaaac ggtcgttgct gttcagctgg ttgtcttgtg agggagaaag cctgggatgg
    10021 ggaaagatcc cctctgttcc cctttttccc atgagaggca attcaccaac cgccccttct
    10081 ctcaaccaga ctcgtcatcc ggccattcca ttgagagagg agagaatgat aaaaaagaag
    10141 aaatgcccct ctttcaatag ttctcggaga agagctcgaa gtgggctttc gggtggtcgc
    10201 agaccgggca tttctcaggg gcttgcttcc cctcgaagac cgcaccgcag ttccggcagt
    10261 accagcgtgc cggtgtcgca cgggaaaaaa cagtcccctt ggtgacgttt gcgagcagtt
    10321 tccggtaccg ctgctcgtgg tgtgcttcga ccgttgccac cttgcggaag agggcagcga
    10381 tatcggcaaa tccctccgat tctgcaatcc cggcaaagtc cgggtagagc gtgtcccact
    10441 catgctgctc gccctcggca gcggcaagca ggttctctct cgtcgagccg atcatgcctg
    10501 cggggtatgc ggcggtgatc acggcactcc ctcctttgag ctgtttgaag aagagcttgc
    10561 catgctcctt ctcctcatca gcagtcttct ggaagagggc tgatatctgc tcatatcctt
    10621 ctttttttgc aacgcttgca aagaaggtat accggttacg ggcctgggat tcgccggcaa
    10681 atgcagcgat caggcattgt tctgtcttgc tccctttcaa ctccattggg atctcctgaa
    10741 gagacatgct ccccgatcaa cgatatacac agctgactgg atccctgaag cagggaaaca
    10801 taccctggaa ttgaaagtgc cacatggtct ccggaaataa caggtggtat cgcattgcag
    10861 tggtcctttt ttccgactac cctggtagtg atacaccacg ggtataatct tccgcccttg
    10921 tgaggaagcg gtgtaatgaa gcggggagaa tccgggatca atcggtatcc ctggaatgct
    10981 ggtttgccag ggcgagcggg agctcctgct ctccccgccc tgactggcgt ggctctatgg
    11041 gaaccatgca ttcatgccat ggatggttcg cctcccgtat ccggaatagc tggctatcta
    11101 tggaccgcca agccgcctct ggaatatcac gatagatcgc gatatcgttc agatggacat
    11161 ggcgaacctg atgaagctcg cgctccctct tcctcatttt ccttaaaaaa tggatcagat
    11221 catctcagcc ttgatgatcg tgacattctg gagcggccga tctccaccag cccggcccgt
    11281 cgtccggact ttgccgatcg catcgacaat gtccatcccc tcgaccactt tcccgaagac
    11341 cgggtgcttt gtgtccaggt aattgttgtt gacgaggttg atgaagaact gggatccgcc
    11401 agtgttggga ccggc
//
LOCUS       DAOOCO010000046        11223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5426_length_11223_cov_4.656877, whole genome shotgun sequence.
ACCESSION   DAOOCO010000046 DAOOCO010000000
VERSION     DAOOCO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_11223_cov_4.656877"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK154_03985"
     CDS             complement(<1..235)
                     /locus_tag="PK154_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPW10256.1"
                     /translation="MALRFADAGVAAIIHTDISRDGAMGGANVEASARLAEAGGIPVI
                     VSGGVAGIDDIRRVAERKTSGLAGAIAGRALYDG"
     gene            236..838
                     /locus_tag="PK154_03990"
                     /pseudo
     CDS             236..838
                     /locus_tag="PK154_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
     gene            825..2108
                     /locus_tag="PK154_03995"
     CDS             825..2108
                     /locus_tag="PK154_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPW10257.1"
                     /translation="MYPIEEIFRKGAVLADEVEVYLTEGHSLSGELKRAQVSRVAESR
                     YFSLSVRTIKDGRIGNSQSSNPRDWEKCLDASVASGTLATEQPWNGLPHPAKLDSRPI
                     AFDPSLTPDSDIILGLLKGMIEGASSHPVEVTSGGASLSVQTTTLANSNGLFYSRPRS
                     HVSASIETIRENSTGYEFGNSYTMDLDPVSIGDRAGFLASASYGGIDISSGPYDVVLS
                     PVAFAQLIGAVLLPALSGRNVHAGRSKLATSLGDQVIDPALSLSDDPFLEKGLGSTRW
                     DAEGVPTRHLSFIRKGVLEHFSYDLKTASRYGKESTGSAVRTGHGGGPAIGNHNVIID
                     GPRMDVMEDEAIYIQDVVGAHTANPLSGDFSVELTNPFRISGGTYEKPVRKAMMSGNV
                     FDMLGDTSGLGRDDRVIGSIVVPSIRLKKQHIIGT"
     gene            2110..2376
                     /locus_tag="PK154_04000"
     CDS             2110..2376
                     /locus_tag="PK154_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HPW10258.1"
                     /translation="MSFDLALIVAGILIAVVMIFLLKDIVKIIINSILGLLLLFFINY
                     FDLLGYVGRPDIPYTPVNILLCALGGIPGALIVIILQLTGITIT"
     gene            2538..3044
                     /locus_tag="PK154_04005"
     CDS             2538..3044
                     /locus_tag="PK154_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW10259.1"
                     /translation="MEEAEYCLDDRRTLLLLKGPSSFILAGKAGSRFPLCVEYGDGEI
                     CTTIEKTDIIAVSAPEGGALEPAVMLMELVRAHHVPLLVLPQGHPGSKRLRYVVSAGP
                     EISLSCGIQRGTHPDQHLLCSSGELAGILLSGTMEGIRVASMPSSVTALILTHSLTIG
                     TKALRGGS"
     gene            3312..3641
                     /locus_tag="PK154_04010"
     CDS             3312..3641
                     /locus_tag="PK154_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW10260.1"
                     /translation="MKTEVLKHIKATEDEYQSLINTVMAERKRILADAELEADNLIMK
                     ASTSAEEYRKMRLAEARAQAERKHAEIVREGERKSADLKEKGTQNLNSAVELLVSRFK
                     ESLHAHP"
     gene            3628..5601
                     /locus_tag="PK154_04015"
     CDS             3628..5601
                     /locus_tag="PK154_04015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPW10261.1"
                     /translation="MLTPEQMSKVLIAGPKDALDPVIAELHRHRLFHIEDFVENDQEE
                     YTGYRIGNSLEGAGDISKELLRLRSVTGAFSLRADDLDPKARIRRSELGAAIEKELPR
                     IEDEVESLLTRKTAIENHMKETEQKIEALTPFADLRTDLSLLHGYRTLTFFAGTIAKK
                     IDLGVPSEVYYSESKKGNFLVAAVPAEYRQEVERKLLDAQFQAVPIPDEQGPARERIS
                     VYNVQLETDKKDIEEIASQINALKSQHSEFLVACDELLSIEAEKAEVPLRFATTDHAF
                     VAEGWVPTESLEGLVKGITTASGGKAYVTQLPVEEGKDLPPMEYNNVNFVKPSQFLID
                     VYSRPKYNELDPTLIVSIIFPIFFGLILGDVGYGAILLAMSLGLRMVIKGDDGRMLLD
                     VLRNASISSIIFGFLYSEFLGFPIPGLHPLLPTRHLNIGGEAGGHGPAVPELMIMAIW
                     IGIAYITLGRILGMINHARQDHGSHRVKAVLANLGWIMVMWGILLMIWSFFAMPLMPD
                     LTGYPPLVAGLSMPALAGAVMILVGILFIARDSALEIVELPTILSHVLSFARLVGVGL
                     SSVAIAMVVNFIAIGLIIEPQLERLTIIGVIIIIVGVFVFLLGHLFNAILGLLGGGLQ
                     SLRLNYVEFFTKFYKGGGKKYNPFGLKRRFTED"
     gene            5605..5862
                     /locus_tag="PK154_04020"
     CDS             5605..5862
                     /locus_tag="PK154_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW10262.1"
                     /translation="MVGEELVGLTPEMIEASQAGMKALGAGLAVGLTGIGTGLAEMAI
                     GSAAVGATAENKDVFGLVLLLTVIPETIVIFGLVVGLLLLF"
     gene            5878..6456
                     /locus_tag="PK154_04025"
     CDS             5878..6456
                     /locus_tag="PK154_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPW10263.1"
                     /translation="MGLEVVIDDIREKGKREGDRIKKETDAEVGSILMTAQEKAERIK
                     LAAEQDVQKQVEHITNQEASAANLVVKRQLLNTQKELLDQVYSAALSRIAALPEDFHR
                     DTIKTLLSRARAEVPEGIVHCSRRDSELLSRILSSDTSLKGYTKGDPVEIEGGVIIES
                     KDGELQLDYSYRTFLNGVWETGLKDASGILFR"
     gene            6468..7526
                     /locus_tag="PK154_04030"
     CDS             6468..7526
                     /locus_tag="PK154_04030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HPW10264.1"
                     /translation="MADISAGPAPYVYVCTRLRVRKAKLIPREDYLRLLNMSIPEITR
                     FIEETQYKREIDELASSFHGIDLIELALSWNLAKEYQSIQDITPGVLKRFTQSYLRKW
                     DIQNILTILRGKTQGMKPGKIREILVPAGEMDRVFLDRLVKEETPERILEALKGQSVY
                     PAIAAGFSTAVSEGSFARIENELYKQFYENLLSEATSGFKGGKQFLEYIMLDIDITNI
                     RNMFRLRADTLHEDARSMMIPGGTFSTDAFQHLISIEESKEFIDTLKSKVHVKPLIML
                     LEEMSGGEKSLREIEIDLIKVQLAQMESLSKLNPFSIHPILTYLEKKKYEIFNLRAIA
                     RGKEVNLPTDRIRSYLVM"
     gene            7528..7830
                     /locus_tag="PK154_04035"
     CDS             7528..7830
                     /locus_tag="PK154_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPW10265.1"
                     /translation="MEIAVVGSSDFVLGFRLSGIRRTYAAESDEKLAEYITRILDDGT
                     IGILVLQSRDMNRIPRRLQTTLENSVKPTVIAIGGEEGGMSMRERIKRSVGVDLWK"
     gene            7821..9584
                     /locus_tag="PK154_04040"
     CDS             7821..9584
                     /locus_tag="PK154_04040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit A"
                     /protein_id="HPW10266.1"
                     /translation="MEVTEKKEKGQEGVLKRIAGPVVTAVNIDAHMYDVVKVGHEELM
                     GEVIKIQGDETIIQVYEDTSGIRPGEPVTNTGLSLAVELGPGLLTSIYDGIQRPLSVL
                     VDKMGDFIQRGVSAPGLDHQKKWEFRPLVKEGDKVSPGMVIGEVQETNIVHRILVPPK
                     VQGGVVKSIKRGSFTIEEVVCTLDSGTELTMMQKWPVRVPRPVTEKMNPTIPLITGQR
                     ILDGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTE
                     FPELEDPKSGKPLMERTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYDVSLMADS
                     TSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFYERAGLVRTLSGKNGSVSVIGAV
                     SPPGGDFSEPVTQNTLRIVKVFWALDAKLSQRRHFPAINWLNSYSLYLDSLHDWYDKE
                     VSPEWNVLRSWAMEVLQKEAELQEIVQLVGSDALPEAEQLTIEIARMIREIFLQQNAY
                     DAVDTFCDMSKQYDMMKAIKGYADLAYAAQTAGVSPAQIITIKSKNDLPQIKFIKDYK
                     PELERIQKSMDSEINTLRSTA"
     gene            9581..10972
                     /locus_tag="PK154_04045"
     CDS             9581..10972
                     /locus_tag="PK154_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW10267.1"
                     /translation="MKEYRTISKIAGPLVFVEKTEPVGYNELVNIALWDGTIKRGQVL
                     DTSDSLVVVQVFETTAGIGKDSGVRFLGETIKMPVGKEMLGRILSGGGKPIDGGPEIV
                     PEKRLDITGAAINPYARASPEEFIQTGISTIDGTNTLVRGQKLPIFSGAGLPHNNIAL
                     QIARQSKVLGSKESFAVVFAAMGITKEEANHFMQDFERTGALERAVVFLNLADDPAVE
                     RIITPRLALTTAEYLAFDLDMHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLAGIYERAGIIKGKKGSITQFPILTMPGDDITHPIPDLTGYITEGQIVVSRELHRK
                     GIYPPINVLPSLSRLMNLGIGKEHTREDHKKVSDQLYAAYAEGNDLRGLVAIVGKDAL
                     SERDRMFLEFADLFENRFVRQGYDEDRTIFETLDGGWNLLATLPVEQLVRIDRDLINK
                     YHPKFRQGAKAQE"
     gene            10979..>11223
                     /locus_tag="PK154_04050"
     CDS             10979..>11223
                     /locus_tag="PK154_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW10268.1"
                     /translation="MALRDIKPTRSELINLKRRIVLSERGYKILKMKRDGLIIEFFKV
                     LEKAKDSQSDLQQKYERATQMMAFANTVEGAIGVKSAA"
BASE COUNT         2642 a         2985 c         3246 g         2350 t
ORIGIN      
        1 tgccgtcata gagcgcccgt ccggcgatcg ccccggcgag gccgctggtc ttgcgctcgg
       61 cgacgcggcg gatgtcgtcg atgccggcga cgccgcccga aacgatcacc gggatgccgc
      121 cggcctcggc gagccgggcg gaggcctcga cgttggcgcc gcccatcgcg ccgtcgcgcg
      181 aaatgtcggt gtggatgatc gccgccaccc cggcgtcggc gaagcgtagc gccatctcct
      241 cggcgggaag atcggagagc ggatcgggag cccgatcgtc accatcgtcg atgatccctc
      301 cctccccgaa ttcggattcg aaccctgcga tgccgagggc tcgcgggtgg aacgcaccga
      361 gatcatccga aaaggggtgg ttgcagggtt cctccattca cgggagacgc tcgctgcggt
      421 cggaaacggc cattccggga atgcccgggc gatgcccggc gaacctcctc ttgtgaggat
      481 gagcaacacc ttcattgaag cgggagagag cagcaccgat gaactgatcg ctgaatgccg
      541 gaccggcatc cttctcaaag gctcccgtgg cgggcaggtc gatccgggaa ggggggtctt
      601 ccagttcaat gcagagtacg ggtacctcat cgagcatggc gaatgcacca gcatggtccg
      661 ggatgtctcc ctttccggtg agatcctgca gacgctccac gctatccggc tctgttcgaa
      721 cgatcggctg atgcaccagg gctattgcgg aaaaggaggc cagagcgtcc cggtgagcga
      781 cggtgcaccc catgtcctcc ttgctgatgc agtggtaggt ggccatgtat ccgattgagg
      841 agattttcag gaaaggtgcg gttctcgctg acgaggtgga ggtctacctc accgaggggc
      901 actcactctc gggagagctg aaacgtgcac aggtgagccg ggtcgcagag tcgcgatatt
      961 tctcgctgtc ggtcaggacg ataaaggatg gccggattgg caattcccaa tcgagcaatc
     1021 cgcgtgactg ggagaagtgt ctcgatgcct cggtcgcaag cggtaccctt gcgacggagc
     1081 agccctggaa tgggctcccg catcctgcca agctcgattc ccggccgatt gcattcgatc
     1141 catcgcttac ccctgatagt gatatcattc tcgggctcct gaaggggatg atcgagggtg
     1201 catcatcgca tcctgttgaa gtgacctctg gaggggcttc cctctccgtt cagaccacta
     1261 ccctcgcaaa cagcaacggt ctgttttact cccgtccccg gagccatgtc agcgcttcta
     1321 tcgagacgat acgggagaat tcaacgggtt acgaatttgg aaattcctac acgatggatc
     1381 tcgatcccgt ttcaatcggg gaccgggcgg gattcctggc atcagcctca tacggaggaa
     1441 tcgacatcag ctccggtccc tatgacgtgg ttctctcgcc ggttgcgttc gcccagctga
     1501 ttggtgcagt gctgctcccg gcgctttcag gccggaacgt ccacgccggg cgatcgaagc
     1561 ttgcaacttc actcggagac caggtcatcg atccggccct ctccctctcc gatgatccat
     1621 tcctcgaaaa aggacttgga agcaccaggt gggatgctga aggggttccc acccggcatc
     1681 tctcgttcat ccggaaggga gtccttgaac acttctccta tgacctcaag acggcctccc
     1741 ggtacggtaa ggagagtaca gggagtgcag tccggaccgg tcatgggggc gggcctgcca
     1801 tcgggaacca taatgtcatc atcgatggcc cccggatgga cgtcatggag gacgaggcga
     1861 tctatatcca ggacgtggtc ggagcccata cggcaaaccc gctctcaggc gacttctcag
     1921 tcgagctcac caacccgttc cgcatttccg gcggaaccta tgaaaaacca gttcgaaaag
     1981 cgatgatgtc cgggaatgtc tttgacatgc tcggggatac ttccggctta ggccgtgatg
     2041 acagagttat cgggtcaatc gtagtgccgt caataagatt aaagaaacag catatcattg
     2101 gtacgtaaaa tgtcgttcga tcttgccctg atcgttgccg gaatattgat cgcggtcgta
     2161 atgatttttc tgttgaagga tatcgtaaaa ataatcatca attccatcct tggcctcctt
     2221 cttctcttct tcatcaatta ctttgacctt ctcgggtatg ttggaaggcc tgacatcccc
     2281 tataccccgg taaatatcct cctttgtgca cttggaggga tcccgggggc gctcatcgtc
     2341 attatcctcc agctgacggg aatcaccatc acctgattcc tcatacggct cattccatga
     2401 ttgctggcac tccttctcag tgggtgtgta ctccttcagg ggccattact ctcctttgga
     2461 taatctgcct gatgagaggc agttattatg tcactcccac atcccacgta tacacagaat
     2521 gaatcaggaa gggatccatg gaagaggctg agtactgtct ggatgaccgc agaaccctcc
     2581 tgctgctcaa gggcccatca tcgtttatcc ttgccgggaa ggctggatcg cggtttcccc
     2641 tttgtgtcga atatggagac ggtgagatct gcaccaccat cgagaaaacg gatatcattg
     2701 cggtatctgc ccccgaagga ggtgctctcg agcccgcggt gatgctgatg gagcttgtca
     2761 gggcgcacca tgttcccctc ctcgtccttc cccaggggca tcccggatcg aaacggctca
     2821 gatatgtggt ctctgccggt cctgagatct cactgtcctg tggcatccag cgggggaccc
     2881 atccggatca gcacctgctc tgttcatctg gcgaactggc cggcatcctc ctgtccggga
     2941 cgatggaagg gatcagggta gcttcgatgc catcatccgt caccgccctg atcctcactc
     3001 attcactgac gattggcacg aaggccctgc gaggagggag ctgaccgtga acaggtaccc
     3061 ggtttgattg gcaggttttc cccttcatat ccttcaaggg ggggagaacc gatcgatggt
     3121 ttccgcgcat accaacaggc ctataccggg gttccagcaa aaaaataccg gggctccaat
     3181 aaaaaaaata tttccaggaa tcaattataa ttgccatatt tcgataatca gcgctttttc
     3241 cggcgggaaa aatatcaaca gatataagaa caaatctgtc aaatatctca gctgccgtga
     3301 gaggaggttg gatgaaaact gaggtattaa agcacatcaa agcaaccgaa gacgagtacc
     3361 agtcgttgat aaacacggtt atggctgaga ggaaacgcat acttgctgac gctgaactcg
     3421 aggctgacaa cctgattatg aaagcaagca ccagcgccga agagtacaga aagatgcgac
     3481 ttgccgaagc acgggcgcag gcagaacgga agcatgccga gattgtcagg gaaggcgagc
     3541 ggaaatctgc tgatctgaag gaaaagggca cacaaaacct taattcggcc gttgaactgc
     3601 tggtttcccg atttaaggag agcctgcatg ctcacccctg aacagatgag caaggtcctt
     3661 atcgcgggcc cgaaggatgc gctggatccc gttatcgcgg agcttcatcg ccaccggctt
     3721 tttcacatcg aagactttgt ggagaatgac caggaagagt ataccgggta tcggataggg
     3781 aattccctgg aaggtgccgg agatatctcc aaggaactgc tcagactgag gtcggttacc
     3841 ggggcattct ccctgcgggc agatgacctg gatcccaagg cacggatcag gagatcggaa
     3901 ctgggtgcag cgatcgaaaa ggagcttccc cggatcgaag acgaggtgga gtcgctcctc
     3961 accaggaaga cagcgatcga gaaccatatg aaggagaccg agcagaagat cgaggcgctg
     4021 acccccttcg cagatttgag aaccgatctc tccctgcttc acgggtaccg gacgcttacc
     4081 ttctttgccg gcaccatcgc aaagaagatt gacctcgggg ttccgagcga agtatactat
     4141 tccgaatcca agaaaggcaa tttccttgtc gcagcagttc ctgccgagta ccgtcaggaa
     4201 gtggagagaa aactgctcga tgcccagttc caggctgttc cgattcccga tgagcagggg
     4261 cctgccaggg aacggatcag cgtgtacaat gtgcagctgg aaaccgacaa gaaggatatc
     4321 gaagagatag cgagccagat caacgctctg aaatcccagc actccgagtt ccttgtagcc
     4381 tgtgacgagc tcctttccat cgaggcagaa aaagcggagg tcccgctccg gtttgcaaca
     4441 acagaccatg cgtttgtagc ggaaggatgg gtcccgaccg aatcgcttga aggccttgta
     4501 aaggggatca caactgcatc ggggggcaag gcatatgtca cgcagctgcc ggtcgaggag
     4561 ggaaaggatc tcccgcccat ggagtacaat aacgtcaatt ttgtgaagcc aagccagttc
     4621 ctgatcgatg tgtattcccg gccgaagtac aacgagctcg atcccaccct cattgtctcg
     4681 atcatattcc ccatcttctt tgggttgatc ctcggcgatg tcgggtacgg tgcaatactg
     4741 cttgcgatga gccttggtct caggatggtc atcaagggag atgacggacg gatgctcctt
     4801 gatgtccttc ggaatgcaag tatttcgagt atcatctttg gcttcctgta cagtgaattc
     4861 ctgggattcc cgatccccgg cctgcacccc ctgctcccga caagacacct gaacatcggc
     4921 ggagaggcag gcggtcatgg gccggcggtc cctgagctga tgataatggc catctggatc
     4981 ggcattgcat acatcaccct cggccggatt cttggcatga tcaaccatgc caggcaggat
     5041 catggatctc accgggtgaa agcggtcctg gcaaaccttg gctggattat ggtgatgtgg
     5101 ggtatcctgc tgatgatctg gtcattcttc gccatgccgc tgatgccaga tctcaccgga
     5161 tatcccccgc tggttgcagg actgagcatg ccagcccttg caggggcggt gatgatcctt
     5221 gtcggcattc tcttcattgc aagggactcg gcactggaaa tcgttgaact gcccactatc
     5281 ctttcccacg tgctctcttt tgcacgtctg gttggtgtgg gtctctcgtc agttgctatc
     5341 gccatggtgg tgaactttat cgccatcggg cttattattg aaccgcagtt ggaacgtctc
     5401 accatcattg gtgtaataat catcatagtg ggggtttttg tattcctgct cgggcacctc
     5461 ttcaatgcta tcctggggct gctcggggga ggattgcagt ccctgaggtt gaattacgtc
     5521 gaattcttca ccaaatttta caaaggtgga ggaaagaagt acaacccgtt tggattgaaa
     5581 agaagattta cggaggacta aaacatggtt ggagaagaac ttgtcggatt gacacctgag
     5641 atgatcgagg cctcacaggc cggaatgaag gcacttggtg caggtcttgc tgtcggcctt
     5701 accgggattg gaacaggact ggccgagatg gctatcgggt ccgcagcagt tggagcgact
     5761 gccgagaaca aggacgtttt cggtcttgtc ctgttactga ccgttattcc tgaaactatc
     5821 gtcatctttg gtcttgtggt cgggcttctg ctgttattct aaacgtagga gcagaccatg
     5881 ggactggaag tggtcatcga cgatatcagg gagaaaggga agagagaagg agaccggatc
     5941 aaaaaggaga ccgatgctga agtcgggagc atcctgatga cggcacagga gaaagcagag
     6001 cggatcaaac tggccgccga gcaggatgtc cagaaacagg tggagcatat cacgaaccag
     6061 gaggcatcag cggcaaacct ggtagtgaag cgccagcttc tgaacaccca gaaggagctc
     6121 ctcgatcagg tctattcagc tgccctttcc cggatagcag cgctcccgga agatttccac
     6181 agggatacca taaagacgct cctttcgaga gcgagagcag aggtccctga gggcattgtc
     6241 cactgcagca ggcgggacag tgagctgctg agccggattc tctcatcgga tacctcattg
     6301 aaagggtata ccaagggaga tccggtggag attgaaggag gcgtcatcat cgagagtaag
     6361 gacggagagc tccagcttga ctacagctac cggacgttcc tgaacggcgt atgggagaca
     6421 gggttgaagg atgcctcggg aatcctgttc cgttgaggtg atgcaggatg gctgacatca
     6481 gtgccggtcc tgccccctat gtctatgtct gtacgaggct ccgggtaagg aaagcaaagc
     6541 tcatcccaag ggaggattac ctccggctgc tcaatatgag catccccgag atcacccgtt
     6601 tcatcgagga gacgcagtac aagagggaga tcgatgagct tgcctcatcg ttccatggga
     6661 ttgacctcat cgaacttgcg ttatcctgga acctggcaaa ggaataccag agcatccagg
     6721 atattacgcc cggtgttctg aaacgattca cgcagtccta tctccggaag tgggacatcc
     6781 agaatatcct taccatcctg cggggaaaga cccaggggat gaaaccgggg aagatccgtg
     6841 agatcctcgt acctgccggg gagatggaca gggtttttct ggaccggctc gtaaaagaag
     6901 agacaccgga acggatcctc gaagcactca aaggccagtc ggtgtatcct gccattgctg
     6961 ccgggttttc aacggcggtc tctgaaggat cgttcgccag gattgagaat gaactgtaca
     7021 agcagtttta tgaaaacctg ctgtctgagg cgacgagcgg cttcaagggg ggcaagcagt
     7081 tcctcgagta catcatgctg gacattgata ttaccaatat caggaacatg ttcaggctca
     7141 gggctgacac gcttcacgaa gatgcacgtt cgatgatgat cccgggaggg acgttttcga
     7201 ccgatgcatt ccagcatctc atcagtatcg aggaatcaaa ggagtttatc gataccctga
     7261 aatctaaggt acatgtgaag ccattgatca tgctcctcga agagatgagc ggcggggaga
     7321 agtcactccg tgagattgag atcgacctga tcaaggtcca gcttgcccag atggagtcat
     7381 tatcgaaact caatccgttc tcgattcacc ccatcctcac ctaccttgag aagaagaagt
     7441 acgagatctt caatctccgg gctattgccc ggggaaagga agtgaacctt ccgaccgacc
     7501 ggatacggag ttacctggtg atgtgagatg gagatagcag ttgttggcag cagcgatttc
     7561 gtcctcgggt tccggctttc agggatcaga aggacctatg cagcagaatc cgacgagaag
     7621 ctcgctgaat acatcacgcg gattctggat gacgggacca tcggtatcct tgtcctccag
     7681 agcagggata tgaacaggat cccccggcgt ctccagacca cgctcgagaa ttcagtaaaa
     7741 ccgacagtta tcgcaatcgg tggcgaggag ggaggtatgt ccatgaggga gagaataaag
     7801 cgatcagtgg gtgttgatct gtggaagtaa cagagaagaa agagaaaggc caggaaggag
     7861 tcctcaaacg gattgccggc cctgtcgtca ctgcggtcaa tattgatgcg cacatgtatg
     7921 atgtggtgaa ggtcggccat gaggagctca tgggagaggt gatcaagatc cagggtgatg
     7981 agaccatcat ccaggtctat gaggatactt cagggatcag acccggagaa cccgtgacga
     8041 acacgggact ttctctcgcc gtggagctcg gtccgggcct cctgaccagt atttatgatg
     8101 ggattcagag acccctgtcc gtgctggtgg acaagatggg agatttcatc cagcgagggg
     8161 tctctgcacc agggcttgac caccagaaga aatgggagtt caggcctctt gtaaaggaag
     8221 gagacaaggt atcccccgga atggtcatcg gggaggttca ggaaacaaac atcgttcacc
     8281 ggatactcgt acccccaaag gtccagggtg gagtggtaaa gagtataaaa cggggttctt
     8341 tcacgataga agaggtggtg tgcaccctcg attcgggaac tgaactgacg atgatgcaga
     8401 aatggccggt ccgtgtgcca cgcccggtaa ctgagaagat gaacccgacg atcccgctca
     8461 tcaccggcca gcggatcctg gacggcctgt tccccatagc aaagggagga accgcagcga
     8521 tccccgggcc tttcggcagc ggaaagaccg tgacacagca gcagcttgcc aagtggtctg
     8581 atgccgagat cgtggtgtac atcgggtgcg gggagcgggg caacgagatg accgaggtgc
     8641 tgacagaatt cccagagctc gaggacccga aatcaggaaa accgctcatg gaacggacgg
     8701 tgctcatcgc aaacacgagc aacatgcccg tcgctgcccg tgaagcatcg gtgtataccg
     8761 gtatcacgat tgcggagtac ttcagggaca tgggctacga tgtctcgctg atggcggatt
     8821 cgacatcccg atgggcagag gcaatgagag agatctcgtc acgactcgag gagatgcccg
     8881 gggaagaggg gtaccctgcc taccttgcag cccgcctttc cgagttctac gagagggccg
     8941 ggcttgtcag gacgctttcc gggaagaacg gatcggtatc cgttatcgga gccgtctccc
     9001 cacctggcgg ggacttttca gagccggtca cccagaatac gctgcgtatc gtgaaggtat
     9061 tctgggctct cgatgcaaag ctctcgcagc ggcgccattt cccggcgatc aactggctca
     9121 actcctactc gctctacctt gattccctcc atgactggta cgacaaggaa gtatcacccg
     9181 agtggaatgt gctcaggtct tgggccatgg aagtcctcca gaaagaggca gaactccagg
     9241 agattgtcca gctggtcgga tccgatgccc tgcccgaggc agaacagctg accatcgaga
     9301 ttgccaggat gatccgggag atattcctcc agcagaacgc ttacgatgcg gtcgatacct
     9361 tctgtgatat gtccaagcag tacgacatga tgaaggcgat caaggggtat gcagaccttg
     9421 catatgcagc ccagactgcc ggggtatccc ccgcacagat catcacgatc aagtccaaga
     9481 acgacctgcc ccagatcaag ttcatcaagg attacaagcc tgaactggaa cggatccaga
     9541 agagcatgga cagcgagatc aacaccctga ggtcgacagc atgaaagagt acagaacgat
     9601 ttcgaagatt gcaggtcccc tggtgttcgt ggagaagacc gaaccggtcg ggtacaacga
     9661 gctcgtcaat atcgccctct gggatggtac catcaagaga ggccaggtgc tcgataccag
     9721 cgattccctc gtcgtcgtgc aggtctttga gaccacggca ggaatcggga aggactcggg
     9781 tgtccggttc ctcggcgaga ccatcaagat gccggtcgga aaggagatgc tcggccgtat
     9841 cctttcaggc ggaggaaaac caattgacgg ggggccggag attgtccccg agaagcgact
     9901 ggatatcacc ggcgctgcca tcaacccgta tgccagagca tcccccgagg agttcatcca
     9961 gacagggatc tccaccatcg acgggaccaa caccctcgtg aggggtcaga agctcccgat
    10021 cttctcgggt gcagggctcc cccacaacaa catcgcactc cagattgccc ggcagtcaaa
    10081 ggtgctcggc tcgaaggagt cgtttgccgt ggtctttgca gccatgggta tcacgaagga
    10141 agaggccaac catttcatgc aggacttcga gaggaccggt gcccttgaac gggctgttgt
    10201 cttcctcaac ctcgcggatg acccggctgt ggagcgtatc atcactccga gacttgccct
    10261 gaccaccgca gaatacctcg ccttcgacct ggatatgcac gtgctcgtga tcctgaccga
    10321 catgaccaat tactgcgaag cgctccgcca gattggggct gcccgtgaag aagtgccagg
    10381 acggcgtgga taccccggat acatgtatac ggatctcgca gggatctatg aacgggcagg
    10441 gatcatcaag ggcaaaaaag gttcgatcac gcagttcccc atcctcacga tgccgggcga
    10501 tgatatcacg cacccgatcc cggaccttac cggatatatt acggaaggcc agatcgtggt
    10561 ctcgagagag ctgcaccgga aaggcatcta tccccccatc aacgtccttc cttccctctc
    10621 ccgtctgatg aacctcggta tcggaaaaga gcatacccgc gaagaccata aaaaggtctc
    10681 cgaccagctc tatgccgcgt atgcagaagg caatgatctc aggggcctgg ttgccatcgt
    10741 cggaaaggat gcgttatccg aacgcgaccg gatgtttttg gagtttgccg atctgttcga
    10801 gaaccggttt gtccgccagg gatacgatga ggatcggacg atctttgaga cactcgatgg
    10861 cggatggaac ctccttgcaa cgctccctgt cgagcagctg gtccggatcg atcgcgactt
    10921 gatcaacaag taccacccga agttcaggca gggtgccaag gcgcaggagt aacggcacat
    10981 ggcactgcgc gatatcaagc ccacacggtc agaactgatc aacctgaaac ggaggatcgt
    11041 gctgtccgag cgtggctaca agatcctcaa gatgaagcgc gatggcctga ttattgaatt
    11101 tttcaaggtg cttgagaagg caaaggacag ccagagcgac ctccagcaga agtacgaacg
    11161 tgccacccag atgatggcgt ttgcaaacac cgtggaggga gccatagggg taaaatccgc
    11221 agc
//
LOCUS       DAOOCO010000047        11067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5523_length_11067_cov_6.109244, whole genome shotgun sequence.
ACCESSION   DAOOCO010000047 DAOOCO010000000
VERSION     DAOOCO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_11067_cov_6.109244"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK154_04055"
     CDS             complement(<1..202)
                     /locus_tag="PK154_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HPW10269.1"
                     /translation="MGWLSRIFGTKDSSKKPEERPVPVKADVEKKAEVLQKEDKPVAD
                     KIKVGHVHMSGCTGCLVSLADNY"
     gene            complement(123..662)
                     /gene="frhD"
                     /locus_tag="PK154_04060"
     CDS             complement(123..662)
                     /gene="frhD"
                     /locus_tag="PK154_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HPW10270.1"
                     /translation="MLFPEIVVAGCGNPLFADDGFGPAVAEELGRLEIPDTIKVIDAG
                     LGGPHFIFSLIDPEHTKRLIIIDIADFGAEPGEIRDFSVEDLPAGSYRDIHSWDLTEP
                     LQRLKDTIDITVIGCQPRRVTAPEFELGLTDEVQKAIPRTVRHVLELIGVDYGMAIKN
                     LRNEGQQQKTGRETCSGES"
     gene            complement(673..2039)
                     /gene="frhA"
                     /locus_tag="PK154_04065"
                     /pseudo
     CDS             complement(673..2039)
                     /gene="frhA"
                     /locus_tag="PK154_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
     gene            complement(2336..3283)
                     /gene="mptA"
                     /locus_tag="PK154_04070"
     CDS             complement(2336..3283)
                     /gene="mptA"
                     /locus_tag="PK154_04070"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HPW10271.1"
                     /translation="MGRDLPDIQSTTPAVRINLTRVGVKNVKKLVEVSRGEKRPVIFI
                     SNFDVFVDLPGSLKGANLSRNFEVIDEVLQQAIDGDVKEIEDLCSVVARKLLDRHEYA
                     DRTEVLMRSQFMVKRETPVSHTSCHEVVKVHARAVARRTFRDPIVRKSIGAEVTGMTA
                     CPCAQDIMKERAVYVLKDLKIEDEKIDAFLREVPMATHNQRGRGFLCIEIDDDQHVKL
                     EKIIKILKDSMSTNIFELLKRGDESYVVLNAHKNPRFVEDCVREVAKKVLAEFRDLPG
                     DSLITIKQTNEESIHQHDAYAERRATMAELYVELNGEQK"
     gene            3408..4400
                     /gene="ilvC"
                     /locus_tag="PK154_04075"
     CDS             3408..4400
                     /gene="ilvC"
                     /locus_tag="PK154_04075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW10272.1"
                     /translation="MIKKFYESDADLSVFDGKRIAVIGYGSQGRGQALNLKDSGLDVI
                     IGLRQGKSWDLAKADGFKVYPVSEAVKTAQVVQILLPDENQGGVYKNEIKPHMTTGKC
                     LMFSHGFNIHFGQIVPPPDVDVIMVAPKGPGALVRRMYEEGKGVPALIAVHQNYSGHA
                     QEIALGYAKGIGATRAVVLETSFREETETDLFGEQAVLCGGMTSLIKAGFETLVEAGY
                     APEMAYLEVLHETKLIVDLIYEGGFTKMRNSISNTAQYGDLTRGPRVIGPESYLAMQE
                     ILREIQTGEFAREWMLENMVNRPVFNALTKEDEEHLIEEVGKEVRSLMPQFQKK"
     gene            complement(4696..6774)
                     /gene="purL"
                     /locus_tag="PK154_04080"
     CDS             complement(4696..6774)
                     /gene="purL"
                     /locus_tag="PK154_04080"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPW10273.1"
                     /translation="MLSAADLSTIRTTLRREPTDVELACFENLWSEHCSYRSTKRLLK
                     TLPTSGADVILGPGDDAAIVRFSERTALAISMESHNHPSYVDPHDGAATGVGGIVRDV
                     LSMGARPVALMDPLYFGPLDREKNRYLLEHVVEGIGGYGNCIGVPVVRGDLALDSSYN
                     GNPLVNVVCVGIVHPERFITARVKQPGNHLVLIGASTGRDGLGGASFASRDLSEDSEA
                     EDRPSVQIGDPFTEKLLIEAILEMAETGKIFSCRDLGAAGLAGASSEMCSTFGGLIHA
                     DRVHQREPNMRPVEIMLAESQERMLLEVAPSDASLMGSIAEKYDLKWSDIGQVIAEPR
                     YIVKFHGKTVADIPILFLCSDAPECNWESQPYNEVKPFTPPPGTLDDLLLKVLSYPEV
                     AHRTWVVEQYDHDVQVRTISLFHDAALLRLGGESLGLAFSCGCNPRQIFLSPYSGTAN
                     AVYENAANLACIGADPLCIVNCLNFASPVHTDIYWQLSECVKGMGDMARSIGVPVVGG
                     NVSLYNESDEMLTRIKPTPSIGMVGRVPVTRAVKPEPGMGLAVCGQEGAHFGGSVLDA
                     ILSCGGTPPPKAEHSVLKAIRSLSASDRNIAVTDISQGGLAAALATFLPGARVRLDGP
                     ALPTLLSETYGRFLIAYKSESSLEDLDCRIIGEVTSEGLEIRHPGGICSLSKDQLEFA
                     LSSFTRIMKG"
     gene            6871..7482
                     /locus_tag="PK154_04085"
     CDS             6871..7482
                     /locus_tag="PK154_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HPW10274.1"
                     /translation="MVDPYEALLKKAYSNVTEQSESTERFTVPDARVYIEGKTTILEN
                     FAEIADVVRRDQDHLMKYLLGELGTAGKIDGTRAIFNGKFESSLINGLVKGYVDDYVI
                     CSECGKPDTRLVKDDRVLLLRCDACGGHRPVRKRKARSEPVGSDLQEGQELDVEVQSV
                     SKRGDGVVRMGRYIMYIPRGKPGQKVKIRISRISGSIVFTEQI"
     gene            complement(7500..7769)
                     /locus_tag="PK154_04090"
     CDS             complement(7500..7769)
                     /locus_tag="PK154_04090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HPW10275.1"
                     /translation="MNIKILELEKNKARLIISGEGHTFMNTLTEEILTDPGVDVARYL
                     IKFQFSDPELLVTTKGDRDPLAAIKDACTRLSGYCTELLGKIPEK"
     gene            complement(7907..8626)
                     /locus_tag="PK154_04095"
     CDS             complement(7907..8626)
                     /locus_tag="PK154_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW10276.1"
                     /translation="MNITVLGTGDAIGTPKVGCSCPQCTYALTHGTHRLRTSFLVEHN
                     GHTILIDSSPDLRQQLLVYGSPHIDAVIWTHGHYDHFMGFGEFYRVQSMPAVYAAPQV
                     LDYCGNIFRFLKFQRNPTEPFRPFSLFGLRVTLVPVNHPPVETYGLVLTDGKAKIAFT
                     SDSNADIPPETLSVFSKADLLFVDALVPPSIQIPKHMNYNEACALAKRLMVGDFRCVH
                     MSHTIPWDLPHTGKDGETFCF"
     gene            complement(8628..9902)
                     /locus_tag="PK154_04100"
     CDS             complement(8628..9902)
                     /locus_tag="PK154_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW10277.1"
                     /translation="MSQEDETLKLLEVALTAEIFNQHQNLDINDLTPECRILFGVDGN
                     GEVKRPLSVSEGMIKRSLGIQDACSKLSKIPVITCDDFGQRIHVTSLDAAVRWFVRKG
                     GINLAGRNPALAYYLGSLDTTAPSYPLVRAANPPYEDTRLFLDKKIATILSEDESLKS
                     GMDLVIISAPDEVEQTFENFVCTDGQKRIISKIKTAREHRETLQRHGIHEVGKLLFVG
                     PPGTGKTSLALGLSREMHLPLIEVRLSMVTSQYLGETSKNIDRIFDFAKRLAPCILFI
                     DEFDFVAKSRVTDDHGAMKRAVNSLLKNIDRISLIRNGVLLIGATNHPQLLDEAAWRR
                     FDEIVEFSLPEREMRAQIIRNITGSIDCQCDYGTVADNTEGFSGADLRIMIKEALLSA
                     LIEGRSHITDCDIEQGIILVSNRGAVRRQNWL"
     gene            complement(9947..10828)
                     /gene="map"
                     /locus_tag="PK154_04105"
     CDS             complement(9947..10828)
                     /gene="map"
                     /locus_tag="PK154_04105"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HPW10278.1"
                     /translation="MIPDDDSLFCYSEAGKIASVILKKGSSLIEIGNRVLETVESIEQ
                     MVEEQGCGLAFPLNLSFNEDAAHDTASADEQRVFSEGDVVKLDLGVHLEGYIADTAVT
                     VDLGDNALLIEASKAALTKAIEIVRPGVTSGDLGRAIQHEIQVRGYHPVSNLTGHGLA
                     RYSIHTPPSIPNISFQGGVVLEEGMVFAIEPFASTGSGRVSEKSRIEIFQQTAVKPVR
                     LPSARKLLESVRNRHGMPFARRWITDGKKDIALASLISQGIVRSYPVLSDVPGSLVSQ
                     HEHTVIVTSDGCIVTTA"
     gene            complement(10831..>11067)
                     /locus_tag="PK154_04110"
     CDS             complement(10831..>11067)
                     /locus_tag="PK154_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPW10279.1"
                     /translation="LQPHKIGSQLGLGHGVGLEVHELPSLGPGGDRLSRGHVVTNEPG
                     LYYEKVGGIRLENIGLVTQTGFRTITKFPCELVI"
BASE COUNT         2662 a         2863 c         2803 g         2739 t
ORIGIN      
        1 cgtaattgtc tgctaaggat acaaggcagc ctgtacatcc actcatgtgt acatggccaa
       61 cttttatctt gtctgccaca ggcttatcct ccttttgtaa aacttctgcc tttttttcaa
      121 catcagcttt caccggaaca ggtctctctt ccggtttttt gctgctgtcc ttcgttccga
      181 agattcttga tagccatccc atagtccacc cctattaact ccaatacatg acggacagtt
      241 cttgggattg ccttctgtac ttcatcagtg agccccaatt cgaactcagg ggcagtaacc
      301 ctccgtggct ggcatccgat aaccgtgata tcgatggtat ctttaaggcg ctggagcggc
      361 tcggtgagat cccacgaatg gatatcccgg tagctccccg caggcagatc ctcgaccgaa
      421 aagtccctta tctcacccgg ttctgcacca aaatcggcaa tatcgatgat gatgagccgt
      481 tttgtatgct cgggatcaat cagggaaaaa ataaaatgag gtccgccgag tccggcgtca
      541 atgaccttta ttgtatcggg gatttcgagg cggcctaatt cttctgctac ggcggggcca
      601 aacccgtcat cagcaaagag cggattcccg caccctgcaa ccacaatttc cggaaacagc
      661 atggggttac actcactgga tgagtttctg ggcaactatc ctcttgtctt catcaatgac
      721 aatcatgtgg gtagcgcatg agacgcacgg atcataaccg cgtaccacca tctcggcaac
      781 ctgccaaggc gcacctgcca gtgcacggct gcaggtcggg aagttccagg tggtcggaac
      841 gagcatctcg taccagagca cacggccatc cttgaccttt gcaaggtgga catcagtgcc
      901 acggggggcc tcattcgctg cccagcccat agaaccatct ccctggggaa tgggatctgc
      961 aaggaccttt cctgagggat tgtactcatc aagggcattg atgatagtat agagacagtc
     1021 ggtgtactcg agctggcgtg cgacatgctg gccccaggtt cccttctcgt catagttttt
     1081 gaacttgacc agtcttgccc gtggccctac ctcaacaggg gcaccgtcat ataaggggac
     1141 tccggtgcag gcttccatct gggggttgac tttcgtgccg acaggggaag taccgccaat
     1201 cgggtagctc ggatctgcca ggctgacttc tccctcaccc atgtaccagt cccatggcct
     1261 gacatcagtc cagcgccgtg gatcccagcc ggggttctcg tcgaggtttg agctgccata
     1321 catcggatcg gctgccatgt agccctggtt gtggtatccg agggtctttg gcagcgggat
     1381 ctgttttccg ccgacctctg tccagtctcg cttctgcata ttcctgatca cggcaatcat
     1441 gaaatccatc tgctcgtggg caagaaccag gccttccttt gcaagatcgt acatcttgct
     1501 cttcgcatgc ggggtgcagg ccttgtacat tccaccgaca cggggattac tcgggtggac
     1561 tgcctcacca ccggcgattt cgccgatcgt ctggcctatc tccctgagtc tctggatccg
     1621 ttttgcgaca ctccggactg gttcttctgg ggtgaacgga ttgatcttcg tatccgttcc
     1681 cgggatatag aagtcgggaa gaatcaggat attgtgcagc gcagtgctgt ggagccggtt
     1741 gggacactga gcacgatacg gaggagcttg gcatccctcg ggatctcgca ccggatagaa
     1801 ccttccatcg actcgattcc ggcaagcgta tgggcaatcg gacagatacc acagacgcgt
     1861 gatgcgatct ttggcacctg gtccatggtc ttgccaatgg caagcttctc cacaccccgt
     1921 acaggggtga tggagagcca gtcaccacgc tcaatgatgc ccgcatcgtc cactttgagg
     1981 acgagctttg agtgcccctc atgccttgtg gttggggata tctctacaat tttcgacaat
     2041 agtatcgcct catattattg agttgttcgt ttttgcggta ctggtgttaa tccacacaac
     2101 tcatctcaat ggatgaatga ccgtacagag tacgttcatt tacaatagat tcactcctgt
     2161 atatatcagc tgtgtcttat tccagtccga aatggccttc aaatgccaaa atagttaatt
     2221 ttatatggaa attatattgc tgaaatggta atcagcagcg gaaggggatt tttgaaaatt
     2281 cacaacccca ccgcgtcaga aaaaattgtt gatggcacct atcgccagag ttgattcatt
     2341 tctgttcgcc attaagttcg acgtaaagct ctgccatagt agccctgcgc tccgcgtagg
     2401 catcatgctg gtggatactc tcttcgtttg tctgcttgat ggtaatgaga gaatcgccag
     2461 gaagatccct gaattcagca agcactttct ttgccacttc tcttacacag tcctcgacaa
     2521 accggggatt cttatgggcg ttcagaacga catagctctc atcaccccgt ttgagtaatt
     2581 caaagatatt ggtgctcatg gagtccttca gaatcttgat gatcttttcc agttttacgt
     2641 gctgatcatc atctatttcg atacagagga atccccggcc acgctggtta tgggttgcca
     2701 tgggaacttc gcgaaggaat gcatctatct tttcatcctc gatttttaag tctttcagga
     2761 cgtagaccgc ccgctctttc atgatatcct gtgcacaagg gcatgccgtc attccggtca
     2821 cttcagcacc gatactcttc cgaacgattg gatcacggaa tgtccgcctg gcaactgcac
     2881 gggcatggac cttcaccact tcgtggcaac tggtatggga taccggagtc tcccgcttga
     2941 ccatgaactg gcttctcatc agaacttccg tccggtctgc atattcatga cgatccagca
     3001 gtttgcgtgc caccacgctg cagaggtctt cgatctcctt aacatcacca tcgattgcct
     3061 gctgaagaac ttcgtcaata acttcaaaat tcctcgaaag gttcgctcct ttcagacttc
     3121 ccggaagatc aacaaaaaca tcgaaattgg aaatgaatat gaccgggcgt ttctcacccc
     3181 gggaaacctc aaccagtttc ttgacattct tcacgccaac acgggtgagg ttgatacgga
     3241 ctgccggggt tgttgactgg atgtctggca aatctcgacc catctttgca ttctttagtt
     3301 cgtgaatatt gcatcgctcc cgctcattgt attttggaca gggtgtgaaa tgtaattttt
     3361 ttatataata tttaagtgct ctgttagcga tatctcttgg acgtagtatg atcaagaaat
     3421 tttatgaatc cgatgctgat ctttcggttt tcgatgggaa acgtatcgca gttatcggtt
     3481 acgggtcgca gggaagagga caggcactga acctgaagga tagtggcctc gacgtcatca
     3541 tagggctccg acagggaaag agctgggacc ttgccaaggc cgatggtttc aaggtgtatc
     3601 cggtgtctga agcggtgaaa acagcacagg tcgtccagat actgctcccc gatgaaaacc
     3661 aggggggagt atacaaaaat gagataaagc cccatatgac gaccggcaaa tgccttatgt
     3721 tctcccatgg attcaatatt catttcggac agatcgttcc tcctcccgat gtagatgtta
     3781 tcatggtggc accaaaaggg cccggtgctc tggtacggag gatgtacgag gaaggaaaag
     3841 gagtcccggc acttattgcg gttcatcaga actattcagg tcatgcccag gaaattgccc
     3901 ttggatatgc gaaagggatt ggcgcaaccc gcgcagtagt actggaaacg agcttccgtg
     3961 aagagactga gacggacctg ttcggagaac aggcggtact ctgcggcgga atgacctccc
     4021 ttatcaaggc cgggtttgag acccttgtcg aggctggcta tgcacctgag atggcgtatc
     4081 tggaggtact ccacgagacc aagctgattg ttgatctcat ttacgaagga ggctttacga
     4141 agatgcgtaa ttcgatctca aatactgcac aatatggaga tctcacccgt ggtccacggg
     4201 ttattggccc agaaagctac cttgccatgc aggagatcct gcgtgaaatc cagacagggg
     4261 agttcgcccg cgaatggatg cttgaaaaca tggtcaacag accggtcttc aacgcgctca
     4321 caaaagagga cgaagagcac ctcattgaag aggtgggaaa ggaagtccgc tccctcatgc
     4381 cccagtttca gaagaagtaa tccaaatcct cccgttcact ttttgagata tttttggcgg
     4441 aaccttattt caagggagta tgcccacgca aaacaaccct ccagctcatt ccctgcatta
     4501 gtcttgtttc caaaatccag agagtatgaa aggatcatcg ccctcctgat aggttctata
     4561 cattcaggga ctcggtataa gggaactcta tggggaaatc ctattcacga tgggttaata
     4621 tcggtgtaaa agggcattcc cccggcagtt tgggaattgg gccctttcct tctccgattc
     4681 atctcgccaa aagatctaac ccttcataat acgggtaaag gatgaaagag caaactcgag
     4741 ctgatctttt gagaggctgc agattccccc gggatgccgg atttcgagtc cttctgaggt
     4801 aacctccccg attattctgc agtcgagatc ctccagtgag gattcgcttt tatacgctat
     4861 cagaaacctt ccatatgtct ctgaaaggag tgtgggaagt gcaggaccgt caagtctcac
     4921 ccgcgctcct ggcaaaaacg ttgcaagtgc ggcagcgaga cccccctgag agatgtcagt
     4981 tactgcaata ttcctgtcag aagcggacaa tgagcggatg gccttcagaa cagaatgttc
     5041 agcttttgga ggcggcgttc cgccgcagga aaggatcgca tcgagaaccg atcctccaaa
     5101 atgggctccc tcctgcccgc agactgccag ccccataccg ggttcaggtt tcaccgcacg
     5161 ggtaacaggg acccggccaa ccattccaat cgacggggtt ggttttatcc gggttaacat
     5221 ctcatcgctc tcgttgtaga gggagacatt tcctcctact acagggaccc cgatactgcg
     5281 ggccatatct cccatccctt tcacacattc agagagctgc cagtagatat cggtatggac
     5341 cggactcgca aagttcaggc agttcacgat acagagcgga tcagccccga tacaggcaag
     5401 gtttgccgca ttctcgtata ccgcatttgc agtaccgcta tagggggaga ggaatatctg
     5461 tcgcgggttg catccgcagg agaaagcaag accaagggat tctcctccga gacgtaacag
     5521 agctgcatcg tggaaaagag agatcgtcct tacctgaaca tcatgatcat attgttcgac
     5581 cacccaggtc cggtgtgcta cttccggata ggagagaacc ttgaggagga gatcgtccaa
     5641 cgttccagga gggggggtaa atggtttcac ttcgttatag ggctggctct cccagttaca
     5701 ttccggggca tcgctgcaga ggaagaggat cgggatgtca gcgaccgttt tcccatggaa
     5761 tttcacgata taacggggtt cagcgatcac ctgcccgata tcgctccact tgagatcgta
     5821 tttctcggca atgctgccca tcagggatgc atccgatggc gccacctcaa gaagcatacg
     5881 ttcctgcgat tcggcaagca tgatctcaac cggacgcata ttgggttcgc gctggtgtac
     5941 acgatcagca tggatcagac cgccaaatgt gctgcacatc tcgctggaag ccccggcaag
     6001 cccggcggct ccgagatccc ggcaggaaaa gatcttcccc gtctctgcca tctcgaggat
     6061 ggcctcaatg agcagctttt ccgtgaacgg atcccctatc tggacactcg ggcggtcctc
     6121 ggcttctgaa tcttcagaca aatccctgga ggcaaatgac gctcctccaa gaccatccct
     6181 ccctgtcgat gcgccgatga gaacgaggtg atttcccggc tgttttaccc gcgcagtgat
     6241 gaaacgttcg ggatggacaa tacccacgca gaccacattc acgagtggat tgccgttgta
     6301 actgctgtcg agcgcgagat ctccccgcac aacaggaacc ccgatacaat tcccgtatcc
     6361 ccctatccct tccaccacgt gttcaaggag gtacctgttc ttttccctgt cgagcggtcc
     6421 gaaatagagc ggatccatca gcgcgacagg ccgagcgccc atcgagagaa catcacgaac
     6481 gatgcctccc accccagtcg ccgcaccatc atggggatca acgtaactcg ggtgattatg
     6541 actctccata gagatggcaa gcgctgtcct ttcgctgaaa cggacaatcg ctgcatcatc
     6601 acctgggccg agtatgacat cggcgccgct ggtcgggagc gtttttaaca ggcgctttgt
     6661 tgatcggtaa ctgcagtgtt cactccaaag attttcaaag catgcgagtt cgacatcggt
     6721 aggttcccgt ctgagggtag ttcttatggt ggaaaggtcg gcggccgaca acatggtgat
     6781 agatgagctc tctctctgaa accataaagt tccatcgcgc agaggtagca tggtggggtt
     6841 tttacgggcc tggagggaat atataggaat atggttgatc catacgaggc actcctcaaa
     6901 aaggcatatt ccaatgtgac cgagcagagt gagtccacag aacggttcac cgtccctgat
     6961 gccagagtgt acattgaagg aaaaacgaca attcttgaaa attttgcaga gattgccgac
     7021 gtggttcgca gggatcagga ccatctcatg aagtatctgc ttggtgagct tggcacggca
     7081 ggaaaaattg acggaacacg cgctatcttc aacgggaagt tcgaatcatc cctgattaac
     7141 ggccttgtaa agggatatgt tgatgactat gtcatctgct ctgaatgcgg aaaaccagat
     7201 acccgccttg tgaaggatga cagggtcctc ctgctcaggt gcgacgcttg tggcgggcac
     7261 cggccggtac ggaaaaggaa ggcacgttct gagccggttg gaagtgatct ccaggaagga
     7321 caggaacttg acgttgaagt tcagtcggtc agtaaacgtg gagacggggt cgtgagaatg
     7381 ggtcggtata tcatgtacat ccctcgggga aaaccgggac aaaaagtaaa aatacggata
     7441 tcccggatat ctggctcaat tgtttttaca gaacagatat aattttttta ttgcagctgc
     7501 tacttttccg ggatcttccc cagcagttcc gtacaatacc ctgacaagcg ggtgcaggca
     7561 tcctttatcg cggcaagcgg atcacggtcc ccctttgtgg tgaccagcag ttcaggatca
     7621 gagaactgga atttgatcag ataccgggca acatcaacac cgggatcggt caggatctcc
     7681 tcagtaaggg tgttcatgaa cgtatgccct tcacctgaaa taatcagacg tgccttattc
     7741 ttctcaagct caagaatttt gatattcatc ccacctatca ttgagagatg aactggtata
     7801 taggtatgtg tagtccaacg agtgaaaatt tgagcgcaat caaggtctca ttgccgtgtt
     7861 gacgcatcca ctggggggtg acagctgttt tgtggagcca gtttttttaa aaacagaatg
     7921 tctctccatc tttccccgtg tgggggagat cccagggtat ggtgtggctc atatgaacac
     7981 atctgaaatc cccgaccatg agtctttttg caagagcaca ggcctcgtta tagttcatgt
     8041 gcttggggat ttgaatagat ggcgggacca gtgcgtcaac gaagaggaga tcggctttgg
     8101 aaaaaaccga aagtgtctcc ggcgggatat cagcatttga atcagaagtg aatgctattt
     8161 ttgcctttcc atcagtcagg acaaggccat aggtctcaac aggcggatga tttactggaa
     8221 ccagtgtcac ccggagtccg aagaggctga atggacggaa tggttctgta ggatttcgct
     8281 gaaacttcag gaacctgaaa atattcccac aatagtcgag cacttgtggg gcggcgtaga
     8341 cagcaggcat ggactgaacg cggtaaaatt caccaaaacc catgaagtga tcataatggc
     8401 catgggtcca tatcaccgca tcaatatggg gagatccata gaccagcagc tgctgcctca
     8461 aatcgggtga agagtctatc aggatagtgt gcccgttatg ctcgacaaga aaggaggtcc
     8521 ttagccgatg ggttccatga gtgagggcat atgtacattg cggacatgag catcctacct
     8581 ttggcgtccc gattgcatca cccgtcccca gaactgtaat gttcatccta taaccagttc
     8641 tggcggcgca ctgcacctct gttgcttacg agaatgattc cctgttcgat atcacaatcg
     8701 gtgatgtgag atcttccctc aatcagcgct gagagaagag cttcctttat cataatcctg
     8761 agatcagcac cggaaaatcc ttcagtgtta tcggcaacag ttccataatc gcactggcaa
     8821 tcgatggaac cggtgatgtt cctgatgatc tgggcccgca tttcgcgttc aggtagagaa
     8881 aactcaacta tctcatcgaa ccgccgccat gccgcttcgt caagcaactg aggatgattt
     8941 gttgcaccta tcaggagaac cccattccgg atgaggctga tcctgtcaat atttttcagg
     9001 agtgaattca cagccctctt catggcccca tgatcgtcgg taaccctgct cttggcaacg
     9061 aaatcgaatt catcaatgaa taggatacag ggagcaagcc tttttgcaaa atcaaagatc
     9121 ctgtcgatat ttttcgaggt ctctccaaga tactgggagg ttaccatgga gaggcgaact
     9181 tcaataagag ggaggtgcat ctcccgggat aatccaagag cgaggcttgt tttgcccgta
     9241 ccgggaggtc cgacaaagag gagttttcca acctcatgaa taccatgccg ctgcagggtc
     9301 tcacggtgtt cccgcgcggt tttgatcttg ctgatgatcc gcttctgccc atcggtgcag
     9361 acaaagtttt caaatgtttg ttccacctca tccggggcac ttatgatgac aaggtccatg
     9421 cccgatttga gggattcatc ttccgaaagg attgtagcta tcttcttatc gaggaaaagt
     9481 cgggtatctt cgtatggcgg attcgcagcc cggacaaggg ggtaagaggg agcagtggta
     9541 tcaagggatc ccaggtaata tgccaatgcc ggattccttc cggcaagatt gatgccccct
     9601 tttcgtacaa accatcgcac tgccgcatca aggcttgtca catggatacg ttggccgaaa
     9661 tcatcacacg tgataaccgg gattttggat agttttgaac aggcatcctg aataccgaga
     9721 ctacgcttga tcatgccttc actgaccgag agggggcgct ttacctctcc gttcccatca
     9781 acaccaaaca aaatccggca ttccggagtg agatcgttga tgtcaaggtt ctggtgctgg
     9841 ttgaatatct cagcagtgag cgccacctca agaagcttta acgtctcatc ctcttgggac
     9901 atctctgtaa catatcatat tggtaccgaa cttgcataag catatctcaa gcagtagtca
     9961 caatacagcc atcgctggta acaatgaccg tgtgctcgtg ctgggacacg agtgatccgg
    10021 ggacatcaga gaggacggga taagaacgga caatgccctg gctgatcagg cttgcaagcg
    10081 cgatatcctt ttttccatcg gttatccagc gccgtgcaaa cggcattcca tgccggtttc
    10141 gtaccgattc gagcaactta cgggcggaag gcagccgtac tggtttgact gctgtctgct
    10201 gaaagatctc aatgcgactc ttctcgctga ccctgccgga acctgtactg gcgaaaggtt
    10261 cgattgcaaa caccatcccc tcctccagca caaccccccc ctgaaacgag atgtttggga
    10321 tgctaggggg ggtatgtatc gagtacctgg caaggccgtg accggtgaga ttagaaacgg
    10381 gatgatatcc ccgcacttgg atctcatgct ggattgccct tccaaggtcc ccggatgtaa
    10441 cccctggtct gacaatctct attgcctttg tgagtgcagc ctttgatgct tcaatgagca
    10501 gtgcattgtc tccaaggtca accgtcacag cggtgtcagc gatataaccc tccagatgta
    10561 caccaaggtc aagcttcacc acatcacctt ccgagaatac cctctgctca tctgcagaag
    10621 ccgtatcgtg tgcggcatct tcgttaaacg agagattgag cgggaaggcc agcccgcatc
    10681 cctgttcttc aaccatctgc tcgatggact cgactgtttc aaggacccgg tttccaatct
    10741 ctatcaggga agatcctttt ttcaggataa ccgatgcgat ctttccggct tcactataac
    10801 agaagagaga atcatcgtcg ggaatcatag tcatatcacc agctcacacg gaaatttcgt
    10861 aatagtgcgg aaccctgttt gggttaccag tccaatattt tccagccgga tcccccctac
    10921 cttttcgtaa tagagtcccg gttcatttgt gacaacatgt cccctgctga gccgatcccc
    10981 ccccggcccg agagagggga gctcgtgaac ctcaagcccc accccatgtc cgagccccag
    11041 ttggctgccg attttgtgtg gttgtag
//
LOCUS       DAOOCO010000048        10680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5792_length_10680_cov_5.104659, whole genome shotgun sequence.
ACCESSION   DAOOCO010000048 DAOOCO010000000
VERSION     DAOOCO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5792_length_10680_cov_5.104659"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PK154_04115"
     CDS             complement(<1..1552)
                     /locus_tag="PK154_04115"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10280.1"
                     /translation="MLSCCGVANAYSISIESVHPTDSNYSHTWNITNPSATAIRVHFT
                     KIDLGTDPKQYQSGDTLVLKDQNGNIVHTYQGGDNYGANQDVLPDGGWSDWCVGNDIT
                     LTLTTKSGNTYGFTVDQMEIRETVAPINDLPESFHTTAAFYSHTWNITNPSATAIRVH
                     FTKIDLGTDPKQYQSGDTLVLKDQNGNIVHTYQGGDNYGANQDVLPDGGWSDWCVGND
                     ITLTLTTKSGNTYGFTVDQIDPPLLSQAPVANFVAVQTSDATQLLVQFTDQSTGTAPL
                     TYDWNFGDGATSTAASPSHTYASVGIYTVTLKVTNTDGENTAIQDVPVNTIPIPPPHT
                     YEGVYVPGGITWEDANADAIAKGGHLAVITSKEENELVFTTAASDDRLWKYENSRSWG
                     PWLGGYQTDGTQDPASGWTWVNGEAWNYANWETGEPNDQAGQSENRLLYFSLGNSKDL
                     TWNDGEKSSKLNGYILERGDFNSLSINSNPQGASIWVDGSDTGKVTPATIGVTEGSHV
                     IKIKMNGYC"
     gene            complement(2249..2665)
                     /locus_tag="PK154_04120"
     CDS             complement(2249..2665)
                     /locus_tag="PK154_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW10281.1"
                     /translation="MEPRINVITLGVRDFDRSLAFYRDGLGWEAQVQGDLALFSLNGM
                     IFALYPRESLADDANVPARETGFPGFTLAYLTRNEAEVDATLKKAGSVGACIVKPGQK
                     TFWGGYSGYFADPDEYLWEVAFNPFWKLDPEGRVRL"
     gene            2869..4338
                     /locus_tag="PK154_04125"
     CDS             2869..4338
                     /locus_tag="PK154_04125"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HPW10282.1"
                     /translation="MGKNIFSLYRVLIVTSALVILFLACSGCTENMGGGAMDKITTDP
                     GQTPEVMTSSATEPGSQENEPSLPASNTTTSSAPVSITVHSAFKTMKIKDTNPISGNI
                     FIVINTTIENLDEKIPYIVNETTVDITGGGPITRKVYGRLSNPFYWGSIPPKSSKTGE
                     MVFGVKATTEQFTLTLRDDQGKTILTKPLGSLPLGTYDQSPGSDSTTPESASAQTLNV
                     TIHSAQKTTKIQDTHPLPGNIYVVINMTIENLALSEPFVANENTIDITGGGPMTQKIY
                     DRVANPLYWGTIPPKSSKTGEIVFGVKESTNQFTLTLLDEKKQVLLSKPIGTLSTGPY
                     LPSLGNTGLLSATNFTSVIEGLDTPEKVTEYADARFTFTYHDGCISYPPEEFFRVQEG
                     DCKDYATFLSYVLAHHGYDTQIVAFKYFKDDKRYGHVVTLFKENDGSMYYVTTPVVSM
                     MRAVTSIDDLLKKECSRLGIPTIANYTIVPAGSVDTCVL"
     gene            complement(4639..5943)
                     /locus_tag="PK154_04130"
     CDS             complement(4639..5943)
                     /locus_tag="PK154_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW10283.1"
                     /translation="MSYLFEIIVILVLIAINGYFSLSEFAIISARRSRLQQRAEEGDV
                     RAGEAILLADKPTPFLSTIQVGITLVGIFTGAYGGATLAAALTPLIAEIPLLAPYSGL
                     ISITLVVLLITYLTLVFGELVPKRIALSNAESIAARVARPMSILSTIASPVVFLLSRS
                     TELFLRLIGIRESEPPPVTEDEIKIMLEEGTEHGVFDKAEVSMVEGIFELGDRNVASL
                     MTPRPDITGIDLEDSPETNYRIMADAGHSFFPAFRGDLDNIAGLIAVKDIWSALALGS
                     KPDLESVLRKPYFVPENTSIMKLLETFRESGLPMALVTDEYGSIRGLVTLHDILESIV
                     GGVRNLYRPEPPSIVVREDGSWLIDGDVVLEDLEEIIPVDDLPGKGSYQTLAGFLMYA
                     LQRIPSTGDHTEAEQFRFEVVDMDGNRVDKVLVSKVKEPNEK"
     gene            complement(6199..6528)
                     /locus_tag="PK154_04135"
     CDS             complement(6199..6528)
                     /locus_tag="PK154_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HPW10284.1"
                     /translation="MTISAWATLLVAGLMETGWAVGLKYSDGFTRLIPSLLTVGLMAG
                     SFYFLSLSLRDLPLGTSYAVWTGIGAIGTVLLGMVLFGESRETARLLCLFLIVAGILG
                     LRFVTGE"
     gene            6628..7104
                     /locus_tag="PK154_04140"
     CDS             6628..7104
                     /locus_tag="PK154_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10285.1"
                     /translation="MPGETFPREERYDALQRFFIRPFFLSLTIGIPFCIFKFLFGLTA
                     YRTGVLWLVIFGGIVVVWACLDILMNTGRSFLDLAGRNAPFEYCTIAQIGKLVHRPMV
                     FLAVDTLLSFVIICLMLWSGWITRLSQNELYLWYGATTLNLISLSLVSLYNEVRKS"
     gene            7387..8610
                     /locus_tag="PK154_04145"
     CDS             7387..8610
                     /locus_tag="PK154_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW10286.1"
                     /translation="MDYYPVIVIGGGPAGLFCGAEAAGSGQGVLLLEKMPSCGRKLLV
                     TGSGQCNLTHDGDIRDFFKKYGDHGAFLRSPLRKFSNHDLVAWFTSRDLPLAPDESGK
                     IFPKSRKAADVLSVLLAECRKNGVVIQYGEAVKEVRRSDTKFHVRTGTAEYESGALVI
                     ATGGSSYPATGSTGDGFSLAASLGHRIAPVAPALVPIRVRGYPFADLAGISFEGISLS
                     LFREGRKVREAAGDLLFTHMGVSGPGILHLSRYVLPGDILKISFLPGMRQELLTKDLV
                     DRIAVSGTKQVKSILSGYSLPARFIARILELLGLPGDLTGAHLPKKERAELVRMLTAF
                     PFVVSGTGDWNEAMVTRGGISLDEVDSSTMESLIVPRLYCIGEVLDIDGDTGGYNLQA
                     AFSTAMAAARDLATR"
     gene            complement(8614..10035)
                     /locus_tag="PK154_04150"
     CDS             complement(8614..10035)
                     /locus_tag="PK154_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW10287.1"
                     /translation="MTDPSDPLHPEAKPEHARAMTEGVALLMGDPKTAIRKLSLPMMA
                     AMLFMSVYNLINAIWVAGLGADALAAVGFMTPLFMVFIGLGNGLGAGVSSVVARKIGG
                     KNRQEAEHAAMHGLLIGAAIAVILTIPLILFAEPIAMLLGAGQAASLAAEYGRILFIG
                     TIFILFNNIAYAILRGEGDTKRTMYAMAFSSILNAVLDTVLIYWAGLGIAGAAVATVI
                     SIVSVTLIILYWFLVKKDTYLSITMKGFVPDRSVSRAILGVGLPASVEYLLMSALSII
                     INAILVMVAGTDAVAVYTSGWRLLMFAVVPEIAIGTTLISVVGAAYGARKYRNLALAQ
                     GYAIRLGIMIAAVMAVLIFVFAGEIALLFTYSETTAYLTPRIASFLRVISILLVFMPA
                     GMLSLSVFQGTGRGFTSLVINLFRGLAFVAVFVVLFGIVLGYGETGVWIGIVLGNILG
                     SMVAYLWARMYVRHLVLLEEQSA"
     gene            complement(10110..10325)
                     /locus_tag="PK154_04155"
     CDS             complement(10110..10325)
                     /locus_tag="PK154_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HPW10288.1"
                     /translation="MSSVKDGRSRDHIRPGIPVRIVQKKDQRSGRMTEGTVREILTGS
                     SFHPHGIKVRLTDGTVGRVQEIRNPEG"
BASE COUNT         2549 a         2866 c         2798 g         2467 t
ORIGIN      
        1 cgcaatatcc attcatttta attttaataa catgagagcc ttctgttact ccaattgttg
       61 caggggtcac ttttccggta tcagaaccgt ccacccagat tgatgcgcct tgaggatttg
      121 aattgattga aagagaatta aagtcccccc tttcaaggat atatccattg agtttgctag
      181 atttttcacc atcattccag gttaaatctt tcgagttccc aagggaaaag tacagtagtc
      241 gattttcact ttgacctgcc tgatcattag gttcaccagt ttcccagttt gcataattcc
      301 acgcttcacc gttgacccac gtccaaccac ttgcggggtc ttgagttcca tctgtctgat
      361 atcctcctaa ccatggaccc caagatctag aattttcata tttccaaagt ctgtcgtcac
      421 ttgccgcagt agtaaaaacc aactcatttt cttctttgct tgttatgact gccaggtgac
      481 ctccttttgc tattgcatca gcatttgcat cctcccatgt tattcctcca ggaacataca
      541 ctccctcata ggtgtggggc ggagggattg gtattgtgtt gacgggcacg tcctgtattg
      601 cggtattctc tccatctgta ttggttactt tcagcgtgac cgtatagatc ccgacacttg
      661 cataagtgtg agagggggag gcagcggtag aagtggctcc atccccaaag ttccaatcgt
      721 aggtcagcgg agcagtaccg gtggactgat cagtgaattg gactaataat tgtgttgcgt
      781 cagaagtctg cacagcaacg aagttcgcaa ccggtgcttg ggaaagtagc ggtggatcta
      841 tttggtcgac ggtaaatccg taggtatttc ccgatttcgt ggttagtgta agggtgatat
      901 cattaccaac acaccagtca ctccaaccgc catctgggag aacgtcctga ttagcaccgt
      961 aattatcccc tccttggtag gtatgtacaa tattaccgtt ctgatccttc agcaccagag
     1021 tgtcaccgga ttgatactgt ttcgggtctg tccccaaatc tattttcgtg aaatgaaccc
     1081 ttatcgcggt cgctgaagga ttcgtgatgt tccaggtgtg ggaataaaat gccgctgtgg
     1141 tatggaacga ttcgggtaaa tcattaatcg gggcgactgt ctcccgaatc tccatttggt
     1201 cgacggtaaa tccgtaggta tttcccgatt tcgtggttag tgtaagggtg atatcattac
     1261 caacacacca gtcactccaa ccgccatctg ggagaacgtc ctgattagca ccgtaattat
     1321 cccctccttg gtaggtatgt acaatattac cgttctgatc cttcagcacc agagtgtcac
     1381 cggattgata ctgtttcggg tctgtcccca aatctatttt cgtgaaatga acccttatcg
     1441 cggtcgctga aggattcgtg atgttccagg tgtgggaata atttgaatcg gtcggatgaa
     1501 ccgattctat tgagattgaa tatgcatttg caactccaca acaagaaagc atccccaaac
     1561 aggaacaaaa taaaaataaa aataataatt gtccacatat ttttttcata aaaaattaat
     1621 gtaagacttt taaccttaaa tcttttgaaa agctagttct ctctcacctt tctatttgcc
     1681 caggaatccc taattttata attcgaatgg ataatccgat tttttgtgat tacgctgtta
     1741 acccatgttt ttttatggac tattggcaca ggtcttcccc tccctggcag taatcaatcc
     1801 aggaaactct gcatcttctc tcatttctgg caggaaacaa accacttctg attcctgcag
     1861 catcctggaa cccctacagt aaaaccagcc ctgctggagg attgattgag ccatttttta
     1921 aagttatttt tctcattaaa gcacttttgg aggttttttc caaaggtgcc ttccgcacta
     1981 tggaactttg ggaggcacag atagaagggg atacctgagt tcctgcattt ccaggatact
     2041 gtgtgtatct ccaacctcca gagtattgat ctgtacatgt acgcgtatta ttaatagaga
     2101 gggaattttc aggattgaag cctgctgggt tgtttttgaa tccttcagca ggttcgatag
     2161 ttttgccgga aagctgctga aggattgaag gaacgagcgg aaagccctga ctgaagactt
     2221 gtgctatggt gtgatgtatg tgaaggaatt atagtctgac cctcccctcc gggtctaatt
     2281 tccagaaagg attgaaagcg acttcccaca gatactcatc agggtcggca aaatatccgc
     2341 tgtatcctcc ccagaatgtc ttttggccgg gtttcacaat acaggccccg acacttcctg
     2401 cctttttgag ggttgcatcc acttcggcct cgttcctggt gagataggca agggtgaatc
     2461 cgggaaagcc ggtttcccgt gcaggtacat tcgcatcatc agccaggctc tcgcggggat
     2521 agagggcgaa gatcatcccg tttagcgaaa agagagcgag atcaccctgc acctgcgcct
     2581 cccacccgag cccgtcacgg taaaaggcaa gtgaacgatc aaagtctctc acgccgaggg
     2641 tgatcacatt gatacgcggt tccatggtaa ccggattgtc aatggggtac tatatttcct
     2701 ttctgtctca ctaggagtca tgatacaccg gtcagggaat tatcctgccc tcattgaagt
     2761 gaattccctt ccgggcggaa gtacgcatgg aagagactga tcagaattga gcgatacttt
     2821 gaatgatatt tcataactct tatcccgcga aaatcgcaat tcctccgtat gggaaaaaat
     2881 attttttcat tataccgggt gctcatagtg accagtgcac tcgtcatcct ctttttagcc
     2941 tgttcgggat gcactgaaaa tatgggaggg ggggctatgg acaagataac aacagatcca
     3001 ggccagaccc cggaagtgat gaccagctcc gcaacagaac cagggtccca ggagaacgaa
     3061 cctagcctgc cggcttcaaa tacaacgaca agctccgccc ctgtcagcat caccgtccat
     3121 tctgcattta aaacgatgaa aatcaaagac acaaacccga tatcaggaaa tattttcatt
     3181 gtaatcaata cgacgattga aaatctggat gagaaaattc cttacatcgt caacgagact
     3241 accgttgaca ttactggggg agggcctatc acgcggaaag tctatggcag gttatcaaat
     3301 cccttctact ggggatccat tccgccgaaa agcagcaaga ccggtgaaat ggtattcggg
     3361 gtgaaagcaa cgaccgaaca gtttaccctc acgctgcggg atgaccaggg gaaaaccatt
     3421 ctcaccaaac cgcttggttc cctccctctg ggcacctatg atcaatcccc tggttcagac
     3481 agcaccaccc cggaatcagc atcagcccaa acccttaatg tcaccatcca ttctgcacaa
     3541 aagaccacga aaatccagga tacccatccc ctgcccggaa atatctatgt cgtcatcaac
     3601 atgacaatcg agaacctcgc tctcagcgaa ccatttgttg ccaatgaaaa taccattgat
     3661 atcacggggg gtggaccgat gacccagaag atttacgaca gagttgcaaa tcccttgtac
     3721 tggggaacga tcccgccgaa gagcagcaag accggtgaga tagtatttgg ggtaaaagag
     3781 agtacgaacc agttcacgct caccctcctt gatgaaaaga agcaggtcct tctctcaaaa
     3841 ccaatcggga cactctccac cggcccctac ctcccctccc ttggaaatac cggcttgctg
     3901 agtgccacaa atttcacttc ggtgattgaa ggcctggata ccccggagaa agttaccgag
     3961 tatgccgacg cgagattcac cttcacctac catgatggat gcatatccta tcccccggaa
     4021 gaattcttcc gtgttcagga aggagattgc aaggattatg cgacattcct ttcctatgta
     4081 ctcgcccatc acggttatga tacacaaatt gttgcattca agtatttcaa ggacgacaag
     4141 cgatacggac atgtcgtaac cctcttcaaa gagaacgacg ggtcgatgta ttatgtgaca
     4201 acccctgtgg taagtatgat gagggcggtc acctccatcg atgacctcct gaaaaaagaa
     4261 tgttctcgcc tgggtatacc aacgattgcc aattacacga tcgtccctgc aggctcggtg
     4321 gacacctgcg tcttatgatg cagggaacta ctattattta catctccaga actcctgaaa
     4381 agggctgata tcagcgcatc ctgtgttcga ctccctcata ccctcatttt cgaagggata
     4441 taccctgaca aaaaaaatgt tttttgtagg gttatcaccg gacaaaaaaa tacatggggt
     4501 accttcagac ccatgtaagc aagatgaacg tgccggaggc acttcgtata aaaaagagaa
     4561 atcttatgac ccgatgcagg agtctccagt ctgccggtca acaagttctg gtaatcaggc
     4621 gtcataacct cctccccatc atttctcatt cggctctttc accttcgaca ccagcacctt
     4681 gtccacccgg ttcccatcca tatccaccac ctcgaaccgg aactgctccg cctcggtatg
     4741 gtcccctgtc gagggaatcc gttggagggc atacatgagg aaccccgcca gcgtctggta
     4801 ggaacctttt cccggcaggt catccacggg gatgatctct tcgaggtcct cgagcaccac
     4861 atcaccgtcg ataagccatg acccgtcctc ccgcacgacg atggatggag gctcgggccg
     4921 gtagagattg cggactcccc cgacaataga ttcaagtatg tcgtggaggg tcaccagtcc
     4981 ccggatgctg ccgtattcat cagtcacgag cgccatcggg agcccggact cccggaatgt
     5041 ctcaaggagc ttcatgatcg aggtgttctc ggggacgaag tagggtttcc ggaggaccga
     5101 ttcgaggtcc ggttttgacc cgagtgcaag ggctgaccag atatctttga ccgcgatcag
     5161 acccgcgata ttgtcgaggt caccccggaa ggccgggaag aacgaatgtc ctgcatcggc
     5221 catgatgcgg tagtttgtct ctggggaatc ctcgagatcg atcccggtga tgtccggcct
     5281 cggggtcatg aggcttgcca cattccggtc cccaagttcg aagatccctt ccaccatcga
     5341 aacctcggcc ttgtcaaaga ccccgtgctc ggtcccctct tcgagcatga tcttgatctc
     5401 atcctctgtg acaggtggcg gctcggattc ccgtatcccg atgaggcgga ggaagagctc
     5461 ggtcgaccgg ctcaggagga agaccacagg ggaggcgatg gtggagagaa tcgacatcgg
     5521 ccgtgccacc cgtgcggcaa tcgattcggc attggaaagg gcaatccgtt tcgggaccag
     5581 ttccccaaag accagtgtca ggtaggtgat cagaagcacg accagcgtga tgctgatcag
     5641 gccgctgtac ggggcaagga gcgggatctc cgcgatgagg ggcgtgaggg cggccgcgag
     5701 cgtcgctccc ccgtatgctc cggtaaagat cccgaccagg gtgatcccga cctggatggt
     5761 tgagaggaac ggggtcggtt tatcggcaag gaggatggcc tcccctgccc gtacatctcc
     5821 ttcctctgca cgctgctgga gccgggatct ccttgccgag atgatggcaa attcggagag
     5881 ggaaaaatat ccgttgatcg caataaggac gagaatgaca atgatctcaa agagataact
     5941 cattttctgt tatagtgttc caaccgtgaa caaaaaagta tgcctcaggg agggagggca
     6001 tgttgcccgt tctctccggt tcacttttca ggtgtaccgg agagaatgca gatgaatgac
     6061 gcctatcccg tcggtggtgg aggctataag aaggactcgt ccctgttgca gggacacccc
     6121 ttcattgagg ctctttccca tttcaacaag cacggctgca ccccgctgac ctgctgcagg
     6181 tctcagggat ctgaaagttc actcccctgt cacgaacctg aggccgagga taccggcaac
     6241 gatcaggaag aggcaaagga ggcgggcggt ctcccgggac tccccgaaga ggaccatccc
     6301 cagaagcacg gtcccgatcg cccctatccc tgtccagacc gcataggagg tgccgagcgg
     6361 gaggtcacgt agtgagaggg agaggaaata gaaactcccg gccatcagcc cgaccgtgag
     6421 gagggaaggg atcaaccggg tgaaaccatc ggaatatttg agcccgaccg cccagccggt
     6481 ctccatgagg ccggcaacaa ggagggttgc ccatgcagag atcgtcatga tcatgggtct
     6541 ggatcgtgaa ggattaaaaa tcccactcgg caggaggatc tacgtcagct taatgcaatc
     6601 gggagcccaa cgtccctggt atccatcatg ccaggtgaga cctttccccg ggaggagcgg
     6661 tacgatgcac tccaacgctt cttcatccgc ccgttcttcc tctcgctgac cattgggatc
     6721 ccattctgca tcttcaagtt cctcttcggc ctgacagcat acaggacagg ggtactgtgg
     6781 ctggtgatct tcggagggat tgttgttgtc tgggcatgcc tcgatatcct gatgaacact
     6841 ggccgttcct tccttgatct cgccgggcgg aatgccccct ttgagtactg cacgattgca
     6901 cagatcggaa agctggtcca ccggcctatg gtctttcttg ccgtcgatac cctgctctcg
     6961 tttgtcatca tctgcctgat gctctggtca ggatggatca cgcgtctttc gcagaacgag
     7021 ctctacctct ggtacggtgc gactaccctg aacctgatca gcctgtcgct ggtctccctc
     7081 tataacgagg tcaggaaatc ctaaaaaaag gatcgccaga tgcaggcatt ccccccgatc
     7141 ctgatgccct gccttccggg agagcggagg agggggtttg catagtgctg gatcatatgt
     7201 gctcgaatga tgtggcatcg caaggacact ccggatacgt cagatgagcg gatcgttcgg
     7261 ggtggtcaga gttcacagtg aagaaccagg caaccatgat atagggaagg ccggccgcga
     7321 gcaggaacgc gagtactcac gcggctccga tacatcgaag agctgccgca acgagtaatt
     7381 cctgtggtgg actattatcc cgtgatcgtt attgggggag ggccggccgg gctcttctgc
     7441 ggagccgaag cggcagggtc cggccagggg gtgctcctcc tcgaaaaaat gccttcctgc
     7501 gggaggaagc tcctggtgac cggatccggg cagtgcaatc ttacccatga cggggacatc
     7561 cgggattttt tcaaaaagta cggggatcat ggcgcattcc tccgatcccc gctccggaag
     7621 ttctcgaacc atgacctcgt ggcctggttc accagccgcg acctccccct tgctcctgat
     7681 gaatccggta aaatcttccc gaagagcaga aaagcggccg atgtcctctc ggtgctgctc
     7741 gcggagtgcc ggaaaaacgg ggtcgtgatc caatatgggg aagccgttaa agaagtccgg
     7801 cggtccgata ccaaattcca tgtccggacc ggaactgcgg aatacgaatc cggagcgctc
     7861 gtgatcgcga ccggggggtc atcctacccg gccaccgggt cgaccgggga tggcttctcc
     7921 ctcgctgcat ctcttgggca ccgtatcgcc ccggttgccc cggcccttgt cccgatccgg
     7981 gtccgtggtt atccctttgc agaccttgcc gggatctcgt ttgaggggat ctccctctcg
     8041 ctctttcgtg agggtagaaa ggtccgggag gcagcgggag atctcctgtt cacccatatg
     8101 ggcgtatccg gcccggggat cctccatctc tcgaggtacg tcctgcccgg cgatatcctg
     8161 aagatctcgt tcctccctgg aatgaggcag gagctgctca ccaaagatct ggtcgacagg
     8221 atagcggttt ccgggacaaa acaggtgaag tccatcctct cgggatattc tctcccggcg
     8281 cggtttatcg cgaggattct cgaactgctc ggcctccccg gagatcttac cggtgcccac
     8341 cttccaaaga aggagagggc ggaactggtc cgaatgctca ccgcgttccc cttcgttgta
     8401 tccggtacgg gggactggaa cgaggcgatg gtgacgaggg ggggtatatc gcttgacgag
     8461 gtcgactctt cgacgatgga atcgcttatc gtccccagac tctattgtat cggggaggtc
     8521 cttgacatcg atggcgatac cgggggatac aatctccagg cagccttctc gaccgctatg
     8581 gcagcagcac gggaccttgc caccaggtag tgttcaggcg gattgttctt cgagcagtac
     8641 cagatggcga acgtacatcc gcgcccagag ataggcgacc atgcttccca ggatatttcc
     8701 aagcacgatt ccgatccaga cccctgtctc gccatacccc agcacaatcc cgaacagcac
     8761 aacaaagact gccacgaatg cgaggccacg gaagaggttg atcacgagtg aggtgaatcc
     8821 cctgccggtc ccctggaaga ccgacaggga cagcatcccg gccggcatga agaccagcag
     8881 tattgaaatg acccggagaa acgaggcgat ccgcggggtg aggtaggccg tcgtttccga
     8941 atacgtaaac aggagtgcta tctctccggc aaagacaaag atcaggactg ccatcactgc
     9001 cgctatcatg ataccaagcc gtattgcgta gccctgagcc agggccaggt tccggtattt
     9061 tcgggcgcca taagcggcac cgacaacaga gatgagcgtc gttccaatgg cgatctcggg
     9121 gacaacggca aacatcagga ggcgccagcc ggaagtgtat accgccacgg catcggtgcc
     9181 ggcgaccatg acaaggatcg cgttgatgat gatagaaagc gccgacatca ggagatattc
     9241 gacactggca ggcagcccca ctccaaggat agcccgggaa acagacctgt cagggacaaa
     9301 gcccttcatc gttatggaga ggtaggtatc cttctttacg aggaaccagt acaggatgat
     9361 gagcgtcacc gagacgatcg agatgaccgt cgccaccgca gccccggcaa tcccaaggcc
     9421 tgcccagtag atgaggacag tgtcaaggac ggcgttcagg atcgaagaga acgccatcgc
     9481 gtacatcgtc ctcttcgtat caccttctcc cctgaggatg gcataggcga tgttattgaa
     9541 gaggataaag atcgtcccga taaacaggat cctcccatac tcagctgcaa gggatgcagc
     9601 ctggccggca ccgaggagca tagcgatggg ttcggcgaat aggatcaggg ggatggtcag
     9661 gatcacggca atagcagccc caatcaggag cccgtgcata gcagcatgct cggcttcctg
     9721 ccggtttttc cccccaatct tgcgggcaac taccgaggag acccctgccc cgagcccgtt
     9781 tccaaggccg atgaacacca tgaagagcgg cgtcatgaac ccgactgccg cgagcgcatc
     9841 tgccccgagc cctgccaccc agatggcatt gatcaggttg tagaccgaca tgaagagcat
     9901 cgcggccatc atcgggaggg agagcttccg gatggcggtc ttcggatctc ccatgaggag
     9961 tgcgactcct tcggtcatcg cgcgggcatg ttccggcttt gcctccggat ggagaggatc
    10021 agaaggatcg gtcatggttc aatggctcct cgactcttca ggggtggtaa aaggggtccc
    10081 tggaccgaaa aatccggacc tgaccgtgtt cacccttcag ggtttctgat ctcctgcacc
    10141 ctgccgaccg tcccatcggt cagccggact tttatgccgt ggggatggaa ggaggagccg
    10201 gtcaggatct cacggacggt cccttcggtc attctcccgc tcctctggtc tttcttctgg
    10261 acaatccgga ccggtattcc aggcctgata tggtcccgcg accggccatc ctttacgctg
    10321 ctcatggact cgcttcccgt agtagccgct atatgaggtg gcgaaaaccc ttgaagggtg
    10381 cctgactggt atcacagatg cggcatgatt ctctatctat tggagttctt tggataaatg
    10441 cccacctgtg tgaaaagggt actctccggg gttacgccag atggaaagaa tcagagatga
    10501 acacgggaaa aatgagagtg cactatgctg gtgcagtcaa tgaagcgcac ctgtgcggga
    10561 agagctctct ccgttattac gcccgagtta aagaatcagc cataaacgct ggaaaatgag
    10621 agtgcagtac gctgatactg tcaatgcagg gcacttggca ggaagagggc aacttcaaag
//
LOCUS       DAOOCO010000049        10566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_5896_length_10566_cov_6.268290, whole genome shotgun sequence.
ACCESSION   DAOOCO010000049 DAOOCO010000000
VERSION     DAOOCO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_10566_cov_6.268290"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK154_04160"
     CDS             <1..684
                     /locus_tag="PK154_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10289.1"
                     /translation="GLCGPTGAGKSTIINILTRYYDIDSGSIEIDGQSIYDVTQESLR
                     RQIGVVLQEPFLFSDTVMNNLKYGRGGATDEECIQAAKDANCHEFITRLPKGYETILS
                     DGGRSLSQGQRQLLTIARAMIANPRILILDEATSNVDTRTERSIQSAIRKLQEGRTSF
                     VIAHRLSTITNAHQILVINNGEIVERGTHDEMMKAGGFYYNLYMSQFKGRVADILPGA
                     SSHSKGAPV"
     gene            779..1678
                     /locus_tag="PK154_04165"
     CDS             779..1678
                     /locus_tag="PK154_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPW10290.1"
                     /translation="MIKKKYIRRLMVRPGSKVSLKQYDTGWAQDDELKRVGKDVIKER
                     AAEILEANRASLAESQELLWASNLYSILIVLQGMDTSGKDGTIKHVMAGVNPQGCRVT
                     SFKVPTSEETDHNFLWRYTRALPARGEIGIFNRSYYEDVLVVKVHPEILDNQQLPPGK
                     RGDKFWNARYDDINAFERHLVQNGTFIMKFFLHISREEQKKRLLDRLEDKKKYWKFAL
                     SDLAERQFWDDYQTAYEMMLSKTSTDTAPWYIIPADYKWIARSLIADIISTKIRSLDL
                     MYPEVPEETLKQLEIAREKLASE"
     gene            complement(1953..4502)
                     /locus_tag="PK154_04170"
     CDS             complement(1953..4502)
                     /locus_tag="PK154_04170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPW10291.1"
                     /translation="MPPLDLARFTGLSSEEAQLRLLSDGPNELPSRETRGIIGIILDV
                     IREPMFLLLVGAGSVYFILGDIEEGALLLSFVLVIICITVYQERKTERALEALRNLSS
                     PRALVIRDGQLVRIAGRDVVRGDLLFTSEGDRIAADSLLLASTNLSVDESLLTGESVP
                     VRKLPAEGTTGERRPGGDDQPWLYSGTLVVQGQGVAEVVSTGMHTRMGEIGKALQTVN
                     QEETRLHSETAHIVRSIAFIGLFLFLVVVIVYGLTRGDWLHGLLAGITLAMAILPEEF
                     PVVLTVFLALGAWRLSKINVLSRHVPALEALGATTVLCTDKTGTLTVNRMTVAECVVA
                     GSSFDCLAEAGNPLPEPFHELLEYAILSCKKDPFDPMEQALLKTGTDDRSFTEHLHDD
                     WELLLEYPLSRELLAMSNVWRSRKGNEYIIAAKGAPEAIFDLCHLGDRVSSALTTQVV
                     DLAAQGLRVLAVAKSHFQPGQLPDGQHAFPFTYLGMIGFADPVRPDAAAAIHECRTAG
                     IRVVMITGDYPATAQNIARQIRLSDSEMVLTGSEIAALPANERDAKIQSTSVFARVVP
                     DQKLQIVESLKAGGEIVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREASSLVLLDD
                     NFASIVAGIRMGRRIYANLKKAMAYIISVHVPIAGLSLIPVLFGWPLILLPVQIVFLE
                     LIIDPACSIVFEAEQEEPDIMRQPPRDPEEHILDRRTVAIALIQGFVVLGIAFSIYVY
                     GMMRGLSADELRTLSFVTVVVANIALIFTNRSWTESIVAISRKPNKAFWLVSAGTVFF
                     LAAVLFLPGLRSLFQFAPVSPGFLLLCILGGMLTVGWFEIYKITEPRIRKT"
     gene            complement(4763..5656)
                     /locus_tag="PK154_04175"
     CDS             complement(4763..5656)
                     /locus_tag="PK154_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HPW10292.1"
                     /translation="MLIRDIAMRKTTLLRTLIHAPEILVIPVVHDPLCARIAEQAGIK
                     AVFSAGYANSAAYLGKPDVSLMSLSEMVSCASRIVDAVTIPVFADGDTGYGNVTNVIR
                     TIEQYEKAGVAGIFIEDQVFPKRCGHMDEKRVIPEREMVAKIRAAVATRKDPDLVIMA
                     RTDAIAIEGIDEAIQRANRYHDAGADLLFVEAPESVEQMRRICAEVKGPVFANNLPGG
                     KSPYLPAKELEDIGYAVVADATSCTYVIAKAVRELFTEISGSGSSAGLTDRMILFDEF
                     NRLVGLQEIREAEKFYYSGME"
     gene            complement(5779..6183)
                     /locus_tag="PK154_04180"
     CDS             complement(5779..6183)
                     /locus_tag="PK154_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW10293.1"
                     /translation="MVQMTPDIMEAFRSCKIFPLATASRDGEPNVAPMGAVFPRDPAT
                     IWIGNQFMKTTIRNIMENPRACLYVWGPGIKGCYKIKGDVMIKTAGDEYETMRREVAK
                     AKPGLVCRSLLVMRITEIYECMPGEHAGDRLV"
     gene            6440..7249
                     /locus_tag="PK154_04185"
     CDS             6440..7249
                     /locus_tag="PK154_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10294.1"
                     /translation="MDPNPDTNYPPLLEFRNVTIIKGGKSVLDNISVTLHQGEHIAIL
                     GPNGAGKSSFIKTINREYYPAVQDENGLIFRMRGRDVWNVWDLRALFGIVSNDLLFTF
                     TRDISGREVVLSGFFSSIGLFRHEVTASMEEKTDEILEFLEIDHLQQRPMTSLSSGEA
                     RRFLIGRALVHDPRTLIFDEPTNSLDLHSLHTFRSMLSKIAQSGTGIILVTHNLHDII
                     PEIRRVILMKDGRFWKDGLKTRILTDENLTELFRVPLHVLKEGGYFYATGY"
     gene            complement(7513..8673)
                     /locus_tag="PK154_04190"
     CDS             complement(7513..8673)
                     /locus_tag="PK154_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HPW10295.1"
                     /translation="MKNPFHVMLIPTLGCPAHCSYCWSSDAGSPKMSIDTVRDVVTWL
                     KEFRNDRATFTFHGGEPLLAGADFYRQALPMISRELSHLKPEFAMQSNLWLLTPEIAA
                     ILAEYQVPLGTSIDGPEELNDLQRGEGYFVKTMRGYEIARAHGLRVSFICTFTNYSVR
                     FREEIFNFFLENRFRLKLHPALPSLRDKNPEQWALDPDDYGDLLVFLLDKYLDNLDSI
                     EIMNINDLCRCIFTRRGSVCTFVNCTGNTFAVGPDGSIYPCYRFVGMPEWVMGHVRDA
                     PTMEELSCSGPWQRMHQYEEYVDEACKECKHLRYCRGGCPYNAIVPTGGKIAGVDPHC
                     RAYTRIFNEIGTRLNDEMMGTSFLDGVSSFQREQGARKRPGVMTLMHKVLSP"
     gene            8826..9020
                     /locus_tag="PK154_04195"
     CDS             8826..9020
                     /locus_tag="PK154_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10296.1"
                     /translation="MKDIVATRKMENGVAVYYPEGNDTKLESFNYSELIDLKINALDL
                     LENPKMYQVDPQKHRIVMKK"
     gene            9221..10513
                     /gene="rbcL"
                     /locus_tag="PK154_04200"
     CDS             9221..10513
                     /gene="rbcL"
                     /locus_tag="PK154_04200"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HPW10297.1"
                     /translation="MAIDWYMDFVDTGYIPAKDELICLYRFEPAAGISREEAVGRIAS
                     ESSTGTWTTLYSLPPMMKALQATAFEIEGTFVKIAYPLALWEEGNASQLLSGIAGNIF
                     GMKALKHLRLIDVTLPHEYLRHFKGPHFGMDGIREMMKVHGRPLTGAVPKPKVGFSAK
                     EHASVGFETWMGGFDFVKDDENLTSTSFNRFEDRVSALAKKRDRAEQLTGERKSAFIN
                     ITADTATMERRAALLAEYGWTYAMIDVVVAGTSAVSTLRDYCSDLGLAIHAHRAMHAA
                     FDRDPKHGISMQFIAKLMRLIGVSQIHTGTAVGKLIGSKKEVRLLADVLRNSLTKPSG
                     TILLEQDWGSMKSAFPVSSGGLHPGLVPDVLDIYGEDLVLLVSGGIHGHPDGTRAGAE
                     ATMQAIEAWKEGISLREKAAGSKELARALEKWGYYKPK"
BASE COUNT         2616 a         2789 c         2750 g         2411 t
ORIGIN      
        1 ggcctctgcg gtccgaccgg cgcagggaag agcacaatca tcaacattct cacccgctat
       61 tacgatatcg acagcggatc gatcgagatc gatggacaga gtatctatga tgtcacccag
      121 gagagcctcc ggagacagat aggggtggtg ctccaggagc cgttcctctt ctcagacacg
      181 gtcatgaaca acctgaaata cggacggggg ggagccaccg atgaagaatg tatccaggct
      241 gcaaaagacg caaactgcca tgaattcatc acccggcttc caaaaggcta cgagaccatc
      301 ctctcggatg gcgggcggag tctctcgcag ggacagcggc aactgctgac catcgcccgg
      361 gcgatgattg ccaacccgcg gatcctgatc ctcgatgaag caacaagtaa cgtagacacc
      421 cgcactgaac gatccatcca gtctgcaatc cggaaactgc aggagggcag gacgagcttt
      481 gtgatagccc accggctcag tacgatcacc aatgcccatc agatacttgt gatcaataac
      541 ggggagatcg tcgagcgggg cactcatgat gagatgatga aagcaggggg attctattac
      601 aacctttaca tgagccagtt caaggggagg gtggcagata tcctgccggg tgcctcttca
      661 cactcaaaag gggcaccagt atgacctcct ggggccaatc ctttcaaagg gtcatgcatg
      721 ccatggatgc acgagagaag cgcatgtgga ttcaggataa ctcaatcggg agagatcgat
      781 gataaaaaag aagtacatca ggagactcat ggtcaggccg gggagcaagg tatcgctgaa
      841 acaatacgat accggctggg cccaggacga cgaactgaaa agagtgggaa aggatgtgat
      901 aaaggagcgg gcagcggaga tactcgaggc aaacagggca tccctcgcgg aatcgcagga
      961 actcctctgg gccagcaatc tgtattccat ccttattgtt ctccagggca tggacacctc
     1021 ggggaaagac gggaccatca agcacgtcat ggccggtgtg aacccgcagg gatgcagagt
     1081 aacaagcttt aaggttccta cctcggaaga gaccgaccac aatttcctct ggcggtatac
     1141 ccgtgcactt cctgcacggg gagagatcgg tatcttcaac cgttcctatt acgaggatgt
     1201 actcgtagta aaagttcacc cggaaatcct cgataaccaa cagttgcccc ctggaaaacg
     1261 gggcgataaa ttctggaatg cgcggtatga tgatatcaat gcctttgaac gccacctggt
     1321 gcagaatggc actttcatca tgaaattttt ccttcatatc tcaagggagg aacagaagaa
     1381 gcggctcctg gaccgtctcg aggacaaaaa gaaatactgg aagtttgccc tgtccgatct
     1441 ggcagagcgc cagttctggg acgattacca gaccgcctat gagatgatgc tctccaagac
     1501 gagcaccgat acagcgccat ggtacatcat acctgcagat tacaagtgga tagcccgtag
     1561 ccttatcgcg gatatcatct ccacaaagat ccggtcactc gatctcatgt atcctgaagt
     1621 tccagaagaa accctcaaac aactcgaaat tgcccgggaa aaactggcca gtgagtgact
     1681 ctttttttgt gtctgataca ttaccagtag gtttcataag gatattataa gagatgaggc
     1741 taattttcca ttttttttga ggatataaga tcgtcaattc cccttcggaa agcaaggata
     1801 gcctcctcca ccatgaccgt tttcattctt tgctgatcgg tatcattcgg tcagcaggaa
     1861 ggatatttac ccagaatcta ttctgccaga tgccacccag ggtggtttca gaccatttcg
     1921 tgaaggttga agggaatggc aatcgtgagg attcaggtct tcctgattct tggttcggtg
     1981 atcttgtaga tctcgaacca tccaacggtg agcatccccc cgaggataca aaggaggagg
     2041 aatccaggag agactggtgc aaactggaac agagatctca gtccagggag gaacaagacc
     2101 gcggcaagga agaacaccgt tcccgcgctg accagccaga atgccttgtt gggttttctg
     2161 gaaatggcta cgatactctc tgtccaggaa cggttggtaa atatcaaagc gatattggca
     2221 accacgaccg tgacaaatga gagggtgcgc agctcatccg ctgaaagtcc cctcatcatt
     2281 ccgtagacat atatcgagaa tgcaatcccg agaacgacaa atccctggat aagtgcaatt
     2341 gctaccgtgc ggcgatcaag gatatgctct tccggatctc gtggtggctg tcgcatgatg
     2401 tccggctcct cttgttcggc ttcaaacacg atcgaacagg cagggtcgat gatcagttca
     2461 aggaacacga tctggacagg gaggaggatc aatggccatc cgaaaaggac cggaatcagt
     2521 gacagccctg caatggggac atggacggag atgatatatg ccatcgcctt cttcagattc
     2581 gcatagatcc tcctccccat tcgtatccct gcaacaatcg atgcaaaatt atcatccagg
     2641 agtaccagcg aagaagcttc cctggctaca tcggtccctc tccctcccat cgcaatgccg
     2701 atatgggcag ccttcaatgc cggggcatca ttcactccat ccccggtcat ggcaacgatc
     2761 tcaccgccgg cctttaatga ctcaacgatc tggagtttct gatcaggaac cacccgcgca
     2821 aagacagagg tggactggat cttcgcatct ctctcatttg ccggtaatgc cgcaatttca
     2881 gagccggtga gcaccatctc actatccgaa agcctaatct gccgggcaat gttctgagcc
     2941 gtggcagggt aatcaccagt aatcataaca accctgatac ctgcggttct gcattcgtgt
     3001 attgccgctg ctgcatctgg ccgcacggga tcggcaaatc cgatcatgcc gaggtaagtg
     3061 aaggggaatg cgtgctggcc atcgggcagt tgtcccggct gaaaatggga ttttgcgact
     3121 gccaggacgc gaagtccctg cgctgcaaga tccacaacct gggtagtcag tgccgagctc
     3181 accctgtctc ccagatggca gagatcgaag atcgcttcag gtgcaccttt cgcagcgatg
     3241 atgtattcat tcccctttct tgaccgccac acattggaca ttgccaggag ctcgcgggag
     3301 agcgggtatt cgagcaggag ttcccagtca tcatgaagat gctcggtaaa agatcgatcg
     3361 tcagtcccgg tcttcaacag tgcctgctcc atcggatcga agggatcttt cttgcaggag
     3421 aggatcgcat actcgaggag ctcatggaac ggttccggca gcggattgcc ggcctcggca
     3481 aggcagtcaa aagaagaacc ggccacaaca cactctgcaa cggtcattcg gttcacggta
     3541 agagtaccgg ttttatcggt gcagagcacc gttgttgcgc cgagggcttc gagtgcagga
     3601 acatgccggg aaaggacgtt aatctttgaa agcctccagg caccaagggc aagaaaaacc
     3661 gtcaggacaa cgggaaattc ctccgggagg atagccatgg caagggtgat tcccgcaagg
     3721 agcccgtgga gccagtcccc ccgtgtcaag ccatagacga taacgacgac aaggaacagg
     3781 aacaggccga tgaaggcaat gctcctgacg atatgtgctg tctcggaatg gagcctggtc
     3841 tcttcctgat tgacggtctg cagtgccttt ccaatctctc ccatccgtgt gtgcattcct
     3901 gttgatacca cctctgcaac accctgtccc tggacgacga gagttccaga gtacagccac
     3961 ggctggtcat cccctcccgg tctgcgctca ccggttgtgc cttccgcagg gagtttccgt
     4021 acggggaccg attctccggt gaggagggat tcatccactg aaaggttagt ggatgcaagg
     4081 agaagggagt ctgcggcgat acggtctccc tcgctcgtaa aaaggagatc gccccggact
     4141 acgtcccgtc cggcaatcct caccagctgc ccatccctga tgaccagtgc cctggggctg
     4201 gataggttcc tcagtgcctc aagggcacgt tcggtcttcc gttcctggta gaccgtgatg
     4261 catatgataa ccaggacaaa tgagaggagg agggcacctt cctctatgtc accgaggata
     4321 aaatagacgg agcctgctcc cacgagcagg aggaacatgg gctcccggat gacatcgaga
     4381 atgatgccga ttattccgcg ggtctcgcgt gaagggagtt cgttcggacc atcactgagt
     4441 aaccgcagct gagcttcttc ggacgataat ccggtaaatc gagcaaggtc aagggggggc
     4501 atgcagcaat tcgatacttc atacgataag ttggcctgac ccataggttt taccctgcgc
     4561 tgaaatcatg acaggcgtgc ccggtgtatt tctcttcccc tgtcagtgtc aacagggtat
     4621 tgaaaaaaat aacatgattc ctttgagggc cacaagcata aaagatgaaa atgccggagg
     4681 cattttcata taaaaaagaa tccggggtct tttccccatt cggttcaggg attgtcaagg
     4741 gagctccaga gctccaaccc catcactcca ttcctgaata gtagaacttc tcggcctccc
     4801 tgatctcctg aagaccaacc agtcggttaa actcatcgaa gaggatcatg cgatcggtca
     4861 atcctgcaga agatccgctc ccggatatct cggtgaacag ttcccggacc gcctttgcta
     4921 tcacataggt gcatgaagtg gcatcggcga cgactgcata cccgatatcc tccagctcct
     4981 tcgcaggaag atacggtgat ttacccccgg gaaggttgtt ggcaaagaca ggccctttca
     5041 cttcagcgca tatccgccgc atctgttcta ccgattcggg ggcctcgaca aacagaagat
     5101 cagcacctgc atcatggtag cggttagccc gctgaatcgc ctcatcaatt ccttcgattg
     5161 cgattgcatc cgtccgtgcc atgatcacga gatcgggatc ttttctggta gcgactgcag
     5221 cacgtatctt tgccaccatc tccctctccg gaatcacccg tttttcatcc atgtgaccac
     5281 accgtttggg aaaaacctgg tcttcgatga aaatacccgc cactcctgct ttctcatatt
     5341 gttctatggt cctgatgaca ttcgtcacgt tcccgtatcc tgtatccccg tctgcgaaga
     5401 ccgggatggt gacagcatcg acgatccggg atgcacaact gaccatctcg gataacgaca
     5461 tcagggatac gtcaggtttt ccgagatagg cagccgagtt tgcataccct gccgaaaaaa
     5521 cggctttaat tcctgcctgt tcggctatcc tcgcgcagag agggtcatga acgacaggaa
     5581 tgacgagaat ttcaggagca tggataaggg ttctcaggag tgtggtcttc ctcatggcaa
     5641 tatccctgat cagcatcagg gccacggcat atgtacgttt cgaaaaagag agaaactggg
     5701 aatgaccctt ctgcagccag gaagcacgga ggaatatctt tatcgggatg aaaaagggct
     5761 tctggaatcc ttcgcttctt ataccagccg gtcaccggca tgttcgccgg gcatgcattc
     5821 gtagatctct gtgatcctca tcacaaggag cgatcggcag acaagccctg gctttgcctt
     5881 tgccacttcc cggcgcatcg tctcatactc gtctcccgca gtctttatca tcacatcccc
     5941 tttgatcttg taacatccct ttattccggg gccccagaca tagagacatg cccttggatt
     6001 ctccatgata ttcctgatcg ttgtcttcat gaactggttc cctatccaga tcgtagcagg
     6061 atcgcggggg aagacagcgc ccatgggtgc tacattcggt tcgccatccc tcgatgcggt
     6121 tgccagaggg aaaattttgc atgatctgaa ggcttccatg atatcgggtg tcatctgtac
     6181 catgggtatt ttgtctgtcg aggagcccga taaaatgagc gaaatggctg gcgtatccac
     6241 ggcactcatc cagcgggatt tcccttcggg gatactagga cggatgcgga agagagtttg
     6301 catccagcat tcagtccttt ttatcgggtc atcatcccgg gatcgggaaa atctgaaaac
     6361 agggaaaggc atcgtagttt taatcggttc agacatatga tgtatactca cagccagaat
     6421 gcgatggaaa taattcctta tggatcctaa tcccgatacc aactaccccc cgctcctgga
     6481 attcaggaat gtcaccatta tcaaaggggg gaaatcagtg ctcgacaata tctcggtaac
     6541 ccttcaccag ggtgagcata ttgccatcct cggtccgaac ggtgcgggaa agtcctcatt
     6601 catcaagacc atcaacaggg agtactaccc ggcggtgcag gatgagaacg gcctgatatt
     6661 ccggatgcgg ggtcgcgatg tctggaacgt ctgggattta agagcactgt tcgggatcgt
     6721 gtcaaatgac ctgttgttca cttttacacg ggatatttcc gggcgcgagg tggtattatc
     6781 cgggtttttt tcgagtatag gactcttccg gcatgaagta acggcaagca tggaggagaa
     6841 gaccgatgag atcctcgagt tccttgaaat agaccacctg cagcaacggc cgatgacctc
     6901 gctctcctcc ggtgaagccc gtcggtttct tatcggcaga gccctcgtcc atgatccaag
     6961 gaccctcatt tttgatgagc ccaccaacag cctcgatctc cattcactgc atacgttccg
     7021 ttcgatgctg agcaagattg cgcaatccgg caccgggatc atcctcgtga cccataacct
     7081 ccatgatata atccctgaaa tcagacgggt gattctgatg aaggatggca ggttctggaa
     7141 ggatgggctg aaaaccagga ttctcactga tgagaatctg acggagctgt tcagggttcc
     7201 cctccatgtg ctgaaggaag gaggatactt ctacgcgacg ggatactgat ccttattccc
     7261 cctcctctcc cccaatacac ctcctctgtt gtgggaaggc tctggtgctt ttgagacacc
     7321 tcctttttga aatacctctt tcttagtaca ccccctgttc gacagtattc ttttgcacga
     7381 tgcatcacca atggggtatc gcaattatcc cagcccattg tgggacgttc agaaaagaga
     7441 acccatagag gcaggaatac aaaataatgg gaaccaggta ccccgggatt tttttgccgt
     7501 gcacatcctc atttatggcg atagtacttt atgcatcagc gtcattaccc ccggcctctt
     7561 tcgtgcaccc tgttctctct ggaatgatga aaccccatct aggaatgaag tacccatcat
     7621 ctcatcgttg agccgtgtgc ctatctcatt gaaaatccgt gtatacgccc tgcaatgggg
     7681 atcaacgcct gcaatcttcc ctcccgtggg gacgatagcg ttatatgggc atcccccgcg
     7741 gcagtaacgg aggtgtttgc actccttgca cgcttcatcc acgtactcct catactggtg
     7801 cattctctgc cacggacctg aacatgacaa ttcctccatt gttggcgcat cccggacatg
     7861 acccattacc cattccggca tcccgacaaa ccggtaacag ggatagatac tcccgtcagg
     7921 gcccaccgca aatgtgtttc ccgtgcagtt cacgaacgta cagaccgatc ccctccttgt
     7981 gaaaatacac cggcagagat cattgatgtt catgatctcg atcgaatcca gattatccag
     8041 atatttatca aggaggaaga cgagcaaatc gccatagtca tccggatcga gtgcccattg
     8101 ttccggattt ttatcacgca gcgagggaag ggccggatgc agtttcaacc tgaaacggtt
     8161 ctcaaggaag aagttgaaaa tttcctcacg aaatcggacg gaataattgg taaaggtaca
     8221 gatgaagctg acccgcaggc catgggctct tgcgatctca tacccccgca tcgtctttac
     8281 aaaatagcct tctcccctct ggagatcgtt taattcctcc ggaccatcga tgctggtccc
     8341 cagggggacc tggtactctg caagtatagc ggcaatttcc ggggtgagga gccacaggtt
     8401 ggactgcatc gcaaactcgg gcttgaggtg gctgagctct cgggagatca tgggaagggc
     8461 ctgccggtag aaatcggcac cggccaggag gggttcgccc ccgtggaagg tgaatgtggc
     8521 acgatcattc ctgaactctt tcagccaggt gacaacatcc ctgacggtgt cgatactcat
     8581 ttttggtgat cctgcatcag agctccagca gtagctgcag tgtgcaggac aacccagggt
     8641 cggaatgagc atcacatgga acggattttt catggcagcg tacaccactg tcccttttca
     8701 aagatgttaa cggttttctt ctttcccccg ttcccctgaa aaaaggtaat caatgatcgc
     8761 ttcgtgcaga aagaggcctc atctggaggg atagtatttt attgaactcc ggtaatcgct
     8821 atcatatgaa agacatcgtg gcaaccagaa aaatggagaa cggggttgca gtgtattatc
     8881 ccgaagggaa cgatacaaaa ctggaatcat tcaactattc agaactgatc gatctgaaaa
     8941 ttaatgccct tgatctcctg gaaaatccga agatgtacca ggtggaccct caaaagcata
     9001 ggattgtaat gaaaaaataa tctccttctc ttattcccac gccggattgc accggcagga
     9061 catctggatg agcatcctgg ccgtctcatc aggaatcgtt ctcctgtagt ctgccagggt
     9121 ttctctccct caatttggaa gagctgcatc ggacccggtg agggaccaga atttctgtgc
     9181 agacttatca ggtttcagat ccttttagta gtgagatgca atggcaatcg actggtacat
     9241 ggattttgtg gacaccggct atatcccggc aaaggatgag ctgatctgtc tttatcgttt
     9301 cgagcctgca gcagggatca gcagggagga agcggtaggc cggattgcat ccgagagttc
     9361 aacgggcaca tggacgaccc tctactccct tcccccaatg atgaaagcgc tgcaggcaac
     9421 agcgtttgaa atcgagggca cctttgtgaa gatcgcgtat ccactcgctc tttgggagga
     9481 agggaacgcc tcccagctgc tgagcgggat tgccggaaac atcttcggta tgaaggcgct
     9541 gaaacaccta aggctgatcg atgtgaccct tcctcacgag tatttacggc atttcaaagg
     9601 ccctcacttc ggcatggacg gtatccggga aatgatgaag gtccatgggc gaccccttac
     9661 aggggctgtc ccgaaaccca aggttggatt ctctgcgaaa gaacacgctt cagtcggatt
     9721 cgaaacctgg atggggggat ttgattttgt caaggatgac gagaacctga cctcgacctc
     9781 cttcaaccgg tttgaggatc gggtatcagc cctcgcaaaa aagagggacc gggctgagca
     9841 gctcaccgga gagagaaaat cggcgttcat caatatcact gccgatacgg cgaccatgga
     9901 acgtcgcgcc gccctcctcg ctgagtacgg ttggacttat gccatgatcg atgtcgtggt
     9961 cgcaggtact tctgcggtct cgacactcag ggattactgt tcagatctcg ggctggcgat
    10021 ccatgcccac cgtgccatgc atgctgcatt cgatcgggat ccaaaacacg gcatctcgat
    10081 gcagttcatc gcaaaactga tgcggcttat cggcgtgagc cagatccata ccggtacagc
    10141 ggttggaaag ctgatcggat cgaaaaaaga agtgcgactc cttgctgatg tccttcgcaa
    10201 ttcactgaca aagccctcgg gcactatcct ccttgaacag gactggggga gcatgaagag
    10261 cgcctttccg gtctcgtctg gaggtctcca tcccggcctt gtcccggatg ttctggatat
    10321 ctacggtgag gatctcgttc ttctcgtaag cggtggaatc catggacatc ctgacgggac
    10381 aagggccggg gcggaagcga caatgcaggc catcgaagca tggaaggaag ggatttctct
    10441 ccgtgagaaa gcagcgggat caaaggaact cgcacgggcg ctcgaaaagt ggggatacta
    10501 taagccgaaa taattacccg gcgctgcctg ccccggaggc atatcccgca gaatcagtat
    10561 tcaatg
//
LOCUS       DAOOCO010000050        10376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6060_length_10376_cov_5.054161, whole genome shotgun sequence.
ACCESSION   DAOOCO010000050 DAOOCO010000000
VERSION     DAOOCO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6060_length_10376_cov_5.054161"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PK154_04205"
     CDS             complement(<1..826)
                     /locus_tag="PK154_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPW10298.1"
                     /translation="MGFINYDVNRYSPKQMVAIPLVLLILSLAVLGYTLATTGMPVTP
                     GMDFSGGTAVTVFTSDSPDSLSATFAGYPLISIGEGVSDGKYIKFGPMDDDSLQSLAT
                     LINERYPDAKIDQIGASFGETLQKQAAFALILSFIGMSIVVFLAFRTFVPSAAVVLSA
                     FADIAMTAAVMTVIGIELTLPTTAALLMLIGYSVDSDILLTMRVLKRQGKLDEKLAGA
                     FRTGITMTTTTMAAVAAMWAVSYFGNIPVIPEISAVLFIGLIIDMMNTWLTNAGIIK"
     gene            974..1282
                     /locus_tag="PK154_04210"
     CDS             974..1282
                     /locus_tag="PK154_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HPW10299.1"
                     /translation="MRSPHEIKGVIEIRLKNYLSRDRTGVRRALLHLFLQVKCLTISD
                     IFAILARKFSISYHSLAAMVGLIASKIGILHITKSREGSCSVYSLKEQYAGMVKRIVS
                     "
     gene            1334..1633
                     /locus_tag="PK154_04215"
     CDS             1334..1633
                     /locus_tag="PK154_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10300.1"
                     /translation="MHARESHHSSIGEIRITDEVIAFIMKRKCDFRISTSCGGPILLP
                     VSIKPPKPTDIQIPAGDYTIFVSVHQARYLSTVHMGLIPYFMDEIKEESNCPYEL"
     gene            1593..2030
                     /locus_tag="PK154_04220"
     CDS             1593..2030
                     /locus_tag="PK154_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPW10301.1"
                     /translation="MRSRKNRIALMSFEELEHTADIRIRVRGATLEELFSEAARALMT
                     IIYGNTSPGTIRHHIEVDADDTISLIQEFLSEILFVSEVEEMVITGADIHLAGNHLTA
                     NLTGEPFSRMKHCGGREVKGISYSDLTIRHEEDSYILEVIFDV"
     gene            2037..3470
                     /locus_tag="PK154_04225"
     CDS             2037..3470
                     /locus_tag="PK154_04225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPW10302.1"
                     /translation="MLPGIKRVAPLEWEVPIGTVPGMRVPGRFFLSDELAKTLEEGAV
                     QQLANVATMPGIVKNSLAMPDIHWGYGFPIGGVAAFSIEEGVISPGGVGFDINCGVRI
                     LTTPLHEKDIVKKRELIERLFNAVPTGVGAKSTMRLSPKELEGMLIRGAKWAVDQGFG
                     MESDLVHCEEQGAMAGADPSAVSAKARQRGMPQSGTLGAGNHFLEIQTVREIFQDGAA
                     GAYGLFPGQICIMIHCGSRGLGHQVCTEHLKVLEGATKKYGIVLPDRQLACAPITSSE
                     GRAYFGAMAASSNYAWANRQVIMHMVRKVFSAMFDIQYEEMNLVYDVAHNVAKLEEHV
                     VDGRPMKLCVHRKGATRAFGPGCADLPREYAPWGQPVIIPGSMGTSSYLLHGTNEAMV
                     RTFGSTCHGSGRIMSRTKAKKALSGREVRESLAAEGIIIRAPHDGAIADEAPEVYKPS
                     DEVVRIVDSAGISRLVARFVPLGVIKG"
     gene            3536..4042
                     /locus_tag="PK154_04230"
     CDS             3536..4042
                     /locus_tag="PK154_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10303.1"
                     /translation="MPCEHVTPHMVAAPFFRGRFVTLIIPTGFANPGYSCLLPALKAS
                     SSRISRFVSLGGHLLVFGASSEREDAYDWLPQQIRYRHDYGCRNLEIDTSHYASSLLE
                     GYDTHAVECDGYFTEYEGRIVASSCGNPVMIVQNIGRGEIVVTTVHEYPSKVFLKTFC
                     SAPEETLF"
     gene            4075..4497
                     /locus_tag="PK154_04235"
     CDS             4075..4497
                     /locus_tag="PK154_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HPW10304.1"
                     /translation="MNRFIISTGSTADDLIPVGMALHEMLNRLPITARSKQKPGVRIE
                     SGTIVDREYTGPVLEQVLEENKVLKVTPKSGAYKGVPVVVSPIRDSTGDAIVAIGVVD
                     ITGIFDLATLMEHQAFILEQVCGKDPCPLPSEQINAKR"
     gene            4503..5480
                     /locus_tag="PK154_04240"
     CDS             4503..5480
                     /locus_tag="PK154_04240"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPW10305.1"
                     /translation="MYDAAFKVLEVLEGAEGPVSGAYLSSKLGISRSAVWKHIKELIS
                     MGYEISASQKEGYRLTKKSNRLLPYEVHKKLRTSFIGKKIRYFENTPSTIWVGKQIAS
                     EGDAGLLHGMVIIAEEQTGGIGRLGRSWVSPIGGVWITIVLKPRIPIDRVFMVTMAAS
                     VAVARAIRKEYDLGALIKWPNDIYIGDKKVAGLLLELFAEADDIHYCLLGIGVDVNIS
                     PQDLEGVKSAVTSISAEMGHDVDRASFLARLLREFESRFLLIEAGEYEAVTREWKSLS
                     CTLEHRVRINTMKATFEGEAIDIDEFGALLIRKDNGKIERVIGGDCIQR"
     gene            5519..6067
                     /locus_tag="PK154_04245"
     CDS             5519..6067
                     /locus_tag="PK154_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPW10306.1"
                     /translation="MFHAPERALLLTETAAFTGLIALGSWISIPFVPVPFTLQTLFVL
                     LSGAVMKRYALLPTGLYVLMGFIGIPVFHNGGAGLGILLGPTGGYILGFIPGACITGI
                     LFERCERSARLLGILLGDGCIFLCGIAWLILSTGLSPSIAFLVGVLPFIPGDVVKGYA
                     VWVIGERLDRVNTMKREEVPGT"
     gene            6064..6747
                     /locus_tag="PK154_04250"
     CDS             6064..6747
                     /locus_tag="PK154_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HPW10307.1"
                     /translation="MIIVRNLVFRRLSIPELTIPPGYVSLIGPNGSGKTTFLRVLSGV
                     LKHAEGAALIDDTPLRERRIGFVNEYPDRNILFSRVSDELASTLRFSHLPCEEITRKV
                     DLTIERAGLSHLADRYIHTLSGGEKVLVALLSATIHSPEVLLLDEFDSHLDEHAVDAA
                     EQVISSSGAVYVIRCTQNMDIASRSETLIALFNGRIRSIGAPSEVFSALSGTCYVPFA
                     WRSRIETGP"
     gene            6704..7429
                     /locus_tag="PK154_04255"
     CDS             6704..7429
                     /locus_tag="PK154_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10308.1"
                     /translation="MSPSHGGLGLKLVLDRIIMKRGSWSCAADGILVPGIHLISGRVG
                     SGKTTLAELICGLLEPTSGELVLDGISSWMLCQQFPEYHVIGATLEKEAQSYGVVPEL
                     ALDRAGLTGRGGEDPFCLSRGELRKLQFSCLSLCDRDLFVWDEPYSALDCREKQRVAQ
                     HLSTRSSGITILFTHEQRYFPKTDYLWEMCDGVLKYVGKVPEALPLWEHAPSHIRFLI
                     GKGVIPANLTEEDLGEALCRMQG"
     gene            7411..8064
                     /locus_tag="PK154_04260"
     CDS             7411..8064
                     /locus_tag="PK154_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10309.1"
                     /translation="MQDARLRLFSVLLLSAATYLSIAGALLTLLWLFLYPTSLVSSVK
                     SKALYILLPFTGIVALVMQIIGENGISYFIRIGILLLLAFTIFQGWTPGEFLDLAVWL
                     FGTGIGFDIGLAIELTIQGIKDADQDWSRILTAMKLKGMHPEFRNLASIGFLMIQLRL
                     LRADDQADLLTTRGYKKGGSCCPHFEPSNRDIIASLCAILILLVSFMAVRDIFILMM"
     gene            8119..9849
                     /locus_tag="PK154_04265"
     CDS             8119..9849
                     /locus_tag="PK154_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HPW10310.1"
                     /translation="MSTRLLITDTTLRDAHQSLIATRMKTEDMIPLARTIDKVGFFSV
                     EAWGGATFDVSIRYLAEDPWDRVRMLKEELKHTPIQMLLRGQNLVGYRHYPDDVVDRF
                     VTHASSVGVDIFRVFDALNDIRNMRESMKMVKEVGAHLQGSICYTTSPVNSIPGFIEM
                     VKELYSFNCDSICIKDMAGLIMPHAARDLITGIKDEIDIPVDLHSHSTSGIASMSYQA
                     AIEAGVDIVDTAMSPFALGTSQPPTESVVASLKGTSRDPGLDLHLLLDVRDICSKMRE
                     KYGGILDPISERVDSNVLLYQLPGGMISNLVSQLREQDALDKLDDVFAEVPRVRADLG
                     YPPLVTPTSQIVGTQAVLNVLLGGDRYRNVTNEVKDYVRGLYGKPPAPISDEVRKLII
                     GDEPVITVRPADLLEPVFEKMKEQAIADGLIKKGDEDVLTIILYPAIAPSFLKGERKP
                     EELPVKNQRQSSPADIPHAMEVEVDGEVFNVRILSVGGSAVASSAAPAQKIPRGDIQG
                     GIKSNMQGMVLEVAVSRGSEVKKGDLLVVLEAMKMENPIHSPTDGRVSDIFVNAGDVV
                     QSGDVLLVVE"
     gene            9846..>10376
                     /locus_tag="PK154_04270"
     CDS             9846..>10376
                     /locus_tag="PK154_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPW10311.1"
                     /translation="MKYFEKVLIANRGEIAIRVMRACRELNIETVAIYSNADKDALHV
                     KYADEAFLVGEAPPSKSYLNIERIVDIAKKSGSEAIHPGYGFLAENYHFAERCTDEGI
                     TFIGPSWRTIRAMGSKIESKQMMREAGVPVVPGTPGGVKEPDEARKIAEEIGYPVIVK
                     ASAGGGGIGMQIVNDET"
BASE COUNT         2523 a         2491 c         2819 g         2543 t
ORIGIN      
        1 acttgatgat gcccgcattc gtcagccagg tattcatcat gtcaatgata aggccgataa
       61 acaggacagc ggaaatttca ggaataaccg gtatatttcc gaaatagctg accgcccaca
      121 ttgcggcaac agcggccatc gtagtggtag tcatggtgat gccagtcctg aacgccccgg
      181 cgagtttttc atcaagtttt ccctggcgtt tcagtacgcg catcgtcaag agaatatcac
      241 tgtcaacaga gtacccgatc agcataagga gtgctgcggt agttggcagt gtcagctcta
      301 tcccgattac ggtcataact gcagcagtca tcgcaatatc agcaaaggct gagaggacga
      361 ccgctgctga aggaacgaag gtccggaaag ccaggaatac cacaattgac atgccaatga
      421 aagacaatat cagggcaaag gcagcctgct tctggagtgt ttccccgaat gaagcgccga
      481 tctgatcgat ttttgcatcg ggatatcgct cgttgatcag ggtagcaagg gactggagag
      541 aatcatcatc catggggccg aatttgatat attttccatc gctgacccct tcgccgatac
      601 tgatcagcgg gtatcctgca aatgtggcag agagactatc agggctgtca gaagtaaata
      661 ccgtgacagc cgtccccccg gaaaaatcca tccccggcgt taccggcata cctgtggttg
      721 ccaacgtgta gccgagcacc gcaagggata gtatcaggag aaccaggggg attgccacca
      781 tctgctttgg ggaatagcgg ttcacatcgt aattgataaa tcccataaca ctaaggttcg
      841 taacatacga ttaaaaggtt ttagataatt ctcccccaaa tctgctcaaa tggtagattt
      901 acaataaaag agcggaactg gaggccggga aaaaaacaaa aatattatat aaacttggcg
      961 gtattagctc agtatgagat ctccacatga gatcaaagga gtcatcgaga tccggctgaa
     1021 gaactacctt tcccgggaca ggactggtgt caggcgtgca ctcctccatc ttttcctcca
     1081 agtgaaatgc ctgaccattt cagatatttt tgccatcctt gcccggaaat tctccatcag
     1141 ctatcattct cttgcggcga tggtgggact gatcgcctca aagatcggaa ttcttcatat
     1201 cacaaaaagt cgggagggat cctgcagtgt atattccctc aaggaacaat atgcaggaat
     1261 ggtgaaaagg attgtttcct gaagggaagg gaacactttt ttcatgattg gctgctcatg
     1321 atgtgagcag agcatgcacg cacgagaaag tcatcattct tcgattggag agatcaggat
     1381 taccgatgag gtgatagcct ttatcatgaa gcggaaatgt gattttcgca tttctacttc
     1441 ctgcgggggc ccaatcctcc tgcccgtcag tataaaaccc ccaaaaccaa cggatattca
     1501 aatccctgca ggggattata ccatattcgt ctctgttcat caggcacgat atctctcgac
     1561 cgtgcatatg ggtctgatcc cttattttat ggatgagatc aaggaagaat cgaattgccc
     1621 ttatgagctt tgaagaactg gaacataccg cggacatcag gatacgggta cggggtgcaa
     1681 cgctcgaaga gcttttttcc gaagcagccc gggcactcat gacaataata tacggaaata
     1741 caagtccggg aacaatccgg caccatatcg aagtggatgc cgatgacaca atctctctca
     1801 tccaggagtt tctctctgaa attctttttg tttccgaggt agaggagatg gtcatcaccg
     1861 gagcggatat ccaccttgcg ggcaatcatc tcaccgccaa tctcaccggg gaaccatttt
     1921 ccaggatgaa acattgcggt ggcagggagg tgaaggggat ctcctactca gatcttacca
     1981 tccgccatga agaggactct tatatacttg aggtcatctt tgatgtgtga agagccatgc
     2041 tccccggaat aaaacgtgtc gccccacttg aatgggaggt gccgatcgga acagtccccg
     2101 gcatgcgggt gccgggcagg tttttcctgt cagatgagct tgccaaaacc cttgaggagg
     2161 gtgcggtgca gcagctcgca aatgttgcaa cgatgccggg aattgtgaag aattcgcttg
     2221 ccatgccgga tatccactgg ggttacggtt ttcctatcgg tggtgttgct gcgttttcga
     2281 ttgaagaggg ggtcatctct cccgggggtg ttggattcga tatcaattgc ggtgtcagga
     2341 tcctcaccac cccgctccat gaaaaggata tcgtgaagaa acgggaactg atagaacgtc
     2401 ttttcaacgc ggtacccact ggggtgggtg caaaaagcac catgcggctc tcccctaaag
     2461 agcttgaggg gatgctcatc cgaggagcga aatgggcagt cgatcagggt ttcgggatgg
     2521 agagcgatct ggtccactgc gaggagcagg gagccatggc tggtgccgat cccagcgccg
     2581 tttctgcaaa ggcccgccag cgtggcatgc cccagagcgg gactcttggg gcaggaaatc
     2641 atttcctgga aatccagaca gtccgggaga tttttcagga tggggcggct ggtgcgtacg
     2701 ggctttttcc cggccagatc tgtatcatga tccattgtgg ttcacggggg ctgggacacc
     2761 aggtctgtac tgaacatctg aaggttctgg aaggggcaac aaaaaaatat ggaattgtcc
     2821 ttcctgaccg gcagcttgcc tgtgcgccta ttacctcatc ggaaggaagg gcgtattttg
     2881 gtgcaatggc agcctcatcg aactatgcgt gggcaaaccg gcaggtgata atgcatatgg
     2941 tgcggaaggt attctccgcg atgttcgata tccagtatga ggaaatgaat cttgtttatg
     3001 atgtagccca caatgtcgcc aaactagagg agcatgttgt cgatggcaga cccatgaagc
     3061 tttgtgtgca tagaaagggt gcaacccgtg cattcggtcc cggatgtgct gacctgcccc
     3121 gggaatatgc cccttgggga cagccggtca ttatccctgg gagcatggga acctcctcat
     3181 atcttcttca cggtacaaat gaagcaatgg tgagaacgtt cgggtcaacc tgccatggat
     3241 cggggaggat tatgagccgt acaaaggcca agaaagcgct ctcaggaagg gaagtgagag
     3301 aatctcttgc tgctgaaggg atcattatcc gtgccccgca tgatggcgct attgccgatg
     3361 aagcgccgga ggtctacaag ccaagtgatg aagtggtcag gattgttgat tcggccggga
     3421 tttcgcgcct ggtcgcgaga tttgttccgc tgggagtgat caaagggtga tatgcagggc
     3481 aggactcctg tgggattcaa agctgctctt ttcccgttac atagaggaga gtggcgtgcc
     3541 gtgcgagcat gtcacccctc acatggtagc tgcgcctttc ttccggggac gatttgtcac
     3601 cctgattatc cctacgggtt ttgcaaatcc gggatactcg tgcctcctcc ctgcactgaa
     3661 agcttcatcg tcccgaatca gtcggttcgt ctctcttggc gggcatctgc tcgtctttgg
     3721 cgcatcatct gaacgcgaag atgcgtacga ctggctcccg caacagatcc gatatcgcca
     3781 tgattatggg tgccggaatc ttgagattga taccagccac tatgcttctt cccttttgga
     3841 gggatatgat acccatgctg tcgaatgtga tggatatttt accgaatacg aaggccgcat
     3901 tgttgcttct tcgtgcggaa atcctgtaat gatagtacaa aacattggga ggggcgagat
     3961 tgtggtcaca acggtgcatg aatatccttc aaaggtcttt ctgaaaacat tctgttcagc
     4021 accggaggaa acattatttt gactggaagc gtggattcta tgagagaggt accgatgaac
     4081 cgtttcatca tctcaaccgg gtccactgcc gatgacctta tccctgtagg aatggcattg
     4141 catgaaatgc tcaatcgcct tcccattact gcacgatcaa agcaaaagcc tggagtccga
     4201 attgagtcgg gaactatcgt ggacagggag tatacagggc cagtgctcga acaggttctg
     4261 gaagagaata aagtgttaaa agtaaccccg aagtctggcg catataaggg tgttcccgtt
     4321 gtggttagtc caatccgtga cagtactggt gatgcaatcg tcgctatcgg ggtcgttgat
     4381 atcacgggaa tatttgatct tgcaacgctt atggagcacc aggcgttcat cctcgagcag
     4441 gtatgcggaa aggatccctg cccactcccg tccgaacaga tcaatgcaaa aaggtaatga
     4501 gaatgtacga cgcggcattc aaagttcttg aagttcttga gggagcagaa ggtcctgtct
     4561 ccggagcgta tctcagcagc aagctgggga tatcgcgatc agcagtctgg aaacacatca
     4621 aagaactgat cagcatggga tatgagatct cggcttcaca aaaagaagga taccgcctca
     4681 caaagaaaag caaccgactt ctcccatacg aggtccataa gaaactcagg accagcttta
     4741 ttgggaaaaa aatacgctac ttcgaaaata ctccttcaac tatttgggtg ggaaagcaga
     4801 ttgcgagcga gggggatgcc gggcttctcc atggcatggt tatcattgct gaagagcaga
     4861 cgggagggat tggaagactc ggacgatcct gggtatcgcc aatcgggggt gtctggatca
     4921 ccattgtact caaaccccgc attcccattg atcgtgtttt catggttacg atggccgcat
     4981 cggttgctgt cgctcgtgca atcaggaaag aatatgatct cggcgcgctg atcaagtggc
     5041 cgaatgatat ctatatcggt gacaaaaagg tggcaggtct ccttctcgaa ctctttgccg
     5101 aagcagatga tatccactac tgtctgcttg ggataggtgt tgatgttaat atctctcccc
     5161 aggatctcga gggggtaaag agcgcggtca cctcgatctc ggccgaaatg ggtcatgatg
     5221 ttgaccgggc atcgtttctc gcacggctgc tccgtgaatt tgagagccgg tttctcctta
     5281 tcgaagcagg ggagtatgag gcagtgaccc gggaatggaa atccctctca tgtacattgg
     5341 agcaccgcgt acggatcaat accatgaaag cgacttttga gggagaagca attgatattg
     5401 atgagtttgg agcgctcctc atcaggaaag acaatggtaa gatcgagagg gtgattggcg
     5461 gggactgtat ccagcggtaa tactttatcg tcaacaagaa aaaaatatag ggttgacgat
     5521 gttccatgcc ccggagcggg cgctccttct cactgagact gctgcattca ccgggctcat
     5581 agcattggga tcctggatat ctattccatt cgttcccgtt cctttcacgc tgcagacact
     5641 gttcgttctc ctctccgggg ctgtcatgaa gcgctatgcc cttttgccca caggattata
     5701 tgtcctgatg ggtttcatcg gtatccctgt ttttcataac gggggtgcag gtctggggat
     5761 cctgcttggg cccacaggag ggtatatcct tggattcatt cctggtgcat gcatcacagg
     5821 aattttgttc gaacgttgtg aacgatctgc aaggctcctc ggcatactgc tcggagatgg
     5881 gtgcattttt ctttgcggca tcgcatggct gatactctcc accgggcttt ctccctctat
     5941 cgcatttctt gtcggagtgc tccctttcat tcctggggac gtagtgaaag gatacgccgt
     6001 ctgggttatc ggagagcgcc tggatagggt gaatacaatg aagagagaag aggtgcctgg
     6061 cacatgatca ttgtccggaa cttagtcttt cgaaggctct ccatcccgga actcacgata
     6121 ccccccgggt atgtgtcatt gataggtcca aacggcagcg ggaagacgac gtttctccgg
     6181 gtgctttcgg gggtcctgaa acatgcagaa ggggctgccc ttatcgatga tacgccactc
     6241 cgggaacgcc gtattggttt tgtaaatgag tatcccgaca gaaatatcct tttttcgcgg
     6301 gtttcagacg agctggcatc aacgctgcga ttttctcatc tcccttgtga ggagattacc
     6361 cggaaagtgg acctgactat tgagcgtgca gggctctccc acctcgctga tcggtatatc
     6421 cataccctct caggagggga gaaggtgctg gtagcacttc tttctgctac tatccattca
     6481 ccggaagtac tccttctgga cgaatttgat tcacaccttg atgagcatgc agttgatgct
     6541 gcagaacagg tcatcagcag ttctggagca gtatacgtaa tacgatgtac ccagaatatg
     6601 gacattgcgt cccggtctga gacgttgatc gcccttttta atggccggat ccgttcaatt
     6661 ggtgctcctt ctgaagtatt ttccgcgctt tccgggacct gttatgtccc cttcgcatgg
     6721 aggtctcgga ttgaaactgg tccttgacag gatcatcatg aagcggggat cttggtcctg
     6781 cgccgctgat ggtatcctgg ttccggggat tcacctgatt tcagggaggg ttggatcagg
     6841 gaaaacgacc ctcgccgagc tgatctgcgg gcttctggaa ccaacatccg gggagcttgt
     6901 ccttgatgga atctcatcct ggatgctctg ccagcagttt cctgaatacc atgtcatcgg
     6961 agcaacgctg gagaaagagg cccaatcata tggggttgtc ccggaattag cgcttgatag
     7021 agctggattg accggaaggg gtggggaaga tccattctgc ctttcccgcg gggagctccg
     7081 gaagttacag ttctcatgcc tctcactgtg tgaccgggat ctgtttgttt gggatgaacc
     7141 atatagtgcc cttgattgcc gtgagaagca aagagtggca cagcaccttt caacccgctc
     7201 ttctgggatc acaatactat tcacccacga acagcgttat ttcccaaaga ccgattacct
     7261 ctgggagatg tgcgatggag tccttaaata cgttggaaaa gtgcctgaag cacttccctt
     7321 gtgggagcat gcgccctctc atatcaggtt tctcatcggc aaaggggtaa tcccggcaaa
     7381 cctgacagaa gaggatttgg gggaagctct gtgcaggatg caaggctgag gctcttctcg
     7441 gtactcctcc tttctgcggc tacctacctc agtatagcag gagcactgct cactttgctc
     7501 tggctttttc tctacccaac atctctcgtc tcctccgtca agtcaaaagc attgtatatt
     7561 ctcctccctt tcaccggaat cgtcgctctg gtcatgcaga tcatagggga gaatggaata
     7621 tcgtacttta tccggattgg catcctcctt ctcctcgcgt tcacgatatt ccaaggctgg
     7681 acaccggggg agttccttga cctggcagtc tggctctttg gcacagggat cggattcgac
     7741 atcgggctcg ctatcgaatt aacgattcaa gggatcaaag atgctgacca ggactggtcc
     7801 cgtatcctta ccgctatgaa actaaagggg atgcatcccg aatttaggaa cctggcttct
     7861 atcggattcc tgatgattca gctccgcctg ttacgggcgg atgaccaggc agatctgctc
     7921 actacccggg gatacaaaaa agggggaagc tgctgcccgc atttcgagcc atcaaatcgt
     7981 gacataattg cgtcattgtg cgcaattctc attttactcg tttcttttat ggcggttcgt
     8041 gatattttta tacttatgat gtaaaagatt taaaggtttg aaaaaatttc tcctttatca
     8101 tcgagccgga gtaacctgat gtccacaaga ctgctgatca ctgacaccac cctgcgggat
     8161 gcccaccagt ccctgattgc cacacgcatg aagaccgagg acatgatccc ccttgcccgc
     8221 actatcgata aggttggatt cttttcagtg gaagcatggg gtggagccac gttcgacgta
     8281 agcatccggt accttgcgga ggatccatgg gacagggtgc ggatgctcaa ggaagagctt
     8341 aagcacaccc cgatacagat gctcctccgt ggccagaacc ttgtcggata ccgtcattat
     8401 ccggatgatg tcgttgaccg tttcgttacc catgcctctt cggtcggcgt tgatatcttc
     8461 cgcgttttcg atgccctgaa tgacatcagg aatatgaggg aatcaatgaa gatggtgaag
     8521 gaggtggggg cacacctcca gggctcaatc tgttacacga cgagtccggt caactccatt
     8581 cctgggttca tcgagatggt caaagaattg tattctttta attgtgactc catctgcatc
     8641 aaggacatgg cgggactgat catgccccat gcggcaaggg atcttatcac cgggatcaag
     8701 gatgagatag atatcccggt cgatcttcac agccattcca caagcggtat tgcttcgatg
     8761 agttaccagg cggcaatcga agccggggtg gatattgttg ataccgcaat gtcccccttc
     8821 gcgcttggga cctcccaacc tccgactgaa agtgtcgtgg catcactcaa agggacctcc
     8881 cgggatccag gacttgatct tcatctgctg ctcgacgtca gggatatctg cagtaaaatg
     8941 agggaaaaat acggggggat actggatcca atctcagagc gggtcgatag taatgtcctg
     9001 ctataccagt taccaggcgg catgatatca aatctcgtct cgcaactgag agagcaggat
     9061 gctctcgaca aactggatga tgttttcgct gaagtacctc gggtcagagc agatcttggc
     9121 tatcctcccc tggtgacccc cacctcgcag atagtgggaa cccaggcagt actgaatgtg
     9181 ctcctcggag gtgaccggta ccggaatgtt acgaacgagg tgaaggacta tgtccgtggg
     9241 ctctacggaa aaccccccgc tcctatcagc gatgaggtca gaaaactcat tatcggagat
     9301 gaaccggtta ttacggtccg gcccgctgat cttctggaac cggtattcga aaaaatgaag
     9361 gagcaggcaa tcgcagatgg cctcataaag aaaggggatg aggacgttct taccattatc
     9421 ctctatcctg caatcgcccc ttcattcctg aaaggtgagc ggaaacctga ggaactccct
     9481 gtgaaaaatc agcggcagtc ctcaccagct gacatacctc atgccatgga ggtggaagtg
     9541 gatggcgagg tcttcaacgt ccgcattctc tctgtcggtg gcagtgcggt tgcctcatca
     9601 gcagctcctg ctcagaagat accccgggga gatattcagg gcgggataaa atcgaatatg
     9661 caggggatgg tacttgaggt tgcagtaagc cggggatctg aggtgaaaaa aggagatctg
     9721 ctcgtcgtcc ttgaggcaat gaagatggag aatccgatcc attctccgac tgacggcaga
     9781 gtctctgata tttttgtgaa tgcaggggat gtggtgcaga gcggggatgt gctcctggtg
     9841 gtggaatgaa atacttcgaa aaggttctca ttgcaaaccg tggagagatt gcaatcaggg
     9901 tgatgcgggc ttgcagggaa ctgaatatcg aaacagtggc catctactca aatgctgata
     9961 aggatgccct tcacgtcaaa tacgctgatg aggcatttct cgtgggggag gccccgccct
    10021 ccaagagcta cctgaatatc gagcggattg ttgatatcgc aaaaaaatcc gggagtgaag
    10081 cgatccatcc gggatacgga ttccttgccg agaattacca tttcgcagaa cgatgcacag
    10141 atgaagggat cacgtttata gggccttcct ggcggacgat cagggcaatg ggatccaaga
    10201 tcgagagcaa acagatgatg cgagaagcgg gagtccctgt tgtaccgggt acccctggtg
    10261 gggtgaagga gccggatgaa gcacggaaga tagctgaaga gatcggctac cctgtcatcg
    10321 tgaaggcaag tgctggtggc ggcggaatag ggatgcagat agtcaacgat gaaaca
//
LOCUS       DAOOCO010000051        10373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6065_length_10373_cov_5.179589, whole genome shotgun sequence.
ACCESSION   DAOOCO010000051 DAOOCO010000000
VERSION     DAOOCO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10373
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_10373_cov_5.179589"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK154_04275"
     CDS             complement(<1..592)
                     /locus_tag="PK154_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPW10312.1"
                     /translation="MGSMEALGTSGIPLVAAFFIGLVMTFSPCPLATNITAIAFISKK
                     IGDSRHTLLVGTLYALGRMVAYIGLTALIVYAGLNIQSISLFLQEYGEKLIGPFLVIM
                     GILMLELVDIPSIGGQGWLQKIELYLGEKGYLGGFLLGIIFALALCPFAAVLFFGMLI
                     PIALKTGDAIFVPAVFGIATALPVIIISLVLVSGVNR"
     gene            complement(601..1080)
                     /locus_tag="PK154_04280"
     CDS             complement(601..1080)
                     /locus_tag="PK154_04280"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPW10313.1"
                     /translation="MMNEIMKRRIVVLGILLLLVTLAALGAGCTAPAATNESQIMTTP
                     GAVQKVEVYHFHRTQQCYSCRTVGAYAEDTVKTYFAPELESGRVVFGHINIEEPENKA
                     LVDQYGPTGSSLWIGVYDKDGFHKEENVNVWYKIGDKEAYLAYLKGVIEKRMAGDFS"
     gene            complement(1070..1486)
                     /locus_tag="PK154_04285"
     CDS             complement(1070..1486)
                     /locus_tag="PK154_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPW10314.1"
                     /translation="MAEPDFVRGSSLDLDDRPVLLLTCSGISHTGQLTTMAGNSLRCR
                     HPVLVSRHVRLTSLTRSLEKELTGDECLIAVDGCEECCAKKRMDLIGKIPDAYLIATR
                     EGITKRGMEEPRYDEIECLCQAVVRTIRKSGGKNDE"
     gene            complement(1493..2269)
                     /locus_tag="PK154_04290"
     CDS             complement(1493..2269)
                     /locus_tag="PK154_04290"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPW10315.1"
                     /translation="MNESIGCIGGGRVVRILLHGWREAGLLPENIIVSDPNRGALDQI
                     TAEFPGVTVLQDQNALAAGQDLVIIAVHPPDAGKVLGVIAPALKPDAIVISLMPKLTI
                     AQISGMLGGFDRIVRMIPNAPSMVLSGYTPVTFPGGIAMEDRAMVGTLLESLGRYIDV
                     PEQDLEAYAVISAMGPTCLWFQLYELEQIAVSFGLSPERAADAVRKMAEGTVQTMMWS
                     HLTPEQVMDLVPVRPLGEDEPAIREMYRRRLPALYQKLKG"
     gene            complement(2277..2720)
                     /locus_tag="PK154_04295"
     CDS             complement(2277..2720)
                     /locus_tag="PK154_04295"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPW10316.1"
                     /translation="MKRNLIIEWKHIGTTVTGTCERCSLTGGAIMDVLEDLQPYFRQK
                     NITARFRETILPDSQIEESNQVLINGVPLEEYLSDARVVQTPCCSCACITGQDEAECR
                     AIDVGEERYEAIPPDLLKRVIIGVVESWETPCSCGCDCGPDMKCE"
     gene            complement(2717..3898)
                     /gene="arsB"
                     /locus_tag="PK154_04300"
     CDS             complement(2717..3898)
                     /gene="arsB"
                     /locus_tag="PK154_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPW10317.1"
                     /translation="MPGESCVTKEPKGLGWFERYLTVWVLLCIGIGILLGNYAPELAR
                     YLDSLAIYSGEAPIISLPIAIALFFMMYPIMVKIDFAEVLKAGKNIKPVALTLFVNWA
                     IKPFTMYAIALFFLGYVFIGFIGPEAVDLVKMPFGLDLPVGAPYGDGTVVMVDGIKML
                     EIPLWRSYLAGCILLGIAPCTAMVLVWGYLAKGNDGHTLVMVAINSLTMLLLYGPLGG
                     FLLGIGQLPVPWQALALSIAVYVALPLVAGYISRRLIIRAKGEEWFNRKFVHYLTPVT
                     IIALLSTLVLLFTFKGEVILSQPLTIVWIAIPLFLQTMLIFTIGYFLAKKIHLTYEDA
                     APSAMIGASNHFEVAIATATILFGIASGAALATVVGVLIEVPTMLLLVKICLRTQGWF
                     R"
     gene            complement(3888..4310)
                     /locus_tag="PK154_04305"
     CDS             complement(3888..4310)
                     /locus_tag="PK154_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPW10318.1"
                     /translation="MASRPECACGDTSALAGGKRRIIFPCAGTANVGLISNVAALQLA
                     LEGYGVATCIALLGSGDPGLKKRIAEADEVVLIDGCPMTCGAKIANAQGVTPAQHIVI
                     TGLGIDKVYEKNFTEDEVELVVSAAWEGRGRPGETHAR"
     gene            complement(4314..4547)
                     /locus_tag="PK154_04310"
     CDS             complement(4314..4547)
                     /locus_tag="PK154_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW10319.1"
                     /translation="MKIEVLGTGCMKCKRLFKNVEIAVKELGITAEIVKVDDISEIIN
                     RGVMLTPALAVDGEMKVSGRVADVKELKEILKG"
     gene            complement(4544..4816)
                     /locus_tag="PK154_04315"
     CDS             complement(4544..4816)
                     /locus_tag="PK154_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10320.1"
                     /translation="MAPVDEKPCCEAEALRRIRQVDVGGIVVGISMLDHILTEVKAME
                     LPDEKETGRELLKRVKIYNYIPASAEEKYRIALLQEYQKCKGGGTI"
     gene            complement(4800..5903)
                     /locus_tag="PK154_04320"
     CDS             complement(4800..5903)
                     /locus_tag="PK154_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPW10321.1"
                     /translation="MTDAITGALLSGLNAVIEYLAAHVITCLVPAFFIAGAIAVFVKK
                     EAILKYFSPDAKKSVSYGVASVSGVVLAVCSCTILPMFAGILKKGSGIGPATTFLYAG
                     PAINILAIIYTASVLGWDIGFARAFFAITMSIAIGLLMATIFRSYDEAVTAAGKGFVA
                     GSEESTRPKWVVPLFFVFLVLILVIGASGLIPFSVRLSIVYVLTLGVALLLIYYFERD
                     EVTEWGYEIWDLTKKIFPILIAGTFIVGIIAFFLPPETFKPFFGDNSLGACLLASVVG
                     ALLYMPTLLEVPIIGGTFGYTSGVMAGGPALSLLLAGPAVSLPSLLVLYRIMGGKKTL
                     VYALLVVGFSTFAGFVFGSYFPYLGIPGCGTSG"
     gene            6131..6523
                     /locus_tag="PK154_04325"
     CDS             6131..6523
                     /locus_tag="PK154_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW10322.1"
                     /translation="MARKDVDDLSIPQEIADSLCSCGGVEGLIDQIPSDQMFEQMQGW
                     YKACADPVRLKILSLLMVQPLCVCVIKAVVRMADSKLSYHLNILKKAGMIEGEQQGQY
                     IIYQITPRAKVFMEKEVKRYESEKARDE"
     gene            6495..6848
                     /locus_tag="PK154_04330"
     CDS             6495..6848
                     /locus_tag="PK154_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10323.1"
                     /translation="MSLKKPETNKNCGTMTASSVDLLNVAECSPDTPVKKDLIKMSGS
                     NIVLVCLETGQEIPPHPEPYAVVFVVLQGEGIITAGSARHPVKPLHLVSVRKDENRGI
                     RCDQRMVLLGIRDDV"
     gene            6861..7790
                     /locus_tag="PK154_04335"
     CDS             6861..7790
                     /locus_tag="PK154_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10324.1"
                     /translation="MPDAIVVADLTKVFGSTVAVDHVSFLVKEGEIFGFLGPNGAGKT
                     TTTRMLTGVIPPDSGSAMILGHDIQREPVLAKQGFGVVPETSNAYTDLTAWQNLMLMG
                     ELYGISPVRVSERASELLTVLGLFERKDQKVQAYSKGMKQRLILAMALLHEPRLLFLD
                     EPTSGLDVQSTQMILSLLRDLNRAGTTIFLTTHNMEEANRLCNRVGIIRSGKIVALDA
                     PEKLKAAIDRLHRIEVSFDREIPADALARLPGVVSADRTGDKWQITTENHDAVIRLLV
                     QFSREQGAAIVTLNTLAPSLDDAFLRLTREASP"
     gene            7787..8542
                     /locus_tag="PK154_04340"
     CDS             7787..8542
                     /locus_tag="PK154_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10325.1"
                     /translation="MNPAGFPEQVRRAWAIAKKDICIYYIKGPVLIFGIFMPVFLFLA
                     FLMGSRQLPLALLVSGLVGMALFFTATAVSPAIFPWEGQAKTLERLASCPITVEAIVF
                     GDMIASTLFGIGITLITVIIGLVLGLSLLHAVTLGAGILLAACCFSAIGMLLAVPPTN
                     TPSNIMMLSSLIKFPLVFISGIFIPLEQMPPWGMALAVCSPLTYFTDLVRYSFTGTNY
                     FPVPGDIAALGMFTVVFTAGTMFLHKRTMPRRM"
     gene            complement(8708..8875)
                     /locus_tag="PK154_04345"
     CDS             complement(8708..8875)
                     /locus_tag="PK154_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10326.1"
                     /translation="MHQHEVERCESLSVSGYTKHSDKHDAKHGVRSEGEQAMRSERSE
                     RAWLSSGPGAI"
     gene            9276..10373
                     /gene="rsgA"
                     /locus_tag="PK154_04350"
     CDS             9276..10373
                     /gene="rsgA"
                     /locus_tag="PK154_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW10327.1"
                     /translation="MNKSDSGCGEAHSYTLKQLGWTEEHTQAFLKYTGPYVPGRVVCR
                     QKTVWDVFIDGGSVTAGISGALRKVGRFPAVGDFIVLLHQPEAGSSTIVDILPQKSVF
                     TRGASGKEGTEQVIAANIDIVFIVTAAGHDLNARRIERYLTIAHASGAKPVIVINKSD
                     LADDPAVLTDEIIPVASGIPVIPVSAISGYGISRLEPFLTFGTTIVLIGSSGVGKSTL
                     INRLLDRPVQETSIIRDYDGKGRHTTTVRQLFVMKSGALMIDNPGLREVGIGTASAGI
                     AETFPDMLKLATRCRFSDCQHEQEPGCAVLEAVRNGTLSLSRLENYHRLVRELTFEHE
                     KAEIGLVRSERKRWKEITKFAKEIKKGKGDF"
BASE COUNT         2216 a         2932 c         2953 g         2272 t
ORIGIN      
        1 cccggttcac ccccgagacg agcacgagtg agatgatgat gaccggaagg gcggtcgcga
       61 tgccaaagac cgccgggacg aagatcgcat ccccggtctt caatgcaatc gggatgagca
      121 tcccgaagaa caggaccgcg gcgaacgggc agagggcaag ggcgaagatg atcccgagaa
      181 ggaagccccc aaggtatccc ttttccccaa gatagagctc gatcttctgc agccatccct
      241 ggcctccgat ggaggggatg tccacaagct ccagcatcag gatccccatg atgacaagga
      301 agggcccgat cagtttctcc ccgtattcct ggaggaagag agagatagac tggatattca
      361 gtcctgcata gacgatcagg gcggtgaggc cgatataggc aaccatccgt cccagggcat
      421 agagggtccc tacgaggagc gtgtgtctgc tgtccccaat cttctttgag atgaacgcaa
      481 tggccgtgat gtttgtggcg agagggcagg gggagaaggt catcaccagt ccgatgaaga
      541 acgctgcgac cagggggatg ccactggtcc ccagcgcctc catgctgccc atgaaatcga
      601 tcaggagaag tcacctgcca ttcgcttctc gatcacacct ttgaggtagg ccagatacgc
      661 ctccttgtcc ccgatcttgt accagacatt gacattctcc tctttatgga acccgtcctt
      721 gtcgtagaca ccgatccaga gcgaggatcc tgtcggcccg tactggtcca ccagcgcctt
      781 gttttccggt tcttcgatat tgatgtggcc gaataccacc ctcccggatt ccagttccgg
      841 ggcgaagtag gtcttcacgg tatcctcggc atacgcccca acggttcggc aggagtagca
      901 ttgctgggtg cggtggaagt ggtagacttc cactttctgg acagccccgg gggttgtcat
      961 tatctggctt tcgttcgtcg cggccggagc ggtgcatcct gctccaaggg ctgccagcgt
     1021 tacgagcagc aggagtattc ccaggactac aattcgtcgc ttcataatct cattcatcat
     1081 tcttacctcc actcttcctg atcgtccgga cgactgcctg gcagagacat tcgatctcat
     1141 cgtagcgcgg ctcttccatc cctcgcttgg tgatcccttc tctcgtcgcg atgagatagg
     1201 catccgggat cttcccgatc aggtccatcc tcttctttgc acagcattcc tcgcagccgt
     1261 ccactgcgat gaggcattcg tccccggtca attccttctc cagggaccgc gtgagggaag
     1321 tgagcctcac atgacgggaa acaaggacgg gatgacggca tcggagcgag ttccctgcca
     1381 tcgtggtgag ctgcccggta tgcgagatcc cggagcaggt caggaggagg accggccggt
     1441 catccaggtc caggctggat cccctgacga agtctggctc agccacagtg gttcagccct
     1501 tcagtttctg gtagagagcg gggaggcgtc tccggtacat ctccctgatc gccggttcat
     1561 cctcgccgag aggccgaacc gggacgaggt ccatgacctg ctcgggggtg agatgggacc
     1621 acatcatggt ctgcacggtc ccttccgcca tcttccggac tgcatcggca gcccgttccg
     1681 gggagagccc gaacgagacc gcgatctgct cgagttcata gagctggaac cagaggcaag
     1741 tcggccccat ggcggagatc accgcgtagg cctccaggtc ctgctcgggg acatcgatgt
     1801 accggccgag ggattccagg agggttccca ccatcgcacg gtcctccatt gcgatccccc
     1861 cggggaaggt aaccggggta tatccgctca gtaccatgga cggggcattc gggatcatcc
     1921 tgacgatccg gtcgaatccc ccgagcatcc cggagatctg ggcgatggtc agcttgggca
     1981 tgagcgagat gacgatggca tcgggtttga gtgccggggc aatcacaccg agcaccttcc
     2041 ctgcgtccgg gggatggact gcgatgatga caaggtcctg gccggctgca agggcgttct
     2101 ggtcctgtag tacggtcacc cctggaaatt ctgcggttat ctggtcaagt gcccctctgt
     2161 tggggtcgct gacaatgatg ttctccggga gaaggcccgc ctccctccat ccatgcagga
     2221 ggatgcggac cacccgccct cccccgatac atccgattga ttcgttcatg acaggttcac
     2281 tcgcatttca tatccgggcc gcaatcgcag ccacagctgc acggggtctc ccacgattca
     2341 acgaccccga taatcacccg cttcagcaga tcggggggga ttgcctcgta gcgttcctca
     2401 ccgacatcga tcgcacggca ctctgcttca tcctgtcccg tgatgcaggc acaggagcag
     2461 cagggggtct gtaccacccg ggcatccgac aggtactcct ccaggggaac accgttgatc
     2521 aggacctggt ttgactcttc aatctgtgag tcggggagta ttgtttccct gaatcgggcg
     2581 gtaatattct tctgtctgaa atagggctgg aggtcttcga gcacgtccat tatggccccg
     2641 ccggtcaggc tgcagcgttc gcaggtgccg gtgacggtgg tgccgatgtg tttccattca
     2701 atgataagat tgcgtttcat ctgaaccatc cttgtgtccg gagacaaatt tttaccagga
     2761 ggagcatggt cgggacttcg atcaggactc ctactacggt cgcgagggct gcccctgaag
     2821 cgatcccgaa gaggatcgtg gccgtggcga tggcgacctc gaagtggttc gaggccccga
     2881 tcatcgccga gggtgcggca tcctcatagg tgaggtggat tttcttcgcg aggaaatacc
     2941 cgatcgtgaa gatgagcatc gtctggagga agagggggat tgcgatccag acgatcgtca
     3001 gcggctggga gaggattacc tcccctttga aggtgaagag gaggaccagt gtcgaaagta
     3061 aggcgatgat ggtgactgga gtcaggtagt ggacgaactt ccggttgaac cactcctcgc
     3121 ccttcgccct aatgatcagt cttctcgaaa tatacccggc tacaagcggg agagctacat
     3181 agaccgctat cgagagggcg agtgcctgcc aggggaccgg gagctggccg atcccgagca
     3241 ggaagccccc gagcggtccg tacaagagga gcatggtgag cgagttgatg gctaccatca
     3301 cgagggtatg cccgtcattg cccttcgcca ggtatcccca taccaggacc atggcggtgc
     3361 acggagcgat cccaagaagg atgcacccgg ctaggtagct ccgccagagg gggatttcca
     3421 gcatcttgat gccatccacc atgaccacgg tcccgtcccc gtagggcgct ccaaccggga
     3481 ggtccaggcc gaagggcatc ttcacgaggt caactgcctc cggcccgatg aacccgatga
     3541 agacatagcc gaggaagaag agggcgatgg catacatggt gaagggcttg atagcccaat
     3601 tgacaaacag ggtgagggcg actggcttga tgttcttccc agccttcagg acttccgcaa
     3661 agtcgatctt caccatgatg gggtacatca tgaagaagag ggcgatggcg atggggaggg
     3721 agatgatcgg ggcttccccc gagtagatgg caagggaatc gagataccgg gcaagctccg
     3781 gggcgtagtt cccaagcagg atgccgatcc cgatgcagag gaggacccat accgtcaggt
     3841 accgttcgaa ccagccgagg ccttttggtt ccttcgtgac gcaggactca ccgggcatgg
     3901 gtctccccgg gtcttcccct tccttcccag gcagcagaga cgacgagttc cacctcgtct
     3961 tcggtgaagt tcttctcgta caccttgtcg attccgagac cggtgatcac gatatgctgg
     4021 gccggggtca ctccctgggc attggcaatc ttcgccccgc atgtcatggg gcagccatcg
     4081 atgagcacca cctcatcggc ctcagcaata cgctttttca gacccggatc cccggatccc
     4141 aggagtgcga tgcacgtcgc cacgccatac ccctcgagag cgagctggag ggcggccacg
     4201 ttggagataa gaccgacgtt cgctgtccct gcacagggaa agatgatccg tctcttccca
     4261 ccagcgagtg ccgaggtgtc accgcatgca cactccggtc ttgatgccat ggcttatccc
     4321 ttcaggatct ccttgagttc tttcacatcc gcgacccggc ctgagacttt catctcccca
     4381 tcgactgcaa gcgccggggt gagcatgact ccgcggttga tgatctcgct gatgtcgtcg
     4441 accttcacta tctctgcagt gatgccgagt tcttttacgg caatctcaac gttcttgaac
     4501 agccgcttgc atttcatgca gccggtgccg agcacttcta tcttcatatg gttcctccac
     4561 ccttgcactt ctgatattcc tgtaaaagcg cgatccggta tttctcttct gctgatgcag
     4621 ggatataatt gtagatcttc accctcttca ggagttctct cccagtttcc ttttcgtccg
     4681 gaagttccat cgccttcact tctgtgagga tatggtcgag catgctgatc ccgaccacga
     4741 tgcccccgac atcgacctgc cggatacgac ggagagcttc cgcctcgcag cagggcttct
     4801 catccactgg tgccacatcc cgggatcccg aggtacggga agtagctgcc gaacacgaac
     4861 ccggcgaagg tcgagaagcc gaccaccagg agagcataga caagggtctt ttttcctccc
     4921 atgatgcggt acaagacgag cagacttggg aggctgacgg caggtcctgc caggaggagg
     4981 gatagggccg gaccgccagc catgaccccc gaggtgtatc cgaacgtgcc accgatgatg
     5041 gggacctcga ggagcgtggg catatagagg agggcgccga ccactgatgc caggaggcag
     5101 gccccgagcg agttgtcacc gaagaacggt ttgaaggtct ctggcgggag gaagaaggca
     5161 atgatgccca ctatgaatgt cccggcaatg aggatcggga agatcttctt ggtgaggtcc
     5221 cagatctcgt atccccactc ggttacttcg tcgcgctcga agtagtagat gagcagcagg
     5281 gcgactccca gggtcaggac atacacgatc gagagacgga cagagaatgg aatgagcccg
     5341 gaagctccga tcaccaggat gagcacaagg aagacaaaga agagaggtac cacccatttc
     5401 ggccgggtgg attcttcgct gccggccaca aaccccttgc ccgctgcggt cacggcctca
     5461 tcgtaagacc ggaagatggt ggccatgagg aggccgatgg caatcgacat ggtgatggcg
     5521 aaaaatgccc gggcaaaccc gatgtcccat ccaagaacgc tggcagtata gatgatggcg
     5581 aggatgttga tggcaggccc tgcatagagg aaggtggtgg caggaccgat cccgctccct
     5641 ttcttcagga tgccggcaaa catcggcagg atggtgcagg agcagacggc aaggacgact
     5701 ccggagacag atgcaacccc gtaactgacg gactttttgg catcagggct gaagtacttc
     5761 aggatcgcct ctttcttaac aaagaccgcg atcgcacctg cgataaagaa ggcagggacg
     5821 aggcaggtga tgacgtgggc agcaagatac tcgatgactg cgttcagccc ggagagcagt
     5881 gctccggtga tggcatcggt catggttgtt cacctccttc agcaggttcg aggaacaggg
     5941 gatggtggag atgcattcat atccttctcg acaaaatatt tcaactataa ttgaaatatt
     6001 gtgagacata aaacttctga taggaatcat tcagaaggaa caaccggaca accaggtgcg
     6061 gatgaacagt ttatgagatt agagtaattt atagtaattt gaaatgtcca ggcaggtatc
     6121 acgctcccga atggctcgaa aggatgtcga tgacctctcc attccccagg agattgccga
     6181 ctcgctctgt tcctgtggcg gagtggaggg actcatcgac cagatccctt ccgaccaaat
     6241 gtttgaacag atgcaggggt ggtacaaggc ctgtgccgat ccggtccgtt tgaagatcct
     6301 ctcccttctc atggtccagc cgctctgcgt ctgcgtgatc aaagcagtcg tcaggatggc
     6361 cgactccaaa ttgtcgtacc atctgaatat cctgaagaag gccgggatga tcgaagggga
     6421 gcaacaaggg cagtatatca tttaccagat caccccccgt gcaaaggtgt tcatggagaa
     6481 ggaggtgaaa cggtatgagt ctgaaaaagc cagagacgaa taagaattgc gggaccatga
     6541 cagccagcag tgtggatctc ctaaacgtag ctgagtgctc accggatacc cctgtgaaaa
     6601 aagatctcat caaaatgagc ggatcgaaca tcgtcctcgt ctgccttgag accggtcagg
     6661 agatccctcc ccatccggaa ccatatgccg tggtatttgt tgttctccaa ggggagggga
     6721 tcatcactgc cgggagtgcc aggcatcccg tgaaacccct gcaccttgtt tcggtgcgaa
     6781 aggatgagaa ccgggggatc cgatgcgacc agcggatggt gctccttggc atccgggatg
     6841 atgtctgagg ggatatccgg atgcctgacg caattgtggt cgctgatctg accaaggttt
     6901 tcggctctac ggtggctgtc gaccatgtct cgttcttggt gaaggaaggg gagatcttcg
     6961 gcttccttgg cccgaacggt gccggaaaga ccaccaccac caggatgctg acaggggtta
     7021 ttcctcctga ttccggttct gcaatgattc tcggccatga tatacagcgc gaaccggtac
     7081 tggccaaaca gggattcggc gtcgtgcccg agacctcgaa tgcctatacc gaccttaccg
     7141 cctggcagaa cctgatgctg atgggggaac tgtatgggat ttccccagtc cgtgtatcgg
     7201 aacgggcttc cgaacttctc acggtgctgg gcctttttga gcggaaggac cagaaggtcc
     7261 aggcctattc gaaggggatg aagcagcggc tcatccttgc gatggccctc ctccacgagc
     7321 cccggctcct cttcctggat gagcccacga gtggtctcga tgtccagagt acccagatga
     7381 tcctctccct gctccgggac ctgaaccggg cggggacgac tatcttcctg accacccaca
     7441 acatggagga ggcaaaccgg ctttgcaatc gggtcggaat catcaggagt gggaagatag
     7501 ttgcacttga cgccccggaa aaactgaagg cggcaattga ccgtctccac cggattgagg
     7561 tgagtttcga ccgggagatc ccggcagatg cacttgcccg gcttcccgga gtggtgagtg
     7621 cagaccgaac cggggacaag tggcagatca ccaccgagaa ccatgatgcg gtcatcaggc
     7681 ttctcgttca gttcagccgg gaacagggtg cagcgatcgt cacccttaat accctcgcac
     7741 cctcgcttga cgatgcattt ctccggctga cccgggaggc atcgccatga acccggcagg
     7801 attccctgaa caggtccgga gagcatgggc gattgccaag aaagacattt gtatctatta
     7861 catcaagggt ccggtgctca tcttcgggat attcatgccg gtcttcctgt tcctcgcgtt
     7921 cctgatgggg agccggcagc tcccgcttgc attgctggta tcgggactgg tcgggatggc
     7981 gctctttttc acagccacgg cagtatcccc cgctatcttt ccctgggagg ggcaggcaaa
     8041 gacccttgag cggctcgcat cgtgcccgat cacggtcgag gctattgtct tcggtgacat
     8101 gattgcctcc accctctttg gcatcgggat cacgctcatc accgttatca tcgggctagt
     8161 actcggcctc tcgctcctgc acgcggtcac gctgggtgcc ggtatcctgc tcgccgcatg
     8221 ctgcttctct gcaatcggga tgctgcttgc agtccccccc accaacaccc catcgaacat
     8281 catgatgctc tcgtcattaa tcaagttccc gctggtcttc atctcgggca tcttcattcc
     8341 tctcgaacag atgcctccct ggggaatggc actcgcagtc tgctctcccc tgacctactt
     8401 tactgacctt gtgcggtact ccttcaccgg gacgaattat ttccctgttc ccggggatat
     8461 cgcggcgctt ggcatgttca ctgttgtatt cactgcaggc acgatgtttt tgcacaagcg
     8521 gacgatgccg aggaggatgt agattcagtc tagatagtct tgcttattgg atccgccgtt
     8581 cataccggga aaccgtgatg ttttcactat ttttttgacg tcacaccatg ctcgctccgg
     8641 ttgtcgttct gctcctccat tcgtccggtg ctctctcctc cctccactct catcgtgtgc
     8701 ctgtttttca gatcgctcct ggtcctgatg acagccatgc tcgctcgctt cgttcacttc
     8761 gcatcgcctg ctccccctcg ctcctcacac catgcttcgc atcgtgcttg tcgctgtgtt
     8821 ttgtatagcc actgacgctc agtgactcgc agcgctcgac ctcgtgctga tgcattcgtt
     8881 taccggcgct gcttgggctg ctgtggccac ccaattctca ggcctctcgt cgcttccagg
     8941 gcgcactgct acccctttcg ctcctagccc tggtgggacg gcttggagga ggcaatgagt
     9001 gggatggatg cagtgggcac aatttacatt agaccttctg aatgatctca ttgaaggtat
     9061 tctgttggaa atgtcgtttg aggacaattg tttcgaccga taacaatctt ccctatcatg
     9121 acattattgt gaattgaaag gtcatgacag gaccactccc agtcttgaaa aatctcttca
     9181 catacttttt ttattttaag taataggcat tcacgaccac aaaccacttg gcaccttgcg
     9241 tgccaccttt tctgcactac gttcaggttt ctgaaatgaa caaatccgat tccggatgcg
     9301 gagaggcgca ttcctatacc ctgaaacagc ttgggtggac agaagaacat acgcaggcat
     9361 ttctaaaata taccggaccc tatgtacctg gtcgcgtggt ctgccggcaa aaaaccgtct
     9421 gggatgtttt tatcgatggg ggatctgtaa cggccgggat ctccggagcc ctgcgaaagg
     9481 tcggccgatt tcccgcagtg ggcgatttta tcgtgctgct ccaccagccg gaagctggct
     9541 cctccacgat cgttgatatc ctgccgcaga aatctgtatt cacacgggga gcttccggaa
     9601 aagaaggcac agagcaggtc attgcagcca acatcgatat tgtatttatt gtcacggcag
     9661 ccggccatga tctcaacgca cggaggatcg aacggtacct taccatcgcc catgcgtctg
     9721 gtgcaaaacc ggtaatcgtg attaataaat ccgatcttgc ggatgaccct gcagtgctca
     9781 ctgatgaaat tatcccggtc gcatccggta ttccggttat ccccgtgagt gccatcagtg
     9841 gttatgggat ctcccggctc gaaccgttcc ttaccttcgg cacaacgatc gtactcatcg
     9901 gttcatccgg tgtcggcaag tccacgttga tcaacaggct gctcgaccga cctgtccagg
     9961 aaacctccat tatccgcgac tatgacggga aaggccggca tacaacaacc gtacggcagc
    10021 tcttcgttat gaagagcggg gcactcatga tcgataatcc cgggctccgg gaggttggca
    10081 ttggcacggc atcagccggc attgccgaaa catttccgga tatgttaaaa cttgcaacca
    10141 gatgccggtt ctcggactgc cagcacgagc aggagccggg ctgtgccgtg ctggaggcag
    10201 tgaggaacgg gacactttct ttatcacgat tggaaaatta tcaccgcctc gtgcgggaac
    10261 ttaccttcga gcacgagaaa gccgagatag ggctggtacg gtctgaacga aagcggtgga
    10321 aagagatcac gaaattcgca aaggagatca aaaagggaaa gggggatttt taa
//
LOCUS       DAOOCO010000052        10067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6331_length_10067_cov_4.970935, whole genome shotgun sequence.
ACCESSION   DAOOCO010000052 DAOOCO010000000
VERSION     DAOOCO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6331_length_10067_cov_4.970935"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK154_04355"
     CDS             <1..255
                     /locus_tag="PK154_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HPW10328.1"
                     /translation="DLLVVAIGKPRYVGPSFVKEGAVVIDVGTNRIDGVLCGDVDFDR
                     VKDHTSAITPVPGGVGPMTIASLMENTFLAAKRSLCTGVW"
     gene            237..1049
                     /gene="folP"
                     /locus_tag="PK154_04360"
     CDS             237..1049
                     /gene="folP"
                     /locus_tag="PK154_04360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW10329.1"
                     /translation="MHRCVVKGIPIGEGFPVRLMGVINCSPESFFHGSYVMPGSILKR
                     AEEMVSLGADIIDIGARSTAPRSPPISVATEAERLSAALAELDGTGLPVSVDTMHCAV
                     LECALSHDIHAANDISGFANPAYARIVADAGLPALLMASENMPGDATNFRETLANLSR
                     IVNRCAEAGVSSYILDPGIGLWTSQRITALDWEICRHFEQCSQFGRPLLAAVSRKTFL
                     GEPDGRPPEMRLPASLGITALLIQKGADIIRTHDVRETAEVIRMASRMVDRA"
     gene            1046..1819
                     /gene="cofE"
                     /locus_tag="PK154_04365"
     CDS             1046..1819
                     /gene="cofE"
                     /locus_tag="PK154_04365"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPW10330.1"
                     /translation="MTSFLTVGIKTRIICEGDTLAEALVEGITLSEAGYLAEGDIIVV
                     AESAVATSEGRIVRLDTVIPGPDSLVLGAKYGIDPRLAEVVRTESDEIIGGIPGHLLC
                     MKGGTLLPNAGVDASNAPPGCVVLLPRDPDKSARDIRQELERRTGVRTGVIIADSRTH
                     AMRSGCSGVAIGCSGIPSVIDARGQPDLFGRPLEVTRKALADNIASAAEIVMGEAAES
                     TPAALIRGLGIPIGDYEGIESIAAEDCLFMGLLAGLKGK"
     gene            complement(2160..2798)
                     /gene="cofC"
                     /locus_tag="PK154_04370"
     CDS             complement(2160..2798)
                     /gene="cofC"
                     /locus_tag="PK154_04370"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HPW10331.1"
                     /translation="MSVPALIPFKPRNPKTRLSCVLELPEREKFAQMMLCDVVVAVRD
                     AGCEPFLLGTEPFFFNGLQVITHPGGLNESINEYLEKTPGPLLIIMADVALASGPAIR
                     RMVETEADMAIVPGTGGGTNAIYLSSADRFRVSYYGMSFEKHLRIAEESGLSCEVVDS
                     FLIHTDIDEREDLVELLIHGKGMSSRYLHELGFVLSADKGRVRVVRDTSGTE"
     gene            3019..4005
                     /gene="cofG"
                     /locus_tag="PK154_04375"
     CDS             3019..4005
                     /gene="cofG"
                     /locus_tag="PK154_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HPW10332.1"
                     /translation="MAPHLLTYTRNVFLPLTTVCHNRCGYCIFRTPVREGCIMSQGEV
                     ESILAAGVSAGCTEALFTFGERPGLERGFTEQIVRYGYSDILDYCYDLCLLSIEHGLL
                     PHTNAGIMTRDEMERFREVNASMGLMLETTADLPAHANSPGKNPATRIQMIEEAGSLK
                     IPFTTGLLLGIGETMEDREESLMVIRDLHRRYGHIQEVIVQNFCAKEGTVMERSSSID
                     PEEMCLVIRMAKDILPSSIAVQIPPNLADARHLIRCGVDDLGGISPVTIDFVNPEHPW
                     PALDHLKAIAGASILQERLCIYPKFIEKGWYHPRLEPLIRRLEQHIECERVA"
     gene            4002..4721
                     /locus_tag="PK154_04380"
     CDS             4002..4721
                     /locus_tag="PK154_04380"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW10333.1"
                     /translation="MKQGIPVYAGKAKSLYRTDKDGELLVVFRDDITAFDGGKKDVLS
                     EKGSLNAQVSARLFSLLGEHRIPTHFIRLIDNATMLVRELEMIPLEVIVRNIAAGSMV
                     RNYPVREGTPLDPPVIVIDYKDDSRHDPMLNDDLIIAMKLLSREELARVKDLALKVNI
                     ILSRFFEARGMILVDFKLEFGKYRGEILVGDEISMDSMRLWDAKTKGSLDKDVYRFEK
                     GDVLAVYRQVTEKVLKRGDNP"
     gene            4718..4963
                     /gene="purS"
                     /locus_tag="PK154_04385"
     CDS             4718..4963
                     /gene="purS"
                     /locus_tag="PK154_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPW10334.1"
                     /translation="MIFLAKITIGLKEGMLDPEALAIRHALGNLGFATEELRTKKLFL
                     VRFDAADAREAEEKAKGMCERLLANPVIHHFEVEVHR"
     gene            4960..5670
                     /gene="purQ"
                     /locus_tag="PK154_04390"
     CDS             4960..5670
                     /gene="purQ"
                     /locus_tag="PK154_04390"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPW10335.1"
                     /translation="MRFAVIQFGGSNCDRDAHYVLSDICGVDCDLVWYKDRLSRPYDA
                     VILPGGFSYGDYLRAGAIAARTPVMKEVTAHAEKGGLVLGICNGAQILAESGLVPGVF
                     TINAYPKFICRSVHLRVETTDSPFTSRYKSGDVLQIPIAHKEGRYVIPEDQLSELNER
                     GQIAFRFCDESGNLTPESNPNGALEHITGVLNKNRNVLAMMPHPERASEEILSSDDGK
                     KIFLSMISHIEHAKGVTC"
     gene            5670..5966
                     /locus_tag="PK154_04395"
     CDS             5670..5966
                     /locus_tag="PK154_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPW10336.1"
                     /translation="MTEEGDQEEQAILTWARNYAKKHHWVLNPDEKKLGIVIRGLARN
                     KQKYGEQYCPCRLRSGDKEKDAAIICPCIYHKEEVENDGSCHCRLYFRQDASNE"
     gene            complement(6134..7792)
                     /locus_tag="PK154_04400"
     CDS             complement(6134..7792)
                     /locus_tag="PK154_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPW10337.1"
                     /translation="MVTRFRRYWQIADILFKYQFGSFVQKLFPGTYRFRRCSECDIES
                     VTSVYQRLRMAIEELGPTYVKLGQILSTRQDLLPPEMIGELKVLQDHSAPLPFSEIRE
                     VIERECPHHKDCFLEIEETPLASASIAQVHRAKLSDGTPVVLKVQRPGIKEVIETDIL
                     ILESLAARIEYYYPHYAVYNPQGIVRDFAAQIRQELDFINDGRNADRLRKNMMDLKKI
                     RVPMIFWEQSTRNLLVMEYIEGVRIDHVDEIRGMGVDPREIADRGFYAYLQQIFEDGF
                     FHGDPHPGNLLVSRDGTITFLDFGIVGIIYPERRFYFTHLLVAMMQMDPEMMIKALEH
                     LGISIDETVRDQLRDDLYRAMLEAEGASVGQYSFINMSRGLTDTMRKNHIRMPQNLML
                     MLKVIIMVLDLGVTLDPKFNFGEKAEPYVRRLARRENFIDQYLYRASHSVLETVDGML
                     EMPRVINKTLRQLSTGTIKIDIVDSDILRLQQSLDRTSDKILIGLIVAGVVVGSSLVI
                     TVADVRIPDFVFYLAALAYVVAIIIGLYTIYHVIFGMNKKKGDF"
     gene            complement(7923..9611)
                     /locus_tag="PK154_04405"
     CDS             complement(7923..9611)
                     /locus_tag="PK154_04405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPW10338.1"
                     /translation="MEDNLRNILLVFALQNAVKHSSLPKPGTVIGMVMGKHPEFRSRA
                     REVSALLDEVLSQVAALSPAEREQQLRALAPDVAEDMSRPKVHVKELPGLEKADHGVV
                     MRFAPNPSGPLHLGHARAAVLNDAYVKRYGGKYILRIEDTDPKRVDPEAYGMVIRDIE
                     WLGITVHEVVYQSDRFDRYYRYGKELIERGGAYVCTCENEQFRQLKQKKTACPCRSLS
                     VEENLSLFDKMLAGEFYEGEASVRVRTDLDHPDPAMRDFPAFRILHQPLHPRIDAVVY
                     PLMNFTVAVDDHLLGVTHVIRGKDHIANTRRQRYIFDYFGWDVPVYRHYGRMGIEGVV
                     LSTSQMRQGIASGEYEGWDDIRLGTLRALSRRGISPEAVRQAVLDIGIGETDISFSWD
                     NLYAQNRMIVDPVANRYFFVPHPVGAAIRDAPHHVARALLHPNEPERGTRVLQFTGTV
                     LLPRQELEKHPSLIRLKDLFNVKVSYDERGYLFTYAGDQLSEAREAKAPIIQWLPADC
                     ALPCVLRTPEGDVEGVCETGVMREAGSVLQFERVGFARIDDTTGDRITAYFTHR"
     gene            complement(9614..>10067)
                     /locus_tag="PK154_04410"
     CDS             complement(9614..>10067)
                     /locus_tag="PK154_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW10339.1"
                     /translation="EKKTGVLYAASAGIGGILAGGNPSYVDALYHFGLGIGMAFQIQD
                     DIIDLLAPPAVSGKDQASDLREGKKTLIAMKALEKGFDLSEFRRDLSPAEVDAIIKKL
                     ESLGVLEEVRMSARDLIKNGKKRISILPDSEEKQLLSDLADYFLSRSY"
BASE COUNT         2393 a         2718 c         2705 g         2251 t
ORIGIN      
        1 gatctcctgg tggtcgccat cggtaaacca cgatacgtcg ggccctcgtt tgtaaaagag
       61 ggagcggtgg tgatcgatgt cggcaccaac aggatcgacg gcgttctctg cggtgacgtt
      121 gactttgacc gggtcaaaga ccacacctcc gcgatcacgc ctgtccctgg gggtgtcggg
      181 ccgatgacca ttgcatccct gatggagaac accttcctcg ctgcgaagcg gtccctatgc
      241 accggtgtgt ggtaaagggt ataccgatcg gggagggatt cccggtccgg ttgatgggag
      301 tcatcaactg cagccccgag tcattttttc atggatccta tgtcatgccg ggatccatcc
      361 tgaaacgcgc tgaggaaatg gtctctctcg gtgcggatat catcgacata ggcgcgagaa
      421 gcactgcgcc acggtctccc ccgataagtg tggcaactga agctgaacgg ctttctgctg
      481 cactcgctga gctggatggg accggtctcc cggtctctgt ggacaccatg cattgtgcag
      541 tgctcgagtg tgctctctcc catgacatcc atgccgccaa tgatatctcg ggattcgcca
      601 atccagccta cgcccgcatc gttgcagatg caggactgcc ggctctcctt atggcatcag
      661 agaacatgcc gggtgatgcg acaaatttca gagagacgct tgcaaactta tcgcggatcg
      721 ttaatcgttg tgcagaggca ggggtatcct cgtatatcct cgatccagga atagggctct
      781 ggacctcgca acggatcaca gccctggact gggaaatctg ccggcacttt gagcagtgtt
      841 cccagttcgg caggccgctc ctggcagcgg tctcccgaaa gacctttctc ggagaaccgg
      901 atggccggcc gcccgagatg cgccttccag cgtccctcgg gatcacggcc ctgctcatcc
      961 agaaaggggc agatatcatc agaacccatg atgtcaggga gaccgccgag gttatcagga
     1021 tggcatcacg gatggtggac agagcatgac cagtttcctg accgtgggaa taaagacacg
     1081 gatcatctgc gagggggata ccctggccga agcactcgtt gaagggatca ctctgtcgga
     1141 agcaggatac ctggcagaag gtgacatcat tgtggtggcg gagagtgcag tcgctacaag
     1201 cgagggaagg attgtacgcc tggatacggt aatccccggg cctgattctc ttgtcctcgg
     1261 agcgaagtat ggcatcgatc cccggcttgc tgaagtggta agaaccgaga gcgatgagat
     1321 catcggaggg atcccgggac acctgctctg catgaagggg gggaccctgc tccccaatgc
     1381 cggtgtcgat gcatccaatg ccccccccgg ttgtgtcgtg ctgctcccca gagatccgga
     1441 caagagtgcc cgtgatatca ggcaggaact ggagcgcagg acaggggtgc ggacaggggt
     1501 catcattgca gactcccgga ctcatgcgat gcgatcaggg tgcagcgggg ttgctatcgg
     1561 ttgttcgggt atcccttcgg tgatcgatgc acggggacag cctgacctct ttggaagacc
     1621 ccttgaagtc accaggaaag cacttgcgga caatatcgcc tctgctgccg agatcgtgat
     1681 gggagaggcc gcagagtcaa caccggcagc ccttatccgc ggactgggga tccctatcgg
     1741 tgactatgaa gggatcgaat cgattgccgc cgaagactgt ctctttatgg gcctgctggc
     1801 cggtctgaag gggaagtaac gggaccccgg gacaggaaga aggagagtgc ccgatccaac
     1861 ctttttccat gaaaatcact tcataatttt cataatgtat gcccgtatcc cgaaatcggg
     1921 gctcatagga ggtttatatg aaaatgcctc cggcattttc attctctatg cttgtgtcac
     1981 gaagggaatc attcagtttt tttaatttta cctcaccagt tctgaacatc agggagattt
     2041 atggggattc gccatagaga gagtcatacg ctgtttctca ttgagagcgc ttgtcgttgt
     2101 cccggaacat cgaagggcga agacggacag ccggagaaca agtgaggaga gccccccgct
     2161 cattccgttc cggaggtgtc ccttactacc ctgacccttc ccttgtcagc ggagaggacg
     2221 aagccgagct cgtgcaggta ccggctgctc attccctttc catggatgag cagctccacg
     2281 aggtcttcgc gctcatcaat atcagtatgg atgagaaaag aatccaccac ctcgcaggag
     2341 agcccacttt cctctgcgat ccggagatgt ttttcaaagc tcattccata ataactgacc
     2401 cgaaagcggt ctgcggagga gagatagatg gcatttgtac ctccccccgt tcccggcacg
     2461 atggccatgt ctgcttctgt ctcaaccatc cttcgaatgg cagggcctga agcgagggcg
     2521 acgtcagcca tgatgatcag gaggggtccg ggagtctttt ccaggtactc attgatcgac
     2581 tcgttcaacc cgccaggatg ggtgatcacc tgcagaccat tgaagaagaa cggttctgta
     2641 ccaaggagaa atggttcgca tcctgcgtcc cgtaccgcca ccacgacatc gcacagcatc
     2701 atctgggcaa atttttccct ctcggggagt tcaaggacgc aggagagcct ggtcttggga
     2761 ttccttggtt tgaaagggat cagagcaggg actgacattt cactacaaca ctacgtgcag
     2821 gccaaataaa taaatcacgc atcaaggaaa aggcgctccc tgccggccat cctgagagta
     2881 attgatgaga gaacgttgat accgttcctc tcatattttt tcttcagaga atggtatcct
     2941 ggcatgttcc aacaccaggg ccattcccat gccggaggaa tttttatccc gaaacgccca
     3001 gatcaactac catatcacat ggctcctcac cttctgacct acacacgaaa cgtctttctc
     3061 ccgctcacca cggtctgcca taaccggtgc ggatactgca tcttccggac accggtccgg
     3121 gagggatgca ttatgagcca gggcgaggtg gaatcaattc ttgctgccgg agtatccgca
     3181 gggtgtaccg aggctctctt cacgttcggc gaacgccctg gtttggaacg gggattcacg
     3241 gaacagatcg tgcgatatgg ttattctgat atcctcgatt attgctatga cctgtgcctg
     3301 ctgagtattg agcacggtct tcttccccat acgaatgccg gtatcatgac gagagatgag
     3361 atggagcggt tccgcgaggt gaatgccagc atgggcctca tgctggagac tacggctgat
     3421 ctgccggcgc atgccaactc ccccggaaaa aatcccgcta cccggataca gatgatcgaa
     3481 gaggccggaa gcctgaagat cccgttcaca accgggcttc tccttggcat cggtgagacg
     3541 atggaagaca gggaagaatc cctgatggtg atcagggacc ttcaccggag atatggtcac
     3601 atacaggaag taattgtcca gaacttctgt gcaaaagaag gaacggtgat ggagcgatcc
     3661 agcagtatcg acccggaaga gatgtgcctt gtcatacgga tggcaaaaga tatcctccct
     3721 tcgagcatcg cggtccagat cccgcctaat cttgccgatg cacggcacct catccggtgt
     3781 ggcgttgacg acctcggagg aatctccccg gtaaccatcg acttcgtcaa tcccgagcac
     3841 ccatggcctg cacttgatca tctgaaagct attgccggag catcgatctt gcaagagcgc
     3901 ctctgcatct atccgaaatt tattgaaaag ggatggtacc acccgcggct ggaacccctt
     3961 attaggagac tggaacaaca tatagaatgc gagcgtgtag cgtgaaacag ggtatcccgg
     4021 tctatgcagg aaaggcaaag tcattgtatc gcaccgataa ggacggtgag ctccttgtcg
     4081 tgttcaggga tgacatcacc gcgtttgatg ggggaaagaa agatgtcctt tcagagaaag
     4141 gaagtctgaa cgcacaggta tcggcacggc tcttctccct ccttggggaa caccgtatcc
     4201 ccacccactt catccgcctt attgacaatg caaccatgct tgtccgggaa cttgagatga
     4261 tcccgctcga ggtgattgtg agaaatattg cggcaggatc catggtcaga aactaccccg
     4321 tcagggaagg gaccccgctt gacccccccg tcatcgtgat cgattacaag gacgattccc
     4381 gccatgatcc catgctcaac gatgacctca ttatcgcgat gaaactcctg tcgcgagagg
     4441 agctcgcccg ggtgaaagac ctcgcgctca aggtcaatat catcctctcc cggttctttg
     4501 aggccagggg gatgatcctt gtcgatttca agctggaatt cgggaaatac cggggggaga
     4561 tcctcgtcgg agacgagatc agcatggact cgatgcgcct ctgggatgcg aaaacaaagg
     4621 gatcccttga caaagatgtg taccggttcg aaaaaggtga tgtgcttgcc gtctaccggc
     4681 aggtgactga aaaagtcctg aagagaggag ataatccatg atattccttg caaaaatcac
     4741 gatcggcctg aaagaaggca tgcttgaccc ggaagcactt gctatccggc atgccctcgg
     4801 aaaccttgga tttgcgaccg aggaactccg gacaaaaaaa ctcttcctcg tccgttttga
     4861 tgccgctgat gccagagaag cggaagagaa agcaaagggg atgtgtgagc ggctgctcgc
     4921 aaatcccgtc atccaccatt tcgaggtaga ggtccaccga tgaggtttgc tgtcatccag
     4981 ttcgggggca gcaactgtga tcgcgacgcc cactatgtcc tctcagatat ctgcggggtg
     5041 gactgtgacc ttgtgtggta caaggatcgc ctctcccgac cgtatgatgc ggtgatcctg
     5101 ccgggaggtt tctcgtacgg tgattatctg cgagccgggg ccatcgctgc ccggacccct
     5161 gtcatgaagg aagtgaccgc tcacgcagag aaagggggcc tggtccttgg catctgcaac
     5221 ggggctcaga tccttgccga gagcgggctc gtccccgggg tgttcaccat caatgcgtac
     5281 cctaagttca tctgcaggag tgtccacctc agggtcgaga caacagattc gccgttcacc
     5341 tcacgctaca agagcggtga cgtgctgcaa atccccatcg ctcacaagga gggaaggtac
     5401 gtgattcctg aagatcagct atccgagttg aacgaacggg ggcagatcgc attcagattc
     5461 tgtgatgagt caggaaatct cacccctgag agcaacccga acggggctct tgagcacatc
     5521 accggcgttc tcaacaagaa ccgcaatgtc cttgccatga tgccgcatcc tgaacgggct
     5581 tcagaggaga tcctctcttc agatgacggg aagaagatct ttttatcaat gatctcccat
     5641 atcgagcatg cgaaaggagt gacctgctga tgacagaaga aggggatcag gaggagcaag
     5701 cgatccttac ctgggccagg aattatgcaa agaaacatca ctgggttctt aatcctgatg
     5761 agaaaaaact cggcattgtg atcaggggtc ttgcccggaa caagcaaaaa tacggagagc
     5821 agtactgccc ctgcaggctt aggtcagggg ataaggagaa ggatgcagcg atcatatgcc
     5881 cctgtatcta tcacaaggaa gaagtggaga atgacggaag ctgccactgc aggctgtatt
     5941 tccgccaaga cgcatctaac gaatgatttg acttcacatc atgttgtttg ggtcccatca
     6001 ccctgaagca gtaatgttca ggaaagaatc ggtgataaag gcaggatcta tcgaaccggg
     6061 gaccatacca agattacgag ggggtacctc ccggggcata ccattgcttt caggacacat
     6121 gatgcactga tgcttaaaaa tcaccttttt ttttgttcat gccaaatatt acgtggtaaa
     6181 tcgtataaag accaataata atcgcaacca cgtaggcaag ggctgcgaga tagaagacaa
     6241 agtcaggtat cctgacatca gccaccgtta tcaccagtga cgacccgacc accacaccgg
     6301 caacgatcag tccgatcagg atcttgtcac tcgtccggtc aagcgactgc tggagccgga
     6361 ggatatccga atcaacaata tcgatcttga tggtgccggt ggagagctgc cggagtgttt
     6421 tgttgatgac ccggggcatc tcgagcatac catcaactgt ctcgaggacc gagtgacttg
     6481 cccggtacag atactggtcg atgaagttct cccgccgggc aagcctcctg acatacggtt
     6541 ctgccttctc cccgaagttg aacttcgggt cgagcgtaac accaagatcg agcaccatga
     6601 tgattacctt gagcatgagc atcaggttct gcggcatcct gatatggttt ttcctcatgg
     6661 tatcggtcag tcccctgctc atgttgatga aactgtattg gccgactgac gctccttcgg
     6721 cttccagcat cgcacggtag agatcgtccc tgagctgatc acgcacggtc tcatcgatgg
     6781 aaataccaag atgctcaagt gccttgatca tcatctcggg atccatctgc atcatcgcga
     6841 caaggagatg ggtgaaatag aaccggcgtt cgggatagat gatcccgaca atcccgaaat
     6901 ccaggaaggt tatcgtccca tcccttgaca cgagaagatt tccggggtgg ggatcaccat
     6961 gaaaaaaacc atcctcaaag atctgttgca aatatgcata gaatccgcgg tctgcaatct
     7021 cccttggatc cacccccata ccacgaatct catctacatg gtcgattcgc accccctcaa
     7081 tatactccat gacgaggaga tttcgggtac tctgctccca gaagatcatg gggactctga
     7141 tctttttgag atccatcata ttctttcgga ggcgatccgc attccgccca tcattgataa
     7201 aatcgagttc ctgccggatc tgggctgcga aatcccggac aatcccctgt ggattgtaaa
     7261 cagcatagtg agggtaataa tactcgattc ttgcagccag agactcaagg ataagaatat
     7321 cggtctcaat cacctctttt atgcccggac gctgcacttt caggacaacc ggggtaccat
     7381 cagagagctt cgcccgatgc acctgtgcga tggaagcaga tgccagaggt gtttcttcaa
     7441 tctcaagaaa gcaatcctta tggtgtgggc actccctctc aatcacttcc ctgatctcgc
     7501 tgaatggcag aggtgcacta tggtcctgaa ggactttcaa ctccccaatc atttctggtg
     7561 gaagaaggtc ttgcctggtc gagaggatct ggccaagttt tacgtaggtg gggcctagct
     7621 cctcgattgc catccgcaac cgctggtaga ccgaggtgac agattcgatg tcacactcac
     7681 tgcacctcct gaaccggtag gtcccgggaa agagtttctg gacaaaagag ccgaactgat
     7741 acttgaagag gatatcagcg atctgccagt atctcctgaa tcttgtcacc atccggttca
     7801 cttcacctgt attgaatagt accttggaaa gatattattc cctttctctt catgatggca
     7861 gatgaaaggt agtgggaaaa aagttcagga tgcctggtaa cagggaaaaa gagattcctc
     7921 acttaccggt gggtgaaata cgccgttatc ctgtcgccgg tagtatcatc gatccgggca
     7981 aatcccaccc gttcaaactg tagtaccgat cctgcctctc tcatcacacc cgtctcgcag
     8041 accccctcaa catcgccttc tggggttcgg agcacacaag gcaatgcgca atctgccggc
     8101 aaccactgga tgataggggc cttcgcttcc cgggcttccg ataactgatc cccggcatag
     8161 gtgaacaggt atccccgttc atcgtaagat accttgacat tgaagagatc ttttaagcgt
     8221 atcagagacg gatgtttctc aagctcctgc cgcgggagca gaacggttcc cgtgaactgc
     8281 aggaccctgg tgcctctctc aggttcgttc gggtgcagga gagcccgggc aacgtgatgc
     8341 ggtgcatccc tgatagcagc gccaacaggg tggggaacaa agaaataccg gtttgctacc
     8401 ggatctacga tcatccggtt ctgggcatag aggttgtccc aggaaaagga gatatctgtc
     8461 tccccgattc ctatatcgag gactgcctgg cggaccgctt caggggagat acccctccgg
     8521 gacagcgccc tgagtgtgcc gagccggatg tcatcccacc cctcgtattc acctgatgca
     8581 attccctgtc gcatctggga tgtggagaga acaacgccct cgatccccat ccgcccatag
     8641 tgacgatata ccgggacgtc ccacccaaaa taatcgaaaa tatatcgttg tctccgtgtg
     8701 tttgcaatat gatccttgcc acgaataaca tgcgttaccc ccaggagatg atcatccacc
     8761 gcaacagtga agttcatcag gggatagacc accgcgtcga ttcggggatg aaggggctgg
     8821 tgaaggatgc ggaacgccgg aaagtcccgc atcgccggat ccggatggtc gagatcggtc
     8881 cggactctga ccgaagcttc tccttcgtag aactcccccg ccagcatctt atcaaagagt
     8941 gagagattct cctcgactga gagcgaacgg cagggacagg cagtcttctt ctgtttcagc
     9001 tgcctgaact gctcattttc acaggtacag acataggctc ccccgcgctc gatcagttcc
     9061 tttccgtagc ggtaatagcg atcaaaacga tcgctctggt aaacgacctc atggacggtg
     9121 atcccaagcc attcgatatc cctgatcacc atcccatacg cttcgggatc aaccctcttg
     9181 ggatcggtat cttcaatccg gaggatgtat tttccgccgt atcgttttac atacgcatcg
     9241 ttgagaacgg cagctcgtgc atgccccagg tgcagcggcc cgctgggatt cggagcaaac
     9301 cgcatcacga caccatggtc tgccttctca agaccgggga gctcctttac atgtactttg
     9361 ggccgggaca tatcttcggc aacatcaggg gccagggcac ggagctgttg ctcccgttcg
     9421 gccggcgaga gagccgcaac ctgagaaagc acctcgtcca ggagcgcaga gacttccctg
     9481 gccctcgagc ggaattcggg atgcttcccc atgaccatgc ctatcaccgt ccccggcttg
     9541 ggcagactcg aatgtttcac cgcattctgg agagcaaaaa ccagcaggat attccggaga
     9601 ttgtcttcca tgttcaataa ctccgggaaa gaaaataatc ggccagatcg gagaggagtt
     9661 gtttctcttc cgaatcaggg agaatgctga tacgtttctt cccgttttta atcagatcac
     9721 gggctgacat cctgacctct tcgagaacac caagggattc aagttttttt atgatggcat
     9781 caacctctgc aggggagagg tcgcggcgga actctgacag gtcaaaccct ttctccaggg
     9841 ccttcatcgc gataagggtc tttttccctt cccgaagatc tgaagcctgg tcctttccgc
     9901 tcacggcagg gggtgcgagg agatcgatga tatcatcctg gatctggaag gccatcccga
     9961 taccaagccc gaagtggtat aaagcatcga catacgaagg atttccaccg gccaggattc
    10021 cccctattcc tgctgatgct gcatagagga cgcctgtctt cttctcc
//
LOCUS       DAOOCO010000053        10003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6396_length_10003_cov_5.121934, whole genome shotgun sequence.
ACCESSION   DAOOCO010000053 DAOOCO010000000
VERSION     DAOOCO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6396_length_10003_cov_5.121934"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PK154_04415"
     CDS             complement(<1..409)
                     /locus_tag="PK154_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPW10340.1"
                     /translation="MHSDAVDTAHVLVIGSGGAGVRAAIEAAEYGETMLVSKTLTGKG
                     GCTTMAEGGYNAVLREADACEIHYEDTLKGGAYLNDPELVQILVHEAPQRIRDLLEWG
                     AVFDVTDSCEVAQRPFGGQRFPRTCYAGDRTGHE"
     gene            482..1870
                     /locus_tag="PK154_04420"
     CDS             482..1870
                     /locus_tag="PK154_04420"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPW10341.1"
                     /translation="MKFGGTSVADAATVRKVVDIVEGYRKEGHELAVVVSAQRGVTDQ
                     LIATATEIAGSRNAPAIDPLISSLRLRHKSVLAEVGSDFEEEVSGEMEERLCELGNML
                     HAVHNLRELTPRSLDYIITYGERLNSLVVSAALRQRGIPSRVMDGCEAGILTTTQHGE
                     AMALPESQERIRGRVQPLLTGTVPVIMGFMGCTEKGIITTLGRSGSDYSAAIIGAGID
                     AGEIWIWTDVDGIMTSDPRLISDARVIPSLSYLEVMELSFFGAKVMHPRSIEPAMKKN
                     IIVRVKNTFNPSHPGTAIVRGEKRDSRVVKALTYIDKVALINICGAQMIGRPGVARAI
                     FSALAEKEVNVMMISQGSSEANISLIIDESQLMPATEALASIVKEGLVREVTSNRDVC
                     AVAVVGSGMAGAKGTGGRIFTALGNGGVNVMMISQGSSEVNISFVVKQADGPKAVRIL
                     HDEFRLSEEDYA"
     gene            1863..2858
                     /gene="purM"
                     /locus_tag="PK154_04425"
     CDS             1863..2858
                     /gene="purM"
                     /locus_tag="PK154_04425"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPW10342.1"
                     /translation="MPESFSYRDAGVDIDQEAEGIRALIAALTFRRKGRFSMMGSVGH
                     FAGLIDFGEMSLALTVDGVGTKMLVADRAQDWSTVGIDCIAMNVNDLYVMNMEPVAFV
                     DYIATDSISMEKMAQIGRGLNEGARLANIDIVGGETATLKNLVSGLDLAGTCLGVQKK
                     GEIITGDRVVPGDLIIGIPSSGIHSNGLTLARRIVDSRDAWEERLSNGKTLCQELLCP
                     TRIYAEVLPVCRKTVVTGMCHITGGGLHNLSRITRYGFGIDNPIEPPEIFQWLREAGS
                     VSDREMYRTFNMGMGYTLIAPESSLPAITALIPDARPVGTITEQPGIRLSGKKFT"
     gene            complement(2864..3409)
                     /locus_tag="PK154_04430"
     CDS             complement(2864..3409)
                     /locus_tag="PK154_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HPW10343.1"
                     /translation="MRTVVPGPCPVCQTEIPYLYQTDEIPYFSEILIISALCPSCGYR
                     LADTQLLKHTEPSRWELIIESPDDMMIRVVRSMTGTISIPELGVRIDPGPACEGFVSN
                     VEGVLNRIGKVLQSLLSWAETADERERIHSLQGSLEMVREGRLTATLVIEDLSGNSAI
                     IADKAKVCRIEVIEDDLEELS"
     gene            complement(3410..3781)
                     /gene="sepF"
                     /locus_tag="PK154_04435"
     CDS             complement(3410..3781)
                     /gene="sepF"
                     /locus_tag="PK154_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPW10344.1"
                     /translation="MVKFLDSLLGKSTPSTEDDYMDLDLEAFEQAEASAPAMFVKIAA
                     IGDIKDSPRIKDEIYNGNVVIADISRLKMDKVMYERVLKDLKEVAKDVNGDIIGLGDQ
                     RYVVITPNSVKISRDKIGGVE"
     gene            complement(3877..4374)
                     /locus_tag="PK154_04440"
     CDS             complement(3877..4374)
                     /locus_tag="PK154_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW10345.1"
                     /translation="MAKITGRKRHSIRKSQASALFSGLCAQIGESASLFMSDRVEILE
                     TNAGFALYLIDKKPQLMDSGEWVFPTLQGLLEHPFPERRVVVDSGAVPFVVNGADIMR
                     PGIVSVSEDVKAGCPVQVVEERHGKPLALGVALYNAGDLMGKTSGKMVKTLHYIGDDL
                     WNVEF"
     gene            4550..4777
                     /locus_tag="PK154_04445"
     CDS             4550..4777
                     /locus_tag="PK154_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW10346.1"
                     /translation="MTKRPLDILDQVLNRQPVIVSLKGGRELRGVLQGYDVHMNLVLD
                     KAEEVEGGQARSVGTLIVRGDNVIYISPSMD"
     gene            4796..4978
                     /locus_tag="PK154_04450"
     CDS             4796..4978
                     /locus_tag="PK154_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HPW10347.1"
                     /translation="MSKGTPSMGKRSGKVHIACRRCGSISYHLRHRTCSACGFGKSTR
                     MRKYNWINKRPKIPTH"
     gene            4988..6406
                     /gene="purF"
                     /locus_tag="PK154_04455"
     CDS             4988..6406
                     /gene="purF"
                     /locus_tag="PK154_04455"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW10348.1"
                     /translation="MCGIVGIRDAGGVSFSLYYALFALQHRGQESAGISTFDGVQLYK
                     HKGQGLVAEVFDPALLRGLEGYVGIGHVRYPTTGANKPENAQPFNFLFRDHIISIAHN
                     GNLVNACELRDEYEARGQIFCTTTDTELIAKVITDEMSTSGCIEDAVHRCMRILKGSY
                     SVVLMMDGILYGFRDPLGIKPMCIGKTSQGIVLASESVAVDALGGTFLRDLKPGELVC
                     IDEDGMRAMQIAVSDCRAHCIFEYIYFARADSILDGALVYDVRRNIGGKLHEEAPVNA
                     DSICPVPDSGTAYAIGYSKTSHIPFVESLMKNRYMGRTFIMPSQIEREKAVRMKLNPI
                     GDHLKGKSIVLVDDSIVRGTTSRRIINIIRDAGAKEVHVRIGSPAIKAPCYLGVDIPT
                     REELIASDKNEEEVRGRITANSLHHISLEALVESIGIERKHLCTGCLTGCYPVSITGE
                     QSQPPAIDFLDGTYQTKLELFE"
     gene            complement(6440..6514)
                     /locus_tag="PK154_04460"
     tRNA            complement(6440..6514)
                     /locus_tag="PK154_04460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6477..6479),aa:Met,seq:cat)
     gene            6623..7024
                     /gene="trxA"
                     /locus_tag="PK154_04465"
     CDS             6623..7024
                     /gene="trxA"
                     /locus_tag="PK154_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW10349.1"
                     /translation="MDDELQRIREKKLREMTEHLHGAEVPLVHPLNAANFRQLLTANP
                     FLVIDFWAEWCGPCRLVAPVIEELSREFASIVTFAKCNTDENPGIAGGFGITAIPTII
                     LFRDGTAVDMVVGAYPKEALKARIKKAFHLP"
     gene            7103..7663
                     /locus_tag="PK154_04470"
     CDS             7103..7663
                     /locus_tag="PK154_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPW10350.1"
                     /translation="MRLIVGLSGASGIIYGIRMLEVLHDLKIETDLVMTGMAEKMVEI
                     ETSRSVEQVRDFATRCHDNSDFTSVLASGGYRTMGMVVLPCSMKTLAGIACGFADNLL
                     LRAADCTLKERRPLILVPRETPLNLIQLKNMVTLAESGVTILPAAPGFYHRPETIDDL
                     VDHIVGKTLDVFGVDHHLYHRWRQDF"
     gene            complement(7909..9477)
                     /locus_tag="PK154_04475"
     CDS             complement(7909..9477)
                     /locus_tag="PK154_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPW10351.1"
                     /translation="MAVSTTMVVVVTAIYVVIMLILGYIGYKKTRNTEDYLVAGRNAH
                     PVVIALSYGATFISTSAIVGFGGQAANLGMGLIWLTVFNIGVGILIAFVVFGKKTREI
                     GQRLKAITFPDLMGKIYQSQFMQVLTGVIILIGMPLYTAAVLIGGAQFIKETLGISYG
                     ISLIGFALIVAIYVVFGGLIAVMYTDAVQGAIMLVGMTFLLIATYLLLGGVTVANTAL
                     TNMADLVPVKLAEQGMNGWTVMPDLGSPIWYTVITTLVLGVGIGVLAQPQLVVRFMTA
                     KDNRSLNRAVLVGGPFILMMTGVAFTVGALTNVYFYQTQGKIAAQVATAGNIDTIIPI
                     FINSAMPEWFVVLFMVTLLAAAMSTLSSQFHAMGTALICDVWGRGRECALSLRANQAG
                     VLAMIVISVIIAFLMPGSIIAIATAMFMGLCASAFLPIFAVAVYCKRPDPLAAKASLV
                     IGALAWFIWAFFVNTKYAAVFGLSKALLGTVGVLAYPWSAIDPIVIALPLSAITILSL
                     QYLRGGCAPGAQTA"
BASE COUNT         2405 a         2611 c         2734 g         2253 t
ORIGIN      
        1 tctcatggcc ggtacggtca ccggcatagc aggtcctggg gaaccgctgc cccccgaacg
       61 gtcgctgggc aacttcacag ctgtccgtaa catcaaagac ggctccccat tccaggagat
      121 ccctgatccg ctgcggtgct tcatgaacga ggatctgaac gagttcaggg tcattgagat
      181 acgcaccacc cttcagcgta tcttcataat gtatctcaca ggcgtcggcc tccctgagga
      241 cagcattgta acccccttct gccatcgttg tgcagccccc ctttccggtg agggtctttg
      301 aaacgagcat ggtctcgccg tattctgcag cctcaatcgc tgcacgaaca ccggcaccgc
      361 cactcccaat tacgagtaca tgcgccgtat ccaccgcatc agaatgcatc ttattagtac
      421 gtgcgtcata tactaacata aatagatgca aaggactgtg tgagcccgga tgaggttttt
      481 aatgaaattt ggcggcactt cagtggccga tgcagcgacc gtcaggaagg ttgtggatat
      541 cgttgaagga taccggaagg aaggtcatga acttgcggtg gtagtatcgg cccagcgggg
      601 agttaccgac cagctcatcg ctaccgccac cgagatcgcc ggaagccgca atgctcctgc
      661 cattgatccc ctcatcagct ctctccgcct ccgtcacaaa tccgtacttg cggaggtcgg
      721 atcggatttt gaggaggagg tcagcggaga gatggaagag aggctctgtg aactcggcaa
      781 catgctgcat gccgttcata atctgagaga gctaactccc cgctcccttg actatatcat
      841 cacctacggg gaacggctca attcccttgt cgtgtctgca gcgttgagac agcggggtat
      901 cccctcgcgt gtgatggatg gatgcgaggc agggattctg acgacgaccc agcatggaga
      961 ggctatggcc cttccggaga gccaagagag gatcaggggc agggtgcagc cgctgctcac
     1021 cgggacggtc ccggtcatta tgggattcat ggggtgcacc gagaagggaa tcatcaccac
     1081 cctcggcagg agcgggtcgg actattccgc ggcgataatc ggtgcaggaa tcgatgccgg
     1141 ggagatctgg atctggaccg acgtggatgg aatcatgacc tccgatcccc gcctgatttc
     1201 agatgcaagg gtgataccat ccctctccta ccttgaggtg atggaactct cgttcttcgg
     1261 cgcaaaggtg atgcatcccc gatccatcga acccgccatg aagaagaata tcatcgtgcg
     1321 ggtcaagaac accttcaatc catctcatcc cggaacagca atagtcaggg gcgagaaacg
     1381 cgacagccgg gtggtaaaag cgctcaccta catcgacaaa gtagccctga tcaatatctg
     1441 cggggcacag atgatcggac gcccgggggt ggcaagggca atcttctctg cgctcgccga
     1501 aaaagaggtg aacgtgatga tgatctcgca gggatcgagc gaggccaata tctctctgat
     1561 cattgatgag agtcagctca tgcccgctac tgaagcactg gcatcaattg taaaagaggg
     1621 gctcgtccgg gaggtgacat ccaaccggga cgtctgtgcg gtcgccgtgg tcggttccgg
     1681 gatggcgggc gccaaaggga ctggtggccg gatattcacg gctcttggga acgggggggt
     1741 gaacgtgatg atgatctcgc agggttcatc cgaggtaaat atctcttttg tcgtgaaaca
     1801 ggcagacggc ccgaaggcag tccggatcct ccatgatgag ttccggctgt cagaggagga
     1861 ttatgcctga atccttctca taccgggatg ctggtgtgga tatcgatcag gaagcagagg
     1921 gaatcagggc actgatagca gcgctgacat tcaggaggaa gggccgtttc tcgatgatgg
     1981 gaagcgtagg gcattttgcc ggcctcatcg attttggtga aatgtctctt gcgctcaccg
     2041 tggatggcgt ggggacaaaa atgctcgttg ccgacagggc acaggactgg agtaccgtcg
     2101 gcattgattg catcgccatg aatgtgaacg acctgtatgt catgaacatg gaacccgtcg
     2161 ccttcgtaga ctatatcgcc acggattcca tatcgatgga aaaaatggcc cagattggca
     2221 gaggcttgaa cgaaggggcc cggctggcaa atatcgatat cgtgggggga gagactgcaa
     2281 ccctgaaaaa tcttgtttcc ggtctcgatc ttgcgggaac ctgtcttggc gtacagaaaa
     2341 agggggagat aatcaccggc gaccgggttg tccccgggga tctcatcatc gggattccgt
     2401 catcaggaat ccacagcaac gggctgacgc tcgcccggag gattgttgat tcccgtgatg
     2461 catgggagga gcgactctcc aacggaaaga cgctctgcca ggaactcctc tgcccaaccc
     2521 ggatctatgc agaagtgctc ccggtctgca ggaagacggt ggtgaccggc atgtgccaca
     2581 tcaccggggg cggtctgcac aatctctccc ggataacccg atatggtttc ggcatcgaca
     2641 acccgattga gccgccggaa attttccagt ggctccgcga agcaggttcg gtcagcgacc
     2701 gggagatgta ccggaccttc aacatgggta tgggctatac cctcatcgca cccgagagca
     2761 gccttccggc cattaccgcg ctgattccgg atgcccgacc cgtcggaaca ataaccgaac
     2821 agccaggtat caggctgtcg ggtaagaaat tcacgtgatg gtatcaggag agctcttcga
     2881 ggtcatcctc gatcacctca atcctgcaga cctttgcctt gtcagcaatg atggcactgt
     2941 ttcctgaaag atcctcgatg acaagtgtag ccgtgagcct gccttccctc accatctcaa
     3001 gcgatccctg aagcgagtgt atcctttcac gctcatcagc agtctctgcc catgagagga
     3061 ggctctggag gactttcccg atacgattca gtactccctc gacgtttgag acaaatccct
     3121 cacacgcggg accgggatcg atcctgaccc ccagctccgg tatcgagatg gtgccggtca
     3181 tgctccggac cacccgtatc atcatatcat cgggagattc gatgatgagt tcccagcgcg
     3241 aaggttcagt atgcttgagc agctgcgtgt cagcaagacg atagccgcag gagggacaga
     3301 gcgcagagat gatcagaatc tcggaaaaat aggggatctc atcagtctgg tacagatatg
     3361 gtatctcggt ttgacagaca gggcacggac cgggtacaac ggtccgcacc tattcgaccc
     3421 ctccgatctt gtcacgggag atcttcaccg agttcggggt gatgactaca tatctctggt
     3481 caccgagacc gatgatgtcc ccgttcacgt ccttggcaac ctcttttagg tccttcaata
     3541 ccctttcgta catgaccttg tccatcttga ggcgtgaaat atcggcgata acgacgttcc
     3601 cgttgtagat ctcatccttt atccttgggc tgtctttgat atcccctatt gcggctatct
     3661 ttacaaacat ggcaggggct gacgcctcgg cctgttcgaa agcctcgagg tcgagatcca
     3721 tgtagtcgtc ctcagtggag ggagttgact tgcccagaag cgaatcgaga aactttacca
     3781 tgggaaaaaa tgtcttttat tttttattta atagtatcta tttcaaaaat gacgatattc
     3841 atggcccggg attactcctg ggggatttta ccgcttctaa aattccacgt tccagaggtc
     3901 gtcaccgata taatggagtg tttttaccat cttgccggat gttttcccca tcagatcgcc
     3961 ggcattataa agggcaacac caagcgcaag gggctttcca tgccgctctt ccaccacctg
     4021 taccggacag cctgccttca catcctcact aaccgataca attccgggcc gcatgatatc
     4081 tgccccgttg acgacaaaag ggaccgcccc tgaatcgaca acaacccgtc gttccgggaa
     4141 cggatgttcg agcaggccct gaagggtggg aaagacccac tcccccgaat ccatcagctg
     4201 gggtttcttg tcgatgaggt agagcgcaaa ccctgcattc gtctcgagta tttcgacccg
     4261 atcgctcatg aagagtgaag cggactcccc tatctgggca caaagcccgg aaaagagggc
     4321 tgatgcctgt gatttcctga tgctgtgcct ttttctcccg gtgatcttcg ccatcgcgtt
     4381 ccactatatt tctcgtcccg atcatgaaaa agcatttcag caagagtggt gtatatgagt
     4441 gcgataagac aggtacactt ccttgaggga gcccgggcgc tcgccaagtt ttaagtatct
     4501 ttcttacgca catatattac tccacagtga tggaacacag ggaattcgta tgacaaaacg
     4561 gccgttggat attttggatc aggtgctgaa ccggcaaccc gtaatcgtct ccctcaaggg
     4621 cggcagggaa cttcgtggcg tattgcaggg atatgacgtc catatgaacc ttgtactgga
     4681 taaagccgaa gaggttgagg gtgggcaggc gcggagcgtc gggaccctca tcgtccgcgg
     4741 cgacaatgtg atctatatct ctccatccat ggactgatag tgcggaaggt gaagcatgtc
     4801 aaagggcact ccatcaatgg gaaagcgttc aggcaaggtc catattgctt gcagaaggtg
     4861 cgggagcatc tcctatcatc tgcggcaccg gacctgttct gcatgcgggt ttggtaaaag
     4921 cacgcggatg aggaagtaca actggataaa caagagacca aagatcccga cccattagag
     4981 agcgaccatg tgtggaatcg ttggcatcag ggatgctggc ggtgtgtcgt tctcactcta
     5041 ttatgcattg tttgctcttc agcacagggg gcaggagagc gcagggatct ccacttttga
     5101 tggggttcag ctctataaac acaaggggca gggtcttgtt gccgaagtct tcgatccagc
     5161 actgttgcgg gggcttgaag gctatgttgg aatagggcat gtacggtatc ccacgaccgg
     5221 agcaaacaag ccggagaacg cacagccgtt caattttctt tttcgtgatc acatcatctc
     5281 cattgcccat aatggcaacc ttgtgaatgc ctgcgaactc agagacgaat acgaggcacg
     5341 cgggcaaatc ttctgcacga ccacggacac cgagctgatc gcaaaggtga tcacggacga
     5401 gatgagcaca tcgggatgca tcgaggatgc cgttcaccgg tgcatgcgga tactcaaagg
     5461 ttcgtattct gttgtcctga tgatggacgg gatactgtac ggattcaggg atccgcttgg
     5521 aatcaagccc atgtgcatcg gaaagaccag ccaggggatc gtgctggcat cggagagtgt
     5581 ggcggtcgat gcccttggtg ggacgttcct tcgtgatctg aaacctgggg agctggtatg
     5641 tatcgatgag gatgggatgc gcgccatgca gatagcagta tcggactgcc gtgcccactg
     5701 tatctttgaa tacatctatt ttgcaagggc cgactcgatt cttgatggtg cccttgttta
     5761 tgatgtaaga cggaatatcg gaggaaagct ccatgaagaa gctcccgtca atgccgattc
     5821 catatgcccg gtcccggatt cagggaccgc atatgctatt ggatattcaa agacatccca
     5881 tatcccgttc gtagaaagtc tcatgaaaaa caggtacatg ggaaggacct ttatcatgcc
     5941 ctcgcagatt gagcgggaga aggccgtcag gatgaaactg aatccgattg gcgatcacct
     6001 gaaagggaag tcaatcgtac tcgtcgatga cagtattgtc cgggggacca cgtcacggag
     6061 gatcatcaat atcatccgcg acgccggtgc aaaagaggtg cacgtaagga ttggatctcc
     6121 ggccatcaag gctccctgct atcttggtgt tgacatcccc acccgtgagg agctgattgc
     6181 cagcgacaag aacgaggaag aagtccgggg acgaatcacg gcaaactccc tccaccatat
     6241 ctccctcgaa gcattggttg aatccatcgg gatagaaaga aaacacctct gcacaggatg
     6301 tctcaccggg tgttacccgg tttccattac aggggaacag tcacaacctc ccgcaataga
     6361 tttccttgac gggacctacc agacaaagct cgagctcttt gaataacgac ctttgcgcaa
     6421 aactttttta aaaagtaagt agcgagggat ggatttgaac catcggtcta cgggttatga
     6481 gcccgtcggg atatcctgac taccccacct cgcttccggc aggagtataa tattgacaat
     6541 ctcgggacat atagtttact cctgtagtcc gggacagatc cctctgggca gggattaaat
     6601 ggtctaccac caaaaggatt tcatggatga tgagctgcag cgtatcaggg aaaagaaact
     6661 ccgtgagatg acggagcatc tccatggggc agaggttccc ctcgtacatc ctctcaatgc
     6721 cgcaaacttc cgccagctgc ttacggccaa tccgtttctt gtcatagatt tctgggctga
     6781 gtggtgcggg ccatgcaggt tggttgctcc ggtgattgaa gaactttccc gcgaatttgc
     6841 cagcatcgtt acctttgcaa agtgcaatac cgatgaaaac cccggtatcg cgggaggatt
     6901 tggaatcacc gccatcccga ctatcatcct gttcagggac gggacggcag ttgatatggt
     6961 ggtgggggct tatcccaaag aggctttgaa ggctcggatc aagaaagcat ttcaccttcc
     7021 ctgacacgtg attctgcagc atcaccacta tccatcctat tatatcacca gattgcagta
     7081 tcataatgag atggtgaatc ccatgcgcct gattgtgggg ctttccgggg ccagcggtat
     7141 catctacggt atccggatgc tcgaggtcct gcacgacctc aaaatcgaga ccgatcttgt
     7201 gatgactggt atggccgaga aaatggtgga gatcgagaca agccggagtg ttgagcaggt
     7261 cagggatttt gcaacacggt gccatgataa cagtgatttc acttcagtgc ttgcgagcgg
     7321 agggtaccgg acgatgggga tggtggtcct tccgtgcagt atgaagaccc tggccggtat
     7381 cgcctgcgga tttgccgaca accttctcct ccgggctgct gactgcaccc tcaaggaacg
     7441 gcggccgctc atcctcgttc cgagggagac acccctgaat ctgatccagc tgaagaacat
     7501 ggttaccctg gctgaatcag gggttaccat cctcccggca gcgccagggt tctaccaccg
     7561 gcctgaaacg atagatgacc tggtcgatca tatcgtcggg aaaacgcttg acgtattcgg
     7621 tgtcgatcat cacctgtatc accgatggcg ccaggatttc tgaacgttct ccctgtttta
     7681 tctaaaaatc ccgatatttt taatcatatg attctctgaa aggggacaag tgtccaccat
     7741 gagagtggcg gaagatcttc acataaaccc ccgtagtcga tactctgtcg agaataatgt
     7801 gtgaagtgcc catgatacca atggatcaca aaccgtccga atgaacatct cggattgtga
     7861 tgttaatgcc aaatggcgga gctacatcca ttggaaaaaa ggggggtatc aggcagtctg
     7921 cgctcccggt gcgcatcctc cccgcagata ctggagagag aggattgtga ttgctgaaag
     7981 ggggagcgcg ataacgatcg gatctatggc actccaggga tatgcgagga ctcctactgt
     8041 tccgaggagg gcttttgaga ggccgaacac ggctgcatac tttgtattga caaagaaagc
     8101 ccagatgaac caggcaagcg ctccgatgac aaggcttgct ttcgctgcca ggggatccgg
     8161 tctcttgcag tacaccgcaa cagcgaatat ggggaggaat gcagatgcac aaagacccat
     8221 gaacattgca gttgctatcg cgatgatact ccccggcatc aggaatgcga tgataacact
     8281 gataacgatc attgcaagga cccctgcctg gtttgcacgg agggagaggg cacactccct
     8341 cccccttccc cagacatcgc agatcagggc ggttcccatt gcatggaatt gggatgacag
     8401 ggttgacatc gccgcggcga ggagggtcac catgaaaagc acgacgaacc actccggcat
     8461 ggcgctgttg ataaatatgg gaatgatcgt gtcgatattt ccggccgtag caacctgggc
     8521 agcaatcttc ccctgggtct ggtagaaata cacgttcgtg agggcgccga ccgtgaatgc
     8581 taccccggtc atcatcagga tgaagggtcc accaaccagc acagccctgt tgagggagcg
     8641 gttgtccttt gccgtcatga acctgaccac cagctggggt tgtgcaagga caccgatccc
     8701 cactccaagc accaaggtgg tgatgaccgt ataccagatg ggcgatccga gatcgggcat
     8761 tacggtccag ccgttcatcc cctgctctgc cagcttgacc gggaccagat ctgccatgtt
     8821 tgtcagtgca gtattggcca cggtaactcc tccgaggagg agataggtcg caatgaggag
     8881 gaatgtcatg ccgacaagca tgatggcacc ctggaccgca tcagtgtaca tgaccgcgat
     8941 caggccaccg aatacgacat aaatggccac gatgagtgca aaacctatca aagaaatgcc
     9001 ataggatatc ccgagcgtct ccttgatgaa ctgggctccg ccgatgagca ctgctgcggt
     9061 ataaaggggc atgccaatga gaatgatcac cccggtcaga acctgcatga actgggactg
     9121 gtagatcttc cccatcaggt ccggaaatgt gatagccttg agccgctgcc ctatctcccg
     9181 cgtctttttt ccgaagacga caaaagcgat caagatcccc acccctatat tgaagaccgt
     9241 gagccagatc aaccccatcc caaggtttgc tgcctggcca ccgaagccga cgatagcaga
     9301 ggtgctgata aacgtcgccc cgtacgagag cgcaatcacg acaggatggg cattcctgcc
     9361 ggcgacaagg taatcctcag tatttctggt tttcttgtat ccgatgtagc ctaggatgag
     9421 catgatcacc acatagattg cagtgacaac aacgaccatg gtagtgctga cagccattcg
     9481 tcaatcgcct cctttgttcc aatacgcgta accgtatatc atacaagcaa gggcgagtgc
     9541 taggcagagc agatacgcga tccagatctg cgggtcaggg attccaaaca tgttacaaac
     9601 cttctggtaa tctcatgaag tgtccattac aggggaaccg gctcgaaaag ctggtcccct
     9661 acctaaagaa gaaaataaaa aatacggatg attgctgcag gaccgattgt gagttgatat
     9721 cctgtaagcg ggacattccg gtttacctga tctttcaggt cactcttttt tctatttaag
     9781 ccttttttaa ttgttccgaa gcaaatcacg gaaatctccc gggattctct ctcgcaggaa
     9841 taaacccctg cctggaggct tgcacaggga gtgtccggaa aatactcact ttcagtgaat
     9901 ggttctcttt tcttgggccg gacaaccgta tttttccggc cgaaaaccag atgaaccgtc
     9961 cggctccata tggaggtagc gtatatggat accttttccc gtt
//
LOCUS       DAOOCO010000054         9827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6522_length_9827_cov_4.212239, whole genome shotgun sequence.
ACCESSION   DAOOCO010000054 DAOOCO010000000
VERSION     DAOOCO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_9827_cov_4.212239"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..252)
                     /locus_tag="PK154_04480"
     CDS             complement(25..252)
                     /locus_tag="PK154_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10352.1"
                     /translation="MKNTDHVPDMIDRVKVPRKVYQELMMINREIHYPDDYPLAVKKA
                     DNNGFIHAAEWLKKNEELYKRGFVRGFEPAD"
     gene            389..2077
                     /locus_tag="PK154_04485"
     CDS             389..2077
                     /locus_tag="PK154_04485"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10353.1"
                     /translation="MNIRSISSAGFTSAILLFCIVVGASFLCGSIENTGDPAAGSSGI
                     LQVGHNVQPAYENQEPVSSADVPVANFSAKAGAITRFSVFFDGTLSKGSDLSYQWSFE
                     DGSVLSSPKPVRFFKGPLVGLVTLKVSNAEGSDSITQKVTIDRNGEVSVERYDKPDIP
                     KEIPPVITPATIPDMNETIPGVSESPDYLQKFIEGAQFMEEGDLSGAIEALSISITLN
                     QSFPDAYVARGMAEFMTGYYHFYEFRGKEQLLMAVDDYTRALEQDPGNIDALLGRGAA
                     WIYLGEYHSWRSYIGNDTVFHYYENAIKDFNKVLETDPDNIDAINGKAYATLVIGSGN
                     PAKKANTLMVESAKKDIERSIRLNDDNPRAHFILGLYYDHEGLYPSAITEFSKAIEQD
                     PTEAWYYEWRGFEKFQKGDYSDAIKDYSTAISLQPRFAQAYNIRGMTRAFLMNVDDRS
                     PELSDFNQAIEINPEISEFYRNYALGLANWKYWDKSLNEHAVDVLATASVLDPTDYRI
                     HGDRAYILTGLNRNHEATQELIEYQRRSLTNEEKITASDLLAYNNVARYYVNTW"
     gene            complement(2311..2385)
                     /locus_tag="PK154_04490"
     tRNA            complement(2311..2385)
                     /locus_tag="PK154_04490"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2347..2349),aa:Glu,seq:ctc)
     gene            2611..3723
                     /locus_tag="PK154_04495"
     CDS             2611..3723
                     /locus_tag="PK154_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW10354.1"
                     /translation="MAGIEDEIRALEEEIQRTPYNKATAKHIGRVKAKIARLKDEAVQ
                     RAMKSAGGGDGYSVKKSGDATVVLVGFPSTGKSTLLNTLTGTKSEVAAYAFTTLTVVP
                     GAMEHRGAKIQILDIPGLIAGAAMGKGRGKEVIGVVRSADMIVILVDVFNSSHVDVLM
                     RELYDAGIRINKQKPDITIKKTSMGGIRVNAVGALDLDVDEIRSILAENKMMNADILI
                     RGNATQDEVIDAMLANRVYIPAFIAVNKVDLIDEKNTEAIATDLNERFLMKPEMISAH
                     TGFHMAEMKDRIYDSLGFMRVYLKPQSEAADLEEPLIIRQGSTVEDVCRKLHRDFVDR
                     FRYARVWGHSVKHPGQRVGLTHRLKEGDLLTIIIEH"
     gene            complement(3809..4936)
                     /locus_tag="PK154_04500"
     CDS             complement(3809..4936)
                     /locus_tag="PK154_04500"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HPW10355.1"
                     /translation="MDLIEVEEGTTKFFIPLQDDSSHFPPGSAPIFYNPRMEMNRDAT
                     VLFVAVVKPLEYLDAMGASGVRGLRVATECGVAVTINDRDHKAYELIQRNALDHSLPV
                     TVTCRNVHSLLAERRYDSVDIDPFGSPAPFIDSAVRGCGRFLMVTATDTAPLCGAHLN
                     AGIRRYGAIPANNEYHSETGLRTLLSFIARETVKFDRGIEPLFCYAKEHYVRAHFRLL
                     NSATAADKTVARIGYILQCTRCPGRIEQAGLIPKTVKCEECGAGMKPIGPLWLGEIQD
                     RGVISAMKNVRGSMKLNTVHRLSLLLDLISDELPTSSHYDYHALSKVARISPRPLSEV
                     IAILREKGYQASRAHYSGTALKTDAAAEVIIRVLKEKTKLC"
     gene            5001..5075
                     /locus_tag="PK154_04505"
     tRNA            5001..5075
                     /locus_tag="PK154_04505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5036..5038,aa:Arg,seq:cct)
     gene            5509..6348
                     /locus_tag="PK154_04510"
     CDS             5509..6348
                     /locus_tag="PK154_04510"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPW10356.1"
                     /translation="MKIPGEICLIRPINALVAGLAAILSYFIASGTLIPGTIILLAIV
                     FLITAGGNTINDFFDVEIDQINRPDRPIPSGEVSKYHAGILALVLFACGILLSAFTNS
                     LCFIIAVFNALLLVLYAARLKRKIFIGNMAVSYLTASIFLFGGAFYGIDGFEKISAIA
                     LMTFFAILSRELLKTAEDVEGDRAAGALTIPVVYGIRPTVFLALAFALCAVAVSFYPF
                     RWWGSLYLGGILIVDAIILFAATRTLGCLHPACIREKRASQLLKIGMFSSLIVFTLSS
                     ALL"
     gene            complement(6359..7072)
                     /locus_tag="PK154_04515"
     CDS             complement(6359..7072)
                     /locus_tag="PK154_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HPW10357.1"
                     /translation="MVIENSILSQFIFLIGEITLLFILGSIVFAILMVTLALISIKRG
                     KIYFPSLIKSGVVLVEGLMKALFRLFGLEDQQVNSFFIELHNSMNKRAFEAVPVSDRA
                     IFLPQCLRSSKCPAHLTPEGLKCKCCGLCMIGYWLPLLEKMGYRVFSVPGSSFIKRMV
                     RKYRPKAIIGVGCMGEVKEGLEMSDKLGLISMGVVTLKEGCVETYLDWEMLLEIAKLG
                     VDPGTIPPDLITLPKNNNI"
     gene            complement(7097..8143)
                     /gene="mtnA"
                     /locus_tag="PK154_04520"
     CDS             complement(7097..8143)
                     /gene="mtnA"
                     /locus_tag="PK154_04520"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPW10358.1"
                     /translation="MNSFVTISWDEETKSIVLIDQTSLPTSCRMIRCRTVDRLITAIR
                     RLEVRGAPALGIAGAYGVALAAFSIKLTSFPGYIRRVKNAGFAIGNARPTAVNLSWGV
                     HRVLTKAEELATSIEEYRQIALVEAQTVAEEDSAACHALGDYGAALLPDTGTILTHCN
                     AGALACREWGTALGIVRSAVASGKNISVIACETRPLLQGARLTAWELKRDGIPVTVIP
                     DSAAAHLMRKDKIDCIIVGADRITRDTVFNKVGTYMHALCAKSHDLPFYVAVPSSTFD
                     SIHAGSEIEIEERERDEVARFSGRITVPDGVSVINPAFDATPLSLVSALVTEKGIISP
                     ASDWMQTGKIHRII"
     gene            complement(8145..9419)
                     /locus_tag="PK154_04525"
     CDS             complement(8145..9419)
                     /locus_tag="PK154_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPW10359.1"
                     /translation="MGSVVIIGGGVTGIQAALDLANHGVRVHLIEREPSLGGHMAQLD
                     KTFPTNDCSMCILSPKMLDVSRHPDVVIHTCSEVKKIEGQKGHFFVTVQKNPRYVNED
                     LCNGCGDCISICPVEVYNRFDAGIGVRKAIYKPHSQAVPDIVIKDPEHCIECGLCYDI
                     CGREAIRKDDLGSESVIEAASIIIASGYDVFDAALKEQFGYLRHPDVVTSLEFERMMN
                     ASGPTGGSIRRLSDGTTPRHVVFIQCVGSRDIRAGRPYCSGVCCMYAMKNAMLIREKY
                     PDTEVTILYMDIRAYGKGYEEYFERAQQMGVRFLRGMPGEIIGRGGEMIIRVENTETG
                     NMEELRPDLVVLSVGISPASDSETIAARCGIPIDKTGFFTSLDEKVDCVATIKPGIYI
                     AGASVSPKDIPDSVAQGQAAAMRAFLDIVKDC"
     gene            complement(9419..>9827)
                     /locus_tag="PK154_04530"
     CDS             complement(9419..>9827)
                     /locus_tag="PK154_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPW10360.1"
                     /translation="DETGIWHGAVIRADKDTIVIGNRSNVQDNAVLHTSVGYPVVIGN
                     EVSVGHGAILHGCRIADRVLVGMGAIVLNGAEIGQDSLLGAGSVITEGTKIPPGSVVV
                     GVPGKVIKQVSDEQRAHIVRNARNYWDLAGRYL"
BASE COUNT         2520 a         2466 c         2372 g         2469 t
ORIGIN      
        1 ttcaagacac cgatctgcat aaagtcagtc tgcaggttca aatccccgga caaatcccct
       61 tttatagagt tcttcattct tcttcagcca ttctgcggca tggataaaac cgttgttatc
      121 agcctttttg actgcaaggg gatagtcatc ggggtaatga atttcccgat taatcatcat
      181 cagctcctga tacaccttgc gtgggacttt cactctgtcg atcatgtctg gtacatgatc
      241 ggtattcttc attgagataa atatagatta ttcaccggtt catggttggt aaatgaattt
      301 atgcaatgaa tacgccggaa agaaatgaat atacacttca gtatgaaaga tgataccatt
      361 ataattccag tcaggaggta gaatttttat gaatatccgg tcaatctctt ctgcagggtt
      421 tacctctgct atcctgttat tctgtatcgt tgttggagca agcttcctct gcggcagcat
      481 cgagaacacg ggtgatcctg ctgccggatc ttccgggata ctgcaggtcg gacataatgt
      541 ccaacctgcg tacgaaaatc aggaaccggt ttcttctgcc gatgtgcccg tggccaattt
      601 ttctgcaaag gcaggcgcaa taaccaggtt cagcgtgttt tttgacggga ccctctccaa
      661 aggatcggat ctgagttacc agtggagttt tgaagatgga tctgttcttt cttcaccaaa
      721 accggtaaga ttttttaaag gcccgttggt tggattggtt accctgaaag tgagcaatgc
      781 cgaaggaagc gacagcatca cgcagaaggt tacgatcgat aggaatggag aggtgagtgt
      841 agagcgttat gataagccgg atattcctaa ggaaatacca ccagtcataa ctcccgccac
      901 gatccccgac atgaatgaaa caattcccgg ggtcagcgaa tctcctgatt accttcaaaa
      961 attcatcgaa ggtgcccagt tcatggaaga gggagacctc tctggtgcaa ttgaagccct
     1021 gagtatatcg attacactca accaaagctt tcccgatgct tatgttgccc ggggaatggc
     1081 tgaattcatg acgggatatt atcacttcta cgaattccgt ggaaaggaac agttgctcat
     1141 ggccgttgac gattataccc gtgcactcga gcaggatcct ggaaatattg atgcacttct
     1201 cggaagggga gcagcatgga tctaccttgg ggagtatcat tcatggcggt cgtatatcgg
     1261 aaatgatacc gtattccact actatgagaa cgcaatcaag gacttcaaca aagtccttga
     1321 aaccgatccg gataatatcg atgccatcaa cggaaaagcg tatgccaccc tagttattgg
     1381 ttcagggaat ccggcaaaga aagcaaacac gctcatggtt gaatccgcaa aaaaggatat
     1441 tgagcgttca atccggctga acgatgataa tccgcgtgct cattttatcc ttggattata
     1501 ttatgatcat gaaggcctct atccttcagc catcactgaa ttttcaaagg cgattgaaca
     1561 ggatcccacg gaagcatggt attatgaatg gaggggattt gaaaagttcc agaagggaga
     1621 ttattccgat gctatcaagg attattcgac ggccatctcc ctgcagcccc gctttgcaca
     1681 ggcatataat atcaggggaa tgacgagggc atttcttatg aatgtggatg atcgttctcc
     1741 tgaactgagt gattttaacc aggcaatcga gattaatccg gaaatatccg aattctatcg
     1801 gaattatgct ctaggacttg caaactggaa atactgggac aaatcgctga atgagcatgc
     1861 ggttgatgtg cttgcgacgg catctgtact tgatccaacc gattaccgga tccacgggga
     1921 cagggcgtat attcttacgg ggttgaacag gaatcacgaa gccacccagg aacttataga
     1981 atatcagagg cgttcgctca ccaatgaaga gaagataacg gccagcgacc ttctggcata
     2041 taacaacgtt gcacggtact acgtgaacac gtggtaaaaa gcacaaggcg ccacgccttt
     2101 ttttatatga aaatgcatcc ggcattttca tcttttatgc gtgtggccct caaaggaatc
     2161 atgttatttt atcaccgtaa atcgttaccg gatactcggt tatccggttc tgattactgg
     2221 gaaagtgtct gcgcaggcca attcactaga cctcatgagc taatactgct ggataccaga
     2281 aagacaggaa acagaaccat cagagaatag tgctgctatg gggattcgaa ccccagtcgc
     2341 gggcgtgaga ggcccgcatg attggccgga ctacactata gcagcaactg ctctattcta
     2401 tgaggcatat tcgtacttaa atctgttgat atctgccctt ccggaaccaa atacgggctc
     2461 tggatgggat ttcccccccg ttccactatt ttttctacac caaggaacag gagtataaaa
     2521 ttcagcagaa actggaattc tattattcaa ctctccgcaa agacccacaa ctatattcct
     2581 ttccttccgc ctatatatag gaacagcgaa atggcaggta tcgaggacga gatccgggcc
     2641 cttgaagagg agatccagcg gacgccctac aacaaggcaa cagccaagca tatcggcagg
     2701 gtgaaggcaa agattgctcg gctgaaggat gaagcagtcc agcgggccat gaaatctgct
     2761 ggaggcggcg atgggtattc cgtgaaaaag tctggggatg caaccgtcgt tcttgtcgga
     2821 tttccctcaa ccgggaaaag tacactcctc aatacgctta ccggcacaaa gagcgaagta
     2881 gccgcctacg cttttacgac gctcacggtt gtccctggtg ctatggaaca tcgtggtgca
     2941 aagatccaga tacttgatat acccggtctt atcgccggag ctgcgatggg aaaaggccgc
     3001 gggaaagagg tcatcggtgt tgtaagaagc gccgacatga tcgtaatcct cgttgatgtc
     3061 ttcaactcat cccatgtcga tgtcctgatg cgggagctct atgatgcagg tatccggatc
     3121 aacaagcaaa aacctgacat cacgatcaag aaaacctcca tgggcggcat cagggtcaat
     3181 gccgtaggtg ccctcgatct tgacgtggat gagatacgct caatccttgc agagaataag
     3241 atgatgaatg ctgatatcct gatccgtggg aatgcaaccc aggatgaagt gatagatgca
     3301 atgcttgcaa acagggttta tattcctgca tttatcgcag tgaacaaagt ggacctcatc
     3361 gatgaaaaga acacggaagc aattgccaca gatttaaatg agcgtttcct gatgaaacct
     3421 gaaatgatat cagcccatac cgggtttcac atggcagaaa tgaaagaccg tatttacgat
     3481 tctctcggtt tcatgagggt atacctgaaa ccgcagagtg aagcagcaga tctcgaggag
     3541 cccttgatca tacggcaggg aagtacagtg gaagatgtct gccggaaact gcaccgcgat
     3601 ttcgtagatc ggtttcggta tgcaagggta tggggacact ccgtgaaaca cccggggcag
     3661 agggttggcc tcacccatcg cctcaaagag ggggacctcc tgaccataat cattgaacac
     3721 tagacggtat cggatttcct gatgaggacc tggttcaaga aaatcggtcc tcctgttcct
     3781 tattaccctg atacgtcgag acttcccctt aacacaactt cgtcttctct ttcaagaccc
     3841 tgatgatcac ttcagcagcg gcatcagttt ttaacgcggt tcctgaataa tgggctctgc
     3901 ttgcttgata gcccttctcc cggaggattg caatcacttc agagagaggt cgaggcgata
     3961 tcctggcaac ctttgagaga gcgtgataat cgtaatggga tgaagtaggg agctcatcac
     4021 ttatcaggtc aagaaggagg gaaagccggt ggacggtatt cagtttcatg gagccccgga
     4081 cattcttcat ggcagatatc acgcccctgt cctggatctc tcccagccac aaaggtccaa
     4141 tcggcttcat acctgccccg cattcttcac atttcactgt ttttgggata agcccggcct
     4201 gttcaatgcg tcccggacac ctggtgcact gaaggatata tccaatcctt gcaacggtct
     4261 tgtcagccgc cgttgcgcta tttaaaagac ggaaatgggc cctgacataa tgctcctttg
     4321 catagcagaa cagaggttct atcccccggt caaatttcac tgtttccctt gcaatgaacg
     4381 acagcaacgt ccgcagacca gtctcggaat gatattcatt gtttgcaggt attgcaccat
     4441 atcggcggat accggcattg agatgagccc cgcaaagagg cgcggtgtca gtagcggtta
     4501 ccatgaggaa cctcccacaa ccccgaaccg ctgaatcaat gaacggagca ggtgatccaa
     4561 agggatcaat atcaaccgaa tcatatctcc gctctgcgag aagtgaatgt acatttctac
     4621 aggttacagt gacaggcagg gaatgatcca gagcatttct ttggatcagt tcgtatgctt
     4681 tgtgatcccg gtcattgatc gttaccgcga caccacattc tgtggcaacc ctgagccctc
     4741 tcaccccgga tgcacccata gcatccaggt actccagggg ttttacaacc gcaacaaaga
     4801 gaaccgttgc gtccctgttc atctccatcc gcggattata aaagatcgga gcactgccgg
     4861 gagggaagtg ggaagaatca tcctgaaggg gaataaagaa cttcgtggtg ccttcttcca
     4921 cctcgataag gtccataatt ctttactctg gtagaattgc aaaaacctta taccttctcc
     4981 tcatctattg ataatgctgc gggcgtgtgg cctagtctgg acagggcggc agcctcctaa
     5041 gctgtaggtc gggggttcaa atccccccac gcccgttttt attaccaaat tgcgttgctc
     5101 ccgatatgga tcaaaaagaa agggtatagc actattttta ccacctatct ttcccataaa
     5161 tcacgtcgga aagtatacat ttgttggagt tctcaaatct atgcaaaaaa agtgatacac
     5221 cattcgatat gaacaatgaa gagatgtatg tgctcgataa cgccctatcg catcgtgggg
     5281 gacgccacag cggctggggt tagccgaagg cgcaaccccc gggggtgttt aaaaagacat
     5341 gaaacacatt gatgggaaaa aatggtggat gaaatgccac tatcccctaa agaaattatg
     5401 gtccgatgca aaaagtcagg gaggagccag ctcaatttca ggaaacgata tcctttatct
     5461 tgatatggcg ggaagtcgat cagataatca ccggaggttt ggcggtaatt gaagattccc
     5521 ggtgagatct gtctgatccg acccatcaat gcccttgtgg cagggcttgc tgcaattctt
     5581 tcatatttca ttgcctcggg gacactcatt cccggaacga tcatccttct ggcgatagta
     5641 ttccttatca cagcaggagg aaataccatc aatgatttct ttgacgtgga aattgatcag
     5701 atcaatcgcc cggatcgccc aatcccttcc ggagaagtga gtaaatatca tgctgggatt
     5761 cttgctcttg tcctctttgc atgtgggatt ttgctgtcag ccttcaccaa ttccctttgt
     5821 ttcatcattg cagtgttcaa tgcactcctc ctggttctct acgcagccag gttgaaaagg
     5881 aagatattca tcggtaacat ggcggtctct tatctcactg catctatctt tcttttcggt
     5941 ggcgcttttt atgggattga tggcttcgag aagatttctg cgattgcact catgactttt
     6001 tttgctatcc tttcccggga gctcctgaag acagccgagg atgtagaagg ggatcgtgcg
     6061 gctggtgccc tcacgattcc tgttgtatac ggcatacgac ccacggtctt cctcgcgttg
     6121 gcttttgcac tttgtgccgt ggcggtaagc ttctatccgt tccggtggtg ggggagtctc
     6181 tatctggggg ggattcttat cgttgatgct attatccttt ttgccgcaac gaggaccctt
     6241 ggatgcctcc acccggcctg tatcagggag aaaagggcaa gccagttatt gaaaattggg
     6301 atgttctctt ctcttattgt ctttaccctc tcctctgcat tgttataaag cccgatcact
     6361 atatgttgtt attcttcgga agagtgataa ggtcaggagg aatggtaccg ggatcgacac
     6421 ccagttttgc gatctccaag agcatctccc agtcaaggta cgtctcgacg catccctctt
     6481 tcagggttac gactcccatc gagatcaggc caagtttgtc cgacatttcc agcccttcct
     6541 tgacctcccc catacatcca acccctataa tggcttttgg cctgtatttc cggaccattc
     6601 ttttaatgaa cgatgatcca ggaacggaaa aaaccctgta tcccatcttc tcaaggagtg
     6661 ggagccagta cccgatcatg cacaatccac aacacttgca cttgagacct tcaggtgtca
     6721 ggtgggccgg gcattttgaa gaacggagac attgaggaag gaatattgcc cgatcactga
     6781 ccgggactgc ctcaaatgct ctcttgttca ttgagttatg aagctcgatg aaaaaagaat
     6841 tcacctgctg gtcttccagt ccgaataacc ggaagagtgc tttcatcagc ccttcaacaa
     6901 gaaccacccc ggacttgata agagagggga aatatatttt tcctctcttt atcgagatca
     6961 gggcaagggt gaccatcagt attgcaaaaa cgatgctccc aagaatgaac aggagggtga
     7021 tttcaccgat caggaatatg aattgactga ggatcgagtt ctcaatgacc atctggattc
     7081 aaggtatccc gggattttat ataatcctgt gtatctttcc agtctgcatc caatctgacg
     7141 ccggactaat aatccctttt tcagtgacaa gagcagatac gagagagagc ggtgtcgcat
     7201 cgaacgcagg atttatgacg gaaacgccat ccggaacggt gatcctgcca gaaaagcgag
     7261 ccacttcatc gcgttcacgc tcttcgatct ctatctcgct cccggcatga atgctgtcaa
     7321 aggttgatga ggggactgct acataaaacg gcaggtcatg ggactttgca cagagagcat
     7381 gcatgtacgt accgaccttg ttgaagacgg tatcacgggt gatccgatcg gctccgacaa
     7441 tgatacagtc aatcttgtct ttcctcatca gatgggctgc tgcagagtcc gggataacgg
     7501 tgacagggat cccgtcccgc ttcaactccc atgcagtgag acgggctccc tggaggagag
     7561 gccgggtctc acaggcgata acactgatat tctttcctga agccactgcc gaccgaacaa
     7621 tacccaatgc ggttccccat tcccggcatg ccaaagcacc agcattgcaa tgtgtcagta
     7681 tagtcccggt atcagggagg agggctgccc cataatcccc gagagcatgg catgctgccg
     7741 agtcctcttc agcaacggtc tgtgcctcca ctaatgcgat ctggcgatac tcctcgatcg
     7801 aggtagccag ctcttctgct tttgtaagaa ccctgtgtac accccacgac aggttcaccg
     7861 cagtaggacg agcattcccg atagcaaagc ccgcgttttt taccctgcga atgtatccgg
     7921 gaaaactggt cagcttgatc gaaaaggccg caagtgcgac accatacgcc cctgcaatac
     7981 ccagggcggg tgctcctctc acctcgagcc tccggatcgc ggtaatcaga cgatcaactg
     8041 tccggcatcg tatcatccgg caggaggtgg gcaggctggt ctggtcaata aggacaatgg
     8101 attttgtctc ttcatcccaa gagatcgtca caaatgagtt catttcagca gtcttttaca
     8161 atatcgagaa atgcacgcat cgcggccgcc tggccctggg ctacactgtc agggatatcc
     8221 ttcggggaga cagatgcccc ggcaatataa atgccgggtt tgatggtggc gacacaatca
     8281 accttctcat ccaatgaggt gaaaaatcca gttttatcaa ttgggattcc gcaacgggca
     8341 gcaatggttt cagaatcact agcaggactg atcccgactg aaaggacgac cagatcaggg
     8401 cggagttcct ccatatttcc ggtttccgta ttctccaccc tgatgatcat ctcacccccc
     8461 ctcccaatga tctctcccgg catgcccctg agaaaccgca ctcccatctg ttgtgccctc
     8521 tcgaaatatt cctcgtatcc cttgccatat gcccgtatgt ccatgtagag gatggtcact
     8581 tcagtatcgg ggtatttttc acggatgagc atggcattct tcatggcata catgcaacag
     8641 acccctgaac agtacggacg tccagcccgg atgtcccgtg atccgacaca ctggatgaat
     8701 acaacatgtc gcggagtggt cccatctgac aagcgccgaa tcgatccgcc ggtggggccg
     8761 ctggcattca tcatccgctc aaattcaaga cttgtgacaa catcagggtg acgaagatag
     8821 ccaaactgtt ctttaagagc ggcgtcgaag acgtcatatc cggaggctat gatgatgctt
     8881 gcagcctcga tcaccgattc ggaaccgagg tcatctttcc ggattgcctc tcgtccacag
     8941 atatcataac agaggccgca ttcgatgcaa tgctcagggt ccttgattac aatgtcgggg
     9001 acggcctggg agtggggttt ataaatggcc tttcttaccc cgatacctgc atcaaagcgg
     9061 ttgtacactt ccacgggaca gatactgata cagtccccac agccgttgca gagatcttca
     9121 ttaacatatc gtggattctt ttgtaccgtg acgaagaaat gacctttttg cccttcaatc
     9181 tttttcacct cgctgcaggt atggataact acatccgggt ggcgggaaac atcgagcatc
     9241 tttggagaga ggatgcacat agaacagtca ttggttggaa aggtcttgtc aagctgggcc
     9301 atatgcccgc ccagggatgg ttcacgctcg atgagatgta ctcggacccc atggttggcg
     9361 agatcgagag cagcctgaat cccggttact ccacctccta taatgacaac gctccccatc
     9421 agaggtatct ccctgcaaga tcccagtaat tacgggcatt cctgacgata tgtgctctct
     9481 gctcatctga gacctgcttg attactttgc cggggacacc gacaacaaca gaccccgggg
     9541 ggatttttgt tccctcggta atcacggacc cggcaccaag cagtgaatcc tgtccaatct
     9601 cagctccgtt cagcacaatt gcgcccattc ccaccagaac ccggtcagct atcctgcatc
     9661 catggaggat agccccgtgt ccgaccgaga cctcgtttcc gatcaccacc ggatacccga
     9721 cactggtatg aagaacggcg ttatcctgga cattggatct attaccaatt acaatagtat
     9781 ccttgtcagc cctgatcacg gcaccatgcc agattcctgt ctcgtca
//
LOCUS       DAOOCO010000055         9773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6562_length_9773_cov_4.541161, whole genome shotgun sequence.
ACCESSION   DAOOCO010000055 DAOOCO010000000
VERSION     DAOOCO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_9773_cov_4.541161"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK154_04535"
     CDS             <1..533
                     /locus_tag="PK154_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW10361.1"
                     /translation="AGGQSYPLDVYIVYEDESGSPMTSKMTTIGIPVHGKISFVVTGN
                     PRPIIAGDKQVIEVIYQNTGSGTAYNAQSRISAVDPFTSNDDTAFLGDLAPGESAVAR
                     YEMSVDNSATPKEYGLDSEIRYKDALGNSQISDTMKVEVTVEETSPVMTILLISVVIL
                     AVIAAGYYFLIVKKKN"
     gene            533..1231
                     /locus_tag="PK154_04540"
     CDS             533..1231
                     /locus_tag="PK154_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HPW10362.1"
                     /translation="MLGWSGPLPEPEIRDIEDMRQVLATPDCQETGPLYFMYRDLALN
                     DCDRQWLHSHHLRYDITAIVSRDICGERVKTKGHYHPLSPSGVGYPEVYEVLRGHAHY
                     LLQERNLLNVILVDALEKDLIIVPPGYGHVTINAGNSDLIMANIVSTDFESQYMEYEK
                     MGGAVYYELTDGRFIKNPAYPSVPEIQVVEAGHLPKVHFSKDGLYETIGTDTLDFLNN
                     PDLGWDLFNEVLRG"
     gene            complement(1209..1955)
                     /locus_tag="PK154_04545"
     CDS             complement(1209..1955)
                     /locus_tag="PK154_04545"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPW10363.1"
                     /translation="MDTDHGCFLGKIEQMIRYVIWRSGATGIVIGISGGIDSAVSAVL
                     CCRALGGERVYGYTLTTSITRDEDIHDAADLCRSLGMHHQVMSIEPVLSSYRQMPGYV
                     EDPYLLGNIMARTRMVILYYHANREHRLVCGTSNRTEYLLGYCTKFGDNAADFQPILH
                     LYKTEIFKYAHELGIPEPIINKTPSAGLWAGQSDEADIGLTYSEIDMALKALETNSWV
                     STNLVEEKVLSLVKKSSHKRANPPNLLALH"
     gene            complement(1965..3674)
                     /locus_tag="PK154_04550"
     CDS             complement(1965..3674)
                     /locus_tag="PK154_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPW10364.1"
                     /translation="MSDSRRGMVWKHFHASRRAKISLFFFLFTIQIIAFTSIFHFAYP
                     VLEGKPISWPNSLLFILETVTTVGYGDLLPFSNELTILFAIIVMITGILLIFMIIPLL
                     LVPYLSTIFLSAPPRKLPHALYNHVVIVGYGELTKALLESIRISDLPVLIVVNTPDTA
                     RLAGRESGAKAYVIWGDYNDPELWSNAWVKNAHSIIACEDERTNAAIILGIREMASGT
                     IIAVIDKLVLGRYLKYAGADYVLSAKHVTGQIIARHAALTTHIDTMVEETINGTMPPR
                     YSPQEENRLRIINLPIIPGSQAYGKTLQELDLFDRYGFFTLLLLRKGNFRLYPTGEER
                     LDSTTMLFLIGHLNQVEEMVRQEFMPHVRERERALIGGFGDVGLSAYHELTSLGIECI
                     AIDRKKHGITQVIGHTEDETTLLEAHIRDAKYFIVALNDDALNIFTTLIAKNLNPEIR
                     ILARANEPESVHKLYRAGADYVALLPSIGGQIIGGVVLSAIVHVILNLPDGKIVVRKS
                     VGDGTGNNIGGVERKTGVRIIGIEGEARSVVKPQPHEMLMRGDAIIAVGNPTDIKELM
                     RII"
     gene            complement(3667..4398)
                     /locus_tag="PK154_04555"
     CDS             complement(3667..4398)
                     /locus_tag="PK154_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW10365.1"
                     /translation="MSGGEESSALLYFLKKILGTRRDVSLFAISIDEGIKGYRDRRIT
                     ANIAESIGVELITDSFDEVLGKTIDEVVREKGDRFSCIFCKGLQGLVLNQIANDHDIT
                     KLALGLNLDDEAKNLLVHIFRGDAERLLKPADSLFRVVPMIRPFLYIPAREVALYAYF
                     NMGGFEIRRCPYSHNTLETDVHSLLNKYNWRHPSTKNALVNLGEHLVSYGVMPLPDAG
                     ICPDCGEPCHGEYQEWGMLRELHHV"
     gene            4612..5469
                     /locus_tag="PK154_04560"
     CDS             4612..5469
                     /locus_tag="PK154_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HPW10366.1"
                     /translation="MAISADRIRELHKYEIRILLILERLMRRYRWVPFDIIKKTLGLS
                     ESEVNYRLGRLMGWGFVRFDTVPYEGYSLIFQGYDALALISLTKKSIIQALGCMIGEG
                     KEAVIYEGLGLSPLALKFHHVGQRSFQSARVTRDYIPEEGNCPWLLASRLSAEREYEA
                     LRTLHPDVSVPLPVDINRHTVVMEFIPGATLNRCSLEDPRDVLLEIIENVGKAYELGV
                     IHSDLSEYNVMHDGFRCYLIDWPQWVSTDHTNAEELLTRDLENITRYFSRRYRVEIPF
                     IEAYQMVTG"
     gene            5466..7418
                     /locus_tag="PK154_04565"
     CDS             5466..7418
                     /locus_tag="PK154_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HPW10367.1"
                     /translation="MRVFGIDIIKGSVRSKSRRPMYALIRMEDGDILGEGEVTGFRLF
                     RILSEEKPDILAVDSLQEIAIDQHDLYTFLQALPPSVRLVQVTGGERKESLGKVASRF
                     NISFNRFDPFDEARTIARVAGLGAGAEVVAFENTSEIIVSRHRSPGKGGWSQNRYVRK
                     IHGSVQQKAREIEMALVAAGLRYRKKETRAFGGNSRVAFEVAAARDQVPVSTYRGADV
                     QVRISGKRLDRITFRPLSGKPRYLIVGIDPGTTTAIATLDLDGNLLALSSSRQMAMSD
                     VIEAIYRVGKPLVIASDVHEMPFSVEKVRRAFNGIAYSPRQDMSVETKHELTAPFPSQ
                     NDHERDALAAAVEAFRSYRNKFQNLIRRVPPGNDLDEVKAGIVRGQSLEQVLSEMKGR
                     PIRPVVEAPKMEIDPRTDERIRILDGTVKRLKGVVKDLQQDLQKKDHEIIRLQGRIKK
                     IRSQQDQVFRRDAEIIKRDAIITGLKKRIRREERNSEKLRKRMKKIRDHDEAGPGEQE
                     VMLKLLPSLTREGIRALSDELGIKEGDLIYVPRIDSWGKNAARELAGSGIGGLLARLP
                     RSSRFDPQLEAIFRDVSIPLLSAEMTGVQVKGGMVTVPRDRLDAAIRAWDDQQRAYEW
                     EKKKTLLEDIYREYRTERGKEMKKVG"
     gene            7411..8274
                     /gene="thiL"
                     /locus_tag="PK154_04570"
     CDS             7411..8274
                     /gene="thiL"
                     /locus_tag="PK154_04570"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPW10368.1"
                     /translation="MDERALKEVVREIIGTEQTLDDCAVFSFGGMLLVATTDMLHETT
                     DFPGGMTDWQVGWMSVAVTLSDIAAMGAQPAIVLLAIGLDNGDRLREILCGAKACCDS
                     FGAELSGGDLDAHQELTVVSSGIGIVEEGAVIRRRGAVPGDLICITGPLGQAHAALEG
                     YSQYTQNLLEPIPRVPEGKRLAAARVSSMMDISDGLVLSLYDLLDANPYGYSIDSFLL
                     PIPEGIPPEKSREYAIFGGGDFELLFTCSPDYFPVPGVDSIIVGEVIPEHEVLVDGIV
                     APCRGYLHRWK"
     gene            8366..8740
                     /locus_tag="PK154_04575"
     CDS             8366..8740
                     /locus_tag="PK154_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10369.1"
                     /translation="MKKRIFAILAAIVLGMLLAAPVSAYYSISVTAGAPDLGNIMSPT
                     VKSLLSQYQSPRSLFGSSSSDLAANAMKNAAQPSKGSVSAYSSGISENQYSKINFNNR
                     VSVDGLIYNFNYEARFDSSMFR"
BASE COUNT         2482 a         2305 c         2494 g         2492 t
ORIGIN      
        1 aggctggcgg ccagagttat ccgctggatg tctacattgt ttatgaagac gaatcgggat
       61 ccccgatgac gtcaaagatg acaaccatcg ggatacctgt ccatggcaag atcagctttg
      121 tggtaacagg aaatcccaga ccgattatcg cgggagataa acaggtaatt gaggtgatct
      181 atcagaacac aggatcaggg acagcataca atgcccagtc gcgtatcagt gcggtggatc
      241 cgttcaccag taacgacgat accgcgttcc ttggtgatct tgcccccgga gaatccgcag
      301 ttgcccggta tgagatgagc gttgataatt cagcaacccc gaaagagtac ggtctcgatt
      361 ctgaaatacg atacaaagat gcccttggaa acagtcagat ctccgatacc atgaaagtgg
      421 aagtaaccgt tgaagagacc tccccagtta tgacgatcct gctcatttcg gtagtgatcc
      481 tggctgtaat agcggcaggt tattatttct tgatagtaaa aaagaagaat taatgcttgg
      541 ctggtccggc ccgctgcctg aacctgaaat aagggatatt gaggatatgc ggcaggtgct
      601 ggccactcct gactgccagg agaccggccc tctttatttt atgtaccgcg atctagccct
      661 gaatgactgt gacaggcagt ggctgcactc acaccacctg cgatatgaca ttaccgcgat
      721 tgtctcacgc gatatctgcg gcgaacgggt gaagacaaaa gggcattatc atcccttgtc
      781 cccctcgggg gtggggtatc ctgaggtcta tgaagtcctc cgtggtcatg cacattatct
      841 cctccaggag agaaatcttt tgaatgttat cctagtggat gccctggaga aggatctcat
      901 aattgtgccg cccggatatg gccatgtcac catcaatgcg ggaaacagtg atctcatcat
      961 ggccaatatc gtctcaacag attttgagag ccagtatatg gagtatgaga agatgggggg
     1021 tgccgtgtat tatgaactta ccgatggcag gttcataaaa aatccggcat atccatctgt
     1081 ccctgagatc caggtcgtgg aggcaggaca cctgccgaag gtgcattttt ccaaggatgg
     1141 tctttatgag acgatcggga ccgacacgct cgacttcctg aataatccag atctgggttg
     1201 ggatctcttc aatgaagtgc taagaggtta ggtggattcg cccgtttgtg gctgcttttt
     1261 ttcacaaggg atagtacctt ttcttcaacc aggttggtgc tcacccagct gttcgtttcg
     1321 agcgctttca acgccatgtc tatctccgaa taggtgagcc cgatatcagc ttcatcactc
     1381 tgccctgccc agaggcctgc ggagggagtt ttgttgatga tcggttcagg aatccccagt
     1441 tcatgtgcat acttgaaaat ttctgttttg tacagatgga gaatcggttg gaaatcagct
     1501 gcgttatctc caaatttggt acagtatccg agcaggtatt cggtcctgtt cgatgtgccg
     1561 catacaaggc ggtgctccct gtttgcatga taatagagta tcaccatcct ggttctcgcc
     1621 atgatgtttc cgagaagata cggatcttcc acatacccgg gcatctggcg gtatgatgag
     1681 aggacaggtt cgatgctcat aacctgatga tgcatgccga ggctcctgca aaggtcagct
     1741 gcatcatgga tgtcctcatc tcttgttatc gaggtggtga gagtgtagcc atacacccgt
     1801 tcccctccga gtgccctgca gcacagcacc gcagatacag cagaatcaat ccctcccgat
     1861 ataccgataa cgatacccgt ggcaccactc ctccagataa cgtagcggat catctgctcg
     1921 atcttcccca gaaaacatcc atgatcagta tccatagttg gctcctaaat gatccgcatc
     1981 aactctttga tatcagttgg atttcccact gcgatgatgg catccccccg catgagcatt
     2041 tcgtgcggtt gcggtttgac taccgatctc gcctctccct caataccaat gatgcgtaca
     2101 cctgtcttcc tctccactcc cccaatattg tttcctgtac catctcctac cgattttctt
     2161 acgacaatct tgccgtccgg gaggttcagg ataacatgca cgatcgctga aagaaccacc
     2221 cccccgataa tctggccgcc aattgagggg agaagtgcaa catagtcagc cccagcccgg
     2281 tatagtttat gtaccgattc cggctcattg gcacgggcaa gaatcctgat ttccggattg
     2341 agattctttg caatcagtgt ggtaaagata ttcagtgcat cgtcattaag ggcgacgatg
     2401 aaatatttcg cgtctcttat atgtgcctcc agaagcgttg tttcgtcttc tgtatgtcct
     2461 atcacttggg tgattccgtg ttttttcctg tcaattgcaa tgcattcgat gccaaggctg
     2521 gtgagttcgt ggtaggcaga aagtccgaca tcgccgaatc ctccaatcag agcccgctcc
     2581 ctctccctta catgaggcat aaattcctgc ctgaccatct cctccacctg gttgaggtga
     2641 cctataagaa agagcattgt ggtggaatcg agccgttctt cgccggtggg gtatagccgg
     2701 aaattacctt tacgtaagag gagcagcgta aaaaaaccgt accggtcaaa aagatcgagt
     2761 tcctgaagtg tttttccgta agcctgggaa cccggaataa ttgggaggtt gatgatgcgc
     2821 aggcggtttt cttcttgtgg agagtacctg ggaggcatcg tcccattgat cgtctcttcc
     2881 accatcgtgt cgatatgcgt ggtgagtgct gcatgccggg cgattatctg gccagtgaca
     2941 tgttttgcgg acagcacata atctgcacct gcatatttga gatagcgtcc aagcacgagt
     3001 ttatctatca ctgcaatgat tgtcccagat gccatttccc taataccaag aatgatcgca
     3061 gcatttgtcc tctcgtcttc gcatgctatg atggaatggg catttttgac ccaggcattt
     3121 gaccatagtt cgggatcgtt ataatcaccc caaattacgt aggccttagc acccgattcc
     3181 cgtcctgcga gacgcgctgt gtcgggagtg ttcaccacaa taagaaccgg gagatccgat
     3241 atccggatgc tctcgaggag agcttttgtc agttcaccat atccgactat cacaacatgg
     3301 ttataaagtg catgggggag ttttcttggt ggcgctgaaa gaaaaatagt gcttaaataa
     3361 ggcacgagaa gaaggggaat tatcatgaaa ataaggagga tccctgtgat catgacaatt
     3421 atagcaaaaa ggatagtcag ttcattgctg aatgggagga gatccccata gcccactgtg
     3481 gtcactgtct caaggataaa aagcagggaa tttggccagc ttatcggctt tccttcaaga
     3541 acaggatacg caaaatgaaa aattgaggtg aatgcgataa tctggatggt aaagaggaag
     3601 aagaagagcg atatctttgc cctgcgggat gcatgaaagt gtttccagac cattcccctc
     3661 ctgctgtcag acatgatgca gctcccgcaa cataccccat tcctggtatt ctccatgaca
     3721 gggttctcca cagtcgggac aaattcctgc atcaggtagt ggcatcactc catatgagac
     3781 caggtgttcc cccaggttta cgagcgcatt ttttgtggac ggatgtcgcc agttgtactt
     3841 attcaggaga gaatgaacat cagtttctag ggtattgtgg gaataaggac atcgcctgat
     3901 ctcaaatccc cccatattga agtatgcata taatgcaacc tcccttgcag ggatatagag
     3961 gaatgggcga atcattggca cgactctgaa aagactatct gccggtttta gaagtcgttc
     4021 agcatctccc ctgaaaatgt gtaccagaag attcttggct tcgtcatcca gattaagccc
     4081 gagtgccagc tttgttatgt catggtcatt tgcaatctgg ttgaggacta ggccttgtaa
     4141 ccccttacaa aatatgcagg aaaaacggtc tcctttttcc ctgactactt catcaattgt
     4201 cttccctaac acttcatcaa aggaatctgt tattaattca acgccaatgg attcagctat
     4261 gtttgcagtg atacgacggt ccctatatcc tttgattccc tcatcgatgg atatggcaaa
     4321 gaggctgaca tccctccgcg tgcccaggat ttttttcagg aagtacagga gggcactgct
     4381 ctcttctcct ccggacaatg caacagctat cctgtctttt ggcttgatcc agcgatgatt
     4441 cctgatggca cgttttgctt tcgactcaaa atcggcaatg aaatgggctt tacaaagatg
     4501 aagccctgaa tatcgctggt aaatgattgc aggatgacgg catttgctgc agagcatgag
     4561 agggtatcag atcaaccttt atcacagtat agataaattt gtgttgatta catggcaatt
     4621 tcagccgacc ggatcaggga actccataaa tatgagatcc gcatcctgct catccttgag
     4681 cggctgatgc gccgctaccg ctgggttccg tttgacatca tcaagaaaac attaggcctt
     4741 tctgagtcag aggtgaatta ccgcctgggc aggctgatgg ggtgggggtt tgttcggttc
     4801 gataccgttc cctacgaggg atattcactg atattccagg gatacgatgc actggcattg
     4861 atctccctca caaaaaagag cattatccag gcccttgggt gcatgattgg ggaagggaaa
     4921 gaggcggtga tctatgaagg tctggggctg tccccccttg ccctcaaatt ccatcatgtc
     4981 ggccagcgat cctttcaatc tgccagggta acccgggact atatccctga agaaggaaat
     5041 tgcccctggc tccttgcctc acgtctctct gcagaacggg aatacgaagc cctccgtacc
     5101 ctccaccccg atgtcagtgt cccacttcct gtcgatatca accggcatac cgttgtgatg
     5161 gaatttatac ccggggccac gctcaaccgg tgttcactgg aagaccccag ggatgttctg
     5221 cttgagatca ttgaaaacgt aggaaaagca tacgaattgg gcgtcatcca ttcagacctc
     5281 tctgaatata atgtgatgca cgatggcttc cgctgctatc taatcgattg gccgcagtgg
     5341 gttagtactg atcataccaa tgcggaagag cttctgacac gggatcttga gaatattaca
     5401 cggtatttca gccggaggta tcgtgttgag atccccttca tcgaagcgta ccagatggtg
     5461 acgggatgag agtgtttggt atcgatatta taaaaggctc cgtccgctca aaatcacgtc
     5521 gtccgatgta tgccctgata cggatggagg acggagatat cctcggagag ggtgaagtta
     5581 ccggtttccg gctcttcagg atactctctg aagagaaacc ggatattctt gcagtggaca
     5641 gcctgcagga gatagcgatc gatcagcatg atctttatac ctttctccag gctcttccac
     5701 catcggttcg tctcgtccag gtaacaggcg gggaacgcaa ggagagcctg gggaaggttg
     5761 ctagcaggtt caatatcagt ttcaaccgtt ttgatccttt cgatgaagca cggaccatcg
     5821 caagagttgc cgggctgggt gcgggggcgg aggtagttgc attcgagaac accagtgaga
     5881 tcatcgtaag caggcaccga tcgcctggga aaggaggatg gagccagaac cgttacgtcc
     5941 gaaagatcca tggatcggta cagcagaaag cacgggagat tgagatggca cttgttgcag
     6001 cagggctccg gtacaggaag aaagagacac gggcttttgg cggtaacagc cgggtggcgt
     6061 tcgaggtggc ggcggcccgg gatcaggtgc ctgtctccac ctaccgtggg gctgatgtgc
     6121 aggttcggat cagtggcaag aggctcgacc ggattacgtt cagaccgctt tcaggaaaac
     6181 cccgctacct gattgtggga atagatcccg ggaccacaac ggcaattgcg acgcttgacc
     6241 tggacgggaa tcttctggct ctctcaagtt ctcgccagat ggccatgtcg gatgtgatcg
     6301 aggcaatata tcgtgtcgga aaaccccttg tcattgcatc tgatgtccac gagatgccat
     6361 tttctgtcga gaaggtccgg agagcattta atggaatcgc atattccccc cgccaggata
     6421 tgagcgtgga gacaaagcat gagttgactg ctcccttccc ctctcagaat gatcatgaac
     6481 gcgatgccct tgctgcggca gtggaagcat tccggagtta tcggaacaag ttccagaacc
     6541 ttatcagaag ggtccctcct ggaaatgacc tggatgaagt gaaggccggg atagtgaggg
     6601 ggcagtctct ggaacaggtg ctctcggaga tgaaaggcag gccgatccgg cccgttgttg
     6661 aggcgccgaa gatggagatc gatcccagaa ccgatgaacg gatacggatt ctggatggca
     6721 cggtgaagcg gctgaaaggc gttgtaaagg acctccagca agacctccag aagaaagatc
     6781 acgagatcat acgtctccag gggaggatca agaaaatccg gtcgcaacag gatcaagtat
     6841 tccgtcgaga tgcagaaatt ataaagcgcg atgcaattat tacaggcctc aaaaagagga
     6901 tccgccgtga ggagcggaac agcgaaaagt tgcggaaacg aatgaaaaag atccgggatc
     6961 acgatgaggc cggtcccgga gagcaggagg tcatgctcaa actcctgcca tccctcaccc
     7021 gtgaagggat acgagctctc tctgatgaac tggggataaa ggagggcgat ctgatctatg
     7081 tacccaggat tgattcctgg ggtaagaacg cggccaggga actcgcaggt tcaggaatcg
     7141 gtgggctttt agcaagattg ccacggtcat cccggttcga tccccaactg gaagcgatat
     7201 tcagggatgt gtccattccg ctcctctcgg ccgagatgac cggtgttcag gtgaaaggag
     7261 ggatggtcac cgtacctcgg gacaggctgg atgcggcaat cagggcatgg gatgatcaac
     7321 agagagcgta tgaatgggag aagaagaaaa cactcctcga agatatttac cgggaatatc
     7381 ggacagaacg gggaaaggag atgaagaaag ttggatgaaa gggccttaaa ggaggttgtc
     7441 cgcgagatta ttggaacaga acagaccctt gatgattgtg ccgtgttttc tttcgggggc
     7501 atgctcctcg ttgcaacaac cgatatgctg catgaaacca ccgattttcc gggtgggatg
     7561 acggactggc aggttgggtg gatgagcgtt gccgttacct taagcgacat tgcagctatg
     7621 ggcgctcagc cagcgatagt cctcctcgca attggccttg ataatggtga ccgccttcgg
     7681 gagatccttt gcggtgcaaa ggcctgttgt gattccttcg gagcagaact ttccgggggt
     7741 gaccttgatg ctcatcagga actcaccgtc gtcagctcag gaatcgggat cgtggaagaa
     7801 ggagcggtca ttcgccgaag aggggccgtt cccggagatc ttatctgcat caccggccct
     7861 cttggccagg ctcatgctgc gcttgaagga tatagccagt atactcaaaa tcttctcgag
     7921 ccgattcctc gtgtacctga aggaaaaagg ctcgcagcag ccagggtctc ttccatgatg
     7981 gatatcagtg atggactggt gctctcgcta tacgatcttc tcgatgcgaa cccgtacggg
     8041 tactctatcg attcttttct ccttccgatc cctgagggga taccccctga gaaaagtcgg
     8101 gagtatgcca tttttggtgg tggcgatttt gagctgcttt ttacctgttc tccagactat
     8161 ttccctgtac caggtgttga cagtatcatt gtaggggaag ttatcccgga gcatgaggtg
     8221 ctggtggatg gaatcgtggc tccctgcagg gggtatctgc atcggtggaa atgaaaaact
     8281 acgaaagcga tacgatcatc tcatgatggc tggggaaagt atataatttt tcacaggata
     8341 cttagtttta ctacaggtag atactatgaa gaaaagaatt tttgccatcc tggcagcaat
     8401 tgtgctgggg atgttactcg ctgctcccgt atcagcatat tattcaatat cggtaactgc
     8461 aggagctcct gacttaggga acatcatgtc ccccactgtg aaatcattgt taagccagta
     8521 tcagtctccc aggtcgctgt ttggatcgag ctccagtgat ctcgcggcaa acgcaatgaa
     8581 aaacgcagca cagccctcaa aaggctctgt gtcagcgtat agtagtggaa tatccgagaa
     8641 ccaatattca aagatcaact tcaacaaccg tgtttctgtc gatggattaa tctataactt
     8701 caattatgaa gcacgctttg attcaagtat gttcagataa tcagaaaatt ttttgtggtt
     8761 gattggtata ctgacatcaa ttttaatcat ccttcctaca ttttgataaa tcccggtcct
     8821 attcgttact cttttcgaac actacgaata ttatctccaa agagatgaac atctcatgtg
     8881 taaccgtgaa aaatattgga gaggattcaa tgaattctta ctcttcaaac aaaaaggttt
     8941 cacttactta ttcatactac cctcatgagt tcctttgcat ctcttgcttt ttataagcac
     9001 ctaccccctg ctaggaaatc tcggaaaaac ctaaccgatt ttcatttcct catcagatag
     9061 gaggctttcg ccctgtaatc gggggtgtgt ctaactaatc atggaaaaag gcagccatct
     9121 gaatagtgat ttgttgtgat ggctacaagc ctggttcttc aatcgctatg aaatctgtta
     9181 gaaccagaac gataggaaca ggttcaaaat gagatcacat tcagaaatgc gtcggatttg
     9241 gagaatatgt attggctctc aaacgttttg agatgcaaag aacgactgat tcagagagga
     9301 aaggagatgg aattccgggc tgcattttaa cgtcaattac acaaacaagg atggccgatc
     9361 aagaacgaag gtatccttga tgccctgctg acctttcctg gaccgggcaa atcgaaacgg
     9421 tgaccaatca accgggaaaa acgctgccaa tagtaagata attatatgcc ccctgtttca
     9481 ggtaatcgct catgaattta gatctcagaa cagcccttgt ttgattgaga gggatcaatt
     9541 ggttggatta gactgtaaaa acaagaaaat aaaaagtttt catcctggga taatcccagg
     9601 attgatgtta aagatgtaag gttatgctct cttcgttcca gagatccagt tcatgttctt
     9661 gtcgaacagc atgatgtatc catcaacact ggttctctct tcgaattcaa cctttgcgta
     9721 catcttctca tcgccgccac ggccttccat gatcaatccc ttcatgtagg cac
//
LOCUS       DAOOCO010000056         9427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6897_length_9427_cov_5.291613, whole genome shotgun sequence.
ACCESSION   DAOOCO010000056 DAOOCO010000000
VERSION     DAOOCO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_9427_cov_5.291613"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..493)
                     /locus_tag="PK154_04580"
     CDS             complement(287..493)
                     /locus_tag="PK154_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HPW10370.1"
                     /translation="MADLPVAAIVRIAKKSGAKRVGRDAAMTLSAKAEDYIAQLTKEA
                     NKLAIHAGRKTLKEEDVELAAMSA"
     gene            907..2406
                     /locus_tag="PK154_04585"
     CDS             907..2406
                     /locus_tag="PK154_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HPW10371.1"
                     /translation="MTKLCQKMIDEAMAAQRADVDTVKKKRGGSFVVSDAKSYLSAVK
                     KMKPAGAQSKAVFGLHTDSVKAHFGVLEQLTSTVRPEDDPYVEHYQTPVIMEILYDED
                     PSFLKSVKAFTRAIRKAEALVGKEAARRYAGFYGPTCVVDFALIPGSTSNVVNQILKT
                     VDIPTPHKQAILASKSWGMNTSYGFGDVFAHEVEKGATLADAVKAEIGMMEKVYDRPV
                     DAQADLMDSVGMTSFDARKYMEGYRKKMEGTVRAAMDDDVHYANIVTIPAYCVGDIAH
                     HIAQSTFNMCKDDVIMATIEAVTDVMDATLKAAVPSFKTEEDVLTVATGASASAAEYI
                     LELDGFNAIMLVDLLTKRWHNYVQLYPKRGAAAELHNCDFMDMLYRGWKYVDQARRRQ
                     NGTDGLLKPKVGGVVVDLQPVHENEVLMNPQRYAYPACAITVRASAMLRLSDYPCLLT
                     SEPVTATLMTNIIALKKDVPAAPARICKNCASAALIDFRHQYCQWKCAV"
     gene            2420..2629
                     /locus_tag="PK154_04590"
     CDS             2420..2629
                     /locus_tag="PK154_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HPW10372.1"
                     /translation="MKCYICAQMGKSTDAVAICIICGMGTCMEHTIRREVDVWEGGYP
                     FPAKKLPKKIPRMLCPDCNASYKEG"
     gene            2633..3370
                     /locus_tag="PK154_04595"
     CDS             2633..3370
                     /locus_tag="PK154_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPW10373.1"
                     /translation="MSSLARRCCAEMLGTGLLVYFGAGAAAMTLMIAAGTDPATPFNI
                     GIGALGGLADWLAIGMAFAIVIAAAIYALGSVSGAHLNPAVSIALWATKRFPAGDTGA
                     YIVSQLVGAAIGSLLFAASAGMDAVTIGGLGATAPFPGISYGQAILAEFIGTFLLMLV
                     IMGVAVNSKAPPGFAGLVIGLTVGGVITTTGNIAGASLNTARTFGPYLGDWLLGGNNF
                     WVYFPIYVIGPILGAVTAAFLYDYLTG"
     gene            3722..5185
                     /locus_tag="PK154_04600"
     CDS             3722..5185
                     /locus_tag="PK154_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10374.1"
                     /translation="MMKTMLVLFTIALFVALPVSATVIPSSIEATLWPGEFVGENKTV
                     EVTALPAKADVIFAFDCTGSMGGTLADAKANAADVMAALELTGVDIQYGVMSHRDYPG
                     YFDSCDYGGTYGEVDDWPYLLEQAITDDTTDIQTAINGLSAGGGWDGPESYSRLLYET
                     YSDPNVGWRIGAKRIVVSFGDVVPHDCDMSCLGYTESTGIDPGRDATLFTADDLAILD
                     VIDGMADANIILLEVQPYDYYQPCWDPWVATTGGSFWVLGGFEVDDMVDVIISGLTTP
                     NVCGLTMVAESGFESWLTSVVPESYDCFEPPATMEFDITITVPEGTECGDYTFTVSAV
                     DEDGVSYGDQEVTIHVPCVLPVDVDFKPGSCPNAFNRGEKGVLPVAILGSDTVDVSQI
                     DPETVLLEGVAPIRWSIEDTGAPVPCDDECQPCECWQGLGDGYLDLNLKFESPAIAAT
                     SAVTGATVKGDPVPLAITGELFDGTPISGGDCLWIVK"
     gene            5650..6318
                     /locus_tag="PK154_04605"
     CDS             5650..6318
                     /locus_tag="PK154_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10375.1"
                     /translation="MRAYMRVADYLGKFWTGAEVMYGVIIAMTFTSMLRGVPFILQGI
                     LTKIVLAALFCCIAWGIADGLFYLWERNYIIRRENKTIELSKRGEEKEPALTLVGEQL
                     DDTILRNIPPENRLHLYEKLVQFLSTVQEREKFSARGALTIVIGTFLLSAGAGLIVVA
                     PFFLIDNVGQALNVSNLCGILLLFVVGYTRALERTFLSKIMMGFASSCIGILISAITV
                     ILGG"
     gene            6486..6797
                     /locus_tag="PK154_04610"
     CDS             6486..6797
                     /locus_tag="PK154_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10376.1"
                     /translation="MNRDDIIREVGLEPWVLPGRTYPTPLPVDLLPFYCYTRDGGHSL
                     LVVVENEYRQGLSPVRFIIPAPVKLVLKAGYHLHDGLLWARLPYDRDEGLRVDDSDVE
                     Y"
     gene            7407..7745
                     /locus_tag="PK154_04615"
     CDS             7407..7745
                     /locus_tag="PK154_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10377.1"
                     /translation="MEKAIVSISQEAEYFFRRAMDAEMKNNPVQVLEYFNKALSKDPG
                     YAMAWNEKGNYLDLMGKLDEALSCYDTALKLEPGDAEIWFNKGLTLKKVGREEDAVSC
                     INRGIELAIG"
     gene            7767..8105
                     /locus_tag="PK154_04620"
     CDS             7767..8105
                     /locus_tag="PK154_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10378.1"
                     /translation="MTKFGTILHEAEYYYQRAIHSQDKEKPEKILEYFDRAIAAHPGY
                     AMAWNEKANFLDYLGKCEDAISCYDMALKIDPQLSEAWFNKGLTLKKMGKEREGIDCI
                     NKGIDFACGR"
     gene            complement(8212..8955)
                     /locus_tag="PK154_04625"
     CDS             complement(8212..8955)
                     /locus_tag="PK154_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW10379.1"
                     /translation="MNVTFLGTNGWYDTLTGNTCSVLVQSDDWNIIFDAGNGIAKADR
                     YIDQGKPTVLFLSHCHLDHLAGLHTLTKFRFRHPLQICGQPGTEALLRSIVREPFMVP
                     FEDLPFPVGFTELQEGNHRFPFPVECRPLVHPVPCYGYAITLEGRKIAFCTDTGFCEN
                     AVILSKDADLAITECGLRPGEESPDWPHLNPEQAIRIAREADAKRLALIHFAAHTYTS
                     LKDRSAIEKRYRKEFPGLIATRDGLTVLV"
     gene            complement(9077..>9427)
                     /locus_tag="PK154_04630"
     CDS             complement(9077..>9427)
                     /locus_tag="PK154_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW10380.1"
                     /translation="EWRGPEQMGLYRAVFGDEGAMQRGYQDYCFPINILHDAGIFANV
                     RIYPALWETSYESVDEAVRNWVTMHHPDLQDQTPVTEFFTRVLRKGATGRLKYSAVRR
                     TAVIWWEKEADGKA"
BASE COUNT         2183 a         2444 c         2573 g         2227 t
ORIGIN      
        1 taaaggcaaa gaagcaggaa tacccggcaa aatccctgct cgtcatgaag gtcaccgacg
       61 tcttcacctg cctcccgggc gctgaagccg ggaagaagct gctatagacg gggaaacagc
      121 tcctttcccc ttattcatcc acactccagg gacctgttcc aatcgcatga agggacagaa
      181 gatcctgatt ccgaggaaaa ggaccgggat gataaaaaat catcatcctc gtagtaaaat
      241 cgcaaacagt gtgatattga gaggagaaga gaaaaaagcg ggagagttac gcggacattg
      301 ccgcgagctc cacatcctct tctttcaggg tctttcttcc ggcgtgaatc gccagtttgt
      361 tcgcttcttt tgtcagctgt gcaatataat cttcagcttt tgcagaaagg gtcattgctg
      421 cgtcgcggcc gaccctcttt gcaccacttt tcttggcaat tcttacaatt gccgctacag
      481 gtaaatctgc caccttaatt acctcaaaca gataaacatc ctctaaatat ttatactttt
      541 aggaagccgg ccggatcctg gggctggatc ttcttttcag gctattgtga aggatatatg
      601 ttctatccct ccctgttatc ccgttcttca ggaggatgca ggtatgttct cctgctgctg
      661 cgaaaagcaa gggaagatag gtctcggggc ttctgcccct cctttcgcat accggcagtt
      721 gcactcctcg tttcatccta tggtaccagt ccaaaccttt atccgtaatc cggatcgatt
      781 ctggctttag caccggagat gccagtcatg gctgcaatct ttgccggatc agcattccct
      841 ctccgggctg gcgtagtcgg atccattcct ggacaaccgg ctcctgattc atggaggtgt
      901 tctcatatga cgaaattgtg tcagaagatg atcgatgagg ccatggcagc acagcgggct
      961 gacgtggata ccgtgaagaa gaaacggggg ggatcgtttg tggtctccga tgcaaaatcc
     1021 tacctttccg cggtaaagaa gatgaaacct gccggggcgc agtcaaaggc tgtctttggc
     1081 ctccataccg actccgtgaa agcccacttc ggggtgctgg aacagctgac gagcaccgtc
     1141 cggcctgagg atgatcctta tgtcgaacat taccagactc cggtgatcat ggagatcctc
     1201 tatgatgagg acccttcgtt tctgaagagt gtcaaagcgt tcaccagggc gatccggaaa
     1261 gccgaagcgc tggtgggaaa ggaggcagcc cgccggtacg ccgggttcta cgggcctacc
     1321 tgcgtcgttg actttgccct gatcccgggc agcacgagca acgtcgtcaa ccagatcctg
     1381 aagaccgtgg acatccccac cccccacaag caggccatcc ttgcctccaa gtcctggggg
     1441 atgaacacga gctatggctt cggcgacgtc tttgcccacg aggtcgagaa gggggcaacc
     1501 cttgccgatg cggtgaaggc cgagatcggg atgatggaga aggtctacga ccgcccggtc
     1561 gatgcccagg ccgacctgat ggactcggtc gggatgacct cgtttgacgc acggaaatac
     1621 atggaggggt accggaagaa gatggagggg acggtccggg cggcaatgga tgacgatgtc
     1681 cactatgcca atatcgtgac catccctgcc tactgtgtcg gggatatcgc ccaccacatc
     1741 gcccagtcca ccttcaacat gtgcaaggac gacgtgatca tggccaccat cgaagccgtt
     1801 accgatgtga tggacgccac gctgaaggca gccgtcccct cgttcaaaac cgaggaagac
     1861 gtcctgacgg tagccacagg ggcctcggct tctgctgccg agtatatcct cgagctggac
     1921 ggctttaatg ccatcatgct ggttgatctc ctgacaaagc gctggcacaa ttacgtccag
     1981 ctgtatccga agcggggggc ggctgccgag ctgcataact gcgacttcat ggacatgctt
     2041 taccgtggct ggaagtacgt ggaccaggca cgccggcgac agaacgggac ggatgggctg
     2101 ttgaagccaa aagtcggcgg ggttgttgtc gatctccagc cggtccatga gaacgaggtg
     2161 ctgatgaacc cgcagcggta tgcctaccct gcctgcgcca tcacggtcag ggcatcagcc
     2221 atgctccggc tctctgacta tccctgcctg ctgacgagcg agccggtcac ggccaccctg
     2281 atgaccaata tcattgccct taaaaaggac gtcccggcag ccccggccag gatctgcaag
     2341 aattgtgcct cggcagcgct gatcgatttc aggcaccagt actgccagtg gaaatgtgcg
     2401 gtgtaacgga gtgagtgaga tgaaatgcta tatctgcgct cagatgggga agtccaccga
     2461 tgcggtggcg atctgtatca tctgtggaat ggggacctgc atggagcaca ccatccggag
     2521 ggaggtcgac gtctgggagg gaggataccc gttcccggcc aagaaactcc cgaagaagat
     2581 cccgcggatg ctctgcccgg actgcaatgc ctcatataag gagggatgag ggatgagttc
     2641 cctggccagg cggtgctgtg ccgagatgct cgggacaggg ctgctcgtgt atttcggtgc
     2701 aggtgctgct gccatgaccc tcatgatcgc agcagggacc gacccggcga ccccgttcaa
     2761 catagggata ggagccctcg ggggccttgc cgactggctg gcgataggaa tggcgtttgc
     2821 catcgtgatc gccgccgcaa tctatgctct cggcagtgtc tcgggcgccc atctcaaccc
     2881 tgcggtcagc atcgcgttgt gggccacgaa acggttccct gccggggata ccggtgccta
     2941 tatcgtttct cagctggttg gggctgcaat cgggagcctg ctctttgcag caagcgccgg
     3001 catggatgcc gtcacgatcg ggggtcttgg ggctactgct cccttccctg gcatcagcta
     3061 cggacaggca atccttgcag agtttatcgg gacgttcctg ctgatgctcg tcatcatggg
     3121 agtagcggtg aacagcaagg caccaccggg atttgccggg cttgtgatcg gcctgacggt
     3181 cggcggagtc atcacgacga ccggcaacat tgcaggcgcc tcactgaaca cagcccggac
     3241 gttcggcccg tatctcggcg actggctgct tggcgggaat aatttctggg tgtattttcc
     3301 gatttatgtg atcggtccga tcctcggagc ggttacagcc gcgttcctgt atgattacct
     3361 gaccggatga atcctttttt ttaaaacctg gatgagcaga agggatcaat ctttggttca
     3421 tggatgtgat ccataacagg agaaataaac attttaaaaa attccattgt taacaaaatt
     3481 gcatggcata tacataaata tccatatcag tgcagcagaa actacggaag tcgagttgga
     3541 gagaggcata ctctgaagtg gtctggattc atctgatggt gcaaggaaaa gatcagtagt
     3601 caatgaaagg ctgacttccg tttgagaaag gtggtgggtc aagcgagaac tctttccgta
     3661 gttgtctgtc cgtagctgct tcagagaaat tttaatgcaa gaattggaga gatggtgcgt
     3721 tatgatgaaa actatgttag tactcttcac cattgcactc tttgtggcat tgccggtgag
     3781 tgcaacggtc ataccgtcat caatcgaagc gactttatgg cctggtgagt ttgttggtga
     3841 gaataaaaca gtcgaggtta ctgctcttcc agccaaggct gatgtgatat tcgcatttga
     3901 ctgcaccgga agtatgggag gcactctcgc tgacgccaag gcaaacgcgg cggatgtcat
     3961 ggcagcgctt gaattgaccg gggtcgacat tcagtatggt gtaatgtccc acagggatta
     4021 tccagggtat tttgattcgt gtgattatgg tggtacttac ggagaagttg atgactggcc
     4081 gtatctcctc gaacaagcaa taaccgatga cacgaccgat attcagaccg caatcaatgg
     4141 cttatctgct ggaggaggat gggatggccc cgagtcctat agcaggctcc tgtacgaaac
     4201 ctatagcgat cctaacgtgg ggtggcgtat tggggcaaag cgtattgttg tctcctttgg
     4261 ggatgtcgtc cctcatgatt gcgatatgtc ctgcttgggg tatacggaat caactggtat
     4321 tgatccgggc cgggatgcaa cgctatttac tgcagatgac cttgcaatac tggatgtcat
     4381 tgatggtatg gcagacgcga acattatcct tcttgaagtt caaccctacg attattatca
     4441 gccctgctgg gacccctggg ttgctacaac tggtggaagc ttctgggtcc tcggaggatt
     4501 tgaagtcgat gatatggtcg atgtgatcat ttcaggactt actaccccta acgtctgtgg
     4561 ccttaccatg gttgcagaat ccgggttcga gagctggctc accagtgttg tccccgagtc
     4621 gtatgattgc ttcgaacccc cggctaccat ggagtttgat atcactatca ccgttccaga
     4681 gggaactgaa tgcggtgatt acacattcac agtgagtgcc gttgatgaag atggtgtcag
     4741 ctacggggac caggaagtga caatccacgt gccatgcgtt cttcctgttg atgttgattt
     4801 caagcccggg tcctgcccga acgcctttaa ccgtggtgag aaaggggtcc tgccagtagc
     4861 gattcttgga tcagacactg ttgatgtgag tcagatcgat ccagagaccg tgctccttga
     4921 aggagttgcg ccaatccgct ggtcgattga agataccggt gcacctgttc cttgtgatga
     4981 cgaatgccag ccatgcgaat gctggcaggg attaggggac ggataccttg acttgaacct
     5041 caagttcgag agcccggcta tcgcagcaac cagtgcagta actggagcga cggtaaaagg
     5101 agaccctgta ccacttgcca ttactggcga actctttgat ggtacaccga tatcgggagg
     5161 agactgtctc tggattgtga agtgatgaat acctgtggcc cgtcgccctg ggccacaagc
     5221 actttttttc aatttgattc ggaactggga gaaactttcc aaaatcagat aactggctcc
     5281 tatttcatgg tttgccctcc gatttttaaa aattagttcg aatcaaataa aataaatatg
     5341 aaaactcaaa agtaatagag agcggtgcag attaacccaa gtggttaatc gctatcgtga
     5401 tatctcatca atgaggtttt ttaaaacctt atcatcacct gtaaaggagg ttctgaccga
     5461 acctcaccca ctccttattc ccggtgcctt tacagaaccg tttgaattat gctattgttc
     5521 ctgatatccg atacctgaaa acaaccaata cattttccat tcttattttt tggttgatac
     5581 cctcctccct gctctggacc ctgtcctttt taagatacct taataaagga atgacattca
     5641 ggctatctct tgcgagcgta tatgcgggtt gccgattacc ttgggaagtt ctggacgggt
     5701 gcagaagtga tgtacggggt catcatcgcg atgaccttta caagcatgct caggggggtt
     5761 cccttcatcc ttcagggaat cctcacaaaa atcgtccttg ccgcgttgtt ctgctgcatt
     5821 gcctggggaa tagccgatgg cctcttctac ctctgggagc ggaattacat catcaggcgg
     5881 gagaacaaga cgatcgagct ctcgaagagg ggagaggaga aggaaccggc actcaccctt
     5941 gtcggagagc agctggatga tacgatcctc cggaatattc cgccggagaa ccggctccac
     6001 ctctatgaaa aattggtaca gttcctctct accgtacagg agcgggaaaa gttctctgcc
     6061 cgtggagcac tcaccattgt cattggcacc ttcctcctct ctgcaggtgc aggcctgatc
     6121 gtggtcgccc cgttcttcct gatcgataat gtagggcagg ccctgaacgt atcgaacctg
     6181 tgcgggatac tcctcctctt tgtggtcggc tatacgagag cccttgagcg aacctttctc
     6241 tcaaagatca tgatgggatt tgcatcctcc tgtatcggga tcctgatcag cgcaatcacg
     6301 gtcatcctgg gtggctagcg gcaattggag ggacatttct cctggtcgta agacccatcg
     6361 cactctcctt tcattcccat ggtactcatt cccactcctg ccacaacagc atgctcaagg
     6421 cagggagggg aagatcttca ggattatgag ggatggggtc tgaccgttga tctggtagtc
     6481 caatcatgaa tcgtgatgat atcatccgtg aggtaggcct ggaaccctgg gtgctcccgg
     6541 gtcggaccta tccaacaccg ctcccggtgg atctcctgcc cttttactgt tataccaggg
     6601 atgggggaca ttccctgctc gtcgttgttg agaacgaata ccgtcaggga ctctccccgg
     6661 tgaggttcat catacctgcc cccgtaaagt tggtgctgaa ggctgggtat cacctgcatg
     6721 acggactttt atgggcaagg ctcccttatg accgtgatga aggcctccgg gtcgatgact
     6781 cggatgttga gtactgataa tccatccgct ggtatccctt ggtgtcaggg gagaaatccc
     6841 ctgaattccc tgatgcttcc cattgcgatt gagatgaaga ccggaatgaa tatcagacct
     6901 gcccgttatc tctgtcctgt ccctggattc aggggaggaa tccctgaatt ccctgttatt
     6961 ccaaataaga ccgggcagaa ggataagacc gtacacccga ccataccgga ttcggtggta
     7021 ttattccctg cggcacgtga gcttcggcac cacacttcca gatacttttg ccagttcctg
     7081 cccgactttt ttcaatggtg aagttgtggg tatcgaaacg ctgtgttcat gggatgagat
     7141 ccttattccc acagaggttc caattataat accctccgaa tattccccca aacacccctt
     7201 ttcacgaggg aaccgggagg ggtcgttacc gtcgctcagc aatgccatgc actgttgtac
     7261 cgcgaatcta atgcagtagt gcaatgcaca ttattcgaac aataatatac tgcaaactaa
     7321 tatttaagca taagaattat tgttgtttgt cggggaccac acaagacaga tgccctggcg
     7381 gaatcacaga ggaggaagga acaaccatgg agaaagcaat cgtcagcatc tcgcaggaag
     7441 ccgagtattt cttccggcgg gcaatggatg cagagatgaa gaacaacccc gtacaggtgc
     7501 ttgagtactt caacaaggca ctcagcaagg atcccggata cgcaatggca tggaacgaga
     7561 aggggaacta ccttgatctg atgggaaagc tcgatgaggc actctcctgc tatgacacag
     7621 ccctgaaact cgaaccaggg gatgccgaga tatggttcaa caaggggctc accctgaaaa
     7681 aggttggaag agaagaggac gcagtctcct gcatcaaccg gggaatagaa ctggcaatcg
     7741 ggtaacacat acaaggagga agaagaatga caaagtttgg caccatcctg cacgaggctg
     7801 aatactacta ccagcgggca atccattcgc aggacaagga aaaacctgaa aagatacttg
     7861 agtacttcga ccgggctatc gcggcgcacc ctggatatgc catggcatgg aacgagaagg
     7921 caaatttcct tgattacctt ggcaagtgcg aagatgcgat ctcatgctac gacatggcgc
     7981 tcaagatcga tccgcagctc tccgaagcct ggttcaacaa gggcctgacc ctgaagaaga
     8041 tgggaaaaga gagagaaggg atcgactgta tcaacaaggg catcgacttt gcgtgtggga
     8101 ggtaatacgg tctctttttc tcacccattt cgctcccctt cacaatgaag aaccctattc
     8161 tctctcttcg ttcaccctga tcttttcaaa agagcccttc ttggcgctcc ttcagaccag
     8221 gaccgtgagt ccatccctcg tggcgatcag tccgggaaac tcctttctgt acctcttctc
     8281 aatggccgaa cggtccttga ggctcgtgta agtatgggca gcaaaatgaa tcagggctag
     8341 tcgttttgca tcagcctccc tcgcgatcct gatcgcctgt tcaggattca ggtggggcca
     8401 gtccgggctc tcttcgcccg gacgcagtcc gcattcggtg atggcaaggt cagcatcctt
     8461 tgagagtatg acggcatttt cgcagaagcc tgtatcggtg cagaatgcga tcttcctgcc
     8521 ctcaagggtg atggcataac cataacaggg caccgggtgg accagcggcc ggcattcgac
     8581 cgggaagggg aagcgatgat tcccctcttg cagttccgtg aacccgaccg ggaagggaag
     8641 atcctcgaag gggaccatga agggctccct cacaatggac cggagcaggg cctctgtccc
     8701 aggctggccg cagatctgca gcgggtgcct gaaccggaat tttgtcaggg tgtgaagacc
     8761 tgccagatgg tcaaggtgac aatggctgag gaagaggacg gtcggcttcc cctggtcgat
     8821 ataccggtcg gctttggcga tcccgttgcc ggcatcaaaa atgatgttcc agtcatcgga
     8881 ctggaccagc accgagcagg tgttcccggt aagggtgtcg taccagccat tggtcccgag
     8941 aaacgtgacg ttcatctgga tctgattgtg gctgatgagg caaaaaaagg tgcagggtct
     9001 cccggccatc tctttgccgg aacgatgaac catctcccgg gatatcgtaa gagaattccg
     9061 gcagagggaa tcccggtcag gccttcccgt ccgcttcctt ctcccaccag atcacagcgg
     9121 ttcgccttac cgctgagtac ttcagccttc ccgtcgctcc tttcctgagc accctcgtga
     9181 agaattcggt gacgggggtc tgatcctgga ggtccgggtg atgcatcgtc acccagttcc
     9241 tgacagcctc atctaccgat tcataggagg tttcccagag tgcaggatag atccgcacgt
     9301 tcgcaaagat accggcatca tggaggatgt ttatggggaa gcaataatcc tggtatcccc
     9361 gctgcattgc cccctcatca ccgaacactg cacggtagag ccccatctgc tcgggccccc
     9421 tccactc
//
LOCUS       DAOOCO010000057         9382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6934_length_9382_cov_6.081055, whole genome shotgun sequence.
ACCESSION   DAOOCO010000057 DAOOCO010000000
VERSION     DAOOCO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_9382_cov_6.081055"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK154_04635"
     CDS             <1..521
                     /locus_tag="PK154_04635"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPW10381.1"
                     /translation="YDLVREQPVIEFTGMHLKKDFIYHGILPGGNEHKMLMGAPYEPS
                     IYRAVGGVTEVKNVVLTTGGCGYFHAVIQIRKQTQGDGKNAILAALASHTSLKHVVVV
                     DTDINPSDIQDVEFAIATRVRGDKSLLVIPGARGSSLDPCQAEDGTNVKVGIDATMPL
                     GREDEFRRAGWD"
     gene            531..1700
                     /locus_tag="PK154_04640"
     CDS             531..1700
                     /locus_tag="PK154_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HPW10382.1"
                     /translation="MHLLAEEEAILAGESGETRQQMMEILVALGKVFSADRLVPIRSA
                     QVSGASYKTIGEAGLEWLSTLDARVSVPTILNPVGMDRVRWREMEIGQAFARKQEDVI
                     NAYARLGIKLECTCTPYYIYQTRFGDHLAWSESSAVAYANSVIGARTNREGGPSALAA
                     AIIGKTPEYGLHRVEERKPQIVIRIDRATNWEESALYGALGYFAGSEVGDKIPYVQGI
                     RPKRGHLKALGAALAASGAVALFHVEGITPEARIFEYDVGSLEVLSVEPEEILSLMNR
                     IPVDAVALGCPHCSPEELTEIAGLLEGRRVTRPLYIFASRKVIEKSTDAVTCIERYGG
                     RVFADTCMVVSPALEQYGAIMVNSGKALAYVPNMCGAAVRIGTTEECINSATEGS"
     gene            1843..3102
                     /locus_tag="PK154_04645"
     CDS             1843..3102
                     /locus_tag="PK154_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10383.1"
                     /translation="MESFRNYRMPVLVLLVVISILLEIVLHWYLGISVAFSHFFYIPV
                     VLAAVWYGKRAVVVAMILGIAHLAGTYFSIGFIDTVALLRAMMFIVIALVLGAITDRM
                     KKEQEQIINKVTDAAFSAGLNEGSTRGIQGRKSRIPSFASVKTMSERRDIPGLIRALK
                     NRDQAVQYQAAEALGNIGDASATEALMDALTGDQYSGIRWKAAEALGKIGAPAVPHLI
                     QALKNPDEDVRWKAAVTLGEIGDLQSAEPLVDLLGDEDRFVRSRAAYALGLLGSSAVA
                     PLTKALLNGSIEIRRGAAAALGTIRDPNAVEALIQALGDPVDEVWQDVIGALSRLGEL
                     SYHPLVNALRSHERRSLEGAALALAGSGAPEAVGTLTSAIDTADPAIRPVLQSIVNEV
                     MARQNRQSSRNGEELPGFSTKEPSGSS"
     gene            3153..3668
                     /locus_tag="PK154_04650"
     CDS             3153..3668
                     /locus_tag="PK154_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10384.1"
                     /translation="MKMEISRDIKALADPDRNIRIKAVKRLSDIGQPAVEPLIRAMEH
                     EESKDFRWYAAVALARIGQPAIEPLITALKGNQDREFRRYASAALGHMGGEAVDPLLL
                     AMKSADPSMRGFLSAALCRIGPAAIERLKEAYASHDPDLRECASLTLWRMGESGVIGM
                     MQAIERENEGP"
     gene            3726..7586
                     /locus_tag="PK154_04655"
     CDS             3726..7586
                     /locus_tag="PK154_04655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HPW10385.1"
                     /translation="MALQRSGVMESYEKSLQDGLGAALEVATRARAVGIDPRTRVEIP
                     IASDLADRVEALLGIRGIAARIRELESGMSREEAALRIGDDFVERRFGEQSREEILDH
                     AIRTAMAVLTEGVVAAPTEGIAKIGIGKNDDGTEYLRIYYAGPIRSAGGTAQALSVLV
                     GDYVRRALGINRYIPRAEEVERYIEEIRQYNTIMNLQYLPGEREIRLIVGNCPVCIDG
                     EGTEQEEVGGYRNLERIETNAVRGGMALVLAEGLALKAPKVQKYVRAMKMDGWEWLDE
                     LTSGAAKSSDPDSDTIQPKDKYLRDLIGGRPVFSYPMRKGGFRLRYGRSRNTGFAAAG
                     INPATMHILGNFLAVGTQMKIERPGKAAGIVPVDTIDGPMVRLKNGDVLRVDDAETAR
                     ALAPDIDRILDVGEILISYGEFMENNHLLVPSAYCEEWWQLEGGFERPSNELEAIALC
                     CEGAFLHPDYTWFWDDISPGQVAELAGFIEEHGEIRHGVLLLPDEPAIKEILEELLIA
                     HMVREKMICISTNKVLLACLGLDLTLRRRKEWENAPMERSLDLVMHLSGFKIRSKAGT
                     RIGGRMGRPGKSKPRKMNPPPHSLFPLGEAGGSRRSFQEASNHALEANKDGGIIRIEA
                     GRRRCSVCGTETYHNRCQCGAHTEAVYTCQRCKRESLEPECPVCKVPSSCSHHLALNV
                     KQEYLLAMERLGRRENPVGLVKGVKGLISRERAVEEIEKGILRAENELFVFKDGTIRF
                     DMIDLPLTHFRPDEVGVSPERLCELGYASDIKGVPLTRTDQVLELKPQDILISESCAD
                     YMVRVARFIDELLTRCYHLPAYYSVNSRTGLIGHLVIGLAPHTSAGVLSRIIGISRAN
                     VGYAHPFFHAAKRRNCFYGDTELEFFDGNVWRKQQLRQFVIENFDLSRPGIDRLGTYY
                     SDPRQGYLTRTIDREGKPHLRKVTSVSIHKSPPHLISFETTQGRIIAVTPDHAMLVWD
                     LCYLRKIRADELKEGDPVPVMVGMNVITDHIKRRDLVPAPEDRVYCLTVEEEHTVCAN
                     GIFTGQCDGDEDCIMLLLDGLINFSRAFLPESRGGTMDAPLVLTTSIDPAEVDKESHN
                     LDVMASYPLELYLAGLRYAHPREIVSLIDRVENRLGTPAAMEGFLYTHETSDISAGPL
                     ESTYTQLKTMIEKLEAELTLAEKIRAVDQDDVAERVLTTHFIRDLMGNLSAFSKQKLR
                     CVKCNHSYRRMPLAGKCIRCGGNVIPTVHEGSVKKYLEVSRAICEKYKVSEYTKQRVM
                     VLDRAIESTFGHEKDQQLGLADFM"
     gene            7842..7924
                     /locus_tag="PK154_04660"
     tRNA            7842..7924
                     /locus_tag="PK154_04660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7876..7878,aa:Leu,seq:caa)
     gene            complement(8036..8680)
                     /locus_tag="PK154_04665"
     CDS             complement(8036..8680)
                     /locus_tag="PK154_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPW10386.1"
                     /translation="MREKMSRFPASSSPHLRHNASFITTRDGIRFSNIATPYRQITEI
                     PEYWMEDIREDDHILDIGANIGAFCIRAAKKSSHVSAVEPFTTDILNTNITLNGVDIK
                     VFCGALGDGFPTYIEWDGISSRDSTYRLSDLIQRAGGCDFLKCDCEGAEWQIRPVDLE
                     GIRRIEMELHQPPIGGPINTELLQYISEKYAFSIDRDPVHGPLGLFGILHAWQP"
     gene            complement(8733..9104)
                     /locus_tag="PK154_04670"
     CDS             complement(8733..9104)
                     /locus_tag="PK154_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10387.1"
                     /translation="MAAGDVVTAVVWIGGKEVPASSDPDKRLDKTSVGFEVTGYVPDP
                     LTDPGGNVVSNSDIVGDATVGADGEVGDTSLEYSASGLDPGGGKKYSAEIIIPPISSA
                     TTAKSRPNSFWFIPEIVDNGI"
     gene            9124..>9382
                     /locus_tag="PK154_04675"
     CDS             9124..>9382
                     /locus_tag="PK154_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10388.1"
                     /translation="MIVTPVEARAKPGDTILYVMAIEPKGGFDEPVSLRLEVNALLLY
                     RESFDLGDIDPPFPKTIEYRFVVPSEVPGGITVKGHITAEGG"
BASE COUNT         2241 a         2327 c         2645 g         2169 t
ORIGIN      
        1 cctatgacct cgttcgtgaa caacccgtga ttgaatttac cgggatgcat ctgaaaaagg
       61 actttatcta tcatggaatt ctccctggcg gtaatgaaca caagatgctc atgggtgcac
      121 cctacgaacc ctccatctac cgtgctgtgg gaggagtgac agaggtgaag aacgtggttc
      181 tcaccacggg gggttgtggc tactttcatg cggtcatcca gatccggaaa cagacacaag
      241 gagatggaaa aaatgcaatc cttgcagccc ttgcatccca tacctccctc aagcatgtag
      301 tggtggtaga cacggacatc aatccctctg atatccagga tgtagagttt gccattgcga
      361 cacgggtcag gggagataaa agcctgcttg tgatcccagg tgccagggga tcatcacttg
      421 atccctgcca ggccgaggat ggcacaaatg tcaaggtagg gatcgatgcc acgatgccgt
      481 tgggcaggga agatgaattc agaagagccg ggtgggactg aggtacaatt atgcacttgc
      541 ttgcagagga agaagcaatc cttgccggcg aatcaggtga gacccgtcag cagatgatgg
      601 agatccttgt tgctctcggc aaggtatttt ccgcagacag gctggttccc atcaggagtg
      661 cccaggtgag cggggcttcc tacaagacga tcggcgaggc cggccttgaa tggctctcca
      721 ccctcgatgc ccgcgtatca gtaccaacga ttctcaaccc ggtagggatg gatcgggtcc
      781 gctggcggga gatggaaatc ggtcaggctt ttgccagaaa acaggaagat gtgatcaatg
      841 cgtatgcacg gcttggcatc aagttggaat gcacctgcac gccatattat atctaccaga
      901 cccggtttgg ggatcatctc gcatggtcgg agtcctctgc agttgcctat gcaaattcgg
      961 tcatcggggc acggaccaac cgtgaaggag ggccttcggc ccttgcagca gcaatcatag
     1021 gaaaaactcc tgaatacgga ctccatcgcg ttgaagagcg gaaaccacag attgttatca
     1081 ggattgaccg cgcaaccaac tgggaggaga gtgctcttta tggcgcactc ggctattttg
     1141 ccggaagtga agtgggagat aaaatcccct atgtacaagg aatccggccg aagaggggcc
     1201 acctcaaggc tctgggtgct gcattggctg catccggtgc agttgcgctt ttccacgtag
     1261 agggaattac tcctgaagcc cgtatctttg aatacgatgt ggggagcctt gaggtgttat
     1321 cggttgagcc agaggagatc ctttccctga tgaaccgtat cccggtcgat gcggtggcgc
     1381 tcggctgccc tcactgctca ccggaggaac ttactgagat agccgggctc ctcgaaggca
     1441 gaagggtgac ccggcccctg tatatatttg cctcccggaa ggtaatcgag aaatcaactg
     1501 atgctgttac ctgcatcgag cggtatgggg ggagggtttt tgctgacacc tgtatggttg
     1561 tatcccccgc tcttgagcaa tatggggcaa tcatggtaaa ttccggaaaa gcattggcct
     1621 atgtccccaa tatgtgcggg gctgccgtcc ggatcggcac aacagaagag tgtatcaata
     1681 gtgcgacaga aggatcctaa cgggaccgga tccctatacg ggaccggggg aactgaacct
     1741 gattccagga actccttctg gctcgccatt ttccctcaat gagcaccttt aaatttcaat
     1801 ttatacaaca atcttcctga tatggccttt tcggacatca gaatggaaag cttcagaaat
     1861 taccggatgc ccgtcctcgt gctccttgtt gtgatttcca tccttcttga gatagtgctg
     1921 cactggtatc tgggaatatc tgttgcattt tctcattttt tttatatccc ggttgtcctt
     1981 gccgcagttt ggtacggcaa gcgggcggtt gttgtcgcaa tgattttagg aattgcccat
     2041 cttgccggga cctatttttc gattggattc attgataccg tggctctcct cagggcgatg
     2101 atgtttatcg tcatagcgtt ggtactgggc gcgataaccg atcgtatgaa aaaagagcag
     2161 gagcagatta tcaataaagt gaccgatgcc gcattcagtg ccgggctcaa tgaaggcagc
     2221 accagaggta ttcagggccg gaaaagccgg atcccctctt ttgccagcgt gaaaacaatg
     2281 agtgaacgcc gtgatatccc tggtcttatc agggcgctca aaaaccggga tcaggcagtc
     2341 cagtatcagg ctgcggaagc cctagggaat attggtgatg cttcggcaac cgaagcgctg
     2401 atggatgcac tgaccggaga tcaatacagc gggattcggt ggaaagcggc cgaggcactt
     2461 gggaagattg gtgcacccgc agtcccacat ctgatacagg ccctgaaaaa tcctgatgag
     2521 gatgtcaggt ggaaagcggc tgtaacactc ggggaaattg gagatctgca atctgctgag
     2581 ccactcgtgg atctgctagg tgacgaggat cggtttgtca ggagccgggc tgcttatgcg
     2641 ctcggactgc tcggttctag cgcagtggca cctctcacca aggccctcct gaatggctct
     2701 attgagatcc gccggggggc tgctgcagcc cttggaacca tccgggatcc aaacgcagtt
     2761 gaggcactga tccaggcgct cggcgatcct gtggatgagg tatggcagga tgttatagga
     2821 gctctttcgc ggttggggga gttgtcgtat catccgctgg tcaatgccct gcgttctcat
     2881 gaaaggagga gtcttgaggg agctgccctg gctcttgccg gatcaggtgc acccgaggcc
     2941 gtcggcacac taacttctgc cattgacacc gcagatcctg caatacggcc ggttctccag
     3001 tcgattgtca atgaggtgat ggcccggcag aaccggcagt cttcgaggaa tggggaagag
     3061 ctaccaggat tttcaacaaa agaaccatca gggtcctctt gactgattat tgttcacgct
     3121 catcaggaat tgccggtgtg gaggatatgc gtatgaaaat ggaaatttcc agggatatta
     3181 aggcacttgc cgatccggat cgaaacatcc gcatcaaagc agtcaaacgc ctttcagata
     3241 tcgggcagcc tgctgttgaa ccgttgatca gggcaatgga gcatgaggag agtaaggatt
     3301 tccgatggta tgctgctgtt gcccttgccc ggatcggtca gccggcaatt gagccgctga
     3361 tcaccgcgct gaaaggaaac caggacaggg aatttcgtcg ctatgcttct gcagccctcg
     3421 gtcatatggg tggggaagct gtcgatcccc ttcttttggc gatgaagagc gccgatccct
     3481 caatgagggg ttttctttcg gcagcactct gccgcatagg ccccgctgcc attgaacggc
     3541 tgaaggaagc atatgccagc cacgatccgg atctcagaga atgcgcttcg cttacgctct
     3601 ggcggatggg tgaatccggg gtaatcggga tgatgcaggc gattgaaaga gagaacgagg
     3661 gaccgtgaac cggatcgatc cactttttgc gttccggata cgaaacagaa actaaggagt
     3721 ttgaaatggc gctgcagcga tccggggtga tggaatcgta tgaaaagagc cttcaggacg
     3781 gattgggagc agccctagag gtagcgaccc gagcccgggc agtggggatc gatccccgga
     3841 cgagagttga gattcctatc gccagtgacc ttgcagaccg ggttgaggca ctccttggaa
     3901 tccgcgggat cgcagcccgc atcagggagc tggaatccgg aatgagccgg gaggaggctg
     3961 cactcaggat cggtgacgat tttgttgagc gccggtttgg cgaacaatcc cgcgaagaga
     4021 tccttgacca tgccatccgg acggccatgg ccgtgctcac cgagggggtg gtggcagccc
     4081 cgaccgaggg gatcgcaaag attggaattg gaaagaatga tgacgggacg gaatacctcc
     4141 gaatttatta tgctggtccg atacggagtg cagggggcac agcccaggca ctctctgttc
     4201 ttgtgggaga ttatgtcagg cgggcactcg gcatcaaccg gtatattccc cgtgcggaag
     4261 aggtcgaacg gtatatcgag gagatccggc agtataacac gatcatgaac ctccagtacc
     4321 tccccggaga gagagagatc cgcctcatcg ttggtaattg ccctgtatgc attgatgggg
     4381 aaggaacaga gcaggaagag gttggaggat accgcaatct ggaacggatc gagacgaatg
     4441 cggtccgggg ggggatggca cttgtgctgg ccgagggtct tgcgctcaaa gccccgaaag
     4501 tccagaagta tgtccgcgca atgaagatgg acgggtggga atggctcgat gagctgacat
     4561 cgggtgctgc gaagagctcg gatcccgatt ccgatacgat ccaaccaaag gacaagtatc
     4621 tccgtgatct tattggaggg cgccctgttt tctcataccc gatgaggaaa ggcgggttcc
     4681 gcctccgtta cgggagatcc aggaataccg ggttcgccgc agcaggaatc aatcctgcaa
     4741 caatgcatat cctgggtaat ttccttgctg tcggtaccca gatgaagata gaacggccgg
     4801 gaaaggccgc agggattgta ccagtcgaca ccatagacgg tccgatggtc aggttgaaaa
     4861 atggtgatgt gctccgtgtc gacgatgcag agacagcccg tgcccttgcc cctgacattg
     4921 acaggattct cgatgtggga gagatcctga tcagctacgg ggagtttatg gagaataatc
     4981 acctgcttgt accgtcagcc tattgtgagg aatggtggca gcttgaggga ggatttgagc
     5041 ggccttcgaa cgagcttgag gctatagccc tctgttgtga aggagcgttc ctgcacccgg
     5101 actatacctg gttctgggat gatatcagtc ccgggcaggt cgccgagctt gcaggattca
     5161 ttgaagagca cggggagatc cggcatgggg ttctccttct tccggatgag ccggccatta
     5221 aagagatact tgaggagctg ctcatcgccc acatggtcag ggaaaagatg atctgcatca
     5281 gcaccaacaa ggtgctcctt gcctgtcttg gccttgacct cactctcagg cgacgaaagg
     5341 aatgggaaaa tgcccccatg gaacgttcac ttgacctcgt gatgcatctc tcgggattta
     5401 aaatacgttc gaaagctgga acacggattg gtggcaggat gggtcgtccg gggaaatcga
     5461 aaccccgcaa aatgaatccc ccaccccatt ctctcttccc gcttggtgaa gcaggaggat
     5521 cccggaggtc atttcaggaa gcatctaacc atgcgcttga agcaaacaag gatgggggga
     5581 ttatccggat tgaggcaggg agaaggcgtt gcagtgtatg cgggaccgag acgtatcaca
     5641 accggtgcca atgtggcgcc cataccgaag cggtatacac ctgccaacga tgcaagagag
     5701 agagcttgga accagaatgc ccggtctgca aagttccctc atcctgttca catcatctcg
     5761 ccctcaatgt caaacaggag taccttctcg cgatggaacg gctggggagg cgggagaatc
     5821 ccgtcgggct tgtaaaagga gttaagggtc ttatctcccg ggaacgggca gttgaagaga
     5881 tcgagaaagg catactcagg gcagagaatg aactctttgt ctttaaggat ggaactatcc
     5941 ggtttgatat gatcgatctc cccctgactc atttccgtcc tgatgaagta ggggtgagcc
     6001 ctgaaaggct ctgtgagctt gggtacgcaa gtgatatcaa aggagtcccc cttacccgaa
     6061 ccgaccaggt gctggagctg aaaccccagg acatccttat ctccgaatcc tgtgctgatt
     6121 acatggtcag ggtcgcccgg ttcatcgacg aacttctcac ccggtgttac catcttccgg
     6181 cctactactc ggtcaattcg agaacagggc tgattggcca cctggtgatc ggcctggctc
     6241 cccataccag tgcaggggtg ctttcccgta tcattggtat ttcgagggca aatgtcgggt
     6301 atgcccatcc ttttttccac gcagcgaaac ggcggaactg cttttacggt gatacagaac
     6361 tggagttctt cgatggaaat gtatggagaa aacagcagtt gcgtcagttt gtgatcgaaa
     6421 atttcgatct ctcgaggcct ggcatagacc ggcttgggac gtactactct gacccgaggc
     6481 agggatacct gacacggacc attgatcgcg aaggaaagcc gcatctcagg aaagtaacgt
     6541 cggtatcaat tcataaaagt ccgcctcacc ttatcagttt tgagacaacc cagggacgga
     6601 ttattgcggt tactccagac catgcgatgc tggtatggga tctctgttat ctgcggaaga
     6661 tcagggcaga tgagctgaag gagggcgatc ctgtccctgt catggtaggg atgaacgtga
     6721 tcactgacca tatcaagcga cgcgatctcg tccccgctcc cgaagaccgg gtttattgct
     6781 tgaccgtgga agaggagcat acggtatgtg cgaatgggat attcaccggg caatgtgatg
     6841 gggacgagga ctgtatcatg ctccttctcg acggtctgat caatttctcc cgtgccttcc
     6901 tccctgagtc gaggggaggg accatggatg cacctctggt gctcactact tcgatcgatc
     6961 cagcagaggt tgacaaggag agccataacc tggatgtcat ggcttcttat ccccttgaac
     7021 tctacctggc aggccttcga tatgcccatc caagagagat cgtctctctg atcgaccggg
     7081 tcgaaaaccg gctgggaact ccagccgcaa tggaaggatt tctctatact cacgaaacat
     7141 cggatatttc agccggaccc cttgaatcca cgtataccca gttgaagacc atgattgaga
     7201 agctggaagc cgagctgaca ctcgcagaaa agattcgcgc ggtcgaccag gatgatgtgg
     7261 cggagcgtgt cctcactact cacttcatta gggacctgat ggggaatctc tctgcctttt
     7321 ccaaacagaa gctgcggtgt gtcaagtgca accacagtta ccggcgaatg cctcttgcgg
     7381 gcaagtgcat ccggtgcggg ggaaatgtta taccgacggt tcatgaagga tcggtaaaga
     7441 aatatctcga ggtatcacgt gctatctgcg agaaatacaa ggtatctgag tacacgaagc
     7501 agcgggtaat ggtcctcgat cgggcaattg agtccacatt tggccatgaa aaggaccagc
     7561 agctcggtct tgcagatttc atgtgaaggt tcggaacggt catcgtatgc gaccgggtat
     7621 tcaatttttc gggaaatcgg atcaattctc tggagtctga tgcattgatc atcccggaga
     7681 agcgaaatat ccggaaatcg caggaataac tcacgctttt attaccaatt ttggactata
     7741 tatgtagtga gcgagggtac ccaagctggc caacggcggc agactcaaga ccacataaga
     7801 attattgcgg tagatcaccc tctctataat ggtattttgg agcgagtgta accaagcggt
     7861 caacggtgac cgactcaaga tcggttctcg taggagttcg caggttcgaa tcctgccact
     7921 cgcatcgttt ttgctgtaac gtgaaatccc aatcatcttc agtcatcatt gccggtaaaa
     7981 ttcgagacgt attccttttt cataaccctc agtgaacgat tgcttattgt tttgatcatg
     8041 gctgccatgc atgcagaatc ccgaagagcc cgagaggtcc atgaaccggg tcccgatcga
     8101 tagaaaaagc atatttttct gaaatatact ggagaagttc cgtgttgatc ggccctccga
     8161 taggtggttg gtggagctcc atctcgattc tcctgattcc ctcgagatcg accgggcgta
     8221 tctgccattc tgcaccctca cagtcgcact tcagaaagtc acatcctcct gccctctgga
     8281 tcaggtcaga taatctatac gtggaatccc tggaagagat cccatcccat tcgatgtaag
     8341 tagggaaacc atctccaagc gccccgcaaa ataccttgat atcaaccccg ttcagggtga
     8401 tatttgtgtt gagaatgtcg gtggtgaatg gttcaactgc agagacatga gagcttttct
     8461 ttgcagcacg gatacagaat gcaccgatgt tggcgccaat atcgagaata tgatcatctt
     8521 cacggatatc ctccatccaa tattccggga tttctgtaat ctggcgatat ggtgtggcga
     8581 tgttactaaa cctgatacca tcacgggtag tgataaacga cgcgttgtgt cggaggtggg
     8641 gggaggatga tgcaggaaac cggctcattt tctccctcat ctcaggatag ctccattatc
     8701 cctatcagaa ataccagcat ccacaacttt catcatatcc cattatccac aatctctgga
     8761 atgaaccaga aagagttagg cctgcttttt gcggtggtag cactgcttat tggaggtata
     8821 attatttcgg cactatattt cttgccacct cccggatcca gcccggaagc tgaatattca
     8881 agtgaagtat caccgacttc accatccgct ccaaccgtag catcaccaac gatgtccgaa
     8941 tttgaaacaa cattccctcc cggatcggtc agtggatctg gaacgtaccc tgtcacttcg
     9001 aaacctacgg atgttttgtc taaccgctta tcaggatcag acgacgccgg gacctctttc
     9061 cccccaatcc agacaaccgc tgttacgaca tcccctgctg ccaccggact cccgtcattc
     9121 actttgattg tcacccctgt agaggcgcgg gcgaagcccg gggacaccat cctctatgtg
     9181 atggctatcg aaccgaaggg cgggtttgat gagccggtat ctctccgtct tgaggtaaat
     9241 gcactgcttc tgtaccggga gtcttttgat ctgggggata tcgatccacc ttttccaaag
     9301 acgatcgagt accggttcgt ggtaccttca gaagtaccgg gagggataac agtgaaaggg
     9361 cacatcaccg ctgagggtgg ag
//
LOCUS       DAOOCO010000058         9367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_6949_length_9367_cov_6.562070, whole genome shotgun sequence.
ACCESSION   DAOOCO010000058 DAOOCO010000000
VERSION     DAOOCO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6949_length_9367_cov_6.562070"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /gene="cgi121"
                     /locus_tag="PK154_04680"
     CDS             <1..350
                     /gene="cgi121"
                     /locus_tag="PK154_04680"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HPW10389.1"
                     /translation="FRAFERGEAISNTVEMEALLYAAGSRQCEVGMEFGVHPGVNRVY
                     LCICPGSDPVLRDLEQRGVYCNEDDWEQFSGEKQCLLQEIFGITDKELAVVGISRLQD
                     LVIERVALLEVYR"
     gene            complement(521..1591)
                     /locus_tag="PK154_04685"
     CDS             complement(521..1591)
                     /locus_tag="PK154_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW10390.1"
                     /translation="METTMHSPLIVEPHDPKWLLLQKVLAVVSTRRTKQELSKKGITP
                     VASAEQAIKIALVAMFFSVDIAFVVQEMRTRKKLRRFIGIPDNVSVDTIYRFFSKCDE
                     AQFVSVISGILNSCCTHRRKRGNMTFLIDATAITLDLNWFRRTFSKKQLEKREFKWGY
                     SPSHGHYIGYKLTLVIEYPSLHPVCILLHPGSPHDSVLYEEIMTDLKRRRIIRIGDLV
                     VFDKGYYSYGNYVNGIFKFNVVPLIFSKKNFSVKKLMNHLNYPIWIFGRSDTKLLMVR
                     FRSLASRLINHLRSISPFMEKRSLIEDVFKTAKNAFSLRKIHRYTSRSVAKTVCLNVL
                     LLGLVISLGFDKKVDLQRLSEW"
     gene            complement(1854..2342)
                     /locus_tag="PK154_04690"
     CDS             complement(1854..2342)
                     /locus_tag="PK154_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPW10391.1"
                     /translation="MNPEHVKPRTIRGAVITVSSSRTKETDKSGETLIRLLEQAKIPI
                     SHYAIVPDTIPAIRNEAIKAGAAAECIILSGGTGLTHDDCTIEAITPLLDKKIDGFGE
                     LFRLLSYQQIGSAAMLSRALAGIWGGKAIFCIPGSTGAVELATSKLILPEIAHILTHA
                     NK"
     gene            3400..4680
                     /locus_tag="PK154_04695"
     CDS             3400..4680
                     /locus_tag="PK154_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HPW10392.1"
                     /translation="MPDTPESVGLFKKYLSGNKIFKNREVLRHSYRPQILPHRRPQID
                     QVASILAPSLKNETPSNILIYGKTGTGKTAVVRYVGTELENASTHMGTSCRVVHLNCE
                     VIDTQYRVLAQISKSLSGDDEVSSDKVRSIIPMTGWPTDQVYQELKNQLESTGGVLVI
                     VLDEIDKLVKKSGDETLYNLTRINTDLKKSKVSMIGISNDLSFKDFLDPRVLSSLSEE
                     ELVFPPYNAPQLCDILFQRAEIAFLEGALDDGVIPLCAALAAQEHGDARRALDLLRVS
                     GEIADRDEAEGVTEKHVKIALAKIETDSMVECIATLPTQSKIVLYSMMLLDQMGQNIF
                     TSGEVSKIYKEIAPAMDLDILTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWFD
                     SNNHKIRDVILSDPRLSAHGLEKMDVTWVKTLFR"
     gene            4688..5179
                     /locus_tag="PK154_04700"
     CDS             4688..5179
                     /locus_tag="PK154_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW10393.1"
                     /translation="MDEKDLLLLRLLEENSRLSPDELAIMAELSGEDVRCRIAALEEA
                     KIIRSYTTIIDWERAANGGVAAIIELKVNPERDYGYDRIADRLAKFREVKSLRLVTGV
                     YDLQLHVTGKTMQDVARFVSEYIAPMDRIRETATHIIMKTYKENGQNFCEKEPGERLP
                     YSP"
     gene            5189..6352
                     /locus_tag="PK154_04705"
     CDS             5189..6352
                     /locus_tag="PK154_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW10394.1"
                     /translation="MRDFVSRRAKVIPPSGIRKFFDLALTMEEVISLGVGEPDFSTPW
                     NLCEASIYSIEQGGTSYTPNRGIQPLRDAISRWLSGHYGIDYNPDTEMVVTTGVSEGL
                     DIAIRAVTDPGDELIVAEPSYVSYAPCVTLAGGTPIPVECLEKDHFKLRADRLMEAVS
                     PKTKAVIINFPNNPTGAVMRQEDLKAVADVVIDHDLLLISDEVYSELTYTGTHASAAA
                     IEGLRERTILLNGFSKAYAMTGWRIGYLCAPKEICDAALKIHQYVMLCAPAMGQVAAL
                     EGLRRGEEEKNKMVQEYRVRRNLFVEGLNRIGLPCHLPEGAFYAFPSVEKTGMPDLEF
                     AECLLKEEHVAVVPGSVLGQGGKNHIRCAYAVSRADLAEAVKRMAHFIASNSC"
     gene            6600..7610
                     /locus_tag="PK154_04710"
     CDS             6600..7610
                     /locus_tag="PK154_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPW10395.1"
                     /translation="MSCTIIVGGFFGDEGKGKVVAHVAYEDHPTIISRGGVGPNAGHT
                     VTMGEKEYGVRMVPSGFVYPDARLFIGTGVLVDPRVLKHEVELLNVKDRTFVDYRCSV
                     IEQQHIERDRSSEHLAKTIGSTGSGCGPANADRVMRVARQAKDFPELAPFLIDVPAEI
                     NAALDMGESVLLEGTQGFGISLYYGTYPFVTSKDTSASQIAADNGVGPTRIDDVIVVF
                     KAYPTRVGEGPFSTEMTREVSDRLGIQEFGTVTHRQRRIGEWDGEMARYSAMINGCTQ
                     AAITGIDRVDPLCFGITDYDKLSEKALAFIEIAEEDIGAPVTLISTGPDVAHIIDIRD
                     EL"
     gene            7607..7789
                     /locus_tag="PK154_04715"
     CDS             7607..7789
                     /locus_tag="PK154_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HPW10396.1"
                     /translation="MRRSLLPILCCPVCKGDVVLTVAEEDEKEILEGDLHCPVCQVHY
                     PIEEGIPNLLPQSTVP"
     gene            7970..8923
                     /gene="cbiB"
                     /locus_tag="PK154_04720"
     CDS             7970..8923
                     /gene="cbiB"
                     /locus_tag="PK154_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HPW10397.1"
                     /translation="MEPDLIGHLIILNAIVLPLALLVDRLIGDPRSRYHPVALIGSFI
                     GWWGRPMLWQPWIQRIAGAGMWVVTVILFSLPFFLVSWLFPWFLFLPAGALLLKFCLA
                     WRSLEEHAAAVDLALGQNITEGQNTASLMVSRDTRDLTPEQVRSAAYESVAENLVDSI
                     VSPLFYFGLFGLPGAAVFRAANTMDAMLGYRDERKRIGWWPARMDDILNFIPARVAGG
                     LLLLYFAARGRFSQAWQGLIRDRRKRPGINGGIPMSIIAAGVGVRFEKTGVYVMGTGE
                     RSLEEAGEEIVKVVRVITVTFCALISMGLFLLGSGIIYTGL"
     gene            8920..>9367
                     /locus_tag="PK154_04725"
     CDS             8920..>9367
                     /locus_tag="PK154_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPW10398.1"
                     /translation="MKLEELRHGTELLKRGFASMQKGGVIMDVVNASQAAIAEQAGAV
                     AVMALERVPADIRKAGGVARMADPAIVLEIMDAVSIPVMAKVRIGHFVEAQVLESLGV
                     DMIDESEVLTPADEQFHIDKKQFSIPFVCGARDLGEALRRIQEGDGE"
BASE COUNT         2310 a         2254 c         2526 g         2277 t
ORIGIN      
        1 catttagggc atttgagagg ggtgaggcca tttccaacac agtggagatg gaagcacttc
       61 tctatgcagc gggatcgagg caatgtgaag tagggatgga atttggcgtg catccgggag
      121 tgaacagggt atacctctgc atctgcccgg gaagtgatcc agtactgaga gatcttgagc
      181 aaaggggtgt ttactgtaac gaggatgact gggaacagtt ctcaggtgaa aaacaatgcc
      241 tgcttcagga gatctttgga attaccgata aagagcttgc cgtggttggt atcagccggt
      301 tacaggacct ggtaatagaa cgggtggccc ttctggaagt atatcgttaa tctataaaaa
      361 tgcctcctcg ccattcggta gggagaggcg gaatcaagga tggccttcac ctaccaaaaa
      421 gtcaatgaac tgcacgtgag gcatccttca tgcaaaatgc atcgaatgtg gggtggagag
      481 gcaaagagcc cccataaaag gtattaaccc cctccccaga tcaccattca gagaggcgct
      541 gtaaatcgac ctttttatca aagcccaaag aaataacaag cccgagtaaa agtacattca
      601 aacaaaccgt ctttgccaca gatctcgaag tatacctatg gattttcctc aaggagaaag
      661 cattcttggc cgttttaaag acgtcttcta tcaaggatct cttttccatg aatggagaaa
      721 tactccgtag atgattgatc aaccgagatg ccaatgatct aaaccgaacc atgagtagct
      781 ttgtatcgga ccggccaaag atccagatcg gatagttgag atggttcatt aatttcttca
      841 cggagaaatt ctttttcgag aatatcaacg gtacaacgtt gaacttgaag attccattca
      901 cataattccc ataggaatag taacctttgt cgaagaccac taaatccccg attcggatga
      961 ttctccgtcg ttttagatcg gtcataattt cctcatagag gaccgaatca tgaggagatc
     1021 caggatggag gagtatgcag accggatgga gggagggata ctcgatgacc agggtcagtt
     1081 tataaccgat gtaatggcca tgactgggcg agtatcccca tttaaactct cttttctcga
     1141 gttgtttttt tgaaaaggtt cttctgaacc agttgagatc gagagtgata gctgttgcat
     1201 cgatcaggaa tgtcatattc cctcgttttc tccgatgtgt gcagcaggaa ttgaggatgc
     1261 cggatatgac ggatacgaat tgggcttcat cacatttact gaaaaatcga tagatagtgt
     1321 cgacggatac attatccgga attccaatga accggcgtaa cttcttccga gttctcattt
     1381 cctggaccac aaaggcaata tcgactgaga agaacatggc caccagggca attttgattg
     1441 cctgttccgc agaggccaca ggggtaatac cttttttcga gagttcctgt ttggttctgc
     1501 gggtggatac aaccgctaag accttctgca aaagcaacca ttttgggtca tgaggttcta
     1561 ctatcaaggg ggaatgcatt gttgtttcca aacttgtatt ccccttcgag tacgatcaat
     1621 tttgcggttt attcttactt gaattcttat ttggggggat taaaatctgt acttttattc
     1681 gatctatatg gattgtcctc caatcataaa ataagagaag atgcaaggct tgttagaaat
     1741 acgattcggg agggggttaa aaggtatctt gttcatcact cttacctaag caagatagca
     1801 aagagcctgg aacagacagg tacttctgtt cagatctctc taaaagtcga gaatcacttg
     1861 ttggcatgag tgagaatatg ggcgatttcc gggagtatga gtttactcgt tgccagttcg
     1921 accgctcccg tagagccagg gatgcagaat atcgcttttc caccccagat gccggccaga
     1981 gctcttgaaa gcatggcagc gctcccgatc tgctgatagc tgagcaggcg aaataattct
     2041 ccgaatccat caatcttttt gtcaaggagg ggggtgatag cttctatcgt gcagtcatca
     2101 tgggtcagcc cggtcccccc actcaatatg atgcactcag cagccgctcc tgctttgatt
     2161 gcctcgttcc gaattgcagg aatagtgtca ggaactatcg cgtagtggga gatagggatt
     2221 ttagcctgct caaggaggcg aatgagggtc tctccactct tgtcagtttc tttggtccgt
     2281 gagcttgaaa ccgtaattac ggccccccgg attgtccggg gtttcacgtg ttctggattc
     2341 atctggaaaa agattacaca caaaagataa tagagcaaca ggtatccata tgaggtagcc
     2401 tgaataccta atcgggcatg gtgaatcctg caaagagatg ctccaggaga tactctggat
     2461 gatgagcatt tccaagtgga agattcttct tgttatcatc tcacccggtc atcataagtg
     2521 aaagtgtggg gcatcacatt gagcagagat gaccaatgaa cagatataga aaattgattg
     2581 gttctgtacg gtcaaggata gcgatcaagc tgttatcaga atagtgagac ttttagactg
     2641 aaccgtcttt ttttccagat actggtcggt cgccccaccc ctttatttcc agtggagtta
     2701 gtaacgataa acgccgcctt tttctcctat tctttgatcc gggcagtctt ccattacgat
     2761 ccgcactaat ctggttttca gcaatgggtg aggagttctg atccgtacgg aatttcatac
     2821 cttcatgaca agagggtatc ggatgtgact aaagatttat agagatcggg aaatttcatt
     2881 ttaacacagt ctttttaaaa tattgtaatt gggatattct ggctgagttg gtagttttct
     2941 aaaattttaa ggttgaaaat ttattcccag atatcctgtt cattcgttgg gatatccgtt
     3001 gttctaatat aacaatacct ttccccttat gatattcagt gcatttttgt ttattttttt
     3061 gcaatgttcg ccagttcgag attaatttca aaattttttt ataatatgaa ggagttattt
     3121 accaaatgct ttcattctta ttttaagata cagtctatcc tgtatcatta attcaatctc
     3181 cagtggaaag catggggtgg ggggaccagt aagtatctga gcactcttat ggaaaacatg
     3241 gaatttcctc tctaaaataa attttcaatt cctttgaatt tttaaaaaat aaagaataat
     3301 ctcctttttt ggagtggaaa caaaggggtt tataagatct acaaatgagc aagtttttac
     3361 cataggatac ctaaccaatc actcccgggg gatccatcag tgccagacac acctgaatca
     3421 gttggtctat tcaaaaaata tctgtcagga aataaaatct ttaaaaacag ggaagtcctc
     3481 cgccattcct acaggccaca gatccttcca cacagaaggc ctcagatcga tcaggtagcc
     3541 tctattcttg caccttcact gaaaaatgag accccctcaa atatactcat ctacgggaaa
     3601 acagggaccg ggaagactgc ggttgtcagg tatgtaggga ccgagcttga gaatgccagc
     3661 acccacatgg gaaccagctg cagggtggtg catctgaatt gtgaagtcat tgacacccag
     3721 taccgcgtcc tggcacagat ctcgaagagt ctctctggtg atgatgaggt ctcaagcgac
     3781 aaggtccggt ccatcatccc catgaccggc tggccgaccg accaggtgta ccaggagctg
     3841 aaaaatcagc tcgaatcgac tggcggggtc ctggtgatcg tcctggatga gattgataaa
     3901 ctggtaaaaa agagtggtga tgagaccctc tacaacctca cccggatcaa taccgacctt
     3961 aaaaagtcca aagtgagtat gattgggatc tcaaatgatc tcagctttaa ggattttttg
     4021 gatccacggg tcctctcctc gctttccgaa gaggaactcg ttttcccccc ttataatgcc
     4081 ccccagctct gtgatatcct cttccagcgc gccgagattg cattcctgga aggggccctc
     4141 gatgatgggg tgattcccct ctgtgcagcc cttgccgcac aggaacatgg ggatgcccgc
     4201 agggcgcttg atctcctccg cgtttccggg gaaatcgctg accgagatga agcagaaggt
     4261 gtgactgaaa agcatgtcaa gatcgcactg gccaagatag agaccgacag catggtggag
     4321 tgtatcgcca cccttcccac gcagagcaaa atcgtccttt attccatgat gctccttgac
     4381 cagatgggac agaacatctt caccagcgga gaagtgagca aaatatacaa ggagattgct
     4441 ccggctatgg atctcgacat cctcacgcac cggcggatta ccgatctcat ctccgaactg
     4501 aacatgctgg gggtcatcaa tacgcgggtt gtcagtcgcg ggagatacgg gaggacaaaa
     4561 gagatgtggt ttgactcaaa taatcataag atacgggacg tcatcctgag cgatccacgt
     4621 ctgagtgccc atgggcttga aaagatggac gtgacctggg tgaagaccct gttcaggtga
     4681 aacgaagatg gatgaaaaag acctcctcct cctgcgactt ctcgaggaga acagcaggct
     4741 ctcccctgat gagctggcga tcatggccga actctccggg gaagatgtca gatgccgcat
     4801 tgctgcactg gaagaagcga agatcatcag aagctatacc actatcatcg actgggaacg
     4861 ggcagcaaac gggggagtcg cagccatcat cgagctcaag gtgaatcctg aacgcgatta
     4921 tggctatgac cgcattgccg atcggcttgc aaagttccgg gaagtcaagt cactccgcct
     4981 cgttaccggg gtctatgatc ttcagctgca tgtgaccggg aaaaccatgc aggatgtggc
     5041 acggttcgta tccgagtata ttgcaccgat ggaccggatc cgcgagaccg ccacccatat
     5101 catcatgaag acctacaagg agaacggcca gaatttctgc gaaaaagaac caggggaacg
     5161 cctgccctac tcaccgtgag ggaacaccat gcgggacttt gtatcccggc gggccaaggt
     5221 gataccgcca tccgggatcc ggaaattctt cgacctggcg ctgaccatgg aagaggtgat
     5281 ctccctcgga gtaggggaac ctgatttcag caccccctgg aacctctgcg aagcaagcat
     5341 ctattcgatc gaacaagggg ggacctccta tactccgaac cgcggtatcc agccgctgag
     5401 ggacgcgatc tcccggtggc tctccggtca ttatggcatc gattacaacc ccgataccga
     5461 aatggtcgtc accaccggcg tatcagaagg tcttgacatc gccatacggg cggtgactga
     5521 cccgggggat gagctgatcg ttgccgaacc gagttatgtc tcatacgccc cttgcgtgac
     5581 gcttgccggc gggacaccca tcccggttga gtgcctggaa aaagatcact tcaaactcag
     5641 ggcagaccgc ctgatggaag cagtctcccc gaagacaaag gcggtgatca tcaacttccc
     5701 gaacaacccg accggcgctg tcatgaggca ggaagatctc aaggctgtcg cggatgtggt
     5761 aatcgaccat gacctcctcc tcataagcga cgaggtctat tcagagttga cctataccgg
     5821 tactcacgca tcggctgctg ctattgaagg gttgcgggag cggacgattc tcctcaacgg
     5881 attttcgaaa gcgtatgcca tgacagggtg gaggatcggg tacctctgcg ccccgaagga
     5941 gatctgcgat gcggcactca agatccacca gtatgtcatg ctctgtgcac ctgccatggg
     6001 gcaggtggcc gcactggagg gtctccgccg tggtgaagag gagaagaaca agatggtcca
     6061 ggagtaccgg gtacggagaa atctgtttgt ggaggggctg aaccggatcg ggcttccctg
     6121 ccatcttccc gaaggagcat tctatgcttt cccgtccgtg gaaaagacag gaatgccgga
     6181 cctggaattc gccgaatgcc tgcttaagga agagcatgta gcagttgttc cggggagtgt
     6241 tctcgggcag ggtgggaaaa atcatatcag atgcgcctac gcggtctccc gcgcagatct
     6301 tgcggaggcg gtcaaacgga tggctcattt tattgcttcc aactcgtgct gacagcaccc
     6361 tttcctctct tttatggaat gtaccctctt gccgggatga atggataatc gtccacctgg
     6421 cgatcaagag tggattcatt ttccagggct ctctgctccc tgtttcagag agagatggtt
     6481 tcgagcagtc tcctctttcc ggagaggggg aatccgccga atcgtgacag gatcctcgcg
     6541 attggaagag aagtaactag ttttatggca ccggcctccc tatactagat ggttggacaa
     6601 tgagttgcac aattatcgtc gggggatttt tcggtgacga ggggaaggga aaggtcgtgg
     6661 cccatgtcgc ctacgaggat catcccacca tcatctccag ggggggtgta gggccgaacg
     6721 caggtcatac cgtcacgatg ggggagaagg agtatggagt gcggatggtg ccctccggct
     6781 ttgtgtatcc ggatgcccgg ctgtttatcg ggaccggggt acttgttgat ccgcgggtcc
     6841 tcaagcacga ggtagaactc ctgaacgtta aggatcggac gtttgttgat taccggtgca
     6901 gtgttatcga gcagcagcac atcgagcggg accgctcaag cgaacacctg gcgaagacga
     6961 tcgggagcac cgggagcggt tgcggcccgg caaatgctga ccgggtgatg cgggtggctc
     7021 gccaggcaaa ggatttccct gagctcgcac cattcctcat cgatgtccct gcagagatca
     7081 atgccgccct tgacatggga gagagtgttc tccttgaggg gacccagggg ttcggcatct
     7141 cgctctatta cgggacctat ccttttgtca cgagcaagga tacctctgcc tcacagattg
     7201 cagcagacaa tggggtcggg cctacccgga tcgatgatgt aatcgtcgta ttcaaggcat
     7261 acccaacgcg ggttggtgaa ggtcccttct cgaccgagat gacccgtgag gtatccgatc
     7321 ggcttggcat ccaggaattc ggtaccgtga cccaccgaca gcgcagaatc ggggaatggg
     7381 acggtgagat ggcccggtat tctgccatga tcaacgggtg tacccaggcg gccattaccg
     7441 gaatcgacag ggtggacccg ctctgttttg gcatcacgga ttacgacaag ctcagtgaaa
     7501 aagcccttgc atttatcgaa atagccgaag aggatatcgg ggctcctgtt accctgatct
     7561 ccaccgggcc ggatgttgcc catatcatcg atatcaggga tgaactatga ggcggagcct
     7621 gcttcccatc ctctgctgtc ccgtctgtaa gggtgacgtg gtgctcacgg ttgctgaaga
     7681 ggatgagaag gagattctcg agggggacct ccattgtccg gtatgccagg ttcattaccc
     7741 gatcgaggag ggaatcccga acctccttcc acagagcacg gtcccataac acactcaggt
     7801 atcccccata ttttggctct gaaataagcc gatggattat aatgaggagc caggcgaggg
     7861 gatgctccat tcaatctttg attctgccag gaatttcaaa gggacgtgga ttatgaggga
     7921 aaacagcgat actcaatagg cttaccaaag aacatcgtgg ccgcatacta tggaaccaga
     7981 tcttatcgga cacctgatca tcctgaatgc aattgttctc ccgctcgccc tcctcgtgga
     8041 ccggcttatc ggggacccca ggagccggta tcacccggta gcccttattg gatcatttat
     8101 aggatggtgg ggacgcccga tgctctggca accctggatc caacgaattg cgggcgctgg
     8161 aatgtgggta gtgacggtga tccttttctc gctcccgttc ttccttgttt cctggctgtt
     8221 tccctggttt ctcttcctcc cggccggggc actcctcttg aaattctgcc ttgcctggcg
     8281 atcacttgaa gagcatgctg ctgcggtcga cctggcacta ggccagaata tcacggaagg
     8341 gcagaataca gcttccctga tggtgagccg tgacacccgg gacctcaccc cggaacaggt
     8401 acggtcggct gcgtacgaat cggtggctga gaacctcgtc gacagcatcg tctctcccct
     8461 gttctatttc ggactcttcg ggctccccgg ggcagcggta ttccgggcgg caaataccat
     8521 ggatgccatg ctcggctatc gggacgaacg gaaacgtatc ggctggtggc ctgcccggat
     8581 ggatgacatc ctgaacttca tcccggcccg ggtagccggg gggctgcttc tcctgtactt
     8641 cgccgcccgg gggaggttct cccaggcctg gcaggggctc atcagggacc ggagaaaaag
     8701 gccggggatc aacgggggta tcccgatgtc aatcatcgcc gctggtgtcg gggtccgttt
     8761 cgagaaaaca ggggtctatg tcatgggaac cggggagcgg tcccttgaag aggcaggaga
     8821 ggagattgtg aaagtggtcc gggtgattac cgttaccttt tgtgccctta tcagtatggg
     8881 actattttta ttgggatcag gaataatata tacaggatta tgaaactcga ggagctgaga
     8941 cacggcaccg agctcctgaa gcgcggcttt gcgtccatgc agaaaggggg agtgattatg
     9001 gatgtggtca atgccagcca ggcagcgatc gcggagcagg ctggggcggt agcggtcatg
     9061 gcgcttgagc gtgtccctgc agacatcagg aaagccggcg gtgtcgcccg gatggcagat
     9121 ccggcgatcg tccttgagat catggatgcc gtatctattc ccgtcatggc gaaagtgaga
     9181 attggtcatt ttgttgaagc gcaggtcctt gagtcgctcg gtgtggatat gattgatgag
     9241 agcgaagttc tgacccccgc tgatgaacag ttccatatcg acaagaagca gttctccatc
     9301 ccgttcgtct gcggtgcgcg ggaccttggt gaggcattac gccggatcca ggaaggggat
     9361 ggagaac
//
LOCUS       DAOOCO010000059         9202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7116_length_9202_cov_5.904996, whole genome shotgun sequence.
ACCESSION   DAOOCO010000059 DAOOCO010000000
VERSION     DAOOCO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7116_length_9202_cov_5.904996"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK154_04730"
     CDS             <1..427
                     /locus_tag="PK154_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10399.1"
                     /translation="QEPKDWKNTDRNVLSDVYYVLTDKPNLHHVFPINYVNKNPGKNK
                     VNANSLMNIAYLTQITNIEISDQNPLEYVKQFDNKAFESIMKAHFLPSELLDWSRSDR
                     MPENALDHFIEERMELILQSIKNNLPDLNVEVFDSSTTL"
     gene            complement(775..1479)
                     /locus_tag="PK154_04735"
     CDS             complement(775..1479)
                     /locus_tag="PK154_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPW10400.1"
                     /translation="MVLPDLLSLSDIILNIQEQIPQMMEIYGIWIYAILFAIIFCETG
                     LVFAPFLPGDSLLFLLGFLAAGEGLNIWVIIVILSIAAILGDSVNYRIGTSFGNWLVN
                     REKCLVDLKHIDMTRQYYHRYGKKTIILARFVPGIRSFAPFVAGIVKMKYPVFLVYNV
                     VGGMLWVTGFVLFGYIVASLPIFKEHQDWFLWAILIISIGIFFMMMYNFRKSMKECVR
                     TPEEMLKEMASDIELK"
     gene            complement(1600..4167)
                     /locus_tag="PK154_04740"
     CDS             complement(1600..4167)
                     /locus_tag="PK154_04740"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPW10401.1"
                     /translation="MIPFRDLPESARTHGLSSESVLTMREQHGRNELTPPRRTPLWIQ
                     FLSKFNDPIIRILLVAVFISAIVAVVEGGSLIDTIGIVMAVLLATGISFFTEYRSNRE
                     FEALNAMREDTGIKVIRDDRAATVPMRDLVVGDLILLEAGDVVPADGYLLQASNFEVD
                     ESAFTGESEPLGKTVEEVVLKGTLATAGRGRMIIGAVGDATRMGQIAADLAEGSRPET
                     PLQLKLRRLAGLVSKFAYLMAGLIIATLMIEGIFTGVFTESPAAIAHHILDSFMFAVI
                     IIVVSVPEGLPVSVTVSLALTMRKMTRAFSLVRRLIACETVGSVTVICTDKTGTLTMN
                     QMDVVASSVEFPDLQTGIPTTPNGWVTLNAAVNSTAYLESREGRLVTVGNSTEAALLR
                     WLHRAGLSYQEIRALHPPVTQELFDSNKKQMSTIISLQNTAFVLVKGAPEIVAKQCNP
                     PPDLSHIQALAGRAMRTLAFAHAILPADGSTHPPLTWDGYVGIRDEVRPDVPDAVRTC
                     NQAGITVKMVTGDSPETAAAIARETGILTSGSVMLGSEFRSLPETARPDAARRLEVMA
                     RSEPSDKLLLVQALQGNGEVVAVTGDGTNDAPALRNADVGLAMGIAGTEVAREASDII
                     LLDDSFPTIKNAVWWGRALYENIQRFLIFQLTINISAAMLSFLAPVLGYPPPFTIIQL
                     LWINIVMDSLAALALCSEAPHPALMHRKPIPRDAPVITPYMLRAILITAAVYVLVGML
                     VVVFGLPFMETPEQQATAFFAAFVVAQVWNGINCRGINGIMPPFFKGNPAFFGIMAGI
                     VVIQILIVQYGGALFATVPLTAARWGFIILCTAPVLLLWPIIRSSTTLLRIPGRSGS"
     gene            4275..4601
                     /locus_tag="PK154_04745"
     CDS             4275..4601
                     /locus_tag="PK154_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPW10402.1"
                     /translation="MSYLIEIAIILILIVLNGIFALSEFAIVSAKRTRLQQRADEGDT
                     GAATALELAAEPTPFLSTIQFGITLVGIFAGAYGGATVAEGLAEYFTTFPSEPVGQSD
                     GNVYSN"
     gene            complement(4784..5302)
                     /locus_tag="PK154_04750"
     CDS             complement(4784..5302)
                     /locus_tag="PK154_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HPW10403.1"
                     /translation="MTETIRYEESGGAGEVEFRRYPSIILATVTGTNENENFWILFRY
                     ITGNNRSGKKVPMTSPVITPEKIAMTTPVISETNAMSFVLPAAYRMKDVPGPIDTRIR
                     IEEIPPRELAVIRFSGKASAIDVAQIRERLLKKLEESNITVVGTPFLMRYNAPYTPGF
                     MRRNEVGVEIKR"
     gene            5698..6174
                     /locus_tag="PK154_04755"
     CDS             5698..6174
                     /locus_tag="PK154_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPW10404.1"
                     /translation="MTHVPMVKPGDPAKNFSLKDQNDKTFGLYEQSGKHVLLSFHPLA
                     WTDFCAAQMTSLEDNRELLASINCVPVGISVDSRPCKRAWAASLSIKHTPLLCDFWPH
                     GAVAQKYGLFRDENGFSERANVVVDKNQLVVFVKVYPIHSIPDIQEVIVFLQKLAA"
     gene            complement(6325..7875)
                     /locus_tag="PK154_04760"
     CDS             complement(6325..7875)
                     /locus_tag="PK154_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HPW10405.1"
                     /translation="MRASARKKRADFTAGHEATVVYPHQLFQESPALANDRPVVLAED
                     PWFFKRFRFHAQKLVLHRASMRRYRDHLAAGGFRIEYIEAADLETSMDLIVQQARKGI
                     SEIHLCDPLETKLSEEIEKACSMQGIRLVTYESPMFLCSRDYLAEFYKGGRRFYLTEF
                     YIAQRKRHNILLEQEGKPVGNRWTFDTLNRRLLPKNFPVPPVWHPAENEYVPEAISYV
                     KDHFPYACGNVTPFFWPVTFEDANRWFEDFLVNRLPFFGRYEDAIHRNEPVLFHSALS
                     PLLNIGLLTPGNVVAATLAYAKDHEVPIESLEGFIRQVIGWREFIRAVYLLAGKKEQS
                     SNVLEQTQPIPSCFWDASTGIEPVDTTIRRVLATGYCHHIERLMVLGNIMILAGFHPG
                     RVYDWFSELFSDAYDWVMVPNVYGMSQFADGGMMSSKPYISGSRYILKMSDYPKGPWC
                     DLWDALYWRFLYVHRDLFEHNPRMAPFMTYITRMPEEKRQQLIEKAERYLDSIGACTG
                     MPPHSLEK"
     gene            complement(7879..8907)
                     /locus_tag="PK154_04765"
     CDS             complement(7879..8907)
                     /locus_tag="PK154_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPW10406.1"
                     /translation="MVRKFSRPRLVVSRCIEFDPCRYDGSKIPSPVVDHLKAHADFIP
                     VCPEIEIGLGIPRATVRIVRIEGVDHLIQPATGRDVTSDMTSFAGQFLANLPPIDGFI
                     LKGGSPTSGINNVKVYPSAEKSMAIERTAGFFAREVLKRFSSLPIEDELRLMNARIRD
                     HFLAGIFTHAAFREVEQTMDREALARFHAANKLILMACHQQNLRKMGQLIAMRSKKEP
                     EALFVQYHELLCISLKNVPRYTNNINVLQHVLGHFSNRVTSEEKGYCLRLIDRYRNGY
                     ATLAEPRDLLRSWVIRFNESSLMDQTFFAPYPFELADLPADMTERGRDVWKSLKSSNT
                     HKTERESR"
BASE COUNT         2287 a         2381 c         2369 g         2165 t
ORIGIN      
        1 tcaggagcct aaagattgga agaatacgga tcggaatgtg ctttccgatg tatactatgt
       61 tctcactgac aagccaaatc tacaccatgt ttttccaatc aattatgtca ataagaatcc
      121 tggaaaaaac aaagtcaatg cgaatagtct gatgaatatt gcatacctta ctcaaatcac
      181 aaatattgaa attagcgatc aaaatccctt ggaatacgtc aagcaattcg ataataaagc
      241 gtttgaatca atcatgaaag cacacttcct accgagtgaa cttcttgatt ggtcgcgatc
      301 ggatagaatg cctgaaaatg ctctcgatca cttcattgaa gagaggatgg agcttatcct
      361 acaaagtatc aaaaataact tacctgatct gaatgtcgag gtctttgatt cttcaaccac
      421 tctatgattt tttatcatgc cgcagggtca tttcactata aaaaccacct tgcacttttt
      481 cacccgcatt tctgcgcgag cataaaaata actgatttac gggctttgtt gacaacccgg
      541 ctggggcttt gaacatagct taaattgaga tcaagattgc cttctatttt gaaaatattt
      601 tggaaaataa ggctaaattt tgagattatt ttacaaacat cctgaaagaa cagggacctc
      661 agcggaagga atgaagcggg gatgaccatt ccgggccgtc actgtctcag catggtccag
      721 atttacgtca tgaagtaact cctcgtaaaa cttccacacg aaatgatacg gttcttattt
      781 caactcgata tcactcgcca tttccttaag catctcttca ggggtcctga cgcattcctt
      841 catcgatttc ctgaaattgt acatcatcat gaagaagatc ccgatgctga tgataaggat
      901 agcccacagg aaccagtcct gatgttcctt gaagattggg agcgatgcaa caatgtaccc
      961 aaagaggacg aaaccggtga cccagagcat ccccccgacg acattgtaaa cgagaaacac
     1021 cgggtatttc atcttcacaa ttccagccac gaagggtgca aatgatcgga tccctgggac
     1081 gaaccgggcg aggataattg tcttctttcc gtagcgatgg taatattgcc gggtcatatc
     1141 aatgtgcttc aggtcaacca ggcacttctc ccgattgacc agccagtttc caaaggaggt
     1201 cccgatccgg tagttgaccg aatccccaag aatagcggcg atggagagaa tcacaatgat
     1261 tacccagata ttcagacctt cccctgcggc gagaaagcca agaaggaaga gcagggagtc
     1321 acccgggaga aatggggcaa atacaagccc agtctcgcag aagataatcg caaacagaat
     1381 agcgtaaatc cagatcccat agatctccat catctgggga atctgctctt gaatattcag
     1441 tatgatgtcg gacagtgaca ggaggtccgg cagaaccatg tcaaatattg ggcagtacgt
     1501 gataaataga ctggcataat gatcgcccgc aagccccggg aaaggtttgc ttctaaaaca
     1561 acaaaaatgg actgggggcc agtcgccact tcgggctgat tatgaacccg atctccctgg
     1621 aatccgcagc aatgtagtac tcgacctgat gatcggccaa aggaggagga ccggagcggt
     1681 gcacaggatg atgaaccccc accgggctgc cgtaagcggg actgttgcaa agagagcacc
     1741 gccgtactgg acgatcagga tctggatcac cacgatccct gccatgatcc caaaaaatgc
     1801 aggattccct ttgaagaacg gtggcatgat gccattgata ccgcggcagt tgattccgtt
     1861 ccagacctgt gccacgacaa aggctgcgaa gaaagccgtt gcctgctgtt ccggggtctc
     1921 catgaacggg aggccgaaca ccacgaccag catcccaacc aggacataga ccgctgcggt
     1981 aatgaggatc gccctgagca tatagggagt aatcacgggg gcgtcccgcg ggatgggttt
     2041 ccggtgcatc agggctggat gcggggcctc cgaacacaag gcaagcgctg cgagcgaatc
     2101 catgacaata ttgatccaga gcagctggat aatagtgaac gggggagggt aacccaggac
     2161 cggggcaagg aacgagagca tcgcggctga gatgttgatg gtcagttgga agatgaggaa
     2221 ccgctggatg ttctcgtaga gcgccctccc ccaccacact gcgttcttga tggtcgggaa
     2281 ggaatcatcg agaaggatga tgtcgctcgc ctcccgtgca acttcggtgc cggcaattcc
     2341 catggcaagc ccgacatcgg cattgcggag cgcaggggca tcattcgtcc cgtcaccggt
     2401 gactgcgacc acttcgccgt tcccctggag ggcctggacc aggagcagct tatcggatgg
     2461 ctccgatcgt gccatgacct ccaggcgccg ggcggcatcg ggtcgtgctg tttcaggcag
     2521 ggaccggaac tcgctcccga gcatgaccga accgctggta aggatccccg tctcccgtgc
     2581 gattgccgca gcggtctcgg ggctgtcccc tgtcaccatc tttaccgtga tgccggcctg
     2641 gttgcaggtc cggaccgcat cagggacatc cggccgcact tcgtcgcgaa tacccacata
     2701 cccgtcccag gtcaagggag gatgagtgga tccatcggcg ggcagtatgg cgtgcgcaaa
     2761 cgcaagggtg cgcatcgccc tgcctgcgag cgcctggata tgagagaggt ctggcggggg
     2821 attgcactgc tttgccacaa tctcgggtgc acctttgacc aggacgaatg ctgtgttctg
     2881 gagggagatt atcgtcgaca tctgcttctt gtttgaatca aaaagctcct gggtgacagg
     2941 ggggtgaagg gccctgatct cctggtagga gaggccagcc cggtgaagcc agcggagcag
     3001 ggcagcctcg gttgaattgc ccaccgtcac aagcctaccc tcccgggact cgagataggc
     3061 ggtactgttc actgctgcat tgagggtgac ccacccattt ggtgtggtgg ggatgccggt
     3121 ctggagatcc ggaaattcaa ccgatgacgc cacgacgtcc atctggttca tggtcagggt
     3181 gccggtctta tccgtgcaga tgaccgtcac cgaaccgact gtttcacacg cgatcaaacg
     3241 acgcacgagg ctgaacgccc gagtcatctt ccgcatcgtg agtgccaggg agaccgtgac
     3301 actgacaggg agaccctcgg ggaccgagac gacgataatg attaccgcga acatgaagct
     3361 atccaggata tggtgagcga ttgcagccgg actctcggtg aagacaccgg taaagatccc
     3421 ctcgatcatc agagttgcga tgatcagccc cgccatcagg tacgcgaact tgctgaccag
     3481 tcccgcgagc cgccggagtt tgagctgcag cggggtctcc gggcgggatc cctcggcgag
     3541 atccgcggcg atctggccca ttcgggtggc atcccctact gcgcctatga tcattctccc
     3601 ccggccggcg gtggccagag tccctttcag gacaacttcc tcgactgtct ttccgagtgg
     3661 ctcgctctct cccgtgaatg cggattcgtc cacctcgaaa ttcgatgcct ggaggagata
     3721 cccatctgcc ggaaccacat cacctgcttc aaggaggatt aggtcgccca ctaccaggtc
     3781 ccgcatggga accgtggccg cacggtcgtc acggatcact ttgatacccg tgtcttcccg
     3841 catcgcgttc agggcttcga attcccggtt gctccggtac tcggtgaaaa acgagatgcc
     3901 ggtcgcgagc aggacggcca tcacgatccc gatggtatcg atgaggcttc ccccctccac
     3961 gactgcgacg atagccgata tgaagaccgc gacgagtaaa atccggatga tcgggtcgtt
     4021 gaactttgag aggaattgta tccacagggg tgttcgtctc gggggagtca gctcgttcct
     4081 accgtgctgt tccctcatgg tcagaacaga ttcagaagag agcccatggg tgcgggcaga
     4141 ttctgggagg tcccgaaagg ggatcatttc agcagatatt atccctgaat gtttttcatc
     4201 ctttgggcgg aaccccaggg aagagaaccg gcgaggaaat agctataatg tatcatcggg
     4261 aaagtattct cgaaatgtcc tatctgatcg aaattgcgat catccttatc ctcatcgtcc
     4321 tcaacggtat tttcgcactg tcggagtttg ctatcgtatc agcaaagcga accagactcc
     4381 agcagcgggc cgatgaggga gataccggtg cagccacagc actcgaactc gcagctgagc
     4441 ctaccccgtt tctctcgacc atccagttcg ggatcaccct ggtagggatc tttgccggtg
     4501 catacggggg tgcgaccgtt gcagaaggac tggcggaata cttcaccaca ttcccctcgg
     4561 aaccggttgg acaaagtgat ggtaatgtgt attccaactg atcccccaat atgactatta
     4621 acaagatcct gccttatacc acactttcga ttccttgtaa aaccacaata cacgtttcta
     4681 ctcgtatttc tgctcgagca caaaaaaatg atacaagggc tccgttgaca acccggctgg
     4741 ggcttttccg gcattgaatc tgggaccgtg aaatataccc gggctatcgc ttaatttcaa
     4801 cccccacttc attccgccgc ataaatcccg gagtataggg tgcattgtag cgcattaaaa
     4861 acggtgttcc tacgaccgtg atatttgact cttcgagttt tttgaggagc ctctcacgaa
     4921 tctgggcgac atcaatcgcc gaggcctttc cgctgaaacg gatgaccgcg agttcccttg
     4981 ggggtatctc ctcgatccga atccgggtat caattggtcc gggcacatcc ttcatcctgt
     5041 aagccgccgg caacacaaat gacatcgcgt tcgtctctga tatcacaggg gttgtcatgg
     5101 caatcttctc gggggttatg accggggagg tcatgggcac tttctttccg gaacggttat
     5161 tccccgtgat ataccggaag aggatccaga agttttcgtt ttcattcgtg ccagtaactg
     5221 tggcaaggat tatggaagga tacctccgga actccacttc tcctgcaccc ccactctctt
     5281 cgtaccggat tgtctcggtc atgattcctc catcacaggg ataattatct tagactatat
     5341 aattccgaac acggcaatct cctgggatca ggccggagag aatagagtat gcaatttcat
     5401 gaccccgatt tcatggaatt attctggttt ggttctttcg tttcaggaag actcataatc
     5461 ctgccaatgt tctgaattat tgtgaaatag tctgaccaac ccccgttcgt gaacctggtg
     5521 aaccatgtct tagcgggaga gtaatgataa ggatcaagca tcagcgaatc gtcgtgtgca
     5581 tctccgtgaa ttttgaggag accgtcctat caggttgagg agttggaaga tcctatatac
     5641 cctcatccaa aggttgaaag attctgcatc cctaaaacct gttgttttgg tgggtatatg
     5701 acacatgtgc caatggtaaa acccggcgat ccagcaaaga atttctcatt aaaagaccag
     5761 aatgacaaaa cattcggtct ctatgaacaa tcagggaaac acgtcctcct ctcgtttcat
     5821 ccgcttgcct ggacagattt ttgtgcagca cagatgacat cattggagga caaccgggaa
     5881 ctgcttgcct caatcaattg tgttccggtt ggaatcagtg ttgactcaag gccatgcaag
     5941 agggcatggg ctgcgagcct ctcgataaaa catacaccgt tgctctgtga tttctggccg
     6001 catggagctg ttgcacagaa atatggtctg ttccgcgatg agaacggttt ctctgagagg
     6061 gcaaatgtgg tcgtagataa aaatcagctc gtcgtctttg tgaaagtata ccctattcat
     6121 tcgataccgg atatccagga agtgatcgtg tttcttcaaa aactggctgc gtagaattcg
     6181 gcgggtctct ctcaaaatcg accattttta atttgatttt ctgtccgcgg cagacccgtt
     6241 accagccatc gaactctgtt atgaagaagg aggccttgtt aaaaaaattg cagttgtctg
     6301 cacaagaatg gattacaacc ttcttcactt ctctagggaa tggggaggca tgcctgtaca
     6361 cgcaccaata gaatccagat atcgttcagc cttttcgatc agctgttggc gtttctcttc
     6421 cggcatgcgg gtgatatagg tcatgaaggg agccatgcgt ggattatgct caaagagatc
     6481 tcgatggaca tagaggaagc gccagtagag tgcatcccag agatcacacc aggggccttt
     6541 cggatagtca ctcattttca gaatatacct tgatcctgaa atgtacggtt ttgaactcat
     6601 cattccgcca tctgcaaact gactcatccc gtagacattg ggcaccatca cccaatcata
     6661 ggcatcgcta aagagctcag agaaccagtc gtagactcgg ccaggatgga atccggcaag
     6721 gatcatgatg tttcccagca ccatgagccg ctcgatgtgg tgacaatatc ctgtcgcgag
     6781 gacacgtctg attgtggtat cgacgggttc gattcctgtc gatgcatccc agaagcagga
     6841 aggtattggc tgggtctgtt ccagaacatt ggaactctgc tccttttttc ctgccaggag
     6901 gtaaacagcc ctgataaatt ccctccagcc gataacctgg cggatgaacc cttcgaggga
     6961 ttcgatagga acctcgtgat cctttgcata cgcaagggtg gcagccacca catttccagg
     7021 ggtgagaagc ccgatattca gcaacggact gagcgcagaa tgaaaaagaa ctggctcgtt
     7081 ccgatgaatc gcatcttcat atcgtccgaa gaaaggcagc cggttcacga ggaaatcctc
     7141 gaaccagcgg ttcgcatcct cgaaggtcac cggccagaaa aaaggggtca cgttgccaca
     7201 ggcatacgga aaatgatctt tcacatagct gatcgcttca ggtacatact cgttctcagc
     7261 aggatgccag acaggaggta cggggaaatt ttttgggagg agtctgcggt tgagggtatc
     7321 aaaggtccag cgattcccga ccggttttcc ctcctgttcg agcaggatgt tatggcgttt
     7381 tctctgcgcg atatagaatt ccgtgaggta aaatctcctc cctcctttgt aaaactctgc
     7441 gagataatca cgggagcaga ggaacattgg actctcgtac gtgaccagcc ggattccctg
     7501 catcgaacag gccttttcaa tctcttcact cagcttggtt tccagcggat cgcagaggtg
     7561 gatttccgaa atgccttttc gggcctgctg gacgataagg tccatactgg tctccagatc
     7621 tgctgcttcg atatactcaa tcctgaatcc tcctgcagca aggtggtcgc ggtatctcct
     7681 cattgaggca cgatgtaaga cgagcttctg ggcatggaac cggaagcgtt tgaaaaacca
     7741 cggatcttca gcaaggacca ccggacggtc attcgccaat gcagggcttt cctgaaaaag
     7801 ctggtgggga tagacaactg tcgcttcatg gcccgctgtg aaatccgctc ttttttttcg
     7861 tgcagaggct ctcatttttt accgggattc tctctctgtt ttatgggtat ttgatgattt
     7921 cagggacttc cagacatccc gcccccgctc agtcatgtcg gcaggcaggt ctgcaagctc
     7981 aaatggatac ggcgcaaaga acgtttgatc catcaggctg gactcgttga accgtatcac
     8041 ccacgaacgg aggaggtccc ggggttcagc gagagtggca tagccgttcc tgtatcgatc
     8101 gatcaacctg agacaatacc ctttctcctc gctggtgaca cgattgctga aatgtccgag
     8161 aacatgctgg aggacgttga tattgttggt atagcgggga acgtttttga gtgatataca
     8221 gagcagctca tgatactgaa caaacagggc ttccggctct ttttttgacc gcatcgcgat
     8281 cagctgaccc attttgcgca ggttctgctg atgacatgcc ataaggataa gcttattggc
     8341 agcatgaaac cgtgcaaggg cttcccgatc catggtctgc tccacctcgc ggaatgcagc
     8401 atgcgtgaag atccccgcaa gaaaatggtc acgaatccgg gcattcatca ggcgcagttc
     8461 atcttcgatg ggaagggaag agaaccgctt tagtacttcc cgtgcaaaaa agcctgcggt
     8521 cctctcaatc gccatcgatt tttccgccga gggataaact ttcacattgt tgatcccgga
     8581 ggtgggtgag ccccctttca ggatgaaacc atcaatggga gggagattag cgaggaattg
     8641 acctgcgaaa ctggtcatat cactcgtcac atcccgaccg gtcgcgggct ggataaggtg
     8701 gtccactccc tcaatccgga cgatcctgac cgttgcccgc ggtatcccaa ggccaatctc
     8761 aatctcggga cataccggaa tgaagtcagc atgggctttc aggtggtcca ctaccggaga
     8821 aggtattttc gaaccatcat accggcacgg atcgaactcg atgcacctgc tgacaacaag
     8881 acggggtcgt gaaaattttc ttaccatgct atccctcact ccttctccat aaggacaatc
     8941 atgaagcctt tggctcccaa tcgccattat caccaatcct gaatataata aatgcaagga
     9001 atcgggatat tccgacacct agataccacg ataatccaga atacgcagaa tttatctcca
     9061 gataggatca tccccgaacc gttccatcca gaaatcagcg gggctctgct caaggtctcg
     9121 tgagaggtcc tcgatccggg acgagaaaac atggttcata caataatcag ctagaagatc
     9181 gtatgcccgc ttcaaccgga tg
//
LOCUS       DAOOCO010000060         9187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7138_length_9187_cov_5.485764, whole genome shotgun sequence.
ACCESSION   DAOOCO010000060 DAOOCO010000000
VERSION     DAOOCO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7138_length_9187_cov_5.485764"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PK154_04770"
     CDS             <1..1470
                     /locus_tag="PK154_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPW10407.1"
                     /translation="HLLSDRLRWLPLLTFLVVNPSFIWQVILGKTSGAVLWDSTRTIP
                     DTIHEYPAFSFVWGDLHAHVVSLFNQVFLLFLCVYALTHWKMMTPRHRFLLVILSALS
                     LGSMPLINTWDVLLYAPVTVLFGLLIWHENRESVGEGGSGTPPAIGPLERIMARAYSF
                     LLLVPPLSLLCYLPYYVQLKSGGIQGIGIVPTPSPVLAFLLVHGLFLILFLVYLRKDI
                     IRMPLLLLVPVPFILGGYAAAGIAALPLAYFLARRLRTPAEILAICGLSVIIFCEFFY
                     LKDNMGEVYYRMNTVFKFYLPAWILMASSGFAMLAVMLEQPVSHLKISKGLKRAALIG
                     VTALLLTAPLIIPFEYSSRDATLNGLAWLDTTHPGDAAAIAFLRSLSGSYGIVEAEGG
                     DYGYYSRISSSTGIPAIIGMPFHEYMWRADTWYGERVNDIRLIYEDPAQTVPLMRKYN
                     ATLLYIGDPERERYAVRAEEAGLPLLYNQSGVQIYLLSA"
     gene            1598..1966
                     /gene="rpl7ae"
                     /locus_tag="PK154_04775"
     CDS             1598..1966
                     /gene="rpl7ae"
                     /locus_tag="PK154_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HPW10408.1"
                     /translation="MAKTYVKFQVSEEIQNKALEALEIARDTGKIKKGANEATKAIER
                     SLATLVLIGADVEPEEIVMHLGPLCEEKKIPFVYINKQNDIGAASGLEVGSTAAAIVK
                     PGKAKDLIDEVAKQISDLRA"
     gene            1974..2183
                     /locus_tag="PK154_04780"
     CDS             1974..2183
                     /locus_tag="PK154_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HPW10409.1"
                     /translation="MADEGTPAEVIEVIGSTGMHGEAMQVKCRILDGPNKGRIITRNT
                     VGPIREGDVLMLLETEREAKKLSRR"
     gene            2191..2382
                     /locus_tag="PK154_04785"
     CDS             2191..2382
                     /locus_tag="PK154_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HPW10410.1"
                     /translation="MVEQKNCSFCGEPIEPGTGKMFVRKDSTIFYFCSSKCQNNFRLG
                     RTPRRVAWTKAGRKALGKE"
     gene            2389..2838
                     /gene="ndk"
                     /locus_tag="PK154_04790"
     CDS             2389..2838
                     /gene="ndk"
                     /locus_tag="PK154_04790"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPW10411.1"
                     /translation="MDRTFVMVKPDGVARGLIGEMVSRFEKKGLRLVAARLEKLPEKR
                     VMEQYREHLEKPFFPSLRHYITSGPVFLMVWEGKSAVTVVRALVGATNPAEAAPGTIR
                     GDFALDIGRNVIHASDSPESAKREIALHFTQEDFCSYKRVDEAVLYE"
     gene            3081..3728
                     /locus_tag="PK154_04795"
     CDS             3081..3728
                     /locus_tag="PK154_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HPW10412.1"
                     /translation="MAGEMVTFALLSLSSIIIIVNPLGATLTFVSLTSSLDRETRLKV
                     AVESCRYALAILIAFALAGGIILRLFGITIDAFRIGGGILLFVIGMEMVYARTSRSKL
                     TATEKYESMDAEDISVTPLAIPMIAGPGAITTTIVLMNEATGSVPRIALILVSIVIAI
                     LLTYLMMVRSDQIVSRIGQKEFRAINRLMGMLLIAIAVQFVIAGLTAAFPVLSGI"
     gene            3773..4564
                     /locus_tag="PK154_04800"
     CDS             3773..4564
                     /locus_tag="PK154_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPW10413.1"
                     /translation="MPIKIAGAQIGSRWEDPQQSLQKAEQSIRRAVNEGASLICFPEQ
                     YPTGWDPRSVRHVEEWDGPIVSRFRHLAQEYGIAILGSFRERSDPKPRNTCIVLDPHG
                     EIAATYAKSHLFTPAHEDEYYSPGTNPGIFRLSNVPFGIAICYDLRFPGLFGTYARAG
                     VCCMLVPAAWPASRIGHWELLLRARALEYQMYVMGVNPTGMTPVETYCGRSLAIDPSG
                     TVVAEAGGEEALIPAIIDPEVVHRVREALPVYQNGLLDDFSRNPA"
     gene            4631..5839
                     /gene="thrC"
                     /locus_tag="PK154_04805"
     CDS             4631..5839
                     /gene="thrC"
                     /locus_tag="PK154_04805"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW10414.1"
                     /translation="MYRLTCVQCGRSYPPDEIIYTCRSCGHLLFVEYDLDSLTIRREE
                     WDRRPISVWRYRELLPVTIPPVTLKEGGTPLYHLERIGEEIGLPHLYAKHEGMNPTGS
                     FKDRGMTVGVSMALQLNKSTVACASTGNTSASLAAYAAKAGIPAVVLLPAGKVALGKV
                     AQALMHGARVISIRGNFDKALEMVQELCISHGLYLLNSINPFRLEGQKTIGFEVIDQL
                     GSVPDRIVLPVGNAGNISAVHKGLGELHALGLIERYPMMTGIQAKGSSPVVRAIREGL
                     PEVIPEGEPETVATAIRIGAPVNAEKALTAIRKTGGTAEWVTDDEILAMQRDLARKEG
                     IGVEPASAASVAGVRKLALEGVIDPSERVVCVVTGHLLKDPETVIKQCKPPTEIDADV
                     QSLLSALRSS"
     gene            5773..6396
                     /locus_tag="PK154_04810"
     CDS             5773..6396
                     /locus_tag="PK154_04810"
                     /inference="COORDINATES: protein motif:HMM:NF039237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HPW10415.1"
                     /translation="MQATHRDRCGRAIAALCIAILLIPASVLALSAGYEILPNGTAYR
                     ASVEIVDADRYSFSETGFLGERIPVQVNDVSLSGDCPRNSCSYNSSDRFTITFPKGNY
                     TVHYTGSVRDNHFLATFPEPYLVQVILPPPFDVRNRFIGSLSPGAEVSEGDGGTAIIT
                     WNSTKNAELRFYTENRVTLLTMFGQFWIIIAVVLLLPFLFTWQRKAG"
     gene            complement(6678..7574)
                     /locus_tag="PK154_04815"
     CDS             complement(6678..7574)
                     /locus_tag="PK154_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW10416.1"
                     /translation="MNDKAACGLFDSGHRACGGCGAAMAARLMLDAAGKDTIIVSSTG
                     CMEVFSTPYPETAWKVPWIHSLFENNSAVASGIVAALAKQGRKERVVAIGGDGATFDI
                     GILCLSGAFERGHDFTYICYDNEAYMNTGIQRSGATPYDASTTTSPAGSCSFGNKRPK
                     KDLPLILAAHGAPYVATASIAYPPDFKKKVEKAINTPGPCYIQVHAPCCTGWGFEGEK
                     TIAIGKLAIETGLWVNYEMENGVVTKVKKVVRKPVEDYLVEQKRFRHLFKPKKNETEI
                     AKIQALADRNAEKFGIDIKLKK"
     gene            complement(7571..8728)
                     /locus_tag="PK154_04820"
     CDS             complement(7571..8728)
                     /locus_tag="PK154_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPW10417.1"
                     /translation="MLQITEGSLAVADAVGKCRPQVIAAYPITPQTHIVEALAEMVAN
                     CTIDAEYITVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNCAGMRLPVVM
                     TIANRAMGAPLSIWNDQQDSISLRDSGWLQFYAEDNQEATDLHYLAYRVSEDHNILLP
                     SFVCFDGFILSHTYEPVDILSQEEADRYLPRFRPFQRLDADDPISFGLFAAPDYYMEF
                     RYEIDQAAKKALSVFPKAGREFAELFGRDYSGLVESYRMEDADCALVAMGSLCGTVKD
                     AIDDLRDAGRKVGLLKIRTFRPFPAEEVKKALSGVSRVAVLDKNISLGSKGAVALEVK
                     DALYGSQIPVHDYIIGLGGRDVQKKDIARVVTLAEEGRGDLYYGLREEVLK"
     gene            complement(8730..8990)
                     /locus_tag="PK154_04825"
     CDS             complement(8730..8990)
                     /locus_tag="PK154_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW10418.1"
                     /translation="MALGTGCTARPGKSRNNRTGSWRVFRPAFIHERCTSCGMCRIVC
                     PEGCIGENEEGLPVPDYDYCKGCGLCAEECPSEAIEMKKEEK"
     gene            8898..>9187
                     /locus_tag="PK154_04830"
     CDS             8898..>9187
                     /locus_tag="PK154_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10419.1"
                     /translation="MDECRPEHPPRPGPVVARFPGSGSAACAERHAARRGNQGLEALV
                     GQLEGFRGADRGVDARAGNVLFKGERPAGAVRDLQVRGAEGQRQVRGQQGLEP"
BASE COUNT         1956 a         2674 c         2569 g         1988 t
ORIGIN      
        1 cacctcctct ctgaccgcct ccggtggctg cccctcctga cgttcctcgt ggtcaatccc
       61 tccttcatct ggcaggtgat cctcgggaaa acctcagggg ctgtcctctg ggatagtacc
      121 cgcacgatcc cggacaccat tcacgaatat ccggcgttct cgttcgtatg gggcgatctc
      181 catgcccatg ttgtctctct cttcaaccag gtcttccttc tcttcctgtg tgtatatgcc
      241 ctcacccact ggaagatgat gactccccgg caccgctttt tgctcgtgat actgtccgcg
      301 ctttctcttg gttccatgcc cctgatcaat acctgggatg tgctcctcta tgccccggtc
      361 accgtgctct tcgggctcct catctggcat gagaacaggg agagcgtcgg ggaaggcggg
      421 tccgggacac ccccggctat cggacccttg gaacggatta tggcccgggc atacagtttc
      481 cttctcctcg tcccgcccct ctccctcctc tgctacctcc cctactacgt ccagctcaag
      541 agtggtggga tacaggggat aggcatcgtc cctaccccaa gccctgtcct cgccttcctc
      601 cttgtccacg ggttgttcct catcctcttc ctggtgtatt tgagaaagga catcatccgg
      661 atgccactgc tccttctcgt ccccgtcccc ttcattctgg gggggtacgc tgcagcagga
      721 atcgcggccc tgccgctggc atacttcctt gccaggaggc tccggacccc cgctgaaatc
      781 ctcgcaatct gcggactttc cgtgatcata ttctgtgaat ttttctacct gaaggataac
      841 atgggggagg tatattaccg gatgaatacg gtcttcaaat tctatcttcc cgcctggatc
      901 ctgatggcat cctccgggtt tgcaatgctt gctgtcatgc tggagcagcc ggtgtcccat
      961 ctgaagatca gcaagggtct gaaacgagcg gcactcatcg gcgtcacagc gctgcttctt
     1021 acggcacccc tgatcatccc attcgaatac agctcccgcg atgccaccct gaatggactc
     1081 gcctggcttg acacgaccca tcctggcgat gcagcggcga tcgcgttcct caggtcactt
     1141 tcaggcagct acgggattgt ggaggctgaa gggggagatt acgggtacta ctcgcggata
     1201 tcatcatcga ccggaattcc tgccatcatc ggcatgccat ttcacgagta catgtggcgg
     1261 gctgacacgt ggtatgggga acgagtaaat gatatccggc tgatctatga ggaccctgca
     1321 cagaccgtcc ctctcatgag gaagtataat gccacgctcc tctatatcgg ggacccggag
     1381 cgggagcggt atgcagtgag ggcggaggaa gccggccttc cgctcctcta taaccagtca
     1441 ggggtacaga tctacctcct ctccgcctga acagcccggc tgccggtaca ctctcacaaa
     1501 catttattgc cattcctgtt gattttatat agctacatcg tcgtcactga ctgatgagtg
     1561 atgagggaga ctacgctact cctgaatgag gtgagttatg gcaaagacgt acgtaaaatt
     1621 ccaggtttct gaagagattc agaacaaggc gctcgaagcc cttgagattg cacgggatac
     1681 cgggaagatc aagaaagggg caaacgaggc caccaaggca atcgagagga gtcttgcaac
     1741 gctggtcctt attggtgctg atgttgagcc ggaagagatc gtgatgcacc tcggcccgct
     1801 ctgtgaagag aagaagatcc cctttgtgta tatcaacaaa cagaatgata tcggggccgc
     1861 aagcggcctt gaggttggct ccactgctgc agcgatcgtc aagcccggga aggccaagga
     1921 tctcatcgac gaggtcgcca aacagatcag cgacctgaga gcgtgaacag accatggcag
     1981 atgaaggaac gcctgcagag gtcatcgaag tgatcggatc caccgggatg cacggcgaag
     2041 ccatgcaggt gaaatgccgg attctcgacg gtccaaacaa ggggcgcatc atcacccgca
     2101 atacggtcgg acccatccgc gagggtgacg tgctgatgct ccttgagacc gaacgcgagg
     2161 ccaagaaact ctcacggcgg tgaacgtact atggtagaac agaaaaactg cagtttctgc
     2221 ggtgaaccaa tcgagcccgg gaccgggaag atgtttgtca ggaaagacag taccatcttc
     2281 tacttctgca gctcgaagtg ccagaacaac ttccgactcg gcaggactcc ccgcagggtt
     2341 gcctggacaa aagccgggcg aaaggcgctc ggaaaggagt gatgcggcat ggaccggacc
     2401 tttgtgatgg tcaaacctga cggggtagcc aggggactga tcggggaaat ggtcagccgt
     2461 ttcgaaaaaa aaggcctcag gctggtcgct gcccgtctcg agaagctccc tgaaaagcgg
     2521 gtgatggaac agtacaggga acacctcgaa aaaccgttct tcccatcgct ccggcactat
     2581 atcaccagcg gcccggtatt cctgatggtc tgggagggga aatcagccgt cacggtcgtt
     2641 cgggcgcttg tcggtgccac aaatccggcc gaggccgccc cggggacgat ccgcggtgac
     2701 ttcgcacttg atatcggcag gaacgtgatc catgcctccg attcgccgga aagtgcaaag
     2761 cgtgagatcg ccctccattt cacccaggaa gatttctgct cgtacaagag ggtcgacgaa
     2821 gccgttttgt acgaataagt atcaccggaa tcatcccctt ctttaacaga tctgataacc
     2881 actcttctgg tcacatacct ttctgacgaa ccccgcaggt gcggttttca tgcaggaatg
     2941 gcgtggatca tttttcaggg tgtatacccc tgtctcaaag agagccatgc atggttgccc
     3001 ccctgttatg atggaacctt tcgtatgccg gatggggggg aaggcaaacg ctcttccctt
     3061 cagagggcac accatcccct atggccggcg aaatggtcac gtttgcgctc ctctcgctct
     3121 cgtcgatcat tattattgtg aatccgcttg gcgccaccct taccttcgtc tccctaacga
     3181 gctcgcttga tcgtgaaaca aggctgaagg tggcagtgga atcctgccgg tatgcgctcg
     3241 cgatcctcat cgcctttgcc ctggcgggag ggatcatcct ccggctcttc ggcatcacga
     3301 tcgatgcatt ccgtattgga ggcggcatcc tcctgttcgt gatcgggatg gagatggtct
     3361 acgcccgcac ctcacggtct aagctgaccg caacggaaaa atatgagagt atggatgcag
     3421 aggatatttc agtcaccccc ttagcaatac ccatgattgc aggacctggt gcaatcacca
     3481 cgacaatcgt tctgatgaat gaagcgacgg gatcagtacc gcggattgcc ctcattctcg
     3541 tctcgatcgt gatcgcaatc cttctcacct acctgatgat ggtacgatca gatcagatcg
     3601 tcagtcgcat cggacagaaa gagttccggg ctatcaaccg gctgatgggg atgcttctga
     3661 ttgccattgc ggtccagttc gtgatcgccg gactcactgc ggcatttccg gtcctgtcag
     3721 ggatctgaac aaaggctcca gacacagaga tcaacaagga gaacaccatc atatgcccat
     3781 aaaaattgcc ggagcccaga tcgggagcag gtgggaggat ccacagcaaa gtctgcaaaa
     3841 ggcggaacag tccatcagga gagccgttaa tgaaggagcc tcactgatct gttttcccga
     3901 gcaatacccc acaggatggg acccccggtc tgtccggcat gtcgaggaat gggacggccc
     3961 aatcgtctcc cggttccgtc accttgcaca ggaatatggt attgcaatac tcggctcatt
     4021 ccgtgagcgc tctgatccta aaccgaggaa tacctgcatc gttctcgacc cacacgggga
     4081 gattgccgct acctatgcga agtctcacct gttcaccccg gcacacgaag acgaatacta
     4141 ctcccctggt acaaacccgg gaatcttcag actctcgaat gtcccctttg gtatcgccat
     4201 ctgctatgac ctccgttttc ccggactttt cgggacgtac gcaagggcag gagtctgctg
     4261 catgctggtc cctgcagcat ggcctgcgtc ccggatcggc cactgggagc tcttactcag
     4321 ggcacgggcg ctcgaatacc agatgtatgt catgggggtg aacccgacgg ggatgactcc
     4381 ggtagagacg tattgcggca gatcacttgc catcgatccc tccggaaccg tggttgcaga
     4441 agcagggggg gaggaggcgc tgatccctgc catcatcgat ccggaggtgg ttcatcgtgt
     4501 ccgcgaggcg ctccccgtat accagaacgg tctgttggat gatttttccc gaaacccggc
     4561 ctgaccaaag gaatgggatc agttgcaata ccggatgcgt atatctgcga tgaccactca
     4621 gatctagagc atgtatcgcc tcacctgcgt gcaatgcggc cggtcctatc ctcctgatga
     4681 gatcatctat acctgccgtt cctgcgggca cctgctcttt gtggagtacg atctcgactc
     4741 cctcaccatc aggcgtgaag agtgggatcg tcgtccgatc tctgtgtggc ggtaccgtga
     4801 gctccttccg gtcaccatcc caccagttac cctgaaggaa gggggtaccc cgctctacca
     4861 cctggaacgt atcggagaag agatcgggct cccacacctg tatgcaaaac atgaagggat
     4921 gaatccgacc gggtcgttca aggacagggg aatgacggtc ggggtgagta tggcactcca
     4981 gctcaacaaa tcgacggtgg cctgtgcaag taccgggaat acctcggcaa gccttgcggc
     5041 ttatgcagca aaggccggga taccggctgt cgtgctcctc cctgccggca aggtggcgct
     5101 tggaaaagtg gcacaggccc tgatgcacgg ggcacgtgtc atctcgatca ggggtaattt
     5161 cgataaagcc ctcgagatgg tgcaggaact gtgcatcagc cacgggctct acctcctgaa
     5221 ctcgatcaac cccttcaggc tggaagggca gaagaccatc gggttcgaag tgatcgacca
     5281 gctcgggagc gtaccggatc ggatcgtcct cccggtcggg aatgcaggga atatctctgc
     5341 cgtgcacaaa ggactcggtg aactgcacgc acttggcctc atcgagcgct acccgatgat
     5401 gaccggcatt caggcgaagg gctcaagccc ggtcgtcagg gcgatacggg aaggactccc
     5461 ggaagtgatt ccggaaggag agccggagac cgtggccact gcgatacgga taggagctcc
     5521 ggtcaacgcc gagaaggcac tcaccgcgat ccgaaagacc ggcggtacag ccgaatgggt
     5581 caccgacgat gagatcctcg cgatgcagag ggatctcgca cgaaaagagg ggatcggggt
     5641 tgaaccggca tcggcggcat cggttgcagg tgtacgcaaa ctcgctcttg aaggagtgat
     5701 cgatccgagt gaacgggtcg tctgtgtggt gaccgggcac ctgctgaaag acccggagac
     5761 ggtgataaag caatgcaagc cacccaccga gatcgatgcg gacgtgcaat cgctgctctc
     5821 tgcattgcga tcctcctgat ccctgcatca gtcctggccc tcagcgccgg gtatgagatc
     5881 ctgccgaacg ggacagcata ccgggcatcg gtcgagatcg tggatgccga ccgttattct
     5941 ttttctgaga cgggattcct gggagagaga attcctgtcc aggtaaacga tgtctcgctc
     6001 tcgggcgatt gtccccgcaa ttcctgcagt tacaacagtt ctgaccggtt caccatcacc
     6061 tttcccaaag ggaattatac cgttcactat accggatcgg tccgggataa ccatttcctg
     6121 gccacgtttc ccgagccata cttggtgcag gtgatcctcc cgcccccatt cgatgtcagg
     6181 aaccggttta tcggctccct cagtccaggg gcagaagtga gcgagggaga tggcggcacg
     6241 gccatcatca cgtggaactc gacaaagaat gccgagctcc ggttttacac tgagaacagg
     6301 gtgaccctgc tgaccatgtt cgggcagttc tggatcatca ttgcagtagt gctcctgctc
     6361 ccgttcctct ttacctggca gaggaaggcg ggatagagcg gggggctccc ctccacgtta
     6421 tcccaaggat ctttcctttt tgaataacgc cctcgaggag ggcagattca ggtcttcctt
     6481 acaccgccag ggggcttctg atccgagagg aaccggcgga gcggcattta ccatggtcct
     6541 aatccctgca tataaagagt ttcgtggtcc atgagactgc gaagaccagg tattttgaaa
     6601 cgtagtctga tgtcgggatg agtggcaaaa aaagaatgag aagcacttcc gattgcagcg
     6661 attttgcaga aaaaaaatta ttttttcagc ttgatatcga tcccgaactt ttcggcgttc
     6721 cggtcggcaa gggcctggat cttggcaatc tcggtttcgt tcttcttcgg tttgaagagg
     6781 tggcggaagc gcttctgctc gaccaggtag tcttccaccg gctttcgcac taccttcttc
     6841 accttcgtga ccacgccatt ctccatctcg taattgaccc agaggccggt ctcaatggca
     6901 agcttgccga ttgctatcgt cttctctcct tcgaatcccc atcctgtgca gcagggtgca
     6961 tggacctgga tataacaggg tcccggggta ttgatggcct tctcgacctt cttcttgaaa
     7021 tcaggaggat aggcgatgga tgccgttgcc acgtaagggg cgccgtgggc tgcaaggatc
     7081 aggggaagat ccttcttcgg ccgcttgttg ccaaacgagc agctacccgc ggggctcgtg
     7141 gtggtgctgg catcgtaggg ggtcgcccct gatcgctgga tgccggtatt catgtaggcc
     7201 tcgttgtcgt agcagatata ggtgaaatca tgaccccgct caaatgcccc cgagaggcag
     7261 aggatcccga tatcaaacgt tgcaccatcc cccccgatcg caacgaccct ttccttcctc
     7321 ccctgctttg cgagagcggc aacaatccct gatgcaacgg cagagttgtt ctcaaagagc
     7381 gagtggatcc aggggacctt ccatgccgtt tcagggtacg gcgtcgagaa gacctccatg
     7441 cacccggtcg atgagacgat gatcgtatcc tttcccgctg catccagcat gagccgggct
     7501 gccatggcag caccgcagcc accacacgca cgatgacctg aatcaaagag cccgcaggcc
     7561 gccttgtcgt tcatttcagt acctcctcgc ggagcccgta atagaggtcc cctctgcctt
     7621 cctcggcaag cgtaacaacc cgggcgatat ccttcttctg cacatccctg cctccaagac
     7681 cgatgatgta atcatggacc gggatctggc tcccgtagag ggcatctttc acttcaagtg
     7741 ccacggctcc cttcgacccg agagatatat tcttgtcaag gactgcgacc cggctcacgc
     7801 cggataaggc ttttttcacc tcttctgccg ggaacgggcg gaaggtccgg atcttgagca
     7861 ggccgacctt tctgcctgca tccctcagat catcgatggc atccttcacc gtcccgcaga
     7921 gcgaccccat tgccaccagg gcacaatccg catcctccat gcggtagctc tcgacaagcc
     7981 cgctgtaatc cctgccaaag agctcggcaa actctcttcc tgccttcggg aataccgaaa
     8041 gggctttctt tgcggcctga tcgatctcgt agcggaattc catgtagtag tcgggggccg
     8101 caaacagacc aaagctgatg ggatcgtcag catccagccg ctggaatggc ctgaatcgcg
     8161 ggagatagcg gtccgcctcc tcctgggaga ggatatcgac cggttcatag gtatgggaga
     8221 ggatgaaccc gtcgaagcag acaaacgatg ggagaaggat attgtgatcc tcggagaccc
     8281 ggtaggcaag gtaatggaga tcggtcgctt cctggttgtc ctctgcatag aactgcagcc
     8341 agcccgaatc ccggagcgag atcgagtctt gctggtcgtt ccatatggag aggggtgctc
     8401 ccatcgccct gtttgcgatg gtcatgacga ccgggagccg cattcccgcg caattgaaac
     8461 agacctcgaa catcaatgcc agcccctggg aggtggtggc tgaatagacc ctcgatccag
     8521 cagcgctcgc accaaggcag gccgagagcg cagagaactc gctctcaacg gtgatatact
     8581 cagcatcgat cgtgcagtta gccaccatct cggccagcgc ctcgacaatg tgggtctggg
     8641 gggtgatggg gtaggcggca atcacctggg gtcggcattt ccccactgca tctgccaccg
     8701 caagggatcc ctcggttatc tggagcatgt cacttctcct ccttcttcat ctcgatcgct
     8761 tcggacgggc attcctccgc acagagcccg caccccttgc agtagtcata atccggaaca
     8821 gggagtccct cttcgttctc accgatacag ccttcagggc agacgatcct gcacatcccg
     8881 cagctggtgc atcgttcatg gatgaatgcc ggcctgaaca cccgccacga cccggtcctg
     8941 ttgttgcgcg atttcccggg tcgggcagtg cagcctgtgc cgagcgccat gcggcccgtc
     9001 gcggaaacca aggtctcgaa gccctcgtcg gccaactcga aggtttccgc ggtgccgacc
     9061 gtggagtaga tgcccgtgcc ggaaacgtac tctttaaggg tgaaaggcca gctggtgcgg
     9121 tacgcgatct ccaggtccgc ggggccgagg gccagcgtca ggtccgcggc cagcaggggc
     9181 ttgagcc
//
LOCUS       DAOOCO010000061         9106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7217_length_9106_cov_5.668876, whole genome shotgun sequence.
ACCESSION   DAOOCO010000061 DAOOCO010000000
VERSION     DAOOCO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7217_length_9106_cov_5.668876"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK154_04835"
     CDS             complement(<1..314)
                     /locus_tag="PK154_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPW10420.1"
                     /translation="MSVARFWRKIPQRYNLIGTRCTTCGRYFFPPRSFCPECRRAGKI
                     VEHQFAGNGKIVTFTVIRTASEQFEQLTPYVFAIIELDEGPRISAQIVCSPDDARIGM
                     RV"
     gene            complement(318..1481)
                     /locus_tag="PK154_04840"
     CDS             complement(318..1481)
                     /locus_tag="PK154_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HPW10421.1"
                     /translation="MRDVAVIGAGCTTFGEKWDTSFRNLFVEAGALALEDAGLAGEQI
                     DALYVGNMSAGRFIEQEHIGALIADYAGLASHHVPATRVEAACASGGLSFRQAVIAVA
                     SGLEDIVVAAGVEKMTDVGTGASVDALAGAADREWEGFAGATFPGLYAMIARDYMHRY
                     PLTREQLGLVAVKNHYNGARNPIAQFQQEISLETVLKSSLVADPLRLFDCSPITDGAA
                     AVVVAPLDRAREFTDTPIRVLASAQASDTITLHDRRDISTLDATVAAGQRAFRMAGLS
                     PSDIDMVEVHDCFTIAEICAIEDLGFCRKGEAGRITEEGVTALNGDLPVNTSGGLKAC
                     GHPVGATGIKQVYEITQQLRGEAGRRQITGAQIGMTHNVGGTGATVAVHILGV"
     gene            complement(1478..2530)
                     /locus_tag="PK154_04845"
     CDS             complement(1478..2530)
                     /locus_tag="PK154_04845"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HPW10422.1"
                     /translation="MVGIITYGVYIPRYRIKVEEITKVWGANAEDVTGGLGVYSKSVP
                     DLDEDAATIAVEAARNALRRREIDPCDIGAVYVGSESHPYAVKPTACTVGEAIGATPC
                     MTAADYEFACKAGTAAIQTCMGLAKSGMIRYGFAIGSDVAQGAPSDALEYTAAAGGAA
                     FLIGRDDPIALIHRTCSFTTDTPDFWRREGQDYPRHGGRFTGEPGYFKHIEGAVRMIF
                     DQSGKGPKDYDYAVFHQPNAKFPQRVAKILGFSPDQIRPGLVVPRLGNTYSGSSMIGL
                     AATLDIATAGDRIFVASFGSGAGSDAFDIEVTDQIEGDSFRLTAAPGVEQLLKDPIYI
                     GYAQYARHKGKIVMQK"
     gene            complement(2524..3234)
                     /locus_tag="PK154_04850"
     CDS             complement(2524..3234)
                     /locus_tag="PK154_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW10423.1"
                     /translation="MKSDVRYQLAEKMAGEITLSESPGKALKKWRMSFNIPQGVLCEH
                     LQVSPSVISDYESGRRKSPGTAVVGKIVDTILTIDEANGGKFIKKFAKILYTDIDDNV
                     IYDIFDYASPVPLTRLSEVLSCKPLCGSLNQSIFGYTIVNSLNAITQLSANEFNRIYG
                     WSTERALIFTDVTSGKSPMVAIRVTPFKPRCVVLQGIEPELVHPLVPRMAERDHITVL
                     CTMMGIDEIVDILREERW"
     gene            3350..4015
                     /locus_tag="PK154_04855"
     CDS             3350..4015
                     /locus_tag="PK154_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HPW10424.1"
                     /translation="MILPEDLQCLKEIALKGGLKGPVWVSSQSLGGALGTSPQTASRR
                     LQSLERQQYLSRSVNPDGQYVTVTRSGEERLRHEYADYCRVFGSGREEFVLQGTVISG
                     LGEGRYYMSLEPYVQQFSQKIGFAPYPGTLNIRLEPSSMQVRKRLDQLEWIRIHGFSA
                     EGRTFGDARCLPCSIAGIPCGIVIPGRTHYPDDVLELIAPVALREALRINDMDEVKVE
                     VAP"
     gene            4012..4689
                     /gene="ribB"
                     /locus_tag="PK154_04860"
     CDS             4012..4689
                     /gene="ribB"
                     /locus_tag="PK154_04860"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HPW10425.1"
                     /translation="MMEEALTALRQGKFILLYDFDDREGETDFAIRSDMVRPADIALM
                     RKDGGGLICTAIHPKAAACLGLPFASDMLSTCTNFAEAVGDIPYDRKNHSSFSLWVNH
                     RNTFTGITDNDRALTITAIADEVKRSLNGGGCDFHGRFRSPGHVAILRAAEGLLSLRR
                     GQTELSVAMAIMAGITPAVTVCEMLDDQSGLALSKEDARDYARKHQLVFIEGTDVIEA
                     WERFSSG"
     gene            complement(5079..5240)
                     /locus_tag="PK154_04865"
     CDS             complement(5079..5240)
                     /locus_tag="PK154_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10426.1"
                     /translation="MDEKQRAELIKNLDLKVLKEEAKARGIKPGRCPTKSSIAKLLPD
                     EALLSLAKK"
     gene            complement(5290..5883)
                     /locus_tag="PK154_04870"
     CDS             complement(5290..5883)
                     /locus_tag="PK154_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HPW10427.1"
                     /translation="MAAPERPSFARREGTFEREPARRVFAGELRETRIHFREGEDEKS
                     PTYVLLPTGERCNRVFLVGTLTEKKKQGEQNMFYRARVVDPTGTFFIMAGSYQPEAMQ
                     QIARIEPPAFVAVAGKPNVYETPDGATLISVRCESVTPVEKDTRDRWVLDCALSTLDR
                     IDNMGTTKYGERARLEYHSDPAVFRKMVFEALSQLSL"
     gene            complement(5883..7118)
                     /locus_tag="PK154_04875"
     CDS             complement(5883..7118)
                     /locus_tag="PK154_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HPW10428.1"
                     /translation="MDYSSISERISLKVSAKGHQADRTKIEAKLRRLMEEFGVPPQEA
                     ERTVLNELARDLSIPDLLPGTAGTAGEERQLRQLSSGDWATIEAKVVSLSSSPSAAIS
                     QTGILADPTGAMRFVVWAKANAPRLSAGSWYRFESVVVDEYRGTPNLKIHSGTTIKEI
                     SRETPLIPEITPLAELTPGIGSVRGKFIQEWDVSHDRMLQSGLLGDESGTVKFVIWKE
                     EGKERLNPGTVYSVYYALIDEYQGRISLNITTATTVPEEGDIPVSGGEGEFAGALVHI
                     APGSGLIKRCPVEGCNRVLSRQNYCPVHEIQPGFLYDLRIKGWLDNGKETREILVQRE
                     VTEKLTGISINDAKELAENNPLGMDEVFLRMRERILGRYFTCRGREIDQRILVSACAF
                     QHFEPGEIPTLLNRAGGAL"
     gene            7237..7431
                     /locus_tag="PK154_04880"
     CDS             7237..7431
                     /locus_tag="PK154_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPW10429.1"
                     /translation="MELTADSTIYDLLKAKPESAEVLFKFGMGCVGCAIARGETIREA
                     AMVHGIPLEELTAALGIKEA"
     gene            complement(7467..8348)
                     /locus_tag="PK154_04885"
     CDS             complement(7467..8348)
                     /locus_tag="PK154_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HPW10430.1"
                     /translation="MRYYLRENTLFVRGSYRSLSTGVGGGIGTVSTLLNHTLRPDWDG
                     QEPLRELALVASREGLPADFFGLLTTVRMQHLCILQYDFVTVFISAGIHKESPGTINI
                     IVCSSQGMSDAALAGAIITATEAKSEALRGLGHPFYGTPTDAVIIASEGVVVHPSAGI
                     HTEVGHRIHAAVLFGIPEALRRHEGVVTREASSYFIFSRFGGDHWVEWLPKACPYYPC
                     HFEGQRCEFCYCPFYPCGDESLGQWVRSSSRNGPVWNCAGCTLLHQPGIADYLKQNPE
                     ASLRELKMRQREKQNEA"
     gene            8434..8670
                     /locus_tag="PK154_04890"
     CDS             8434..8670
                     /locus_tag="PK154_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10431.1"
                     /translation="MNQNQNKLANPLFWVSIPPGSSKTGEVVFGVKASTELFMLMLLD
                     GKGQVLLTEPVRPVTTGPYLSSVEDALPNNTYFS"
     gene            complement(8894..>9106)
                     /gene="nrdD"
                     /locus_tag="PK154_04895"
     CDS             complement(8894..>9106)
                     /gene="nrdD"
                     /locus_tag="PK154_04895"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPW10432.1"
                     /translation="IMCPLDHCHCSHEIISGIEYCPLCGEPVCPDCGSHDVVQISRVT
                     GYLQEVSGWNAGKQQELKDRKRYTVA"
BASE COUNT         2062 a         2475 c         2379 g         2190 t
ORIGIN      
        1 tttaccctca tcccgatccg ggcatcatcc ggtgagcaga cgatctgcgc tgagatccgt
       61 gggccttcat caagttctat gattgcaaaa acgtatggcg tgagctgttc aaactgctcg
      121 ctggccgtgc ggatcacggt aaatgtcaca atttttccat ttcccgcaaa ctggtgctca
      181 acaatctttc ctgccctgcg acactccggg cagaaggagc gcggcgggaa gaagtaccgc
      241 ccgcaggttg tgcagcgagt cccgatcagg ttgtaccgct gggggatctt tctccagaaa
      301 cgagcgaccg acattcttca cacccccagg atatgcacgg cgaccgttgc tccggtgcca
      361 ccgacattat gcgtcatccc aatctgggca cccgtgatct gtcggcgtcc agcctctccc
      421 ctgagctgct gggtgatctc gtatacctgc ttgatcccgg tcgcgccaac aggatgcccg
      481 catgctttca gccctccgct cgtattgaca ggcaggtctc cgttcaacgc agtaacccct
      541 tcctcggtga tccggcctgc ctcccctttc cggcagaatc caagatcctc aatggcacag
      601 atctcggcga tggtaaagca gtcatgcacc tctaccatat cgatatctga aggagagagt
      661 ccagccatcc tgaacgcccg ctgccctgcg gcgaccgttg catcaagagt actgatatcc
      721 cgtcggtcat ggagggtgat ggtatcgctt gcctgggcac ttgcgagcac cctgatgggg
      781 gtatcggtaa attcccgggc acggtcgagc ggggctacca ccaccgcggc tgcaccatca
      841 gtaatcggcg aacaatcgaa aagccggagg gggtccgcga caagcgatga cttgagaacc
      901 gtctcaaggc tgatctcctg ctggaactgg gcaataggat tcctcgcacc gttgtagtga
      961 ttctttacgg caacaagacc gagctgctca cgggtgaggg ggtatcggtg catgtaatcc
     1021 cttgcaatca tagcatagag acctgggaac gtcgcaccgg caaatccttc ccattcgcga
     1081 tcagcggcac cggccagggc atccacgctt gcacctgtgc cgacgtcggt cattttttca
     1141 acccccgctg ccaccacaat atcttcaagt cctgacgcta cggcaatcac tgcctggcgg
     1201 aaagagagcc ctcccgaagc acacgctgct tcgactcgtg tcgctggtac atggtgggat
     1261 gcaaggcccg catagtctgc gatcagtgca ccgatgtgct cctgttcaat gaaacgccct
     1321 gcactcatat tacccacata cagggcatct atctgttcac cggcaagccc tgcatcttca
     1381 agcgccagcg cacctgcctc cacaaagagg ttccggaacg aagtatccca tttctctccg
     1441 aatgtggtgc accctgcccc gatcactgct acgtccctca tttctgcatc acgatctttc
     1501 ccttgtgtct ggcatactgt gcatacccga tgtagattgg atctttcagc aactgctcaa
     1561 cccccggcgc tgcagtcagc cggaacgaat cgccctcgat ctgatcagtc acttcgatgt
     1621 caaatgcatc gcttccggca ccggagccga aggaggcaac gaaaatacgg tcacctgctg
     1681 tcgcgatatc cagtgtcgcg gcaaggccga tcatggaaga gccggagtac gtattgccaa
     1741 gccggggcac tacaagtcct ggcctgatct gatcagggga gaagccgagt atctttgcca
     1801 cgcgctgggg gaactttgca ttcggctggt gaaaaaccgc ataatcataa tccttcgggc
     1861 cctttcccga ctggtcaaag atcatgcgga ctgctccttc gatgtgtttg aaataacccg
     1921 gctccccggt aaaacggccc ccatggcggg ggtagtcctg accctcccgt ctccagaaat
     1981 caggagtgtc cgtagtaaat gagcaggtcc ggtgaatgag tgcgatggga tcatcccttc
     2041 ctatcaggaa tgctgcaccc cctgctgcgg ccgtatactc cagtgcatca cttggtgcac
     2101 cctgtgcaac gtctgatcct atggcgaagc cataccgtat catcccgctc tttgcaagcc
     2161 ccatgcaggt ctggattgca gctgtacctg ccttgcaggc aaattcgtaa tcggcagcgg
     2221 tcatgcaggg agtagctccg atcgcttcac caactgtaca cgcggtcggc ttgacagcgt
     2281 acggatggga ttcagagccc acataaacgg ctccaatatc gcagggatca atttccctgc
     2341 gcctcagcgc gtttcgtgca gcttcaacag caattgtggc agcgtcctca tcaaggtccg
     2401 ggactgactt ggaatacact ccgagtcctc ccgtcacatc ctcggcattt gcaccccaga
     2461 ccttggtgat ctcttccact tttatccgat accggggaat gtacaccccg tatgtgatga
     2521 ttcctaccat cgttcttccc tcaaaatatc cactatctca tcgatgccca tcattgtgca
     2581 tagtacggtg atatggtccc tctcagccat ccttggtacc agagggtgga cgagctcagg
     2641 ctcgattccc tgcagtacaa cacagcgtgg cttgaaggga gtgacccgga ttgccaccat
     2701 cggggatttt ccagaagtta catcagtaaa gatgagtgcc ctctcggtac tccagccgta
     2761 gatacggttg aactcgtttg cagagagctg ggtaattgcg ttgagactgt tgacgatcgt
     2821 ataaccaaat attgactggt tcagcgaccc gcagagtggc ttgcaggaga gcacttctga
     2881 aagcctggtg agcggtaccg gactcgcgta atcaaaaatg tcataaatga cattatcgtc
     2941 gatatcggta tacaggatct tcgcaaattt tttgataaac tttccaccgt ttgcttcatc
     3001 gatggtaagt atcgtgtcaa caatctttcc gacaacagca gtaccgggac tttttcttct
     3061 cccgctttcg taatcactga tcacggaagg ggagacttga aggtgttcac agagcacgcc
     3121 ctggggaatg ttgaaactca tcctccattt cttcagggct ttccccggag attctgaaag
     3181 cgttatctcc cctgccatct tctcggcaag ctgataccgc acatcagatt tcatgcctca
     3241 ctatgagggc aggggggttg ataaaagtaa cgaacagggc ttcgacattc cacgaaaata
     3301 cctgatatag atactcataa ataataagca tctcaacttt gataaggata tgatcttgcc
     3361 cgaggatctc cagtgcctga aagagattgc cctgaagggc ggcctgaaag gaccggtctg
     3421 ggtctcttcc caatcgcttg ggggcgcgct tggaaccagc ccgcagactg catcacggcg
     3481 actccagtcc cttgagcgac aacaatatct ctcccgttca gtcaatcccg atggtcagta
     3541 tgtcacggtt acgaggagtg gagaggagag attgaggcat gagtatgcag attactgccg
     3601 agtattcgga agcgggaggg aagagttcgt cctccagggg accgtcatct ctggtcttgg
     3661 agagggccgg tattacatga gtctcgagcc ctatgtgcaa cagttctccc agaagatcgg
     3721 atttgccccc tatcccggga ccctgaatat caggcttgaa ccatcgagca tgcaggtccg
     3781 gaagaggctt gatcaactgg aatggatacg gatccatggt ttttcagctg aaggccgtac
     3841 gtttggggat gcccgctgtc ttccctgtag tattgccggt atcccctgcg gcattgtaat
     3901 cccgggaaga acccattacc ccgacgatgt cctcgaactg attgcgccgg tcgcgctcag
     3961 ggaagcattg agaataaatg atatggatga agtaaaagtt gaggtggcac catgatggaa
     4021 gaagcactga cagccctgcg ccagggaaag tttatcctgc tctatgattt tgatgaccgg
     4081 gaaggtgaaa cggattttgc cattcgttcg gatatggtgc gccctgcaga tattgcccta
     4141 atgaggaaag atggaggggg gctgatctgc acggctatcc atccaaaggc agcagcgtgc
     4201 ctcggtctcc ctttcgcgag cgatatgctc agcacctgta ccaattttgc agaggctgtg
     4261 ggggatatcc cgtacgatcg aaagaaccac tcatcgttct cgctctgggt gaaccatcgg
     4321 aacacgttta ccggaatcac cgacaacgac cgtgctctta ctatcaccgc gattgcggat
     4381 gaggtgaaac ggagtctgaa tgggggaggg tgcgacttcc atggccggtt cagatcccca
     4441 ggacatgttg ccatactcag ggctgccgag gggttgcttt ccctgagaag aggccagacg
     4501 gagctatcgg ttgcaatggc aattatggca ggcatcactc ctgctgttac tgtctgtgag
     4561 atgctggacg atcagtcagg ccttgcccta tccaaggagg atgcccggga ttacgcgagg
     4621 aaacatcaac tggtctttat cgaaggaact gatgtgatcg aggcctggga acgtttctct
     4681 tccgggtaac attttaccac ttttatctgt tctttagtct gttatgatag atcttaagaa
     4741 atcctgaaaa gacagatgaa gactgatcag gattttttat tcatgagcct ttgaggagaa
     4801 attcctggct tttgaatgac ccgcaatgtc agtcatacct tgctcaactg aggaacagcc
     4861 ctgaatgggt gaagctaatt ttttaccagg gaacaggggg tgagctatgg cagggcggca
     4921 tttccaatct tcgttgaacc tggaaacgat gctcacatgg ggtagaagtg tatcgggcag
     4981 gatgaaaact gaggcgctcc ttatccccga agagtggatc attcaccctg aagcagagct
     5041 cctcccggta cagattatga ggggtggcag tcagaatctc attttttggc gagagagagg
     5101 agtgcttcgt ctggtaataa ttttgcgatt gaagatttcg tcgggcatcg tcccggtttg
     5161 atccccctgg ctttggcctc ttccttcagc actttcagat ccaggttttt gatcagctcg
     5221 gctctttgtt tctcatccat tgttgatcgg acgaagatat ctgcccggaa aaataaaggt
     5281 tactgctgtt tacagactga gttgcgagag tgcttcaaac accatcttcc ggaatactgc
     5341 cgggtcggaa tggtactcta atcgagctct ttcaccgtat ttcgttgttc ccatgttgtc
     5401 aatacggtca agcgtggaaa gggcacagtc aagcacccag cgatctctcg tatccttctc
     5461 aaccggggta acggactcgc acctcactga tatgagggta gctccatccg gggtctcgta
     5521 cacatttggt ttcccggcta cagcgacaaa tgcagggggt tcgatccggg cgatctgttg
     5581 catagcttcc ggctggtaac tcccagccat gataaagaag gtcccggtag ggtcaacgac
     5641 ccttgcccgg tagaacatgt tctgctcacc ctgtttcttc ttctcagtga gcgtaccgac
     5701 gaggaatacc ctgttgcacc gctcccctgt gggaaggagc acgtaggtag ggctcttctc
     5761 atcttccccc tcccggaagt gaatcctggt ctcacggagc tccccggcaa agacccgcct
     5821 ggcgggttca cgttcgaatg ttccctccct tcgggcgaac gaaggtctct caggggccgc
     5881 catcagagtg cccccccggc cctgttcagg agtgtcggga tctcaccggg ttcgaaatgc
     5941 tggaatgcac aggcagagac caggatcctt tggtcgatct ctcttcccct gcaggtgaag
     6001 tatcgcccga gaatacgctc ccgcatccgc aggaatacct catccatccc aagtggattg
     6061 ttctcggcaa gttctttcgc gtcattgatt gagatccctg tcagtttctc ggtaacttcg
     6121 cgttggacca gaatctcgcg agtctccttg ccattgtcga gccacccctt aatgcggaga
     6181 tcgtagagga atcctggctg gatctcatgt accgggcaat agttctgccg tgagaggacc
     6241 cggttgcatc cctcaaccgg acaacgcttt atgagcccgg atccaggtgc aatatggaca
     6301 agtgctcctg caaactcacc ttcacctcca ctcaccggga tatctccttc ttcaggaact
     6361 gttgttgcag tggtgatgtt gagtgagata cgcccctggt actcatcgat cagggcgtag
     6421 tagacgctat agacggttcc cgggttgagc cgttccttcc cctcctcctt ccatatcacg
     6481 aattttaccg taccggattc atcgccaaga agtccggact ggagcatccg atcatggctc
     6541 acatcccatt cctggataaa ttttcctctc acactgccaa taccaggggt cagttcagca
     6601 aggggggtta tttcagggat caggggcgtt tcacgggaga tctcttttat ggtggttccc
     6661 gaatggatct tcaggttggg tgttcctctg tactcatcca ccacgaccga ttcgaagcgg
     6721 taccatgagc cggcagacaa tcgtggggca tttgctttgg cccataccac aaaccgcatc
     6781 gcgccagtgg gatcggcgag gattcccgtc tgggatatcg cagcggaagg agaactggag
     6841 agagagacca cttttgcctc gatggttgcc cagtcccctg aagagagctg acgaagctgc
     6901 cgctcctccc ctgcggtccc tgccgtccct ggcaggaggt ccgggatgga gagatcacgc
     6961 gcaagctcgt tcaaaaccgt tcgttctgct tcctggggtg gtactccgaa ctcctccatg
     7021 aggcggcgga gttttgcctc gatttttgtc ctgtcggcct ggtggccttt tgcactgact
     7081 ttcagggaga ttctttcact tatactggaa taatccatgt tcatacatct ccagattaag
     7141 gtaggttgac cccacctaca taaagggtac gagcgggtag tgaaaatgat gcaccagctg
     7201 caggtttaag tatctgcacc ctcaaatata acagacatgg aattgacggc tgacagcacg
     7261 atctacgacc ttctgaaagc gaagcctgaa tcggccgagg tactcttcaa gttcgggatg
     7321 ggctgcgtgg gatgtgcaat tgcccgcggt gagacaatcc gcgaagcagc catggtccat
     7381 ggtattccgc tcgaagaact cacggctgca ctgggcatca aggaagcgtg aaagaaggtc
     7441 ggaaaacgga ggtctggcgt aatgtttcag gcctcattct gtttttctct ctgtctcatc
     7501 ttcagctcac gaagtgacgc ttcaggattc tgtttcaggt aatctgcaat tcctggctgg
     7561 tgaagcaggg tgcacccggc acagttccag actggtccat ttcttgatga gctcctgacc
     7621 cactggccaa gcgattcatc accgcatggg tagaaggggc aataacagaa ttcacagcgc
     7681 tgaccctcaa aatgacaggg ataatacggg caggcctttg gaagccactc aacccagtgg
     7741 tccccgccaa atcttgagaa gataaagtag gacgaagctt cccgtgtcac tactccttca
     7801 tgccgtcgca gggcttcggg tatcccgaac aggaccgctg catggatccg atgtccaacc
     7861 tcggtatgaa tccctgcgga gggatggaca accactcctt cagaggcaat gataaccgca
     7921 tccgtgggag tgccataaaa gggatggccg agtcctctga gggcctccga ctttgcttca
     7981 gttgccgtga tgattgcccc cgcgagtgct gcatcggaca tcccctggct gctgcagacg
     8041 atgatattga ttgtcccggg agattcctta tgaatccctg cagaaataaa aactgtcaca
     8101 aaatcatact ggaggatgca gaggtgctgc atcctgacag tggtgagaag accgaagaaa
     8161 tcggcgggaa gtccttcccg ggaggctaca agagcgagct cgcggagcgg ctcctgtcca
     8221 tcccagtcag gtcggagggt atggttgagc agcgtactga ccgtaccgat gccacccccg
     8281 actcctgtgc tgagcgaccg gtaactgccc cggacaaaga gggtattttc ccggagataa
     8341 tatctcatgc tacaccctta attcacgctt atttcaattt caatccttcc cttttcattt
     8401 gtgttacgtt tacggctcgg gcgcggtgag aagatgaatc agaatcaaaa taaacttgca
     8461 aatcccctct tctgggtttc aattccacct gggagtagta agaccgggga ggttgtattc
     8521 ggtgtgaaag caagtactga actgtttatg ctcatgcttc tcgatggaaa aggacaggtg
     8581 ttactaacag aacctgtcag accagttaca accggcccct atctgtcatc cgtggaagat
     8641 gctcttccca ataatacata tttctcttag cgaatgtgct tcacgagcct gtactcattt
     8701 ttttaaggta atggaaccat gctgttaaaa atcgaaccat aatcgccttt cccatcttgc
     8761 ggcgaattct cataacttct gaaatgatgg gactatgaac aagataaaaa acagctagtt
     8821 tcgatcagct acccaatcgg agcggcgtag aattgcttaa aggaggttgt gaaatatcgc
     8881 ttcgcgaaat acttcatgcg accgtgtatc gtttccggtc cttcagttcc tgctgtttac
     8941 cggcgttcca gcctgagact tcctggagat agccggtcac ccggcttatc tggacaacat
     9001 catgggaacc acagtcgggg cataccggct ccccgcagag gggacagtac tcgattcccg
     9061 atatgatctc atgggagcag tggcagtggt caagggggca catgat
//
LOCUS       DAOOCO010000062         9074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7254_length_9074_cov_5.586318, whole genome shotgun sequence.
ACCESSION   DAOOCO010000062 DAOOCO010000000
VERSION     DAOOCO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7254_length_9074_cov_5.586318"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..2767)
                     /locus_tag="PK154_04900"
     CDS             complement(233..2767)
                     /locus_tag="PK154_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HPW10433.1"
                     /translation="MTKRFSLALIALVALAMLILPASAAVNQIAQGGDVFIGEEGLDV
                     TAAVGNFTQIAWFASGTTPATDTPNYIVAVGNAASFYVAPADFVGRTGNWYNWNGANA
                     GVAFNVVDPNIDIKIWDQNANKDVTGKQVPAGNVENFRVETNTYSVINRPSYAGEGFV
                     NIKVKTADGATYTALETPANVMKTLTGQAITQSLWYWAVPAGNTSVGWDTAATDDAGS
                     RLYKAGTYTVSAELNLNKIKDNYKAPDGTDYSGKTVTALKTIAITGDTVKIEASKDTV
                     VRGNQFSVTVTGRANEFYYVWVKGTSSMTGGAGDQPPIMAASQDNVANDPVAGPYTIG
                     SYVYEGGAGKTIKQDVATGPDNGVYYYAKIKTSASGTRTVGFLTGANTDDKKYTIRVE
                     NNFGGQYKSDEVDVKVEKGTVTVVAAGDQSYFLGQEVKLSGTNSETDNVYLFITGPNL
                     PANGGLLTDPRSPVCGSCTPPVFTVTDVNDDNTWEYKWQTANLNIDAGTYTVYAVATQ
                     NDKSQLSDAQYGTVSVIIRKPFVTAQASQSTVAQGDDMYVRGVAEGDPSQGVAIWVMG
                     KNFVAYNTEAVNDDGTFEYEVDGGMTSGMTSGQYFVVVQHPMYNDELDVYPQANGGYT
                     YRYVVGPYPTANSVLFTLEGPGSLQGSDAANALTVALDNSMVDDTYTKLQFLIEVPEI
                     NILPVPEAMIGDKFEIKGTTNLAVDDELLVEVYSSSFGPTEKTQTGEFSGSSGTVKVV
                     KGTEGFNVWSFPVDTTTFKPDEYIASVSAIGLQSANDVTATTLFNVVEFVPTTVPTTV
                     PPTTVVNTTVPTTVPTTVPPTTTPGFGALVALIGLGAVAFLIVRKH"
     gene            3280..3993
                     /locus_tag="PK154_04905"
     CDS             3280..3993
                     /locus_tag="PK154_04905"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPW10434.1"
                     /translation="MRIIRAPSLGTAHDLVVKMVLEKGWVLNTEDREATIEFDEIAMR
                     IEHPDTEPMVSPHSRFSRKFVEQYAHDLLFGTSAVFEYDYHDRLYNWGTPLVAEGTAL
                     HIDQIAYIVRKLASSQVSRRAMAITWNPYIDEQLDDCPCLQLVQCLVRNGHLSMKVVF
                     RSNDMLTAAGANMFALVHLQKYIAEQLGLPLGYYTHISLVPHIYYKRDVNDIEPFCGK
                     GEYIKPATEVCRTCGKCRL"
     gene            4057..4129
                     /locus_tag="PK154_04910"
     tRNA            4057..4129
                     /locus_tag="PK154_04910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4091..4093,aa:Gln,seq:ctg)
     gene            complement(4474..5076)
                     /locus_tag="PK154_04915"
     CDS             complement(4474..5076)
                     /locus_tag="PK154_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HPW10435.1"
                     /translation="MIWIGDHLVSEGPGGVYGERTVQGYRLWSPKRSKLAALYHLGKG
                     IDLENYHRVLYLGAANGTTVSHVADYVEVVYAVEPAPRPMQDLMVVARQRKNIIPIMA
                     DARKPERYLPLVEKVDIIYQDVAQPDQAEILSVNSRFLVSDGIMVFMLKPRSIDVTRT
                     PAYVAEEVIHELSTKGIWTEECVWLSPYYPDHAALICKVS"
     gene            complement(5073..5927)
                     /locus_tag="PK154_04920"
     CDS             complement(5073..5927)
                     /locus_tag="PK154_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HPW10436.1"
                     /translation="MKSYWFLDREGGTCHALPHDQRILADRIKQLEGDKTAETPDWEY
                     AVKCGFAKNRGEYLDLLHATAMALTAERIDEVSKVDHPELILMVKMLDEIDIVINLLS
                     ERSVEWYRALNPEFSRKSEMPRGRKLRDLMRDGSDEALGEILDEIEQLTKRRSTLSGK
                     VAAKAAEHLPNCSALAGGLVAARLAAEAGGIRALALMPGSAIQVLGAKNALFCHLSTA
                     SPSPKHGLIYQYRGVHGSRKEKRGKVARVLAAKLAIAARIDYYRGEPDPVFITIAKEA
                     ILNAGRIQ"
     gene            complement(6032..7243)
                     /locus_tag="PK154_04925"
     CDS             complement(6032..7243)
                     /locus_tag="PK154_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPW10437.1"
                     /translation="MIGFLAGIVLGVLLGAISGLLPGIHVNALAGGLLALQLALIPVF
                     GPEMLAIAMVSALITHTFLDIIPSTFLGIPDADTALSVIPAHAMCLEGRGEEAVRIAA
                     LGSVAGFLASLPVALALLLLAPLVQGYLDWAVAIILTGVVGYLVLFSESPLHSGIIFL
                     ISGILGLFSFQFAFLGAGPFGSSGILMPLLSGLFGISLLLVSGKGPVPQQHFEGISLP
                     RRTVVKGTISGTIAGIVVGWLPGLSNATANSVVASVADYNRDRQGYLFATNAANTVNA
                     VAGLAAFYAIARTRNGVMVALSAIETPPFSVVLSAAACAAACAYLLTLLLASTAWRLG
                     GINRTGLTAGVIVFITLLSFLFCGLFGLFILLLATLLGLVPKLLNIPQVFCMGAVTLP
                     VILFSLGVSVF"
     gene            7319..8017
                     /locus_tag="PK154_04930"
     CDS             7319..8017
                     /locus_tag="PK154_04930"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPW10438.1"
                     /translation="MRIAITRLVGKEKDDASRCKSFGHECYPVSPLRAEIREDLISSF
                     VRSVEANEYDCLFFTSALPAKIIAPRLSRWPRVIAIGPRTALTLGHFGIDAETLPSFY
                     SRDFVPYLGSWIRGKRIGIPRADVPNPNLIEGIREAGGIPAEVRVYALIPTGDLLDLS
                     SAEAILFTSAMSFSTAVYDADIPVMKIAIGESTGAVMRGRGIIPDITGDGSLEGTLQE
                     LNRHLAAKNKPAGA"
     gene            8023..8949
                     /locus_tag="PK154_04935"
     CDS             8023..8949
                     /locus_tag="PK154_04935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HPW10439.1"
                     /translation="MAGRADMKQPFPFQDGLIVIDKPRGPSSHQVTAWVGEILQTRVG
                     HAGTLDPGVSGVLTVMIGKAVRLAPLLLSEEKEYVCLIRLHRDVAAPRIREILAEFTG
                     RIYQRPPRKSAVARNLRIRVVHEIDLLDAVDRYLLIRVRCDAGTYVRSLCHHIGLALG
                     SGAHMVELRRIRSGHFDETRACTLHDLEDAASAARQGNPEVLKSLILSPGAALISLPE
                     VVIRDTAVDAVCHGALLARPGLSRYDEFKKGETVVLTSIQGELVALGRTLISSQEAGL
                     LKNGLVISPSTVFMDPGTYPRGWKKRDHAISP"
BASE COUNT         2134 a         2406 c         2380 g         2154 t
ORIGIN      
        1 gctcccggat tgttcagcca atactataat catctgggag atgcccctat atgctctttt
       61 ttatgtcgga ttccagttct ccgtgccgga ttcccagtat tgaaaccgtc acccaacggg
      121 tagtgcaatg ctattcttcc tcattctggc agacccgggg gtatcgataa agaagccggg
      181 aaaagagtac ctatcaatcg gaaggatgca agaaaaaagg ggatttttgt gtttagtgct
      241 tgcgcacgat gaggaatgcc actgctccaa gcccgatcag cgcaacaagt gcaccgaatc
      301 cgggagtggt ggttggcggc acggtggtcg gcacagtggt cggcacggtt gtgttcacaa
      361 cagtggtcgg cggcacggtg gtcggcacgg tggtcggcac gaattcaact acgttgaaca
      421 gcgtggtggc agtcacatcg ttggcactct ggaggccgat ggccgagacc gaggcgatat
      481 actcatcggg cttgaaggta gtagtgtcaa ccgggaagga ccatacgttg aagccctcgg
      541 ttcccttgac aacctttacg gtacctgaag agccgctgaa ttcgccggtc tgggtctttt
      601 cagtcggtcc gaacgatgag gagtagacct caacgagcag ctcgtcatcg acagccaggt
      661 tggtggttcc cttgatctcg aacttgtctc caatcattgc ttcgggcacc gggagaatgt
      721 tgatctccgg cacttcgatc aggaactgga gcttcgtgta ggtatcgtca accatcgagt
      781 tgtcgagagc tacggtgagt gcgttcgccg catcagatcc ctgcaggctg cctggcccct
      841 caagggtgaa gaggacggag ttcgcggttg ggtagggtcc gacaacatag cggtatgtgt
      901 agccgccgtt tgcctgggga taaacatcaa gctcatcgtt gtacatcggg tgctgaacga
      961 cgacgaaata ctggccgctg gtcatgcctg atgtcatgcc accatcgact tcgtattcga
     1021 atgtgccgtc atcattcacg gcttcggtgt tataggcaac gaagttcttg cccatgaccc
     1081 agatagctac tccctgtgag gggtcgcctt ctgcaacacc acggacatac atatcgtcac
     1141 cctgggcaac ggtgctctgg gatgcctggg cagttacgaa cggcttcctg atgatgactg
     1201 aaacggtgcc gtactgggca tcggacagct gggacttgtc gttctgggtt gcgactgcat
     1261 agacggtgta ggttcctgca tcgatgttga ggttggctgt ctgccacttg tactcccagg
     1321 tgttgtcatc gttcacgtca gtgacggtga ataccggggg agtgcaggag ccacaaacag
     1381 gtgacctcgg gtcggtcaga agtccaccat tggcgggcag gttcggaccg gtgatgaaca
     1441 ggtagacgtt gtcagtctcg gagttggtac cggagagttt gacctcctgt ccgaggaagt
     1501 agctctggtc gccggcggca acaacagtta ccgttccctt ctcaaccttc acatcgacct
     1561 cgtctgactt gtactgacca ccgaagttgt tctcaacacg gatggtgtac ttcttgtcgt
     1621 cagtgttggc gcccgtcagg aatccgacgg tacgggttcc gctggctgat gtcttgatct
     1681 ttgcatagta atagacacca ttgtcaggtc cggttgcaac atcctgcttg atggtcttgc
     1741 ctgcgccacc ctcatagaca taggaaccga tggtatacgg tccggctacc gggtcgttcg
     1801 cgacattgtc ctggctcgct gccatgattg gcggctggtc tcccgcacca ccggtcatgg
     1861 agctcgttcc cttgacccac acgtagtaga actcgttagc cctgcccgtg acggtcacgg
     1921 agaactggtt tccgcgaaca acggtatcct tgctggcctc gatcttcacg gtgtcaccgg
     1981 tgatcgcaat cgtcttgagt gcagtcacgg tcttgccgct gtagtcagtg ccatcagggg
     2041 ccttgtagtt gtccttgatc ttgttgaggt tcagctctgc actaaccgtg taggtaccgg
     2101 ccttgtacag gcgggagcct gcatcatcgg tagctgcggt gtcccatcct acagaggtgt
     2161 tcccggcagg tactgcccag taccagagtg actgggtaat cgcctggcca gtcaaggtct
     2221 tcatgacgtt ggccggagtc tccaatgcgg tgtaggttgc accatcagcg gtcttcacct
     2281 tgatgttgac gaatccctca ccagcatagc tgggcctgtt tatcacagag taggtgttgg
     2341 tctcgaccct gaagttctca acattgcctg ccggaacctg ctttccagtg acatccttgt
     2401 tggcgttctg gtcccagatc ttgatgtcga tgttgggatc aacaacattg aatgcaacac
     2461 ctgcgttggc tccgttccag ttataccagt taccggtcct gccaacgaag tcagcaggag
     2521 caacatagaa gcttgcagca ttgcccacag ctactatgta gtttggtgtg tcggtggcgg
     2581 gagtggtccc tgatgcaaac catgcgatct gggtgaaatt ccctacggcg gcggttacat
     2641 ccaggccttc ttcaccaata aacacatctc cgccctgcgc aatctggttt accgctgctg
     2701 atgccggaag aatcagcatc gcaagggcta ccagagcaat caatgcaagg ctaaagcgct
     2761 tagtcatctt tgtcctcctt atactcatgt ccaatttgaa acgttcaatc caatgcacac
     2821 gtcccgaaca gttgtcggca atcgccgaaa accggttcat cgtgctccat actccgaata
     2881 acggaatatg attcaatatt tgtggaacgc ctaatatatc ttttttggtc gaacacagag
     2941 ggatatgcag gtctttgtgg gataaaacaa gcgatttgag taaaagcagg cattattttt
     3001 catttcctta tactattcat ccaatacgta cataaacgtg gtcattccac agttccgcct
     3061 atgtctcgcg catggtcgga gttcttcctg aaatcatatt aaatgaagga ttgaccccgt
     3121 gattttctta tccattccac ctcgtaatct ccagataccc tcctcccctt ttccccattc
     3181 aatggcaagt gaacggccgc cagtatcatt ctcacccttt cattagaacg gaatgtaatt
     3241 tccatatctt tacattccaa tattctgcat aaaggtagta tgagaattat aagggcgcct
     3301 tctcttggaa cagcccatga cctggtagta aaaatggtat tagagaaggg atgggtgctt
     3361 aataccgaag atcgagaggc gaccatcgag tttgatgaaa ttgcaatgcg gatagaacac
     3421 ccggatactg agccgatggt gagccctcac tcaagattct caagaaaatt cgtggaacag
     3481 tacgcccatg accttctttt cggtacttct gcagtcttcg agtatgatta tcatgatcga
     3541 ctctataact ggggaacacc cctggtggca gagggaacgg cacttcatat cgaccagatt
     3601 gcgtatatcg tccgcaaact tgcatcatcc caggtgagca ggagagcgat ggcaattaca
     3661 tggaatccct atattgatga acagctggat gactgcccct gcctccagct ggtccagtgt
     3721 ctcgtccgga acggacacct ttcaatgaaa gtggtattca ggagcaacga catgcttacc
     3781 gcggcagggg cgaatatgtt tgctctggta catcttcaaa aatatattgc agagcagctc
     3841 ggacttcccc tagggtacta tacccacatc tcccttgtac cccacatata ctataaacga
     3901 gatgtgaacg acatcgaacc cttctgcggg aaaggggaat atataaaacc agccacagaa
     3961 gtctgcagga cttgcggaaa atgcaggtta tgaaggttcc caggctcaaa tcagtcaggt
     4021 taattaggag aaagcaccaa taaaatagga tattacagcc cggtagtgta gcggtcaatc
     4081 atgcgggact ctggatcccg cgacagcagt tcgaatctgc tccgggctat taattatcct
     4141 ttttccaatg aaaatcactt cgtaattttc ataatttatg cccgtatccc gaaatcgggg
     4201 ctcataggag gtttatatga aaatgcatcc ggcattttca tcttttatgc ttgtggccct
     4261 caaaggaatc aggttatttt ctttaacgga tactgcagat ctgccatgat ctttcattat
     4321 cccatgtcct tccatatttg ggaccggttt gatgtggagt ttaacccccc ctcttgattt
     4381 aatcccctga tatctgaaaa gaggcttgta ctacctctgt agattcaaga aataaacaat
     4441 atttcagatg ttttacccac ttgatcgtca ttatcagctg actttgcaga tcagcgctgc
     4501 atgatcagga tagtaggggg agagccagac gcattcctcc gtccagatac ccttggtgct
     4561 cagttcatgg atcacctctt cagccacata ggctggggtc cgggtgacat caatgctcct
     4621 gggtttgagc atgaatacca tgatcccgtc tgacacaagg aacctgctat tgactgacag
     4681 tatctctgcc tggtctggct gggctacatc ctgatatatg atatcgactt tctccaccaa
     4741 ggggaggtat cgttcaggct ttctcgcatc cgccatgatt ggtatgatat ttttacgctg
     4801 ccgggctact accataagat cctgcatcgg cctgggtgct ggttcaactg catatacgac
     4861 ctccacgtaa tcggcaacat gggagacggt ggtcccgttc gcagcaccaa gatagagcac
     4921 cctgtgatag ttctcaagat ctatgccctt tcccaggtga tagagcgcag ccagcttgct
     4981 cctcttcggg gaccagagcc ggtacccctg aaccgtccgt tccccgtaca ctccaccagg
     5041 gccttctgat accaggtggt ctcctatcca gatcattgaa tcctcccggc attcagtatc
     5101 gcttcttttg ctattgtaat gaaaacggga tctggttctc cacggtagta atcgatccgt
     5161 gcggcaatgg caagttttgc agcaagaacc cttgcgacct ttcccctctt ctccttcctg
     5221 gatccatgca caccccggta ctgatagatc agaccgtgtt ttggagaggg tgaagcagtt
     5281 gagagatgac aaaaaagggc atttttggca ccgagcacct ggattgcact tcctggcatc
     5341 agagcaaggg cacgaattcc tcccgcctct gcagcaaggc gagcagccac cagtcccccg
     5401 gcgagggcac tacaattggg gaggtgttct gctgctttcg cagctacctt cccggacagg
     5461 gttgaccgtc gtttggtgag ctgctcgatc tcatccagaa tttcgcccag agcctcatca
     5521 gacccgtccc tcatcagatc tcgtagtttt cttccccgcg gcatctctga ctttcggctg
     5581 aattcaggat tgagggcccg ataccattcc accgatcgtt cagataatag attgatgacg
     5641 atatcgatct catcaagcat cttgaccatc agaattaatt cagggtgatc aacctttgac
     5701 acctcatcaa tgcgctctgc cgtgagggcc attgcagtgg catgtaacag gtcaagatac
     5761 tcaccacggt tttttgcaaa tccacatttc accgcgtatt cccagtccgg agtttcagcc
     5821 gttttgtccc cttcaagctg ctttatcctg tctgccagta tccgttgatc atgcggcagg
     5881 gcatgacatg tccccccctc ccggtcaaga aaccagtagg acttcataag cagtaatggg
     5941 tatgaaccct cattaggatt catccacagt tcaaggacgc aagggtgaga caggatgatg
     6001 atctggcccg gggccctgat tcccagcctc ctcagaatac ggagactccg agagagaaga
     6061 ggataaccgg gagggttacg gctcccatgc agaatacctg gggtatattg agcaatttcg
     6121 gaacaaggcc gagcagggtg gcgagaagga ggatgaagag cccgaacagg ccacagaaca
     6181 gaaaactcag cagggtgatg aacacgatga cacccgcggt aagcccggtc cggttgattc
     6241 cccccaatct ccatgccgtt gaagcgagga ggagggtgag gagataagcg caggctgctg
     6301 cacacgccgc ggcagagagc accacactga acgggggtgt ctcgattgct gagagcgcca
     6361 ccatgacacc attccgcgtc cgtgcaatag catagaatgc tgcgaggccg gcaacagcat
     6421 tcacggtatt tgcagcattc gtggcaaaca gatatccctg ccggtctctg ttataatcag
     6481 ctaccgaggc gactacagaa tttgcggtcg cattcgaaag accgggaagc catccaacca
     6541 caataccggc aatagtgcct gaaattgtcc cttttacaac cgtccggcgt gggagactga
     6601 taccctcgaa gtgctgttgc gggaccggcc ctttccccga tacaagaagg agggaaatgc
     6661 cgaacaagcc agaaaggagg ggcatgagta ttcctgaaga tccgaatggg cctgcaccca
     6721 agaacgcaaa ctggaaggag aacaacccca gtatcccgga tatgagaaaa ataataccac
     6781 tgtgcagggg ggactcagaa aaaaggacca gataccccac tacacccgtg agaatgatcg
     6841 ccacagccca atccaggtat ccctggacca acggcgccag cagaagcagg gcaagtgcta
     6901 ccggcagaga ggcaaggaac cctgccacgc ttcccaaggc agcaatacgg actgcttcct
     6961 cacccctgcc ctcgaggcac atcgcgtggg ctggaattac cgagagtgcg gtgtcagcat
     7021 caggaattcc aaggaatgtg ctggggatga tgtcgaggaa ggtgtgagtg ataagggctg
     7081 aaaccattgc gatggcaagc atctcaggcc cgaacactgg aatgagcgcc agctggagcg
     7141 ccagcaggcc cccggccagg gcattcacat ggatcccggg aagcaggcca ctgatcgctc
     7201 caagcaacac gccaagaaca attcctgcca ggaaaccgat catttccgcc taggttgggc
     7261 ggaatcaaga taaatacatc gctccaaaga tactggtagt aaaaggtaag tacgccaaat
     7321 gaggattgca atcacacgac tcgtcggaaa ggagaaggat gatgcatccc gctgtaagag
     7381 cttcgggcat gaatgctacc cggtatctcc attgagagct gagatcaggg aagatctgat
     7441 ctcttcgttt gtgagatcgg tagaggctaa cgaatatgac tgcctcttct tcacgagtgc
     7501 cctccctgca aagatcatcg ccccgcgact ctcacggtgg cccagggtga tcgccatcgg
     7561 accccggacg gcattgacgc tcgggcattt cggaattgat gccgaaaccc tccccagttt
     7621 ttattcccgt gactttgttc cctatcttgg ttcatggatc cgggggaaac ggataggaat
     7681 tccccgtgca gatgtgccga atccgaacct gattgaaggt attcgggagg cgggagggat
     7741 cccggcagag gtccgggtat atgcccttat tccaacggga gatctgttgg atctgagctc
     7801 tgcagaggca atcctcttca ccagcgcgat gtctttctcc accgcagtgt acgacgccga
     7861 tatcccggtt atgaagatcg caatcggtga gtcgaccggg gcagtgatgc gcggtcgggg
     7921 tatcatccct gacataaccg gggacggatc actggaagga acgcttcagg aactgaaccg
     7981 tcatcttgca gccaaaaata aacctgcagg agcctgaaga agatggcggg cagggccgac
     8041 atgaaacagc cttttccgtt ccaggatgga ctcattgtta tcgacaagcc caggggacct
     8101 tcaagccacc aggttactgc atgggtcggt gagattctcc aaacccgggt cgggcatgca
     8161 gggaccctcg accctggtgt ttccggtgtc cttacggtca tgattgggaa agccgttcga
     8221 cttgccccgt tactcctctc agaagagaag gaatatgtct gtctcattcg gctccatcgt
     8281 gatgtcgctg ccccccgcat ccgggagatc cttgcggaat tcaccggccg gatatatcag
     8341 cgacccccac ggaaaagtgc agtggcaagg aacctccgga tccgcgttgt ccatgagatc
     8401 gatcttctgg atgctgttga ccggtacctg ctgatccgtg tcaggtgcga tgcagggacc
     8461 tatgtccgct cgctgtgcca tcatataggc ctcgctcttg gctcaggggc acacatggtt
     8521 gaactcaggc ggatacgttc aggtcacttt gatgagacca gggcctgcac cctccatgac
     8581 cttgaagatg cggcatcggc cgcacggcag ggaaaccctg aagtgctgaa atcattgatc
     8641 ctctctcccg gtgccgcttt aatctccctt ccagaggttg tcatccgtga tactgcggtt
     8701 gatgcagtct gtcatggtgc cttacttgcc cgtcccggat tgagcaggta tgatgaattc
     8761 aaaaaggggg agaccgtcgt tctcacgagt atccaagggg aacttgtcgc cctcggccgg
     8821 acactcatct catcccaaga agcaggacta ctcaaaaacg ggctggtcat ctccccctca
     8881 acggtattta tggatccagg aacatacccc agaggctgga aaaagcggga ccatgctata
     8941 tctccatgag atagcattaa aaaaaatccc ctacaatagt aagggtgcat ttctgtgctg
     9001 aggtagtcta gaccggtagg gcgcaggcct ggaaagcctg tggggcgtcc agcccctcgg
     9061 gagttcaaat ctcc
//
LOCUS       DAOOCO010000063         8984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7331_length_8984_cov_5.291410, whole genome shotgun sequence.
ACCESSION   DAOOCO010000063 DAOOCO010000000
VERSION     DAOOCO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7331_length_8984_cov_5.291410"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(457..1299)
                     /locus_tag="PK154_04940"
     CDS             complement(457..1299)
                     /locus_tag="PK154_04940"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPW10440.1"
                     /translation="MVTIGVHVSIAGGISKAVGRASATGCDTFQIFSRNPRGWMFRDL
                     EPAEVILFRHELGKSKLAPAVDHMPYLPNLATMNPEFAEKSGSTLVAELSRCGTLGIP
                     YLVTHLGHYGPEGKEAGQRRVSTLINEALDKVDNPVLLLLENTAGEKNGIGSRFEDIA
                     TVMEGIKKPSRIGFCFDTCHAFGAGYDLRSGEGLEETLNRFDETLGLKHLRLIHLNDS
                     KGDLGSGLDRHEHIGLGRIGEEGFARILSTPHLRNLPLICETPVDERRDDAGNIKKVR
                     ELAG"
     gene            complement(1618..2589)
                     /locus_tag="PK154_04945"
     CDS             complement(1618..2589)
                     /locus_tag="PK154_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPW10441.1"
                     /translation="MRVAIIEGDGIGHEVIPPAREAIHIIRPDIEFFEVEAGFDRWQR
                     SGEAITEDDIALLATADSILFGAVTTPPSRDYRSVIVRIRKELDLYANVRPVWGEGFD
                     IIVVRENTEGLYSGVEKIEHDSACTLRCITRKGSERIARYACTLARRRRDITIGHKAN
                     ILKSDVFFRDICLEEARKAAIPVKERFIDALCLDVLQHPDRYDVIVTTNMFGDILSDV
                     TAYLVGGLGLLPSGNIGTDHSLFEPVHGSAPDIAGRNIANPVAAFRSAAMLLHHIGEH
                     ASCGLVEQAIRATIARGIKTRDLGGTAGTSEFARAVLAELEAMVPRC"
     gene            complement(2586..3389)
                     /locus_tag="PK154_04950"
     CDS             complement(2586..3389)
                     /locus_tag="PK154_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10442.1"
                     /translation="MIFPRQCKEVGIASTRPCGDQVYFLSRYLIHDTPAGPEILEVTT
                     DPSGKGLMREVMETRVLAGPGETWLYPDKVQLHNRALLVRLASESGKRCTIFTGIDEH
                     TTFVCDPDLSIFQTVAVYDVIPPRPSLSACIRDLEEAGLFSGLDVTFEHTLRDISQVD
                     ADIYPCRASGFDRTLDADLLLGGEKVAGCLTATQFLRECYGEGFDVTDICPLSMVAEE
                     PFIARCCRKEREGIGTHGGKFGAVVHWGASPSEIFSAVSDLLATWRAGR"
     gene            complement(3386..3856)
                     /locus_tag="PK154_04955"
     CDS             complement(3386..3856)
                     /locus_tag="PK154_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPW10443.1"
                     /translation="MKIEGHAVCLPADIDTDQIIAGRYLRTKDRSIWKRHVFEDLDPD
                     LAERLEGAVIVAGKNFGCGSSREQAPVALKEAGVRAIVAPSFARIFFRNAINIGLPLA
                     EAEIPCSDGDMVEIDLTAGLITVSGISRDIRPLSPRMLQMLAAGGLVSHWRQKR"
     gene            complement(3853..5067)
                     /locus_tag="PK154_04960"
     CDS             complement(3853..5067)
                     /locus_tag="PK154_04960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPW10444.1"
                     /translation="MPTLSERILTGGEGEYVDREVDRAYVHDGTGVLTYETFRALGVT
                     EVSHPSRAYVLFDHIVPANNSTTADLQHELRNFASSSRLQFSDIGGGICHQLMGEGIA
                     LPGEIIAGADSHTCTLGAFGAFATGVGATDMAAIFASGMTWFRIPATIGLFLSGSLSG
                     GAEAKDLALTYVARLGMDGATYHALEFIGDGVSSLSMDSRMTICNLSVETGAKTGMFY
                     TDEVTRAYLQSYGHETAVQDPPDCAYSQEYTFDLKAIEPVIAVPPRVDTVRPVAEMAG
                     IPLDQVFVGTCTNGRYEDLSRFATIVRGKKVKIRTIIGPASRNVLRKAAETGVLSDLI
                     TAGCTVLPPGCGPCLGAHMGVIGEGEVCLSTSNRNFRNRMGVGGEIYLSSVATAAASA
                     LKGEIVVPGGVV"
     gene            complement(5071..6195)
                     /locus_tag="PK154_04965"
     CDS             complement(5071..6195)
                     /locus_tag="PK154_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HPW10445.1"
                     /translation="MKPWNIEICDVTLRDGEQTPGVSFSCEEKQAIASKLDEIGVEVI
                     EAGFPVVSPYEKQCVKTVAGMGLDARICCLARARKGDVEAAVDCNVDMVSIFIATSDR
                     HIRHKYHKPREAVLEEALQMVDFARDHGLAVRFAAEDASRTDMEFLLEVYRGGADHGA
                     DLLSFADTVGSLVPMQMFRIMSTLVSTVDRPFCAHCHNDMGCATANTVTAAEAGAFQL
                     HTTVNGIGERAGNAALEEVLVALRMNGGVDRYDLSHLCDISTMVQAYSGISLQKTKAV
                     VGEHAFSHESGIHIAAILEDPQTYEYFLPDMVGGERRFILGKHTGKKALEHVVATLGY
                     SLSEQQICKVLDEVKELGESKCGITPETLTALIRHVRKEQ"
     gene            complement(6176..7447)
                     /locus_tag="PK154_04970"
     CDS             complement(6176..7447)
                     /locus_tag="PK154_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW10446.1"
                     /translation="MKGFEVLASALSRSADHCYAVPGYPATELSAMAKAEPVIAEKVA
                     LEYALGDSIMGKRAAVIMKNVGLNNGADLLVQATTQGLVGGVVIVAGDDPMAAGSTNA
                     MDSRYFGELAQTPVLEPDPEVCSASVEEAFSVSESFSRIAILRVTPQLVFGEAVATAV
                     PRTPGEGKVADPRLTMKGRVDHADSYLPGMFAWSARSRLNRMKGGVAGVGAAEGDTRI
                     VTVYPPPPGLAGFTRINEIGRPFVAEHLAVVPPAAIPPPQTSESRGFYRTFCRNCPFI
                     PVFDIMKRNGIQAIADAGCSILALNPPYCIASASYGLGSAIGVAARSTHVALIGDYAL
                     LHSGLQSLIDVYEKRLSLLCIVLENRCMGMTGGQEAPSPCKYIRWADPLAVRASAIKQ
                     LEELITIPEEPVTLVVQGTCPEGRHHETVEY"
     gene            complement(7447..8418)
                     /locus_tag="PK154_04975"
     CDS             complement(7447..8418)
                     /locus_tag="PK154_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10447.1"
                     /translation="MEWPELKARLLAEGTTRLTGKSALRYRSSSTAGPGAGTGGSVFF
                     SCGKGRVRLAIDQNSPLEIVHLGSGRAILKAWHLDIRGNLEPIALHCPRQAYITVTGG
                     CIYRCKYCEVPAIDAGRKTPEEIEAMVEQVQDQSDSIALTSGVLQSVDEEEEYVLGVI
                     GRLRRFALPIGVSIFPGKGTAERLHAAGVAEVKFNVEAATEQLFTKMCPGLSYRSVWD
                     ALEDSVPLFGRNHVFSNLIIGLGETDREAECCIGNLCELGVIPVLRPLNPAGELSGFS
                     RPSAERLLRLFAFHTEALSAAGLDPCRALTMCIECTGCDLVPGRDRA"
     gene            complement(8400..>8984)
                     /locus_tag="PK154_04980"
     CDS             complement(8400..>8984)
                     /locus_tag="PK154_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10448.1"
                     /translation="GVGASAGRSYAGGVIRHQTDPSGGGRKAGATIWLPRMQRVLIGV
                     DDTDTPDQGASWTLAHNIARAVEDETARYLSHTIVQLFPVPYRTKNCVSIVCEFASAN
                     PGRLVARFHNLLRKYTLSDETGMAAFTGFDPSPLLPFAWMVKKGEVRRDLLPEIDGGH
                     LELIMEGRGIIGAVAAIPFYTRFEEALSLWNGRS"
BASE COUNT         1965 a         2688 c         2430 g         1901 t
ORIGIN      
        1 gatcgcctcc cttttccacg aagatgacca gatactgccc agaaaatttg cccccttaag
       61 gcccgtgaat cccgattcca ccacatattt tcccgtattt taaaaaacag ggaatcggcc
      121 tcgttttctg atatacacca tatagcgcta tatcttatgc cggagccgat gaatggagag
      181 agcgagcaga ctccggtttc cctgtgagct ccgggaggaa tggaagacgt caaaatcaaa
      241 ggttcccccc ggaaaaagtg cagcccggga acttgtctcc tgttaaaacc gggtctccca
      301 ttaaggacct gtcttgctct ggaggagtga aacgaggagg gaacaaaaga tacgttgaag
      361 aaagggagaa acggctttca gatcgagggg gaacagtttt tcctatccga cagatcttcc
      421 tcattccgcc agggcaacag cagaatcacc cattcctcac ccggcaagct cacggacctt
      481 tttgatattc cccgcgtcat cccttctctc atcaaccggg gtctcgcaga tcagggggag
      541 atttcgcagg tggggagtgg agaggatccg tgcaaatccc tcttcaccga tccggcccag
      601 accgatatgt tcatggcggt cgagccccga gccgagatcg cccttcgaat cgttcaggtg
      661 aatgagccgc aggtgtttca gaccgagtgt ctcatcaaac cggttcaggg tctcctcaag
      721 tccttcacct gaacgaaggt cgtatcctgc cccgaatgca tggcaggtat caaagcagaa
      781 gccaatccgg gaaggttttt tgataccttc catgacggta gcgatatctt cgaaccggct
      841 cccgattccg ttcttctcgc cggctgtatt ttcgaggagg agaagcaccg gattatctac
      901 cttgtccagg gcttcgttga tcagggtgga aacccgcctc tgcccggcct ccttcccttc
      961 cggcccatag tgtccaaggt gggtcaccag ataggggatg cccagcgtcc cgcaccgtga
     1021 gagctcggca accagcgtgc tccctgattt ttccgcaaat tccggattca tggtggccag
     1081 gttgggaagg taaggcatgt ggtcaacggc aggggctaat ttgcttttcc cgagctcgtg
     1141 acggaaaagg atcacttcgg caggttcgag atccctgaac atccagcctc ttggattcct
     1201 cgaaaatatc tggaaggtat cacaccctgt ggctgatgcc cgtcccacgg cctttgagat
     1261 acccccggcg attgacacat gtactcctat cgttaccatc aaccaatctc cttctatcac
     1321 gtagggtagg gattgtggat acttcgtttt tgggttcctt ccttcccggt tcctggtctc
     1381 aatgaagcac acctccatcc aggcacgtca ggggagagag gataagatat gggatgggga
     1441 gatgaaagac cagccccacc acgggcgtta taccttcagg ttcttctcct caatattccg
     1501 gatggaaaga atatgcaggg gcgagaatgc ctatcgatat atcccgggaa gaggagcaga
     1561 tgactacaca cagatccagt ccggccaggc cgggaggggg aagcattact cgctttttca
     1621 gcaccgtggt accatggcct cgagttctgc aaggacggcc ctggcaaatt cagaggtccc
     1681 tgcagttcct cccaggtccc tggtctttat tccccgggca attgtcgccc tgatagcctg
     1741 ctctacgagc ccgcaggatg catgctctcc gatatggtga aggagcatgg ctgcactcct
     1801 gaatgctgcg acagggtttg caatatttct tccggcaatg tccggtgcac tcccgtgcac
     1861 cggttcgaaa agggagtgat cggtaccgat atttccgctt ggcaggagac cgagcccccc
     1921 taccagatat gcggtcacat ctgacagaat atccccgaac atgttggtgg tgacgatcac
     1981 atcgtagcga tccggatgct gaagcacatc gaggcaaagc gcgtcaatga agcgttcctt
     2041 taccgggatt gcggctttcc gggcttcctc aaggcagatg tcccggaaga atacatccga
     2101 tttgagaatg ttcgctttgt gaccgatcgt gatatcccga cgtctcctgg caagggtgca
     2161 ggcataccgg gctatccgtt cacttccttt ccttgtgatg cagcggagtg tacatgccga
     2221 gtcatgctct atcttttcaa caccggaata cagtccctcg gtattctccc tgacaacgat
     2281 gatgtcaaaa ccctcacccc agacaggcct gacattggca tagagatcaa gctctttccg
     2341 gatccggaca atgacgctcc ggtaatcacg ggagggaggg gtggtaaccg ccccaaacag
     2401 gatcgaatcg gcagtggcaa gcagcgcgat atcatcctcc gttattgcct cgccgctccg
     2461 ctgccacctg tcaaacccgg cctccacctc aaaaaattca atgtccggcc ggatgatatg
     2521 aatcgcctca cgagccggcg gtatgacctc atgaccaatc ccatctcctt cgatgatggc
     2581 aaccctcatc ttcccgccct ccaagtggcg aggagatcgc tgactgccga gaagatttca
     2641 gagggagagg ctccccaatg gaccacggcc ccgaacttcc ccccatgcgt cccgattcct
     2701 tctctctctt tccggcagca tctcgcgatg aatggttctt cagcgaccat tgaaaggggg
     2761 cagatatccg tcacgtcgaa cccttcacca taacactccc gaagaaactg ggtggcagtc
     2821 aggcacccgg ccaccttctc tcctcccagg aggaggtcgg catcaagggt ccggtcaaac
     2881 cctgatgccc tgcagggata gatgtcagca tccacctgcg agatatcccg gagggtgtgc
     2941 tcaaaggtta catcgagccc ggagaagaga cccgcctctt caagatccct gatacaggct
     3001 gaaaggcttg gacggggggg aatgacatca tacacggcca cggtctggaa gatcgagagg
     3061 tcaggatcac agacaaacgt ggtatgttca tcaatcccgg tgaagatggt gcaccgcttt
     3121 cctgattctg aggcgaggcg cacgagcagg gctcggttat gcagctgtac cttgtccggg
     3181 tagagccagg tctcaccagg gcctgcaaga acccgggtct ccatgacctc ccgcatcagg
     3241 cctttgcctg acgggtctgt tgttacttcg aggatttccg ggccggcagg cgtatcatgg
     3301 atgaggtagc gggagaggaa gtacacctga tccccgcagg ggcgggttga tgcaatcccc
     3361 acctccttgc actggcgggg gaagatcatc gcttctgcct ccagtgcgat acgaggcccc
     3421 cggctgcaag catctggagc atccgggggg agaggggccg gatatcccgg gagatcccgg
     3481 atacggtgat cagccctgcc gtgagatcga tctcaaccat atccccgtca ctgcatggta
     3541 tctccgcctc agcaagggga agcccgatat tgatggcatt cctgaagaag atccgtgcaa
     3601 aggaaggggc gacgatggcg cggacccctg cctccttcag tgctaccggg gcctgctcac
     3661 gggaggagcc gcacccgaaa ttcttgccgg caacaatcac cgccccctca agcctttcag
     3721 caagatccgg atcgagatcc tcgaagacat gacgtttcca gatggaacga tccttcgtcc
     3781 ggagataccg tccggcaatg atctggtccg tgtcgatatc tgccggaaga cagaccgcgt
     3841 gcccttcgat cttcatacca cccctccggg gacgacaatc tcccctttca gggctgatgc
     3901 tgcagcggtt gccaccgaag agagatagat ctcccctccc acccccatcc tgttcctgaa
     3961 attccggttc gaggtcgaga ggcacacttc tccctctcca atcactccca tatgagcccc
     4021 aaggcaggga ccgcatcctg gcgggagcac cgtgcagcct gctgtgatga ggtctgacaa
     4081 aacgccggtt tcggctgcct tgcgaaggac gttccttgat gcagggccga tgatggtccg
     4141 tatcttcacc tttttgcccc ggactatcgt ggcaaagcgg gagagatcct cataccgtcc
     4201 gttggtgcag gtcccgacaa aaacctggtc gagggggatc cctgccatct cagcgacggg
     4261 cctgacggta tccacgcggg gggggacggc aatcaccggt tcaatcgctt tgagatcgaa
     4321 cgtatactcc tgggagtatg cgcagtctgg tggatcctgc accgcggttt cgtggccgta
     4381 cgactggagg tacgcacggg tcacttcatc ggtgtagaac atcccggttt ttgcccctgt
     4441 ttcaacagaa aggttgcaga tcgtcatgcg ggagtccata gagagggagg atacgccatc
     4501 tccgatgaat tcgagggcat ggtaggtggc cccatccatg ccgagccggg caacgtacgt
     4561 gagggcaaga tcttttgctt cggctccgcc ggagagagag cctgaaagga acagcccaat
     4621 cgtagctggt atcctgaacc aggtcatccc ggatgcgaag atcgccgcca tgtcagtagc
     4681 ccccacgccc gtcgcaaatg ccccaaaagc tccgagggtg caggtatgcg aatcggcgcc
     4741 ggcaatgatc tcgccgggga gtgcgattcc ctctcccatg agctgatggc agatcccgcc
     4801 accgatatca gagaactgga gtcgcgaaga gctggcaaag ttccgcagtt cgtgctggag
     4861 atctgcagtg gtggaattgt ttgccggcac gatatggtca aagaggacat atgcccggga
     4921 aggatggctc acttcagtga ccccaagtgc ccggaacgtc tcataggtca atactcccgt
     4981 gccatcatgc acataggcac gatccacctc gcgatcaaca tactccccct cgcctcctgt
     5041 gaggatccgc tctgacaggg ttggcatcgt ttactgctcc ttccttacat gccgtatcag
     5101 ggcagttagg gtctcagggg taataccaca cttactctcc ccgagttcct tcacttcatc
     5161 gagtactttg cagatctgtt gttccgagag cgagtatccg agggttgcca cgacatgctc
     5221 gagcgccttt ttgccggtgt gcttcccgag gatgaaacgc cgttctcccc cgaccatgtc
     5281 aggcaggaag tactcatagg tctggggatc ttcgagtatc gcggcgatat ggataccact
     5341 ttcatgagag aaggcatgct ccccgaccac cgctttcgtc ttctgaaggg aaatgcctga
     5401 ataggcctgg accatcgttg agatatcgca cagatgggag agatcatacc ggtccacacc
     5461 cccgttcatg cggagcgcca ccagcacctc ttcaagcgcg gcattccctg cccgctcccc
     5521 gatcccgttg accgtggtat ggagctgaaa agcccccgct tctgcagccg tgaccgtatt
     5581 ggctgtagca caacccatgt cattgtgaca gtgggcacaa aaaggccggt cgaccgtgga
     5641 gaccagcgtg ctcatgatcc tgaacatctg catggggacc aggctgccta cggtatcagc
     5701 aaaactgaga aggtcagcac catgatcagc accgccccgg tacacttcaa ggaggaactc
     5761 catgtcggtg cgtgaagcat cctcagccgc gaaacgaacg gcgagcccat ggtccctggc
     5821 aaaatcaacc atctggagcg cctcttcgag cacggcctcc ctcggcttat ggtacttgtg
     5881 ccgtatatgc cgatcagagg tggctatgaa gatgctcacc atgtccacat tacagtcaac
     5941 tgccgcctcg acatcccctt tacgcgcccg tgccaggcaa cagatccggg catcaagccc
     6001 catccctgca accgtcttta cacactgttt ctcatagggc gaaacaacgg ggaagccggc
     6061 ttcgatcact tccaccccga tctcatcgag ttttgaggcg atcgcctgtt tctcctcgca
     6121 ggagaatgaa acaccagggg tctgttcccc gtccctgagt gtcacatcac agatctcaat
     6181 attccacggt ttcatggtgt ctcccttccg ggcaggttcc ctgaacgacg agagtaacgg
     6241 gctcttccgg aatggtaatc aattcttcga gttgttttat agccgatgcc ctgactgcca
     6301 ggggatctgc ccaccggatg tatttgcagg gggagggagc ctcctgccca ccggtcattc
     6361 ccatgcaccg gttctcgagc acgatgcaga ggagggagag tcgcttttca tacacatcga
     6421 tgaggctctg gagccccgaa tgcaggagtg cgtagtcgcc tatcagggca acatgcgtgc
     6481 tccgggcagc aaccccgatg gcactcccaa gaccatagct ggcgcttgcg atacagtaag
     6541 ggggattcag cgccaggatt gaacatcctg catcagcgat cgcctgtatc ccgttcctct
     6601 tcatgatgtc aaacaccgga ataaacgggc agtttcgaca aaatgtccgg taaaagcccc
     6661 tgctctccga tgtttgtggc ggagggattg ctgcaggagg aacaaccgcc agatgttcgg
     6721 caacaaacgg tctgccgatc tcatttatcc gggtgaatcc ggccaggccg ggagggggag
     6781 ggtagacggt cacaatccgc gtatcgcctt cggcagctcc gacccctgca acaccgcctt
     6841 tcatccggtt cagccggctc ctcgctgacc atgcaaacat ccctgggaga tacgagtccg
     6901 catgatccac ccttcctttc atggtaaggc ggggatcggc aaccttccct tctccgggcg
     6961 tccgcggaac agcagtcgcc acggcctcgc caaacaccag ttggggagta acccggagga
     7021 tggcgatccg ggagaatgat tctgaaactg agaatgcttc ctcaacactt gcagaacaga
     7081 cctccggatc aggctcgagg acgggggtct gtgccagctc gccaaaataa cgggagtcca
     7141 tcgcgttcgt ggaacctgct gccatgggat cgtccccggc aacgataacc actcccccga
     7201 ctaatccctg ggttgttgcc tggacaagga gatcggcacc gttgttcagg cctacgttct
     7261 tcatgatcac ggccgccctc ttccccatga tcgagtcgcc gagagcatac tcgagtgcga
     7321 ccttctctgc aatcaccggt tcggcctttg ccatcgctga aagttccgtg gccggatatc
     7381 ccgggaccgc atagcagtga tcagcactcc gggaaagggc agaggccagc acctcaaatc
     7441 ctttcattac gctctgtccc tccccggcac caggtcacac ccggtacatt cgatacacat
     7501 ggtgagagca cgacagggat caagccctgc agcggagagt gcctcggtat ggaacgcaaa
     7561 gaggcggagg agccgttcag cagagggtcg gctgaaccct gataactccc cggctggatt
     7621 gagcggccgg aggacaggga tgaccccgag ttcacagagg tttccgatac agcactctgc
     7681 ctctctgtcg gtctcaccaa gtccgatgat aaggttggag aatacatggt tcctcccaaa
     7741 caaaggtacg gaatcctcaa gggcgtccca tacggaacgg taagagagtc ccgggcacat
     7801 cttcgtgaat aactgctccg ttgccgcctc gacattgaat ttcacttcag ccaccccggc
     7861 agcatgcagc cgctctgcag tacctttgcc gggaaagatt gagactccaa tcgggagagc
     7921 gaaccgccgc agtctcccga tcacccccag gacatactcc tcttcctcgt cgacgctctg
     7981 aagcaccccg ctggtaaggg cgatcgaatc actctggtcc tgcacttgtt ccaccatcgc
     8041 ttcaatctct tcaggggtct ttcttcctgc atcgattgcc gggacctcgc agtatttgca
     8101 gcggtatata cacccccccg tgacggtgat atacgcctgc ctgggacagt ggagcgcaat
     8161 aggctcgaga ttccccctta tatcaagatg ccaggctttc aggatggctc tcccggaccc
     8221 aagatgaacg atctcgagcg ggctgttctg atcaatagcg agcctgaccc tgcctttacc
     8281 gcaagagaaa aacaccgaac ccccggttcc cgcacccgga ccggcagtgg atgatgaacg
     8341 gtaccgcaat gctgattttc ccgtgagcct ggtggtccct tccgccagga gccgtgcttt
     8401 cagctccggc cattccataa cgagagcgcc tcctcaaaac gggtgtagaa cggtatcgca
     8461 gcgaccgcac caatgattcc cctcccttcc atgatcagtt ccagatgccc tccgtcgatc
     8521 tcgggaagga gatcgcgccg aacctctccc ttcttcacca tccaggcaaa gggaagaaga
     8581 ggggagggat caaacccggt gaatgccgcc atccctgttt catcagagag cgtatacttc
     8641 cggaggaggt tgtggaacct ggcgaccagc ctgccaggat tggcagaggc aaattcgcag
     8701 acgatactga cacaattttt ggtccggtac ggaacgggga agagctgcac tatggtatgc
     8761 gagaggtacc gtgccgtttc atcttcaact gcccgcgcaa tattatgggc aagcgtccag
     8821 cttgccccct gatccggggt atcggtatcg tcaactccaa tcaggaccct ctgcatcctg
     8881 gggagccaga tcgttgcacc tgcctttctg ccccctcccg aaggatcggt ctggtggcgt
     8941 atgacacccc ctgcatagga tcggccggca gaagcgccca cccc
//
LOCUS       DAOOCO010000064         8975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7342_length_8975_cov_5.432511, whole genome shotgun sequence.
ACCESSION   DAOOCO010000064 DAOOCO010000000
VERSION     DAOOCO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_8975_cov_5.432511"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="cbiQ"
                     /locus_tag="PK154_04985"
     CDS             complement(<1..659)
                     /gene="cbiQ"
                     /locus_tag="PK154_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPW10449.1"
                     /translation="MIEDLFFLEKQAYKKSPVHALDARVKIIIAFAAIIAIVAVPYST
                     MIYTVGAIFFAFFVVLWTVSRLSPAVYLKRLAVILPFGIFLIVFQIFFKNPYYESFTT
                     IATLPFGIEIYAESVQFASILLVKFLVSVSFIILLSSTTRTQDLLEGAGRLGLPAEFT
                     LTLGMMLRYLYVFGYMIRKMTQSLETRCFNPFDRTLPYRYRLEKVAYAVGTLFIRSYE
                     QG"
     gene            complement(656..1489)
                     /locus_tag="PK154_04990"
     CDS             complement(656..1489)
                     /locus_tag="PK154_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10450.1"
                     /translation="MHLLETRDLTYVYPGNVTGLEHVNFIAGRNARIAVIGANGAGKS
                     TLFKHFNGILRPTSGTVLVHGEPITQKNIREVRKFVGLVFQNADDQIFSPTVEQDVAF
                     GPMNLGLDAETVNHRVDEALAMVGIEHLRERVPHHLSGGEKKRVAIAGVIAMEPQVIV
                     LDEPTAGLDPKGVKDLVGVINALAREYGITVIFSTHDVSLVPEVADHLYVMHRGSIVA
                     EGRVSDVFADLELLSSVRLDVPVIPKLIRQLREEGIEIAMAYTLDEAKAAFLKTYRER
                     A"
     gene            complement(1532..1882)
                     /locus_tag="PK154_04995"
     CDS             complement(1532..1882)
                     /locus_tag="PK154_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HPW10451.1"
                     /translation="MMDKKIFILAGIAIALIIAAMAPFIASSNPDGLESAFFGIFGAK
                     DLRGSTLDEEMAGTAEEEVTAITGNEYSFESPFPDYSIEGLAKFGEAAAVIIGTLVVV
                     GIAFGLSRVVARKG"
     gene            complement(1879..2517)
                     /gene="cbiM"
                     /locus_tag="PK154_05000"
     CDS             complement(1879..2517)
                     /gene="cbiM"
                     /locus_tag="PK154_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HPW10452.1"
                     /translation="MHIPDAFIPLPQAAVYWIIALIFIALALRWARRELSEEKIPLVA
                     ILAAGIFAIQAVNIPIPWGTSGHMMGAALAAIVLGSPFAAVFILTLVLIVQGVLFGDG
                     GLTVMGANIINMGVIGGFVGYYSYRGLLPVFKSPYAPAFIAGWLSLFVAALACAVEMA
                     VAGTFPLELGLFSMGLYHAVIGIIEGGITAFAIYLIATARPDIMADTTVVPA"
     gene            complement(2577..3740)
                     /locus_tag="PK154_05005"
     CDS             complement(2577..3740)
                     /locus_tag="PK154_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW10453.1"
                     /translation="MTERDQYDVVVVGAGPAGSTCARRCAELGLSTCLIEEHAAIGYP
                     VQCAGLLSARALAACQVSEKPVLHEVSGASIVTSRGSRLTFDAGTTKAFVVDRGLLDR
                     EMATAAADAGAEVRVKTAYLKRSGSSIITRGVDGKGEIACRMLIAADGPRSPVARDLG
                     MERPPVFLAGIQAEVPREMGGTLVEIYPDASPDFFGWIIPSGKGRARIGLAGRREVAS
                     RFSRFISQQGVRSCCHLVTGTIPIGVMPRTYGHGTLFIGDAAGFPKPTSGGGVYTGVR
                     SAYHAAETTCEACNEGSAADNLLCRYEQRWRAELGRELDLGFRLFSARGKLTGEEVDQ
                     LIRVLNDPSLKADILMYGDMDQPGVLLRRILKNPKIYPVLGILFRSGVRQFIK"
     gene            complement(3804..3968)
                     /locus_tag="PK154_05010"
     CDS             complement(3804..3968)
                     /locus_tag="PK154_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10454.1"
                     /translation="MAMEQGTEEAAIPSESFVMECPGTCSRISTFAGSPEHHTKTGSG
                     YPRQDCMGRG"
     gene            4005..4367
                     /gene="pth2"
                     /locus_tag="PK154_05015"
     CDS             4005..4367
                     /gene="pth2"
                     /locus_tag="PK154_05015"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HPW10455.1"
                     /translation="MEKGPVFEWKQCLVVRSDLRMSCGKMCAQAAHASILACGRSKAA
                     SRRRWCAEGQKKVVLKVAGEKILFELKTLAEVAGIAAALVQDAGMTEVPPGTITALGL
                     GPAKSEDLDKITGDLALL"
     gene            4364..5626
                     /gene="truD"
                     /locus_tag="PK154_05020"
     CDS             4364..5626
                     /gene="truD"
                     /locus_tag="PK154_05020"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPW10456.1"
                     /translation="MRESPYPLEQLLGMRYYGTDTPGTGGRLRALPEDFIVEEIPGDI
                     GTDGRFLICRLTKKDWDQQRAIKEIAKRLGISYKRIGFAGTKDKHAVTSQLISIEGIT
                     AEEIEQVRIRDITLEPVGRSSSPLALGSHAANRFTITIREACGDPEILSLVTAACQDG
                     IPNYFGIQRFGAIRPISHTTGAYILRGDYEAAVCHYIGAAFPGEPEGVREARSSFLEH
                     RDPLRSIREFPLHLNYERTMLHHIASHPWDYRGALRILPPKILSMLVSAYQSLLFNKA
                     LSLRMAEAGSFSEPLPGDRLLFLNGREDRVSAATAGNARIQVARGRCRIAIRMPGCSD
                     KELPCADTTAMEQFLSEDGIQKSDFCTASDLVHARFDGAMRPAALSTKITATLEGDRV
                     TLAFSLQPGQYATTVCREYMKTDPLAMI"
     gene            complement(6033..6749)
                     /locus_tag="PK154_05025"
     CDS             complement(6033..6749)
                     /locus_tag="PK154_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPW10457.1"
                     /translation="MPESPDRPHVLMMSEITADGKLTLKKGSSSKILMKYMDPATEIL
                     LHRTRASLDAIMVGANTIRIDNSYLTVRLVEGKSPIRVIPSSMADIPLDANVLSPDAP
                     TVIAVSEQAPADRVDAIRKRGAEVVCAGQGHVHLPTLMRILRTRYGVRKLMIEGGPTL
                     NWHMLHHRLVDEIRLIHLPFIVGGADTPSLVGGMHIETEEEMIRLVLKDHYLCGTNLV
                     TEYTVLYPPGQLKDEPGRIS"
     gene            6877..7509
                     /locus_tag="PK154_05030"
     CDS             6877..7509
                     /locus_tag="PK154_05030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPW10458.1"
                     /translation="MNITALRPRLVRHVEPIATVFCRAGITPNQISLLSLFGGMLCAV
                     CYFYQMFILGSLFLFISAVLDLVDGSVARRTDRETRFGAVFDWIADKYVDALVLLGVG
                     LSGIAILSQFADFPPVSDFAIVAVAIIGSLMNTFIKPVVYAETGYKDRIGGKIEDPLE
                     GIGFFGRPETILVLIIGGVTGFIWVSVILIALCANLSALQRIIYLYGRLS"
     gene            complement(7481..8287)
                     /gene="artA"
                     /locus_tag="PK154_05035"
     CDS             complement(7481..8287)
                     /gene="artA"
                     /locus_tag="PK154_05035"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HPW10459.1"
                     /translation="MKELFVLASCIFFLAFLIPTMARRYFAILGWTAIVLFLFAEVPY
                     YLSINNFLYPTLAVLAVPFLYITAQYLLAGDPRVEHLSRAAAVAFLIYAPFAFTPLGD
                     WLISVVVGQTLWLVRSLGFGVDLLAWNMVIRNGFRVEIILACTGIQAIAIMLGVAFAI
                     PTTLRQKILSFLLVVPTIYILNLIRNTAVIIAYTEQWFPFLADIAGNGEFGYESFFWA
                     HNVIAELLALVLLVAIAYGLFRIIPGLADLAAGLYDLYSGEVRRVFHRDR"
     gene            8406..8717
                     /locus_tag="PK154_05040"
     CDS             8406..8717
                     /locus_tag="PK154_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HPW10460.1"
                     /translation="MFCPECKSLMISSAGQLKCRKCGYIRKITEKDQLQKTRKRSEKE
                     IVIVEEEGSIPTLPTIAIKCPKCENNLAFWWLRQLRAADESEVRFFRCTVCGHTWRQY
                     D"
BASE COUNT         1924 a         2576 c         2671 g         1804 t
ORIGIN      
        1 ccctgctcgt aggaacggat gaacagggtc cctacggcgt acgccacctt ctccagccgg
       61 taccggtagg ggagggttcg gtcgaaggga ttgaaacagc gggtttcgag cgactgggtc
      121 atcttccgta tcatgtaccc gaagacgtag aggtagcgga gcatcatccc gagcgtgagg
      181 gtgaactcgg cagggaggcc gagccgcccg gctccctcga ggagatcctg ggtccgcgtg
      241 gtggaggaga ggaggatgat gaacgagaca gagacgagga acttaacgag gaggatggag
      301 gcaaactgga ccgattcggc atagatctcg atcccgaacg gcagggtggc gattgtcgta
      361 aaactctcgt agtacgggtt cttgaaaaag atctggaata cgatcaggaa gatgccaaac
      421 ggcaggatga ccgcaagccg tttgaggtag accgcgggag agagccggga gacagtccac
      481 agcacgacaa agaatgcaaa gaagatcgca ccgaccgtgt agatcatggt agagtacggc
      541 acggcaacga tcgcgatgat ggccgcgaaa gcgatgatga tcttgacccg tgcatcgagg
      601 gcatggaccg ggctcttttt atacgcctgc ttctcgagga agaagaggtc ttcgatcatg
      661 cccgttccct gtaggtcttc aggaaggcgg cctttgcttc gtcgagggta tacgccatcg
      721 cgatttcgat cccctcctcg cggagctgcc ggatcagctt ggggatgacc gggacatcca
      781 gccggaccga gctcaggagt tccaggtcgg cgaagacatc cgagacgcga ccctcggcaa
      841 cgatgctccc cctgtgcatc acgtagaggt gatcggcaac ctcggggacc agggagacat
      901 cgtgggtgga gaagatgacg gtgatcccgt actcgcgggc cagggcattg atcaccccga
      961 ccaggtcctt cacccccttt ggatcaagcc cggcggtcgg ttcatcgagg acgatcacct
     1021 gtggctccat tgcgatcacc ccggcgatcg ccacccgctt cttctctccc ccggagagat
     1081 ggtggggtac ccgctctctc aggtgctcga tcccgaccat tgccagggct tcatcgacgc
     1141 gatgattgac ggtctcggca tccagcccga ggttcatcgg cccaaaggcc acgtcctgct
     1201 cgaccgtggg ggagaagatc tggtcatcag cgttctggaa gacgagcccg acaaacttcc
     1261 ggacttcccg gatgttcttc tgggtgatcg gctcgccatg gaccagtacc gtcccgctcg
     1321 ttggccggag gatcccgttg aagtgcttaa aaagggtgct ctttcctgcc ccgttggcac
     1381 ctatgaccgc gatccgtgca tttctcccgg cgataaagtt cacgtgctca agcccggtca
     1441 cattaccggg gtagacgtag gtcagatcgc gggtttcaag gaggtgcatt acaggaataa
     1501 tgtgcgaaaa aaatagataa atggttgtct ttcatccctt gcgggcgacg acccgggata
     1561 aaccgaaggc gatgccgact acgacaaggg tgccgatgat tacggcagca gcctcaccaa
     1621 acttcgcgag tccttcgata ctgtaatcgg ggaatggtga ctcgaaggag tactcgttgc
     1681 cggtgatcgc ggtcacctcc tcctcggctg tgccggccat ctcctcatcg agggtcgaac
     1741 ctctcagatc ctttgctccg aaaattccga agaaggcact ctcgaggccg tcaggattgc
     1801 tggaggcgat aaagggtgcc atagcagcga tgatcagggc aatggcaatc cctgcaagga
     1861 tgaagatctt cttgtccatc atgccggcac caccgttgtg tccgccatga tatcgggccg
     1921 ggccgtggct atcaggtaga tggcaaatgc cgtgataccg ccctcgatga tgccgatgac
     1981 ggcatggtag agtcccatgg agaagagtcc gagttcgagc gggaaggtcc ccgccacggc
     2041 catctccacc gcacaggcca gggctgcaac aaagagcgag agccagccgg caatgaaggc
     2101 gggggcatag ggggacttga agaccggtaa gagcccgcgg tagctgtaat acccgacaaa
     2161 tcctccgatc actcccatgt tgatgatatt ggcccccatc acggtaaggc ccccgtctcc
     2221 gaagagcacc ccctgcacga tcaggacgag cgtgaggatg aagacggctg cgaagggtga
     2281 gccgaggacg atagcggcga gcgcggcacc catcatgtgc ccgctcgtgc cccaggggat
     2341 cgggatattg accgcctgga tggcaaagat cccggcggca aggatggcca cgagcgggat
     2401 cttctcttcc gagagttccc tccttgccca tctgagggcg agggcgatga agatcagggc
     2461 gatgatccag tagactgctg cctggggcag cggaataaac gcatcgggga tgtgcatgac
     2521 gtcacctttt cataataatt taaacattcg tatgaattca tacataaaaa agattattat
     2581 ttgataaatt ggcgtactcc ggagcggaac agtattccca aaacagggta tatcttcgga
     2641 ttttttaaaa tgcgccgcag caggacgccg ggctgatcca tgtccccgta catgaggata
     2701 tcggccttga gagagggatc gttcagcacc ctgatcagct ggtcgacctc ttctcccgtc
     2761 agcttcccgc gggcagagaa gaggcggaag ccgagatcca gttccctgcc cagttctgct
     2821 ctccagcgct gttcgtaccg gcagagcaag ttgtctgcgg cagacccctc gttgcaggct
     2881 tcgcaggtgg tctctgctgc atggtacgcc gaccgtacgc cggtatagac cccgccaccc
     2941 gaggtgggct tcgggaaccc ggcagcatca ccgataaaga gggtcccgtg gccatatgtc
     3001 cggggcatca caccgatcgg aattgtcccg gtcacgaggt ggcagcagct tcgcacgccc
     3061 tgttgcgaga tgaaccgtga gaaccgtgag gcgacctctc tccttccggc aaggccgatc
     3121 cgggccctcc ctttcccgga cgggatgatc caaccgaaga agtcgggcga tgcgtcaggg
     3181 tagatctcga caagggttcc ccccatctcc cgggggacct cggcctggat cccggcaagg
     3241 aagacaggcg gtcgctccat cccgaggtcc cgtgcaaccg ggctccgggg tccgtctgca
     3301 gcgatcagca tacggcacgc gatctcgccc tttccatcca cccctctcgt gataatcgac
     3361 gatcccgatc gcttcaggta tgccgttttg acccggacct cggcaccggc atctgcagcc
     3421 gctgttgcca tctccctgtc gagaagtccc cggtcgacca cgaatgcctt ggtcgtacct
     3481 gcatcaaacg tgagccggga gccccgtgaa gtcacgatac tcgcgccgga gacctcgtgg
     3541 agcaccggtt tttctgagac ctggcacgcg gcaagtgccc tggccgagag gagccccgca
     3601 cactggacgg gatacccgat cgcggcatgc tcctcgatca ggcacgtgga gaggccgagc
     3661 tcggcacacc ggcgtgcgca ggtgctcccc gcagggccgg cacccaccac cacgacatca
     3721 tactggtccc gttctgtcac gatcgtgttc taggttctcc tttcagggca tcaatctgac
     3781 agatccagcc ccgaaaaaag tcctcacccc ctccccatgc agtcctgccg gggataccca
     3841 ctccccgttt ttgtgtgatg ttcgggtgaa cctgcaaacg tactgatcct cgagcaggta
     3901 cccgggcatt ccatcacgaa cgattcagat ggtattgcgg cctcttctgt accctgttcc
     3961 atggccatct atttgatacc ccacgggaac ctagtgtgag agcaatggag aaaggtccgg
     4021 tatttgagtg gaagcagtgc ctggtggtgc gctccgacct caggatgagc tgcgggaaga
     4081 tgtgtgccca ggctgcacat gcatcgatcc ttgcctgcgg ccggtcgaag gctgcctcac
     4141 gcaggcgctg gtgtgccgag gggcagaaga aggtcgtcct gaaggtcgcc ggcgaaaaga
     4201 tcctctttga gctgaaaacc ctggcagagg tggcagggat agcggcagcc ctggtccagg
     4261 atgccgggat gaccgaggtg ccgccgggaa ccatcacggc gctcgggctc gggcctgcaa
     4321 agtcggaaga tctcgacaag atcaccgggg acctcgcgct cctatgagag agagcccata
     4381 tcccctcgag cagctgcttg gtatgcggta ctatgggacc gacacccccg ggacaggggg
     4441 gcggctccgt gctcttcctg aagacttcat tgttgaagag attcccggcg atatcggcac
     4501 ggatgggcgg ttcctgatct gcaggctgac gaaaaaggac tgggaccagc agcgggcgat
     4561 aaaagagatc gcaaaacgcc tcggcatctc gtataaacgg atcgggtttg cggggaccaa
     4621 ggacaagcat gccgtcacct cccagctgat ctcaatcgag gggatcactg cagaggagat
     4681 cgagcaggtc cggatccggg atatcaccct cgaaccggtc gggcgatcct cctcccccct
     4741 tgcactcggc tcccatgcag caaatcgctt caccattacc attcgggaag cctgcggcga
     4801 tccggagatc ctctctctgg ttaccgccgc atgccaggac ggtatcccga actacttcgg
     4861 aatccagcga ttcggggcga tccggccgat cagccatacc accggggcgt atattctgcg
     4921 gggggactat gaagcggcag tctgccacta tatcggggcc gcattcccgg gcgagcctga
     4981 gggagtgagg gaggcacgca gctcgttttt ggagcaccgg gacccgctcc ggtcgatcag
     5041 ggagtttccg ctgcacctga actacgagcg gacgatgctc caccacatcg cgtcccaccc
     5101 ctgggattac cgtggagcac tccggatcct ccccccaaag atcctctcga tgctggtcag
     5161 cgcataccag tccctcctgt tcaataaggc cctgtcatta cggatggctg aagcgggcag
     5221 cttctccgaa ccactccctg gtgaccggct cctctttctg aacgggaggg aagatcgggt
     5281 ctctgccgcg actgccggga acgcacggat ccaggtcgcg agaggccggt gccggatcgc
     5341 catacggatg ccggggtgca gcgacaagga actcccttgt gcagacacaa cggccatgga
     5401 acagttcctc tctgaggacg gcatacagaa gagcgatttc tgtaccgcct ctgacctcgt
     5461 gcatgcccgg tttgacgggg ctatgcgccc tgccgcgctt tcaaccaaga tcaccgccac
     5521 cctggagggg gaccgggtaa cacttgcctt ctcccttcag cccgggcagt acgccaccac
     5581 cgtctgccgg gagtacatga aaactgatcc ccttgcaatg atctgattgc agggggaatg
     5641 atccagggat ctttcccaaa ctcatgctcc gggaaggcac tcgtgattaa aattcccggg
     5701 atcctcaggg tccggcactg acattcagga aactctcccc ggagttgtta ctttggggaa
     5761 tccttcctgg catagtatag ggatatcctt cccgaaagtg atctttaggg gatgatcccc
     5821 tgaagggata cccgggagaa ttcttcaggg gaaattaccc cgggtgtgtt ctccttcagg
     5881 aaaatactcg tgatgtactc tttcagaaca cactccacga gatgtgctga agatacctct
     5941 ctctgagatc tatccttctg agatttgtgg aagatacctc tctctatgat ctgtggaaaa
     6001 aaggaacgag cgtgaacctt ttgagcggtc gttcacgaga tccggccggg ttcatctttc
     6061 agttgcccgg gagggtagag cacagtatac tcggtcacga ggttggtacc gcagagatag
     6121 tgatctttca ggaccagccg gatcatctcc tcctccgtct cgatatgcat acccccgaca
     6181 agtgacgggg tatcagcacc accgacgatg aaggggaggt ggatgaggcg gatctcatcg
     6241 acgaggcggt ggtggagcat atgccagttc agcgtgggtc ctccctcgat catcagtttc
     6301 ctcaccccgt accgggtccg gagaatcctc atgagggtgg ggaggtggac atggccctgc
     6361 ccggcacaca ccacctctgc accccgcttc cggatggcat ccaccctgtc ggccggtgcc
     6421 tgctctgaca cggcaatgac cgtaggagca tcaggggaga ggacattggc gtcaagggga
     6481 atatcggcca tgcttgaggg gatgacccgg atcgggctct tcccttccac cagacggacg
     6541 gtcaggtaag agttgtcaat ccggatcgta tttgccccga ccatgatggc atcaagggat
     6601 gccctggtcc gatggagcag gatctcggtg gcaggatcca tgtatttcat caggatcttg
     6661 ctcgagctcc ctttcttgag ggtcagcttg ccatccgctg tgatctccga catcatcagc
     6721 acgtgggggc gatcaggtga ttccggcacg actacgacct ttgcaggaga agcttcgccc
     6781 tgatcggtta aagaggttgg cccctctgct gctccctgcc gcattcatga cggacgatgg
     6841 caagtattat ccattgagct gctgcttaga taatccatga acataaccgc actccggccc
     6901 cggctcgtcc gtcatgttga gccgatcgcc accgtgttct gcagggccgg catcaccccg
     6961 aaccagatct cccttctctc tctcttcggc gggatgctgt gtgcggtatg ctatttttac
     7021 cagatgttca ttctcgggag tctctttctt ttcatctctg ctgtcctgga tctggtcgat
     7081 ggcagcgttg cccggcggac cgacagggaa acccgttttg gggcagtctt tgactggatc
     7141 gctgacaagt atgtggatgc cctggtcctt ctgggagtcg gcctctcggg catcgcgatc
     7201 ctgtcccagt tcgcggactt tccccccgtc agtgattttg ccatcgttgc cgttgccatc
     7261 atcggctccc tgatgaacac gttcatcaag ccggtggtct atgcagagac gggatacaag
     7321 gaccgtatcg gcggaaagat cgaggacccg cttgagggga tcggcttttt cgggcgcccg
     7381 gagacgatcc tcgtcctgat catcgggggg gtcaccgggt ttatctgggt ctcggttatc
     7441 ctgatcgccc tctgcgccaa cctctcggcg ctgcagcgga tcatctatct ctatggaaga
     7501 ctctcctgac ctcccctgaa tacagatcgt agaggcctgc cgcaaggtcg gcgagacccg
     7561 ggatgatccg gaagaggcca taggcaatag cgacgagaag aaccagggcg agcagttccg
     7621 ctatcacgtt atgagcccag aagaagctct cgtacccaaa ctccccgttc ccggcaatat
     7681 ccgcgaggaa ggggaaccat tgttcggtat aggcgatgat cacggcagtg ttcctgatca
     7741 ggttcaggat ataaatggtg gggacgacca ggagaaacga caggatcttc tgccgcagcg
     7801 tggtcgggat ggcgaacgca accccgagca tgatggcgat ggcctggatc ccggtgcagg
     7861 caaggatgat ctccacccgg aatccgttcc tgatcaccat gttccaggca aggagatcca
     7921 ccccgaatcc aagcgaccgg acgagccaga gcgtctgccc gaccaccacc gagatgagcc
     7981 agtcaccaag cggggtaaag gcaaacggtg catagatgag gaaagcaacg gcagccgccc
     8041 tcgagaggtg ctcaaccctc gggtccccgg caaggaggta ttgtgccgtg atgtacagga
     8101 acggaactgc caggaccgcg agggtgggat acaggaaatt gttgatggag aggtaatagg
     8161 ggacctcggc aaacaggaag aggacgatcg ccgtccaccc aaggatagcg aaatatctcc
     8221 gtgccatggt ggggatcagg aatgcaagaa aaaagatgca tgatgcaagc acgaagagct
     8281 ctttcatccc tgtatggttc gtcgcacaat gaaaaaagga tttccgatag ggcatagtgc
     8341 cgattggaga gcgttccgac gttctccgga gctttaatca tgaatgtgca caaattaatg
     8401 aaccaatgtt ctgtcctgag tgcaagagcc tgatgatatc ctcggcgggc cagctcaaat
     8461 gccggaagtg cgggtacatc aggaagatca ccgaaaaaga ccagctccag aagacgagga
     8521 agcggagtga aaaggagatc gttatcgtcg aggaggaggg ttcgatcccg accctcccga
     8581 cgatcgcgat caagtgcccc aagtgcgaga acaaccttgc attctggtgg ctccgccagc
     8641 tccgggctgc cgatgagagc gaggtcaggt tcttccggtg caccgtctgc gggcatacct
     8701 ggcggcagta cgattgaagt atctccgtct ccgatggaat gaaaagaacg ctgttccgat
     8761 cactttacgc cggctccttt gagcgaaaat ggtcggagcc tggtgaatag tcacgttctc
     8821 ttctttggct ttcgatcttt caggggggca taggatcccc gaagatgctg atcccgtccc
     8881 gccgtaagac acatcagcgg gatggttttt ttagtatgaa tccctggagg gccaccggca
     8941 tacacgatga gaatgccgga ggcagtttta ttcga
//
LOCUS       DAOOCO010000065         8947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7368_length_8947_cov_4.914305, whole genome shotgun sequence.
ACCESSION   DAOOCO010000065 DAOOCO010000000
VERSION     DAOOCO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_8947_cov_4.914305"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..1443
                     /gene="argC"
                     /locus_tag="PK154_05045"
     CDS             457..1443
                     /gene="argC"
                     /locus_tag="PK154_05045"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPW10461.1"
                     /translation="MKIAIIGASGYAGGDIIRLLLSHPRAEVTCATSRKLAGKPLDSV
                     HPHLKGYTNLNFENPSLDSIDADVAFLSVPHTAAMTYARPLLDRGIKVVDLSADYRLP
                     KETFERVYGVAHTDYFEAPYGIPELHRDEYRSAAFVSNPGCFPTGATLAAAPLAPYAH
                     TVIYDSKTGVSGAGDNPSATTHYPNVADNINPYKWTAHRHLAEMRNEISRINKKARCY
                     FTPHLVPVNRGILTTAHILLNEPMDQETVEDLYRKFYAREYFVRYQNPVLAAVRGSNF
                     CDIRAESEGDRVVVVSAIDNLGKGAAGQAIQNMNIICGFDERDGLRAPGLLP"
     gene            1459..1932
                     /locus_tag="PK154_05050"
     CDS             1459..1932
                     /locus_tag="PK154_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW10462.1"
                     /translation="MKVREIMTRNPVTVSADEPVRTAAGLLRKNRIGGLPVMEQDTVV
                     GVVTESDIISLLETGKLSDDLWLPSPLEVIEVPLREIVNWEKTKTALTNIGDTPVKKI
                     MSHPPITIDEEADIEDAAALMLSHKIARLPVVQQGRLTGIVAKADIIRGVGGAID"
     gene            1951..3102
                     /gene="argJ"
                     /locus_tag="PK154_05055"
     CDS             1951..3102
                     /gene="argJ"
                     /locus_tag="PK154_05055"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HPW10463.1"
                     /translation="MKSICGVEGVRAYGIKEGKFGLALIEANGAAAGVFTANLVRAPP
                     VTLMEERIRKGHLGAVIVNSGCANAYTGKQGYHDAIRMGEIAGRKLGIDPEHVGVAST
                     GVIGRYLDLPRIEDQCHRIAPLMEHSTLAETLAARAIMTTDLTEKHALIRGEGFSVGG
                     ITKGSGMIAPNMGTMLAFLYTDADISASSLSTALRQAANRTFNRIVVDGDMSTNDIAL
                     CTATGCAGSPDPGEFSRALEECCRSLAIQIAMDGEGATKLLEVTVNGAPSEEEAATVA
                     RTIVSSPLVKTALYGEDPNWGRVIAAAGRAGVRFDPDGVDLVISDGSTSCPLVCDGKI
                     VVDLAAAKKAMQGKKVIFFLDIHNGPGSATAWGCDLTEKYVEINGRYTT"
     gene            3099..3971
                     /gene="argB"
                     /locus_tag="PK154_05060"
     CDS             3099..3971
                     /gene="argB"
                     /locus_tag="PK154_05060"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPW10464.1"
                     /translation="MKREEVLTEALPYIQQFHGKTMVIKLGGHAMVDGIILDKAISDV
                     VLLQLVGIKVVLVHGGGPEITEKMKALGKQPKFVGGLRITDEETLEIAQMVLVGKINS
                     NIVSLIAKCGGKAVGISGNDGNLIIARKMTPQKVRVDGREQEVDLGHVGEIEEINPAV
                     LNTLLDNRYIPVISPIAIDRNGQNLNINADTAAGEIAVALNAYKLINMTDVEGIMDAS
                     RTKVYRRLKASEAGELISLGVVAEGMIPKVSSIVNAVTGGVQFGHVINGNVAHNLLLE
                     MFTDEGVGTMIYRD"
     gene            3998..5149
                     /locus_tag="PK154_05065"
     CDS             3998..5149
                     /locus_tag="PK154_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPW10465.1"
                     /translation="MLQRILKYGILLIILGLVSLPCVAAETPGGESSTGHLAVNNGTF
                     SPVGFEEGKPVIYFFFNQNCGECVKTHPFIDEFGRNRPDVVIHPIDIMESDENLALFH
                     SFRDYYGTGFIPVPAVFVRNISLTGYEEITMGLADAVNGTWTNGSGEEVIPDGESPRS
                     TLTVPLIILAGLADGINPCAFAVLIFLILSLISLESRSKMLVVGSVFIFAVFCFYFMS
                     GLGIFAVVQSAGISQIISIVAAVIAIAAGLYSILSVAGGEKGPALLSIPERGKSIIEP
                     YIRKASIPAAFIVGILVGMFELPCTGGIYLAILSLLSNRMSIMEGIPYLLLYNLFFIL
                     PLVIILGVFAFGLPVERLEQLQTGSRRGVRVLMGFVMILLGIILIFEIL"
     gene            complement(5365..5649)
                     /locus_tag="PK154_05070"
     CDS             complement(5365..5649)
                     /locus_tag="PK154_05070"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPW10466.1"
                     /translation="MPIDALRAEINRIDLQLIDLIAERQKIAGKIAQIKTNEGLPIHD
                     RKRTHEVLEGAFNAAVEKNINPVFVQKIFELLIEMSEERQRECSGEGNLP"
     gene            5745..6392
                     /locus_tag="PK154_05075"
     CDS             5745..6392
                     /locus_tag="PK154_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HPW10467.1"
                     /translation="MLERISRRERYDLLIAWFAISIAFSLAFTGGVGTWMGNPLQETT
                     LQTYLVFLVLSMVTVGIGFVLHELAHKFTAMHFGFWAEFRKDNLMLLVAVVLAALVGL
                     VFAAPGATMIYGSGVTREQNGKISAAGPLTNLVLCIPFAVLLFASTVLGPAASSGGLL
                     TMVGVVGLQVNAMIAAFNMLPVSVLDGKKVLAWNPALFAILIVAAFGILIGTFYL"
     gene            6496..7188
                     /locus_tag="PK154_05080"
     CDS             6496..7188
                     /locus_tag="PK154_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPW10468.1"
                     /translation="MHIMEGYLPWQWCVVWWIIALPCLVFGIYRLKKVLEMDREAFPL
                     LGVTGGFIFILSSLKLPSITGSCSHPTGTGLSAVCFGPWITSVVCAIVLLFQSLFLAH
                     GGLSVLGANVVSMGVIGPIAGYIVYRALRDTRVNIYLTVFLACAIADLFTYITTSLEL
                     ALATPAEVGGITSSFVLFMGIFAFTQVPLAIVEGIVLALVFKYIVQLKPDIILRLKVF
                     PEQKIRAARGEA"
     gene            7188..7490
                     /locus_tag="PK154_05085"
     CDS             7188..7490
                     /locus_tag="PK154_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transport protein CbiN"
                     /protein_id="HPW10469.1"
                     /translation="MGFKYRLEILTILAILGFCALFLYTSSIMTEAEFAGADTQGSAL
                     VAEITGKSDEEFHPLIWQWSPPSGEIEAGIFALQAAIGGIMVGWVFGYWKGQKKTV"
     gene            7512..8312
                     /gene="cbiQ"
                     /locus_tag="PK154_05090"
     CDS             7512..8312
                     /gene="cbiQ"
                     /locus_tag="PK154_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPW10470.1"
                     /translation="MYEEFLEDIAQTNGLREVNTYLKLLAGLGAILLCLLSQSYIGPL
                     FIAIVLSVTILSLAKIDARTYGGLFILPFTFAIMSVAVIILISGGEETFWSWNPLPWL
                     SLSVTEESLNEGFFVFCRVIGGMSALIFIALTTPMTDLFVVMRQCRVPEVVLDLAMMI
                     YRSIFMIMDQLVQTYHAQVMRLGYGSFRESIQSFSTLCGAVFIGSWSAGEDLIRAMDA
                     RCYDGKFAVLGETRPIEVLPFITVALFLGVSSVVVFLARDLPLLGAGS"
     gene            8309..>8947
                     /locus_tag="PK154_05095"
     CDS             8309..>8947
                     /locus_tag="PK154_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10471.1"
                     /translation="MSRIILEARDIRYRYPRGMEAIRGISFHIRRGEKIAVVGPNGAG
                     KSTLLLLFNGMIRPDAGMMLFDNEPFGYDKASLRTLRKRVGFVLQNPDRQIIAPTVFQ
                     DVAFGPVNLGYSERDVKHAVEGALRYVGLEGFERRPPHQLSGGEKKRVAIAGVLAMDP
                     DVLIIDEPTSGLDPSGSEDIMELLDELNTQGKTIIISTHDIELAYPWADRAIL"
BASE COUNT         2059 a         2270 c         2439 g         2179 t
ORIGIN      
        1 aaatgattct ggcatgtaag gttactcctt atccgtgtac gatccttcca tcgtcaatca
       61 attcctgcat ccgagaggat tcatcggagt cccggtcggt ccgggagcca tcatcccctg
      121 atgcattcct ccttatgaag attgtcatgt tgttcgtctt accgtgcagt gaggcagacc
      181 cctgggtcag cctcaggttg gagctgcatc ccctaatttt tctccatccc tccgggtgta
      241 ttgtaatgat taatcattat aataaaaaaa cctgctgaat ttaggtgacg gcgtgagagg
      301 atggaccact cttttacgat ccttctttag gagctgtctg atgatgaagg cgcgcaatcc
      361 tctgtctgca gaaaaaaagg agagcagaat ggattttatg attaattcct ttttccgggg
      421 aaaaagcatt aaaatcctca cgccgaatag gtttttatga agatcgcgat tattggggca
      481 tcgggttatg ccggcggaga tatcatacgg ctgttactct cccatcccag ggcagaagtg
      541 acctgtgcta cctcccggaa gcttgcagga aaaccgctgg attcggtgca cccccacctg
      601 aaggggtata ccaatctcaa ttttgaaaat ccttccctcg attccatcga tgcagatgtc
      661 gccttcctct cggttcccca tactgccgca atgacctatg cacgaccgct cctcgaccgc
      721 ggaatcaaag tcgtcgatct ctctgcggat taccgtctgc cgaaggagac gttcgaaagg
      781 gtgtacgggg tcgcccatac cgattatttc gaagcccctt acgggatacc cgaactgcac
      841 cgcgatgaat accgctcggc agcatttgtc tcaaacccgg ggtgtttccc cacgggagct
      901 acccttgcgg cagcccccct cgccccctat gcccataccg tgatctacga ttcgaaaacc
      961 ggggtgagtg gtgctggaga caatccttcg gcaaccaccc actatccgaa tgtggccgat
     1021 aatatcaatc cgtacaaatg gaccgctcac cggcacctcg cagagatgcg gaatgagatc
     1081 agccgtatca acaaaaaggc ccggtgctat ttcacccctc acctggttcc ggtcaaccgc
     1141 ggaatcctca caacggcgca catcctcctg aacgagccga tggatcagga gacggtggag
     1201 gacctctacc ggaagttcta cgccagggag tatttcgtcc ggtaccagaa cccggtgctt
     1261 gccgcggtcc gggggagcaa tttctgcgat atcagggcag agagcgaggg agaccgggtg
     1321 gtggtggtct ctgccattga caatcttggt aaaggggcag ccggacaggc gatccagaac
     1381 atgaatatca tctgtggttt tgacgagagg gacgggctcc gggctcccgg gttgctcccc
     1441 tgagaggagg aaacagaaat gaaagttcgg gaaatcatga caaggaatcc tgtgacggta
     1501 tcggcagatg agccggtccg cacagcagca gggcttcttc gaaaaaaccg tattggcgga
     1561 ctgccggtca tggaacaaga taccgtggtc ggtgtggtga cggaatccga tatcatctcc
     1621 cttcttgaga ccgggaagct ctccgatgat ctctggctcc cctctcctct cgaggtgatt
     1681 gaggttccgt tgcgtgagat tgtgaactgg gagaagacca agaccgcact caccaatatt
     1741 ggggacacac cagtaaaaaa gatcatgagc caccctccca tcaccatcga tgaggaggca
     1801 gatatcgagg atgcagctgc tctcatgctc tcgcacaaga ttgcccgtct ccctgtggtg
     1861 cagcagggac gcctgacagg tatcgttgca aaggcggata tcatacgcgg cgttggtgga
     1921 gctatcgact gaaataccgg agaaaagagc atgaagagta tctgcggtgt ggaaggagtc
     1981 agggcatatg gcatcaagga aggcaagttc ggccttgccc tgattgaagc caatggtgca
     2041 gcagcgggag tgttcaccgc aaacctggtg agggcacccc ctgtcaccct catggaggag
     2101 aggataagga aggggcatct tggtgccgtg atcgtgaatt ccggttgtgc gaatgcatat
     2161 accgggaagc agggctacca cgatgcgata agaatgggtg agattgccgg ccggaagctt
     2221 ggcatcgacc cggagcatgt cggtgtcgcc agcaccgggg tgatcgggcg atatcttgac
     2281 ctcccccgga ttgaggacca gtgccaccgg attgcccctc tcatggagca cagcactctg
     2341 gcagagaccc ttgcagcacg agctatcatg accaccgatc tgacggagaa gcatgcgctt
     2401 atcagggggg aaggattctc tgtcggagga ataacgaagg gaagcgggat gattgcaccc
     2461 aacatgggga cgatgctggc attcctttat accgatgcag acatctccgc gtcctcgctc
     2521 tcaacggcgc tgaggcaggc cgcaaaccgg acgttcaacc ggattgtcgt ggacggtgac
     2581 atgagtacga atgatatagc gctctgtacc gccaccggct gtgccgggtc ccctgatccg
     2641 ggggaatttt cacgggctct cgaagaatgc tgtcggagtc ttgcgattca aattgcgatg
     2701 gatggggaag gtgcaacgaa gctgctcgag gtaaccgtca atggcgcccc ttcagaagag
     2761 gaagctgcga ccgtggcacg gacgatcgtc tcatccccgc ttgtaaaaac tgcactgtat
     2821 ggggaagatc ccaactgggg aagagttatt gctgctgcag ggagggccgg agtccgtttt
     2881 gatcctgatg gggtggatct cgtgatcagt gacggatcca cttcctgccc ccttgtgtgc
     2941 gacgggaaga ttgtggtcga tcttgcggcc gcgaaaaaag ccatgcaggg gaagaaagtg
     3001 atctttttcc ttgatatcca caacggtccc gggtctgcga cggcatgggg ctgtgatctg
     3061 actgaaaaat atgtggagat caacgggagg tataccacat gaaacgcgaa gaggtcctga
     3121 ccgaagcact cccctatatc cagcagttcc atggaaagac gatggtgatc aagttggggg
     3181 gccatgcgat ggtggatggc atcatcctcg acaaggcgat ctctgatgtc gtcctcctcc
     3241 agctggtggg gatcaaggtg gtgcttgtcc acggcggtgg tcctgagatc acggagaaga
     3301 tgaaagcgct cgggaaacag ccaaaatttg tcgggggtct caggatcacc gatgaagaga
     3361 cgcttgagat tgcacagatg gtccttgtcg gcaagatcaa cagcaatatt gtatccctga
     3421 ttgcaaaatg cggtggaaaa gcggttggta tatctggaaa tgatggtaac ctgatcatag
     3481 ccagaaagat gaccccccag aaggtcaggg ttgacgggag agagcaggag gtcgatctcg
     3541 ggcatgtcgg tgagattgag gagatcaatc ccgcggtgct gaacaccctc ctcgataacc
     3601 ggtatatccc ggtgatctcc cccatcgcca tcgatcgcaa cgggcagaac ctgaacatca
     3661 atgctgacac ggcagcaggc gagatcgcag ttgccctgaa tgcttacaag ttgatcaata
     3721 tgaccgatgt ggaagggatc atggatgcat ccaggactaa ggtataccgg cggttgaagg
     3781 catccgaggc cggggaactg atcagcttgg gagtagttgc tgaggggatg atcccgaagg
     3841 tgagctcgat cgtgaacgcg gtaaccggtg gggttcagtt tggccatgtc attaacggaa
     3901 atgtcgccca taacctcctc cttgagatgt ttactgatga gggtgtgggg accatgattt
     3961 accgggattg atccgagtag aggctgtgag gggaaggatg ctacagagaa ttttgaagta
     4021 tggtattctg ttgattatcc ttggcctggt ctctctccct tgtgtagctg ccgagactcc
     4081 aggtggagaa tcatcaaccg gacatctggc agtaaacaac ggaaccttct ctcctgttgg
     4141 atttgaagaa gggaaaccgg tgatctattt cttcttcaac cagaattgtg gagaatgcgt
     4201 gaaaactcat ccttttatcg atgaatttgg aaggaaccgc cctgatgtgg tgatccaccc
     4261 catcgatatc atggagagcg atgaaaacct tgcactcttt cattccttca gggactatta
     4321 cggtaccgga ttcatccccg ttccggcggt ctttgtcagg aatatctccc tcacaggtta
     4381 cgaggagatc accatggggc ttgccgatgc tgtcaatggc acatggacga atgggagcgg
     4441 ggaggaggta ataccggatg gggagagtcc gcgaagcacg cttactgtcc cgctcatcat
     4501 attggcaggt cttgcagacg ggataaatcc ctgtgctttc gcagtcctca tcttccttat
     4561 cctatctctg atctccttag aatcccgttc aaagatgctc gttgtcggct cggtattcat
     4621 ttttgcagtc ttctgtttct atttcatgtc cggtctcggg atttttgccg ttgtccagtc
     4681 tgcgggaata tcacagataa tctcgatcgt tgccgcagtg attgcaatcg cggcaggcct
     4741 ctatagcatc ctgtcagtag ctggcggtga gaaaggtcct gccctgcttt cgatacccga
     4801 acgcggaaaa agtatcatag aaccgtatat caggaaagca tcaatccctg ctgcgttcat
     4861 tgtcgggata ctggtaggga tgtttgagct gccctgcaca ggtggcatct accttgctat
     4921 cctctcctta ctctcaaacc ggatgagcat catggaaggt atcccttatc ttctcctcta
     4981 caacctcttt ttcattctgc ctctggtgat aatcctggga gtatttgcct tcggccttcc
     5041 tgtcgagcgg cttgaacagc tgcaaaccgg atcacgccgg ggggtcaggg tactaatggg
     5101 atttgtcatg attctgctcg gaatcatcct gatcttcgag atactctgat ggtggggtaa
     5161 caaacacaca cccctcactg gcattttttt aaaaaccagt ccttctacca ggaaacaata
     5221 gaagacacac ggaataaaag aagaacggcc cgaattcctg ataatcccct gaatactgcc
     5281 ggatattcac cgggacagtt ctgttgcagg cgggagcagg aaaacagaaa cgggatgggg
     5341 cggaggagaa aaccgtttcg tttttcaggg gagatttccc tcgcccgagc actcgcgctg
     5401 tcgctcttcg ctcatctcga tcagaagttc aaagatcttc tgcacaaaga cgggattgat
     5461 attcttctcc actgctgcat taaatgcccc ttccaatacc tcatgggttc tcttccgatc
     5521 atggatgggc agtccttcat tggtcttgat ctgggcgatc tttcctgcaa tcttctgtcg
     5581 ctcggcgatg agatcgatca actgcaggtc gatcctgttg atctccgctc tcaaagcatc
     5641 tatcggcata gcatatagta acggcacggt agggaattta aatctggtgc tcttctgtga
     5701 taacagatat cccttatgaa tgccaacaga taagggaagg acccatgctc gaacggatct
     5761 cacgaaggga gcggtacgat ctcctcatcg cctggtttgc catctccatc gcattctcgc
     5821 tcgcttttac cggcggggta gggacatgga tgggaaatcc cctacaggag acgacactgc
     5881 aaacctacct ggtcttcctc gtcctctcga tggttaccgt cgggatcggg tttgtcctcc
     5941 atgaactggc gcacaagttc actgcgatgc atttcggatt ctgggcagag tttcggaaag
     6001 acaacctcat gctcctcgtt gcagtagtgc tcgcagccct cgtgggactc gtctttgccg
     6061 caccaggtgc aactatgatc tatggttcgg gtgtcaccag ggagcagaat gggaagatct
     6121 ctgccgccgg cccgttgacc aacctcgtcc tctgcatccc ttttgccgtg ctgctctttg
     6181 ccagcaccgt cctgggtcct gcagcctctt ctggaggcct gttgaccatg gtcggggttg
     6241 tgggtctgca ggtgaacgca atgattgcag ccttcaacat gctcccggtc agtgtgcttg
     6301 acgggaagaa ggtgctcgca tggaacccgg cactctttgc aatcctcatt gtggcggcat
     6361 tcgggatact gatcgggaca ttctacctct gatcaacacg ttcctgtttg gaatgaaccg
     6421 gaagcaagga ataaatagcc caacaataat acaaataaat ttgttttaac gcaaaaaaaa
     6481 ttgaggcgag ttgaagtgca cattatggaa ggatatctac catggcaatg gtgtgtggtg
     6541 tggtggatta tcgcattgcc ttgcctggta ttcggcattt accggctgaa aaaagtcctt
     6601 gaaatggacc gcgaagcgtt tcctctcctt ggcgtaacag gaggattcat ttttatcctc
     6661 tcttcgctga agctcccatc gataaccggg agttgttctc atccaactgg aacagggcta
     6721 tcagcggttt gctttggacc atggatcacc tctgtcgtct gcgcaatcgt gctccttttc
     6781 cagagtctct tcctcgccca tggcggatta tcagtactcg gagcgaatgt cgtctcgatg
     6841 ggagttatag gacccattgc cgggtacatt gtataccgtg ccctccggga tactcgggta
     6901 aatatatatt tgaccgtttt tcttgcttgt gcaattgcag acctgttcac ctatattacc
     6961 acttcattag aactagcctt ggcaaccccc gctgaagtag gtgggataac aagctcgttt
     7021 gtactattta tgggcatctt tgcctttaca caggttcccc tcgcaattgt tgaaggaatc
     7081 gttctcgcgc tagtgttcaa gtatatcgtc cagctcaaac ctgatataat tttacggctg
     7141 aaggtatttc ctgaacagaa aatcagggca gcccggggtg aggcctgatg gggttcaaat
     7201 acaggctgga gattctcaca attcttgcga tactgggatt ctgtgccttg ttcctttaca
     7261 cgagctcaat catgactgaa gcagaatttg ccggagctga tacccagggg tccgcgctgg
     7321 ttgcagagat aaccgggaaa tcggatgagg aatttcaccc cttaatctgg cagtggtctc
     7381 ctccgagtgg tgaaattgaa gcaggaatct ttgcacttca ggctgccatc ggagggataa
     7441 tggtcggctg ggttttcggc tactggaagg gtcagaagaa gactgtataa atcgacaata
     7501 gtgtttcctg catgtacgaa gaattcttgg aagatattgc ccagaccaac ggtctcaggg
     7561 aagtgaatac ctatctcaaa cttttagccg gactgggggc tatccttctt tgtcttctct
     7621 cccagagcta tatagggccc cttttcatcg caatcgtcct ttccgttaca attctctcac
     7681 tagcaaaaat tgatgcaagg acatacggcg gactctttat attacctttc acatttgcga
     7741 tcatgagcgt cgcagtgatc atcctaatct caggagggga agaaacattc tggagctgga
     7801 atcccctccc ctggctctcc ctgtctgtca cagaggagag tctcaatgaa ggattctttg
     7861 tattctgccg ggtaattggg gggatgtcag ccctcatctt catcgctctc actactccaa
     7921 tgaccgatct ttttgttgta atgaggcaat gccgagttcc tgaagtggtc ctggatctcg
     7981 ccatgatgat ataccgcagt atcttcatga taatggacca gctagtccag acatatcatg
     8041 cccaggtgat gcgcctgggg tatggatcgt tccgtgaatc gatccaatcc ttctctactc
     8101 tgtgcggagc agtattcatc ggaagctgga gtgccggtga agatcttatc cgtgccatgg
     8161 atgcacgctg ttatgatggg aagtttgcag ttctggggga gacacgcccg attgaggtgc
     8221 tccccttcat cacggtggca cttttccttg gagtctcttc tgtcgtggtc ttccttgcaa
     8281 gggatctccc cctcctgggg gcgggatctt gagccgaatc atcctcgagg cacgggatat
     8341 ccggtaccgg tatccccggg gcatggaagc gatcaggggg atcagtttcc atatcaggag
     8401 gggagagaag atcgccgtcg taggaccaaa cggagcaggg aaatcaaccc ttctgctgct
     8461 gttcaatggc atgatccgcc ccgatgcagg gatgatgctc tttgacaatg agccgttcgg
     8521 gtatgacaag gcctcgttgc gcaccttgcg gaagcgggtg ggttttgtcc tccagaaccc
     8581 ggaccgccag atcatcgccc cgaccgtgtt ccaggatgtc gcattcggcc cggtaaacct
     8641 cggctacagc gagagagatg tgaagcatgc ggtggaggga gcactcaggt atgtcggtct
     8701 cgaaggattc gaacggcgcc cgccccacca gctctccgga ggcgaaaaga aacgggtggc
     8761 gatcgcagga gtcctcgcga tggaccctga tgtcctcatc atcgatgaac cgacaagcgg
     8821 ccttgatccc tcgggatctg aggatatcat ggaactcctg gatgaactca acacccaggg
     8881 aaagaccatc atcatctcca cccatgacat tgaacttgcc tatccctggg ccgaccgggc
     8941 gatactg
//
LOCUS       DAOOCO010000066         8807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7513_length_8807_cov_4.877285, whole genome shotgun sequence.
ACCESSION   DAOOCO010000066 DAOOCO010000000
VERSION     DAOOCO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_8807_cov_4.877285"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK154_05100"
     CDS             <1..563
                     /locus_tag="PK154_05100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW10472.1"
                     /translation="ICAVGEGVRRATREIPLRPNVVSPLKRENSGDNTGPGMPIVHVR
                     PGSCLSVTVLPKGAGAENMSRLAMLLPTEREKIPRFVAETMLIAGGRPCPPVILGVGI
                     GGTFDGCAALAKEALLLPIDTMDPFEQELCDAVNALGIGPMGLGGKTTALAVKVRMAA
                     CHTASLPVAVNVQCWAARHCTVEVTW"
     gene            557..1105
                     /locus_tag="PK154_05105"
     CDS             557..1105
                     /locus_tag="PK154_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HPW10473.1"
                     /translation="MVTRTSLTTPLGDEVLSLHAGDQVLLSGIVYTARDEAHLMMKES
                     GIPFDPVGAAIYHCGPVIKDARILAAGPTTSARLNALTGFLLDRGVRALIGKGGMGAE
                     VARALIGRGVYLSCTGGCAVLAASKMSLKGVYFEELGMAEAIWIIELDSLPLVVGIDS
                     HGNDLFADIALGANKQFHRTFT"
     gene            1126..1398
                     /locus_tag="PK154_05110"
     CDS             1126..1398
                     /locus_tag="PK154_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW10474.1"
                     /translation="MRLQIDERRCKGCNLCTLVCPYRIFKPGKRANRRGIVVPELDRP
                     ERCANCRLRHLYGRTLCGNCQMICPDQAISWKEDEEPVPKKVAIEY"
     gene            1395..2495
                     /locus_tag="PK154_05115"
     CDS             1395..2495
                     /locus_tag="PK154_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPW10475.1"
                     /translation="MTRTEFMQGNTACAEGALAAGCRFFGGYPITPSTEIAEHMALRL
                     PKMGGVFIQMEDEIASIAAVIGASWTGAMAMTATSGPGFSLMMENIGYAVMTETPCVI
                     VNVQRGGPSTGQPTLSAQGDMLQCRFGSHGDYSVIALSPASVQEMFDLTVEAFNLAER
                     FRSPVFLMADEIIGHMRERVIIPDHVPSNQRKPYEPGSLPFRAGEDLVPGFPAFGKGY
                     GVHVTGLTHDERGYPCATSPKLHEDLVKRLVGKVEGARGSITSCDILNPDAEVVFVSY
                     GAPSRTVQQVLHDHPDDNIGHLRLKMVWPFAEMVLARFTNAEAFIVPEQNLGQIAREI
                     ERHTDKDVVTVPKLGGDLHTPGELYAIVENLK"
     gene            2492..3280
                     /locus_tag="PK154_05120"
     CDS             2492..3280
                     /locus_tag="PK154_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW10476.1"
                     /translation="MSEEDWLRQDRLPHIFCAGCGNGTVINCTLSAVEQIGWKRQETV
                     FVSGIGCSSRAPGYISTDSLHTTHGRALAFATGIKLANPQLNIVVFTGDGDLAAIGGN
                     HFIHACRRNIDLTVVCLNNMIYGMTGGQGSPTTPMGGLSTTTPYGSGEPAFDLCGLAT
                     AAGANYVARWTSYHVRELTRAILTGLENPGFSFIESLVQCPTNYGRRNRFKQVADQIE
                     YFKSHTILVEKAARMREKGEMIPPEMILAGEFVRRNRPALGVRP"
     gene            3277..3816
                     /locus_tag="PK154_05125"
     CDS             3277..3816
                     /locus_tag="PK154_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HPW10477.1"
                     /translation="MRHEIRFSGFGGQGIILAAVILGRAAVMYDNKYAVQTQVYGPEA
                     RGGASMSAVIIDDEPILYPKVATPDIYVIMSQEGFEKYGIAATGEAQMLVDSTLVHSR
                     PSCHYIEIPATREARESLGRDIVANIIMLGALVAATGIVSRSAIEKAILDSVPKGTEG
                     LNVKAMTRGFELAEEAISR"
     gene            3813..4898
                     /locus_tag="PK154_05130"
     CDS             3813..4898
                     /locus_tag="PK154_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HPW10478.1"
                     /translation="MKFLEHEAKSVFSETGIQVPRGFLIHSADELAGYLEQLPPEFVL
                     KAQVDVGGRGKAGGILMADRKNGIENAKALFGKSIKGVAVKEILAEERLEIQHEYYAS
                     IAVDRSTKEALLLFTEEGGVDIEILARENPSAIRRASLPLLMTEIPPFMLRDLLGGAP
                     REVGKVLQSLYTAFCRKDAILAEINPLVATPAGLFAADAKLIVDDNALRRQGISCNRD
                     MTDREREAERHGFSYVELEGNIGVIGNGAGLTMATLDLISFFKGKAANFLDVGGGADQ
                     ERVMHAVRLVAGVPSVKVIIVNLLGGITRCDEVARGIIAAGISQEVFVRLAGTNEEEG
                     RSLLSERGYRMVGSMEEAVRDAVEAVR"
     gene            4895..5761
                     /gene="sucD"
                     /locus_tag="PK154_05135"
     CDS             4895..5761
                     /gene="sucD"
                     /locus_tag="PK154_05135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPW10479.1"
                     /translation="MIYADKSAGIIVAGATGRQGGFHIALMNEYAKTVGGAGVVAGVT
                     PGKGGQVASGVPVYNSIREAMADIDAEVSVIFVPAAAAGDSIMESANAGLSLAVTITE
                     HIPVHDTMNAIAFAKLHDCAVIGPNGPGLLSPGEIKLGIMPSHLFSRGNIGVISRSGT
                     LTYEIVDELTKAGLGQSTVVGIGGDPVVGQTFSDVLRRFEEDPQTKAVVLIGEVGGAL
                     EEEGARSTDLPIAAYIAGISAPPEKRMGHAGAIIEGGEGDARSKIERLRKMEVPVASR
                     PSEIPGIIKKIL"
     gene            7283..8485
                     /locus_tag="PK154_05140"
     CDS             7283..8485
                     /locus_tag="PK154_05140"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPW10480.1"
                     /translation="MKRNIRVEALHQIPLERQGIELVERKCLGHPDSLADGVAEAISR
                     ALSLAYLEECGAVLHHNTDQGEIVAGESIPKYGGGKVIRPIYMLIDGRATRQFNGINI
                     PTDAIALETARKYFRTTLTNLNLERDITIDCRLGTGSTDLRDVFKPCQGTAPRANDTS
                     FGVGHAPFSETETIVLGLSDYIDTTLRARYPAIGQDIKIMGLRAGDTITLTIASAMVD
                     RYCSGLSQYIEYKDILKEHAEKVARKITSRKVSVQVNTADDIDTGSVFLTVTGTSAEM
                     GDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHIGKIYNILATMLAKECVKEVDGI
                     EEVYIRLLSQIGHPIDQPLVASIQVLPREEVHVSEISRDIEGVIDSGLANITCITEKI
                     IRGEVRTF"
     gene            8532..>8807
                     /gene="hisG"
                     /locus_tag="PK154_05145"
     CDS             8532..>8807
                     /gene="hisG"
                     /locus_tag="PK154_05145"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW10481.1"
                     /translation="MVRLAIPNKGRIAAPVMDLMEKSGLHLLDREERKLISRTPDPHI
                     EVLFARPIDIPEYVANGAADLGITGRDMVCERQAEVDELLDLRFGNAT"
BASE COUNT         2163 a         2243 c         2450 g         1951 t
ORIGIN      
        1 tcatttgtgc tgtaggagag ggagtacgaa gggcaacaag ggagataccc ctccgaccta
       61 atgtggtatc cccccttaag agagagaatt ccggagataa caccggtccc gggatgccaa
      121 ttgttcacgt ccgaccgggc agttgtctct cggtaactgt gcttcctaag ggagccgggg
      181 cagagaatat gtcacggctt gcaatgctcc tccccacaga gagagagaaa attccccggt
      241 ttgtcgctga gacgatgctg attgccggcg gaaggccatg ccctcctgtt atcctgggag
      301 tggggatcgg cgggactttt gatggctgtg ctgcacttgc caaggaggct ctgctccttc
      361 ccatcgatac catggacccg tttgaacagg agttgtgcga tgccgtcaat gccctcggga
      421 tcggccctat ggggcttggt ggtaagacga cggcacttgc agtaaaagtc aggatggccg
      481 cgtgccacac ggcatcgtta cctgttgcgg tgaatgtcca gtgctgggct gcccgccatt
      541 gcacggtaga ggtgacatgg tgaccagaac ctccctcacc actcctcttg gagacgaagt
      601 cctctccctc catgccgggg accaggtatt gctctcgggc atagtgtata cggcgcggga
      661 tgaggcacac ctcatgatga aagagagcgg catccccttt gacccggtcg gagcagccat
      721 ctatcactgc gggccagtca tcaaggatgc acgaattctt gcagcagggc cgaccacttc
      781 tgcacgattg aatgccctta ccggattcct tttagacaga ggagtgcgtg cgctcattgg
      841 taaagggggt atgggggcag aggttgcacg agcactcata ggcagaggag tatatctctc
      901 ctgtaccggg ggatgtgcag tgcttgctgc ctcgaaaatg tcattaaaag gcgtctattt
      961 tgaagaactt ggaatggcgg aggccatctg gatcatcgaa cttgattcac ttcccctcgt
     1021 tgttggtatt gactcccatg gcaatgacct ctttgctgat attgccctgg gggcaaacaa
     1081 acaattccac agaacgttca cataaacaca acaaaggacc atgccatgag actgcagatt
     1141 gatgaacggc ggtgcaaggg ctgcaacctc tgcaccctgg tctgtcctta ccggatcttc
     1201 aagccaggaa agcgggctaa ccggagaggg atcgtcgtcc ccgaactcga tcgtcctgag
     1261 cggtgtgcca actgccggct gagacacctg tacgggagaa ccctctgcgg gaactgccag
     1321 atgatctgcc ctgaccaggc tatctcgtgg aaggaagatg aggaaccagt accaaaaaag
     1381 gtggcgatag agtattgacc cggactgaat tcatgcaggg aaacacagcc tgcgcggaag
     1441 gggcactagc cgccggttgt cgtttttttg gcggctatcc gatcacccct tcgaccgaga
     1501 tagcagagca tatggcgctt cgcctgccaa aaatgggcgg ggtgttcatc cagatggagg
     1561 acgagattgc gagcatcgct gctgttatcg gggcctcctg gaccggcgct atggcaatga
     1621 ctgcaacaag cggccctggt ttttcactga tgatggagaa catcgggtat gcggtcatga
     1681 ccgagacccc ctgtgtcatc gtgaacgtcc agcggggagg gccttcaaca gggcagccaa
     1741 ctttatcagc ccagggggac atgctccagt gccgcttcgg ttcacacggt gattatagtg
     1801 tgatcgcact cagcccggcc agtgtccagg agatgtttga tctcacggtc gaggcattta
     1861 atcttgcaga gcgcttcagg agtccggtct ttctcatggc ggacgagatc atcgggcata
     1921 tgagggagcg ggtgatcata ccggaccatg tcccctcgaa tcagagaaaa ccatacgaac
     1981 cgggatccct cccgttcagg gcaggggaag atcttgttcc aggctttccc gcattcggaa
     2041 aaggttatgg agtgcatgtc accggcctca cccatgatga acgaggatat ccctgtgcca
     2101 caagtccaaa actgcatgaa gacctcgtga agcggctggt cgggaaagtc gaaggtgccc
     2161 gtggttcaat aacttcctgc gatatcctta atcccgatgc agaagtggtt tttgtctcct
     2221 atggagcacc atcaaggacc gtacagcagg tgctccatga tcatcccgat gacaatatcg
     2281 gtcatcttcg attaaagatg gtctggcctt ttgccgagat ggttctcgcc cgattcacca
     2341 atgcagaggc tttcatcgta cctgaacaga atctcggcca gatagcccgc gagatcgaac
     2401 gccatactga caaagacgtg gtcacagtcc caaaactggg aggcgatctt catacccctg
     2461 gcgagcttta cgcgattgtg gagaatctca aatgagtgag gaagactggc tcaggcagga
     2521 tcggctccct catatttttt gtgcagggtg tggcaatggc acggtcatca attgtactct
     2581 ttctgctgtc gaacagatag gctggaagag acaagagacg gtatttgtat caggtatcgg
     2641 ctgttcgtcc cgggcacccg ggtacatctc caccgattcg ctccacacga cccatgggcg
     2701 ggcactggct tttgcaaccg ggatcaagct tgccaaccct cagctgaaca tcgttgtttt
     2761 tactggagat ggcgatctgg cagcgattgg cgggaatcat tttatccatg cctgccggag
     2821 gaatatcgat ctcacggtag tctgcctgaa taatatgatc tacgggatga ccggcggtca
     2881 gggaagccct accactccga tgggaggtct ctccaccacc actccctatg gttccggtga
     2941 acctgccttt gatctctgcg gccttgctac tgccgccgga gctaactatg tggctcgctg
     3001 gacgtcatac catgtcaggg agctcaccag ggcaatcctg accggtcttg agaatcccgg
     3061 tttttcattt attgaatcac tggtgcagtg ccctaccaac tatggccgga gaaatcgttt
     3121 caagcaggtt gccgaccaga tcgagtactt caagtcccat accatactcg tcgaaaaggc
     3181 tgccaggatg agagagaagg gggagatgat cccgccagag atgatcctgg caggagagtt
     3241 tgtacggagg aaccggcctg cgctgggggt ccgcccatga ggcacgagat tcgattctcc
     3301 ggctttggcg ggcaggggat catccttgcg gctgtcattc tgggacgtgc agccgtcatg
     3361 tatgataaca agtatgccgt acagacccag gtatacggac ctgaggcgag gggcggagct
     3421 tccatgagtg cagtgatcat cgatgatgaa ccgattctgt atccgaaggt cgcgacaccc
     3481 gatatttacg tcatcatgtc ccaggaaggc ttcgagaagt atggtattgc tgccaccggg
     3541 gaggctcaga tgctcgtgga cagcaccctc gtccattcgc gtccctcctg ccactatatc
     3601 gagatcccgg ccacccggga ggcccgggaa tcactgggca gggatattgt cgcaaatatc
     3661 atcatgctcg gtgccctcgt tgccgctacc gggatcgtga gcaggagcgc tatcgagaaa
     3721 gcaatccttg attcagttcc taaaggaacc gaaggcctga atgtgaaagc gatgacgcgt
     3781 ggctttgaac ttgccgaaga ggcgatcagc agatgaaatt tcttgaacat gaagcaaaat
     3841 cggtcttctc tgaaactgga atccaggttc cgaggggttt tctgatccat tcagcagatg
     3901 aacttgcggg atacctggaa caactgccac ccgaatttgt cctgaaggcg caggtggacg
     3961 tggggggacg cggaaaagcc ggcgggatcc tcatggcaga tcggaagaac ggaattgaga
     4021 atgcaaaagc attgtttgga aagagtatca agggtgttgc ggtaaaggag atccttgcag
     4081 aggagcggct ggagattcag cacgagtact atgcatccat tgcagtcgac cgctctacga
     4141 aggaagccct cctcctgttc accgaagaag gaggtgttga catcgagatc ctggccaggg
     4201 agaacccttc tgcaatcagg agagcgtcac ttcccctcct gatgaccgag atcccgcctt
     4261 tcatgcttcg ggatctgctc gggggagctc ccagagaagt gggaaaggtg ctccagagcc
     4321 tgtatactgc attctgccga aaggatgcca ttcttgccga gataaatcct ctagtggcca
     4381 cccctgcagg cctctttgct gcggatgcca agctgatcgt agatgataac gctctcaggc
     4441 ggcagggaat atcctgcaac cgggatatga ccgatcgcga acgggaggct gagagacacg
     4501 gattttctta tgtcgagctg gagggaaata tcggggttat cgggaatggt gcagggctga
     4561 ccatggcaac actggacctg atcagcttct tcaagggaaa agccgccaac ttccttgatg
     4621 tagggggcgg ggctgatcag gaacgcgtga tgcatgccgt ccggctcgtt gctggtgttc
     4681 cctcagtaaa agtgatcatt gtgaaccttc ttgggggaat cacccgctgt gacgaggtag
     4741 cccgcggaat catcgcagct ggcatcagcc aggaggtctt tgtccggctt gccggaacaa
     4801 atgaggagga ggggaggagt cttctcagcg agcgaggata ccggatggtc ggttcgatgg
     4861 aggaggcggt cagggatgca gtggaggcgg tcagatgatt tacgcagaca aatcggccgg
     4921 gatcattgtt gccggagcca caggacgcca gggtggattc catattgcgc tgatgaatga
     4981 atatgcaaag acggtagggg gggccggagt tgttgcaggg gttacccccg gcaaaggggg
     5041 ccaggtcgca agcggcgttc cggtttacaa cagtatcagg gaggcaatgg cagacatcga
     5101 tgcagaggtg agcgtaattt tcgtccctgc tgctgctgcc ggagactcta tcatggagtc
     5161 agcaaatgcc ggcctttcac tggccgtgac catcaccgaa cacatccctg tccatgatac
     5221 catgaacgca atcgcttttg caaagctcca tgattgtgct gtcattgggc cgaacggccc
     5281 cggcctgctc tctcccggag agatcaagct cgggatcatg ccgtctcatc ttttctcccg
     5341 cggaaatatt ggtgtgatat cgagaagcgg gaccctcacc tatgaaattg tagatgaact
     5401 cacaaaggct ggactcggcc agagcacggt cgtaggaatc ggaggagacc cggtagtcgg
     5461 gcaaaccttt tccgatgttc tccggcgttt tgaggaggac ccgcagacaa aagcagttgt
     5521 tctcatagga gaggtcggcg gggctcttga agaggagggg gcacgctcca cggatctccc
     5581 catcgcggca tatatcgccg gcatctctgc tccacccgaa aagaggatgg ggcatgcagg
     5641 tgcgataatt gaggggggag aaggtgacgc ccgttccaag atagagcggc tccggaagat
     5701 ggaggttccc gttgcgtcac gaccatccga aatccctggg atcatcaaaa aaattctcta
     5761 actcgatttt ttcacaagag agaatacccc tttttcaccc tcatccggag attgaacaat
     5821 gtcattacgg ctggaggatc tcttatcatt atcagtgacg cctcaaaaat ccggaattcc
     5881 gtctcttaag gcgttttatc ctgaaacaat ataaattcaa aaaccaaaaa agaacaggta
     5941 catcagcagg gtggaattat gaacaaaaac agttttgctt ctcctgaccc ggtcgggaag
     6001 gatgaaggta cacctggcgt ggagcggtat ctccatccgg gagagaacct caggatatat
     6061 tcctccgata tccagataaa aaaattcagg tttgaatcat atcttacaaa taaaaggctt
     6121 tttttcatcg atcaaaatga ccgggatctg gggataacgg ccaaagagat tccagtggaa
     6181 tcgattgtaa gcggctatct cgaagaatca acgatcaggg aaccagtact cgtgctaacc
     6241 gttcggacat ctgatgatga tacgaggacc atgaagatgg tatttgtcca tacaggggag
     6301 gatcgtgcgc gggaagctga agactgggtc catctgatat cccaggctgc atcaaccgca
     6361 gttccagtat ctgacagaga aagggaacct gcgatagctc caggagcaag ggcgctgacc
     6421 gacactatca tccttcctgc tcctgggagg tctgaaataa tcagcgagaa gaatgtgcct
     6481 cctcccgcac aatcatctgc cagatcaagt cccgatcagg tttctccaaa accagtacct
     6541 gagcaggggc ggccgacaac ggtatcatca ccttctcccg atctcatcca ttattgttat
     6601 cattgtggaa aaaaactgcc agaaaaagct aacttttgtc cattttgcgg aacgcgggtc
     6661 catgaaacat cccatgaaac cacatcccac cttcgcctgc caattcacca gaccagggac
     6721 cacatggtcc catcccctga agagccgaaa aaaaaagcgg gctggagacg atttttcggt
     6781 cgataaccaa tttttatcat ccatttcctt attttcaacg catacaattt tcaagaggga
     6841 tttctttttt atatgaaaac acgaatgatg caatacggaa aacctgatca agccatgcac
     6901 ccccttgtcc accagagaga gaaaatgttc cggttgttcc tccggttccc tgagaaaatc
     6961 aagcacacta tcgcctggct gcatccctga gtgattattg gcgaatacat gcaaaatttc
     7021 ggcagctcac cctggatttc aaccgccact ttttagcccc cgattatagg aatttaccaa
     7081 ggggggaacc atgctcactc ccacctgcct tccgatgaca tcccttctta tagatccaat
     7141 cctccaggga tacccggcgt taattactga ttcaggtctt ttcattaaaa acgatagtat
     7201 gaatgccgtt cataagaaag gaatgattaa atgatttccc gtcgttttta ttatgattat
     7261 accgctggag aatgaccata agatgaagag aaatatcaga gttgaggcgc tgcatcagat
     7321 tccgctcgaa agacagggga ttgagcttgt cgagaggaaa tgcctgggcc atcccgatag
     7381 tcttgccgac ggggttgcgg aggccatcag tagggctttg tcccttgcat atcttgaaga
     7441 atgtggcgcg gtgctccacc ataataccga ccagggtgag atcgttgccg gcgaatcgat
     7501 accaaaatac ggggggggga aagtcatccg gccgatttat atgcttatcg atggccgcgc
     7561 tacccggcag ttcaatggca tcaatattcc cactgatgca atcgctctgg agacggcgcg
     7621 aaagtacttc aggactaccc ttaccaacct caatcttgag cgcgatatca cgatcgactg
     7681 cagactgggg accggatcca ccgatctccg ggatgtcttc aagccctgcc aggggactgc
     7741 accacgtgcc aatgatacgt cctttggggt gggccatgct ccgttctctg aaacagagac
     7801 cattgtcctc ggactctcag attatatcga tacgaccctt cgggcacgat accccgcaat
     7861 agggcaggat atcaagatca tgggccttcg cgccggagat acgatcaccc tcactattgc
     7921 ttcagcaatg gttgatcgct actgcagcgg cctctcccag tatatcgaat ataaggacat
     7981 tctcaaagag cacgccgaaa aggtggccag gaaaatcacc tccagaaaag tgagcgtcca
     8041 ggtcaatacg gccgatgata tcgatacggg aagtgtgttc ttaacggtaa cgggcacctc
     8101 tgccgagatg ggagatgacg gttccgttgg acgcgggaac cggtgcaacg ggctgatcac
     8161 tccgaatcgt ccgatgagca tggaggcgac gagcggaaaa aatccgatca accacatcgg
     8221 taagatttac aatatccttg cgaccatgct cgcaaaagaa tgtgtaaaag aagttgacgg
     8281 gattgaagag gtctatatcc ggctcctctc gcagataggg catcccatcg accagcctct
     8341 tgttgcgagc atacaggtcc tgccacggga ggaggtccac gtgagtgaaa tcagccggga
     8401 cattgagggt gttattgact cagggcttgc aaacataacc tgcatcactg aaaagatcat
     8461 ccgtggggaa gttaggacct tctgaccaat cagacaagag gagaacaatc ctgtcatcgg
     8521 aacattcatc catggtgcgg cttgcgatac cgaacaaagg acggatagcc gcgccggtaa
     8581 tggaccttat ggagaagagt ggtctccacc tgctcgacag ggaagaacgg aagctgatat
     8641 cgaggacacc tgatccccat atcgaggtgc tttttgcgcg tcccattgac atcccagaat
     8701 acgttgcaaa cggggctgca gatcttggaa ttaccggaag ggacatggtg tgcgaaagac
     8761 aggctgaggt cgatgagctg ctggacctgc gattcgggaa tgccact
//
LOCUS       DAOOCO010000067         8802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7518_length_8802_cov_4.551160, whole genome shotgun sequence.
ACCESSION   DAOOCO010000067 DAOOCO010000000
VERSION     DAOOCO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7518_length_8802_cov_4.551160"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PK154_05150"
     CDS             complement(<1..552)
                     /locus_tag="PK154_05150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPW10482.1"
                     /translation="MNATPYLTPQQIIDRFSARFGNGLSEAKVTERGEGTRKVKAYNL
                     WFRIDREQVRPAIEELIAIRFPHLAVIAGNDLGTDIELLYIFSVFYGQKFGEYMVTIA
                     IRLPKSDLTVPTITDLIPGALFSEREKQEFFGITVTGIPDGRRLFLPEDFPKGVYPWR
                     KDETGIPPEMIKNLWEVDRPKDRP"
     gene            complement(549..980)
                     /locus_tag="PK154_05155"
     CDS             complement(549..980)
                     /locus_tag="PK154_05155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPW10483.1"
                     /translation="MKIPTSFNRSLWVFHLNTGSCNGCEIEIVATITPRYDPERFGIR
                     LVGSPKHADVLLVTGPVTKKMAPRVRRVFEQVPDPKVVMCIGTCGQSGGVFYDSYNLD
                     GPIDQVIPVDVYVPGCPPRPEAIVFGVVKALQKLERLEAEQ"
     gene            complement(982..1326)
                     /locus_tag="PK154_05160"
     CDS             complement(982..1326)
                     /locus_tag="PK154_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPW10484.1"
                     /translation="MVSATSLTSGFGIWDPILWVLAIFIGLVIAWILWSSGVSTYKKG
                     TEQARPYLSGNAEPPKGEVHIRAGNLYWGFTEGIKGYYQRIVPLHTGIATDYLLWIFW
                     VAGILLVIVMVV"
     gene            complement(1329..2903)
                     /locus_tag="PK154_05165"
     CDS             complement(1329..2903)
                     /locus_tag="PK154_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPW10485.1"
                     /translation="MIPGYITTNAPALLISVPLLGAFLSPLIGKISRDARALWVLAVM
                     TLTALIGYFVAFLVYTEGTLLYVFGATNATLAVPPDSGGIPIRIIFTLDAMSALMVTI
                     AVTVGFAVILYSLTSDAKRSGLEAYYTIFLLMIGAILGMVSTGDLFNFFVFLEISSLA
                     SAGLVAYKVEKGRAVEAGLKYLLVSTIGGIFFLFAIGILYGQYNALNMAMLADMIQWT
                     PLDKLALVLVVAALAAKCGAVPMHFWTPDAYSMAPSAVTAMLVVASQASLYGLFRICF
                     TLYGITLNYVTIGWVIIILGILSMIIGVTMAIPQKDIKRLMAYHAVSQTGYMLLGVGV
                     GLAVLGDQALLNSYGMTAMEGGIFHIFNHAMYKGLLFLTAGAIIYQVGTSDLNRMGGL
                     GHRMKWTMIFFIIGALAIAGIPPFNGFASKFLIYESVFAFSPLLSIIAMVVSILTLAS
                     FVKVFHSVFMGPPLPGNEQVREAPRTMLIGMTILAAIVIISGIFPEFFVQNLITPAAH
                     ALTDQAGYISAVLGGA"
     gene            complement(2900..3244)
                     /locus_tag="PK154_05170"
     CDS             complement(2900..3244)
                     /locus_tag="PK154_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPW10486.1"
                     /translation="MFWNLPYIAVVLLSVIGLATILLKRNLIKIFLGVSILTSAVNLF
                     IIALGYREGGIAPIYTYAVSENMVMATPQALTLTSIVIGVATSALMLSFAVIIYKTYG
                     TVNVDEIKELKE"
     gene            complement(3247..3711)
                     /locus_tag="PK154_05175"
     CDS             complement(3247..3711)
                     /locus_tag="PK154_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPW10487.1"
                     /translation="MSKIVRFGADVLFGAIVIFGLYVIMHGHLTPGGGFQGGAVVATG
                     AALVIVAYSYEEVIGMIKKTILMAQESAGLVMFAGVALIAIGLGTTFFFNFLANSGSL
                     FGMTVPFGPNPGELNTGGVLPLMNIAVGIEVWGGLAIVILYMLSGIGKTKEA"
     gene            complement(3704..3988)
                     /locus_tag="PK154_05180"
     CDS             complement(3704..3988)
                     /locus_tag="PK154_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10488.1"
                     /translation="MRKELSILIIIIFGLFLGLIALDLTFGEPDNTTMDDYFIMNGQE
                     QAATNNIVTTIVFDYRGFDTLGESTVLFTAVVGVALMYRKLVRGDADENE"
     gene            complement(3985..4239)
                     /locus_tag="PK154_05185"
     CDS             complement(3985..4239)
                     /locus_tag="PK154_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPW10489.1"
                     /translation="MIETIIHILVIAGLIVSAVLVYHFKDLLAAALASGLFSFLISLE
                     FYILQAPDVAISQAAIGAGLTTAIFIIAIRSTKRCEEDAP"
     gene            complement(4236..4592)
                     /gene="mnhG"
                     /locus_tag="PK154_05190"
     CDS             complement(4236..4592)
                     /gene="mnhG"
                     /locus_tag="PK154_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPW10490.1"
                     /translation="MIAEWLIIICLAIGIIFNSLGVIGLLRFPDLYTRMHATTKATTF
                     GSVFTTLAVIVYSISLYLSTIDSQFIVLGVHAFIAAFALAFTNAIGSHAIARAAHRSG
                     ILPSPAVVDRLKEAGR"
     gene            complement(4592..4849)
                     /locus_tag="PK154_05195"
     CDS             complement(4592..4849)
                     /locus_tag="PK154_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPW10491.1"
                     /translation="MIDPFYPAVLVLVILICLCFIRLILGPTPGDRVVAVDTVNTLTV
                     ASLVILGLIYREVIFIDVAIVYALLSFVSTLYIAKYLGGEL"
     gene            complement(4846..5373)
                     /locus_tag="PK154_05200"
     CDS             complement(4846..5373)
                     /locus_tag="PK154_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPW10492.1"
                     /translation="MIAFILTFLLSLFVYLVLTAGSGNIGLWSASELTAGVVLSLLTA
                     AVARNFLCRARNYRMANPLRFLLIPIYVFGPFFLEVIKANLDVAYRVITLRIRPGIIR
                     VSSDLKSDLGIFMLANSITLTPGTITVDIDEHTHDLFIHNINVGEGEEAAPVIRDHEL
                     FSIADLPRWIRRIAE"
     gene            5701..7110
                     /locus_tag="PK154_05205"
     CDS             5701..7110
                     /locus_tag="PK154_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HPW10493.1"
                     /translation="MRPSFLGKMLLHWCDICHAPVLSETCACGAVTRKVQVTPPGDIR
                     PAFPADVSLINRIYSAHFGAPLIPEGHVVLLNKVPDTDRMEEIIMGGAVVGAIRYNPS
                     DRAWEPLPRQESALYLTPLLRFVVVDPGAVASIRNEGASVLAPGLISIHPSVRSADEV
                     FILDPEGRVAGVGRARADAIDAASMTRGQVVRTRKNARSTCIPGPSTWDAVVGANAKV
                     LEEAEAEAIGFIRNVSSNYAGQATVSYSGGKDSLATLILVLKAIGNVPLLFADTGFEF
                     PETYQNVSDVAAQYGLPVVRSDGKSRFWEAYDRHGPPAVNHRWCCSACKLLPLKETIG
                     ETWGGCLSFIGQRRYESFRRKESSRVFRNGIVTNQTSAAPIQNWTALHVWLYLFREDA
                     PFNPLYAKRLDRIGCYLCPSSDVAVLEDIKKSYPDLWDTWELKLKTWREDHGLPAAWI
                     DEMRWRIKEGVADEADSNC"
     gene            7088..7693
                     /gene="hisH"
                     /locus_tag="PK154_05210"
     CDS             7088..7693
                     /gene="hisH"
                     /locus_tag="PK154_05210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW10494.1"
                     /translation="MKRIVIVDYGLGNLRSVYRGLEKSGARPVVTFDIEEIAAADAIV
                     LPGVGAFRDGMEQIGEMQQVLTEAAGAVPMLGICLGMQMLLEWSEEHGLHAGLGIIPG
                     TVRRFQGSPGYKIPHMGWNSLESPMTDHPLMEGIREGSYVYFVHSYYADTRAENTLTS
                     TTYTRSFASSITSGNVFGVQFHPEKSGATGLRILENFISLI"
     gene            7774..8571
                     /locus_tag="PK154_05215"
     CDS             7774..8571
                     /locus_tag="PK154_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10495.1"
                     /translation="MKKRFIVLLLSLALLIGAVQAYVVTIEAPPEIQAGAPLEVTGTT
                     TFPAGTQFDIILYRTQFTTPETVDRRMIVVDETKEFDASFPTTALPAGQYKLEVQFLE
                     DPGSSLGSSSVTLLLVNLIDRSAEIVLTAPKEQTLDQALLIEGYIPDLGVATITMKVD
                     GPKGFSLPDQSIRTTTTLGSKDGHFSKKVNVTEPGNYYVNVYDAKGFITQLRYSVSES
                     TSPAGTNPSTTEIPSTSLPQTAGMPVPLAGMIAGLGVAAALVSWKRS"
BASE COUNT         2069 a         2374 c         2494 g         1865 t
ORIGIN      
        1 cggccggtct ttcggccggt ccacctccca gaggttcttg atcatctcgg gaggaatacc
       61 ggtctcatcc tttctccagg gatagacccc cttcgggaag tcctcgggta aaaagagcct
      121 tctcccatct ggaattcccg tgaccgtgat gccaaagaac tcctgcttct cccgctcaga
      181 gaagagagct cccgggatca ggtcggtgat tgttggcact gtcagatcgc tcttcggcag
      241 ccggatggcg atcgtgacca tatactctcc gaacttctgc ccgtaaaaga ccgagaagat
      301 atagaggagc tcgatatcag ttcccaggtc atttcctgcg atgaccgcaa gatgggggaa
      361 ccgtatcgcg atcagctcct cgatggccgg cctgacctgt tcccggtcaa ttctgaacca
      421 gaggttgtat gccttgacct tcctggtccc ttcgccccgt tcggtcactt ttgcctcgga
      481 aagtccgttc ccgaatctcg cgctgaaccg atcgatgatc tgttgcgggg taaggtaagg
      541 ggttgcattc attgttctgc ctccagccgt tccagttttt ggagtgcctt cacaactccg
      601 aagacgattg cctctggccg gggcgggcat cccggaacat agacatcgac cgggatcacc
      661 tggtcgatgg ggccgtccag gttatatgag tcatagaaaa ctccccctga ctggccacag
      721 gtgccgatgc acatcacgac tttcgggtcc gggacctgtt cgaacacccg ccgcactctc
      781 ggggccatct tcttcgtgac aggcccggtc acgaggagca catcggcatg ctttggtgat
      841 ccgacaaggc gtatcccaaa ccgttccgga tcgtaacggg gggtaatcgt ggccacgatc
      901 tcgatctcgc acccgttgca ggaccctgta ttgaggtgga agacccagag cgaccggttg
      961 aatgatgtag gaatcttcat gtcagaccac catcacgatg acgaggagga ttccggcgac
     1021 ccagaatatc cagaggaggt aatccgtcgc gattccggta tgcagcggga caattcgctg
     1081 atagtacccc tttatccctt ccgtgaatcc ccagtagagg ttgcctgccc tgatgtggac
     1141 ctctcccttg gggggttcgg catttcctga aagatatggt cgtgcctgtt cagttccttt
     1201 cttgtacgtg gagacgcctg aactccagag gatccaggct atgacaaggc cgataaagat
     1261 ggctagcacc cacaagatcg gatcccagat cccgaatccg ctggtaaggg atgttgcaga
     1321 taccatgatc atgcccctcc gagcactgct gaaatgtatc ctgcctggtc ggtgagcgca
     1381 tgagcagctg gggttatcag gttctggaca aagaattccg gaaagatccc ggagatgatc
     1441 actattgcgg caaggatggt cattccgatg agcatcgtac gcggtgcttc tctgacctgc
     1501 tcattcccgg gaaggggagg gcccatgaag acagaatgga acacttttac aaacgaggca
     1561 agggtaagga tactcacgac catcgctatg attgaaagga ggggactgaa ggcaaacacg
     1621 gactcgtata tcaggaattt ggaggcaaat ccgttaaagg gtggtatccc ggcaatggca
     1681 agcgccccga taataaagaa gatcatcgtc catttcatcc ggtggccgag acctcccatc
     1741 cggttcaggt cgcttgtccc cacctggtag atgatggcgc cagccgtcag gaacaggagg
     1801 cccttataca ttgcatggtt gaagatgtgg aagatcccgc cttccatcgc agtcatcccg
     1861 taggagttga ggagcgcctg gtcgccgagg acggcaagtc ctacgccaac cccaaggagc
     1921 atgtatccag tctgggagac ggcatggtag gccatcagcc tcttgatatc cttctggggg
     1981 attgccatcg tgactcctat gatcatcgag aggataccca ggatgatgat cacccacccg
     2041 atcgttacgt agttgagcgt gatgccgtac agggtgaagc agatccggaa cagcccgtac
     2101 aggcttgcct ggcttgcaac caccagcatt gccgtcaccg cagagggggc cattgaatag
     2161 gcatccggtg tccagaaatg catcgggacc gctccgcatt ttgctgcaag ggctgcgaca
     2221 accagcacga gagcgagttt gtcgagaggt gtccattgga tcatgtccgc aagcatcgcc
     2281 atgttcaggg cgttatactg gccatacagg atgccgatgg cgaagaggaa gaatataccc
     2341 ccgattgtac tgacaagcag gtatttgaga cctgcctcaa cggcacgacc tttttccacc
     2401 ttgtaggcaa caagccctgc cgatgcgagg gaggagatct cgaggaagac aaagaaattg
     2461 aaaaggtctc cggttgagac catcccgagg atggcaccga tcatcaggag aaagatggtg
     2521 taataggctt cgagcccgga cctcttggca tcgctggtga gggagtagag gataacggca
     2581 aaccccaccg taacggcaat ggtcaccatc agggcactca ttgcatcgag ggtgaagatg
     2641 atccggattg gtatcccgcc agaatcaggg ggaacggcaa gggtggcgtt cgttgcacca
     2701 aagacataga gcagcgtccc ctccgtatat acaaggaacg ctacaaagta accgataagt
     2761 gccgtcaggg tcatgacagc aagtacccag agtgcccgtg catcccgtga tatctttccg
     2821 atcagaggtg agaggaatgc cccgagcaag gggactgaga tcagcagagc cggtgcattg
     2881 gtagtaatat atccagggat cattctttca gctcctttat ctcatcgaca ttcacagtcc
     2941 cgtaggtctt gtagatgatc acggcaaacg agagcatcag tgcactcgtt gcaaccccga
     3001 tcacgatcga tgtgagagtc agagcctgcg gggttgccat caccatgttc tcagatactg
     3061 cgtaggtgta gatcggtgcg atccctccct cacgatatcc gagggcaata atgaagaggt
     3121 tcactgccga ggttaggatg ctcaccccga ggaatatctt gatgaggttt ctcttcagga
     3181 ggatggtggc aagcccgatc acggacagga gtacgaccgc gatataggga aggttccaga
     3241 acatggtcag gcctccttgg tcttcccgat gcccgagagc atgtacagga tgacgatggc
     3301 gagaccgccc cagacctcga tcccgacagc gatgttcatc agcgggagta ccccgcccgt
     3361 attcagctca ccggggttcg ggccgaaggg gacggtcatc ccgaagagtg agccgctgtt
     3421 tgcgaggaaa ttgaaaaaga acgtggtccc aagcccgatg gcaatgagtg ccaccccggc
     3481 aaacattacg agccccgcgg actcctgggc catgagtatt gtctttttga tcatcccgat
     3541 cacctcttca tacgaatagg caacgatcac gagtgccgcc ccggttgcga ccacagcccc
     3601 accctggaat ccgcctcccg gggtgagatg accgtgcata atcacgtaaa ggccgaagat
     3661 aacgatcgca ccaaagagga catctgcccc gaagcggacg atcttactca ttctcatcgg
     3721 catcacccct taccagcttc cggtacatca gcgctacccc gacaacggcg gtgaacagga
     3781 cggtggattc accgagcgtg tcgaaaccac ggtaatcgaa gacgatcgtg gtcacgatgt
     3841 tgttcgttgc agcctgctcc tgcccgttca ttatgaagta atcgtccatc gtggtgttgt
     3901 caggttcccc gaatgtcagg tcgagggcga tcaggcccag gaagaggccg aaaatgatga
     3961 tgatcaggat cgagagttcc tttctcatgg cgcatcctcc tcgcagcgtt tggtcgagcg
     4021 gattgcaatg ataaagatag ccgtagtaag acctgcccct attgccgcct gtgagatcgc
     4081 gacatcgggc gcctggagga tatagaattc aagcgagatg aggaaactga agaggcccga
     4141 ggcgagtgcg gcggcgagca gatccttgaa gtggtacacg agaactgccg agacaataag
     4201 gccggcaata acgaggatat gaatgatagt ctcgatcatc gtcccgcctc cttcagccgg
     4261 tcgaccactg caggggaagg caggatgccg ctccggtgag cagccctcgc gatggcatgg
     4321 gagccgatag cgtttgtgaa tgccagggca aatgcggcga taaaggcatg gacgccgagc
     4381 acaatgaact gcgaatcgat ggtgctcagg taaagggaga ttgaatagac gatcacggca
     4441 agggtagtga agaccgaccc gaaggtggtt gctttcgtgg tggcatgcat ccttgtgtac
     4501 aggtccggga accgcagcag gccgatcacc ccaagcgaat tgaagatgat cccgatcgca
     4561 aggcagatga tgatgagcca ctcggcaatc atcagagttc ccctccaagg tatttcgcaa
     4621 tgtagagggt gctgacaaac gagagcagtg catagacgat tgccacatcg ataaagatga
     4681 cctcccggta gatcaggccg aggataacga gcgatgcgac ggtcagggta ttgaccgtgt
     4741 cgacagcgac gacccggtca ccaggggtag ggccgaggat aagcctgatg aagcagaggc
     4801 agatcaggat cacaaggacc agaactgcag ggtaaaacgg atcgatcatt cggcaatcct
     4861 ccggatccac cgcgggagat cagcgatgga gaagagctca tggtcacgga tgaccggtgc
     4921 agcctcctca ccctctccga cattgatgtt gtggatgaac agatcatgcg tatgctcatc
     4981 gatgtcgacg gtgatcgtcc caggtgtaag ggtgatagag tttgcgagca tgaagatccc
     5041 gaggtctgac ttcaggtctg aggagaccct gatgatccct ggccggatcc gcagggtgat
     5101 cacccggtag gcaacatcga ggtttgcctt gatcacttcc aggaagaacg gcccaaagac
     5161 atagatcggg atgagcagga accgcagggg gtttgccatc cggtaatttc gtgcccggca
     5221 gaggaagttc cgggcaactg cagcggttaa aaggctgaga acaacccctg cagtgagttc
     5281 tgatgccgac cagagcccta tattcccact ccctgcagtc aggacaaggt agacaaagag
     5341 ggagaggaga aaggtgagga taaaggcgat cacccgatcg cctccggccg ttcatcgttt
     5401 gagcatgtca aacacgttcc attcataggt atcacgcgaa tctccagagg ttccggtgcg
     5461 gtcctgatac agctttcatt ggtgaacacc gatctgccgc atcacatggt ataactacga
     5521 atattggttc tccgaatctt aaacaatacg atttgctccg gataaaaaac gcattttatc
     5581 agttcttgga aaaaattacg tctaattatc agtttttacc cactacaccg ggatcccggc
     5641 aaaaatatat cgcccgtatc cctctggtat ttatcgtttc attgccaatg gtaagggtga
     5701 atgcgccctt cgttcctggg aaaaatgctc cttcactggt gcgatatctg ccacgcacca
     5761 gtcctctcgg agacctgtgc ctgtggtgcc gtgacacgga aagtacaggt cacccctcca
     5821 ggtgatatac ggcctgcgtt tcctgcggat gtctccctta tcaaccgtat ctattctgcc
     5881 catttcgggg cgccactcat acccgaaggg catgtcgttc tccttaacaa ggttccggac
     5941 actgaccgta tggaagagat catcatggga ggggccgtcg tcggggcaat acggtacaac
     6001 ccgtctgaca gggcctggga gcccctcccc aggcaggagt ctgcccttta cctgaccccg
     6061 ctgctccggt ttgtggtcgt ggatcccggt gcggttgcct cgattcggaa tgaaggggct
     6121 agcgtgcttg cacccgggct tatctcaata cacccctcgg tccggtcggc tgatgaggtg
     6181 ttcatccttg atcccgaagg aagggttgcg ggagtcggga gagcccgtgc tgatgcaatt
     6241 gatgcggcct cgatgacccg ggggcaggtg gtgagaaccc ggaagaatgc ccggtcgacc
     6301 tgcatcccag gtccatccac gtgggatgcg gtggtcgggg cgaacgcgaa ggtgcttgag
     6361 gaggccgagg cagaagcaat cggtttcatc cggaacgtct cgtcgaatta cgctgggcag
     6421 gcgaccgtat cgtactcggg cgggaaagac agcctcgcca ccctcatcct tgtgctgaag
     6481 gcgataggca acgtcccgct cctgtttgcc gataccgggt tcgaattccc ggaaacgtat
     6541 cagaatgtct ctgatgtggc agcccagtac gggcttccgg tcgtccgttc cgatggtaaa
     6601 agcaggttct gggaggcata cgaccggcat ggtcctcctg cagttaatca ccgctggtgc
     6661 tgcagcgcgt gcaaactcct cccgctaaaa gagacgatcg gagaaacatg gggtggctgt
     6721 ctctcgttta tcggccagag aaggtatgaa tcgttcagga ggaaggagag cagccgtgtg
     6781 ttcaggaacg ggatcgtgac caaccagacc tcggctgccc ctatccagaa ctggaccgcc
     6841 ctccatgtct ggctgtatct cttcagggaa gatgccccgt ttaatccgtt gtatgcgaaa
     6901 cgcctcgatc gtatcggatg ctacctgtgc ccttcaagcg atgtggccgt gctcgaggat
     6961 atcaagaaga gttatcccga tctctgggat acgtgggaac ttaagctcaa aacgtggcgt
     7021 gaggatcatg gcctgcccgc tgcatggatc gatgaaatga ggtggaggat aaaggagggg
     7081 gtggcagatg aagcggatag taattgttga ttacgggctt ggaaacctaa ggagtgtcta
     7141 ccgggggctt gaaaaatcag gagcacgacc ggtcgtcacc ttcgatatcg aggagattgc
     7201 ggcggcagat gccattgtcc ttcccggcgt tggtgccttc agggacggca tggagcagat
     7261 cggcgagatg cagcaggtcc tcaccgaggc agccggggcc gtccctatgc taggcatctg
     7321 cctcggtatg cagatgctcc ttgaatggag cgaggagcat ggtctccacg cagggcttgg
     7381 gatcatcccc gggacggtcc ggcggttcca gggatcaccc gggtataaaa ttccccacat
     7441 gggatggaat tcgctcgagt ccccgatgac tgaccacccg ctcatggaag ggatcaggga
     7501 aggttcctac gtctactttg tccattcgta ttatgccgat acccgcgctg aaaatactct
     7561 cacgtccacc acctacaccc gctcctttgc ttcatcaatc acgagcggaa atgtctttgg
     7621 tgtccagttc cacccggaaa agagcggggc gacggggctc cggatccttg aaaacttcat
     7681 cagtctcata tgagatgatg cacggggaag accggccctg attctgttcc agagaacaga
     7741 aactaattat tacccacgct ccaagagctg gatatgaaaa agcgctttat agttcttctt
     7801 ctctctctcg cgcttcttat cggggctgtt caagcctatg tcgttacgat cgaggcacct
     7861 ccggaaatcc aggcaggggc tccccttgaa gtgaccggta ctaccacgtt cccggccggg
     7921 acccagtttg acattatcct ctaccggaca cagttcacga cacccgagac agttgaccgg
     7981 agaatgatcg tcgtcgatga gaccaaggag ttcgatgctt catttccaac aactgccctc
     8041 cctgccgggc agtacaagct ggaggtccag ttcctcgagg atccggggtc aagcctgggg
     8101 tcgtcatcgg tcaccctgct tcttgtgaat ctcatagaca ggtctgcgga gatcgtcctc
     8161 accgctccaa aggagcagac cctggaccag gccttgctga tcgagggata tatccctgat
     8221 ctgggggttg ccaccatcac gatgaaggtc gatggcccca agggtttctc cctgcctgat
     8281 cagagcatca ggacgaccac tacccttgga tccaaggacg gacacttctc aaagaaggtc
     8341 aacgtgaccg aaccgggaaa ctattacgtg aatgtctatg atgcaaaggg cttcatcacc
     8401 caactgcgat actcggtaag tgagtcaacc agcccggcgg gaacaaaccc atcgaccacg
     8461 gaaataccat ccacatccct cccccagaca gcgggaatgc ccgttccgct tgctggaatg
     8521 atcgccggcc tcggcgtagc tgccgccctg gtctcctgga agaggagcta attttcaccg
     8581 gaaccggagt agtatctcct ttttctcccg taatccagct taatcttcac cagtgggaca
     8641 gatagccatg gccatttttt ccctgcaatc acaactgaag ggggaaaaaa gggtgatgag
     8701 gagcagatca cccgaaatct cgtggaattg agcatataac gtaaagaagg gtacgcacaa
     8761 tttttaccac ctgtctttct cataccctat gacagaaaat tg
//
LOCUS       DAOOCO010000068         8568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7777_length_8568_cov_4.068836, whole genome shotgun sequence.
ACCESSION   DAOOCO010000068 DAOOCO010000000
VERSION     DAOOCO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_8568_cov_4.068836"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK154_05220"
     CDS             complement(<1..634)
                     /locus_tag="PK154_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAS-barrel domain-containing protein"
                     /protein_id="HPW10496.1"
                     /translation="MNRVPLFTKEARAISYVSGPLIFIQNVKGVSYGEIVRIILPTGE
                     ERTGQVLDISKDIAVTQVYEGTSGIDNKETRVRFTGEPPKVKVSIEMLGRIFNGVGKP
                     RDGGPEIIEEANLDINGMPINPSARDKPADFIQTGMSTIDGLNTLVRGQKLPIFSGAG
                     LPANKLAAQIARQAKVLGEEEKFAVIFVAMGITYKEASFFMRDFEKTGALE"
     gene            complement(631..2370)
                     /locus_tag="PK154_05225"
     CDS             complement(631..2370)
                     /locus_tag="PK154_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPW10497.1"
                     /translation="MIIGGISRISGPVVLSEGMRGSRMYDIVKVGEQKLNGEIIRLDG
                     DQAVIQVYEDTSGLQVGEKVTNTGLPLSVELGPGLLSSIYDGIQRPLPLLAEKSGSFI
                     SRGVTLPGLSHETKWEFKPQVKKGDHVSGGDVIGTVTEYHLEHRVMMPPFVSGTVQDI
                     HGGNFTIDEPVCTLENAQQIPMLQTWPVRKGRPYQRKMDPDTLLVTGQRVFDTLFPVT
                     KGGTAMIPGGFGTGKTVAEQTLAKWSDTQIVVYIGCGERGNEMTDVLTEFPELLDPRT
                     KRPLIQRTILIANTSNMPVAAREASIYTGITLAEYYRDMGYDVALMADSTSRWGEALR
                     EVSGRLEEMPGEEGYPAYLATRLAAFYERAGRVVCLGSDERKGSVTVVGAVSPPGGDF
                     SEPITQNTLRIAGTFWALDTSLAYRRHFPSVNWIKSYSLYLDQIKEWFATSGFSDWKD
                     LRARAMYLLQKEVELQEIVQLVGPDALPESEKAILEITRMIREDFLQQSAYHDVDSFC
                     SLEKQYWMLRVILSFYDRLDLAMGKGVPLAKLLKLPVKIEIGRMKELTDIEKIKDLIT
                     QFDGAIGKLEAER"
     gene            complement(2375..2695)
                     /locus_tag="PK154_05230"
     CDS             complement(2375..2695)
                     /locus_tag="PK154_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPW10498.1"
                     /translation="MYKFIVVTDPDTAPGFRLAGIDVMEIQNSEEVRVLLPSLLLKDD
                     TGIIAINEDYMMSLDEKMMEKIEKSYRPIIIPIPSRAKRLDRTSYIEGLLRRAIGYNI
                     VLRK"
     gene            complement(2688..3740)
                     /gene="ahaC"
                     /locus_tag="PK154_05235"
     CDS             complement(2688..3740)
                     /gene="ahaC"
                     /locus_tag="PK154_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase A1 subunit C"
                     /protein_id="HPW10499.1"
                     /translation="MEYAYVNARIKAMKSRLLDPSLLEQLINKPDVDSLISELEKTTY
                     REELEKAGVLYSGINRIEVAIRKDLVKTFRTILSLIKGGETETYLTIILNRWDVQNIK
                     TIIRGKKIQENPAEILECLIPAGELDEAALTELVKQPDVKAVIDLLATWGIAYARPLT
                     HNFKEFTDSGDMLVLEYALDTFYYQDAMSKLKTAESDDARILKNILVTEIDINNIKTV
                     LRSVRDRIDIDEAKRYFVQGSPDFDTEKLLSMMKAGTIEGVLKQLEHTPYQFIAKIPP
                     EYIAAEKISAFEKELERYLVKKAVGQFMGDPLSIAIPIAYIWAKFTEVTNIRVIARCK
                     VTEVPEKELREALISV"
     gene            complement(3742..4326)
                     /locus_tag="PK154_05240"
     CDS             complement(3742..4326)
                     /locus_tag="PK154_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPW10500.1"
                     /translation="MAIDDLIKAVEVSGQERILEIQERSKAEADEIIKDAQAKDQPIK
                     KRLMEEATQSVAIQRNKILSEVREKSRMELIKAKNETFERVFDEAVRNLESSREHSRY
                     KEILNSLLKEAIGDLGSDGVIFHIDKRDEALLKDILNEMKISGEIITDLTSAGGLNAY
                     SHDGRFVIFNTLESRLKKAKEIYRPEIFSLLFGE"
     gene            complement(4397..4615)
                     /locus_tag="PK154_05245"
     CDS             complement(4397..4615)
                     /locus_tag="PK154_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW10501.1"
                     /translation="MVDFGIPIGAGIAFGLGALGTGIAQSRIGAAGAGTIAEKPEMFG
                     LMIILVAIPETLVILGFVVASMIMIMLV"
     gene            complement(4812..6752)
                     /locus_tag="PK154_05250"
     CDS             complement(4812..6752)
                     /locus_tag="PK154_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPW10502.1"
                     /translation="MVKIQVLGPKKDLPNVLDVLYAKGTVHIEDASVPVTPGEITLKK
                     MTAEQVGDIAVAHSKIGGILLTLPKIKTDEKAVAYSYSSLKAMSHESLVARANTVISE
                     LEMATKTLAKEKSDLELALINLGRYEKIIDKIRPLETSMPKLEGFEVTVLLIQKEFKD
                     VIDIIRNALAEITKRQFELMSAELDETTIAAVTIFNKRYSEQVHSYFFNQNVNEVRLP
                     PEYLGKPFNEIFNLIEERKTSSTQEISRLNAELEKISSTSYTELSALHRILGERTEEL
                     SIFSKIGQTEYTFVLAGWVPKKALKEMKNALKDTFEGRVIVHELVLTPNEMAEAPTFY
                     DNPSFVKPFEFLMRVVSPAKSTEFDPSPIMAIFLPLFFGIMVGDIGYGLIILAFAIIM
                     KWRFADRDWLQHLMNVLIMGALWSIFFGWLYGEFFGDLGEHMGWIEPKTFFGVTWNRM
                     EAIVPFLILSIAIGVFHVFLGLILGIVNSYTELSCKRHVDKAKKHICEKSGMIIVITG
                     LILVIAGVAMTLPTALITTGAVLIIIAIPLIIIGGGAFGTFEIMSTVTNILSYSRIMA
                     IGMASVILAVVANKLGGMMEVAIVGIIIAVLLHTLNLVLAMFSPFLHSLRLHLVEFDS
                     KFYEGGGKLYKPFKKEEEGKSV"
     gene            complement(6771..7091)
                     /locus_tag="PK154_05255"
     CDS             complement(6771..7091)
                     /locus_tag="PK154_05255"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HPW10503.1"
                     /translation="MPVEKSLLAKIREKELEVSVKIDDARVGADHMIDRARKDSLEII
                     TNSEKEGKKAADDFLESEMKLVQAEADQIRTKARNEVESVRQRGENNLPRAVERITSI
                     ILSG"
     gene            7527..8450
                     /locus_tag="PK154_05260"
     CDS             7527..8450
                     /locus_tag="PK154_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW10504.1"
                     /translation="MFHKILFATDFSEYANKTLDCIAGLPGAREIILLHVLEEARNPR
                     GGGEIVETLTPDERNRLKEAKHRLESLNKSLKVVTRVKTSSDIPGAIIEAAEENGVTL
                     IVVGARGNSIVEGILLGSVSVAIVRRSKITVLVMRHKIVAGLAGRAYEMWCPMILSRI
                     LCPIDFSVCSDRAITLLKTTKGVGQVILLHVVSHGESESELETTVSEAGVKLAEIGRD
                     LSQAGVNVRTLVKRGNPGSEITRIAEEEDVSVIWISSLGKGWFRELLLGSTAYDVAMN
                     SKQPVIIVRTTKSKRENQALSENNQYRYDVA"
BASE COUNT         2028 a         2195 c         2009 g         2336 t
ORIGIN      
        1 gttcaagggc cccggtcttt tcgaaatccc gcatgaagaa cgaagcttcc ttataggtaa
       61 ttcccatcgc tacgaaaatt actgcaaatt tctcttcctc cccaagaacc ttcgcctgtc
      121 ttgcaatctg tgcggcaagc ttgttagcgg ggagacctgc accagagaag ataggcagtt
      181 tctgaccccg gacaagggta ttcagaccat caatggttga catcccggtc tgaatgaaat
      241 cggcaggttt gtcacgggct gacggattga taggcattcc gtttatgtcg aggtttgcct
      301 cttcaatgat ttcaggtcct ccatcacgcg gttttcccac accattgaat atcctcccca
      361 gcatttcgat ggataccttg actttcgggg gctctccagt aaagcggact cgtgtctcct
      421 tgttatcgat cccactcgtg ccttcgtata cttgggttac tgcgatatct ttcgaaatat
      481 ccagtacctg cccggtgcgt tcctctccgg tgggaaggat gatccgaaca atttcaccat
      541 atgagacacc tttgacgttt tggatgaata tcaacgggcc tgaaacatag ctgattgctc
      601 tggcctcttt ggtaaagagt gggactctgt tcatcgctct gcctccagct taccgatcgc
      661 tccatcaaac tgggtgatga ggtccttgat tttttcgata tcggtgagct ccttcatccg
      721 tccaatctca attttcacgg ggagtttaag gagtttggca agcgggactc cttttcccat
      781 cgctagatcc agccggtcat agaaggagag gatcacccgg agcatccagt attgtttttc
      841 gagagagcag aaggaatcga cgtcatgata agcactctgc tggaggaagt cttcgcgtat
      901 catacgggta atctcaagaa tcgccttttc actttcgggg agggcatccg ggccgacgag
      961 ttgcacaatc tcctgcagtt caacctcttt ctggagaagg tacattgcac gtgctctgag
     1021 atctttccag tcagaaaaac cgcttgtggc aaaccattct ttgatctggt ccagatagag
     1081 ggaatagctt ttaatccagt tcacggaggg gaagtgcctg cggtatgcaa gactcgtatc
     1141 gagagcccag aaagttcctg cgatacgcag ggtattctgg gtaatcggct ccgagaagtc
     1201 tcctcccggc ggtgagacag cgccaaccac cgttaccgat ccctttcgct catcagaacc
     1261 gaggcagacc acgcgacctg cccgctcgta gaatgctgcg agacgtgttg caagatatgc
     1321 agggtatcct tcttcacccg gcatctcttc gagccggcct gaaacctcac gcaatgcctc
     1381 gccccaccgt gaagtagagt ctgccataag tgcgacatcg taccccatgt cgcggtagta
     1441 ttcggcaagg gtgatcccgg tatagatgga ggcttcacgg gctgcaaccg gcatgttgga
     1501 ggtgttagcg atcagaattg tccgctgtat cagggggcgt tttgtcctcg gatcgaggag
     1561 ttccgggaac tcggtgagaa catcggtcat ctcgttgccc cgctccccgc atccgatata
     1621 aacaacaatc tgggtatcgg accatttggc aagcgtctgt tctgcgacgg tttttccggt
     1681 tccgaatcca ccgggaatca tcgcagtccc tccctttgtc acggggaata gtgtatcaaa
     1741 taccctttgg ccggtaacga ggagggtatc gggatccatc ttcctctgat agggtctgcc
     1801 ctttcttacc ggccaggtct ggagcatggg gatctgttga gcgttttcga gggtgcaaac
     1861 cggttcgtcg atggtaaaat ttccgccatg aatgtcctgt accgtaccgg aaacaaatgg
     1921 cggcatcatg actcggtgtt cgagatgata ctctgttacc gtcccgatga catccccacc
     1981 agatacatgg tcgcccttct tcacctgtgg tttaaactcc cacttcgttt cgtgagagag
     2041 tcctggcagg gttactcccc tcgagataaa acttccgctc ttctctgcaa gaagggggag
     2101 tggtcgttga ataccgtcat agatggagga gagaagaccc ggtccaagct cgaccgagag
     2161 tggcagcccg gtattggtga ccttctcgcc tacctgcagg ccggatgtgt cctcgtacac
     2221 ctggattacg gcctgatcac catcaagccg aatgatctcg ccattcagtt tttgctcccc
     2281 gaccttcact atatcataca tccgagatcc cctcatccct tcagagagga caacagggcc
     2341 cgaaattctg gatataccac caataatcat acagtcactt cctcagcacg atattgtacc
     2401 cgattgctct tctcagcagc ccctcgatgt aactggtccg gtcgagtctc tttgctctgg
     2461 aagggattgg gatgatgata ggccggtagg atttctcgat cttttccatc atcttctcgt
     2521 ccagtgacat catgtaatcc tcgttgatcg ctataatgcc agtatcgtct ttcaggagaa
     2581 gagaagggag caacacacga acctcctcgg aattctggat ctccatcaca tcgatgccgg
     2641 cgagccggaa tccgggagca gtgtcaggat cggttaccac tatgaactta tacactgatg
     2701 agcgcctccc tgagctcttt ttctggtact tctgtgacct tacagcgggc aataacccgg
     2761 atattcgtaa cctcggtaaa ttttgcccag atataggcaa tcggtatggc gatagagagt
     2821 ggatctccca tgaactgacc tactgccttt tttacaagat atcgttccag ttccttctca
     2881 aaggcagata tcttctctgc cgcaatgtat tcgggaggga tctttgcgat gaactgatag
     2941 ggggtatgct cgagctgttt cagcacaccc tcgatcgtcc ccgctttcat catggaaagt
     3001 agtttttcgg tatcaaagtc agggcttccc tgcacaaaat accttttcgc ctcgtctatg
     3061 tcgatcctat cacgtaccga acgtagcact gtttttatgt tgttgatatc aatctcggtg
     3121 acaaggatat tcttgagtat ccttgcgtca tcggactcag cggttttcag ctttgacatc
     3181 gcgtcctgat agtaaaaggt gtcgagtgca tattccagta ccagcatgtc ccctgaatca
     3241 gtgaattcct tgaaattgtg ggtgaggggg cgtgcatacg caatacccca ggttgcgagg
     3301 agatcgatta ctgccttgac atcgggttgt ttcaccagct cggtgagagc tgcctcatca
     3361 agttctccgg ctgggatcag acactcgagt atttcagcag gattttcctg tatttttttc
     3421 cccctgatga tggtcttgat attttggaca tcccagcggt tgaggatgat agtgaggtag
     3481 gtctccgttt cacccccctt gataagcgag aggatagtcc tgaacgtttt aacgaggtct
     3541 tttcgtatcg cgacttcaat gcgattgatc ccggaataca gcaccccggc cttttccagt
     3601 tcttccctgt acgtggtttt ttcaagctct gatatcaggg aatcgacatc aggtttgtta
     3661 atcagctgtt caaggagcga aggatcaagc aggcggctct tcatggcctt tatccgtgca
     3721 ttgacgtacg catactccat atcactcacc gaataggagg gagaagatct cgggtcgata
     3781 gatctccttc gcttttttaa gccgggattc aagagtgttg aatatcacaa agcgtccatc
     3841 atggctgtaa gcattcaacc ctccggcaga ggtcagatca gtaataattt cgccggagat
     3901 tttcatctcg ttcaggatat ccttgaggag cgcctcatct cttttatcga tgtgaaaaat
     3961 aacgccatcg ctgccgagat caccgattgc ttctttgagc agagaattca ggatctcttt
     4021 gtatctcgaa tgttcccgag aagattccag attccggacg gcttcatcaa agactctctc
     4081 aaaggtctca tttttggctt ttatgagctc catccggctc ttttcccgaa cttcggagag
     4141 gatcttgttt ctctgtatcg ctaccgattg ggttgcctct tccatgagcc gttttttgat
     4201 tggctggtcc tttgcctgtg catctttgat aatttcatct gcctcggcct ttgagcgttc
     4261 ctgaatctcc agaattcgtt cctgaccact cacttcaaca gccttgatca gatcgtcaat
     4321 tgccatagta cacctgttct ttccgacgtt ccagtgggat tccttcgcca gattatgccc
     4381 ggattatctc gcaggctcac accagcatga tcatgatcat tgatgcaact acaaaaccaa
     4441 gaataacgag agtctcggga attgccacca gaataatcat cagtccgaac atttccggtt
     4501 tttctgcaat cgttcccgct cccgctgcgc caattcttga ctgtgcaatc ccggtaccga
     4561 gcgctccaag cccgaatgcg atgcctgccc caatggggat tccgaaatca accattcaca
     4621 tcagcctcca tccaaagcgc cgcccctgca caggcggcac atcgtttttc ctcataacca
     4681 tactttgcct tcaaactcac tcaatgttga agagcgatga gaacacctct ctaaaaactt
     4741 caattacatt gacaactcct ctccttcttt ctcgattgat gataccgttt ccatgcggac
     4801 atctttatgc ttcaaaccga tttcccctcc tcctcctttt tgaaaggttt atagagcttc
     4861 ccaccaccct cgtaaaactt cgagtcaaat tcgacaaggt gcagacggag tgagtgaagg
     4921 aacggactga acatggcaag gacgagattg agcgtgtgaa gaagcaccgc gataataatc
     4981 ccgacaatgg caacctccat catgcctcca agtttgttag caaccacagc gaggatgact
     5041 gatgccatgc cgattgccat gatacgggag taggagagaa tgtttgtaac cgtactcatg
     5101 atctcaaacg ttccaaaagc accacccccg ataattatga gggggatggc gatgatgatg
     5161 aggaccgctc ccgttgtaat taaagccgtg ggcagcgtca ttgccacacc ggcaatcacc
     5221 aatatgagac cggttattac aataatcata cccgatttct cgcagatatg tttctttgcc
     5281 ttgtccacgt gtcttttaca acttaactcg gtataggagt tcactattcc cagaatcagc
     5341 ccgaggaata catggaagac acctattgca atcgaaagga tcaagaaggg gactatcgct
     5401 tccatccggt tccaggttac accaaagaat gtctttggct cgatccatcc catatgttcg
     5461 cctaaatcgc cgaaaaactc cccgtataac catccaaaga aaatcgacca gagtgcaccc
     5521 atgatcagga cgttcatcag gtgctgaagc caatcgcgat cggcgaatct ccacttcatt
     5581 atgatagcaa aagcaagaat tatcagcccg tatccgatgt ccccgaccat gattccaaag
     5641 aacagcggaa gaaaaatcgc cataatggga ctcgggtcaa attcagtgga tttggcaggg
     5701 ctgacaaccc gcatgaggaa ctcgaacggt ttgacaaaac tcgggttatc ataaaatgtc
     5761 ggggcctctg ccatctcatt cggggtaaga acaagttcat gaacaattac cctcccctca
     5821 aatgtatcct tgagggcatt cttcatctct ttgagggctt tctttggtac ccatcctgca
     5881 agtacaaagg tatattcggt ctggccgatc ttcgaaaaga tactcagttc ttctgttctc
     5941 tcaccaagga tccggtgtaa tgcgctgagt tcagtgtacg atgttgacga aattttttcc
     6001 agttcagcat tcagccggga aatttcctgg gtggaagagg tttttctctc ctcgataagg
     6061 ttaaaaatct cgttgaaagg ttttcccaga tattccgggg gtagccggac ctcattcaca
     6121 ttctggttga agaagtaaga gtggacctgt tccgaatacc ttttattgaa tatagtgacc
     6181 gcagcgatgg ttgtttcatc cagttcggca gacatcaatt caaactgccg ttttgtaatc
     6241 tcggcaaggg cattccggat gatatcaata acgtccttga actccttctg gatgagaaga
     6301 accgtaactt caaagccctc gagcttcggc atgctcgtct caaggggccg gattttatcg
     6361 ataattttct cgtaacgacc gaggttgatg agggcaagtt cgagatcgct cttctctttt
     6421 gcgagagttt ttgtggccat ttcgagctcg cttatgacgg tattagcccg agcgaccaga
     6481 ctttcatggc tcatcgcttt cagacttgaa taggaatatg ctacggcttt ctcatccgtc
     6541 ttgatcttgg gaagagtgag gagaatgccc ccgatctttg aatgcgcaac cgcgatatcg
     6601 ccaacctgtt ctgccgtcat ttttttcagg gtgatttccc cgggggtaac cggtacagac
     6661 gcatcctcga tatgcacagt ccccttggca tacagtacat ccagcacatt aggaaggtct
     6721 tttttgggac ccagtacctg tatctttacc attttctgga gcatggcact ctaccctgaa
     6781 aggattattg aggtgatcct ctcaactgct ctcggtaaat tattctcccc tctctgacgg
     6841 accgattcaa cctcgttcct ggccttggtc ctgatctgat ctgcttctgc ctgaaccaat
     6901 ttcatctcgc tctccaggaa atcatcagct gcctttttcc cctccttttc ggagttggta
     6961 attatctcca gggaatcttt cctggcccgg tcgatcatat gatcagctcc tacccgagca
     7021 tcgtcgatct ttacgcttac ctccagttct ttttcccgga ttttcgctaa tagtgacttt
     7081 tcaacaggca tgctttattc ctcgttacgg accttggatg tcaccataag ctctcctcct
     7141 caactaatgg tgattatcta cagaccatgt ctgcttttca ggagtgatga aactccgaac
     7201 tgtgggatcg ggctatcatt cctgaagcca gaccctttcc ctgcattggc tattcgctcc
     7261 tcgcagcgtc tcatagccga tattttcagt ctctcgatca tttcgctgtt tgaaagtgac
     7321 atacggattt cccgcaatcc ttaagcggga ttggtgaacc cttgatttca cagatgataa
     7381 attttcccct cgttctcttt tcgtgcgaga aatcagatct gattgtatcg ataatttttt
     7441 ttacggtaac atcccagcct gattctcaca ttatttaaat accatgccac tcatttcagg
     7501 tataggcgaa aggacgggtc aacgggatgt tccacaaaat attattcgct accgattttt
     7561 ctgaatatgc gaataagact ctcgactgta tcgcagggct tcccggggct cgtgaaatta
     7621 ttcttctcca tgtcctcgaa gaagcaagga atccaagagg cggaggggaa attgttgaaa
     7681 cacttacccc ggatgagaga aatcgcctca aagaagcgaa gcaccgactc gaatccctga
     7741 ataaaagtct caaggtggtt acccgggtga aaacgtcatc tgatattccc ggtgcaatca
     7801 tcgaagccgc cgaagagaac ggggtaaccc tgatcgttgt tggtgctcgt gggaacagta
     7861 tcgtggaagg tatcctcctt ggaagtgtat cggttgcgat tgtccggagg agtaagatca
     7921 cggtccttgt catgcgtcat aagattgtcg cgggtctggc gggaagggcg tatgaaatgt
     7981 ggtgcccgat gatcctctca aggattctct gcccgatcga cttctcggtc tgctccgatc
     8041 gtgcgataac gcttctcaaa acaacgaaag gggttggaca ggtcattctc ctccacgtgg
     8101 tctcccatgg ggagtctgaa tctgaactgg agaccactgt ttcggaagca ggggtaaagc
     8161 tcgccgagat tgggagagac ctctcccagg caggggtaaa cgtccggaca ctcgtaaaaa
     8221 gggggaatcc gggatcggag ataacaagaa ttgcggaaga agaggatgtc tcagtcatct
     8281 ggatcagttc acttggaaaa gggtggttca gggaacttct tctggggagt accgcatatg
     8341 atgtggccat gaactccaag cagccggtta tcatcgtccg tacaacgaaa tcaaagagag
     8401 aaaatcaggc actatcggag aacaaccaat accggtatga tgttgcctga ttgacagatg
     8461 gtgtttttca gatcttttat tgtgaaaatg gattcgtttc cggtaaaatg gggatagttc
     8521 cagatttacc cgcttgattc atactccggc aagcctgcca aggacacc
//
LOCUS       DAOOCO010000069         8394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_7997_length_8394_cov_5.158772, whole genome shotgun sequence.
ACCESSION   DAOOCO010000069 DAOOCO010000000
VERSION     DAOOCO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7997_length_8394_cov_5.158772"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..889)
                     /locus_tag="PK154_05265"
     CDS             complement(77..889)
                     /locus_tag="PK154_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW10505.1"
                     /translation="MSIAVVFDSAGTLLHTYRVAKDVEQQRLLPGIETVTLTFSSPER
                     VLVVIHVHSREIIGSDHEILLSRYLVSHQAGFGISCTRKITTAEDIGDVLYHDTHARI
                     GDLQDCIKNVWQVCKRESVVTLNSGVILNMAKKTIEFTVTTGGRPFEGAKEAIRDLHQ
                     RGIPTFIASGDRVAKLEKMADYLGIPRDRVYGVATPTVKAQIVADLQCEYDTVVMVGD
                     GINDLCAMKKADLAILTEEQPGDKPGELYQAAHHVVKNVREVVEIVEKLSDE"
     gene            949..1893
                     /locus_tag="PK154_05270"
     CDS             949..1893
                     /locus_tag="PK154_05270"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HPW10506.1"
                     /translation="MLVSDTAEKIKKMEIRGAGRIARAAVAALKAHSETAPSRDLSEY
                     RKEMVTAADLLIATRPTAVSLPNAVHLVMRSLTLDTPSVSSAKEEFSRACQDFIRSSE
                     NAIQRIGEVGARHIRDGDVILTHCNSEAALECLLTAHRKGVRFSVYATEVRPGNQGLL
                     TIGTLNRAGIPTTYIVDSAVRYFMKEIDRVIVGADAVSVNGAVVNKIGTSQIALAAHE
                     ARTSFLVAAETYKFAPRTILGEYIEIEERASSEVLPDPIAKANPFITVRNPVFDVTPP
                     EYVNLIVTEEGAIPPQMAYIIIRDYLGWEIGDFEARTR"
     gene            2011..2829
                     /gene="dapF"
                     /locus_tag="PK154_05275"
     CDS             2011..2829
                     /gene="dapF"
                     /locus_tag="PK154_05275"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPW10507.1"
                     /translation="MEITFVKMHGNGNDFILIDEYEKTVIPDEMKPQFAQLYCDRRFG
                     IGADGVLYLSRSGSADLKMRLFQPDESEAPMCGNGIRCFAKYAFDTGYVEKSCTVETM
                     AGIINVVMDYTDDTFLATIQMPEPRFDRADIPATGEGEYTETIEGYMVYAVNTGVPHA
                     VIFVEDPESFPLIEIGPAIRHHASFPEGANVNIVKREGEDHISIRTYERGVEDETFSC
                     GTGATAAAAIARHLGYTGTKVKVDTRGGPLVITLGEKTLMEGPAETVFKGAITF"
     gene            complement(2858..3556)
                     /locus_tag="PK154_05280"
     CDS             complement(2858..3556)
                     /locus_tag="PK154_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPW10508.1"
                     /translation="MTLADLTVAFTAGLLSPLGAACVLPLYPGYLSFLAAQQREPAVE
                     SRIPCIRILLGFIAGAGILAAMLGFGLLVGVIFTLPIGDVLSVVSPVAYAILGGIGIL
                     LVLGAGVRLPFPTPDILKGRSPLGGAFLFGLFFGILILPCNAGPVTVLLALSTSASDF
                     MSNLGNFLAYGMGMAIPLIAISAVSLYKGNRLVQALTTHRRSINIATGVLMIGVAAYY
                     LLVVFGIQDWMFSS"
     gene            complement(3553..4125)
                     /locus_tag="PK154_05285"
     CDS             complement(3553..4125)
                     /locus_tag="PK154_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10509.1"
                     /translation="MTDQYQRRKSQWRKRAGTITGMCLLFLFLCTAGCSTKETHQNGE
                     QIAWQTTLLTDVRTGTQFSIAEMNGTPLLIQPFTITCPICMQQQAAISRLYAQEEIPF
                     VMVGLDIDPNGNENSLHDYTRDHEYYGFYARSPPELTTALVDQFGLRVLSPAQAPLIL
                     VCPDGKARMLDPGLKSSEDLQRVLTEGCQA"
     gene            complement(4122..4805)
                     /gene="radB"
                     /locus_tag="PK154_05290"
     CDS             complement(4122..4805)
                     /gene="radB"
                     /locus_tag="PK154_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HPW10510.1"
                     /translation="MTTARLSTGVQALDDLLGGGLEPRVITQIYGGPASGKSVLCVLA
                     SVSCLKEGKSVIFIDTEGFSIERFRQVAGEDAERLADHLFLYEPHDFEQQGLVIKGLD
                     RPLSRGGIGLIVMDSSTGLYRTTLEKGHDAMQNLSRQMVHLLGYAKRYEIPVVITNQV
                     YMDVQKNTFVGLGGTALAHISKAIIRIDILNRHRKATLEKHRSIAPGRCFEFDIVETG
                     IVVRTVDAI"
     gene            complement(4802..5989)
                     /gene="larC"
                     /locus_tag="PK154_05295"
     CDS             complement(4802..5989)
                     /gene="larC"
                     /locus_tag="PK154_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPW10511.1"
                     /translation="MRLLVFDPIRGAAGDMVTGALLHVGADRDATVSAMASVVGEPQI
                     SLVERAGIRSIQVKTRATVERRTFPEVIRRVKNARASDSAIAMAVRVFERIERAEKEV
                     HGHTDHFHEVGADDAVAEVVGACTAFLSLHVDGCIVLPVMLGGGSITGSHGTYPAPAP
                     ATLSIFRQSGLRVMLGEPDDGELCTPTGAALLSEFSSVSALSRHACSVVCSGYGAGTK
                     NPPQIANVLRAMIVEYTGDNEPGDVDLLETNVDDVSPEIISYTVSRLIEAGAYDVSAI
                     PCTMKKGRPGHLVRVIAASGDGERLAAVMAEELGTLGIRSIQAVHRFIAERSVSEIPV
                     TIMGKERMIRVKCGKLHGNVISIKAEYDDVSAFAHELGIPARTIARLTENKAWDIIGM
                     FMK"
     gene            6036..>8394
                     /locus_tag="PK154_05300"
     CDS             6036..>8394
                     /locus_tag="PK154_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HPW10512.1"
                     /translation="MPEIFLKVDSAYPGDQGGGKARLDPETMLYLKISPGDIVAVEGK
                     RRTVAKVWRSLVEDWNQNKVRIDNFTRTNAGVSIGDSVKIVAIRDEIEARRVVLAPPE
                     DLPRSIPVSNNPNVMNALIDFPIVKNDTVPIMIGLPFLQPQIVSYKVVEIEPEQAVII
                     TKNTQIEFSEKPAAGFEGLKRISYEDIGGLKDELQRVREMIELPMRHPELFQKLGIEP
                     PKGVLLYGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQKLREIFEEAES
                     NAPSIIFIDELDSITPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRVDAI
                     DPALRRPGRFDREIEIGVPSEPDRTEILKIHTRGMPLAGEVDLLVLAQQTHGFVGADL
                     AALAREAAIRALRRYLPEMNLDDEEIPQEILDRMEVNAPDFRSALRDVSPSAMREVML
                     EVSHVTWDDVGGLVEAKQEIREAVEYPLTDRERFEELGISPPRGVLLYGPPGTGKTLI
                     AKAVANESGANFIAIRGPQLLSKWVGESERAVREVFKKARQVAPSIIFFDELDALAPT
                     RGSDLGSHVMETVLNQILTEIDGLEELRDVVVMGATNQPTLVDPALLRAGRFDRLVYI
                     GEPNLADRVKILGIHLKYLPLEGSKLEELVGKTGSCDEAALDTFIEILGKERTFTLDE
                     AIGALGTICSGGEGKMNRGARRRYMVDRMLQCGLSLIDEVRDELILNIAARTEGYVGA
                     DMEGLCREAGIFAMRERAAFVTLRHFEMSLAKVHPTMNERLREFYS"
BASE COUNT         2035 a         2166 c         2288 g         1905 t
ORIGIN      
        1 ttcattcctc tcctctcata acctaatgtt gtattcgtgg catattactc tttatatcaa
       61 tgcatccgtg cgggagtcat tcatctgaca gcttctcgac gatttcaacc acttcgcgga
      121 catttttcac cacatggtgg gctgcctgat acagttcacc gggtttgtca cccggctgtt
      181 cttcagtaag gatggcgaga tccgccttct tcatggcaca gagatcattg ataccgtctc
      241 cgaccattac tacggtatca tactcgcact ggaggtcagc cacaatctgg gccttgacgg
      301 tcggtgttgc aacgccatac accctgtccc gcggtattcc cagataatca gccatcttct
      361 ccagctttgc tacccggtct ccggatgcga tgaaggtggg aatacccctc tggtggagat
      421 ctcgtatcgc ctcttttgcc ccctcaaagg gtctcccccc tgttgtgaca gtgaactcga
      481 tggttttttt tgccatattc aggatgaccc cggaattgag ggtgacaacc gattcccgtt
      541 tgcagacctg ccagacattc tttatgcagt cctggagatc tccgatcctc gcgtgagtgt
      601 catggtaaag aacatctccg atatcctcag cggtcgtgat ctttctcgtg caactgatgc
      661 cgaatcctgc ctgatgggat accagatacc gtgaaagaag gatttcatga tccgatccga
      721 tgatctccct ggaatggaca tggataacga caagtacccg ttcaggagaa ctgaatgtga
      781 gggttactgt ctcaatgcct gggagtagcc gctgctgctc cacgtccttt gcgacacggt
      841 aggtatgaag cagtgtcccc gcgctatcaa acactacagc tatcgacatg gccggttgga
      901 atcccgcaaa gttctttgga ctctgctggc atgtactacg tacagtctat gctggttagt
      961 gataccgctg agaaaataaa gaagatggag atacgggggg ccgggaggat tgcacgtgca
     1021 gctgtcgccg cactcaaggc acactcggag acagcaccct cgagagatct ttcagagtac
     1081 cggaaggaga tggtaactgc agccgatctc ctcattgcta ccaggccgac tgcagtctcc
     1141 cttccgaatg ctgtccatct ggtgatgcgt tcattaaccc tggatactcc ctcggtttca
     1201 tcagcgaagg aagagttttc aagggcatgc caggatttca tccgttcgtc agagaatgcc
     1261 attcagcgta ttggcgaggt gggagcacgc catatcaggg acggtgatgt gatccttacc
     1321 cattgtaatt ctgaagcagc ccttgaatgc cttctcactg ctcataggaa aggtgtcaga
     1381 ttctctgtgt atgcaacaga ggtgcgaccg ggaaaccagg gtctcctgac tatcgggacc
     1441 ctcaaccggg ctgggatacc taccacttac attgttgatt ccgcggtccg ctatttcatg
     1501 aaagaaatcg acagggtgat tgttggagca gatgccgttt cggtaaatgg ggccgtcgtg
     1561 aacaagatcg gaacatcaca gattgcactg gctgcccacg aagcaaggac cagtttcctt
     1621 gttgctgcgg agacctataa attcgccccg cggacgatcc tcggtgaata cattgagatc
     1681 gaggagcggg ctagctcaga ggtgcttccg gatccgatag caaaagcaaa tcccttcata
     1741 acggtgagaa atcccgtttt tgatgttacc ccccccgagt acgtgaatct cattgtcacc
     1801 gaagaggggg caatacctcc gcaaatggcg tatattatca ttcgtgatta tctgggatgg
     1861 gagattggtg actttgaagc acgtacaagg tgatcgcgtc gatgcagatg caccagagac
     1921 gtacttgcca tcagaatcag ccggggactg ggaccttgga ggattatgat tagtacgcca
     1981 gataaaaaac agaccggagt gtaagtgaca atggaaatta cttttgtgaa aatgcatggc
     2041 aacgggaacg attttattct tatcgatgaa tacgaaaaga cggttattcc tgacgaaatg
     2101 aaaccccagt tcgcacaatt gtattgcgat cgaaggtttg gaatcggtgc ggacggcgtc
     2161 ctctacctct cccgttccgg ttcggccgat ctcaaaatgc gtcttttcca gccggatgag
     2221 agtgaagccc cgatgtgcgg gaacggaatt cggtgttttg caaaatatgc attcgatact
     2281 gggtacgtgg aaaagagctg tactgtcgag actatggcag gaattatcaa tgttgttatg
     2341 gattacaccg atgatacctt ccttgcgacc atccagatgc cggaaccacg gttcgaccgt
     2401 gccgatattc ctgcgaccgg tgagggtgag tacacggaaa caattgaggg atacatggtg
     2461 tatgctgtca ataccggtgt tccgcatgcg gtgattttcg ttgaagaccc tgaatcgttt
     2521 cccctcattg agatcgggcc agccatccgt caccatgcct catttccgga aggggcaaat
     2581 gtcaatatcg taaagcgtga gggagaggat catatcagca taaggacata cgagcgcggg
     2641 gtggaggatg agaccttctc ctgcgggacc ggagcaacgg cggcagctgc gatcgcccgg
     2701 catctcggct atacagggac gaaggtcaag gttgacacac gaggtggacc ccttgtcatc
     2761 acacttggcg agaagactct catggaaggc cctgccgaga cggtttttaa aggggctatt
     2821 accttctgat catattctgc tctctgaatg gcgggagtca ggaagagaac atccagtcct
     2881 ggattccgaa aacaacaaga aggtaatatg cagcaacacc aatcatcagg acccccgtgg
     2941 cgatgttgat cgatcggcgg tgggtggtga gagcctggac aagccggttc cctttgtata
     3001 aggaaacagc tgagatggcg atgagtggta ttgccattcc cataccatag gccaaaaaat
     3061 ttcctagatt ggacataaaa tccgaagcac ttgtgctgag cgcaagcagg accgtcaccg
     3121 gtcctgcatt acagggcagg ataaggattc caaaaaagag tccgaataaa aaagcccccc
     3181 ccagcgggga tctccctttc aggatatccg gtgtcgggaa tggaagacga acacccgctc
     3241 ccaggacgag gagtatccca ataccgccga ggatggcata cgcaacaggg gagacgaccg
     3301 acaatacatc accgattggg agggtgaaga tcactcctac aaggaggccg aatccaagca
     3361 ttgcagcaag gatccctgcc cctgcaataa atccgagcag tatccggatg cacggaattc
     3421 gggattcaac tgccggttct ctctgttgag ctgcaagaaa agagaggtac ccgggataga
     3481 gagggagaac gcatgcagca ccaagaggag agaggaggcc tgcagtgaat gctacggtaa
     3541 gatctgccag ggtcatgcct ggcagccttc agtaagcacc ctctggagat cctccgaact
     3601 cttcaggccg ggatcaagca tccttgcctt cccatcaggg catacaagaa tcaggggcgc
     3661 ctgggcaggt gagagcacac gcaacccgaa ctgatcaacc agcgctgtgg tcaattcagg
     3721 cggtgagcgg gcatagaaac catagtattc gtgatcccgg gtatagtcat ggagtgaatt
     3781 ttcgttcccg ttcggatcaa tatccagccc taccatgacg aagggtatct cttcctgggc
     3841 atagagtctg gagatcgcag cctgctgctg catgcagatc ggacaggtga tagtaaaggg
     3901 ctggatgagg agtggggtgc cgttcatttc agcgatgctg aactgcgttc ctgtccggac
     3961 atcggtaagc agtgtcgtct gccaggcaat ctgttccccg ttctggtggg tttcctttgt
     4021 tgaacacccc gctgtgcaaa ggaaaagaaa aagaaggcac atccccgtaa tagtacctgc
     4081 ccttttcctc cattgactct tcctcctttg atactgatct gtcatatggc atccaccgtc
     4141 cttaccacta ttcctgtctc aacgatatca aactcaaaac atcttcccgg cgcaatggac
     4201 cggtgtttct caagggtcgc cttcctatgt ctgttcagga tgtcgatcct gatgatagcc
     4261 ttcgagatat gggcgagtgc ggtgccaccc aggccaacga acgtgttctt ctggacatcc
     4321 atgtacacct ggtttgtgat caccacggga atctcatatc tcttcgcata cccgagcaga
     4381 tggaccatct gcctggagag attctgcatt gcatcatggc ctttttcaag cgtcgtcctg
     4441 taaagaccgg ttgatgagtc catcacgata agcccaatcc ctccccttga gagtggcctg
     4501 tcaagacctt tgatcacgag gccctgctgt tcaaaatcat gcggttcata aagaaaaaga
     4561 tggtcggcga gccgttccgc gtcctcccct gctacctgcc ggaaccgctc aatagaaaat
     4621 ccctcggtat cgataaaaat cacgcttttc ccctctttca ggcaggagac agaggcgagc
     4681 acgcagagta cgctcttacc acttgccgga ccaccgtata tctgggtgat cacccggggc
     4741 tcgagtcctc ccccgagcag gtcatccagt gcctgcacac cggtgctcag tctcgcggtc
     4801 gtcatttcat aaacatcccg ataatgtccc atgccttgtt ttcagtgagc cgggcaatgg
     4861 tccgggcagg tatccccagt tcatgagcga atgcgctgac atcatcatac tctgccttga
     4921 tcgagatcac gttaccatgc aacttgccgc atttaacccg tatcatccgc tcttttccca
     4981 tgatggtgac agggatctcg ctcaccgaac gctctgcgat aaaccggtga actgcctgaa
     5041 tcgagcggat accgagagtt ccgagttctt cagccattac tgctgccaat cgttctccat
     5101 cgccagaagc tgcaatcacc cggaccaggt gtccaggtcg tccttttttc atcgtgcagg
     5161 gtattgcgct gacatcgtat gctcctgctt ctatcagccg tgacaccgta tacgaaatga
     5221 tttcagggct tacgtcatcg acatttgttt caagaaggtc aacatcaccg ggctcgttgt
     5281 ctccggtata ttcgacaatc atcgcacgga gcacgtttgc aatctgtggg ggatttttcg
     5341 ttcctgcacc atatccggaa cagacaacag aacaggcatg cctcgagagt gctgacaccg
     5401 aggaaaactc ggagaggagg gctgccccgg ttggggtgca gagttccccg tcatcaggct
     5461 cccccaacat gacccggaga cctgattgcc tgaaaatcga gagtgtagcc ggtgcaggag
     5521 caggataggt gccatgggag ccggttatag atccaccgcc cagcatcaca gggagaacga
     5581 tgcagccgtc cacatgaagg gaaaggaatg ccgtgcaggc tcccaccact tctgcaactg
     5641 catcatctgc tcccacctcg tgaaaatgat cggtatgccc atggacctcc ttttcagccc
     5701 gttcaatccg ttcaaacact ctgactgcca tggcgattgc agaatcagac gccctggcat
     5761 tcttcaccct ccgaatcact tcaggaaacg ttctcctctc cacggttgcc cgggtcttaa
     5821 cctggatgct cctgatcccg gcacgttcaa ccagggatat ctggggctca ccgacaacag
     5881 aagccatcgc agagacagtg gcatcccggt cagccccaac atgaagaaga gcaccagtga
     5941 ccatatcccc tgctgcgcca cggatgggat caaagacaag cagtcgcata aattaccagt
     6001 acttataaat ccctgtgtat atgactttta aggtgatgcc tgagatattt ctgaaggttg
     6061 atagtgctta tcctggtgat cagggtggcg ggaaggcacg gcttgatccc gaaacaatgc
     6121 tgtacctgaa gatatctccc ggcgacattg ttgcggtgga aggaaaacgt cggacggtgg
     6181 ctaaggtttg gcgttccctt gttgaggact ggaaccagaa taaggtcagg atcgacaatt
     6241 tcacgaggac caatgcaggt gtcagcatcg gagacagcgt gaaaatcgtc gcgatacgtg
     6301 atgaaattga agcacggagg gtggttcttg caccgcccga agatctcccg cggagcatcc
     6361 cggtaagcaa taatccgaat gtgatgaatg cattgatcga ttttccgatc gtaaaaaatg
     6421 atacggttcc gataatgatt ggacttccgt tcctccagcc ccagatcgtc tcctataaag
     6481 ttgttgagat tgagcctgaa caagcagtga tcataacaaa aaatacccag atcgagtttt
     6541 ccgaaaagcc ggcagctggc tttgaagggc tgaaacgaat atcatatgag gatatcgggg
     6601 gattgaagga tgagctccag cgtgtgaggg agatgatcga actgccgatg cgtcatccgg
     6661 aactcttcca gaaactcggg attgagcccc cgaaaggggt tctcctgtac ggacctccgg
     6721 ggaccggaaa gaccctgatt gcaaaggcag tggcaagcga gagcggtgcc catttcatct
     6781 caatcgctgg tcccgaagtc atctccaagt attacgggga gagcgagcag aaactgagag
     6841 agatctttga agaggcggaa tccaatgcgc cctcgatcat cttcatcgat gagcttgatt
     6901 ctatcacacc cagaagggaa gaagtgaccg gtgaggtgga acggagggta gtggcacagc
     6961 tccttaccat gatggatggc ctcgaggaga ggggacaggt ggtggtcatt ggagctacca
     7021 accgggtgga tgccatcgat cccgctctca ggagaccggg ccggtttgac cgggagatag
     7081 agattggtgt cccgagtgag ccggacagga ccgagatcct gaaaattcat acgaggggga
     7141 tgcccctggc cggcgaggtg gacctgctcg tgcttgccca gcagacccac gggtttgtcg
     7201 gcgctgatct tgccgcactg gcaagggagg cggctatccg tgcattgaga cgctacctcc
     7261 ccgaaatgaa ccttgatgat gaggagatcc cccaggagat cctcgacagg atggaggtaa
     7321 atgcaccgga cttccgatca gcactccgcg atgtaagccc gagtgcaatg cgcgaggtga
     7381 tgctcgaagt cagccacgtt acctgggatg atgtcggcgg gcttgtggaa gcaaaacagg
     7441 aaataaggga agcagttgag tatccgctca ccgaccggga acggtttgag gagctgggga
     7501 tctccccacc ccgcggggtg ctcctgtatg gacctcccgg aaccggaaag accctgatcg
     7561 caaaagcggt ggcaaatgag agcggcgcaa attttattgc aatacggggt ccgcagctcc
     7621 tgtcaaagtg ggttggtgag agcgaacggg cggtccggga ggtgttcaag aaagcccggc
     7681 aggttgcccc ctcaataatt ttctttgatg aactcgatgc cctggcacca acccggggat
     7741 ccgatctcgg ttcccatgtg atggagaccg tgctgaacca gatccttacc gagattgacg
     7801 ggcttgagga actcagggat gttgtggtga tgggagcgac gaaccagccg accctcgttg
     7861 atcctgccct cctccgtgca ggccggtttg accgtcttgt gtatatcggt gagccgaacc
     7921 ttgcagacag ggtaaagatc ctcggaatcc atctgaaata tctgcccctc gagggatcaa
     7981 aactcgagga gctcgtagga aagacgggat cctgcgatga agcagcgctt gataccttca
     8041 tcgagatcct tggaaaggaa aggacgttca cccttgatga agcgattggt gcgctgggca
     8101 caatctgttc cggcggggaa gggaagatga accgaggggc tcggagaaga tatatggttg
     8161 accggatgct ccagtgtgga ctttccctca tcgatgaggt gcgggatgag ctgatcctga
     8221 atattgccgc aaggacggaa ggatacgtcg gcgctgatat ggaaggactc tgccgcgagg
     8281 ccgggatttt tgccatgaga gaaagggctg cattcgtaac gctccgccat tttgagatga
     8341 gtcttgccaa ggtgcatccc acgatgaacg aacgtctcag ggaattttat tccc
//
LOCUS       DAOOCO010000070         8299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8111_length_8299_cov_5.470160, whole genome shotgun sequence.
ACCESSION   DAOOCO010000070 DAOOCO010000000
VERSION     DAOOCO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8111_length_8299_cov_5.470160"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PK154_05305"
     CDS             complement(<1..587)
                     /locus_tag="PK154_05305"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPW10513.1"
                     /translation="MITPGYEIQLMMVAGWLILIVLLQLALWPLLRPVLKEFSFPASF
                     PASLLLFTLVSWYCGLLHLPVWIAVLPFVALLFWFAYKGCYRRDTFRGQGVWAFVFFL
                     FFLFMLELRFINPGISFAEKFMDHAFLASIMRVPVIPPLDPWFLGGTLNVYYYLGYWM
                     FGTLGIVSGVPSPIVFNLALPTVLANAAVGMYAMA"
     gene            complement(568..1539)
                     /locus_tag="PK154_05310"
     CDS             complement(568..1539)
                     /locus_tag="PK154_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPW10514.1"
                     /translation="MLRKISAIAVSTLLAVGIILFMLYRVKDDLLVALEHIVWFYCAL
                     AVAVCSLAWLLRGVRYRIILSGIGIRCSILFSTGCIFISQTANLIVPARLGDLVRVFI
                     LRHEFGSTISQGISSLVVERVFDIVMVALLGAVSLLFVFNVPGEYIWLIVLPLILGAG
                     FFVFLSISDRFSSDNRYLRILLTMLDQIRQVSLSAKSILALSSSSVVIWILDVLVCLF
                     VGMMFGVTIPFAVVTLAIVVGNLVKAIPLTPGGIGTYELALVAIFSAAGIDPAVATLI
                     AVIDHLLKNLVTLGGGVISIYFFGDWVIDAIKSAFKKELPEDDHPGV"
     gene            complement(1523..2239)
                     /locus_tag="PK154_05315"
     CDS             complement(1523..2239)
                     /locus_tag="PK154_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW10515.1"
                     /translation="MTGVEVSAVIPVFNDREALKRAIPLSITRLGEVAPSFELIIAED
                     GSTDGSAELVRSWEEQDSRVRLFHSDERLGRGRALNRAFSAAEGAIVCYYDVDLATDM
                     KHLGTLIGAIRNGYDISTGSRLLPQSRIQRSGGREIASRAYNLLVRTVLGSRLHDHQC
                     GFKAFSRERILSIIPAVRDTHWFWDTEVLVRAGRRGLSICEFPVTWQQGGKTTVKRHD
                     VWDMGTAILRLWWDLHVEKD"
     gene            complement(2236..3459)
                     /locus_tag="PK154_05320"
     CDS             complement(2236..3459)
                     /locus_tag="PK154_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW10516.1"
                     /translation="MKVCIIGGGLAGLSAALRLKERHEVDIFEKKGHLGGCLSSHHID
                     TYWIEQFYHHCFSGDRHLLDLFSQLHIRDRLEWRKGTTGYYANNTIYPLNTPLEILRY
                     PLLSPTDKLRLALLTMRSKRTDPASLDDITAPEYIISEVGKRTYESFFQPLLTSKFGP
                     MSEKVSAAWLIGRIAIRSDRGLSGERLGYLKGGFQVFLDALADEVGGDCTIRLHDPVI
                     HMAKNTEGWRVNGQRYDAVLSTVPPQVLTSIGGPMLPPVPYQGAACLTLGMEEEYTQG
                     IYWLNMKDPGSFGAVISHTNFIPVERYGEHIVYLASYFTGTLPAQAGHLMREEFCNTF
                     SIPSSQVHWERFTVEPLAGPIFTTGYRNLIPHYQEKGLYFAGMFSASNYPERSMEGSV
                     VAGYRAAEAISEGGT"
     gene            3644..4822
                     /locus_tag="PK154_05325"
     CDS             3644..4822
                     /locus_tag="PK154_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HPW10517.1"
                     /translation="MRIFFIGFGQAGGKIVDMFLEQDRKLGRNSFRAIAVNTARTDLM
                     GLKHIELKDRILIGQTVVKGHGVGTDNAAGARITMDEVDSIVSAIDRKGTHDVDAFMV
                     VAGLGGGTGSGGTPVLVRQLKNIYHEPVYVLGILPAPEEGKLYSFNAARSLTTLIKEA
                     DNTFVFDNSAWKNEGESIKSAFSRLNDEIVRRFGVLFRAGEMGKAGVGEMVVDSSEII
                     NTLRGGGISTVGYAIASVTTDRSKSPLSGIIGGITSTLKKREATEEVLLGEDRTAKII
                     SLVRRAMLGRLTLPCDFSTAERALVLVAGPPDELDRKGIEKAKSWVEENIAGVEVRGG
                     DYPVRSSFVAAVVLLSTIGNAPRIRELTEIARETKEDSVRAEERKPLTIDEDIEPLFE
                     "
     gene            4943..5965
                     /locus_tag="PK154_05330"
     CDS             4943..5965
                     /locus_tag="PK154_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10518.1"
                     /translation="MSGQTEASASFTVNFVSSGGYTFDKENTLKMDTELADPTWTYII
                     IQDGVEFPAKTDAGPNIQLSGWELSYPSDREIALKVKVAGTAPVVTNSSEKTIMRVRE
                     LSSSNTLVGTEIVKKKMVLNPAAIGETIQSESARMSTLRQKLDQLAGSGIDLTTAEAK
                     YGQAAAYIQSAQKSTDIVRKSSDLSAAKKLLDEVDSSVAVMEAQHAISTAEGSIGQTE
                     QLITYFKVNKSMGSDARLMPIIARWEIAADKLSDAKDLQSGGKYAEAADKAAEAAAKG
                     DEVLNDALALQEKVDSNPLSGMLSGASGAISSVLIPILVVIVIAVIVIVAIVLFRKRR
                     KWDELG"
     gene            complement(6719..>8299)
                     /locus_tag="PK154_05335"
     CDS             complement(6719..>8299)
                     /locus_tag="PK154_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HPW10519.1"
                     /translation="EGIYAYEPMYHGPFLYYVTAGMFTLFGDSDLVARILPALFGVAI
                     IPLIYWIYRMGYLDQRQTLIAGLFVALSPCLVYFSRFLRHDIFQLFFTVLILAALLAY
                     TERGQLRYALLAGLAIGGGMTLKEDMPIFLLILATFAIYLLLTKKIRLPQTWLRDSIA
                     LVVVALAVMVIFYSSFGAHFEIIETGWIDAYKHWASMHGMCRICGPWFFYILLFLLYE
                     VPIILLAVYGSAQFAIRHNPLPRIAFSLKHLFSGKNGLTGAEENQGNIPQPPVPWNKK
                     EIFFLFCIYWMVVTLAAYAYIGEKVPWLIIHQLFPMILVSVYLMTEKKMYIALAGCIF
                     LVLMTWHVALVPADINEPIVQVQNSEDMRTVMKLIDVSDTVVIASENYWPLPWYYRGD
                     KWEKMRFYGKLVDAGTISSVNPDLVITHDMSSYPALEGYDKATYKLSYWFSIYDNEHR
                     IPEYYLKRDGKLGSINIDVFTKPGLYEKANVSFPTMQSTALTTEEPASQSRQRWDQKL
                     ANITVRMFGPDSYQKSAG"
BASE COUNT         2058 a         2112 c         2414 g         1715 t
ORIGIN      
        1 tgtgccatgg catacatacc aaccgctgca ttggcaagca ccgtaggcag ggcgagattg
       61 aagacaatag gggacgggac gcccgagacg atcccgagcg tgccaaacat ccagtacccg
      121 aggtagtagt agacattgag ggtccccccg agaaaccacg ggtcgagagg gggaatgacc
      181 ggcacccgca tgatcgatgc cagaaatgca tggtccatga acttctctgc aaaagagatg
      241 cccggattga taaaccgcag ctcgagcatg aataagaaga agaggaaaaa cacaaacgcc
      301 cataccccct gtcccctgaa ggtgtcacga cggtaacatc ctttgtaggc gaaccagaag
      361 agcagggcta caaacgggag aaccgcgatc cataccggga gatgcaggag gccgcagtac
      421 caggagacaa gggtaaaaag gagcagcgag gcaggaaatg acgccgggaa tgagaattct
      481 ttcaggaccg gccggagaag aggccacagt gccagctgga gcaggacgat caggatcagc
      541 catcctgcca ccatcatgag ctggatttca taccccgggg tgatcatcct ctgggagctc
      601 ctttttaaag gcggatttta ttgcgtcgat cacccagtcg ccgaagaagt agatcgatat
      661 caccccgccc ccgagggtga ccaggttctt gagcaggtga tcgatcacgg caatcagggt
      721 ggcaacggcc ggatcgatcc cggcagcgga aaagatggca acaagcgcca gctcataggt
      781 cccgatgcca ccgggagtca ggggaatggc cttcaccagg ttgccgacaa cgatggcaag
      841 cgtaaccacc gcaaacggga tggtcactcc gaacatcatc cccacaaaga ggcagacgag
      901 gacatccagg atccagatca cgaccgatga ggaggagagg gccaggatgg attttgccga
      961 gagcgatacc tgcctgatct ggtcgagcat cgtgagcagg atccggaggt agcggttgtc
     1021 ggatgagaag cgatcactga tcgagaggaa gacaaaaaaa ccggccccaa gaatgagggg
     1081 aaggacgatg agccatatgt attctcccgg gacattgaac acgaagagga gggagaccgc
     1141 acccaggagg gcaaccataa cgatgtcgaa cacccgttcc accacaaggg aggagatccc
     1201 ctgcgaaatg gttgatccga attcatgcct gaggatgaat acccgcacca ggtccccgag
     1261 ccttgcgggg acgatcaggt ttgcggtctg tgagatgaag atacagccgg tggagaagag
     1321 gatgctgcat cgaatcccga tgccggaaag gataattctg taccggactc cacggagaag
     1381 ccatgcaagg gaacagacgg ccactgccag ggcacaatag aaccagacaa tgtgttcgag
     1441 cgcgacaaga aggtcatctt tcacccggta gagcatgaag aggataattc ccaccgcgag
     1501 cagggtggat acggcgatgg cgctaatctt tctcaacatg caggtcccac cacagtctga
     1561 ggattgcggt tcccatgtcc catacatcgt gccgtttcac ggttgtcttt cctccctgct
     1621 gccaggttac cggaaattca cagatcgata agcctcttct ccctgccctg acaaggacct
     1681 cggtatccca gaaccagtgc gtgtctctga cagcagggat gatcgagagt atcctctccc
     1741 gtgaaaaggc tttgaaaccg cactggtgat catggagcct gctgccaagc acggtcctga
     1801 cgaggaggtt atatgccctg ctggcaatct cacgccctcc gctcctctga atcctgcttt
     1861 ggggaaggag acgcgaaccg gtggagatgt catacccgtt cctgatggca ccgatcaggg
     1921 tcccgagatg cttcatatcc gttgccagat ccacatcgta atagcagaca atcgctcctt
     1981 ctgctgcgga aaatgccctg ttcagtgccc gtcccctgcc aagccgctca tcgctgtgga
     2041 agaggcggac cctcgaatcc tgctcctccc atgatcggac gagctcggca ctcccatcgg
     2101 tgctcccatc ctctgcgatg atcagctcga aggatggcgc cacctcaccg aggcgggtaa
     2161 tcgagagggg gatcgctcgt ttgagtgcct cccggtcatt gaagacggga ataactgcac
     2221 tcacctccac cccggtcacg tgcctccctc tgagatcgct tcggcggcac gatatcctgc
     2281 gacgaccgat ccctccatgc tccgctccgg gtagttggat gccgagaaca tccccgcaaa
     2341 gtagagcccc ttctcctggt agtgcgggat gaggttccgg tatcccgtgg tgaagatagg
     2401 gcctgccagg ggttcaaccg tgaaccgctc ccagtggacc tgggaggagg ggatggagaa
     2461 cgtgttgcag aactcttccc gcatcagatg tcctgcctgg gccgggaggg ttcctgtgaa
     2521 ataggaggcc aggtacacga tatgctctcc gtaccgctca acagggatga aattggtatg
     2581 gctgatcacg gcaccgaatg aacctggatc tttcatgttc agccagtaga tcccctgggt
     2641 atactcctcc tccatcccca gcgtgaggca tgctgccccc tggtagggca ccggagggag
     2701 catcggcccg ccgatggaag tgagcacctg gggtggaacc gttgagagta ccgcgtcata
     2761 ccgctggccg ttcacccgcc atccttccgt gttctttgcc atgtggatga ccggatcatg
     2821 gagcctgatt gtacagtcac ctcctacctc atcggcaagg gcatccagga agacctgaaa
     2881 tcctcccttc agatatccca ggcgttctcc tgaaagacca cggtcggagc ggatggcaat
     2941 ccttccgatc agccatgcag cggagacctt ctcgctcatg gggccgaact tgctggtaag
     3001 gagcggctgg aaaaacgact catacgtccg ctttcctact tcggagatga tatactcagg
     3061 tgcggtgata tcgtcaagtg aggcaggatc ggtccgtttc gatcgcatcg tcagcagtgc
     3121 aagcctcagt ttgtcagtgg gagagaggag cggataccgg agtatctcaa ggggagtatt
     3181 cagcgggtaa atggtgttgt tggcataata gccggtagta cctttccgcc actccagcct
     3241 gtcgcggatg tgcagttgtg aaaagagatc aagcaggtgc cgatcgccgg aaaagcagtg
     3301 gtggtagaat tgttctatcc agtaggtatc gatatggtgg gaggagaggc atccgccgag
     3361 gtggcctttc ttttcgaaga tatcaacctc gtgccgttct ttgaggcgca atgcagcaga
     3421 tagtccggca agccctccac caatgataca taccttcatg gtcggattct gcagtatagg
     3481 tttgaaaagc gggataaaaa aggctccgca tacgtatacg ccgcatataa agccctttgg
     3541 aggcggtttg gtataagaaa gtttttgaac ttctgaaagt aactttataa atagaacatt
     3601 tcggataatt ccggaagaat cggccggctg gtgttttggg tccatgagga tatttttcat
     3661 tggttttgga caggcgggag gcaaaattgt cgacatgttc cttgaacagg acaggaagct
     3721 tggccgtaac agcttccggg caattgccgt caataccgcc cggactgacc tgatgggact
     3781 gaaacatatc gaattaaaag acaggattct catcgggcag acggttgtca agggacatgg
     3841 ggtcggcacg gacaatgcgg caggtgcccg tatcaccatg gatgaggtcg acagcattgt
     3901 cagcgccatc gatcggaagg gaacccatga tgttgatgcc ttcatggtcg tcgccggtct
     3961 gggtggaggg acaggatcag gggggacccc cgtccttgtc cgccagctca agaacatcta
     4021 tcatgaaccg gtatatgtgc tcggcatcct ccctgccccg gaagaaggga aactctactc
     4081 attcaatgca gcacggagtc tgacgacgct catcaaggaa gccgataaca cgttcgtttt
     4141 tgataacagt gcatggaaaa acgagggaga gagcatcaag agtgcattct cccggcttaa
     4201 tgatgagatc gtaaggagat ttggcgtact gttcagggca ggggagatgg gaaaagccgg
     4261 tgtcggtgag atggtggtcg attcgagcga gatcatcaac acgctccgcg gaggcggcat
     4321 cagcacggtc ggttatgcta tcgccagtgt caccacggac cggtcaaagt cccctctctc
     4381 cgggatcatc ggtgggatca caagcaccct gaagaaacgt gaagcgaccg aggaggtgct
     4441 ccttggggag gaccggactg caaagatcat ctccctggtg aggagggcca tgcttggccg
     4501 tctcacgttg ccctgcgatt tctcgaccgc tgaacgtgcc ctcgtccttg ttgcaggacc
     4561 acctgacgaa ctcgatcgga aggggatcga gaaagcgaag agctgggtgg aagagaacat
     4621 tgccggagtc gaagtgagag gcggggatta cccggtaagg agcagttttg ttgccgccgt
     4681 ggttctcctc tcaacgattg gaaatgcccc ccgcatccgg gagctgaccg agatcgccag
     4741 ggagacaaaa gaggattcgg tgcgtgcaga ggaacggaag cctctgacta ttgacgagga
     4801 tattgaacca ttgtttgagt gatgagagat gagcaggcag ttacagattt tgggaattct
     4861 gatagttttc atcagtttcg tcggtattgc ttcggcggcc gtttcggtag gaaatattgc
     4921 catactacca agtggagatc tggtgtcagg ccagaccgag gcatcagcat cgttcaccgt
     4981 gaattttgtg agttcagggg gatatacctt tgataaagag aataccctga agatggatac
     5041 cgagctggca gatccgacat ggacctatat catcatccag gatggagttg aatttcctgc
     5101 aaagaccgat gccggcccga atatccagtt gagcggctgg gagctctcgt atccatcgga
     5161 tcgtgagatc gcgctgaagg tcaaggtggc aggaactgcc cctgttgtca cgaattcatc
     5221 cgaaaagacc atcatgaggg tgcgtgagct gagcagcagc aacacgctcg ttggcaccga
     5281 aatcgtgaag aagaagatgg tcttgaaccc ggctgcgatc ggcgaaacca tacaatccga
     5341 atcagcacgg atgagcaccc tgcgccagaa gctggatcag ctggctggca gcgggatcga
     5401 cctgaccacg gcagaggcga aatacggtca ggctgctgca tatatccaga gtgcccagaa
     5461 atctaccgac atcgtccgca agtcaagcga tctctcggca gccaagaagc tgctcgatga
     5521 ggtggactca tcagtggcgg tgatggaagc ccagcatgcc atctcgactg ctgagggatc
     5581 cattggacag accgagcagt tgatcacgta cttcaaggtc aacaagagca tgggttccga
     5641 cgcccggctc atgccgatca ttgcgcgatg ggagattgct gcggataaac tctcggatgc
     5701 caaagatctc caatcgggag gcaagtatgc ggaagccgcg gacaaagctg ccgaagcggc
     5761 agccaaagga gatgaagtgc tgaacgatgc cctcgctctt caagagaagg tggactccaa
     5821 tcccttgtca gggatgttat cgggggcatc tggtgcgatc tccagcgtgc ttatcccgat
     5881 tcttgtcgtc atcgtgattg cagtcattgt gattgttgcc atcgtactct tccggaaaag
     5941 acgaaagtgg gatgaactgg gataatccct gcttcttatc ctctcgtttt cgctcctctt
     6001 ttttccctgg atcgaccggg gcattcctct ttccccccag cgtatcgcaa cacgggagaa
     6061 gatgcagaat tatccgggga caggaaacga gggaggtcat cctctcccct cccaggagag
     6121 cgaaaagact gattctgatg gctcgcgctg ggtgattttc cgggaatctc ttggttctac
     6181 cgtccccctt ccggagaaca tcagatcttt aaactctgaa ttctgtatga caatactgga
     6241 gagaccaggt cccatcctga tacagtggtg cttcttaagg ggagaaaagg aaagggtatc
     6301 gtactatttt aaccaccaat cctgcgatta aattacgttt aaaaatattc catttttgca
     6361 gttccaaaaa tcgatgaaaa aagttacatt ccatatagca attcacaatg aaggaataat
     6421 gcggttgata ccatcctatc gcatcttggg ggacgccaca gcggcggggg taagccaagg
     6481 gcgcaacccc caggagcgtc aagagaggta tgaaatcatt tgatggcgtg aaaaaatggt
     6541 ggatgaaaag ccactatacc caaggaaaaa tcaatcatca tattggtggt caggtctcgt
     6601 aaggcgggtg taaccgatac catcccgata ggttggcgca ttatggggga agaaaaggaa
     6661 aagtcagatt ttcatataag ggaggaggat tgtttcggta gtgggatgac ggaatccttt
     6721 accctgcact cttctgatag ctgtccgggc caaacatccg gacagtaata tttgcgagtt
     6781 tctggtccca gcgctgcctg gattgggatg ccggctcttc tgtggtcagt gcagtgcttt
     6841 gcatcgttgg aaaagagaca ttggcctttt catacagccc gggttttgtg aagacgtcga
     6901 tgttgatgct cccgagcttt ccatccctct tcaggtaata ttcagggata cggtgctcgt
     6961 tgtcgtagat ggagaaccag taggagagct tgtaggtcgc cttgtcatac ccctcaaggg
     7021 ctgggtaact gctcatgtca tgggtgatca ccaggtcagg attgacgctg gagatggtgc
     7081 cggcatccac gagttttcca tagaaccgca tcttctccca cttatccccc cggtaatacc
     7141 agggaagcgg ccagtagttc tccgatgcga tcaccacggt atccgacaca tcgatcagtt
     7201 tcatgacggt ccgcatgtcc tcggaattct ggacctggac gatcggctcg ttgatatcgg
     7261 cagggaccag ggcaacgtgc caggtcatga ggaccaggaa gatacaaccg gcaagggcga
     7321 tgtacatctt cttctccgtc atcaggtaga cagagacgag gatcatcggg aagagctggt
     7381 ggatgatcag ccaggggact ttctccccga tataggcgta ggcagcaagg gtcacgacca
     7441 tccagtagat acaaaagagg aagaagatct ctttcttatt ccagggaacc ggcggctggg
     7501 gaatgttccc ctggttctct tcggcccccg tcagcccgtt tttccctgaa aaaaggtgct
     7561 tcagagagaa agctatcctg ggcagtgggt tgtggcggat ggcaaactgg gcgctgccgt
     7621 ataccgcgag aaggatgatc gggacctcgt agaggaggaa gagcaggata tagaagaacc
     7681 atggcccgca gatccggcac atcccatgca tcgaggccca gtgcttgtac gcatcgatcc
     7741 accccgtctc gataatctcg aaatgtgccc cgaaggagga gtagaagatg accatcacgg
     7801 ccagggcaac cacgaccagc gctatcgagt cacggagcca ggtctgaggg agcctgatct
     7861 tttttgtcag caggagatag attgcgaagg tggccaggat aaggaggaag ataggcatat
     7921 cttccttgag tgtcatcccc cctccgatgg caaggcctgc gagcagggca taccggagct
     7981 ggcctcgctc agtgtaggcg aggagcgcag ccagtatcag caccgtgaag aagagctgga
     8041 atatgtcgtg gcggaggaac ctcgaaaaat ataccaagca gggagagagg gcgacaaaga
     8101 ggccggcgat cagggtctgg cgctggtcaa ggtatcccat ccggtagatc cagtagatga
     8161 gcggaatgat cgcgacgcca aagagggccg gaaggatccg ggcgacgaga tcgctgtcac
     8221 caaagagcgt gaacattccc gcagtgacat aatacaggaa cggtccgtga tacatcggtt
     8281 catacgcata aatcccctc
//
LOCUS       DAOOCO010000071         8275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8141_length_8275_cov_7.417640, whole genome shotgun sequence.
ACCESSION   DAOOCO010000071 DAOOCO010000000
VERSION     DAOOCO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_8275_cov_7.417640"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..261
                     /locus_tag="PK154_05340"
     CDS             91..261
                     /locus_tag="PK154_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10520.1"
                     /translation="MAQHSTSPVPLYLLPQALSEEIKKYGDTIAEIRIRRTTGHNYFL
                     KVKHERRGDRGD"
     gene            complement(375..1340)
                     /locus_tag="PK154_05345"
     CDS             complement(375..1340)
                     /locus_tag="PK154_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10521.1"
                     /translation="MARISRDRVVFSEKGIMLMNISTGSLAQLTNQDDAFLPVSDLRF
                     SQNPSIDGDRVVWTEHEGVSHSSVDGGKIVIFNLTTGENQYLPTGSPGHQSTPMISGN
                     FILWKDYRSHGPDDPELYLYDLMNGTEERLLTENPVKSTPYISGDDVVWTEEIHGIHT
                     IIHYSIASRVRTVVGQGSVHQGHLPPISDERVAWLQSMNPFDFRDSRSAIMVMDLRTG
                     EKTQITPFRRGLSHPVISGEYIIYTRGAGDDWSREPREVVLHTLSSRPGETGMSDTGR
                     SNSHSPVNSSLNQGGAIPPTSSPGFAYITLIGGCITALALRKVLR"
     gene            complement(1667..2302)
                     /locus_tag="PK154_05350"
     CDS             complement(1667..2302)
                     /locus_tag="PK154_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10522.1"
                     /translation="MKLYGIKMRSVFMLVLILVSALIGLANAEDEKGVVRSDATAEQL
                     SLINELYDKDITVLEYMEKVHPEHLVGIPDSYRKNMAGQKMTWWATEPGQDNDTNDDQ
                     PALDRATVSVSATGYKFDWRTIRFGGTCTASGLEEPPWYIYVEAFLINRGTGQTVDST
                     SASSQQGVWSVTASKDKLYPANGQYYVHAWGYIPTPYYAEDYDDTSVFSFP"
     gene            complement(2723..3256)
                     /locus_tag="PK154_05355"
     CDS             complement(2723..3256)
                     /locus_tag="PK154_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10523.1"
                     /translation="MEKTTLIFLFFATFILVIGVLSLITSQVTDISSMDRRNEKWEPP
                     NVTPGKQHLGIPVWFHPSRIEIHAGEIKEEQIILKTRAKGQGLVIQRIVPVKEKSVNS
                     DEEIPMPRYLNVSMSPSEYVVSPNGTYVSTLIIYTEPQLPEGEYLFRVNMNMTPVITH
                     RGWITVNVSGKVNELKD"
     gene            complement(3274..4344)
                     /locus_tag="PK154_05360"
     CDS             complement(3274..4344)
                     /locus_tag="PK154_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10524.1"
                     /translation="MKGKGTTSGCMLLIAVVLTGSIMPGVSALSPQWVLPELKIDESS
                     ELEILNGELSPAEGKNLYSIPVGAIIQHSSDGITRVFTPEGTQIISAIDADSKKVPVP
                     SAGLLPVTHVHRIPSGSDIITQNNITKVYKDEVCILTVLGYGDTEKKLPYPSYEGWIE
                     QANNWSIDEIAQFIAYWDVPSSPSSQSDSVIFLFNAIEPSNGQGIVQPVLEWNRHAYR
                     DWTGAAWFVPYQQEGYYSEPIDVDVGDTIKGTLGWNPGLSQWNIIFKNEDTGISTMNW
                     SGCVGYQNDAVFCALEGYYVDGDSEVPGDTTFYDMRLKDLNLQNVEFNWEEDIDEVAE
                     GYLSGLDVQIHSDSMVTLHTAN"
     gene            4695..5438
                     /locus_tag="PK154_05365"
     CDS             4695..5438
                     /locus_tag="PK154_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW10525.1"
                     /translation="MTLWILFIGFCVLELVYIPLFGVDGTGVPVPRWQMRPSDLICHM
                     VHSVSPALVEPVQILLFWGIGFGAYFGFRTISRKTVLDNTARLRVFNTIQEYPGIHFN
                     ALREQTGMNRGTLRYHLSILSLTGKITSFQDGVFSRYVLSGMGMSSYDQIVISRFQNG
                     SDRKILSYLLDHPDTSQRDIAEALGVAPSVVSSRITKMYDEGVVAMERLGRSSQIALT
                     DNAVDVIRRIQGTGIHPDQISVPAPEIPG"
     gene            5613..6536
                     /locus_tag="PK154_05370"
     CDS             5613..6536
                     /locus_tag="PK154_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10526.1"
                     /translation="MSPDLKTEIPVCVLALAALISVITPCAAGIHIQASHDTVLAKGD
                     PLTITGDCYHNGSATLWGLGYSFLDSKVVNASPTGTIFWTLDSETTQTFHSGPFMIIV
                     QDPGQDRNYSISANVSAGICRMITADGIPLFETEAQADNISAALDLANLLENQIMGSG
                     TDDSCVIQTVYIEEPALHLSTVELGSALVVPAGEYVLVNGTMNMAPENLITARMYDTD
                     VIEETGLRIPVRPATVVATERGAWENTWEYTLDTAGLEPGEYLVEVGWNRSQVSGQNA
                     FLLQVIQPENRVERVLNGLLWRIPRGIFGFS"
     gene            6942..7115
                     /locus_tag="PK154_05375"
     CDS             6942..7115
                     /locus_tag="PK154_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10527.1"
                     /translation="MAARVAGMQERNTMEQLVVTREAVEAYERRTGFVGIGEFFLRKG
                     LFVLKEGETCRKK"
     gene            7243..7419
                     /locus_tag="PK154_05380"
     CDS             7243..7419
                     /locus_tag="PK154_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10528.1"
                     /translation="MPTAQHSTSPVPLYLLPQALAEEIKKYGDTIAEIRVRRTIGHNY
                     VLKVKHEKRGDRSD"
     gene            7412..7765
                     /locus_tag="PK154_05385"
     CDS             7412..7765
                     /locus_tag="PK154_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10529.1"
                     /translation="MTEPGTLSLSENEILRVEIDGKSYRIEAKEVRALLFYGRVVPII
                     QVQRKTSADGKDEGEVISIEGHTAINRAGKAVILYTRAGHFIIPLVSFQRVARGEAVS
                     APLFPLLPDWQDGSA"
     gene            7762..>8275
                     /locus_tag="PK154_05390"
     CDS             7762..>8275
                     /locus_tag="PK154_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10530.1"
                     /translation="MSSEVKRALAALFRDGGIVELRALTDHSVHSGYFDNFDTLAEKA
                     ANLDTLPEVAGIYVTLNAVDPALLSRRANRVKMNLGRKDPTTSDSDVISRRWLPIDLD
                     PVRPSGVSSTDEEHEAALAHAGRIRTWLGEQGFPDPVMADSGNGAHLLYPIDLPNDDE
                     STDLVKGCLAV"
BASE COUNT         1958 a         2168 c         2092 g         2057 t
ORIGIN      
        1 tgagggaccc agaatatcct ctcttctcgg attaaaaaag gtatggttat ctgaattttc
       61 gatctggcct gcaggggtgg cctgatgccc atggcacagc actccacctc gccggtccca
      121 ctctatctcc tcccccaggc actttcggaa gagatcaaga agtacggaga tacgatagca
      181 gagatcagga tccggcggac caccggccat aactatttcc ttaaggtcaa gcacgagagg
      241 cggggtgacc gtggtgactg aacccgggac gctctcactt ggcgagaagg agatccttcg
      301 tcctgtaatc atctatcggg taacaagaga aggggaacca taaaaatttg atgattttct
      361 atgaatccga tcacctaccg cagaaccttc cgaagtgcca gtgctgtgat acagccccca
      421 atcagtgtga tgtaggcgaa accgggcgac gatgtcggtg gtattgctcc gccttggttc
      481 agggaactgt ttacaggcga gtgagagtta ctcctgcccg tatcactcat tccagtctct
      541 cctgggcgag acgagagggt atgaagcacg acctcgcgtg gctccctgga ccagtcgtct
      601 cccgcaccgc gcgtatagat gatgtattcc cctgagatga ccggatgaga aagcccacgc
      661 cggaaaggag tgatctgggt cttttccccg gtgcgaaggt ccatcaccat aattgcactt
      721 ctgctgtccc ggaaatcgaa aggattcatg gactgcagcc atgcaacccg ctcatccgag
      781 atgggaggaa gatgcccctg gtgcacgctt ccctgcccga caacggtcct gacccgggaa
      841 gcgatggagt aatggatgat tgtatggatc ccatggatct cctctgtcca taccacatcg
      901 tcgccagaaa tatacggggt gctctttacc ggattttccg ttagcagacg ctcctctgtt
      961 ccgttcatca gatcgtagag gtaaagttcc ggatcatccg gaccatggga tcggtagtct
     1021 ttccacagta tgaaattacc tgaaatcata ggggtactct ggtggcccgg tgaaccggtc
     1081 gggaggtact ggttctcccc ggtcgtcaga ttgaagatga cgatctttcc cccgtcaacg
     1141 ctggagtggg agactccctc atgttcggtc cagacaaccc ggtccccatc aatcgaggga
     1201 ttctggctaa acctgagatc ggacactggt aggaatgcat catcctggtt ggtaagttga
     1261 gccagggatc ctgtagagat attcatcagc atgatccctt tttcgctgaa cacaacccgg
     1321 tcacgggaga ttctcgccat ttcaacctga tgtatgatgg gaatcgtgct gtctgaactc
     1381 atctgccctt gttctccgat tttaataagt tgttttttcg ttccctctgt aaggttatat
     1441 ccgtaaaacc cccgttcaga agcactcagg tatttattat cctggagcca gaaaagccgg
     1501 tctccttcca aggtactata gaacggttct cccgaaagag taaagagaac ggcttctgtt
     1561 ccaggtacgg attctgcgct gatagtccag ggaatagtga ccagacagat gacgattatc
     1621 aggaggccgc tggaacatcg atggttattc atggctgaaa aaatggttag gggaagctga
     1681 acaccgaagt gtcgtcatag tcctcggcat agtagggcgt aggaatatac ccccatgcat
     1741 ggacgtagta ctggccattc gcaggatata acttatcttt cgatgcggtg actgaccata
     1801 ccccctgttg tgaacttgcc gatgtcgaat ccacggtttg ccctgttccc cggttaatca
     1861 ggaacgcttc gacatagata taccagggag gttcctccag tcccgatgca gtacaggttc
     1921 ctccaaaacg gatggtcctc cagtcaaact tgtatccagt cgcactgaca gaaacagtgg
     1981 cacgatccag ggccggctgg tcatcgttgg tatcgttgtc ctgtcccggc tccgttgccc
     2041 accaggtcat cttctgaccg gccatattct tcctatatga atcggggatc ccgacaaggt
     2101 gttccgggtg gaccttctcc atatattcaa ggacagtaat gtccttgtca tagagctcat
     2161 tgattaggct caactgctct gcagtggcat ctgatcggac gactcctttc tcgtcttccg
     2221 cattcgctaa accgatcagt gcggatacaa gtatgaggac cagcatgaaa accgatcgca
     2281 tttttattcc atagagtttc atgattttgc tccttcacag gcttggtggc aaatgccaaa
     2341 ggaaggcccg gaaatgtgac agcgtaggta gtttttacgt tccttgtggc tgtcgtggga
     2401 tgagggtttt atcctcaccc tccatgggta atcggaccta ggtgcataaa cgatctctgt
     2461 gccagatcca gaactgtcag cagaatgatt cgcaatactg cacgtgacgg tattgatgat
     2521 tctctgtatc aatgattctc ccggatgagc acggtctgca tctcgatccc caacagacca
     2581 tgggtgtgat catattcacg attcattaaa acggaatcca tgcgttattc aggtgttttc
     2641 caggtagtgt gcgtataaag agaaaccata cctgaacaag aggatctccc cctcatattt
     2701 caggcaacgg tcgaggagtc atttaatctt tcagttcatt cacctttccg cttacattca
     2761 ccgtaatcca accccgatga gtaatgacgg gagtcatgtt catattgaca cggaataagt
     2821 actccccttc cgggagttgc ggttcggtgt atatgataag cgtggagacg tatgtcccgt
     2881 tgggcgatac gacatactcg gacggcgaca tgcttacatt caggtatcgc ggcatgggaa
     2941 tttcctcatc agaatttact gatttctctt ttaccgggac tatccgctgt atgaccagcc
     3001 cctgaccctt cgctctcgtc ttgagaatta tctgttcctc ttttatctcc cctgcatgta
     3061 tctctatcct gctaggatga aaccataccg gaatacccaa atgttgtttc cctggcgtca
     3121 catttggtgg ttcccatttt tcgttcctcc tatccattga tgagatgtct gtaacttggc
     3181 tggtaatgag cgataatacg ccgattacta ggataaaagt cgcaaagaac aaaaaaatta
     3241 gcgtggtttt ttccatccca ggtatctctc cgttcagttt gccgtgtgta atgtcaccat
     3301 cgaatcggaa tggatctgga catctaaccc ggaaagatac ccctctgcaa cttcatcaat
     3361 gtcttcttcc caattaaatt ccacattctg taaattcaga tctttcaacc gcatatcata
     3421 gaatgtcgtg tcacccggca cttctgagtc gccatcgaca tagtatcctt caagagcgca
     3481 aaagaccgca tcattctggt aaccaacaca tccagaccag ttcatagtcg aaatgccagt
     3541 gtcttcattc ttgaatatta tattccattg actgagacca ggattccagc ccaatgttcc
     3601 cttaatagta tcgcctacgt caacatcgat tggttcgcta taataaccct cctgttgata
     3661 tgggacaaac catgccgcgc cagtccagtc acgatacgca tgtctgttcc attcaaggac
     3721 cggttgcact attccctgcc cgtttgaggg ctctatagca ttgaagagaa aaatgaccga
     3781 gtcagactgc gaagacggtg atgagggtac atcccaatag gcgataaact gtgcgatctc
     3841 atcgatgctc caattatttg cttgttcaat ccatccctca taagaaggat acggcaattt
     3901 tttctcagtg tccccgtaac caagaacggt caaaatacag acctcatctt tgtagacttt
     3961 cgttatattg ttctgtgtga taatatccga tccacttgga atccgatgaa catgggtaac
     4021 tggtaataat ccggcagatg gaacaggcac cttttttgaa tcagcatcta tggcagatat
     4081 aatctgtgtc ccttcaggag tgaatacgcg tgtgattcca tctgacgaat gttgaattat
     4141 tgcaccaaca ggaatggaat agagattttt accttccgct ggactcaatt ctccatttaa
     4201 aatttcaagt tctgacgatt catcgatctt cagttcaggc agtacccatt ggggagaaag
     4261 cgcactcacc cccggcatta tactaccagt tagtactact gcgatgagaa gcatacatcc
     4321 actggttgtc ccttttcctt tcatcttttt ttcctccaat tctcttctga tatcatgatt
     4381 tcgctccttc cggttctcac gcgatacgtc gattcagagc ctgaattcat gacagcaggg
     4441 aaagcctctt cacttccgga atgttgtgtc gggatgaggg gcttgtcctc accctttgac
     4501 gataatctga ccccagtgta taaacgatct ctgtgccaga tccagaacta ttttcgcgac
     4561 gattcgcgat ccggcacaga aaggttatta gcgcctctga ccagatctga acaatgataa
     4621 tccgggtatc ttcagaaggg aaggagggta tcatggagaa ccgtcaatca catcgatttc
     4681 ataccgccct ttccatgacg ctatggattc tgtttatcgg attttgcgtc ctggaactgg
     4741 tctacatccc cctcttcggt gtcgatggca cgggggtgcc cgtcccccgg tggcagatga
     4801 ggccgtccga cctcatctgc catatggtcc attccgtatc tcccgctctt gtggagccgg
     4861 tacagatcct ccttttctgg ggaatcgggt ttggcgccta ttttggattc cgcactatct
     4921 cgagaaaaac tgtacttgac aataccgcaa ggctgcgggt cttcaatact atccaggaat
     4981 accccggaat ccacttcaat gcgctccggg agcagaccgg tatgaacagg gggacgcttc
     5041 gctaccacct ctccatcctg tcgctcaccg ggaagatcac ctccttccag gacggagtat
     5101 tctcgcggta tgttctgagc gggatgggga tgtcatcgta cgaccagatc gtgatatcca
     5161 gattccagaa cggatcggat cggaagatcc tctcctatct cctcgatcac ccggacacat
     5221 cccagcgtga tattgccgaa gcccttggtg tcgcgccttc ggttgtgagc agccggatca
     5281 caaagatgta tgatgagggg gttgttgcaa tggagcgtct tggcaggagc tcacagatag
     5341 ccctgacaga caatgctgtc gatgtgatca ggaggatcca gggtacagga atacatcctg
     5401 atcagatctc tgtcccggct cctgaaatcc cgggatgaaa gaactttcta tcggtgcttt
     5461 tcccccgcag aagacaatgg ctccccggga tatccttcat atcgaaacct ggaaaatcca
     5521 tcagggctgg tgtttgttcg tgttcctgca cagagatcgt ttaaaggatc ctattgatgt
     5581 ctcacagggc acctatgccc aggagataag acatgtcacc agacctgaaa acagaaattc
     5641 cagtttgtgt cctggcactt gctgcactga tatcggttat caccccctgt gccgcaggaa
     5701 ttcatatcca ggcgagtcac gacactgtgc ttgcgaaagg agaccccctc acaatcaccg
     5761 gggactgcta tcacaatgga tctgccaccc tgtgggggct aggatattca ttcctcgaca
     5821 gcaaggttgt caatgcgtcc ccgactggaa caatattctg gacattggat tcggagacga
     5881 cccaaacgtt ccatagtggc cccttcatga ttatagtcca ggacccggga caggaccgga
     5941 actactcgat ctcggcgaac gtgtcggccg gaatatgtcg tatgataacg gcggacggta
     6001 tacctctctt cgaaaccgag gctcaagccg ataatatctc cgctgctctg gatctcgcaa
     6061 atctcttgga gaaccagatc atgggaagcg gcacggacga ttcatgtgtg atccagacgg
     6121 tctatataga agagcccgca ttgcatcttt cgaccgtgga gttaggtagt gccctggtag
     6181 ttcccgcagg agagtacgtg ctggtgaacg gtaccatgaa catggcaccg gaaaacctga
     6241 taacggcgcg gatgtatgat acggatgtaa tcgaagagac cggtctgcgc atcccggtcc
     6301 gacctgccac ggtcgttgcg acggagaggg gggcttggga aaatacctgg gaatataccc
     6361 tcgataccgc aggactggaa ccaggggaat acctggtcga ggtcggatgg aatcgatcgc
     6421 aagtatcagg acagaatgcc ttccttctcc aggtcataca accggagaac cgggtcgaaa
     6481 gagtgttgaa tggattattg tggcgaattc cacgaggaat ttttgggttc tcataatgtg
     6541 agcgattata aatattcttg tccatcttat aggatatcac ctccggagga atctggattg
     6601 cgatggacaa ggttcatatt tttatatcaa ttttcctggg gttctgcact tccgggtgca
     6661 tctcggattt ccccccatcg atgaacactt ccccacaggt tacacctgga ggagatatcc
     6721 cgattccccc ggtaaccatt caaaagattg aggtccccgg ccccgggacc gcgaattatt
     6781 ctatccctgt cctgcttacc gatctcgatt gggagcttgc gagtgagtgc gggtggatct
     6841 cgaggtgatt ccctttatgt gatacagtca ggcttatcct gacccacttt caggagctcc
     6901 cgttcaccct tatttcaccc gtttgccatg ctggtgtgca tgtggcggca cgggttgccg
     6961 gcatgcagga acgaaatacc atggaacaac tcgtggtcac acgggaagca gtcgaggcct
     7021 atgaacgccg gaccgggttc gtgggcatcg gggagttctt tctgcggaaa ggactctttg
     7081 tcctgaagga gggtgagaca tgcaggaaaa agtagtgggc cccccaaaat cagaagcatc
     7141 ttccacaatg attcaacttg agggacccag aatatcctcg cttctcggat taaaaaaggt
     7201 atggttatct gaattttcga tctggcccgc agaggtggcc tgatgcccac ggcacagcat
     7261 tccacttcgc cggtcccgct ctatctcctc cctcaggcac tagcggaaga gatcaagaaa
     7321 tacggcgata cgatagcaga gatcagggtc cggcggacca tcggccacaa ctatgtccta
     7381 aaggtcaagc acgagaagcg gggtgaccgc agtgactgaa cccgggacgc tctcgctcag
     7441 tgagaatgaa atccttcgcg tggagataga tggtaagtcc taccggatcg aagcaaagga
     7501 ggtccgggcc cttctcttct acggccgggt cgtgccgatc atccaggtcc agcggaagac
     7561 atctgcagat ggaaaggatg agggcgaggt catctcgatc gagggacaca ctgccatcaa
     7621 ccgggcaggg aaagcggtga tcctttacac gcgggccggg cacttcatca tcccgctcgt
     7681 cagtttccag cgggtcgccc ggggagaagc ggtctcggca ccgctcttcc ccctcctccc
     7741 ggactggcag gatggatcgg catgagcagc gaggtcaaga gggccctcgc ggcccttttc
     7801 cgggatggcg ggattgtgga gctacgggcc ctgaccgacc attcggtcca tagcgggtat
     7861 ttcgacaact tcgatacgct agctgagaag gcagcaaacc tggacacgct gccggaagtc
     7921 gccgggatct atgtcacatt gaacgcggtt gacccggccc tgctctcccg gagagcgaac
     7981 cgggtgaaga tgaacttggg tcggaaggat ccgaccacca gcgactctga cgtaatcagc
     8041 aggcgctggc tccccatcga cctggacccg gtacggccaa gcggagtctc atcgaccgat
     8101 gaggaacacg aggcagctct tgctcatgcc gggcggatcc ggacctggct tggggagcag
     8161 gggttcccgg atcctgtcat ggccgactcc gggaacgggg cccacctgct ctacccgatc
     8221 gacctgccga acgatgacga atcaacggac ctggtgaagg gatgccttgc tgttc
//
LOCUS       DAOOCO010000072         8237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8196_length_8237_cov_4.663285, whole genome shotgun sequence.
ACCESSION   DAOOCO010000072 DAOOCO010000000
VERSION     DAOOCO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_8237_cov_4.663285"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK154_05395"
     CDS             <1..420
                     /locus_tag="PK154_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10531.1"
                     /translation="KIDASRGVEFLCGCLKDFDPNIRRLAAQILGDTGDPRAVESLID
                     QLKEPHMREDSAEALGKIGDSRAIEPLISSLKDSDPKVRKRAANSLVSFYHSGKLDPE
                     KQKMILANKSVIQAPHTDNTKQSDCSVHEDSGIGISF"
     gene            1302..2030
                     /locus_tag="PK154_05400"
     CDS             1302..2030
                     /locus_tag="PK154_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW10532.1"
                     /translation="MDQLPVPFRTNLISRQADLAALRAYPAENLAEIILEVGFQCDCC
                     SRCCTRDFNGHILLLDTDVRRIRSFAPESLEPVPVLDFCDQHGMYYASGYTLRSQGDS
                     KGSCYFLSERRCRIYENRPSICGLYPYMLHREPDDQGTVDWRQISGLNQHGGYNVKIS
                     EDEAHSLALEIKAFEEAVLSREISYLACTGRYFLDNGLRNVRKRFDDGLRSAREGGAI
                     EVMVFSDGGYDRWVIREGISIQMH"
     gene            complement(2083..2499)
                     /locus_tag="PK154_05405"
     CDS             complement(2083..2499)
                     /locus_tag="PK154_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HPW10533.1"
                     /translation="MGIFDKLKGSKTIQLTPKSALVAAAISVIASDGVLDEAELRDLA
                     KIARGDKSAVNDAVQILEGNSIQETMELVAKCLNEKQRMAAMAILLDLAMADGILAGN
                     EQKILQQYMEKFGISETAIKPLIDTIALKNDFSVFS"
     gene            complement(2606..3085)
                     /locus_tag="PK154_05410"
     CDS             complement(2606..3085)
                     /locus_tag="PK154_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW10534.1"
                     /translation="MQQHGNVTIRPARRDDAPALYDNNRATALETEGRILDPIVACKG
                     VIELFDNPSRGFYVVAELEDRVIGQCMITYEWSDWRCGNFWWVQSVYVLPEYRKMGIF
                     TQIFREIYRLAREKSQVAGLRLYVDRDNTTAMKAYGSLGMHEARYVLFEREFRDSQR"
     gene            3258..4040
                     /locus_tag="PK154_05415"
     CDS             3258..4040
                     /locus_tag="PK154_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10535.1"
                     /translation="MKSMKVISFLAVITAILVLCAGCTQTGTEKPSPEVKVYIVGIDG
                     QYPPFSYVDSSGNATGFDVDSMRWIAEKKGLAIEFQPIAWDGIIPALQAKKIDLIYAG
                     MTITDERKEKVNFSTPYWVVNQDVVANKGSNVTVEDVLSGKAIIGTQRGCTAAMWIEE
                     NLIGKGLMPQNNLKLYDNTPLAVNDLESGRIDAVMYDDLSLKDMIQGKEIEKIGSVET
                     KEEFGIAVRKEDTELLSTLNEGLEALMNDPYWQELISKYNMK"
     gene            4182..5312
                     /locus_tag="PK154_05420"
     CDS             4182..5312
                     /locus_tag="PK154_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW10536.1"
                     /translation="MKFPISLPSFRTIDPVYFGLGTAVLFGCGAPLAKLALDGVSPMI
                     LAGLLYIGSGAGLLLYLCLFSFSRPHNSRNESALKKTDIPWLSGVVICGGFLAPFTLM
                     VSLQFLAPASVALLLNFEVVMTTVLAALAFHEAIGRRVWTALLCITTACVLLTYNPGA
                     VTSISLPAMGVLLACTFWALDNNFSRNISIRDPVIIVIIKGFGGGILSVFAGLLLGET
                     FPGGSSTLTALCLGFVCYGGLASVFFLMALRGLGSARSGALLAVSPLFGGLLSLLLFS
                     GQFTTLFFIALPVMATGIILLLTEEHVHVHTHHHLVHEHRHGHDDLHHMHVHPTGNAE
                     QGDGAEHCHCHTHHEMTHAHPHRPDIHHRHPHDERGGGQRKD"
     gene            complement(5315..5980)
                     /locus_tag="PK154_05425"
     CDS             complement(5315..5980)
                     /locus_tag="PK154_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPW10537.1"
                     /translation="MEPAGFITDILIPALSQGLVVTLQLIVLSAPFGLLLGIGISLGR
                     QYGGKNLSNICKLYVIFIKGCPLLLLLFILYFGLPSVGITLTAFAASVIGFILCNAAY
                     NSEYIRGAILSVKDGQTIAAQALGMTKWQVIREVVLPQALRRAIPGLSNEFIYLIKYS
                     SLAYMLTVIELSGAGKLVATKYFAYNEVFIAVGIVYLALVTFTTVAVHYLEKRVAVPG
                     MIH"
     gene            complement(5981..6742)
                     /locus_tag="PK154_05430"
     CDS             complement(5981..6742)
                     /locus_tag="PK154_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPW10538.1"
                     /translation="MKDDAFVLRVEDIHKKFGTNEVLKGVSFNVRKGETIVFIGPSGT
                     GKSTLLRCINQLTIPEKGRVWLHDDEVTNSGPKISYFRQKIGMVFQNFYLFDHLTAVR
                     NVEIALLKVKKMDPESARRKALYELEQVGMKGWADHYPAELSGGQAQRVSIARALAMD
                     PEVILFDEPTSALDPELTREVLEVMKKLAKSGMTMLVVSHEMGFAASVANQIIFMENG
                     NIVEQGPPDALLYDPAYVRTRKFVGQFADLTQRKD"
     gene            complement(6747..7421)
                     /locus_tag="PK154_05435"
     CDS             complement(6747..7421)
                     /locus_tag="PK154_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPW10539.1"
                     /translation="MDVTSILAEWTPYLIGGISLTLGLVAIALGIGVALGLPFALVQV
                     YGPRPAKAAISAYAWFFRGLPVLVLLFLFYFGIFPAMGLDLPAFFVGAAVLGLRGAAY
                     QSQIFRGAIQSINEGQMIAALSLGMTRLTAIKSIILPQSLRIALPGWSNEYPEILTDS
                     AICYAIGVAELLTRTSQIVAVTYVTMPIYLCCALIFILLNYAGMRVIHVVEKRVAIPG
                     FGAGAG"
     gene            complement(7535..>8237)
                     /locus_tag="PK154_05440"
     CDS             complement(7535..>8237)
                     /locus_tag="PK154_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10540.1"
                     /translation="TSTTPQAKTYVVGIDAEYPPYSYIDPDGNVTGFDVESMRWIAAK
                     EGFNVTFQPTAWDGIIPALQAGKIDIIYSGMTITDERKEKVSFSIPYLKINQSVAVHK
                     EGNYTMDDFMAGGLIIGAQRGTTGAIWVEENLIETRKMPAENLKVYDNFPLVVTDLAF
                     KRIDAAIYDRPPLLDAIADKPLVIIDPQIDTHEEYGVAVRKDDTLMLDAINKGLSSLM
                     QDPYWEELKQKYGLI"
BASE COUNT         2030 a         2061 c         1999 g         2147 t
ORIGIN      
        1 aaaatcgatg ctagcagagg tgtggaattc ttatgtggct gccttaagga ttttgatccg
       61 aatatacgcc gtttagcagc tcaaatatta ggagacacag gagatcccag agctgtcgaa
      121 tcgttgatag atcaactcaa agagccccat atgcgtgaag atagcgctga agcattgggt
      181 aaaattggag attcaagagc tattgagccg ttgatctcct ctcttaaaga ttccgatccg
      241 aaagtacgta aaagggctgc gaattcacta gtatccttct atcatagcgg aaaattagat
      301 ccggagaagc aaaagatgat tcttgcaaat aaatcagtta ttcaagcacc acataccgac
      361 aatactaaac aaagtgattg tagtgttcat gaggatagtg gcattgggat ttctttttaa
      421 ttttgtaact gttcagatac tctgtgagat cttctatgaa gtcaaactat cggatatatc
      481 tgtgggggaa cacctagaat ttcatttcct cgaccgataa tattattcca gatcccaaat
      541 acaaccgtgc ctccacctcg tttgccacgc atactgccat ttcgatgacc tttacaagaa
      601 tacttggaaa atatctaact gagatatctt tccatcgact cttcatgtat ttcctcagtt
      661 ctacaatgtt aattcgttcg ggcactcttg gttagtaaaa tgttggaagc ctttattagg
      721 gaattaccaa ataaaatcga ttaccattaa aggtttttca gtttcacact cattccgcgc
      781 ggatgcaaat cggagcgatt tttacgctct gtctggacgc cgggctgagg catacttctc
      841 aaaagacgca tagaatctat ttattttaat ttttacgttc taatcctcaa tctccataaa
      901 acggctcttc ctccattacc ctcattcttg aaatcccagg tccagtcggt ccgatgttta
      961 tgaaagacac gagatcattt tatctgtcag catttgtggc aagacgaagt gagaatcttt
     1021 cattcgacag ggatcgtgct gtatgcaatt ccaggtatcc agatcctctt aataaatcag
     1081 agaagctatc ggactgggca tctccagtcc agctttgccc cttgaattga tctccgtatc
     1141 cttcaccata tgcagattgg ttcattccag gatgttagat gatggatttt aatgcctcga
     1201 ttgtcagaat ataccctcac tggctgactc ctttctcatg tttggatatc atgacaagaa
     1261 cgctgatttt ttgccttttg gcggggatta cctgtaaacg tatggatcag ctcccagtcc
     1321 cgtttcggac taatttgata tcacggcagg cggacctggc agctctgcgt gcatatcctg
     1381 ctgaaaatct cgccgaaatc atattagaag ttggcttcca gtgtgactgt tgctcacggt
     1441 gctgcacaag ggatttcaat ggacatatac ttctcctgga tacggatgtc aggaggatcc
     1501 ggtcatttgc acccgaatct ctggaacccg ttccggtcct tgatttttgc gaccagcatg
     1561 ggatgtacta tgcttccggg tataccctcc ggtcgcaggg agattcgaag ggttcatgtt
     1621 atttcctcag tgaacgaaga tgcaggattt atgaaaatag gccctcgata tgcggactgt
     1681 atccctacat gcttcacagg gagccggacg accagggcac tgttgactgg cgccagatca
     1741 gcggactcaa tcaacacggc gggtataatg tgaaaatttc cgaagatgag gcacattctc
     1801 tcgcacttga gataaaggcc tttgaagagg ctgtcctctc ccgcgagatt tcctatctcg
     1861 catgcacagg gcgatatttt ctcgacaatg gactgagaaa cgtcaggaaa cggtttgatg
     1921 atggcttgcg atctgccagg gaaggaggag cgatcgaggt catggttttc tcagatgggg
     1981 ggtatgatcg gtgggtgatc agagaaggga tttcaataca aatgcactag ataactcctg
     2041 atcgacatta gataactcct gatcggacat cgtaaaaaag aattaagaga agactgaaaa
     2101 atcgtttttt agagcgattg tgtcgatcag gggctttatt gctgtttctg aaatgccgaa
     2161 tttctccatg tactgctgga ggatcttctg ttcattcccg gcgaggatac catcagccat
     2221 tgcaaggtca agcaggatcg ccatggcagc catccgctgc ttctcgttga gacactttgc
     2281 aacaagttcc attgtttcct ggatagaatt gccctccagt atctggactg catcgttcac
     2341 ggcactttta tctcctctgg cgatcttcgc aagatcccta agctccgcct catcaagaac
     2401 tccgtcagat gcaataactg aaattgcagc tgccacaaga gcagatttgg gcgtcagctg
     2461 gatagtttta ctccctttta atttgtcaaa gattcccatt atttcacctg gtttgaagga
     2521 tcagtattgc tgctgaagca tattttcttt tttggtcttg gtgcgtgtta atctccaaga
     2581 cagtgaaaat ggaatcatgg tattactatc tctgggaatc cctgaattca cgttcaaaga
     2641 ggacatagcg agcctcatgc atacccagtg aaccataagc tttcatcgca gtggtattgt
     2701 cacgatcaac gtagagcctg agaccggcca cttgggattt ttctcttgcc agcctgtata
     2761 tttccctgaa aatctgagtg aatattccca tttttcggta ttctgggaga acatagacac
     2821 tctgcaccca ccaaaaattt ccacacctcc agtcactcca ttcgtaggtg atcatgcact
     2881 ggccgatgac ccgatcctcc agttctgcaa caacatagaa tccgcgtgag gggttgtcga
     2941 agagttcaat aacccctttg catgcgacta tcggatcaag gatgcgaccc tcggtctcca
     3001 gggcagttgc cctgttgttg tcatagagtg ctggggcgtc atcacgacgt gcggggcgta
     3061 tcgtaacatt tccatgctgc tgcacacacc atggtaaaat gtccagtggc atgagtctta
     3121 cgttgtagtt cttgggaccg gtttgtggta atatgctaat cccccattga accctcccta
     3181 ctgcagtcaa accgttacaa acaatggatc gagccaatat gaaacgcata tattcattat
     3241 cgttgaaacg ttcttgcatg aaatcaatga aagttatctc atttctggca gtaatcactg
     3301 ccatcctggt actctgtgcc ggatgtaccc agaccgggac tgagaaacca tcaccggaag
     3361 taaaagtata catcgttggc attgatgggc agtatccccc cttcagttac gtggattcaa
     3421 gcgggaatgc caccgggttt gacgtcgatt ccatgcgctg gatcgccgag aagaaagggc
     3481 tggcgattga gttccagcct atcgcctggg atggcatcat ccctgcactg caagctaaga
     3541 agatcgacct catttatgca gggatgacga tcaccgatga gcggaaggag aaggtgaact
     3601 tcagcacccc ctactgggtt gtgaaccagg atgtggtggc aaacaaggga tccaacgtaa
     3661 ccgtagagga tgtgctttcg ggaaaagcga tcattggaac ccagcgtggc tgcacagcag
     3721 ccatgtggat cgaggagaat ctcatcggga agggtctgat gccgcagaac aacctgaagc
     3781 tctatgataa cacgccgctt gccgtcaatg atcttgaatc aggaagaatc gacgcggtta
     3841 tgtatgatga tctctccctg aaagacatga tccagggcaa ggagatcgaa aagataggct
     3901 cggtcgagac aaaagaagag tttggtatcg ccgtcaggaa agaggacacc gaacttctgt
     3961 ccaccctgaa tgaagggctg gaagcgctga tgaacgatcc ttactggcag gaactgatca
     4021 gtaagtacaa tatgaagtaa cccagagtgg gggcattgga agggctgaag gaaccttcta
     4081 tccctggaag ggaacagatc cggatcctgg gatttgatcc cgaatcccct ccctggtctt
     4141 tggatttttc atcatactca tcctgattat caggcgaatg catgaaattc cccatctccc
     4201 tgccatcgtt ccggaccatt gacccggtct atttcggcct tggcaccgcg gtacttttcg
     4261 ggtgcggagc tccccttgca aagctggcgc ttgacggtgt cagcccgatg atccttgccg
     4321 gactcctcta tatcggttcc ggtgcaggtc ttctgcttta tctctgtctg ttctcctttt
     4381 cccggccgca taattcacgg aatgaatccg cgctcaaaaa aaccgatatt ccctggctat
     4441 cgggtgtggt gatctgcggg ggattccttg cgccattcac ccttatggtg agcctccagt
     4501 tcctggcccc agcctctgta gcgctcctcc tcaacttcga agtggtgatg accaccgttc
     4561 ttgccgcgct tgctttccat gaagcgatag gaagacgggt atggacagcc ctcctctgca
     4621 tcaccacagc atgcgtcctg ctaacctata atccgggggc cgtgaccagc atctcgctcc
     4681 ctgcaatggg ggtcctcctt gcctgcacct tctgggctct cgataataat ttctcgagga
     4741 atatctcgat acgcgatccg gtcataattg tcatcatcaa aggattcggc gggggtattc
     4801 tctcggtttt tgcaggactg ctgcttggag agacatttcc tggaggatca tccactctta
     4861 cggcgttatg cctgggattc gtctgttatg gtggccttgc cagtgtcttc ttcctcatgg
     4921 ccttgagggg tcttggcagt gccagatctg gtgcacttct cgccgtctcc cccctgtttg
     4981 gtgggctcct ctcccttctc cttttctcag gtcagtttac cacactgttt ttcattgcgc
     5041 ttccggtaat ggcaacaggc atcatactcc tgctcaccga agagcatgtt catgtccata
     5101 ctcatcatca cctcgtacat gaacatcgcc atggtcatga tgatcttcac catatgcatg
     5161 tacatcctac ggggaatgca gagcaaggcg atggagctga acattgccat tgccataccc
     5221 accatgaaat gacccacgct catccccacc ggcccgatat ccaccaccgc catcctcatg
     5281 atgaacgtgg gggcggtcaa agaaaggatt agttttagtg aatcataccg gggacggcca
     5341 ctctcttttc cagataatgg actgcaacag tggtgaacgt aaccagggca aggtagacga
     5401 ttccaaccgc gataaatacc tcgttatagg caaaatactt ggtagccacc agcttcccag
     5461 ccccagaaag ttctattacc gtaagcatgt aggctagtga agaatacttg atcaggtaga
     5521 tgaactcatt cgacaatccc ggaatagccc ttcgcagagc ctgggggagg accacctcgc
     5581 gaatcacctg ccacttcgtc atgccaagtg cctgtgcagc aatcgtctgg ccatctttta
     5641 ccgaaagaat ggcccccctg atatattccg agttatatgc ggcattgcag aggatgaacc
     5701 ctatgaccga tgcggcaaag gcagtcaggg tgatcccgac cgagggaagt ccgaaataga
     5761 gaatgaacag gagaagcaga agggggcagc ccttgataaa aataacatac agcttgcaga
     5821 tgttggagag gttttttcca ccatactgtc ttccgagcga gatcccaatc ccgagaagca
     5881 ggccaaatgg tgcggagaga acaatcagct gcagggttac cacgagcccc tgtgagaggg
     5941 ctgggatcag gatatcagtg ataaaccctg caggttccat ctaatccttc ctttgggtaa
     6001 ggtcagcaaa ctggccgacg aattttcttg ttctcacata agccgggtca tagagcagtg
     6061 catcgggagg accttgttcg acaatgttcc cgttttccat gaagatgatc tggtttgcaa
     6121 cggaggcagc aaatcccatc tcatgggaga ctaccagcat ggtcatcccc gattttgcca
     6181 gtttcttcat gacttcaagc acttctctcg tcaactcagg atccagggca gaggtgggtt
     6241 catcaaagag aatgacttcc ggatccatgg caagtgcccg ggcgatggat accctctggg
     6301 cctgtccccc ggagagctcg gcggggtagt ggtctgccca gcccttcatg ccaacctgtt
     6361 cgagctcgta cagcgctttt ctccgggcac tttccgggtc catctttttg accttgagaa
     6421 gggctatctc cacattcctg actgcggtca gatggtcgaa gaggtagaag ttctggaaca
     6481 ccatccctat cttctgcctg aaataggaga tcttaggtcc tgaattggtc acttcatcat
     6541 cgtggagcca gactctccct ttttctggga tggtcaactg gttgatacag cggagcaggg
     6601 tgctcttgcc cgttccggaa ggcccgataa aaacgatcgt ctcccctttc ctgacgttga
     6661 aggagacccc tttgagcacc tcgtttgtcc cgaatttttt atggatatcc tccacccgga
     6721 ggacaaatgc atcatctttc attggactat cctgcccctg ccccgaagcc cgggatggca
     6781 actctttttt cgaccacatg gatgactctc atgccggcat aattgaggag gataaagatc
     6841 agggcgcaac acaggtaaat cggcatggtg acataggtta ccgcaacgat ctgcgatgtc
     6901 cttgtcagca gctctgctac gccaatagca taacatatcg ctgaatcagt gagtatctcg
     6961 ggatattcat tcgaccaccc tgggagtgca atccggagtg actgggggag gatgatggat
     7021 ttgatcgccg taagcctggt catcccgagc gaaagtgctg caatcatctg gccctcattg
     7081 atggattgga tagcaccccg gaatatctgg gactggtatg ctgcaccacg gagaccgagc
     7141 actgccgcac cgacgaaaaa agcaggaagg tcaagcccca tcgcagggaa tatcccaaag
     7201 taaaagagga acaggaggac gagcacagga agtcccctga agaaccatgc ataggcactt
     7261 atcgctgcct ttgcagggcg gggtccatag acctgcacga gtgcgaaagg caggccaagg
     7321 gcaacaccaa ttccaagggc tattgccacg agcccgagtg tgagggaaat ccccccgatc
     7381 aggtagggcg tccattccgc gaggattgac gtgacatcca taagggtatc ccgcgatcag
     7441 ggttctgtat accaagactc gttcacaaat gcttcccccc atctgtcttt agaggcgaat
     7501 cgataaagaa aatgaaatgg gattttgcgt tgtttcatat tagcccgtat ttctgcttca
     7561 gttcctccca gtaggggtcc tgcatcaggg aggagagccc cttgttgata gcgtccagca
     7621 ttagtgtgtc atccttcctg accgctacac cgtattcctc atgggtatcg atttgggggt
     7681 cgataatcac cagtggtttg tccgcaattg catcaaggag gggaggacgg tcatagatcg
     7741 cagcatcaat tctcttgaaa gcaagatcgg ttactacgag cgggaaattg tcgtatacct
     7801 tgaggttttc agcgggcatc ttcctagttt caatcaggtt ctcttctacc cagattgcac
     7861 cggttgtgcc ccgctgcgca ccaataataa gtccaccggc catgaaatca tccatggtat
     7921 agttcccttc tttgtgcacg gcaacggact ggttgatctt cagataggga atactgaaac
     7981 tgaccttttc tttccgctca tcggtaatgg tcatccctga ataaataata tcaatcttcc
     8041 cggcctggag agcagggata attccatccc atgccgttgg ctggaatgtc acattgaaac
     8101 cttccttagc cgcgatccat ctcatggact ccacgtcaaa tccggtcaca ttcccatcgg
     8161 gatcgatata gctgtacggg ggatactctg catcgatgcc caccacatac gtttttgcct
     8221 ggggggtggt gctggtg
//
LOCUS       DAOOCO010000073         8212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8230_length_8212_cov_4.629030, whole genome shotgun sequence.
ACCESSION   DAOOCO010000073 DAOOCO010000000
VERSION     DAOOCO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_8212_cov_4.629030"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK154_05445"
     CDS             <1..323
                     /locus_tag="PK154_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10541.1"
                     /translation="SIVGRAVQNGGLDRHHLDEASVSITGSPLPGSMIQDTDIMDALD
                     VEKSINLKIAPGSPSSFSVKRAIDERKKRLDDDRHRTDQLAMASKEAIDNLIFETRRV
                     ALEQ"
     gene            313..1542
                     /gene="gatD"
                     /locus_tag="PK154_05450"
     CDS             313..1542
                     /gene="gatD"
                     /locus_tag="PK154_05450"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPW10542.1"
                     /translation="MNNEFHPGDIVLCRHGNTDRSGTFITMRDGMATIKLDSGYNIGV
                     SLDEICLVKRPDITSSSPVAVTQNEALPQLSIVSTGGTIASRIDYRTGAVTSQFDAED
                     IIRAIPRLVTIARYRARKLYTILSENMNPAIWMELAEAVFQEIRNGSSGIIVTHGTDT
                     MAYSAAALSFMIDTPVPVIFVGSQRSADRPSSDNTVNALCSASAAVSDLGEVVVVMHG
                     TMSDDSCHIHRGTRVRKMHTSRRSAFESIGIPPIGSVSYPSLEVHLEPAAIRRGTQEP
                     SLAASLEERCGLLHFYPGMPPEAFTSFSDYKGLIIAGTGLGHVSTLLIPTIKELVDDG
                     TMVVMTSQCLHGRVCDRVYDTGRDLIKAGAIEGGDILPEVALVKQMWVLGNEKDPERA
                     AVMMKSCLKGECGGRSL"
     gene            1542..3398
                     /gene="gatE"
                     /locus_tag="PK154_05455"
     CDS             1542..3398
                     /gene="gatE"
                     /locus_tag="PK154_05455"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPW10543.1"
                     /translation="MDYGKVGLKAGIEIHQQLNTAQKLFCNCPTTLRTIDEHDKEFFR
                     YLRATESELGEIDAAAKEEMKQERMFCYFGYDTTCLVEYDEEPPAPLNHEALELACII
                     SRLFEMTPVEQIHTMRKLVIDGSNTSGFQRTALIAVDGILPNGGKIETICLEEEAAQR
                     VDGDTFSLDRLGIPLVEITTSPCMKTPDEVREIAGYLGMVLRSTGRVKRGLGTIRQDI
                     NISIAEGARVEIKGVQELDLIAEVVQREVLRQKNLVDIRNELLSRQATVITEPVDVTK
                     IFYASSSTILKKAHCILAVRLPGFAGLVGREIQPGRRLGSEFSDYAKKCGVGGIFHTD
                     ELPAYGITRDEVDRLFVQISAGSDECIVLVAADGKKAHCAAMQVIKRAETALTGVPEE
                     TRKMLEGGSTSYMRPLPGAARMYPETDVFPVRIEPTWYENLPVPELLVEKAERFVREL
                     HIDPALASQLASSDQVFLFERAVSEGIAPSLAARTLLSSRKELARGGSNISCLSDENI
                     LMVLKAVEEGRAAKEAVPVILEKVGAGTSVSEAITECAPAVSRSRLEEIVRMVVRERR
                     DFVREKGMGSLGPLMGIVMAEVRGSVDGRVVSQVLKEEISRELSPNTPGDKV"
     gene            3435..3758
                     /locus_tag="PK154_05460"
     CDS             3435..3758
                     /locus_tag="PK154_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HPW10544.1"
                     /translation="MGKTGSVQWVQVKGVKGQIRLVPKAEGELKRAGPNQRFKAGASL
                     KKLEAATGDGRGGPRRGGSRGGRGGRGARGGRSEQGPAANPMIRRPMRRAKASIMGAK
                     QKSAR"
     gene            3844..4200
                     /locus_tag="PK154_05465"
     CDS             3844..4200
                     /locus_tag="PK154_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10545.1"
                     /translation="MDLKTAIKTYQFAERAKSELIICSQLLNALAGFPEQERPGAKRM
                     LLLVIESVRSELEFAFRDTERAEFKKGIGFLNEAISLSESDQYGSAVLKLSEAISAAT
                     TAAQGAWKVLADNGLL"
     gene            4187..4726
                     /locus_tag="PK154_05470"
     CDS             4187..4726
                     /locus_tag="PK154_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW10546.1"
                     /translation="MDSSEYLEFLNTRCSVREYSDEDISDEEITYLLNCVTTAPSAGN
                     LEAWDIVLVRDAGRKEGLSDAAMGQLQVLNAPAVFVICANYVRSMSRYGDRGILYAVQ
                     DATIACTYMMLAAHALQIRTCWVGAFDEETVREIVSLPPHIRPIALLCIGKGRLPSQL
                     TERMAVSEHVHIEEWYHNA"
     gene            4719..5057
                     /locus_tag="PK154_05475"
     CDS             4719..5057
                     /locus_tag="PK154_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HPW10547.1"
                     /translation="MPDYVVTLESAWVIKDVKSLDDAVGIAISEAGKRLNPSAKYVEI
                     EAGMLECPFCEGELNSALIVANTALVGLLLEMKVFRADSQDHAAKIAKSVVGKALRDV
                     PLLVKEVCEL"
     gene            5054..5935
                     /locus_tag="PK154_05480"
     CDS             5054..5935
                     /locus_tag="PK154_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPW10548.1"
                     /translation="MIHIVGHTAVDHISRVSALPAKNGSSTILHRKVLFGGGAANIAT
                     GIARLGGEVTLVSAVGSDFPGSAYETWLKENGVNTKFYTVSGKNTPTAFMFTDPRGDQ
                     ITFFEWGASEIFKVSDPPAFPFVHMATADPEFNVKVAENAGFSSFDPGQDLHRYSAEQ
                     LYSIISKISLLFANQHEVESMCRIMDLSLDDLTKMVPMAVFTSGADGSTLYQGGKSIH
                     IPAIPVRLADPTGAGDAYRAGFLTAYTIGLPPVVCCRIGTVTASYAVEVEGCQTNLAD
                     WNEMRVRYEQFFGSTDI"
     gene            5989..7728
                     /locus_tag="PK154_05485"
     CDS             5989..7728
                     /locus_tag="PK154_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HPW10549.1"
                     /translation="MGFVSGDVKIERLSRFFFVAPSWPVSLAIIIILGIVIDGASLRS
                     GQHIRFFGSLCFTLPALAGFLITKPLVRLLSGQITLNRSALLSLTCTVFLIIISLAGM
                     VVSIPLFPFSYAIALGFIFGIRLVVLAAIADYRLIRMTLPAFVQSGVGILIGMVLFTP
                     PFGLFATLSTIVFGIGFVLLIWALDRPLYRAFHIHGLNFLNTFLAHITDGSKTMEDFF
                     HEIGEEVYVPQVNLLFRPENGGGVLFTVPNIHPGPLGEIGGGNLPNYLQSSFSELVMV
                     SHGTATHDFNLVAEDEIEKIIRAIRKAESALVFSDKASRSHRYQYGSVSVLYQVFNDT
                     LLIISTRSPEKTEDIDLGVGMAIMAEGHRAFRHVAFVDAHNCFTGDISTVQPGTLSAL
                     EYHSAALHAIEDGMRLEQSRLRFGSAQVIPPFSRKEGFGDQGIEVLVIEAGGQKTAYV
                     LIDGNNVLAGVRDNLRTSVLNLVEEAEIMTTDSHVVNILSGKNPVGYHVDPELILPYL
                     DQAVKEAINNLSWGEAAGSTTLCERVRVFGSQRMVQMASTVNAMILFIPPLSAAVLLL
                     AFLLSLMLYMIII"
BASE COUNT         2017 a         1992 c         2218 g         1985 t
ORIGIN      
        1 atagtattgt cgggagagcg gtccagaacg ggggtctgga caggcaccac cttgatgaag
       61 catcggtaag catcaccggc tctccgttgc caggcagtat gattcaggat acggatatca
      121 tggatgcact cgatgttgaa aagagtatca atctgaaaat cgcccctgga agtccctcat
      181 ccttctctgt caagagagcc attgatgaac ggaaaaagcg gcttgatgac gatcggcaca
      241 ggactgatca gctggcaatg gcatcgaaag aggcgattga caacctcatc ttcgaaacga
      301 ggagggtcgc ccttgaacaa tgagttccat ccaggggata tcgtgctctg tcggcatggt
      361 aacaccgatc gttccgggac atttatcacc atgcgggatg ggatggcgac gataaagctg
      421 gacagcggct acaatatagg agtatcactc gatgagatct gccttgtaaa gcgccctgat
      481 atcacttcgt catcacccgt tgcggtgacc cagaatgaag cgctccctca gctctctatt
      541 gtatcgaccg gaggaacgat cgcaagcagg atcgattacc ggactggggc agttaccagc
      601 cagttcgacg ctgaagatat catcagggca ataccaaggc ttgtcaccat cgcccgctac
      661 cgggccagga agctgtacac gatattatca gagaacatga atccggctat ctggatggaa
      721 cttgcggaag cagtcttcca ggagatcagg aatggttcgt ccgggatcat cgtcacccat
      781 ggaactgaca cgatggcgta cagtgctgca gcgttgagct tcatgatcga caccccggtc
      841 cctgttattt ttgtgggttc acagcggtcg gcggatcgcc cgagcagcga taacacggtc
      901 aatgccctct gcagtgcgag tgctgccgtg agcgatcttg gcgaagtggt tgtcgtgatg
      961 catggaacaa tgagcgatga ctcgtgccat attcatcggg ggaccagggt gagaaagatg
     1021 catacctccc gcaggagcgc ttttgagagt atcggtattc ctcccattgg aagtgtgagc
     1081 tatccttccc tcgaggtgca cctcgaacct gcagccatcc ggagaggaac acaggaacca
     1141 tccctggccg cttctcttga agagagatgc ggactcctcc atttttatcc cggaatgccc
     1201 ccggaagcct tcacttcctt ttccgattac aaagggctca ttattgcagg aaccggtctt
     1261 ggtcacgtca gcaccttact catcccgact ataaaggaac tggtcgatga tggtactatg
     1321 gtggttatga catcgcaatg cctgcacggg agggtatgtg acagggtgta tgataccggc
     1381 cgtgatctga taaaagcagg agccattgag ggaggagata tcctccctga ggttgccctt
     1441 gtaaaacaga tgtgggtcct tggcaacgaa aaggatcctg aacgtgctgc ggtaatgatg
     1501 aagtcctgcc tgaaaggtga gtgcgggggg agatcccttt gatcgattat ggcaaggtcg
     1561 ggctcaaagc tggcattgag atccaccagc agctgaacac cgcgcagaag ctcttctgca
     1621 attgtcccac cacgctcaga acaattgatg agcatgacaa ggaatttttc cgttatttga
     1681 gagcgaccga gagtgaactt ggcgaaattg acgcggcagc aaaggaggag atgaagcagg
     1741 agcggatgtt ctgttacttc ggctatgaca cgacctgcct cgttgagtat gatgaggagc
     1801 cgcctgcacc cttaaaccat gaagcgctcg agctggcctg catcatatcg cgcctttttg
     1861 aaatgacccc tgtcgaacag atccatacca tgcggaaact cgttatcgat ggatcgaaca
     1921 caagcggatt ccagagaacg gcgcttatcg ctgttgacgg gatcctccct aatgggggta
     1981 aaatagagac catctgcctc gaagaagaag ctgcacagag ggttgacgga gataccttct
     2041 cccttgaccg actcggtatt ccgctcgttg agatcacgac ctccccctgt atgaagaccc
     2101 ctgatgaggt ccgcgagatt gccggatatc tgggtatggt gctccgatca accgggaggg
     2161 tcaaaagagg gctcggtact atccgacagg acatcaatat ctcgatagca gaaggagccc
     2221 gcgtggagat caagggagtg caggaactgg acctgatagc agaagtggtc cagcgggagg
     2281 tattaagaca gaaaaatctt gttgacatca ggaatgaact tctttcacgg caagcaacag
     2341 ttatcacaga gcctgttgat gtcacaaaaa tattttacgc ttcctcttca acgatactga
     2401 aaaaagcgca ctgtatcctt gccgtccgac taccgggatt cgccgggctt gtcggaaggg
     2461 agatccagcc aggaagaaga cttgggagtg agttctcgga ttatgcaaag aaatgtgggg
     2521 taggagggat tttccatacc gatgaactcc ccgcatacgg gataacacgt gatgaagtcg
     2581 accgtctttt cgtgcagatc agtgccggat cggatgaatg cattgtgctt gtcgctgcgg
     2641 acgggaagaa agcacattgt gcggcaatgc aggtgattaa gagggctgag accgcattga
     2701 ctggtgtacc cgaagagacc aggaagatgc ttgagggagg aagcacgtca tatatgaggc
     2761 ctctgcctgg agcagcccgc atgtatcccg aaactgatgt ctttcctgtc cggatagagc
     2821 ccacatggta cgagaacctc cccgttccgg aactgcttgt cgaaaaagct gagcggtttg
     2881 tccgtgagct tcatattgat cctgccctcg cttcgcagct tgcatcatca gaccaggtat
     2941 ttctctttga aagggcagtc tcggagggta ttgctccctc ccttgctgca cggaccctgc
     3001 tctcaagcag aaaggaactc gcgagggggg gttcaaatat ctcctgcctg tcagatgaga
     3061 atatcctgat ggtattgaaa gcagttgaag aggggagggc tgcgaaggaa gcagtgccgg
     3121 tgatccttga aaaagtaggg gcgggaacca gtgtttctga ggctattacg gaatgtgccc
     3181 cggccgtttc tcggagcaga cttgaagaaa ttgtccgcat ggtcgtacgg gaaagacgtg
     3241 actttgtcag ggagaaaggc atgggatctc ttgggcctct tatggggatt gtcatggcag
     3301 aggtaagagg ttcagtcgat ggtcgggtgg taagccaggt cctgaaggaa gagatctccc
     3361 gggagctctc ccctaataca cccggtgaca aggtttaaga gaacgcctga cataacaata
     3421 aaggagtttt ccatatgggt aagacaggat cagtacagtg ggtgcaggta aagggtgtga
     3481 aaggtcagat ccggcttgtg cctaaagcgg aaggtgaatt gaagcgggca gggccgaacc
     3541 agcggttcaa agcaggggct tccctcaaga aacttgaggc tgcgaccggt gatgggagag
     3601 gcggccccag aaggggaggc tcccggggtg gacgaggagg aagaggagct cgcggagggc
     3661 gttcggaaca gggacccgcc gcaaatccta tgatccgcag gccgatgcgc agggcaaaag
     3721 catcgattat gggtgccaaa cagaaatcgg cccgttaata ttctgatcct tttttttatt
     3781 ttttttttgg aactatccag caatgccgca acactcatca ccggatgagc tgatagagtg
     3841 agcatggatc tgaagactgc cataaaaacc taccagtttg ccgagcgtgc aaaatcagag
     3901 ctgatcatct gctcccagct cttaaacgcc cttgccggat ttccggagca ggaaagaccc
     3961 ggtgcaaaac ggatgctcct cctggtcatc gaatccgttc gttcagaact tgaatttgct
     4021 ttcagggaca cagaaagggc agagttcaaa aaagggatcg gttttttgaa cgaggcaatc
     4081 agcctctctg agagcgatca gtacgggtca gcagtattaa aattatctga agctatcagc
     4141 gcagccacga cagctgcaca gggagcctgg aaggtgctgg cggacaatgg actcctctga
     4201 atatcttgaa ttcctgaaca cccgctgctc tgtccgtgaa tacagcgatg aagatatcag
     4261 tgatgaagag atcacgtacc tcctgaattg cgtgacaacc gccccgagtg cggggaatct
     4321 tgaagcatgg gatatagtgc tggtccggga tgcagggagg aaggaagggt tatccgatgc
     4381 tgcaatgggg cagctgcagg tgttgaacgc ccctgcagta ttcgtcattt gtgcaaacta
     4441 cgtgcgatcc atgtcccggt atggagatcg cggcattctc tacgcggttc aggacgcaac
     4501 catcgcctgc acgtacatga tgcttgcagc tcacgccctc cagatcagaa cctgctgggt
     4561 cggggcattc gatgaggaga ctgtcaggga gatcgtctcc cttcctcctc atattcgtcc
     4621 gatagcgctc ctctgtatcg ggaaagggag gctgccttca cagcttaccg agcggatggc
     4681 ggtaagcgag catgttcata ttgaggagtg gtaccacaat gcctgattat gttgtaacac
     4741 tggagtcggc ctgggtgatc aaggatgtga agtccctcga tgacgcggta gggatagcca
     4801 tcagtgaagc gggcaaaagg ctgaatccct cagccaaata tgttgagatt gaagccggaa
     4861 tgctcgaatg tccattctgc gagggagaac tgaatagtgc acttattgtc gcaaatactg
     4921 cccttgttgg tctgctgctc gagatgaaag tattcagggc tgactcgcag gatcatgcag
     4981 caaagatagc gaaatccgtg gtgggaaagg cgctccgtga tgttcctctc cttgtcaaag
     5041 aagtttgcga gttatgattc atattgtcgg ccatacggca gttgatcaca tctcccgggt
     5101 ctctgccctc cctgcaaaaa atggatcgtc aaccatcctt caccgtaaag ttctctttgg
     5161 gggaggtgca gctaatatcg cgacagggat agcccggctc ggcggtgaag ttacccttgt
     5221 gagcgcagtc ggcagcgact ttcccggaag cgcgtatgag acctggctga aggaaaatgg
     5281 tgtaaataca aaattttata cggtctccgg aaagaacaca ccgactgcgt tcatgttcac
     5341 cgatccccgg ggagatcaga ttacgttctt tgaatgggga gcttctgaaa ttttcaaagt
     5401 ctctgatccg ccagcgtttc ctttcgtaca catggctacc gcagatcccg aatttaatgt
     5461 gaaagtggcg gaaaatgcgg gattctcctc gttcgatccc gggcaggatc ttcaccgata
     5521 ttcggctgaa cagctctatt cgatcatctc aaagatctca ctcctttttg ccaaccagca
     5581 tgaggttgag agcatgtgcc ggataatgga cctctccctt gatgacctta cgaaaatggt
     5641 tcccatggct gtctttacca gcggtgctga cggcagcacg ctttaccagg gggggaagag
     5701 catacatatc cccgccatcc cggtgaggct ggctgacccc accggtgcag gtgatgcata
     5761 ccgggcagga tttctcaccg catataccat aggacttccc cctgttgtct gttgcagaat
     5821 tggcacggtg acagcatcct atgccgtgga agtcgaggga tgccagacca acctcgcgga
     5881 ctggaatgag atgagggtga ggtatgaaca attttttggg agcactgaca tttaatggat
     5941 aaggatctcc gggaacgtta aatccggaag gaatcagagg agactgccat gggatttgtg
     6001 agtggagatg tgaaaattga gcggctttca cgtttctttt ttgtcgcacc gtcctggccg
     6061 gtttcccttg ccatcatcat cattcttggt atcgttatcg atggagcaag tctccggtcc
     6121 ggacagcata tcaggttctt tggatcgctc tgttttaccc tccccgccct ggcaggattt
     6181 ctcatcacta aaccgctggt tcgtcttctt tcaggacaaa tcacactgaa ccgctctgcc
     6241 ctgctctccc ttacctgtac ggtttttctg ataattatct cccttgccgg catggtggta
     6301 agcatcccct tgttcccctt ttcttatgcg attgctctgg gttttatttt tggtatacgt
     6361 ctggtggttc tggccgccat cgcagattat cgtctcatca ggatgaccct cccggcattt
     6421 gtccagagtg gggtggggat cctgatcgga atggttcttt tcactcctcc cttcgggctt
     6481 tttgccacgc tctccaccat tgtttttggt attgggtttg tactcctgat atgggcactc
     6541 gatcgccccc tttatcgagc attccacatc catgggctga atttcctgaa taccttcctt
     6601 gcacacataa ctgatgggtc aaaaaccatg gaggattttt ttcatgagat cggtgaggaa
     6661 gtctatgttc cccaggtaaa cctcctcttc cgaccagaga acgggggagg ggtcctgttt
     6721 accgtgccaa atatacaccc cggccccctg ggagagatcg gcgggggaaa tctccccaac
     6781 tatcttcagt catctttttc agagctcgtc atggtatcac atggtactgc aacgcatgat
     6841 ttcaacctgg ttgccgagga tgagatcgag aaaattatca gagccatacg aaaagctgaa
     6901 tctgctcttg tcttttcaga caaagctagc agatcgcacc gttatcagta tggttccgta
     6961 tcagttctct atcaggtttt caatgatacc ttgctcatca tcagcacacg atccccggaa
     7021 aagactgaag atattgatct cggtgtgggt atggcgatca tggctgaagg gcatcgtgca
     7081 ttccggcatg tagcatttgt ggatgcccac aactgtttta ccggggatat ctcaacagtt
     7141 cagcctggaa cgctctctgc actggaatac cactctgctg ctcttcatgc catagaagac
     7201 ggcatgcgat tagagcaatc ccggctccgg ttcgggtctg ctcaggtgat acctcccttt
     7261 tcgcggaagg aaggatttgg ggatcaggga atcgaggtgc tggtcattga agccggcgga
     7321 cagaaaactg catatgtcct cattgatggc aataatgtcc tggcgggggt gcgtgacaac
     7381 ctccgcacct ctgtactcaa ccttgttgaa gaagcagaaa tcatgacgac tgattcccac
     7441 gtggtgaata ttctttccgg aaaaaatcct gtagggtatc atgtagaccc ggaattgatc
     7501 ctgccctatc ttgatcaggc tgtaaaagag gctataaaca atctctcctg gggtgaggca
     7561 gcagggagta ccacgctctg cgagagggtt cgtgtttttg gatcacaacg gatggtccag
     7621 atggcaagca cggtcaatgc catgatcctg ttcatacccc cgctctctgc tgccgtgctg
     7681 ctgctggcat tcctcctgtc cctgatgctt tatatgatta ttatttgaaa tgggcattgc
     7741 cggatacatc atatcctctc tcctaatcag gttgatctaa taacaacaat gtcaataata
     7801 acaaccgaac aaagaatatt tcgtagcatt gaaacgaaat cccagacaac cgtaaaacac
     7861 agaacaatca ccaaacagtc taccgtggca gtcttaaaaa aacaggacag tctaccaaaa
     7921 acgtgacagt tgacaaaaac ataccatcat gggccggccc cggtacgcaa ctgatgcatg
     7981 agagtatcag acatccatca attagagtat cagcatttgc gaaccgatac cgggtttccg
     8041 ggccatgttg gcttcatcat ttttttgtct ggtctgtata taaatttttc ttaaaagtcc
     8101 tgataattaa ccgttatttt cacggtaaat gtgtttaatg agcgtttgga cacccatgtt
     8161 cgcagtactt gatctacagg aatagccagc tgttaaataa tctcctgttg gt
//
LOCUS       DAOOCO010000074         8207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8237_length_8207_cov_5.329122, whole genome shotgun sequence.
ACCESSION   DAOOCO010000074 DAOOCO010000000
VERSION     DAOOCO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8237_length_8207_cov_5.329122"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK154_05490"
     CDS             complement(<1..267)
                     /locus_tag="PK154_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPW10550.1"
                     /translation="MHTRWKNWVHVTKLDPDKILPAGAIAEIAASGTDALMLSGTLNV
                     TAKNMAALAEEAKASGLPLVVEPADPEAVLWDGVDLVFVPSVLNS"
     gene            complement(277..3114)
                     /locus_tag="PK154_05495"
     CDS             complement(277..3114)
                     /locus_tag="PK154_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HPW10551.1"
                     /translation="MHLIVAEKNISAHRIASILAGKTKVATDKDGGVPVYRFGDTAVI
                     GLRGHVVEIDFEEGYSNWRSTERTPRSLIDARTIKVPTEKKIVQVLQKLAKKSSLITI
                     ATDFDTEGELIGKEAYELIRDVSREVPVRRARFSAITPDEIRQAFSGTSELDFDLASA
                     GEARQVIDLMWGASLTRFISLAARRGGSNILSVGRVQTPTLAMIVDREKEIEEFVSQQ
                     YWQLSLLTEKDGEEIEARHATPRFCDQTEAVKARDRTHPPLVVTEVKEGAKIERAPVP
                     LDTTGFIVAAARLGFSAAGAMRIAEDLYMNGYISYPRTDNTVYPATLDLGGVLNALSA
                     TEFEEEVAWVTTHRRPVPTRGKKTSTDHPPIHPASAATRHSLGEERWKIYELVVRRFL
                     ATLSPDARWKTMKVNFRAGEVPYTATGGQLVEPGWRKVYPYSTATEYILPPMKVGEQL
                     PIREVTLEEKETQPPARFTQSKLIQKMEELGLGTKSTRHEVIQKLLSRKYIEGTPLRP
                     TLVGRAVTESLERHANTITRPAMTQTLEEHMQQIKEQHRSRDDVVAESRGMLHSVFDQ
                     LEPHEREVGQEIMEQTAEELTLGPCPVCSHDIRIRHLRNQTQFIGCTNYPDCTFNVGL
                     PSAMWGFAVRTDDTCPVHQLHSVRLVRKGSRPWEIGCPLCHQIASNKEALFLMPSMTP
                     AIHEWMIAAHLYTVTDLAGRDPTLLASLLQISREKACDLVREAEEVLGLLRRRSDCRK
                     LIRRMLIPRKGRSPASVIKKLQEAGINDIASLGTADPALLKRVGIADKEADSILHEAK
                     MVENERFLKHAGIPAASMKKYFAAGISSPEDFLSSHPVFISDTTGISLDTVQKHVGLI
                     AAAKGRAAPVRYTKVQVAKGREELTSLPGFSEEDLRRLWKAGIINGLLILESDPKTLG
                     ERTGISPAKLRKHQAVLQERAKKAEPDIIVV"
     gene            complement(3119..4687)
                     /gene="gatB"
                     /locus_tag="PK154_05500"
     CDS             complement(3119..4687)
                     /gene="gatB"
                     /locus_tag="PK154_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPW10552.1"
                     /translation="MTGTADTAGQESRVTDFSDDEVIIGLEIHCQLDTLTKLFCGCST
                     DYRDDGPNTHVCPICLGLPGSLPRVNRKAIEYALRVAKALNCTIVEESEFARKNYFYP
                     DLNKGFQITQYDRPLAVKGYLDIEGDDGEKRVRITRVHVEEDPGRLVHKGGADRAKYT
                     LVDYNRAGIPLIEIVTEPDIRSPKEARKFLNKLRATLEYLGVFDSEKEGSLRVDANIS
                     IQPRGWFDREGKGKADRGRAEVKNISSYKGVEKALTFEVTRQKNALRRGQRLGTETRH
                     FVETRGVTTSSRSKEMEQEYRYFPEPDLRPLRVCAWADEIVLPELPDARKVRFMQNYA
                     FSSTHAATVTGDWKVAEFYDNLPIDLRTQAATATWVADTLLGELNYRDMSISSVPSDH
                     FCHILIDLKEGNITEKSGVEVLRHILDQIKDTGSCESPKNYIEREGLKVISLKGTVEA
                     QSGVTGLLTDDNPIVIAVKEVIAEQTQAVADFAAGKKEAFNFLVGQVMKKTRGRAKPA
                     DVNQVLSGILGKEG"
     gene            complement(4684..5982)
                     /gene="gatA"
                     /locus_tag="PK154_05505"
     CDS             complement(4684..5982)
                     /gene="gatA"
                     /locus_tag="PK154_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPW10553.1"
                     /translation="MATLTFPQDDRYNAFITMLDKAETGPGKLSGIGIAIKDNISTRG
                     IQTTCASRILEGYLPPYDAHVVKLVKKAGSGIVGKTNMDEFGMGTTTENSAFGPTKNP
                     ADTSRVPGGSSGGSAAAVASGMVRMALGTDTGGSIRCPAAFCGIVGHKPTYGRVSRYG
                     LIAYANSLEQIGPMARTVSDISLLMEVLTGYDPSDSTSVNCPYAHIPDPDIRGLKIGV
                     PEEYFGEGVNPRVASVVWDGISLLERKGADIITCRMPSMAYALAAYYVTCTSEASSNL
                     ARFDGVRYGPPADTRQTWHESFQERRRDGFGTEVKRRIMLGTFALSSGYYGKYYAKAQ
                     AARQQVRNDFLRLFREVDVIAGPTMPTVAFRIGEKTDPLSMYLSDILTVPANLAGIPA
                     ISVPCGTSEGLPVGLQIMGPHFRDERVIDVAYAFEQGVSR"
     gene            complement(5983..6252)
                     /gene="gatC"
                     /locus_tag="PK154_05510"
     CDS             complement(5983..6252)
                     /gene="gatC"
                     /locus_tag="PK154_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPW10554.1"
                     /translation="MVSEKDVAHIAELADIGIEKEELAEFTGRFNEILDYFDILDQVE
                     QATRGGTDLVNIFRDDIVEDSLPADEVLANTREKEDGFFRAPRVM"
     gene            complement(6242..7246)
                     /locus_tag="PK154_05515"
     CDS             complement(6242..7246)
                     /locus_tag="PK154_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPW10555.1"
                     /translation="MELKGWVELDGILLSSRELEILLHIEPGSVNRFGGEFFLSWDGC
                     MARDHLGIIPGPCPAGTIVCEGKKTGNVSPNPRVLSLDEAIVTAVRLRSTEGVCAFSG
                     GVDSSLVAFLAQRPCIAIGLAGSHDLARASLAADAMHLDCTCVVVTHDDIREGLHAVI
                     GVIPEPTPVNTAIAITQYLVTRSASELGHERVLTGQGADELFGGYARYRTGTDPAAQL
                     ARDFAGLAAQGLRDQSVAALNGTYLSQPFLDVRVVRAAQSLPAGGMVRDGVGKWPLRE
                     VAARHIPREIAWYRKKAMQYGTGIAKVLQGFARDKGYKRSLQRYLDQIRTNGEARNGI
                     "
     gene            7335..>8207
                     /locus_tag="PK154_05520"
     CDS             7335..>8207
                     /locus_tag="PK154_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPW10556.1"
                     /translation="MGQTVAVDLGATRTRVALIGEEGQIGERREGRTPAKESDPSRLA
                     GFLSGLIGEVRAGHREEPLSGIGLSVAGPVDITRGVLVNPPNMAFRDVPLREELSCLF
                     QCPVRMINDCHAGVLGEMHYGKGQGKQDVVYVTISTGIGGGVVSGGRLLLGRKGNAAE
                     IGHFLVDNTYDLPCSCGHRGHWEGYASGRFLPWFFAEWCRFHAKPHWGPDSAEEIFFS
                     ARQGDDDVLRFVRELSRINARGISDVIVAYDPEIIILDGAVMQSNADLLFPSMREFID
                     RFLPPPEIVLSDLGG"
BASE COUNT         1489 a         2439 c         2475 g         1804 t
ORIGIN      
        1 tgagttcagg accgagggga caaagacgag gtccacgcca tcccagagga ctgcttccgg
       61 atccgcgggc tccacgacaa gcggcagtcc tgaagccttt gcctcctccg caagcgcggc
      121 catgttcttt gcggtgacat tcagggttcc cgagagcatc agcgcatccg tcccgctcgc
      181 agcaatctct gcaatcgctc cggcagggag tatcttgtca gggtcaagct tggtcacatg
      241 gacccagttc ttccatcggg tatgcatcat ctgcacctat accactataa tatcgggctc
      301 cgcctttttt gccctttcct gcagtactgc ctggtgtttg cggagcttcg caggggagat
      361 gccggtccgt tcaccgagcg tcttcggatc tgattcgagg atgaggaggc cgttgatgat
      421 ccccgctttc cagagccggc gcagatcctc ttccgaaaaa cccgggagcg aagtgagttc
      481 ctctctccct ttcgccacct ggaccttggt gtatcgtacc ggtgccgccc ttcctttcgc
      541 cgcggcgatg aggccgacat gtttctggac ggtatccagg ctgatcccgg tggtgtcaga
      601 gatgaagacc gggtgggagg agaggaaatc ctcaggggag ctgatgccgg cagcaaaata
      661 ttttttcatg ctcgctgcgg ggataccagc atgtttcagg aaccgctcgt tctcgaccat
      721 ctttgcctca tggaggatcg aatctgcctc tttgtcagca atcccgaccc tcttcaggag
      781 ggccgggtca gctgttccga gcgaggcgat gtcgttgatc cctgcctcct ggagtttctt
      841 gatcacactg gccgggctcc ttcctttccg gggaatgagc atcctccgga tgagcttccg
      901 gcagtccgat cgccgcctga gcagtccaag gacctcctct gcctcgcgga ccagatcgca
      961 ggctttctcc ctactgatct ggagaagtga ggcgagcagt gtgggatcac gaccggcaag
     1021 gtcggtcacg gtatagaggt gtgcggcgat catccattca tggatcgcgg gggtcatgga
     1081 tggcatgagg aagagcgcct ccttgttcga tgcaatctgg tggcagaggg ggcagccgat
     1141 ctcccacggc ctgctcccct tcctgacgag ccggacagaa tggagctggt ggaccgggca
     1201 ggtatcatcg gtccggacgg caaatcccca catggcggaa gggagaccga cattgaaggt
     1261 gcagtccggg taattggtgc acccgatgaa ctgggtctgg ttccgcaggt ggcgtatcct
     1321 gatgtcatgg gagcagaccg ggcaggggcc gagggtcagc tcctccgccg tctgctccat
     1381 gatctcctgg ccgacctccc gttcgtgggg ttcgagctga tcaaagaccg agtggagcat
     1441 cccccgggat tcggccacga catcgtcccg gctccggtgc tgctccttga tctgctgcat
     1501 atgctcctca agcgtctggg tcatggcagg ccgggtgatg gtgttggcat gccgctccag
     1561 ggactcggtg actgccctgc cgaccagcgt ggggcggaga ggagttcctt cgatatactt
     1621 gcgggagagg agtttctgga tcacttcgtg ccgggtgctt ttggtcccga gtccaagctc
     1681 ctccatcttc tggatcagct tgctctgggt gaaccgtgca ggcggctgtg tctccttctc
     1741 ttcgagggtt acctcccgga tggggagctg ctcgccaacc ttcatggggg ggagtatata
     1801 ctctgtcgcg gtactgtagg ggtagacctt ccgccagccg ggctcgacaa gctgtcctcc
     1861 ggtagcggtg tagggcacct ctccagccct gaagttcacc ttcatcgtct tccagcgtgc
     1921 gtcgggagag agagtggcga ggaaccgccg gaccacgagt tcgtagatct tccaccgctc
     1981 ttccccaagg gagtgacggg ttgcggccga ggcggggtgg atgggcgggt ggtcggtact
     2041 tgtcttcttc ccacgggtcg gtacgggtct ccggtgggtg gtgacccagg ccacctcctc
     2101 ttcaaactcg gtcgcggaga gtgcattcag cactcccccg aggtcaaggg tagcgggata
     2161 gacggtattg tccgtccggg gataggagat gtacccgttc atgtacaggt cctctgcaat
     2221 ccgcattgca ccggcagccg agaatccaag acgggcggca gccacgatga accccgtggt
     2281 gtcgagcggg accggggccc gttcgatctt ggccccttcc tttacttcgg tcactacgag
     2341 aggaggatga gtgcgatcgc gtgcctttac cgcctctgtc tggtcgcaga accggggggt
     2401 ggcatggcgt gcctcgatct cctcgccatc cttctcggtc aggagcgaga gctgccagta
     2461 ctgctgggag acgaactcct cgatctcctt ctcccggtcc actatcatgg cgagggtggg
     2521 ggtctggacc ctgccgacgg aaaggatgtt gctgcccccc ctccgggcgg caaggctgat
     2581 gaaccgggtg agggaagccc cccacataag gtcgatcacc tgccgggctt cccctgcgga
     2641 ggcgaggtca aaatcgagtt ccgaggttcc gctgaatgcc tgcctgatct catcgggggt
     2701 gatcgcactg aacctggccc gccggacagg aacctccctg ctcacatcgc ggatcagctc
     2761 gtacgcctcc ttccctatca gctccccttc ggtatcaaaa tcagtcgcta tcgtgatgag
     2821 gctggacttc tttgccagtt tctggaggac ctggacgatc ttcttctcgg tcgggacctt
     2881 gatcgtccgg gcgtcgatca gggaccgggg ggtccgttcg gtgctccgcc agttggagta
     2941 cccctcttca aaatcgatct cgaccacgtg gcctctgagc ccgatgaccg cagtgtcgcc
     3001 gaaccggtag actgggaccc ccccgtcctt gtcggtggcc acttttgttt tcccggcaag
     3061 gatggaggcg atgcggtgag ccgagatatt cttctcggca acgatcaggt gcatcgcttc
     3121 agccctcttt tccgaggatt ccggagagca cctggttcac gtcagcaggt ttggcccggc
     3181 ccctggtctt tttcatgacc tgcccgacca ggaaattgaa tgcttctttc tttccggccg
     3241 caaaatcggc gactgcctgg gtctgctcgg caatcacttc tttaactgct attacaatgg
     3301 gattgtcatc agttagtaaa cccgtaacgc cactttgtgc ttctaccgtt ccttttaacg
     3361 aaattacttt caaaccttcc ctttcaatat aatttttagg tgattcacaa ctccctgtgt
     3421 cctttatctg atcaaggata tgtcgtaata cttcaacccc acttttttca gtaatgtttc
     3481 cttcttttaa atctatcagg atatggcaga agtgatcgga tggcacactt gagatactca
     3541 tatctcgata attcagctcg ccaagaagag tatcggcgac ccaggtcgca gtagctgctt
     3601 gtgttcttaa atcaattggg agattgtcat agaattcagc aactttccaa tcacctgtta
     3661 ccgtagcagc atgagtggag gaaaaggcat aattttgcat aaaacgcact ttccgggcat
     3721 ccgggagttc cgggaggacg atctcgtcag cccaggcaca gacccgcagg gggcggaggt
     3781 ccggctccgg gaagtaccgg tactcctgct ccatctcctt gctccgcgag gaggtcgtca
     3841 ctccccgtgt ctcgacaaaa tgccgggtct cggtcccgag ccgctgtccc cgccggagcg
     3901 cattcttctg gcgggtcacc tcaaaggtga gcgccttttc caccccctta taggaggaga
     3961 tgttcttgac ctctgccctg ccccggtcgg cttttccctt gccctcccgg tcaaaccagc
     4021 cccgtggctg gatggagata tttgcatcga cacggagcga cccctccttc tcgctgtcga
     4081 agacaccgag gtactcgagg gttgccctga gcttgttcag gaatttccgg gcttctttgg
     4141 gcgaccggat gtccggttcg gtcacgatct cgatcagggg aatcccggcc cggttataat
     4201 cgacgagcgt gtacttcgcc cggtcggcac cccccttatg cacgagcctt cccgggtcct
     4261 cctcgacgtg aacccgggtg atccggaccc gtttctcccc gtcatcgccc tcgatatcga
     4321 ggtagccctt cacggcaagg ggacggtcgt actgggtgat ctggaatccc ttgttcaggt
     4381 ccgggtagaa gtagttcttc cttgcaaact ccgactcctc aacgatggtg cagttcagcg
     4441 cctttgccac tctgagggca tattcgatcg ccttccggtt cacccgcggg agcgagcccg
     4501 ggaggccgag acagatcggg cagacatggg tgttcgggcc atcgtcgcgg taatcggtgg
     4561 agcagccgca gaagagcttc gtaagggtat cgagctggca gtgaatctcg aggccgatga
     4621 tcacctcatc atcggagaag tccgtcaccc tgctctcctg tcctgcagtg tctgccgttc
     4681 ctgtcatcgt gacaccccct gctcaaaggc ataggcgaca tcgatcaccc gctcgtcccg
     4741 gaaatgcggc cccatgatct ggagccccac cgggagacct tccgaggtgc cgcacgggac
     4801 cgagatggcc ggtatcccag cgaggttggc cgggacggtc aggatgtcag agaggtacat
     4861 cgagagggga tcggtcttct cgccgatccg gaacgcgacg gtgggcatgg ttggccctgc
     4921 gatcacatcg acttccctga agaggcggag gaagtcgttc cggacctgtt ggcgggctgc
     4981 ctgggccttt gcatagtatt ttccgtagta gccggaggag agcgcaaacg tcccgagcat
     5041 gatccttctc ttcacctcgg ttccaaaccc gtccctcctc cgctcctgga aggattcgtg
     5101 ccaggtctgt ctggtatcgg ccgggggccc gtacctgaca ccgtcaaagc gggcgaggtt
     5161 ggagctggct tcgctggtgc aggtgacgta ataggctgcc agggcgtagg ccatgctcgg
     5221 catccggcag gtgatgatat cggcaccctt ccgttccagg agtgatatcc catcccagac
     5281 taccgaggca accctcggat tgaccccctc gccaaagtac tcttccggga ccccgatctt
     5341 caggccgcgg atgtcgggat cagggatatg ggcataggga cagttgacac tggtggagtc
     5401 gctggggtca tacccggtca agacctccat gaggagggag atatcggaga cggtcctggc
     5461 catagggccg atctgctcaa gcgagttggc ataggcgatt aggccgtacc gggaaaccct
     5521 gccgtaggtg ggcttgtgcc caacgatacc gcagaatgcc gcgggacagc ggatcgatcc
     5581 gccggtatcc gtaccgagtg ccatccggac catcccggat gcaacggcag cagcacttcc
     5641 cccagaggat ccccccggca cccgggaggt atcggcagga ttttttgtgg gcccgaatgc
     5701 actgttctcg gtcgtggtgc ccatcccgaa ctcgtccatg ttcgtcttgc cgacaattcc
     5761 tgaaccggcc ttttttacga gtttgaccac gtgggcatcg tagggcggga ggtatccttc
     5821 caggatgcgg gaggcgcagg tagtctggat gccccgggtg gagatattgt ccttgatcgc
     5881 gatgccgatc ccggaaagtt tccccggacc ggtctcggcc ttgtcaagca tggtgataaa
     5941 ggcgttgtac cggtcgtcct gcgggaatgt gagggtcgcc actcacatca ccctcggcgc
     6001 cctgaaaaat ccgtcttcct tctcgcgggt attggcaagg acttcatcgg cagggaggga
     6061 atcttccaca atatcgtccc ggaagatgtt cacaaggtcg gtcccgcccc gcgttgcctg
     6121 ctccacctgg tcgaggatat caaaatagtc aaggatctcg ttgaaccggc ccgtgaattc
     6181 tgccagttcc tccttctcga taccgatgtc ggcaagctct gcgatatgtg ctacatcctt
     6241 ctcagatacc attccgtgcc tccccgttcg ttctgatctg atctaagtac cgttgtaaag
     6301 accttttata acccttgtcc cgggcaaacc cctgcaggac cttggcaata ccggttccgt
     6361 actgcatggc tttctttctg taccacgcga tttcccgcgg gatgtggcgg gctgcaacct
     6421 cgcgaagcgg ccattttccg accccgtccc ggaccatccc gccagccggg agggactgtg
     6481 cagcgcgcac cactctgaca tcaaggaacg gctgagagag gtaggtgcca ttgagcgcgg
     6541 ccaccgactg gtcgcggagt ccctgggcgg caagcccggc aaagtcgcgg gcgagctggg
     6601 cagcgggatc ggtcccggtc cggtagcggg catatccccc gaagagctca tcggcaccct
     6661 ggccggtcaa cacccgttca tgcccgagtt cgcttgcaga acgggtgacc aggtactggg
     6721 tgatggcaat tgcggtgttt accggggtcg gttcagggat caccccgatc accgcatgga
     6781 gcccttcccg gatatcatcg tgggtgacca caacgcaggt gcagtccagg tgcatcgcgt
     6841 ctgctgccag cgatgcccgg gcaagatcat gcgacccggc aagtccgatc gcgatgcaag
     6901 ggcgttgtgc aaggaatgcg acaagggatg aatccacccc tcccgaaaaa gcacagaccc
     6961 cctcagtgct ccggagtctc actgcggtga caatcgcttc atcaagggac aatacccggg
     7021 gattgggaga tacgttccct gtcttctttc cctcgcagac gatcgtcccg gcagggcagg
     7081 gtccggggat gatcccgaga tggtcacggg ccatgcagcc atcccacgag agaaaaaatt
     7141 cgccgccgaa tcggttaaca ctccccggtt cgatgtggag caggatctcc agttcccggg
     7201 atgagagcag tatgccgtcc agttccaccc agcccttcag ctccatctgt tcatagctct
     7261 gccctccgcc ccttcatact ctttgtggat gggccgcgaa ccttatgccc cgggcgggca
     7321 aacatcggaa catcatgggg cagacagtgg cagtggacct gggggcgaca cgtacccggg
     7381 tggcgctcat aggggaggaa gggcagattg gggagcggag ggagggacgg accccagcga
     7441 aagagagcga tccctcacgg cttgccgggt tcctatcagg cttgatcgga gaggtcaggg
     7501 ctgggcaccg ggaggaaccc ctctccggga tcgggctctc tgttgccggg ccggttgata
     7561 tcacccgggg ggtgctcgtg aacccgccga acatggcctt cagggatgtt cctcttcgtg
     7621 aggagctctc ttgcctattt caatgccctg tcaggatgat caacgactgc catgccggag
     7681 tgctggggga gatgcattac gggaagggac agggaaaaca ggacgttgtc tacgtgacga
     7741 tctcgaccgg gatcggggga ggagtggtgt cgggggggcg attgctcctc ggcaggaagg
     7801 ggaatgccgc ggagatcggc cacttccttg tcgacaacac ctatgatctc ccctgcagct
     7861 gcggccaccg ggggcactgg gaggggtatg cctccgggag gttcctcccc tggttctttg
     7921 cggaatggtg ccggttccat gcaaagccgc actgggggcc tgattcggca gaggagatct
     7981 ttttctcggc ccggcagggg gatgatgacg tactccgttt tgtccgggag ctctcccgga
     8041 tcaatgccag gggcatctct gatgtcattg tcgcgtacga cccggagatc atcatccttg
     8101 atggggctgt catgcaatcc aatgccgatc tcctcttccc gtctatgcgg gagttcatcg
     8161 accggttcct tcccccccct gagatcgtcc tgtcagatct cggggga
//
LOCUS       DAOOCO010000075         8074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8400_length_8074_cov_4.527996, whole genome shotgun sequence.
ACCESSION   DAOOCO010000075 DAOOCO010000000
VERSION     DAOOCO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8400_length_8074_cov_4.527996"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK154_05525"
     CDS             <1..419
                     /locus_tag="PK154_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10557.1"
                     /translation="YDGDAAQSGAIGNSQSTYLQTTVTGPGTLTFWWKVSSQTRDPLT
                     FSIDGMSRASVKGEVAWQQKTYSITEGTHVLKWEYVKDRAYSAGSDAGWVDKVTFSSP
                     SSPLPKDKIRPPHTALPLQIIRSFVSWFPRPGQFPA"
     gene            complement(667..1866)
                     /gene="ilvA"
                     /locus_tag="PK154_05530"
     CDS             complement(667..1866)
                     /gene="ilvA"
                     /locus_tag="PK154_05530"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HPW10558.1"
                     /translation="MVSIASIREAAALLSGKIIRTPLVYSPTFSERTGAGVYLKLETM
                     QKAGSFKVRGATNRILSRKDSIGPMGVIAASAGNHAQGVAVAAGIAGIPATIVMPVWV
                     SPGKQEAAAGYGARVLLKGNSLEESLAYAQDLAETSAMTFIHPFDDTAIIAGQGTIGL
                     EILEDLPDVETVVVPVGGGGLIAGIATAVKSELRDVRVIGVQVSACPSAVAARKAGGP
                     LTVASHPTIADGIRVTRTGEVTWPVIRDLVDEIVVVDDNQVIAAITALLERKKVLAEG
                     AGAAPLAAVLSGDITLHPGEQVVAVISGGNLDSFLLERVIRKGLFDAGKMLRCTVDIE
                     EEVIALPALLSLIAGEGAIISRIEQERGSPDLPLHLNRVTLEMELRGPEHRYRVLQVL
                     RDHGYRV"
     gene            2251..2592
                     /locus_tag="PK154_05535"
     CDS             2251..2592
                     /locus_tag="PK154_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10559.1"
                     /translation="MMLTRYQKIMLAAFIFLMAADFAISSYGVLFLPGFTEANALFAR
                     FMAYPLTFIAVVGVTKLLVIAGLIAATIWFNQREREDDPWHGGTLLCSTAAVGMGVMM
                     LVLIIANLLLI"
     gene            2786..2911
                     /locus_tag="PK154_05540"
     CDS             2786..2911
                     /locus_tag="PK154_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10560.1"
                     /translation="MTGDIRRFAGTVKALVRARLPGSPDGGAAVLNRTMTFGLFR"
     gene            2996..4342
                     /locus_tag="PK154_05545"
     CDS             2996..4342
                     /locus_tag="PK154_05545"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HPW10561.1"
                     /translation="MLAGGSGTRLFPLSRGHFPKQFLPIFDGHSLYQLTLRRALIFSS
                     PEEIVVVTNPDQEFIARDQAGMIGCGCRILSEPEGKNTLPAVYYGLREIEQSSGPAKV
                     AVLPSDHMVHAGPVYADALRKAEELADKWLVLFGVKGTEPHTGYGYIQPGPAIGGAYS
                     VSSFTEKPDRVTAERYLQDGYLWNSGMFLFSTRLFFEECGIHAPDIARAFTRPLEEAY
                     REVPSVSIDYGLMEKTSRAAVVPLETGWNDLGSFDAFYSVLQKNGEENAVRGEYIGLD
                     STGNLVIGDHLVITIGVHDTAIIDSGDVTLIAPRQRAHEAKEAVALLRQKGDPRADSH
                     LTEYRPWGSYTRLEDGPSYRIKRITVPQGRRLSLQMHHHRSEHWVVVHGTAKVHIDGQ
                     ERLLRKGESTFVPIGAVHRLENPGLVPLEIIEVQIGEYIGEDDIVRLQDDYQRHES"
     gene            complement(4784..5365)
                     /gene="rfbC"
                     /locus_tag="PK154_05550"
     CDS             complement(4784..5365)
                     /gene="rfbC"
                     /locus_tag="PK154_05550"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPW10562.1"
                     /translation="MKKFERIGTPLEGVVIIQPHIFSDDRGFFLESYNAEEFTELGIG
                     EAFVQDNHSCSRKGVIRGLHYQSRYPQGKLVRVLRGSIHDVIVDIRKGSPTYGMNYGI
                     VLSDDTYRMAYIPPGFAHGFLALEDRTEVHYKTTDFYHPEYDAGIRWNDPTLGIQWPL
                     VENGIGHPLVSPKDALLPFFTEIVSPFQYSSEE"
     gene            complement(5379..6254)
                     /gene="rfbA"
                     /locus_tag="PK154_05555"
     CDS             complement(5379..6254)
                     /gene="rfbA"
                     /locus_tag="PK154_05555"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPW10563.1"
                     /translation="MKGIILAGGFGTRLYPVTKSISKHLLPVYDKPLIYYPLSVLMLA
                     GIREILIISTPRDIPHYKELLGDGTQLGLTFSYAIQERPGGLAEAFIVGEQFIGKDSV
                     ALVLGDNIFYGQHFSDYLKRAGNLKKGAIIFGYYVKDPKAYGVVELDAEGNPVSIVEK
                     PQHPVSNYAVPGLYFYDNDVISIAKQLRPSGRGELEITDINREYLNRGTLEVLLFGRG
                     MAWLDAGTHDSLLDAANFIEAIQKRQGLYVACIEEIAFTMGYIDAHQLHGLSQSLKNT
                     EYGAYLEQIATGSDF"
     gene            6584..6736
                     /locus_tag="PK154_05560"
     CDS             6584..6736
                     /locus_tag="PK154_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10564.1"
                     /translation="MQLEKDDIKLIEDKLALIESNIRAIREYLDERTMEIQELSDDIM
                     KIVRKG"
     gene            7219..7291
                     /locus_tag="PK154_05565"
     tRNA            7219..7291
                     /locus_tag="PK154_05565"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7253..7255,aa:Gln,seq:ttg)
     gene            complement(7551..>8074)
                     /locus_tag="PK154_05570"
     CDS             complement(7551..>8074)
                     /locus_tag="PK154_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10565.1"
                     /translation="GQEASTAWALLTLERSVIIPVLEVPVDMKPGSCPNSYNVGQKGV
                     LPAAILSTDEFDVSDIDPATIRLVACDEEDPAAGVAPLRWNYEKVAQPAEDANCDCEA
                     CDEYNCWESAYDGDVGDEYMDLTLKFDSVAVSAYLGDVDPGCMKIKLVGTLKEEEGGT
                     AFEGCDTLRLLVK"
BASE COUNT         1796 a         2212 c         2163 g         1903 t
ORIGIN      
        1 actatgatgg ggatgccgcc cagagcgggg cgattgggaa cagccagtcg acctacctgc
       61 agaccacagt caccggcccc gggactctga cattctggtg gaaggtctcc tctcagacca
      121 gggatcccct caccttctct atcgatggaa tgtcgagggc aagtgtaaaa ggagaggtcg
      181 cctggcaaca aaagacctac agcatcaccg aagggacgca tgtcctgaaa tgggagtatg
      241 tcaaggaccg ggcatattcc gcaggatccg atgcaggttg ggtggataag gtgacatttt
      301 catcaccctc ctcccctctt cccaaggata agataaggcc tccacatact gctctgccgc
      361 ttcagatcat caggtccttt gtctcgtggt tccccaggcc aggacagttc cctgcataaa
      421 aatcctcact cgtcaggtca acgtaacgta ctgttttatc tcaattgttt cagcaaagcg
      481 attgtcgttc gcttgtagtt tctgtccgga cgtacctatg gtaaaaatgt gagcgagaat
      541 cgcaaggata actgtcatca gcagctgggg gacgatgccc gctcgcaccc tttcctgatc
      601 gccctcgttc tttaacgcag gacttccctt cgaccagatc cctgggaaat ctccggcagt
      661 acacactcaa acccggtacc catgatctct gagcacctgg agcacccggt accggtgctc
      721 gggacctcgc aactccattt ccagcgtcac cctgttcagg tgaaggggca ggtcgggaga
      781 tccccgctcc tgctcgatcc gggagatgat agcaccctcc cctgcaataa gagagaggag
      841 tgcaggaagt gctatcacct cctcttctat atcgaccgtg caccggagca tttttccggc
      901 atcaaagagc cctttccgga tgacccgctc gaggagaaac gagtcaaggt ttccccccga
      961 gatgacggca accacctgtt ctcccgggtg gagggtgata tccccggaaa gaaccgctgc
     1021 aaggggtgct gcccccgctc cttcagcgag taccttcttt ctttcgagca gtgccgtgat
     1081 ggcggcgatg acctgattgt catcgaccac cacgatttca tcgacaaggt cgcggatgac
     1141 cggccaggtt acctccccgg tccgcgtcac ccgtatccca tcagcgatcg ttggatggga
     1201 cgccacggtc aacggtcccc cggcttttcg tgcggcaaca gccgaggggc atgccgagac
     1261 ctgcacccct atcaccctca cgtcacggag ttcagacttc actgcggtgg ctatgcctgc
     1321 aatcagcccc cctcctccga cagggaccac aaccgtctcc acatccggga ggtcctcaag
     1381 gatttcaaga ccgatcgtac cctgccctgc aataatagcc gtatcatcga aggggtggat
     1441 gaacgtcatc gcactggttt ctgcgagatc ctgtgcatag gcaaggctct cttcgaggct
     1501 gttgcccttg agaagtaccc tggcaccata ccctgccgcc gcttcctgct ttcccggcga
     1561 gacccagaca ggcatgacga tggttgccgg gattccggct atacctgcgg cgacagcaac
     1621 tccctgcgca tggttgcccg cggaagccgc gatcacaccc atgggaccga tcgaatcttt
     1681 ccgggacagg atccggtttg tggcgcctct gaccttgaag gatcctgcct tctgcatcgt
     1741 ctcgagcttg agatacacgc cggcacctgt cctctctgaa aatgtcgggg agtagaccag
     1801 aggagtccgg atgatcttcc ctgaaagcag tgcagctgct tctctgatcg atgcgatgga
     1861 gaccatgggt agggatctgg agtctggcat catatgaatt gtggaagggt catgggaaaa
     1921 aggtgacccg gttaaatcac ggtatgaggg agcacgtgga gaacggagcc cgatgggtca
     1981 tcggactaat cccaatggta taaccgagtg gaggggcagt tcctatggta ccacgaagaa
     2041 acaattaacc gaaaaaggga acatggccca atcctgtcaa aatggtttct tcgacagtgg
     2101 cgtgatcccg gaaaaatacg aaccacggat tgtccctatg gggatggaaa acgacaaccc
     2161 agggattctt acccagggat gcatgccctt ttcacgtcgt cagtttgcct ctatcagaaa
     2221 ctcattaaat gtgggattcg atggtaatgt atgatgctca cccgctacca gaagatcatg
     2281 cttgcggcat tcatcttcct gatggcggct gactttgcca tctcgtcata cggggtcctc
     2341 ttcctccccg gtttcacgga ggcaaacgcc ctctttgccc ggttcatggc ctatcccctc
     2401 acctttattg ccgtcgtggg agtgacgaag ctcctggtga tcgccgggct catcgccgca
     2461 acgatctggt tcaaccagag ggagcgggag gatgatccct ggcatggcgg cacactcctt
     2521 tgttccacgg ccgcggtcgg gatgggtgtg atgatgctcg tgctcatcat cgccaatctc
     2581 ctcctcatct gatcggacga gtaccgatct ggctggttcc ggggtgccgg atgccacagt
     2641 gaggagtttt attatgttcc gctatcgtat tgctgagtat cgggtgatat acgcacgaat
     2701 ccctgattct gttcttattt tacgcattgg tcacaggaga gcagtctccc ggtaaatagt
     2761 gccggtcttt ttgaaaatcc tcgcgatgac cggggatatc cgaaggtttg ccggaaccgt
     2821 caaggccctg gttcgtgccc gtctgccggg ttcccctgat gggggagcgg cagtgctgaa
     2881 tagaacgatg acctttggtt tgttccgctg aaacccgctg gtgaaataac cgatataagg
     2941 ggggagatgc catattctcc actatcatgg aggaggattc atcatacaca ctctgatcct
     3001 tgccggcgga agcggcacac ggctgtttcc cctcagccgg gggcacttcc ccaagcagtt
     3061 cctcccgatc tttgacggcc actccctcta ccagctcacc ctccggcgag ccctgatctt
     3121 ctcctcaccg gaggagattg ttgttgtcac caatcccgac caggagttca tcgcacggga
     3181 ccaggccggg atgatcggat gcggctgccg gattctctct gagccggaag gaaagaatac
     3241 ccttcctgcg gtctattacg ggctccgcga gattgagcag tcgtccggac cggcgaaggt
     3301 cgcggtcctg ccctccgatc acatggtgca tgcagggccg gtgtacgctg acgccctgcg
     3361 gaaagccgag gagcttgctg acaagtggct ggtcctcttc ggggtgaaag ggaccgagcc
     3421 ccataccggg tatggataca tccagccggg gcctgcaatc gggggggcgt attccgtctc
     3481 ctccttcact gagaagcccg accgggtcac cgccgaacga tacctgcagg acggctacct
     3541 gtggaacagc ggcatgttcc tcttctcaac ccggctcttc ttcgaggaat gtggtattca
     3601 cgcccccgat atcgcccggg ctttcacgag accactggag gaagcatacc gggaggtccc
     3661 ctccgtctcg attgactacg gactgatgga gaagacctcc cgtgccgccg tggttcccct
     3721 tgagaccggg tggaacgatc tcggctcgtt cgatgcattt tactcggtcc ttcagaagaa
     3781 cggggaggag aatgcggtgc ggggcgagta tatcggcctt gattccaccg gaaacctcgt
     3841 tattggtgat cacctcgtga tcacgatcgg cgtccatgac accgcgatca tcgattccgg
     3901 agatgtcacc ctcattgccc cgcggcagag ggcacacgag gcgaaagagg cggtggccct
     3961 cctgcggcag aaaggggatc ccagggctga cagccacctg accgagtacc gcccatgggg
     4021 ctcgtatacc cggcttgagg acgggccctc ttatcgcatc aaacggatca cggtacctca
     4081 agggcggaga ctctccctcc agatgcacca tcaccggagt gagcattggg tcgtggtcca
     4141 cgggaccgca aaggtccata tcgacggaca ggagcgcctc ctccggaaag gagagagcac
     4201 gttcgttccc atcggtgcgg tgcaccggct ggaaaatccc gggcttgtcc ctctcgagat
     4261 catcgaggtg cagatcgggg aatatatcgg cgaggatgat attgtccggc tccaggatga
     4321 ttaccagcgg catgaatcct gataggaccg gatggcaccc tctctttttt gaatggcatc
     4381 tcatgttcga ccgggtaact cctctgcgat caatgatggg atgagcggag catcgtggta
     4441 acagcaggaa cctgatcata agaactaccc tctttcttca gcaggaaccg gatcataaga
     4501 actacctttt ttcttcagca ggagcctgat cataagaact accttctttc ttcagcagga
     4561 gcctgatcca ttggtgaaca taagaactac cttctttctt cagcaggtca ccgcccctct
     4621 ctccccgaaa aaatggtatc ggtggcatac tccggctgga tgcaggctct tcatgaaaaa
     4681 tgggagagga accctggtga tgaggatcca ctgatctcct ctccagggat catcccttgt
     4741 cgaacagaga gcatgtcctg gtgaggaacc ctgtctttcc ctattattct tcagaggagt
     4801 actgaaacgg gctaacgatt tcagtgaaga aggggagcag ggcatccttt ggtgaaacca
     4861 gaggatgtcc gatgccgttt tcaaccaatg gccattgaat tccaagcgtg ggatcattcc
     4921 accgtattcc ggcatcatat tccgggtgat agaaatcggt tgttttgtaa tggacttcgg
     4981 tccggtcttc cagggcgagg aacccgtggg caaatcccgg ggggatgtac gccatccggt
     5041 aagtatcatc agaaaggacg atcccgtagt tcattccata cgtgggtgaa cctttccgga
     5101 tgtcaacgat gacatcatgt atcgacccac gcaacacccg gaccagtttg ccctggggat
     5161 acctgctctg ataatggagg ccgcgtatga cccctttcct tgagcaggag tggttatcct
     5221 ggacaaacgc ttctccaatc ccgagctcgg tgaactcttc tgcattgtat gactcgagga
     5281 agaatcccct gtcatctgaa aaaatgtggg gctgtatgat cacgaccccc tcaagcggtg
     5341 tcccgatgcg ctcaaatttc ttcatgaggt cgcccctctt aaaagtcgct cccggttgcg
     5401 atctgttcca ggtatgctcc gtattcggta tttttcagcg actgtgacaa tccatgcagc
     5461 tggtgagcgt cgatatatcc catcgtgaag gcaatctctt cgatgcatgc cacgtagagt
     5521 ccctgccgtt tctggatcgc ttcgatgaaa ttcgcggcat ccagcaggga atcatgcgtt
     5581 cccgcatcaa gccaggccat ccctcgcccg aagaggagga cctcgagcgt tccacgattc
     5641 agatattccc tgttgatgtc ggtgatctca agttcaccgc gaccggaggg tctgagctgt
     5701 tttgcgatgc tgatcacatc gttgtcatag aaatagaggc ccggcactgc atagttggag
     5761 accgggtgtt gcggtttctc aacaatggat accgggttgc cctctgcgtc cagttccaca
     5821 acgccgtatg ccttcggatc tttcacgtag tatccgaaga taattgcacc ttttttgagg
     5881 tttcctgccc ttttcagata atcggagaaa tgctggccat agaagatatt atccccaagc
     5941 accagggcta ccgaatcctt gccgatgaac tgttctccga caatgaaggc ttctgccagt
     6001 ccccccggcc gctcctggat tgcataggaa aaggtcaggc caagctgtgt tccatccccg
     6061 aggagttctt tgtaatgggg gatatccctc ggggttgaga tgatcagtat ctcccggatg
     6121 cctgcaagca tgagcacgga cagggggtaa tagatgagcg gtttgtcata gacgggcaga
     6181 aggtgtttgg atattgattt ggttaccgga tagagtcgtg taccaaagcc cccggcaaga
     6241 atgatccctt tcatgatgcg ttcctatata gatatgcggc agtcttcact tatactctgc
     6301 ctgtggtggt acacagggga cggttcccgg cagatcggta gtattggtac gatctcaagg
     6361 ttatttcccg gaacgtcgat cacatactat gggcccttat tggtcagtaa tgccatctgc
     6421 ctggtgagga ccagttctcc ctatgaaaag gcttgttgga gtggataatc aacccgataa
     6481 cggtaccctt gaggagggag caatgatgcc gttacgcgta taaagatatc aaaaccaaaa
     6541 gaacttggaa acccgggaat tatcagggag gtgggaaata cccatgcaac tggaaaagga
     6601 tgatatcaaa ctcattgagg ataagctcgc cttgatcgaa tcaaacatca gggctatcag
     6661 ggaatacctg gatgagcgga ctatggagat tcaggaatta tccgatgata ttatgaagat
     6721 cgtccggaag ggatagatca tgcgcatccc gtccctcttc agggatgatg aaagccctcc
     6781 ccctcctctc gcaaatgggg ctcctcattc aaaagaggtc ctgcctctgg cagtctcttc
     6841 cttcatgcag acctgatgga ggtcaaggga ggttcacaag aaaaatcctt cgctcttttc
     6901 ctgttatctc aatgagactc atggaatttt tggtcttcct ctgtaagagc tattctgaag
     6961 gggggggtga ctgacgggag taaggtgggt accaaaaaag acatagctct ttctgatggc
     7021 tgctcttacg tcctttatcg atcacatccg gcatggccat cactgcaccc tggccgtctg
     7081 ttcaggcaat ctcccccatc aaggctctat ggtgtgagtg gtaaaagacc caaaatagat
     7141 agccatgaat gaagggtatc cttatccatt tttatggtgt atctataatg acctcttctg
     7201 ttccaggaag ggtcaggcac ccccgtagtg tagcggtcaa tcatgcagga ctttggatcc
     7261 ggcgacacca gttcgaatct ggtcgggggt attgcttctt cacgcggttt tcagggttga
     7321 acattttttg tcggatcgtg gtttaccgaa cagaaccgtg ttgtcctgcc tcagcgaaag
     7381 ctcacctcca gcccttgatg gatactatcg tgtcatcaat cagtggatat cgaaaatctg
     7441 agatggctca ggggagatca ttgacgttct ctgcttgagg aagattacag gaaggatata
     7501 atttccgggt aaaaaattga gcggatatcc cattctggga tttcccctgg ttatttcaca
     7561 agaagccgga gggtgtcaca tccctcgaat gcagttcctc cctcttcttc tttcagggtg
     7621 ccaacgagtt tgatcttcat gcaaccggga tcgacatcgc caagatacgc agagactgcc
     7681 accgaatcaa acttgagggt cagatccatg tattcgtctc ccacatcgcc gtcataggca
     7741 ctctcccaac agttgtattc gtcacatgct tcacaatcgc agttagcatc ttcagcaggc
     7801 tgtgcgacct tttcatagtt ccagcggagc ggtgcaactc ccgctgccgg gtcttcctca
     7861 tcgcaagcga ccagccggat tgttgctgga tcaatgtcgg acacatcaaa ttcatcagtg
     7921 ctaaggattg ctgcggggag tactcctttc tgaccgacat tgtatgagtt cgggcatgaa
     7981 cccggtttca tatccaccgg gacctcgaga acggggatga taacggaacg ctcaagggtc
     8041 agcaaagccc atgctgttga agcttcctgt cctg
//
LOCUS       DAOOCO010000076         8057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8426_length_8057_cov_4.442139, whole genome shotgun sequence.
ACCESSION   DAOOCO010000076 DAOOCO010000000
VERSION     DAOOCO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_8057_cov_4.442139"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PK154_05575"
     CDS             complement(<1..1482)
                     /locus_tag="PK154_05575"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPW10566.1"
                     /translation="MSPNDTSVMLCAIFIFIAVVAAGCMSTLSPAPSVPAELKKFNST
                     AEIEQYLKDSLAASQQDGYYPPSLATGGMGAGVNAPQESAEKSDSGIPASSRPGSIDY
                     SQTNIQVKGVDEPDIIKNDDRYIYTISGQTLAIIRAYPPEGASVVSETEINDTPKELF
                     VSGDRLVLFCTGTEDSDTRPDTTDPWRNPVPPYYRPYSPVTFTTIYDISDRNHPTMLK
                     EYTIEGDYLDARMIGSLVYQITREQISPYYGDHIVVPALREGSRTVVQPDVWYFDHPE
                     SQYTFTTITSLDASSGKEKDAQTYLLGTGSTLYASEDAIYVSYPRYHPVIYKGQAQEL
                     SPGLAVDASGSVGASSSKIAMIPPDFNTLTEGERESILDGLRSAEQDAIRRQEADQTT
                     TVIHKIGIRDGAITYLAKGEVHGTLDNQFSMDEYKDNLRVATTSTIYTPQYGQYTYNN
                     VYVLDDSMATIGELTHIAEQETIYSTRFIGDRLYMVTFKRIDPF"
     gene            1837..2535
                     /locus_tag="PK154_05580"
     CDS             1837..2535
                     /locus_tag="PK154_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPW10567.1"
                     /translation="MVHLLEDSALRNCEAVFRDRNDAGRRLAAFMRQHRPVTDPLICA
                     IPAGGVPIGLELAKGIPARMFLGVVRKLKIPWNPEAGFGSMTWNGKVYLNQELVRILN
                     LTGEEIRQAVAETGKNIRERIDHFTGGLPIPPIHGRTVIITDDGLASGYTMMAAVNAL
                     NSENPDEIIVAVPTGSAKAVAMISGMVATTICLNLRDSYPFAVADAYQNWYDLDDEEV
                     IRSLSLARRNQLLC"
     gene            2962..5505
                     /locus_tag="PK154_05585"
     CDS             2962..5505
                     /locus_tag="PK154_05585"
                     /inference="COORDINATES: protein motif:HMM:NF041431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HPW10568.1"
                     /translation="MDQKEPGFGTMRFGWQAAWATTMLWIVLLMLLISPAGSVTTIIS
                     PGDQLFIGEEGLDVRAAVAETANQIAWFSPGSDPDTDVPALIIPLGNKQEFYVSPSQF
                     GHATGIWYSWNRTVIGPAFQVQCPALTLRVWNQETGEDVSGLSIAPGTLVNFRIETNM
                     ASLTRRAGYDPASDGYLSIVLVSPTGSTLTSVAGPDGTLLPLKHLDVNSSLWYWVPPQ
                     TPSGWDTAAQSATGSRLYPSGTYIARLDYNVNHLQDNTEGSALFCRPLPVQVALASER
                     VSASSGAAEVVLGNPFTITISGVPGADYYLWVKSTGSMSGNPGDQPPQITPSQEGVLT
                     DPVNGPYAIGSHQISTRQAMTIRDDVPPAPANGTPYYALVTLTGSGVRTVQWQTSSAT
                     DDRPYTFQVERVSPGGVSFDEVSIVVVRGSVSIGTGPPRSFFLGEQVLLAGTNTESDT
                     VYLFMTGPNLPSAGGSLTNPRIPVRTGQPESFTAVSVDPDNTWEYRWFSGNLGIDAGT
                     YTVYAVAGPASRTDLSLWRYSTSSFSFARPFVTAGIPQATVARGDPVEVRGTATGNPD
                     PGVALWIFGINRFLYATEEVNADGSFTYTLNEGETTDLATGQYYLVIQHPGYTDTFDV
                     YPSPNRQLVLSSYPVPGSALFRVGGPGALMSSQAADALVRSLASPFIDDTYVALSFLL
                     VNPQVTIAPPGNSTIGGLINLSGTTNLAPGNQLLVEVTSQAFGPSPKEGGGAFSGVSG
                     IAPIREGSGGQNNWSFSFDTDSFVADTYLVTVSGVTVSNAVATTSFSLSAATPTPTSS
                     TPATPQPTITIPTTDPTPFPTTTASLSWFALAGAVVFILIAFGSAGRNG"
     gene            5828..6718
                     /locus_tag="PK154_05590"
     CDS             5828..6718
                     /locus_tag="PK154_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10569.1"
                     /translation="MTQFDPVFLAIANLGVLIFIIASMLGMGFNLTIPQILAPLKNRK
                     LVIMALVANFVVVPVLALLIVWIIPLSEGLQIGLILVGFAAGAPFLPKLVQLAKGDMA
                     FTAGLMVLLMVISIAFLPIVLPFVLPGVQVSPWEISKSLIVLMLIPLALALFIRARYE
                     EVATGLVHTMTMAANLSLAAMFIGYFVAYFDESFGILGTGGVLAAVLLVIGAVLTGFL
                     LGGTNKEIKKVLALGTGQRNLAAAFAIATSNFAANPDVVLEVMDVAVIGFILLMVIAG
                     ELGRRSARRAGKGLKAAVRD"
     gene            complement(6799..6969)
                     /locus_tag="PK154_05595"
     CDS             complement(6799..6969)
                     /locus_tag="PK154_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HPW10570.1"
                     /translation="MYNRSNYGSPRTTGPREMTKVVCSDCGKDCEVPFKPTEGRPVYC
                     QECLPKHRQPRY"
     gene            7727..>8057
                     /locus_tag="PK154_05600"
     CDS             7727..>8057
                     /locus_tag="PK154_05600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW10571.1"
                     /translation="MTSVAVESWKKVQLETNMGTIVIALDPSMPITAGNFETLVKAGY
                     YNGVIFHRVIDEFMIQGGDPTGTGGGGPGYMIPDEFQPGNRNDRGTVAMANAGPNTGG
                     SQFFINLV"
BASE COUNT         1689 a         2234 c         2254 g         1880 t
ORIGIN      
        1 gaacgggtcg atccgcttga aggtcaccat gtagagccgg tcgccgataa accgcgtcga
       61 gtagatggtc tcctgttcag cgatgtgggt cagttcaccg atcgttgcca ttgagtcatc
      121 caggacatag acgttattgt aagtatattg cccgtactga ggggtgtaga tggtggacgt
      181 ggtagcgacg cggaggttgt ccttgtattc atccatcgag aactgattgt ccagggtacc
      241 gtggacctct ccttttgcaa ggtaggtaat ggcaccgtcc ctgatgccga tcttgtggat
      301 caccgttgtg gtctgatcgg cctcctgccg gcggattgca tcctgttcag cgcttcggag
      361 accatcaagg atcgactccc gctcaccctc ggtaagggtg ttgaagtcgg gcggtatcat
      421 ggcgatcttt gaagacgagg ctcccaccga tccactcgca tcaacggcca ggcctgggga
      481 gagctcctgt gcctgcccct tgtagatcac cgggtgatac cttgggtaac tcacgtagat
      541 ggcatcttct gatgcgtaga gggtgcttcc ggtcccgagc aggtaggtct gggcgtcctt
      601 ctccttcccg ctcgaggcat caagagaggt gatggtcgtg aaggtgtact gggactcggg
      661 atgatcaaag taccagacat cgggctggac cacggtcctt gacccctcgc ggagggccgg
      721 gacaacgatg tgatcgccat aatagggtga gatctgctcc ctggtgatct ggtataccag
      781 cgaaccgatc atccgggcat cgagataatc gccttcgatc gtatactcct tcagcattgt
      841 tggatggttc cggtcactga tatcatagat ggtcgtgaag gtgaccgggg agtaagggcg
      901 gtaataggga ggcacgggat tcctccacgg gtcggtagta tccggccggg tatcggaatc
      961 ttctgttcct gtacaaaaga gaacaagccg gtcaccgctg acaaagagtt cctttggtgt
     1021 gtcattgatc tcagtctcgg agaccactga tgcaccctct ggcgggtagg ctctgatgat
     1081 ggcgagggtc tggccggaga tcgtgtagat ataccggtcg tcatttttta tgatgtcggg
     1141 ttcatcgacc ccttttacct ggatgttggt ctgcgagtaa tcgatgctcc ccggccggga
     1201 ggaggcaggg atgccggagt ctgacttctc tgctgactcc tgcggggcat tcaccccggc
     1261 gcccatgccc ccggtagcaa gagagggggg atagtacccg tcctgctggc ttgctgcaag
     1321 ggagtctttc aggtattgct ctatctcggc agttgagttg aacttcttca gttctgctgg
     1381 aacggaaggg gcgggggaga gcgtgctcat gcatcctgct gcaacgactg cgatgaagat
     1441 gaatatggca cagagcataa cggaggtatc gttcggggac attatctcac gctagcgtat
     1501 tgggagatga tctcttcaat ctggcgttga cttcgaaaat tttcgcaggt atcctggaat
     1561 aaaaacatga aaaaaggtcg tattttagtt tttttcaatt ctgactataa ccagggagga
     1621 ggtggaacaa acagtatgat gatgatgatg ttccactgct attacaaggg acgggggttt
     1681 ccatctcaac gctgatggtg ccgccacggt tacttcgagg acttccgtgt tgaaaaaaat
     1741 gctttctgga atgatgcatc aggcatacag cttcgcacag ggtgggaaga attttaaacc
     1801 ggcagggaat accgaaggct ggaagcacgt ggattcatgg tgcatctcct tgaagattcg
     1861 gcactccgga actgcgaggc agtattccgg gatcggaatg atgccggcag acgccttgct
     1921 gcattcatgc gtcagcaccg tcccgttaca gatcccctga tctgtgccat tccggcgggg
     1981 ggagtcccga tcgggcttga actggccaaa gggattccgg cacggatgtt ccttggcgtg
     2041 gtgagaaagc tgaagatccc ctggaatccc gaggctggtt tcgggtcgat gacatggaac
     2101 gggaaggtct acctgaatca ggagctggtc aggatcctga acctgaccgg ggaggagatc
     2161 cggcaggcag ttgcggagac cgggaagaac atccgtgaac ggatcgacca ttttaccggt
     2221 gggcttccga tccctccaat ccatgggcga acggtaataa tcaccgatga cgggcttgcg
     2281 tcaggataca cgatgatggc ggcagtgaac gcactcaatt ccgagaaccc agatgagatc
     2341 attgttgctg tgcctacggg gtccgcaaaa gcagttgcca tgatctccgg catggttgct
     2401 actaccatat gcctgaacct gcgggactcc tatccctttg cggtcgctga cgcctatcag
     2461 aactggtatg acctcgatga cgaggaggtg atccgctccc tttccctcgc acggagaaat
     2521 cagctcctgt gttgacctat cagggaggac ggcggggcat tatccctcac gttcatcggg
     2581 ttgtaccagc aacctctcag ggtaccactc tgattccatc cgtcaccgcg ttattgcggg
     2641 atctcccggg atggtgctcg cattgctccc agatttgtga atgaagggtg catccggagt
     2701 tgaaatactg gtgctagatc aagaatatcg gaaaagcttc cgcaatcagc tcgcttccca
     2761 ggctgtcatc tccttctcac cttcctggtc ttcatcaagg acattcatct catccgggtc
     2821 cgtaccgcgt agagatgata tggtacattt tgaggttcgc ccatactttt ttcaacagcc
     2881 cctccaaact gtcccctgtc aatggtatcc gggacaggaa caggagtgtt cactgccgga
     2941 tcagcacaga ggagatgggt gatggatcag aaagaaccgg gttttggaac gatgcgattc
     3001 ggctggcagg ctgcatgggc aacaacgatg ctttggatcg ttctgctcat gctgctgatc
     3061 tcgcctgctg gatcagtgac caccattatc agccccggcg atcagttgtt catcggtgaa
     3121 gagggactgg atgtcagggc tgcggttgcg gaaactgcca accagatcgc ctggttctct
     3181 ccaggatcag atccggatac cgatgttccg gcactgatca tccctcttgg gaacaaacag
     3241 gaattctatg tctccccctc gcagttcgga catgcgaccg ggatctggta cagctggaac
     3301 aggacagtga tcggcccggc gttccaggtc cagtgccctg ccctgaccct gcgggtgtgg
     3361 aatcaggaga cgggcgagga tgtctccggg ctctctatcg ccccgggaac actggtaaat
     3421 ttccggatcg agaccaacat ggcgtcgctg acgcggaggg caggatacga tcccgcttca
     3481 gacggatatc tctcgatcgt cctggtctcc cccacgggat cgacgctgac ctcagtcgcc
     3541 ggacctgatg gcaccttgct tcccctaaag caccttgatg tcaattcatc gctctggtac
     3601 tgggttcccc cacagactcc cagcggctgg gataccgctg cacaaagtgc aaccggttca
     3661 cggctctacc cgtccgggac ctacattgca cggctggact acaatgtgaa ccacctgcag
     3721 gacaatactg agggaagtgc actcttctgc cgtcccctcc cggtacaggt tgcccttgct
     3781 tccgagcggg tatcggcctc atccggtgcc gccgaggtgg tcctgggcaa tccgttcacc
     3841 ataacaatct ccggggtccc gggagcggac tactacctct gggtgaagag cacaggaagc
     3901 atgagcggga acccgggaga tcaacccccc cagatcactc ccagccagga aggagtcctt
     3961 accgacccgg tgaatggtcc ctatgctatc ggctcgcatc agatctcgac acggcaggcc
     4021 atgacaatcc gggacgatgt tccccccgcc cctgcaaacg ggacgcccta ttatgcgctc
     4081 gtcaccctga caggaagcgg ggtccggacc gtccagtggc agacctcctc tgcgactgat
     4141 gaccgtccct acaccttcca ggtcgaacgg gtatcgcccg gcggggtatc attcgacgag
     4201 gtcagtatcg tggttgtccg gggatcggtc agcattggga caggccctcc ccggtccttc
     4261 ttcttgggag aacaggtcct cctcgcaggg acgaacacgg agtctgatac tgtctacctc
     4321 ttcatgaccg gtccgaatct cccttcagct ggcgggagtc tgaccaatcc ccgcattccg
     4381 gtgagaacgg ggcagccgga gagctttacc gctgtttctg ttgatccgga caatacctgg
     4441 gaataccggt ggttttccgg taacctaggg atcgatgcag ggacctatac cgtgtacgcc
     4501 gttgcagggc cggcttcccg aactgatctc tcgctctggc ggtactcgac cagctccttc
     4561 tcgtttgcac ggccgttcgt gacggcaggg atcccacaag cgacggttgc cagaggagac
     4621 ccggtggagg tcaggggcac cgcaaccggg aacccggacc ccggagtggc attatggatc
     4681 tttgggatca accggttcct gtacgcgacc gaggaggtca atgcggatgg ttcgttcacc
     4741 tacaccctga acgaagggga gaccacggat ctggcaaccg ggcagtatta cctggtaatc
     4801 cagcacccgg gatataccga tacctttgat gtgtatcctt ctcccaaccg gcagctggtc
     4861 ctctcttcat atcctgtccc gggaagcgcc ctcttccggg tggggggtcc gggggccctg
     4921 atgagctcgc aggcggccga tgcactggtc cggtcgctgg caagcccctt cattgatgac
     4981 acctacgtgg cgttgtcgtt tcttctcgtc aatccacagg taacgatcgc tcctcccggg
     5041 aactcgacga tcggtggcct gatcaacctg tccggaacga ccaatctcgc tccgggcaac
     5101 cagctcttgg tggaggtgac ctcacaggcc tttggaccct ctccaaaaga agggggagga
     5161 gcgttctcgg gggtctccgg gatcgcacca attcgggaag ggagtggtgg ccagaacaac
     5221 tggtccttct cctttgatac cgactcgttt gtcgccgata cctaccttgt cacggtcagc
     5281 ggggtgactg tttccaatgc cgttgccacc acctcatttt ccctgtcagc ggccaccccg
     5341 actcccacct cgtccacgcc agcaaccccc cagccaacga ttaccatacc aacgactgat
     5401 cccacccctt ttcctacaac aaccgccagc ctctcctggt tcgccctggc aggagcggtc
     5461 gttttcatcc tgattgcatt cgggagtgca gggaggaacg gctgaatttt tttaatgtgg
     5521 tttgccattt tttattttcg tgctcgtgtc tcaatccaac gcatgtattt tttaaaaaga
     5581 ggcaattatg aaccacacct tttcccggca cacaatggat gctcttcttc gagggaggga
     5641 atcggggagg aggttcatgg gaacggactt ttgcctgcga tgaggaggaa gtaagagcaa
     5701 ttcataactc ttttatgcat ggataacaac cgccccatag cagaagggag atgatgatga
     5761 gggcacggga ctatcacgaa ggcaggccga tcatgcatat gaatgccggt ccgggagctg
     5821 aaaacgtatg acccagtttg atccggtctt tcttgccatc gccaacctgg gagtgctgat
     5881 atttatcatt gcgagcatgc tcggcatggg gtttaacctg accattcctc agatccttgc
     5941 ccccctgaag aacaggaagc tcgtcatcat ggctcttgtg gccaactttg tagtagtgcc
     6001 tgtacttgca ctcctgatcg tgtggatcat cccgctctcc gagggattgc agatcggcct
     6061 gatccttgtc ggtttcgctg caggggctcc attcctcccc aagctggttc agctggccaa
     6121 aggcgatatg gcgtttaccg ccgggctgat ggtccttctg atggtgattt ccatcgcctt
     6181 cctgcccatc gttctgccat ttgtgctgcc cggagtgcag gtgagtccgt gggagatctc
     6241 aaagagcctg atcgtgctga tgctcatccc gctcgccctt gcccttttca tccgggcgag
     6301 gtacgaagag gtggcaacag gcctcgtcca cacgatgacc atggcagcca acctctccct
     6361 cgccgcaatg ttcatcggat actttgtcgc ttatttcgat gaaagctttg gcattcttgg
     6421 aaccggcgga gttctcgcgg cagtcctgct ggttatcgga gcggttctca caggatttct
     6481 gctgggtggg accaataaag agatcaaaaa ggtccttgcc ctcggaaccg gccaacgaaa
     6541 tctggctgcg gcatttgcca tcgcgaccag caattttgca gccaatcctg atgtagtact
     6601 ggaggtcatg gacgtggcag tgatcggttt tattctcctg atggtcattg ccggagaact
     6661 cgggaggcgc agcgcacgga gagcaggaaa ggggctgaag gccgcagtga gggattgatg
     6721 atatcccgcc gaagtgacga tgccagcgat cagggaagcc tgaacaccag gttgtgaacg
     6781 cgaagaacta aaaaaagatt agtaccgggg ctgccggtgc tttggcaggc attcctgaca
     6841 atacacgggg cgtccctcag tgggcttgaa cggtacttcg caatcctttc cacaatctga
     6901 acatactact tttgtcattt cgcggggccc ggtagttctc ggactcccat aatttgatcg
     6961 attatacatg atctttctca aaacagttat tggaaactgt tgtatgacac atgctgcgtg
     7021 agtatatcta gtattgcatc gtagcccctt gaaagagggg aatagtgtag gtcctgggtg
     7081 cagagcatga aggaagagga ccatgagcag caggtaaaga ctcctccttc acagagaaaa
     7141 ggttgttaaa agtcaacaag agtgcctgat gtcccttctc aatccatttc gtaattgtca
     7201 ggagcgcaga aataatcccc gccaatcctg aagagctccc ccccgcattc cgggcagaaa
     7261 tggggtcccg gcggaggagc gcagcaactg caggtattct tcaaaaaata ctcctgactg
     7321 cactgtgagc aacgataacg gtccatcgtg gtagatatca gtcttctctg ccttaaacct
     7381 gtcacaggat ttccctgctc cattcctctt cctatccggt actttcccct gcccggtgct
     7441 ttccctttgt ccgttacttt ctccgccccg gtacttcctc ccttttctat tccttttcct
     7501 ggtccgctcc ttttcctggt ctctacccaa aatccttttt tccctcctgg tccaacctga
     7561 acctcagatg tcaccagcca gatgttttct cattttcggc attgttcttg ccgcactcct
     7621 gattgcagca ggatgtacca ccgggcaacc ttccgtgcca gcttccacga caccaacaac
     7681 caccccggtt atcacaggga cgatgacagc cgctaccacc ccggttatga cgtcagttgc
     7741 cgttgagtca tggaaaaaag tacagcttga gacgaacatg gggacgatcg tgatcgccct
     7801 tgatccctcc atgccgataa cggccgggaa cttcgagacc ctcgtaaagg ccgggtacta
     7861 taacggggtg atattccacc gcgtgattga cgaattcatg atccagggag gagaccccac
     7921 cgggaccggg ggcggcggac cgggatatat gataccggac gagttccagc cggggaaccg
     7981 gaacgaccgc gggaccgtgg ccatggcaaa tgccggtccc aacactggcg gatcccagtt
     8041 cttcatcaac ctcgtca
//
LOCUS       DAOOCO010000077         8030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8468_length_8030_cov_5.942445, whole genome shotgun sequence.
ACCESSION   DAOOCO010000077 DAOOCO010000000
VERSION     DAOOCO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_8030_cov_5.942445"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1437
                     /locus_tag="PK154_05605"
     CDS             376..1437
                     /locus_tag="PK154_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10572.1"
                     /translation="MQLFPRFPAIFEKSIYLSAGTWRFMRYVRFIFCCLFLILATGCI
                     TQTPSSTDSTLQQGIEEIGDNGNNSPVPLTSGAPLTTQTESVQNLIYEKEGSAFDSIQ
                     ITIHNITVMQKSGSKFPVVVFNISLINEGLNEPFFLDDRSLVCFQSDFETTYPKLDDP
                     TMNITNPILLHGIGPGEQIVGEVGFFLNEHVDSLALYVRDPNWTILGGVYIPDMDNIT
                     QITSDREYPKNLTLSVPSAVQKATIPGANLRPGHKIAIINVSITNHNPTDVTIQREQL
                     LLLTERGMTLEHGGSRLTPEMAREYLRFPLTIHPGETKTGPVFIIVYSGTRTNKLALT
                     DKNFVIQSLIDLNGIYRYE"
     gene            complement(1522..1767)
                     /locus_tag="PK154_05610"
     CDS             complement(1522..1767)
                     /locus_tag="PK154_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10573.1"
                     /translation="MKKWVCGVPKTTGMNKTMMKNSIKPSVTGTRSGYVIRFTCPECH
                     HENAIIINMPKSYYKESRDGTCGKCRKHFNVLTPGQN"
     gene            complement(1947..2072)
                     /locus_tag="PK154_05615"
     CDS             complement(1947..2072)
                     /locus_tag="PK154_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10574.1"
                     /translation="MRRKRHQAVYDSTGSVSEQEAENAVQKAEDILHRIESIIHE"
     gene            complement(2115..2360)
                     /locus_tag="PK154_05620"
     CDS             complement(2115..2360)
                     /locus_tag="PK154_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10575.1"
                     /translation="MIEDLEREGFISPLPIDQNRIKDTLANAHRDLDVSYTLLSSSSD
                     WAFTIAYNTVLQAGRALMFSKGFRPAGSNQHISVVRF"
     gene            complement(2353..2916)
                     /locus_tag="PK154_05625"
     CDS             complement(2353..2916)
                     /locus_tag="PK154_05625"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPW10576.1"
                     /translation="MLELLIPSQTRITLLTLFLMNPGREYYIREIERTTGEHYAAIHS
                     ELSNLESLGLLSRRRRGNQVYFKVNQDFFLYHELQQIILKTEGISKVFREQLQNLEGI
                     SCLFLYGSYAAGTAGFKSDIDLFIVGDIDENAILTAIGAAEEELGREINYTLMTDEEF
                     RKRQQKGDPFVSNVMREPRVILAGCND"
     gene            complement(3049..3978)
                     /locus_tag="PK154_05630"
     CDS             complement(3049..3978)
                     /locus_tag="PK154_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPW10577.1"
                     /translation="MRCKIGYSPSIERSRPSDALHHAVLAEQLGFDSIWVDDHFLPVP
                     HMTECGFAWTWMSSALQATDQVFFATAVTAPIMRYNPAVVAHAFATMGAMYPGRVGIG
                     VGSGEELNEMAVVRGDWPPPGKRLDMLEEALSVMEKLWTSTAPTSFTGSYYTLANATL
                     FTRPEQHIPVYFSAIGPRASKMAGLLGDHLITVARNPSIVRDVIFPNFEAGARQAGKD
                     PATMERALAVLYYYDPDHLVDPEAMKVSASEAKIEVAGEGSQMELWHSAEDIIRRIEE
                     WKRLGFDHLIFGNFSGDCEAGLRVFEDVLPHVI"
     gene            4076..4255
                     /locus_tag="PK154_05635"
     CDS             4076..4255
                     /locus_tag="PK154_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10578.1"
                     /translation="MTSAQSPSETTHSLLPKACLGICGADFSPPSLVAIAQQRMIMWR
                     KKESPYNAVRYPPGI"
     gene            complement(4256..5767)
                     /locus_tag="PK154_05640"
     CDS             complement(4256..5767)
                     /locus_tag="PK154_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPW10579.1"
                     /translation="MKQLPLIGGVRGTTIDAVYEKTKMPPKIHPDHPLPVVGFSCMTR
                     EPTDEEIPGLIDAAIAQVLGPQGLPAIIRPGDRVVIKVNIVGPSQGLPGEKGRGIISD
                     PRIVRYVAGKVREIIGFGGTADLKVIDATFTPDKDPSDTKNPQSFHHARLQRSEGKAV
                     HETDVCYDYNADGILDGGSGAELVNLDAVPGSERFMTVFDEPIRGRSEVWFPKFLRTK
                     EQARGEADYCDLYIGIPILKSHGFCGMTGALKLHWGTMLGLLEKQEHAGYGFGTGDIR
                     LFLDYLCAMNRTRGFDLVIMDALTGNRCGPTNNADDPDVRTDFILMNAILCSKESVAI
                     DTVETLLAGFRLDSIPLLESAFRDGIGINKPAYIDLKGFEAFKHHKKWLRETYPGCGP
                     GSYPLQAGIGNARTHEDFNAPEGVTVSCRKADQHYVFSYTAKEPTPCNHNLARIDLLV
                     NGTVVRRLFENLTGGSVPVSLEQYWNTTVRYQIAAWDNALNCTLSAEKDMKVS"
     gene            complement(6159..>8030)
                     /locus_tag="PK154_05645"
     CDS             complement(6159..>8030)
                     /locus_tag="PK154_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10580.1"
                     /translation="MANADLLYVAYDGAAWQIQTPDATGTVGKYSSLRLDSSGTPRIS
                     YYDETNQDLKYAALNGPSWQVDNVDTSGVVGQYTSLVLDGAGNPQISYYNGTAGALKV
                     AHPPLPAPAVTAISPPNGVLGYTIAATITGTNFHPGASVRLSGAGYPDIWGTGVLVTS
                     QTTITCTFVIKATEPVDYRNVVVTNADGQSGALQNGFAILRQSAGGLNWAVETVDSAG
                     DVGWSTSLKLDQNGRPRISYLDWSNPALKYAAYNGSGWQITTVERPGDVWEGTSLALD
                     SAGNPRIAYHDQAAAALKYASYNGASWQISAVAPDGTFGGDASMVLDTATGNPRITYQ
                     DYASSLLKYASYNGSAWQIQSVGMNGYDCSLALDRSARPHISFNDVAGRLQYASWTGS
                     AWNIQTVDSRAGSGTSLVLDSADNPRIAYGDLIGGTMKYAEYDGQAWHISTLEGGYAG
                     LCTSLALDSKGYPCIAYTGPSDSSLKYAEYNGSAWNLQTLDIAGNTGLDLSLDLDESD
                     NVHISYRYMQSVTPTSCDLKYARGTRIVPGGPQPTVPIPNYTRPEIDRWNITRPEVEK
                     WNITPPTSAFPNVTRPEVEKWNVTPPTSAFPNVTRPEVEKWNVTPPTSSFPNVTRAE"
BASE COUNT         1777 a         2160 c         2125 g         1968 t
ORIGIN      
        1 cggaaaccag ggtattgaag ctggcaatgt cagcgatcgg tgccgagagc tcccgcactg
       61 cggtcttggt gacgctctgt tgaatgaaat ctcccatagt gtgttactcc tgccctcact
      121 ggtgagggcg aagaacagat accatggatc ctgttatcta tttttacccg ggatgtcgat
      181 cggggcgtaa aatggtccgg aaatcagaat cggagcgaat tgatgcgcac cagaattttc
      241 acggcggaaa aataggggtg gtctgacgtt aaaataacgt gtaattctgg gtggatgtat
      301 cgacaaattt tctgtgacag tccatttccg cagagtggga aaccgagtgt ttctaatccg
      361 ggaacaggat gagcgatgca gttgttcccc cggttccctg caatctttga gaagagcatt
      421 tatttgagtg caggtacatg gcgtttcatg cgatatgtac ggtttatttt ttgttgcctc
      481 ttcctcattc ttgcaacagg ttgcataaca caaacaccct cttcgactga ctcaactctg
      541 cagcaaggta tagaggaaat tggggacaat ggaaataatt caccagtccc cctcacgtca
      601 ggtgccccgc tcacaacaca aaccgaatca gttcagaatc ttatttatga aaaggaaggt
      661 tctgcatttg attccattca aataactatt cataatatca ccgttatgca aaaatccggt
      721 tcgaaatttc ccgtggtcgt gtttaatata tcactgataa acgaaggatt gaatgaacca
      781 ttctttttag acgacagatc gcttgtatgt ttccaatcgg attttgaaac tacctatccg
      841 aagctggatg atcccacgat gaatatcacc aatccaatcc tccttcatgg tatcggtcca
      901 ggtgaacaaa tagtcggaga agtgggtttc ttcttaaatg aacatgtaga ctctttggcg
      961 ttatatgtga gagatcctaa ctggaccatc ctcggagggg tctatatccc cgatatggac
     1021 aatataaccc aaatcacatc agaccgcgaa tatccaaaaa atctcacatt gagcgtccct
     1081 tctgccgtcc aaaaagctac gattcccggc gccaacctcc gacccggcca taagattgca
     1141 atcattaatg tcagtatcac gaatcataac cccactgatg tcacgatcca gagggaacaa
     1201 ctgttgttgc tgactgaacg gggaatgacg ttggagcatg ggggatcacg tctgacaccc
     1261 gaaatggccc gcgaatatct caggttcccc ctcacaattc accctggtga gacaaaaacc
     1321 ggacctgttt ttattatcgt atatagtggt acaaggacca ataaactcgc actcaccgat
     1381 aaaaattttg ttatccaatc gttgattgat ttgaatggaa tctatcggta tgaataatca
     1441 ttttgttggg ggatatcaca gcctggccaa atcgcaatcg aggtgatccg ccgggatgtc
     1501 accgggtaag ggagatgaac tctaattctg gcccggtgtc aggacattga aatgtttcct
     1561 gcattttccg caggtcccat ccctgctctc tttgtaatac gactttggca tgttgataat
     1621 aattgcattc tcatgatgac actccgggca ggtaaaacgg atcacatatc cggaccgcgt
     1681 tcctgttacc gatggtttta tggaattttt catcattgtc ttgttcattc cggtggtttt
     1741 tgggacgcca caaacccatt ttttcatatc cccgcaaacg gagatatatc gaagcatatc
     1801 ccgggatgac tcgttcccgg atgtcaaaaa aaaggcattg aaggtgcccc gattgtttcc
     1861 gacactggta tcattggtct tgtgagggga taaaagtgaa tgtacgatcg gcttgaaccg
     1921 ccaatcgata attccggatc cggcctctat tcgtgaatga ttgattcgat cctgtgcagg
     1981 atatcctctg ccttctgtac tgcattctcg gcctcctgtt cggataccga tcccgtgctg
     2041 tcatagacgg cctggtgccg tttccgtcgc atccgttcga accaccgggc atcatcacgc
     2101 gagaggaatt gctctcaaaa tcgtacgacc gagatatgct ggtttgatcc tgcaggtcgg
     2161 aaccctttcg agaacatcaa agcccttcca gcttgaagga cagtattgta ggcgatggta
     2221 aaagcccagt cgctgcttga ggagagaagg gtgtaagaaa cgtcaaggtc acggtgagcg
     2281 tttgccagag tatctttaat cctgttctga tcaatgggga gggggctgat gaacccttcc
     2341 cgttcgagat cttcaatcat tgcatcccgc caggattact ctgggttctc tcatgacatt
     2401 cgagacgaag ggatctccct tctgctgtcg tttccggaac tcctcatcgg tcatcagggt
     2461 ataattgatc tccctgccaa gttcttcttc cgctgcaccg attgcagtga gtattgcatt
     2521 ttcgtcgata tccccaacaa tgaagagatc gatatcgctc ttaaaaccag cagtgcctgc
     2581 cgcataggaa ccatagagga agagacatga tatcccctcc agattctgga gctgttcccg
     2641 gaataccttg ctgatccctt ctgtcttgag gatgatctgc tggagctcat ggtagaggaa
     2701 gaagtcctgg ttcaccttga aatagacctg gtttcccctg cgtctccggg agaggagacc
     2761 aagggattcc aggtttgaca gttcgctgtg aatggcggca tagtgctccc cggttgtccg
     2821 ttcaatctcc cggatataat attcccttcc cggattcatc agaaagaggg tgagcagtgt
     2881 gatgcgggtc tgggacggga tgagcagctc aagcatgata ataaaatata atcaattgat
     2941 taaaatagtt tatcatcaag aatgaggtgg gtatgaacac catgcttcag ctctctctgc
     3001 ccatgtagtt tattttcctc gtaatgagtg ttgcctgctg aactattttc agatcacgtg
     3061 cgggaggacg tcttcaaata ccctgaggcc tgcctcacaa tcaccactga agttcccaaa
     3121 gatgaggtga tcgaatccca gccgcttcca ctcttcgatt cgccggatga tgtcttcggc
     3181 gctgtgccag agctccatct gggatccctc cccggcaacc tcgatcttcg cttccgaggc
     3241 agagaccttc atcgcctcag gatccaccag atggtccgga tcgtaataat acagaactgc
     3301 gagggcacgt tccatggtcg ctgggtcttt tcctgcctgt cgtgcaccgg cttcgaagtt
     3361 cgggaatatc acgtccctga cgatagaggg attccttgca acggtgatca ggtgatctcc
     3421 caggagccct gccatcttcg acgcccgtgg tccgatcgcg ctgaaataca cagggatatg
     3481 ctgctctggc ctggtgaaca gggtggcatt ggcgagggtg taatacgatc cggtaaaact
     3541 ggtgggggcc gtgctcgtcc agagcttctc catgacggag agcgcctctt caagcatgtc
     3601 aagccgtttt cccggagggg gccagtcccc tcgcaccact gccatctcgt tcagctcttc
     3661 ccctgaaccg accccgatcc cgacccgtcc cgggtacatc gctcccatgg tggcaaaggc
     3721 atgcgccacg acggcaggat tgtaccgcat gatcggggcg gtgaccgcag tggcaaagaa
     3781 tacctggtcc gtggcctgga gggcagagct catccatgtc caggcaaatc cgcattcggt
     3841 catgtggggt accggaagga agtgatcatc gacccagatc gaatcaaatc ccaactgttc
     3901 agcgagtact gcatggtgta acgcatcgct cggccgggac cgttcgatac tggggctgta
     3961 tcctatttta catcgcatgg agatcctcgt tcaatgatct ccacaggaag caaaaagctt
     4021 ctcgtttttt ggatggacga aattcccctc ccggctgttt cgaggatccc ggttgatgac
     4081 atcggcacaa tcgccatcag aaacaaccca ttcactgctg ccgaaggcgt gccttgggat
     4141 ctgtggagcc gatttttccc ctccctccct tgtggcaatt gctcaacagc gaatgataat
     4201 gtggagaaaa aaagaatcgc cctacaacgc cgtgcgctat cccccgggaa tctgatcagg
     4261 acaccttcat gtccttctca gccgagagcg tgcagttgag ggcgttatcc caggcagcaa
     4321 tctgataccg cacagtcgta ttccagtact gctccaggct gaccggaaca ctgcctcctg
     4381 tcaggttttc aaagaggcgc cggacgactg tcccgttcac aaggagatcg atccttgcga
     4441 ggttgtgatt gcagggcgtt ggctcctttg ccgtgtagct gaacacatag tgctgatcag
     4501 ccttcctgca ggagacagtc acgccttcgg gggcattgaa gtcctcgtgg gtcctcgcat
     4561 tccctatccc tgcctggagc ggatagctcc ccggaccgca gccagggtag gtctcccgca
     4621 gccatttctt gtggtgtttg aaagcctcga agcctttcag gtcgatatat gccggcttgt
     4681 tgatcccgat cccgtcacgg aatgccgatt cgaggagggg gatcgagtcc agccggaacc
     4741 cggcaagcag cgtctcgacc gtgtcgatgg caaccgattc cttcgaacag aggattgcat
     4801 tcatgaggat gaaatcggtc ctgacatcag gatcatcggc attgttcgtc gggccacaac
     4861 ggttcccggt aagggcatcc atgatcacca gatcgaaccc ccgggtccgg ttcatcgcac
     4921 acaggtaatc aagaaacagg cggatatcgc ctgtaccgaa accatagccg gcatgctcct
     4981 gtttctcgag gaggccgagc atcgttcccc agtggagttt gagagccccg gtcatgccgc
     5041 agaacccatg gcttttcagt atcgggatcc cgatatacaa atcgcaatag tctgcctctc
     5101 cccgggcctg ctcctttgtc cggaggaact ttggaaacca cacctcgctc ctccccctga
     5161 ttggttcgtc gaaaacggtc atgaaccgct ccgatcccgg cactgcatcg aggttgacga
     5221 gctctgctcc ggaacctcca tcgagtatcc cgtcggcatt gtaatcgtag cagacatcgg
     5281 tctcatggac ggccttcccc tcactccgct gtaagcgggc atggtgaaaa ctttgtgggt
     5341 tcttcgtatc cgagggatcc ttatcaggag tgaaggtggc atcgatgacc ttcaggtccg
     5401 cggttccccc gaatccgatg atctcccgga ctttgccggc gacgtatctc acaatacgcg
     5461 ggtctgagat gatcccacgc cccttctccc ctggcaagcc ctggcttggc ccgacgatat
     5521 tgactttgat cactaccctg tctcccggcc tgatgatcgc cgggaggccc tgcggtccca
     5581 gcacctgtgc gatggcagca tcgatcaggc ctgggatctc ttcatcagtg ggctcccgtg
     5641 tcatgcagga aaaccctacg actggtaagg ggtgatcggg gtggattttc ggcggcatct
     5701 ttgttttttc gtacactgca tcgatggttg tcccccggac acccccgatc agggggagct
     5761 gtttcatatc attgtgaatt ttctcccatc ggcgactgac atcgctggaa taatgaacac
     5821 cccccggagg ggatatgctg cccgcatcgg agcaggtgtc cgatacagga tcatcagacg
     5881 gattcatgat agcaagcctc ttcgatcagc aattggatca tcatcattaa taccttccgg
     5941 aaatcaatct gacgtggtta taattccccc accctatcgg ttggggcggg taaagcgcca
     6001 tggattcctg ccaaggtcgg ggggtgacat tccccttttc tgcctccggc acagtaccat
     6061 tccaatctgc tattctgccc gggtcacatt gggaaatgag gaggtcagag gggtgacatt
     6121 ccccttttct gcctccggca cagtaccatt ccaatctgct attctgcccg ggtgacattt
     6181 ggaaatgagg atgtcggagg agtgacattc cacttttcta cctccggcct ggtgacattg
     6241 ggaaatgcgg aggtcggagg ggtgacattc cacttttcta cctccggcct ggtcacattg
     6301 gggaatgcgg aggttggagg ggtgatattc cacttctcta cctccggccg ggtaatgttc
     6361 caccgatcta tctcaggccg ggtataattc gggatgggga ctgtcggttg gggaccgccc
     6421 ggcacgatac gcgtccctct ggcatacttc aggtcgcaac tggtgggggt taccgactgc
     6481 atgtaccggt aactgatgtg tacgttgtcg ctctcatcga ggtcgagcga tagatcaagg
     6541 ccggtgttgc cagcgatgtc gagggtctgg agattccacg ctgaaccgtt gtactccgcg
     6601 tatttcagcg atgaatccga aggtccggta tacgcgatgc agggataccc cttgctgtcc
     6661 agggcaaggg atgtacacaa cccggcatat ccgccttcaa gggtagaaat gtgccacgcc
     6721 tggccgtcat actccgcata tttcatagta cctcctatca ggtcaccata ggcgatacgt
     6781 gggttatcgg cactgtcaag caccagcgag gtaccggaac ctgcccggga atcgacggtc
     6841 tggatgttcc aggccgagcc ggtccaggaa gcatactgga gccttcctgc aacgtcgttg
     6901 aagctgatat ggggtctggc ggaacgatcc agcgcaaggg agcagtcata cccgttcatc
     6961 cccactgact ggatctgcca tgctgatccg ttatatgacg cgtatttcag gagtgaactt
     7021 gcataatcct ggtaggtgat ccgcgggttt ccggtcgctg tatccagtac catggacgca
     7081 tccccgccaa aagtgccatc aggggcaact gcggagattt gccacgaagc accgttataa
     7141 gaggcgtact tgagggccgc tgctgcctgg tcatggtagg cgatccgcgg attcccggca
     7201 ctatccagtg ccagtgacgt tccctcccag acatcgccgg gtctctccac ggtggtgatc
     7261 tgccagcctg atccgttata ggccgcatac ttcagggcag gattggacca gtcgaggtag
     7321 ctgatgcggg gtctgccgtt ctggtccagt tttagggatg tgctccagcc aacatccccc
     7381 gcggaatcca ccgtttcgac tgcccagttc aggccccccg cactctgcct gaggatggca
     7441 aagccattct gcaatgcccc ggattgccca tcggcattcg tgacaacgac gttccggtag
     7501 tcaaccggtt ctgttgcttt gatgacgaag gtgcaggtga tcgtggtctg ggaggtgacc
     7561 aggacccctg ttccccagat gtccggatac cctgccccgg aaagccggac cgatgcaccg
     7621 gggtggaaat tggtgccggt gatggttgcc gcgatcgtgt atcccaggac cccgttcggt
     7681 ggactgatgg cagtgacagc gggtgccgga agcggcggat gggcgacctt gagggctcct
     7741 gcggtaccgt tataatagct gatctgggga ttcccggcac cgtccagcac cagggaggta
     7801 tactgcccga caacacctga ggtatcgaca ttgtcaacct gccaggaggg gccgttcagg
     7861 gctgcgtact tcaggtcctg gtttgtctcg tcgtagtaac tgatacgggg agtgccggag
     7921 ctatcaagcc ggagcgagct gtacttgccg accgttccgg tagcgtcggg ggtctggatc
     7981 tgccaggctg caccgtcata tgccacatag agcaggtccg cgttggccat
//
LOCUS       DAOOCO010000078         8017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8494_length_8017_cov_4.159633, whole genome shotgun sequence.
ACCESSION   DAOOCO010000078 DAOOCO010000000
VERSION     DAOOCO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8494_length_8017_cov_4.159633"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK154_05650"
     CDS             complement(<1..356)
                     /locus_tag="PK154_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10581.1"
                     /translation="MHDDTFRSDLDREYPCLLELYKDLHAHPELSGQEAWTSDRVGKE
                     LEAAGFVTTRGVGGYGVVGVLTRGTGPVVMVRADMDGLPLKEETGLPYTSREKTHDAR
                     GQEVHVMHACGHDVHMA"
     gene            complement(349..1173)
                     /locus_tag="PK154_05655"
     CDS             complement(349..1173)
                     /locus_tag="PK154_05655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW10582.1"
                     /translation="MPAPYYTRGDVTLYLGDCIALMEAMPEESVDLVFADPPYNLSNG
                     GFTCHSGRRAPVDKGAWDSSNGIEEDFYFHGEWIAACKRVLKETGSIWISGTYHSIYA
                     CGFALQRLGYHILNDVCWFKPNAPPNLSTRYFTASHETLIWAKKSRDVPHTFNYSLLK
                     NADWACDPFKKPGRQMRTVWSIPSPRGSEKKCGRHPTQKPYALLERIILGCTVQGSLV
                     LDPFTGSSTTGLAADATGRRFVGIDREPEYLDLSIARFEDRARKQAGSRAGGAGDA"
     gene            1372..1974
                     /gene="hxlB"
                     /locus_tag="PK154_05660"
     CDS             1372..1974
                     /gene="hxlB"
                     /locus_tag="PK154_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HPW10583.1"
                     /translation="MAARHSVQDMMRLMASKIRDTAASLSDSETEAFLSAIQNAERIY
                     VMGAGRSGLVAKAFAMRLMHLGFISFVVGETITPAMQQKDLLVIFSGSGRTKTIADIA
                     ETAKEIGGRIALITSNRDSKISRMADVIVIIENQRDQVRDETAEFEIRQMTGEHKSFA
                     PLGTLFETAAMVFADACISRLMEVSMIDEKELKNRHANIE"
     gene            1984..3093
                     /locus_tag="PK154_05665"
     CDS             1984..3093
                     /locus_tag="PK154_05665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW10584.1"
                     /translation="MKEYVCAERCRGIEISGIRKLFESARPDSIDLGLGQPDFDTPDH
                     VKEGAIQAIREGKTGYTSNMGIPELREALSTKFKKENGLSYDPGQIIVTAGASEALHI
                     IMQALVGEGDRVVFADPGFVSYGALAVLAGGRPEGIPLDAKLHLDIEAAKSLMDGARL
                     IVINSPGNPTGAVESRESIQAIAEYAEDAGVTVVSDEVYEHFIYGPSHHSPATYSDDV
                     ITVNAASKTYAMTGWRLGYLAGPKEYTEQCLKVHQYCQACASSISQYAALAGYTGPQE
                     CVHQMRDEYRRRRDYLYGALREMGFSFPLPEGAFYLFVPMKREILAKVLEKGVIIVPG
                     SAFGCNAPDYARISYAASMEKLKTAVERIRAAVGE"
     gene            3097..4134
                     /locus_tag="PK154_05670"
     CDS             3097..4134
                     /locus_tag="PK154_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPW10585.1"
                     /translation="MVKDLLMKLSNAHGLSGSEGSVLSIVRKELKNHVDELHEDAMGN
                     LIAVKKGNDFKVMIAAHSDEIGLMVKSIDDKGFIRFVTLGGWYAPTLYNQRVILHGTS
                     GKIPGVLGGKPPHKMDEEERKKGVKTDDMFIDIGATSREDAEALGIEVGTPGTIDREV
                     VELGNGRLTGKAFDNRVGVAMLISALQKVRSPFTIYGVFTVQEEVGLKGAKTSAYTLN
                     PDCAIATDVTIPGDHPGIDKNDVPVEMGMGPVITIVDASGRGLIASRAMVQWLKKTAK
                     ACDIPVQVEVGAGGTTDATAIHLERGGIPSTTLSITTRYIHSPVEVVDPRDIEAGVAL
                     LVEALKTKPRL"
     gene            4911..7964
                     /locus_tag="PK154_05675"
     CDS             4911..7964
                     /locus_tag="PK154_05675"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW10586.1"
                     /translation="MNMKGVMCTVILLLAATLLVLPVSGAFNVTGINPDRGNNTALPV
                     LVDITGNDLPNQVIVKLVNLSLSSNITASSAIWIDSTRIVSVFDLNGKVAGERAVVVV
                     NKSDGSEAVLEGTPFLVENLPPTVSSVTPNSGINTQDSVDLTVKGSDFRDGDLKVTLT
                     KESTTPISAEGVSWVSSGEITCKVNVSGAAAGKWSVEVTNPDGKTGALDEGFTVWNPA
                     PTVTKITPSGDSNNKPVVSCTVEGTGFIVGASVNLSMAGQPDIKGYIAIVTSTAISCS
                     FDLTDVQVGAWDVVVTNEDGQSGTLPAAFLITYPVAPVVDTIEPATGVNSGIVTITNL
                     SGTGFEEGATVVFTKDLATIAAENVIVNSPVNITCSVNLTGAGIGQWNITVTNDDGQS
                     GFLDNAFEVVYPAPEIAGISPPSGANNVVVPVVISGAFFKDAPAVNLTKEGSTTIFGT
                     NVTYVNAGTIKCDLPLAGATLGNWSVTVRNPDGQVSNSLNFTVENPRPTVSGVTPSKG
                     TNDTVVAVTITGAYFLDSPRVPKVNLTMGTDVIQAENVVVNSPVNITCSVNLTGRALG
                     QWNLIVVNPDGKTGGLRNAFRITTAPPVPEFTASPTYGTVPLTVQFTDLSTNYPYIWS
                     WNFGDGSIVTGINQANPVHVYNTPGVYNVTLYALNSDGVGEKITKSSYITVVETPVAH
                     FTATPMSGPAPLFVQFTDTSDGDPYRWVWKFGDGQISDQKNPYFLYKNPGLYQVELTV
                     YNKAGSDTATKTIEVLVSPPDAEFTANRTAGDSPLTVRFTDLSTGAPTNWSWKFGDSG
                     SSEEQNPVYVFSSPGTYGVQLTASNAAGTSTETKNGYIVVGQTIKADFNITPSNEGQT
                     APLSVAFSDLSSGNVLKWNWNFGDAHISTERNPIHTYPSPGTYTITLSVLGLTGSDSV
                     TKTITVVAPLIADFIAEPTTGSAPLTIVLSDRSTGDVATREWVIMDNPEEYAKNIVYF
                     SEGQGDQMYTLTEPGLYTVRLTIRDADGKPDSKIKTNYINVLPFP"
BASE COUNT         1902 a         2135 c         2245 g         1735 t
ORIGIN      
        1 gccatgtgca catcatgccc gcaggcatgc atcacgtgta cctcctgacc acgggcatca
       61 tgcgtcttct ccctgctcgt gtagggaaga ccggtctcct ccttcagggg aagaccgtcc
      121 atgtctgccc gcaccattac aacgggaccg gttccgcggg tgagcactcc gaccacacca
      181 tacccgccaa ccccccgtgt cgtcacaaaa cctgccgctt ccagctcctt tcctacccga
      241 tccgacgtcc atgcctcctg tccggatagt tcggggtggg catgcaggtc cttgtacagt
      301 tcgagaaggc aggggtattc ccggtcgaga tctgaccgga acgtatcgtc atgcatcacc
      361 agcacctcct gccctgctcc cggcctgttt acgggcccgg tcttcaaaac gggcgatgga
      421 gagatcaaga tactccggtt cgcggtcgat gccgacaaac cgcctcccgg ttgcatccgc
      481 agcaaggccg gtggtggagc tgccggtgaa aggatcgagc accaggctgc cttgtacagt
      541 acatccaagg atgatgcgtt cgagaagagc atacggtttt tgcgtcggat gtcttccaca
      601 cttcttttca gaacctctgg gagaggggat actccatacc gtcctcatct ggcggccggg
      661 tttcttgaac ggatcgcatg cccagtccgc gttcttcagc agggagtagt tgaacgtgtg
      721 cgggacatcg cggcttttct tggcccagat gagcgtctcg tggctcgccg taaaataccg
      781 ggtgctcagg ttcggggggg catttggttt gaaccagcag acgtcgttca ggatatggta
      841 cccgagccgc tggagggcaa acccgcatgc atagatggaa tggtaggtgc cactgatcca
      901 gatcgatccg gtctctttga gcactcgttt gcaggcggcg atccattcac catggaagta
      961 aaagtcctcc tcgatcccgt tgctcgaatc ccaggccccc ttatccaccg gtgcccttct
     1021 ccctgaatgg caggtgaacc cgccgttcga caggttatac gggggatctg caaagaccag
     1081 atccactgat tcttccggca tcgcctccat gagggcgata cagtcgccga gatagagcgt
     1141 gacatcgcct cgggtgtagt agggtgcagg catcaaatac ggttcacatc gctcatgctg
     1201 ttcagaggtt catgctgttc tgaggatacg gggcatggat caaacgtctc tcctttccta
     1261 atcggtcgtg taaagaatag tatcccgaaa ggggcataaa tccctttcac cgcctatgga
     1321 gccacccata cgaaattgta tgtagtttgc agtgatattc tccagtgcaa aatggcagcc
     1381 aggcatagcg ttcaggacat gatgcggctc atggcatcaa agatcaggga taccgcggcc
     1441 agtctctccg acagcgagac cgaagcgttc ctctccgcga tccaaaatgc ggagcgcatc
     1501 tatgtgatgg gtgcagggcg gtcagggctt gttgccaaag catttgccat gcggctgatg
     1561 cacctcgggt tcatctcttt cgtggtcggg gagaccatca cccctgcaat gcagcagaag
     1621 gatctcctcg ttatcttctc cggttcaggc aggacaaaga cgatcgccga tattgccgag
     1681 acggctaagg agatcggggg ccgtattgcg ctcatcacgt ccaaccggga ctcgaagatc
     1741 agcaggatgg cagatgtgat cgtgatcatc gagaaccaga gagaccaggt cagggacgag
     1801 acggcagagt ttgagatacg gcagatgacc ggggagcaca aatcttttgc cccgctcggc
     1861 accctgtttg agaccgcagc gatggtgttt gccgatgcct gtatctcacg cctgatggag
     1921 gtctccatga tcgatgagaa ggagctgaag aaccgccatg cgaacatcga gtgaggagca
     1981 agtatgaagg agtatgtatg tgcagaacgg tgccggggga tcgagatatc cggtatccgg
     2041 aagttgttcg agtcagccag gcctgattcg atcgatctcg ggctggggca gcccgatttc
     2101 gacacccccg atcacgtgaa ggaaggggcg attcaggcca tcagggaggg aaagaccggc
     2161 tatacctcca atatggggat cccggaactc cgggaagccc tctccaccaa gttcaaaaag
     2221 gagaacggcc tctcgtatga tccaggccag atcattgtga ctgccggggc gagcgaagcc
     2281 cttcacatca tcatgcaggc gctggtcgga gaaggtgacc gcgtggtctt tgctgacccg
     2341 gggttcgtct cctacggggc acttgccgtt cttgcagggg gcaggccgga gggcatcccc
     2401 ctagacgcga agctgcacct cgacatcgag gctgccaagt ccctgatgga cggtgccagg
     2461 ctgatcgtga tcaattcacc gggcaacccg acgggagcgg tggagagccg ggagtcgatt
     2521 caggccattg ccgaatatgc tgaggatgcc ggggtgaccg tcgtgagcga cgaggtctac
     2581 gaacacttca tctatggacc atcccaccat agcccggcca cgtacagcga tgatgtcatc
     2641 accgtcaatg cggcaagcaa gacctatgcc atgaccgggt ggcgcctcgg ctacctggcg
     2701 gggccgaagg agtacaccga gcagtgcctg aaagtccacc agtactgcca ggcctgcgcg
     2761 agctcgatct cgcaatatgc agcccttgca ggatacacag gcccgcagga atgtgtccat
     2821 cagatgaggg atgaataccg gagacggagg gattacctct atggagcact ccgggagatg
     2881 ggattttcgt tccccctccc tgagggtgct ttctacctgt tcgtgccgat gaagcgggag
     2941 atcctggcca aggtcctgga gaaaggggtc attatcgtac cgggcagtgc attcggctgc
     3001 aatgcacccg actacgcccg catcagctac gctgcgtcga tggagaaact gaagactgcc
     3061 gtggagagaa tccgggcagc ggtaggggag tgaaccatgg taaaagatct gctgatgaag
     3121 ctctcgaatg cacacggtct ctcgggcagc gaggggagcg ttctttccat cgtcaggaag
     3181 gaactgaaga accatgttga tgaactgcac gaagatgcga tgggcaacct gattgccgta
     3241 aagaaaggga atgatttcaa ggtgatgatc gcggcccact ctgatgagat tgggctgatg
     3301 gtcaagtcca tcgatgacaa gggattcatc cggtttgtca ccctcggggg atggtatgct
     3361 cctaccctct acaaccagcg tgtgatcctc catggcacaa gcggtaaaat accgggtgtt
     3421 ctcgggggaa aaccccctca caagatggac gaggaggagc ggaagaaggg ggtaaagacc
     3481 gatgacatgt tcatcgacat cggggcgacg agcagggagg atgcggaggc gcttgggatc
     3541 gaggttggga ccccgggaac gatcgaccgc gaggtggtgg aactcggaaa cgggcggctc
     3601 accggaaagg ccttcgacaa ccgggtgggg gttgcgatgc tcatctctgc cctccagaag
     3661 gtcaggtcac cattcaccat ctatggggtc tttaccgtgc aggaagaggt tgggctgaag
     3721 ggggcaaaga cgagtgcgta caccctcaac cccgactgtg cgattgcaac cgatgtgacg
     3781 atccccgggg accatccagg catcgacaag aatgatgtgc cggtcgagat ggggatgggg
     3841 cctgtcatca cgatagtcga tgcaagcggc cggggtctca ttgcaagcag ggctatggtc
     3901 cagtggctga aaaagacggc aaaggcatgt gacattcccg tccaggtcga ggtgggtgcc
     3961 ggcgggacca ccgatgccac cgctatccac ctcgaacggg gtggcattcc gagcaccacg
     4021 ctctcgatca cgacccggta tatccattcc ccggtagagg tcgtggatcc cagggatatc
     4081 gaggcgggtg ttgccctgct tgtcgaagcc ctcaagacaa aaccacgact ctgattagag
     4141 aacggggaag acccgactct ttttttgcgg actgtcctgt agagggctgt aacgacccac
     4201 cgttttccta cgggggcgat atagattgat gatcaacgag ggcatgattg ttggattctg
     4261 gcaggattca atcggcagtg cttcgaaacg agtttacttc tttattgaca atcatctccc
     4321 tatcatcata tgctcaatct gaaggataga aaacccagat ccggacatat tccttgctaa
     4381 tttctatcgg atcaggtaaa ggtccagtgc cagttaagct gatttccatt catggtgcgg
     4441 gataattttt cctgaatatg atatgttttc agttatttcc tggattgact gtaccctggt
     4501 gagtatgcat tattctgatt tatgagagtt tttacgggga atgaagagca aatattcgtg
     4561 caatacggaa tcgcagtgtc ctcctttttt tgtgaacttg tttaccttgt taagctgaaa
     4621 gtatgttaat cccaaaaatt gtacagggcc ctgaaaaaag gtaaccctat catgtccagt
     4681 catgaaatat catgatgccg cagtgagatc atttacattg aagttttaac cttcattgaa
     4741 gtgtattatg gtaaaataac cccgcaaaac acctcatttt ttgggaaagg tttatatata
     4801 ttacgataga gcatatgatg aatacattta tatcatggaa ttgggcgcca aatcagtctg
     4861 tgtttcatga ttgggagatg tatacaaagc atatatgctg aggtgttcgt atgaacatga
     4921 aaggggtaat gtgtactgtc attctgctac tggcggccac cctgctggtg ctccctgttt
     4981 cgggcgcctt caatgtgacc ggcatcaacc ctgatagagg gaacaatacc gcgttgccgg
     5041 tattggtaga tataacggga aacgatcttc cgaaccaggt gatcgtaaaa ctggtgaatc
     5101 tgtcgctctc atcaaatatt acagcatcat cggcgatatg gattgattca acaagaatcg
     5161 tctctgtctt tgacctgaac ggcaaggttg ccggtgagag agcggtagtc gttgtgaaca
     5221 agtccgatgg gtctgaagcg gtacttgagg gaaccccgtt ccttgtggag aatcttccgc
     5281 caaccgtgtc atcggtgacg ccgaattcgg gtatcaacac ccaggacagt gttgatctca
     5341 ccgtgaaggg aagtgatttc cgtgatgggg acctcaaagt caccctcacc aaagagtcga
     5401 ccacacccat ctctgcagaa ggagtaagct gggtgtcatc aggagagatc acgtgcaagg
     5461 tcaatgtctc gggtgctgct gccgggaaat ggtcggtgga agtgaccaat cccgatggga
     5521 agaccggggc gctcgacgaa gggttcacag tctggaatcc cgcaccgact gtgacaaaga
     5581 taaccccgtc gggagattcc aataataaac cggttgtaag ttgcacagta gagggaaccg
     5641 gatttattgt tggagcatcg gttaatcttt ccatggccgg gcagcctgat ataaaagggt
     5701 atatcgcaat tgtaacatcg acagctatct cctgctcctt tgatcttacc gatgttcaag
     5761 tcggtgcttg ggacgttgtg gtgaccaatg aagatggaca atcgggaact ctccctgcgg
     5821 ccttcctgat cacctatcct gtggccccgg tcgttgatac cattgaaccg gcaacgggag
     5881 taaacagtgg aatcgtcacg atcacgaacc tctccgggac cggatttgaa gaaggggcaa
     5941 cggtcgtatt tactaaagac ctggcaacca tcgcagccga gaatgtcatc gtgaacagcc
     6001 cggtgaatat cacctgcagt gtgaacctga ccggcgctgg gatcgggcaa tggaatatca
     6061 ctgtcaccaa tgacgatggg cagtcaggat tccttgacaa tgcatttgaa gtggtctatc
     6121 cggcgcctga aattgccggc atctccccgc catcgggagc caataacgtc gttgtccctg
     6181 tggtcatatc cggggccttc ttcaaggatg cgcctgcagt gaacctcacc aaagaggggt
     6241 ccacaaccat cttcggcacc aatgtgacct atgtgaatgc cggcaccatc aagtgtgacc
     6301 tgcctcttgc gggtgcaacg cttgggaact ggagtgtaac cgtaagaaac cctgacgggc
     6361 aggtctcgaa ctccctcaat ttcaccgtgg agaacccgag gccgaccgtc tcgggggtca
     6421 caccctcaaa gggtaccaat gatacggtgg tcgctgtcac gataaccggt gcctacttct
     6481 tagattcacc gagggtccca aaggtcaacc tgacgatggg gaccgatgtc atccaggccg
     6541 agaatgtcgt ggtgaacagc ccggtgaata tcacctgtag tgtgaacctg accggaagag
     6601 cactcggcca gtggaacctg atcgtggtga accccgacgg taagacaggg ggattacgca
     6661 atgccttcag gataacgacc gctccgccgg ttcctgaatt cacagcaagc ccgacctatg
     6721 gcaccgtccc cctgacggtc cagttcactg atctctccac gaactacccc tacatctggt
     6781 catggaattt cggggacggt tcaatcgtca ctggcatcaa ccaggcaaac ccggtccatg
     6841 tgtataacac ccccggggta tacaatgtga cgctgtacgc actgaacagc gatggtgtcg
     6901 gggagaagat tacgaaatca tcgtatatca ccgtggttga aaccccggtg gcacacttca
     6961 cggcgacccc gatgtcggga ccagccccgc tcttcgtcca gttcaccgac acatccgatg
     7021 gtgatcccta ccggtgggta tggaagttcg gtgacgggca gatctctgat cagaagaacc
     7081 cctacttcct ctacaagaac cccgggctct accaggttga actgaccgtc tataacaagg
     7141 cgggatccga taccgcaaca aagacaatag aggtacttgt ctcaccaccg gatgctgaat
     7201 tcactgcgaa ccggaccgca ggagacagtc ccctgactgt caggttcacg gacctctcaa
     7261 caggagcacc caccaactgg tcatggaagt tcggggactc cggcagctcg gaagagcaga
     7321 acccggtata cgtgttctca agccccggaa cctatggtgt ccaactgacg gccagtaacg
     7381 cggctgggac cagcactgag accaagaacg ggtatatcgt ggtcggtcaa accataaagg
     7441 ccgacttcaa catcactccc agcaatgagg gtcaaacggc cccgctcagt gtggctttca
     7501 gcgatctctc cagcgggaat gtcctcaagt ggaactggaa ctttggtgac gcccatatct
     7561 caacggagcg aaatccaatc catacctatc cctcccctgg gacctatacc atcaccctgt
     7621 cagtgctcgg tcttacaggg agtgactcgg tcacaaagac catcacagtc gtagcccccc
     7681 tgatagcgga tttcatcgcg gaacccacga ccggttcagc accattgacg atcgtgctct
     7741 ctgaccgatc gaccggcgat gttgccacca gggaatgggt catcatggac aacccggaag
     7801 agtatgcaaa gaatattgta tacttctctg aaggacaggg cgaccagatg tacaccctca
     7861 ccgaacctgg cctctacacc gtcaggctga ccatccggga cgccgacggg aaacctgact
     7921 caaagataaa gacaaactac atcaatgtcc tccccttccc gtaaaatctc ggggggatcc
     7981 ctcagctttt ttttataaaa taacatgatt cctttga
//
LOCUS       DAOOCO010000079         7986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8533_length_7986_cov_5.482159, whole genome shotgun sequence.
ACCESSION   DAOOCO010000079 DAOOCO010000000
VERSION     DAOOCO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_7986_cov_5.482159"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="dapA"
                     /locus_tag="PK154_05680"
     CDS             <1..579
                     /gene="dapA"
                     /locus_tag="PK154_05680"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW10587.1"
                     /translation="DGVLVISPYYNKPNRSGLVKHYTILADLDIPVIVYNVPGRTGQN
                     LPPDLVVELAKHPNIVGIKEASGDIGQISRIIEGTLDEEFQVISGDDNLTFPILALGG
                     SGVISVAANVEPGRMVAMFEAFCESDFETALDLHYELSPLFRSMFIDTNPIPVKKAVE
                     LRGMAAGSVRLPLDELDHEKTSRLREVLASYD"
     gene            572..1333
                     /gene="dapB"
                     /locus_tag="PK154_05685"
     CDS             572..1333
                     /gene="dapB"
                     /locus_tag="PK154_05685"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPW10588.1"
                     /translation="MIKVVVCGALGRMGTTVGRLANEAPDMELVGGIDLRGGSFFGVD
                     VTEAAQIDTLLASARPDVLIDFTAPAASVSHVRAAAKSRTAVVVGTTGISPEQRAEME
                     KAIKGTIPAVISSNFSIGVNIFWNLLREAARMLPEYDVEVIEAHHRYKKDAPSGTAKT
                     ILQILDEELGLREKKYGREGMMERGREIGVHVIRGGDIVGDHSVLFSGNYETITLSHR
                     AYDRSVFAHGALKAARWVSGKKPGIYGMAEVLDLK"
     gene            1397..1570
                     /locus_tag="PK154_05690"
     CDS             1397..1570
                     /locus_tag="PK154_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW10589.1"
                     /translation="MTAVVDKDKCTACETCIDECPAAAISLNDGKAKVDKDLCVDCGS
                     CVDVCPSEAITLE"
     gene            1615..2631
                     /gene="asd"
                     /locus_tag="PK154_05695"
     CDS             1615..2631
                     /gene="asd"
                     /locus_tag="PK154_05695"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPW10590.1"
                     /translation="MINVGVLGATGAVGQRFVQLLAEHPWFNLTTLTASERSAGKKYR
                     DVVKWRLDVPFPDNIGDIRVSPTNVASLKEVDLVFSALPAEIAKDVETACANGGLAVC
                     SNASSHRMEPDIPLVVPEVNPDHLALIDVQRDHGRDGFIVTNPNCSTIVMTLALAPVH
                     GCSFQKIHVATMQAVSGAGFEGVPAMGIYDNVIPYIGQEEQKMETETLKIMGTFRNNA
                     VQPAPFQVSASCHRVPVLDGHTMAIWLDTREPVEKVKEAFLRYKPPIEGLPTQPKESV
                     HFFDEPDRPQPRLDRNRGNGMTVSVGRLREGIRFIAMGHNTIRGAAGASVLNAELISS
                     RKYL"
     gene            2642..2917
                     /gene="albA"
                     /locus_tag="PK154_05700"
     CDS             2642..2917
                     /gene="albA"
                     /locus_tag="PK154_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HPW10591.1"
                     /translation="MLKDNTVFVGNKPVMNYVLAVVTQFNNGASEVAIKARGKAISRA
                     VDTAEIALNRFLENVSKKEIITSTEIIDTDSGKTNVSSIEIVLVNQR"
     gene            3085..3504
                     /locus_tag="PK154_05705"
     CDS             3085..3504
                     /locus_tag="PK154_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10592.1"
                     /translation="MLLAVLTVPVFATTNTDKYGYITIEAVDITLDGGKATIDMKYTL
                     DEPTRVIVLLLGKQDLKNRLLTVLNYEDAEVKNLDLNSAKLVVDDISYSYGRGVYWFP
                     SHTFNVLIPTLRITTPQVSREYAAVTEIPNGIGYFAS"
     gene            complement(3741..4868)
                     /locus_tag="PK154_05710"
     CDS             complement(3741..4868)
                     /locus_tag="PK154_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HPW10593.1"
                     /translation="MQTGRTLVLCVDRDDDIGYKGRVESPVLGRAACLNAAYSLALAD
                     PEDSDVNAIFQAIKIYDELAAAGESVEIALIAGDHMHMLEGDRKIGASIDSLVRETGV
                     DNCIVVTDGAEDEFVIPIVQSRVPVSSIRRVIVSQMPNLEGTYYLIKKILNDPKVARL
                     VLVPIGLLMLFWPIAYLAGRSELAPVIVVGAIGIYLLYRGLGIDDLFRGFATALQTSL
                     TRGRFSFVTYIAGILLVIIGVILGLMNILIYYTEIGILLYVLIFVYGAVLWLALAGIV
                     ASTGIVIDNFFHDRQRLAKVIIFPFFIGAIGLITYGASIYAIAVSNVPDFPFSTANAI
                     TYMLYGTIGGLLCAFAGIGIQYLVNRYMNTSDKTPEVIELV"
     gene            complement(4933..5793)
                     /locus_tag="PK154_05715"
     CDS             complement(4933..5793)
                     /locus_tag="PK154_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HPW10594.1"
                     /translation="MLNELIEKRKKILAESEQHKNHRNELNANASKFARERNTLNNQT
                     REYVEEAQKNKELRDKFNNEVQSLKDQRNVINEQANALFEEIEGFKKDHGGLKNRGLK
                     ELQKQIEHLEFRQQTEVISTDKERELIEKIKQMKETMREQEAELEQNKEVKVKVTSAR
                     DLRRQASEMHAKVTEMAELAQQHHDLMVEFYRKADKSREEADASHRSFVEAQEAADAE
                     HKYFIACQKELRDYDKVISGLRKKTKKAKVSKEQKAVRKEAEHVYKMFRAGEKLTTDD
                     ILLLQRSKLI"
     gene            complement(5787..6077)
                     /locus_tag="PK154_05720"
     CDS             complement(5787..6077)
                     /locus_tag="PK154_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10595.1"
                     /translation="MKGYSGMTVYGDPDMRPFWYRESNMMDRQLNKGSRKIAFAVHAG
                     AQPLIHCRDTSFTIPGMSADEQKVCAVDCIHILQTVPNGDTRPVVVEGGRAC"
     gene            6422..6619
                     /locus_tag="PK154_05725"
     CDS             6422..6619
                     /locus_tag="PK154_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10596.1"
                     /translation="MDLICLVDSGGIVRHKVAECRKVVVGAVSVMCPVPVKKKTGQLD
                     PIPQMYWWYGPAQGGRCAGQL"
     gene            complement(6641..7792)
                     /gene="ftsZ"
                     /locus_tag="PK154_05730"
     CDS             complement(6641..7792)
                     /gene="ftsZ"
                     /locus_tag="PK154_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW10597.1"
                     /translation="MQSIINEALKNAEIEKEQKSPQSVDDDFIGQPRIMIIGCGGAGN
                     NTVNRLHHMGVAGAETLAINTDKQHLDMIQADKRVLIGKSLTRGLGAGGYPDVGKRAA
                     EMARPTLEALLENADLVFITAGMGGGTGTGSAPVVAQIAKEQGAIVVGMVTYPFQVEK
                     ARLIRAEEGLETLASNSDSVIVLDNNRLKNFVPNLPLGQAFSVMDQLMGETVKGISET
                     ITEPSLINIDYADVRAIMSKGGVAVMLVGESKQQNKSESVVRECLSNPMLDVDYRGAT
                     GSLIHITGGSDLTLQDAEEIATSLTYELDPHADVIWGARVRNDMEGKVRVMAIMTGVQ
                     SGKILGNRVSYKSVLEEDARKKEDPKAPQLPRNRKARDFTSGGSLLEWF"
BASE COUNT         1742 a         2157 c         2162 g         1925 t
ORIGIN      
        1 gacggggtgc ttgtcatcag cccttactat aacaagccga accggtccgg gctggtcaag
       61 cactacacga tcctcgcaga cctggacatc ccggtcatcg tgtacaatgt accagggagg
      121 acgggccaga acctcccccc cgatctcgtt gtggaactgg cgaaacatcc caatatcgtc
      181 gggatcaagg aagcctcggg cgatatcggc cagatctcgc gcatcattga gggaaccctt
      241 gatgaggagt tccaggtcat ttccggggac gacaacctga cgttccccat cctggccctg
      301 gggggaagcg gcgtcatctc ggtcgcagca aacgtagagc cgggccggat ggtcgccatg
      361 ttcgaggcgt tctgcgagag tgactttgag accgctctcg acctccatta tgagctctcg
      421 cctctcttcc ggtcgatgtt catcgatacc aacccgatac cggtcaagaa agcagtggaa
      481 ctccgcggca tggcagcggg ttctgtcagg ctgccccttg acgagcttga ccatgagaag
      541 acctcacggc tgcgggaggt cctcgccagc tatgattaag gtggtggtct gcggtgccct
      601 tgggcggatg ggcacaacgg taggccggct cgcgaacgaa gcacccgaca tggaacttgt
      661 cgggggaatc gatctccgcg ggggctcttt ttttggcgta gacgtcaccg aagcagcgca
      721 gattgatacg ctccttgctt cggccagacc ggatgtgctc atcgatttca ctgcgccggc
      781 tgcctctgtc agccatgtca gggctgctgc aaagagcaga accgcggtag tggtggggac
      841 gaccggtatt tcacccgagc aacgggctga gatggaaaaa gcgatcaagg ggaccatccc
      901 tgcggtgatc tcaagcaact tctcaatcgg ggtgaatatc ttctggaacc tgctcaggga
      961 ggctgcccgg atgctgccag aatatgacgt ggaagtgata gaggcacatc accgttacaa
     1021 gaaggatgcc ccgagcggaa cggctaagac cattcttcag atccttgatg aagagctcgg
     1081 tttgcgggag aagaaatatg gccgggaagg gatgatggaa cgcggcaggg agatcggcgt
     1141 ccatgttatc aggggaggcg acattgttgg cgatcactcc gttctcttct ccggtaatta
     1201 cgagaccatc accctctcgc accgggccta tgaccggtcg gtgttcgctc acggggcact
     1261 caaggccgcc cgctgggtgt ccggaaagaa gccaggcatc tatgggatgg cggaagtgct
     1321 tgacctgaag tgaccgttca gaatagctcg aacgaaactt atttcttttg tctggaaaaa
     1381 acgggtaagg tatatcatga cagctgttgt tgacaaggac aagtgcactg cctgtgagac
     1441 ctgtatcgat gaatgccctg ctgccgcaat tagtctgaat gacgggaagg caaaagttga
     1501 caaggacctc tgtgtcgact gtggttcctg cgttgatgtc tgtccgtcag aggccatcac
     1561 cctggaataa aaacacacga tttaattact ccgactgact aactgctttt acttatgatc
     1621 aatgtcggag tgcttggtgc tacaggagca gtcgggcaga ggtttgttca gctcctggct
     1681 gagcatccat ggttcaacct gacgacactg actgcttccg aacggagtgc agggaagaag
     1741 taccgtgatg tcgtcaagtg gcgtcttgat gtaccgttcc cggacaacat cggggatatc
     1801 cgtgtcagtc ccacaaacgt ggcaagtctg aaagaggtgg acctggtctt ctcggccctc
     1861 cctgctgaaa ttgccaagga tgtggagact gcatgcgcaa atggcgggct tgccgtctgc
     1921 agcaatgcga gttcccaccg gatggaaccg gatatacccc tggttgtccc ggaggtaaac
     1981 cccgatcatc tggcgctgat cgatgtgcag cgggatcatg ggcgggacgg attcatcgtc
     2041 accaacccca actgctcaac catcgtgatg acgcttgcac ttgcgcctgt ccatggatgc
     2101 tccttccaga agatccatgt tgcaaccatg caggcggtat cgggggccgg gtttgagggg
     2161 gtccctgcca tggggatcta tgataatgtt atcccctaca tcggccagga agagcagaag
     2221 atggagaccg agaccctcaa gattatgggc acgttcagga acaacgcggt ccagcccgcc
     2281 ccattccagg tcagcgccag ctgccaccgt gtcccggtgc ttgacggcca taccatggcg
     2341 atctggctcg atacccgtga accggtagag aaggtaaaag aggcattcct ccgctacaag
     2401 ccacccatcg agggtctgcc gacacagccg aaggaatcgg tccatttctt cgatgagcct
     2461 gaccggcccc agccccggct ggaccgcaac cgggggaacg ggatgacggt atcggtggga
     2521 cggctccgcg aaggtatccg ttttatcgcc atggggcaca acaccatccg cggggcggca
     2581 ggggcctctg tactgaatgc tgaactgatc tcctccagaa agtatctctg aagtggtatc
     2641 catgctgaaa gacaatacag tattcgtcgg taacaagcca gtcatgaact acgtgcttgc
     2701 agtggtaacc cagttcaaca atggagcctc ggaagtcgcg atcaaggcac ggggaaaagc
     2761 catatcgagg gctgttgata ccgctgagat agccctgaac cggtttctcg aaaacgtgag
     2821 caagaaagag atcatcacct cgactgagat catcgacact gacagtggaa agacaaacgt
     2881 gtcaagcatt gaaattgtcc tggtaaacca gcgataattc tctccctttt ttcctgcgtt
     2941 ccccgtccct ccctggcaag ttcatacaac ctcccggttc agccgctgca ccgggcgggt
     3001 cattcaggta actaccagaa aggatataag acactcaaca acacttagta ttcaatgaaa
     3061 ggctgggctc tcgcagggtg ctttatgctt ctggccgtcc ttaccgtgcc ggtcttcgcg
     3121 accacgaata ccgataagta tgggtacatc acgatagagg cggtcgacat taccctcgac
     3181 ggggggaaag ccaccatcga tatgaagtat acccttgatg aaccgacacg agtaatcgtc
     3241 cttctcctcg gaaaacagga tctgaaaaac agactcctca ccgtcctcaa ttatgaggat
     3301 gccgaggtga aaaatctcga cctcaattcc gctaaactgg tggtggatga catctcttac
     3361 tcgtacgggc ggggggtcta ctggttcccc tctcacacgt tcaatgtcct gatccccacg
     3421 ctccggatca ccaccccgca ggtctcacgg gagtacgctg cagtgactga gatcccgaat
     3481 gggatcggtt attttgcctc ataatctccc tgtcttcttt tgggatgtcc tcgctgtccg
     3541 gataatggaa taacaaaaaa ccggaatcgt ggcaaacacc ttccaggata cccatcaaga
     3601 ttggtccgga atattgtctg ccacttgtgg agttagtacc ctttcatagt ttcgttcttc
     3661 acaactctcc cttcgataca ggacatagaa cggataaatt gagcacgcta tcgtggggaa
     3721 ggaaaaaaat gtccgctctt tcagaccagc tcgatgactt cgggtgtttt atcactcgtg
     3781 ttcatatacc ggttcacgag atactggatc ccgataccgg caaatgcaca gaggagcccg
     3841 ccaatcgtgc cgtacagcat gtaggtgatt gcattggccg tggagaaggg gaaatccggg
     3901 acattgctta ccgctatggc atagatgctc gcgccatagg tgatcaggcc gatcgcaccg
     3961 atgaagaagg ggaatatgat gactttggcg agtctctggc gatcatggaa gaagttgtcg
     4021 atcacaatcc cggtggaagc aacgatgccg gcaagggcca gccagagcac ggctccatag
     4081 acgaagatca gcacatagag gaggatccct atctctgtat aatagatgag gatattcatc
     4141 agtccaagga tcaccccgat tataacaagg aggatcccgg cgatataggt gacaaaggag
     4201 aacctgcccc tggtgagcga ggtctggagg gcagtggcaa atcccctgaa gaggtcatcg
     4261 attccgagac cccggtacag gaggtagatc ccgatcgctc ccacgacaat aaccggtgcc
     4321 agttccgatc tccccgcaag gtatgcgatc ggccagaaca gcatcaagag accgatcggc
     4381 acaaggacca gcctcgccac cttgggatcg ttcaggatct ttttgatcag gtagtaggtc
     4441 ccttcaaggt tcggcatctg gctgacaatg accctccgga tgcttgagac gggaaccctg
     4501 gactggacga tggggataac aaactcatcc tcagccccat cggtaacgac gatacagttg
     4561 tcaaccccgg tctctctcac aaggctgtca atggatgccc cgatcttcct gtccccctcg
     4621 agcatgtgca tgtggtcgcc ggcgatgagg gctatctcca cactctcccc agcggcagct
     4681 agttcatcat agatcttgat cgcctggaat atggcgttga catcagaatc ctcgggatcg
     4741 gcgagtgcga gggaatatgc ggcgttcagg catgctgccc tgccgaggac ggggctctcg
     4801 actcgccctt tatacccgat atcgtcatcg cgatcgacac aaagaaccag tgtccgcccc
     4861 gtctgcatca caatagaaat cgcgatctat ctattaaatt gttacttcgg atgaacgggg
     4921 ttaaaaatga agttaaataa gttttgagcg ctggaggagg aggatatcat cggtcgtgag
     4981 tttctccccg gccctgaaca tcttgtagac gtgttcggcc tctttccgga ccgccttctg
     5041 ctctttcgag actttggctt tcttggtctt cttgcgaagg cctgagatca ccttgtcgta
     5101 atcacgcagt tctttctggc aggcgatgaa gtacttgtgt tctgcatcag ctgcctcctg
     5161 tgcctcgaca aaactgcggt gtgatgcatc ggcttcctcc cgtgacttat cggctttgcg
     5221 gtagaactcg accatcaggt catggtgttg ctgggcaagt tcggccatct cggtcacctt
     5281 tgcatgcatc tcggaggcct gcctgcggag atcgcgggcg gacgtgactt ttaccttgac
     5341 ttctttgttc tgttcaagct cggcctcctg ttcacgcatc gtctctttca tctgcttgat
     5401 cttctcgatc agctcgcgct ccttatcagt gctgatgact tccgtctgct ggcggaattc
     5461 gaggtgctcg atctgcttct ggagctcctt cagcccacgg tttttcagtc ctccgtggtc
     5521 tttcttgaat ccttcgatct cttcaaacag ggcattggcc tgttcattga ttacgtttcg
     5581 ctgatctttt aaggactgga cctcgttgtt gaatttgtcc cgcagctcct tgttcttctg
     5641 cgcttcttca acatattccc tggtctggtt gttcagggtg ttccgttccc gtgcgaattt
     5701 gctggcattc gcattgagct catttctgtg gtttttatgc tgttcagact cggcaagaat
     5761 ctttttacgt ttctcaatca gttcattcaa catgccctac ctccctcgac tacaaccggg
     5821 cgggtatctc cgttaggaac ggtctgcagg atatggatac agtcaactgc gcataccttc
     5881 tgctcatcag cactcatgcc aggtatggta aaagaggtat ccctgcaatg aatgagtggc
     5941 tgtgcgccag catgcaccgc aaatgcgatt ttgcgcgagc ctttgttcaa ttgtctgtcc
     6001 atcatgttcg actcccgata ccagaaggga cgcatgtccg gatctccata aacggtcatt
     6061 ccggaatagc ccttcacaca gcagacggtt ccgcgggtga taatccttca ggatttctat
     6121 atagtacacg ttattccttc atttaataat ttccgaagga aggggccgga gtcgcttgca
     6181 tggggggagg attcttgacg tcagatgagg tgatcccggg tatggctcct atacgttcct
     6241 ccttaacaac agaacatatt cctgacagta tcctgattca tctttgtaga ggaaggcaga
     6301 gagatgggag tgctggttca acaggaattt ttatatgaaa atgcctccgg cattttcatc
     6361 ttttatgcgt gtggcccaca aaggaatcat gttatttttt taaaaacagg tcaaccaggt
     6421 cttggacctg atctgtcttg ttgatagtgg tggcatcgtc cggcacaagg tggcagagtg
     6481 ccggaaggtt gttgtcgggg cggtatcggt tatgtgccct gtcccggtta aaaaaaagac
     6541 cggtcaactg gacccgattc cgcagatgta ttggtggtat ggtccggcac aaggtggcag
     6601 atgtgccggg cagttgtgaa tcaaggtggc gtgttgttgt tcagaaccat tccagtaagc
     6661 tacctccgct cgtgaagtcc cgtgccttgc ggttcctggg gagctggggg gccttcgggt
     6721 cctccttctt tctggcatcc tcctcaagga ccgacttgta agagacacgg tttccaagaa
     6781 tctttccgct ctggactcct gtcatgatgg ccatgacgcg taccttgcct tccatgtcgt
     6841 tcctgacgcg ggcaccccat atgacatcgg cgtgcgggtc aagttcgtag gtaagcgagg
     6901 tggcgatctc ctcggcatcc tgcagggtca ggtcacttcc accagtgatg tggatcaggc
     6961 ttccggttgc tccgcggtag tcgacgtcaa gcatcgggtt gctcaggcat tccctgacaa
     7021 cgctctcaga cttgttctgc tgcttgctct cgcctaccag catcactgca actcctccct
     7081 tgctcatgat cgcacggaca tcggcataat cgatgttgat gagtgacggc tcagtgatag
     7141 tctcggagat ccccttgacc gtctctccca tcagctgatc catcactgag aacgcctggc
     7201 caagcgggag gttgggcaca aagtttttca ggcggttgtt gtcgagcaca atgaccgagt
     7261 cggagtttga agcgagcgtc tccagcccct cttctgcgcg gatcagtctg gctttctcga
     7321 cctggaaggg ataggtgacc atcccgacca cgatggcacc ctgctctttt gcgatctgtg
     7381 caaccaccgg ggccgaacct gtaccggtcc ctccccccat gcctgccgtg atgaagacga
     7441 gatcggcgtt ctccaaaaga gcctcaaggg tagggcgtgc catttcagca gcacgtttcc
     7501 cgacatcggg atagcccccg gcaccaagtc cccgtgtcag ggatttgccg atcagcactc
     7561 tcttgtctgc ctggatcatg tcgaggtgtt gcttgtcggt gttgatggca agtgtctccg
     7621 cgccggcaac acccatgtgg tggagacggt tcacggtatt gttcccggct ccaccgcagc
     7681 cgatgatcat gattcgcggc tgtccgatga agtcatcatc cacactctgt ggacttttct
     7741 gttccttctc aatttctgcg tttttgagcg cttcattgat gatactctgc atttctgacc
     7801 ctcctaaacc ttgaatgaaa cctggtcact ctctttgagg acacgcccgc cttgcctttc
     7861 gctcaggctt cccgaccatc agatcgagat gcttcagcaa atggtggaag cgggggagtt
     7921 tccgaccatc tctgaagcat ctcgatctga tggtcgggaa gcctgagcgt aattcgctgc
     7981 attttt
//
LOCUS       DAOOCO010000080         7915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8643_length_7915_cov_4.936005, whole genome shotgun sequence.
ACCESSION   DAOOCO010000080 DAOOCO010000000
VERSION     DAOOCO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8643_length_7915_cov_4.936005"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="PK154_05735"
     CDS             complement(<1..1627)
                     /locus_tag="PK154_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW10598.1"
                     /translation="MPAGDGPLVSLTWQPVPGAPGALMYPLIRKIDVISSNSYVITTP
                     EVILIVDPGGVPEQGEQLSRLIEACREECDRPVFAFLTHSHVDHFIGIQSNAAFSVQK
                     AVVFAVHHAGALALECGDRTVTQADILQQPLSPMEVGLRLFAPERENHAGVPASLCFP
                     NGAEITIVQDPAKADSPRLPHEQIQFGNGPALDLYHTPGHSPDSICLRIGNLLFVGDL
                     LFAANPGVAGLTGWSQESLVRSLSGAERLVAEGGVTHVCPGHGNIIPAGDAARMFSRV
                     RADAQSLSHIAELNCDRAVATAEYATDCMEQVNELFTIMAGRLAYVTYILDELGEAGM
                     AEETAALISTDAIDELLEAFRDFAGEYQKISPSPVPLMLKAAQVMGKLERTFQRNELS
                     GIIDPTLVRRAARLLADYITMLRGFTLPGERQVLDIVPVIEAIVTGLSLPLLSDEEIL
                     AGPDDEGAFSRILLSRIGSRPLLDEVECEVVAEDSPLLAWIDRDHFTDLFTYILEDLV
                     GTGAGRIGIRTRKGEEGATITITGILAVTGAREP"
     gene            complement(1614..1946)
                     /locus_tag="PK154_05740"
     CDS             complement(1614..1946)
                     /locus_tag="PK154_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPW10599.1"
                     /translation="MEIRTAHREGGTVLSVNGRIDTTAAPELDQAILQEIDQGNRKIL
                     LDFSGVPYISSGGLRVILATAKKLKTPGDKFGLCSLSPEVFKIMKLAGFTSIFSIYPD
                     EGEALARW"
     gene            2040..2270
                     /locus_tag="PK154_05745"
     CDS             2040..2270
                     /locus_tag="PK154_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10600.1"
                     /translation="MSNPRATQLYECLIMESPIRSSLLVLLQISWPKEVPLTSHTLPA
                     EEGIQAMPETVRIFFGDIPRFDDITSMMIGSR"
     gene            2273..2689
                     /locus_tag="PK154_05750"
     CDS             2273..2689
                     /locus_tag="PK154_05750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW10601.1"
                     /translation="METRFDLAIGSEVERVRAVTDRLEEAMQLHGFSSEEILDTQLAV
                     EEAITNVIVHGYGRPGESILVSSRIRSDRAEVCITDTAPRFDPLSVADPDIGEGIDDR
                     EIGGLGVFLIRRVMDEVSYRYEGGKNVLVLTKVRRS"
     gene            2781..3239
                     /locus_tag="PK154_05755"
     CDS             2781..3239
                     /locus_tag="PK154_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10602.1"
                     /translation="MKILIVYMSYHHMNTEKIARAMAEVTGATLSKADEVDPGDLAGY
                     DLIGFGSGIYGGKHHTSILALADEMPPMEKPVFLFSTSGGYKEEFHTPLRRNVITKGC
                     TIINEFHCPGEATFLKFIRVNKGRPDEQDLENARTFAKGLLAAPGAEKQE"
     gene            3285..3806
                     /locus_tag="PK154_05760"
     CDS             3285..3806
                     /locus_tag="PK154_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10603.1"
                     /translation="MSSRKRKQEYVRTTGDRSREELIFATLSLPIATDTRVAGLVRVL
                     NDLPDVYTCSSCGGHSDLRDRENPVPEGYFYIQFILEPTESGFFSLGIIDLAARNVDN
                     ENLAVRVLNCTDNPKLVMFHILGKEGVDPGEFAEEIRSLCKEWGVSCEGVYERKKKVR
                     RQIAYDRIQKGDS"
     gene            3911..4669
                     /locus_tag="PK154_05765"
     CDS             3911..4669
                     /locus_tag="PK154_05765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW10604.1"
                     /translation="MNNTNQDTAAGDLLQEIRSILDQGPDLPGTERILSLVGCPHVSE
                     SLLEGLRIYSDYLPKATEHPFSPGKRYLHFLWDAFDRALLSLSMPIAFPFRRMIAERL
                     FSRCGKNFNAEGDIRFNFGQLLAVGDDVFLNRGSFIDTKGGVMIGNAVGIGEFVRIFT
                     HAHSESIHSVRTYSPVTIQDYAKVFSGAMILPGVTIGRQAIVAGGAVVTTDVPENSVV
                     AGVPARVIRERRTEGRTGADLDHVWLHKGAFQDE"
     gene            4831..5328
                     /locus_tag="PK154_05770"
     CDS             4831..5328
                     /locus_tag="PK154_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10605.1"
                     /translation="MRVIGQDTPDTVGKPREKEPVCKSLSYESKAQISRYQDIFSIDE
                     RVRELVLRINALPDICTMTSCGGHPIPDEEKGQLPDGEFNVGFFIKPTIKGLRSLGIV
                     DQAASNLDDQNIMIRVWNDSNNPNFVVFILTGRNCVEPSFLAEEIYTLGKIWIDGDWT
                     ARKFF"
     gene            5344..6357
                     /locus_tag="PK154_05775"
     CDS             5344..6357
                     /locus_tag="PK154_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10606.1"
                     /translation="MQPLRCSHCRSLSRTIFMLLSLLLFISLAGCLSSPSGGISPSGT
                     LSPPGTNVPDNPLATTNDSVSSQVIQKHYSTFDSISLTVNNITVKSIPKRSGDTFSVV
                     VLNLSVKNGGLDESFLFENRSLVSFQSDSEHLFPEYPQFEYSSVPITNPLINRSAAPG
                     EEIQGDVYFFLNPGVHSMSLSIRYPDWTIVGGAYLPDLSNANRALSDNEYPKYLALEV
                     PSAVRKPTIPGWRASPGHGVAIINVSITNHNHERAVIRSEHLWLLTEKPITLEHGGDK
                     MTPEMARDYLRFPIVVPAGVTTNGSVFFGVYSGTRINKIALADENLVIHSIVDLNSIY
                     QYE"
     gene            6468..7748
                     /locus_tag="PK154_05780"
     CDS             6468..7748
                     /locus_tag="PK154_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10607.1"
                     /translation="MDFRHFRFVMVILIVVLTIGVGFVTAAGMGRGAVQEVRLDPDPS
                     DTDTEYGRSVAIDGNLVAVGMGGDGAIGSVYVYRHMGPTYVLDEKLAFPEEVSCDCLD
                     AKETEDPEDEFTLCPEFGRTVAIQGDTIFVGARFAPVGEISAGAVYVFKNKGDSWVYT
                     AKIVSPNPEAEDNFGRALAVQGNLLVVTARKEDQTSTDVGGAYVYQYTGREWMYYTKL
                     TASDATSSAYFGQSVAIQGEYLVIGARNADPQGAGGFYLFLRDGAEWVETAKVSPPNG
                     KNNDQYGFCIAMSGDTVAVGARRTDQNSIMKDTGAAFVYTIKDGFPVLVTKLTASDAE
                     ASDEFGQSVAFVDDLIAVSAWKDDGKKGSIYLFRMTDGAWTEIGKIQASDGKSGDEFG
                     YSLAALGNRLVTGAHKADFMYKDAGAAYVIPVRL"
BASE COUNT         1802 a         2055 c         2257 g         1801 t
ORIGIN      
        1 ggggttcccg ggctcctgtg acggcgagga ttccggttat ggtgatggtt gccccctctt
       61 ctcccttccg ggttcggatc ccaatcctgc ccgcccctgt cccgacaagg tcttcgagga
      121 tatatgtgaa caggtcagtg aaatggtcac ggtcaatcca cgcaaggaga ggggaatctt
      181 ccgcgacaac ctcgcattcc acctcatcga gcaggggccg tgatccgatt cgggagagca
      241 ggatgcggga gaatgccccc tcgtcgtcag gaccagcgag gatctcttca tcggagagga
      301 gcggtagcga gagaccggtg acgatggcct cgatgaccgg gacgatatca agtacctgcc
      361 tctcacctgg cagcgtgaag ccgcggagca tggtgatgta atcggcaagg agtcgtgccg
      421 ccctccggac gagggtcggg tcgatgatgc cggaaagttc gttccgctgg aaggtccgct
      481 cgagcttccc catcacctgg gcagctttca gcatcagtgg tactgggctc ggggagatct
      541 tctggtactc cccggcaaag tcccggaacg cctccagcag ttcatcgatc gcatcagttg
      601 agatgagcgc agcagtctct tcagccatac cagcctcgcc cagttcatca aggatatacg
      661 tgacataggc caggcgtccg gccatgatcg tgaagagctc gtttacctgc tccatgcaat
      721 cggtggcata ctccgcggtt gccactgccc gatcgcagtt cagctccgcg atatgggaga
      781 gggattgtgc atcagccctg acacgggaga acatccgtgc ggcatctccg gcagggatga
      841 tgttcccgtg ccctgggcag acatgggtaa cccccccttc tgcgaccagc cgttcggccc
      901 cggaaagtga tcgcacgagc gattcctggg accacccggt caggccggca acgcccggat
      961 tggcggcaaa gaggaggtcg ccaacaaaaa ggagattccc gatccgaaga cagatgctgt
     1021 cagggctgtg gcccggggta tggtagagat cgagagcggg tccgttccca aactgtatct
     1081 gttcatgcgg gagtcgggga gagtcagcct tggcaggatc ctgcacaatg gtgatctcgg
     1141 caccgtttgg aaaacagagc gatgcgggaa caccggcatg gttctcccgt tcaggggcaa
     1201 agaggcgaag gcctacctcc atgggggaga ggggctgttg aaggatatcg gcctgcgtga
     1261 ccgtccgatc accgcattcg agcgcgagtg caccagcgtg atgtaccgcg aaaacgacgg
     1321 ctttctgaac ggaaaaggct gcgtttgatt gtatcccgat gaagtgatcg acatgggagt
     1381 gtgtcaggaa cgcgaagacg gggcgatcgc actcctcccg gcaggcctca ataagccggg
     1441 agagttgttc cccctgttcg ggtacacctc cgggatccac gatgaggatc acctcagggg
     1501 tggtgatgac gtacgagttg gatgatatga cgtcgatctt cctgatgagg ggatacatca
     1561 gggcacccgg ggcaccgggt actggctgcc acgtgaggga taccagcgga ccgtcaccag
     1621 cgggcaagcg cctctccctc gtcagggtag atggagaaga tggaggtgaa cccggcgagt
     1681 ttcatgatct tgaatacttc cggggagagg ctgcagaggc cgaacttgtc ccccggggtc
     1741 ttcagtttct tggcggtcgc caggatcacc cgaagccctc cgctgctgat atacgggacg
     1801 ccggaaaagt cgagcaggat cttccggttc ccctggtcga tctcctgcag gatcgcttgg
     1861 tcgagttccg gtgccgcagt ggtatcaatc ctcccattga cggagaggac cgtcccgcct
     1921 tccctgtgtg cagttctgat ttccaccgtt attcccttga gggtatgtcg gtattttcgg
     1981 gagataaccc tatccttcct gttttgccct cgtcatggga tcagagaacc tgacccttta
     2041 tgagcaatcc gcgtgctact caattgtacg agtgcctgat catggagtcc ccgatccgtt
     2101 catcgctgct ggtcctcctg cagatctcat ggccaaaaga ggtccccctc acctcgcata
     2161 ccctgccagc agaggagggt atccaggcga tgcccgaaac ggtcaggatc ttttttgggg
     2221 acattccccg atttgatgat atcacgagca tgatgatagg gagccggtga ttatggagac
     2281 acggtttgac ctggcgatcg gatcggaggt tgaaagggtc cgtgccgtca ccgaccggct
     2341 tgaagaagcg atgcagttgc acgggttctc ctccgaggag atcctcgata cccagcttgc
     2401 tgttgaagaa gcgatcacga acgtgatcgt ccacgggtac ggaagacccg gagagtcgat
     2461 ccttgtctcc tcccggatca ggagcgatcg ggcggaggtg tgcatcaccg atacggcacc
     2521 ccgcttcgat ccgctctcgg tcgctgatcc cgatatcggg gagggtatcg atgaccggga
     2581 gatagggggg ctgggggtat tcctgatccg gagggtcatg gacgaggtct cctaccgcta
     2641 tgagggagga aagaatgttc tggtcctcac gaaggtgaga aggagttgaa ggattccggc
     2701 tccctgagga aggagccttc gcagggatcg ccggatttct catcaggttt atattcccct
     2761 ttccccaaaa tgttcccttc atgaagatcc tgatcgtcta catgtcatac catcatatga
     2821 ataccgagaa gatagccagg gcgatggcgg aagtcaccgg cgcaaccctg tcgaaggccg
     2881 acgaggtcga tcccggggat cttgccgggt atgacctgat cggattcgga tccggtatct
     2941 atgggggcaa acaccacacg tccattcttg cccttgccga tgagatgccc cccatggaga
     3001 aaccggtctt cctcttctcg acatcgggag ggtacaagga ggagttccat actcccctca
     3061 ggaggaacgt gatcacgaag ggatgtacga tcatcaacga gttccattgc cctggtgagg
     3121 caaccttcct caaatttatc cgggtgaaca agggccgccc cgatgagcag gaccttgaga
     3181 atgcacggac ctttgccaaa ggactccttg ctgcccctgg tgcggagaag caggaatgat
     3241 cagaggctgc cccatcggtt gtcatacaat gcccggtcag gtccatgtca tcccgtaaac
     3301 gaaaacagga gtatgtcagg accaccgggg accgatcacg ggaggagctg atctttgcca
     3361 cgctctccct tccgatcgcg accgatacac gggtagcagg gctggtcagg gtgttgaacg
     3421 atctccctga tgtctatacc tgtagttcct gtggtggaca ttccgacctc cgggaccggg
     3481 agaacccggt gcccgaggga tatttctata tccagttcat cctcgagccg acagagagcg
     3541 gctttttctc cctcgggatc atcgaccttg ccgcccggaa tgtcgacaac gagaatctcg
     3601 ccgtgagggt cctgaactgc accgacaacc cgaagctcgt catgttccac atcctcggga
     3661 aggagggggt ggatccgggt gagtttgcag aggagatccg ttcactctgc aaggaatggg
     3721 gtgtctcctg tgagggcgtc tatgaacgga aaaaaaaggt gagacggcag atcgcatatg
     3781 atcggataca gaaaggggat tcgtagaggg ataagggagc agcttctatg ccagaacagc
     3841 ctggtagagg agatggcatg atgactgacc cccgctcaaa gaggattctt cagaaggggg
     3901 aggtgtatcg atgaacaata cgaaccagga tactgctgcc ggggatctcc tccaggaaat
     3961 tcgatcaatc cttgaccagg gacccgacct tcctggaacg gaacggatcc tgtccttagt
     4021 cggatgtccg catgtttcgg aatcgcttct tgaaggtctc cgcatctaca gtgattacct
     4081 gccaaaagcg acggaacatc cgttcagccc tggtaagaga tacctgcatt ttctgtggga
     4141 tgcttttgat cgcgctctcc tctccctctc tatgccgata gcatttccct tccggagaat
     4201 gatagcagag cggttgttct cccgctgcgg gaagaacttc aacgccgaag gggatatcag
     4261 gttcaacttt gggcagctcc tggctgttgg tgacgatgtc ttcctgaacc ggggatcgtt
     4321 catcgatacc aaaggtgggg tcatgatcgg gaacgctgtc gggatcggtg agttcgtcag
     4381 gatctttacc catgcccatt cggaatccat ccattctgtc aggacctatt cgccggtgac
     4441 gatccaggat tatgcgaagg tcttttccgg ggcgatgatc ctgcccggag tcacgatcgg
     4501 ccgccaggcc atcgtagcag gaggagcggt ggtcaccacc gatgtccccg agaattcagt
     4561 ggttgccggt gtcccggccc gggtgatcag ggaacggaga acagagggaa gaaccggagc
     4621 ggatctcgac catgtctggc tgcacaaggg ggcattccag gacgaatgaa aggccgaatc
     4681 tcccctgtcc ctttccggaa aggatcaggg gacctgggac cggccgcaat caaagccagc
     4741 attacccgcc tgagtcgtac aggtcatcct gacccttcgt tctcaatccg gaattatttt
     4801 ataaacttca gtcgcaggta cgaccaaggg atgagagtga ttggtcagga tacaccagat
     4861 actgtaggaa aaccccggga gaaagagccg gtatgcaaat ccctctccta cgaaagtaaa
     4921 gcccagatct cccggtatca ggatattttt tccattgacg aacgggtgag ggagctcgtt
     4981 ttacggatca atgccctccc tgatatctgc accatgacct cctgcggtgg acatccgatc
     5041 cctgatgaag agaagggaca actgcctgat ggtgaattca atgtcggttt ttttatcaag
     5101 ccgactatca aggggctcag gtcactcggg atcgtcgatc aggctgccag caatctcgat
     5161 gatcaaaaca ttatgatcag ggtctggaat gactcgaata atcccaattt tgtggtattc
     5221 atcctgacgg ggagaaactg cgtggagcct tctttcctgg ctgaggagat ctataccctc
     5281 gggaagatct ggatcgatgg ggactggact gcacgaaagt tcttctaacg aggggaagaa
     5341 ttcatgcaac cgctcagatg ttctcactgc cggtcccttt ccaggacaat tttcatgctc
     5401 ttgtcacttc tcctcttcat cagccttgcc gggtgtctct catctccctc aggaggaatt
     5461 tctccatcag gaacactttc tcctccagga acaaatgtgc ctgacaaccc ccttgccaca
     5521 acgaatgatt cagtcagcag tcaggtcatt cagaagcatt actccacctt tgattccatc
     5581 tctctcaccg tcaacaacat cactgtgaaa tctatcccga aaagatcggg tgataccttc
     5641 tccgtggtgg tgctgaacct gtcggtgaaa aacgggggcc tggatgaatc ctttcttttt
     5701 gagaacagat ctctagtctc attccaatcg gattctgaac atctctttcc ggagtatccg
     5761 caatttgagt actcttcagt acccattacc aatcctctca tcaaccggtc tgcagctccc
     5821 ggtgaggaga tacagggtga tgtgtatttc ttcctgaatc ccggggtcca ttcgatgtcc
     5881 ctctctatca gatacccgga ctggaccatt gtcgggggag catatcttcc tgatctctcc
     5941 aacgcaaacc gggcgttgtc tgacaacgaa tacccaaaat atctcgcact tgaggttcca
     6001 tctgcagtcc ggaaacctac catccccggc tggcgggcat ctccaggaca tggggtagca
     6061 atcatcaatg tcagtatcac gaaccataat catgaaagag ccgttatccg gtcggagcat
     6121 ttgtggttgc tgactgaaaa accgataacc ctggagcatg gaggtgacaa gatgacacca
     6181 gagatggccc gggattatct ccgttttccg attgttgtcc ctgcaggagt gacgacgaat
     6241 ggctctgtat tctttggggt ttatagtgga acgcggatca acaagatcgc actcgctgac
     6301 gaaaatctcg taatccactc catagtcgac ctaaacagta tctaccagta cgagtaatac
     6361 ggaatcacca ggttgaggtt aattgaatgt ggaaaactca agagtgcaac aaagatattt
     6421 tataacagct agagagatcc cctttgatcc tgaggaggat tgagaccatg gatttcagac
     6481 atttcaggtt cgtaatggta attctgattg tcgttttgac aatcggcgtt ggatttgtga
     6541 ccgctgctgg aatgggcagg ggggcggtgc aggaagtgcg actggatccc gatccatccg
     6601 atactgatac tgagtatggc cgatccgtgg cgattgacgg caatctcgtc gcagtaggga
     6661 tgggtggaga tggcgcgata ggatctgttt acgtttacag gcacatgggt ccaacctatg
     6721 tccttgatga gaaactggct tttcctgaag aggtatcatg cgattgtctc gacgctaagg
     6781 aaactgaaga tcctgaagac gaatttaccc tgtgtccgga atttgggaga acggtagcta
     6841 tccaggggga cacgattttc gtgggagcac gctttgcacc cgttggtgaa ataagcgctg
     6901 gggcagtcta tgtgtttaaa aataaagggg attcatgggt atacactgcc aagattgtct
     6961 ccccaaatcc tgaagcagag gataattttg ggcgcgctct cgcggttcag gggaacctcc
     7021 tcgtggtgac tgcgagaaaa gaggatcaga ccagcaccga tgtaggtggg gcatatgtgt
     7081 atcagtatac gggtagggag tggatgtact acacaaaact caccgcaagt gatgcaactt
     7141 cgagtgctta ttttggtcaa tcagttgcga ttcagggaga gtacctggtc atcggggccc
     7201 gcaatgctga tccacaaggc gccggtgggt tttacctctt tcttagagac ggggctgaat
     7261 gggttgagac cgccaaggta agcccgccca acgggaagaa taacgatcag tatggcttct
     7321 gcattgcaat gtccggagat accgtggcag tgggtgcccg caggacggat cagaatagta
     7381 tcatgaagga caccggcgca gcctttgtct atacaatcaa agatggtttc ccagtactgg
     7441 tcaccaagct taccgccagt gatgcagagg cgtcggatga gtttggccag tcggttgcgt
     7501 tcgtggatga tctcattgcg gtaagtgcct ggaaagatga cgggaaaaag gggtccatct
     7561 atctcttccg aatgactgat ggtgcatgga ctgagatagg taagatccag gcatccgatg
     7621 gcaagagtgg agacgaattc gggtattccc ttgcagccct tggcaacagg ttggtcaccg
     7681 gtgctcacaa ggctgatttc atgtacaaag atgccggggc agcatacgtg atccctgtaa
     7741 ggctatagcc agaaaaatca ctatcttact tccctttttc aaagcaggcg agacaagcct
     7801 ctgatgggta atgatcccct tcttttacag tttcggattt tcgatattcc cttaatgatt
     7861 gttatcccct ttgaagcgaa ccgacatcca tgacccttcc ccgttggcag tgcct
//
LOCUS       DAOOCO010000081         7863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8719_length_7863_cov_5.214780, whole genome shotgun sequence.
ACCESSION   DAOOCO010000081 DAOOCO010000000
VERSION     DAOOCO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_7863_cov_5.214780"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1138
                     /locus_tag="PK154_05785"
     CDS             242..1138
                     /locus_tag="PK154_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW10608.1"
                     /translation="MIRGLRAIGEESVIIDARVRPPFKSLTQVLASEPGARPPVKRSP
                     LVSGYERTPSMTQKSWELFLDEMDAAGVDKGILVGRQAGHRTILNDDIAELRDRYPER
                     FPIALAGINGADVDAGVREIERTLGELRFKGIAVDPGWWVPARYVDDPKNYPLYEKCD
                     ELGGVLYLTCSLMQGLDISYAVFCLKKKNANAFPTLQIVVVHAAFPYTSEMIGVMIAN
                     APLGNLWVIPDFFQFIPGFPGAREWVDAANHFLGDRILYASSYPVRPLAQSLAEFQRF
                     SYQPDVLESILSGNAARLFGMK"
     gene            1160..2134
                     /locus_tag="PK154_05790"
     CDS             1160..2134
                     /locus_tag="PK154_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10609.1"
                     /translation="MDETIKAELLSVGTIAIEESLLPPPRTSTAGPGMGLQNVFFSSG
                     GLRVRLEVNRDSPLKMTRKDDHVVISRDNTELAEGIIEEVVAHCPGQVYITVSERCIY
                     NCQFCSVPLSKGKIKTKEEILAIVERAREKGTLKVIALTSGIATSPDDEIDRVADIVK
                     SLVPYGVPIGVAVHPAKDSSRKLKEAGVTEVKYSVETMDPEIFDRICRGRKGHNLKFI
                     LESLREAVEVFGRNHVSTNIIIGLGETDECVRHGVEYLAQMGVIAVLRPISISPLRKE
                     ALEHAVRPSPERLLKLTTMTREILRKYGLSTRESQTMCLACTGCDLTP"
     gene            complement(2190..2657)
                     /locus_tag="PK154_05795"
     CDS             complement(2190..2657)
                     /locus_tag="PK154_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10610.1"
                     /translation="MEEECCPRFDPAPWDGKVLEWENKRFVRDRVRTFFFIPLNFGGV
                     ITRLNGKVEKAGATIPDNLCLSDDTSRWNMDIYLAVDREVPDVENVTLSGKFLCRVYE
                     GPYGDTGKWCRDFESYARERYLSIKKWYMWYTTCPKCARKYGKNYVAVLGQVG"
     gene            complement(2737..2994)
                     /locus_tag="PK154_05800"
     CDS             complement(2737..2994)
                     /locus_tag="PK154_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10611.1"
                     /translation="MYLFSMDASQGYLAISITILAIVALLLFIAGRKKPREDLSGLVA
                     ISLALITAGIVFGENLLVGYSLIGAGVLLAVIDIYLTMTHQ"
     gene            complement(3176..4351)
                     /locus_tag="PK154_05805"
     CDS             complement(3176..4351)
                     /locus_tag="PK154_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10612.1"
                     /translation="MTKHDPISLVHGIMQEFARMTGLDPPSPSPRRYLWTDAFAVCNY
                     LELYHRTNDAAYRDLALRLVDQVHHVLGRHREDDPRKGWISGLHEQQGELHPTIGGLR
                     IGKKLNERMSGEPFDERLEWDRDGQYYHYLTKWMHALNRVSRMYGDPQYTRWALELAK
                     TAHARFTYLPASGGRKRMFWKMSVDLSHPQVSSMGLHDPIDGLVTYRDLQMTLESFQQ
                     PSPKVNLFTEIAEMEEMCRMQDWATDDPLGIGGLLFDASRIAQLMVQGGFAHPDLLES
                     VTESALQGMRTVKRSRLLQLPAGYRLAFRELGLSIGMKGLEELSGMIEGYPAVFGTKD
                     SLHRKVAELRKYMPLAEEIELFWLDEDNQGAGTWTEHQEINMVMLATSLVPDGFLMI"
     gene            complement(4425..4703)
                     /locus_tag="PK154_05810"
     CDS             complement(4425..4703)
                     /locus_tag="PK154_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW10613.1"
                     /translation="MSIIRSIFPSHCYLFFIGVDPRLHGKGYGGKLIRPMLDWMDRKQ
                     IACYLNTQNEKNIGLYEHFGFTVVEQVSLPGSGILHTGMVRPPVVQGE"
     gene            complement(4645..5055)
                     /locus_tag="PK154_05815"
     CDS             complement(4645..5055)
                     /locus_tag="PK154_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10614.1"
                     /translation="MVEQPGAIIRLSKEQIGPASEMLVEAFFNDAKLTYVLPDDTTRR
                     EEGRNIFEFHLRYGLNYGRVYATSPTLEGVAVWLPSNRSEVTFWRAMRSGGMALQKGL
                     SKDAMKRLLAFAEEVDVYHKKHLPIPLLPLLHRC"
     gene            5154..5831
                     /locus_tag="PK154_05820"
     CDS             5154..5831
                     /locus_tag="PK154_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPW10615.1"
                     /translation="MKFSQEVLDAFPGIGVAEGNIRSVQIAHESAGLETLKRETIQEV
                     KSRYTLEQVKDDPVFRAYRDFFWSVGVDPTKTRPASEALVRRILSGGKLPRINTAVDS
                     YNLASARSGVPIAAFDSDALTGDLTLRFANNGESFLGIGMEKQVVLQKNQVIMTDEKQ
                     IVAIYPYRDSDTTKVTAETRNIRIITCGVPGIGRELVSGAFDLCAKYLEKYTGGMSKG
                     AEIFPKK"
     gene            complement(5938..6540)
                     /locus_tag="PK154_05825"
     CDS             complement(5938..6540)
                     /locus_tag="PK154_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10616.1"
                     /translation="MVPDSPCPGKQGACSENHHLTSPLQTAVVIRIPLAETDSRTLHN
                     HLYAIYRRDNSMNTLIIYKSYSKMNTEKVAKAMAEAMNATLKKVEEAKPEDLTGYDLI
                     GIGSGIYAGKYHKDLYKLLEKIPRLEKDMFVFSTAGSPDEKYEKPMKELLAGKGARVV
                     GEFRCPGAAGLLGLTWANKGHPDEQDLERARTFAKGLIKV"
     gene            6666..7004
                     /locus_tag="PK154_05830"
     CDS             6666..7004
                     /locus_tag="PK154_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10617.1"
                     /translation="MQKGTEKAPPPNKVKRSGNTREQPGDGSTNRTGLLPTPGRMEFS
                     DRTTIQISKDTKRDLDRIKRESGGKTYDDAIRFLLDERRRLRPSTFGLLSGAGPFVRD
                     EEEDSHRIRP"
     gene            7045..7362
                     /locus_tag="PK154_05835"
     CDS             7045..7362
                     /locus_tag="PK154_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPW10618.1"
                     /translation="MRPWVEEQGPLFSSTITFAETVRYFTIQGIDPATLLHCLDDIRT
                     RSTVLPLDEQLAIFAGHLKNREVEGIADAIVLATARAGGHQVVTGDPHFRDVEDAVYL
                     GEE"
     gene            7404..7625
                     /locus_tag="PK154_05840"
     CDS             7404..7625
                     /locus_tag="PK154_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10619.1"
                     /translation="MRMSGRVRPPRQNDSPHQVSHQASGGSREGGPADTPGIAGELAE
                     KKGMDKTTLLEHIHKAEKRLIGHILAQGA"
     gene            complement(7646..>7863)
                     /locus_tag="PK154_05845"
     CDS             complement(7646..>7863)
                     /locus_tag="PK154_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW10620.1"
                     /translation="IRVPAVFGIAAALPVIIISLVLVSGVNRVSGMMTAVQKNEKWIK
                     WAVAAVFIIVGVYYIVIVYGPMVGIGI"
BASE COUNT         1781 a         2169 c         2138 g         1775 t
ORIGIN      
        1 aggtatgaaa tcatgtgatg gcgtgaaaaa atggtggatg aaatgccact ataccctttt
       61 ttatatgaaa atgcctccgg cattttcatc ttttatgctg tggccctcga aggaatcatg
      121 ttattttttt atatctggtg aaggtcgtcc gatcttctga aacatcgagg ggtatccttc
      181 tcgggtgaac agatcccggg cactaccaga tgcatggagt tatcatccga caggaaagaa
      241 agtgatcaga ggcctccggg ccattggaga ggagagcgtg atcatcgatg cacgggtacg
      301 accccctttt aaaagcctga cccaggtcct ggcatcggaa cctggcgccc ggcctccggt
      361 aaagcgaagt ccgttggtaa gcgggtatga acgcacccct tccatgacgc agaaatcatg
      421 ggaactgttc ctggatgaga tggatgcagc aggggttgac aaggggatac tcgtcggccg
      481 gcaggcaggg caccggacga tcctgaacga cgatatcgcc gagctccgcg accggtatcc
      541 cgaacggttc ccgatcgccc ttgcgggcat caacggggct gatgtcgatg caggggtcag
      601 ggagatcgaa cggacgctcg gggagctgcg ttttaaaggg atcgcggtcg atcccgggtg
      661 gtgggttcca gcccggtatg ttgacgaccc gaagaattat cctctctacg agaaatgtga
      721 tgaactcggt ggagtactct acctcacctg cagcctgatg caggggctgg atatctcgta
      781 tgccgtcttc tgcttgaaaa aaaaaaatgc aaacgcgttt cctaccctcc agattgtcgt
      841 ggtccatgcc gcattccctt acacgagcga gatgatcggc gtcatgattg ccaatgctcc
      901 gctggggaac ctctgggtca tcccggactt cttccagttc atccccggat ttcccggtgc
      961 ccgggaatgg gtcgatgcag ccaaccattt cctcggggac cgaatcctct atgcctcctc
     1021 gtacccggtc cggcccctcg cccagagcct tgcagagttc cagcggttct cttaccagcc
     1081 ggatgtgctc gaaagtattc tctccggcaa tgcagcacgt ctgttcggta tgaagtgagt
     1141 gggtgacagg atgatcctca tggacgagac cataaaagca gagctgctct cggtcgggac
     1201 catcgctatc gaagagtcgc tgctgccacc ccccaggaca tcgacggctg ggccggggat
     1261 gggactccag aatgtattct tctcgtccgg aggtcttcgc gtccggctgg aggtgaaccg
     1321 ggactctccc ctgaagatga cccggaaaga cgatcatgtc gtgatctccc gggacaatac
     1381 ggagctggcg gaagggatta tcgaggaagt ggtcgctcac tgccccggcc aggtctacat
     1441 caccgtcagt gaacgctgca tctacaactg ccagttctgc tcggtccccc tctccaaggg
     1501 gaagataaag acaaaggagg agatcctggc catcgttgag agagccaggg agaagggcac
     1561 cctgaaggtc atcgccctga cctctggtat cgcaacatca ccggatgacg agattgatcg
     1621 cgtggcagat attgtcaaat cgctcgtgcc ttacggggtc cccatcggag tcgcggtcca
     1681 ccctgctaaa gattcctcca ggaagctgaa ggaggcgggg gtgaccgaag tgaaatacag
     1741 cgtcgagacc atggatccgg agatcttcga ccgtatctgc agggggagga aggggcacaa
     1801 tctcaagttt atcctggagt cgttgcgtga ggcagtcgag gtcttcggca gaaaccacgt
     1861 ctcgacgaat atcattatcg gccttgggga gacggacgaa tgcgtccggc atggggtgga
     1921 gtatctcgca cagatggggg ttattgccgt cctccgcccg atctccatct cacctttgag
     1981 aaaagaagca cttgaacatg cggtccggcc gagccccgaa cggcttctga agctcaccac
     2041 catgacacgg gagatcctca ggaagtacgg gctctcaacc agggagtcac agaccatgtg
     2101 cctggcatgt accggctgtg accttacacc ctgataccgt gttgagctgg tggcgtaagg
     2161 atacgatacc ttccgggtga gatgcgccgt cagcctacct ggccaagaac tgccacgtag
     2221 ttcttcccgt attttctggc acatttaggg caagtggtgt accacatgta ccatttcttg
     2281 atggaaaggt accgctccct ggcatacgac tcgaagtccc tgcaccattt tcccgtatcg
     2341 ccataaggac cttcatacac cctgcagaga aatttcccgc tcagggtcac gttctccacg
     2401 tccgggacct ccctgtcgac cgccaggtag atgtccatgt tccaccgcga ggtgtcatca
     2461 gagaggcaga gattgtcggg aattgtcgcc cctgccttct ccaccttccc gttgagcctc
     2521 gtgatgacac ccccgaagtt gagggggatg aagaagaacg ttctcaccct gtccctgaca
     2581 aaccgcttgt tctcccattc cagcaccttc ccgtcccagg gagcgggatc gaaccgcggg
     2641 caacattcct cttccatgcc ttcatcccct gataacacag tctcctctgc atgctagaat
     2701 ggaagtatca ccgataaatg ttgtcctgga cacgcttcat tggtgtgtca ttgtgagata
     2761 gatatcgata acggcgagaa ggaccccggc ccctatgagg ctgtagccga cgaggagatt
     2821 ctccccaaag acgatgccgg cggtaatcaa ggcaagggag atggcgacga gtcccgagag
     2881 gtcttccctg ggctttttcc tgcctgcaat aaacagtaac agtgccacta tggccaggat
     2941 tgttatggaa attgcgagat acccttggga tgcatccatg gaaaacaggt acatgcacga
     3001 atattttatt tcttccatga attttcgata gttatggaca atcgggaact attccttaat
     3061 caatagtatt tgaaatctaa aatttcaggt gcaaagaagg actgatcatc ccggcattgc
     3121 cacgaagaca gttcgtactt ttttcccaga tttgaaagta tcccatttct ctccctcata
     3181 tcataaggaa tccgtccggt acaagactgg tggcgagcat gaccatgttg atctcctggt
     3241 gctctgtcca ggttccagcc ccctggttgt cctcatcaag ccagaacagc tctatctcct
     3301 ctgcgagagg catatatttc ctgagctccg caaccttccg gtgcagggaa tccttcgttc
     3361 caaacacagc cgggtaccct tcaatcattc cggataactc ttcgagccct ttcatcccga
     3421 tcgagagccc gagttcccgg aaggcgaggc ggtatccggc aggcagctgc aggagcctgc
     3481 tcctcttcac tgtccgcatt ccctggaggg ctgactctgt gacactctcc agcaaatccg
     3541 ggtgagcaaa tccgccctgc accatcagtt gtgcaatccg tgaggcatcg aaaaggaggc
     3601 ccccgatgcc gaggggatca tccgtcgccc agtcctgcat ccggcacatc tcttccatct
     3661 ccgcaatctc cgtgaatagg tttacctttg gcgatggttg ctggaaagat tccagcgtca
     3721 tctggagatc acggtaggtg accaacccat cgatgggatc gtgcaggccc atggaggaga
     3781 cctggggatg ggacagatcg acactcatct tccagaacat acgtttccga cctccagagg
     3841 ctggaaggta ggtgaatcgg gcatgggccg tctttgctaa ttccagcgcc caccgggtat
     3901 actgcggatc cccatacatc cggcttacac ggttgagggc atgcatccat tttgtcaggt
     3961 agtggtagta ctgcccatcc cggtcccatt cgagccgctc atcgaacggt tcgcccgaca
     4021 tgcgttcgtt cagttttttc ccaatccgga ggcccccaat ggtcggatgc agttcgccct
     4081 gctgctcatg caggccgctg atccagcctt tccgagggtc gtcttcacgg tgccggccga
     4141 ggacatggtg cacctggtcg acaaggcgca atgcaaggtc cctgtatgct gcatcatttg
     4201 tccggtgata cagttcaagg tagttgcata ccgcaaacgc atccgtccac aggtatcgtc
     4261 gcggagacgg agaaggagga tcaagccctg tcatgcgggc aaactcctgc atgatcccgt
     4321 ggacaaggga aataggatcg tgtttggtca ttctatcgcc ttgttgtaca atgagcaaaa
     4381 attcttttct tcccctctga tgtctttttt cagggtcccg tgagctattc cccctggaca
     4441 accgggggcc tgaccattcc ggtatgcaga atccctgatc ccggaaggct gacctgttcg
     4501 acaacggtaa acccaaagtg ctcgtacaga ccgatgttct tctcgttctg agtattcaga
     4561 tagcaggcta tctgcttcct gtccatccag tcaagcatgg gccggatcag cttccctcca
     4621 tatccttttc cgtggaggcg aggatcaaca ccgatgaaga agaggtagca atgggatggg
     4681 aagatgcttc ttatgataga catcgacctc ttctgcaaag gcaagaagcc gtttcatggc
     4741 gtccttcgaa agccctttct gcagcgccat cccccctgac cgcatcgccc tccagaacgt
     4801 cacctccgac cggttcgagg gaagccagac ggcaactccc tcaagggtgg gggatgtggc
     4861 atacacccgg ccatagttca ggccgtaacg gaggtggaat tcgaagatgt tccttccctc
     4921 ctctctcctg gtagtgtcgt cagggaggac atacgtcagt ttggcatcgt tgaagaatgc
     4981 ttcaacgagc atttcgcttg ccggtccgat ctgttctttc gaaagacgga taatagcccc
     5041 aggttgttca accatattcc cttcacgaaa agaaaatagc gagctttggt atatgattcc
     5101 tccggcacca ttcctttaac gtgagggtga ttcaatccct actcaaggat accatgaagt
     5161 tcagccagga agttctggat gcattcccgg ggatcggtgt ggccgaaggg aatatccggt
     5221 ctgtgcagat tgcccatgag agtgccgggc tggaaacact aaaacgggag accatccagg
     5281 aggtaaaatc ccgctatacc ctggagcagg tgaaggacga tccagtattc agagcgtaca
     5341 gggacttttt ctggagtgtc ggggtagacc cgaccaagac caggccggca tccgaagccc
     5401 tggtaagaag gatcctttca ggtgggaaac tcccgaggat caatactgcc gttgactcct
     5461 acaacctggc atctgcccgc tccggggtgc caatcgcagc attcgattct gatgccctga
     5521 caggggatct gacgctccgg ttcgccaata acggggaatc tttccttggt atcggaatgg
     5581 agaagcaggt agtgcttcag aagaaccagg tcatcatgac cgacgaaaaa cagatcgtcg
     5641 cgatctaccc gtaccgtgat tccgatacga ccaaggtgac cgcggaaaca cggaatatcc
     5701 ggatcatcac ctgcggggtg cctgggatcg ggcgggaact ggtttccggg gcattcgacc
     5761 tttgtgccaa gtacctggag aaatataccg gcgggatgtc taaaggtgca gagatattcc
     5821 caaagaaatg attctgatac cggggcagga taatcatgta tttctggctt tcaccgctaa
     5881 aaattcatga aaaacgtctg ggggaggtta ctccaacgca ttcatggggc atccacttca
     5941 gactttaatg agcccttttg caaacgtccg ggctctctcc aggtcctgct catcggggtg
     6001 tcccttgttc gcccacgtca atcccagcag gccggctgca ccggggcacc ggaattctcc
     6061 tacgacccgg gctcctttcc ccgccagcag ctctttcatg ggcttttcat acttttcatc
     6121 agggctccct gccgtcgaga agacgaacat gtccttctcc agccggggga ttttctcgag
     6181 gagcttatac aggtccttgt ggtatttgcc ggcgtagata ccggaaccga taccgatcag
     6241 gtcgtatccc gtgagatctt ccggctttgc ctcttccact ttcttgaggg tcgcgttcat
     6301 ggcttcggcc atcgcttttg cgaccttctc ggtattcatc ttgctgtaag atttgtaaat
     6361 gatgagggta ttcatgctgt tatctcttcg atagatggca taaaggtgat tgtgaagagt
     6421 cctgctgtcg gtctcagcga gaggtatcct gataacgacc gcggtctgca ggggactggt
     6481 gagatgatga ttctcggagc atgctccctg ctttcccgga cacggcgaat ctggcaccat
     6541 gattcagacc gatgcaatga taggcatcga ttcttcagaa caacgtgccc ttggtttcaa
     6601 tagtacagac gtggacccgc tatgacacac ttctatttat attctatagc gctatagact
     6661 atagcatgca aaagggaacg gaaaaggctc ctcctcccaa caaagtgaag aggtcaggaa
     6721 acacccgaga gcagccagga gatggttcca ccaatcggac gggtcttctc ccgactcccg
     6781 ggcgaatgga gttttcggac aggaccacca tccagatttc caaggatacc aagagagacc
     6841 ttgacaggat aaaacgagaa tccggaggga agacctacga tgatgccatc cggttcctgc
     6901 tcgatgagag gagaaggctc cgcccttcaa cattcgggct tctctccggt gcaggaccgt
     6961 ttgtccggga cgaggaggag gattctcatc ggattcgtcc ttgatacctg ggtctgggtg
     7021 gaatactggc gggggaatac ggccatgcga ccctgggtcg aggagcaggg tcctctcttc
     7081 agctcgacaa tcacctttgc agaaacggtc aggtatttta caatccaagg gatcgatccg
     7141 gctaccctcc tccactgcct ggacgatatc cgtacacgaa gtacggtcct tccgttagat
     7201 gagcaacttg ccatctttgc aggacacctg aagaaccgcg aagtggaggg aatcgccgat
     7261 gcgatcgtcc ttgctactgc ccgggcaggt ggccaccagg tcgtgaccgg ggacccgcac
     7321 ttccgtgatg tggaggatgc ggtatacctg ggcgaggagt gagggatgtt accgcgtacc
     7381 gtccggtaac gatccgacca tcgatgcgga tgtccggacg ggtacgaccc ccgaggcaga
     7441 atgactcacc ccaccaggta tcccatcaag cctcgggggg atcaagggag gggggccctg
     7501 ctgacacgcc tggcattgcc ggagaacttg cggagaagaa gggaatggac aagactaccc
     7561 tgcttgagca cattcataaa gcggagaagc ggctgatcgg gcatatcctc gcacaaggtg
     7621 cgtaagcgac ccgatcaatc gtccttcata ttcctatccc aaccatcggg ccgtatacga
     7681 tgacaatata gtacactccc acaatgatga acacggcagc gactgcccac ttgatccact
     7741 tctcgttctt ctgtaccgca gtcatcatcc cagaaacccg gttcaccccc gagacgagca
     7801 cgagtgagat gatgatgacc ggaagggcgg ccgcgatgcc aaagaccgcc gggacgcgga
     7861 tcg
//
LOCUS       DAOOCO010000082         7850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8729_length_7850_cov_5.324439, whole genome shotgun sequence.
ACCESSION   DAOOCO010000082 DAOOCO010000000
VERSION     DAOOCO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8729_length_7850_cov_5.324439"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..624
                     /locus_tag="PK154_05850"
     CDS             25..624
                     /locus_tag="PK154_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10621.1"
                     /translation="MGAKIIALLGSPLLSGNTAKLLDRAIQGARDAGAEVEKIEVTNL
                     NIRACREIMFCREHETCAMKDDMQGIYGKFRDIDGVILATPVMTMGIPGMLKSFIDRF
                     QVYFMAKYVRKKPLVIEEKRKSRLGLYLGISGMNLPSVFEGAKMTVHAFFDIIDVQYW
                     DDLLIRDMDTLQDLSRHPELMDDAYAKGRELGRHLMGRE"
     gene            complement(625..1290)
                     /gene="radC"
                     /locus_tag="PK154_05855"
     CDS             complement(625..1290)
                     /gene="radC"
                     /locus_tag="PK154_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW10622.1"
                     /translation="MKKMQDIEEQDRPREKIRKRGVTCLSDQELIVTILGSGVPGRNV
                     RDLAKEISDMIERNPDAVGYHELAGIKGMGPAKISQIVACLELGRRRYCQQGRKPKIS
                     SPSDVLPLVSHLSGKKQEHFLCITLNGANEPIETRTISMGLLNHSLVHPREVYADAIT
                     DRAASIICVHNHPSGTLEPSSQDLTITRQLADAGTIVGIRLLDHLIVTDSGWLSMKER
                     GLI"
     gene            1434..1934
                     /locus_tag="PK154_05860"
     CDS             1434..1934
                     /locus_tag="PK154_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW10623.1"
                     /translation="MATMENAMEAYAGESQANRKYAAFADQAESEGFKNIARLFKAAS
                     EAEAVHAKKLLKAMGTIKTTKENIQGSISGETHEYTEMYPAFVKEAEAEKKSDVALVF
                     TYAMKAEEVHANLYKEAMNALNHGQDLSNRAVVLCPVCGNIFLGPAPEKCPICNAVRK
                     VFKTIE"
     gene            2087..3235
                     /locus_tag="PK154_05865"
     CDS             2087..3235
                     /locus_tag="PK154_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW10624.1"
                     /translation="MPKVTVYSTQNCPYCRMVKAFLERQGVDYLPVDVGTDKEQAKKM
                     IELSGQYGVPVVTVDDEVIVGYDAQRLNELFGEEKGGVVFDVLIIGAGPAGLTAGVYC
                     ARKLLKTLIVSENIGGQASESWAIENYMGYRMITGDDLMRKFEEQVRSLDLHLEIDKV
                     LILDRSGNEFIVNTATGAAYRARAVILTQGKQPRKLGVEGEERYLGRGLSVCSTCDGP
                     LFRDKVVAVVGGGNSALQTAIEMSGIAREVHLIVRSTIKADEVYVRAFEKKSNIITHM
                     NSVVSRLQGNSMLSGITLKDRASGKETDLLVNGLFAEIGWIPNTAFLEGIVDMNPDME
                     VVIDINCHTSVPGIFAAGDITNIRTKQIITAAGEGAKAALSAYEYLMK"
     gene            complement(3247..4479)
                     /locus_tag="PK154_05870"
     CDS             complement(3247..4479)
                     /locus_tag="PK154_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW10625.1"
                     /translation="MNPDDDSESEFKNLVLRSCPFHDGETIDIAGIRITRYSGEFWTS
                     AQRKASSVHEISYRACFKPQLPHFFIKNLTGVGSRIYDPFSGRGTTVIEAGLLGRQIA
                     SNDANPLSRILTRPRFFIPAEKQVAARMAEIPAGTGERAEIDLSMFYDQKTEGEIVSL
                     RQYLINREETGRMDSVDSWIRMVATSRLTGHSAGFFSVYTLPPNQAVSPERQVLINQR
                     RAQVPEYRNIRDLILRKTRSLVRDLTPEERTRLNNAGKSGLFMTGDSGSTTPIPDESV
                     TLTVTSPPFLDTVQYAKDNWLRCWFCGIDAVAVERGLTVTRSLADWCNAMGSTFRELF
                     RITVPGGYVAFEVGEVRGGSILLDEQVLPVGAAAGFVPLGILVNLQQFTKTSHIWGIG
                     NNASGTNTNRIVVFRKEV"
     gene            4582..5136
                     /locus_tag="PK154_05875"
     CDS             4582..5136
                     /locus_tag="PK154_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10626.1"
                     /translation="MREIPRKLVHIIFGLGIAALIQLTPLHIALPVLMTGTFAGIIIR
                     DALVRGFRVPLISVLVDKLERENVRPGKGALSFALSSLFCLVLFEKEVVVPAVVTLSL
                     LDGVATLAGYYFGKHPVVGKKTAEGTIAGIGVTFFALLPLLPPVQAAVSVLLAGITEL
                     LSPVDDNLTIPVIVCIALTLMARI"
     gene            complement(5151..6617)
                     /locus_tag="PK154_05880"
     CDS             complement(5151..6617)
                     /locus_tag="PK154_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPW10627.1"
                     /translation="MREISVKVFRFDPSSDTTPHLQNYLVSVKEGARVLHVLHAIHDE
                     HDPSLSYRYCCGSGQCGSCAVKLNGQPVLACMEEARDEMIIEPLDLPIKKDLIVDMEP
                     YLDRISSLVPGAEFRMPAPDEVEAIKPLRSCIECLACVSACPALKVIDFAGPAAMRED
                     MRLSLDPRDTCDRVTEAVSEGLFTCTSCQSCWKVCPKNIEIPGKAIEKLRALANRKGL
                     TLPRHQEVEKLVRETGRSVDRTGTTFLEQVPEILEPEGEVKATVGFFVGCMYNMRLPQ
                     TALDAMEVLRRNGIRVIIPKEQVCCGSPLIRTGQIEFLDTLKSRNINAYVFRNIDTVM
                     TMCAGCGSTLKNDYRTPFKVMDINEVLLRYGIEPPARLPIKATYHDPCHLLRGQGIRT
                     QPRELISKVVDLVEMPAICCGSGGGVRSGMPQEAAALGENRRNEIDKTGADLVISSCP
                     FCEFHIKEHTDKPVKNVATVLLEGYKEKDRRTAQKETC"
     gene            complement(6614..>7850)
                     /locus_tag="PK154_05885"
     CDS             complement(6614..>7850)
                     /locus_tag="PK154_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPW10628.1"
                     /translation="DRTGHEMISTLVDRLHGTGVRIENEVTVISLLQEGQRVSGAIGL
                     DRQGAIRVMLADATILATGGGTRIYDISTNSSSGTGDGYAIGYNAGAELIDMEQIQFH
                     PTGAVYPYDARGRLVTEAVRGEGGLLKNVEGERFMKRYDPERMELSTRDVVARAIATE
                     ILEGKGTKNGGVYLDVTHLPRSQIESRLPVMLEQFLKFGIDIRDVPMEVAPTAHHIMG
                     GLRITTMGETTLPCLYACGEVSGGVHGANRLGGNALAETQVFGKRAGEAAGRVKERKK
                     AADQKMIEDAEQRISSFNQGDVAPSAIVRSLQTTMWEGAGIFRNGPDLQKTLGIIRHL
                     QTLNPKAASPANFAECCILQNMLTTASLVVRSALLRKESRGAHLRRDISQNWDPEHSP
                     YGHTYLSLSREGIETRAVT"
BASE COUNT         1753 a         2108 c         2114 g         1875 t
ORIGIN      
        1 aaaccttacg taacagtcta attcatggga gcaaagataa ttgcgcttct cggaagccct
       61 cttttgagcg ggaatacggc aaaattgttg gatcgggcga tacagggagc gagggatgca
      121 ggggccgagg tggagaagat tgaggtcact aatctcaata tcagggcatg ccgggagatc
      181 atgttctgca gggagcatga gacctgcgca atgaaggacg atatgcaggg aatatacggg
      241 aaattccgcg atattgacgg ggtcatcctc gccactcctg ttatgaccat gggaatcccg
      301 ggaatgttga agtcgttcat tgaccgtttc caggtctact tcatggcaaa atacgtgagg
      361 aaaaaaccac tggtcatcga agaaaaaaga aaatccaggt taggcctcta tctcggaata
      421 tccggcatga atctcccctc tgtctttgaa ggggcaaaga tgacggtcca tgcttttttt
      481 gacatcattg acgtgcagta ctgggatgat ctcctcataa gggacatgga caccctccag
      541 gatctttcaa gacatcccga gttaatggat gatgcctatg caaaggggcg tgaactcggc
      601 cggcacctca tgggaaggga atgatcagat gagcccccgc tctttcatcg agagccatcc
      661 ggaatcggta acgatcagat gatccaggag acggatcccg actatggtcc ctgcatcggc
      721 cagctggcgg gtgatggtaa gatcctgact gctcggctcc agggttcctg aagggtggtt
      781 gtggacgcag ataatagatg cagcccggtc ggtgattgca tcggcataga cctcgcgggg
      841 atggacaagg ctgtgattca ggaggcccat cgagatggtc cgtgtctcaa tcggttcatt
      901 tgcaccgttc agcgtgatgc agagaaagtg ctcctgtttc tttcccgata gatgagagac
      961 gagcgggagt acatctgaag gtgaactgat ctttggtttt ctcccctgct ggcaatacct
     1021 tctcctgccg agctcaaggc aggcgacgat ctgggatatt tttgcaggcc ccatcccctt
     1081 gatcccggca agttcatgat atcctactgc atctggattt ctctctatca tgtcagagat
     1141 ctcttttgca agatccctaa cgtttctacc aggcactccg cttccgagga tggtaacaat
     1201 cagttcctga tcgctgagac acgtgactcc tctcttccgg atcttctccc tcggccggtc
     1261 ctgctcttcg atatcctgca tctttttcat gttccacctt cgaggacaat cgcgatatca
     1321 tctgtatctt cccggaatga gaaaaagagt atgttctcct ctcagtggtc ggatcacctg
     1381 cagtgtcagc ctgaaaggat accttgatgt aattcgttgc cacagataag agcatggcaa
     1441 caatggagaa tgcaatggag gcatatgccg gggagtcaca ggcaaaccgc aagtatgcag
     1501 cctttgcaga tcaggctgag tctgagggtt tcaaaaatat cgcaaggctt tttaaggcag
     1561 catctgaggc tgaagcggtc catgccaaga agctcctcaa ggcgatgggc acgatcaaga
     1621 ccaccaagga gaatatccag ggttcaattt caggcgagac gcatgaatac accgagatgt
     1681 atcctgcgtt tgtcaaagaa gcagaggccg agaagaagag tgacgttgca ctggtattta
     1741 cgtacgccat gaaggcagaa gaggttcatg ccaaccttta caaggaagca atgaatgccc
     1801 tgaaccatgg acaggatctc tcgaaccggg cggttgttct ctgccccgta tgtggcaaca
     1861 ttttcctcgg tcccgcccct gaaaagtgcc ctatctgcaa tgcagtcagg aaagtattta
     1921 aaacgatcga ataacttcca ctacccccaa ttcatttttt ttccctccgg atatcggtct
     1981 catctcatgt tattcaggga acgatgagcg gttcctccca ttctgttctc ctgatactgt
     2041 ccagctccca cactttaatt accggataaa ggcaaactcg atttgcatgc cgaaggttac
     2101 tgtatactca acccagaact gcccctattg ccggatggta aaagcgttcc tcgaacgaca
     2161 aggggtggat tatctccctg tggatgtcgg gactgacaag gagcaggcaa agaagatgat
     2221 cgagctatcc ggacagtacg gggtaccggt cgtcacggtt gacgatgaag tgatcgtggg
     2281 atatgatgcc cagcgcctga atgaactgtt cggagaagag aaaggagggg tagtcttcga
     2341 tgtcctgatt atcggggcgg gcccggcagg cctgactgca ggagtatact gtgccaggaa
     2401 attattgaag actctgatcg tcagtgagaa catcgggggc caggctagtg agtcctgggc
     2461 gatcgagaac tacatggggt accggatgat taccggggat gacctgatgc gaaagttcga
     2521 ggagcaggtc aggtcactcg atcttcacct cgagatcgat aaagtgctga tcctcgaccg
     2581 gagcggcaac gaatttatcg tgaacacggc gacgggagca gcataccggg cccgtgcagt
     2641 tatccttacc cagggcaaac aaccgaggaa actgggggtc gagggggagg aacggtatct
     2701 ggggcgaggt ctctcggtct gttcgacatg cgacggcccg ctcttccggg acaaggtggt
     2761 tgccgttgtc gggggaggga attctgcgct ccagactgcg atcgagatga gcggaattgc
     2821 ccgggaggtg cacctcatcg tgagaagtac tataaaggcg gatgaggtct atgtcagggc
     2881 atttgaaaag aaatcaaata tcatcaccca tatgaacagc gtggtgtccc gtctccaggg
     2941 caacagcatg ctttccggaa tcaccttgaa ggaccgcgct tcaggaaaag agaccgatct
     3001 gctggtgaac ggattgtttg ccgagatagg atggatcccc aataccgctt ttctggaagg
     3061 gatcgtggac atgaatcctg acatggaggt tgtgatcgat atcaactgcc acaccagcgt
     3121 tcccggcatc tttgctgcag gggatatcac aaatatcagg acaaaacaga ttatcacggc
     3181 ggccggggaa ggggcaaagg cagcactctc cgcatacgag tacctgatga agtgatccct
     3241 ctccccctag acctcttttc tgaagaccac gatccggttg gtattcgtcc ccgaggcgtt
     3301 gtttcctatc ccccagatat gggatgtttt ggtaaactgc tggaggttga caaggatacc
     3361 aaggggcaca aatcctgccg cagcaccgac gggaagcacc tgttcatcca gcaggatact
     3421 ccccccccgt acctctccga cttcgaatgc gacatacccc ccgggaaccg tgatcctgaa
     3481 cagttctcgg aaggtactcc ccatcgcgtt acaccagtct gctagagacc gggtcacggt
     3541 aaggccgcgt tctactgcta ccgcatcgat cccgcaaaac cagcaccgta accagttgtc
     3601 cttcgcatac tggaccgtgt caaggaaggg aggtgaagtg accgtcaggg tgaccgattc
     3661 atccgggatt ggtgtggttg agccggaatc tcctgtcatg aagaggccgc tttttcctgc
     3721 attgttcagt ctggtgcgtt cttccggggt gagatccctg acaagcgatc gggtctttct
     3781 caggataagg tcccggatat tccggtactc cggcacctgg gctctccgct ggttgatcag
     3841 gacctggcgt tccggggaga cggcctggtt tggagggagg gtatataccg agaagaagcc
     3901 tgcggaatgg ccggtcagcc tgctggttgc caccatccgt atccatgaat cgacagaatc
     3961 cattcttccg gtttcttccc ggttgatgag atactggcgt aaggatacaa tctcaccctc
     4021 cgtcttttga tcgtaaaaca tggagaggtc tatctcggct ctctcgccgg ttcccgctgg
     4081 aatctctgcc attcgtgcgg cgacctgctt ttcggcaggg atgaagaacc tgggccttgt
     4141 gaggatccgg ctgagggggt tagcatcatt ggaggcgatc tgcctcccaa gcaggccggc
     4201 ctcgatcacg gttgttcccc tccccgagaa cgggtcatag atcctggagc caacccctgt
     4261 caggtttttg atgaagaagt gggggagctg cggtttgaaa caggccctgt aggagatttc
     4321 atggacagaa gatgccttcc tctgagccga ggtccagaac tcaccggaat aacgggtgat
     4381 tctgataccc gcaatatcga tagtctctcc atcgtgaaaa ggacaagacc ggagtaccag
     4441 gttcttgaat tcgctctctg aatcatcatc gggattcatg gagattttcc ggatcaatta
     4501 atcagcagcc tttcttatca ataccaaccc tgaacccgcg atcggatacc ttatggggct
     4561 ttattaccaa ccaccaatca tatgcgcgag atcccccgaa aactggtcca tattattttc
     4621 gggctcggaa tcgccgccct tatccagctt acccctctac atatcgcgct tcctgtgctc
     4681 atgaccggaa cttttgccgg gatcatcatc cgggatgccc ttgtgcgggg gttccgggtg
     4741 ccgctgatat cggtccttgt tgacaagctt gaacgcgaga acgtccgacc gggaaaagga
     4801 gcactctcct ttgccctgag ttcgctcttc tgtcttgtac tcttcgaaaa ggaagtggtg
     4861 gttcctgctg ttgtaaccct ttctctcctg gatggtgtgg cgacactggc cggttattat
     4921 tttgggaaac atccggttgt cggaaaaaag actgcggaag gaaccattgc agggattgga
     4981 gtcacctttt tcgcactgct tccgctcctg cccccggtcc aggcagcagt ttcagtactc
     5041 ctcgccggta taacggagct tctctcgccc gttgatgata accttaccat cccggtaatt
     5101 gtctgcatag cattgaccct gatggcaaga atctgaaaaa cagggatgac ctaacaggtc
     5161 tctttttggg cagttctccg atctttttct ttgtacccct caaggagcac cgtggccacg
     5221 ttcttgaccg gtttatcggt atgttccttt atgtggaact cgcagaaggg gcaggagctg
     5281 ataaccagat ctgccccggt tttatctatc tcatttcgtc ggttctcccc cagtgcggca
     5341 gcctcctggg gcatcccaga ccgcactcct ccgcccgagc cgcagcagat cgccggcatc
     5401 tctacgaggt cgacaacctt actgatcagt tcccgcggct gtgtccgtat cccctgtcct
     5461 ctgaggagat gacagggatc gtggtaggtg gcttttatcg ggagtcgtgc agggggctca
     5521 atcccgtacc tgaggagtac ctcgttgatg tccatcacct tgaacggtgt ccggtaatcg
     5581 ttcttcaggg tcgatccgca ccctgcacac atggtcatca cggtgtcaat attccggaat
     5641 acataggcgt tgatattcct gctcttgagg gtgtcaagaa attcaatctg gccggtcctg
     5701 atgagaggtg atccgcaaca gacctgctcc tttggtatga taacccggat gccattccgc
     5761 cttagaactt ccatggcatc aagggcagtc tggggaagac gcatattgta catacagccg
     5821 acaaagaacc ccacagtggc ttttacctcc ccctcgggtt caaggatttc tggtacttgc
     5881 tcaaggaacg tcgtcccggt ccggtctaca ctcctcccag tctcccttac cagtttctct
     5941 acctcctggt gacgaggcag ggtaagccct ttccggttgg caagagccct cagtttttcg
     6001 atagcttttc ccgggatctc aatatttttc gggcagactt tccagcagga ctggcatgat
     6061 gtgcaggtga acagtccttc actcactgct tcagtgacac ggtcgcaggt atccctggga
     6121 tctagggaga ggcgcatatc ctcccgcatc gcagcaggcc ccgcaaaatc gatcactttg
     6181 agtgcaggac aggccgatac gcaggcgagg cactcgatgc agcttctcag tggctttatc
     6241 gcctccacct catcaggagc aggcatccgg aattctgcac ccgggacaag agaagagata
     6301 cgatcgagat agggttccat atccacgatg agatcttttt tgatcgggag gtcgagcggc
     6361 tcgataatca tctcgtcacg agcttcctcc atgcaggcga ggaccggctg accgttcagc
     6421 ttcacggcac aactcccgca ttggccagaa ccgcagcagt agcggtacga gagtgaggga
     6481 tcgtgctcat catggatggc atgcagcaca tggaggactc gagctccttc cttgacactt
     6541 acaaggtaat tctggagatg gggggtggta tcggatgatg ggtcaaaccg gaataccttg
     6601 acggagatct ctctcatgtc acagccctcg tttcgatccc ttcccgcgac agcgagagat
     6661 aggtatgccc atagggggag tgttccggat cccagttctg ggagatatcg cgcctgagat
     6721 gtgctccccg cgattccttc cgaagcaggg cagatctgac caccagcgag gcagtggtca
     6781 gcatattctg gagaatgcaa cattcggcga aatttgccgg tgatgctgcc ttcggattga
     6841 gtgtctgcag gtgcctgatt atccccagtg tcttttgaag gtcgggaccg ttcctgaaga
     6901 tacctgcccc ctcccacatt gtggtctgga gagagcggac gatggccgat ggcgcaacgt
     6961 ccccctgatt gaatgaggag atacgctgtt ctgcatcctc gatcatcttc tgatccgctg
     7021 cctttttccg ctcttttacc ctccctgccg cctctcctgc tcgtttacca aagacctgtg
     7081 tttcagcgag ggcatttcca ccaagccggt tcgcaccatg aacccccccc gatacttccc
     7141 cgcaggcata aaggcaggga agtgttgtct ctcccatcgt tgttatccgg agccctccca
     7201 tgatatggtg ggcagtaggg gcgacttcca tggggacatc acggatatca atcccgaact
     7261 tgaggaactg ctcgagcatg accggcaacc tgctctcgat ctgggatcgg gggaggtggg
     7321 taacatccag atacacgcca ccatttttcg taccttttcc ctccagaatc tctgtcgcaa
     7381 tcgcccgtgc gaccacgtct cgtgtggaga gttccatgcg ttcgggatca taccgtttca
     7441 tgaaccgctc accctccaca tttttcagga gacccccctc cccgcgaact gcctcggtga
     7501 cgagtcttcc ccgtgcatca tagggataga ccgctcctgt cggatggaac tgtatctgct
     7561 ccatgtcgat cagctcggca cctgcgttgt acccgatggc atatccgtca cccgtcccgc
     7621 ttgaagagtt cgtggaaata tcatatatcc gggtacctcc ccctgtggca agtatggtgg
     7681 catccgcaag catgacccgg atcgctccct gccggtcgag accgattgcc ccgctcaccc
     7741 tctgaccctc ctgcaggagt gaaatgacgg tgacttcgtt ctcaatccgg acaccggtac
     7801 catggagccg gtcgaccagg gttgatatca tctcatggcc ggtacggtca
//
LOCUS       DAOOCO010000083         7837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8742_length_7837_cov_4.644950, whole genome shotgun sequence.
ACCESSION   DAOOCO010000083 DAOOCO010000000
VERSION     DAOOCO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_7837_cov_4.644950"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PK154_05890"
     CDS             complement(<1..1030)
                     /locus_tag="PK154_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10629.1"
                     /translation="MYAIRFYRIYDVGDEIDLSALEKALASSMAIARTGFVRVRPTSM
                     TIEEPPLLLRLQPAYAEIGGTGCTFTVVSRVFDFGAMSICLILEEMDAPASALQDAAA
                     RFSGEGVLDPHFSAALLQVRTILASIVGDRSIDEDFYDDYTIYLSERLDESVDPVEIL
                     LGEKEDFSPEIRQDTLQNQLSYARDDRAILSWNGAILISPQPPSDLIELIEFAAVQVL
                     ELRFYDRELSRQMEKMYDDIEVADRLFWYRKVRQYHSLMKVLMRTQTEVSEIVEKVNN
                     LIKITEDVYYARVYAMALKVLRSQQWTESLNRRIGIIRENYRMLSDEVDVQHSNFLEW
                     VVIILIAFE"
     gene            complement(1107..1430)
                     /locus_tag="PK154_05895"
     CDS             complement(1107..1430)
                     /locus_tag="PK154_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HPW10630.1"
                     /translation="MLHQFMMFSVNRCINRLSSTIILNDTTEQAINEYVKENSKEYYE
                     IPPGFEFRGVIILLKNPMLMGFKIKKQKILMPFVKPCFGPMLIELAAWDGEFEQIREQ
                     LSKVS"
     gene            complement(1565..1882)
                     /gene="eif1A"
                     /locus_tag="PK154_05900"
     CDS             complement(1565..1882)
                     /gene="eif1A"
                     /locus_tag="PK154_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HPW10631.1"
                     /translation="MTSTTHSQNGPGTEIVRVRLPKKREKEIFARAESMLGANHISVR
                     CADGVTRMGRIKGTIKKRLWIREGDVLIITPWSFQDEKCDISYRYTRPQVEWLQRNGY
                     LKS"
     gene            2161..2424
                     /locus_tag="PK154_05905"
     CDS             2161..2424
                     /locus_tag="PK154_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW10632.1"
                     /translation="MQGSKLYVGNLTYSVNEEQLKKLFSAYGDVKEVRVIERKGFGFV
                     EMATPEDAEKAKAALNETMFEGRTLKIDEARPPQPRTSFRPRY"
     gene            complement(2725..3195)
                     /locus_tag="PK154_05910"
     CDS             complement(2725..3195)
                     /locus_tag="PK154_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW10633.1"
                     /translation="MFLEKILYATDFSETSASALGYVIQLQTAGCKEVILVHVLDARK
                     TEEVLAEPSGGDEPSGEYERQIAGRILNNARQELKTIERALEVEQFLVRTFLVNGNPS
                     REINRIADAECAGMIVIGSHGKTNLLHRLMGSISLGVIENATRPVLIVRRSEEQ"
     gene            3344..3922
                     /locus_tag="PK154_05915"
     CDS             3344..3922
                     /locus_tag="PK154_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10634.1"
                     /translation="MKVVAFNGSPRKEGNTRHLLEHVLAPLKEEGISTELVQVGGKKV
                     HGCTACGKCFENQDRRCVIEDDIVNGCIGKMAAAQGILIGSPTYFADVSAETKALIDR
                     SGFVGMANGGLYARKVGAAVVAVRRAGGIHVFDTINHFFGISNMYTVGSSYWNIGMGL
                     NPGEVDEDQEGIGTMENLGRNMAWILKKIHGT"
     gene            4104..5300
                     /locus_tag="PK154_05920"
     CDS             4104..5300
                     /locus_tag="PK154_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW10635.1"
                     /translation="MEGIVGTVEFQMSLLLFVALAGYLLASRINQSAVVGEILVGLVV
                     GPSVLGLITYTDFVASIAHLGAVILLFVIGLEFEIREIFRLKYAIIALVGVIVPWLGG
                     YWVSLLFGFNTASAIFIGTALTATSIAITANILKELGKLDTEVAKAIIGAAVIDDILS
                     LIALSATTDIVSGTISFSSVTRLVIIAIIFVAGGAWIGRTWVSRLIVKMDESEIAGKY
                     PEFVFIFAMMFAFFYAMCAELIGLSAIVGGFIAGVSFVGVNLKRSRNLREGAEYLQMI
                     FAAVFFVSLGVLADVHQLTVPILGFLIVLTLVAIITKVVGCAIPGRLTGLCGKDSLIL
                     GFGMAPRGEVAMIVALIGLEQGVIGQEVYVVIILMSLLTTLITPIIYRNWLYKGEYCT
                     YPQGEP"
     gene            5467..5949
                     /locus_tag="PK154_05925"
     CDS             5467..5949
                     /locus_tag="PK154_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW10636.1"
                     /translation="MPFYCQQCGECCTQMGYVHEIKADLGNNEYLIRNVYTGEETRSR
                     VSPLMLHLFEDRSTFEQWPKACPFFRCNADGRKGYCTVYPTWPEICQEFGCWRLLILD
                     ANGRRAGRVMIGTYLACEDATLRQVWDYHIREMEVADDAEWIRKVTRILTRLGYTVIR
                     "
     gene            6269..7567
                     /locus_tag="PK154_05930"
     CDS             6269..7567
                     /locus_tag="PK154_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW10637.1"
                     /translation="MLAASVAVSSAMITTVPVTEAVPVTLPAPGSEQGYFSINSVPSG
                     GDVYFDSAFWGETPVVVTVSTTGTPTHTIRITLPGYEEWTSTYNGNPMAGQTIPITAQ
                     LVPSAQTGNIQVVSTPSGAMVSLDRSQSANTPYVFYNIPVGSHEVSVFMSGYQTFYTS
                     VNVNKGQTAYVNAILQPESTTGTLSVSSSPSGAAVYVDGIYHGVTSTTVGNLIPGSHS
                     VRLSKAGYLDWTGSVSISAGSTTYLDPSLVKDQQPQYATVSITSNPAGASVYGDGVYV
                     GQTRAGSPLVFTSVKPGTHTLLLTKSGYQDYSVTRTVSAGQNYDVDVTLNPVQNPTTG
                     GISIISAPSQAEVYLNNAYKGLTPVTLDSLAPGSYTVLLRLSGYQDWQATQQVTAGQT
                     AQISATLTPSATPTPTQSGLLPLVVLAGIGIVFLALRKRS"
     gene            complement(7649..>7837)
                     /locus_tag="PK154_05935"
     CDS             complement(7649..>7837)
                     /locus_tag="PK154_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPW10638.1"
                     /translation="ERLLGGVCSGIGHYFDIDPNMIRIVWVVLTLLSIGVGVIAYLIA
                     WLLIPEEEDDRAEVISVT"
BASE COUNT         1711 a         2135 c         2159 g         1832 t
ORIGIN      
        1 cttcaaaggc gatcaggatg atcaccaccc attcgaggaa attggagtgc tggacatcca
       61 cttcatcgga cagcatccgg tagttctcgc ggatgatccc aatacggcgg ttgaggcttt
      121 cggtccattg ctggctcctc aatactttca acgccatggc atagacccgt gcatagtaca
      181 catcctcggt gatcttgatc aggttgttga ccttctccac gatctcggag acttcggtct
      241 gggtccgcat caggaccttc atgagcgagt ggtactggcg aacctttcta taccagaaga
      301 gccggtctgc aacctcgatg tcatcataca tcttctccat ctggcgggag agctcgcggt
      361 cataaaacct gagttcgagc acctgcaccg cagcaaactc gatcagctca atcaggtcgg
      421 agggcggctg ggggctgatc aggatcgccc cgttccagga gaggatcgcc cgatcatccc
      481 gggcatagct cagctggttc tggagggtat cctgccggat ttccggggag aagtcctcct
      541 tttctccaag gaggatctcg accggatcaa cgctctcgtc aagacgttca gagaggtaga
      601 tggtataatc atcgtagaaa tcctcatcga tcgagcgatc gccaacgatc gacgccagga
      661 tggttcggac ctgcagcagg gcagcagaaa agtggggatc gagcacccct tctccagaaa
      721 aacgggctgc agcgtcctgc agtgctgatg caggggcgtc catctcttca aggatgaggc
      781 agatgctcat cgccccgaaa tcaaacactc tcgatacgac cgtgaaggta caacccgtgc
      841 caccgatctc ggcatatgcc ggctgaagac ggaggaggag tgggggctct tcgatggtca
      901 tggaggtcgg gcggaccctg acaaacccgg ttcgtgcgat tgccatgctg ctcgccaggg
      961 ccttttccag ggcactaagg tcgatctcgt cccctacatc gtaaatacgg tagaagcgga
     1021 tggcatacac aggggatcac ccgatctctt cagagttctg gaagaataga gcgggatagg
     1081 agataaggat tgagggatcc ggcaggttat gagacttttg acagttgttc ccggatctgt
     1141 tcaaattctc catcccaggc cgccagttcg atgagcatgg ggccgaaaca gggttttaca
     1201 aacggcatga ggatcttctg cttcttgatc ttgaagccca tgagcatggg atttttaagg
     1261 aggatgatga ccccccgaaa ctcgaaccct ggggggatct cgtagtattc cttgctgttc
     1321 tctttcacat actcgttgat agcctgctcg gtggtatcgt tcaggatgat ggtcgaactg
     1381 agtcggttga tacaccgatt gactgaaaac atcatgaact ggtggagcat cgtactacaa
     1441 aaatccgacg tccggggaag gttacgatcc agctgcctat agaatgtact ggtctaaaag
     1501 agggtttcga catcaggaga cgagagcggc ccctgtaatg ttacgatggc gctggggaaa
     1561 aggattatga cttgagatac ccgttccgct ggagccattc gacctgtggc cgtgtgtagc
     1621 ggtacgagat atcgcacttt tcatcctgga aactccaggg agtgatgatg agcacatcgc
     1681 cttcccggat ccagaggcgt tttttgatcg tgcccttgat ccggcccatc cgggtcaccc
     1741 cgtcagcaca acggacactg atatggtttg caccaagcat cgattcagcc ctggcaaaga
     1801 tctccttctc tctcttcttc ggcagccgta cacggacgat ctcggtcccg ggcccgtttt
     1861 gtgaatgcgt ggtgctggtc aggaaaacaa ctccgtcaat gaaggatatt ataaatgttg
     1921 ccaactggct ttaacctatc catggagtga gcctctgagc ctctgccccc ccctggcagt
     1981 accattttat ggaagggata cctctctccc cggtcaatgg gcgccttcct gcgatatggt
     2041 gggccggcgc tccctttgcg catgggtcgc cagcaattcc ccactcttcc tgcgttctgt
     2101 ctcctcctaa aagtatatgt ggaaaaggac cgatgtatag aggaaaagga acgtaatcat
     2161 atgcagggaa gtaaactcta cgttggaaat ctcacgtatt ctgtcaatga ggaacaatta
     2221 aaaaaattat tctcagcata cggggatgta aaggaagttc gtgtcattga gcggaagggc
     2281 tttggattcg tcgagatggc gacccccgag gatgccgaga aggcaaaggc agccctcaac
     2341 gagacgatgt ttgaaggccg caccctgaag atcgatgagg cacgcccgcc gcagccccgg
     2401 accagtttcc ggccacggta ctgatcgtct tctcaactct ttttttattt cacagaaacg
     2461 ggtcaccgga gatctccatc acgtgtggag acgggaactg atagagagcg gtaccctgtg
     2521 ttgccaggag ggcatttgcg tcctgaacgc ctggcccaaa agaagtgcca aggatcccct
     2581 ggctcatctc cgaccagttt ccgggagccc ggtctcgctg aaaggggaga tcttcctgaa
     2641 cggagagaga attaatctgc cctgatacct catcctgctg ggcatacgag aatcgccctt
     2701 tggaaaagca ggagatctca tctcttactg ctcttcactc ctcctcacga taagcaccgg
     2761 ccgggtcgcg ttctcgatca ccccaaggga gatcgaaccc atcagccgat gcaggagatt
     2821 ggtcttccca tgggagccga tcacgatcat cccggcgcat tccgcatcgg caatacggtt
     2881 tatctcccgg gatggatttc cgttcacgag aaaggtccgg accaggaact gctccacttc
     2941 gagcgctctc tcgatcgttt tcagttcctg ccgggcattg ttcagtatcc tgccggcaat
     3001 ctgccgctcg tactcccccg atggttcatc cccgccggat ggttctgcaa ggacctcctc
     3061 tgtctttctg gcatcaagta catggacgag gatcacctct ttacagcctg cagtctgcag
     3121 ctggatcaca taaccgaggg cacttgctga ggtctccgag aaatcggtag catacaggat
     3181 cttttcaaga aacatacctg cacttcacaa aaggactact ggccggctat accgcattaa
     3241 ggcatcggat tggtccggga tcctcatggt ggacaaagag ggatatcccc tgattatgcc
     3301 gtgggaaatc cttgaaatca agggatgtcc aagtacctag ggtatgaagg ttgtcgcatt
     3361 caacgggagc ccgcggaaag aggggaatac caggcacctg ctggaacatg tgcttgctcc
     3421 tctcaaagag gagggcatca gcactgaact tgtccaggta ggcggaaaga aagtccatgg
     3481 atgtaccgcc tgcgggaaat gttttgagaa tcaggaccgg agatgcgtga tcgaagatga
     3541 tatcgtgaat ggctgcatcg gaaaaatggc cgctgctcag gggatcctta tcggttcacc
     3601 gacgtatttt gccgatgtga gtgccgagac gaaagccctg atcgaccgat ccggttttgt
     3661 cgggatggcc aacggcggtc tgtatgcaag gaaagtcggg gcagcggtgg tggctgtccg
     3721 gagggcaggg gggatccatg tcttcgacac gatcaaccat ttcttcggca tcagcaacat
     3781 gtataccgtc gggtcgtcgt actggaacat cgggatggga ctcaatcccg gcgaggtgga
     3841 tgaggatcag gaagggatcg gaaccatgga gaaccttggc aggaacatgg cctggatcct
     3901 gaaaaagatt cacggtacgt aactcttccc ggccgaaaat acccagctcc ccggattcct
     3961 ggtgtccata tccccgggtc attccctgtg ccaggacttg tacggggacg ggcggtcctc
     4021 cttatgaaac agggggtatt acactgcagg gagcggcccg gtttctgcca tgattttata
     4081 cttttccagc tttattgcat tgcatggagg gtattgtcgg cacggtcgaa ttccagatga
     4141 gcctcctcct ctttgtcgcc cttgccggtt acctgcttgc atcacggatc aaccagtcag
     4201 cggttgtggg agagatcctg gttgggcttg tcgtggggcc gagtgtgctt ggcctgatca
     4261 cctataccga ttttgttgcc agtatcgccc atcttggggc cgtcatcctg ctctttgtga
     4321 tcgggctcga gttcgagatc agggagatct tccggttaaa atacgcgatc atcgctcttg
     4381 tcggggttat cgtcccgtgg cttggagggt actgggtctc gctgctgttc gggttcaaca
     4441 ctgccagtgc catcttcatc gggactgccc tgaccgcaac cagtattgcc atcaccgcca
     4501 acatcctcaa ggagcttggc aaacttgata cagaagttgc caaggcgatc atcggcgcgg
     4561 cggtcatcga tgatatcctc tcgctcattg ccctgtcagc aaccaccgat attgtgtcag
     4621 gcaccatcag tttctcatca gtcacccgcc tggtgatcat cgcgatcatc tttgttgccg
     4681 ggggtgcctg gatcgggaga acctgggtga gccggctcat cgtgaaaatg gacgagtcgg
     4741 aaattgccgg gaagtatccc gagtttgtct tcatctttgc catgatgttt gccttcttct
     4801 atgccatgtg cgccgagctg atagggctct cagcaatcgt cgggggtttc attgcagggg
     4861 tatcatttgt cggcgtgaat ctgaaacgga gcaggaacct ccgggaagga gcggagtacc
     4921 tccagatgat ctttgccgcg gtcttctttg tctcgcttgg cgtccttgcc gatgtgcacc
     4981 agctcacggt gcccatcctg gggttcctga tcgttctcac cctcgtcgcg atcataacaa
     5041 aggttgtcgg ctgtgccatc cctggtcgat tgacgggact gtgcggaaag gacagcctca
     5101 tcctcgggtt tggtatggca ccacggggtg aggttgccat gatcgtggcc ttgatcgggc
     5161 ttgagcaggg agtcattgga caggaggtct acgttgtgat aatcctcatg agcctgttga
     5221 ccaccctgat aacccccatc atctaccgga actggctcta caagggtgaa tattgcacct
     5281 acccgcaggg agaaccgtag tctctttttt cctggcatcc ctgtgttcat tcctttcccg
     5341 gtgttcattc cttagtatcc ctgggatgat caaaatcgga tcccttggga tgatcaaaat
     5401 cggatccctt gggatgatcc caatctattt ctctctcttg taccgagctc ttcaggatca
     5461 cggatcatgc cgttctactg tcagcagtgc ggggagtgct gcacccagat gggctatgtc
     5521 catgagatca aggctgacct tggaaacaat gaatacctga tacggaatgt gtataccggg
     5581 gaggagaccc ggtcccgggt ctctcccctg atgctccacc tgtttgaaga caggagcacg
     5641 tttgagcaat ggcccaaggc ctgcccgttc ttccgatgca acgccgatgg gaggaaaggc
     5701 tactgcactg tgtaccctac ctggccggag atctgccaag agttcggctg ctggcgtctc
     5761 cttatccttg acgctaatgg gaggagagcg gggagggtga tgatcgggac ctaccttgcc
     5821 tgcgaggatg ctaccctccg gcaggtctgg gactatcaca tcagggagat ggaagtggct
     5881 gacgatgcag aatggatcag gaaggtgacc aggatcctca ccaggctcgg atataccgtc
     5941 atccgataga ccggctgggg aggcagggaa tcccggttcc gattcaccgg cctatgagaa
     6001 gcagactttc ttcgaaagtt tccccacggg gctcttgcct gttctccgga atgagcagag
     6061 cggaaggagg aggtctggcc tttcctcctc tctgtttcat caatgatgat gggagatgaa
     6121 aaagatcaaa aagacctcat cttagttcca tggtatccga acttccggcg aatcggcagt
     6181 ttgaaatagt tccggaacgg catgttccgt tatagcagtt ggagatagta gaatgatgag
     6241 atcttctgcc attcttatga tggtactgat gcttgcagct tccgtggcag tttcttctgc
     6301 catgatcacc acggttcctg ttaccgaagc tgttcctgta acgctcccgg cacccgggag
     6361 tgaacaaggg tatttctcga tcaattccgt cccctctggc ggggatgtat acttcgatag
     6421 tgcgttctgg ggagaaaccc ctgttgtcgt gacggtatct acaactggta ctcccaccca
     6481 tacgatcagg attacccttc ccggttatga ggaatggact tcgacctaca atggcaatcc
     6541 catggctggc cagacgatcc cgatcacagc ccagctcgtc ccaagcgcac agaccgggaa
     6601 catccaggtc gtctctacgc catcgggtgc catggtcagc cttgaccgca gccagtctgc
     6661 aaacactccc tatgtcttct acaatatccc ggttggttct cacgaagtgt cggtgttcat
     6721 gtcgggttac cagacctttt atacctccgt gaacgtcaac aaggggcaga ctgcgtatgt
     6781 gaatgccatc cttcagcctg aatccacgac aggcaccctt tcggtgagct cttctccaag
     6841 cggagctgcg gtgtatgtcg atggcatcta ccatggtgtt acgagcacca cagtagggaa
     6901 ccttatcccg ggctcccact cggtcaggtt atccaaggca gggtacttgg actggaccgg
     6961 ctcggtcagc atctcggcgg gctctaccac gtaccttgat ccctccctcg tgaaggatca
     7021 gcagccccag tatgcaacgg tcagcatcac gtcaaacccg gcaggggcga gtgtctacgg
     7081 tgacggggtg tatgtcggcc agacccgtgc cgggagcccc ctcgtcttta cctcggtaaa
     7141 acccgggacc cataccctcc tcctcacgaa gtctgggtat caggactact cggtcacccg
     7201 gaccgtctca gccggacaga actatgatgt cgatgttacc ctgaacccgg tacagaaccc
     7261 gactacgggg ggaatttcga tcatctcggc cccaagccag gccgaggtct acctgaacaa
     7321 tgcctacaag gggcttactc ccgtcaccct ggatagtctc gccccgggat cctacaccgt
     7381 gctgctccgg ctgagcgggt atcaggactg gcaggcgacc cagcaggtga cagcaggcca
     7441 gactgcccag atctcggcga ccctgactcc ctctgcgaca ccaacgccga cccagagcgg
     7501 gcttcttccc ctggtggttc tcgcaggaat tgggatcgtg ttcttagcgc tccggaagag
     7561 atcctgaacc ctttttttct gagaaaggaa aggcattttc ccccagggtc ctggaaatga
     7621 agaattactt tcgctacctg tgatcccgct aggtcacgga tatcacttca gcacgatcat
     7681 cctcctcttc agggatgagg agccaggcga tgaggtaggc gatcactccg acaccgatgg
     7741 aaaggagggt gagcaccacc cagacgatcc ggatcatgtt cggatcgata tcaaaataat
     7801 ggccgatccc actgcatact cccccgagca gccgctc
//
LOCUS       DAOOCO010000084         7717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_8945_length_7717_cov_4.465283, whole genome shotgun sequence.
ACCESSION   DAOOCO010000084 DAOOCO010000000
VERSION     DAOOCO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_7717_cov_4.465283"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PK154_05940"
     CDS             <1..799
                     /locus_tag="PK154_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW10639.1"
                     /translation="EGHEIPEFGENILTARDVYKRYVSVDRGVVKAVNGVSFDVQVKE
                     IFGIIGKSGAGKTTLSRIISGIIEPTSGEMNIRIGDDVVDMTKPGIENRGRAKGYIGL
                     LQEYDLYPHRTVLDNLTDAIGLEFPKELAMRKAILTLKMAGFHEEKSREILDRHPGQL
                     SEGERHRVALAQVLIREPRLIILDEPTGTMDPLTKIDVKHSILHARDEMDETFIVVSH
                     DMDFVKDICDRLALMRGGKIIQIGKTDEVLSSLTEDERQVMGKASSV"
     gene            799..2328
                     /locus_tag="PK154_05945"
     CDS             799..2328
                     /locus_tag="PK154_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HPW10640.1"
                     /translation="MSLTIHLDGKPFEIAEGKLLSEIIPDKDPRCCVAVIRPATRESA
                     STSSFRLVTTQGEITIEPVGGGPEIFSRLTSENLLTIHWHDRYSSAFGPFASTIKPAR
                     SPHLYERGEVILGCGGYDPKHSYIIFSKMRHAADFGADETGGIIGKVVSGIGVLDRWR
                     TGDAIQRIEQVISWADTSRSFTTTDKNLVLEDGMEIITHVLIRAAGYENRRVDTTTAG
                     SVEHLLLSYEELLFHVSRSASTHIADIRKSGTDVPEENRLPRREGSVTARTKGRGRGS
                     IYIYTTDIPASNAHTIVGQVTRGIELVRLAKDGDVFCLKIDPPRFDLVGLLLPAAEQA
                     ASAKGIRLIADRREEERVVVGQTPETTLECLAAGEVHLTTVPLSQVVDISLDDLHSPD
                     SCTVFRRLTGLHLHRVGSLPLFFHFEDVYLFKPAVPKDVTIVPENVPSDTVPAGTLAI
                     TNDSRRGSGLVGVRTTDNSEFGPTSEPFEGTNIIGKVLDLHKLREYKETEIVFIREAG
                     R"
     gene            2325..2783
                     /locus_tag="PK154_05950"
     CDS             2325..2783
                     /locus_tag="PK154_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HPW10641.1"
                     /translation="MTEYQPKYVNTVTKYVFVESPEMTPGDLAIRAYEISQGVMIKET
                     CFGLQITGKEDEVNAIVDHLRKFDPAHIFVKDRGFPPGDSRRCRANLGGARPGYFGHE
                     FEISISRFISKGLVELPKRDALAEPKPPAPDGSAKLDAKKLRKIIESVET"
     gene            2786..3235
                     /locus_tag="PK154_05955"
     CDS             2786..3235
                     /locus_tag="PK154_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HPW10642.1"
                     /translation="MVKVFIYPTTSLILSDLVARFGHTPLSAALQIREHIQTPGLESP
                     PLQITPEDPKKGLKWAAVEVPSGVRGRMSLYGPMLEQAEAAIIIHDADFSFGCMGCAR
                     TNELIQFLLRKRGIPVLDLRYPRTEEEGIEFVAAVKKFLNELAGGGA"
     gene            3232..4467
                     /locus_tag="PK154_05960"
     CDS             3232..4467
                     /locus_tag="PK154_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HPW10643.1"
                     /translation="MNLVRIAQLSCGPEYSGVQREINVAARSVDAEIFFPDIRLADIR
                     EGFDLFGLDVRSPDLKLAIARARALVEGKIEADAVFIATCFRCAEAAIVRNELRRYIH
                     ENSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREVQEGLTLGVDSGSSTTK
                     AVIMRDNQIIGTGWLPTTAVLKSAEDAVDIALKEAGVRREEIQAIGTTGYGRFLVGEK
                     MHADLIQEELTINSKGAVYLADRQHGPSTVIDIGGMDNKAISVQDGIPGTFTMGGICA
                     GASGRFLEMTAKRLGVDITELGPLALRGMGREVPMNSYCIVFGTQSLVNALAAGSSPE
                     DVAAAACHSVAEQVFEQQLQEVDIKEPVIMVGGTSLIQGLVKAMGDLLQVEVVVPHHS
                     QYIGAVGSALLASGFIKGS"
     gene            4470..5051
                     /locus_tag="PK154_05965"
     CDS             4470..5051
                     /locus_tag="PK154_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HPW10644.1"
                     /translation="MDAIEYFEVESPEDAGREYYRRIADVVLLDHNLLRVIKKLHISI
                     DPRIPLFIAVGVTKKIPRVIKVGDIADVNVQERKVTLSIGDETYLAPLLKILWAEYGG
                     DKVVQPDRFTVILNIEGADGRQIEEIPVIDPSEALYKDLIYALLVIQPEGFKVRRQYY
                     GKEKFYLVASENTLPENFEELVKEKFALMGETL"
     gene            5048..5986
                     /locus_tag="PK154_05970"
     CDS             5048..5986
                     /locus_tag="PK154_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HPW10645.1"
                     /translation="MNLVPVTYKGGIYRHDEIMDLIEDLGGYIIQKHVLAQEVVLQAL
                     VPREDIGLIARIGKPLTGEITPSPLVGTEIAVVTMSLEIHHLPHASCDVAEYVRRMGA
                     KTNMVGLARGFGKRIAQLNDEERDVINEHDLAVYLLGDFETCIEHKFPILRRGIHVPI
                     IVLGGPSTETLMRIIDPPVDGYVGNVGRFMHRTKESEELDMLDQVVTEITRVLDKKRE
                     AIAKDPPSVSPARLMDIISSQVDEIHEVLSPTPITVQMTGLRVKLPYDRFAPLLKEIV
                     IEEGITIGEVAEILPSRMRDYILLRIKPFSETNIMV"
     gene            complement(6422..6970)
                     /locus_tag="PK154_05975"
     CDS             complement(6422..6970)
                     /locus_tag="PK154_05975"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HPW10646.1"
                     /translation="MSNSIIPLPRSTWKIVPGILLVVALLLFAGCVGPFEPGPSEIPG
                     EQLTVMPTPTPTVSAEQTSAQTLRNGPAELATSGYIERSYGYVPYSTPPEHRLTVIES
                     NSRKDASGNIIIYGRMKNEGPGSLSYLQVTFNLYDTSGNLLGNAHAHVEYFGAGQTWH
                     FQADPVPATNYQYFKIAGVMAQ"
     gene            complement(7120..>7717)
                     /locus_tag="PK154_05980"
     CDS             complement(7120..>7717)
                     /locus_tag="PK154_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW10647.1"
                     /translation="FGSYKLTGTTIAVGPIGSTKMYCSIPEGCMEQESAYFDLLGMAA
                     AFSFSAGHLHLLDGMGRELLVFSAEKQDLSQESGSFIEYSRTGGFAGFDDHLTIYSNG
                     TASVQRKESERTITIDPGTLSELEILLDTTDFALLNEQYPATQNGADYFRYSLTFGGK
                     TVTTEDTAIPDLLVPVFTILDEIIGSSAPDDVIPPFTG"
BASE COUNT         1951 a         1930 c         2137 g         1699 t
ORIGIN      
        1 cgaaggacat gaaattccgg aattcggcga gaacatcctc accgcgaggg atgtctacaa
       61 gcggtatgtc tcggtcgaca ggggtgttgt caaggcagtg aacggcgtga gtttcgatgt
      121 ccaggtgaaa gagatcttcg gaataatcgg aaaaagcggc gcagggaaga ccacgctttc
      181 acggatcatc tcagggatca tcgaaccaac gtcgggagag atgaatatcc ggatcggcga
      241 tgatgtcgtt gatatgacaa aaccggggat cgagaaccgg gggcgggcca agggatacat
      301 cggcctcctc caggagtacg acctctaccc ccaccggacc gtgctcgaca acctcaccga
      361 tgctatcggg ctagaatttc ccaaggaact cgccatgcga aaggctatcc tcaccctgaa
      421 gatggccggg ttccacgagg agaagagcag ggagatcctc gaccgccatc ccggccagct
      481 ctccgaagga gaacggcacc gggtagccct tgcacaggtg ctcatccggg agcccaggct
      541 gataatcctg gatgagccca ccgggaccat ggatcccctg acaaagatcg atgtcaaaca
      601 ttccatcctc cacgcaaggg atgagatgga cgagacgttc atcgttgtct ctcacgacat
      661 ggacttcgtc aaggatatct gcgaccggct tgccctcatg agggggggta agatcatcca
      721 gattggaaaa acggatgaag tcctgtccag cctcaccgaa gatgaacgcc aggtcatggg
      781 aaaagcttct tcggtataat gtcactcacg atacatctcg atgggaagcc tttcgagatc
      841 gcagagggga aactcctttc ggagatcata ccggataaag atccacgctg ctgtgtcgcc
      901 gttataaggc ctgcaacccg tgaatccgcc agcacctcca gtttccggct cgttaccacg
      961 caaggggaga taaccatcga accagtggga ggcggaccgg aaattttttc caggctcact
     1021 tccgaaaatc tgctcacgat ccattggcat gaccgttatt catccgcttt tggtcctttt
     1081 gcaagcacga tcaagcctgc gaggagccca cacctctatg agaggggaga ggttattctt
     1141 gggtgtggag gatacgatcc caagcattcc tatattatat tttcaaaaat gaggcatgca
     1201 gctgacttcg gagcggatga gacagggggg attatcggaa aggttgtctc cgggatcggc
     1261 gttctggacc gttggaggac cggagatgct atccagagga tcgaacaggt gatcagctgg
     1321 gccgatacca gccgttcatt cacgacaacc gataaaaatc ttgttctcga ggacggcatg
     1381 gagattatca cccatgtctt gatcagggct gcagggtacg aaaaccgccg tgttgatacc
     1441 accactgccg ggagcgtgga acacctgctt ctctcttatg aagagctctt atttcacgtg
     1501 agccggtccg caagcaccca tattgcagat atccgtaaaa gcggtacgga tgtccctgaa
     1561 gagaaccgcc ttcccagacg ggaggggtcc gtaacggcaa ggacaaaagg gagagggaga
     1621 ggtagcattt acatttacac cactgacatc cctgcctcaa atgcccatac cattgtcgga
     1681 caggtaacca ggggcatcga gcttgtacgg cttgcaaagg atggagatgt attctgcctg
     1741 aagattgatc cccctcgttt tgatctcgtt ggtctgttgc tccctgcagc agagcaggcc
     1801 gcatcggcaa agggtataag gcttattgct gacaggcggg aagaggagcg ggttgtggtc
     1861 ggccagaccc cggagaccac ccttgaatgt cttgcagccg gagaagtgca tctcacgaca
     1921 gttcccctgt cgcaggttgt tgatatctcc cttgacgatc tacattcccc tgactcctgc
     1981 actgtcttca gaaggctcac aggccttcac ctgcaccgcg taggaagcct ccccctcttc
     2041 ttccattttg aggatgtgta tctcttcaaa ccagcggtac ccaaagatgt gaccatcgtc
     2101 cctgaaaatg ttccttctga caccgtacca gcagggaccc tcgccattac caacgattcc
     2161 cggaggggtt cggggctggt tggagtcaga acgacggaca atagcgaatt cgggcccaca
     2221 tcggagccct tcgaagggac gaatattatc ggaaaggtcc ttgaccttca taagctgcgc
     2281 gaatacaagg agactgaaat tgtgtttatc agggaggcag ggagatgact gaatatcagc
     2341 caaagtatgt caataccgtc acaaaatatg tatttgttga atcgccggag atgacccctg
     2401 gtgacctggc catcagggct tacgagatct cccagggagt tatgatcaag gagacctgtt
     2461 ttggtctgca gattactgga aaggaggatg aggtgaacgc aattgttgat catctccgga
     2521 aattcgatcc ggcccacatc ttcgtgaaag accggggatt tccgcctggc gattcacggc
     2581 ggtgcagggc aaacctcggg ggtgcacgcc ctggctattt cggccatgaa tttgagatct
     2641 cgatcagtcg gttcatatca aaagggctgg ttgagttgcc aaagagggat gcccttgccg
     2701 aaccaaagcc acctgcaccc gacggaagtg caaagctcga tgcgaagaaa ctccggaaga
     2761 tcatcgaatc agtggagacc tgatcatggt aaaagtattt atttatccta cgacgagtct
     2821 catcctttcc gatctcgtgg cgcggtttgg gcacactcca ctctccgccg cactccagat
     2881 aagggaacac atccagaccc cggggcttga atcccccccg ctccagatca ctccggaaga
     2941 tcccaagaag gggctgaagt gggcggctgt cgaagttcct tccggggtaa gggggaggat
     3001 gtcgttgtac gggccgatgc ttgaacaggc agaggccgcg atcatcatcc acgatgcgga
     3061 tttctctttc ggatgtatgg ggtgtgcaag aacaaacgag ctgattcagt ttctcctgcg
     3121 aaaaagaggt atcccagtcc ttgatctccg atacccaagg acggaagaag agggtatcga
     3181 atttgttgcg gcggtaaaaa aatttctcaa tgaactggcc ggaggtggtg catgaacctt
     3241 gtcaggatag cacagctctc atgcgggccg gagtactcag gggtccagag agagatcaac
     3301 gtggcggcca gatctgtcga tgcagagatc tttttcccgg acatcaggct tgcagatatc
     3361 agggagggtt tcgacctttt cggcctcgat gtccgaagtc ctgatttgaa actggcgatt
     3421 gcccgggcac gggcactggt tgaagggaag attgaggctg acgctgtgtt tattgcaaca
     3481 tgtttccggt gtgctgaagc tgcgatagtg agaaatgaac tccgccggta catccatgaa
     3541 aattcccgcc ttccggttgt cagctactca tttactgagc ggaccactgc agggacactc
     3601 ctcaccagga tggaagcact tacaacaatt gcgaggagaa gggcgctgct tgcccgagaa
     3661 gtccaagaag gactgacgct tggtgttgat tccggctcaa gcacaacgaa agcggtgatc
     3721 atgcgcgata accaaatcat cggtacgggg tggcttccca cgactgcagt actgaaaagt
     3781 gccgaggacg ccgtcgatat cgctctgaag gaggctgggg tgagacggga ggagatacaa
     3841 gccatcggaa caaccggata cggacgcttc ctcgttggag agaagatgca tgccgatctc
     3901 atccaggaag aactcacgat caactccaag ggagccgtct acctcgccga caggcagcat
     3961 ggcccatcaa ccgtgataga tatcggaggg atggacaaca aggcaatcag cgtgcaggat
     4021 gggataccgg gaacattcac gatgggtggg atctgcgccg gggcaagtgg caggttcctc
     4081 gagatgaccg ccaagcgact tggggttgat atcaccgagc tcggccccct tgcccttcgt
     4141 ggcatgggca gggaggtccc gatgaacagc tactgtattg tgttcggcac ccagagtctg
     4201 gtgaatgccc tcgcagcagg cagttctccg gaggatgttg ccgcagctgc atgccatagc
     4261 gtcgcagagc aggtattcga acaacagctc caggaggtcg acatcaaaga accggtgatc
     4321 atggtgggag ggacatcact aatccagggg ctggtgaaag ccatgggtga cctgctccag
     4381 gttgaggtgg ttgtccccca ccactcccag tatatcgggg ctgtggggtc tgcactcctt
     4441 gcatcagggt ttatcaaggg atcgtaagaa tggatgcgat cgaatatttc gaggtggaga
     4501 gccccgaaga cgcaggtcgt gagtattatc gccgtatcgc agatgtggtg ctccttgacc
     4561 acaaccttct ccgggttata aaaaaactac atatctccat cgatccgcgg atccccctct
     4621 tcatcgcggt gggggtgacc aaaaaaatcc cgcgggtgat taaggtaggg gatattgctg
     4681 atgtgaacgt ccaggagagg aaggtgacac tctcgattgg ggatgagaca taccttgcac
     4741 cgctcctgaa gatactctgg gcagagtacg gtggagacaa ggtggtacag cctgacaggt
     4801 tcacggttat cctcaatata gagggggcag atggtcgcca gattgaggag atacccgtca
     4861 tcgatccaag cgaagcgctc tacaaggacc tgatctacgc gttgctggtt atccagcccg
     4921 aagggttcaa ggtacggcgg caatactatg gcaaagagaa gttttacctc gtggcaagcg
     4981 agaataccct ccctgaaaac ttcgaggagc tggtgaagga aaaatttgca ctgatggggg
     5041 agaccttgtg aaccttgtac cggtaaccta caaagggggg atctaccgcc atgacgagat
     5101 catggacctg atcgaagacc ttggtggata catcatccag aaacatgtcc ttgcccagga
     5161 agtagtcctt caggcgcttg ttccacggga agatatcgga ctcatcgcac ggataggaaa
     5221 gccgcttacc ggggagatca ccccttcacc gcttgttggg accgagatcg cagtagtcac
     5281 gatgagcctc gagatccatc acctccccca cgcctcctgt gatgtcgctg aatatgtgcg
     5341 gcggatgggt gcaaagacca acatggtcgg gcttgcacga ggcttcggga aaaggattgc
     5401 acaactgaac gatgaggagc gggacgtcat caatgagcac gaccttgctg tctacctcct
     5461 cggtgacttc gagacatgta ttgaacataa gttccccatc ctccggagag gtatccatgt
     5521 acccatcata gtgctcggcg gaccgtccac cgagacactg atgaggatca tcgatccgcc
     5581 tgtagacgga tatgtcggga atgtcggccg tttcatgcac aggacgaagg aatcagagga
     5641 actcgatatg ctcgatcagg tcgtgaccga gatcacacga gtactcgaca agaagaggga
     5701 agcgattgcc aaggatccgc catcagtgtc acccgcccga ctgatggaca tcatcagcag
     5761 tcaggtcgat gagatccatg aagtcctctc accaactccc atcactgtgc agatgacagg
     5821 tcttcgggtg aaattgccgt atgatcgatt cgcccccctg ttgaaagaga tcgttattga
     5881 agaagggatc acaatcggcg aggttgcaga gattctcccc tcaaggatgc gtgactatat
     5941 tctactacgg ataaagccat tctctgaaac aaatatcatg gtctgattgt gggtattgag
     6001 agggagttct acctcatcct tctctggtag atcaggatta ggttgatttt tttctgtctg
     6061 attgaccatt ttcaccagag gacttgagag aaaactctga aaatatgcca ttttcgcagt
     6121 tccacaatcg attttaaaaa aaattaaatc ccatttaaaa tctacaaaga atgagaaatt
     6181 tgatgtatca cacgcaatcg cattgtgggg gacaccgcag cagcggggga taaggccgaa
     6241 ggcgcaaccc ccaggaggga tatgatagac gtgagatcct ttatgagggc aaaaaatgat
     6301 ggatgaaatg ccaccaggtc ctcttccgat caacttcgga atactccatt gagatttcac
     6361 gaaaataccg acagaagatc ctccttcctc cctgattgac cgggtaaaaa aatgaacggg
     6421 tctattgtgc catgaccccg gcaatcttga aatactggta gttggttgcc ggaacaggat
     6481 ccgcctggaa atgccaggtc tggcctgcgc cgaaatactc tacatgggca tgagcatttc
     6541 cgagcaggtt cccactggta tcatagagat tgaaagtaac ctggagataa gagagggaac
     6601 ctggcccttc attcttcatc ctgccgtaaa tgatgatatt tcctgatgca tccttccggg
     6661 agtttgattc aatcacagtg agacggtgct caggaggtgt tgaatacggc acatagccgt
     6721 atgatcgttc gatatacccg gacgttgcca gttcagcggg tccgttgcgc aaggtctggg
     6781 cagaggtctg ttcagctgat accgtcggag tgggggtagg cattactgtc agctgttcac
     6841 caggaatttc tgaggggccg ggttcaaacg gcccaacaca gcccgcaaaa agaagaagcg
     6901 ctaccacgag cagaattccg ggcacgatct tccatgtact acgggggaga gggatgatgg
     6961 aattactcat ggattccctg tggatttttc agggataagt accttttgag ggagagatat
     7021 tccccggaac cacaacaact gtcaggggaa tcctgacggt gtggttctga tccctcagct
     7081 gcctgaagca tcagagggac tatccacccc caaaaccatt tacccggtaa aaggtggaat
     7141 cacatcatcg ggtgcgctgc tcccgataat ctcatccaga atagtaaaaa ccggaacaag
     7201 gagatcggga atcgctgtat cttccgttgt aacggtcttt cccccaaagg taagggagta
     7261 cctgaagtaa tcagcaccgt tctgcgttgc gggatattgt tcgtttaata gtgcgaaatc
     7321 tgttgtatcc agcaaaattt caagttcgga cagtgttccc ggatcgatcg ttatggttcg
     7381 ttcagactcc ttgcgttgta ccgatgcggt tccattggaa taaattgtca ggtgatcatc
     7441 gaatccggca aatcctcctg tacgggaata ttcgataaag gatcccgatt cctgagacag
     7501 atcctgcttt tctgctgaaa agaccaggag ctcccggccc attccatcga ggagatgaag
     7561 atgtcccgca ctgaaagaga atgcagccgc cataccgagg agatcaaaat aagcgctctc
     7621 ctgctccatg cagccctcgg gtattgagca gtacattttt gtggatccga ttggtcctac
     7681 tgcaatcgta gtccccgtca gcttatacga tccaaag
//
LOCUS       DAOOCO010000085         7619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9099_length_7619_cov_4.790719, whole genome shotgun sequence.
ACCESSION   DAOOCO010000085 DAOOCO010000000
VERSION     DAOOCO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9099_length_7619_cov_4.790719"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..2006)
                     /locus_tag="PK154_05985"
     CDS             complement(339..2006)
                     /locus_tag="PK154_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10648.1"
                     /translation="MRYLLFTALALVLLVGAASALPSEVTQTIVATASGGDLSQEAYQ
                     SAEVVGEGAVLTQGIEVSAEGTGEVAQDADNYGYADGAAAVVTQGITMEATGENVYQN
                     PFTSGNYAEVFGEGAILGQYMALAAAAEEYLYQYAYNDAWTIGDGIVTTQGIAQAGAA
                     GGDINQYAYNDASVWGIGTTTTQGIALAATSIDGWISQDVWNDADPDAGSLADQEIAL
                     AAATGGDIDQDAYNYAYFYGDGAVALQAVSMDADAGGYADQYGYNYADMGDYDTIMVD
                     QSATLNAVAGTWVWQEFYNDNEYAGSNTVSDQAITMSGTAGTWIDQYMGNLFWDWGTV
                     SDVSQVLTAAAYAGDGNAYQDAGNGLISYGDDIASQSIFLDAVATEYVTQYGYNYADS
                     LDNLDLGQEVWLGGEGAYVYQEADNEAFFGDAVDVAQYIALTGISDDEGVNQFADNNA
                     ENMYALGGSSNMIGQAITGAATGGDYSFQYFENDCDAEYNLLAPSSYTITQSNIAILA
                     ANDEAYQELYNGIDWYAWNTGSIGWNINGYASADFVYQYHENWVEII"
     gene            complement(2542..3072)
                     /locus_tag="PK154_05990"
     CDS             complement(2542..3072)
                     /locus_tag="PK154_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10649.1"
                     /translation="MNRKIVLALVLGLCLLCSGVSALPVITATISANVSGQNFSQYAV
                     NSVVSQGGPVSVTQTIDLSSVGAGSLFQNAINRVEGAGTGSQIRQGISLSGLAPLQVQ
                     NQQNYANVAGDPLTHVTQENRASGGAGGSSLSQFQKNEIFWANGIRGVPFQNNYATGT
                     ASFLERIQQNMEFNLA"
     gene            complement(3162..4298)
                     /locus_tag="PK154_05995"
     CDS             complement(3162..4298)
                     /locus_tag="PK154_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW10650.1"
                     /translation="MHIAYVYDAIYPYILGGVERRVWELSQRLAKRGHEVHLYGMQWW
                     DGEADIEREGVMIHGTCRPVPLYRGGKRTVSQAVRFGASMARPLVRERFDVIDCQQFP
                     YTSALVAVLAARISRSPLVITWHEVWGDYWYEYLGAAGAAGKLAERLLARRSDHPVAV
                     SETTRAGLVSAGVETDVRIIPNGIDLAEIDRIRPSGSGSDLVFIGRLIREKHVDLLIR
                     AVSILKEREPGISCLIIGDGPERALLEGLVASCGVRENVCFTGFLPDSGEVIALMKSS
                     RVFVLPSTREGFGIAALEALASGLPVVTIDHPGNASREFASGGCGRLARLDPGDLARV
                     IGEVLSSLGNNRDTCQLRASSYDWDAVTDRIEDYYIDIISGSVQ"
     gene            complement(4311..4922)
                     /locus_tag="PK154_06000"
     CDS             complement(4311..4922)
                     /locus_tag="PK154_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10651.1"
                     /translation="MEFASEMLIKASREGLTITEVPITYHPRGTPSKLSSYSDGWRHL
                     RFILLLRPMPFLTVPGILCTLFGLVLMAAFYTSANDPDGRTHSFILGTILLTGGFQLL
                     LSGVVIKVYSAVHGFERREGMVEELLNYQTLEKLLVAGSVLVLAGLIIGGYILWGWMV
                     AGFGSLNQIRNAVLSLSLVIIGLEIVFIAVFISMMCLNGDNPS"
     gene            complement(4898..5461)
                     /locus_tag="PK154_06005"
     CDS             complement(4898..5461)
                     /locus_tag="PK154_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW10652.1"
                     /translation="MESDNNTTSPDISVILPALNEETTIGICIGKIQQMFRSMGIRGE
                     VIVSDSSTDATPEIAQSMGAIIVHPEHRGYGYAYLEAFRYARGRYIVIGDADNTYDFL
                     ELPLLIRKLDEGADLVIGSRFKGEILDGAMIPLHRYIGNPLLTWVLNKVFGTDFSDTH
                     SGFRAIRADSLKRLNLQSWEWNLPPRC"
     gene            complement(5650..>7619)
                     /locus_tag="PK154_06010"
     CDS             complement(5650..>7619)
                     /locus_tag="PK154_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HPW10653.1"
                     /translation="LPSALTGISLGLGMAVMPTLILFGLIVVLYSTIQYCWNAFHGKR
                     SDYLLLINGAVSLGSIAGLAAVGIHSPVYSLSTYSAAPFHAFIALFCWTLLLQIFSMA
                     TRGKPWYFIGLLGSSIVAVIGVAAILDPSLFSSWTGSFSAFFGQNFSATPIDELKPWS
                     LGQMWASFNIGILLGIVGLALFAYSFWKKECPLYLFSLVWGIVLVFATIQHSRYEYYS
                     AVIIVIAATFALATAFSLDKSSKSHHPPARQKNDPKGKKEKKRAKEVKKNTNEGLFAA
                     LEGTGTSLVLACMIVFCGFSVLADYSIAVPMTKDDLIPQQWSGVLGWVAASSPDPGVP
                     YLGSYGEGDQWRYPEESYGVLSWWDYGHWITVISRRIPVTNPFQDNLVPSAAYFFADS
                     EETANAIADNYRVTYVITDWKMVESKFPAMVAFYNSSLGERSLPADYYYQVFRLASPD
                     KKGNQTLVRLHASPFYHTMVSRLHSFDGSMAKPQTVLYVEYDIPASARTTPGISVYEN
                     LDPGTAREKIAVFNATPHERRKAAILNTGLDAPVETIPALRHYRLIFEEAGTGNQTSP
                     SYTQAVKAFEYVKGARLKGDGEIEVTIQTNLGRTFVYRQQSENGFFILPYSTKGSPYP
                     VHTTGPYRSVSTGRTFEVSEQEVLEGRVIAG"
BASE COUNT         1879 a         2087 c         1925 g         1728 t
ORIGIN      
        1 gctgtgaata cgagatatct catttgtttt catcactcct gtaaagttgc aagagatgaa
       61 tccccccaac aatacagaag atactagagt aattcgcgat ttaaatgtat cgaatagttt
      121 tcagggaacc tgcctgtgcg gggtgatgtc gtaatttatc accaatgttc ggttcaaaag
      181 atgacaagct gagatgtgac gaacgctgtc gcatgtgtcc aatgccggct gcgaagtcca
      241 tctgttttca gtcacaaccc tgatagtgcc cggaaaaaaa tgcctttgaa ttatgggatt
      301 ttcatcgtaa tcaagaaaaa atgagagatc cgggaagatc agataatctc aacccagttc
      361 tcgtgatact ggtacacaaa gtcagcggat gcatatccgt tgatattcca tccgatcgag
      421 ccggtgttcc aggcgtacca gtcgatacca ttgtagagct cctggtatgc ctcgtcattc
      481 gcggccagaa ttgcaatgtt gctctgggtg atcgtgtagc tggatggagc gaggagatta
      541 tactcagcat cgcaatcgtt ctcgaaatac tggaatgagt aatccccgcc agtcgctgca
      601 cccgtaatgg cctggccgat catattcgat gatccgccaa gggcatacat gttctcagca
      661 ttgttatccg caaactggtt gacaccttcg tcatcggaaa tgccagtaag agcgatatac
      721 tgtgcgacat ctacggcgtc gccaaagaag gcctcgttat ctgcttcctg atatacgtaa
      781 gctccttcgc caccgagcca gacttcctgg ccgagatcga ggttgtcaag actgtcggca
      841 tagttgtagc catactgggt cacatattcg gttgccacag cgtcaaggaa gattgactgg
      901 ctggcgatat catcaccata ggagatcagt ccattgcctg catcctggta ggcattgccg
      961 tcccctgcat acgcggccgc agtcaatacc tgggagacat cggaaaccgt tccccagtcc
     1021 cagaataaat tgcccatgta ctgatcgatc caggttcccg cagttccgct catggtgatg
     1081 gcctggtccg acaccgtgtt gcttccggcg tactcgttgt cgttgtagaa ttcctgccag
     1141 acccacgtgc cggcgactgc attgagcgtt gcagactgat cgaccatgat cgtgtcataa
     1201 tcgcccatgt cggcgtagtt gtagccatac tgatcggcat aaccaccggc atccgcgtcc
     1261 atgctcaccg cctgcaatgc tactgccccg tcaccgtaga agtaggcata gttgtaagca
     1321 tcctggtcga tatcgccgcc ggtcgctgct gcaagggcta tctcctggtc tgccagggac
     1381 ccggcatcgg gatcggcatc attccagaca tcctgggaga tccaaccatc gatggaagtc
     1441 gctgcaaggg ctatcccctg ggtggtagta gtgccgattc cccatacaga ggcgtcattg
     1501 taggcatact ggttaatgtc cccgccagcc gctcctgcct gggcgatacc ctgcgtagtg
     1561 actatgccat caccgatagt ccaggcatca ttgtaggcat actgatacag atactcttca
     1621 gcagcggcag caagcgccat gtactggcca aggatcgctc cctcgccaaa tacctcggca
     1681 tagttaccag aggtaaacgg gttctggtac acattctccc cggttgcctc catggtgata
     1741 ccctgggtca ccaccgcggc tgccccatct gcatacccat agttatcggc atcctgtgcg
     1801 acctctcccg taccttcagc agagacttca ataccctggg tcagtactgc accctccccg
     1861 acaacctccg cggattggta ggcttcctgg gataggtcgc caccgctcgc cgttgcaacg
     1921 atggtctggg taacttcgct tggcagggca gacgctgccc ccaccagcag cacaagtgcc
     1981 agagcggtaa ataaaagata tctcatctgg tttcatcact cctgtaaagt tgtaagagat
     2041 gaattccccc aacgatacaa tggaacttgt ctctcttatg gttttaaaga ttttgaaaga
     2101 cgttgaaaag gcaggtgaac tggtccatcc tggattaccg gtcatgtgcc ataggtctgt
     2161 catgtaccac aggtggagcc ggcccaggta tctggtagca ttcacatttc attcccggtt
     2221 cccgagtcca gattgtcgtt ctcgcctgat cttatctatt gagttagcga ttgtactaac
     2281 cgtccgacag ggctctgaaa ggtgatttct ttttcaaaat gcaatatgag aaggaaaatc
     2341 aatgtgatac tgaaatctca aaaggaaaca aacccattat aaggtctgca tcaccgtcca
     2401 ggtatccgat acgaatccat tcaccatccg gaagaactca agggtgagaa aagagtccct
     2461 gaatcaaaca gtacatgaat ctggttgaga tgcgggtatg taccacgaaa atggcgaagc
     2521 gagtttgaaa aataaaaaat attacgccag gttgaactcc atgttctgct gtattcgttc
     2581 gaggaacgaa gcggtgccgg ttgcataatt gttctgaaac ggcacccccc tgattccgtt
     2641 tgcccagaaa atttcattct tctggaactg ggagagcgaa ctcccccctg caccacccga
     2701 tgcccggttc tcctgagtga catgggtgag aggatcacca gcgacattgg cataattctg
     2761 ttgattctgc acctggaggg gagccaggcc ggataaggag atcccctgcc gtatctggct
     2821 cccggtgccg gctccttcaa ccctgttgat cgcgttctgg aacagtgacc ctgctccaac
     2881 agatgacaga tcaatagtct gggtaaccga caccggcccg ccctggctga caaccgagtt
     2941 gacggcatac tggctgaagt tctgccctga tacattcgcc gatatggtgg ccgtaattac
     3001 cggcagtgcg gataccccag agcagagcag gcacaatccc aggacgagag cgagcactat
     3061 tttccgattc atgcgaatcc atcctgctaa tacttagctg tcactgcatc ctactgatac
     3121 ttattatttt ttgatcgcaa tagttcaaga aaagaacagg gttattgaac acttcctgat
     3181 atgatatcga tataataatc ctcgatccgg tcggtaactg catcccagtc ataggaggat
     3241 gcccgcaact ggcaggtatc ccggttgttc cctaaagaag agagcacttc tccgattact
     3301 cgcgcaagat cccccggatc gagcctcgca agcctcccac accctcctga agcaaattcc
     3361 ctgctcgcat tcccgggatg atcgatggtc acgaccggaa ggccactggc gagcgcttcc
     3421 agtgcggcaa taccaaatcc ctcgcgggtt gacgggagga caaacacgcg ggacgacttc
     3481 atgagggcaa ttacttctcc tgaatccggt aaaaatccgg taaaacagac attctcccgg
     3541 actccgcatg atgccaccaa tccttccagc agagcccgct cgggtccgtc accgataatc
     3601 aggcagctga tacccggctc cctctccttc aggatgctca ccgcacggat aaggaggtca
     3661 acatgcttct cccggatcag tcttccaatg aagaccaggt cagaaccaga tcctgaagga
     3721 cggatacggt cgatctccgc gagatcaatg ccattgggga taatccggac atctgtctcc
     3781 actccagccg acacaagccc tgcccgtgtt gtttctgata ccgcaacagg atgatcactc
     3841 ctccgggcaa gaagccgttc tgccagcttc cctgcagccc ccgcagcccc aagatactcg
     3901 taccagtagt ccccccacac ctcgtgccac gtgatgacca gcggcgatcg ggagatccgg
     3961 gcagcgagca ctgctacgag ggctgatgtg tacgggaact gctggcaatc gataacatcg
     4021 aatcgctccc tcacgagagg gcgtgccatg ctggccccaa atctgaccgc ctgggagacg
     4081 gttcttttcc cgccacggta cagcgggacc ggcctgcagg tcccatgaat catcacccct
     4141 tccctctcga tgtccgcctc gccatcccac cactgcattc catagagatg gacctcatgc
     4201 ccgcgtttcg caagccgttg cgatagctcc catacccgtc gttcgacccc gccgaggatg
     4261 taggggtata tcgcatcata gacatacgca atatgcatcg gatccccccg ctatgacgga
     4321 ttgtcaccgt tcaggcacat catgctgatg aataccgcga tgaaaacgat ctcgagcccg
     4381 atgatgacga gcgagagtga cagtacagca ttcctgatct ggttgaggga cccaaatccc
     4441 gccaccatcc atccccacag gatatatcct ccgatgatca gcccggcaag caccagcacc
     4501 gatccggcga ccagtaattt ttcaagggtc tggtagttga gcagttcctc gaccatcccc
     4561 tcccgccgtt caaacccatg caccgctgag tagaccttga tgaccacacc agagagcagg
     4621 agctggaatc ccccggtaag gaggatggtc ccgagaataa acgaatgggt ccgcccgtcc
     4681 ggatcatttg ccgaggtata gaacgctgcc atcaatacaa ggccgaacag ggtgcagagg
     4741 atcccgggaa cggtaaggaa tggcataggt ctcaacagaa ggatgaagcg gaggtgtctc
     4801 cacccatcac tgtaactgct cagcttggag ggagttcccc ttggatggta ggtgattgga
     4861 acctcggtga tcgtcagccc ttcacgtgaa gccttgatca gcatctcgga ggcaaattcc
     4921 attcccagct ctgcaggttc agccttttta aggaatctgc ccgtatggca cgaaatccgc
     4981 tgtgcgtgtc ggaaaaatca gtaccgaaga ccttgttgag gacccaggta aggagaggat
     5041 ttccgatata gcggtggaga gggatcatcg cgccgtcgag gatctccccc ttgaaccggg
     5101 agccgattac gaggtcggcg ccttcatcga gcttcctgat caggagggga agttccagaa
     5161 agtcataggt attgtctgca tccccgataa cgatatacct tccacgggca taccggaatg
     5221 cctccagata ggcgtaccca tatccccggt gttcgggatg cacgatgatg gcgcccatcg
     5281 attgggctat ctctggggtc gcatcggttg atgaatcgga gacaattacc tctcccctga
     5341 tccccatcga acggaacatc tgctgaatct tcccgatgca aatcccgatg gtggtctcct
     5401 cgttgagggc cggcaggatg acggaaatgt caggagaggt cgtattgttg tctgattcca
     5461 cggtgagtca cctgccacct cgagcgggaa ctattgacca gagatcgaac agcaagaaac
     5521 ctgttcggcc ggagatcgca taatgattcc tatatgaatt atccttttct ccgcaaaaaa
     5581 tgccagcaga ttccagatga gaccctccct tgtccattga aaagaaagaa cagggatcat
     5641 atcccggttt caccctgcaa tgaccctgcc ttcgaggact tcctgttcgg aaacctcaaa
     5701 cgtcctcccg gtagagacac tccggtaagg cccagtcgtg tggaccgggt aaggagatcc
     5761 ttttgtcgaa tagggcagga tgaagaatcc gttctcgctc tgctggcgat agacgaaggt
     5821 cctgccgagg ttggtctgga tggtgacttc gatctcgcca tcccccttca atcgtgctcc
     5881 ctttacatat tcgaatgctt tcactgcctg ggtgtatgag ggactcgttt gattccctgt
     5941 gcccgcctct tcgaatatca ggcgatagtg acggagggca gggattgttt caaccggtgc
     6001 atcgagtccg gtattgagga tggcggcctt ccgtctctcg tgaggagttg cgttgaacac
     6061 ggcaattttt tcacgggccg ttcccggatc aagattctcg tagaccgata tacctggcgt
     6121 ggtcctcgcg cttgccggaa tgtcatattc cacgtacaat accgtttgcg gtttggccat
     6181 cgaaccatcg aaactgtgga gcctgctcac catggtatgg tagaatggtg atgcatgtaa
     6241 cctgacaagg gtctggttcc cttttttatc aggagatgca agacgaaata cctgatagta
     6301 ataatcggcc ggaagggatc gctccccaag agaactgttg taaaatgcta ccattgcagg
     6361 gaattttgac tcgaccatct tccagtcggt tatcacatat gtaacccggt agttatcggc
     6421 gatagcgttg gcggtctcct cagaatcagc aaagaaatac gctgcagagg ggacgagatt
     6481 atcctggaag ggattggtaa ccgggatgcg ccgggatatc acggtgatcc agtgcccgta
     6541 atcccaccat gacagcaccc cgtaagactc ttcaggatag cgccactggt ccccttcccc
     6601 ataggatccc agatagggca caccagggtc gggactcgac gcagcaaccc atccaagcac
     6661 accactccac tgctgtggga tcagatcatc ttttgtcatg ggcactgcaa tggaataatc
     6721 cgcaagaaca gaaaagccac aaaagacgat catacatgcc agcaccaatg aggttccggt
     6781 cccctcaaga gcggcaaaca atccttcatt tgtattcttt ttgacttctt tcgctctctt
     6841 cttttccttt ttgccttttg ggtcgttttt ttgtctggca ggagggtggt gcgattttga
     6901 cgatttgtca agagagaacg cagttgcaag cgcaaacgtc gcggcgataa caatgatcac
     6961 ggcactgtaa tattcatacc gggaatgctg gatggtcgca aatacaagca cgatccccca
     7021 cacaagggag aagagataca gcgggcattc cttcttccag aatgagtaag caaataaggc
     7081 caatccgaca atccccagca ggataccgat attaaaactc gcccacatct ggcccagcga
     7141 ccagggcttc agctcatcaa tcggagtagc cgaaaaattc tgtccaaaga atgctgagaa
     7201 cgaacctgtc cacgatgaga agagagaggg atccaatatg gctgccacac caatgaccgc
     7261 cacaatggag gaacctaaaa gcccgataaa ataccagggt ttgcctctcg ttgccatcga
     7321 aaagatctgg agcaggaggg tccagcagaa gagggcgata aatgcatgga aaggcgctgc
     7381 agaataggtg gagaggctat agactggcga atggatgcca accgctgcca gaccggcgat
     7441 ggatcccagg gataccgctc cattgatgag aaggagatag tccgatctct tcccatgaaa
     7501 tgcattccag cagtactgta tggtagaata gagcaccacg ataagaccaa aaaggatgag
     7561 ggtaggcatg accgccatac caaggcccag ggaaattccc gttaatgctg aagggagaa
//
LOCUS       DAOOCO010000086         7577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9153_length_7577_cov_5.020340, whole genome shotgun sequence.
ACCESSION   DAOOCO010000086 DAOOCO010000000
VERSION     DAOOCO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_7577_cov_5.020340"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..2034)
                     /gene="glgB"
                     /locus_tag="PK154_06015"
     CDS             complement(100..2034)
                     /gene="glgB"
                     /locus_tag="PK154_06015"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPW10654.1"
                     /translation="MTGVGTAGEPESPFLSDYDIFLFRQGRHYRLYQKLGSHPLVLKE
                     GAGTWFAVWAPNAQRVTVIGDFNGWDPASHPLRERPDGSGIWEGFIAGAVEGMRYKYH
                     IISRYYNHTADRGDPFAFAWELPPATASLIAEPCIGWQDRAWMEERKNRQDTTSPISI
                     YEMHLGSWRHVPSDGGRAPTYRELAPMLAGYLAEMEFTHVEFLPVMEHPFYGSWGYQT
                     IGYFAPTSRYGTPGDFMYLIDHLHRNGVGVILDWVPSHFPSDPHGLGFYDGTHLYEHA
                     SPQRGFHPEWKSLIFNYGRHEVQSFLISSALFWLDRYHADGLRVDGVASMLYLDYARK
                     VGEWIPNQYGGKENLEALSFLQNLNEAVYGTFPDIQTFAEESTAWPMVTRPKYTGGVG
                     FGLKWNLGWMHDTLRFFSRDSLYRSFHLPELTFSICYAFSEHYVLCLSHDEVVHGKGS
                     LFDRMPGDDWQKRANLRLLFGYLYTHPGKKLLFMGDEFGQRSEWDHQDSLSWQLLESA
                     EHRGIRSWIRDLNRVYREENALHRTDSDPLGFEWVDFGDTRNVAFSFLRKDRESGEFL
                     LAICNFTPIPRQDYRIGLPAGGEWTELLNSDAGHYGGSGIGNLGSVKADPVPFHDRPF
                     SAAFTLPPLAFLLLKHRGGE"
     gene            complement(2031..3191)
                     /locus_tag="PK154_06020"
     CDS             complement(2031..3191)
                     /locus_tag="PK154_06020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW10655.1"
                     /translation="MPRLKIAHLCWESLHAVREGGLSPAATHLAEVLSGTHEVHFFTR
                     GDGPDRTIGGVFYHFCQPEGEGILQYCRDMSRQILDKFRDADNPPFDIIHFHDWHCSD
                     ALEELKERTTVMSYHSTEYGRSGNNFGNSLEFLAIAAKEWRAGHTARHVTTVSNHTKT
                     EIMWLYGIPERNITVIPNGIYPDTYFLPLDAGQVKKRYGIHPLAPLVLFIGRLVYQKG
                     ADLLVEAIPQVLKRRWDVQFIIAGKGDMRQELEERCRTLPVRFTGYISDEEYRELLNA
                     CDLVVIPSRNEPFGLVLTEAWSAERCVVATDVGGLSENIDDFIDGIKVPVRADSLAWA
                     ISYIIEDPVRIRAMGLAGKRKVLSRFNWGEIGTKMERVYMNLIADRQKEARS"
     gene            complement(3173..4600)
                     /locus_tag="PK154_06025"
     CDS             complement(3173..4600)
                     /locus_tag="PK154_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10656.1"
                     /translation="MGLNIHYPYFVETGAHATDRPRECSTCFSRSQRTRFREYLSQAA
                     LPAMEMSLSWLDAGEPLSINVSGVAIEQFLGDENDAADILVQLLRHKAVAILGQTYYR
                     SVAGLFSETGEFISQVSRHAELMEGLSGKRPEVFENTEFVFNSELTNAIRALGFAALY
                     SEADESILPVLNPNYVYSCNNLPVLIRNCRLSDDIAHRFGDVTWDRYPLTPEKYAGWI
                     AETPGDCIHVRVDAGIFSGAAGGARALGQFFHGLPEALRSREITPILPLSAARNHPRA
                     EIRLEDLGLVSSDRAYALTGIRTILQQSAFWCLEDARYLIPDQESWRRFQSTDHFSRM
                     DTISGSCGRVVIRSTSQAAYDYFSAYLIALARCEASCREDLWSPAAAQVLRCLPPEKA
                     FHFHSGHRFIGYSAHSLKEFSRLLEFIPEEVFSFHQERGDFSRWIDEVLGDSTLAERI
                     MHCATPHEAAELARERGRELCHDLK"
     gene            4851..5132
                     /locus_tag="PK154_06030"
     CDS             4851..5132
                     /locus_tag="PK154_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10657.1"
                     /translation="MWFELLVLLMGTAFGYFRSGKEDIRSIIRQGLIYGILLGIVLGI
                     LSIFAASGMSIDAGVQGAIGLSVRIIILVILFIIGIMIGDGIEMYRKNH"
     gene            complement(5266..6186)
                     /locus_tag="PK154_06035"
     CDS             complement(5266..6186)
                     /locus_tag="PK154_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPW10658.1"
                     /translation="MTRRILLVSCLLMFALVCAGCLSAPWDSDSVKIDEQFAPSTSGS
                     GIPSRDVPYPIPTIVPTYAPGYAADLEADQKIIRTGNLNLEVKNVSSTLDPLKQIATL
                     HGGYLGSMSVNTQYGNRLYASLTIRIPAGEFDATVSDIKKLGTLKSESLSADDVTEEY
                     VDLQARRNALASQLVQYNRIMEKAENVSEILEVQVQIERVQVELDRIDGRLKYLDNRV
                     DYGTITVSLVEPEPVGQGKEFSLVSVLNDGIQGFLAVTGGLIIILISIIPLIILAIVL
                     YLIYRWWRKRKGEKRSTSEAKKKGDNPPGV"
     gene            complement(6622..7425)
                     /locus_tag="PK154_06040"
     CDS             complement(6622..7425)
                     /locus_tag="PK154_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HPW10659.1"
                     /translation="MTSRMNFFLLFIAISCILTAGCITPETTPENSPVTAPTTESSPV
                     PTTEPPTPSDTVPEAAQFTRDDVYELFMDIAFGCDNTGVTMFPPSSENHLFFSLEGQV
                     IPADIGFVSLFTREFNRITPIEVFTDEPLNSRGSPIIFYPDDSLDSLEKRFVACQELD
                     PESGKPLYIIYKSVTELPNGESETTTKIYLNAHLAGAEREHYLQKAMLYYLGFPGQTY
                     NYPDSVFYYNTRSTTDFSPIDIEAMKTLYHPGIYEGMSIQEVRWLFFND"
BASE COUNT         1706 a         2010 c         2162 g         1699 t
ORIGIN      
        1 cagggtgcag ctgaaggctc tttttcgacc atccccgtcc acggattttc cctgggaggc
       61 tggtaaaagt gtccatggat acagatcttc tttcccatgt tattcacctc ccctgtgttt
      121 caggagcagg aacgcaagcg gcgggagggt gaatgccgca gagaacggtc tgtcgtggaa
      181 ggggacagga tcggctttca ctgatccaag attccctatc cccgatcctc cgtaatgtcc
      241 tgcatcactg ttcaggagct cggtccactc tccgccggca ggaaggccga tccggtagtc
      301 ctgccgtgga ataggagtaa aattgcagat ggcgaggagg aactcgccgg attccctgtc
      361 ttttctgagg aaactgaatg caacattcct ggtatcaccg aaatcgaccc attcaaatcc
      421 cagcgggtca ctatcagtcc ggtgaagggc attctcctcg cgatataccc tgttcaggtc
      481 cctgatccag gaccggattc ccctgtgttc tgccgactcg aggagctgcc aggagaggct
      541 gtcctggtga tcccattctg accgctgtcc gaactcatcc cccatgaaga gcagtttctt
      601 tccaggatgg gtatagagat acccgaagag cagccgcagg ttggcccttt tttgccagtc
      661 gtcgcctggc atccggtcga acagtgatcc cttcccatgg actacctcgt cgtgggaaag
      721 gcagagcaca taatgttcag agaaggcata gcagatcgag aaggtcagct cggggaggtg
      781 aaacgagcgg tacaggctgt cacgggagaa gaaccggagg gtatcatgca tccatccaag
      841 gttccacttc agcccgaagc cgaccccccc ggtatatttc gggcgagtca ccatcggcca
      901 ggcggtcgac tcctcggcga aggtctggat atcggggaag gtaccatata ccgcctcatt
      961 gagattctgg agaaaggaaa gtgcctcgag gttctccttc cccccgtact ggttggggat
     1021 ccattccccc actttcctgg cataatcgag gtagagcatc gaggccaccc catccacccg
     1081 gagaccgtct gcgtggtacc gatccagcca gaaaagtgcg ctgctgatga gaaacgactg
     1141 cacctcatgc cgaccgtaat tgaagatcag gctcttccat tcagggtgaa atccccgctg
     1201 gggagaggca tgctcgtaca ggtgggtccc gtcatagaat cccaggccgt gcgggtcaga
     1261 gggaaaatgg gaggggaccc agtccagaat gacgccgacg ccgttcctgt ggaggtgatc
     1321 gatcaggtac atgaaatcgc caggggtgcc gtaccggctg gtcggggcaa aatacccgat
     1381 ggtctggtat ccccaggatc cgtagaaggg gtgctccatg accgggagga actccacgtg
     1441 cgtaaattcc atctctgcaa gatacccggc aagcatcggt gcaagttccc gatatgtcgg
     1501 agcccgccct ccatcagacg ggacatgccg ccatgatcca agatgcattt cataaatgga
     1561 gatcggcgag gtggtatcct gccggttctt ccgctcctcc atccatgccc ggtcctgcca
     1621 tccgatacac ggttctgcaa tgagggaggc ggtcgcaggg gggagctccc aggcgaatgc
     1681 gaacggatca ccccggtctg ccgtatgatt gtagtagcgg gagatgatgt gatacttgta
     1741 ccgcatccct tcaaccgctc cggcaatgaa gccctcccag atccctgaac cgtcaggtcg
     1801 ttcccgcagc gggtgggagg cagggtccca tccattgaaa tccccaatca cggtcacccg
     1861 ctgggcatta ggggcccaga ccgcaaacca cgtgcccgct ccctccttca ggacaagagg
     1921 atggctgccc aatttctgat agaggcgata atgccttccc tgccggaaga ggaagatatc
     1981 atagtcagag aggaacggcg attccggctc tccagcggtg cccaccccag tcatgatcgt
     2041 gcctctttct gccggtctgc tatcaggttc atgtaaaccc gctccatctt tgtgccgatc
     2101 tctccccagt tgaaccggga aaggaccttt cgttttccgg caagtcccat tgcccggata
     2161 cggaccggat cttcaatgat gtaactgatc gcccaggcaa gtgaatcagc cctgacgggg
     2221 accttgatac catcaatgaa atcatcgata ttctccgaga gtccccccac atcagttgcc
     2281 accacgcatc gctcggcact ccatgcctcg gtgagcacca gcccgaatgg ttcgttccgg
     2341 ctggggatga cgacaaggtc acacgcattc aggagctcgc ggtattcctc gtcagagatg
     2401 tatcctgtga agcggacggg gagcgtcctg cagcgttcct caagctcctg ccgcatatcc
     2461 ccttttccgg caatgatgaa ctggacatcc cagcgtcgtt tcaggacctg ggggatggct
     2521 tccaccagga ggtcggcacc cttctggtag accagtcggc caatgaagag tacgaggggg
     2581 gcgagtggat ggataccgta ccgttttttc acctggccgg catcaagcgg gaggaaatag
     2641 gtatcagggt atatgccgtt tggaatcacc gtgatgttcc gctccgggat gccatacagc
     2701 cacatgatct cggtcttggt atggttcgag acggtggtca cgtgccgtgc cgtatggcct
     2761 gccctccact cctttgccgc aatggcaagg aactcgaggc tgttcccgaa attgttcccg
     2821 ctccgaccgt attcggtgga atggtagctc atcacggtgg ttctctcctt taattcttcg
     2881 agtgcatcgc tacaatgcca gtcatggaaa tggatgatat caaacggggg attgtccgcg
     2941 tctcggaact tgtcgaggat ctgcctgctc atgtcacggc agtactggag gataccctcc
     3001 ccttcaggct ggcagaagtg gtagaagacc cccccgatgg tccggtcggg cccgtctccc
     3061 ctggtgaaga aatgcacttc atgggttccc gagagcacct ctgcaaggtg cgtcgcggca
     3121 ggggagagtc ccccctcacg gacagcatgc agggactccc agcagagatg ggctatttta
     3181 agtcgtggca tagttccctt cccctctccc tggccagttc tgccgcttca tggggtgttg
     3241 cacaatgcat gatgcgttca gcaagcgtgc tatctccgag gacctcatcg atccagcggg
     3301 aaaaatcacc ccgctcctgg tgaaacgaga atacctcttc agggataaat tcgagcaggc
     3361 gtgaaaattc tttcaggctg tgggccgaat acccgatgaa ccggtggccc gaatggaaat
     3421 ggaaggcctt ctcggggggc aggcatcgga gaacctgtgc agcggccggt gaccagagat
     3481 cttccctgca ggaagcctca cagcgggcaa gagcaatcag gtatgctgaa aagtagtcat
     3541 acgccgcctg gctcgttgac cggatcacga ctcgcccaca cgatcctgaa atagtgtcca
     3601 tgcgcgagaa atggtcggtg ctctggaacc tccgccagga ttcctgatca gggatcaggt
     3661 agcgggcgtc ctcgaggcac cagaacgctg actgctggag gatggtccgg ataccagtca
     3721 gggcatacgc ccgatcggaa gatacaagac cgagatcttc cagccggatc tctgccctgg
     3781 ggtggttccg ggctgccgat aacgggagga tgggggtgat ctcccgtgaa cggagggctt
     3841 ccggcagacc atggaagaac tgccccagtg cccgggctcc accagctgct cccgaaaata
     3901 ttcctgcatc tacacggacg tgtatgcagt cgcccggggt ttctgcgatc caccccgcat
     3961 atttttccgg ggtcagtgga taccggtccc acgtgacgtc cccgaaccgg tgggcgatat
     4021 catcagaaag acgacaattc ctgatcagga cgggcagatt attgcaggaa tagacatagt
     4081 tcggattaag gacagggagg atcgattcat ccgcctcgga ataaagggcg gcaaaaccga
     4141 gtgccctgat agcgttggta agttcagagt tgaacacgaa ctcggtattt tcaaagacct
     4201 ccggcctctt cccagaaagc ccctccatca attctgcatg cctgctgacc tgacttatga
     4261 attcaccggt ttccgagaag agaccagcaa cgctccggta gtaggtctgc ccgagtatcg
     4321 cgactgcctt gtggcggagc agttgcacca ggatatcggc cgcgtcgttc tcgtctccga
     4381 gaaactgctc gattgcgaca cctgaaacat tgatcgagag gggttcccct gcatccagcc
     4441 aggagaggct catctccatg gcaggaagag cagcctgcga gaggtactcc ctgaaacggg
     4501 ttctctgtga tcgggaaaaa caggtgctgc actccctggg acgatcggtt gcatgggcgc
     4561 cagtctctac aaagtaggga taatgaatgt tcagtcccat gcagaagtgc cgacatcctc
     4621 tcccgttcat tatgagtgat gattactagt tgggagtatt taattgaggc agatggggaa
     4681 cgttcagggg gattgttctt ccttttcagt agctttccgg gcggtacgca cacgcatgtc
     4741 ctgttatgaa tctctcttcg attggaaccg tttcatcccg attcggattt ccctacttca
     4801 ccagggggta taaacaaggg aagggagata ctatacccag gataaaggtc atgtggttcg
     4861 aactcctggt cctgctgatg ggaactgctt ttgggtattt ccggagcgga aaagaagaca
     4921 tcaggagcat catcaggcag ggattgatct atgggatcct actcgggatc gtgctcggga
     4981 tcctttcaat ttttgccgct tccgggatga gtatcgatgc aggggtgcag ggagcaatcg
     5041 gcctctcggt caggatcatc atcctggtga tcctgttcat catcgggatc atgatcggag
     5101 acggtatcga gatgtacaga aaaaatcatt aatgagggag ttgggcagac ccccggttga
     5161 tggaaagggt ccacacgttc accctaaact cccttatatc ctctcgtgac atcccgggag
     5221 aaagatggct ggaggtattt ccatccctgg agaggggcct ccggactata ctccaggagg
     5281 attgtcccct ttcttctttg cttcggaggt cgatctcttc tcgccttttc gctttctcca
     5341 ccagcggtag atcaggtaca gtacgatcgc caggatgatc agagggatga tggatatgag
     5401 gatgatgatc aaccccccgg taactgcaag gaatccctgt atcccgtcgt tgagcaccga
     5461 gacaagagag aattctttgc cctgtcctac cggttcaggc tcaacgagcg ataccgtgat
     5521 cgtgccatag tcaacccggt tgtccagata cttgagacgg ccgtcgatcc tatccagctc
     5581 gacctgcacc cgttcgatct ggacctggac ctcgaggatc tcggagacat tctctgcctt
     5641 ttccatgatc cggttgtact ggacgagctg gctggcgagg gcgttcctcc gtgcctggag
     5701 atcgacatat tcctcggtaa catcatccgc actgagcgat tcggatttga gtgtcccgag
     5761 cttcttaatg tcacttaccg tcgcgtcgaa ttcacccgca gggatcctga ttgtcaggga
     5821 tgcatacagg cggttcccat actgggtatt caccgacatg gatccgagat acccgccatg
     5881 cagggtggcg atctgtttga gagggtcaag cgtggaagag acgttcttga cttcgagatt
     5941 cagattgccg gtcctgatga tcttctggtc ggcttccagg tcagcagcat atcccggggc
     6001 atatgtagga acaatggtgg ggatcggata agggacatcc cgggaaggga ttcctgagcc
     6061 tgacgtggaa ggggcgaatt gttcgtcaat cttcacacta tctgagtccc agggcgctga
     6121 aaggcacccg gcacagacta aagcgaacat gagaaggcag ctcacgagga gaatgcgacg
     6181 agtcatatca agaacatttt cataaaagat aataaataat ttgtaaactg cgattttttt
     6241 cgcaaccatc ctggtcaacc cctgaatcct ggacgatgcc tggataaagg gggaagtctt
     6301 tgcggctgcc ggaatgcaac gatcgattct ctgctcacca tcaccctgct cttctccgcg
     6361 caattgcaag ccttgatgag gtaaggaacc atattcacat tttttccctg ggaagacact
     6421 gtcagcttgg gatggacagg ggaggagatc aggagaaaag gatgccaggg gaaggggtta
     6481 ccaggagaaa agaaaggcgg gttctgcttc attgcataca cagatccagg gttgctcata
     6541 ctcagtgaat gttcaatgag aatcgaagaa agaggactgg cctggatgct tctccccagg
     6601 aaatgagatg acaggagggg gttaatcgtt gaagaagagc caccgcactt cctggatcga
     6661 catcccctcg taaatcccgg gatggtacag ggtcttcatc gcctcgatgt cgatcgggct
     6721 gaagtcggtc gtcgaccggg tgttatagta aaagacactg tcgggatagt tataggtctg
     6781 gcccgggaat ccgaggtaat agagcatcgc cttctgcagg taatgttccc gttccgctcc
     6841 ggcaaggtgg gcgttgaggt atattttcgt ggtggtttcg ctctccccat tcggtagctc
     6901 ggtcacgctc ttataaatga tgtaaagggg ttttccggac tctggatcga gctcctggca
     6961 ggcaacgaag cgcttttcaa gagagtccag ggagtcatca gggtaaaaga taatggggct
     7021 cccccggctg ttgagcggtt catcggtgaa tacctcaatt ggcgtgatgc ggttgaactc
     7081 cctggtgaac agggagacaa agccgatgtc tgcgggaatc acctgccctt caagcgagaa
     7141 gaaaaggtga ttttcggatg agggagggaa catggtcacc ccggtattgt cacagccgaa
     7201 tgcaatgtcc atgaagagtt cgtagacgtc gtccctggtg aactgggctg cctcgggtac
     7261 tgtatcggaa ggtgttggcg gttcagttgt gggaaccggg ctgctctctg ttgtcggagc
     7321 ggtgacaggg gaattctctg gggttgtctc gggggtgata caccctgctg tcaggataca
     7381 tgaaatggca atgaacagca aaaaaaagtt catccttgat gtcatgaata aaggtaccct
     7441 catcccaata aatagatgcc cgccatggat tctctcggaa aagaggatgg tgatgcggga
     7501 agcaggagcg atatacccct gtatagcagg gagagggttt catcctataa tggggctttc
     7561 cgctttttcc aatgaaa
//
LOCUS       DAOOCO010000087         7484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9291_length_7484_cov_5.307040, whole genome shotgun sequence.
ACCESSION   DAOOCO010000087 DAOOCO010000000
VERSION     DAOOCO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_7484_cov_5.307040"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..792)
                     /locus_tag="PK154_06045"
     CDS             complement(178..792)
                     /locus_tag="PK154_06045"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPW10660.1"
                     /translation="MEKMYTLPKLPYDYRSLAPYISEDQLTLHHTKHHQAYVTGANAL
                     LEKFDKARKEKTDFDVKATSKELSFHIGGNRLHALFWKNLAPAGNGGGGLPKGDLAKA
                     IDAEFGGFDRFRREFSTAATSVEGSGWAALSYCNESGRLLMMQIEKHNMNTFPGYPLL
                     MVLDVWEHAYYLDYRNDRAKFVEAFWNIVNWDEVGKRLAGAVKK"
     gene            complement(884..2608)
                     /locus_tag="PK154_06050"
     CDS             complement(884..2608)
                     /locus_tag="PK154_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPW10661.1"
                     /translation="MHKETRFYSGLKLIALILCIAGIVIPSAIAAETVVEDIADLPNT
                     TQTSSPTQAIQGEDLTTVTTNGSVPDTQPLDFSAPPQIVLMPARVMDLTSTILGTTIP
                     GSVNATITSIQWDWGDNTTTEQHEFPHSHTYRSPGSYTLTITAFQSDGLNTTRATTIT
                     IDEPVAIPTRDPLIISIPGIPTNQPGPANGPALTLLEPVIDGMNVTVNGNLNAGSPGV
                     TISSVRIDWDEGNVTNVTDLPATYRYAAAGIYTITITAAQSDGQTISKGITVDITAEN
                     PGPPGLPAVSTPPPYGQPLYLIILTTAVIVILALVIGQRLFQKRREDPRAADIPEAVS
                     LQEKIYYEAKGKGDLTTAAASAHICAQMFRALAEKSPEKRGLYLDLAEKWETIAATTG
                     KTGAPEPHPQKSGTVAEKIPSRRELEAICDGTDVSPEVVESVIQVAMEIGREGREGQA
                     VGTSFVVGDTDAVMTNSRQFVLNPFQGHNEAERQITESGIKGNIKEFAQLDGAFIISG
                     DGIVESAGRYITVDMSQVKVPDGLGSRHSSIAGITLVTSSIGIVVSQSGGLITIFKNG
                     TIVHTIGS"
     gene            2768..3172
                     /locus_tag="PK154_06055"
     CDS             2768..3172
                     /locus_tag="PK154_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10662.1"
                     /translation="MDLSEYLAAHPEKSRELKSATGCRCENCSLEYPLALLEVHTIGS
                     TLSVETPHIDLQYYLLVLCPSCSRSFRSGKVALSLQRDLVRLRNRQVRRRMRAILSYQ
                     PKPYTPSVDFDPEVIFREVVSSTSPGLCLNGG"
     gene            3293..4138
                     /locus_tag="PK154_06060"
     CDS             3293..4138
                     /locus_tag="PK154_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW10663.1"
                     /translation="MVDSIKKRFILLVLLTALALCCSPAIAESAGTLTVTGDDLVDEG
                     KYEEAISYYDQAIALEPRMAMAWTGKGVALNELGRYAEAKSALEEAIKLTPNYAKAWY
                     QKGIALNGLTQYEEAIVAFDTALSVYPEYGYLAYYGKANALAGLGKYTDAIQWYDKAL
                     GLESRYAEPWTNKGNAHAALGDYQNALAAYEKALSIEPNYLPAKNGLNAATANLSAGM
                     PATQGVPQQTGTTAMVTTTPMATTVVQTTMQSTTPQTTQGLALPLWLTLPGIAFAVYL
                     SKRRT"
     gene            complement(4292..4762)
                     /locus_tag="PK154_06065"
     CDS             complement(4292..4762)
                     /locus_tag="PK154_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10664.1"
                     /translation="MALKENFSSDEWENLVGLPYAISMTIMVAAPNVMGVWGETKATM
                     QEPAKLAAKSGSALVGLLLAEMQPRLQELMKKQQAALKQNQAGYRANTIEACRRAAAA
                     LSKVPPEEALAYKNWVLGIGLKVAEAAKEHGVAVSDPEKVVIAEISSALGIPAQ"
     gene            4936..5118
                     /locus_tag="PK154_06070"
     CDS             4936..5118
                     /locus_tag="PK154_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPW10665.1"
                     /translation="MATDPVCYMVVNEDETEWRIHYKGQEFYFCSDFCRKKFEQNPGR
                     YAKLARSMDIGPDISC"
     gene            5257..6744
                     /gene="pap"
                     /locus_tag="PK154_06075"
     CDS             5257..6744
                     /gene="pap"
                     /locus_tag="PK154_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HPW10666.1"
                     /translation="MFEKCDLSKKIDDKTFEKEIKPLQVEIGVLTRALWEHRVPIIIV
                     VEGWNASGITMVISELIQHIDPRGFSLHSVGSPNDEERARPLLWRFFTRIPAKGRIAI
                     FARSWYSRSLAEQVSGIDWKSAVRFSIDTLNSFERQLADDGAVIIKFFLHISKEEQKR
                     RLDEREGNLLTSWMITKGDWDFHHHYDSYLPVIELFIEGTDKSYAPWTIVEATDSNHT
                     CLKVYSTIVKRLRKHLDRIEINGKPEGKNNAKNGIIKPPKTTVKRQSAPQVEYSRPEY
                     EKHLVRCQEKVRDIQYLLYKRRIPLIIVYEGWDAAGKGGNIMRLVRLMNPRGYDVVSI
                     SRPDQTELDHHYLWRFYQRFPKAGHITIFDRSWYGRLMVERVEGFCTASEWRRAYREI
                     NEMEQAYVGSGGGLIKFWLEVSKDEQLERFQDRQNDPLKEWKITDEDWRNRTKWNEYE
                     EAVDEMLGRTSTAEAPWTIVESDDKYYARLKTLQTVIEYGKQLFR"
     gene            complement(6778..7140)
                     /locus_tag="PK154_06080"
     CDS             complement(6778..7140)
                     /locus_tag="PK154_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10667.1"
                     /translation="MDPQSPHALRVIECLVELQRQMGGNAMIGRELWHLLNDAGFSRV
                     AVSPRQVYADSSRPEYIEGVRNIFISMVKGVRDQALAADLIDADSWERGIRDLYRTTE
                     KDGSFAYTFFKATGVKSR"
     gene            complement(7199..7417)
                     /locus_tag="PK154_06085"
                     /pseudo
     CDS             complement(7199..7417)
                     /locus_tag="PK154_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         1743 a         1976 c         1972 g         1793 t
ORIGIN      
        1 agatgcgata ggatggtatc aaccgcatta ttccttcatt gtgaattgct ctatgcgatt
       61 tacctttttt tcatcgattt ttggaactgc aaacatggaa tatttttaga cgtaatgtaa
      121 tcgcaggatt ggtggtaaaa aatagtacta tacccaaaaa aaatcccaaa aaaagaatta
      181 ctttttcacg gcaccagcca gtctctttcc gacctcgtcc cagttgacga tgttccagaa
      241 tgcttccacg aacttggccc tgtcgttccg gtagtcaagg taatatgcat gctcccacac
      301 atccaggacc atcaggaggg ggtacccggg aaatgtgttc atgttgtgtt tctcaatctg
      361 catcatcagc agccttccgc tctcgttgca gtaactgagt gccgcccagc ccgatccctc
      421 aacactcgtt gctgcggtgg agaactccct cctgaaccgg tcaaatccac cgaactcggc
      481 atcgatcgcc tttgccaggt cccctttggg cagccctccg cccccgtttc ctgccggtgc
      541 caggttcttc cagaacaggg catggagcct gttgccgccg atatggaacg agagctcctt
      601 tgaggtcgcc ttgacatcaa aatcggtctt ctctttgcgg gccttgtcga atttttctaa
      661 gagggcattt gcccctgtca cataggcctg gtgatgcttc gtgtgatgca gcgtcagctg
      721 atcttcagaa atatacggtg caagtgaccg gtagtcgtag ggaagttttg gaagggtgta
      781 cattttttcc atgaatgttt ctctcctctg ataaatgaca gtggaggagg ttatcctatt
      841 tgtagtattg gatggaggaa aggcatcctc tgattatcag gattcacgac ccgatcgtat
      901 gcacgatcgt gccattcttg aaaatggtga tcaggccgcc gctctgggag acgacaatcc
      961 cgatggagct ggtcaccagg gtgataccag cgatagacga gtggcgtgat ccaagcccgt
     1021 ccgggacttt cacctggctc atgtcaacgg taatgtaccg tccggcagac tcgacaatcc
     1081 cgtctcccga gatgatgaat gcgccatcga gctgggcaaa ttccttgata ttccccttga
     1141 tcccgctctc ggttatctgc cgttctgcct cattatgacc ctggaacggg ttcaggacaa
     1201 actgccggga attggtcatc accgcatcgg tatctccgac cacaaacgag gtcccgactg
     1261 cctgcccctc tctcccctcc ctgccgatct ccattgccac ctggatgacc gattcaacaa
     1321 cttccggact cacatcggtc ccatcacaga tcgcttcgag ttcccgtcgg gaaggtattt
     1381 tttcagcgac agtaccagat ttttgcggat gaggttcagg ggcaccagtc ttccccgtcg
     1441 tcgcagcaat agtctcccac ttctctgcca gatccaggta gaggccccgt ttttcgggag
     1501 acttctctgc cagggcacgg aacatctgcg cacagatatg ggcgcttgct gcggcggtgg
     1561 tcaggtctcc cttccctttt gcctcgtaat atatcttctc ctgcagggag actgcctccg
     1621 ggatgtcagc cgcccgtgga tcttcccttc tcttctggaa caatcgctga cctatcacca
     1681 atgctaaaat tacaatcacg gctgtcgtga ggataatcag atacagcggc tgtccatatg
     1741 ggggaggagt tgagaccgca gggagtcccg gaggaccggg attttccgct gttatatcaa
     1801 cggtgatccc tttggaaatc gtctgtccat ccgattgtgc agccgtgata gtgatcgtgt
     1861 agatgcctgc tgcagcatac cggtaggttg cgggaaggtc ggtcacattc gtgacattcc
     1921 cctcgtccca gtcaatcctt accgatgaga tggtaacacc cggtgatccg gcattgagat
     1981 tcccgttcac ggtcacgttc atgccatcga tcaccggctc aagcagggtg agtgcgggtc
     2041 cgttggccgg accaggttga ttggtgggga tacccggaat cgaaataatc aacggatccc
     2101 gggtagggat cgcaactggt tcatcgatgg taatagtggt ggcccgggtg gtattcagcc
     2161 catctgactg gaatgccgtg atggtgaggg tatagctgcc ggggctgcga taggtgtggg
     2221 aatgagggaa ttcgtgctgt tcggttgtgg tgttatcccc ccagtcccat tgaatgcttg
     2281 ttatggtggc attgacggat cccggaatag tagtgcctaa aatggtgctg gtaaggtcca
     2341 tgactcttgc cggcatgagc acgatctggg gaggggcgga aaaatccagt ggctgtgtgt
     2401 caggaacact gccattggtg gtaaccgttg tcaggtcctc tccctgaatg gcctgagtgg
     2461 gagaggacgt ctgggtcgta ttcgggagat cagcgatatc ttccacgacg gtttctgcag
     2521 cgatggcaga tgggataacg atacccgcaa tacatagtat caaggcgatc agcttcagtc
     2581 ctgagtaaaa acgggtttcc ttgtgcatgg ctgccccagg ccgtatcgta taagctatcg
     2641 tggtgattgt tcttaaattt ctggaatgct tcacctgaat gaagccctca ttgcggttca
     2701 ggggattagg atgccaggga gcaggaaaca atttatcatc ccacgatcat ggaacgttac
     2761 ttggatcatg gacctgtcag aatatcttgc ggcacacccg gaaaagtcaa gggagctgaa
     2821 gagtgccacg ggatgcagat gtgagaattg cagcctggaa tatcccctcg cccttcttga
     2881 agttcacacg ataggttcaa ctctttcagt ggagaccccc catatcgatc tccagtacta
     2941 tctcctcgtg ctctgcccat catgctcccg ttcctttcgc tccggcaagg tcgccctatc
     3001 gttgcagagg gacctggtcc gtctgcggaa ccgtcaggtc aggagaagga tgagggcgat
     3061 cctctcgtat caaccgaaac catatacccc ctccgtcgac tttgatcccg aagtcatctt
     3121 tcgggaggtg gtcagcagca ccagccctgg cctgtgcctg aatggggggt aactgttttt
     3181 tgtgttttgt ggaagaataa aaatgaatcc actattaagg agaggaatat ttttcatctc
     3241 ccactccaag gttcactcat tggcaatttg atacgtgcca aggggaattt tcatggttga
     3301 tagcattaaa aaaagattca ttctcctcgt gctcctcacc gcgcttgctc tctgctgttc
     3361 accggcaatc gcagaatctg caggcaccct cactgttacg ggtgatgatc ttgttgatga
     3421 aggaaaatac gaagaagcga tcagctatta tgatcaggct atcgccctcg aaccaaggat
     3481 ggccatggcc tggacgggga agggggtagc gttgaatgaa ctgggacgat atgccgaagc
     3541 caagagtgcc ctcgaagagg ccataaagct cacccctaat tatgcgaaag catggtatca
     3601 gaaagggatt gcactcaatg gtctcaccca atatgaggaa gctatcgttg cgtttgatac
     3661 cgctctctcc gtgtatccgg aatatgggta tcttgcatat tacggaaagg caaacgctct
     3721 tgcaggcctg ggcaaataca ccgatgcaat tcagtggtat gacaaagccc ttggtcttga
     3781 gtcccggtat gctgaaccat ggacaaataa gggcaacgcc catgccgcac tcggggatta
     3841 tcagaatgct ctcgctgcgt atgagaaggc actctcaata gaaccgaatt atctcccggc
     3901 caagaacgga ctgaatgctg ctactgccaa cctttcggca ggaatgccag caacccaagg
     3961 ggtaccgcaa caaaccggta ccaccgcgat ggtcacgact actccgatgg ccacgactgt
     4021 cgttcagaca acgatgcaga gcaccactcc ccaaaccacc cagggactcg ctcttccact
     4081 atggctgaca ctcccgggta ttgcctttgc agtatacctc tcaaagcgaa gaacctgata
     4141 ggcttgcatg aactcatggt ggcgcttagt gagccacctc acactctttt ttgtgccgga
     4201 ggcatcatca tattaaaaaa aggaatggct tttcaggaac atcagaggtt cctgaggtcc
     4261 tcctcctttt tttaatgttc aggattgatt ttcattgtgc agggataccc agcgccgatg
     4321 atatctcggc aataaccacc ttttcgggat cgctgacggc aaccccgtgc tcctttgccg
     4381 cctcggctac cttgaggccg atcccgagaa cccagttctt gtacgccagc gcctcttcgg
     4441 gagggacctt tgacagcgcc gctgcagccc tcctgcaggc ttcaatcgtg ttcgcccggt
     4501 agccggcctg gttctgtttg agggcggcct gctgcttctt catgagctcc tgcagcctcg
     4561 gctgcatctc ggccaggagg agcccgacca gtgcactccc gctcttggct gccagctttg
     4621 caggttcctg catcgttgct tttgtctcac cccatacccc cattacgttg ggggcggcaa
     4681 ccatgatggt catcgagatt gcatagggaa gaccaacaag attctcccat tcatcgctgg
     4741 aaaagttctc cttcagtgcc atagagaatc tcctgtattt cattgaactt aaaggatctt
     4801 tttcatcccg caataggggg agaaaaatcc tttttgggag gaattgcgtc atcccgcatc
     4861 cgcacacatg acatgggtaa aagcattttt atagttcttc ctgtataatg caccagctgc
     4921 caggaggttg gaccgatggc aacagacccg gtctgctata tggtcgtcaa tgaggatgaa
     4981 acggaatgga gaatccatta caaggggcag gagttctatt tctgctctga tttctgcagg
     5041 aagaaatttg aacagaatcc cggccgttat gcaaaacttg cccgatcaat ggatatcggg
     5101 ccggatatca gttgttaacc tgcccttatt ttttctctgg atcatcaggc cttcctgttc
     5161 aattctgtac ctttgactcc ccagggacta cggaatccgc attcccaacc ttaaagtctc
     5221 ctccagtaca tccccttctt atatcctgga gaggcgatgt tcgaaaagtg tgacctgtcg
     5281 aaaaagatcg atgataaaac atttgaaaag gagataaaac ccctccaggt tgagatcggg
     5341 gtcctcacgc gagcactgtg ggaacaccgg gtgccgatca tcatcgttgt tgaaggctgg
     5401 aatgcatccg ggatcacgat ggtgatcagc gagctgatcc agcacatcga tccccgcggg
     5461 ttctccctgc attcagtcgg gtcgccaaac gacgaggaac gggctcgtcc gctcttgtgg
     5521 cggttcttta ccaggatccc ggcgaaaggc aggatagcga tctttgcacg gagctggtac
     5581 tcccggtccc ttgccgagca ggtatccgga atcgactgga aatcagcggt acggttctcg
     5641 attgacaccc tcaacagttt tgagcggcaa ctcgctgacg atggcgctgt catcatcaag
     5701 ttcttccttc atatcagcaa ggaggagcag aagcggcggc tcgatgaacg ggagggaaac
     5761 cttctcacct cctggatgat caccaaaggc gactgggatt ttcaccatca ttacgacagc
     5821 tatctgccgg tcattgaact gtttatcgag ggaaccgata agtcctatgc cccctggacc
     5881 attgtcgaag cgaccgacag caaccacacc tgcctgaagg tctactcaac catcgtcaaa
     5941 cgtctgagga agcatctcga ccggatcgaa ataaatggaa agccggaagg gaagaacaac
     6001 gcaaagaacg gtatcataaa acccccgaaa acgacggtga aaagacagtc tgcccctcag
     6061 gttgagtatt cacggccgga atatgagaag cacctcgtca ggtgccagga aaaagtcagg
     6121 gacatccagt acctcctcta taagcggagg atccctctca ttatcgtata cgagggctgg
     6181 gatgcagcag ggaagggggg gaacatcatg cggctcgtcc gtctgatgaa tccccgtggc
     6241 tatgatgtcg tgtcgatctc ccggcctgac cagaccgagc tcgatcatca ttacctctgg
     6301 cgtttctacc agcgcttccc gaaagccggg catatcacca tctttgaccg gagctggtat
     6361 gggcgtctca tggtcgaacg ggtggaagga ttctgtacag cttccgagtg gagaagggcc
     6421 taccgggaga tcaatgagat ggagcaggca tatgtgggga gcgggggagg actgatcaag
     6481 ttctggcttg aggtgagcaa agatgaacag ctcgaacggt tccaggaccg tcagaatgat
     6541 ccgctgaaag agtggaagat cacggatgag gactggagga acaggacaaa atggaacgaa
     6601 tacgaggagg cagtagacga gatgctcgga cggacgagca cagcggaagc tccctggacg
     6661 attgtcgagt cagatgacaa atattatgcc cggttaaaga ccctccagac cgtgatcgaa
     6721 tacgggaagc agctgttcag gtgaataggg ggtcagggac atcgtgcaga ccacgattca
     6781 cctggatttc acaccggttg ccttgaagaa ggtataggca aatgatccat ccttctctgt
     6841 ggtccggtag agatcccgaa ttcctctttc ccaggaatct gcatcgataa ggtcggcagc
     6901 aagagcctgg tccctcactc ccttgaccat agagatgaag atgttcctca ctccttcgat
     6961 atactccggc cggcttgagt cggcatacac ctgccgtggt gataccgcta ccctcgaaaa
     7021 tcccgcgtcg ttcaggaggt gccagagctc cctcccgatc atcgcattcc ctcccatctg
     7081 ccgctggagt tcgacaaggc attctatcac ccggagggca tgcgggcttt gaggatccat
     7141 ggtccccctc aatcacggta atcgtccctc ctgccttgag cagatttttc atctctttca
     7201 gggcacgggg tggatcaggg aggtgctcga ggaggaaaca gagaaagatg tgatcaaagg
     7261 acgcaggggg gaaagggaga tgatagatat ccccctgctg gaaaaccaca ttttctatgt
     7321 gttcttccct gatacgccgc tcggcaagac ggagggattc tgatgagata tccacggaga
     7381 caaactgtgc accgggactg ttctttgcca ggatcacggt ctgcccaccg acaccgcatg
     7441 ctgcctcaag cactctgctc cccggcaggt agcgggtatc ccaa
//
LOCUS       DAOOCO010000088         7424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9373_length_7424_cov_5.677568, whole genome shotgun sequence.
ACCESSION   DAOOCO010000088 DAOOCO010000000
VERSION     DAOOCO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_7424_cov_5.677568"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK154_06090"
     CDS             <1..595
                     /locus_tag="PK154_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW10668.1"
                     /translation="TVTVDAPIGQVACIMIERDISGVPVVDRAGITGIVTKSDLMKST
                     SVGSLTKRVEEAMEDVITVSRYHSLDHVIDLMRERNDKVVVVNNDGSLAGIVTESNLA
                     FFLYSNVRADLPVKDITRLRKEESGGKKVLRSVYETAVVAEDLMSHPVMTIPPDALIG
                     EAVSVMRDQSVNSLIVVADQEIKGIIKRDDIIREVAQ"
     gene            592..1368
                     /locus_tag="PK154_06095"
     CDS             592..1368
                     /locus_tag="PK154_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW10669.1"
                     /translation="MTEELFVKDVMSRPVTIAKAAVITEALDKMLNEGIDPLIVMNNN
                     SVVGTVSRWSVAEKLGTKRNSSISPNSIHVANTVEEDFTTVYPDQDVEILIPLLQTYK
                     IVVVYDEDHRLIGKVTAEDLLKVYRPNGTLEEVMEEACTIDTEERVVHLRRRMIDDKI
                     IRFIVTDQDRVVGIVTETDVAVAMRNFREVVDDKHQDHRIRNLLVKDIMSKPVLSVSF
                     GSTVDQVINLMVAKKISSVPVIRGETLAGLVTRDSLIRAL"
     gene            1450..2409
                     /locus_tag="PK154_06100"
     CDS             1450..2409
                     /locus_tag="PK154_06100"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPW10670.1"
                     /translation="MKPRTPVQPTNDVSRLLKGMSATGFQGRKLGESLDIWAQMCQDP
                     DCTILLGLSGAMIPAGMQQCLIELVENRYVDVIVSTGANIFHDVCEHYGISHYLGHHQ
                     ADDCHLFSKGIDRIYDVFAYEEQFRSIDLKFADFAGSIAPFSCSSSEFIRKFGRWMIS
                     QHPEQRSLLSECVKMNVPVFIPALCDSSIGIGLMVARRNGVEAYIDQIADVDEIARIV
                     EGSKKTGVIYIGGGVPKNFIQQTQVIADMYDAGQEGHAYAIQYTTDAPHWGGLSGCTF
                     EEAISWGKEAPHAPRVQCFCDATIALPFITSGLIARKIRRTKN"
     gene            2574..2658
                     /locus_tag="PK154_06105"
     tRNA            2574..2658
                     /locus_tag="PK154_06105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2608..2610,aa:Ser,seq:gct)
     gene            2692..3144
                     /locus_tag="PK154_06110"
     CDS             2692..3144
                     /locus_tag="PK154_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPW10671.1"
                     /translation="MAQEEDIKYFVRISNTDLDGTKPVHIALTGIRGVGMHTAQIIAS
                     LAKVDEKETMGRLKDEEVDRIREIADTYTQRVPFWMMNRPKDVYTGESKHLLGTDVST
                     ARDEDINLLRKIRCYRGIRHETGQKVRGQRTKSTGRTGLTVGVKKKKE"
     gene            3154..3699
                     /locus_tag="PK154_06115"
     CDS             3154..3699
                     /locus_tag="PK154_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPW10672.1"
                     /translation="MGYPGKNHKQYQTPKRPFEKSRIEEETKLAIEYGLRNKREVWKA
                     QSYLRRYRKAARELLALMSTGTDRALFDAKKDELIGHMQRAGLLGPDADIDNVLSLKV
                     QQGLERRLQTLVYRQGLARSPKQARQLITHGHIAIDGKRCSIPGYRVKRAEESKISYY
                     GKSPLAASEHAEKARITKQGR"
     gene            3703..4098
                     /locus_tag="PK154_06120"
     CDS             3703..4098
                     /locus_tag="PK154_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPW10673.1"
                     /translation="MAGEKEKWGIAHIYASFNNTIITVTDLSGAETVTKSSGGMVVKQ
                     DRNESSPYAAMQMATNVAQTAMEKGIIGVHVKVRAPGRGKQRSPGPGAQAAIRALARA
                     GMRIGRIEDVTPVPHDSIRGKGGRRGRRV"
     gene            4098..4931
                     /locus_tag="PK154_06125"
     CDS             4098..4931
                     /locus_tag="PK154_06125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HPW10674.1"
                     /translation="MEIIFSQLDENNGRFQLAGCTTPFANSIRRAMIGEVPTLAIETV
                     KIYDNTSALFDEMLTHRLGLIPIRSNEDCLVPQSRCSCGGEGCPACSVIFTMSVEGPA
                     MVYSRDLISQDPRISPAEPDVPIVKLIKDQKVVLEARAVLSVGREHAKWQPTTACGYK
                     VHPVIQVSDTCDGCGQCVDECPRGILEVRNKRVAVIEGQLEKCSLCQLCVRACMNTGI
                     GDEPGIKVTTDESRFLFVVEGDGSLPVREIIGQALEYIRTQSSELSAQIEEITGETRD
                     E"
     gene            4924..5292
                     /locus_tag="PK154_06130"
     CDS             4924..5292
                     /locus_tag="PK154_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HPW10675.1"
                     /translation="MNKMNCRKTNPRLASLISLLKNTSRENEVNIWRDIADRLEAPSK
                     NYAEVNLSKINRYATNGETIIVPGKVLGTGILEMSVSVAALNFSESASAKIRQAQGEC
                     MSIEQLISANPKGSKVRILR"
     gene            5297..5719
                     /locus_tag="PK154_06135"
     CDS             5297..5719
                     /locus_tag="PK154_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPW10676.1"
                     /translation="MVTVIDGDGLLLGRLASMVARRAMSGEEIAIVNAEKAVISGSRA
                     RVLANYGQKRSRGSREGGPFFPRRPDHIVKRTIRGMLPYKRTVGSEAFKRVRVYVGVP
                     AEFTGSEREVLEDAHMNRLNTPKFVTIGAVSTFLGAKY"
     gene            5737..6138
                     /locus_tag="PK154_06140"
     CDS             5737..6138
                     /locus_tag="PK154_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW10677.1"
                     /translation="MAKIVNTSGKRKTAIARATLRPGNGIVRINSVPLDIYPNEMVRL
                     KIAEPLLLIPNSIEGIDVAIDVHGGGIMGQAEAVRTALARGILKWHNDPQIKDVYISY
                     DRTLLVNDSRQKESKKPHGRGARKKFQKSYR"
     gene            6171..6359
                     /locus_tag="PK154_06145"
     CDS             6171..6359
                     /locus_tag="PK154_06145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HPW10678.1"
                     /translation="MIPVRCFTCGKVISTAYDEFKRRREAGEDPKRILDDLHMERYCC
                     RRMLLTHKEIIDDLNPYQ"
     gene            6360..6434
                     /locus_tag="PK154_06150"
     tRNA            6360..6434
                     /locus_tag="PK154_06150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6396..6398,aa:Pro,seq:cgg)
     gene            6469..6639
                     /locus_tag="PK154_06155"
     CDS             6469..6639
                     /locus_tag="PK154_06155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HPW10679.1"
                     /translation="MKESYTRYERARIIGARALQISMGAPLLIRTTKIDPLEIALEEF
                     TQDVIPITVKRK"
     gene            6647..>7424
                     /locus_tag="PK154_06160"
     CDS             6647..>7424
                     /locus_tag="PK154_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HPW10680.1"
                     /translation="MTIICEIRLRKILDSRGNPTIEADVYAETGFGRAAAPSGASTGS
                     HEATVREPGSAIEFAEKSLIPSLIGQDSRDQVAIDGILREIDGTDDFSTLGANVAVAV
                     SLANARAAASSLGLELYEYLGGLFVPSLPLPLGNVIGGGAHAEDATEIQEFLVVPTGA
                     SGPKEAVFANAAVHKALRNILKSRGSSCGKGDEGAWAPKITDDEAFEMISEAAGMVSD
                     ELDVTVRLGLDVAASELWKGDGAFYQYRKKNRTTEEQVSYI"
BASE COUNT         1932 a         1717 c         2128 g         1647 t
ORIGIN      
        1 taccgtcacg gtcgatgcac ccatcggaca ggttgcttgc attatgattg agcgcgatat
       61 ctctggagtt cctgtagttg atcgggccgg gataacaggg attgtcacca agtcagatct
      121 gatgaaatcg acctctgtcg gatcgctcac caaacgggta gaagaggcaa tggaggatgt
      181 gatcaccgtg agccgatacc attccctcga ccatgtcata gacctcatga gagagaggaa
      241 tgataaggtc gttgtggtga ataatgatgg gagtctcgcg ggaattgtga ccgagagtaa
      301 cctggcattt tttctgtatt ccaatgtgag ggcggatctc ccggtaaagg acataacgcg
      361 cctcagaaag gaagagtcgg gaggaaaaaa ggtgttgcgc tctgtttacg agaccgcggt
      421 agtggcggaa gatctgatgt cacatcctgt catgaccatt cctcccgatg cattgatcgg
      481 ggaagcagta tccgtcatgc gggatcaatc agtcaacagt ctgattgttg ttgcggacca
      541 ggagataaag ggtattatca agagagacga cattataagg gaagttgcac aatgaccgaa
      601 gaactctttg tcaaggatgt aatgtcacgg ccggttacca tagcaaaggc tgcggtgatc
      661 acggaagcgc tcgataaaat gctgaatgag ggtatcgatc cactgatcgt catgaataat
      721 aattcagttg ttggaacggt atcccgatgg agcgttgctg aaaaactggg aacaaagcgg
      781 aattcatcca tctctcccaa ttcaatccat gtagcgaata cggtggaaga ggatttcacc
      841 acggtctacc ctgaccagga tgtggagatc ctgatcccgc tcctccagac ctacaagatt
      901 gtcgtggtat atgatgagga tcacaggctt atcggtaagg tgaccgctga ggatctgctc
      961 aaggtgtacc gccccaatgg tacgctcgaa gaagtcatgg aagaagcctg cactattgac
     1021 actgaagaac gggtggtgca tctgcggagg aggatgatcg atgataaaat aatcaggttt
     1081 atcgtcaccg atcaggaccg ggtagtaggg atcgtcacag agacagatgt ggcagttgcg
     1141 atgaggaact tccgtgaggt cgtcgatgat aaacatcagg atcaccgaat caggaacctt
     1201 cttgtcaagg atatcatgag taaaccggtt ctttcagtga gtttcggatc gactgtcgat
     1261 caggtgatca acctgatggt tgccaagaag atcagctcgg ttcccgtgat aaggggcgag
     1321 actcttgcag gtctggtgac ccgggattcg ttgatccggg ccctttaact ttttaaaaat
     1381 tgcattccat cgaatttttt tcataatcat tatcatgctc cggcagaaac gaatgtatca
     1441 gctatggata tgaaacccag gacgccagtt caaccaacaa acgacgtctc ccgtctcctt
     1501 aaagggatga gtgcaaccgg gtttcagggg aggaaactcg gcgagtcgct cgacatatgg
     1561 gcacagatgt gccaggatcc tgactgcacc atcctccttg gtctctctgg agcgatgatt
     1621 ccggcaggga tgcagcaatg ccttatcgag cttgttgaaa accggtatgt cgatgttatc
     1681 gtttctactg gcgctaatat cttccatgat gtctgtgaac actatgggat cagtcattat
     1741 ctcggacatc atcaggctga tgattgccat ctcttctcaa aggggattga caggatttat
     1801 gatgtatttg cctacgaaga acagttcaga agtatcgatc tcaaatttgc cgattttgct
     1861 ggcagtatag cgcctttttc ctgttcttca tctgaattta tccggaagtt tgggaggtgg
     1921 atgatcagcc agcaccccga acagcgatcc ctgctctctg aatgtgtgaa aatgaacgtt
     1981 cctgttttta tccctgcatt atgtgattct tcgattggta tcgggctcat ggtggcaagg
     2041 cggaatggcg tagaggctta tattgatcag atcgctgacg tcgatgagat tgcccgtatc
     2101 gtggagggat cgaaaaagac gggggtaatt tacatcggag gaggggtacc caagaatttc
     2161 atccagcaga cccaggttat cgcagatatg tacgatgcag gccaggaagg ccacgcatat
     2221 gccatccagt acacaaccga tgctcctcac tggggaggac tgtccgggtg cacttttgag
     2281 gaggcaattt cctggggtaa agaggcgccc catgccccga gggtccagtg tttttgtgat
     2341 gcaactattg ccctcccgtt cataacgtcg ggtctcatcg cacgaaagat caggagaacg
     2401 aagaattaac ctctttttga gagcaatgca gcatccctgc tttgaagata aatgcagcat
     2461 cctgaattga ttgaacccaa agtgttatta tccgatcata ccaaataata aagcaccatt
     2521 catggagcgg cacaacatgg tgctgcgagt gtcgaaagat gcgagattct cctgttgagg
     2581 tagccaagcc tggtatggcg caggtttgct aaacctgtgt ccccctggga ctcgagggtt
     2641 caaatccctc cctcagcgct ccaaagaatg ctctagtcaa cgagggattg gatggctcag
     2701 gaagaagata tcaagtattt cgtaaggatc agcaataccg acctggacgg aacaaagcct
     2761 gtccacattg cactgaccgg tatcaggggt gtgggtatgc ataccgccca aatcattgca
     2821 tccctggcga aagtagatga gaaggagacc atggggcgcc tcaaagatga ggaggtcgat
     2881 aggatccgcg agattgcgga cacctatacc cagcgcgtcc cgttctggat gatgaaccgg
     2941 ccaaaggatg tctacactgg tgaatcaaaa cacctgcttg ggactgatgt cagtacggca
     3001 cgggatgagg acatcaacct ccttagaaag atcagatgtt acaggggaat caggcacgaa
     3061 acaggtcaga aggtacgcgg gcagagaacg aagtccaccg gcagaacagg gctcactgtt
     3121 ggtgtgaaaa agaagaagga atgaggtgag aggatgggat atccgggtaa gaatcataaa
     3181 caataccaga cgccgaaacg ccccttcgaa aaatcaagga tcgaagagga gacgaaactg
     3241 gctatcgagt acgggctccg gaacaagcgc gaggtatgga aagcccagag ttacctcagg
     3301 cgttaccgga aagcagccag agaacttctc gcactcatgt ccactgggac cgatcgtgct
     3361 ctgttcgatg caaagaagga tgagttgatc gggcacatgc agcgtgccgg gcttcttggg
     3421 ccggatgctg atattgacaa tgttctctct ctcaaagtcc agcagggtct tgagagaagg
     3481 ctccagacgc tcgtgtaccg tcagggcctt gcgcgatcac ccaaacaggc acgccagctg
     3541 atcacccatg gacatattgc aatcgatggg aaacgatgca gtattcccgg ataccgtgtg
     3601 aaaagagctg aggagagcaa gatctcgtat tacgggaaat ccccgctcgc tgcctcggaa
     3661 catgccgaga aagcccggat aacaaaacag gggagataat cgatggctgg tgaaaaggaa
     3721 aagtggggaa ttgcccatat ttatgcttca ttcaacaaca ccatcatcac ggtaaccgat
     3781 ctttcgggag cggaaactgt cacgaagagc agtgggggta tggtggtgaa gcaggaccgg
     3841 aatgagagtt ccccctatgc agccatgcag atggcgacca atgttgccca gactgcaatg
     3901 gaaaaaggaa tcattggggt gcatgtcaag gtacgtgcac cgggtcgcgg aaaacagcga
     3961 agccccgggc ctggagccca ggcagccatc cgtgccctgg cacgggcggg gatgagaatc
     4021 ggcaggatag aagatgtcac tcccgtccct catgattcaa tcaggggaaa gggtggcagg
     4081 cggggaagga gagtctgatg gagatcatat tttcccagct tgatgagaat aacggccggt
     4141 tccagcttgc aggatgcact accccttttg ccaactccat acggagagca atgatagggg
     4201 aggtccctac actcgcaatt gagaccgtga aaatttatga caataccagt gctctcttcg
     4261 acgagatgct cactcatcgg ctcggcctta ttcccatcag gagcaatgaa gactgccttg
     4321 taccgcagtc ccgctgttcc tgtggcggag agggatgtcc cgcatgcagt gtcattttca
     4381 ctatgagcgt tgagggacct gcgatggttt attcacggga tctcatctcg caggatccaa
     4441 ggatcagtcc cgcagaaccc gatgttccaa tcgtgaagct catcaaggat cagaaagtcg
     4501 tccttgaggc acgtgcggta ctgtcggtag ggagggaaca tgcaaaatgg cagcctacca
     4561 cagcctgcgg atacaaggtc cacccggtga ttcaggtcag tgatacgtgc gatggatgtg
     4621 ggcaatgcgt cgatgagtgt ccccggggca tcctcgaggt caggaacaag agggttgccg
     4681 tcattgaggg gcagcttgag aaatgttcgc tctgtcagtt gtgtgtgcgg gcatgcatga
     4741 ataccgggat cggtgatgaa cccgggataa aggtgacaac cgatgagtcg cggttcctct
     4801 ttgtggttga gggtgacggc tctctccctg tcagggagat catcggtcag gcactggaat
     4861 atatcaggac acagtcctct gaactttcag cgcagattga agagataaca ggagagaccc
     4921 gggatgaata agatgaattg cagaaagaca aatccccgac ttgcctccct gatctccctt
     4981 ctgaagaaca cttcgaggga gaatgaggtg aacatctgga gggatatcgc agaccggctc
     5041 gaagctccca gtaagaatta cgccgaggtt aacctgagca agatcaaccg gtatgcgaca
     5101 aacggagaga cgattatcgt ccccggaaag gtcctcggca ccgggatcct cgagatgtct
     5161 gtgagcgtag ccgcccttaa cttctctgaa tctgcatcag cgaagatcag gcaggcgcag
     5221 ggtgagtgta tgagcatcga gcaattgatt tcagccaacc ccaaggggag caaagtacgg
     5281 atactgaggt gatgagatgg tgacagttat cgacggtgac gggttgctcc tcgggcgact
     5341 ggccagcatg gttgcccggc gcgcgatgag tggcgaggag atcgccattg tcaatgcaga
     5401 aaaagcggtc atctcgggga gcagggctcg ggttcttgcc aactatggtc agaaaaggag
     5461 ccggggatcc cgtgagggag gcccgttttt ccccagaagg cctgatcata ttgtgaaacg
     5521 gactatcaga gggatgctcc cttacaagag gacggttgga agcgaggcct tcaagcgtgt
     5581 ccgggtctat gtaggtgtcc ccgcagagtt tactggcagt gagcgtgaag tccttgaaga
     5641 tgcgcacatg aaccggttga acacccctaa gtttgtcaca ataggggcgg tcagcacgtt
     5701 ccttggagcg aagtactaat gaggggtgaa gagtatatgg cgaagattgt taacacaagt
     5761 ggaaaaagga agactgcaat cgcccgggcg acgctcagac cggggaatgg catcgtcagg
     5821 atcaactcgg tcccccttga tatttatccg aacgagatgg taaggttaaa gatagcggaa
     5881 ccactcctcc tgataccgaa ttccattgaa ggaatcgatg ttgcaattga tgttcatggt
     5941 ggtgggatca tggggcaggc agaggcggta cggaccgcac tcgcacgcgg tatcctcaag
     6001 tggcataacg atccccagat caaggatgtc tacatctcgt atgaccgtac cctgctcgtg
     6061 aatgattcac ggcagaaaga atcaaagaaa ccccatggcc ggggggcaag gaagaaattc
     6121 caaaagtctt atcgttgatc tgccaaaaca aagtaggaga tcggaccgtt atgataccag
     6181 ttcggtgttt cacgtgcggg aaagttattt ccacggcgta tgatgagttc aaacggagac
     6241 gggaagcggg agaggatcct aaaaggatcc tcgatgatct ccatatggag cgatactgct
     6301 gtcgtcgcat gttgctgacc cataaagaga taatcgatga tctcaatccg tatcagtgag
     6361 gggtcgtggg gtagcctggc ctatcctatg gcgttcggga cgctgtgacc tgagttcgaa
     6421 tctcagcgac cccattcaaa aaccattcat ggaacatttt gaggttatat gaaagagtcg
     6481 tacacccgat atgagagagc ccggatcatt ggagcccgtg ctctacaaat atcgatgggc
     6541 gctccattgc ttatcaggac cacgaagatc gatccgctgg agatcgctct cgaggaattt
     6601 acacaggacg ttattcccat cacagtaaag aggaagtagg aattacatga ccattatctg
     6661 cgagatccgc ttaagaaaga tactggacag cagaggtaat cccaccatcg aagctgatgt
     6721 ctatgccgag accgggtttg ggagggctgc tgctccgagc ggcgccagta ccggttcgca
     6781 cgaggcaacg gtaagggagc ctggatctgc catcgagttc gctgagaaga gtctcatccc
     6841 gtcgctcatc gggcaggatt cccgtgacca ggtcgccatt gacggcatcc tccgcgagat
     6901 tgacggcact gacgactttt caactcttgg tgcgaatgtt gctgtagcag tctctctcgc
     6961 caacgccagg gcagccgcct cttctcttgg ccttgagctg tacgagtatc tgggaggcct
     7021 ctttgtccct tccctgcccc ttccgctggg caacgtgatc gggggaggtg cacatgcaga
     7081 ggatgcaaca gagatacagg agtttctcgt agttccaaca ggggcatccg gacctaaaga
     7141 agcggtgttt gcgaatgctg cggttcataa ggctctcagg aatatcctga aaagccgtgg
     7201 gagttcctgc gggaagggcg atgagggagc ctgggcccca aagataaccg atgatgaggc
     7261 atttgaaatg atcagtgagg cggctggtat ggtttccgat gaacttgatg tcaccgtcag
     7321 gctgggtctc gatgtcgctg caagcgagct ctggaaggga gatggtgctt tttaccagta
     7381 ccggaaaaag aaccggacaa cagaggagca ggtctcttat atca
//
LOCUS       DAOOCO010000089         7361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9493_length_7361_cov_5.490966, whole genome shotgun sequence.
ACCESSION   DAOOCO010000089 DAOOCO010000000
VERSION     DAOOCO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9493_length_7361_cov_5.490966"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2295)
                     /locus_tag="PK154_06165"
     CDS             complement(106..2295)
                     /locus_tag="PK154_06165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF002654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPW10681.1"
                     /translation="MNVEDLPLSKDLIGLYWSSGIHELYPPQAQCVDRGIFKGKNLLV
                     AIPTASGKTFIAEIAMHYHFKKGGKCLYIVPLKALASEKFEEFSGKGAKVGISTGDYD
                     RRDDYLGRNDFIIATSEKVDSLLRNASSWLKAITLLVIDEVHLIDSPDRGPTLEMVIA
                     KMRRMNPGMQVIALSATIGNPGMLARWLDADLVTSSWRPVDLRQGVYYDGAIHFQTET
                     RSVPSISRHESVNLVMDTIAEGGQCLVFVSSRKNAEAFAKQAAKAIRIDSEDLRKYRQ
                     SLEKLASTEQEKALALCVGSGAAFHHAGLRKEARTLVEEGFRKGHIRCIASTPTLAAG
                     LNLPARRVIIKEYRRYSSGEGMVPIPAREYHQMAGRAGRPHLDPYGEAILITTDARAA
                     GQLFDYYIEAPPEDVHSQCSSEEALTTHILSLIASGFIRTRPELREFMRSTFYFHQKK
                     QDRILESVISRALTFLEQAEMIVQQDDLLSATEYGRIVSLLYIDPTSADTILEGMLAA
                     EEYSDIGLLHLICSTPDMYTLFVGNRDSHYLDRFVLEHEHELWIDCPFDDSENYFRAV
                     KTAMVLYDWSREVPEAIIGERYSIGSGDIYNLLEGANWLLHASSRLSRMFRGVFASSV
                     QEFEYCMKHGIKRELIPLVKIRNIGRVRARRLFNNGFTTPEKILEAGEERIAPILGQG
                     VTSMIFRDLRGTASTIQKMATDSIMQKEAAPRFQPTLFQFGDENNED"
     gene            complement(2452..2991)
                     /locus_tag="PK154_06170"
     CDS             complement(2452..2991)
                     /locus_tag="PK154_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPW10682.1"
                     /translation="MIQEIKIAGNRVGVLIGKNGSVKKELEEKTSTSISIDSKEGSVK
                     LEGEDAPLLLRGIEVVQAVNRGFSPERAFMLLEDEDLLLDVIDLSGLTDSPRQLDRLR
                     GRIIGKDGRSREQIEHMTECEISVFGKTVAIIGLPEQIKIARSAVDMLLQGLPHEMVF
                     SFLERKRREMKQDMIDYYY"
     gene            complement(2996..3790)
                     /locus_tag="PK154_06175"
     CDS             complement(2996..3790)
                     /locus_tag="PK154_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HPW10683.1"
                     /translation="MSLDRIESNFDREVERLGIRIKDMDQLKVRDDVFDEVTLMALYK
                     LVHKGWITAIGGSISTGKEANVFLGERGDQDIAIKIYRIRTANFNAMSEYIIGDRRFA
                     GIKRTKKDLIFTWTRKEFANLKRAADAGLSVPMPYVWDRNILIMGFLGEDERPFPHLR
                     AVELENPAGVYYEVLSFMRGLYQQAGLVHADLSEFNILYGDRLYVIDMGQSVTPDHPR
                     AKFFLARDIANINRYFRDFCTVMDEKEIFLKITDGTSEGFPPRGWE"
     gene            complement(3806..4744)
                     /locus_tag="PK154_06180"
     CDS             complement(3806..4744)
                     /locus_tag="PK154_06180"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW10684.1"
                     /translation="MDPYSLVTRNAVEVVTDDELRALLVKSKKRVYAGYEPSGEIHLG
                     HLVTVNKLIDLKEAGFEVVVLLADLHAFLNRKGTMERVGELAEYNKRCFEGLGLRDVE
                     YVLGSDLQLSPDYQLMVLRLSQQITLNRAKRSMDEVGRNMDAPTVSQMIYPIMQMVDI
                     ASLNVDAAVGGIDQRKIHMLAREHLVSIGYPAPVCIHTPILNGLDGKKMSSSSGNYIS
                     VADSVDEIEKKCQKAFCPPECEENPILQMFQYHIFPRSGPVTVKRPDKFGGDREFLRF
                     EDLEEAYRKGEIHPLDLKKTAGRYLTEILSCVRDYV"
     gene            complement(4805..5038)
                     /locus_tag="PK154_06185"
     CDS             complement(4805..5038)
                     /locus_tag="PK154_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10685.1"
                     /translation="MIPLRAASIKDENAGGIDPWCQGADALVHPERSNIKGEDVQPIP
                     LPKHSGRIFYPNSIYLYLEDKNPVERENRHKLV"
     gene            complement(5525..6547)
                     /locus_tag="PK154_06190"
     CDS             complement(5525..6547)
                     /locus_tag="PK154_06190"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HPW10686.1"
                     /translation="MINVAINGYGTIGKRVADAVAAQPDMKVIGVSKTKPSHEAFVAK
                     SRGYPLYIADIAKKAAFEKAGLPVAGAVEDMLKAADIVVDATPGGIGEKNKPVYERLG
                     VKAIWQGGEDHDVAGFSFNSDCNYKEAIGKQFTRVVSCNTTGLCRIINAMDKAYGVEK
                     VRAVMVRRGGDPADIKRGPIDAIVLNPVHIPSHHGPDVQSVLPNINIVTLAMIVPVTM
                     MHMHVVQMDLKREATREKVLEIIEKHPRMGLVRKATGITSTAELKEYAMDLGRHRSDL
                     WENGIFEESVSCLGNELYLFQAIHQEADVVVENIDCIRAMTGIEKDPAKSVAMTNKAL
                     NFIAIQ"
     gene            complement(6793..7275)
                     /locus_tag="PK154_06195"
     CDS             complement(6793..7275)
                     /locus_tag="PK154_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW10687.1"
                     /translation="MNSGTTGAEILLVEDNPNDVELILHVFQWCNLGDRIHVAWNGEE
                     ALDYLFGTGTYEGKSIGRPKVILLDLKLPKVDGLEVLRKIKNEPATHSIPVVIFTSSR
                     EERDIVESYALGVNSYIVKPVQFDQFANVIRDLGLYWQQINQPPVENSKGNNIRNPSG
                     "
BASE COUNT         1684 a         1984 c         1813 g         1880 t
ORIGIN      
        1 gcgattggtc caggtactac ccactgctca tcatgcaata ggaggctctt cttgccgaat
       61 actcaagcca cccaggcagg aagaatctcc tttgcatcat acaaatcaat cttcattatt
      121 ctcatctccg aattggaaga gggtaggctg gaacctcgga gctgcttcct tctgcatgat
      181 actgtcagtt gccatctttt gaatggtgct ggcagtcccc ctcaggtctc tgaatatcat
      241 gctggttacc ccctgcccga ggattggcgc gattctctct tctcccgctt cgaggatctt
      301 ctcaggagtg gtaaagccat tgttgaacaa ccgtcgtgct ctgacacgtc cgatattcct
      361 gatcttcacc agggggataa gttcgcgctt tatgccatgt ttcatacaat attcaaattc
      421 ctggaccgat gaggcaaaga cacctctgaa catcctgctg agccggctgg acgcatgcag
      481 gagccagttg gccccttcaa ggaggttgta aatatcaccc gatcctatgg aatacctctc
      541 accgatgatc gcctcgggaa cctcgcggct ccagtcatag agtaccatag cggtcttcac
      601 cgctctgaaa taattctcag agtcatcaaa tgggcagtca atccagagct catgctcatg
      661 ctcgagcaca aaccggtcca ggtaatggct gtctctgttc ccgacaaaga gagtgtacat
      721 atctggggta ctacagatca aatgcaggag accgatatcc gagtactcct ccgcggccag
      781 cattccttcg aggatagtat cagcactagt tggatcaata tagagtaatg agacgattct
      841 cccatattct gtcgccgaga ggaggtcatc ctgctgcaca atcatctctg cctgctccag
      901 gaatgtgagt gcccggctga tgactgattc caggattctg tcctgtttct tctggtgaaa
      961 gtagaaggta gagcgcatga actcacggag ttcgggacga gtgcgtataa agcctgacgc
     1021 gatcagcgag agaatatgtg tcgtgagagc ctcttctgaa gagcactgtg agtgcacatc
     1081 ttccggggga gcctcaatat aataatcgaa gagttgccct gcagctcgtg catcggtagt
     1141 gataaggatg gcctcaccgt acggatcgag atgaggtcgc ccagccctgc cagccatctg
     1201 atggtattca cgtgcgggaa tggggaccat accctccccg ctgctgtatc ggcggtactc
     1261 tttgataatg accctgcggg ctgggaggtt gagccctgca gccagcgtgg gagtcgaagc
     1321 gatgcacctg atatgtcctt ttcgaaagcc ctcctctacc aatgtccgtg cctctttccg
     1381 aagccctgca tgatggaacg cagccccgct cccaacgcag agtgcgagcg ctttctcctg
     1441 ttccgtacta gccagctttt cgaggctctg cctgtatttc cggaggtcct cactatcgat
     1501 acggattgct tttgccgctt gttttgcgaa tgcctctgca ttctttctcg atgagacaaa
     1561 taccagacac tgacccccct ctgcaatcgt atccataaca agattgacac tttcgtggcg
     1621 ggagatggag ggcacagacc gcgtttcggt ctggaagtgg atggcaccgt catagtatac
     1681 cccctgcctg aggtccacgg gtctccatga gctggttacc aggtcggcgt caagccagcg
     1741 tgcgagcata cctggatttc ctatcgttgc cgatagggcg atcacctgca ttcctggatt
     1801 catccgcctc atcttggcga tcaccatctc gagtgtcgga ccccgatccg gggaatcgat
     1861 caagtggacc tcgtcaataa cgagcagtgt gatagccttc agccatgatg atgcattacg
     1921 gagcagcgaa tcgactttct cgctcgttgc aatgataaaa tcgttcctcc caaggtaatc
     1981 atcacgccgg tcatagtcgc cggtggagat gccgaccttt gcacccttcc cgctgaactc
     2041 ttcgaatttc tccgaggcaa gtgccttcag gggcacgatg tacagacact tgcccccttt
     2101 tttgaaatga taatgcatcg caatctctgc gatgaaggtt tttccactgg cagtcggtat
     2161 ggctacaagg aggttttttc ccttaaaaat ccccctatcc acgcactgtg cctggggagg
     2221 atacagctca tggattcctg atgaccaata cagcccgatc aggtccttgg agagtgggag
     2281 atcttcaacg ttcatccata ccccttttat tccagtctat atcactagca ggttagttct
     2341 ttttcctaac catctctttg ccgcgcatga aaatctccaa tggaaatata cctctctccg
     2401 cgtaaaaacc acctggttcc ggtctgatcc gggtcagaaa caccattgat atcagtaata
     2461 ataatcgatc atatcctgtt tcatctcgcg gcgcttccgc tccaggaatg agaacaccat
     2521 ctcgtgcggg agtccctgga ggagcatatc aacagcactc cgcgcgatct tgatctgctc
     2581 tgggaggccg atgatggcaa ccgtcttgcc gaataccgaa atctcgcact cggtcatatg
     2641 ctcgatctgc tcccgcgaac ggccatcctt gccgataatt ctccccctga gccggtccag
     2701 ctggcggggg ctgtcggtaa gacctgacag atcgatcaca tccaggagca gatcctcatc
     2761 ctcaagcagc atgaatgccc gttcaggtga gaaaccgcgg ttgactgcct ggacgacctc
     2821 gatacccctg agaaggaggg gtgcatcctc gccctccagt ttcacactcc cttccttgct
     2881 gtcgatactg atgctggtac tggtcttctc ttcgagttcc ttttttaccg agccattctt
     2941 tccgatcagc accccgacgc ggtttccagc gatcttgatc tcctgtatca ttcttttact
     3001 cccaaccccg agggggaaat ccctcgctcg ttccatcggt aatctttaaa aaaatttcct
     3061 tttcatccat gaccgtgcag aagtccctga agtaccggtt tatattggca atgtcccggg
     3121 caaggaaaaa tttcgcccgg gggtgatcag gggttactga ctgtcccata tcgattacgt
     3181 acaagcggtc gccgtaaagg atattaaact cagacaggtc ggcatggaca agcccggcct
     3241 gctggtagag tccccgcatg aatgagagca cctcataata aactccggcc gggttttcca
     3301 gttcgactgc cctgaggtgt ggaaaaggac gctcatcctc gccaaggaat cccatgatca
     3361 ggatattccg gtcccataca tacggcatag gtactgaaag tcccgcatcg gcagccctct
     3421 tcaggtttgc aaactctttt ctggtccagg taaaaatgag atcctttttt gtccgtttta
     3481 tgccggcaaa ccgcctgtca ccgatgatgt actcactcat tgcgttgaag ttcgccgtcc
     3541 ggatacgata gattttgatg gcgatatcct ggtcaccccg ctcaccgaga aagacattcg
     3601 cctccttgcc ggtactgatc gagcctccga tcgcggtaat ccagccttta tggaccagtt
     3661 tgtacagtgc catgagggtc acctcgtcaa agacatcgtc tcggactttc agctggtcca
     3721 tgtcctttat ccgaatcccg agccgttcga cttcgcgatc gaagttgctc tcaatccgat
     3781 caaggctcac ggtgcgaatc cctctctata catagtccct gacacaggag agtatctctg
     3841 ttagatagcg tccagctgtc ttcttcaggt caagggggtg gatctcccct ttccggtacg
     3901 cctcctccag atcttcgaac cggagaaatt cccggtcacc cccaaacttg tcaggccgct
     3961 ttacagtgac cgggcccgac cgcgggaaga tatgatactg gaacatctgc aggatcggat
     4021 tctcttcgca ttcggggggg cagaaggctt tctggcactt cttctcaatc tcatcgactg
     4081 aatcggctac cgagatgtaa ttgccggaag aagaggacat cttctttccg tcgagcccgt
     4141 tgaggatggg ggtatggata cacaccgggg ccgggtatcc gatactgacg aggtgctccc
     4201 gtgccagcat gtggatcttc cgctggtcaa tccctcccac agcggcatcg acgttcagcg
     4261 aagcgatgtc caccatctgc atgatcgggt agatcatctg ggacaccgtc ggggcatcca
     4321 tgttcctccc gacctcgtcc atgctccgtt ttgcacggtt cagggtgatc tgctgggaga
     4381 gcctgagcac catcaattgg taatcgggag agagttgcag gtcggatccg agcacgtatt
     4441 cgacatcacg gagaccaagt ccttcgaagc accgcttgtt gtactcagca agctcgccaa
     4501 cccgctccat ggttcccttc ctattgagga acgcatggag atccgcgagg aggacgacta
     4561 cttcgaaacc ggcttccttc agatcaatca gcttgttgac tgtaaccaga tgcccgaggt
     4621 ggatctcacc gcttggttca tacccggcat agaccctttt tttgctcttt accagaagag
     4681 ctcttagctc gtcatctgtg acgacttcaa cggcatttct cgtcaccagc gaataaggat
     4741 ccatttacaa tgaatgggtt gtggtgggtg ttaatgatta tgagaggaaa aaaatgtcca
     4801 gcgtttatac caatttatga cgattttctc tctcaaccgg attcttatct tccaggtaca
     4861 gatagataga atttggataa aaaattctcc cggaatgttt agggagaggt attggctgaa
     4921 catcttcccc cttgatattg gatctctccg gatgaacaag agcatcagct ccttggcacc
     4981 aggggtcaat gcctccggca ttttcatctt ttatgcttgc agccctcaaa ggaatcatgt
     5041 tattattcta gatatccggg aatgatgaaa aacgaatgat aaggtggcag ttcaacaccc
     5101 gttttttcac atctcttaat ttcacatctc ttaagaaggt acattgcgta agagacactc
     5161 ctgatggtta tgcctgcgat gcgataggac atgctcaaga ccacaaattt ctacataatt
     5221 ctcggatgaa tggggaatca ctttttacat tgatgtgaac actccgacta cggatttttt
     5281 ccaatgtgat ttatgagaac ggttggtggt gaaaataaga ctaatcctaa aagaagagtc
     5341 agaactgcac gatcactgct tgtaaatcgg gtttctgcaa ggggggggcc ctcctgataa
     5401 cggatctctc aagatgacgg gaaggtcagg gtatatcaga aatgaaaaag gctgaatcct
     5461 cattatcggc acctcttccc ggatttaaaa aaagaacagg gagacccgtg ctactgctct
     5521 ccggttattg tatcgcaatg aaattgagtg ccttgttggt catcgccacc gattttgccg
     5581 gatctttctc gatcccggtc atcgcacgga tgcagtcgat attctcgaca acgacatcgg
     5641 cttcctggtg gatagcctgg aagaggtaca actcgtttcc gaggcaggat accgattcct
     5701 cgaagatccc attctcccag aggtccgagc ggtgacggcc caggtccatg gcatactctt
     5761 tcagttcggc ggtactcgta atcccggtgg ccttccggac gaggcccatc cgtggatgct
     5821 tctcgattat ctcaagaacc ttctcacggg tggcctctct cttgaggtcc atctggacaa
     5881 catgcatgtg catcatggtc acgggaacga tcatggccag ggtcacgata ttgatgttcg
     5941 ggagaacgct ctggacatca ggaccgtggt gacttgggat atgcacggga ttcaggacga
     6001 ttgcatcaat agggccacgc ttgatgtcgg ccgggtcacc tccccttctc accatcacag
     6061 cccggacctt ctccacccca taggccttgt ccatggcatt gatgattctg caaagcccgg
     6121 tcgtgttgca ggagactacc cgggtgaact gctttccaat ggcttccttg tagttgcagt
     6181 cggagttgaa cgagaatcct gccacatcat ggtcctctcc tccctgccag atggctttaa
     6241 ctcccagtct ctcgtatacc ggcttgtttt tctccccgat tcccccaggg gtggcatcca
     6301 ccacaatgtc ggcagctttt agcatatcct cgactgcccc ggcgactggc aggcctgcct
     6361 tctcaaaggc ggccttcttg gcgatatcgg caatgtagag gggatagcct ctcgatttgg
     6421 cgacgaatgc ctcgtggctg ggttttgtct tcgacacccc gatgactttc atatccggct
     6481 gtgcagcaac cgcatcggca acgcgctttc cgatcgttcc atatccgttg atggctacat
     6541 tgatcatatg ggagggatgt cggaggagta gcaattaaat gttggggttt tgggccattg
     6601 ggtgggatgg aaaaccccct ctgggaaaag atatgatcag ggtgaatatg cgcaacatca
     6661 tcctatgaaa gaaaaaggaa acggaaaaat cccagagaat tcatcgagaa agtcgtttgg
     6721 aatctttgga ggcatatcga ttcaaaaagg aaaggaaggg aaaatacccc gtccggcaca
     6781 attctggcct gttcatccgc tgggattcct gatgttattt cccttgctgt tttcaaccgg
     6841 cggctgatta atttgctgcc agtagaggcc gagatcccgg ataacgttgg cgaactgatc
     6901 aaactggacc ggcttgacga tatagctgtt tactccgagt gcatagctct cgacaatatc
     6961 acgctcttca cgggaggagg taaaaatgac aacgggtatt gaatgagttg cgggttcatt
     7021 cttaattttc cggagtactt ccagaccatc cactttgggc agtttcagat caagcagtat
     7081 gactttcggc cggccgatgg atttgccttc gtaagttcca gtgccgaaga ggtaatccag
     7141 tgcctcttca ccattccagg caacatggat cctgtctcca agattgcacc actgaaaaac
     7201 gtgaaggatc agctcgacat cgttgggatt gtcttccacg aggagtatct ctgcaccggt
     7261 cgtgcccgag ttcatgtttg atactagccc agcgtaaaga aaaaggttgt gccctctccc
     7321 tggactgact cagcccagac acgacccccg tgacggtgaa t
//
LOCUS       DAOOCO010000090         7323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9555_length_7323_cov_5.400798, whole genome shotgun sequence.
ACCESSION   DAOOCO010000090 DAOOCO010000000
VERSION     DAOOCO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_7323_cov_5.400798"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..969)
                     /locus_tag="PK154_06200"
     CDS             complement(52..969)
                     /locus_tag="PK154_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPW10688.1"
                     /translation="MSQDRLLQMVVSVLLMAGFRVTERFSMRPRSFDLITGKKEILLV
                     IKVVSHIDSVSEETARDLDHIARSLRGSPLIIGERARDAELERGAVYVRYGIYAVSVA
                     TLYDAFVEDNPPLVYASPGGLYVNINGEILREQREKRNLSLGDLGNLLGVSRRAISKY
                     EGGMGTTLDIALRIEEIFDAALVRSIDLLKYESHFRDEPEQGIDEMPMSFLERLGITL
                     HAMHRAPFQAMIEFSDHTILTGYGTTDKVVKRAALIGNISQVAGTRAMCVLTDYYKQK
                     KIGSTLVIGEERLHSLADGEELIELIDKS"
     gene            1020..2264
                     /locus_tag="PK154_06205"
     CDS             1020..2264
                     /locus_tag="PK154_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HPW10689.1"
                     /translation="MRIGIDDTDSPAGMCTTYLGALLAGRLSDSGMTVRETYLIRLNP
                     NVIHKTRGNAAICIDVIGDISMAFSMACDIVEELADFSCEETNPGVVVSDRALPAEFY
                     KRAVTDFCEISEAVTLLEESEALFRGYKNGRGLIGAAAAVSSVLEDSTAEILVYRRPD
                     CRGYPRMVDRKSLFLADGETYPHTWDTVDRENNIVVCVPHTPDPVLFGIRGTSGEWVL
                     RARRMVISEAPEREQIFTTNQGTDAHLIAGSPGALEPGRSYLVRGTVSTTPMTRSGGH
                     VSFYLSEGGFSVRCMAYEPTKGFRDIIRMLTAGDEVVVAGSYKGGSINLEKIGVMSIV
                     PRIEQKAPFCSSCRKRMTSAGKGKGYKCRICGAREQDPERVYLARELKPGWYEVAPSA
                     RRHLAKPLCRGHPDLAKYGIGD"
     gene            complement(2261..5326)
                     /locus_tag="PK154_06210"
     CDS             complement(2261..5326)
                     /locus_tag="PK154_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPW10690.1"
                     /translation="MPLPPAITRLIEEYRDSSPAYREGDISRQTLCRDFIDPFLESLG
                     WERQAGDSLGQSSGLLLDVPVRIGGQAGSIDYILCSQKGGALAIMVADCSPAEESAFL
                     LLRYAWNAGLDLAILTNFRESALYNTTTPVQCRDDVAGALITRLGIGQYEEHWDHIAA
                     LLSREGITRDSIGRFLKGQGIRETSRIGEVLRKDLEAWRAHLAKKIALHNHGVTEDSL
                     SDLVHALLIRILFLRIIEDKGLGQEGVLMRITGGGSAYGRLCGLFRYACEEYGGGLFC
                     CSERGGGLESPESIIYSLSVDDDTIKNIIARLSPEKGPYEFSVIPARVLASVFSPYLG
                     SMIRIRNGHQAVIEECSEFRKYGNPSPVPFEMAEYVVRRTLSNLVKEKTVQELQHLHL
                     LDPACGSGLFLVMAFEFLLEWHRAWYFRNLLPVLKQGNFSHDDIQPFLPDGAERKGAD
                     SILPFIIIHGTEGIHPVKQLTIRLTPAERRRILLSILSGVDIDRRAVEITKVILLVAL
                     MEGGDRQGLPLPDLSSCIRCGNSLVGPDFLDDPETALIGPDTRERISVFDWDSVFPRI
                     IGSGGFDGIIGSFPAHRCESLKGEKGYLEKHYRCYPQSGELYPCFVEKGIFLLKQGGL
                     LSAAFPDVWLRSKHGSKLRHFLSGSGIQEIIAPGIRERENLFIPRTCIVTIAKEEPGA
                     SISVVQGVIPATGNFVEYLNKNRYCIPRWRIGQGSWILADTCTQDLVSKIQGEGTPLA
                     EYVMGALSRGIKRADDSPLLIDSVSKKRVIAEDMESATIIRPFLTPERLKRYYFLPPK
                     WHIILTPSGAIGKEPPVLVHQPRNQSKEMVRKERRMDEGTDKKTMPDVNTLFPLPDKG
                     QHSDLIASPKILFPCIARESRFSLDNTGHCCPDTCCMVISSSLYLLGLLNSRLIWFLI
                     NETLPPLPGGYRKLWGPSFENIPIYVPDFENREDVAIHDTIETLVRQMIALHRKISEI
                     EDPTIKATYWKQIGEIDHAIDHLVYDLYQLTPGEIGIIEKAVSGPDYSA"
     gene            5459..6388
                     /locus_tag="PK154_06215"
     CDS             5459..6388
                     /locus_tag="PK154_06215"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPW10691.1"
                     /translation="MECGDPVEQVTIRPGMSVNDLVCELARAGAYNGGALARAADIYE
                     RMLTDKKTVKFFGLAGAMVPAGMGGIVSSLLTRGYIDLLVSTGANLTHDIIEALGCRH
                     YHGSAFCSDIELRQEEINRIYDVFLPNEAFATFETFIQESLSTLDPGHSYPISELLAL
                     LGKRLDHGILATAAARGIPVYCPAVADSMIGLQCWLYSQSGKISVDAFSDMKGLIDRC
                     FTAEKAGALLVGGGVPKNFILQSMLMTPQGFTYAVQLTGDRPDLGGLSGATLDEARSW
                     GKITGDALAVTVYGDATITLPVLVAAVLERMER"
     gene            6385..7020
                     /gene="pyrF"
                     /locus_tag="PK154_06220"
     CDS             6385..7020
                     /gene="pyrF"
                     /locus_tag="PK154_06220"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPW10692.1"
                     /translation="MTQLILALDVSGRTEALRIGRQCAPHIDAIKVGYPLVLSAGLTI
                     ARDLAATGLPLIADFKVADIPNTNRLIAEQVFSSGFSAIICHGFPGSDSAEACIESAH
                     DHDGECYLVAEMSHPGAEEFFHGGVAEHIAELAVTLGADGIIAPATRPDRVRLLRGIV
                     RRKKIYSPGVGAQGGDAAIVAGLVDGIIVGRQIYLSPDPRSAAAEYGPFRQ"
     gene            7119..>7323
                     /locus_tag="PK154_06225"
     CDS             7119..>7323
                     /locus_tag="PK154_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPW10693.1"
                     /translation="MQWSEEQQKLAEKYKKLGDIPVAERRYKCHTCHLIVDEKPCPQC
                     GDEHLVIMCPLDHCHCSHEIISGI"
BASE COUNT         1715 a         1981 c         1947 g         1680 t
ORIGIN      
        1 agatattcac cacttgtagc gataaaattg gtttgtggtt ctatataaaa tttatgactt
       61 atcgatcagc tcgatcagct cttcaccgtc tgccaatgag tggaggcgct cctccccaat
      121 caccagtgtg ctgccgatct ttttctgctt gtagtaatcc gtcagcacgc acatggcacg
      181 agtacctgcg acctgggaga tattcccgat gagggctgca cgcttgacca ccttgtcggt
      241 ggtgccgtag ccggtaagaa tcgtatgatc ggaaaattcg atcatcgcct ggaagggtgc
      301 ccggtgcatt gcatggagcg tgatgcctaa cctctcaagg aaactcatcg gcatctcatc
      361 gatcccctgc tcaggttcat ccctgaagtg cgactcgtac ttcagcagat cgattgatct
      421 gaccagcgcg gcatcaaaga tctcttcaat ccggagtgcg atatcgagcg tggtccccat
      481 acctccctca tacttgctga tcgccctccg ggaaacacca agcagattgc caagatcgcc
      541 gagggacaga ttccttttct cccgctgctc acgaagtatc tcaccattga tgtttacgta
      601 gaggcctcca ggagaggcat acaccagggg aggattgtct tcaacaaaag catcgtaaag
      661 tgtggcaaca ctgactgcat agatgccgta ccggacatac acagctcccc gctcaagctc
      721 cgcatcacgg gcccgctctc cgataataag gggagatccc cgtagggaac gtgcgatgtg
      781 atcgagatcc cgtgccgtct cctcactcac actatcgata tgggagacca ccttgatcac
      841 aagcaggatc tccttctttc ccgtaataag gtcgaaactc cgtggccgca tggagaaccg
      901 ttccgttacc cggaacccgg ccatgagcag taccgacact accatctgga gaaggcgatc
      961 ctgggacatt tccgttacga atggatatag cagggcagga tattaatagt agtgcataca
     1021 tgcgaatcgg aatcgacgac acagattcgc cagccgggat gtgcaccacc taccttgggg
     1081 ctctgcttgc cggcaggctc tccgattcgg gaatgaccgt gagagaaacc tacctcatca
     1141 gactgaatcc caatgtgatc cacaagacaa gagggaatgc agccatctgc atcgatgtca
     1201 tcggtgatat cagcatggct ttttccatgg cctgcgatat tgttgaagaa ctggcagatt
     1261 tctcatgcga agagaccaat ccgggtgttg tcgtttcaga tagggcgctt cctgctgaat
     1321 tctacaaaag agcggtcact gatttctgcg agatcagcga agcagtcaca ttgcttgagg
     1381 aatcggaagc gctcttcagg ggatacaaga acgggcgtgg tcttattggc gcagccgcgg
     1441 cagtctcaag cgtcctggaa gacagcacag ctgagattct ggtttaccgc aggcccgact
     1501 gccggggata tccacggatg gtggaccgca agagcctctt tcttgctgat ggtgagacct
     1561 atcctcacac ctgggatact gtagacagag agaataacat tgttgtctgt gtcccccata
     1621 cccctgatcc ggtactcttt ggaataagag gtacttcggg cgaatgggtg ctccgtgcac
     1681 gaaggatggt gatatctgaa gctcctgaga gggagcagat ctttaccacc aaccagggca
     1741 ccgatgctca cctgatcgcg ggatctcctg gtgcactgga acccggaaga tcctatctgg
     1801 tcaggggaac ggtctccacg acaccaatga cccggagtgg agggcatgta tcgttctatc
     1861 tttcagaagg cggcttttcc gtccggtgca tggcatatga acccacgaag ggattccggg
     1921 atatcatacg gatgctcact gcaggggacg aggtggtggt tgcagggagt tacaagggtg
     1981 gcagcatcaa ccttgaaaag atcggggtga tgtccattgt tcccaggata gaacagaagg
     2041 cgccattctg ttcttcctgc aggaagcgaa tgaccagtgc cgggaaaggg aaaggataca
     2101 agtgcaggat ctgcggtgca cgggaacagg atccggaaag ggtatacctc gcaagggagc
     2161 tgaaaccggg gtggtatgag gtcgcgcctt ctgccaggcg acacctggcc aaaccgctct
     2221 gcagggggca tcctgacctt gcgaagtacg ggattggaga ttaagcggaa taatctggtc
     2281 ctgatacggc cttctcaatg attccgatct cacccggagt caactggtag agatcataaa
     2341 ccagatgatc gattgcgtgg tcgatctccc cgatttgttt ccagtatgtt gcttttattg
     2401 tcggatcttc tatttcagag atcttccgat ggagagcgat catctgcctg accagggtct
     2461 cgatcgtatc gtgaatggca acatcctccc ggttttcgaa gtcagggaca tagatgggga
     2521 tattttcaaa tgatgggccc cataatttcc ggtaaccccc cgggagtggc ggcagggtct
     2581 cattgatcaa gaaccagatc agtcgagagt tgagaagccc gagcaggtac agcgagctgg
     2641 aaatgaccat gcagcaggta tcagggcaac agtgaccggt attgtccagt gagaacctgc
     2701 tctctctcgc tatacagggg aagagaatct tcgggctggc aatgaggtcg gaatgttgcc
     2761 ctttgtccgg aagtggaaac agggtattta catccggcat cgttttctta tcagtccctt
     2821 cgtccattcg cctttccttt ctcaccattt cctttgattg gttcctcggt tggtggacca
     2881 gaaccggagg ttccttgcct atagctcctg aaggggtgag gataatatgc cattttggcg
     2941 ggaggaagta atagcgtttc agtctttcag gcgtaagaaa cggccggata atcgtggctg
     3001 attccatatc ttctgcaatc accctttttt ttgagacgga atcaataagg agtggggaat
     3061 cgtctgctcg tttgattccc cgcgaaagcg cacccatcac atactctgca agcggggtac
     3121 cttcaccctg tatcttgctg accaggtctt gtgtgcaagt atccgcaaga atccagcttc
     3181 cctgcccgat ccgccaacga ggaatgcaat atctgttttt attcagatac tccacaaagt
     3241 tcccggtagc agggataacg ccctgcacca ccgatatcga tgcccctggc tcttcctttg
     3301 cgattgtgac aatacaggtg cgcgggatga agagattctc cctttcccgg ataccaggcg
     3361 cgatgatctc ctgtattcct gatccactca ggaagtgacg aagcttggat ccatgttttg
     3421 aacgcaacca tacgtcaggg aaggcagcag aaaggagacc cccctgcttt aaaagaaata
     3481 ttcctttctc aacaaagcag ggatataatt ctccgctttg aggatagcac cggtaatgct
     3541 tctcaagata ccccttctct cccttgagtg attcacagcg gtgagcagga aaactgccga
     3601 taatcccgtc aaatcccccc gacccgatga tacgaggaaa cacactgtcc cagtcaaaga
     3661 cactgatccg ctcccttgta tcgggaccga tcagggcggt ctcaggatca tccaggaaat
     3721 ccgggccaac gagagaattt ccgcaacgga tacaggaaga gaggtccggg agcgggagtc
     3781 cctgtctatc acccccttcc atcaatgcaa caaggaggat gacctttgtt atttccaccg
     3841 ctctcctgtc aatatcaacc cccgacaaga ttgaaagcag gatccgcctt ctctcagctg
     3901 gggtcagtcg tatggtcagt tgtttgactg ggtgaatccc ctccgttccg tgaatgatga
     3961 tgaacgggag gatcgaatca gcccccttcc gttctgcccc gtcaggtagg aaaggctgga
     4021 tatcatcatg ggagaaattc ccctgtttga ggacgggcag aaggttcctg aaataccagg
     4081 cgcgatgcca ttcgaggagg aactcgaacg ccatgaccag aaacaggcct gatccacagg
     4141 caggatcgag gagatggagg tgttgaagct cctgaaccgt cttctccttt accaggtttg
     4201 ataaagttct tcgcacgaca tactccgcca tctcaaacgg aacgggagat gggtttccgt
     4261 attttcggaa ttcagaacat tcctcaatga cagcctggtg cccgttcctt atccggatca
     4321 tgctcccaag atagggggag aagacagaag caaggacccg ggcagggatg accgaaaact
     4381 cgtaagggcc tttttccggt gacaatctgg caataatgtt cttgatggta tcatcatcaa
     4441 cagagaggga atagattatt gattcggggg attccagccc acccccacgt tccgaacaac
     4501 aaaaaagacc gcccccatat tcttcacacg cgtagcggaa taacccgcac agccttccat
     4561 acgctgaacc gccaccggta atccgcatga gcaccccctc ctgcccaagc cccttatctt
     4621 ctatgatacg aaggaagagg atccgtataa gcagcgcatg caccaggtca ctgagggagt
     4681 cttccgtgac cccgtggtta tggagggcga ttttttttgc gagatgcgcc cgccatgctt
     4741 ccagatcctt ccgcagcacc tccccgatcc gtgaagtttc cctgatcccc tgtcccttta
     4801 ggaaacgccc gatcgaatcc cgtgttatac cttcacgaga gaggagggca gcgatgtggt
     4861 cccaatgctc ttcatactgg ccaataccga gtcttgtaat cagtgcacct gccacatcat
     4921 ccctgcactg caccggggtg gtggtgttat acagcgccga ttcgcggaaa ttcgttagta
     4981 tggcaagatc aagcccggca ttccaggcat atctgaggag aagaaaggca ctctcctcgg
     5041 ctggcgagca atcagcgacc ataattgcaa gggcgcctcc cttctgtgag cagaggatat
     5101 aatcgataga gccggcctgg ccgccaattc gcaccggaac atcgagcaga agcccgctgc
     5161 tttgcccaag gctgtccccg gcttgtcttt cccagccaag gctctcaagg aacggatcga
     5221 taaaatccct gcagagagtc tgtctgctga tgtccccttc acggtatgcg gggctggagt
     5281 cacgatactc ctcgatgagt ctggtgatag cgggaggtaa tggcatagtg tgagggaaag
     5341 caggatacta tctgatccag agcacagaaa agattccttt ctcccttcaa atggacgcat
     5401 agtatgcagg tgacggagcg gacgcaatta taaccgatca gacctccaag ctctaatcat
     5461 ggaatgcggg gatcctgtcg agcaggtaac catcagaccc gggatgagcg tcaacgatct
     5521 ggtgtgcgag ctcgcacggg ctggggcata taatggtggt gcgcttgccc gggctgctga
     5581 catttacgaa cggatgctga ccgataaaaa aaccgtgaaa ttcttcggcc ttgcaggggc
     5641 gatggttcct gcaggcatgg gtgggattgt cagtagtctc ctcacgcgcg gctacatcga
     5701 tctcctggtc agcaccgggg caaatctcac tcacgacatc atcgaagcac tcggatgccg
     5761 gcattaccac ggctctgcat tctgttcaga cattgagctc aggcaggagg agatcaaccg
     5821 catttacgat gtctttctcc cgaatgaggc ctttgcaacc tttgaaacgt ttatccagga
     5881 gtccctctcc acactcgatc ccggacacag ttatcccatt tccgagctcc ttgccctcct
     5941 tgggaaacga ctcgatcatg ggatccttgc cacggctgcg gcaaggggga tacccgtata
     6001 ctgtcctgca gtggcagatt cgatgatcgg cctccagtgc tggctgtact cccagtcggg
     6061 aaaaatctct gtcgatgcgt tctctgacat gaaaggcctg atcgatcgtt gttttacggc
     6121 ggaaaaagcc ggtgcactcc ttgtcggggg tggcgttcca aaaaatttca tcctccaaag
     6181 tatgctgatg actccgcagg gctttaccta tgctgtccag ctcacgggtg accggccgga
     6241 cctcgggggg ctttcgggcg caacccttga tgaggcacga tcatggggaa aaattaccgg
     6301 ggatgccctg gcagtaaccg tctatggaga tgcgactatc acgctgcctg tgctggttgc
     6361 ggcggtactg gagcggatgg aacgatgacc cagctgatcc ttgcccttga tgtcagcggg
     6421 aggaccgaag ccctccgcat cggacgccag tgtgcccccc atatcgatgc tatcaaggtt
     6481 ggatacccgc ttgtcctctc tgctggcctc accattgccc gtgacctcgc ggcaacaggc
     6541 ctgcccctta tcgctgattt caaggtcgcg gacatcccga atacaaaccg cctcatcgct
     6601 gaacaggtgt tttcatccgg attctctgcc atcatctgcc atggttttcc cggcagcgac
     6661 tccgcagagg cgtgcatcga atccgctcat gaccatgacg gcgaatgtta cctcgttgcc
     6721 gagatgagcc atccgggagc tgaggaattc ttccatgggg gtgttgccga gcacatagcg
     6781 gaacttgccg tcacgcttgg agcagacggt atcatcgccc ctgccacccg cccggatcgt
     6841 gtccgcctcc tcaggggaat cgtgcgacga aaaaagatct attcgcccgg tgtaggcgcg
     6901 cagggagggg atgctgccat agttgccggc cttgttgacg gcataatcgt cggtcggcag
     6961 atttacctct ccccagaccc gcgatctgct gccgcagaat atggtccttt ccgccagtga
     7021 tgagaagacg atgctgatcg cgtgatgagc cctatgagtc acctgaggcg aaaggacctg
     7081 ttctaaactt taaaatactg aatcgttcat tttactgtat gcaatggagc gaggaacagc
     7141 agaaactggc tgaaaagtac aagaagcttg gagatatccc cgttgccgag cggcgttaca
     7201 agtgccacac ctgtcatctt atcgtcgatg aaaagccctg cccgcagtgc ggcgatgagc
     7261 atctcgtcat catgtgcccc cttgaccact gccactgctc ccatgagatc atatcgggaa
     7321 tcg
//
LOCUS       DAOOCO010000091         7172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9772_length_7172_cov_4.916116, whole genome shotgun sequence.
ACCESSION   DAOOCO010000091 DAOOCO010000000
VERSION     DAOOCO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9772_length_7172_cov_4.916116"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PK154_06230"
     CDS             complement(<1..1183)
                     /locus_tag="PK154_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPW10694.1"
                     /translation="MTTETTGTLSKPEKIRRPRRMRALGRMIAYLGQRKALLTLVIVL
                     FFVGTVSFIFLSVMLGVAVNTLSGPSTTADLYQQVMVMAGLSLLTFISYYFGYRFLAG
                     VTQKALFQIRQDLFEHMQTLSLRFFDRQPIGELMSRVTNDIDVITAFFQQPLGTMLMG
                     LFMLVVTLAAMFLLSVTLAVVACIAIPLMMGLVWLLAKVAGPAFALLQERLADLNGLM
                     EETLAGEKTIIAFRQQDAIAGSQTVVSEGAREVGSRAQVLSLVINPLTVLVTNLDVSL
                     VALFGSVMIINHDLSVGVLTTFLSLTLLFALPLTTIFANYNFILSAAVSANRIFQIID
                     EEPDIVDKPGAVDLKPVTGHVVFRDVDFSYVPGRQILKKNSFEALPGQMIGLCGPTGA
                     GK"
     gene            complement(1180..2937)
                     /locus_tag="PK154_06235"
     CDS             complement(1180..2937)
                     /locus_tag="PK154_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10695.1"
                     /translation="MLENLRRLLVVYREVWKWIALSQVLVLFGALFMLMIPVQVSNLL
                     DNGVVAGDVEVVVESCLKMLFFAILAGAFSMANLYIAARLGEGTAHYLRKETYRKIQT
                     FSFGNLDRYPTGELLVRLTNDIYQINMAVNYATRFILYAPFMILAALVFVYIDSPDLV
                     WIFLLVIVVTALIFGAVAIMLQKQYINRQKMLDGLNNVLQEVFAGVRVVKSFVRQGYE
                     NKRFEGKNDDLRQASLPPLYYSALLLPAIFIILGLSNALVIWFGGTQVLAGTMDIGQI
                     VAFTQYFFFILAQLLILSFVLPAIISAEASAGRIVELFDATPAIADSPAPQHLDSALL
                     KGRVEFENVSFSYDGAGGKESVRNVSLVAEPGQTVAFLGPTGSGKTTLINLIPRFYDV
                     TTGLITIDGTDIRDLPQDHLRSLIGIALQESILFSGTIKENIAFSDPEIPYDHVVSAA
                     KAADADGFISAIPGGYDSRVARRGANFSGGQRQRISIARAIASRPSILILDDSTSAVD
                     VVTESRIQDALGTMMPNTTKFIVAQRISTVLTADTIILLDQGAIVAKGSHHDLIHTNP
                     LYREIFESQLGGVRREDLI"
     gene            3326..4669
                     /locus_tag="PK154_06240"
     CDS             3326..4669
                     /locus_tag="PK154_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPW10696.1"
                     /translation="MPVDEETPVIPALAESEKEDYQRLWKPGFLLAIIIIALIAIAFT
                     AVFMTLYGYLDKVIWLENDFVLSNRWTIPAGVLIFSLLVGLCQKYLHAPNVIHGSAME
                     SMRKGQAGSDYRTFPGAFLSSICSLLSGASIGPEGTIATMVSQLAIWVRTKMRIVRDS
                     HDAHLGFDMAALASAFNGIVGSPAFTGVLATEFQIGERNVFKFLIWNLVAGLVGYFFY
                     LSLGFTSFAALLPFPPLESLSAMMVVSAIILGIIGSLLAIFTGICLKGAGELMEKSFG
                     EGIVLRTVAAGIVIAVVCYFLPELLFSGEAQIHAIVQNPAAFGIGMLLMLAILKLVLF
                     ALSFKGGFLGGPVFPILFASTLVGLALSLAVPGIPVGIFVLCVEAATIAFALGAPLTA
                     IILIVVLGNPSPYLVALIVTSVATALILGALLKPIMERRIREKATPEAGQTSPCH"
     gene            complement(4833..5408)
                     /locus_tag="PK154_06245"
     CDS             complement(4833..5408)
                     /locus_tag="PK154_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW10697.1"
                     /translation="MSRDVFEEYAEDYDRWFDEHHDEYLAELSRIMKVLPAPDSRSVE
                     VGVGSGRFAAPLGIGLGIEPSRALGRMARQRGIEVVRGLAEALPLRDGSCSSILMVTV
                     ICFLEDPVPAFREIHRILVSAGFLTLAFIEDRGSIHQRYLREGGKGRFLSRARFYSQE
                     EICALLEGTGFTVTEVDARAGFCVIRAQRGY"
     gene            complement(5420..5794)
                     /locus_tag="PK154_06250"
     CDS             complement(5420..5794)
                     /locus_tag="PK154_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10698.1"
                     /translation="MRILRSVRHKVCADGSFMKEFLLDVPVTREFFTYLGNFGQVEAL
                     PNVGKGFYKFEKPDWFSIKGFAGDTSVEVRFKKEVMDLTVDFVYLLFSTYRGDGDKFD
                     LSVLKRREEAIGERVRKRLYGT"
     gene            complement(5766..6938)
                     /locus_tag="PK154_06255"
     CDS             complement(5766..6938)
                     /locus_tag="PK154_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW10699.1"
                     /translation="MLPTQKPLIYLNNAATSWPKPPGVLAAIAQSLSLPVFGSGRTTG
                     TQGEDYLTLARDGLSAFLGGAPPEHLVFTHNATDSLNILISGFLAEKKEQCHVLTTTL
                     DHNSVLRPLHEYEQQGRIRLDIIPFKDCFVSPNAVQDSIRPDTRLMVMTHGSNVLGSA
                     QDIRAIGEILHDHGIYFIVDGAQTAGHIPIGLKDLPVDAFVITGHKGLLGVPGTGGFF
                     LRDPKTIAPVRFGGTGTDSFSLLHPHGMPERFEIGTHNYPGLAALAAGVEFLNETGLA
                     IIREKGEKQTLFLIRELKKEENIMIYNDHPDLPIIAFNIKGLQNDDVGFMLARGYNII
                     SRTGLHCAPLVHKAIDGGEGCVRLSLSWFTTDEECRFAADAVREIARNADSAVGPA"
     gene            7025..>7172
                     /locus_tag="PK154_06260"
     CDS             7025..>7172
                     /locus_tag="PK154_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPW10700.1"
                     /translation="MADVTPAVLREAAEQLGIQEHASLYEIKQKYHEQIKKWHPDVSN
                     LDPAA"
BASE COUNT         1657 a         1956 c         1971 g         1588 t
ORIGIN      
        1 tcttccctgc gccggtcgga ccgcagaggc cgatcatctg gccgggcagc gcttcaaagc
       61 tgttcttttt gaggatctgc cgcccgggca catagctgaa gtccacatct ctgaacacca
      121 cgtgaccggt caccggtttc agatcgactg ctcccggttt atcgacgata tccggctcct
      181 catcgatgat ctggaagatc cggtttgcac tcactgcggc cgagaggatg aagttataat
      241 tggcaaagat ggtggtgagg gggagggcaa acaggagggt cagggagagg aacgtcgtaa
      301 ggacacccac cgaaagatca tgattgatga tcatgaccga cccgaagagc gcaacgagcg
      361 agacatccag gttcgtcaca aggaccgtga gggggttgat caccagcgag aggacctgtg
      421 ccctgcttcc gacctccctg gccccttcac tcaccacggt ctgactgccg gcaatggcat
      481 cctgctggcg gaatgcaatt atcgtcttct caccggcgag cgtctcttcc atgagcccgt
      541 tcaagtccgc gagccgttcc tggaggaggg cgaatgcagg cccggcaacc ttcgcaagca
      601 gccacacaag ccccatcata aggggaatag cgatgcaggc aacaacagcg agcgtgacac
      661 tgaggaggaa catggctgcg agggtaacaa ccagcatgaa gaggcccatc agcatagtcc
      721 cgagaggctg ctggaagaat gcggtgatca catcgatgtc gttcgtgacc ctgctcatca
      781 gctccccgat cggctgcctg tcaaagaacc gcagggaaag ggtctgcata tgctcgaaaa
      841 ggtcctgcct gatctggaac aaagctttct gggtgacgcc cgcgaggaac cggtatccga
      901 agtagtagga gatgaaggtg agtaaggaca acccagccat caccatcacc tgctggtaca
      961 gatctgccgt agtactgggt ccggaaagag tattgaccgc gaccccgagc atcaccgaaa
     1021 ggaagatgaa ggagactgta cccacgaaga acagaactat aacaagggtg agcagagcct
     1081 tccgctggcc cagatacgcg atcatccggc cgagcgctct catccttctc gggcgacgga
     1141 ttttttcagg ttttgagagg gttcctgtcg tttcggtggt catatcaggt cctccctccg
     1201 cacaccacca agttgtgatt cgaaaatttc cctgtagagc ggattggtgt ggatcaggtc
     1261 atggtgactt cccttcgcga cgatcgctcc ctgatcgagg aggataatgg tgtctgcggt
     1321 gaggacggta ctgatccgct gtgcgacgat gaatttggta gtattgggca tcatggttcc
     1381 cagcgcatcc tggatgcggc tttcggtgac aacatccacg gcactggtac tgtcatcgag
     1441 gatgagaatg gaaggccggg acgcgatcgc acgggcgatg gagatccgtt gccgctgccc
     1501 tccagaaaag ttggctcctc tccgggctac acgggaatca tagccaccgg ggatcgctga
     1561 gatgaagcca tcggcatcgg ctgcctttgc tgccgacaca acgtgatcat aggggatttc
     1621 agggtcggag aacgcgatat tctccttaat ggtcccggag aagaggatac tctcctggag
     1681 ggcgattcct atcagggaac ggaggtgatc ctgggggagg tccctgatat cggtgccatc
     1741 gatggtgatc agcccggtcg tgacgtcgta aaatcgcggg atcaggttga tgagggtggt
     1801 cttccctgaa ccggtcggac cgaggaaggc aaccgtctgc ccgggttctg cgacaagcga
     1861 gacgttcctg accgattctt tccccccggc accatcatag ctgaaggaga cattctcgaa
     1921 ttctaccctc cctttcagga gagccgaatc cagatgctgt ggggcgggac tgtccgcaat
     1981 tgcaggagtg gcatcaaaca gctccacgat tcgtccagca gacgcttctg cagagatgat
     2041 agccggcagg acgaatgaga ggatcagcaa ctgggccagg ataaagaaga aatactgggt
     2101 gaacgcgacg atctggccga tatccatggt tccggccagg acctgggtgc ctccaaacca
     2161 gatgacaagg gcattggaaa gaccgaggat gataaaaatc gcgggaagga gcagcgcact
     2221 atagtagagc gggggtaatg aagcctggcg gagatcatca ttctttcctt caaaccgctt
     2281 gttctcgtat ccctgcctta cgaaggattt taccacccgg acgcctgcaa agacctcctg
     2341 gagcacgttg ttcagcccat ccagcatctt ctgccggttg atatactgtt tctggagcat
     2401 gatcgcgaca gccccaaata tcagcgcagt aacgacgatg acgaggagaa agatccagac
     2461 cagatcgggg ctgtctatgt agacaaacac cagcgcggca aggatcatga aaggggcgta
     2521 caggataaac cgtgtggcgt agttcactgc catattgatc tggtagatgt cattggtgag
     2581 acggacaagg agttcccccg tgggataccg gtcaaggttc ccaaacgaaa acgtctgtat
     2641 tttccgatag gtttccttgc gaaggtaatg tgccgtgccc tctcccagcc gtgctgcgat
     2701 gtacaggttg gccatcgaga atgcccctgc gagaatggca aaaaagagca tcttcaggca
     2761 gctttccact accacttcaa cgtcaccggc aaccaccccg ttgtccagga ggttggacac
     2821 ctggaccggt atcatgagca tgaagagtgc cccgaagagt accaacacct gggacagggc
     2881 gatccatttc catacctccc ggtacaccac caggagacgg cgaagattct caagcatggt
     2941 cgtcagccac ccccagtctg gagcagcatt ggtatactca tgctaccctg gggtaatgag
     3001 agagggaagt atctctggtt aatcactctg aaaagttcca tggtgaattg agaagcagaa
     3061 taactattta aactaaatcc tctctgatat ggactcggtc cgatcattac agaaccatct
     3121 acatatcctt gaaactccag gcactgaagc ttagctttga aaaagggtta caattgttac
     3181 gacagctgca ataatgagaa tcacggcatt cctgtttatc ccggtccttt cccaacgttc
     3241 agagaggatc agccccctcc agatcttaat acacagagag cgatgcttat atcttaggga
     3301 aaggatgaga agatacatat cctgtatgcc tgtagatgaa gaaacccctg ttatcccggc
     3361 cctggcagaa tcggaaaaag aagactacca gcgattgtgg aaacccggat tcctccttgc
     3421 cattattatc atcgctctca ttgccatcgc ttttaccgcg gtttttatga cgctgtatgg
     3481 atacctggac aaggtgatct ggctggagaa tgattttgta ctgtccaacc ggtggactat
     3541 accagcgggg gtgctgatct tctccctcct cgttgggctc tgccagaagt acctccatgc
     3601 cccaaacgta atccacggaa gtgccatgga atccatgcgg aaaggacagg ccgggagcga
     3661 ctaccggact tttccgggag ccttcctttc gtccatctgc tcccttctct caggggcaag
     3721 catcgggcca gagggcacca tcgccacaat ggtcagccaa cttgcgatat gggtccggac
     3781 aaagatgcgg atcgtccggg attcccatga tgctcacctg ggatttgata tggcagccct
     3841 tgcctcggca ttcaacggca ttgtcgggag cccggccttt accggtgtcc ttgcgacaga
     3901 gttccagatt ggggagagga atgtgttcaa attcctgatc tggaaccttg tggcagggct
     3961 cgtaggatat ttcttctacc tctcccttgg atttacctcc tttgccgccc tgcttccttt
     4021 cccccctctt gagagccttt ctgccatgat ggtggtttct gccataatcc tcggtataat
     4081 cgggtccctt cttgcgatct ttaccggaat ctgcctgaag ggagctgggg aactcatgga
     4141 gaagagtttc ggagagggta ttgtgctccg gactgtcgct gcagggattg tgattgcagt
     4201 cgtctgttac ttcctcccgg agctgttatt ttccggtgaa gcccagatcc atgccatcgt
     4261 tcagaatcct gcggcgttcg ggatcgggat gctccttatg cttgcaatcc tcaagctggt
     4321 actcttcgcc ctgtccttca agggggggtt ccttggaggt cctgtcttcc ccatcctctt
     4381 tgcatcgacc ttggtcggcc ttgcgctctc actcgctgtc ccgggaatcc cggtcggcat
     4441 ctttgtgctc tgtgtcgagg ctgcaaccat cgcgtttgct ctcggtgctc ccttaaccgc
     4501 gatcattctt atagtagtgc tgggcaatcc ttcaccctac ctggtggcgc ttatcgtaac
     4561 ctccgtggcc accgccctga tccttggggc tctgttgaaa ccgatcatgg aacggcggat
     4621 acgcgaaaaa gccacaccag aagcaggcca gacttcaccg tgccattgac atgtcctcgg
     4681 gaagcgctga aaccagcagt cgcccaggct gaaacggcct agccctggat tgttgaacta
     4741 tggtaaatcg gcgataattg ctctcgcgga gaaacggggt tgaagaatga caaaactgat
     4801 ttttttttgt cactactgga gcgcgaacaa tctcaataac cccgctgtgc cctaatcaca
     4861 cagaaaccag cccttgcatc tacctccgtc acggtaaacc cggtcccctc aaggagtgca
     4921 cagatttctt cctgcgaata aaaccgcgct cgggagagga accgcccttt tccgccttcc
     4981 cgaaggtatc tctgatggat agatcccctg tcttcaatga atgcgagggt gagaaatccg
     5041 gcggacacga gaatgcggtg gatttccctg aatgcgggaa ccggatcttc aaggaaacag
     5101 ataacagtta ccatgagtat cgaagaacat gagccatccc gcagtgggag cgcctcggcc
     5161 aagccccgga cgacttcaat ccctctctga cgtgccatcc gaccgagggc acgagagggt
     5221 tcgatcccca gtccaatccc caggggggcg gcgaaacggc cggagccgac ccccacttcg
     5281 actgagcggg agtctggtgc cgggagcacc ttcatgatgc gggagagttc tgcgagatac
     5341 tcatcgtggt gctcatcaaa ccaccggtca taatcttctg catactcctc aaatacatcc
     5401 cgcgacactg atgcacctct cacgtcccgt agagacgctt ccggacccgt tctccgatgg
     5461 cctcctcccg acgtttcagg acagagaggt cgaacttatc cccgtctcct ctgtaggtag
     5521 aaaagaggag atagacgaaa tcgacggtca ggtccatcac ctccttctta aacctgactt
     5581 ctaccgaggt atcgccggcg aaccccttga tggaaaacca gtcaggcttc tcgaacttgt
     5641 agaatccctt gccgacattc gggagtgctt caacctgccc gaagttcccc agataggtaa
     5701 aaaattccct tgtaaccggt acgtcgagca ggaactcctt catgaaggag ccgtcagcac
     5761 agaccttatg ccggaccgac cgcagaatcc gcattcctgg ctatctcccg cacggcatca
     5821 gccgcgaacc ggcattcttc atcggtcgtg aaccaggaga ggctgagccg gacgcagcct
     5881 tcgcctccat caatagcctt gtgcaccagg ggggcacagt ggagcccagt ccgagagatg
     5941 atgttgtatc ccctggccag catgaatccc acgtcatcgt tctggaggcc cttgatattg
     6001 aacgcgatga tgggcaggtc cgggtggtca ttgtagatca tgatattttc ctccttcttc
     6061 agttccctga tgagaaacaa tgtttgtttt tccccctttt ccctgatgat tgccagtccc
     6121 gtctcgttaa ggaactccac tcccgctgca agcgctgcca ggcccgggta gttgtgggtc
     6181 ccgatctcga accgctccgg catcccatgg ggatggagga gcgagaacga gtccgtcccg
     6241 gtcccgccaa accggaccgg tgcaatggtt ttcgggtcac gcaggaaaaa tccccccgtc
     6301 ccaggaacgc caaggagacc cttgtggccg gtgataacga aggcatcgac cggaagatct
     6361 tttaggccga tgggaatatg gccggcagtc tgggcaccgt ccacgataaa gtagatacca
     6421 tgatcatgca ggatttcccc gattgccctg atatcctggg ccgatccgag cacattgctg
     6481 ccatgggtca tgaccatgag gcgggtgtcg ggacggatgg aatcttgaac ggcgttggga
     6541 ctgacaaaac agtccttgaa gggaatgatg tccaaccgta tgcggccttg ttgctcatat
     6601 tcatgcaggg gacggagcac ggagttgtgg tccagtgtcg tggtaaggac atggcattgc
     6661 tctttttttt ccgcaagaaa accagatatg aggatattga gggaatccgt tgcattatgg
     6721 gtgaacacaa ggtgttcggg cggtgctcct cccagaaacg cggaaagccc gtcccgggcc
     6781 agtgtgaggt agtcctcacc ctgggtccct gtcgttctcc cggagccgaa gaccgggagg
     6841 gagagggatt gcgcaatcgc tgccagtacc ccaggaggtt tcggccagct ggttgctgca
     6901 ttgttgagat aaataagtgg tttttgagtg gggagcatct ttttttaaga tctgataatc
     6961 accgggaaaa agggatcggt agtaccacga accattatcg cgggatgagc ggaacaaata
     7021 gtacatggcc gatgtcactc ctgcggttct cagggaagca gcagagcaac tgggaatcca
     7081 ggaacatgcc tccctctatg agatcaagca gaaatatcat gaacagatca agaaatggca
     7141 tcctgatgtc tcaaatctcg atccggcagc ct
//
LOCUS       DAOOCO010000092         7146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9820_length_7146_cov_5.296714, whole genome shotgun sequence.
ACCESSION   DAOOCO010000092 DAOOCO010000000
VERSION     DAOOCO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9820_length_7146_cov_5.296714"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK154_06265"
     CDS             complement(<1..305)
                     /locus_tag="PK154_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HPW10701.1"
                     /translation="MTMKVLVVGGGGREHAIACALSRSEEVVLLSVMPKHNPGIASLS
                     SDVLIEKETNIPAVVDFAVSRGADYAVIGPEAPLEAGIVDRLEKRGIPCIGPILKAA"
     gene            complement(327..863)
                     /gene="pyrE"
                     /locus_tag="PK154_06270"
     CDS             complement(327..863)
                     /gene="pyrE"
                     /locus_tag="PK154_06270"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPW10702.1"
                     /translation="MVKSIAHLLKENGAVEYGEFVLASGVKSSYYLDVKSAIANPAVL
                     KVIGREIKKIASFDVVAGVAVGGVPIAVATALASGKPYAIIRAGEKLHGKGGQIIGKV
                     GGLAVLLVEDVTTSGGSVIRGIEALRAEGAFVDTVVTVVDREAGARDALAERGVHLHA
                     LCRAREIVGDERAIDPIF"
     gene            complement(857..1357)
                     /locus_tag="PK154_06275"
     CDS             complement(857..1357)
                     /locus_tag="PK154_06275"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HPW10703.1"
                     /translation="MMPAYVPNPVAAFVGGGTPIDGGRHFRDGRRIFGDGKTWRGFFA
                     GVAAGIIMGMLQILAYDAAGITRLPELTLPVVCLLSFGALLGDLVKSFFKRRLGKDRG
                     APWPIADQYDLVIGPFVLILMFEYTWVVSYITLPILFWIIIITPLLHRAVNIIGYIAG
                     IKDVPW"
     gene            1500..2954
                     /locus_tag="PK154_06280"
     CDS             1500..2954
                     /locus_tag="PK154_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10704.1"
                     /translation="MMIKETKTLCPICKAILDATIEEEGGSIWLVRSCPEHGHFRDLY
                     WSDAALWHKFEQFDSVGRGVENPNVVNPGGSCPENCGLCSFHKSGTLLANIDLTNRCN
                     LDCDFCFANARACGFIYEPSFDEIVKMMQLLRAEKPVPAPAVQFSGGEPTMRDDLMEL
                     IRKAKEIGFPQVQLATNGIKMAQDVGYVEDLKSAGLSTVYLHFDGVTKETNSKIKSDE
                     KAVRNCEDVGLGVVLVPTIIKGRNDHEIGPIIRYAADHFKAVRGVNFQPIAFTGAASE
                     EDVQRERITIPELLEDIEDQTSGIIRKSDFYPVPCVVPFSDLVEAYTGLPQVRFTPHQ
                     HCGAATYVFITDKGMVPINRMVDVESFFRSIENLTEKLKKGGQLNKYLSLIEGIREMN
                     DSFRKSEQGSTAQFWKLIGKTLVMQNFDALREFHWNALFVGTMHFMDKYNYDLSRVQR
                     CCIHYATPDGKLIPFCTYNSGPVYREKVWSAHRK"
     gene            complement(3096..4031)
                     /locus_tag="PK154_06285"
     CDS             complement(3096..4031)
                     /locus_tag="PK154_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330490.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPW10705.1"
                     /translation="MFYYQNPAEGLDIREVNRVVEEAFAEHGRGRVQMPPKLYVTFDH
                     GDFRTMPAYIPSLSLAGVKIVNVHPGNPRIGFPTVMALLIILDPGTGIPVAILNATTL
                     TDLRTGAAGGVAAKYLAPSRTVTLGLVGSGRQAAAQLDAIAAEREVTEIKVWSRNEKN
                     AVAFCRKYSSLDCTVAPLEKVCDSDVLVTSTPSREPVIRDEWIREGTHINAIGADAPG
                     KQELDPGILQRARVFVDDPAQAVHSGEINVPIAKGLFSPERIEGTLGEVVLGRKKRMS
                     QDEITLFDSTGLAIQDLAVAEIAMKSRERVALRFP"
     gene            4147..4941
                     /locus_tag="PK154_06290"
     CDS             4147..4941
                     /locus_tag="PK154_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPW10706.1"
                     /translation="MIHIIPKPTDTTDDNSTAAVAGQIRSQGGTCELIDIHRIDPFMP
                     PFEHEVVWVCGLTQDEHQFEVLQALSLSNVLVNTPQAIGICASKVFTTALLMRHSVPS
                     PRTLFTSSKEIASRFIQEHGRVVIKPIYGFDGHGIFLIDAPGQLGNPPYYLQEYVPND
                     RDFRIFVIDGEPVGAIMRVSDTLAHNIHQGGTGIPSEIDDEMRSIAAAAAQATGVDYG
                     GVDLLRYHDSYCVLEVNGTPNWHCMAAPIPALLARYLLEKERDLKA"
     gene            complement(5000..5284)
                     /locus_tag="PK154_06295"
     CDS             complement(5000..5284)
                     /locus_tag="PK154_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HPW10707.1"
                     /translation="MHKEELISLHQMLYEIKDYFEGSNPDLKFSEYHSLKINPSQLHK
                     SKMEHKYAIFVLGNELANAMKEVEFSASGRISARMRELAAKTLKEMEYIQ"
     gene            complement(5343..6575)
                     /gene="dnaG"
                     /locus_tag="PK154_06300"
     CDS             complement(5343..6575)
                     /gene="dnaG"
                     /locus_tag="PK154_06300"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HPW10708.1"
                     /translation="MYSSDTTKYLIHLHLEAEGVVEKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQISSKKGETKGEIYIASSLDRAETAILAASLETIDRVGPCVARV
                     NVVTVEDIRVSKRKKIVERAKELLIDSFEEGSIDSTEIIDSVRESLRIEKIGNIGGDQ
                     VPAGPNVLESDAIIVVEGRADVINLLRYGIKNAVGVEGTKISPTIIELCEKKTATAFF
                     DGDRGGDLLLQELLQVAEVDYVAYAPRGKSVEDLSRKEIIKALRNKVPVEYVKEQVPE
                     EVPAGESEEPLRPNSRKEGIKDEREEKRKVPLKKKQDAGEKKPKTLGDQINAVRGKRI
                     ARFLSSDLSVLKESPSDEIEKEFTDLNGDVAGLVTDGEVNQRILDLVAGKGIEYIAAR
                     EFNGIIKRPLTIRLIKIS"
     gene            complement(6712..7023)
                     /locus_tag="PK154_06305"
     CDS             complement(6712..7023)
                     /locus_tag="PK154_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPW10709.1"
                     /translation="MTDITPALSCSDKGTIITIEVAAGSKSSCFPSGFNEWRKAVGCR
                     VRAPAVEGRANREVVTVIAGAIGIEHSRVRIISGSTAPVKKILIEGMTPDQLRSVLTL
                     S"
BASE COUNT         1637 a         2051 c         1818 g         1640 t
ORIGIN      
        1 atggctgctt tcaggatggg cccgatgcag gggatacctc tcttctcgag gcgatcgaca
       61 atccccgcct cgagcggggc ttccggtccg atgacggcat aatcggcacc ccgcgagacc
      121 gcaaaatcga caactgccgg gatattggtc tccttctcaa taaggacatc agaagagagc
      181 gaggcaatcc cgggattgtg cttcggcatt accgagagca ggaccacctc ttccgaacgg
      241 gagagcgcac atgcaatcgc atgttccctc ccccccccac cgaccaccag caccttcata
      301 gtcatctata atacgacacg caattcttaa aaaattggat ctattgctct ctcatcaccc
      361 actatctcac gtgcacggca gagggcgtgc agatgtacgc cccgctctgc aagcgcatcc
      421 cgggctccgg cttcccgatc gacaacagtg accacggtat ccacgaaagc cccttcagcc
      481 cggagtgcct caatccccct gataacggat ccccctgacg ttgtcacatc ctccacgagg
      541 agcaccgcga gaccgccgac ttttccgatg atctgcccgc cttttccatg cagcttctcg
      601 ccagccctga tgatggcgta cggcttcccg cttgcaagcg ccgtggcaac cgcgataggg
      661 actcccccaa cagcaacccc ggccaccaca tcgaacgagg cgatcttttt gatctccctg
      721 ccgatcactt tcagcacggc aggattggca atggccgact tcacatcgag gtaatatgaa
      781 ctcttcacgc cggaagcgag aacaaactcg ccatactcga cggccccgtt ctccttcaac
      841 agatgagcaa ttgacttcac catggtacat cctttatccc ggcaatatac ccgataatgt
      901 tcaccgccct gtggaggagc ggggtgatga tgatgatcca gaagaggatc gggagggtga
      961 tgtatgatac cacccacgtg tattcaaaca tgaggatgag gacaaacgga ccgatcacca
     1021 gatcatattg atcagcgatc ggccagggtg caccccgatc cttccccagt cgccgcttaa
     1081 agaaactttt taccagatcc ccgagaagcg ccccgaacga gaggaggcag acaacaggga
     1141 gggttagttc cgggaggcgg gtgatccctg cagcatcata ggcaaggatc tggagcatcc
     1201 ccataatgat ccccgccgct accccggcaa aaaaacccct ccaggtcttt ccatccccga
     1261 atatccgccg cccgtcgcga aaatgcctcc cgccgtcgat tggtgttccc cccccaacaa
     1321 aggccgcgac cgggttaggc acataagccg gcatcatgat ccagagcgct gacaggagcg
     1381 ggacaagaaa ggaatcagga ctaatcgcta atctcccact atctgtaata aacagcagat
     1441 ataaacatta agatatccca caggtaacaa cttatgagac aggatcggtg gggatcagga
     1501 tgatgatcaa ggaaacaaaa accctttgcc cgatctgcaa agcaattctc gatgcaacaa
     1561 ttgaggaaga gggtggaagc atctggctgg tgcgatcatg cccggagcat gggcattttc
     1621 gtgacctgta ctggtctgat gcagcgctat ggcataagtt cgagcagttt gactccgtgg
     1681 ggaggggcgt tgagaacccg aatgtggtca atcccggcgg ttcctgcccg gagaactgcg
     1741 gcctctgcag ttttcataaa tccgggacgc tgctcgccaa tatcgatctg acaaaccggt
     1801 gtaacctcga ctgcgacttc tgttttgcca acgcccgggc atgcggattc atctacgaac
     1861 cttcttttga cgagatcgtc aagatgatgc agctgctccg ggccgaaaaa ccagtcccgg
     1921 cgcctgcagt ccagttctcg ggaggggaac cgaccatgag ggatgacctg atggagctga
     1981 tccgaaaggc aaaggagatc ggatttcccc aggtccagct ggcaacgaac gggatcaaga
     2041 tggcgcagga tgtcgggtat gtcgaagatc tcaaaagtgc aggactctcc accgtatatc
     2101 tccattttga cggggtgacc aaagagacca actccaagat caaatccgat gaaaaagcgg
     2161 tcaggaactg cgaggatgtc ggtcttggcg tggtgcttgt gccaactatt atcaagggaa
     2221 ggaacgatca cgagattggc ccgatcatcc gttatgctgc agaccatttc aaagcggtac
     2281 ggggggtcaa tttccagccg atcgcgttca ccggagcagc cagcgaggag gacgtgcaga
     2341 gggaacggat aaccatcccc gagcttcttg aggatatcga agaccagacc agcggcatca
     2401 tcaggaaatc agatttttac cctgttccct gcgtcgttcc cttctctgac cttgtcgagg
     2461 catataccgg gctgccgcag gtcaggttca ctccccacca gcactgcggt gctgccacct
     2521 atgtgttcat caccgacaag ggaatggttc ccatcaaccg gatggtcgat gtcgagagtt
     2581 tcttcaggtc gatagagaac ctgaccgaga agctgaagaa gggcgggcag ttgaacaagt
     2641 acctgtccct gatagaagga atccgggaga tgaatgattc attcaggaag agtgaacagg
     2701 gaagtaccgc ccagttctgg aaactgatcg gaaagaccct cgtgatgcag aactttgatg
     2761 ccctgcgtga attccactgg aatgccctct ttgtcgggac gatgcatttc atggacaagt
     2821 ataattacga cctctccagg gtacaacgct gctgcatcca ctatgccacc cccgatggga
     2881 agctgatccc gttctgcacc tacaacagcg gcccggtcta ccgggaaaaa gtgtggagtg
     2941 cccaccggaa ataaagcctt tttccccttt tttatatgaa aatgcctccg gcactttcat
     3001 cttttatgct tgtggccctc aaaggaatca tattattttc ttctcctctt gcacgagggc
     3061 ggatttccgg agaccggttc ccagggatcc cgccatcatg gaaacctgag ggctacccgt
     3121 tcgcgactct tcatggcaat ctctgcaact gccaggtcct ggatggccag tccggtcgag
     3181 tcaaagagcg ttatttcatc ctgactcatc cgtttctttc tgccaaggac cacttcaccc
     3241 agcgttcctt caatcctctc cggtgaaaag aggccttttg ctatgggcac attgatctcg
     3301 cccgagtgaa ctgcctgggc ggggtcatcg acaaaaaccc tggcacgctg gaggatgccc
     3361 ggatcgagct cctgtttccc gggagcgtcg gcaccgatag cgttgatatg ggtcccttca
     3421 cggatccatt cgtcccggat gaccggctcg cgggaaggtg tcgaggtaac aaggacgtcg
     3481 gagtcacata ctttttcgag cggggcaaca gtgcagtcaa ggctgctgta cttcctgcag
     3541 aatgcaactg cattcttctc gttcctgctc cagaccttta tctctgtcac ctcgcgttcg
     3601 gctgcaatgg catcgagctg ggctgcagcc tggcggccgc tgccgaccag ccccagcgta
     3661 acggtgcgtg acggagcaag atattttgcc gcaactcccc ctgcagcccc tgtcctcagg
     3721 tcagtgagtg tcgttgcatt caggatggca acgggtatcc ccgtccctgg atcaaggatg
     3781 atcaggaggg ccatcaccgt tggaaatcct atccggggat ttccgggatg tacattcacg
     3841 atcttgacac cggcgaggga gagtgagggg atgtacgcgg gcatcgtcct gaaatcacca
     3901 tggtcaaaag tgacatagag cttgggaggc atctggacgc gtccgcggcc atgctcggca
     3961 aaggcctcct ccaccacccg gttcacctcg cgaatatcca gcccttcggc agggttctgg
     4021 taataaaaca tactactcac tctcccttcc cggaagaaga agatactcac cctgcgcccg
     4081 ttccgcggag cttatcccct ttcccggacc accctgtacc tgcaagagaa ggaggcgtgg
     4141 gagatcatga tccatattat cccaaaacca actgacacaa cggatgacaa ctcgaccgct
     4201 gctgttgcag gacagatcag gagccagggc gggacctgtg agctgataga catccaccgg
     4261 attgatccgt tcatgcctcc tttcgaacac gaggtcgtct gggtatgcgg gcttacccag
     4321 gatgaacacc agttcgaggt gctccaggca ctctccctct caaatgtcct ggtgaatacc
     4381 ccccaggcga tagggatctg cgcaagcaag gtgttcacca ccgccctcct catgcgccat
     4441 tcggttcctt ctcctcggac cctttttacc agctcgaagg agattgcaag ccgtttcatc
     4501 caggaacacg ggagggtggt cataaaaccg atatatggat ttgatggcca cgggatcttt
     4561 ctcattgatg ctcccggaca actggggaat cccccttact atctccagga gtatgtcccc
     4621 aacgaccggg acttccggat attcgtcatc gacggtgagc cggtcggggc catcatgcgg
     4681 gtatccgata cccttgcgca taatatccac cagggcggca ccggaattcc cagtgagatc
     4741 gacgacgaga tgagatcaat tgcagcggcg gcagcacagg cgaccggtgt cgactacggg
     4801 ggtgttgatc tgctccggta tcacgacagc tattgtgtcc ttgaagtgaa tggcactccg
     4861 aactggcact gcatggctgc acccatccct gcgctccttg cacggtacct cctcgaaaaa
     4921 gagcgtgacc tgaaggcctg aagaacagtt ctgaggaaat aaaacgggaa gaaaaacgaa
     4981 agaaaccaaa aacctgaagt tactggatat attccatctc tttcaatgtc tttgcggcaa
     5041 gttccctcat ccgggcagat atccgccctg atgcagagaa ttccacttcc ttcatcgcat
     5101 tggcaagttc gttcccgagg acgaaaatgg catatttatg ctccatcttg cttttatgca
     5161 gctgcgatgg attaattttc agggagtgat attcggaaaa tttcaggtct ggattggatc
     5221 cttcgaagta gtccttgatc tcatagagca tctgatgcaa tgatatcagt tcctccttat
     5281 gcacacgctc acccgtctta gtcttctctc ccggagaaat atataagtag cgatgaaaaa
     5341 aatcaggaga ttttaatgag cctgatcgtc aggggacgct tgataatccc gttgaattcc
     5401 ctggccgcga tatattcaat ccctttgccg gcaacgagat caaggatccg ctggttgacc
     5461 tcgccatcgg tgacgagacc tgcaacatct ccgttgagat cggtaaattc cttctcaatc
     5521 tcatcggaag gcgattcttt cagtactgag agatccgagg agagaaatcg tgctatcctc
     5581 tttcccctga ccgcattgat ctgatcaccg agggttttcg gttttttctc cccggcgtcc
     5641 tgctttttct tcagcggcac cttccgcttc tcttcccgtt catccttaat accctctttt
     5701 cgggaattgg gacggagcgg ctcttcactc tccccggcag gtacctcttc aggcacctgt
     5761 tctttgacgt actccaccgg gaccttgttt cgcagtgcct tgataatctc ttttctggat
     5821 aggtcttcta cgctctttcc ccgtggggcg taggcgacgt aatcgacctc tgcgacctgg
     5881 aggagttcct ggaggaggag atcccccccc cggtccccgt caaagaatgc agtagcagtc
     5941 ttcttctcac agagctcgat aatggtcggg gaaatcttgg tcccctcgac tccaaccgca
     6001 tttttgatgc catacctgag caggttgatc acatcggccc gcccttccac cacaataatg
     6061 gcatccgact ccaggacgtt gggacctgcc gggacctggt ccccgccgat gttgcctatc
     6121 ttctcgatcc ggaggctctc cctgacacta tcgatgatct cggtgctgtc gatcgatccc
     6181 tcctcaaaag agtcgatcag aagttccttt gcccgttcaa cgatcttctt ccgtttgctg
     6241 acccggatat cctctacagt gaccacattg acgcgggcga cacaggggcc tacccggtcg
     6301 atggtctcaa gggatgctgc caggatcgcc gtttcagccc tgtcaaggga tgacgcgata
     6361 tatatttcgc cttttgtttc gcctttttta ctggagatct ggacatcgat ccttccgaca
     6421 cggccggtcc tctggagatc gcgaaggtcg agatcctcac cgagcaaccc ttcagtctgg
     6481 ccaaatatgg caccgactac atcgggtttc tcgaccaccc cctctgcttc gaggtgaaga
     6541 tgaataaggt acttggtagt atctgatgaa tacacgtaag cgcaccccca tgcatcatgc
     6601 aatcggtgta atgcctgatc ataggtaaac catagttgct cccgggaagg gataaactat
     6661 atgtttattg ttaatatttg atgatttatt tatttattag tatacaatat gtcaggagag
     6721 tgtcagcacc gatctgagct gatccggggt catcccttca atgagtatct tttttaccgg
     6781 cgcggtgctt ccggaaatga tgcgtacccg tgaatgctcg atacctatcg ccccggcgat
     6841 taccgtgact acttctctgt ttgcccgccc ttccactgcg ggtgccctga ccctgcatcc
     6901 caccgccttt ctccattcat tgaacccgga aggaaaacag gagctctttg aaccggcggc
     6961 aacttcgatc gtgataatgg tgcctttgtc agaacaggag agagcagggg tgatatcggt
     7021 catgagccat cagaagtatg atgtcgcccg gcactaacaa caccggatca ccagtggcat
     7081 gctgccgttc ttcaccatgt ttttcccatt gccgggcggt gcctgtcgcg caaccgggtt
     7141 gtcccg
//
LOCUS       DAOOCO010000093         7140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9831_length_7140_cov_6.207904, whole genome shotgun sequence.
ACCESSION   DAOOCO010000093 DAOOCO010000000
VERSION     DAOOCO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9831_length_7140_cov_6.207904"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PK154_06310"
     CDS             <1..845
                     /locus_tag="PK154_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW10710.1"
                     /translation="WGLYAGCIYILLATGLNLIFGVMKIVNFAHGELLMLGAYITFTF
                     YAVSGFNPYVLLAASLPILILIGVIIERTCFRPILGTGKLNEIFISIGLIYIIQNAAA
                     MIWSDEWRTIHSPYEHITLKMFGVNLPLDYLIIVITTISILIILFLFMQRTRLGKAMR
                     ATSQNRKGAMLMGINVERMDMLAFGIGAGLAGAAGTLWVVSGQVFNPYMGSIPAVKAF
                     AIVIIGGLGSIPGAIIGGLIYGIAENFAILTLGGAWKDAISFIILILVLIVRPTGIFG
                     ESAE"
     gene            842..1834
                     /locus_tag="PK154_06315"
     CDS             842..1834
                     /locus_tag="PK154_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW10711.1"
                     /translation="MIDMNKTLGIGSKRARLLGVILLLLLAVTLPLIFGMNLYLLTVL
                     VLMVIFIIYASSWNFLTYTGQGSLGHAAFFGLGGYASAIVATASGLSPYITIFIGGGL
                     AALVGLFIGIICVRLKEWFLAMVTFGFAIIIQTLMVSQFADISGGWDGIAAPRLVPSS
                     IPNYQLIEYYSIVLIAVFVILLFYFILKSKIGLAFAAIRENEIEARAAGINPVKYRLF
                     AFLVSAYFSGVAGALEIHNLGYITPEIFAVDISFWPIIYSISGGLGTLAGPIVGTIFI
                     TLIWDGLQSFGLTYLRFVIIGLLLVLIIIFLPKGLISLSDKIKGYLGKRQEKAQ"
     gene            complement(2136..2852)
                     /locus_tag="PK154_06320"
     CDS             complement(2136..2852)
                     /locus_tag="PK154_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10712.1"
                     /translation="MNRAESPLLEIRQLSVFYSGLQVLWNVSLFINQGEIVTLLGANG
                     AGKTTLVQSVFGLTKDRKGQIFFEGTEITGLPAFEMVKRGLALVPEKRELFPKMTVEE
                     NLELGGFSKREGVVAPEEIFKFFPILKERKKQLAGTLSGGEQQMLAIARALIPKPSML
                     VLDEPSLGLSPLIVSSVLDAVKKLNQEGLSILLVEQNVQHALEMSHRGYILETGRIVK
                     EGSSSDLLHDDQVQAAYLGT"
     gene            complement(2849..3574)
                     /locus_tag="PK154_06325"
     CDS             complement(2849..3574)
                     /locus_tag="PK154_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10713.1"
                     /translation="MLKLEGVTKKFGGLVAVNGIDLDIHEGEILGLVGPNGAGKTTLL
                     NIISGIYRPDKGSVQYLGEEISRLSMDKICRRGIAKTFQHTLSFPGLTARESVMIGSL
                     FGNGHKATMSGAAKEAEELLGRIGFPLERIDMPLSSLNVIELRRTQLARALASRPKLI
                     LLDEMMTGLNPNESTEAVSLIKSIRDDGITIVMIEHVMRVILGVSDRMVVLDRGEKIA
                     EGIPDEVVKNQRVIDSYLGERYI"
     gene            3867..4478
                     /locus_tag="PK154_06330"
     CDS             3867..4478
                     /locus_tag="PK154_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10714.1"
                     /translation="MPEEIRDLIEKINQEGIKAAEEKANIIEVEARQRADAILSQAQA
                     EADRMIAAADQRIMREDERERALLAQAGRDLLLSLRQEINVMLGRIVVSDIREALTPE
                     LLYRLLSEIIRNYPKMERGNIIISLKKNDLDLLEQQYLQKLQLEAARGITLQPGEEIQ
                     GGFTISFDSGKSCYDFTDKALAGYIITYLKPKLKRILEGSIEE"
     gene            4485..5024
                     /locus_tag="PK154_06335"
     CDS             4485..5024
                     /locus_tag="PK154_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPW10715.1"
                     /translation="MGEFYPYLIASLPTLHFGMKPSFSFEQFLETCHRLIPEKDFQIL
                     SNLPRPENYSEEGRKHKFVERWIKFDTALRNETVRMRAGRRHIEAATYLRPDTYPGPS
                     LAPVVAAATMSPSLLEGEKVLDETRWKMLDELANAHYFDLDALITYAYKLLILHRWER
                     IRNADAALLLREALEQEAV"
     gene            5025..6770
                     /locus_tag="PK154_06340"
     CDS             5025..6770
                     /locus_tag="PK154_06340"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPW10716.1"
                     /translation="MTALSSGRIVRINGNMVTVTFDTPVMQNEVAFIRHSEERLKSEV
                     IRIRGNLAEMQVYEATTGLKIGEEVEFTGELLSIELGPGMLGQIYDGLQNPLPEIAEQ
                     CGFFLQRGVYINALSESKTWDFTPEKKPGDRVRAGDKIGYVEEKIFRHYCMVPFTLTG
                     NYEIISIENAGRFTIRQAVAQIMDQNGKIYPVFLSQTWPVKIPIQAYTERLKPEEPLV
                     TKMRLIDSLFPVSRGGTYCIPGPFGAGKTVLQQLISRHAEVDIVIIAACGERAGEIVE
                     TLRTFPSIIDPRTSRPLSDRTVIICNTSSMPVAARESSVYTAVTIAEYYRQMGLHVLL
                     LADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESRIASFYERAGVVRLHDGSLGSVTI
                     GGAVSPAGGNFEEPVTQATLKVVGAFLGLSRSRSDARRYPAIDPLISWSKYRSIIDER
                     QRERGQGILLKGNDIAQMMKVVGEEGTSLQDYMDFLKAEFFDFVYLQQNAFDPVDEAT
                     MKDRQIYIFDFIHRIIESEFVFERKDEALHFFQQLRQLFRGWNSISWQSNEFKKTEKE
                     IRAFLLVRTREGKHA"
     gene            6763..>7140
                     /locus_tag="PK154_06345"
     CDS             6763..>7140
                     /locus_tag="PK154_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10717.1"
                     /translation="MHKIYTNINQIVGDVIAVDAENVGNTELAEVTTRRGTSLAQVIR
                     LDGNKAFLQVFAGSRGISTGDRVRFLGHPMRIATGDLLLGRVFNGSGRPLDNGPDLSE
                     NLVDIGGPPVNPVKRIIPNKMIRT"
BASE COUNT         1771 a         1758 c         1790 g         1821 t
ORIGIN      
        1 tctggggatt gtatgccgga tgcatttata ttcttctcgc caccggcctg aacctgatct
       61 tcggcgtgat gaagattgtc aattttgccc atggcgaact gctcatgctt ggtgcgtaca
      121 tcacgtttac cttttacgcc gtctccggat tcaaccccta cgtgctgttg gcggcctcgc
      181 tccccatcct gatccttatt ggagttatca tcgaacgcac ctgtttccgc ccgatccttg
      241 gtactggaaa actgaacgag atctttattt ccattggtct tatttacatc atccagaatg
      301 cagcggcgat gatatggtct gatgagtggc ggaccatcca cagcccctat gaacacataa
      361 ctctgaagat gttcggggtg aatctgcctc tcgattacct catcatcgtt atcactacca
      421 tctcaatcct gatcatcctc ttcctcttta tgcagaggac ccggttgggg aaagcgatga
      481 gggcgaccag ccagaacagg aagggtgcaa tgctgatggg gatcaatgtc gagaggatgg
      541 acatgctagc ctttgggatc ggtgccggcc ttgccggcgc tgcagggacc ctctgggtag
      601 tgagcggcca ggtcttcaat ccgtatatgg gatcaatccc agcggtcaaa gctttcgcta
      661 tcgtgattat tggaggtctg gggagcatcc ctggtgccat aattggggga ctcatctatg
      721 gaatcgccga gaactttgca attctcaccc ttggcggggc atggaaagat gcaatctcgt
      781 tcataatcct gatccttgtt ctcatcgtga ggcctaccgg catcttcggg gagtcggcag
      841 aatgatcgat atgaacaaga cccttggcat cgggtcgaaa agggccaggc tgctcggcgt
      901 tatcctcctc cttttacttg ctgtcaccct gccgctcatc ttcgggatga acctctatct
      961 tcttaccgtc ctcgtcctga tggttatctt cattatctat gcatcctcct ggaatttcct
     1021 tacctatacc gggcagggat cgcttggtca tgccgccttc tttggacttg gggggtatgc
     1081 ctctgcaata gttgcaacgg cgagcggcct ctcgccctat atcacgatct ttattggcgg
     1141 aggcttggca gctctggtcg gactcttcat tggtattatc tgtgtcaggc tgaaagaatg
     1201 gttcctcgcc atggtgacgt tcggctttgc cattattatt cagaccctta tggtaagtca
     1261 attcgccgat atcagtggag gctgggatgg aatcgctgca ccaagacttg ttccctcaag
     1321 cattcccaat tatcagctta tcgaatacta ctccatcgtc cttatcgcgg tctttgtgat
     1381 cctgctcttc tatttcattc tgaagtcaaa gataggtctt gcgtttgccg ctataaggga
     1441 aaacgagata gaagcccggg ctgcagggat caatccggtc aagtaccgtt tatttgcctt
     1501 tttggttagt gcatactttt cgggagtggc aggagcactt gagatccata acctgggata
     1561 catcactccg gaaatttttg ccgtggatat ctcgttttgg ccgatcatct actcgatatc
     1621 cggcgggctg gggacccttg ccggccctat agtgggaacc atatttatta ccctcatctg
     1681 ggacggtctc cagtcatttg gactcaccta tcttcggttc gtgatcatcg ggcttcttct
     1741 ggtcctgatc atcatcttcc tgccgaaagg gctcatctca ctgtctgaca agatcaaagg
     1801 atacctagga aaaaggcaag aaaaagccca atagacttac ttactctttt tccaatgaaa
     1861 atcaattcgt aattttcata atttatgccc gtatcccgaa atcggggctc ataggaggtt
     1921 tatatgaaaa tgcctccgca ttttcatctt ttatacttgt ggccctcgaa ggaatcatgt
     1981 ttttttaaaa accaatcagc gccaacggaa gggtgttttc ctttttgatc cccggctcat
     2041 cgaaagcagt tcaacagaac gatatgatga ggctacgatc gttttccctc tattcggcaa
     2101 atatgagcga agtggagaag aggtggcttc ccccgttaag tcccgaggta tgctgcctga
     2161 acctggtcat catgaagaag gtcagaggac gatccctcct tcacaattct ccctgtctca
     2221 agaatatatc cacgatggga catttccaat gcatgctgaa cattctgctc gacaagcaga
     2281 atagacagac cttcctggtt cagcttcttg actgcatcga gcaccgaaga tacaataagg
     2341 ggagaaaggc cgagagacgg ttcgtcaagt acaagcatgg acggtttggg aatgagggcg
     2401 cgggcgattg ccagcatctg ctgttcccct ccggaaaggg ttccagccag ctgtttcttc
     2461 cgttccttga gaatgggaaa gaacttaaaa atctcttccg gagcgacaac cccctctctc
     2521 tttgaaaatc cccccagctc gagattctct tccaccgtca tctttggaaa gagttcacgt
     2581 ttttcaggta ccaaagcaag gccacgtttt accatctcaa aagcaggaag tccggtaatc
     2641 tcggtgccct caaagaagat ctgccctttc cgatcctttg tcaatccgaa gacgctctgg
     2701 actagagtag tcttccctgc gccattggct ccaaggagcg tcactatctc cccctgattt
     2761 ataaaaagag atacattcca caatacctga agaccagagt aaaagaccga gagctgcctg
     2821 atctcgagca gcggtgactc tgccctgttc atatatatcg ttcaccaaga tacgagtcga
     2881 ttacccgctg gtttttcacc acttcatccg ggatcccctc tgcgattttc tctccccggt
     2941 caaggacgac catcctgtcc gaaactccca ggatgacccg cataacatgt tcgatcatta
     3001 cgatcgtgat cccgtcgtca cggatggatt ttatgagaga aactgcttcc gtactttcgt
     3061 ttggattgag tccggtcatc atttcatcaa gaagtatcaa tttgggccgc gaagcaagcg
     3121 cccgggcaag ctgggttctc cgaagttcga ttacgttcag gcttgagagg ggcatgtcaa
     3181 tcctttcaag gggaaaacca atccggccga gtaattcttc tgcctctttt gcagctccac
     3241 tcatagtggc cttgtgcccg ttaccgaata gagatccgat catcacactc tcccttgcag
     3301 tgagaccggg aaaagagagg gtatgctgga atgttttcgc aatccccctc cggcagatct
     3361 tgtccattga gagtcttgaa atctcctctc cgagatactg aacagatccc ttatccggcc
     3421 ggtatatgcc ggaaattata ttgagaaggg ttgtttttcc cgcaccgttt ggtccgacaa
     3481 gcccgaggat ctcgccctca tggatatcga gatcaatgcc gttgactgca acaagacctc
     3541 caaatttttt ggttaccccc tccagcttga gcacttgtgt ttcacctctc tggtatccca
     3601 gctccaaact atgctacttg ttagcaagta acaataataa aagtatccat cgtacacgga
     3661 gagaaaagcg gattatggac cggttgaaaa ggatcagaag ggaatgtata tgatgtgtta
     3721 tgtcatgatg gtgatgatag agatcaatgg tttttccgga ctatcggcga gcctccatga
     3781 acaggatcat catctgtaga aaatgaagga ggtatatcaa caatgagaga attcaccagt
     3841 accctgaagg agaatgatct gtttccatgc cggaagagat tagggacctg atcgagaaga
     3901 ttaatcagga gggaataaag gctgccgaag agaaggctaa cattattgag gttgaggcaa
     3961 ggcagagagc agatgccatt ctctcccagg cacaggctga agctgacagg atgatcgctg
     4021 cagcagatca aagaatcatg cgagaagatg agagggagag ggccctcctt gcccaggctg
     4081 ggagggatct tcttctctcg ctccggcagg agataaatgt catgctcggg cggattgtgg
     4141 tctctgacat ccgcgaggct ctcacccctg agttactcta ccggctcctg tccgagataa
     4201 tcaggaatta tcccaaaatg gagcgaggca acattatcat ttcactcaaa aagaacgatt
     4261 tggatctcct ggaacaacag tatctccaga aactgcagct tgaggcagca aggggaataa
     4321 ccttgcagcc cggtgaagag atccagggcg ggtttactat cagcttcgac agtggaaaat
     4381 cctgctatga tttcaccgac aaagcactcg ccggatacat cataacctac ctcaaaccca
     4441 aattaaaaag gattcttgag ggatcaatag aggaatgaca taagatggga gagttttacc
     4501 cgtaccttat cgcaagcctg ccaacgctgc attttggaat gaaaccatca ttttcatttg
     4561 aacagttcct tgaaacatgc caccggctca tcccggaaaa agacttccag atcttaagta
     4621 acctgccacg acctgaaaat tactcagaag aaggcaggaa gcataagttc gttgaacgat
     4681 ggatcaagtt cgatactgcc ctccggaacg agacggtcag gatgcgggct ggcaggcgcc
     4741 atattgaggc tgcaacctat ctccgtcctg atacctaccc tggaccatct ctcgctcctg
     4801 tcgttgcagc agccactatg agcccttcat tgcttgaagg tgagaaggta ttggacgaga
     4861 cacgctggaa aatgctggat gaactcgcaa atgcccacta ttttgatctg gacgctctga
     4921 taacctatgc atataaactg ctgattctcc atcgttggga gaggatacga aatgccgatg
     4981 cagccctcct gctacgggaa gcactggagc aggaggcggt gtgaatgaca gcgttgtcat
     5041 cgggtcgaat cgtgcgaatt aatggtaaca tggttaccgt cacgtttgat actcccgtca
     5101 tgcaaaacga ggtagccttt atccggcaca gtgaggagcg tctcaaatct gaagtgatcc
     5161 gcatacgtgg taatctagct gagatgcagg tctatgaggc gacaacaggt ttaaagatcg
     5221 gggaggaggt tgagtttacc ggggaactcc tgtcgatcga gctcgggccg ggaatgcttg
     5281 gccaaatcta cgatggcctc cagaatcctc tgccggagat tgcggagcaa tgcggctttt
     5341 ttcttcagag aggggtttat ataaatgcac tttccgaatc gaagacctgg gattttactc
     5401 ccgaaaaaaa acccggtgat agagtaagag cgggagataa aattgggtat gttgaggaga
     5461 aaattttccg ccactactgc atggtccctt tcacattgac gggtaactat gagattatat
     5521 cgattgaaaa tgccggcagg ttcacgatac gccaggcggt cgcccaaata atggaccaga
     5581 acggaaaaat ataccctgtg tttttgagcc agacatggcc tgtgaagatc ccgatccaag
     5641 cttacacaga gagactgaag cctgaggaac ccctggttac caagatgcgt ctgattgatt
     5701 ccctcttccc ggtttcccgt ggagggacgt actgtatccc gggtcctttc ggagccggaa
     5761 aaactgtctt acagcagttg atcagccgtc acgccgaggt cgatatcgtg attatcgccg
     5821 cctgcggtga acgggcgggt gagattgttg agacattacg gacgtttcct tctattatcg
     5881 atccgagaac cagccgtcct ttgagcgatc gcacggtcat tatctgtaac acgagctcaa
     5941 tgccggttgc agcgagggaa tcgagcgttt atacggcggt cactatcgct gaatactatc
     6001 gccagatggg actccacgtg cttctgcttg cagattcaac ttcccgatgg gcccaggcca
     6061 tgcgtgaaat gtcagggaga ctggaagaga tccctggcga ggaggcattc cctgcatatc
     6121 tggagtcgcg aatcgcctcg ttctatgaga gggcaggagt ggttcgttta cacgacggct
     6181 ctttaggctc ggtgacaatc gggggggctg tcagccctgc gggggggaac tttgaagaac
     6241 cggtgaccca ggcaacatta aaagtagtag gtgctttcct tggcctctcc cgcagtcgtt
     6301 ccgatgccag acgttatccc gccatagatc cactcatcag ctggagcaaa tacaggagta
     6361 tcatcgatga gagacagaga gagagggggc aggggatcct gctcaaaggc aacgatatcg
     6421 cccagatgat gaaagttgtc ggtgaagaag gaacatcact ccaggattac atggattttc
     6481 tcaaagctga atttttcgat tttgtctatc tgcagcagaa tgccttcgat ccggttgatg
     6541 aagccaccat gaaagatcgg cagatctata tatttgattt tattcaccgc attattgaat
     6601 cagaatttgt ttttgaacgg aaagatgaag cactccattt tttccagcag ttgagacagc
     6661 tcttccgagg atggaactca atctcctggc agagtaatga atttaaaaag accgagaaag
     6721 agatcagggc atttctcctc gttcgcacaa gggaggggaa gcatgcataa gatctatacg
     6781 aacataaacc agattgtcgg cgatgttatt gccgtggatg cagaaaatgt cggcaatacg
     6841 gagcttgccg aggtgacaac ccggcgggga acgtcccttg cccaggttat tcgccttgac
     6901 gggaacaaag cattcttaca ggtctttgca ggcagcagag gaatttcaac gggagaccgg
     6961 gtaaggtttc tcgggcatcc gatgcgtatt gctaccggtg acctgctcct tggccgggtg
     7021 tttaacggaa gcggtcgccc cctcgataat ggtcctgatc tttccgagaa cctagtcgat
     7081 atcggtgggc cgccggtcaa tcccgtgaaa cggatcattc caaacaagat gattcggacc
//
LOCUS       DAOOCO010000094         7123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9862_length_7123_cov_5.341539, whole genome shotgun sequence.
ACCESSION   DAOOCO010000094 DAOOCO010000000
VERSION     DAOOCO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_7123_cov_5.341539"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..2230)
                     /gene="uvrB"
                     /locus_tag="PK154_06350"
     CDS             complement(299..2230)
                     /gene="uvrB"
                     /locus_tag="PK154_06350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPW10718.1"
                     /translation="MTGFDLKARFGPAGSQPEAIRELVSGLGQNERFQTLLGVTGSGK
                     TFTMANVISEAERPALVIAHNKTLAAQLWNEFREFFPNNRVEYFVSYYDYYQPESYLP
                     AKDQYIEKDAQINPKIEMMRLSATASILSRRDVIVVASVSCIYGLGRPEHYRNMGFEL
                     KVGDTISRREIIGHLVASLYERNEIELMPGRFRVKGDTIDLIPGYFNNIIRIELFGDQ
                     VDRIAEIDRNSGETREKMNYFYVFPARHYVIPQELQKQALRSIQQELEERLPELGMIE
                     AHRLTQRTMYDIEMIEETGSCKGIENYSRHFDFRKPGEKPYCLLDYFPPDFLLFIDES
                     HQTIPQLNGMYRGDRSRKQALVDYGFRLPSAFDNRPLMFREFEHYMKNVIFVSATPGP
                     YELAHSGQIVEQIIRPTGLVDPIVEVRTTVGQTENVMGEIQETIARGDRVLLTTLTKR
                     LAEELTEYLAGRGIRTRYLHSEIDTLQRTEIIRELRLGRFDVLVGINLLREGLDIPEV
                     GFIGILDADKEGFLRDARSLIQIIGRAARNVNSRVVLYADSLTDSIKAAVSETERRRT
                     LQMEYNRIHQISPVTIKKPVPEKEVDLKDTRHIPKSDIPNLIIELEARMNKAAENLEF
                     EEAIRLRDRIRDLREREGR"
     gene            complement(2227..3777)
                     /gene="uvrC"
                     /locus_tag="PK154_06355"
     CDS             complement(2227..3777)
                     /gene="uvrC"
                     /locus_tag="PK154_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPW10719.1"
                     /translation="MIEIATLPHAPGCYLFRGSGGTILYIGKAKDLKKRVSSYFRKRD
                     LDPKTRLMVEAAEDLDYIVTKNEVEALILENSLIKKHQPRYNIDLKDAKAYAYLQLTD
                     DPFPAIRIARNPSGRGTFFGPFVSAAEREYIHDLLKRTFRLRTCKKLQKRGCLRYHIH
                     TCSAPCRGEISSDEYADLVRKATLVLKGRTGELLATLKQEMAARSQKEDFEGALVIRD
                     QSQALEPLSYRQDITRRKEGDEDIISYRAYQGTVHLMFFNVYRGTLGNKSEFSFEEHP
                     DFFEEFLVQYYSDNPVPSEIILPEPISEPVAEFLTLARGKRMTVTVPKIGAKRRLLDL
                     VEKNIETVFYAGEMRVMELQEALGLDSPPEVIECFDISHLSGSSVVGSMVRFRNGKPE
                     KKEYRRFRIRTVEGIDDSKAIGEVISRRYSRLLKEGALLPDLVMVDGGRTQLSAALAA
                     LKEIGAGIPVIALAKREEQVYVPGRTTPLPLSRKEQGSLYLQEIRDEAHRFAIAYHHL
                     LRTKELVR"
     gene            complement(3774..6575)
                     /gene="uvrA"
                     /locus_tag="PK154_06360"
     CDS             complement(3774..6575)
                     /gene="uvrA"
                     /locus_tag="PK154_06360"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW10720.1"
                     /translation="MKKIVIRGARQHNLKNITVELPRDQFIVITGLSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMSKPDVDAIEGLSPAISIEQKSTSKNPRSTVGTVT
                     EIYDYLRLLFARIGTAYCPVHNIRIESQSPERIADTIREKMTGTVTILSPVVRQKKGT
                     YQQLIKDLNREGYLRVRINGEIQRTDEEFTLERYRKHDIEIVIDRLDPSDRSRLAEAC
                     EAALARSGGLLIVMDEAGDDTLFSATMACPVCGVTFEELQPRMFSFNSPFGACEECNG
                     LGIKMEFDPDLIIPDRSLSIADGAVALYRNFLDGYRSQYLAAVARHYGFSIFTPIVEI
                     SEEQYNALMFGSDEKIKFSMSMKNGEGHWAHTGSWEGLLPQAQRLYHQTQSEYRKREL
                     ERFMRISPCQRCGGKRLKEKVLAVRVQDKSIIDITDMPVGDCIQFFRDLALTEKEAEI
                     ARQILKEINARLDFLEKVGLGYLTLSRNAGTLSGGEAQRIRLATQIGSNLMGVLYVLD
                     EPSIGLHQKDNQKLIDTLRRLRDLGNTLVVVEHDEDTIRSADYVVDMGPGAGLHGGYV
                     VAEGTPGEIEGSSESLTGQYLAGKLKINVPKKRRKNSRYLRISGCQENNLKNIDVRIP
                     LGVFTVITGVSGSGKSTLIYDTLYQALQKKLYKSRVTPGKYRKIEFDSEVDKVIVIDQ
                     SPIGKTPRSNPATYTKVFDEIRQAFAGTKEAKVRGYKPGRFSFNLKGGRCEACEGDGL
                     IKIEMNFLPDVYVECEECRGRRYNRETLEVKYKGKSIAEVLDMTVEEALTHFEHLPVI
                     RNKLETLSAVGLDYIKLGQSSTTLSGGEAQRIKLTRELARKATGKTIYLLDEPTTGLH
                     FHDVKKLIAVLSELVEKGNTVVVIEHNLDVIKSADHIIDLGPEGGDAGGYIVATGTPE
                     EVAANPESHTGRFLQKMFA"
     gene            6749..>7123
                     /locus_tag="PK154_06365"
     CDS             6749..>7123
                     /locus_tag="PK154_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPW10721.1"
                     /translation="MQKVYVIGHRQPDTDSIGSVIGYTDLLNSREPGRFIASYSGDLN
                     AETRFALGYFGLTAPFEISSVEPNVGDIPFFYTQKAPADMPTIDVAAMMDEYEIRNIP
                     IIDNEGKFLGLVSEHGLAKAYVT"
BASE COUNT         1301 a         2091 c         2043 g         1688 t
ORIGIN      
        1 aaaatctcgg ggggatccct cagctttttt ttataaaata acatgattcc tttgagggcc
       61 accagcatta aagatgaaaa tgccagaggc attttcatat aaaaaaaagt gaaccagcaa
      121 cgagcgagcg tcctggccat atttgtgcca gctgtgttct tcgggacgga aacgatccct
      181 ccctagttgt caccgatcag atcgtttttt ttattgaaaa gccaggagca gttgctggat
      241 ctgaccggat gtctcccggc agaattctca cgcaccctcc catccttttt cggtacacct
      301 accttccttc ccgctcccga aggtcgcgga tccggtcgcg gagccgtatc gcctcttcaa
      361 actcgaggtt ctctgcggcc ttgttcatcc gggcttcgag ctcgatgatc aggttcggga
      421 tatcgctctt cgggatatgc cgggtgtcct tcaggtccac ttccttttca gggaccggtt
      481 ttttaatggt cactgggctg atctgatgga tccggttata ttccatctgg agtgttcgtc
      541 tccgttcggt ctcgctcacc gctgccttga tcgagtctgt cagggaatcg gcgtatagca
      601 cgacccggga gttgacgttc ctcgctgccc tcccgatgat ctggatgagg cttcgggcat
      661 cccggaggaa tccttccttg tcggcgtcaa ggatcccgat gaagccgacc tcaggaatgt
      721 caagcccttc ccgcaggagg ttgatcccga caagcacgtc aaaccttccg agacggagtt
      781 ccctgatgat ctcggtccgc tggagggtgt caatctcgga gtggaggtac cgcgtccgga
      841 ttcccctgcc ggcaaggtac tcggtcagtt cctcagccag ccgcttggtg agggtcgtga
      901 ggaggacccg gtcacccctg gcaatggtct cctggatctc ccccatcacg ttctcggtct
      961 gtcccacggt ggtcctcact tccactatcg ggtctacgag gccggttgga cggatgatct
     1021 gctcaacgat ctgccccgag tgggcaagct cgtagggacc gggggttgcc gagacgaaga
     1081 tcacgttctt catgtagtgt tcaaattccc ggaacatcag gggacggttg tcaaacgcgc
     1141 tcggcagccg gaacccataa tcgacgagcg cctgcttccg tgagcggtcc ccccggtaca
     1201 ttccgttgag ctggggaatg gtctggtggc tctcatcgat gaagaggagg aagtcgggag
     1261 ggaagtaatc cagcaggcag tagggcttct cgcctggttt ccggaaatca aaatggcggg
     1321 agtagttctc gatccccttg cagctccctg tctcctcgat catctcgatg tcgtacatgg
     1381 tccgctgggt gagccggtgg gcctcaatca tcccgagttc cgggaggcgc tcctccagct
     1441 cctgctggat cgaccggagt gcctgcttct ggagctcctg ggggataacg tagtgtcggg
     1501 cagggaagac atagaagtag ttcatcttct ctcttgtctc ccctgaattc ctgtctatct
     1561 cggcgatccg atcgacctgg tcaccgaaga gctcgatccg gatgatgttg ttgaaatacc
     1621 cggggatcag gtcgatcgtg tcccctttca cccggaacct gccgggcatc agctcgatct
     1681 cgttccgttc gtagaggctg gcgacgaggt gcccgatgat ctctctcctt gagatggtgt
     1741 cccccacctt cagttcgaac cccatgttcc ggtagtgctc gggacggccg agcccataga
     1801 tgcaggagac tgaggcgacc acaatcacgt ccctcctcga gaggatcgat gccgttgcgg
     1861 agaggcgcat catctcgatc ttcgggttga tctgggcatc cttctcaatg tactggtctt
     1921 ttgccggcag gtaggactcc ggctggtaat agtcatagta cgagacgaag tattccaccc
     1981 ggttgttcgg gaagaattcc ctgaactcgt tccagagctg ggctgccagg gtcttgttgt
     2041 gtgcgatcac cagtgccggc cgctcagcct cgctgatcac gttcgccatg gtgaaggtct
     2101 tgccggaacc agtcacgcca aggagggtct ggaaccgttc gttttgtccg aggccggaga
     2161 caagttccct gatcgcttcc ggctgtgagc ctgccggccc gaagcgtgct ttgagatcga
     2221 acccggtcat cggacgagct cctttgtcct gaggagatgg tggtacgcga tcgcgaaccg
     2281 gtgggcctcg tccctgattt cctggaggta cagggacccc tgctcttttc gtgacaatgg
     2341 cagaggggtg gtcctgcccg ggacatatac ctgctcctcc ctctttgcaa gcgcgatgac
     2401 cgggataccg gcaccgatct ccttcagtgc tgccagtgcc gcggagagct gtgtccttcc
     2461 gccgtcaacc atgacaaggt cgggcaatag tgctccttct ttcaggagcc gtgaatatct
     2521 cctgctgatc acctcgccga tggctttcga atcatcgatc ccctccaccg tccttatcct
     2581 gaaccgccgg tactccttct tctccggttt tccattcctg aaccggacca tggatcccac
     2641 caccgaggat cctgagaggt gggagatgtc aaagcattcg atcacctccg gcggagaatc
     2701 cagcccgagg gcctcctgga gctccatcac gcgcatctcc ccggcataga acacggtctc
     2761 gatgttcttt tcgacaaggt cgaggagccg tctctttgcc ccgatcttcg gcaccgtcac
     2821 ggtcatccgc tttccccggg caagggtgag gaattctgcg accggttcgc tgatcggttc
     2881 aggcaggatg atctcggacg gtaccgggtt gtcggagtag tactggacaa ggaactcttc
     2941 aaaaaaatcc gggtgctcct caaaggagaa ctcgctctta ttccccaggg tcccccggta
     3001 gacattgaag aacatcaggt gcacggttcc ctggtaagcc cgatagctga tgatatcctc
     3061 gtccccctct ttcctcctgg tgatgtcctg ccggtatgag aggggctcga gcgcctggct
     3121 ctggtcccgg atgacgagcg ctccttcgaa atcctccttc tgcgatcgcg ctgccatctc
     3181 ctgtttcagg gtcgccagaa gctcccctgt tcttcccttg agcactaagg ttgctttcct
     3241 cactagatca gcgtactcat cgctgctgat ctcccccctg cagggggcac tgcaggtgtg
     3301 gatatggtag cggaggcagc cccgtttctg gagttttttg caggtccgca gcctgaaggt
     3361 ccgtttcagc aggtcatgga tatactcgcg ctcggctgcc gagacgaagg ggccaaagaa
     3421 cgtccccctc cctgaagggt tcctggcgat cctgatggcc ggaaaagggt catcggtgag
     3481 ctggaggtag gcataggcct tcgcgtcctt caggtcgatg ttataccggg gctggtgctt
     3541 cttgatcagg ctgttttcaa ggatcagcgc ctcgacctcg tttttggtca cgatatagtc
     3601 gagatcctcc gcggcctcga ccatcagcct ggtctttgga tcgaggtcac gcttccggaa
     3661 gtagctgctg accctttttt tcaggtcctt tgctttcccg atatagagga tcgtgccacc
     3721 actgcccctg aacaggtagc atcccggtgc atgggggagg gtggcgatct cgatcatgca
     3781 aacatcttct ggaggaaccg gccggtatgg ctctccgggt ttgccgcaac ctcctcgggt
     3841 gtcccggtcg cgacgatgta ccccccggca tcaccccctt cagggccgag gtcgatgata
     3901 tgatcggctg acttgatcac atcaaggttg tgctcgatca ccaccacggt gtttcccttc
     3961 tccacaagtt cgctcagcac cgcgatcagc ttcttgacat catggaaatg gagccccgtg
     4021 gtcggttcat cgaggagata gatggtcttt cccgttgctt tcctggcaag ctcccgggtg
     4081 agcttgatcc gctgtgcttc ccctcccgag agcgtggtcg agctctgccc gagcttgatg
     4141 taatcgaggc cgaccgcgga gagagtctcg agcttgttcc ggatgaccgg gaggtgttcg
     4201 aagtgggtga gcgcctcctc gacggtcatg tcaagaacct cggcgataga cttccccttg
     4261 tacttcacct cgagcgtctc gcggttatac cgcctgcccc ggcactcttc gcattcgacg
     4321 tagacgtcag ggaggaagtt catctcgatc ttgatcagcc cgtccccctc gcaggcctcg
     4381 cagcggcctc ccttcaggtt gaacgagaac cttcccggct tatagccacg gaccttggct
     4441 tccttggtcc cggcgaacgc ctgacggatc tcgtcaaaga ccttggtgta ggtggcaggg
     4501 ttgctcctgg gtgtctttcc gatcggactc tggtcgatca cgatcacctt gtcgacctcg
     4561 gagtcgaact cgatcttccg gtacttccct ggagtgaccc gggacttgta cagctttttc
     4621 tggagcgcct ggtacagggt atcatagatc agcgtcgatt tgccgctccc ggacaccccg
     4681 gtgatcaccg tgaagacgcc gagcgggatc ctgacatcga tgtttttaag gttgttctcc
     4741 tggcagccgg aaattctgag gtaccgggag ttcttccgtc gtttcttcgg gacgtttatc
     4801 ttcagttttc ccgcgaggta ctgcccggtg agggattcgc tgctcccttc aatctccccg
     4861 ggggttcctt ccgcaaccac gtacccgcca tgcaggccgg cgcccggccc catgtccacg
     4921 acataatcgg cgctccttat cgtatcctca tcatgctcga ccacgacgag cgtgttcccg
     4981 aggtcacgga gcctccggag tgtatcgatc agcttctgat tatccttctg gtggaggcca
     5041 atggagggtt catcaagcac atacagcacc cccatcaggt tggaccctat ctgggttgcc
     5101 aggcggatcc gctgcgcctc gccgccagaa agggtgccgg catttcgtga gagggtgagg
     5161 tatccgagcc cgaccttctc gaggaagtcc agccgtgcat tgatctcttt caggatctgc
     5221 cgtgcgatct ctgcctcttt ctcggtcagg gcgaggtcgc ggaagaactg gatgcagtcc
     5281 ccgacgggca tatcggtgat atcgatgatg gacttgtcct gcaccctgac ggcgaggacc
     5341 ttctccttga gcctctttcc cccgcagcgc tggcagggtg atatccgcat gaagcgttcg
     5401 agctcgcgtt tccggtactc ggactgggtc tggtggtaga ggcgctgcgc ctgggggagg
     5461 agtccctccc acgatccggt atgagcccag tgcccctcac cgttcttcat gctcatggag
     5521 aacttgatct tctcgtcaga gccgaacatc agggcattgt actgctcttc actgatctcc
     5581 acgatcgggg tgaagatgga gaacccgtag tggcgggcta ccgcggcaag atactggctc
     5641 cggtagccat caaggaagtt ccggtacagg gcgaccgctc cgtcagcaat cgagagggac
     5701 cggtccggga tgatcaggtc ggggtcaaat tccatcttta tcccaagccc gttgcactcc
     5761 tcgcacgccc cgaaggggct gttgaacgag aacatccgtg gctggagttc ctcgaaggtg
     5821 accccgcaga ccgggcacgc catggtagcc gagaacaggg tgtcgtcccc tgcctcatcc
     5881 atgacaatca atagcccgcc cgacctggcg agggcagcct cgcacgcctc cgcaagccgg
     5941 gaacggtctg aaggatccaa gcggtcgata acgatctcga tgtcatgctt ccggtagcgt
     6001 tcgagtgtga actcctcatc ggtgcgctgt atctcgccgt tgatccgcac ccggaggtat
     6061 ccttcgcggt tcaggtcttt tatcagctgc tggtaggtgc cctttttctg ccggaccacc
     6121 ggggaaagga tggtaacggt cccggtcatc ttttccctaa tggtatccgc gatccgttca
     6181 ggggactgcg actcgatccg gatgttgtgc accgggcagt atgcggtccc gatccgggcg
     6241 aagaggagtc gtaagtaatc gtagatctcg gtgaccgtgc cgaccgtgct cctcgggttc
     6301 ttgctggtgc tcttctgctc gatggagatg gccggggaga gcccctcgat ggcatccacg
     6361 tccggtttgc tcatcagtcc gaggaactgc cgggcgtatg ccgagagcga ttccacgtat
     6421 cgccgctggc cttcggcata gatggtatcg aacgcgagcg ttgacttgcc tgaaccggag
     6481 aggccggtga tgacgatgaa ctggtcccgc ggtagctcga cagtgatatt cttgaggtta
     6541 tgttgccgag ccccgcggat aacgatcttt ttcattcttt ctgcgggatc aaatctctcc
     6601 tccaagcata tagatatcct catcacggta atctccggtg acaggttttt accgtgattc
     6661 gctatgcggt attcccgggc tgatccgaag gtgagggtta aacctgtgta cttcacagta
     6721 tagtgggata agcgcgaagg gagagattgt gcagaaggtg tacgtgatcg ggcaccgcca
     6781 gcctgatact gacagcatcg ggagcgtgat cgggtatacc gatctcctca actcccggga
     6841 accgggacgg ttcatcgcct cgtactcggg ggacctgaat gccgagacca ggtttgccct
     6901 gggatacttc gggctcactg caccgttcga gatatccagc gtcgagccaa atgtcggcga
     6961 tattccgttc ttctataccc agaaggctcc tgcggacatg cccaccatcg atgtcgcggc
     7021 catgatggac gagtacgaga tcaggaacat cccgatcatt gacaacgaag ggaagttcct
     7081 cgggctcgtc agcgagcacg ggctagcaaa ggcatatgtg act
//
LOCUS       DAOOCO010000095         7121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9869_length_7121_cov_5.752052, whole genome shotgun sequence.
ACCESSION   DAOOCO010000095 DAOOCO010000000
VERSION     DAOOCO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9869_length_7121_cov_5.752052"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PK154_06370"
     CDS             <1..1273
                     /locus_tag="PK154_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW10722.1"
                     /translation="EKITGVPTETVKEMARKYASAKNGVIIYCLGITELTTGTDNVRS
                     LGNLSMLCGNIGRPGVGVNPLRGQNNVQGACDMGAYPNVYSGYQKCELPEMRKKMEDA
                     WGMPAGSLPEWYGSTLTEQITQCGDPIKAMYIFALNPAVSYPDSNHVKRSLDKLDFLV
                     VQDIFFTETCEYADVILPGASFAEKDGTFTSGERRINRVRKAVNPPGEAKEDWEIFVL
                     LAKKLGLKGFDFKSPQEIWDDMRSVTPSMFGATNPRIDKPGSVHWPCPTEEHPGTPIL
                     HKEKFASPDGLGTFFGIEYRPPAEVADAEYPFTLMTGRLIFHYHTRTQTDRAKVLHYE
                     VPENFVQINTEDAKRLGIKEGEHIKVRSRRGETTPLARVTDEVAPGVLYMAMHFPNGA
                     NNLTNTALDPLSKMPELKHCAVAVEKISEAK"
     gene            1276..2511
                     /locus_tag="PK154_06375"
     CDS             1276..2511
                     /locus_tag="PK154_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPW10723.1"
                     /translation="MSKKGDMYYAWTSDADLAKKAECGGAVSGLLKYALEKKIVDGVF
                     AVTRGQDIYDAVPVFITDPKDIDKTAGSLHCGTLLMSKLVKKYLDGAGTQKIAMTVKG
                     CDMMGLYELAKRQQINLDNIIMIGVNCGGTVSPVTARKMITEKFGVDPDTVHKEEIDK
                     GQFIIEYEGGHKGISIDELEEEGYGRRSNCRRCKMKVTRQADLACGNWGVIGEKAGKA
                     TFVEVCSAKGADLLDRAVKDGVIKTEAPNPKGLEIRAKVENAMYKLSDKWRKKDFEGL
                     GEGRERLKKIVKETSRCIKCYACIENCPICYCVECSTKKPYLVKPGEVPPNFMFHLIR
                     FAHIADSCVNCGQCQELCAMDIPNALFMHAQQVELEKMFGHVPGVDMSLPLLALVEER
                     EERDRLSATGSDQIFDIFK"
     gene            complement(2640..3233)
                     /locus_tag="PK154_06380"
     CDS             complement(2640..3233)
                     /locus_tag="PK154_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPW10724.1"
                     /translation="MDAYGIGPALQEQFTRCISFHTVTAPGLFIGVFMVDYALELLGA
                     RHGEKLYAIAETYKCIPDPVQVMAGCTFGNHRLQVVPIGKFALTMNRPSEREAIEGIR
                     VFVDAEKLRKYRIIHAWYTHSPGFDSRTMIYPLIDEILKAGRDILSYQRVRVNVPLKR
                     SWKSVRCTTCGEMVPDIALDGDQCKGCGSLSYYDVVQ"
     gene            3482..5554
                     /gene="fdhF"
                     /locus_tag="PK154_06385"
     CDS             3482..5554
                     /gene="fdhF"
                     /locus_tag="PK154_06385"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPW10725.1"
                     /translation="MSTMSDKALKYVPTTCPYCGVGCGLNLVVSDGKLVGVEPYKRSP
                     INEGKLCPKGMTCWEHVQSPDRLTKPKIKKNGKFVDASWDEAIDLIVKKFTEIYKQHG
                     PKALGFQTSCRTVNEDCYALQKFARCAFLTNNVDNCARICHGPSVAGLSLSFGSGAAT
                     NPFEDVLNSDLLLIWGSNAVEAHPLAGRRIMQAKQKGIPIVVVDPRYSATARLADKWV
                     RFNPSTHIALANNMMYYIIKEGLEDKAFIAERTKGFDDLKAMVQKYADATNIHGVPQE
                     TVKEIARQYATAKNAVIIYCLGITELTTGTDNVRSMGNLSLVTGNIGRPGVGVNPLRG
                     QNNVQGACDMGAYPNVFAGYQAVAVPENRAKHEKAWFLKEGSLPDWYGSTLTEQINDA
                     GTVVKAMYFMGLNPTVSYPDSNHVRQQLEKLDFCVMQDIFWNESCDYADVVLPGTCFA
                     EKDGTFTSGERRVNRVRKAVDGPGESKYDWEIISMLAKKMGLKGFDWKSAKDVWDDMR
                     AVTPGQFGVTYEKMEKPESVHWPCPTIEHPGTPILHIGKFSAADGKGTMFGLEYRPPA
                     EVADAQYPFTLMTGRVIFHYHTRTQTDRAAQLHYEVPESYIQINTEDAKKLGIKEREK
                     IKVSSRRGEIETLARVTDDVAPGVLYMAMHFHKGANTLTNTALDPLSKMPELKHCAVK
                     VEKIAEAK"
     gene            5555..6730
                     /locus_tag="PK154_06390"
     CDS             5555..6730
                     /locus_tag="PK154_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPW10726.1"
                     /translation="MALFGGKAEAKKGDMLYAWTNDADLAKKAECGGAVTELLKYALE
                     SKTVDAVLAVKRGVDLYDAVPTIVTNPKDLDACAGSLHCGTLLLSKLVMEYVPKLSGK
                     KLGMVVKGCDLMGLLELSKRKLVNLDNIVMIGVNCGGSVSPVTARKMIKEKFGVDPNT
                     VTKEEIDKGQFIIEYEGGHKGISMDELEEAGYGRRSNCRRCKLKVPRQADLACGNWGV
                     IGDKAGKATFVEVCSDKGAKLLNAAVKANKLTAEPANQKGIELRTKVENSMYKLGDKW
                     RKHDFEALAPNLWETIAKETGRCMKCYACIDHCPVCVTIADKWKQPDQMVRAGFIPAD
                     PMFHLRRFAHISDSCINCGQCEEGCPSEIPLALFSHAIRTEADKFFEPKLGKPAYTN"
     gene            6781..>7121
                     /locus_tag="PK154_06395"
     CDS             6781..>7121
                     /locus_tag="PK154_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW10727.1"
                     /translation="MKADDAKKIIKDAIDKEIEAYTFYRGIADKVKDKALKDLFAELA
                     GEEKKHREFLQGVLDKDVSKMKFDPSHDYKVGDNLPTPELKVEMKPLEGLVLAIKKEL
                     QAMQMYTQLAKL"
BASE COUNT         1820 a         1858 c         1977 g         1466 t
ORIGIN      
        1 tgagaagatt accggggtac ctaccgagac cgtaaaggag atggcacgga agtacgcctc
       61 tgcaaagaat ggcgtcatta tctactgcct tggcatcacc gagctcacta ccgggactga
      121 caacgtccgg tcgctcggga acctctcgat gctctgcggg aatatcgggc gccccggtgt
      181 cggtgtcaat ccgctccgtg gccagaacaa cgtgcagggc gcctgtgata tgggcgctta
      241 tccaaacgtt tattcgggat accagaagtg cgagctcccg gagatgcgga agaagatgga
      301 ggatgcctgg ggcatgccgg ccggatcgct gccggaatgg tacgggtcga cattgaccga
      361 gcagatcacc cagtgcggcg atccaattaa agcgatgtat atatttgctt taaacccggc
      421 cgtatcatac ccggactcga accatgtgaa gcgttcactc gataaactcg actttttggt
      481 agtccaggat atcttcttca ccgagacctg cgagtatgcc gatgttattc tccccggggc
      541 atcctttgca gagaaagatg ggacgttcac cagcggggag cggcggatca acagggtgcg
      601 gaaagccgtt aatcccccag gtgaggcaaa ggaggactgg gagatctttg tccttcttgc
      661 aaagaaactc gggctgaaag gattcgattt caagtcaccg caggagatct gggatgacat
      721 gcgatcagtc accccgtcaa tgttcggtgc caccaatcca aggatcgaca agccaggatc
      781 cgtccactgg ccttgcccga cggaggagca cccgggaaca cctatcctcc acaaggagaa
      841 gttcgcttca cctgacggcc ttggcacctt cttcggaatc gagtaccgcc cacctgccga
      901 ggtagcagat gcggaatacc cgttcaccct gatgaccgga cgtctgatct tccattacca
      961 cacacggacc cagaccgacc gggcaaaggt gcttcattac gaagtaccag agaacttcgt
     1021 ccagatcaat actgaggatg cgaagagact ggggataaaa gagggtgaac atatcaaagt
     1081 gcgcagccgc aggggagaga ccacgccact tgcccgtgta accgacgagg ttgcccccgg
     1141 tgtcctctac atggccatgc acttcccgaa cggcgcgaac aacctcacca ataccgcact
     1201 cgacccgctc tccaagatgc ccgaactgaa gcactgtgcc gttgccgttg aaaagatctc
     1261 ggaggcgaag tgagcatgtc caagaaaggc gacatgtatt atgcatggac ctccgatgct
     1321 gacctcgcaa agaaagcaga gtgcgggggg gccgtgagcg gcctcctgaa atatgcgctc
     1381 gagaagaaga ttgtcgatgg ggtttttgcg gtcacccggg gtcaggatat ctacgatgcg
     1441 gtcccggtat tcatcaccga tcccaaggat attgataaaa ctgccggatc gctccattgc
     1501 gggactctcc tcatgtccaa gctcgtcaag aaataccttg acggggctgg cacccagaag
     1561 atcgcgatga cggtgaaggg ctgtgacatg atggggctct acgagcttgc caagcggcag
     1621 cagatcaatc ttgacaacat catcatgatc ggtgtgaact gcggcggcac ggtctccccg
     1681 gtcaccgcac gaaagatgat cacagaaaag ttcggagtag atccagacac cgtccacaag
     1741 gaggagatcg acaaaggtca gttcatcatc gagtatgagg gaggccacaa gggtatctcc
     1801 atcgacgaac tcgaggagga gggttatgga cggaggagca actgccgcag gtgcaagatg
     1861 aaggtgaccc gccaggcaga cctcgcctgt ggaaactggg gggttatcgg ggaaaaggct
     1921 gggaaggcaa cgttcgtgga ggtctgcagc gccaagggag ccgacctcct tgacagggct
     1981 gtcaaggatg gcgtgatcaa gaccgaggcc ccgaacccca agggtcttga gattcgggcc
     2041 aaagtcgaga atgcaatgta caagctcagc gacaagtggc ggaagaagga ctttgagggc
     2101 ctgggtgagg gaagggagcg tcttaaaaag atcgtaaagg aaacctcgcg gtgcatcaag
     2161 tgctatgcct gcattgagaa ctgcccgatc tgctactgcg tcgaatgcag cacgaagaaa
     2221 ccatatcttg tgaagcctgg agaggtccca cccaatttca tgttccacct catcaggttc
     2281 gctcatattg ccgattcctg cgtgaactgc ggacagtgcc aggaactctg tgcgatggat
     2341 attcccaatg cattgttcat gcatgcccag caggttgaac ttgagaagat gttcgggcat
     2401 gttcctggtg ttgatatgtc acttccactc cttgcgcttg tcgaggagcg tgaggagcgt
     2461 gaccggctct cagcgaccgg aagtgaccag atctttgata tattcaaata accccttttt
     2521 ccggaaattg tcagatattc ccaaaaaacg cctttgtgct gatcgtaccg gtgtggcgga
     2581 aactatgact tatcaacggt ttcgcagtga acaagggata atactgcctt gttcacggtt
     2641 tattgaacaa catcgtaata ggatagtgaa ccgcaccctt tgcactgatc tccatcgagc
     2701 gcgatatccg ggaccatctc tccgcaggtt gtgcacctga ctgatttcca tgaacgcttg
     2761 agaggcacat tgaccctgac acgttgatat gagagaatat cacgtccagc tttcaggatc
     2821 tcatcgataa gagggtagat cattgtccgg ctgtcaaatc cgggactatg ggtataccag
     2881 gcatgaataa tccggtattt tcttaacttt tcagcatcga caaagaccct gattccctcg
     2941 attgcttccc tttctgaagg gcggttcatt gtcagagcaa acttgccaat tggtacaacc
     3001 tggaggcggt gatttccgaa tgtgcatccg gccatgacct ggacgggatc ggggatgcat
     3061 ttgtacgttt ctgcaatagc atagagcttc tcaccatgac gggcaccgag aagttccagc
     3121 gcatagtcca ccatgaagac gccgatgaag agtcccggtg cagtcaccgt gtggaaactg
     3181 atacaccggg taaactgttc ctgaagggca ggaccgatcc cataggcatc catactctcc
     3241 cgcatttcag tggaagcgcg gggctgggtc gtaaattgca taaaaagcga agggtccctg
     3301 cttttataag gattgtgtag agacttccct ttcgatactt cataaaatgt taatatatat
     3361 atattaacaa ataaactaaa tttattaata tttaaataac atgaagtttg agtattgaac
     3421 tataaacact agttaatcag aagtccggat tttacgccgg atatctccta aagaggggga
     3481 tatgagtacc atgagtgata aagctttgaa gtatgtaccc acaacctgcc cgtattgcgg
     3541 agtcggctgt ggactcaacc ttgttgtcag cgatggaaag ctggtagggg tagaacccta
     3601 caagcggtcc ccgatcaacg aaggcaagct ctgtcctaag ggaatgacct gctgggagca
     3661 tgtccagagc cccgaccgcc ttacgaaacc gaagatcaag aagaacggca agttcgtgga
     3721 cgcttcgtgg gatgaggcca ttgacctgat cgtcaaaaag ttcacggaga tctacaaaca
     3781 gcacgggcct aaggcgcttg gcttccagac atcgtgccga accgtcaacg aagactgcta
     3841 tgctctccaa aagtttgccc gttgtgcatt cctgaccaac aacgttgata actgcgcccg
     3901 tatctgccac ggcccttcag tcgcagggct ctcgctctcg tttggatcgg gtgcagcaac
     3961 caacccattc gaggatgtcc tgaactctga cctccttctc atctggggtt ccaatgcagt
     4021 cgaggcccac ccgctcgctg gccggcgcat catgcaggca aagcagaagg gcatcccgat
     4081 tgtcgtggtt gacccacggt atagtgcaac cgcacggctt gccgacaagt gggtccggtt
     4141 caacccgtcg acccacattg ccctcgccaa caacatgatg tactacatca tcaaggaagg
     4201 gctcgaggac aaggcgttca ttgcggaacg gacgaaaggc ttcgatgacc tgaaagcgat
     4261 ggtccagaag tatgctgatg ccacgaacat ccacggtgtc ccgcaagaga cggtgaagga
     4321 gattgcccgc cagtatgcga ctgcaaagaa tgcggtcatt atctactgcc ttgggatcac
     4381 cgagttaacc accggtactg ataacgtccg ttcaatgggc aacctatcgc tggtcaccgg
     4441 caacatcggc cgtcccggcg tcggtgtcaa cccgctccgc ggccagaaca acgtgcaggg
     4501 tgcctgcgac atgggtgcat acccgaatgt ctttgccggg taccaggcag tggctgttcc
     4561 cgagaaccgt gccaaacacg agaaggcctg gttcctcaag gaaggttcac tccccgactg
     4621 gtatggatcg accctcaccg agcagatcaa tgatgccggc actgttgtca aggcaatgta
     4681 tttcatgggc ctgaacccga cggtatccta ccccgactcg aaccatgtca ggcagcagct
     4741 cgagaaactc gacttctgcg tcatgcagga tatcttctgg aacgagagct gtgactatgc
     4801 agatgttgtc cttcccggca cctgctttgc cgaaaaggac ggtacgttca cgagcggaga
     4861 gcggcgtgtc aaccgcgtaa ggaaggctgt tgacggcccc ggtgagtcga agtacgactg
     4921 ggagatcatc tccatgctcg cgaagaagat gggcctcaag ggctttgact ggaagtctgc
     4981 aaaggatgtc tgggacgaca tgcgggcagt cactccaggc cagttcggcg taacctatga
     5041 aaagatggag aagcccgagt ctgtccactg gccctgcccg accatagaac atccgggaac
     5101 accgatcctc cacatcggaa aattctctgc agcggatggg aaaggcacca tgttcggtct
     5161 cgagtacagg cctcctgctg aagtggcgga tgcccaatac ccgttcaccc tgatgaccgg
     5221 ccgtgtcatt ttccactacc acaccaggac ccagaccgac cgtgccgcac aactgcacta
     5281 tgaagtgccc gagtcctata tccagatcaa taccgaagat gcaaagaaac tggggatcaa
     5341 ggaacgcgag aagatcaagg tttcgagtcg ccgtggtgaa attgaaactc ttgctcgtgt
     5401 aaccgatgat gttgcaccgg gcgtcctgta catggctatg cacttccaca agggtgcaaa
     5461 cacgctcacc aacactgcac tcgacccgct ctcaaagatg cccgagctga agcactgtgc
     5521 tgtgaaggtt gaaaagatag cggaggcgaa gtaaatggca ctctttggcg gaaaagccga
     5581 ggcaaaaaaa ggcgatatgc tctatgcatg gaccaatgat gcggatctcg caaagaaggc
     5641 ggaatgcggt ggggcggtga ctgagcttct caagtatgca ctcgagagca agacggttga
     5701 tgccgtgctt gccgtgaagc gcggtgttga cctgtacgat gcagtcccaa ccatcgttac
     5761 gaatcccaag gaccttgatg catgtgccgg gtccctccac tgcggtaccc tccttctctc
     5821 caaactggtg atggagtatg tgcccaagct ctctggcaag aagctcggaa tggtggtcaa
     5881 gggttgcgac ctgatgggcc tccttgagct ttcaaagcgg aaactggtca accttgacaa
     5941 tatcgtcatg atcggggtca actgcggtgg atcggtcagc ccggtcactg ctcggaagat
     6001 gatcaaggaa aagttcggcg ttgatcccaa cacggtcact aaggaagaga tcgacaaagg
     6061 ccagttcatt atcgagtatg agggaggcca caagggcatc tccatggatg aactggaaga
     6121 agccggttac ggccgccgat cgaactgtcg cagatgtaag ctgaaggtac cacggcaggc
     6181 ggacctcgcg tgcgggaact ggggtgttat cggcgataag gctggcaagg ccacgttcgt
     6241 cgaggtatgc tctgacaagg gtgcaaaact actcaacgcc gctgtcaaag ccaacaaact
     6301 aaccgctgaa ccagcaaacc agaaaggcat cgagctcagg accaaagtag agaattcgat
     6361 gtataagctc ggcgacaagt ggcgcaagca tgacttcgag gcactcgccc cgaacctctg
     6421 ggagacaatc gccaaggaga ccgggcgctg catgaagtgt tacgcatgca tcgaccactg
     6481 cccggtctgt gtcaccattg ctgacaagtg gaagcagccc gaccagatgg tccgtgcggg
     6541 tttcatccca gccgacccaa tgttccacct gcgccgcttc gcacacatct ccgactcctg
     6601 tatcaattgt gggcagtgcg aagagggctg cccgtcggag ataccgctgg cgctcttctc
     6661 gcatgctatc aggacagagg cagacaagtt ctttgagccg aagctcggga agccagccta
     6721 taccaattaa tttttcctgg cggaacgatg cacaaatctt aaaagggaga tgaaagatcc
     6781 atgaaagcag acgatgcaaa aaagatcatt aaggatgcaa tcgacaagga gatcgaggca
     6841 tacacgtttt accgtggcat tgccgacaaa gtgaaggaca aggcattgaa ggacctgttt
     6901 gcagaactcg ctggcgagga gaagaagcac cgtgagttcc tgcagggcgt gctcgataag
     6961 gatgtatcaa agatgaaatt tgatccatcg cacgattaca aggtggggga caacctgccg
     7021 acaccagaac tgaaagtcga gatgaaacct ctcgaaggtc ttgttctggc gatcaagaaa
     7081 gagctgcagg cgatgcagat gtacacccag ctcgcgaaac t
//
LOCUS       DAOOCO010000096         7100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_9914_length_7100_cov_4.948474, whole genome shotgun sequence.
ACCESSION   DAOOCO010000096 DAOOCO010000000
VERSION     DAOOCO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9914_length_7100_cov_4.948474"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PK154_06400"
     CDS             <1..737
                     /locus_tag="PK154_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10728.1"
                     /translation="TASTGLRAGHSRLLLQDEIKAELLRYFRTAHENLMEICEEHATL
                     TIDVAMVLRDSLIVIIRGEDGDYLTRNAMRAKRLESEADALVIKVRSLSRRIETATFY
                     ADLINVTDDALDFLEEAMFLSTLIPRVTSSKKLTGEMVDLAEIAVKSSQEFLKALMAS
                     QNIHRGYTRDDMQDFLQAVDRVIGYERECDEALRQSEKSILTQSTDYREMMIFFEIAR
                     TIEESTNSLMKAVYGIRDRILEGMNR"
     gene            744..2354
                     /locus_tag="PK154_06405"
     CDS             744..2354
                     /locus_tag="PK154_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPW10729.1"
                     /translation="MIPIAKSGHRLVKFGPGVRDEVSSAEFGNKASGLATMAQMGIPV
                     PPGFSLSLSICEEYYRKGKTLPDDFSSLLNEGIAFLEKASGSMFGGIRRPLLVSVRSG
                     APVSMPGIMDTILNVGLNRETLRGLIFLSGNPRFAWDTYRRFVLEFSRVVFRQDPTPY
                     HQALRTMLDAEGVTEPIELNSGSLKNLAFLYERIFSTGIGKRFPSDVHEQLACAAEGV
                     IRSWTHPRAEAFRQLNLVKEARGTAVTIQMMVFGNMGARSGAGVAFTRNPWLGTRGLL
                     IDFKFGAQGEDVVSGDQSAASQAEFARAMPDVFSALAEIGGRLEFHYRDMQDLEFTVE
                     EGRLYILQTRSGKRSSYAALKIAVALCQEGLISPADVLWMLRDVNLDSITVQEIKSGD
                     YPVAVGIPASAGIATGTIAFSGERAEAVAHQGIPVLLVRETASPDDLPGLSYARGLLT
                     RRGARTSHAAVVARQMGKICVVQCEALEIDYPRGRCTLSGHDLKEGSVITIDGSSGNV
                     YLGTVESTEKCPDELISIVRNWEREQQL"
     gene            complement(2360..3691)
                     /locus_tag="PK154_06410"
     CDS             complement(2360..3691)
                     /locus_tag="PK154_06410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW10730.1"
                     /translation="MIVVLGGGPAGRTAAITLALGGLDVTLIEKNGIGGQCLHYGCMV
                     VCALNEAARVLTSARRLEDLGVIENVPHIRFPALLYEMKAIQDKIAGILDAETRGSGV
                     SIVYGKEGRIDGTSVFIDGERFAADAVIAATGSHPAIPDVPGINTEGVYTPHTLGQMS
                     CLPDEMVIIGGGIMAAEFSYIFQEFGTQVHLVSRSGLLKNLDPKLIPMIRKELGNTSI
                     HENTSLISIEREETLSQVHLKGSKGAYEIPCDAVFIAAGLIPRSEMIHGIEKRSNGEI
                     VVDARMRTSAKGIYACGDVTGSPCLTPVARREGFVAAQNILGRDEVMDYAAVPQSMSL
                     FNEYAFVETDTSCAATASLPGPAGPGTFWGVPSGMTGFAKVSVDPETGELCGVESVGP
                     AAGIIAAYQAFLMRQGIGIYEFEKFFEIHPMTDGVYPLMKFMAGKLERKPP"
     gene            complement(3701..4651)
                     /locus_tag="PK154_06415"
     CDS             complement(3701..4651)
                     /locus_tag="PK154_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10731.1"
                     /translation="MDGKLRENAGVAVKSILETAGFEVQEVEAPFDFSAIREEEVLLV
                     LCSNDGAEIEEFDGTHFSLKVDDKEMECRKLVFTLEETIETENCIVWGVHEFVRYSGE
                     AALARVLERTLSLSFASVQKEVLSSKEIEPKEEAGSGITIPHLPIKVSMQAAEKIAGV
                     AGKPTLRFMPHWYFHYVSSGEQTYKDHKVLFDGEGVGAINAINGLRIELDGSDVAERE
                     APADSEVVKPKIAKEAAADQIFKDLVDMLTRQVRIKQVKGDAIFYEEKVIKPERKNIQ
                     LEVTSVYVPVWQVRGKKIIEVNATTGEILAVPMDEGVEIL"
     gene            4714..5460
                     /locus_tag="PK154_06420"
     CDS             4714..5460
                     /locus_tag="PK154_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HPW10732.1"
                     /translation="MNLPLKFVSHKIEEYALQVTYDPRKGSGRFVYNLSLIDSKDLER
                     SIAVYKDAFKAGVCVSGLVRFASSGEEIGDFTIPAEKTGIITVCSTTLDGILMHRGVP
                     LNPIGGGVVEIEQRIPRRFTHLILYQHTTIDPLQVLIAQEITSITDVMRKGSGTILAN
                     IRECHMEAEHLVGQVIDDLTGSPFTGILDIGPPNSPALGVQVSPQYMGIVALGGTNPM
                     AAIREAGIDVTIHAIKGLLDIGTMSEILDY"
     gene            complement(5468..6232)
                     /locus_tag="PK154_06425"
     CDS             complement(5468..6232)
                     /locus_tag="PK154_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW10733.1"
                     /translation="MAEPAAQTVALAESLKSLGLTKYEALVYIALLQASEATATEIHE
                     LSGVPRASVYPVLERLSQKQLVSVSNTSPRRFNPERPDDAIESLLSSVEHDAARAKTI
                     LNRIYSQSVRRARGDQELIWSIHGGDHIRLRLLEMLQAADENVRIIFYWDHLKGELVE
                     RLLSLKKEVKIDIITDGWSGPVQGQINVLVKAPPMEIQREPVTNWQAGCVFIVDKKKA
                     MVVMGSKDEGFTALYSESVGFVRFFTMYWNFFLNWG"
     gene            6297..>7100
                     /locus_tag="PK154_06430"
     CDS             6297..>7100
                     /locus_tag="PK154_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW10734.1"
                     /translation="MKIARFAILVVFIALMSSPAMAGTKYLSGGPDITAAIAGTNEFS
                     PGQDAAFKVNVENGGLIDIKFVQSGIVERDDLPNTAKLVRATLLPGDAPLTIKSDPQL
                     VGDIGGGKSVQVTFLAKVDENAPAGAYILPLVLEYTYLRNAEQYGQDSITYTYKEVNE
                     TIDLPITIKPEAFVAVESFSAEHLNAGNEGYLVMHLKNIGHVNASSAIVKIARNGNSP
                     IIPTDSSIFIGDFPINGTVDPRFKVSISSEAGGQSYPLDVYIVYEDESGS"
BASE COUNT         1722 a         1798 c         1794 g         1786 t
ORIGIN      
        1 agactgcctc gacgggtttg cgtgccggac attcgcggct gctgctccag gacgaaatta
       61 aggcagagct cctccggtat ttccggacgg ctcatgagaa tctcatggag atctgcgaag
      121 agcacgccac gcttaccatt gatgttgcca tggtactccg ggattccctt atcgtgatca
      181 taaggggaga ggatggggat tatctgacca ggaatgcaat gcgggcgaag cggttggaga
      241 gtgaggcaga tgccctcgtg atcaaggtcc gcagcctctc ccggcgtatt gaaacagcga
      301 cgttttatgc agatctcatc aacgtcaccg acgatgctct tgactttctt gaggaagcga
      361 tgttcctttc aacgcttatt ccccgggtga cctcgtcaaa gaaactgaca ggtgagatgg
      421 tggaccttgc ggagattgca gtgaaaagca gccaggaatt tttaaaagca ctcatggcat
      481 cgcagaatat ccacagggga tatacccgtg atgacatgca ggattttctc caggcggtcg
      541 acagggttat cgggtacgaa cgggagtgtg atgaagccct tcgccagtca gaaaagagta
      601 tcctgacaca atccaccgat taccgggaga tgatgatctt ttttgaaatt gcccggacga
      661 tcgaggaatc caccaactcc cttatgaaag cggtgtacgg catcagggat cgcatcctcg
      721 aaggaatgaa ccgttgagaa gagatgatcc ctatagcaaa atcaggccat cggctggtaa
      781 agtttggtcc gggtgtccgg gacgaggtat cttctgccga gttcggcaac aaagcgtcgg
      841 gccttgcgac aatggcacag atggggatcc cggtccctcc cggattctct ctctctctct
      901 caatctgcga agaatattac cggaaaggca aaacactccc tgatgatttc tcaagtctcc
      961 tcaacgaagg gatagctttt ttggagaagg caagtggttc gatgtttggg ggtatccggc
     1021 gccccctgct cgtttctgtc cgatcgggag ctccggtgtc aatgccgggg attatggata
     1081 ctatccttaa tgtaggacta aacagggaga cccttcgggg attgatattt ttatcgggca
     1141 atccacggtt tgcctgggat acctaccgcc gatttgttct cgagttttca agggtcgttt
     1201 tccggcagga tccaaccccc tatcatcagg cgctcagaac tatgctcgat gccgaaggtg
     1261 ttaccgagcc aatcgaactc aactccggga gccttaaaaa ccttgccttc ctgtacgaac
     1321 gtatattctc aaccggaatc ggaaagcggt ttccttccga tgtgcatgag caactggctt
     1381 gtgctgctga aggagtgatc cgttcctgga cccatccgcg tgcagaggcg ttccgtcaac
     1441 tcaacctcgt gaaagaggca agaggaacag cggtgactat ccagatgatg gtgtttggca
     1501 atatgggggc ccgttcggga gcaggagtag cttttaccag gaatccatgg ctgggcacca
     1561 gggggctcct catcgatttc aaattcggtg cacaggggga ggatgtggta tcaggggatc
     1621 agtcagcggc cagtcaggca gaatttgccc gggctatgcc ggatgtcttc tcggcccttg
     1681 cagagattgg ggggagactt gaatttcatt accgggatat gcaggatctc gaattcaccg
     1741 ttgaagaagg aagactttat attctgcaga cacgttccgg gaaacgatcc tcttacgccg
     1801 cgttaaaaat agccgtcgct ctatgccagg aaggactcat ctccccggcg gatgtattat
     1861 ggatgctccg ggatgtgaat cttgattcca tcaccgtgca ggagataaaa tcgggtgatt
     1921 atcctgtggc agtgggtatc cctgcatcag ccggtattgc aacgggcacg atcgcctttt
     1981 ccggagaacg agctgaagcg gttgcacatc agggaattcc tgttcttctt gtcagagaga
     2041 ccgcatctcc ggatgacctt ccgggtcttt cgtatgcacg aggactcctt acccgccggg
     2101 gtgcccgaac ctcccatgct gcagtcgtag caaggcagat gggaaagatt tgtgtcgttc
     2161 aatgcgaggc ccttgagatt gattatcctc gtggacgttg tacccttagt ggacatgatc
     2221 taaaggaagg atcagtgatc actatagacg ggagttcggg caatgtttac ctcggcacag
     2281 tagaatcgac ggaaaaatgt cccgatgagc ttatcagcat cgttcggaat tgggaacggg
     2341 aacagcaatt atagaccgtt cagggaggtt ttctctcaag tttgccggcc atgaacttca
     2401 tgaggggata cacaccgtca gtcatgggat gaatctcaaa aaacttttca aattcatata
     2461 tcccgattcc ctgccgcatc agaaatgcct gataggccgc aattatccct gcagccggtc
     2521 ctactgactc cacaccgcac agttccccgg tctcaggatc cacactcacc ttagcaaagc
     2581 cagtcattcc cgaaggtaca ccccagaacg tgcccggccc tgcagggcct ggaagggatg
     2641 cagttgctgc acaggaggta tcggtttcca cgaatgcata ttcattaaac aggctcatgg
     2701 actggggaac tgcagcgtag tccatgacct cgtccctgcc aaggatgttt tgagctgcga
     2761 caaacccttc acgccttgca acgggagtaa ggcagggaga accggtcaca tcaccgcatg
     2821 cataaatccc tttcgcactg gtcctcattc gtgcgtccac cacgatctcg ccgttgcttc
     2881 gtttctcgat tccatggatc atttcagacc gtgggataag gcctgccgcg atgaacaccg
     2941 cgtcacaggg tatctcgtag gctcccttgc tccctttcag gtggacctgg gagagggtct
     3001 cttccctttc gatggagata agggaagtat tttcatggat agatgtgttt cccagttcct
     3061 ttctgatcat agggatcagc ttcggatcaa ggtttttcag gaggccgctc ctgctcacca
     3121 ggtggacttg ggtgccgaac tcctgaaaga tgtatgagaa ctcggcggcc atgatgcctc
     3181 ctccaataat taccatctca tcagggagac aagacatctg gccgagagtg tggggcgtat
     3241 agactccctc tgtgttgatg cccggaacat ccggtatggc aggatgcgac cctgttgctg
     3301 caataactgc atccgctgcg aacctttccc catcaataaa aacggatgtc ccgtcaatcc
     3361 gcccttcctt cccatacacg atactaactc ccgaaccacg tgtttcggca tcgagtattc
     3421 ctgcaatctt gtcctgaatc gccttcattt cataaagaag ggcagggaac cggatgtgag
     3481 ggacattttc aataactccc aggtcctcca ggcgtcgtgc agaggtgagt actcgtgctg
     3541 cctcgttgag agcgcaaacc accatgcatc cgtagtggag gcactggcca ccgataccat
     3601 tcttttctat cagtgtgacg tcaagcccgc caagagcaag ggtgatagca gctgttctcc
     3661 ctgccggtcc gccgccaaga acgacaatca tgggagcgac ctagaggatc tccacaccct
     3721 catccatcgg gacagcgagg atctcccccg tagtagcatt cacttcaata atcttcttgc
     3781 ccctcacctg ccagacaggt acgtatactg atgtaacttc gagctggatg ttctttctct
     3841 ctggtttgat cactttctcc tcgtagaaaa tagcatcccc ttttacctgc ttgatcctga
     3901 cctgtctggt cagcatatcg acaaggtcct tgaatatctg atcagcggca gcctccttag
     3961 ctattttggg tttcaccacc tcggaatctg caggggcttc cctctcggca acatcactcc
     4021 cgtccagctc tattctgagt ccgttgatag cgttgatggc gcccactccc tcgccatcaa
     4081 ataaaacctt gtgatcctta tacgtctgct caccggaact gacataatgg aagtaccaat
     4141 ggggcataaa ccggagggtg ggttttcctg ctacccccgc tatcttttca gcggcctgca
     4201 tacttacttt tatggggaga tgtggtatcg tgattccaga ccctgcttct tccttgggtt
     4261 caatctcttt tgaggagaga acctccttct gtacagaggc aaacgagagg gagagggttc
     4321 tctcaaggac acgtgcaagc gcagcttcac cggagtacct tacaaactcg tgtacacccc
     4381 agacaatgca gttctctgtt tctatcgtct cttcaagggt aaatacaagt tttcggcact
     4441 ccatctcctt atcatcaact ttaaggctga agtgggtgcc gtcaaactct tcaatctcag
     4501 caccgtcgtt tgagcagaga acaaggagta cttcttcttc acgtatcgct gaaaaatcga
     4561 aaggagcttc cacctcctgc acttcaaaac ccgcagtttc cagtatcgac ttgactgcaa
     4621 ctcctgcatt ttcccgcaat tttccgtcca tcaaccatac atttccttca tcttatagaa
     4681 caatttttct attgcgtgat cgatgaatta gtgatgaatc ttcccctgaa gtttgtcagt
     4741 cataaaattg aagagtatgc attacaggtc acctacgatc ccagaaaggg ttccggcagg
     4801 tttgtgtaca acctgtccct gatcgattca aaagacctcg agagatctat tgccgtctat
     4861 aaagatgcat ttaaagctgg agtatgcgtg agtgggcttg tcaggtttgc ttcaagcggg
     4921 gaagagatag gggattttac aatccctgca gaaaaaacag ggattatcac ggtgtgcagc
     4981 acgacactgg atgggatcct gatgcaccgt ggggttccat tgaatcccat aggaggcggg
     5041 gtggttgaga ttgagcagcg aataccacgg aggtttaccc acctgatcct ctatcagcat
     5101 accacgatcg atcccctcca ggtgctgatc gcccaggaga taacatcaat tactgatgta
     5161 atgcgaaaag gaagcgggac tattctcgca aatatcaggg aatgtcacat ggaagctgaa
     5221 catcttgtcg ggcaggtgat agacgatctg acaggttcgc cttttaccgg tatacttgac
     5281 atcggacccc cgaatagccc ggcgcttgga gtccaggtca gcccccagta tatgggaatt
     5341 gtggcattgg gtggcaccaa tccgatggcc gccatcaggg aagcaggaat tgacgtgacg
     5401 attcatgcaa taaaagggct gcttgatatt ggaaccatgt cagaaatact cgattattga
     5461 ccgtttgtta tccccagttc aggaaaaaat tccagtacat ggtaaaaaaa cggacgaatc
     5521 ccacggattc tgaatagagc gcggtgaatc cctcatcctt tgaacccatc accaccattg
     5581 cttttttctt atcgacaata aaaacacacc cggcctgcca gtttgtcacc ggttctctct
     5641 gaatctccat tgggggggct ttcaccagta cattaatttg tccttgaaca ggtccggacc
     5701 agccatcggt gattatatca atcttcacct cttttttgag agagagcagt ctctcaacca
     5761 gttctccctt gagatggtcc cagtaaaaga ttatacgaac attctcatcg gctgcttgga
     5821 gcatctccag taatcggagt cggatgtgat caccaccatg gatgctccag atcaattcct
     5881 gatcccctcg tgctctcctt accgattggg aatagatccg gttcaggatc gttttggctc
     5941 tggcagcatc atgctcaacc gagctgagta gactttcgat cgcatcatcc gggcgttcgg
     6001 gattaaaccg tctgggtgag gtatttgaga ctgataccag ttgtttctgg gaaaggcgtt
     6061 ccagcacggg gtagaccgat gcgcggggaa ctcccgagag ctcgtgtatc tcggttgccg
     6121 tggcctctga tgcctggaga agggcgatgt ataccagcgc ttcatatttt gtaagcccca
     6181 gggattttag tgattccgct aatgccaccg tttgagcagc cggttcagcc attaaataca
     6241 tataaatagg ttccgctatt aaatgttgtt atcataataa caacaagtga aatattatga
     6301 aaatcgctcg ttttgcaatt ctagtggttt ttattgctct aatgtcctct ccggcaatgg
     6361 ccgggacaaa gtatttgtct ggcggacctg atatcacagc agctatcgcc gggacaaacg
     6421 agttctcacc tgggcaggat gcagccttca aagtaaatgt tgaaaacggt gggctcatcg
     6481 acatcaagtt tgttcagtcc gggattgttg agagggatga cctgcccaat accgcgaagc
     6541 tggtacgggc aacccttctt cctggcgatg caccactgac catcaaatca gatccccagc
     6601 tggtagggga tataggaggg ggaaaaagtg tgcaggtcac gtttttggca aaggtagatg
     6661 aaaatgcacc cgcgggcgca tacattctcc ccctcgttct tgagtatacg tatttgagaa
     6721 atgccgaaca gtacggtcag gacagtatta cctatacgta taaggaagtg aacgagacta
     6781 tcgacctccc tattacaata aaacccgaag catttgttgc ggtagaatcc ttcagtgccg
     6841 agcatctgaa tgctggaaat gagggatatc tggtgatgca tctgaagaat atcggccatg
     6901 taaacgcatc atcagctatt gtcaagattg cccgaaacgg caacagcccc ataattccga
     6961 ccgacagtag tatctttatt ggtgattttc cgatcaatgg aacggttgac ccgagattta
     7021 aagtctcgat atcaagcgag gctggtggcc agagttatcc gctggatgtc tacattgttt
     7081 atgaagacga atcgggatcc
//
LOCUS       DAOOCO010000097         7015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10090_length_7015_cov_5.212069, whole genome shotgun sequence.
ACCESSION   DAOOCO010000097 DAOOCO010000000
VERSION     DAOOCO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10090_length_7015_cov_5.212069"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK154_06435"
     CDS             <1..843
                     /locus_tag="PK154_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10735.1"
                     /translation="ASFLLKARKKGENPFNAVNSAQKTRNLPNTRLETFVFRTTGLER
                     YVEIQTERGIRFMTTDITDYPHFRFIQTMPSRYTGNSLGFIEWTGEKGGYAPHDAVQL
                     PHSPKKPDLPHLANIHGLDHAATRVKAIERNDAIIEFMELTTYSFDFAVFVRSLNSIT
                     SVARLSSDDFAMVFTSGIAPFRENTDPGPTEMFIRNYGTRVHHIAFQTDRVEDTYNAL
                     KKDGLEFLVALVGSEEEGLKQTFSLPSPHTMLVNEYIHRYQGFDGFFTKSNVEMLTRA
                     TGKQ"
     gene            909..1649
                     /locus_tag="PK154_06440"
     CDS             909..1649
                     /locus_tag="PK154_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10736.1"
                     /translation="MRFSWQVKLGLILVSISIAVYSIKFLLLKNPADTLNYVFNSLGF
                     LPISAFLVTIILNELLTRRSRRERLEKLNMVIGTFFSEVGTMLLAEISDCDPGLDGIR
                     KRLLVRDTWQDQEFDDLKSFLEGYHYAIDINKIEIQKLTIFLNEKRNFLLRLLENPVL
                     LEHQTFTETLRTVFHLTEELERRSGVGELPTTDLSHINGDLVRVYRNISIQWVDYMKY
                     LRKNYPYLFSLAIRTNPFDQEASVIVRK"
     gene            1747..2235
                     /locus_tag="PK154_06445"
     CDS             1747..2235
                     /locus_tag="PK154_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPW10737.1"
                     /translation="MLKKSVEDALNKQVNAEFYSSYLYLAMSAYFESYPMKGFAKWLR
                     LQANEEMAHGMKIYGYIIEAGGTPTFSAIDAPKTTWKSAQAVFEQVYEHEQKVTGLIH
                     SLMDLSIKEKDHATTNFLGWFVKEQVEEEANASEILAQIKIVGDIPGHLFWLDHQLGK
                     RE"
     gene            2383..3486
                     /locus_tag="PK154_06450"
     CDS             2383..3486
                     /locus_tag="PK154_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPW10738.1"
                     /translation="MSRDVFFAGLRARDPCQSSIAKLHALFEAAGFGDCIEKDAPTAI
                     KIHFGERGNDAYINPVFARQVVDKIREHGGKPFLTDSNTLYLGSRSNSLDHIITALEH
                     GFSYATVAAPILIADGITGKNFREVKIAKRHFNRVLVSGDIADVTSMFVLSHFKGHEP
                     AGFGGAIKNLAMGCAPPAGKQEQHSAKPIVAEERCIGCAKCLATCPSSAICLVDGKSV
                     IDKESCIGCFECMSICPEHAIDVDWETEIPQFIERLVEYAFGTSIGKKVGYFNFLTNI
                     TPDCDCLPWSDAPIVPDIGFLASRDPVAIDAASFDLVNKQSGIRGSALAANLNEGEDK
                     FQGLRKRTDAFRQIRYAEEIGLGSGSYRLVELY"
     gene            3690..5279
                     /locus_tag="PK154_06455"
     CDS             3690..5279
                     /locus_tag="PK154_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPW10739.1"
                     /translation="MNLRQPNANEALGTVNRSRDVVPMSGICTRCLDGCKGGCDIWLS
                     SFRGREVLYPGPFGEMTAGADKNYPIDYSHLNIQGYAMGAKGLPEGTEIGPDTAVFYS
                     VDTMTEYGWDKKVRMRLPIFTGALGSTDIARINWEHFAIGAAISGITIVCGENVCGVD
                     PALELDNKGKVTKSPEMDRRIDIYKKFHEGYGEMLVQMNVEDTRLGTAEYVSSKHNLD
                     TLELKWGQGAKCIGGEIKVKSLERAVELKKRGYIVVPDPSRPDMQVAFKAGAIKEFER
                     HSRLGFVSKEGFLEEVDRLHDIGFKRVTLKTGAYSAVELAMAIRYSAEAKIDLLTIDG
                     APGGTGMSPWPMMNEWGIPTFYIQSLAYEYCELLKKRGIRVPNIAMAGGFSDEPNVFK
                     AIAMGSPYVKAVCMGRGLMIPGMVGKNIEKWIKAGELPKTVSKYGTVPEEIFVTYEEL
                     KEKYGSKFKDIPMGAIGIYTYAQKFRIGLQQIMAGSRNFNIGSISRNDLMALTEDASK
                     VSGIPYVMDAYADEALTVLLD"
     gene            complement(5480..6730)
                     /locus_tag="PK154_06460"
     CDS             complement(5480..6730)
                     /locus_tag="PK154_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10740.1"
                     /translation="MAELTSWLKKNLASFEIDESLALNATRHLSKPEQAKLFSPEIEK
                     MRTAEGRWYEAIMYEMFLKMALESDEIRGLALKGVDVPHGSRQRIKLGQNGLFYSRCG
                     DITVRGNGQDLAEFDLLFIDNNQHIGFAEVVTSASDMKEFEQEIEYKRTLLGYLFDQK
                     KVTFILISSFDVTNFVVGRRLVRAPNTLNIQTASCEEIKSQIIDRKPTNRTIRPSRNE
                     KFILARDIPLCRPFDYERYHENEKARVFTWISSGNREGDCPVPGDDTGRLVKKILYGA
                     LYPSAIRSVCKNHEFIIRGERIDYKDIMNRYSKAVFATDLPDFDPIIYLRNRKKQEYL
                     KMVQNGDGNFKFERFTPAKVGFFLWLESLQPVLGARIMTGLIDAFSPNGSNHCKDPAN
                     GKHRAIMGRQNDPRRSSETYPNIR"
BASE COUNT         1870 a         1631 c         1682 g         1832 t
ORIGIN      
        1 gcctcctttc tcctgaaggc aaggaaaaaa ggcgaaaatc cttttaatgc ggtgaattca
       61 gcccaaaaaa caagaaatct tcccaatacc cgtctcgaaa cattcgtatt ccggacaacc
      121 ggactcgaac ggtacgtgga gatccagact gaacggggga tccggttcat gacaacggat
      181 atcaccgatt atcctcactt ccgttttatc cagacaatgc cctcgcgata taccggaaat
      241 tcactcggtt ttatagaatg gacgggagag aaaggcggct atgctcctca tgacgcagta
      301 caacttcctc attcccccaa aaaacctgac ctgccacacc tggccaacat ccacggtctc
      361 gaccacgctg cgacacgggt gaaagccatc gagcggaacg atgctattat cgaattcatg
      421 gagctcacca cctattcctt tgattttgcc gtctttgtca ggtccctgaa ttccatcacc
      481 agcgtggcca ggctctcaag tgatgacttt gccatggtct ttacctccgg gatagcaccg
      541 ttccgggaaa atacggatcc cgggccgacc gagatgttca tccggaatta tggaacaagg
      601 gtccaccata tagcattcca gactgaccgc gtcgaggata cgtacaatgc cctgaaaaaa
      661 gatggcttgg agttcctggt tgcactggtt ggatcagaag aggagggatt gaaacagacc
      721 ttttctctgc cctcccctca cacgatgtta gtgaatgaat acattcaccg ctaccagggg
      781 tttgatggat tcttcacgaa aagcaacgtc gagatgctga cgcgggcgac gggaaaacaa
      841 tagtttttac ggatacgcgt aacgaattct tttactatga tactaaccta gttttacgag
      901 gtatgttgat gcgattcagc tggcaggtca aactcgggct catcctggtc tcaatcagta
      961 tagcggtcta ttcgatcaag ttccttctcc tgaaaaatcc tgcagatacc ctgaactacg
     1021 tattcaattc acttggattc ctgcctatca gcgcgttcct ggtgaccatt atcctgaatg
     1081 aactgctcac gcgtcgttcg cggagggaac ggcttgagaa gctgaacatg gtcatcggca
     1141 ccttcttctc tgaagtgggt actatgctcc ttgccgagat atcggattgt gatccgggcc
     1201 tggatgggat caggaaacgg cttcttgttc gcgatacatg gcaggaccag gaatttgatg
     1261 acctcaagtc gttcctggag ggataccact acgctattga tatcaataaa attgagatac
     1321 aaaaactcac cattttcctc aacgaaaaga gaaatttcct cctccgcctc ctcgaaaacc
     1381 cagtactcct cgaacaccag acgtttaccg aaacgctcag gacggttttc caccttaccg
     1441 aggagcttga aagaaggagc ggggtgggcg aacttcccac aaccgatctc tcccatatca
     1501 atggagatct tgtcagggta tatcggaata tctcaataca gtgggttgat tacatgaaat
     1561 atcttagaaa gaactatccc tatctcttct cgctagccat acggacgaat ccttttgacc
     1621 aggaagcatc ggtgatcgtc cggaaataat tactctcttt ctcattgaag atggaattgg
     1681 tactataccc gttcgaaagg attaaaagaa aatacgccaa cattgcggtt aaaaaggaga
     1741 gaaaaaatgc tgaaaaagtc ggtcgaagat gcactgaata aacaggtgaa cgcggaattc
     1801 tattcatcat atctctatct tgccatgtct gcgtatttcg agtcgtatcc tatgaaggga
     1861 tttgcaaaat ggctccggct ccaggcaaac gaagagatgg cacacggaat gaaaatatat
     1921 ggctatatca tcgaagcggg aggaaccccc acattctctg ctatcgatgc cccaaagact
     1981 acatggaaat cagctcaggc ggtattcgag caggtctatg aacatgaaca gaaggtcact
     2041 gggcttatcc actctttaat ggatctttct atcaaggaga aggaccatgc tacaacgaat
     2101 ttcctgggct ggtttgtgaa agagcaggta gaggaagaag caaatgcaag tgagatcctt
     2161 gcacagatta aaattgttgg agatatcccg ggacaccttt tctggctgga ccaccagttg
     2221 gggaagcggg aataatcctc cattcatttt tatcggagat agtggtgatc gaatcccaaa
     2281 attttcaacc tgttacttat gcagccgcca gatctcttga aaattgttca tcattacata
     2341 gattattagg atggagacga acatcagaca cagaggatag aaatgagccg ggatgttttt
     2401 tttgcgggcc tgcgagcccg ggacccatgc cagagcagta ttgccaaact gcatgccctc
     2461 ttcgaagcgg ccggatttgg tgattgtatt gagaaggatg cgcctactgc tataaaaatc
     2521 cactttggtg aacgggggaa cgatgcctat atcaatcctg tcttcgctcg ccaggtagta
     2581 gacaagatcc gggagcatgg aggaaaacca tttctcactg attccaatac cctctatctg
     2641 ggaagccgat caaattccct cgatcatatt attactgccc ttgagcatgg tttttcatat
     2701 gcgaccgtgg ccgcacctat tctgatcgcc gatgggatta cgggaaaaaa tttccgtgaa
     2761 gtgaaaatcg cgaaacgcca ctttaatcgg gtactggtat caggggatat tgctgatgta
     2821 acctccatgt ttgtcctctc tcactttaaa ggtcatgagc ctgcgggatt tgggggggct
     2881 atcaagaatc ttgctatggg atgtgccccc ccagccggga aacaggaaca acattcggca
     2941 aaacccattg ttgcagaaga gcgatgcatc ggttgcgcaa aatgccttgc cacatgcccg
     3001 tcttcggcta tctgcctggt cgatggaaaa tccgtgatcg ataaagagtc atgcataggt
     3061 tgcttcgaat gcatgtcaat ctgccccgag catgcaatcg atgttgactg ggagaccgag
     3121 atcccacaat tcatcgagcg acttgtggaa tatgcctttg gaacatcgat cggaaaaaag
     3181 gtcggatatt ttaattttct gaccaatata acccccgatt gcgattgctt gccatggagt
     3241 gacgctccta ttgtgccgga cattggattc cttgcttcgc gtgatccggt ggcaattgat
     3301 gccgcaagct ttgatcttgt caacaagcag tcagggatca ggggttcggc gcttgctgct
     3361 aaccttaatg agggagaaga caagttccag ggattgagaa agagaacgga tgccttccga
     3421 cagatccgtt atgctgaaga gatcgggctc gggtctggtt cttatcgact ggttgaactc
     3481 tattgatctc tcttttctca gaacgctgat atctattttt ccagggcaaa cgacaggtag
     3541 ggtacaacca cctgtcaaca aaataactat atagtggagg aaggcaagcg cttataactg
     3601 ttcaaggggg acattaatgt tacgtttggg cagacaggat cccaaaccta cttcaatgct
     3661 gaatttgaaa tgagcaaaag gagagaagaa tgaacctcag acagccaaat gccaatgaag
     3721 cgctgggcac agtaaatcgt tcgcgcgatg tcgtccctat gtccgggatc tgtacacgtt
     3781 gccttgatgg gtgcaaggga ggatgtgata tctggctctc ttccttccgc gggagagaag
     3841 tcctttaccc cggacccttc ggcgagatga ccgccggtgc ggacaagaac tacccgattg
     3901 attattccca tttgaacatc cagggatatg ccatgggtgc aaagggactt cccgaaggca
     3961 cggaaattgg tcctgacacc gcagtatttt attccgtcga tactatgact gaatacggct
     4021 gggataagaa agtgaggatg cgcctcccta tttttaccgg agcactcggg tctactgata
     4081 ttgcccgtat caactgggaa cattttgcta tcggggctgc catctctggc atcactatcg
     4141 tgtgtggtga aaatgtctgt ggtgtggacc ccgcccttga gctggacaat aagggtaaag
     4201 tgacgaaatc cccagaaatg gaccggcgta ttgacatata taaaaaattc catgagggat
     4261 atggtgagat gctcgtccag atgaatgtcg aggacacccg ccttgggact gctgaatatg
     4321 tctcatcaaa acacaatctc gataccctcg aactcaaatg ggggcagggt gccaaatgta
     4381 ttggtggaga gatcaaggta aagtctcttg aacgggctgt cgaactcaag aagaggggat
     4441 acatcgttgt accagatccc tcccgtcccg atatgcaggt ggccttcaag gcaggggcga
     4501 taaaggagtt cgaacgccac tcccgacttg gatttgtgtc aaaggaagga ttcctcgagg
     4561 aggttgacag gctccatgat atcgggttca agagagtcac cctgaagacc ggggcttatt
     4621 ccgcagttga actcgccatg gccatccgct acagtgctga agcaaagatt gacctcctca
     4681 ctatcgatgg cgctcccggg ggtactggaa tgagcccctg gccaatgatg aacgaatggg
     4741 gtattcccac attctacatc cagtcccttg catacgaata ttgcgagctc ctgaagaagc
     4801 gcggtatccg tgttcctaat attgcaatgg ctgggggatt ctcagatgaa ccgaatgtgt
     4861 tcaaggccat cgccatggga agcccctatg tgaaagcggt ctgtatggga agaggtctta
     4921 tgatccccgg aatggtcggc aagaatatcg agaagtggat caaggcagga gaacttccaa
     4981 agacggtctc caagtatggt actgtccccg aagaaatatt tgtcacctat gaggaactca
     5041 aggaaaaata cgggtcaaag ttcaaggata tcccgatggg tgccattggt atctacacgt
     5101 atgcccaaaa gttccgtatc ggcctccagc agatcatggc gggaagcagg aacttcaata
     5161 ttggatcaat ctcgagaaat gacctcatgg cattgactga ggacgcttca aaagtgtcag
     5221 gtatccccta tgtcatggat gcctatgctg atgaagccct tacagtcctg ctcgattgaa
     5281 ccgtgtgaat tactatctgt ataccctttt ttatatgaaa atgcctccgg cattttcatc
     5341 ttttatgctt gtggccctcg aaggaatcgt gttattttta tataaaaatg cctccggcat
     5401 tttcatcttt tatgcttgta gccctcaaag gaatcatgtt atttttatga taaaattgaa
     5461 ctggctcttt gatcgggaat tatcgaatat tagggtatgt ttctgatgat cgccttggat
     5521 cgttttgtcg tcccataatc gccctgtgct ttccgttggc aggatctttg caatgattgg
     5581 atccgtttgg tgagaaagca tcgataagcc ctgtcattat ccgtgctccg agaacaggct
     5641 gaagagattc gagccagaga aaaaaaccaa ctttggctgg agtaaaccgt tcaaatttaa
     5701 agttgccatc cccgttctgg accatcttaa ggtattcctg cttcttccgg ttccgcaagt
     5761 aaataatcgg atcgaaatcc ggaagatcag tcgcaaagac cgcttttgag taacggttca
     5821 tgatatcctt gtaatcgatc cgctctccac ggataatgaa ttcatggttc ttacagacag
     5881 aacggattgc tgagggatac agggcgccgt aaagtatctt ctttacgagt cttccggtat
     5941 catcgcccgg gacaggacaa tcaccttctc tgttcccaga tgatatccag gtaaagacac
     6001 gtgccttttc attctcatgg taccgttcgt aatcgaatgg gcggcagaga ggaatgtccc
     6061 gggcgagaat aaatttttca tttcgggagg gtctaatagt gcgatttgtt ggttttctat
     6121 caatgatctg ggattttatc tcctcacagg aagcggtctg gatgttaaga gtattcggcg
     6181 ctctgacaag gcgtctcccc acaacaaagt tggtaacatc aaaagaggat atcaggataa
     6241 aggtgacctt tttctgatca aaaaggtacc caaggagggt tcgcttatat tcgatctcct
     6301 gctcaaattc cttcatatca gaggccgagg taaccacctc tgcaaatccg atgtgctgat
     6361 tgttgtcaat aaaaaggagg tcaaactcag caagatcctg accattgcct ctgacggtga
     6421 tatccccgca tcgtgaatag aaaagcccgt tttgccccag ttttatgcgc tgcctgctcc
     6481 catgtggcac atccactccc ttcagtgcaa ggcctctgat ctcatctgat tcgagtgcca
     6541 ttttgagaaa catctcatac attatggcct cgtaccatct cccttcggca gttctcatct
     6601 tctcgatctc gggagaaaag agttttgcct gctctggttt actcagatgt ctcgtggcat
     6661 tcaatgcgag actctcgtca atctcgaagg atgctaaatt ctttttaagc cacgaggtaa
     6721 gttctgccat ggaattatta caattattta ttttttttgg ttaaataagt accctgtagt
     6781 atcccaagta tccttcactc ctcgattacc actgcatccc aaagaatccg cttggtacga
     6841 aaatgatcat aagcaggcgt tgatttaatt gaaccattat tctcaatcct gatgcaagaa
     6901 caaatatgtt ataaaaatat atttatatag ttttcgcgca ttgtaataat gctgtacggt
     6961 gcgacagggt gggctcgtag ctcagttagg tagagcgcct ggcttttaac caggt
//
LOCUS       DAOOCO010000098         7008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10102_length_7008_cov_5.009924, whole genome shotgun sequence.
ACCESSION   DAOOCO010000098 DAOOCO010000000
VERSION     DAOOCO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10102_length_7008_cov_5.009924"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK154_06465"
     CDS             complement(<1..313)
                     /locus_tag="PK154_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPW10741.1"
                     /translation="MRTWKFGDNIDTDAIIPGRYLVYNDPLVLASHAFEGTRNDFAKG
                     VALGDVIIGGRNFGCGSSREHAPLALAGAGIRYVVAVSFARIFYRNAVNVGLLPLVCP
                     DA"
     gene            complement(316..1566)
                     /locus_tag="PK154_06470"
     CDS             complement(316..1566)
                     /locus_tag="PK154_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPW10742.1"
                     /translation="MGATIVEKIFSLKCGHSVRAGEVVMAPVDGAMIHDITGPLAIRN
                     FFEMGGKEVFDPEKIILLFDHQVPADSLAAAENHVFMREFARNQRIHNFDIREGICHQ
                     VVLEKGKAGPGEIIVGADSHTCMYGATGAFATGIGSTDMGFVLKFGALYFRVPETIRA
                     EVSGKFQRRVGPKDLILSIARDIGADGATYQAIEFCGETFARMPIHGRMTCCNMAIEM
                     GAKAGLVPPDEVTYEYMHSRGRIGRVSIESDPDAQYAARKKYDVTNLPPQVAIPHNVD
                     LARDVGEVAGTRVDQVFIGSCTNGRFEDLADAAEVIGDRKFDDSTRVIIIPASRDEYL
                     KALQAGLIEQFVKAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSADAS
                     VYLCSPATAAASALYGEITDPREV"
     gene            1725..2459
                     /locus_tag="PK154_06475"
     CDS             1725..2459
                     /locus_tag="PK154_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW10743.1"
                     /translation="MRERTVTLILVLLVVILALIPGVAMAADPACPENLITVSGNGEV
                     MTEPDIGLVTLGVRTEHADVRTAQEQNAMIMDEIITSLLAAGIPRQDIQTVSYNIYPV
                     YEEETRPFGGNVKYYQVTSMVQVTVREVSRTGEIIDIAVKNGANQVSSVGFSLSPEKE
                     KSLRATVLTQAVANARSDADVTAAATGVTITGVKSVVVGSVYVPVAYDNRYSGSAEMK
                     AVSVPTPIETGQIKVSAQVSISYLIT"
     gene            2858..3301
                     /locus_tag="PK154_06480"
     CDS             2858..3301
                     /locus_tag="PK154_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HPW10744.1"
                     /translation="MERNIGFKERRYIEELRILTKATALDCVIDERFERIIYVIKKGD
                     MGLAIGKKGENIRRMQNVLGKRIEMVEYAEDPHEFLTNIFRPVEIHSIQKDPGTGALL
                     VTIEKKSELGTAIGKKGANIEKARLLMRKFWGAELGEIRIGDETQ"
     gene            3298..3594
                     /gene="hisE"
                     /locus_tag="PK154_06485"
     CDS             3298..3594
                     /gene="hisE"
                     /locus_tag="PK154_06485"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPW10745.1"
                     /translation="MTLPERVLEDLWNVINDRFEHPKDSSYISCLVSTEKGSDKTLEK
                     IGEEATEFIIAAKNGDRERIISEAADLQFHFLIALRAYGVTFQEVIDELSSRRR"
     gene            complement(3598..4050)
                     /locus_tag="PK154_06490"
     CDS             complement(3598..4050)
                     /locus_tag="PK154_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPW10746.1"
                     /translation="MPPVPCRVKGVFVARNALGVSPAVILSAENEHCLPIYIGLFEAM
                     SIHSALNNEIPPRPLTHDLFVELLKQFDVSLKALVIDALEDGVFYATMVLFKDHHELK
                     IDCRPSDGIAIGIRCQAGIMVEEEVILQSAVEAGDLPELVDIGSYLAG"
     gene            complement(4092..5111)
                     /locus_tag="PK154_06495"
     CDS             complement(4092..5111)
                     /locus_tag="PK154_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HPW10747.1"
                     /translation="MDRNQKRWLWISLGFSAVVLVAVLYFTVDAATVSYLRRLNPFWL
                     ILALCTHVIALCFWALRIKFMSRSLGYRVGFFYSLNLVFANMLLAAITPSQAGGEPVR
                     IHELYKAGVKVGDATAIVLTERILDGIVLGIGGAFFMFVLGSEWRRIGSVFSYLMYIS
                     WIIITIMVIIFIYSVKNPGLLKRIIHYSSRWFTRKWESRKIERFAQSIDSEVDNFHNT
                     LADFLSHGKAGLIFGLIFTTLFWFCEFVIVSILLVGLSQPPIFIESLIVQLVIAVIMM
                     IPLTPGASGIAEISFTSLYGLFVNSSILGILVVLWRSILFYFNILLGLISGLLIVQRE
                     ARHPD"
     gene            complement(5205..5777)
                     /locus_tag="PK154_06500"
     CDS             complement(5205..5777)
                     /locus_tag="PK154_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HPW10748.1"
                     /translation="MSQADCRDALLRRMNGIRIAAVEGTYLFSSGGVFREMADAYLTD
                     GDRFQSLGDLMNAYASYWYALGWLDAGTALGLIFTPLPGFSPDFSCGTRSDPDTIRVQ
                     EKTTRYRSLLARALESLDNAPEKDTSLFAAADHFRETAISTYERGISLDEEDALAGAL
                     AAYSYGFAWLDAGVRFGLFRIVRNREIFTI"
     gene            complement(5774..6523)
                     /gene="dph5"
                     /locus_tag="PK154_06505"
     CDS             complement(5774..6523)
                     /gene="dph5"
                     /locus_tag="PK154_06505"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HPW10749.1"
                     /translation="MLTFVGLGLFDLEDISVKGLECIRTADILFLEAYTSKLAGCTVK
                     EMENRYGKEIQILSRHDVEVDPDPLLELAATSRVVLLVGGDPMVSTTHIDLRIRARMR
                     GISTRIIHGSSISTAICGLSGLQNYRFGKSCSLPFPHGSWFPMTPINVIGENIRQDLH
                     TLVYLDIQDDRFMTIHKAVTLLEEMAARSGGHIPLYVGIARAGSDSPVVKAGDARTLL
                     ATDFGSPLHIIAIPASLHVMEREYLELFGGL"
     gene            complement(6655..>7008)
                     /locus_tag="PK154_06510"
     CDS             complement(6655..>7008)
                     /locus_tag="PK154_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPW10750.1"
                     /translation="LFQYLGFMAAFEAFIAPFSETVLGLPAYATTALIFGILRKEMAL
                     ETLIILAGTADLGSVLSMAQIYIFAVVSVLFVPCIATIAVLRKEVGTRMAIVIAAYTL
                     ALGILAGAVLNLLLS"
BASE COUNT         1736 a         1887 c         1868 g         1517 t
ORIGIN      
        1 cagcgtcagg gcacacgagg gggagaagcc ccacgttcac ggcgttccgg taaaatatcc
       61 gggcaaatga tacggcgacc acataccgga tgcctgcccc ggccagtgcc aggggagcat
      121 gctcccgcga tgatccgcat ccgaaattcc ttccgccgat gatgacatcc cctaaggcaa
      181 ctccttttgc aaagtcgttt cgtgtcccct cgaatgcatg cgacgcgagt accaatggat
      241 cattgtacac caggtaccgc cctgggatga tggcatcggt atcgatgttg tccccgaact
      301 tccaggttct catggtcaaa cctccctcgg gtcggtgatt tcaccataaa gggcgcttgc
      361 ggcagccgtt gccggtgaac agagatacac cgaagcatcg gcactcccct gcctgcccct
      421 gaagttcctg ttcgaggtcg agagggaaac ctccccggga gcgagcagtc caaaggcacc
      481 gcccatacag gggccgcaac agggggcttc caccagcgcc ccggccttca cgaactgttc
      541 gatcagccct gcctgcagcg ctttcagata ttcgtcccgc gatgcgggaa tgatgatgac
      601 gcgggttgag tcatcgaact tcctgtcccc tatcacttct gcagcatctg caaggtcttc
      661 gaatctcccg tttgtgcagg acccaatgaa tacctggtct accctggtcc ctgcgacctc
      721 tccaacgtca cgtgcgagat cgacgttatg ggggatggca acctgaggcg ggagattggt
      781 cacgtcgtac ttcttccggg ctgcatactg tgcgtcagga tcactttcga tcgacacccg
      841 gccgatcctt cctctgctgt gcatgtactc gtaggtgacc tcatcagggg gcacgagacc
      901 ggcctttgcc cccatctcaa tcgccatatt gcagcaggtc atcctcccgt ggatcggcat
      961 ccttgcaaac gtctccccgc agaattcgat agcctggtag gtggcaccat cagccccaat
     1021 atcccttgct atcgagagga tcaggtcttt gggaccgacc cgcctctgga atttcccgga
     1081 cacctcagcc cggatcgtct cgggcaccct gaagtaaagg gccccgaatt tgagcacgaa
     1141 tcccatgtcg gtggatccta tccctgtggc aaatgccccg gtagcaccat acatgcaggt
     1201 atgcgagtcg gcgcctacaa taatctcacc cggtcctgcc tttccctttt cgagaacaac
     1261 ctggtggcag atcccttcac gaatatcaaa attatggatc cgctggttcc gcgcaaactc
     1321 tcgcatgaaa acatggttct ctgccgcggc aagggaatcg gcagggacct gatggtcgaa
     1381 caggaggatg atcttttcag ggtcaaagac ctcctttcct cccatctcaa agaaattccg
     1441 aatggcaagt ggtcccgtga tatcatggat catcgcacca tccacgggtg ccatcactac
     1501 ctcacccgcc ctgaccgaat gtccacattt taacgagaaa attttctcta cgatggttgc
     1561 tcccatgagg gttcccagta tcaatgggac atgaagagat atggaagttg tggcaccaga
     1621 agcagggagt ctaacagcgt gattcctggg taactgcgaa atatttcgca ttaaaagata
     1681 attatttgaa ttttcccata gaatttctgc tgtggtgaat tacgatgcga gaaagaacag
     1741 tgacgctcat tctggtacta ctagtggtga tccttgcact gataccagga gttgcgatgg
     1801 ctgctgatcc tgcctgcccg gaaaacctca tcaccgtctc gggcaacggg gaggtaatga
     1861 ccgaacctga tatcggtctt gtgacacttg gtgtcaggac tgagcatgct gatgtcagga
     1921 ccgcacagga acagaatgcc atgatcatgg acgagataat cacctctctt cttgctgccg
     1981 gaataccccg ccaggacatc cagacagtaa gctacaatat ctaccctgta tacgaggaag
     2041 aaacccggcc gtttgggggg aacgtcaagt actaccaggt gaccagcatg gtccaggtga
     2101 cagtgcggga ggtatcaagg acaggggaga tcatcgatat cgcggtgaag aatggtgcaa
     2161 accaggtaag ttccgtcgga ttctccttgt cccccgagaa ggagaagtcg cttcgcgcaa
     2221 cggtcctgac ccaagccgtg gcaaatgccc gctctgatgc cgatgtgact gcggcagcaa
     2281 ccggtgtcac catcactggc gtgaaatcag ttgtagtggg atccgtgtat gtccctgtgg
     2341 cgtacgacaa ccggtacagc ggttcagctg aaatgaaagc cgtatccgtc ccaaccccga
     2401 ttgagaccgg gcagataaag gtatcagccc aggtatcgat ctcctatctg atcacgtaac
     2461 ctcctccctt tttgttgttc cggaccggat tgtagtccct cccccggccg tgcgaggtct
     2521 cttcccaggt gatcggatga agcctccttc gcctgtgaat aagggacaac agaccctttc
     2581 aggaaacggg atcggtgggc agtctgccag tcctttgagg agcataaccg aaagccaacc
     2641 ccttcgggca aacagagggc atttcctgtt cccttgtagt ccaggaaaac gcggtctttg
     2701 gaacattttc gtaattgcgc tcactctctt ttgtacacca tgcatgtttt tgagtaccct
     2761 gacctttccg gtatggatag gcgtaccagg aacccggaag aagaagcgga aataccttta
     2821 atcatgcccc ctgtatacac tatgaaggat tctgatgatg gaacggaata tcggcttcaa
     2881 agagcgacga tacatcgaag aactccggat cctgacgaaa gcgaccgctc tggactgtgt
     2941 catagacgaa cggtttgaac ggatcatcta tgtaataaaa aaaggcgata tgggtcttgc
     3001 aatagggaaa aaaggtgaga atatccgccg aatgcagaac gtccttggaa agcggatcga
     3061 gatggtggaa tatgctgagg atccccatga gttcctcaca aatatctttc gtccggttga
     3121 gatacattcc atccaaaagg atccgggaac gggggcactg ctggtcacca tcgaaaaaaa
     3181 gagcgaatta ggtactgcaa taggaaaaaa gggtgcaaac attgagaagg cacggctgct
     3241 catgcggaag ttctgggggg ctgaactggg cgagatcagg atcggtgatg aaacgcaatg
     3301 accctgccgg aacgcgtact tgaggatctc tggaacgtta tcaatgaccg gttcgagcat
     3361 cccaaagatt cgtcgtatat ctcctgtctt gtctcgactg agaaaggatc cgacaagacc
     3421 cttgaaaaga tcggcgagga agcaacagag ttcattatcg ccgcaaagaa tggagaccgg
     3481 gaacgaatta tctctgaagc agcggatctc cagtttcatt ttctgatcgc cctgagagcc
     3541 tacggggtca cgtttcagga agtgatcgac gagctctctt cgcggagaag ataacagtta
     3601 tcctgccagg tatgagccga tatccacaag ttcaggcagg tcacctgcct cgacggctga
     3661 ctgcagaata acctcctcct cgaccatgat cccggcctgg catcgtattc ctatcgcaat
     3721 accatcactt ggcctgcagt ctatcttcag ctcatggtga tccttgaaga ggaccatggt
     3781 ggcatagaat accccgtcct cgagcgcatc gatgacaagc gctttgaggg agacatcaaa
     3841 ctgcttcaac agttcgacaa agagatcatg cgtgagggga cggggtggga tctcgttgtt
     3901 gagggccgaa tggatggaca ttgcctcaaa aaggccgatg taaataggaa ggcagtgttc
     3961 gttttcggcc gacagtatga cggctgggga gacccccagc gcattcctgg cgacaaaaac
     4021 ccctttcacc ctgcatggaa ccggtggcat gactgcaggg atgattggtg catggcataa
     4081 aaaaggtttg ttcagtctgg gtgccgcgcc tccctctgaa cgatcagaag gccggagatc
     4141 agcccgagca ggatattgaa atagaagagg atcgatcgcc agagaaccac cagtatccca
     4201 aggattgatg agttcacaaa gaggccatac aacgaggtga atgagatctc cgcaattccc
     4261 gatgcacccg gggtgagcgg gatcatcatg atcacagcaa tgacgagctg gacgatgagc
     4321 gattcgataa agatgggcgg ctgggagagg cctaccagga ggatggatac aatcacaaac
     4381 tcgcagaacc agaagagcgt ggtgaatatc aggccaaaga tcagtccggc ttttccatgg
     4441 ctgagaaaat cggcaagggt attatggaaa ttatcgacct cactatcgat agactgggcg
     4501 aaccgttcga tctttctgct ctcccatttc cgggtgaacc agcgggaaga atagtggatg
     4561 atcctcttca acagaccggg attcttcaca gagtagataa aaatgatgac catgatggtg
     4621 atgataatcc aggatatgta catcagatag ctgaagacgg atccaattcg ccgccattca
     4681 ctccccagga cgaacatgaa aaatgcaccc ccgataccga gcacgatccc atcgaggatt
     4741 ctctcggtaa ggactatcgc ggtggcatcg ccaactttta ccccagcctt gtacagctca
     4801 tggatcctca ccggttcacc gcccgcctgt gagggggtta tcgcggcaag cagcatgttg
     4861 gcaaacacca ggttgaggga gtagaagaaa cccacccggt atccgagtga cctgctcatg
     4921 aacttgatcc ggagcgccca gaaacacagg gcgataacat gggtgcagag tgcgagaatg
     4981 agccagaacg ggttgagtcg gcgcaggtag gagaccgtcg ccgcatcaac ggtgaaatac
     5041 aggacggcga ccaggaccac ggcagagaat ccgagtgaga tccataacca tctcttctga
     5101 ttccggtcca tagataccga tcttctccct gagatatggc agattcatta gtggttactg
     5161 cgcatagtat aaacctttta aggaagagat tccggcgtaa ttcctcatat cgtgaatatc
     5221 tcccggtttc tgacgatcct gaacagcccg aacctgaccc cggcatcgag ccaggcaaac
     5281 ccatagctgt atgccgcaag agcacctgcc agtgcatctt cctcgtcaag ggagatacct
     5341 ctctcatagg tggagatcgc agtttccctg aaatggtccg ccgctgcgaa gagcgaggta
     5401 tccttctcgg gagcgttatc gaggctttca agcgctcttg caagaagtga acgataccgt
     5461 gttgtcttct cctgcacgcg gattgtatca ggatcggatc gcgtgccgca ggaaaaatca
     5521 ggtgaaaatc cgggcaacgg cgtgaatatc agaccgaggg ctgttcccgc atcaagccag
     5581 ccgagtgcat accagtagct ggcataggca ttcatcaggt cacccaggga ctggaaacgg
     5641 tccccatcag taaggtaggc atcagccatc tcgcggaata cccctcctga agagaacagg
     5701 tatgttccct ccacagccgc tatcctgatc ccgttcattc tcctcaggag ggcatccctg
     5761 cagtcagcct ggctcatagt cctccgaaca gctcaagata ctcccgctcc atcacgtgga
     5821 gcgaggcggg aatggcaatg atatgcaatg gagagccaaa gtctgtcgca aggagggtgc
     5881 gggcatctcc cgcctttact accggggaat cggatcccgc ccgggcaata cccacataca
     5941 gcgggatgtg ccccccgctt cgtgccgcca tctcctcaag aagggtgacc gctttgtgga
     6001 tggtcataaa acgatcatcc tggatgtcca gatacacaag ggtatgcaga tcctgcctga
     6061 tattctcccc tataacgttg attggagtca tgggaaacca tgatccatgg gggaagggga
     6121 gcgagcatga ctttccgaac cggtaattct ggaggccgga gagtccgcag atggcggtgg
     6181 atatggagga tccatggatg atccgtgtac tgatccctct catccttgcc ctgatacgca
     6241 ggtcaatgtg ggtggtcgag accattggat caccgcctac aagaagaacc accctgcttg
     6301 ttgccgccaa ttcgagcaat ggatcgggat ccacctcgac atcatgccgg gagaggatct
     6361 ggatctcttt tccgtaccgg ttctccatct ccttgacggt gcatcctgcc agctttgagg
     6421 tatatgcctc gagaaagagg atatctgcag tcctgataca ttccagccct ttgaccgaaa
     6481 tgtcctccag gtcaaagagt cccaatccca cgaacgtgag catgctgccc tcccgtcccc
     6541 gcaccattcc ggaaaatgtt ctggtccatg ttctgtgcca cacctgttcc tggatccaat
     6601 ggccaatacc tatcgatatg gtcgtccatc ttaataggta tgccccggat cccgctatga
     6661 tagcaggaga ttcaggactg cccctgcgag gatgccgagg gcaagcgtgt atgctgcaat
     6721 cacgatagcc atccgggtcc ctacctcctt tctcagcacg gcgatggtgg cgatgcaggg
     6781 gacaaacagc acgctgacca ctgcaaagat atagatctgt gccatgctga ggaccgaacc
     6841 gagatcagcg gtcccggcaa ggatgatcag ggtttcgagg gccatctctt ttctgagtat
     6901 accgaagatg agtgccgtag tggcataggc gggaagacca aggacggttt ccgagaaggg
     6961 cgcaataaaa gcttcgaatg ccgccatgaa tcctaaatat tgaaagag
//
LOCUS       DAOOCO010000099         6870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10347_length_6870_cov_4.977109, whole genome shotgun sequence.
ACCESSION   DAOOCO010000099 DAOOCO010000000
VERSION     DAOOCO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10347_length_6870_cov_4.977109"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="PK154_06515"
     CDS             complement(<1..1595)
                     /locus_tag="PK154_06515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B DNA-directed DNA polymerase"
                     /protein_id="HPW10751.1"
                     /translation="MWITDTCSRETVEIWDLGRDGTVRHRSVPYTPSFYLRLPDPPAH
                     WELLDALESAYGAEECTFRTVHGTCEGYRVPAGREVALAIERQTGCAAELYNVDLRPD
                     HQYLAAQDLVPCCDDPSSRFSPDLPALPFRTIEVHITGIPFEKDPVCSIEVVHERHEH
                     LRGEEGVVLADLADLFGAIDPDVVLFPRSDLWLPRLLSSARERGIMLPFSRTGRYRKM
                     TEKSYWSYGRTEYRAGSLIPDGRILIDTAQSFHYRESGLSGILLGARLTGLSPNLAAR
                     FTSGTLISAYEVYEALKRGIAIPFRKSDPEAVRRFCDLKSADRGGMMFQPVAGLYGQV
                     SEIDFTSLYPSIIVRYNLSPETLTHPDRRGFLPQVLGPLLLLRRETKARKAADPAYAG
                     IDAILKWMLVTCFGYTGYKNAKFGQIEVHEKITWHAREILLTTKECAEEMGFDVLHGI
                     VDCLWVQGGPAGALKARIEQVTGLHTTLDCYDWIVFLPMPDGYGAYNRYYGRLSTGDL
                     KIRGIMARRGDTPPYVRTMQEAML"
     gene            complement(1582..1818)
                     /locus_tag="PK154_06520"
     CDS             complement(1582..1818)
                     /locus_tag="PK154_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10752.1"
                     /translation="MGRSFESVRMGVKDITGNWEQVARSLPGREGVAALTVVAMARKH
                     ASEGFYIFDDPLEAALFSACIGILKAAEEQHVDH"
     gene            complement(1818..2345)
                     /locus_tag="PK154_06525"
     CDS             complement(1818..2345)
                     /locus_tag="PK154_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10753.1"
                     /translation="MEFDLHPTVTLRTGTFAALIAPEDLVLAGLNDNPVLRRFLFLFV
                     SGNYSRLLSGINRRSVTIEVRRAFTAFQILTILRDSYHTIIFVEHDPGLYEGSGKEAL
                     FQVAQALKGAGQNAAVVLYAPSPDPSFSLLADRADRFFSLSPPDWPALPRPRRSGRVR
                     HPAGLPGDQTTLEGI"
     gene            complement(2561..2695)
                     /locus_tag="PK154_06530"
     CDS             complement(2561..2695)
                     /locus_tag="PK154_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10754.1"
                     /translation="MPTCADCFLYTAKNGKEGECTINGRVPADRDAGRCPSRTFRPKA
                     "
     gene            complement(2734..3531)
                     /locus_tag="PK154_06535"
     CDS             complement(2734..3531)
                     /locus_tag="PK154_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPW10755.1"
                     /translation="MAEILQYIPGDGIFHRLHPVTKILFIIIVSVVTILTSSISLLAG
                     LLLLIFLLAYAGHLLRPVIQQMVLILLMSIVIYLITILTVQDGAVIGYLIPSFIPLIG
                     GSIPITIGALTVGTVLTLRFAILISAFQLFVISTQPRDLVHTMERARVPVDYTLMFII
                     ALRFIPTLQIEGKRIHEAQLARGYHSGDGFMGRIRSVAPVMVPLVSNSLSRATLLGLT
                     IDLRGYRTRARTRLRERTFGIADKVAIPLMCLAGIAFLYGFLTSSLL"
     gene            complement(3524..4963)
                     /locus_tag="PK154_06540"
     CDS             complement(3524..4963)
                     /locus_tag="PK154_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPW10756.1"
                     /translation="MITLENVSYAYPRADREAVSEITLTLRRGECVLVTGHSGSGKTT
                     LCLAAAGILEHEYGGKKTGRVTVDGKDVREYHDLTDISTRVGILFDDPDSQLLFTTVE
                     EEVLSAIERRGFSVAEVEERLAQVLAVTGLEELKDRPPHALSGGQKQRVVLATTLALG
                     TDILILDEPTSELDEQGTESIVRLLEEFKAQGKAILIVEQKFTKLASLVDRIVVLENG
                     RIRNEGPPATVLQDEIARRTLIPDFSGIRTRSPAVNGSAPMVCTRGLTFSYGEEEALS
                     GVDLSIAQGEFVAIVGENGSGKTTLVKHFIGLLRPNKGTVTVDGKDAAKAPIAELAHS
                     VGLVFQNPDHMFFADSVFDEVAFGVDNLGIPGREQAVMDALATVRLTAARDLYPRWLS
                     RGERQRLAIACVLAMQPAVLILDEPTTGLDGSESREILEILKQLQEKGHTIIMITHSR
                     EIAGQCADRVIHMEAGRIADDVRAEAAHG"
     gene            complement(4960..5487)
                     /locus_tag="PK154_06545"
     CDS             complement(4960..5487)
                     /locus_tag="PK154_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10757.1"
                     /translation="MKSRDIAIVGILLAVGAILRYFLAMLHTPLTPNMVIAFYCLAII
                     LVRPKVYEALGIGIVAGILSMLISSSIFPPANLISEPVGALVCFGLYTVLRNRTQLAP
                     TISTFLTTLASGFTFAAVAIMFVSATILAKYNGDMMGFVVAFVPIVVITAVFNAVIVQ
                     ILYYPASRVLMRGQE"
     gene            5782..6594
                     /locus_tag="PK154_06550"
     CDS             5782..6594
                     /locus_tag="PK154_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10758.1"
                     /translation="MLIADGEREQSMAEWKYCLLLLLLLGIAAGPAAAHPPSGMTLVY
                     SEQNGEVAMTVNHSVSDPSTHYIGEVTIRKNGGMVIGEVYSGQPSGDTFTYRYPIILT
                     PGDEIVATAECNIGGSGTARFLMPGQTVPAPSGPDTVPRYVYHAVLMVAGILCIIASG
                     LIPVYGKRIAGWYRLHVVTAAIGSILVIPALFLVFRVSYLSLSPSAFAIHVILGLLLL
                     LTLIATIVLSLIRNRMGPRKRLFRNAHIWMGRVFIILMVVNVLTGLAAVGVI"
BASE COUNT         1374 a         1945 c         2154 g         1397 t
ORIGIN      
        1 agcatggcct cctgcatcgt ccggacataa ggtggggtat ctcctcgccg tgccatgatc
       61 ccacggatct tcagatcgcc cgtgctgagg cgcccatagt agcggttgta ggcaccatac
      121 ccgtcgggca tggggagaaa gacgatccag tcatagcaat cgagcgtggt gtgaaggccg
      181 gtcacctgct cgatacgggc tttgagcgcc ccggccggcc ccccctgcac ccagaggcag
      241 tcgacgatcc cgtgcaggac gtcaaagccc atctcttctg cacactcctt tgtcgtgagc
      301 aagatctccc gggcatgcca ggtgatcttc tcgtgcacct cgatctgccc gaactttgca
      361 tttttatatc cggtgtaccc gaaacaggtc accagcatcc acttcaggat cgcatcgatc
      421 ccggcatagg cgggatcggc tgctttccgg gccttggtct ctctccgcag aagaaggagc
      481 ggcccgagca cctggggcag gaacccccgc cggtccgggt gggtgagcgt ctcgggggag
      541 aggttatacc ggacgatgat ggaagggtag agggaggtga agtcgatctc tgacacctgc
      601 ccgtagagcc cggctacggg ctggaacatc atcccgccac gatcggctga cttcaggtcg
      661 cagaacctgc gcacggcctc gggatcgctc ttacggaacg gaatggcgat cccacgcttc
      721 agtgcctcgt agacctcgta cgccgagatc agggtcccgg aggtaaaacg ggcagcgagg
      781 ttgggggaga gcccggtaag gcgggcgccg aggaggattc cggaaaggcc gctctctcgg
      841 taatggaagc tctgggcggt atcgatcagg atcctgccat cagggatgag ggagcctgcc
      901 cggtactcgg tcctcccgta actccagtat gatttctcgg tcatcttccg gtacctcccg
      961 gtcctgctga acgggagcat gatccctctc tccctggccg aagagaggag gcggggcaac
     1021 cagaggtccg agcgggggaa gagcacgacg tcagggtcga tggcaccgaa aaggtcggca
     1081 aggtcggcga ggaccacgcc ctcctccccc ctgaggtgtt catggcgttc gtgtaccacc
     1141 tcgatcgagc agacagggtc cttttcaaag ggaatgccag tgatatgcac ctcgatggtc
     1201 ctgaacggga gagcagggag atcgggcgaa aacctggacg atggatcgtc acagcagggg
     1261 acaaggtcct gtgcagccag gtactgatgg tcgggtcgca gatcgacgtt gtagagttca
     1321 gcggcgcacc cggtctgccg ttcgattgcc agcgcgacct cccggccggc tgggacccgg
     1381 tacccctcgc atgttccgtg cacggtccgg aaggtgcact cctcggcccc gtacgctgat
     1441 tccagggcat cgagcagctc ccagtgtgcc ggggggtccg ggaggcgcag gtaaaacgat
     1501 ggggtgtagg gcacactccg gtgccggacc gtcccgtccc ggccgagatc ccagatctcc
     1561 acggtctccc gggagcaggt gtcagtgatc cacatgctgt tcctccgcgg ctttcaggat
     1621 cccgatgcat gcggagaaga gtgccgcttc gagcggatca tcaaagatgt agaacccctc
     1681 gcttgcatgc ttcctggcca tggcaacgac ggtgagtgcc gcaaccccct ctcttcccgg
     1741 gagtgaccgg gccacctgct cccagttccc ggtgatatct ttcaccccca ttcgaacgct
     1801 ctcaaagctc cgccccatca gatcccctcc agggtggtct ggtcccctgg gagcccggcc
     1861 gggtgccgca cccgcccgct gcgacgtggc cgtggcaggg ctggccagtc gggtggtgag
     1921 agggagaaga accggtcagc ccgatcggca aggagggaga aggatgggtc aggggacggg
     1981 gcgtacagca cgaccgcggc gttctgccct gcccctttca gtgcctgtgc cacctggaag
     2041 agggcctcct tcccggatcc ctcgtacagc cctggatcat gctcgacaaa gatgatggtg
     2101 tgataggagt cgcggaggat ggtgaggatc tggaacgcgg tgaaggctct cctcacctcg
     2161 atcgtcaccg acctgcggtt gatcccggac aggagcctcg aatagttccc gctcacgaag
     2221 agaaagagga accgccggag caccgggttg tcattgagcc cggcaagcac gagatcctcc
     2281 ggtgcaatga gtgccgcaaa ggtcccggtg cgcagggtga cggtggggtg caggtcaaac
     2341 tccattccat ctccaggtgc accttccagg ctccataagg gcacagagca cgtggcgtga
     2401 aagtgcgatc gataggttca agcactggga aaaatccggt taaaacgggt ggaataagaa
     2461 aaaaagccat ctaaatgaat aactacgcga taaggcccct ttatctgccg gcacattcgg
     2521 ggagccatgg tggttccgtg tcctggcggg tctggagcgg ttatgccttc gggcggaacg
     2581 tccgggaagg gcagcggccg gcatcgcggt cggcagggac cctgccgttg atggtacact
     2641 ccccctcctt cccgttcttt gcggtataga ggaaacagtc agcgcaggtg ggcatgggag
     2701 aatgattctc cctcccctca cttggagctt tgcctacaaa agagaggaag tgagaaagcc
     2761 gtagaggaat gcgatgccgg caaggcacat cagcgggatt gccaccttgt cagcgatccc
     2821 gaaggtccgc tcccggagcc gggtccgggc acgggtgcgg tatccccgga gatcgatggt
     2881 caggccgagg agggttgccc gcgagagcga gtttgagacg agcgggacca tgaccggagc
     2941 cacgctcctg atccggccca tgaacccgtc gccagagtgg tatccccgtg cgagctgggc
     3001 ttcgtggatc cgtttccctt cgatctggag ggttgggatg aaccgcagcg cgatgatgaa
     3061 catcagggtg tagtcgaccg ggacccttgc tcgttccatg gtgtggacca ggtcgcgggg
     3121 ctgcgtggag atgacaaaga gctggaaggc ggagatcagg atggcgaacc gcagggtcag
     3181 caccgtcccg accgtgaggg caccaatggt gatcgggatg ctgcccccga tgagcgggat
     3241 gaaggagggt ataaggtatc cgatgacagc cccatcctgc accgtcagga tagtgatcag
     3301 gtagatcacg atgctcatga ggaggatgag gaccatctgc tggatgaccg gccggagcag
     3361 gtgccctgca taggcgagca ggaagataag caggaggagc cctgcaagga gggagatgga
     3421 tgaggtgagg atggtcacta cgctcacgat gatgatgaac aggatctttg tgaccgggtg
     3481 gaggcggtga aagatcccgt ctcccggtat gtactggagg atctcagcca tgggctgcct
     3541 ccgccctgac atcatcggct atccggccgg cctccatgtg gatcacccgg tcggcacact
     3601 gccctgcgat ctcgcggctg tgggtgatca tgatgatggt gtgccctttc tcctggagtt
     3661 gtttcaggat ctcgaggatc tcccgcgact ccgacccgtc aaggccggtg gtcggttcat
     3721 ccaggatcag cactgccggc tgcatggcaa gcacgcaggc gatcgccagc cgctgccgct
     3781 ccccccggga gagccagcgg gggtacagat cgcgggcagc ggtaagccgg acggtggcaa
     3841 gggcatccat gaccgcctgc tcccggccgg ggatcccgag gttgtcgaca ccgaacgcga
     3901 cctcatcgaa gacggagtcg gcgaagaaca tgtggtcggg gttctggaag accagcccga
     3961 ccgaatgggc gagctccgcg atcggggctt ttgcagcatc cttcccatcg acggtgaccg
     4021 tccctttgtt ggggcggagc aggccgatga agtgcttgac aagcgtcgtc ttccctgagc
     4081 cattctcgcc gacgattgcc acgaattcac cctgtgcaat cgagaggtcg acgccggaga
     4141 gagcctcctc ctccccgtag ctgaaggtga ggccacgggt gcataccatc ggtgcagagc
     4201 catttacggc tggtgatctg gtcctgatcc ccgaaaaatc ggggatgagg gtccggcgtg
     4261 cgatctcatc ctgcaggaca gttgccgggg gtccttcgtt ccggattcgc ccgttctcga
     4321 gcacaacgat ccgatcgacc agactggcca gtttcgtgaa cttttgctcg acgatcagga
     4381 tagccttgcc ctgtgccttg aactcttcca gaagcctgac aatgctctcg gtcccctgct
     4441 catcgagttc ggacgtcggt tcatccagga tcaggatatc ggtcccgagc gcgagcgtgg
     4501 tcgcaagcac cacccgctgc ttctgcccgc cagacagtgc atggggggga cgatccttga
     4561 gttcttcaag gccggtgacc gcgaggacct gcgcgagccg ctcttcgacc tcggcaacag
     4621 aaaatccgcg ccgttcaatg gccgagagga cctcctcctc gactgtggtg aagaggagct
     4681 gggagtcggg gtcatcaaag aggatgccga cccgggtgga gatatcggtg aggtcatggt
     4741 actcgcgcac gtctttgccg tccacggtca ccctgccggt tttcttcccc ccgtattcat
     4801 gttcgagaat gccggctgcg gccaggcaga gggtggtctt gcctgatccg gagtggccgg
     4861 tgacaaggac gcattcccct ctcctgaggg tgagggtgat ttcagacacc gcttcccggt
     4921 ccgcccgggg ataggcatag ctgacattct cgagcgtgat cattcctgtc cccgcatcag
     4981 gacccggctt gccgggtagt agaggatctg gacgatgacc gcgttgaaga cggcggtgat
     5041 gaccacaatc gggacaaagg caacgacaaa gcccatcatg tcgccattgt atttcgcaag
     5101 gatggttgca ctgacgaaca tgatggctac cgcggcgaag gtgaagccgc tggcgagcgt
     5161 ggtcaggaac gtcgatatgg ttggtgccag ctgggtccgg ttgcgaagca cggtgtaaag
     5221 tccgaagcag accagggctc ccaccggttc actgatcagg tttgccgggg ggaagatcga
     5281 acttgagatc agcatcgaga ggattccggc aacgataccg ataccgagtg cctcgtatac
     5341 ttttggccgg acgaggatga tcgcaaggca gtagaacgca atcaccatgt tgggggtgag
     5401 cggggtgtgg agcatggcca ggaaatagcg caggattgct ccaacggcaa gcaggatgcc
     5461 tactatggca atatctcgtg atttcatggt atttcacctg attttttttt cggagtgagc
     5521 cggggcatgc ccggagatgc agtaaaaaga gtttcagcgc caaggttcga ggacagggaa
     5581 gaatacggtg gatctcatca gagcccgggg aaccgggaat acgataggca tcttgtgatc
     5641 tccttcctcg tgtgatgtat aattttatac aataatgtat aaaaatatac tgataagcaa
     5701 tctccccctt agaggaccag ctgcaggtac acaaaccgca gctggtgccc tcctcaaggc
     5761 ctttctggtt gtcactgcca catgcttatt gctgatggtg agagagaaca gagcatggct
     5821 gaatggaagt actgtctcct ccttctgctg ctgctcggca ttgcagccgg tcccgctgcc
     5881 gcacatccgc cttcggggat gacgctggta tatagcgaac aaaacggcga agtggcaatg
     5941 accgtgaacc atagtgtcag cgatccttcc acccattata tcggtgaagt gaccatcagg
     6001 aagaacggtg ggatggtgat cggagaggtc tattctggac aaccgtccgg ggatactttt
     6061 acgtaccggt atccgatcat cctcactccc ggtgatgaga ttgtcgcaac cgctgaatgc
     6121 aatatcgggg gatcgggaac cgcccggttt cttatgcccg ggcagaccgt cccggctccc
     6181 tcaggaccgg ataccgtccc acggtacgtg taccatgccg ttctgatggt ggctggcatt
     6241 ctctgtatca tcgcttcggg gctgatccct gtctatggga agagaattgc cggctggtac
     6301 cgtctccacg tggtaaccgc tgcgatcggg agcatccttg tcattccggc actcttcctg
     6361 gtcttccggg tctcctacct ttcgttgtct ccctcagcat ttgccatcca cgtgatcctc
     6421 gggctcctcc ttcttctgac cctcattgca acaattgtgc tctccctgat caggaaccgc
     6481 atggggccac ggaagcgtct tttcaggaat gcccatatct ggatgggccg agtgttcatc
     6541 atcctcatgg tggtgaatgt gctgaccggg cttgcggcgg taggggttat ctaatttttt
     6601 ccggaatttt tggtccagac agtaggggac ttcatcttgg ttatttttaa tgttacatga
     6661 tttttggtcc tcctccaccc catctcatga taccgaacgt acctttgctg cgatggcttc
     6721 aaaagttgct gagggatctc tggaatagct accttcctga agagagagat gagagcgggg
     6781 gaagacgttt ggtcctgcaa aaaatgttgt ttatggtctg atgaaaaccc cgtccttagt
     6841 ccctccccag ggttctcatt cccaggtgat
//
LOCUS       DAOOCO010000100         6763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10547_length_6763_cov_5.064252, whole genome shotgun sequence.
ACCESSION   DAOOCO010000100 DAOOCO010000000
VERSION     DAOOCO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10547_length_6763_cov_5.064252"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /gene="infB"
                     /locus_tag="PK154_06555"
     CDS             <1..1415
                     /gene="infB"
                     /locus_tag="PK154_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW10759.1"
                     /translation="RTPFVVAATKIDRIHGWRANTDQPFLASFSHQNDRVKEIVETRL
                     YEMVARLSDLGFSCERFDRVSDFQRNLAIVPVSAHTGEGIAELLMVMIGLAQRYMEQE
                     LALSVEGPGMGTVLEVKEERGLGTTLDVILYDGTLSIGDEIAFARNDGVAQTKVRSLL
                     KPRPMKEILIEDRFERVKTVVAATGIKVSAPGLDGTIAGSPLKVIRGDPGPVMEQIQR
                     EMEEIKVKLSPEGLVIKADTIGALEAICNELETKEIGVMRAEVGPVSRHDLIEVETIK
                     SPYYRVLLAFNTQILPDAADMIKDPGFSHVKVFEGKVIYKIVEEYVLWKDEQKRRAEQ
                     QKFEQIITPAKIRVLPHCVFRQSNPAVVGVRILGGTLRSDVNLIHVDGRKVGHLKSIQ
                     LNQESLREAQAGAEVAISIEGATYGRQFSIEDDLFVDIPERHVKVLEREMSSHLPIST
                     QEILGEFTTLKRRSDPFWGK"
     gene            1563..1967
                     /locus_tag="PK154_06560"
     CDS             1563..1967
                     /locus_tag="PK154_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HPW10760.1"
                     /translation="MVEFKVVLSDQKTGRAYNVNVSGGVAGALIGKRIGDEVDAGPFG
                     LPGYRMEITGGSDRNGTPSKRNLPVSGRRRLLLSGGIGFKPVMEGQRRRKSIRGNEIT
                     ADFVQINAKVTGYGEKPLEEYFTKPAPEEKKA"
     gene            complement(1968..2432)
                     /locus_tag="PK154_06565"
     CDS             complement(1968..2432)
                     /locus_tag="PK154_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HPW10761.1"
                     /translation="MSLAITIAAPFRHTRKELLKKNEIVYYLAFERGWMNIEQANHLL
                     ARAKEDGLISYEGDMIRPLFDVISMEIPLGFKPNASVFHRSDPHEELMSRIARDLSIP
                     LTRVVAEMNTIISKEFGGHIRPEGAVVILAKRSGVEFSDLLPALRENILKSD"
     gene            2597..2923
                     /locus_tag="PK154_06570"
     CDS             2597..2923
                     /locus_tag="PK154_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10762.1"
                     /translation="MTNPKEYAEKTKAHIDQLADEIEKLQAKTDKVMEELRAEYDRKL
                     REISYEYDRKLQELRGSEQDLGRKIENIKIAGGGAFEEMKAGADLAVADMKNAVARAK
                     EKFRDI"
     gene            complement(3070..3447)
                     /locus_tag="PK154_06575"
     CDS             complement(3070..3447)
                     /locus_tag="PK154_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HPW10763.1"
                     /translation="MQWQGRSIRRLTGGRYRPSRGKKRNEIGSAPTDTHIGEDRRRIV
                     RTFGGSPKVRAMRAKYANVANRSNGEIKKVLIETVEENGANPNYVRRNLLTKGAVIRT
                     EMGRARIISRPGQDGVINAVLLE"
     gene            complement(3553..4200)
                     /gene="hypB"
                     /locus_tag="PK154_06580"
     CDS             complement(3553..4200)
                     /gene="hypB"
                     /locus_tag="PK154_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPW10764.1"
                     /translation="MHHIDVRLEKDIFDVNNRIAEKNAACFKAHGIRAIDLLGAIGSG
                     KTALIERIVPLLSERGLKSAAIAGDVFGDDDYKRIKALGIPVENANTGKECHLDAHLV
                     EHASEHLPLDLIDVLFIENVGNMVCPTDFLLGAEIRVVIVSSTEGDDVVNKHPMMFRG
                     CDIGIINKVDLAPLVGADLNRMEADMHRYNPGMQVFRTNLKTGEGLSDVVAAFLG"
     gene            4268..4540
                     /locus_tag="PK154_06585"
     CDS             4268..4540
                     /locus_tag="PK154_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HPW10765.1"
                     /translation="MKGERILYPCYFNANLMRGEGRRITRSRAAKDPSLSDIERAVKR
                     CGIQYRIEQKSHPSYWWKREGRIVVAWAQSKEQLLKKVAGFLEVKR"
     gene            4537..5190
                     /locus_tag="PK154_06590"
     CDS             4537..5190
                     /locus_tag="PK154_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPW10766.1"
                     /translation="MSGLYDLHTHTTLTDGEMIPIELVRRMSVLGYSIVAITDHADNT
                     NLDFLIESVNRLKTPAELYGVRLLCGVELTHIPPPLIPCTARAAKKNGADIVIVHGES
                     PVEPVASGTNHAACTCSDVDVLAHPGFITTKDAYEAHDRGIALEITSRGGHNRANGHV
                     AAVARKAECIMVVNSDAHAPHDLLDKRAKFDVARGAGLKKEECAPIMDLNIDCIVFR"
     gene            5303..5530
                     /locus_tag="PK154_06595"
     CDS             5303..5530
                     /locus_tag="PK154_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HPW10767.1"
                     /translation="MKAIGTAMHTLGTRIIVMRCDPAQLPKLYADVISKRLKPVGKVV
                     EVFGSVAAPYAAVHCHSDYRIAAGEKLFISR"
     gene            5542..6552
                     /locus_tag="PK154_06600"
     CDS             5542..6552
                     /locus_tag="PK154_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HPW10768.1"
                     /translation="MQEVEKLKILQNEREALKNRLKERVKEHEKKAEDHTETVCPECG
                     GRQLVHDYERAELVCQNCGLVIDEDFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHDK
                     GLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMAS
                     ALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCNVPRTLDEIAEVS
                     RVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGER
                     ELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIEI
                     IL"
     gene            6690..6761
                     /locus_tag="PK154_06605"
     tRNA            6690..6761
                     /locus_tag="PK154_06605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6722..6724,aa:Ala,seq:cgc)
BASE COUNT         1738 a         1629 c         1769 g         1627 t
ORIGIN      
        1 gcaggacccc gtttgtcgtt gctgccacaa agatagacag gattcacggg tggcgggcaa
       61 atactgatca gcccttcctg gcatcatttt cccaccagaa tgaccgtgtg aaagagatcg
      121 ttgagacccg gctttatgaa atggtagccc ggctttcaga cctcggtttt tcttgtgagc
      181 ggtttgacag agtctctgat ttccaacgga acctggcaat cgtgccggtc agtgcacata
      241 cgggagaagg gattgcggaa ctgctcatgg ttatgatcgg tcttgcacag cggtatatgg
      301 aacaggagct tgccctctcg gttgaagggc ctggcatggg cacggtcctt gaagtgaaag
      361 aagagcgcgg gctcgggaca accctcgatg tcatccttta cgatggcaca ctctctatcg
      421 gcgatgagat cgcctttgcg cggaatgacg gggttgcaca gacaaaggta cgatcgcttt
      481 taaaaccacg cccgatgaaa gagatcctca tagaagatcg gtttgaacga gtgaagaccg
      541 ttgttgcggc aacaggaatc aaagtgagtg ccccagggct ggacggtacc attgcggggt
      601 ctccgctcaa ggtgatacgg ggagatcccg ggccggtcat ggaacaaata caaagagaga
      661 tggaagagat aaaagtcaag ctctcaccgg aaggactggt gataaaggct gataccatag
      721 gtgcccttga agcgatatgt aacgagctcg aaaccaagga gataggagtg atgcgggcag
      781 aggtgggccc ggtgagccgc catgatctta tcgaggtaga aacgatcaag agtccgtatt
      841 accgcgtcct tctcgctttt aacacgcaga ttcttcccga tgctgctgat atgatcaagg
      901 atcccggatt ctctcatgtc aaggtctttg aaggcaaggt catttacaag atagtcgaag
      961 aatacgtctt atggaaggat gagcagaaga gaagggctga acagcagaaa ttcgaacaga
     1021 ttatcactcc cgcaaagatt cgggttctcc ctcactgcgt cttccggcaa agcaatcctg
     1081 cagtggtagg tgtccggata ctgggaggca ccctcaggag cgatgtgaac ctaatccatg
     1141 tggacgggag gaaggtcggc cacctcaagt ctattcagct gaaccaggag agtctcaggg
     1201 aagcccaggc aggcgccgag gtcgccatct caattgaagg tgcgacatac ggaaggcaat
     1261 tttccattga agatgatctc tttgttgaca ttccagaacg acatgtaaag gtccttgaac
     1321 gggagatgtc ttcccatctc cccattagca cacaggagat ccttggagag tttactactc
     1381 tgaaacgtcg ctcggatcct ttctggggta aatagtattt aatagatcag gcacccaaaa
     1441 taatgggtac ttcccggagt ttcccgcaga caggctattt gcgtatcctt cgttaatgac
     1501 gcatatggca tccagcgggg ttctcaggtt ccggaataat aatatcgagg agtctagacc
     1561 aaatggttga attcaaggtt gtattgtctg atcagaagac tggacgcgcc tataatgtca
     1621 atgtgagcgg tggagtcgca ggggcactta tcggtaagag aattggtgac gaggttgatg
     1681 caggaccttt tggtctcccc gggtatagga tggagatcac aggcggttct gacaggaatg
     1741 gtactccctc aaagcggaac ctgcctgttt cagggaggcg gcggctcctc ctctcgggcg
     1801 ggattggttt caagccggta atggaaggtc agaggcgaag gaaatctatc agaggcaatg
     1861 agataaccgc tgattttgtg cagatcaatg caaaagtcac tgggtatggt gaaaaacctc
     1921 tcgaagaata ctttaccaag ccggctcctg aagagaagaa agcataatca atcacttttc
     1981 agaatatttt cccgtagtgc ggggaggaga tctgagaact caacaccact tcgcttcgca
     2041 aggatgacaa cagctccttc tggtcttata tgacctccaa attccttact gataatagtg
     2101 ttcatctccg caacaaccct tgtaaggggg atgctcaggt cccgggcgat tcgtgacatc
     2161 aactcctcat ggggatcaga ccggtggaag actgatgcat tgggtttgaa accaagcgga
     2221 atctccatcg atataacgtc gaaaagcggc cgaatcatat ccccttcgta cgatatcagg
     2281 ccatcctctt tcgccctcgc aagaagatgg ttggcctgtt cgatgttcat ccatccccgt
     2341 tcaaaagcaa ggtagtaaac aatttcattt tttttcaaca gctccttcct tgtgtgcctg
     2401 aacggagcag cgatagtaat ggccagactc atatcacgac ttcccttttt ccctgtaaag
     2461 gggatgatct ctcataggtc ccacaactcc acaaaaagta tcttcattct ggtccgtatc
     2521 aatcaatcgg tccgcatatt caatctggga tcctttctca caaagaaata attgatgtca
     2581 atataaattc tggagtatga caaatccaaa ggaatacgct gaaaaaacga aagctcacat
     2641 tgaccagctt gctgacgaga tcgagaaact acaagcgaaa accgacaagg ttatggaaga
     2701 gcttcgtgct gaatacgatc ggaaattacg cgaaatctcg tatgagtatg atcggaaact
     2761 gcaggaactg agggggagcg aacaggatct gggcaggaaa attgaaaaca tcaaaatcgc
     2821 cgggggagga gcattcgagg agatgaaagc aggtgctgat ctggcagttg ctgatatgaa
     2881 gaatgccgtt gcacgggcca aggaaaaatt cagggacatc tgaccaggcc aacatctttt
     2941 ttggatattc taggtcatga ctaccatggt agttttccct gttcaaaaac gaagccattt
     3001 cacgcttttt cgtgaaaaat ggattggatg gggggtgcaa ttttccgccc cccattgtaa
     3061 ctacggaaat tattcaagga ggacggcgtt gataactccg tcctggccag gcctgctgat
     3121 gatgcgggca cgccccatct cggtcctgat cacggcaccc tttgtgagaa gattacgccg
     3181 tacatagttg ggatttgcac cgttttcctc aactgtttca ataaggactt ttttgatctc
     3241 tccatttgac ctgtttgcta cattagcata tttggcccgc attgccctga ctttcgggct
     3301 gcccccgaat gtccggacaa tccttctcct gtcttccccg atatgggtgt cggtcggtgc
     3361 tgaccctatc tcatttctct tcttgccgcg ggaaggtcga tatctccccc cggtcagcct
     3421 gcgaattgat ctgccctgcc actgcattgt ttcatccctc tgtcagaact ctgaattatc
     3481 agcatccggg agaatcaccc ggacaagtgc tatacatata cccttgcatg ttatttaatt
     3541 gctctgcttg gttcatccaa ggaaagcggc aaccacatcc gacaaacctt ctcctgtttt
     3601 gagattggtg cggaacacct gcataccggg attgtaccgg tgcatgtcag cctccatgcg
     3661 attgagatcg gctcctacaa ggggagcaag atccactttg ttgataatgc caatgtcaca
     3721 gcccctgaac atcatagggt gcttgttcac cacatcgtca ccctcagtag agcttacgat
     3781 aacaacccgg atttctgctc cgagcagaaa atcggtgggg cagaccatgt tacccacgtt
     3841 ttcgatgaaa aggacatcga tcaggtcaag agggagatgt tcggaagcat gttccacgag
     3901 gtgagcatca agatggcact ctttgccagt attggcgttc tcaaccggga ttccaagagc
     3961 tttgatcctt ttatagtcat catctccgaa tacatcacct gcaatcgccg cgctcttcag
     4021 cccccgttca gagagcaaag ggactatccg ttcgatgagg gcggtctttc ctgacccgat
     4081 agcccccaaa agatcgattg cacgaatccc atgcgccttg aaacaggctg catttttttc
     4141 agctattcgg ttattgacgt cgaatatgtc cttttccagt ctgacgtcga tatggtgcat
     4201 gtatcaaata tggatgctgc agtgtttata ggttccatca gctcacatta ctacaatgaa
     4261 agaggagatg aaaggagaac ggattctcta tccctgttat ttcaatgcaa acctgatgag
     4321 aggcgaggga aggcggatca cccgctcccg ggcggcaaag gatccctccc tttcggatat
     4381 cgaacgtgcc gtgaagcgat gtggtatcca gtacaggata gaacagaaaa gccatccgtc
     4441 ttactggtgg aaacgggaag gtcgcattgt ggtcgcgtgg gcacaaagta aggagcaact
     4501 gctgaaaaaa gtcgctggtt ttctcgaggt gaaacgatga gcgggctcta tgatctgcat
     4561 acccatacca cgctcactga tggtgagatg ataccgatag agctggtgcg acggatgtct
     4621 gtccttggtt attccatcgt tgctatcact gatcatgctg acaacaccaa ccttgacttc
     4681 ctcatcgaat cggtcaatcg tctgaaaacc cctgcagaac tgtatggggt ccggcttctc
     4741 tgcggagtcg agcttacgca tattcctccc cctcttatcc cttgtactgc cagggctgcg
     4801 aaaaaaaacg gcgctgatat tgttattgtc cacggggagt ctccagttga accagttgca
     4861 tccgggacca atcatgccgc gtgcacctgc agcgacgttg acgtgctcgc tcacccgggt
     4921 tttatcacga ccaaggatgc ctatgaggca catgaccggg ggattgccct tgaaatcacc
     4981 tcaaggggag gacataaccg tgcaaatggt cacgtggccg cggttgccag gaaggcagaa
     5041 tgcatcatgg tcgtgaactc agacgcgcat gcaccccatg atctccttga taagcgcgcg
     5101 aaatttgacg tagcaagagg tgcaggtctt aaaaaggaag agtgcgcccc cattatggat
     5161 ttaaatattg attgtatcgt tttccgttga aattggtatt tataccttta caatacttta
     5221 atatagtggg aaagtctata tatatagatt acgaaaccat ttcacctgtc tgatatccgg
     5281 ggcaggtgca cggagtttta tcttgaaagc gattgggact gcgatgcata cactagggac
     5341 gcgcatcatt gtcatgaggt gcgatccagc ccagctccca aagttatacg cagatgttat
     5401 cagcaagaga ttgaaacccg tcgggaaagt ggtggaggta ttcgggagtg tagccgcacc
     5461 ttatgcagca gtccattgcc atagtgacta ccgaattgct gcaggggaga agctgttcat
     5521 atccaggtag gagtcatcac catgcaggaa gttgagaaat taaaaattct ccagaatgag
     5581 agggaagcgc tcaaaaaccg gctcaaagaa cgcgtcaagg agcatgaaaa aaaggctgaa
     5641 gaccataccg agaccgtttg cccggagtgc ggaggccgac agcttgtcca tgactatgaa
     5701 cgtgccgagc tggtctgcca gaactgtggt cttgtcatcg atgaggattt cattgaccgt
     5761 ggcccggaat ggcgtgcatt tgaccatgat cagaggatga aacgctcgag ggtcggagct
     5821 cctatgacct tcaccatcca tgacaagggg ctttccacga tgatagactg gaggaaccgg
     5881 gactcgtatg gacgggccat ctccagcaag aaccgtgccc agctctaccg actgagaaaa
     5941 tggcagcgcc gtatccgtgt cagcaacgcc actgaaagga acctggcatt cgcgctttct
     6001 gagcttgacc gtatggcctc tgcccttggt ctgccacgta atgtccggga gaccgcagcg
     6061 gtggtctatc gtgacgctgt tgacaagaac ctcatccgtg gaaggagcat tgaaggagtg
     6121 gctgctgcag cactgtatgc agcctgcagg cagtgcaatg ttccaaggac tcttgatgag
     6181 attgccgagg tatcccgggt atcacgaaaa gagatcgggc ggacgtaccg gttcatatcc
     6241 agggagcttg ggctcaaact ccttccaaca tctccgatcg attatgtccc gcggttctgc
     6301 tccggcctta acctgaaagg agaagttcag agccgggcgg tggagatcct ccgccaggct
     6361 ggagaacggg agctcaccag tgggaggggg cccacgggtg ttgccgctgc ggcaatttat
     6421 atctcatcca ttctcggcgg tgaacggaga acccagcgtg aggtagcgga agttgccggc
     6481 gtgactgaag tcaccatccg taacagatac aaggaacttg ctgagaaact cgatatcgag
     6541 attattctct gaacctcctt tttctctgtt ctttaccata gttattccga atgagttcct
     6601 ccgtcccgtc gcaaaaggta ttattgatgt atttcatcgc ggaaccaaac aactatatcc
     6661 aaatgactga aaaattagtt accgcataag ggcttgtaga tcaggggtag atcgttacgt
     6721 tcgcaacgta aaggccgcgg gttcgaatcc cgccaagtcc atg
//
LOCUS       DAOOCO010000101         6731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10618_length_6731_cov_4.818754, whole genome shotgun sequence.
ACCESSION   DAOOCO010000101 DAOOCO010000000
VERSION     DAOOCO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_6731_cov_4.818754"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK154_06610"
     CDS             <1..316
                     /locus_tag="PK154_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10769.1"
                     /translation="AQIPFSDELPRTDLALNLPALMTLIPGQFSLSYSRLILGGRTLA
                     SLTFDGEFDCHFISGLRDLFSGDAGVSPCQILGERDMLTAPDVVRAMLAWRIIQDIIS
                     EQ"
     gene            380..1837
                     /locus_tag="PK154_06615"
     CDS             380..1837
                     /locus_tag="PK154_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW10770.1"
                     /translation="MGRMRYFSTVFAELGGMLEYIGPMTAVPLLVAVAFREYSLLLPM
                     AAVPATLFALGFLLNRLPRRKEDIRLSSAFCSVALVWLVFALVSTIPFILVLDLSLTD
                     ALFETMAGWTGTGFSLLHPSGHIPETLLFWRTFMQWIGGIGVIALSITMAGRGGLVQS
                     PLFRSDSRSERILPGVIATGKEIWAVYVFLTLIGAGVILIAGVPLYEAIMLSLTTIST
                     GGYLPFEGGLAIYESRLLEYLLIPVMIVGATPFTLYYISYRKRKLSLFGNEQVKLLLL
                     FLAAGSAVVIADLYYLNSLPLTEAVRQGLFFTAAAISTTGYQGQQIQLFPSVTIVFLM
                     LLMFIGGSSESSAGGVKLSRIAIGYRGILWWFKRVFVRTKVLVPFMYGGVKVPESVAE
                     PELSKNMLVIILSVLTVFIATMVVLQFHLISLDVTNLVFDIVSALSSCGLSAGYVTPG
                     MPFVSKWVFIIVMWIGRLEVIPVIVLLLGIFRAGD"
     gene            1904..2722
                     /gene="cfbC"
                     /locus_tag="PK154_06620"
     CDS             1904..2722
                     /gene="cfbC"
                     /locus_tag="PK154_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HPW10771.1"
                     /translation="MKQIALYGKGGIGKSTTSTNISAALSGMGLDVLQIGCDPKRDST
                     RMLMHGRLIPTVMDQVRERGDKAITQEDVVFTGYGGVRCVEAGGPEPGVGCAGRGIIA
                     TFQVLERLGALHADVVVYDVLGDVVCGGFAMPMREGYAQEVYLVTSGELMSLYAANNI
                     CKAIRRLSGRSRGQCRLGGVICNAKNLPDEKQLVATFAERIGSTLIQFIPRDPVVQRA
                     ELNRMTVIEYLPGSDQAEAYRLLARNIIENRRLDIPEPLEIAELENLAYDFIAD"
     gene            2703..3716
                     /locus_tag="PK154_06625"
     CDS             2703..3716
                     /locus_tag="PK154_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW10772.1"
                     /translation="MISSRTEGCTLTGALSVTTSVQDAITVIHGPDGCAHHNFSLLHA
                     LHFDNDQLTLPSILSSSLTEQEIIFGGEIALEETLSGAMGESPGAIFVLTSCVAAAIG
                     DDVRAVCNEIRGCPVVVIPTGGFLGGGFSDGVRSALIALSELGKGGDPNGTVTLVGEK
                     NLEYEAEANYREVQRLLGLLGVKIRLRFVRNIAVSEIAQLGSSSLNILREESLADVGD
                     LLNDRFGTPYLPSFPLGFEGTLTFLSQVGHALNTGSDRAISMELERQKGIIDQFSELR
                     GRAVNICHTDHMPAPLLSEVLELFDLQVKERGQTLTLPDPIPVGTSGLSRMLSRWQRV
                     LHA"
     gene            3709..4839
                     /locus_tag="PK154_06630"
     CDS             3709..4839
                     /locus_tag="PK154_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW10773.1"
                     /translation="MRDCRNALWPCAMTGAVTCFAGIRGIGTIIHGPSGCYFFPATVL
                     HRRLYCTFLIEEDIIFGAEARLLELVERLREEYEAVAVVNTCTPAITGEEIRTCLPGE
                     ILMVDSPGFLGNFEEGYHAACRTLPARTDPETAGVNLDGLQLLDPFCAGNALEAQRIL
                     AAAGVGVAARFCDETLGGMRHSSGYSVHTNPDLSSGFGRSMGTLLGIAETGETLKRLE
                     KTLEGVDLEAFEYDMESVGSAIETSCEKFLMRHDPPVVAIFSGFSYADFAARMLKHYL
                     DASVTILGLRNAPGNSRFRTAEACSLSAVQDLIAADPPDLLLGSSFERMACPDAAFVP
                     FTYPLRGMVRLKARPLVGIQGIAGLMEDVLNACLDHQDSLNT"
     gene            4989..5432
                     /locus_tag="PK154_06635"
     CDS             4989..5432
                     /locus_tag="PK154_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10774.1"
                     /translation="MMKNAGYLQLNRRPVLDEEGIPQDILELYLYGTRLCIRINELRK
                     ALRSGLSVSVERIQRNWMAYIGRSAGNARISKSGKALNIELSNGERFTVSLDSLHAVL
                     CSRERYASVAVLPANITGQIVQNRKITDYCPLSAREGATRKALPA"
     gene            complement(5455..6570)
                     /locus_tag="PK154_06640"
     CDS             complement(5455..6570)
                     /locus_tag="PK154_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPW10775.1"
                     /translation="MYRVAAIGGDGIGPEIIAEGRKVLDAAAERYGFDLGWDEFDIGA
                     DRYLKSGELVTEEDQKELSRYRAIYFGAIGDERVPPGILEKGILLHLRFCLDLYVNLR
                     PIRLLSGVPTPLAGKGPSDIDFMVVRENTEDFYVGIGSRFHGREKKTLTIERELYQAR
                     FNLDIESDADELAYQVGVITREGAERVISYAFDLARQRRKKLTSVDKANVLSDVYGLW
                     REVFSEVAARNPGISTEFTFVDAVTMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGG
                     LGLAPGANINPGGTSMFEPIHGSAPKYKGLNVANPIATVWAGSMLLDHLGEHEAAAAV
                     LKAIERSIADGVVTRDMGGTATTSSVGDHLADILLKG"
     gene            complement(6549..>6731)
                     /locus_tag="PK154_06645"
     CDS             complement(6549..>6731)
                     /locus_tag="PK154_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPW10776.1"
                     /translation="AIPDGDECRVDIDKHLITCGERTYPIEPVPGFLLRIADAGGLIP
                     FARNLEEVDRCTGSQQ"
BASE COUNT         1394 a         1927 c         1800 g         1610 t
ORIGIN      
        1 cgcccagatt ccgttcagcg atgaacttcc gaggacggat ctggcgctga atcttcctgc
       61 cctgatgacc ctcattccag ggcagttctc tctctcctac tcccgtctga tcctcggcgg
      121 ccggaccctg gcctcgctca cgttcgatgg cgaattcgat tgccatttca tctcgggcct
      181 ccgcgatctc ttttcagggg acgcaggggt aagcccctgc cagatcctgg gcgaacggga
      241 tatgctcacc gctccggatg ttgttcgggc aatgctcgcc tggagaatta tccaggatat
      301 tatttcagag caataaaaag gagagaaccg gctcaccacg caaaaaagat aatctcctta
      361 caacaatcat tgtcttacaa tgggccgcat gcggtatttt tccaccgtgt ttgccgaact
      421 tgggggaatg ctcgaataca tcggcccgat gactgcagtt ccccttcttg ttgcggtcgc
      481 attcagggag tattcactcc tccttcccat ggctgccgtt cctgcaaccc tttttgcact
      541 gggcttcctt ctgaaccgtc ttccacggag aaaagaggat atcaggctct cctccgcctt
      601 ctgttctgtg gcactggtct ggctcgtttt tgcgcttgtc agcaccatcc cttttatcct
      661 tgtgcttgac ctgtccctta ccgatgccct tttcgagacc atggccggat ggacaggtac
      721 cggattttcc cttctgcacc cgtccggtca tatccctgaa acattgctgt tctggcggac
      781 attcatgcaa tggatcggag ggattggggt tattgccctc tccatcacca tggcaggccg
      841 tggaggtctg gtgcaatcac cgctcttcag gtccgacagc aggagtgaac ggatccttcc
      901 tggggtgatt gcaacaggaa aggagatctg ggcagtctat gtcttcctta ccctcatcgg
      961 tgcaggggtg atactgattg cgggagttcc cctctacgag gcgatcatgc tctcgctgac
     1021 taccatctcc accggaggct atctcccttt tgaggggggt cttgccatat atgagagcag
     1081 gctcctcgaa tatcttctca tcccggtgat gattgtcggt gcaacaccct ttaccctcta
     1141 ttatatctcc taccggaaac ggaaactctc cctctttggt aacgagcagg tcaaactgct
     1201 cctcctcttc cttgcggcag gctcagccgt ggttatcgcg gatctgtact atcttaacag
     1261 cctcccgctc actgaggccg tgaggcaggg cctctttttc accgcagcgg ctatcagcac
     1321 gacgggatat caaggccagc agatccagtt gtttcccagt gtcaccatcg tattccttat
     1381 gctcctgatg ttcatcggtg gctcttccga gagcagtgcc ggcggggtca agctctcccg
     1441 cattgcgatc ggataccggg gaatactctg gtggttcaaa cgggtctttg tccggacaaa
     1501 ggttctggtc cccttcatgt atgggggagt taaagtcccc gagagcgtgg cagagccgga
     1561 attgtccaag aacatgctgg tgatcatcct gtcggtgctg accgtattca ttgcaacgat
     1621 ggtggtactc cagttccatc ttatctctct tgatgtgacc aatctggtct ttgacatcgt
     1681 ctctgccctg agctcctgcg gactttcggc tgggtatgtc acgcccggga tgccgtttgt
     1741 atcaaaatgg gtcttcatca tcgtcatgtg gatcggacgg ctcgaagtta tcccggtgat
     1801 tgtgctcctc cttgggatct tccgtgctgg cgattagccg gactcaagta aactggtgta
     1861 aacttcagaa tagctaatat aaaataccga cttagatctg ataatgaaac agatcgccct
     1921 gtacggaaaa gggggtatcg gaaaatccac cacttccacc aacatatcag cagccctttc
     1981 cgggatgggg ctcgatgtcc tccagattgg atgcgatccg aaacgtgaca gtacccggat
     2041 gttgatgcac ggcaggctga tccccacggt catggaccag gtccgggaac ggggcgacaa
     2101 ggcaattacg caagaagacg tggtatttac cgggtacggg ggtgtccggt gtgttgaggc
     2161 aggtggccca gaacccgggg ttggctgtgc cggcaggggg atcatcgcca cctttcaggt
     2221 gctcgaaagg cttggagccc tccatgcgga tgtggtcgtg tacgatgtgc ttggtgatgt
     2281 ggtctgcggt ggctttgcta tgccgatgag ggaagggtat gcacaggaag tatacctcgt
     2341 tacttcaggg gaactgatgt ccctgtatgc tgccaataat atctgcaaag ccatccggcg
     2401 gctatccggg cgttcacgcg gccagtgccg gctcggcgga gtgatctgca atgccaagaa
     2461 cctccccgat gagaagcaac tggtcgccac ctttgcggaa aggataggaa gcactcttat
     2521 ccagtttatt ccaagggacc cggtcgttca gcgtgctgag ctcaaccgca tgaccgtcat
     2581 cgagtatctt cccgggtctg accaggcaga ggcataccgc ctccttgccc gaaacatcat
     2641 cgagaaccgg cggctcgata ttcccgaacc tcttgagatc gcagagctcg agaatctggc
     2701 atatgatttc atcgcggact gaaggatgca ccctgacagg tgcactttct gttaccacat
     2761 cggtccagga tgccatcacg gtcatccatg gccccgacgg atgtgcccat cacaattttt
     2821 cccttctcca tgccctccat ttcgataacg accaactgac cctgccatcc atcctctcga
     2881 gctcgctgac ggagcaggag atcattttcg gtggtgagat tgctctcgaa gaaactctct
     2941 cgggagccat gggcgaaagc ccgggcgcca ttttcgtgct gacctcctgt gtcgctgcag
     3001 cgatagggga cgatgtcagg gcggtctgca acgagatcag gggatgcccc gttgtcgtca
     3061 tccccaccgg aggattcctc gggggtggct tttctgacgg tgtgagatca gcccttattg
     3121 ccctctctga actggggaaa ggaggagatc ccaatggaac cgtaaccctt gtcggcgaga
     3181 agaaccttga gtatgaggca gaggcaaact accgtgaagt gcagcgcctt ctgggattgc
     3241 tcggtgtgaa gatccgtctc cgttttgtca ggaacattgc cgtttccgag attgcgcaac
     3301 tcgggtcttc ctcgctcaat atcctccggg aagaatctct tgctgatgtc ggcgatctgc
     3361 tcaatgaccg gttcggtacc ccttatctac cctcgtttcc tcttggattc gaggggacac
     3421 tcacgtttct gtcccaggtg ggtcacgcgt tgaacaccgg gagtgaccgg gcaatctcaa
     3481 tggaactgga gcgccagaag gggatcatag accaattttc agaattgaga ggaagagcgg
     3541 tcaacatctg ccataccgac catatgcccg ctccgctcct tagcgaggtg ctggaacttt
     3601 ttgaccttca ggtaaaagaa cggggacaga ccctcaccct gcccgacccg atcccggtcg
     3661 ggacttcagg actttcccgt atgctcagcc ggtggcagag ggtactccat gcgtgactgc
     3721 cggaacgccc tgtggccttg cgcaatgaca ggagcggtca cctgctttgc agggatccgg
     3781 gggatcggga ccatcatcca tggaccgagc ggatgctact tctttcccgc gacagtcctt
     3841 caccggaggc tttactgcac cttcctgatc gaggaagata tcatctttgg tgccgaggca
     3901 cggctccttg agctcgtgga gaggctacgg gaagagtacg aagcagtcgc tgtggtgaat
     3961 acctgtactc ctgctatcac cggcgaggag atacggacct gcctgccggg agagatcctt
     4021 atggtcgaca gtcccggttt tctcggaaat tttgaagaag gataccatgc cgcgtgcagg
     4081 accctccctg ccaggaccga tcccgaaacc gccggggtga accttgacgg gttgcagctc
     4141 cttgacccgt tctgtgcggg aaatgctctt gaagcacagc ggatcctcgc tgcagccggt
     4201 gttggtgtcg ccgcccggtt ctgtgatgaa acccttggcg ggatgaggca ttcttcaggg
     4261 tattccgtcc atacaaatcc cgatctctct tccgggtttg gccggagcat gggaactctt
     4321 cttgggatag ccgaaaccgg ggagacgctc aaacgccttg agaaaactct tgagggcgtt
     4381 gacctggaag cgtttgagta cgacatggag agtgtcggga gtgcaataga aacctcctgt
     4441 gagaaattcc tgatgcggca tgatccgccc gttgtggcca tcttctcggg cttctcctat
     4501 gccgattttg ccgctcggat gctgaaacac taccttgacg cttccgttac cattctcggt
     4561 ctccgcaatg ctcctggtaa ttcacgattc agaaccgcgg aagcctgctc tctctcagcc
     4621 gtgcaggatc ttattgccgc agacccgccc gatctcttgc tgggttcttc ctttgaacgt
     4681 atggcatgtc ccgatgccgc tttcgtcccg ttcacctatc ccctgagggg catggtcagg
     4741 ctgaaagcac ggcctctcgt cgggattcag gggattgcag ggttgatgga ggatgttctc
     4801 aatgcctgcc tcgatcacca ggattccctg aatacctgaa aagaatcttt ttatggtatt
     4861 ttgcaggcac agactgtttt cccagtcatg caaccctgac tactcccggc cggacacttt
     4921 cgcactgcac agattgaacg tttatatagt catggtctcg acgtaactgt caggaagagt
     4981 caaaaatcat gatgaagaac gcaggatacc tgcagttaaa ccggcggcct gtccttgatg
     5041 aggagggaat accgcaggat atcctcgagc tgtaccttta cggcacaagg ctctgcatcc
     5101 ggatcaatga actccggaaa gccctccgtt ccggcctttc cgtgagtgta gagagaatcc
     5161 agcggaactg gatggcgtat atcggaagga gtgcaggcaa cgcccggata tcaaaatcgg
     5221 ggaaggccct caacatcgag ctttcgaatg gcgaacgatt caccgtttcg ctcgattcgc
     5281 tccatgccgt gctctgctcc cgggagcggt atgcctcagt tgcagtgctc ccggcaaata
     5341 tcacaggaca gatcgtgcaa aacaggaaga tcaccgatta ctgcccgctt tctgcacggg
     5401 agggtgcgac caggaaagct ctcccggcct aatcccccca gcctcgtgtc tgcctcagcc
     5461 tttaagaagg atatccgcga ggtgatcgcc aacagaggag gtggttgcag tgccacccat
     5521 gtccctggta acaactccgt cagcaatcga tcgctcgatc gccttcagca ccgctgcagc
     5581 cgcttcatgc tcacccagat gatcaagcag catcgaacct gcccagaccg ttgcgattgg
     5641 gttggcaaca tttagtcctt tgtattttgg tgccgatcca tggatcggct cgaacatcga
     5701 ggtccctccc ggattgatat tggcacctgg tgcaaggccg aggccgccct gtatcatggc
     5761 ccccaggtcg gtgatgatat ccccaaacat gttcggcgtg accactacat cgaaccactc
     5821 gggattcttc acaaaccaca tggtgaccgc atcaacaaag gtgaattcgg tcgagattcc
     5881 cggattcctg gcagcaactt ctgaaaatac ctccctccag agcccataca catcggagag
     5941 gacattggcc ttgtcaacgg aggtcagctt ctttcttcgt tgtcgggcaa ggtcaaaggc
     6001 ataggatatc acccgttcag ccccttcccg ggtgatcacc cccacctgat aggcaagctc
     6061 atcggcatcg gattcgatgt cgaggttgaa ccttgcctga taaagctcac gctcaatggt
     6121 aagtgtcttt ttctctctcc cgtgaaaacg ggatccgata ccgacgtaaa agtcctcagt
     6181 gttctccctt acgaccatga aatcgatgtc ggaagggccc ttccctgcaa ggggtgtcgg
     6241 cacccctgaa agcagcctta tcggcctcag atttacgtag aggtcgaggc agaaccggag
     6301 gtgaaggagg atcccctttt cgaggatccc cgggggtaca cgttcgtccc ctatcgcacc
     6361 aaagtagatg gctcggtacc gggagagctc cttttgatcc tcctcggtga caagctcccc
     6421 gcttttgaga taccggtccg caccgatatc aaactcgtcc cagccgaggt caaatccata
     6481 ccgctctgct gccgcatcga gcacctttcg cccttctgcg atgatctcag ggccgatccc
     6541 gtctccgcct attgctgcga cccggtacac cgatccacct cctcgagatt ccttgcaaag
     6601 gggataagac ccccggcatc agcgatccgg agcaggaacc cagggacagg ctcaatcgga
     6661 tacgttctct ctccacaggt tatcaggtgc ttatcaatat ccaccctgca ctcatcacca
     6721 tcagggattg c
//
LOCUS       DAOOCO010000102         6656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10780_length_6656_cov_4.540979, whole genome shotgun sequence.
ACCESSION   DAOOCO010000102 DAOOCO010000000
VERSION     DAOOCO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10780_length_6656_cov_4.540979"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK154_06650"
     CDS             complement(<1..448)
                     /locus_tag="PK154_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW10777.1"
                     /translation="MRSHCTEKNLAIVILGCIICCAGALAVPTTDLLVSEYGADGTPS
                     MNKSVTIQWMEANLPVQGDGTTHYYHQGPVFVESKEGQWDRNETTNFKDMGAVKGTAV
                     RDLCDLVGGMNAGDDVMVKAVDGYHVEIPYENIYYPDPRQGNITVCW"
     gene            complement(445..1302)
                     /locus_tag="PK154_06655"
     CDS             complement(445..1302)
                     /locus_tag="PK154_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW10778.1"
                     /translation="MAPVHAHPYEPFTGADVPALLCLFLVAGVFFLAFPASAATTSLH
                     IEKIAWDGTTILSSTDVDAAWMETHLPVYGDGVTHYYHQGPVFVDDPDPVTEEMIRWN
                     AAEDTNVQEKDMGAVKGTAVKDLVSLAGGMEPGDTVTIRAVDGFSKTFGYRNVYLPPD
                     RQGPMVIAWYREDEGYVPEYSTGMRLVFLADAATNPWGIYAFGNWDWHESADPEYYYY
                     YAQGNERYPTTTGLSVQMVSSLSIRQASNMTVPDTVPFTPSVSLGILTIASGMLAGGW
                     IVLRTGGTR"
     gene            complement(1598..2518)
                     /locus_tag="PK154_06660"
     CDS             complement(1598..2518)
                     /locus_tag="PK154_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPW10779.1"
                     /translation="MILAFVLFIIGLILLLKGAEVAVSGAEGLAFRMGVSATTIGLTV
                     VAFGTSLPEFFVTTEAFYTGTYEIGLANITGSNIANIGLILALCVLLNPSITSSRHLR
                     PRFLKDSLLMLAATLLYAVLSLRGLIDWITGLAFLVLFFVILFFIRKNCHDDMTNPPA
                     ETRYPLVKTVLGLGAVILGSHLLLTSAVDLAAILGIPEYVIGLSLVAIGTSLPELATS
                     LIAAVKNSPAISVGNIVGSNIFNILFILGINSLFTTVPIPGYTDTLVMTGFAAGIFGL
                     FFGSRLQNRAWALVLLVSYVIFIAMVFGAW"
     gene            2677..2964
                     /locus_tag="PK154_06665"
     CDS             2677..2964
                     /locus_tag="PK154_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10780.1"
                     /translation="MVPGTAIVQDRSRFEKTCTSPFFSAYPEACSSGCGRVPYKGTCC
                     HNLIAVYLNPGIVIPGQFPEKIVGAKGMNRSKEKSGSGKEGETWGFRGFPG"
     gene            complement(2945..4135)
                     /locus_tag="PK154_06670"
     CDS             complement(2945..4135)
                     /locus_tag="PK154_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10781.1"
                     /translation="MNRITQCMHGRGTVSEVMKHRHGQPGEVPSRYLAFSGMPRPVIF
                     WNLTDRCNLSCVHCYSRSGPGHDITGELTTGEALDLIDDLARSGIPLILFSGGEPLLR
                     HDLWELARHARDRGIRTALSTNGTLIDTEIAGKIRESGIGYVGISLDGATAATHDRFR
                     GTPGAFDLSIAAFSHCLDSGVRCGVRVTLTRENAGELGALIDLARRIGASRFCLYWLV
                     PSGRGREGYARLQLGSQEVQDALDLLYRTAKETAPGAMEFLTVDAPQDCIHLLGSMKQ
                     DSSQDLQDAEQLLESLNGGCSAGIRVANIDPMGNIYPCQFARAPEFLIGNIRARPFSS
                     IWSDDTNQVLSRFRNKKASIGGRCSTCPYFELCGGGCRVRAYAVSGNFFDEDPFCFVN
                     PENP"
     gene            complement(4132..4620)
                     /locus_tag="PK154_06675"
     CDS             complement(4132..4620)
                     /locus_tag="PK154_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW10782.1"
                     /translation="MEPRDLQVLAALEVGLPLVPDPYGEIGRKLGMSGDEVLERVQTL
                     RDAGIIRRFRARINQRLLGITANALVAWHCNGQAAEQAGAILSAFPGVTHCYERQPVP
                     GRWDYTLYTVHHGFSREEVQEEVRMIADRAGLHEYVVLFSTDEFKRVPNVRIRENGGG
                     IG"
     gene            complement(4607..5116)
                     /locus_tag="PK154_06680"
     CDS             complement(4607..5116)
                     /locus_tag="PK154_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPW10783.1"
                     /translation="MNAPPQTDALDIRILDALQQEIPLVARPWEDLAGRLGVPESVLL
                     ERLERLRHAGILRGISPIIESRPLGLTAATLVALPVPGKRIREVAALISEYPEVSHNF
                     RRDHHYSLWFTLSAKDTRSLHAVLEEILTRTGFSDTDILNLPTIRKIKIDVRFPLTGK
                     QNGGIDGTA"
     gene            complement(5113..6213)
                     /locus_tag="PK154_06685"
     CDS             complement(5113..6213)
                     /locus_tag="PK154_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10784.1"
                     /translation="MQGKRAGAVKDLPLGPPRLISWNITLRCPLKCTHCYVRAGEHEA
                     AGVLSTDEALGVIDQIRHVGTPVVVLSGGEPLMRTDLCTIARYGTDRGLRMVMGTSGY
                     FLDRAMAARMRDAGIRAVAVSIDSADPAVHDSFRGVSGAWEKAVQGIQNCRDEGIGVQ
                     INMTVMRPSAGDVQSVVELGTMLGVADYQVFFPIPTGRAQGADPLDPLSYEEVIRQVL
                     LKYRDSRVNLRPTCAPQFLRIAAELGISHPAWGRGCIAGIRYCRIYADGEVTPCPYLP
                     VSAGNVRKTPFREIWEHSPIFSALRDPDLLTGKCGRCEYKVICGGCRARAYRGMEVVS
                     PRWCDGLEKPGVTAGDLCSEDPWCPYEPGGES"
     gene            complement(6271..>6656)
                     /locus_tag="PK154_06690"
     CDS             complement(6271..>6656)
                     /locus_tag="PK154_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449392.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HPW10785.1"
                     /translation="LLTLTAPEMTVLIGGMRVLDTNVGQTRHGVFTERPEALTNDFFV
                     NLLDMGTEWKAVSNEDDLFEGRDRKTGEVRWTGTRVDLIFGSNAQLRALAEVYASADA
                     KGKFIEDFVAAWTRVMNLDRFDLHR"
BASE COUNT         1432 a         1950 c         1884 g         1390 t
ORIGIN      
        1 accagcacac ggtgatgttc ccctgccggg gatcagggta gtaaatgttt tcataaggga
       61 tctcgacgtg atatccgtca actgccttta ccatcacgtc atcaccggca ttcatccctc
      121 cgacaagatc gcagaggtca cggacggccg tacctttcac agcacccata tccttgaagt
      181 ttgtcgtctc gttcctgtcc cactgcccct cttttgattc gacaaagacc gggccctggt
      241 gatagtagtg ggtcgttcca tcaccctgca ccgggaggtt tgcctccatc cactggatgg
      301 ttacgctctt gttcatagag ggggtcccgt ctgctccata ctccgagacg agcagatcgg
      361 ttgtcggcac ggcaagggcc ccggcacaac agatgatgca gcccaggatc actatcgcca
      421 ggttcttttc tgtacaatgt gatctcatcg tgtacctcct gttcgcagga caatccatcc
      481 tcctgcgagc attcctgaag caatcgtcag gataccaagg gaaacagagg gtgtgaaagg
      541 tacggtatca ggaacggtca tattcgacgc ctggcggatg ctcagggaag agaccatctg
      601 cactgataac cctgttgttg tagggtatcg ttcgttgcct tgtgcgtagt aataataata
      661 ctctggatcg gccgattcat gccagtccca gtttccgaat gcatagatac cccagggatt
      721 cgttgctgca tcggcaagga agacaagcct cattcctgtt gagtattcag ggacatagcc
      781 ctcgtcttcc cgataccagg caatcaccat cggcccctgg cgatcagggg gcaggtagac
      841 attccggtac ccaaacgtct tggagaatcc atcgacagcc ctgatggtga cggtgtcacc
      901 cggctccatt ccaccggcaa gactgaccag atcctttacc gcggtccctt tgacggcacc
      961 catgtccttc tcctggacat tggtgtcctc tgcagcattc caacggatca tctcttcagt
     1021 taccgggtcg ggatcatcga cgaagaccgg cccctgatgg taataatggg tcaccccatc
     1081 accatacacg ggaagatgtg tctccatcca ggcagcatca acatcagtcg atgagaggat
     1141 ggtcgttcca tcccatgcaa tcttctctat atgaagcgat gtcgtggccg ctgatgccgg
     1201 aaaagccaaa aaaaatactc ctgcaacgag gaaaagacag aggagagctg gcacatctgc
     1261 accggtgaat ggttcgtagg gatgagcgtg gacgggtgcc atggagatgt cagagttgca
     1321 tgccaccccg gatataattt tttgtttctc cttaaaacat tcaatatatt aacttaatgt
     1381 cattactctg atcccctgct gccatcagga gcaccgtgtt agccgccctt ggctaatcga
     1441 gaaagagtac ccctcggaaa tttcacaacc agttccacag agcagagtac ctcctttccc
     1501 ttgtcagaac tttctgaacc ctgtatcctc ctccaggata tcctgactca ttgtggcaga
     1561 ccgggcagtc atgacggaca atcagggacc tcactgatta ccatgcacca aaaaccatcg
     1621 caatgaagat cacataggag acgagcagaa caagagccca ggcccggttc tgcagtctgc
     1681 tcccaaaaaa aagcccgaag atcccggctg caaatcccgt catgacaagg gtgtcggtat
     1741 atcctggtat cggaacggtg gtaaagagcg agtttatccc gaggatgaag aggatattga
     1801 agatattgct gccgacaatg ttccccaccg agatggccgg actgttcttg acggcggcaa
     1861 tgagcgaagt ggcaagctcg gggagagagg ttccgatagc gaccagggac aggcctatga
     1921 catattcagg gatacccagg atcgctgcaa ggtcgaccgc ggaggtgagg aggagatggg
     1981 atccaaggat gactgctcca agcccaagga ccgttttgac caggggatac ctcgtttcgg
     2041 cagggggatt cgtcatatca tcgtggcagt tctttctgat aaaaaagagg ataacgaaga
     2101 acagaaccag gaatgcaagc cccgtgatcc agtcgatcag ccccctcagg gaaagcaccg
     2161 catacaggag cgttgcagca agcatcagga gcgaatcttt caggaaacgg gggcgcaggt
     2221 gccgggagga tgtgatggag ggattgagaa ggacacagag ggccaggatg agaccgatgt
     2281 ttgcgatatt gctcccggtg atgttggcaa gcccgatctc gtaggtgccg gtgtaaaatg
     2341 cctccgttgt gacaaagaat tcggggagtg aagtgccgaa tgcgaccact gtgagcccaa
     2401 tcgtggtcgc tgacactccc atccggaatg caagaccttc tgctccactg accgcaacct
     2461 ccgcaccttt cagcagcagt atcaacccga tgatgaagag gacgaatgcc aggatcattc
     2521 cagtgattgg atatcgcgct gatggggaag atggtatcgg cctgatataa caggaaaaga
     2581 ccgatggtga cctgtccctc ctgaataggg atcaggtcat gatccgggac aagtctgttt
     2641 tccgggggat gatcccggtt ccgatccctc gtcccattgg tcccaggaac cgcgatagtc
     2701 caggaccggt cccgattcga aaagacctgc acatccccct tttttagcgc ctatcccgaa
     2761 gcatgttcgt ccgggtgcgg ccgggtccct tataagggca cgtgctgcca caacctcatt
     2821 gcggtatacc tcaacccggg gattgtcatc ccgggccagt tccctgaaaa aatcgtgggg
     2881 gcaaagggta tgaaccgctc aaaagaaaag tccggatcag ggaaggaggg ggaaacctgg
     2941 ggattcaggg gttttccggg ttgacaaaac agaagggatc ttcatcaaaa aaattccccg
     3001 aaacggcata agcccgcacc cggcatcctc ccccgcacaa ttcaaaatag gggcacgtgc
     3061 tgcatctccc gccgatggat gcttttttgt tccggaacct ggacaggacc tggttcgtat
     3121 cgtccgacca aatcgatgag aatggccgtg cccggatatt tccgatgagg aactccggag
     3181 ccctcgcaaa ctggcagggg tagatgttcc ccataggatc gatatttgcc acccggatgc
     3241 cggcactgca gcccccgttc agggattcga ggagctgttc cgcatcctgc aggtcctggg
     3301 atgagtcctg tttcatcgaa ccgagaagat gaatgcagtc ctgcggggca tcaacggtca
     3361 gaaattccat agcccccggg gccgtctctt tcgcggtccg gtagagcaga tcgagggcat
     3421 cctgtacttc ctggctgccg agctggaggc gtgcataacc ctccctgccc cgcccgctcg
     3481 ggacgagcca gtagaggcag aaccgggaag ccccaatacg ccgtgcaagg tcgataaggg
     3541 cgcccagttc accagcattc tcccgcgtca gcgttacccg caccccgcac ctgacgcccg
     3601 agtcgaggca gtgggaaaac gctgcgatcg ataggtcaaa tgcaccgggg gtccccctga
     3661 accggtcatg ggtggcggcc gttgcaccat ccagtgagat accgacgtat ccaatcccgc
     3721 tttcacgtat ttttcccgct atctccgtgt caatgagagt accattggtg ctgagcgcgg
     3781 tcctgatccc tcgatccctg gcatgacgcg caagctccca gaggtcatgg cgcaggagtg
     3841 gctctccccc ggaaaagagg atgaggggga tccccgatcg cgcaaggtca tcgatcaggt
     3901 ccagtgcctc ccccgtggtc aattcaccgg tgatatcgtg accaggcccc gagcggctgt
     3961 agcagtgcac gcaggagagg ttgcagcggt cggtgaggtt ccagaatatg accggtcgtg
     4021 gcatgccgga gaatgcaagg taccggctcg ggacctcacc gggctgtcca tggcggtgtt
     4081 tcatcacttc actcacggtg cccctgccat gcatgcactg cgtgattcgg ttcacccgat
     4141 acctcccccg ttctccctga tccggacgtt cggtacacgc ttgaattcat cggtgctgaa
     4201 aaggacaaca tactcgtgaa ggcctgccct gtctgctatc atccggacct cttcctgcac
     4261 ttcttcccgt gaaaagccgt ggtggaccgt gtagagggtg tagtcccacc ttcccggaac
     4321 cggctgtcgt tcataacagt gggtgacccc gggaaaggct gacaggatcg ctccggcctg
     4381 ctccgcggcc tgtccattgc aatgccatgc gacaagggca tttgcggtga taccgaggag
     4441 acgctggttg atccgtgccc ggaacctgcg gatgatgccg gcatcccgca gtgtctgcac
     4501 tctttcaagg acttcatcac cggacatacc gagctttctg ccgatctccc catagggatc
     4561 agggacaagg ggaagaccta cttcaagggc ggcaaggacc tgcaggtcac gcggttccat
     4621 cgatccctcc gttctgcttc cctgtgagtg ggaaccggac atcgatcttg atcttccgga
     4681 tggtcggcag gttgagaata tcggtatcag aaaaccccgt ccgtgtgagt atttcctcga
     4741 gaactgcgtg gagtgaccta gtatcctttg ccgagagggt aaaccagagt gagtaatggt
     4801 ggtcgcgccg gaagttgtgc gacacttcag ggtattcgct gatcagggcg gccacctcgc
     4861 ggattcgttt tccgggcacc ggcagggcga caagcgtggc agcagtgagg cccagaggcc
     4921 gggattcgat aattggagag attccccgca ggatcccggc atggcggagc cgctccagcc
     4981 gttccagaag cacggattcc gggacgccga gcctgcctgc caggtcttcc catggccggg
     5041 caacgagcgg gatctcctgc tggagtgcat cgaggatgcg gatgtcgagg gcgtcagtct
     5101 ggggtggagc gttcatgact cccctcccgg ctcgtagggg caccaggggt cctcagagca
     5161 taggtctcct gctgtcaccc ccggcttctc aaggccgtca caccagcggg gggaaaccac
     5221 ttccattccc cggtatgccc gggcacggca gccgccgcat atgaccttgt attcacaccg
     5281 gccacatttt ccggtcagca ggtcgggatc acggagggca gagaagatcg gtgaatgttc
     5341 ccatatctcg cgaaacggtg tctttcggac attaccggca ctgactggca ggtagggaca
     5401 gggggtgacc tccccatccg cgtagatacg acaatagcgg atcccggcga tacagccccg
     5461 cccccatgcc gggtggctga tcccgagctc cgcggcaatc cgcaggaact gcggggcaca
     5521 ggtcggtcgg aggttcacac gggagtcccg gtacttcagg agcacctggc gtatcacctc
     5581 ttcatatgac aggggatcca acgggtctgc accctgcgcc cgtcccgtgg ggatcgggaa
     5641 gaagacctgg tagtccgcca cccccagcat ggttccgagc tccacgactg attgcacgtc
     5701 ccctgccgag ggccgcatca cggtcatgtt gatctggaca ccgatgccct cgtcgcggca
     5761 gttctgtatt ccctgcactg ccttctccca ggcgccgctg acgccacgga acgagtcatg
     5821 gacggcagga tctgccgagt ctatgctgac ggcaacagcc cgtatccccg catccctcat
     5881 ccgtgctgcc atcgcacggt cgaggaagta cccgctggtg cccatcacca tccggagccc
     5941 gcgatcagta ccatagcggg caatggtgca gagatcggtc cgcatgagtg gctctccccc
     6001 ggagaggacg acaacaggcg tcccgacgtg ccgtatctgg tcgatcaccc ccagtgcctc
     6061 gtccgtggag agcactcctg ccgcttcgtg ctcccctgct ctcacgtagc agtgggtgca
     6121 ctttagggga catctgagcg tgatattcca cgagatgaga cgcggcggtc caagcggcag
     6181 atccttcact gcccccgccc gtttcccctg cactgccggg gttccttatc tgcattcgtg
     6241 aggtgttccc ccattgaggg tactgtgcta tcaccggtgg agatcgaagc gatcaaggtt
     6301 catcaccctg gtccaggctg ccacgaagtc ctcgatgaac ttcccttttg cgtctgcgct
     6361 tgcatagacc tctgcgagag cccggagctg ggcattcgaa ccgaagatca ggtcgactcg
     6421 cgtgccggtc cacctgacct ctcccgtctt tctgtcgcgc ccttcaaaga gatcatcttc
     6481 attcgagact gctttccact ccgtgcccat gtcgagcagg ttcacgaaga agtcgttcgt
     6541 gagcgcctcc ggccgttcgg tgaagacgcc atgccgcgtc tgtcctacat tggtgtccag
     6601 cacccgcata cctccgatga gcacggtcat ctcgggtgcg gtgagggtca gcagtt
//
LOCUS       DAOOCO010000103         6640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10824_length_6640_cov_4.729841, whole genome shotgun sequence.
ACCESSION   DAOOCO010000103 DAOOCO010000000
VERSION     DAOOCO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10824_length_6640_cov_4.729841"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PK154_06695"
     CDS             <1..837
                     /locus_tag="PK154_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10786.1"
                     /translation="PSGIDRPYNIAHPELASRPFIGGISGDPELVALQKPDVVFVTFV
                     TGKDADALQEKSRVPVISLISGDLGKNRQVFYSSLRTMATVLGKEQRAEDVISYINAT
                     LADLQERTSGIPAGKKASVYVGGIAYNGAHGLLSTDPAYAPLAMVNGNNVAAEAGTGG
                     QIMIDKEKLLSWNPDVIFIDEASYSLVVEDLKDPVYQSLDAVREGRVYGVMPYNWYAN
                     NYDTVLADAYFIGKILYPDQFVEVDPEQKADEIYTMLDGKPVYAEMKQIFGGFVPFSQ
                     IS"
     gene            848..1924
                     /locus_tag="PK154_06700"
     CDS             848..1924
                     /locus_tag="PK154_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW10787.1"
                     /translation="MVPSSSNPFSLEETPASGTEAPLHFLEKALGGLRLFQAFVTAYE
                     GGIFEACREPASLAAICGKTGAHPGIAGPLCEGLVRTGVLSHDRSGNYQVTAATRDFF
                     LPDSRFSQKAAIAQMKEFASLFSRLPDILPKGPVVYPRGEQFRERVIPAMAEQSRCGL
                     VQRVAGIVSSLPEFPSARWLLDLGGGHGLYAIALTRKNPDLSAVVFDLPEVTPVTREI
                     IRRYGAQERITTHDGDFFRDPLGTGYDILFSSSNPSGKMPALIPKISDALNPGGLYLN
                     KQVIDSPEEDPFADLEWNLWALEGMKKKEKRFRFEGSVRMEEYNRMLGESGFVVRNTV
                     PVDGRSVMTIAQKAGVRGAGHDAS"
     gene            1914..2987
                     /locus_tag="PK154_06705"
     CDS             1914..2987
                     /locus_tag="PK154_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW10788.1"
                     /translation="MHLDVNPLHEPEGYLRYVRRKGLFLIGAGFGLVLMFLVALAVGS
                     SFIPLPEVVAAFLSPSGEHISTIVLHIRLPRVLSAVIAGAGLAAAGVVMQSVLRNPLS
                     SPYTLGVSHAAAFGAAFAITALGAGSISHLISDSMVVRNPYLITICAFLGAMLATAGL
                     LLVAKYRSSRPEVMILVGVALAALFTAGTTFLQYFSSADQIAAIVFWTFGDVGRATWR
                     DLLLMMVIVLPSLGYFFFTRWDYNAIDAGDETAHTLGVNVERVRTLAMLLSSLITAVV
                     VSFLGIIGFIGLVSPHMMRRVVGCDQRYLLPASCIAGAILLLGADMVARLIIAPAVLP
                     VGVLTAFLGAPLFLYLLIGRSVV"
     gene            2984..3778
                     /locus_tag="PK154_06710"
     CDS             2984..3778
                     /locus_tag="PK154_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10789.1"
                     /translation="MILKVAGLSFRYNGLKILEEVEFRVNRHEILAILGPNGVGKTTL
                     LRCINTLLRPQAGSILLDDQDLLSLGRDEVARLLAYVPQRGETGPTLAFDAILLGRRP
                     HIGWDVRREDIQKVQAIINQLHIGHLALRPINSMSGGELQKVLIARALVQEPGLLLLD
                     EPTSSLDLKNQIEILAFLRMIVRAHSLTAILTMHDINQAIRFADRFLFLKEGMVYAAG
                     TREIITPELIREVYGLPVTIAEVNRMPVVVPGLDHLQATETSLSAE"
     gene            3885..4331
                     /locus_tag="PK154_06715"
     CDS             3885..4331
                     /locus_tag="PK154_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPW10790.1"
                     /translation="MGDAIKLGIIICDRYRTCAGGKCLRALRNREGAFARYQGKEVEL
                     VGFATCNGCPGGNIEYVPQEMKKNGAEVVHLATGMIVGYPPCPRISYFKKFLREKYGL
                     EVIVGTHPIPQNYYMTHTKLGTWEGAAWKELVSPTLTDEKTRLTYD"
     gene            complement(4426..4971)
                     /locus_tag="PK154_06720"
     CDS             complement(4426..4971)
                     /locus_tag="PK154_06720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPW10791.1"
                     /translation="MSCTPIRTGRLELVQASLEMLISNQGRRTLLGRLLNAAIPLSWP
                     PPFLDKETLAEFARIQSEGSDQNFCSWYRIRCGDERNDRTLIGSGGIASVQGEPDTVM
                     IGYSVLGAYWNQGYATEVVRHLVPVILDTPGIRRITATTYPEMTVSIRVLEKNGFIRA
                     GIGSGGKGREEGTVRYFLTRR"
     gene            complement(5086..6471)
                     /locus_tag="PK154_06725"
     CDS             complement(5086..6471)
                     /locus_tag="PK154_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HPW10792.1"
                     /translation="MAERGPDACLEGILAIPGERRWAIDSICIVKGVTAYMVHSFDGT
                     ALLFSLPLLIRRSLPEESPLDIRGAAGAVAMIIEQIKQKAPSERRLGPLTGQGTRFPH
                     QVASITEPYTLVASPAALASDLWHADRRNYADRDGIHLLLHGAVPAPVEYAPGSITTI
                     TETVTNLCESVTAIAFSVPARELETAWIGTLDQQLLRESLPSLGLVSFVGDESRLARQ
                     YTRYRPFFRTAGPREEVNIPFTCPRDLEPVEIELPVSNRSVTGLGIRQREVFAVAGSN
                     AQGKTTFLDGILAGMDDHAAGDGREHVVTVPGLCSAEAMTSLMTGADISMFFSALPPG
                     INGTPRAASGMGSGSMNMAYQVQRAVERGSPLLIIDEDRAAANLLVRSCLQTGEITPL
                     SEILASDRGKMGETALVFAACAMDTLVAQADRIMVLDRHVAYAVDRESFREKVAESLE
                     GMAGDLRKGSQ"
     gene            complement(6440..>6640)
                     /locus_tag="PK154_06730"
     CDS             complement(6440..>6640)
                     /locus_tag="PK154_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10793.1"
                     /translation="QHGRTSPCFYIKIMRNRFAMNWEGVPGGLVDPTMTMAEQEGRKP
                     SIYYRGRMIPSYHGRAGAGCLS"
BASE COUNT         1267 a         1965 c         1997 g         1411 t
ORIGIN      
        1 ccctcgggga ttgatcgacc gtataatatt gcccatcctg aacttgccag ccgccctttt
       61 atcgggggga taagcggcga tcctgaactt gttgcgctcc agaagcccga tgtggtgttc
      121 gtgacgttcg tgaccgggaa ggatgccgat gccctgcagg agaagagcag ggtaccggtc
      181 atctcgctca tctcgggcga cctcgggaaa aaccggcagg tgttctactc atccctgcga
      241 accatggcca cggtccttgg caaagagcag cgtgccgagg atgtcatcag ctatatcaat
      301 gccaccctgg ccgatctcca ggagcggacc agcgggatcc ctgctggtaa gaaggcctcg
      361 gtctatgttg gcgggatcgc ctataacggt gcccatgggc tcctctccac agatcccgcg
      421 tatgcccccc ttgccatggt aaacgggaac aatgttgcag ccgaggccgg gactggtggc
      481 cagatcatga tcgacaagga gaaactgctc tcatggaacc ccgatgtcat attcatcgat
      541 gaggccagtt attccctcgt ggtcgaggac ctgaaggacc ccgtgtacca gtccctggat
      601 gccgtcaggg aggggagggt ctatggggtc atgccctaca actggtacgc caacaactac
      661 gacacggtgc ttgccgacgc ctacttcatt gggaagatcc tgtatcctga ccaatttgtg
      721 gaggttgacc cggaacagaa ggctgacgag atttacacca tgcttgacgg gaaaccggtg
      781 tatgcggaga tgaagcagat cttcgggggc tttgtgccct tcagccagat ctcatgaggg
      841 atggttgatg gtcccttcct cttccaatcc cttctcgctc gaagagactc ctgcatcagg
      901 aaccgaggca ccccttcact tcctcgagaa ggcgctcggt ggactgcgat tgttccaggc
      961 ctttgtcacg gcatatgagg gagggatctt tgaggcctgc agggaacccg catcgctggc
     1021 ggctatatgc gggaaaacgg gggctcatcc cggaatcgcc ggacctctct gcgagggact
     1081 ggtgcgtacc ggtgttctct cccatgaccg atcagggaac taccaggtca cggcagccac
     1141 ccgcgatttc ttcctccccg attcccggtt cagccagaag gctgcgatcg cccagatgaa
     1201 ggaatttgcc tcgctcttct cccgcctccc ggatatcctg ccaaaaggcc ccgttgtgta
     1261 tccccggggg gagcagttca gggagcgggt catcccggcc atggctgaac agagccggtg
     1321 cggactggtg cagcgggtcg ccggaattgt ctcttccctc cccgagttcc cttctgcacg
     1381 gtggttgctc gacctcggag gcggtcacgg actgtatgct atcgccctca cccggaaaaa
     1441 tcctgatctc tcggccgtgg tctttgatct gcccgaggtg accccggtaa cccgggagat
     1501 aatccggcgg tatggagcac aggaacggat cacgacccac gatggtgact ttttccgtga
     1561 tccccttggt acgggatatg atatcctctt ctcttcgtca aacccctccg ggaagatgcc
     1621 tgccctgatc ccgaagatct ccgatgccct caatccaggc gggctctatc tcaacaagca
     1681 ggtgatcgac agccctgaag aggatccctt tgccgacctt gaatggaacc tctgggcgct
     1741 ggaagggatg aaaaagaagg aaaagcggtt tcggttcgag ggcagcgtcc gcatggagga
     1801 gtacaaccgc atgctcggcg aatcgggatt cgtggtccgg aacaccgttc cggttgacgg
     1861 ccggtcggtc atgaccatcg cacagaaagc aggtgttcgg ggagcagggc acgatgcatc
     1921 ttgatgtgaa cccgctgcac gagcctgaag gataccttcg gtatgtccgc cgcaagggat
     1981 tgttcctcat aggggcaggg ttcggcctcg tgctgatgtt cctggtcgca ctggcggtcg
     2041 ggagctcgtt tattcccctg ccggaggtgg tcgcagcctt cctctcacct tcgggggagc
     2101 atatctctac cattgtcctg catatccgcc ttccccgggt gctctccgcg gtgatagccg
     2161 gggccgggct tgctgcagcc ggggtggtca tgcagtccgt gctcaggaac ccgctgagct
     2221 ccccctacac gctgggagtc tcccatgccg cagcattcgg agcggcgttt gcgatcaccg
     2281 ctctgggggc cggttcgatc tcccacctca tctcggattc catggtcgtc cgaaacccct
     2341 acctgatcac catctgtgcc ttcctcgggg cgatgcttgc caccgcgggt cttctcctcg
     2401 ttgcgaaata ccggagttcc cggccggagg tgatgatcct ggtcggggtg gcgctcgccg
     2461 ccctcttcac agcggggacc acgttcctcc agtatttctc atcggctgac cagatcgcgg
     2521 cgatcgtctt ctggacattc ggggatgtcg gccgggcaac ctggcgtgat ctcctgctca
     2581 tgatggtgat cgtcctgcca tccctcggat atttcttctt cacccggtgg gattacaatg
     2641 ccatcgatgc gggggacgag accgcccata ctctcggggt caatgtggag cgggtccgga
     2701 cgcttgcgat gctgctctcc tcgttgatca ccgctgtcgt ggtatcgttc ctcggcatca
     2761 tcgggttcat cgggctcgtg agcccccata tgatgcgccg ggtggtgggg tgtgaccagc
     2821 ggtatctcct ccctgcatcc tgtatcgcgg gggcgatcct cctgcttggc gccgacatgg
     2881 tcgcccggct gatcatcgcc ccggcggtcc tcccggtcgg tgtcctgacg gcgtttctcg
     2941 gcgccccgct ctttctctac ctgcttatcg ggaggagtgt ggtatgatcc tgaaggtagc
     3001 cgggctcagc ttccggtaca atggcctgaa gatactcgag gaggtggagt tccgggtcaa
     3061 ccgccacgag atcctcgcca tcctcggtcc gaacggcgtc ggaaagacca ccctgctccg
     3121 gtgcatcaac acactgcttc gtccccaggc agggagtatc cttcttgatg accaggatct
     3181 cctctcgctg gggagggatg aggtggcacg gttgcttgcc tacgttccgc agcggggcga
     3241 gacaggccca accctggcat tcgatgccat cctgctcggg cgacgcccgc acatcggctg
     3301 ggatgtgcgg agagaggata tccagaaggt ccaggccatc atcaaccagc tgcacatcgg
     3361 acacctggca ttgcggccga tcaattccat gagcgggggc gagctccaga aagtcctgat
     3421 cgcacgggcg ctcgtgcagg agccgggcct cctcctcctc gacgaaccga caagcagcct
     3481 cgacctgaag aaccagatcg agatccttgc cttcctccgc atgatcgtcc gggcccattc
     3541 gctcacagca atcctgacca tgcatgatat caaccaggct atccggttcg ccgaccggtt
     3601 cctctttctc aaggagggga tggtctacgc tgccgggaca cgggagatca tcaccccgga
     3661 gctgatccgg gaggtgtatg gtcttcccgt caccatcgcc gaggtcaacc ggatgccagt
     3721 ggtggtgccg ggtctcgatc atctccaagc aacggaaacc tcgctgtcag ctgagtgaga
     3781 gcggggaatt cttcctgggt tcctcgtttg tgcagtgcca tgggtatttc tttgtgaaga
     3841 gaatacccct aaggtaccag aaagcattag ccggaggaag tgtcgtggga gacgcgataa
     3901 aactaggaat catcatatgc gatcggtacc ggacctgtgc cgggggcaaa tgcctgcggg
     3961 cgctccgcaa ccgcgaggga gcgtttgccc ggtaccaggg aaaagaggtc gagctggtcg
     4021 ggtttgccac ctgcaacggg tgcccgggag gaaacatcga gtatgtaccc caggagatga
     4081 agaagaacgg ggcagaggtt gtccatcttg ccaccgggat gattgtcggc tacccgccct
     4141 gtccgcggat ctcctatttt aagaagtttc tccgggagaa gtatggactt gaggtgatcg
     4201 ttgggacgca tcccattccg cagaattact atatgaccca tacgaagctt ggcacctggg
     4261 agggagcggc gtggaaggaa ctggtttcac cgactcttac cgacgagaag acccggctga
     4321 cttatgattg aatggcatgg gtgggataaa aaaaaccatt ctttcgtcgc gaaacctgtc
     4381 ctgtccgttt attatgtgtt tcaatagagc gggattcctg attctttacc gcctggtgag
     4441 aaaataccgt accgtacctt cctccctgcc ttttcctcca ctccctatcc cggcgcggat
     4501 aaaaccgttc ttttccagga cacggattga tactgtcatc tccgggtagg tggtggcggt
     4561 gatccgccgg atgcccggag tatccaggat gacagggacg aggtggcgga ctacttcggt
     4621 ggcatagccc tggttccagt acgctccgag caccgagtac ccgatcatca cggtgtccgg
     4681 ttctccctgg accgatgcaa ttcctccgct gccgattagg gtgcgatcgt tccgctcatc
     4741 tccacaacgg atccggtacc aggagcagaa gttctgatcc gatccctctg actggatcct
     4801 ggcgaattct gccagagtct ctttatcgag gaacggtggc ggccacgaca gagggatggc
     4861 agcattgagg aggcgcccga gcagggtgcg gcgcccctga ttactgatca gcatctcgag
     4921 agatgcctgg acaagttcga gacgcccggt ccggatgggt gtgcaggaca acaggaaact
     4981 tcaccagtac tgatctggct gtgttggaag ggcagatatc ttacggtcgc ccatgccgca
     5041 tgcgtatctg gtattatcac aggcaggaat ggtggagtat gccgttcatt gactcccttt
     5101 ccttagatcg cctgccatcc cttccagcga ttcagccacc ttctcccgga acgattcccg
     5161 gtcaacagca tacgcaacgt gccggtcaag gaccatgatc cggtctgcct gggccaccag
     5221 ggtatccatg gcacacgcgg caaagacgag cgctgtctcc cccatcttcc cgcggtcgct
     5281 ggccaggatc tctgacaggg gggttatctc cccggtctgc aggcagcttc ggaccagcag
     5341 gttggcagct gcccggtcct catcgatgat caggagcggg gaaccccgct cgaccgcccg
     5401 ctggacctga tacgccatgt tcatcgatcc gctgcccatg ccggaggcgg cccggggagt
     5461 tccattgatt cccggaggga gtgccgaaaa gaacatactg atatcagccc cggtcatgag
     5521 ggaggtcatt gcttcggcac tgcaaagacc aggcactgtc acgacatgct ccctcccgtc
     5581 cccggcagca tgatcatcca tgcccgctag gatcccgtca aggaacgtgg tcttcccctg
     5641 ggcatttgat ccggcgaccg cgaagacctc cctttgccgg atcccaagac cggtaacgga
     5701 ccggttgctg accgggagct cgatctcgac tggctcgaga tccctggggc aggtaaaagg
     5761 gatgttcacc tcctctcttg gcccggcggt ccgaaagaag gggcggtacc tggtgtactg
     5821 gcgggctagt cgtgactcat ccccgacaaa ggagacaagg ccgagcgaag gaagggattc
     5881 cctgagcagc tgctggtcga gcgttccgat ccaggccgtt tccagttccc gggccgggac
     5941 cgagaaggcg atggcggtga ccgattcgca caggttcgtg acggtttcag tgattgtggt
     6001 gattgaaccg ggagcgtact ctacgggagc ggggactgca ccatggagaa gaaggtggat
     6061 cccatcgcgg tccgcataat tccggcggtc ggcatgccag aggtcggatg ccagggcagc
     6121 gggagatgcc acgagggtat acggttcggt gattgaggcg acctggtggg ggaaccgggt
     6181 gccctggccg gtgagcggcc cgagcctacg ctcggagggt gccttctgct tgatctgctc
     6241 aatgatcatg gctaccgcac ctgctgcacc gcggatgtca agcggactct cctccgggag
     6301 ggaacggcgg ataagcagcg ggagggagaa gaggagtgcc gtaccgtcaa atgaatggac
     6361 catgtatgca gtgactccct tcacaatgca gatgctgtct atcgcccacc gtctctcacc
     6421 cggaatggcg agaatcccct caagacaggc atccggcccc cgctctgcca tggtacgagg
     6481 gtatcatcct ccctcggtaa taaatcgagg gtttgcgtcc ctcttgttct gccatggtca
     6541 tggtgggatc gaccagacca ccggggaccc cctcccagtt catcgcgaac ctgtttcgca
     6601 tgatttttat gtaaaaacag ggcgatgttc tcccatgctg
//
LOCUS       DAOOCO010000104         6636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10834_length_6636_cov_4.767209, whole genome shotgun sequence.
ACCESSION   DAOOCO010000104 DAOOCO010000000
VERSION     DAOOCO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_6636_cov_4.767209"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK154_06735"
     CDS             complement(<1..787)
                     /locus_tag="PK154_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPW10794.1"
                     /translation="MADSAQNGSRERVLYAVLGCGSTGYHVLQELQSSTGNVLVIDKN
                     EDRVRELRDQRIDAVRRDISALDFLSGIPAIEIAFVLTGDNEANLAAVKALRVAYPHS
                     RIMARATDPLVARNLEEAGADFVLNQNQLMAKAAVYQINKVYANRVSQRLYSLLSSWE
                     GTLGIITHKNPDPDAISSALALAVIAKNANPNRLICRIFYDGIIGHQANRTLVNLLEI
                     KMEKMDPAVLQECTYLALVDSPAPGVNNTLPSKTRVHIVIDHHE"
     gene            952..1025
                     /locus_tag="PK154_06740"
     tRNA            952..1025
                     /locus_tag="PK154_06740"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:986..988,aa:Lys,seq:ctt)
     gene            1452..2291
                     /locus_tag="PK154_06745"
     CDS             1452..2291
                     /locus_tag="PK154_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10795.1"
                     /translation="MQEADFLNNPEHPPDSPTGDVLFFSTRQKKVAISSPVKVLILDL
                     VKRGPTPLEIVVERSGKAKSTITKHVQDLEEAGLIVTHTNPDDNRRRILTLSADEIGR
                     LTAPESRMRPGIYYIPKLHSSFNGEEIAQFFRFLLVSFRLKAMAIGINIDPVLKQAGI
                     QIGEVLAPAVADESIEGVVKKIAQFWEIHHLGTVALRSTSPLSIEVRDCFECKDLPVT
                     GHEACPFDNGVITAIFSHHLKSPVTVVEEECYSKGNDRCLFVITRSSEHSSLVAGTKE
                     SGQ"
     gene            complement(2417..2599)
                     /locus_tag="PK154_06750"
     CDS             complement(2417..2599)
                     /locus_tag="PK154_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10796.1"
                     /translation="MNILVIDADRQAKQRRLRKVIDLFELYTIRYGNTVHLVPLDWPI
                     LFTLSEECDRSLMQRL"
     gene            2598..2825
                     /locus_tag="PK154_06755"
     CDS             2598..2825
                     /locus_tag="PK154_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10797.1"
                     /translation="MEIASFALKRIPLYGFSDVTKPEKVVGGHWGVTISSEIPKARWR
                     SVGGVISIYEISPAYAGERFLLVSDEINLSD"
     gene            3311..4150
                     /locus_tag="PK154_06760"
     CDS             3311..4150
                     /locus_tag="PK154_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPW10798.1"
                     /translation="MVLSLSRMQSYLLLLFAILLLGTLGVMFVEGLPVFDALYFVIVT
                     IATVGYGDISPSDVYGRAITILIIIAGVGTFVAVFAEIMENLISREERKSRRRKINMI
                     VGLFFSEFGNGMLRTLSSGDACIESIRKELLISSSWESADFERLKKNLEDHEYCIETD
                     AVNLVELKEHLSKKKEFLMLLLENPVIFEHDSFTDTLQAFFHLTEELTFREDPAHLSP
                     PDTAHLLNDINRGYRLLVFEWVTYMEHLKEHYPFLFSLAMRTNPFDPEAHAEIRDQEQ
                     RER"
     gene            4536..5168
                     /locus_tag="PK154_06765"
     CDS             4536..5168
                     /locus_tag="PK154_06765"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW10799.1"
                     /translation="MYCPRCGKETEQSGNFCQSCGTSFKDLPRRPFTRKRTGAIKTER
                     FSGLSGRFLGGLIDLFFLILFDLMVAAAIGIISWLLSRPDPVSESVRMLYQYYYHQPR
                     TNAAGQVVNAVIPPQILLSALIFLIIVPWIYSAYLESSRNQATLGKMAIRAAVTDLQG
                     NRITFARGSLRFFGKVLSVLTIFIGFIIIAFTAYKQGLHDKIAGTLVFRQ"
     gene            complement(5633..>6636)
                     /locus_tag="PK154_06770"
     CDS             complement(5633..>6636)
                     /locus_tag="PK154_06770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW10800.1"
                     /translation="PAVDAEKLRIARKTGERAVGLVKEGVAPRDIITTASLENAIRVD
                     MALGGSTNTVLHLMAIATEAGVPLDIDAFNRIGCRIPHLCHMMPAGPHSVLALHRSGG
                     IPAVLNALQRELEDAPTVSGNSIREIARKARIPAESIIQSVETPVNPAGGLRILKGTL
                     APTGAVVKSAAVSHEMWHHQGPARVFDGEKEAMEAILSRHVREGDVVVIRYEGPRGAP
                     GMPEMLSPTSALMGLGYTRVALVTDGRFSGGTRGPCIGHVSPEAAAGGPIGLVHDGDS
                     IEIDIRERRIDLHLSKAELKRRKAFFAPKQKKLAGILARYARIVGQADKGAVLERYD"
BASE COUNT         1650 a         1717 c         1570 g         1699 t
ORIGIN      
        1 cctcatggtg gtcgatcacg atatgcaccc gtgtttttga gggaagggta ttattcacac
       61 ccggggcggg agaatccaca agggcaaggt aagtacattc ctggagcacc gcgggatcca
      121 tcttctccat cttgatctca aggaggttga cgagggtccg gtttgcctgg tgcccgataa
      181 tcccatcata aaagatccgg catatcagcc ggttcgggtt ggcatttttc gcgatgacgg
      241 caagggcgag cgcacttgag atggcatcgg gatcgggatt tttatgggtg atgatcccga
      301 gagttccctc ccatgaagag aggagggaat aaaggcgctg ggagacgcga ttggcataca
      361 ccttgttgat ctggtagact gcagccttgg ccatcagctg gttctggttg aggacaaaat
      421 ctgccccggc ctcttccagg tttcgcgcaa caaggggatc tgtagcccgg gccattatcc
      481 gggaatgggg gtacgctacc ctgagcgctt taacagcagc aaggttggct tcgttatctc
      541 cagtgaggac aaacgcaatc tcaatcgcag gaatgcctga gaggaaatcc agggcggaga
      601 tgtcacggcg cacggcatcg atcctctggt ctctgagttc cctgacgcga tcctcatttt
      661 tatcaataac gagaacattc cccgtactgc tctgcaattc ctgcagtacg tggtatcccg
      721 tgctgccgca tcccagaacg gcatacaata cccgttccct gctcccattc tgcgccgaat
      781 ctgccataac agattagatg taaacccgac aggatataat ggttacaatt ttatgtgaat
      841 ctcaagggtt ttggaacacg ttacatgctc cgtttcccga tccgggcaag aaatcatgcg
      901 aactcgaaag gtttatcaat gaggcacaac caatggtata ggtcaaatca cgggcttgta
      961 gcttagttcg gaagagcgac ggactcttaa tccgtaggtc aagggttcaa atcccttcag
     1021 gcccgctgtt ttcccgattt tagcgtatct tttggggtat gatccacttc ctattctgtg
     1081 cagttgaaaa atccttgaat tctggagttc ccaccatctt tttcccaccc agatgttcaa
     1141 ttgacatatc tgtggattct ttacacattt cagtgctatt tccttatccg ggaataggtt
     1201 ctgctgcaga aacaaaaaac aggctacaat cacgagggag aatcatccag catcacagac
     1261 ggataaattc caaatggaga atgtattgga aaatctctat tctgcatgga tctggcagca
     1321 gatagataaa cgatacatta tggagcatct gcatcctcat tggccggata tatattaaaa
     1381 aagggctctg gtttcggtag attttataaa acactcattt taaataaggg tgaagcaaga
     1441 taataaaaga catgcaggaa gcggatttcc tgaacaaccc cgagcatcca ccggattcac
     1501 caactgggga tgttctcttt ttttcaacac gacagaaaaa ggtggcaatc agcagtccgg
     1561 taaaggtgct tattctcgat ctggtaaaaa gaggtccaac accactggag atcgtcgttg
     1621 aacgaagcgg gaaagccaag tcgaccatca caaaacatgt ccaggatctc gaagaggcag
     1681 gacttattgt cacccatacc aatccagacg ataacaggcg tcggattctt accctctctg
     1741 cagatgagat cggccgtctg acagcccctg aaagtcgtat gagaccagga atatactata
     1801 tcccaaaact tcattcttcc ttcaacggcg aggaaatcgc tcagttcttc cgtttccttc
     1861 ttgtcagctt ccgcctaaaa gcaatggcca tcggaatcaa catagatccg gtgctgaaac
     1921 aggctggcat ccaaataggg gaggtgcttg cccctgctgt ggcggatgag agcattgagg
     1981 gtgtcgtgaa gaagatcgcc caattctggg agatacatca cctgggcacc gtagctctcc
     2041 gcagtacatc gcctctttca atcgaggtga gggattgctt tgaatgcaag gatctcccgg
     2101 taaccgggca tgaagcctgc cccttcgaca atggcgtaat tacggcaatc ttctcccacc
     2161 atctgaagag cccggtgaca gtcgttgagg aagaatgtta ttcaaagggg aatgatcgat
     2221 gcctctttgt gatcacgcgc tcatcagagc acagttcatt ggtcgccggg acgaaagaat
     2281 caggacagta aactacctcc tgtagtgtct tcaagaggac tttttttggg gatattccgc
     2341 ttaacaataa tatcttcacc gtgtcatcgg taaaatggaa cgtatctccc ggtctccctg
     2401 tagtcgcact gtgagatcac aacctttgca tcaatgagcg gtcgcattct tcagagaggg
     2461 tgaagaggat cggccagtca aggggcacaa gatgcaccgt attaccgtat cggattgtgt
     2521 acagttcgaa aagatctatt actttcctca gtctcctttg cttcgcctgc cgatctgcat
     2581 cgattacaag aatgttcatg gaaattgcct catttgcgct gaagagaatt ccattgtatg
     2641 gattctcgga tgtgacaaaa ccagaaaaag tggtaggcgg tcattggggg gtgaccatct
     2701 ccagcgagat cccaaaagcg agatggagat ccgttggggg ggtgatctcc atttatgaga
     2761 tctcaccggc ctacgcagga gaaaggtttc tcttggttag cgatgagatt aatctatcag
     2821 attaatctta atcgcataat attagttatc tgctgaacat ttataatttt attgctctcg
     2881 aaccattagt gaataatgaa ttataatcct gatcgccagg tgaaacacaa tcaggagaac
     2941 gttttttcag gaaacacatc aatcgagaat cgtaggatcg gtcttaggag gtatgctatc
     3001 ccatggatca gatcatcatc cgaatgggtc gaaccgtgtc gcccactggt gagtgatgct
     3061 caataaaatc cctttcaaat acccatgaca gccccgcaaa aaccattatc tgatcctcct
     3121 cacagccttt cttaccgtca tttcatcagg atatcccagg aactccacca tttgcattgt
     3181 cttgtttcat caccatggca cgttctggtt tcattctcgt gagggggatg ccccgcttct
     3241 tcattcattt tcacccctcc ggcacctgtt cccaaggttt gatatcgctc aaggaagtcc
     3301 atgtactgat atggtactct ccctctcccg gatgcagtca tacctcctcc tgctcttcgc
     3361 catcctcctc cttggtacgc tcggggtgat gttcgtggag ggactcccgg tttttgacgc
     3421 actctatttc gtcattgtaa ctatcgcgac ggttggatat ggtgacatct ctccatcgga
     3481 tgtctatggg agagccatca cgatactgat catcattgcc ggggtgggta cattcgttgc
     3541 agtctttgct gaaatcatgg agaaccttat ctcgcgggaa gagaggaaat cccggcgaag
     3601 aaagatcaat atgatcgtag ggctcttttt ttctgagttt ggaaatggaa tgctccgaac
     3661 gctctcttct ggcgatgctt gcatcgaaag tatccggaag gagcttctca tctcatcatc
     3721 atgggaatct gcagattttg aacgattgaa gaagaacctt gaggaccatg aatactgcat
     3781 tgagacagat gcggtgaacc ttgttgaact gaaagaacat ctctccaaaa agaaagaatt
     3841 tcttatgctt cttcttgaaa atccggtaat attcgagcat gattctttta cggataccct
     3901 ccaggctttc ttccacctga cagaagagtt gacgttccgg gaagatccag ctcacctttc
     3961 ccctcctgat acagcccatc tcctcaatga tatcaaccga ggatacaggc tcctggtttt
     4021 tgaatgggtg acctacatgg aacacctcaa agaacattac ccgttcctgt tctcccttgc
     4081 gatgcgtacc aatcccttcg atccggaagc ccatgctgag attcgcgatc aggagcagag
     4141 agagagatga gatgcactcc ctctctttcc ctttcgttgg agattcgaaa cagagacatc
     4201 ctcctttgaa acacattctc ctctcttggt ctctcttaca ggtagccgac actcaccaca
     4261 tctgtcagtc ctgaacgatt tcgatactga acaggagtaa atgagaagag atccccccat
     4321 ttctcatcaa tagtcgcctc gtaaaggaat atccttgcag aacctccacg ttatccggga
     4381 ataccaattc tccaggtatc acaacgaaca cgcccaggtc tttcagaacg agaatacaca
     4441 ataaattcaa atatgacccg tcttcagact ccgggtcaac aagaatcgat acattgaagt
     4501 ggtgccctgc cgtcacttaa caaaggagga gactgatgta ctgtcccaga tgcggaaaag
     4561 agacagaaca gtcgggaaat ttctgccagt catgcggcac cagcttcaaa gaccttcccc
     4621 gcagaccttt tacgaggaaa cgaaccggag caataaaaac agagagattc tccggtctat
     4681 ccggccggtt tctcggtgga ctgatcgatc tcttctttct gatcctcttc gatctgatgg
     4741 tggcagcagc cattggtatc atctcatggc tcctgtcaag acctgacccg gtttcagaat
     4801 cagtgaggat gctgtaccag tattattacc atcagccaag aacgaatgct gccggacagg
     4861 tggtcaacgc agtcatcccc cctcagatcc tcctttctgc cctgatcttt ctcattatag
     4921 ttccctggat ctattctgct tatcttgaga gctcacgcaa ccaggcaaca ctcgggaaaa
     4981 tggcgatcag ggctgctgtc accgacctgc aggggaaccg cattaccttt gcccgcggat
     5041 cgctccgatt ttttggcaag gtgctctcag tcctgacgat attcatcgga ttcattatta
     5101 ttgcattcac tgcatataag cagggtctcc atgataaaat tgcaggaacc cttgttttca
     5161 ggcagtaacc atggtttccg ccttcattca tttcgtcagt ctgcctgacc gggggtatgc
     5221 tgatcttttc cttgtggcat cccgaatccc cgtagtagtt taatcgtatt tccctttcag
     5281 cccggcccat ccggtgtacc cgcagtaaat gcagccctct ccttcgcaat gacgacactt
     5341 tttggcagga gccttcacag gcacgcaccc tttcttatca cagtatatac acccgagacc
     5401 ctcgcaatga gaacaggtga cctcaacata agatactttt tcgaccatgg cttgcaggag
     5461 agctatggca gccgatgcac attaaacgtt attgaaagga gggatatggt ttcacctctg
     5521 ctttacattt aggaaccgaa gggactcgaa gaaaaggaaa aggactcgaa aaaaggtcaa
     5581 agagagaacg ggctcgtaat gccgaagttc cctgatgtca ggaacatgac gtttaatcgt
     5641 atctttcgag caccgctccc ttatcagcct ggcccacaat tctggcatag cgggcaagaa
     5701 tccctgccag ttttttttgt ttcggggcaa agaacgcctt gcgccgtttc agttcagctt
     5761 tcgatagatg cagatcaatt ctccgctccc gtatatcgat ctcgatacta tccccatcat
     5821 gcacgagacc gatgggccct cctgctgctg cttctggcga gacatgcccg atacagggac
     5881 cccgggtccc accagaaaac ctcccatcgg tcacgagagc aacacgggta taccccagcc
     5941 ccatcagcgc tgatgtcggg gagagcattt caggcatccc cggagctccg cgtggcccct
     6001 cgtagcgaat cacgaccaca tcaccttccc gtacatggcg ggagaggatt gcttccatcg
     6061 cctctttctc cccatcaaaa acccttgccg ggccctggtg gtgccacatc tcatggctga
     6121 ccgctgctga tttcactacg gctcccgtgg gtgcaagggt tcccttgaga atccgcagtc
     6181 ctcctgcggg attcaccggt gtttcgacag actgaatgat tgactcagcc ggaatcctgg
     6241 ctttgcgtgc tatctccctg atggaatttc cggatactgt cggagcatct tcaagctcgc
     6301 gctgaagagc attgagtact gccggtattc cccctgaacg atgcagggca aggaccgaat
     6361 gggggcctgc cggcatcatg tggcacaggt gggggatccg gcagccgatt cggttgaatg
     6421 catcaatatc aaggggaacc ccggcttcag tggcaatagc catgagatgg agaaccgtgt
     6481 tggtgcttcc cccaagcgcc atgtccaccc ggatggcatt ctccaggctt gcagtggtga
     6541 tgatatcacg aggggcaact ccctctttca caaggcctac cgcccgttct ccagtcttgc
     6601 gtgctattcg cagcttttcg gcgtctactg ccggtg
//
LOCUS       DAOOCO010000105         6615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_10879_length_6615_cov_5.521646, whole genome shotgun sequence.
ACCESSION   DAOOCO010000105 DAOOCO010000000
VERSION     DAOOCO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10879_length_6615_cov_5.521646"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK154_06775"
     CDS             complement(<1..1098)
                     /locus_tag="PK154_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPW10801.1"
                     /translation="MFGCNPPRNRVLLVLPLSGVVLFPRTRTKLNVEQPIGEHIAAEL
                     KEGSPVEVLALATAEDSDPTAESLYRAGSLLKIGGAEPADHGYLIEGVATEKLAVTSI
                     EERDGRFFATCTPFPDRLDLDEPTKKALLADIRAVIRDISSHFPGSGPFNRAIDEMDS
                     VDKVIGHVMLFAPLPIAAKQALFETESARERGRMFLELMQELGEQISFRVEMAQKVSE
                     KVNKSNREAMLREQLKLIQEELNEIEGGVPGDSGYRERIEQSKMPEEVRKKALSEARK
                     LEASGGQNHESHIIRNYLDLLLDLPWLTEEKTTVDIAEARTVLDANHTGLEKVKERIV
                     QHLAVMKLKHEKQGSILLLTGPPGTGKTSLGK"
     gene            complement(1126..1542)
                     /locus_tag="PK154_06780"
     CDS             complement(1126..1542)
                     /locus_tag="PK154_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPW10802.1"
                     /translation="MDAEERLLAAMERLIRLQQEISSAILSECGVPDITGKQIEYLKV
                     IDEQGDVTFTRLAEITRNSKPTVTEMINRFVGMECVYRERSSCDGRVLFIRLTEKGRR
                     IARADEASLARLVSRLEGSLDSDDLERLSRIIRNIR"
     gene            complement(1787..2563)
                     /locus_tag="PK154_06785"
     CDS             complement(1787..2563)
                     /locus_tag="PK154_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10803.1"
                     /translation="MQRPLLMLLLFLLCCGIAGSFWLLTAYPSILSLHGGTSGGAVVK
                     ETFTTGYLDPDRWEMTREGDAHQSLIEVVPVSPSDQDEYHLRLGMDTIGTMDDTVKYI
                     GIKSRGSINLRQAREISFDLDWNNQSNGCYLTAAFFLCPTETSRNPEREPEWLKIEYI
                     GVPPGKNARAIVAIKADDSVRFLYTEGWPEERSGRPVGYQRIVLIPDIEGITILENGS
                     FLYYIPQNDLHLESGYLYFQMSSHSNYPFREVYIDNIIVK"
     gene            complement(2638..5145)
                     /locus_tag="PK154_06790"
     CDS             complement(2638..5145)
                     /locus_tag="PK154_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HPW10804.1"
                     /translation="MKKATKFSLLCLVLLFSVLVVGIATAKNDENFEKYEKGDKEFKK
                     LEIGNMDVYWHQRRIDDAVVEGDFIVYQFDKKTEKLLKKEAKWRDDLPDHVTPEIKQK
                     QAESLVVGEVILSQLLFISPDSVEFPIKPTPKNPCWVVISEENDRRITTVIDAMDGGI
                     LGYGPSPPYEAYSLGGPDHGACAEYYYDPYALNAGNWFTTMGYSTEISDRPPEATVEG
                     HIDSSTTALFYELAHGGSYNFLNLNTGCPTPVAHHIFASNVETWIADRTKMPFTFIGS
                     CEGMCHTDDGSFSYEFRKGSTTDTVTVGYCHMDWTGDATLGETYSCSAPGGCWANAVA
                     WQDDLFNYLNQGDTAQTAFNKACADNPICGDGTTCVRFAGDPGLTLVPKVIRGSLPTA
                     NAGPDQTVEQTSLAGTSVSLDGTGSNDPDGAPLTYAWTWSGGSATGATPTVSLPLGAT
                     IITLVVDDGQGTDPDEDVRTDTVSVTVQDTTPPFLSVPLDVTVEQETIAGSAVSLTAT
                     APDICDASPEVASDAPAVFPLGTTTVTFTATDDSGNSASATMQVTVEDTTPPVIMVPP
                     DITVEQTSAAGTPVDPADVNATVTDICDASPDLTDDAPAVFPLGTTTVTFTATDDSGN
                     SASAMMQVTVVDTTPPVITVPPDVTVEQTSAAGTPVDPADVNATVTDICDASPDLTDD
                     APAVFPLGTTTVTFTAIDYTGNVAFATMEVTVEDTTPPEISVAVAPDTLWPPNHKMVN
                     IDASVTASDICDADPIIVLASIISDEPDDADGIGDGETTGDIQSANLGTEDYKFRLRA
                     ERAGEQDGRIYTITYQATDFSGNSATGSATVSVPLEM"
     gene            complement(6026..6166)
                     /locus_tag="PK154_06795"
     CDS             complement(6026..6166)
                     /locus_tag="PK154_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10805.1"
                     /translation="MPSTIALGCILTVVLTRLTAGLAITEPGAAVYGAEGAFEVDWVC
                     GR"
     gene            complement(6258..>6615)
                     /locus_tag="PK154_06800"
     CDS             complement(6258..>6615)
                     /locus_tag="PK154_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10806.1"
                     /translation="VTVVDTTPPTISVSVMPDSLWPPNHKMVDIQATVSANDICDASP
                     DVVLTSITSDEPDNGLGDGDKPNDIDITTLFTFKLRSERSGEYDGRVYTITYTATDDS
                     GNSASATDTVIVPLEK"
BASE COUNT         1484 a         1803 c         1698 g         1630 t
ORIGIN      
        1 cttaccgagg ctggtctttc cggtccccgg tggacccgtc agcaggagga tcgagccctg
       61 cttctcgtgt ttcaacttca tgaccgcaag gtgctggacg atccgctcct tcaccttttc
      121 aagccctgtg tggttggcat cgagcactgt gcgggcttct gcaatgtcga ccgtggtctt
      181 ctcctcagtc aaccagggaa ggtcgaggag caggtccagg taattcctga tgatatggct
      241 ctcatggttc tgccctccgg aggcctccag tttccgtgcc tcggaaagcg cttttttgcg
      301 gacctcttcg ggcatcttcg attgctcgat ccgctctctg tatcccgaat caccaggcac
      361 ccccccctcg atctcgttca actcttcctg gatcagcttc agctgctcac ggagcatcgc
      421 ctcgcggttt gatttgttca ccttctcgga gaccttctgt gccatctcca cccggaagct
      481 gatctgctca cccagttcct gcatcagttc aaggaacatc cgcccccgct cccgggccga
      541 ttccgtctca aacagggcct gcttagccgc gatcggcagc ggggcaaaga gcatgacatg
      601 accgatgacc ttgtcgaccg aatccatctc gtcaatagcc cggttgaagg gacccgatcc
      661 cggaaaatgg ctgctgatgt cacggatgac tgcccggata tcagcaagga gtgccttttt
      721 tgttggttca tcgaggtcca gccgatccgg gaagggggtg caggtggcaa agaaccggcc
      781 gtcccgctct tcgatgctgg tgacggcgag cttctcggtt gcgactcctt cgatgaggta
      841 gccatggtcg gcaggctccg ctcctccgat cttcaggagg cttccggcac ggtacaggga
      901 ttctgcagtg ggatccgaat cctcggcagt ggcaagtgcc agcacctcga ccggggatcc
      961 ctccttcagt tctgcagcga tatgttcacc gattggttgt tcgacgttca actttgtccg
     1021 ggtccgtgga aagagcacga ccccggagag gggtagcacg aggagcacgc ggttcctcgg
     1081 ggggttacat ccaaacattg gttctgttcc ttggtggaca ggggattatc tgatattcct
     1141 gatgatccgg gaaagacgct ccagatcatc gctgtcgaga gatccttcca gccgggagac
     1201 gaggcgtgca agcgaagcct cgtctgcccg ggcaatcctg cggcctttct cggtcaggcg
     1261 gataaaaagg acacgtccgt cacaggagga ccgttcgcgg tacacgcact ccatgcccac
     1321 gaaccggttg atcatctcgg tgaccgtggg cttcgagttc cgggtgatct cggcaagccg
     1381 ggtgaaggtc acgtcgccct gttcatcgat gaccttcagg tattcgatct gctttcccgt
     1441 gatatcggga actccgcatt cggagaggat ggcagaagag atctcctgct gcagcctgat
     1501 cagccgttcc atcgcggcca gcagccgctc ctccgcatcc atgacctctg cctgtaatta
     1561 gtttggtctt acctaataat aagtttcaga tggaggcaag gctacgggag gcgtactcat
     1621 ctccctggga gttgtcacca cctcctggta tgaatgcaag gaggggggaa tgatcgaatg
     1681 gtcccgtcag aaaagggtac atgacggaat acgggggccg tggtgaatcc ccccaaccaa
     1741 tcctaaaatt catattcctt acctttaagc aatctgaaaa aaaacttcat ttcacaatta
     1801 tgttatcgat atagacctcg cggaaggggt aattgctgtg gctgctcatc tggaaataga
     1861 gatatcctga ttccagatgc agatcatttt gggggatata atagagaaag gatccatttt
     1921 caaggatggt tatgccttcg atgtccggaa tcagaacgat tcgctggtat ccgacaggtc
     1981 tccccgatcg ctcttccggc catccttcgg tatacaaaaa cctgacgcta tcatcagcct
     2041 tgattgcaac gattgcacgt gcgttttttc ccggggggac accgatatat tcaattttga
     2101 gccattccgg ctctcgttcg ggattcctgc tcgtttctgt gggacacaaa aagaaggcag
     2161 cggtcaggta acagccgttg ctctggttgt tccagtcgag atcgaaggag atctcacgtg
     2221 cctgacgaag attaatcgac cctctactct taatcccgat atacttgacc gtatcgtcca
     2281 ttgtcccgat ggtgtccatt cccaatctca aatggtactc gtcctggtct gaaggagaga
     2341 cgggaaccac ttcaatgaga ctctgatggg catctccttc tctggtcatc tcccaccggt
     2401 caggatccag ataaccggta gtgaaggtct ccttcacgac agctcctcct gaagttcccc
     2461 catgaagaga cagaatggag ggatacgcgg taaggagcca gaagctaccg gcaataccgc
     2521 agcacaacag gaacaggagc aacataagga gaggtcgttg catggtagat cctcctcaca
     2581 ggtactcgta tcatgaacgt gtactatcaa aaaattttgg agattatatc tgcaagatta
     2641 catctcaagt ggtaccgaaa cggtcgcact gccggtcgca ctgttgcccg agaaatcagt
     2701 tgcctgatac gtgatggtgt atatcctgcc atcctgctcc ccggcccgtt cagccctcag
     2761 tctgaattta taatcctctg ttccaaggtt tgcagactga atatccccgg tggtttcgcc
     2821 atccccgata ccatcagcat cgtcgggttc gtcactgatg atacttgcca ggacgataat
     2881 cggatctgca tcacagatgt cgcttgcggt gacactcgca tcgatattca ccattttatg
     2941 attcggcggc cagagggtat caggggctac cgctaccgag atctcaggcg gggtcgtatc
     3001 ctccactgtc acctccatcg ttgcaaaagc aacgttacct gtataatcga tcgcggtgaa
     3061 ggtcactgtg gttgtgccga gcgggaacac tgccggtgca tcgtcggtca gatcagggct
     3121 ggcatcgcag atgtcggtga cagttgcatt cacgtctgcc ggatcgaccg gagtgccagc
     3181 tgcactcgtc tgctcaacag tgacgtcggg tggaacggtg atgactggcg gagttgtatc
     3241 aaccaccgtc acctgcatca ttgcactggc actgtttccc gaatcatcgg tcgcggtgaa
     3301 ggtcaccgtg gttgtaccga gcgggaacac tgccggtgca tcgtcggtca gatcagggct
     3361 ggcatcgcag atgtcggtga cagttgcatt cacgtctgcc ggatcgaccg gagtgccagc
     3421 tgcgctcgtc tgctcaacag tgatatctgg tggaaccata atgactggcg gggttgtatc
     3481 ctccaccgtc acctgcatcg ttgcagaggc actgtttccc gaatcatcgg tcgcggtgaa
     3541 ggtcaccgtg gttgtgccga gcgggaacac tgccggtgca tcgctcgcaa cttccgggct
     3601 ggcatcgcag atgtcggggg cagtcgctgt cagtgataca gctgaacctg caatagtttc
     3661 ctgctcaacc gttacatcca atggaaccga gaggaaagga ggtgttgtat cctgaaccgt
     3721 gacggatacc gtgtcggttc tcacatcctc atcaggatcg gtcccctgtc cgtcatcgac
     3781 caccagggtt atgatagtcg cacccaacgg aagcgagacc gtgggagtgg cccctgtcgc
     3841 tgagccacct gaccaggtcc aggcatacgt caatggtgca ccatcaggat catttgaacc
     3901 cgttccatcg agtgagaccg aagtcccggc aaggcttgtc tgttcgaccg tctggtcggg
     3961 acctgcgttt gccgtaggca ggctgccccg gatcaccttg ggaaccaggg tcaggcctgg
     4021 gtcgccagcg aaacggacgc aggtagtccc gtccccacag atgggattat ctgcacacgc
     4081 tttattaaaa gcagtctggg cggtatcccc ctggttcagg tagttgaaca gatcatcctg
     4141 ccatgctact gcattagccc aacaaccccc gggtgcggag caggaatagg tctcacccag
     4201 agtcgcgtcg ccagtccagt ccatgtgaca gtaccctacc gtgaccgtat cggtggtgga
     4261 ccctttcctg aactcgtagg agaaggatcc gtcatccgtg tggcacatcc cttcgcagct
     4321 tccaatgaag gtaaatggca tcttggtcct gtctgcgatc catgtctcaa catttgaggc
     4381 aaagatatga tgagcaacag gagtaggaca tccggtatta aggttcagga aattatagct
     4441 ccccccatgc gccagttcat agaacaacgc ggtggttgaa ctgtcaatat gcccctccac
     4501 ggttgcttcc ggaggtctgt cacttatctc ggtggaatac cccatggtgg taaaccagtt
     4561 tcctgcattc aatgcataag gatcatagta atattctgca caggcaccat ggtcgggccc
     4621 accgagtgaa tacgcttcgt agggtggcga aggaccatag cccaaaatac cgccatccat
     4681 tgcatcaatc acggtggtga tccttcgatc attctcctcg ctgattacca cccagcaggg
     4741 atttttgggc gttggcttga tgggaaattc taccgagtct ggtgatatga acaggagctg
     4801 ggagaggata acttcgccca cgaccagcga ttcagcctgc ttctgcttga tttcaggggt
     4861 tacatgatcc ggcagatcat cccgccactt tgcctccttc ttcagaagct tctcagtctt
     4921 cttgtcgaac tggtagacaa tgaagtcccc ttcgacaaca gcatcatcta tcctcctctg
     4981 gtgccagtag acatccatgt tgccaatctc tagtttcttg aattctttat cccctttctc
     5041 atatttctca aaattttcgt cgtttttggc cgtagcaatt ccaacgacga gtacactgaa
     5101 aagaagtaca agacagagga gagagaattt tgttgccttt ttcatactag ttcctccatt
     5161 tcctcaacat acgatcgatg tttgtaccct gaaagggctg cgaattcgcg tctgcccgcc
     5221 tcatcggaaa aaagaaccgg tctccctggt atcgctccaa ccttgctcag ggcagacaca
     5281 ctaaccaata cggatttata tgtgtttgca aagcagataa cattacaaat gtgttaataa
     5341 atatagaggg tattttatag attagatacg aattaaaata ttcccagata taacaattgg
     5401 tcacgttctg ctcattcagg cagacggtct ccagaaaact ccggcaggca tggacctcgt
     5461 acgagaatat ccctttcatc accgcagggt cctcgttcat aatattccgg gtttccggag
     5521 ggccatggag ggaatcccgg actccacaga gctcgctgcc gattgctttg gttaattcag
     5581 tcgagtccaa cggataccac cttcccacaa ccgatgatga ctggtcaggc atatcgtatc
     5641 ccgatgaatt tcacagtatc gtctctcgtt cagatggtgc gcatacccaa cctcaatgaa
     5701 tgcagatcac cgttgccaac gtctttaccg ggcatctcgt caacctttgt tgtgatatcg
     5761 ccctcccagg tcagagtcca cattgtggta tcgagaagac tggattcgaa gatgtcatgc
     5821 aatatcatca ataaaaacat accggcatgg atgtgcccat ttctccgcag gcatattatc
     5881 aataaaaaaa acctatccca cttcatcaag aagtgcgatt gatccatatc aggtgttgat
     5941 actggactct ccggaggtcg gaatcggata catgtagagc catgttcctt ccaatgagtt
     6001 attgagaatc tcagattgga ccctctcatc tgccacaaac ccagtcgacc tcaaacgctc
     6061 cttctgcacc atagaccgcg gctcctggtt cggttatcgc cagtccggcc gtcagacggg
     6121 tgagaactac ggtgagaata cagccaagag caatagtgga gggcatatca tgcttccttt
     6181 catgtcgtaa tcccttatat caccttaaaa gatttttgag tgggatatgt tcccatctca
     6241 ccggaagata tctcgggcta tttttccaat ggcacgataa cagtgtcggt cgcactggca
     6301 ctgttccccg agtcatcagt tgccgtgtag gtgatagtat agacccggcc atcatattca
     6361 cctgaccgtt ctgaacgcag cttgaaggtg aatagagtcg taatgtcgat atcatttggt
     6421 ttatctccat cgccaagccc attatccggt tcatcgctgg tgatgctggt gagcaccacg
     6481 tccggtgaag catcgcatat atcattcgct gacacggtcg cctggatatc caccatcttg
     6541 tggtttggcg gccacaggga atcgggcatg acagagacgc ttatggtcgg tggcgtagta
     6601 tcgacaacgg tcacg
//
LOCUS       DAOOCO010000106         6423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11297_length_6423_cov_5.634736, whole genome shotgun sequence.
ACCESSION   DAOOCO010000106 DAOOCO010000000
VERSION     DAOOCO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_6423_cov_5.634736"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK154_06805"
     CDS             complement(<1..480)
                     /locus_tag="PK154_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW10807.1"
                     /translation="MDLKDYLEDTATQVDKLVYRYFGETCGELGEASSHLLLAGGKRL
                     RPVMLILSADAIRKGSSIDLMPAALALELTHSFTLIHDDIMDGDVVRRGVPTVHTKWN
                     MPTAILAGDVLFASAFEFIAMTDAPDNAKVRAISMLGRTCVQICEGQHMDMSFESRDD
                     "
     gene            complement(500..1837)
                     /locus_tag="PK154_06810"
     CDS             complement(500..1837)
                     /locus_tag="PK154_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HPW10808.1"
                     /translation="MDIEIIAVGGYDEVGRNMTAVRCGKEIVILDMGLRLDQVMIHEE
                     AEVENLHSLDLIQMKAIPDDTMMNTVEGSVKAIVCSHGHLDHIGAIPKLAHRYNAPII
                     GTPYTVELIRQQIAGEQKFGVNNKLFALKHGQKYTLSQHLTLEFVRMQHSIIDTATVV
                     LHTPKGAIVYACDYKLDRTPVIGDPPDFARLRQIGKEGVLALIVESTYIDNRGRAPSE
                     RIARDLVRDTITSYEDDKNAIIVSTFSSHIARVKTIAECAHEIGRKPVLLGRSMERYS
                     STAEQMKLVAFPDTMSMFGNRRTVDRTLRRMVKMGKEKFLPIITGHQGEPGSILTRIA
                     LGDTPYKVEKGDKVIFSAKIIPNPMNIGQRYMVEAHLNMAGARIFPDLHVSGHAYRED
                     HYEFVHMLNPQHVIPAHGHIRMTSGYAEFTEEMGYTLHNDVHIMSNGMRVKLS"
     gene            complement(1851..2915)
                     /gene="fni"
                     /locus_tag="PK154_06815"
     CDS             complement(1851..2915)
                     /gene="fni"
                     /locus_tag="PK154_06815"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPW10809.1"
                     /translation="MEQRRGTSGRKLDHLQICLERNIERGDPGFSDIRLVHNALPECD
                     LDFLDTRTEFLGYTFKSPLFIAGMTGGHPGTKEVNRLLARTAAEQGIGMGVGSQRAAL
                     ENPDLEDTFSVVREVAPKAFLAANIGAVQLRDHGIEWAERAVSMIDADAIAIHLNFLQ
                     EAIQPEGDHDARGCLDAITELCREFRVPVMVKETGSGISAGTARLCWEAGVKAIDVGG
                     WGGTSWAAVEGLRTGTDSGEGKYQRRPASEVFEEWGIPTVISLMEVAGTGGPVIATGG
                     IRSGLDMAKAITLGAALCGIALPLLKPAMKGENELKRTVTDFCDELRVAMFLSGARNP
                     EELGERRPYITGRTRQMMMK"
     gene            complement(2921..3676)
                     /locus_tag="PK154_06820"
     CDS             complement(2921..3676)
                     /locus_tag="PK154_06820"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HPW10810.1"
                     /translation="MPEPVLRKLGGSVLTEKGGPGRIRKENLRMICQEISVHSRDPLL
                     IIHGAGSCGHPEAHRFRLMDGLTDHNREGVYVTHQAVSKLNSIVVAALRNEGIEAIGM
                     HPLDSCYAEEGRIVSMECRHIREMLAKGIVPVLHGDVVMDGMRGATVVSGDQLVSYLG
                     RALRFRRIGLATDVPGVLAGDTVVQLLTPQNVNQVLIGSSRHTDVTGGMEGKIRELLE
                     LARSGTPSEIFHVEQTGAFLQGKPHGGTVVRGG"
     gene            complement(3669..4538)
                     /gene="mvk"
                     /locus_tag="PK154_06825"
     CDS             complement(3669..4538)
                     /gene="mvk"
                     /locus_tag="PK154_06825"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HPW10811.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGIAMAIKPRVFVTVRKSRMAH
                     HAKSPYIDSCFDEMGVRGSVYVNSQLPSSSGLGSSAAVTVATLAAINDEFGKKFKAEE
                     IADMAFAIEKKVQKGRASPTDTTVSTFGGLVFITGTSRRRLPPQQFHLVVGNSLVQHS
                     TVKMVELVAELNRTNPRICGPVFDAIGEVSLAAMHHMSDPQKLGDLMNMNHALLEVLG
                     VGHPQISRLVLAARTAGGYGAKLTGAGGGGCMIAICPKHLKSRIVGAIESCEARAFST
                     SIDTEGVRKEKDA"
     gene            complement(4541..5338)
                     /gene="amrB"
                     /locus_tag="PK154_06830"
     CDS             complement(4541..5338)
                     /gene="amrB"
                     /locus_tag="PK154_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPW10812.1"
                     /translation="MKTRPAPLAGQWYPKEPYHYEQFLEGAFRKCRGRGIARGIIVPH
                     AGYLYSGEVAACAYAQFDPGFSGTFITIGPRHHVPLMTTTTPLPWETPFGLVECDREL
                     VTAFHLPINEFADAEYQENSLEMQMPFIKYRFPRARIAPVLMGDQSLDSAHRLADVIL
                     AAVRETGRDVRIVASSDLSHFHSDKVARQKDRQAIEAILKLDLQELYTRIGNGTAEAC
                     GLGPIATMCLVCQALGAGTTEEISYATSGEVTKDTRSVVGYAAIAVI"
     gene            complement(5390..5998)
                     /gene="rpsB"
                     /locus_tag="PK154_06835"
     CDS             complement(5390..5998)
                     /gene="rpsB"
                     /locus_tag="PK154_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW10813.1"
                     /translation="MTENEIEIELNEPLVPVEEYLAAGIHIGTQQKSKDMKRFIYRVR
                     GDGLYILDIRETDERIKTAARFLSQYEAPKILVVTSRQYGQYPAKKFAETIGGTAATG
                     RFIPGMLTNQNLNGYMEPEVVVVTDPIGDSQAIKEAVQCGIPIVALCDTNNMTSYVDL
                     VIPTNNKGRKALSMIYYLLTREILRIRGIATSLTLEDFETEL"
     gene            complement(5995..>6423)
                     /locus_tag="PK154_06840"
     CDS             complement(5995..>6423)
                     /locus_tag="PK154_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPW10814.1"
                     /translation="SYISELVDRYDLIYVEDPLHEEDFSGFSDLTSQVGDRCLICGDD
                     LFVTNVSRIVEGIEQGSANCVLIKPNQVGTLTDTFEAVHLAHSNGMDTVMSHRSGETT
                     DATIAHLATAFQCIFLKTGVVGGERTAKLNELIRIEEQIA"
BASE COUNT         1404 a         1870 c         1576 g         1573 t
ORIGIN      
        1 atcgtccctt gattcgaatg acatatccat atgctggcct tcacagatct ggacacaggt
       61 ccggccaagc atcgagatag cccgtacctt ggcattgtca ggcgcgtcag tcatcgcaat
      121 gaattcaaag gcgctcgcaa agagcacatc cccggccaga attgcggtag gcatattcca
      181 ttttgtatgt acggtaggga cacctctgcg taccacatcc ccgtccatga tatcatcatg
      241 gatgagagtg aaactatggg tgagctccag agcaagggcg gcgggcatca ggtcgattga
      301 actccctttc cggatggcat cggcagaaag aatgagcatg accggacgca accgtttgcc
      361 tccagccagg aggagatggg aacttgcctc cccaagttcc ccacaggtct ctccaaaata
      421 gcggtagacg agcttgtcga cttgtgttgc agtgtcctca agataatcct tcagatccat
      481 cgtgatagcc cttccttgat cagctcagtt ttacccgcat gccattcgac atgatatgca
      541 catcgttatg gagggtatat cccatctcct cagtaaattc cgcataaccg gatgtcatcc
      601 tgatatgtcc gtgtgccggg atgacatgct gggggttcag catatgaaca aattcgtaat
      661 ggtcctcccg gtacgcatgg ccgctgacgt ggagatcggg aaagatgcgt gcccctgcca
      721 tgttcaggtg tgcttctacc atgtaccgct ggccgatgtt catcggattg gggattatct
      781 tcgctgagaa gatcaccttg tcacccttct caactttata aggggtatcg cccagggcta
      841 tccgggtaag gattgagccg ggctctccct ggtgaccggt gataatgggg aggaatttct
      901 cctttcccat cttgaccatc ctcctgagag tccggtcgac ggtgcgcctg ttgccaaaca
      961 tgctcatcgt atccgggaat gccaccagtt tcatctgctc ggccgtggag ctgtaccgtt
     1021 ccatcgatcg gccgagaagc accggtttcc tgcctatctc gtgtgcgcat tcggcaatgg
     1081 tcttcaccct ggcgatatgc gatgagaatg tgctgacgat aatcgcgttc ttgtcatcct
     1141 cgtagctcgt gatcgtatca cggaccagat cccgggcgat acgttcgctc ggtgccctcc
     1201 ctctgttgtc aatatacgtg ctctctacga tgagggcaag cactccctcc tttccgatct
     1261 gtctcagtcg ggcgaaatcc ggcggatccc caatgacagg agtccggtcc agcttgtaat
     1321 cacatgcgta gacgatggct cctttcggcg tgtgcaaaac aacggtagca gtatcgatga
     1381 tactatgctg catcctcaca aactcgaggg tgaggtgctg ggacagggta tacttctgcc
     1441 cgtgtttcag ggcaaacagc ttgttgttca ccccgaattt ctgttcacct gctatctgct
     1501 gccgtatcag ctcaaccgta taaggggtcc cgatgatcgg ggcattgtac cggtgagcca
     1561 gcttcggaat tgccccgatg tgatccaggt ggccgtgaga gcagacgatc gccttaacgc
     1621 tcccttcgac cgtattcatc atcgtgtcat caggaattgc cttcatctgg ataagatcca
     1681 gggaatgcag gttctccact tcagcctctt cgtgaatcat cacctggtca aggcgaagac
     1741 ccatatccaa aatgacaatc tctttgccgc agcggacggc ggtcatattt ctcccgacct
     1801 cgtcataccc gccaacggcg attatctcaa tatccattat ttacctccat ttatttcatc
     1861 atcatctgtc gagtcctgcc ggttatgtaa ggccgcctct cccctaattc ttcaggattt
     1921 cgtgccccgg agaggaacat agccacccgc agttcatcgc aaaaatcagt cacggttctt
     1981 ttcagttcgt tctcgccctt cattgcaggt ttcagcaggg ggagcgcaat accacacagg
     2041 gcggcaccga gagtgatcgc cttcgccata tcaaggccgc tcctgattcc accggtcgcg
     2101 atgacgggac ctccggtccc tgcaacctcc atgaggctga tgacggtggg aatcccccac
     2161 tcttcaaaaa cttccgatgc aggccgacgc tgatacttcc cctcgccgga gtctgtacct
     2221 gtgcggagtc cttcgactgc agcccatgaa gtcccgcccc atccgccgac atcaattgct
     2281 ttcaccccgg cttcccagca gaggcgggca gttccggcgg atatcccgct ccccgtctcc
     2341 ttcaccatca ccgggacgcg gaattctcta cacagctcgg tgattgcatc aaggcaccct
     2401 ctggcatcat ggtcaccttc cggctggatc gcttcctgca gaaaattgag atggatggca
     2461 atcgcatcgg catcgatcat cgataccgcc cgctccgccc attcaatacc atgatccctc
     2521 agctgaacag cgccgatatt cgcagccaga aaggcttttg gggccacctc ccggactacg
     2581 gaaaaggtgt cctcaagatc aggattctca agagctgccc gctgggaacc aactcccatg
     2641 ccaataccct gctctgcagc agttctggca aggagtcggt tcacttcctt tgtccccggg
     2701 tgcccgccag tcatccctgc gataaacaag ggggatttga acgtgtatcc cagaaattcg
     2761 gtcctggtgt cgagaaaatc aagatcacat tcgggaagtg cattgtgaac gagccggatg
     2821 tccgaaaagc caggatctcc ccgttcgatg ttccgttcaa ggcagatctg gagatggtcc
     2881 aacttccttc ccgaagtccc ccgtcgttgt tccaccacgt ttatccacct ctcaccacgg
     2941 tacccccatg gggctttccc tgcaggaacg cccctgtctg ttcaacatgg aagatctcgg
     3001 agggagtgcc gcttcgggca agttccagca gctcccggat ctttccttcc ataccgccgg
     3061 ttacatccgt gtgacgggat gaaccaatca ggacctgatt cacattctgg ggagtgagca
     3121 actggaccac cgtatctcct gccagcactc ccggaacatc ggtggcaagc cctatccgtc
     3181 tgaaacggag tgcccggccc agatacgaga ccagctggtc accggaaacg accgtagctc
     3241 ctctcatccc gtccatcacg acatcgccgt ggaggaccgg gacgatcccc tttgcgagca
     3301 tctcacggat atgccggcat tccatggaaa caatacggcc ctcttcggca tagcaggagt
     3361 ccagcgggtg catgcctatc gcctcgatcc cctcgttccg gagggctgcc accacaatgc
     3421 tgttcaattt cgatactgcc tgatgagtaa catatacccc ttcgcggttg tgatcggtca
     3481 ggccatccat gagccggaag cggtgggctt cgggatgccc gcaggagcct gctccgtgaa
     3541 tgatcagcaa tggatcccgg gaatgaactg atatttcctg gcatatcatg cgaaggttct
     3601 ccttccggat cctgccggga ccacccttct ctgtcaatac actcccccca agtttccgca
     3661 aaacaggttc aggcatcctt ctccttcctt accccttcgg tatcgatgct cgtggagaaa
     3721 gcccgtgctt cacacgattc gatagccccg acaatacgac tcttcagatg cttggggcag
     3781 atggcgatca tgcatcctcc accgcctgca ccggttagtt tcgccccgta tccgcctgct
     3841 gtacgggctg caagtaccag gcgggagatc tggggatgac cgacgccgag cacctccagg
     3901 agtgcatggt tcatgttcat cagatccccc agcttctggg ggtcagacat gtggtgcatg
     3961 gcagcaaggg atacctctcc aattgcatca aatactggtc cacagatcct gggattcgtc
     4021 ctgttcaact cagcaacaag ttcaaccatc tttaccgtgc tatgctgaac cagggaattc
     4081 cctaccacaa ggtggaactg ctggggggga agtctcctcc gtgatgtacc ggtgatgaat
     4141 acaaggcccc caaaggtcga aacggtggtg tccgtagggc tcgcccgccc tttctgcacc
     4201 ttcttttcga tagcaaacgc catatccgca atctcttctg ccttgaattt tttcccgaat
     4261 tcatcgttta ttgcagccaa tgttgcaacg gttactgctg ccgatgatcc gagcccggat
     4321 gaactcggga gctgggagtt gacatagaca ctccccctga cccccatctc atcaaagcag
     4381 ctgtcaatat agggggactt ggcatgatgg gccatccggc tctttcgcac ggtaacaaag
     4441 accctgggct tgatcgccat ggcgatcccg ggtttcccgt acaccacagc atgctccccg
     4501 aacaggaaga ctttgccagg cgcactccac gtggccaccc tcagatcacc gctatggctg
     4561 cgtatcccac gacactccga gtgtctttcg tgacctcgcc acttgttgca taactgatct
     4621 cttctgtagt accggctcca agagcttgac aaaccagaca catcgtagca atcggaccaa
     4681 ggccgcatgc ttcagcagtt ccatttccaa ttctggtata gagctcctgc aggtcaagtt
     4741 tcagaattgc ttcaatagcc tgtctgtctt tttgtcgtgc taccttatct gaatggaaat
     4801 gagagagatc cgatgatgcc acaattctca catctcggcc cgtttctcga acagctgcaa
     4861 gaatgacatc cgcaagtcga tgagcactgt caagactttg atctcccata agaaccgggg
     4921 ctattcttgc acggggaaac cgatatttaa tgaacggcat ctgcatttca agagagtttt
     4981 cctgatattc agcatcagcg aattcattga tcggcaggtg aaatgcggtc acaagctctc
     5041 gatcacactc gacaagacca aacggtgttt cccagggaag aggggtggtg gtcgtcatca
     5101 atggcacgtg gtgccgaggt cctattgtga tgaaggtccc gctgaaacct gggtcgaact
     5161 gtgcataggc acatgcagca acctcccctg aatagagata ccctgcatgg ggtacaatga
     5221 tccccctcgc aattcccctt cccctacatt tccgaaacgc accttcaagg aattgctcat
     5281 agtgatacgg ttctttcgga taccattggc cggcaagagg agccggtcgc gtcttcatcg
     5341 aatcaacctt ctggttctca ttatatataa gggagattgc atccacacct tatagttcgg
     5401 tctcgaaatc ctctaaggtt aaggacgttg caattccacg aatcctcaaa atttctcggg
     5461 tgagcaggta atagatcatc gacagagctt ttcttccctt gttgttggtg gggatgacaa
     5521 ggtccacata acttgtcatg ttgttggtat cgcacaatgc tacgataggg ataccgcatt
     5581 gcaccgcttc cttgattgcc tgtgaatccc cgatgggatc ggtcacgacg acaacctccg
     5641 gttccatata cccgttcaga ttctgattgg tgagcatccc cgggataaac cgtcctgttg
     5701 ctgccgttcc cccgatggtc tccgcaaact tcttggcagg gtactggcca tactggcgcg
     5761 aagtaacaac aaggatcttc ggtgcttcat actggctcag gaaccgtgca gcggttttaa
     5821 tacgttcatc ggtctccctg atatcaagga tgtagagccc atccccgcgg acacggtaga
     5881 taaaccgctt catatccttg cttttctgct gggttccgat gtgtattcct gctgcaaggt
     5941 attcctccac cgggacaagt ggctcattca gttcaatttc tatctcgttt tctgtcatgc
     6001 tatctgttcc tcgattcgta tgagttcgtt caattttgca gttctctccc cgccaacaac
     6061 acctgttttc aagaaaatgc actggaaggc ggttgccagg tgggcgattg tcgcatcagt
     6121 cgtctcaccc gaacggtgac tcatgacggt atccatgccg ttgctatggg cgagatggac
     6181 tgcttcaaag gtatcggtaa gagtccctac ctggtttggt ttgatcagca cgcagtttgc
     6241 cgatccctgt tctatccctt caacgattcg ggagacgttg gttacgaaga gatcatcacc
     6301 acagatcagg cacctgtcgc ccacctggga ggtcaggtca gaaaatcctg agaaatcctc
     6361 ctcatgcagg ggatcttcca catagatcag gtcatagcga tccactagtt cactgatata
     6421 aga
//
LOCUS       DAOOCO010000107         6359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11463_length_6359_cov_5.633725, whole genome shotgun sequence.
ACCESSION   DAOOCO010000107 DAOOCO010000000
VERSION     DAOOCO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11463_length_6359_cov_5.633725"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PK154_06845"
     CDS             <1..353
                     /locus_tag="PK154_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPW10815.1"
                     /translation="NSVGEEENIPLEARLADLEANNIEKIHNKGVIVDKTRVLVSSIN
                     WNDNSPGFNREIGVIIEHAGVGDYFTRVFEDDWMASALYTGKKGPDWIKIGIALAVLF
                     TLAGLAYIKKKTVF"
     gene            complement(410..715)
                     /locus_tag="PK154_06850"
     CDS             complement(410..715)
                     /locus_tag="PK154_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW10816.1"
                     /translation="MKNAGCDIIVCDIMVRKYLPAMRAEMVTRLVQREGITQSDAAKM
                     LGVSRAAVSQYMSRKRGDSGVEISHELDSLIDQWALSVTSSDTGITLCDICRCAMKR"
     gene            complement(712..942)
                     /locus_tag="PK154_06855"
     CDS             complement(712..942)
                     /locus_tag="PK154_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW10817.1"
                     /translation="MPQVNLPPSSRAVLHLLDEGGAMTHKEIVRKSKLAPRTVRYALK
                     KLKENQLIIEKFNFRDARQIIYQNREPQQVPA"
     gene            1012..1383
                     /locus_tag="PK154_06860"
     CDS             1012..1383
                     /locus_tag="PK154_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10818.1"
                     /translation="MFVRQERIAFLLLCFVSVSIIATTFILAGIDKSILASDYTPRSD
                     EGSLVHLEGEIKEVHTTKTGGHILATINGTAVFIPATIAGEVVLLEGDYVSLYGLVQT
                     YQGEREVVLNSLSDITILPER"
     gene            complement(1465..2040)
                     /locus_tag="PK154_06865"
     CDS             complement(1465..2040)
                     /locus_tag="PK154_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW10819.1"
                     /translation="MAADTEEPLDIKFIDINLRRRNIQAYIDLLKSPHLDTRWKAAEQ
                     LGIIGDQTAVLPLIESLKDPFVEVSWLAAKSLGSIGDARAVEPLLQALNSTEPWLRAG
                     AALGLGKLRDRRAVEPLIALLNDKKKQVRKHAALALGLIGDERALASLEKAAQADMDE
                     NVKTAAAHSARMIRISSESGREGKETGDSEE"
     gene            complement(2142..2978)
                     /gene="nadC"
                     /locus_tag="PK154_06870"
     CDS             complement(2142..2978)
                     /gene="nadC"
                     /locus_tag="PK154_06870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPW10820.1"
                     /translation="MHSLEHLLSFIREDAPYGDITSEAVIGDIRCHAFISTREPGLVA
                     GLEEAAALFSYFGATILNQSHDGCEVTAGTTIMSLEGPARGILLVERTALNIIGRMSG
                     VANRTRLLASLLASTGATTRIAATRKTSPGMRLLDKKAVLLGGGDPHRINLSDGILIK
                     DNHLALVSLESAIMRAKQRSLYRKIEVEISTPANAVAAAKAGADILLLDNMTPDLVRE
                     TLALCTKHGLRDHLIIEVSGNIDETNLLLYAIPGVDRISAGTLTHSVRNFDVSLEITH
                     KL"
     gene            complement(3156..3914)
                     /gene="nadX"
                     /locus_tag="PK154_06875"
     CDS             complement(3156..3914)
                     /gene="nadX"
                     /locus_tag="PK154_06875"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HPW10821.1"
                     /translation="MLSIGLLGCGNIGHIIAEYQGNWKVVALYDKVHERACELALVSG
                     GTPYENFEDFINAPFDLVVEAASVKAVRAYAETILLHEKHMVVMSVGALSDPLFRSQM
                     IDIARRQKRKIHIPSGAITGLDNIKVGQIAPITRLLLRTTKNPASLGLPASERTLIFE
                     GKANECIKEFPRNVNVSVALSLAAGREVDVELYVDPAVDRNIHEIIVEGVFGDIYIRV
                     NNLPSPDNPATSYLAALSILSLLTNLEEPLVVGT"
     gene            4085..5317
                     /locus_tag="PK154_06880"
     CDS             4085..5317
                     /locus_tag="PK154_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW10822.1"
                     /translation="MSHGEYFSNLAHKDVCILTFGCTYNHGDSRKLETVLISRGCRIV
                     PEPEKADALVVNTCTVVGATERKMIRILKKYCDMPLYVTGCMMVVQREEICAVCNPGF
                     IHPDAITEEYNRIPHRTPHETGIVQIGKGCRGTCTYCITRYARGSPVNHTMEEILDDI
                     RTCHENGAFEIRLCAQDCSAWRGEDHETLADLLSRTGDLQGSFLIRIGMMNPATLLPI
                     LDPVIESFSSSRLFRFIHLPVQSGSDRILSRMGRGYTRDEFTSIVNEFRKKIPDISIA
                     TDIITGFPGEDEEDFQASIDVISAVRPVKVNITRYSPRPHTMIADARAPTDYVRKIRS
                     RKVQAHAEVLYYRQNRSLLGRTIGVTVTEHIRPGSVLCRSSNYTGVIVESDLPVGAKV
                     RVCLVEDRMYFFSGELVS"
     gene            5442..5981
                     /locus_tag="PK154_06885"
     CDS             5442..5981
                     /locus_tag="PK154_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10823.1"
                     /translation="MDEIEAGFQKLTERIKEREKERESLADEVKTHEVALFGRLAGIA
                     APLIPSIGILMLQRGKQDTKGELYDTLFHKKKMIVLGKTDPAGHRPDNMAKKVDDQFC
                     VLSEDGKFYELMFSFDGFIVDSYANPITAKDALDRYGYEPMYMLYQALHDYLKGQEDL
                     VAALKLVLAFVFPVSAAKK"
     gene            complement(5984..6322)
                     /locus_tag="PK154_06890"
     CDS             complement(5984..6322)
                     /locus_tag="PK154_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10824.1"
                     /translation="MEDSRMHPDVNAMLKNGAKAIELKNRGNAAAFAIHVAIIPLDIE
                     FDVPLLKEDEKYLYAFEKMINEVKVAVSFENENKERFSKTYLLSATGKSDEDLLKPAF
                     PLFKWRKEDD"
BASE COUNT         1593 a         1497 c         1613 g         1656 t
ORIGIN      
        1 tcaacagcgt cggagaagag gagaacatac ccctggaagc gcgccttgcg gatttggagg
       61 caaataatat tgaaaagatc cataacaaag gggtaatcgt tgacaaaacg agggtattgg
      121 tcagcagcat caactggaat gacaactcac ccggtttcaa tcgggaaata ggggtgataa
      181 ttgaacatgc gggagttggg gattatttta cccgggtctt tgaggatgac tggatggcat
      241 cagcattata cacaggaaaa aaaggacctg actggatcaa gatcgggatt gctctcgcag
      301 ttctctttac cctcgcgggc ttggcatata tcaagaagaa gaccgtattt tagctcatat
      361 atgatataga ctgagaacag cgttggactt tttggaggct ctacctgaat tatcgtttca
      421 tcgcacatcg gcatatgtca cagagtgtga tcccggtgtc tgagctggtt acggacaggg
      481 cccattgatc aataagcgaa tcgagttcat gtgagatctc aacccctgaa tctcctctct
      541 ttctgctcat atactgcgat actgccgcac ggctgacacc aagcattttt gcagcatcgc
      601 tctgcgtgat cccctctcgt tgcacgagcc gtgttaccat ctcagccctc atggcaggaa
      661 ggtactttcg taccattata tcacagacaa tgatatcaca gccggcattc ttcatgcagg
      721 cacctgttgc ggctcgcgat tctgatagat aatctggcgg gcatcgcgga aattgaactt
      781 ttcgatgatc aactgattct ccttcaactt cttcagggca tagcgcacag tcctgggcgc
      841 aagtttgctc ttcctgacaa tttccttatg ggtcatcgcg ccaccttcat cgaggagatg
      901 caggacagcc cgggatgagg ggggaaggtt tacctgtggc atggataagt gttaacgctg
      961 ttaacatatc aaactgtcga ttataaatct gattatgacc tggatccata tatgtttgtc
     1021 cggcaggagc gtattgcctt tctgcttctc tgttttgttt cggtgtcgat cattgcaacc
     1081 acattcattc tcgccggtat tgacaaatcc attctggcat cagactatac tccccgatca
     1141 gatgagggtt cattggtgca tcttgaggga gaaataaaag aagtccatac cacaaaaacc
     1201 ggagggcata tccttgctac cattaatgga acggcggtct tcatccctgc caccattgca
     1261 ggggaggtag tgctgcttga aggggactac gtctccctgt atggcctggt tcagacatac
     1321 cagggagaga gggaagttgt gctgaattcc ctcagtgata tcacgatact gcctgagaga
     1381 tgatcgggct gatcaggatc tcaaccaagc taatgaaaac gttttcccat gaaatctgaa
     1441 ttcagaaaaa tttctgcggc agatttattc ttctgaatca ccggtttctt tcccttccct
     1501 gccagattca gaggatatcc gaatcatcct tgcagagtgt gctgctgctg ttttcacgtt
     1561 ttcatccatg tcagcctgtg cagccttttc aagagatgcg agtgcccgtt catccccaat
     1621 gaggcctaat gcaagggcag catgtttccg gacctgtttc tttttatcat taaggagcgc
     1681 tatcaatggc tccaccgccc ttctgtcacg aagcttgcca aggcccagtg cagcaccagc
     1741 ccgtaaccag ggttctgtcg agttcagcgc ctggagcagt ggttcaaccg cccgtgcatc
     1801 cccaatagag cccagcgatt ttgcagccag ccatgagact tcgacaaagg gatccttgag
     1861 tgactcgatt aatggcagaa ctgctgtctg gtcgccgata atccccagct gctcagccgc
     1921 tttccaccgc gtgtcaagat gcgggctttt cagaagatcg atgtatgcct gaatatttcg
     1981 cctgcgaaga ttgatatcaa tgaatttgat gtcaagaggt tcttctgtat ccgcagccat
     2041 caggcaacac ccgctcacgg gggatttgtg acaggctcta ttaattgttt ggttttgaga
     2101 ttttcttgag tgactgttaa aatccgtcca agagttcttt tctataattt atgagtaatt
     2161 tcaaggctga cgtcgaaatt tcggacagaa tgggtgaggg ttccagcgct tatccggtcc
     2221 accccgggaa tggcgtacaa gaggagattt gtctcatcaa tgtttcccga aacctcaatg
     2281 ataaggtggt cccggaggcc atgtttggta cataatgcca gcgtctccct gacaaggtca
     2341 ggggtcatat tgtccaggag aaggatatca gcacccgcct tggcagcagc aacggcattg
     2401 gcaggggtgg agatctccac ctcgattttc cgatacagag aacgctgctt tgcccgcatt
     2461 atggcactct caagggaaac gagagcgaga tggttatcct tgatgaggat accatctgaa
     2521 aggttgatgc ggtgtggatc ccctccgccc agcagtaccg cttttttgtc cagaagccgc
     2581 ataccaggcg aggtttttct ggtagcggcg atacgggtcg ttgctcccgt ggatgcaagg
     2641 agggaggcga gtaatcgggt ccggtttgca acaccactca tccttcctat gatgttcaaa
     2701 gcggttctct caacgagaag aatccctctg gcagggcctt ccagcgacat gatagtggtg
     2761 ccggcagtaa cttcgcatcc gtcgtgagat tgattgagta tggttgcacc gaaataggaa
     2821 aagagggctg cagcctcttc gagtcctgca accaatcctg gttcacgggt ggagatgaac
     2881 gcatggcacc tgatatcccc aattactgct tcactcgtaa tatccccgta gggggcgtcc
     2941 tcgcggataa aggaaagaag gtgttcaaga gagtgcatgg ggttgtccgt ccgaagagag
     3001 aaatggtgat gttgtttccc ggtacaatcc gttcgaggcc gttttgtgaa ttcgcatacc
     3061 tgcctcttca tcgtgtgagg aaatgctggg gactccaatt gctgcacatc tggggatttc
     3121 agaggcagca ttcccttttg ggaatttttg tggattcatg ttccaaccac cagcggctct
     3181 tcgagattgg taaggagtga gagaatggag agcgccgcta ggtaactggt agccggatta
     3241 tcagggcttg gcagattgtt cacacggata tatatatcgc caaagactcc ttccacgatt
     3301 atctcatgga tgttcctgtc aacggcagga tccacataga gctccacgtc cacctctcgt
     3361 ccggctgcaa ggctgagtgc cactgagaca tttacattcc gggggaattc cttgatgcat
     3421 tcattagctt ttccttcaaa tatcaatgtc cgctctgatg cagggaggcc aagagatgcc
     3481 ggattctttg ttgtacggag caggagccgt gtaatgggtg cgatctgacc gacttttatg
     3541 ttatccaggc ctgtaatcgc gccgcttggg atatggatct ttctcttttg cctccgggct
     3601 atgtcgatca tctgtgacct gaaaaggggg tctgataatg cacccacact catgacaacc
     3661 atatgctttt catgtaaaag gatggtctct gcataggccc ttactgcttt taccgaggcc
     3721 gcctcaacga ccaggtcgaa aggagcattg ataaaatcct cgaaattttc atacggtgtc
     3781 cccccagaaa caagtgcgag ttcacacgcc cgttcatgga ctttgtcata aagggcgacg
     3841 accttccagt ttccctggta ctctgcaata atgtgcccga tatttccaca accgaggagc
     3901 ccgatgctca acatcactac aacatacaca acgctatcgg ttaagcattt tgcaaaccag
     3961 gcgtgagggg ggggaggatg ttctctcgga tctcgacggc agcgcatgcc cccccctgga
     4021 tatatatggg gttagacaga aaccacacac gggtataaag tctccagccg gatctgatcc
     4081 aaaaatgagt cacggagaat acttttcgaa ccttgcacat aaggacgtct gtatcctcac
     4141 gtttgggtgt acttacaatc atggagacag caggaaactg gaaacggttc tgatctctcg
     4201 aggctgcagg attgtgccgg aaccggaaaa agccgatgca ctggtagtga atacatgcac
     4261 ggtagttggt gcaacagagc ggaaaatgat cagaatactg aaaaaatatt gtgatatgcc
     4321 cctctacgtc acaggctgta tgatggttgt gcagagagaa gagatctgcg cagtctgtaa
     4381 tcctggattt atccatcctg atgcaataac tgaagaatac aatcggattc cacatcgtac
     4441 ccctcatgaa acagggatag tccagattgg gaaaggatgt cgcggaacct gtacgtactg
     4501 catcacccgg tatgcaaggg gttctcctgt caatcatacg atggaggaaa tactggatga
     4561 tatccgaaca tgccatgaaa atggggcatt cgagattcgg ctctgtgccc aggactgcag
     4621 cgcatggagg ggggaggacc atgaaacctt ggctgacctt ctttctagaa ccggagatct
     4681 tcagggatcg tttctcatcc gcatcggtat gatgaatccc gcgaccctcc ttccgattct
     4741 cgatcctgtc attgaatcgt tttcgagttc acgactattc cgttttatcc accttcccgt
     4801 ccagtccggg tcagaccgga ttctttcgag gatgggcaga gggtatactc gtgatgagtt
     4861 tacttcaatc gtgaatgagt tccgaaaaaa aattcccgat atttcgatag ctacggacat
     4921 cattacggga tttcctgggg aggatgaaga ggacttccag gcctcgattg acgtaatctc
     4981 tgcggtgcgg cctgtcaaag tgaacattac ccgttattcg ccccgccccc acactatgat
     5041 tgccgatgca agagcgccta ctgattatgt aaggaagatc agatcacgaa aggtacaggc
     5101 tcatgctgaa gtgctgtatt atcgtcagaa tcgttccctg cttggcagaa ccatcggtgt
     5161 tactgtgacg gaacacatca gacccgggag cgtactctgc agaagcagca attacaccgg
     5221 cgttattgtt gagagcgatc ttccggtagg ggcgaaggtc agggtatgtc ttgttgagga
     5281 ccggatgtac ttcttctcag gggaattggt atcatgaggg ggaacctcat gcaaggaata
     5341 gtgtatagca caatagagtt tatactgatt catttacgac ggaaggatta aacatgaggc
     5401 acaccaattc cagtcattca ccataggatg aggaatgtac gatggatgag attgaagctg
     5461 gatttcaaaa attgactgaa aggatcaaag agagggaaaa agagcgggag tccctggcag
     5521 acgaggtaaa aacgcatgaa gttgcgctct ttggtaggct ggcagggatt gctgccccgc
     5581 tgattcccag tatagggatc ctgatgttac aacgtggcaa gcaggacaca aagggggagc
     5641 tgtatgatac cctgttccat aaaaagaaga tgattgtcct gggaaaaacc gatccggccg
     5701 ggcatcgtcc tgataatatg gctaaaaaag tggatgatca gttctgcgtc ctttcagaag
     5761 atggaaaatt ttatgaatta atgttctctt ttgacggatt tattgttgac tcttatgcaa
     5821 atccgataac cgcgaaagac gcacttgatc ggtatggata cgaaccgatg tacatgctct
     5881 atcaggccct ccatgattac ctcaaaggcc aggaagatct ggttgcggca ctgaaactgg
     5941 tcctggcatt cgttttcccc gtatctgctg ccaagaaata agattaatca tcctcttttc
     6001 tccatttgaa gagtggaaat gccggtttta acaagtcctc gtcactcttg ccggttgccg
     6061 ataagagata tgttttcgaa aagcgctctt tattttcatt ctcgaaggag actgccactt
     6121 ttacctcatt gatcatcttt tcgaaggcgt agagatattt ctcatcttct ttcaggagag
     6181 ggacgtcgaa ttcaatatcc aacggaatga tcgcgacgtg aatcgcaaag gctgcggcat
     6241 ttcccctgtt tttcaactct attgcctttg ccccgttctt taacattgcg ttcacatccg
     6301 gatgcatccg ggagtcttcc atgatccaga cggacatcag gagggcgatg aagatgatg
//
LOCUS       DAOOCO010000108         6301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11635_length_6301_cov_5.156260, whole genome shotgun sequence.
ACCESSION   DAOOCO010000108 DAOOCO010000000
VERSION     DAOOCO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11635_length_6301_cov_5.156260"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..634
                     /locus_tag="PK154_06895"
     CDS             170..634
                     /locus_tag="PK154_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10825.1"
                     /translation="MPNHLAESMCRIRELTLGAVIISIIISAGCSTLKPDTPSYNNDK
                     NPIVGLWFSESPDSVTYFIFYENLTFTARSRSGSDVPDYRYQYFGTWDVSGGNQYTTT
                     GQHIGYGEVTALAIWRDLTILYDPASDTFTIPVYDNQEFHRISKIPEEPGIV"
     gene            complement(658..933)
                     /locus_tag="PK154_06900"
     CDS             complement(658..933)
                     /locus_tag="PK154_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair domain-containing protein"
                     /protein_id="HPW10826.1"
                     /translation="MRKSREHLLRMYHDPGYRFADVGICFINRGAPNDVSCVRGDQIL
                     RLDPYYIEVASEKGTTPIPYHRLIRICYHGEVVWDRGKQGKSGSSSP"
     gene            1037..2272
                     /gene="mmp10"
                     /locus_tag="PK154_06905"
     CDS             1037..2272
                     /gene="mmp10"
                     /locus_tag="PK154_06905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314610.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HPW10827.1"
                     /translation="MAYLTVDIGGKPGVNCRGFCEYCYFKHVKEVPPFGCRHCPPYRV
                     GCDYCSRSVREYYQGWKSLRQVGEETLATLQMMTGDVERVTISGGGDPSCYPEFTDLI
                     ELLAGMEVPLHIGYTSGKGFDDVGIADFLIDHGLAEVSFTVFSVDPAKRKRYMHDPTP
                     DVSLKILERLCGEIEVYGAAVVLPGINDGKALEETCQWLDDRGAKGLILMRFANRTDQ
                     GLILGNSPVIKGQPVQSPQEFQEMVTDLAKQFNMKISGTPLWDPAIGSPFAIRNEPDL
                     LDKLPLLQRSATVITGSIAAPAIQAILDARGGGVNVIGTRKEIACLITADDLNALDLA
                     EIESLVVIPGRAFVHDQEAERILTADGNDRTVLRGPEMLTADAETSMGMTREGVLLIE
                     LEGFSGLIAVINRYGQDSP"
     gene            2459..2917
                     /locus_tag="PK154_06910"
     CDS             2459..2917
                     /locus_tag="PK154_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW10828.1"
                     /translation="MARIHARRRGDSGSVRPHRSEAPSWSNTDVKAIEKVIVDLKKEG
                     YSSSRIGLVLRDQYGVPDVKLVMGKRIEQIIKDNGLQSEIPEDLRNLMMKALGLRKHL
                     GENRNDLHNKRQLQLTESKVRRLVKYYVSSGRLPSDWSYKPETAEILLSR"
     gene            2923..4140
                     /locus_tag="PK154_06915"
     CDS             2923..4140
                     /locus_tag="PK154_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPW10829.1"
                     /translation="MSLEEAVSRVAQELERQDFVEVYAHHDADGIAAGSILCSALFRR
                     GIRFRLRILDHISPASLSPGIPTVLCDLGSGLEDLPEGVMVIDHHIPRFEGDLHVNPR
                     LHGIDADQELSSSGAAYFVARELGDNRDLAGLVMLGIIGDDQELSGMNREIFNEAMGE
                     SVISMNNGCRLVGRDLHEKLLCSINPYLPRISGDEMAVADLIETSSREGKIEGDRLLS
                     LIVLRISLFVPARTLNSLYGERFSLDREIISDAHTFTAIVDACGKCGQGGLAASLCLR
                     SAEGTDEAWEAAQSHRWRVLQSLTALAGRDEKQSFYEVEERSVASDVADVLARDTVQE
                     RPVFVTARDNDLCHVSARAPGTSDLNLGDLIHEAAVRCGGFGGGHRTRAGATIACSRI
                     GEFRDTLARMGVA"
     gene            4137..4361
                     /locus_tag="PK154_06920"
     CDS             4137..4361
                     /locus_tag="PK154_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HPW10830.1"
                     /translation="MTDIEGIIITRHRHAGCVAGALRPDNLSAMSTIACGDEVRTRIT
                     GRKIRSLIASTDDYLMNCAIAEDLCRYASD"
     gene            4340..5869
                     /locus_tag="PK154_06925"
     CDS             4340..5869
                     /locus_tag="PK154_06925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPW10831.1"
                     /translation="MQVRFRLDAELRFSAPFSKEAEESCRALIDEANRVLFMKGVPKG
                     VDRSEVGQITRSEFSGNTLTLTIESGAYTRAHDALFRFRKQVAPSLGKHRLGIREIVV
                     TDYSIELEGAFPENFRTPELPFIKKAIHSGRVLFVNLDVSASDLENRIPDRLVRLFEE
                     KIEAAGYGGKGEHWELIYESGTKALHFEGDPTAEMIQRGWIKHALSRGQWIHGPQSTQ
                     LFRAFEQIVIEEIIGPMGFTEMIFPKLVPWEVWMKSGHAKGVYPEIYYVCTPKTRDAA
                     YWEDIGDYFKVTGEVWVDEIRNRIDGPIGGLCYAQCPSFWPFLQGETLANDCLPIRIF
                     DRSGTSHRYESGGIHGIERVDEFHRIEILWLGTAEQTVETADRLHDAYTHVFEDLLEL
                     EWRCAKVTPWFMAQEGMIGPDNGCGCGGRIGTTDYEALLPYSNSWLEFQNVSINGDKY
                     PKGFNVKIQSGADCWSGCSGIGLERWTAAFLAQKGLDIGNWPDIVAKKVGEPKNLFKF
                     L"
     gene            5879..>6301
                     /locus_tag="PK154_06930"
     CDS             5879..>6301
                     /locus_tag="PK154_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619240.1"
                     /note="the function for this protein is unknown; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HPW10832.1"
                     /translation="MAKKKQVGRRVEGWKAKSWYKIYTPESLGKAYIGDTIAGDPESV
                     VGRIIQTTLGEILNDYSRQNVKMKLRIANVAADAAYTEFVGHELTRDYLRSLVKRRTT
                     RIDSHVPVLTKDGKMVDLSVTCYTLTRANLSQTHAIRGL"
BASE COUNT         1492 a         1628 c         1827 g         1354 t
ORIGIN      
        1 ccccagccgt gatttgaaca cggggcctgc tgattacaag tcagccgctc tgccagctaa
       61 gccactgggg cgccatatac catacgcgtt atatcataaa aaaagtggtg aagaagggaa
      121 catactcctg agactgatgc gggatgagca ggaagggata tcataggggt tgccaaatca
      181 ccttgctgag agcatgtgtc gtatcaggga actgactctt ggtgcagtca tcatcagcat
      241 aataatcagt gccggttgca gcaccctcaa gccagacacc ccctcttaca ataatgacaa
      301 aaatcccatt gtggggctct ggttctccga gtccccggat tcagtgacct attttatctt
      361 ttacgaaaat ttgacgttca cggcccggtc ccggagcgga tcggacgtgc cggactatcg
      421 ttaccagtac tttggaacat gggacgtttc gggaggcaac cagtacacca caacaggcca
      481 gcatatcggg tacggagaag tgacagctct tgctatctgg cgtgatttga ccattctcta
      541 tgatcctgca agtgatacat tcaccattcc ggtctacgat aaccaggaat tccatcggat
      601 atcaaaaatc ccggaagagc cgggaatagt ctaatagggt atccctatac cttcccgcta
      661 tggtgagcta ctgccggatt tgccctgttt tccccggtcc cataccacct cgccatggta
      721 acagatcctg atgagccggt ggtacggaat cggagttgtt cccttctcgg aggctacctc
      781 gatgtaatag ggatcaaggc ggagaatctg gtctcctctc acgcaactca catcattcgg
      841 tgctccgcgg ttgataaaac agatcccgac atcggcaaac cggtacccgg gatcatgata
      901 catccggagg agatgctccc tgcttttccg catgctgttt cctcgttatg cagttccatc
      961 tcaaaatagt tcacccatac ccccggtccc aaccgctatc catggattaa tccgaaaaga
     1021 gggcggatag ttattgatgg cgtatctcac ggtggatatt ggcgggaaac ccggagtcaa
     1081 ctgcaggggg ttctgcgaat actgttattt taagcatgtg aaggaagtcc ccccctttgg
     1141 atgccgccac tgtcccccct atcgggtggg atgtgattac tgcagccgga gcgtgcgtga
     1201 atactaccag ggatggaaaa gtctgaggca ggtgggagaa gagacgcttg ccacgcttca
     1261 gatgatgacc ggcgatgtcg aaagggtgac catcagcggc ggcggggacc cgagctgcta
     1321 cccggaattt accgatctga tcgaactcct tgcaggcatg gaggtcccgc tccacatcgg
     1381 atataccagc gggaaagggt ttgatgatgt cgggatcgcc gatttcctca tcgaccatgg
     1441 gctcgccgaa gtctcgttca cggtcttttc tgtcgatccg gcaaaacgga agcggtacat
     1501 gcacgatcca acccccgatg tctcgttgaa gatactcgag cgactctgcg gcgagatcga
     1561 ggtgtacggg gcggcggtgg tccttccggg aatcaatgat ggcaaggcac tcgaagagac
     1621 ctgccagtgg ctcgatgatc ggggagcaaa aggactgatc cttatgcgtt ttgcgaacag
     1681 gaccgatcag ggcctcatcc tcgggaattc tccggtgata aaggggcagc cggtccagtc
     1741 cccccaggag ttccaggaga tggtgaccga tcttgcgaaa caattcaaca tgaagatttc
     1801 agggacaccc ctctgggatc cggcaatcgg ctcgccattt gccataagaa acgagcctga
     1861 cctcctcgac aaacttcctc tcctccagcg gagcgcgacg gtcatcaccg ggagtattgc
     1921 cgcaccggcc atccaggcta ttctcgatgc acggggcgga ggggtcaacg tcattgggac
     1981 aaggaaagag attgcctgcc tcatcactgc cgatgatctc aacgcgctcg accttgcaga
     2041 gatagagagc ctcgttgtga tcccgggacg ggcatttgtc catgaccagg aggccgagcg
     2101 aatcctgacc gctgatggaa acgaccgcac agtgctccgc ggcccagaaa tgctgactgc
     2161 agatgcagaa accagcatgg gtatgaccag ggaaggagtc ctcctgatag agcttgaagg
     2221 attttccggg ctgattgcag tgatcaaccg gtacgggcag gacagcccct gatctgttcg
     2281 atatgcttat tatctgcccg catgaatgat aagcaggcac aactaatgtg ctggcatatg
     2341 taaatccgac gtgtggtaac aacacggcct cgctttttca gaggcttttg ggattacaga
     2401 agagtcagca gaggctgctc tttttccgga tttacatggt gccatctgga ggtgaaccat
     2461 ggcacgtata cacgcacgcc ggagaggtga ttcaggatcg gtccgcccac atcggagcga
     2521 agccccctcc tggtcaaata ccgacgtgaa agcaatcgag aaggttattg ttgacctaaa
     2581 aaaagaaggg tactcgagca gcaggatcgg ccttgttctc cgtgaccagt atggagtccc
     2641 tgacgtcaaa ctggtgatgg gaaaacggat cgagcagatt atcaaagata acgggctgca
     2701 gtcagagatt ccagaggatc tccggaacct gatgatgaag gcgcttggcc ttcgaaaaca
     2761 tctcggggag aatagaaacg acctgcacaa caagcggcag ctgcaactga ccgaatcaaa
     2821 agtacgccgc ctggtgaagt actacgtatc cagcggacga cttcccagcg actggagcta
     2881 caagccggaa accgcggaga tcctgctctc gcgctgatcc ccatgtccct cgaagaagcc
     2941 gtatccaggg ttgctcagga gctcgagcgg caggactttg tcgaggtcta tgcacaccat
     3001 gacgcggacg ggattgctgc cgggtccatc ctgtgttctg cactgttcag aagggggatc
     3061 cggttccggc tcagaattct ggatcatatc agccctgcgt ccctctctcc cgggatcccg
     3121 accgtgcttt gtgacctcgg ttcgggactc gaggaccttc ctgaaggtgt gatggtgata
     3181 gaccatcaca ttccccggtt tgaaggtgac ctccatgtca atccaaggct tcatggaatc
     3241 gacgctgacc aggaactctc atcctcaggg gctgcctact ttgtcgcaag ggagcttggg
     3301 gacaatcggg accttgccgg ccttgtcatg cttggaatca tcggtgatga ccaggagctg
     3361 tcagggatga accgggaaat tttcaacgaa gcgatgggag aatcagtcat cagcatgaac
     3421 aatggatgcc ggctggtggg aagagatctc catgagaagc tgctctgcag catcaatccc
     3481 taccttcccc gaatcagcgg ggatgagatg gctgtcgctg atcttatcga gacttcttcg
     3541 agggaaggga aaatcgaggg ggataggttg ctctccctca tcgttctgcg gatcagcctg
     3601 tttgtccctg caaggacact gaattccctt tacggagaac ggttttccct tgaccgtgag
     3661 atcatcagcg acgcccatac cttcacggcc attgtggatg cgtgcgggaa atgcggccag
     3721 ggcggacttg cggcatcact ctgtctccga tctgctgaag ggactgatga ggcatgggaa
     3781 gcagcacaaa gccatcgatg gcgtgtcctt caatcgctga ctgcgcttgc cggacgagac
     3841 gagaagcaga gtttctatga agtggaggaa cgatcagtgg ccagtgatgt ggcagatgtg
     3901 cttgcgaggg acacggtcca ggaacgtcca gtatttgtca cagcgcgtga caatgatctc
     3961 tgtcatgtct cggcccgtgc accgggaaca tcggacctga atctcggcga tctgatccat
     4021 gaagcggcag tacggtgcgg cggttttggc ggtggccacc ggacgagggc tggggctacg
     4081 attgcctgtt cgcggatagg cgagttccgt gatactcttg caaggatggg ggtggcatga
     4141 cggatataga ggggatcatt atcacccggc accgtcatgc aggatgtgtg gccggggctt
     4201 tgcgcccaga caatctctct gccatgagca cgattgcctg cggcgatgaa gtcaggacga
     4261 ggataactgg caggaaaatt cgttccctca tcgcatcaac tgacgactat cttatgaatt
     4321 gtgcaattgc ggaggatcta tgcaggtacg cttccgacta gacgcagagc tccggttcag
     4381 cgcccccttc tcaaaggagg ctgaggagtc ctgccgggca ctgattgatg aagcaaacag
     4441 ggtgctgttc atgaagggtg tcccgaaagg ggtggatcgc agcgaagtgg gccagatcac
     4501 gcgatccgag ttttcgggaa ataccctcac tcttactatt gagagcgggg catacaccag
     4561 ggcccacgat gcactcttcc ggttcaggaa gcaggttgcc ccctcactcg ggaagcaccg
     4621 gctcggtatc cgcgagatcg tagtaaccga ttattcgatt gagctggagg gagcattccc
     4681 ggagaacttc cggacaccgg aacttccgtt catcaagaag gcaatccatt ccggacgggt
     4741 gctctttgtc aatctcgacg tctccgcttc ggatctcgag aatcgtatcc ctgatcggct
     4801 ggtcaggctt ttcgaagaga agatcgaggc tgcgggctat ggaggaaagg gagagcactg
     4861 ggagctcatc tatgagagtg gaacaaaagc cctccatttc gagggtgatc caaccgcaga
     4921 gatgatccag cggggctgga tcaagcatgc actctcccgc gggcagtgga tccatggacc
     4981 gcagtcaacc cagctcttcc gggcattcga acagatcgtc atcgaagaga tcatcgggcc
     5041 tatgggattt accgagatga tcttcccgaa actggtgccc tgggaagtct ggatgaagtc
     5101 cgggcacgcg aaaggggttt atcctgagat ctactatgta tgcaccccta aaacgcgtga
     5161 tgctgcctac tgggaggata tcggtgatta cttcaaggtc accggtgaag tctgggtaga
     5221 cgagatcaga aaccggattg acgggccaat tggtggtctc tgctacgccc agtgcccttc
     5281 attctggccg ttcctgcagg gagagaccct ggcaaacgac tgcctcccca tccggatctt
     5341 tgaccggagc ggaacgtccc accgctacga gagcgggggt attcacggga tagagcgggt
     5401 cgacgagttc caccggatcg agatcctctg gctcggtact gcagagcaga cggtggagac
     5461 tgcagaccgg ctgcatgacg cctataccca tgtattcgag gatctcctcg agctggaatg
     5521 gcggtgtgcc aaggtgactc cctggttcat ggcgcaggaa gggatgatcg gtcccgacaa
     5581 cggctgcgga tgcggcgggc ggattgggac cacggattac gaggcgctgc ttccttacag
     5641 taacagctgg ctcgaattcc agaatgtcag catcaacggt gacaaatacc cgaagggatt
     5701 caatgtaaaa atccagagcg gggctgactg ctggtctggc tgctccggta tcggccttga
     5761 gcggtggaca gcagcatttc ttgcccagaa agggcttgat atcgggaact ggccggatat
     5821 cgtggcgaaa aaggttggag aacctaaaaa tcttttcaaa tttctctgat gtgatagtat
     5881 ggcaaagaaa aaacaggttg gacgaagagt tgaaggctgg aaagccaaga gctggtataa
     5941 gatatatacc cccgaaagcc tcgggaaggc atacattggg gataccattg caggtgaccc
     6001 cgagagcgta gttggcagga tcattcagac tacccttggt gagatactga atgattactc
     6061 caggcagaac gtgaagatga aattgcgaat cgcgaatgtg gcagcagatg cggcatacac
     6121 cgagtttgta gggcatgaac tgacccgtga ttacctccgt tccctggtaa agagaaggac
     6181 aacccggatc gacagccatg tgccggttct taccaaggac gggaagatgg tggatctgtc
     6241 ggtcacctgt tacactctca cgagggcaaa cctgagccag acccatgcaa tccgggggct
     6301 t
//
LOCUS       DAOOCO010000109         6252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11757_length_6252_cov_4.747781, whole genome shotgun sequence.
ACCESSION   DAOOCO010000109 DAOOCO010000000
VERSION     DAOOCO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_6252_cov_4.747781"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK154_06935"
     CDS             <1..436
                     /locus_tag="PK154_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HPW10833.1"
                     /translation="AAFVRRSARILALPITESGSREIAARSRGTPRIANRLLRRVGDY
                     ALVRGDGRIDEDLADQALTMLGVDLNGLDETDRRILRVIVEDFGGGPVGIKTIAISIG
                     EDVRTIEEVYEPYLIQSGFIKRTPQGREATPAARSHIQPAWW"
     gene            490..945
                     /locus_tag="PK154_06940"
     CDS             490..945
                     /locus_tag="PK154_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPW10834.1"
                     /translation="MIPFTGLELSPGKAAYLKYLSLQGGSVKISGIAGYFEVDPSTVT
                     RTITDLTREGYVEHEPYGRVILTDFGRKYGAFLVRRHRILGLVLSHYGFSEEEACTEA
                     SRMECYLSREAVNKICRALGHPTRGFCGEICHDPLCLSGTFHGLHENVS"
     gene            968..1897
                     /locus_tag="PK154_06945"
     CDS             968..1897
                     /locus_tag="PK154_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPW10835.1"
                     /translation="MIPRSGVMALALVLLVLAAGCNGVARNDPGEQISVAVTIPPQEE
                     MVREIGGERVEVVVMVPPGSDPHTYEPEPALVARAAGADLYVTIGTGLLPIEDTLVSR
                     LTAMNPGLVVADSSTGIPPLSPDQGGETSLVQGEDLGRETGQANPSPDPHIWLSLRNA
                     VIMSENTRDALIKVDPANENVYKRNCDGYISRLQDLDQEIDTLYSRNDPGMILVTHQA
                     WDYFARDYHLDIVAIENDGKEATAKDLASFILLARSHGIRVVFAEAQESTREAETIAG
                     EIGGSVRVIDPLAGDYLANMERVAEAFGGAAQG"
     gene            1894..2661
                     /locus_tag="PK154_06950"
     CDS             1894..2661
                     /locus_tag="PK154_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10836.1"
                     /translation="MNGPVIELESVSCLLSGRTVLDAVDLKVREGDLYAVIGPNGGGK
                     TTLLKVILGLLPASSGTVRVFGTDPRKARSRIGYVPQYRTFDFRYPLTVEEMVLSGRL
                     GLVPGLVRRFSEKDRKAARRALERTAILHLSRRPICDLSGGEQQRAIIARALVGEPDL
                     LILDEPMVYVDMPTEMQLFDMLKALKDEMTVLLVTHDIGAISTNVTRVACLNGKIFTH
                     DTSEISEDMLTAAYHCPVDLIAHGIPHRVFRDHGGVG"
     gene            2658..3488
                     /locus_tag="PK154_06955"
     CDS             2658..3488
                     /locus_tag="PK154_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPW10837.1"
                     /translation="MILSILGFEFFRNAVAAGLIASIVCGIIGSFVIVKRLVSLSGGL
                     AHAAFGGVGLGYFLGVDPFLGAAGFTLAIALLIGTIREKFGQYMETLTGVVWAAGMAT
                     GILFIALTPGYAPELFGFLFGNILLIPSEDLIIMGILALGILLIVTAGFNQLVAVTFD
                     EEYARVMNLPVTALLLLLLLLIAFSVIVLLRVVGIILVIALLTIPPAIAREYSGSMRS
                     MMCLSVLAAAGCTIGGLFLSYAMNVPSGATIILLATVGYGLMLFERWMTGRRTQGAPG
                     "
     gene            complement(3617..5257)
                     /locus_tag="PK154_06960"
     CDS             complement(3617..5257)
                     /locus_tag="PK154_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPW10838.1"
                     /translation="MTGSEHPAGLSRERVVAFLDIGTNSIRLLIVRLFPHCTYHVLSR
                     QKETVRLGDGEFEEDLIRDEAIDRAVGVCRNFTSLARSFSADEVVAVATSAAREARNR
                     NVLLNRLRHEAGIDVRIISGRDEARLIYMGVATGIQLGKKQAVFIDIGGGSTEIAIGG
                     QHTSCHLESLLLGSIRLTNLFFSRDDEKPVTKKKYARIRDHVREVIAPALPGLSSHQF
                     DIAIGSSGTIINLSEIAMKTGEGISTPETGLSRSELARVAGLLCSLPLPARRNVPGIN
                     PERGDIIICGAAILETLMEALSIDRIFSTQRGLQDGLLADYLTRMEEIPLIDSVSPRE
                     RSVLQLARSCGINEHHARTVARLAQELFSSGKQCGLHAYSSPEEELLVYATFLHDVGS
                     FISYHNHHIHSYYVIRNTDIPGFLPRELLMMAFIARYHRKKPPKKRTLAVLPLEPGDR
                     ERIRVLSALLRMAESLDRSHAGLIRHARFLSTSPTGAVLEITAVGECQLELSGIENEA
                     DHFERVFGKTLITRMVTADLEELHEWTGNYPTLLTHPG"
     gene            complement(5299..>6252)
                     /locus_tag="PK154_06965"
     CDS             complement(5299..>6252)
                     /locus_tag="PK154_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW10839.1"
                     /translation="IADRAGELPLVRGGLLLTIASGVLLTLTSSTLLIVVFRVIEGIG
                     AGLFVPAALSLINSRPDHETSSGYFMAMLNIGLVAGLIASGWLVEMTGVGVSGIATFT
                     LIGAIPTLASMVMQPGGGQPRPAREPIRNTGRRLLRVIQEYFWLWISTIVLLGITGAL
                     TSLYPEFSDLSPALVGLVIAGMSTATAIAVIVVSHAHLPPIPTIKGASLAMAAAVLCV
                     FFSPVFFILVGAIAGVVMIAQLAYLAESETRQGVVMGLFNAASYGGMTFLPVLAGFIA
                     ELTTFVLAFAIIALSAVFVAFTIGRCRCRLAPGAAGDPPSS"
BASE COUNT         1280 a         1818 c         1903 g         1251 t
ORIGIN      
        1 ggcagcgttt gtcaggagga gtgcacggat cctggcactc ccgatcacgg agagcggctc
       61 acgggagatc gctgcacgga gccgcggcac tccccggata gcaaaccgcc ttctgcgaag
      121 ggtgggggat tacgccctgg tcaggggtga cggcaggatc gatgaggatc ttgccgatca
      181 ggcgctcacc atgctcggag tggacctgaa cgggctcgat gaaactgatc ggcggatcct
      241 cagggtaatc gtcgaggatt ttggaggagg gcccgtcggg atcaagacga tagccatctc
      301 gatcggggag gatgtgcgca cgatcgagga ggtctatgag ccgtacctca tccagtcagg
      361 atttatcaag cgtaccccgc agggaaggga agccaccccg gcggcacgga gccatatcca
      421 gccggcatgg tggtgaattt tttggtatct ccccaaaatt tgatgagata gcccgcacaa
      481 ctctagggga tgatcccttt caccggcctt gagctctccc ccggcaaagc cgcctatctg
      541 aagtacctct ccctccaggg cggcagcgtg aagatatccg gcatcgcggg ctatttcgag
      601 gtcgatccat ccaccgtcac ccggaccatc accgacctta cccgggaggg gtacgtcgag
      661 catgagccgt atggaagggt tatcctgacg gatttcggga gaaaatacgg ggcgtttctc
      721 gtgaggaggc accggatcct tggccttgtg ctctcccatt acggcttctc cgaggaggag
      781 gcctgcaccg aggcctccag gatggagtgc tatctctcaa gggaggcggt caataaaata
      841 tgccgggccc ttggtcatcc caccagggga ttctgcgggg agatctgcca tgatccgctc
      901 tgcctttcag gaacctttca tgggctgcat gaaaatgtga gctgatatgc aaaaaaatgg
      961 tgcttccatg ataccgcgga gcggggtgat ggccctggcc ctggttctcc tcgtcctggc
     1021 tgccggatgc aacggtgtcg ccaggaacga tcccggagaa cagatcagtg ttgcggtcac
     1081 catccctccc caggaggaga tggtgcggga gatagggggc gagcgagtcg aggtggtggt
     1141 gatggtgccc cccggatccg atccccatac ctacgagccg gagcctgcgc tggtggcacg
     1201 agctgccggg gccgatctgt acgtgacaat cgggacaggg ctgcttccca tcgaggatac
     1261 tcttgtatca cgattgacgg ctatgaaccc aggcctcgtt gttgctgaca gcagcacggg
     1321 tatccccccc ctctccccag accagggagg agaaaccagc ctggttcagg gggaagatct
     1381 cggaagagag acaggccagg cgaacccgag ccccgatcct cacatctggc tctcgctccg
     1441 caatgccgtg atcatgtcgg agaacaccag ggatgccctg atcaaggtcg atcctgccaa
     1501 tgaaaacgta tacaaaagga actgtgacgg atatatctcc cgcctgcagg atctcgatca
     1561 ggagatcgat actctgtact cccggaatga cccagggatg attctcgtca cccatcaggc
     1621 atgggactat tttgcgaggg attatcatct cgacattgtc gcaatcgaga atgatgggaa
     1681 agaagcgacg gcaaaagacc ttgcctcgtt cattcttctt gcccgatcac acgggatccg
     1741 ggtggtattt gccgaggccc aggagagcac ccgggaagcc gagaccattg ccggggagat
     1801 aggcggctcg gtcagggtca tcgatcccct ggccggggac taccttgcca acatggaacg
     1861 ggtggcagaa gcgtttggag gggcggccca gggatgaacg gcccggtgat cgaactcgaa
     1921 tcggtctcct gccttctctc aggacgaacg gtgcttgatg cggtcgacct gaaggtccgt
     1981 gaaggagacc tgtatgcagt gataggcccc aacggcggcg gaaaaaccac gttgctcaag
     2041 gtcatactcg gacttctccc tgccagctcc ggaacggtgc gggtctttgg gacggacccc
     2101 cggaaagccc ggagcaggat cgggtacgtc ccccagtaca gaacgttcga cttccgctac
     2161 ccgctcaccg ttgaggagat ggtgctttcg ggacggctcg gccttgttcc cggcctcgtc
     2221 aggcgattct ccgaaaaaga ccggaaagcc gcaaggaggg cgcttgaacg gaccgcgatc
     2281 cttcacctct cccgccggcc gatctgcgac ctctccgggg gtgagcagca gcgggcaatc
     2341 atagcccgcg cccttgtcgg cgaaccggac ctgctcatcc tcgatgagcc catggtgtat
     2401 gtcgatatgc ccaccgagat gcagttgttc gacatgctca aagccctcaa ggatgagatg
     2461 accgtccttc tggtcacgca tgacatcggg gccatctcca cgaatgtcac gagggtagcc
     2521 tgcctcaacg gtaagatctt cactcatgac accagcgaga tatcggagga tatgctcacc
     2581 gcggcatatc actgccccgt tgacctgatc gcccatggga tcccgcaccg ggttttccgt
     2641 gatcacgggg gggtgggatg atactttcga tcctggggtt tgaatttttc aggaacgctg
     2701 tggcagccgg gcttatagca agcatcgtct gtggcataat cggttcattt gtgatcgtca
     2761 aacgcctcgt atccctctcg ggggggcttg cccacgccgc gttcggcggt gtcggtctgg
     2821 ggtattttct cggggtcgat cctttcctcg gggctgccgg attcaccctc gcaattgccc
     2881 ttctcattgg gaccatccgc gagaagttcg ggcagtacat ggagacgctg accggcgtgg
     2941 tatgggctgc ggggatggcg accgggatcc tctttattgc actgaccccg ggctatgcac
     3001 cggaactgtt cgggttcctg tttggcaata ttctcctgat cccttcagaa gatctgatca
     3061 tcatgggtat cctggcactg ggtatcctcc tcatcgtgac cgccgggttc aaccagctcg
     3121 tggcagtcac gttcgatgag gagtacgccc gtgtgatgaa cctcccggtg acagccctcc
     3181 tcctcctcct cctcctgctc atcgccttca gcgtgattgt cctcctccgg gtcgtgggaa
     3241 ttatcctcgt cattgccctt ctcaccatcc cgcctgccat cgcccgcgag tattcgggga
     3301 gtatgcgatc catgatgtgc ctctcggtcc tggccgctgc gggatgcacc atagggggac
     3361 tcttcctgtc ctatgccatg aacgttccgt ccggtgcgac gatcattctc ctggcaaccg
     3421 tcggatacgg gctcatgctc ttcgagcgct ggatgaccgg gagaaggaca cagggtgcac
     3481 caggataagg tgcccctcct cattccgtcc ctgaaaaaaa ccgaacaacg ccaggggatt
     3541 tcccggaatg gcccctcccg catggtgcaa tgccgctgta ctggcttgcc cccccctccg
     3601 gtattcctga cttcgtctac cccgggtgcg tgagcagggt gggatagttc ccggtccatt
     3661 cgtgcagttc ttcaagatcg gcagtgacca ttctcgtgat gagtgtcttt ccaaacacgc
     3721 gttcgaaatg atcggcctcg ttctcgatcc ccgacagttc aagctggcac tcgccgacgg
     3781 cagtgatctc gagcacagca cctgtcggac tggtggagag gaaccttgca tgccggatca
     3841 gcccggcatg gctccggtca agactctccg ccatccggag cagggctgag aggaccctga
     3901 tccgctcccg atctcctggc tcgaggggaa gtaccgccaa agtcctcttt tttggcggct
     3961 tcttccggtg atagcgggcg atgaacgcca tcatgaggag ttcccggggg agaaaaccag
     4021 ggatatcggt gttcctgatc acgtaatagg agtggatgtg gtgattgtgg taggagatga
     4081 aggatcccac gtcatggagg aacgtggcat agacaaggag ttcctcctcg gggctggaat
     4141 atgcatgcaa cccgcactgc tttccgctgg agaacagctc ctgggcgagg cgtgcaacgg
     4201 ttcgtgcatg atgctcattg ataccgcagg agcgggcgag ctggaggacg cttcgttccc
     4261 gcggggatac gctgtcgatc agggggatct cctccatccg tgtgaggtaa tctgcaagaa
     4321 gaccatcctg caaccctctc tgggtggaga agatccggtc gatggagagg gcctccatca
     4381 gggtttcaag gatggcggcg ccgcagatga tgatatcccc ccgttccggg ttgatacccg
     4441 ggacgttcct cctggcaggg aggggaaggg agcagaggag accggctacc cgtgccagct
     4501 ctgaacggga aagcccggtc tcaggggtgg atattccctc cccggttttc atggcaatct
     4561 ctgaaaggtt gatgatggtg cccgagctgc cgatggcaat gtcgaactgg tgggaggaga
     4621 gccctggtaa agccggggcg atcacctccc tcacatgatc ccggatgcgt gcgtacttct
     4681 tttttgtgac cggcttctcg tcatcgcggg agaagaagag gttggtgagc cggatcgatc
     4741 ccaagagcag gctttcgagg tggcaggagg tatgctgccc tcctatggcg atctctgtgg
     4801 aacccccccc gatgtcgatg aagaccgcct gtttcttccc gagctggatc ccggttgcca
     4861 cccccatgta gatgagccgg gcttcgtccc ttcccgagat gatccggaca tcgatcccag
     4921 cttcatgacg caggcggttc aggagcacgt tccggttccg cgcctcccgt gccgcggacg
     4981 tggcaaccgc taccacttca tcggctgaga aggatcgggc aagggaggtg aaattccggc
     5041 agaccccgac agcccgatcg atagcctcgt cacgaatgag atcctcctcg aactctccat
     5101 cgccaagccg gaccgtctcc ttctggcggg aaagaacatg gtaggtgcag tgggggaaga
     5161 gacggacgat cagcagccgg atcgaattgg taccgatatc gagaaaggca actacgcgtt
     5221 cccgtgatag cccggcggga tgttctgaac ctgtcattcc tgcctaaact tctctttcta
     5281 gcaccatgaa atgcggggtc aggaggatgg aggatccccg gcagctcccg gagcgaggcg
     5341 gcaccggcac ctcccgatgg taaaggctac gaataccgca gaaagagcga tgatggcgaa
     5401 tgccaggaca aacgtggtca gttccgcgat gaatcctgca aggaccggaa ggaacgtcat
     5461 tcccccgtaa ctggccgcat tgaacagtcc catcaccacc ccctgccggg tctcagactc
     5521 ggcaagatac gccagctggg cgatcatcac cacccctgca atcgcgccga ccaggatgaa
     5581 aaagacaggc gagaagaaga cgcagagcac ggctgccgcc atcgcaagag atgctccttt
     5641 tattgtcgga atcgggggaa ggtgggcatg ggataccacg atcacggcga ttgcagtggc
     5701 ggtgctcatc ccggctatga ccagtccgac aagggcgggt gagagatccg agaattccgg
     5761 atagagggag gtgagcgccc ctgttatccc gaggagcaca atggtcgaga tccagagcca
     5821 gaagtattcc tgtatcacac gaagcagcct tctccctgta ttccggatcg gttcacgggc
     5881 cgggcgtggc tgtccccctc ctggctgcat gaccatgctc gcgagggtgg ggatcgcccc
     5941 gatcagggtg aaggtggcga tgcccgacac gccaacaccg gtcatctcaa caagccagcc
     6001 gctggcgatc agccctgcga caagaccaat attcagcatc gccatgaaat atccgctcga
     6061 ggtctcatgg tcaggccgtg aattgatcag ggagagggca gccgggacga acagcccagc
     6121 cccgatcccc tcgataacac ggaacaccac gatcagaaga gtggagctgg taagcgtgag
     6181 gagcacccct gaagcaatgg tcagcaggag acctcccctc acgagaggca gttcgcctgc
     6241 acggtcagca at
//
LOCUS       DAOOCO010000110         6232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11806_length_6232_cov_5.517889, whole genome shotgun sequence.
ACCESSION   DAOOCO010000110 DAOOCO010000000
VERSION     DAOOCO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11806_length_6232_cov_5.517889"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PK154_06970"
     CDS             <1..344
                     /locus_tag="PK154_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW10840.1"
                     /translation="RAELASCEEEVRELYREAQSGLTGATPSSLAIRRQVSQLTYSRS
                     CPEASAIAACRAAGIRVEPGMEIGYVVTDARTWSVELDWLAKRFDQGYYRRLLARAWE
                     EIAFALEQAAK"
     gene            complement(448..1353)
                     /locus_tag="PK154_06975"
     CDS             complement(448..1353)
                     /locus_tag="PK154_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPW10841.1"
                     /translation="MNTSRSAFLLLVLAACCLIAGCSTDTGFPGGSPLPAGQSETATV
                     TRTIDGDTVYLSFPDGRAETLRILGIDTPEMTPEGNDPGKFRGITDPEFLSIWGEEAA
                     SYTNEKLTGKIVTISYDPAAGRRDTYGRLLATVILPDGTNHGEDLIRQGLARVFTPET
                     FALKDHYLSAQEEAMDARRGVWSGGEPDGTGTGQVVIASVHYNAAGDDAANLNDEYLT
                     IRNRGTTVADLTSWQISDADSFSYPMPPVALPPGGAIRIHAGNGTPSAGELYMDLDGP
                     VLNNDGDTVTLLDREGTVVSTYSWA"
     gene            1488..2705
                     /locus_tag="PK154_06980"
     CDS             1488..2705
                     /locus_tag="PK154_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPW10842.1"
                     /translation="MADEDETGKLYHEALELHARYHGKIELHPKVPVETRRDLARAYT
                     PGVAEVCREIAKDRDLAYRYTMKANTVAIVTDGSAVLGLGNIGGYAAIPVMEGKALLF
                     KKFAGIDAFPICFESYHTDFIDEVKNIAPVFGGINLEDIAAPKCFEVEEALQEIGIPV
                     MHDDQHGTAITVLAALLNSCRVLGKEFEDLNIVVSGAGAAGYAIVRLLRCIGYSPDVC
                     QRVRDIIVCDTQGIIHRNRKGLYANKYKFIIADETNHAGRTGGLTEALEGADVFIGVS
                     APGLLKAEMIRSMNDDPIVFAMANPVPEIMPEQARAAGAAIIGTGRSDYPNQINNALA
                     FPGVFRGALDVHARKIDDDMKVAAAHALAGYVKRPARDHIMPPVLDRSVVKVVAAAVS
                     SAARGSGMARDVG"
     gene            complement(3028..3567)
                     /locus_tag="PK154_06985"
     CDS             complement(3028..3567)
                     /locus_tag="PK154_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW10843.1"
                     /translation="MTHPEVYAPAEDTWLLLRAAGKECHDGERVLEIGCGSGIISASL
                     SGRASVLATDINPHAVRAARDRGIEVVRTDLAAGICSRFDLILFNPPYLPTMPEERLD
                     DWLEYALDGGPDGRRVISRFLSSAGRLLAPGGRVLLLLSSLTGISEVCMLIREAGFSP
                     EWIMEETVEGELLTVIRFR"
     gene            complement(3564..4334)
                     /gene="rsmA"
                     /locus_tag="PK154_06990"
     CDS             complement(3564..4334)
                     /gene="rsmA"
                     /locus_tag="PK154_06990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW10844.1"
                     /translation="MRAPKDQHFLIDRNAVRRIADLVDLRDRLVLEIGPGEGILTGEL
                     LSRGAKVNAVEIDPDLVDELTLFFADEIETGRLTVICGNAIRCDLPPFDVVVSNLPYS
                     ASSKITFRLLAAGFEEAVLMYQEEFARRMVAPAGTPDCGRLSIMVQTYCCVERCFDLP
                     PQAFSPKPQVRSTVVRMKLRGPLFPFSDDALYADVVRALFSHRRKTVRNCLRNSAGIL
                     DPALAEKMLAALPDEILASRPETLYLEDFATITNLAAE"
     gene            complement(4331..4891)
                     /locus_tag="PK154_06995"
     CDS             complement(4331..4891)
                     /locus_tag="PK154_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HPW10845.1"
                     /translation="MKADKKEINAIVLDVLQRGHPDDPRPVFKREAIVQAMGISQFKL
                     LELVPKSLGIQIHERVYIGEGEREKIERVKRRIGYGELTQTARVELPFVVEQLVKEKE
                     PEFVQFFNKSISITPKLHMLHLLPGIGKKLMWEILTEREKKPFESFADISQRIKSIPH
                     PEKMVVNRILEELQDPDIKYHVFTSK"
     gene            complement(4902..5252)
                     /locus_tag="PK154_07000"
     CDS             complement(4902..5252)
                     /locus_tag="PK154_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HPW10846.1"
                     /translation="MKVKAVLSEERVTLPEVREVLAQVEAERIASEKEMSYELRKSIE
                     HANQTSKMPADKSRELVKELLTLEKMKPDIAYRIANTAPRSRDELRAIYAKEKFSLTG
                     EELDAIVGIVQNYL"
     gene            complement(5276..5566)
                     /locus_tag="PK154_07005"
     CDS             complement(5276..5566)
                     /locus_tag="PK154_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HPW10847.1"
                     /translation="MAHHNGPRKKTRYKFKKEIRRRGILPVTSLIQRFEPGQKVHVVC
                     EPSIQKGMPHRRFHGKTGTVVGQRGRAWLLAIRDGDKDKIVIARPQHLKAQR"
     gene            complement(5573..>6232)
                     /locus_tag="PK154_07010"
     CDS             complement(5573..>6232)
                     /locus_tag="PK154_07010"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HPW10848.1"
                     /translation="AVDAGAGAELLQDSVEELIGRPAIELCAAEAAVLHGAGREDIDA
                     RMIGTGRPFVMELVRPFRRSIDLKVLEEAINGSASGRVEVSLERWTRRGEVETIKSDK
                     AHKKYRILVEIEGPLSLYELQSALKALKGVTIYQRTPQRVAHRRADKVRERHATEIAY
                     LGEEEDGYVLEVTGEAGLYIKELISGDNGRTRPSLTEILQRPARVSRLDVVQVERTRE
                     E"
BASE COUNT         1269 a         1826 c         1823 g         1314 t
ORIGIN      
        1 cacgggctga gctggcatca tgtgaagagg aggtcaggga actctaccgg gaggcacaat
       61 ccgggctcac cggggccacc ccctcctcac tcgccatccg ccggcaggta agccagctca
      121 cctactcccg gagctgcccc gaggcatcgg ccatcgcagc atgccgcgct gcagggatca
      181 gggtggagcc ggggatggag atcgggtacg tcgtgactga cgccaggacc tggtccgtgg
      241 aactggactg gcttgcaaag cgttttgacc agggatacta ccggaggctg cttgccagag
      301 cctgggaaga gatcgcgttt gccctggagc aggcagcgaa gtaaaacaaa agggggcttt
      361 tgagggatcc tctgcatcgt ccccaggaga tactggcaga aggataccgg catgatgggt
      421 atccccgctc ttctcaggtt agatcgttca ggcccatgag taggtagaga cgaccgttcc
      481 ttccctgtcg agcagggtca ccgtatcccc gtcattgttg agcaccgggc catcgagatc
      541 catgtagagc tctcctgccg atggcgtccc gttcccggca tggatcctga tagccccgcc
      601 aggggggaga gcaacagggg gcatggggta ggaaaagctg tcagcatcgc tgatctgcca
      661 ggaggtgagg tcggcaacgg tcgtgccccg gttgcggatc gtgagatact catcgttcag
      721 gttagccgca tcatctccgg cagcattgta gtgcaccgat gcgatcacga cctgaccggt
      781 tcccgtcccg tcaggttccc cgccgctcca gacgcctcgc cgggcatcca tggcctcttc
      841 ctgagccgag aggtagtgat ccttcagggc gaacgtctca ggggtgaaca cccgtgcaag
      901 tccctgcctg atcagatctt caccatggtt ggtgccatcc gggaggatga ccgtggcaag
      961 gagccggccg taggtatccc ggcgtcccgc tgctggatcg tatgagatcg tgacgatctt
     1021 cccggtaagc ttttcattgg tataggatgc agcctcctct ccccagatcg agaggaactc
     1081 agggtcggtg atccccctga actttccggg atcgttcccc tcgggggtca tttcaggggt
     1141 atcgatccca aggatccgga gggtctctgc tctcccgtcc ggaaaagaga ggtacaccgt
     1201 atctccgtca atggtccgcg ttactgtcgc agtctcggac tggcctgcag ggaggggtga
     1261 tccccctggg aatccggtat ccgtgctgca tcccgcgata aggcagcatg cggcaaggac
     1321 aaggaggaga aaggctgatc tgctcgtatt catgagaatc cggcattaca ctggggcgct
     1381 ctcccagaaa aaacctcgtt atctgccggg agtgggacag gcaggatttt tatgctcatg
     1441 gctctgatgc actcatacga tgcacgggaa tgcggctggt gaacagaatg gcagatgagg
     1501 acgagacggg caaactctac catgaggcgc tcgaactcca tgcccggtac catggaaaga
     1561 tcgaactcca tcccaaggta ccggtcgaga caagaaggga ccttgcacgg gcttataccc
     1621 ccggtgtcgc agaggtctgc cgggagatcg caaaagaccg ggatcttgcg taccgctaca
     1681 cgatgaaagc caacacggtg gcgatcgtga ccgacggctc tgcagtcctt gggttgggca
     1741 acatcggggg gtatgccgcg atcccggtca tggagggaaa agcgctcctc ttcaagaaat
     1801 ttgccgggat cgatgcattt cctatctgtt ttgagagtta tcacaccgat ttcatcgacg
     1861 aggtcaagaa cattgctccg gtgttcggag gtatcaatct cgaggatata gccgcaccga
     1921 aatgtttcga ggtggaggaa gccctccagg agatcggcat cccggtcatg cacgatgacc
     1981 agcatgggac cgccatcacc gtgcttgcgg cgctcctcaa ctcctgccgg gtgctcggca
     2041 aggagttcga agacctgaac atcgtggtca gtggcgctgg cgctgccggc tacgccattg
     2101 tccgcctcct ccggtgcatc gggtacagcc cggacgtctg ccagcgggtc agggatatca
     2161 tcgtctgcga tacccagggg atcatccacc ggaaccggaa ggggctctat gccaacaagt
     2221 acaagttcat cattgccgac gagaccaacc atgcagggcg gacaggcggg cttacagagg
     2281 ctctggaagg tgcggatgtt ttcattgggg tgtccgcccc gggcctcctg aaagccgaaa
     2341 tgatccggtc gatgaacgat gatccgatcg tctttgccat ggcaaacccg gtccctgaga
     2401 tcatgcctga acaggcaagg gcagcgggtg ccgctatcat cgggaccggg agatccgatt
     2461 atcccaacca gatcaacaat gccctcgcgt tccccggcgt gttccgtggg gcacttgacg
     2521 tgcatgccag aaagatcgac gacgacatga aggttgcagc ggcccatgca ctcgcggggt
     2581 atgtgaagag accggcacgg gaccatatca tgcccccggt cctcgacagg agcgtggtga
     2641 aggtggtggc cgctgcggta agcagcgctg cccgtggatc agggatggca cgggacgtcg
     2701 gataaacgcg gcatctctct ctttttcatg cggggctcca ggatggcaat gtcggtcttc
     2761 tctttcagga ccgcaggagg gaagaagtca tgatcagaag aaagccgttg accgggcgac
     2821 catctccctc gcaaaggaga accaggcgtt cgtagggagc atactcatct tccttatcca
     2881 tgagcaaggc gggagaattt accggttacc gggacgattc gctctgtaaa gaagtgggga
     2941 acatcctctg aacagaatcg gggaactggt cccgggggag taacaatgcc tgatagtccc
     3001 ctggctggcg ggaaagatcc ccgcagttca gcggaacctg atcacggtca gcagctctcc
     3061 ctctactgtc tcctccatga tccattccgg ggagaagcca gcctcccgta tcagcatgca
     3121 gacctcggaa atcccggtca gggacgagag aaggagaagc acccgcccgc ccggggcaag
     3181 cagcctccca gcactggaca ggaaccggga gatgaccctg cgaccatccg ggccaccatc
     3241 gagggcatat tcgagccagt cgtccagccg ctcttccggc atggtcggga ggtagggtgg
     3301 gttgaagagg atcaggtcaa accggctgca gataccggca gcgagatcgg ttctcaccac
     3361 ctcgatccca cgatcgcggg ccgcacggac cgcgtgggga ttgatgtcgg tagcgagtac
     3421 gctggcccgc ccggaaagag acgctgaaat aataccgctg ccacatccga tttcaagcac
     3481 ccgttcacca tcatggcatt ccttcccagc ggcccggagg aggagccagg tgtcctcggc
     3541 cggggcatag acctccgggt gggtcattct gccgccaggt tcgtgatggt cgcaaaatcc
     3601 tcgagataga gcgtctccgg gcgggaggcg aggatctcat cgggcagggc ggcaagcatc
     3661 ttctctgcaa gagcaggatc gaggatcccg gcagaattcc gcaagcagtt ccttaccgtc
     3721 tttctccggt gggagaagag ggcccggacc acatcggcat agagtgcatc gtcactgaag
     3781 gggaagaggg ggccgcggag cttcatcctc accactgtcg atctgacctg aggcttcgga
     3841 gagaatgcct gcggggggag atcgaagcac cgctcgacac agcagtaggt ctggaccatg
     3901 atcgagagcc gcccgcaatc aggggtcccg gccggtgcaa ccatccggcg ggcaaactcc
     3961 tcctggtaca tcagcaccgc ttcttcaaat ccggctgcaa gcagccggaa cgtaatcttt
     4021 gacgatgcgg aatagggaag gttcgagacc accacatcaa atggcgggag gtcgcaccgg
     4081 atcgcgtttc cgcagatgac cgtaagcctt cccgtctcga tctcatcagc aaaaaagagg
     4141 gttagttcgt cgaccagatc gggatcgatc tccacggcat tgacctttgc cccccgggag
     4201 aggagctcgc ctgtcaggat cccttctccg ggaccgatct cgaggaccag acgatctcgg
     4261 agatcaacca gatcagctat ccgacggact gcattcctgt cgatcaggaa gtgctgatcc
     4321 tttggggctc tcattttgag gtgaagacat gatacttgat atccgggtcc tggagctcct
     4381 cgaggatccg gttcaccacc atcttttccg gatggggaat cgacttgatc cgctggctga
     4441 tgtcagcaaa gctctcgaac ggcttctttt cccgctcggt gagaatctcc cacatcagtt
     4501 tctttccgat ccccggcagg aggtggagca tatgcagttt cggggtgatc gatatggact
     4561 tgttgaagaa ctggacaaat tcaggttcct tctctttcac cagctgctca accacaaagg
     4621 ggagttcgac cctggctgtc tgggtcagct ccccgtagcc gatccgccgt ttcaccctct
     4681 ctatcttttc tcgctcaccc tccccgatat agacccgctc gtggatctgg atacccagtg
     4741 atttcggcac cagttcaagg agtttgaact gggagatccc catagcctgg acgatcgctt
     4801 cacgtttgaa gaccggccgg ggatcatcgg gatgcccccg ctggagaaca tcgaggacta
     4861 tcgcgttgat ctctttttta tccgccttca tgaaaaagac ctcagagata gttctgcacg
     4921 ataccgacaa tggcatcgag ctcttcgccg gtgagagaga atttttcctt cgcatagatc
     4981 gcccggagtt catccctgct ccggggtgca gtattggcaa tacggtatgc gatatcgggt
     5041 ttcatcttct cgagcgtgag aagttctttt accagttccc gggacttgtc ggccggcatc
     5101 ttggaggtct ggttggcatg ctcgatgctt ttgcgaagct catatgacat ctctttctct
     5161 gatgcgatcc gctctgcctc gacctgggcg aggacctcac ggacctccgg gagagtgacc
     5221 ctctcttcgc taagaacagc ctttaccttc atgccagtca cctgattcca gagatttacc
     5281 tttgtgcttt tagatgttgc ggtcttgcga tgacgatctt gtccttgtcg ccatcccgga
     5341 tggcaaggag ccaggcgcgc ccgcgctggc cgaccaccgt tccggtcttt ccatggaacc
     5401 tgcggtgcgg catacctttc tggatgctcg gctcgcagac cacgtgcacc ttctgaccag
     5461 gctcaaatcg ctggatgagc gaggttaccg gcaggatgcc acgccgtcgt atctctttct
     5521 tgaatttgta ccgtgtcttc ttccttggtc cgttatgatg tgccatgatt actcactcct
     5581 ccctggtgcg ttctacctgc accacatcga gcctcgagac ccttgcaggc ctttggagga
     5641 tctcggtaag acttggccgg gtccgtccgt tatcacccga gataagttcc ttgatataga
     5701 gaccggcttc tccagtcacc tcaagaacat acccgtcctc ttcctccccg aggtatgcta
     5761 tctccgtagc gtgccgttca cgtactttat cagccctgcg gtgagcgacc ctctgtgggg
     5821 tgcgctgata tattgtcacc cctttcaggg cttttagggc tgattggagc tcatataagg
     5881 acaacgggcc ttctatctcg accaggatcc tgtatttttt atgcgctttg tccgatttaa
     5941 tggtttccac ctcaccgcgc cgtgtccatc gctcaaggga tacttctacc ctgcctgaag
     6001 cggacccgtt gatcgcttcc tcgagcacct ttagatcgat actccggcgg aacggccgga
     6061 ccagctccat cacaaaaggc cggccggtgc cgatcatccg ggcatcgata tcctctcgcc
     6121 cggcaccatg gagcaccgcg gcttccgcgg cacagagctc gatcgcgggc cgcccgatca
     6181 gctcctcgac cgaatcctgc aacagctcgg cgccggcgcc tgcgtccacg gc
//
LOCUS       DAOOCO010000111         6201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11885_length_6201_cov_5.516759, whole genome shotgun sequence.
ACCESSION   DAOOCO010000111 DAOOCO010000000
VERSION     DAOOCO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11885_length_6201_cov_5.516759"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK154_07015"
     CDS             <1..396
                     /locus_tag="PK154_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10849.1"
                     /translation="IFVNLPVKDLDRSVLFFSRLGFTFNAQFTDQNATAMVISEDIYA
                     MLLVESFFKTFTKKAICDTKKSTEVIVALSADSRELVDAMVDKAIAAGALSPEGSKDY
                     GFMYQRGFQDLDGHLWDIFYMDPDYVEKP"
     gene            complement(1004..1969)
                     /locus_tag="PK154_07020"
     CDS             complement(1004..1969)
                     /locus_tag="PK154_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPW10850.1"
                     /translation="MDGGYFSEWLVSYRHYLRMRNYSPRTIQSYEQVVRHFASYVWLR
                     RNNPEKIELFWEDFSNARLDTTVEVKPVLITDYFTFINTKKPYKAKTTHRIISTLSSF
                     YRFLYSQGVITANPMVAIERPKIKDQELRYLKHSQVLRLIASIEDDRDRLIVRTIYAT
                     GVRVSELCGINIEDIDFEDEMIKVRGKGGKIRIVFVDHETLEEISRFIGNRIEGPLFL
                     GQMGHHISPRSVQHIFKKYAPQGITPHKIRHSYASELYRRSKNLRVVQENLGHTSIKT
                     TEIYLHTDLDERRKVYQEFFPLSENSAPPPPKPEKKEDQPEPDPL"
     gene            3710..4243
                     /locus_tag="PK154_07025"
     CDS             3710..4243
                     /locus_tag="PK154_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HPW10851.1"
                     /translation="MAQQFGQDDVNKYRKFRKVDGATYRKVNQFLRKRTYITAREWAI
                     ARLCTDFRTRNGAEMTFIGLHLPELVPFMQETYSPQAVNQARNSFKKKVTKSGATFFY
                     GAMCGFFTSEELDDLLFEASEVARFLLEVERSSLDIDEEIDIEDRISGIMRSVGESAA
                     ALIRTRAEDEEKRAGES"
     gene            4243..4950
                     /gene="mobB"
                     /locus_tag="PK154_07030"
     CDS             4243..4950
                     /gene="mobB"
                     /locus_tag="PK154_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPW10852.1"
                     /translation="MKIIHIAGFSNTGKTTFIRELVPELEKRGLTGVVKHIGHHGHSL
                     PEGKDTTLFHEAGAAASAGIDAQKSVLILKETDLERILAMFCDAGVQYAVIEGFKEKP
                     YPKVVIGNIANATNVLLTNPSVEEVLSHLGEFSDMVTREGFFREIEAGCDPGRAIQVS
                     ATPMTIPGDQITSLERESGTLVRDLHEVSIRFASTTTPGARTLLTGICAPDARTAIEA
                     ATRINSLMLSPLPGYKE"
     gene            4955..>6201
                     /gene="glmM"
                     /locus_tag="PK154_07035"
     CDS             4955..>6201
                     /gene="glmM"
                     /locus_tag="PK154_07035"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPW10853.1"
                     /translation="MTGKRLFGTNGVRGITGADMTPTLALSIGLALGSMRTGSIAVGR
                     DTRTSGPALCSAVKAGLLATGCDVTDCGILPTPALQYLVKEHFDAGAMITASHNPPEY
                     NGVKVIEPDGTEMGDEETIRLEAKILDERVPLSPWNATGREVHASHLVREYTDAVVKK
                     FPEGIGEGITVAVDPGCGAACATTPEILTRLGCRVVTLNSQMDGTFPGRLPEPSPEGL
                     RPLGNLVRSTGAALGIAHDGDADRAVFLDERGEFIEENQEFGIIAGQVCRDRKGTVVM
                     PVSTSRLVEEIARRNGCDVQYTPVGSIYVARAMRALEAAHRPVAFGGEGNGGLIYPDH
                     QYCRDGGMTAAMMVAILAREHGPASDLVAALPSFTLIKEKIRGADPSAMIRNLCTAFA
                     GDHIDRTDGIRINRDESWALIRPS"
BASE COUNT         1449 a         1697 c         1708 g         1347 t
ORIGIN      
        1 atttttgtca atttaccggt gaaggatctc gaccgatcgg tcctattctt ttctcgcctt
       61 ggttttacgt tcaatgccca gtttaccgac cagaacgcca cggccatggt gatcagcgag
      121 gatatctacg cgatgctgct tgtggaatcg ttcttcaaga catttaccaa gaaggcaatc
      181 tgtgatacga agaagagtac ggaagtgatt gttgctttat ctgccgatag cagggagctt
      241 gttgatgcga tggtggacaa ggccatcgca gcgggggcct tgtcaccgga aggatcgaag
      301 gactacggtt tcatgtacca gcggggtttt caggacctgg atggccacct ctgggatatc
      361 ttctatatgg acccggatta tgtagagaaa ccctgacctt tttataagaa aatgcgtccg
      421 gcattttcat cccggcctgc tggtattcct caatggaatc aattgatttt tccgggatga
      481 gacagtgaag aaaaaaatcg cctaaaaagg cttaattttg atgaaatcta tgtattgcct
      541 gttattcggt tgggggttgc actatcccgc catgaacttg ccggatcaat cagttccgat
      601 aataatcact cattttgaag ttattttttc aaataatcat gattcgtccg ttttctcccc
      661 cactcctttt cattcagata gctctatctc agaaaatcga tacgaacgca ttggaacgcg
      721 atcgggatat gcaaaatgct gcactgagga aaaaaagatt catctttgtg tatagcatta
      781 tacggtttat ttttcagaag agcacgacgg aagagaaaag gtaagcgggc gatgtgcggg
      841 aatatttttc aaattcgtgg agaatggtga gagtaaactc cctgcaggaa tacgggaaca
      901 atgggggttt gttctgaggg tgctcctggt cctgcaggct gaatatcatg attgtacagc
      961 ttaggtacct gagagaaagg cagtatcgca cattcacccc attctatagg gggtctggtt
     1021 ccggctgatc ctccttcttt tccggcttgg gaggcggagg ggcactgttc tctgagagtg
     1081 ggaagaactc ctggtagacc ttcctccgct cgtcgaggtc ggtgtggagg tagatctcag
     1141 tcgttttgat cgaggtatgc ccgaggttct cctggaccac ccggaggttc tttgaccgtc
     1201 ggtagagctc gcttgcatag ctgtgccgga tcttgtgagg ggtgatcccc tgcggggcat
     1261 acttcttgaa gatgtgctgg accgaccggg gggagatgtg gtgccccatc tgccccaaaa
     1321 agagcggtcc ctcgatccgg ttgccgatga accgggagat ctcctcgagc gtctcgtggt
     1381 cgacgaagac gatccggatc ttgcccccct tcccccggac cttgatcatc tcatcctcaa
     1441 aatcgatatc ctcaatgttg atcccgcaga gctctgagac ccggaccccg gtggcataga
     1501 tcgtccggac gatcagccgg tcccggtcat cctcgatgga ggcgatcagc cggagcacct
     1561 ggctgtgctt caggtaccgg agctcctggt ccttgatctt cggccgctca attgcgacca
     1621 tcgggtttgc cgtgatcacc ccttgggaat aaaggaaccg gtagaacgag ctgagcgtcg
     1681 agatgatccg gtgggtggtc ttcgccttgt acggcttctt ggtattgatg aacgtgaaat
     1741 agtccgtgat gaggaccggc ttcacctcaa ctgtcgtgtc gagcctggca tttgagaagt
     1801 cctcccagaa gagctcgatc ttctcaggat tgttcctccg cagccagaca taggaggcaa
     1861 aatggcggac cacctgctca tagctctgga tggtccgggg ggaataattg cgcatccgga
     1921 ggtagtgacg gtaactcacc agccattcgg aaaaataacc cccatccatg ctctattctt
     1981 tataacgact gataattaaa gttttgcgca gaatacacat ttgccgcaat gaatccccga
     2041 ttccaggaaa aatcagcagg ctttccagga tccctgattc ctggctcatc atacctgaga
     2101 cagttctccc cagcacgaat tatcagggca tgatcctccc ctgcatgctc agggaaccac
     2161 catcatgatg cgggacctga tacgataccc attatccccc atccatgaag aagtaatacc
     2221 tattcgcaga accgggctca tcctcatcgt cgaagaatct cagaaagccc catagattcg
     2281 ttctgttggc agatcgagac cccccgcaat aggctggagg gtcaaaatga aaagaatgcc
     2341 tgtttaggag agggggagaa ggcatcccag gagacctcca ggacgtgaac gggaacggtc
     2401 ccggagtact cttcatgttc ctgcttatca gtcagaaagt atcgcagagc gggtcaagtt
     2461 gatggagacg caggataacc aggtttaact tgttttcaga acgaagggat gcctcgggcc
     2521 ccgcaagaat cagtggcatg ggcaagggca tacccctgag cattcgtcgg gttggagagg
     2581 atcacctacc ttaacaccat ggagcaggtt tgcgtggtcg actcccgaaa aagttcagca
     2641 cgcggcaggg taccctactg gtgctgcagg tttgcccgaa ccgatcttct gcattaacac
     2701 ctcccggcca tcagtctgtt caaccggtga ggatcgagag gaagggtttg aaccctggca
     2761 tgaaaccttc aggctcatcc gtggttcaac gatctcttcc tttccccgca ttgataccga
     2821 ccggtgataa ggccggagaa tgacgaattg cgacctgcta tgataaacag ggaattgttc
     2881 gatgcgatga cgagtgttgc ggtactccct cgacaagatc gattcccgag cgtgctcctc
     2941 cacacgcctc aacaccgcta ccgggtgaat cactcctcag agggatacca tggagccggc
     3001 ctgaagccgg agtcacggat gagaagaggt cctgccatcc cgggaggcac cctaaaaagg
     3061 atcacaccga taccaccttc gcaatcgata tgagaacagg gtgcccaccc attgtgtccc
     3121 ctcgtccacc agcagtcttt ttacgctcat ttgccgcgtg ggctgatgtg cactcgtatc
     3181 gtatcccctc acccatcaac aaagtcagga atcataccag gagaaaaaat ccgcatgctg
     3241 atgcatgatt ccgtgttctc tcatacaggc gggcaccctt atcgtttcat cttcagcgtt
     3301 tttacggcaa tcaagaacca ataagaccgg aggtcaacgg gaaacgaatc acagggattc
     3361 gggatcatgg tacacccgga ggggacgacc cgtccgttct ggagggtaat aatgccccgc
     3421 ccgctccggg actcgtcccg attcgggaaa gagagcagcg gcaatgaggg tgctgcttac
     3481 ctatcggcac cctgttggat ggtgaatcac caggattctt caggtgggtg aaaaagattc
     3541 tattcagccc catattctga aaataattca aaatattcgt ttgattcact caccaggccg
     3601 gttgcgcgtt ccacaaaata aaccatatga tgctatatac taaagaggtg cctgagcact
     3661 ccaatcccgc atcctataat aaatcctgtg cgcctaacgt actgatcaga tggcgcagca
     3721 gttcggtcag gacgatgtca acaagtacag gaaattcagg aaggtggatg gcgccaccta
     3781 ccggaaagta aaccagttcc tgcggaagcg gacctatatc actgctcgcg agtgggcgat
     3841 cgcccgcctc tgcaccgatt tccggacacg gaacggggca gagatgacct tcatcggact
     3901 gcacctgcct gaactcgtcc cgttcatgca ggagacctat tcgccccagg cggtcaacca
     3961 ggcccggaac tccttcaaga agaaagtcac caagtccggg gccaccttct tttacggcgc
     4021 catgtgcggg ttcttcacct cagaagagct cgatgacctc ctctttgagg caagtgaagt
     4081 tgcacggttc ctccttgagg tagagcgctc atcactggat atcgacgaag agatcgatat
     4141 cgaggaccgg atctcgggga tcatgagaag tgtcggagag tccgctgctg ccctgatccg
     4201 cacccgggca gaagacgagg agaagagggc aggcgaatcc tgatgaaaat aattcacatt
     4261 gccggctttt ccaataccgg gaagaccacg tttatcaggg aactggtgcc ggaacttgaa
     4321 aaacgtgggc tgaccggcgt ggtaaaacac atcggccacc acgggcactc ccttcccgag
     4381 gggaaagata ccaccctctt ccacgaggcg ggagctgcgg catcggctgg tatcgatgcg
     4441 cagaaatccg tcctgatcct gaaggagact gacctcgaac gaatccttgc gatgttctgc
     4501 gatgcagggg tgcagtacgc cgttatcgaa ggattcaagg aaaaacccta cccgaaagtc
     4561 gtgatcggga acatcgccaa cgcaacgaat gtccttctca ccaatccgtc cgtagaggaa
     4621 gtgctctcac atctcgggga attctcggac atggtcacgc gggagggttt tttcagggag
     4681 atcgaagccg gctgtgatcc cggcagggcg atacaggtct cggcaacccc gatgaccatc
     4741 ccgggagacc agatcaccag ccttgagagg gagtcaggga ccctggtcag agatctgcat
     4801 gaagtgtcca tccggttcgc gtcgacaacg acccccggag cccggacgct cctgaccggg
     4861 atctgcgccc ctgatgcacg gaccgcaatt gaagcagcaa cgaggatcaa cagcctcatg
     4921 ctctctcccc tgccaggata taaggagtga tccgatgaca ggaaaacgac tatttggtac
     4981 aaacggcgtc agggggataa caggggctga catgacccct acccttgccc tctccatcgg
     5041 tcttgcgctc ggatcgatgc ggaccggatc catcgcggtc ggacgggata cccggacctc
     5101 cgggcctgcc ctctgcagtg cagtcaaggc agggctcctt gccacagggt gtgatgtgac
     5161 cgattgcggg atacttccca cgccggcatt gcagtatctg gtgaaggagc atttcgatgc
     5221 cggcgccatg atcaccgcat cgcataaccc ccccgaatat aacggggtga aggtgatcga
     5281 gccggacgga accgagatgg gagatgagga gaccatcagg ctcgaggcaa agatccttga
     5341 tgagcgcgtc cccctctccc cctggaacgc caccggacgc gaagtacatg cctcccatct
     5401 cgtcagggaa tacaccgatg ctgtcgtaaa aaaattccct gaagggatcg gggaagggat
     5461 cacggttgcc gtagaccccg ggtgcggcgc cgcctgcgca accacccccg agatcctcac
     5521 ccgccttgga tgcagggtgg tgaccctcaa cagccagatg gatggcacct tcccgggcag
     5581 gctccccgaa ccctctcctg agggactccg tcctctcggg aacctggtcc gctcaaccgg
     5641 agctgcgctc gggatagccc atgacgggga tgccgaccgg gcggtctttc tcgatgagag
     5701 aggagaattt atcgaagaga accaggagtt tggcatcatc gcaggccagg tctgccggga
     5761 ccggaagggc accgtcgtga tgccggtctc cacctcccgc ctggtcgagg agatcgcccg
     5821 ccgcaacggg tgcgatgtcc agtacactcc ggtgggtagc atctatgttg cccgggcaat
     5881 gcgtgccctc gaagcagccc acagacccgt ggccttcgga ggagaaggga atggcgggct
     5941 catctacccg gatcaccagt actgccgtga cgggggcatg accgccgcga tgatggtcgc
     6001 catccttgcc agggaacacg gtcctgcctc tgaccttgtg gctgccctcc cctcattcac
     6061 cctcatcaag gagaagatca ggggggcgga cccttcggcc atgatcagga acctctgtac
     6121 tgcatttgcc ggggaccaca tcgaccggac cgatgggatc cggatcaacc gggatgagag
     6181 ctgggcactg atccgccctt c
//
LOCUS       DAOOCO010000112         6162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_11984_length_6162_cov_5.145898, whole genome shotgun sequence.
ACCESSION   DAOOCO010000112 DAOOCO010000000
VERSION     DAOOCO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11984_length_6162_cov_5.145898"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /locus_tag="PK154_07040"
     CDS             complement(<1..2344)
                     /locus_tag="PK154_07040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HPW10854.1"
                     /translation="MPSPKRIGKIEFGLLSPKEIRTMSVRKIIWADTYDDDGFPYPQG
                     LMDLNLGVIDPGLRCKTCDQKASECPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRSC
                     SRLLLSPEEIEKVVGTEDDLTGDVLSEKDIKKERVCPHCGEQQLKINFEKPTTFSEIL
                     IEDGKKVEHKLTPADIRARLEKIPDEDLKHLGINPEVARPEWTVLTVLPVPPVTMRPS
                     IILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTTYLDNE
                     VAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSARTVISPDPNLSVIEVG
                     IPLAVANEMSIPVQVTPYNIDDLKQMVLNGPVRTSLTHPCGANYVIRPDKRRLRLAEG
                     NLETVAEQLEPGWTVERQIRDGDIVLFNRQPSLHRMSIMAHRVKVMDGRTFRLNPAVC
                     PPYNADFDGDEMNLHIPQTEEARAEAAILVAVEENIRSPRFGGPIIGGIHDHISGIFL
                     LTNQIRWFTKSEALYLLKDIGVEKLPEPGMQKDGVPFWSNKQVFSTILPQELNMVFKA
                     SSCQNCETCKREGCERDAYVRIIDGTLESGTIDKKAIGAFDGQIVQRVIRQYGMKRAA
                     GFIDDITHLAIRSIMLDGFSFGIDDEDLSRTEYGQIDEVLSNAMLDVQRRINIYEDGQ
                     LEPMPGRTPDETLEMQIMQVLGKARDRTGEIAGRHLGLGNSAVVMAVSGARGSMLNLT
                     QMAGCVGQQSVRGERIMRGYDERTLPHFKKGDRGSDAHGFI"
     gene            complement(2360..4174)
                     /gene="rpoB"
                     /locus_tag="PK154_07045"
     CDS             complement(2360..4174)
                     /gene="rpoB"
                     /locus_tag="PK154_07045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HPW10855.1"
                     /translation="MKKARVFVDGALIGLIDDPKTFVTQIRNMRRQGALSTEVNISHK
                     EFNNDVVIHTDRGRARRPLIVLQNGKPLMSADEIEKLSRKEINFYYFIRKGMIELIDA
                     EEEEDLFVAMNPDQITPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGTSNMKLRPDTRGHLLHYVQRPIVHTQTSDLIGSDDRPGGQNFVVAILSFEGYNI
                     EDALIFNKASIDRGLGRSHFFRTYEGEERRYPGGQVDRIEIPDEEVTGAHGAESYKNL
                     DDDGVINPETIVNEKDVLIGKTSPPRFLEEPTSDLITVEKRRDTSVTMRSNERGIVDT
                     VIITEGENSSRLVKVRTRDLRIPEVGDKFASRHGQKGVIGLIASQEDMPFAESGLTPD
                     LVINPHAIPSRMTIGHMLEMIGGKVGSLEGHRINATAFSGAQESALRSSLKELGFSHT
                     GREVMYDGITGKRFMADIYVGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVQEYVCAHCGMVAMLDRKANTTRCLS
                     CGGETDIYSVEMSYAFKLLLDEMKSMGIAPRMKLEDVV"
     gene            complement(4171..5748)
                     /locus_tag="PK154_07050"
     CDS             complement(4171..5748)
                     /locus_tag="PK154_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HPW10856.1"
                     /translation="MAGLISLIDRSVLSKAYFSREHVARHQLDSFNNFLQANLQKVVN
                     EQKIIETDIEIRGKSSEPVWVELGKIEVKKPLVREADGSQGDLFPSEARLRNLTYAAP
                     IMLELTLVQGEERQEPVVTTIGQLPIMVGSASCNLYGMSDTERISHGEDPFDPGGYFI
                     VNGTERVLMTLEDLASNKIMTEFTERYNERIYVAKVFSQFRGYRALVVVERNRKNLLE
                     VSFPSVAGHLRFVDLMRSLGLVSDHEIVNAVSTDEDILTFMMQNLEESECDSLEGGVM
                     YVGKKLAPNQTRDYQRKRAEFVLDNYLLPHLNYLMPVTLKEGDEGYQEAVAEVRLAKA
                     HFLGRMAEACFDLVLSKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDIKYQLE
                     RASMRHRDLSIGTAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRIDHMSVLSHLRR
                     VISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISRGVPDEDEV
                     IRMLYTMGVEHIRGDVP"
     gene            complement(5748..5999)
                     /locus_tag="PK154_07055"
     CDS             complement(5748..5999)
                     /locus_tag="PK154_07055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HPW10857.1"
                     /translation="MSTRLNVLDHAMVPDHQIMTEEELTGLLSLYKITSEQLPRIYHD
                     DPAVKTIGAKPGDVIRIIRTSHTAGRAESYRLVIRRPKK"
     gene            complement(6079..6152)
                     /locus_tag="PK154_07060"
     tRNA            complement(6079..6152)
                     /locus_tag="PK154_07060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6116..6118),aa:Lys,seq:ttt)
BASE COUNT         1367 a         1677 c         1525 g         1593 t
ORIGIN      
        1 tgatgaaacc atgggcgtct gatccacggt cgcccttctt gaaatgaggg agggtacgtt
       61 catcgtatcc acgcatgata cgttcgccac gtactgactg ctgcccaacg catccggcca
      121 tctgggtcag gttgagcatc gagccccttg caccactcac ggccattact acggcactgt
      181 tgccaaggcc aagatgccgg cctgcaattt caccagtacg atcacgggcc ttcccaagga
      241 cctgcatgat ctgcatttca agtgtctcat cgggtgtcct gccgggcatc ggttccagct
      301 gcccgtcctc gtagatattg atgcgcctct ggacatccag cattgcattc gagagaactt
      361 catcgatctg gccgtattcc gttcgtgaga gatcttcatc atcaatcccg aaagagaacc
      421 cgtccagcat gattgagcgg attgcgaggt gcgtgatatc atcgataaat ccagcagccc
      481 gcttcatccc atactgcctg atgacacgtt gaacaatctg gccatcaaaa gcaccgattg
      541 ctttcttgtc gatggtccct gattcgagtg tgccatcgat gattcgaaca taggcatctc
      601 gctcgcaacc ctcccttttg caggtttcac agttctggca ggaactcgcc ttgaagacca
      661 tgttcagctc ttggggcagt atcgtggaaa atacctgctt gttgctccaa aacggtactc
      721 cgtctttttg cattccaggt tcagggagtt tctcaacacc tatatcttta aggagataca
      781 gggcctcgct cttcgtgaac catcggatct ggttggttag caggaatatc ccggagatat
      841 ggtcatggat tccgccgata atagggcctc cgaagcgagg agagcggata ttctcctcca
      901 cagcaacaag gatagcggcc tccgctcttg cttcttcggt ctgagggatg tggaggttca
      961 tctcatcccc gtcaaaatcc gcattatagg gcggacacac ggcaggatta aggcggaatg
     1021 tccttccgtc catgaccttc acacggtggg ccatgatgct catcctgtgg agagaaggtt
     1081 gccggttgaa gaggacgatg tccccatccc ttatctgtct ctccacagtc catccaggtt
     1141 cgagctgctc ggcaaccgtt tcaaggtttc cttcagcgag ccgaagccgc cgtttatcag
     1201 gtcttatcac ataattggct ccgcagggat gggtaaggct cgtcctgact gggccattca
     1261 gtaccatctg tttcaggtcg tcgatattgt aaggggtgac ctggacaggt atggacatct
     1321 cgtttgcaac ggcgaggggg atgccaacct caatgacgct caggttcgga tcaggggaga
     1381 tgacagtacg ggctgagaaa ttgacccgtt ttccagagag cgagcccctg aaccgcccgt
     1441 cttttccctt gagtcgttgt gagagagttt ttaatggtct cccactccgg tggcgggcag
     1501 gaggacaccc tgcaacttca ttatcaagat aggtggtgac atggtattgg agcagttccc
     1561 agagatcctc gatgatgagc tgaggtgcac cggcatcctg gttctccttg aaccgctggt
     1621 tgatacggat gatgtccacc agtttgtgag tgagatcatc ctcggaacgt tgtccattct
     1681 caaggataat ggatggcctc atagtgacag ggggaaccgg cagcacggta agaacagtcc
     1741 actcaggacg ggcaacttcc ggatttatcc cgagatgctt gaggtcttca tcgggtatct
     1801 tctcaagcct tgccctgata tcagcagggg ttagtttgtg ctcaaccttc ttgccatcct
     1861 cgattagaat ctcggagaat gtggtcggtt tctcaaaatt gatcttgagc tgctgttcac
     1921 cgcaatgcgg acagacgcgt tcttttttga tatccttttc ggagaggaca tctcccgtga
     1981 gatcgtcctc ggtcccaacc actttttcga tctcttcagg ggacaggagc agcctgctgc
     2041 aactccggca ggtgacacgg aggagttttc gtatcagcct cgtgtacccg acatggataa
     2101 ctggtttggc aagttcaatg tgaccaaaat gtcctgggca ttcgctcgcc ttctggtcac
     2161 atgtcttgca ccggagcccg gggtcaatta cacccaggtt gagatccatc aatccctgcg
     2221 gataggggaa accatcgtca tcataggtat ctgcccagat gatcttccga acactcatgg
     2281 tgcggatctc ttttggcgaa agcaggccaa attctatttt tcctatacgt tttggacttg
     2341 gcataacgac ctgttcctcc tacaccacat cttcaagctt catcctggga gcaatgccca
     2401 tactcttcat ttcatcgagc aacagtttga aagcgtacga catctcgacg gagtagatgt
     2461 cagtctcacc tccgcatgag aggcaacggg tggtattggc cttgcggtcg agcatggcta
     2521 ccatgccgca atgggcacac acatactcct gaaccttgtc tgattcgtca agaagccgct
     2581 ccttgagtgc cattgccgct ccgtgaccaa tcatcacatc acgctccatc tcaccaaatc
     2641 tgagacctcc ttcacgtgcc cttccctcag tgggctgccg cgtgagcacc tgtacagggc
     2701 ctctcgatcg ggcatgcatc ttacttgaaa ccatgtggta gagcttctgg taatagatga
     2761 ccccgacata gatatcagcc ataaaccgct tccctgtgat accatcgtac atcacttcac
     2821 ggccggtgtg ggagaatcca agctccttca gcgaggaccg gagagccgac tcctgtgctc
     2881 ccgagaatgc agtagcattg attcggtgac cttcaaggga cccgactttg cccccgatca
     2941 tttcaagcat atgcccaatg gtcatgcgac tcgggatggc atgagggttg atcaccaggt
     3001 caggggtcag gcctgattcg gcaaatggca tatcctcctg ggaggcgatg aggcctataa
     3061 cccccttctg tccatgcctg gaagcgaatt tgtcacccac ttcaggaatg cgcaggtccc
     3121 gggttcttac tttgaccagc cgcgagctgt tttcgccttc ggtaatgatg acggtatcaa
     3181 caatgcccct ctcattgctt cgcatcgtga ccgatgtgtc cctccttttt tcgacggtga
     3241 tgagatcgct cgttggctct tcgagaaatc gtggagggga ggtcttcccg atcaggacat
     3301 ctttttcatt tactatggtt tcaggattga taaccccatc atcatcgagg tttttgtagc
     3361 tctccgcacc atgagctccg gttacttctt catccggaat ctcgatccgg tctacctgcc
     3421 ccccaggata cctccgctcc tcaccctcat atgtccggaa gaagtgggat ctccccagac
     3481 cgcgatcgat cgaggctttg ttgaagatca gggcgtcttc gatattatat ccttcaaaac
     3541 taaggatcgc aacaacgaag ttctgtcctc cgggacgatc atccgatccg atcagatcag
     3601 aagtctgggt gtggacaatg ggcctctgga cataatgaag cagatgaccc cgggtatctg
     3661 gcctgagctt catgtttgat gtgccaaagc ccaaagcttg cttcaccatc cccgcaccca
     3721 ttgtgacacg agggcttgca ttgtgctcag ggaacggtac atgggcagca ccgattccaa
     3781 gtatgaggga gggatcgatc tcaagatgcg tgtgctctgg cgtgatctga tcggggttca
     3841 tggcgacaaa gagatcttcc tcttcctcgg catcgatcaa ctctatcatt cccttccgta
     3901 tgaagtaata gaagttgatc tcctttcgac tgagcttctc tatctcatct gcactcatca
     3961 gaggttttcc attctggagt acgatgagag ggcgccttgc cctcccccga tcagtatgga
     4021 tcacaacatc gttattgaat tctttatggg agatgtttac ttcggttgat agggctccct
     4081 gtcgtcgcat attcctgatc tgagtgacaa atgtttttgg atcatcgatg agaccgataa
     4141 gagctccatc aacgaagact cgtgcctttt tcatggtaca tcacccctga tatgctctac
     4201 ccccatggtg tagagcatgc ggatcacttc atcctcatct ggtaccccac gactgatttc
     4261 caccatctgg gcaaagttct tgacaagccc acagttgggt ccttccggag tttcactcgg
     4321 acaaatcctt ccccactgag taggatggag atctcgcgcc tcaaagtgag gctgcgaccg
     4381 tgagagagga gatataacgc ggcgaaggtg tgagagaacg ctcatgtggt caattcgatc
     4441 cagcagctgc gatacgcctg tccttccccc tacccagttt cctgttgcga gaggatggag
     4501 cagtcgctcg gttaatacat ctgctctcac tgccgtccca atagagagat cgcggtgacg
     4561 catacttgcc cgctcgagct ggtatttgat gtcacgtgtg agacggttca gggagatcct
     4621 gaagagatct tccatgagat cccccgcaag cttcagccgc ttgtttgagt aatgatcttt
     4681 gtcatcaatc cgtctcttgg aaaggacaag atcaaaacag gcctctgcca ttctcccgag
     4741 gaaatgggcc tttgccagtc tcacttccgc aacggcctcc tgatagcctt catcaccttc
     4801 tttgagggtg accggcatca ggtaattgag atgcgggagg aggtagttgt caaggacaaa
     4861 ttctgcccgt ttccgctggt aatctcttgt ctggttaggg gcaagcttct tgccgacata
     4921 catgacaccc ccctcgaggc tgtcgcattc gctctcttca aggttctgca tcatgaaggt
     4981 gaggatatcc tcgtcggtag aaacagcatt cacgatctcg tgatcgctga caagcccgag
     5041 tgagcgcatc aggtccacaa acctgagatg cccggcaaca gagggaaatg agacctcaag
     5101 caggtttttc ctgttccttt cgaccacgac aagggcacga taacccctga actgggaaaa
     5161 cacctttgca acgtaaatgc gttcgttata gcgctcagtg aattccgtca tgatcttgtt
     5221 tgaagcaaga tcctcaagcg tcatcaggac ccgctcagtc ccgttcacaa taaaatagcc
     5281 gccggggtcg aatggatctt cgccgtgtga aattcgctct gtgtcactca ttccataaag
     5341 attacaggat gcggacccaa ccatgattgg aagttggccg atagtagtta ctaccggctc
     5401 ctgccgttcc tcaccctgta caagggtcaa ctcgagcatg atgggtgcag catacgtgag
     5461 atttcgcaat cgtgcctcgc tagggaaaag gtcaccctgg gaaccatccg cttctctcac
     5521 cagtggtttt ttgacctcta tcttacccag ttcgacccag acaggttcgc tgcttttacc
     5581 acgaatttca atgtctgttt caatgatctt ctgttcattg accactttct gaagatttgc
     5641 ctggagaaaa ttgttgaatg agtcaagctg gtggcgcgca acatgctcac gtgaaaaata
     5701 tgcttttgat aatacgctcc ggtctatcag agatatcagc cccgccatta tttttttggt
     5761 cgccttatga caagtcggta tgactcggct cttccggctg tatgactggt tcggatgatc
     5821 cggatgacat ccccgggttt agccccgatg gtttttacag caggatcatc atggtaaatt
     5881 ctcggcaatt gttcgctggt aattttataa agagagagca acccggtcag ctcttcttcg
     5941 gtcatgatct ggtgatcagg caccatcgca tgatccagca cattcaatcg tgtgctcaca
     6001 gtaattcccc cctgattacg aatgcattca tcgtcgtcag atacctcatt tatgaaaaat
     6061 ccgcccgctt cgcggcagcg ggcccggagg gatttgaacc cccgaccacc tggttaaaag
     6121 ccaggcgctc tacctaactg agctacgagc ccaccctgtc gc
//
LOCUS       DAOOCO010000113         6151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12001_length_6151_cov_5.548556, whole genome shotgun sequence.
ACCESSION   DAOOCO010000113 DAOOCO010000000
VERSION     DAOOCO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12001_length_6151_cov_5.548556"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..847
                     /locus_tag="PK154_07065"
     CDS             74..847
                     /locus_tag="PK154_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPW10858.1"
                     /translation="MRISPYFSSSSKVWDDIGWVEGIATTGYEGWEIVADGSYRLDNP
                     ENVAKIDDVIGSLTLGISVHAPYADLNLATLNYPIWRESIRQVCTCVRQAAEWTDRVT
                     IHPGYVSPVGKIMPDRTWQQQKEALIEIGRVASDHSVVACLENMIGIREFLCRMPEEL
                     AGMVDGIEGIGMTIDIGHANTVGKVREFLPYLSQASHLHVHDNHGVHDEHLHLGAGTI
                     DWDLVGRCIARDYSGIVVVEGRSLEEGKTSLSTVRGWQA"
     gene            844..1788
                     /locus_tag="PK154_07070"
     CDS             844..1788
                     /locus_tag="PK154_07070"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPW10859.1"
                     /translation="MSGETLQVYFLGTAGALPTPYRNPPCIMVRRGSDTLLFDCGEGA
                     QQQMMRSRTGFLVDAIFVTHWHADHFLGIAGLVQTLSFMGRADPLPIYGPAWVHEAVS
                     AIRALSRFSLKFTLSPVQLAPGSAIRFNGYSVRAFATDHGMESIGYVLAEDPRPGRFN
                     RERAIALGVPPGPLFGRIQRGETVCIEKDGRNLPVSPADVMGPPRPGRSIVYTGDTRP
                     VPHRIAELITAPDLLIHDATYDDECRGRADDVYHATAGEAGEAARVLGARILALVHIS
                     SRYTTTAGHVRDATRVFGGTVIAPSDLELLELPFREDD"
     gene            1946..3787
                     /locus_tag="PK154_07075"
     CDS             1946..3787
                     /locus_tag="PK154_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPW10860.1"
                     /translation="MQDPDDVTVYRMGPGCDLNDVEEGKVYIGRVQGFANFGTFVQLN
                     DRVKGLCHKSNIKTQHKERETILVRVKNIRSNGNIDLEEVTYPVYTVEMVEKKSTAVR
                     LAELGNKVGRIVSIEGEIAQIKQTSGPTIFTIVDESGTENAAAFVEAGVRAYPEAELE
                     DVVRITGEVMLRNNQLQIEARSLTILTGEEGEAVRERIAQALDQRSEPPDIPLLIKSD
                     VLEQLRPAMRKVAKVIRKAVFSSQPIIVRHHADADGICSAVAIEQAVTSLIRESGGDF
                     DADYYLFKRSPSKAPFYEIEDITRDLDFALKDHARFGQKLPLVLLTDNGSTEEDLPSL
                     KMAQIYEIPIVVVDHHHPDEIVDQYLVGHVNPYHVGGDYGITAGMLGTEVARLINPSV
                     EQHIRHLPAIAGAGDRSEAPERKRYHELVQDAYTEQDCKDIALSLDYEQFWLRFNDGR
                     ELIKDILNLKGATDRHKKMIPLLVEGANALIEDQMNACMPHVRHQTLANGAELFQIDV
                     EIHAHKFTFPPPGKTSGEVHDRLCKQYAGSPVVTIGFGPDFAVLRSKGVMMNIPKMVR
                     ELRDEIPGGGISGGGHLVVGSIKFVEGMRESVLEGLIEKISRVPAGL"
     gene            4621..4869
                     /locus_tag="PK154_07080"
     CDS             4621..4869
                     /locus_tag="PK154_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10861.1"
                     /translation="MTSGTIREKYNETQHRIVHYLLGGLSRGKHYFKSKYIAKELGLS
                     AKEVGTNMAILSEICQELAIIKWSYSNSTTWMVTSKGI"
     gene            4891..5079
                     /locus_tag="PK154_07085"
     CDS             4891..5079
                     /locus_tag="PK154_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10862.1"
                     /translation="MEPIAEIETVIEFVAHINEQKDCLMSQDNTQNNPGCLWFNIDVP
                     KGHGLKAGDKVRVIVQKL"
     gene            5438..5511
                     /locus_tag="PK154_07090"
     tRNA            5438..5511
                     /locus_tag="PK154_07090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5473..5475,aa:Pro,seq:ggg)
     gene            5546..>6151
                     /locus_tag="PK154_07095"
     CDS             5546..>6151
                     /locus_tag="PK154_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="damage-control phosphatase ARMT1 family protein"
                     /protein_id="HPW10863.1"
                     /translation="MKFDDRCVDCLLSRVMLECQLCGASGLRSEEVTAACASLLEEIR
                     DQPLLHPQIASAMHRRAYTMLANDDPFFALKEESDRIAREVCRAVRSRLSGFYDYTLA
                     SIIGNTFDYGVKGHQVTGDFLGYFEQEFAAGLAIDDTDRIREKIDRVVYFTDNCGEIV
                     FDRLLLEYLHRQGSVITLAVRDMPILNDATMAEARALHLDLF"
BASE COUNT         1284 a         1836 c         1812 g         1219 t
ORIGIN      
        1 tcatgaacga cggcagcatg gaggagttcg aggagcaggt cagggatctc ctctctgaga
       61 tcggggagga gccatgcgaa taagccccta tttctcatca tcgtccaaag tatgggatga
      121 catcgggtgg gtcgagggga tcgcaacgac cggatacgag ggctgggaga tcgtggccga
      181 tggatcctac cggctcgaca atcccgaaaa cgtcgcgaag atcgatgatg tcatcggctc
      241 gctcacgctc gggatcagcg ttcatgcccc ctatgccgac ctgaaccttg ccaccctcaa
      301 ctacccgatc tggcgggaat ccattcgcca ggtctgcacc tgcgtccggc aggcagccga
      361 gtggaccgac cgcgtcacca ttcatcccgg gtacgtctcc ccggtcggga agatcatgcc
      421 cgatcgcacc tggcagcagc agaaggaggc gctcatcgag attggcagag tcgcatccga
      481 tcactcggtg gtggcatgcc tcgagaacat gatcgggatc cgggagttcc tttgcaggat
      541 gcccgaggag ctcgccggga tggtggacgg gatcgagggg atcgggatga ccatcgacat
      601 cggccatgcc aacacggtgg gcaaggtgcg ggagttcctc ccgtacctct cccaggcctc
      661 gcacctccat gtccatgaca accatggcgt ccatgacgag caccttcacc tcggcgccgg
      721 gacgatcgac tgggatctgg tcgggagatg catcgcccgg gattattccg ggatcgtggt
      781 ggtcgaagga cggtccctgg aagagggaaa aaccagcctc tcgaccgtga ggggctggca
      841 ggcatgagcg gggagaccct ccaggtctac ttcctcggga cggccggggc gctgccgacc
      901 ccgtaccgga acccgccctg catcatggtc aggaggggat cagacaccct cctcttcgac
      961 tgcggggagg gggcccagca gcagatgatg cggtcccgca ccgggtttct cgtggatgcc
     1021 atcttcgtca cccactggca tgccgatcac ttcctcggga ttgcggggct cgtccagacc
     1081 ctctccttca tggggagggc cgacccgctc ccgatctacg gcccggcatg ggtgcacgag
     1141 gcggtctccg cgatccgggc cctctcccgg ttcagcctca aattcacgct ctccccggtc
     1201 cagctggcgc ccggctctgc gatccggttc aacggctact cggtcagggc atttgccacc
     1261 gatcacggga tggagagcat cgggtacgtc cttgcagagg acccccggcc agggcggttc
     1321 aaccgggagc gggcgatcgc actcggggtc ccccccggcc ctctcttcgg caggatccag
     1381 cgcggggaga cggtctgcat cgagaaggat ggcaggaatc tccctgtcag ccccgcggac
     1441 gtgatgggtc ccccccggcc cggacggagc atcgtctaca ccggggatac ccggccggtc
     1501 ccccaccgga tcgcggagct gatcaccgcc cccgacctcc tgatccatga tgcgacctat
     1561 gatgacgagt gccggggccg ggctgatgat gtctaccatg ccaccgcggg agaggcaggg
     1621 gaggctgccc gggtgctcgg tgcccggatc ctggccctgg tccatatcag ctcgcggtac
     1681 accacgacgg cagggcatgt cagggacgct acccgggtct ttggggggac cgtgatcgcc
     1741 ccctcagacc ttgaactgct cgaactgccg ttccgcgaag atgactgagc gttggaaggc
     1801 gatcggtacg gatcctttcc atcgatcgat acggatccat gaatgatatg aacaacagtt
     1861 tccatagaat gataagaacg accggcgcat ggttaattac tggagtacca caacgtgcac
     1921 caaacgtatc aataccggtg aaagtatgca agatccagat gacgtaaccg tatatcgcat
     1981 gggccctggc tgcgacctga atgatgttga ggaagggaag gtctatatcg ggcgtgtcca
     2041 ggggttcgca aattttggca cctttgtcca gctgaatgac cgggtgaagg ggctctgcca
     2101 taaaagtaat atcaagaccc agcacaagga gcgcgagacg atcctcgtgc gtgtgaagaa
     2161 tatccggagc aacggcaaca tcgacctcga ggaggtaacc tacccggtgt acaccgtcga
     2221 gatggtggag aagaagtcca ccgcggtccg gctcgccgag ctcggcaaca aggtggggag
     2281 gatcgtcagc attgagggcg agatcgccca gatcaagcag acgagcgggc cgaccatctt
     2341 caccatcgtg gacgagtcag ggaccgagaa tgccgctgca ttcgtcgaag ccggggtccg
     2401 ggcatacccg gaagccgaac tggaggacgt ggtcaggatc accggcgagg tgatgctccg
     2461 gaacaaccag ctccagatcg aggcgcggtc gcttaccatc ctcactggtg aagaggggga
     2521 ggccgtccgt gaacggatcg cacaggccct cgaccagcgg tcagaaccgc ccgacatccc
     2581 gctgctgatc aaaagcgatg tcctcgagca gctccgcccg gccatgagaa aggtggccaa
     2641 ggtgatcagg aaggcagtct tctcctccca gccgatcatc gtccggcacc atgccgatgc
     2701 agacgggatc tgttcggcgg tcgcgatcga gcaggcagtc acctccctga tccgggagtc
     2761 aggtggcgac ttcgacgccg attactacct cttcaaacga tccccctcga aagccccctt
     2821 ctacgagatt gaggacatca cccgggacct cgatttcgcc ctgaaggatc atgccaggtt
     2881 cgggcagaag ctcccgctcg tcctcctgac cgacaacggc tcgaccgagg aggacctccc
     2941 gtcgctgaag atggcccaga tctacgagat ccccatcgtc gtggtggacc atcaccaccc
     3001 ggacgagatc gtcgaccagt acctcgtcgg gcatgtcaac ccgtaccatg tcggcgggga
     3061 ttacgggatc acggcaggga tgctcgggac cgaggtcgca cggctgatca atccatcggt
     3121 ggagcagcac atccggcacc ttcctgccat cgccggggct ggcgaccgga gcgaggcacc
     3181 cgagcggaaa cgctaccacg agctcgtcca ggatgcctac accgagcagg actgcaagga
     3241 tatcgctctc tccctggact acgagcagtt ctggctccgg ttcaacgacg gccgtgaact
     3301 gatcaaggac atcctgaacc tcaagggagc aacggaccgc cacaagaaga tgatcccgct
     3361 cctcgtggag ggtgccaatg cgctgatcga ggaccagatg aatgcctgca tgccccatgt
     3421 ccggcaccag acgctggcaa acggggccga gctcttccag atcgatgtcg agatccatgc
     3481 ccacaagttc accttcccgc ctccgggaaa gacctcgggc gaggtgcatg accggctctg
     3541 caagcagtat gccgggtcgc cggtggtcac catcggtttc gggcccgact tcgccgtcct
     3601 ccgctccaaa ggcgtgatga tgaatatccc gaagatggtc cgcgagctgc gggacgagat
     3661 ccccggcgga gggatcagcg ggggagggca cctcgtggtc gggagcatca agttcgtgga
     3721 agggatgcgt gagagcgtgc tggaggggct gatcgagaag atctcgcggg tccctgccgg
     3781 gctctgatcc ctcccgtttc cattcttctg cggtacaacc ttctcctgta attcttattc
     3841 ctgtctcgac ctttgattcg tacggtacaa ccttctcctg taattttcat tcctgtcttg
     3901 acctttgatt catgcggtac gccattctcc tgtaattttc attcctgtct tgacctttga
     3961 ttcgtgcggt acaacctctt cctgatcctg tcctctcctc tcatttccag gagtagatac
     4021 gagatctcca tgaactaagc aacgcttatc gcatcgcggg ggacgccaca gcggcggggg
     4081 tgagccgaag gcggaccccc tggagcgtca gtagtgacat accggggtaa gagttcaccc
     4141 atctctgcag cgcttatcgc atcgcggggg acgccacagc ggcgggggtg agccggaggc
     4201 gaaaccccct ggagcgtcag caggaacatg ccggtgtgag atctcccctt ccgatcaact
     4261 ccctatcgca tcgcggggga cgccatagcg gccgggggaa agccggaggc agaacccccg
     4321 ggagcgccag gcaagggata caactccttt cgagcagacg agaaccaaga ccttttcctg
     4381 cccttgcccc ctttcctttc cagcaaactg ggagaggcac ggatcccggc atgcaggggt
     4441 ccggatatct gctttcaggg gccttgcatt aaccttttgg ttagtgcgaa taatttcgca
     4501 gttaaattga cgaatcaaag aaaggacgta tttaaatgct ttgctgttgc catgcgagat
     4561 aaaaaagtat atatacatta tcatgacaag tagatctcac attgctgagg tgactcacat
     4621 atgacatcgg gaacaatccg agaaaaatac aatgaaaccc agcacaggat cgtccactat
     4681 ctgctggggg gtctctcaag aggcaaacac tacttcaagt ccaagtacat cgcaaaggag
     4741 ctcgggctct cggcaaagga ggtcggcacc aacatggcta tcctctcaga gatatgccag
     4801 gagcttgcga tcatcaagtg gagctattcg aacagcacga cctggatggt aacatccaaa
     4861 gggatctgag ttttaggatc actccttttt atggaaccga ttgccgagat tgagacggtc
     4921 atcgaattcg tggcccatat caatgagcag aaagactgcc tgatgtccca ggacaacacc
     4981 cagaacaatc ccgggtgcct ctggttcaat atcgatgtgc cgaaaggcca tggtctcaag
     5041 gcaggggaca aggtcagggt catcgtccag aaactctgat cctctccccc ccgttttgga
     5101 gtcttcgtct tagtttagat catcattggt gagatctcac tctatgggat ctcactctat
     5161 gggatctact cttttagggt ctcaatcctt gggatctcaa tccttcggat cttaccttca
     5221 gcatggtatc tgcagggatc tcactcttgg aggtttctgt gctagggatc tctttttgta
     5281 aaaccatcct acaggcctct ccccctcata tcccgctctg atcctgtccc ctgccatgcc
     5341 gggacggggc tttcctcacg cccgggaacc ccgggggtgc ctcccccggg gtataaagag
     5401 aaattacata agcgatcctt gccaatacca taagagcggg gccatagggt agcctggcca
     5461 tcctaggaga ctgggggtct tctgacctgc gttcaaatcg cagtggcccc attcatgtgt
     5521 tttttaaaag cggtgttcgt ggcccatgaa attcgatgac cgttgtgttg actgcctcct
     5581 ctcccgtgtc atgctcgaat gccagctctg cggtgcatcg ggtttgcgat cggaggaagt
     5641 gaccgcggca tgcgcctccc tcctcgaaga gatccgcgac cagccgctcc ttcatcccca
     5701 gatcgcgagt gccatgcacc ggagggcgta cacgatgctt gcgaacgacg acccgttctt
     5761 tgcgttgaag gaggagagcg accgcatcgc ccgcgaggtc tgccgtgccg tccggagccg
     5821 gctctccggg ttttatgact atacgcttgc gagcatcatc gggaacacct ttgactacgg
     5881 ggtcaagggc caccaggtga caggggattt tctcggctac ttcgagcagg aatttgctgc
     5941 cgggctggcc atcgatgaca cggaccggat ccgtgagaag atagaccggg ttgtctactt
     6001 caccgacaac tgcggggaga ttgtctttga ccgcctgctc ctcgagtacc tgcaccggca
     6061 gggatcagtt atcacccttg cagtccgcga catgcctatc ctgaacgatg ccacgatggc
     6121 agaggcccga gcgctgcatc tcgacttgtt t
//
LOCUS       DAOOCO010000114         6143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12031_length_6143_cov_4.421321, whole genome shotgun sequence.
ACCESSION   DAOOCO010000114 DAOOCO010000000
VERSION     DAOOCO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_6143_cov_4.421321"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK154_07100"
     CDS             <1..233
                     /locus_tag="PK154_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10864.1"
                     /translation="IACPGAQHLARTAAEVEQTSPRFQAQRGAERGEMFRGERVMDAV
                     STFSYGEDTWDVHCHKLLFLRWVITTFRDGRR"
     gene            426..626
                     /locus_tag="PK154_07105"
     CDS             426..626
                     /locus_tag="PK154_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10865.1"
                     /translation="MIDPLMVKQLAMARRWGEAANREGGQSPPRSPARHGIRGNTHVP
                     SSVLNNSGIADLLSGFLAESGL"
     gene            complement(806..1786)
                     /locus_tag="PK154_07110"
     CDS             complement(806..1786)
                     /locus_tag="PK154_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10866.1"
                     /translation="MVRYYIKIGNCLGFNHEHIIEVTPIDVIIKGEEEAKKEASKKDE
                     TPQKEEENPFDKNKFYTTVYDCRSKGKTVLATFKGDQVYRDDNPPDPLPVVTDVRELT
                     EGDDLDWLTHHREQQQGLSAHFIQKAEGYQNLWTAVFTIYTGVLVLFGFMEAGGSTLT
                     QLEWYLKALFLLPILAWIAGIYFFFKVFEPTVGKMTPNSPAEIRENYTTANVAKANNY
                     RRGIACFSVGILFIAVALIGGIFFTSHSDTPGENVIFIIKDEFVEKVQEIPISMIPET
                     NMTTVVQLYNTTESGYRIGLEGGTIVELDKNWVTNIIRVSNTTEQGSQTA"
     gene            complement(1933..2091)
                     /locus_tag="PK154_07115"
     CDS             complement(1933..2091)
                     /locus_tag="PK154_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10867.1"
                     /translation="MNIALFLAGLCIALTAAALLFFDIIEPGLAAVIGIVGIGLIGAS
                     GMSHIRRL"
     gene            complement(2287..2466)
                     /locus_tag="PK154_07120"
     CDS             complement(2287..2466)
                     /locus_tag="PK154_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10868.1"
                     /translation="MRQFLSLFLVVAAVIMGISAADTAKEFLAWILPLLISWAQMPDI
                     SALINKVSVKGNGLI"
     gene            complement(2787..3053)
                     /locus_tag="PK154_07125"
     CDS             complement(2787..3053)
                     /locus_tag="PK154_07125"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HPW10869.1"
                     /translation="MYFVALLKFKKKPDKAFMIETQKLIDADTQKGFRFHAIYWTLGK
                     YDAVAVYEAPDEKEAMKAAMKRVELMDVETYVAIPREEVKKLVE"
     gene            3362..3763
                     /locus_tag="PK154_07130"
     CDS             3362..3763
                     /locus_tag="PK154_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPW10870.1"
                     /translation="MKWIQGEVRTSARIQMVDITGMVAAELEKSGVKTGVCHVYVPHT
                     TAGLTINENADPDVTRDIIATLNRLVPATGDYRHFEGNSDAHVKASLMGFSAIVPVFD
                     GHLALGTWQALYFCEFDGPRHRHVVIGISGE"
     gene            3822..4385
                     /locus_tag="PK154_07135"
     CDS             3822..4385
                     /locus_tag="PK154_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPW10871.1"
                     /translation="MPQLKALSPEQQKELLSRGKARFEKNMNRHNGLLWKNVQAQLEA
                     NPEKMWSLHAMERTGGEPDVVGHDTTTGEYIFYDCSPESPTGRRNVCYDREGQEARKT
                     NIPDNNAIDMAAAMGIELLTEEQYRALQALGDFDMKTSSWVKTPPAIRQLGGALFAHR
                     RYGTVFLFHNSAQSYYAARGFRASLRV"
     gene            complement(4447..5025)
                     /locus_tag="PK154_07140"
     CDS             complement(4447..5025)
                     /locus_tag="PK154_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW10872.1"
                     /translation="MESVEEKAIGIVSRAATGAHQGQTRKDGKTPFIVHPERVAKCVA
                     HYGGNHVGIIAAWLHDVIEDNDEGAAIVREALEETCLPENNLHEIYAIVLALTKNPAI
                     PGKRERFADTLDRIVHAPGQAILVKICDRMDNLIDARDQGQEFLSEYLDLTDDLIAAL
                     SDGAIRYGYSEALEHLKVVREDLGFFYEAKFF"
     gene            complement(5146..>6143)
                     /locus_tag="PK154_07145"
     CDS             complement(5146..>6143)
                     /locus_tag="PK154_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10873.1"
                     /translation="YERVAALAQEAGYPKSMINPYVIPSGLLNLGVFPETPLADSFTI
                     LMRTANFVSKAEGDHYLRDDYYARVWRVTPEEALTLDPFPTPEQRVRTWKSESELYPD
                     LNNGLERLENAILAQTPHVQYESYDSIRWFADSREVLEETDPSSPLYHKFVAGEASDT
                     PYLRTSLSGEAANFTLGNDDMVVVYGVNHAATGLATYSNFGVYGDWVTSSCEDNPWQY
                     VFGCGDRSWNGVASMTNHDFTGSAEEYIPGDPMAPYLYAVKVLRQPPQDKHDKYFVIV
                     PTATSDHPCESPSYCINLTDPIIIGYRAYLNPATGSGADYDDIIHDRAIWFRVRE"
BASE COUNT         1469 a         1623 c         1582 g         1469 t
ORIGIN      
        1 taatcgcgtg cccaggtgca cagcatctcg cccgaaccgc tgccgaggtc gagcagacga
       61 gcccccggtt ccaggcgcaa cgcggcgccg agcgtggcga gatgttccgg ggtgaacggg
      121 ttatggatgc ggtgagcact ttcagttatg gtgaagatac gtgggatgtc cactgtcaca
      181 aactcctttt cctacgttgg gtaataacaa cctttcgtga cgggaggaga taagggtttt
      241 aactctctca ccacttcgag ttgttgcatt ggtgttccgc aatgaaccct gaactgatcc
      301 tttttcaaaa atagtgggaa agcatccgtg tggattccat tctctttcct cagcactcgt
      361 ttgccctgat tccacacatt tccactacca ttgctatgat tctcaccctt ggtaaagggt
      421 gttcaatgat tgatccgctg atggtgaaac agcttgcaat ggcacgacgc tggggggaag
      481 cagcaaaccg ggagggaggt caaagcccgc ctcgttcacc tgcccgacat ggcatccgcg
      541 gcaacacgca cgttccctct tcggttctga acaattccgg gatcgccgat cttctctccg
      601 ggtttcttgc ggagtcgggc ctgtgatccg gacctaacgc gaaaaaggtt catacaatcc
      661 ggcccgtcct ccttccagac ctttacattc gaataaaagg caacggacag gatggtcagg
      721 agcgtaatta tccccctcct ctggaaaggg gaggagtaag accgcttaaa aagggactaa
      781 taggattgtc gtctatcccc cttagttatg cggtttgact gccttgctcg gtggtgtttg
      841 aaacccggat gatattcgtg acccaatttt tatccaattc tacaatagtt cccccctcca
      901 gcccaatccg ataccctgat tccgttgtgt tgtagagctg aacaacagtg gtcatattgg
      961 tttctggaat catactgatg ggaatttcct gtaccttctc cacgaactca tccttgatga
     1021 taaatatgac gttttcccct ggtgtgtcag aatgggatgt gaaaaatatc cccccaataa
     1081 gtgcaactgc gataaaaagg atgccaactg agaaacaagc tattccccgg cgataattat
     1141 tcgcctttgc tacgtttgct gtagtataat tttcccggat ttcagcgggg gaattgggag
     1201 tcatttttcc tacagtaggt tcaaaaacct taaaaaagaa gtaaattcca gcgatccagg
     1261 cgaggattgg caacaggaaa agagctttca gataccattc taactgagtt aaggtgcttc
     1321 ctccagcctc catgaatccg aagagcacga ggacccctgt ataaatcgta aatacagcgg
     1381 tccatagatt ctgatagcct tcagcttttt gaataaagtg cgcacttagc ccttgctgtt
     1441 gctctctatg atgtgttaac caatcaagat catctccctc tgttagctca cgaacgtcgg
     1501 taaccacagg taaagggtca ggggggttat catcgcgata aacttgatct cccttaaagg
     1561 ttgccaacac cgtctttcct ttacttcgac aatcatacac agttgtatag aatttattct
     1621 tatcaaaagg attctcctcc tctttttggg gagtctcgtc ctttttggaa gcctcttttt
     1681 ttgcctcttc ttccccttta attatgacat caattggagt gacctcaata atatgttcat
     1741 gattgaagcc aaggcagttt ccaattttaa tgtagtatcg gaccattgaa ttttatcccc
     1801 tttcagttat atgggccttt taatgttttc aagtatttag aattaatcat cacctatcct
     1861 ctccatcact gtccgaattc gagatcgtca ttccataccc ggcacccggg gatcatggga
     1921 tagcccctct ccttacagcc gcctgatgtg cgacatacct gatgcgccaa tcagtccgat
     1981 gcccacgata ccgatcactg cggcaagtcc gggctcaatg atatcaaaaa agaggagtgc
     2041 agctgcagtt aatgcgatac ataacccggc aagaaagagc gctatgttca tcgaagtgca
     2101 tccacaccct cctttaaaga atgagtgggc ataaatcttt ggtatcctcc cttttctctt
     2161 cctgcacaac accatcagca ccctgatagg tgcgagcagc gttccagaga cagacgggac
     2221 tgccagagat gtgatcgtga gcaggtggga aaaaagtgtg tgcggtggct tggaaaggtt
     2281 cggctatcag ataaggccgt ttccctttac cgaaaccttg ttgataagag cactgatatc
     2341 aggcatctgt gcccaggata tcaacagggg taaaatccac gccaggaact cttttgcggt
     2401 atccgcagcc gatattccca tgataacagc ggcgacgacc aggaaaagag aaagaaattg
     2461 tctcatggta ttgttcctcc ttgtatggta ttgctcttcc ttttggatag aggtaagaag
     2521 gagaggaatc gggatcgatg atactttttt tgaacctcct ccttttttcg cctacatgaa
     2581 ggactataaa ttggagcgat ttgttcgtag tgataacccg tcccggattc cattgaactg
     2641 tcagtgaaaa gagcaagggg tgcgacacgg ttgcagagag ttcacaaaaa atccatcctg
     2701 tgcagggaaa tacgatcgcg aggaagtgag gggagatggg gtgttcccgg agcctgaaga
     2761 gaaaaagtga gtgcccgaat ccccttctac tccaccagtt tcttcacttc ttcccgcggg
     2821 atagccacat aggtctccac atccatgagt tcgaccctct tcatggctgc tttcatcgcc
     2881 tctttttcat caggggcttc atacaccgcc acggcatcgt atttcccgag agtccagtaa
     2941 atagcatgaa atctgaaccc tttctgggta tcggcatcga tcaatttctg cgtctcgatc
     3001 ataaacgctt tatccggctt ctttttgaac ttcagcagtg caacaaaata catcggtcct
     3061 tactcctccc ttttcagggc atggtaccat acgaggctga cgataaaaag gtacctgaca
     3121 tcatccggag aaggataccg tgacaccccc ttgctcgcgt tggaggggat ggagagcaat
     3181 gctcttcttc cttgtcaccc cggccatcca ggagcatggg agggaatgaa tatcatgagg
     3241 atgatcactg atgggagaac gagagaaagt gaggagatgg cgtggatacg aacaatccga
     3301 ttggaatcaa accagcggac ctgcataagg caggcagact ggtggcacag aaggtgagat
     3361 catgaaatgg atacaaggag aagtacgcac gagtgccagg atccagatgg tggatattac
     3421 cgggatggtc gcagctgagc tggaaaagag cggcgtgaaa acaggcgtct gccacgtgta
     3481 cgtaccccat acgacagccg gccttacgat aaatgagaac gccgaccctg acgtcacccg
     3541 tgatatcatt gccaccctca accggctggt gccggcaacg ggcgactatc gccacttcga
     3601 ggggaattcc gatgcccatg tcaaggcatc gctcatgggc ttttccgcga tcgtccctgt
     3661 cttcgacggc cacctggctc tcggaacctg gcaggccctg tacttctgcg agttcgacgg
     3721 accccgtcat cgccacgtgg tgatcgggat cagtggcgag taggagggga tgaacctcac
     3781 cgtcatcgat gattttttaa aacaggggtg aatactggat tatgcctcag ctcaaagcct
     3841 tgtcaccgga acaacagaaa gaactactca gcagaggaaa agcccgtttt gagaagaaca
     3901 tgaaccgcca taacggcctt ctctggaaga acgtgcaggc acagctggaa gccaatcccg
     3961 aaaaaatgtg gtcgctccat gcgatggaac gaacgggagg ggagccggat gtggtcggtc
     4021 atgatacaac gacaggcgaa tacatcttct atgattgctc accggagagt ccgacaggtc
     4081 gcaggaatgt ctgttacgat cgtgaagggc aggaggccag gaagaccaat ataccagaca
     4141 ataacgcaat tgacatggca gcagccatgg gcattgagct gttgacggag gagcaatacc
     4201 gggcgctgca ggcactagga gattttgata tgaaaacatc gagctgggtg aagacccctc
     4261 ccgcgatcag acaactcggc ggggccctct ttgcccaccg acgctacggc acggtctttc
     4321 tcttccacaa cagtgcgcaa tcgtactacg ctgcacgggg atttcgtgcc tcgctcagag
     4381 tctgaaaggg aggattttca cattttacac ggctgggagt ggtcttcccc ttccccttgc
     4441 catagattaa aagaattttg cctcgtaaaa aaacccaagg tcctcccgca cgaccttcag
     4501 gtgctcaagt gcttcagaat agccataacg aatggcacca tcagataagg cagcaatcag
     4561 gtcatcggtc aggtccaggt attcagataa gaactcctgc ccctgatcgc gggcatcgat
     4621 caggttatcc atccggtcac agatcttgac aaggatcgcc tgccctggtg catgaacaat
     4681 ccggtccagc gtatcggcaa accgctcccg tttcccggga attgccggat tcttggtgag
     4741 agccaggacg atggcgtaga tttcatgcag gttgttctcg ggaagacacg tctcttccag
     4801 ggcttcgcgg acgatggcag caccttcgtc attatcctcg atcacgtcgt ggagccaggc
     4861 cgcaatgatg ccgacatggt tgccgccgta atgggcaaca cacttcgcca cccgctcagg
     4921 gtggacaata aagggggttt tcccgtcttt cctggtctga ccctggtggg ctccggtcgc
     4981 tgctctcgag acgattccta tcgctttctc ttccacgctc tccattgagc actccccctc
     5041 agtaccaccc tgtcatcacc atcatcattg aatgtttcgg ggggtggttc cttcctgacc
     5101 caaccatggg atcgagcagc gatttttttt caccattccc cgcccctact cccgcacccg
     5161 gaaccagatc gcccggtcat gaatgatgtc gtcgtaatcg gcaccagagc ccgtggccgg
     5221 gttgaggtac gcccggtagc cgatgatgat tgggtcggta aggttgatac agtagctggg
     5281 gctttcgcac ggatggtccg aagtcgcggt cggcacgatg acaaagtact tgtcgtgctt
     5341 gtcctgcggc ggctggcgca gcaccttcac cgcgtacagg tagggcgcca tcgggtcccc
     5401 ggggatgtat tcctcggcac tcccggtaaa atcatggttg gtcatgctcg cgaccccgtt
     5461 ccagctccgg tcaccgcacc cgaacacgta ctgccacgga ttgtcctcgc acgaactggt
     5521 aacccagtcc ccatataccc cgaaattgga ataggtagcc agtccggtcg ccgcatggtt
     5581 gaccccgtag actaccacca tgtcgtcatt ccccagcgta aaattggcgg cctcgccgga
     5641 gagcgatgtc cgcaggtagg gcgtgtcgga agcttccccg gcaacgaact tgtggtagag
     5701 gggcgaactg ggatcagtct cctccagcac ctcccttgaa tcggcaaacc agcggatgga
     5761 atcataggat tcgtactgga catggggcgt ctgggcgagg atggcgttct ccagtcgctc
     5821 cagcccattg ttcaggtcgg gatagagctc gctctcgctc ttccatgtgc ggacccgttg
     5881 ctcgggcgtg gggaatgggt ccagtgtcag cgcctcttca ggggtaaccc gccataccct
     5941 agcatagtaa tcgtcacgga ggtaatggtc cccctcggcc ttgctgacga agttggccgt
     6001 gcgcatcagg atggtaaacg agtcagcgag cggggtctcg ggaaagacac cgagattcag
     6061 cagcccggaa ggaatgacat aggggttgat catggatttg ggataaccgg cctcctgggc
     6121 cagggccgcc acgcgctcat aca
//
LOCUS       DAOOCO010000115         6122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12083_length_6122_cov_4.757046, whole genome shotgun sequence.
ACCESSION   DAOOCO010000115 DAOOCO010000000
VERSION     DAOOCO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_6122_cov_4.757046"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PK154_07150"
     CDS             <1..600
                     /locus_tag="PK154_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPW10874.1"
                     /translation="SSRMGSPKAVLPLGGKTVLEHIVQTYLLAGITDIRVVTGHDREQ
                     VRSVLEKIPAGEVNNPRHHEGMYSSVRCGVASCGPEISGYFIHPVDVPLITPRTIRSL
                     CASADPAGDRIACPVYEGRRGHPPFIGASFGRAILEEDRPQGLKGFLYEYSDAIVPVI
                     VDDPGILLNMNTPMDYQEVLRRMENTASDRRGRGNEKTT"
     gene            584..2950
                     /locus_tag="PK154_07155"
     CDS             584..2950
                     /locus_tag="PK154_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPW10875.1"
                     /translation="MKKLHDAHGATGIPDEKGEGHFHTKSDLPVPVGSDLFRADAYGK
                     VTGRERYAADEYPAGLVYAGVVRSPHPHARILAIDTTGALACPGVIAVLTGGDIRGTN
                     RTGLPEMDQPVLADTKVRFTGDPVVLVVAEDRELLRQAMAAVKVTYEPLPAVFDPDPA
                     LAKGAPVLHENRKEGNLLFSGVIAKGDADFAFSECAAVREGVFSLPRQEHAYLETETG
                     VAKVDGDGVLEMIVSTQSPFRDSREIAAALGIPPEKVRVIAPYLGGAFGGKDGITVQG
                     HLGLAALHSGGRPVKIWNSREESFVSSTKRHPGRVSLKLGCTKDGTLHALSCRLIFDT
                     GAYAGLGGPVAVYGMGHAAGVYRVAHSRVEGMNVYTNNPPSGAFRGFGIPQVIAALEQ
                     VVDMLAESIGMDPLEFRLKNAIRVGDETGLGIRMSNSADIVTCLEKARDHPLWTGRDE
                     WIRQAPDYKRRGVGIATGVLGIGYGPVIPDIASAKLELLTDGRFRIYAGVADMGQGNN
                     SANIHIAGDILCQSPDRFELVQPDTFRCLNSSSSAASRTTYSYGNALILAAGVLKERI
                     LDKVAQLHPLFRRENLILVPGAVRHTPDGSDIALHTIAEAMQEADRVATATWTAPVNR
                     QQLAVAPDVKMYGFPHLAYTFTTHLTRVEIDEITGAVAVDAYLAWTDAGRIMNPHVYE
                     QQIQGGVIQGMGYALWEDFRVTEGAVVTKNLSTYILPTSMDVPDVLSVPVETYEESGP
                     FGMKGVGEIGIVAALPCIANAVAGAIGSRIYDAPLTPERILTALRRAA"
     gene            2947..3417
                     /locus_tag="PK154_07160"
     CDS             2947..3417
                     /locus_tag="PK154_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPW10876.1"
                     /translation="MKIRCIVNGKQTDLETAPDRRVVDLLREDLGLCGTKEACGSGEC
                     GACTILVDGESRLSCLMIAAQLEGKEIVTIEGFSKEHLHPIQEAFIVHGAVQCGFCTP
                     GMVLAAADLLKRNPAPDRHEIRRAISGNLCRCTGYQKIVDAIESLARAEEESDE"
     gene            3410..4246
                     /locus_tag="PK154_07165"
     CDS             3410..4246
                     /locus_tag="PK154_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPW10877.1"
                     /translation="MSEVALPASLEEVYAIRKREPGAAVLAGGTDLLVRMRADGKKPP
                     VLIGLERIPGLSGIAIQDGVVRIGPMVTHQNLLDSPVIRKHLAVMHEAAQVLGSPPVR
                     HMGTIGGNICTASPAGDLLPALYVLDAEVEISSPAKKRVVPIATFITGPGRTILTGQE
                     ILTGIRIPVPDPGYQGFYRKVGQRRALAISIVSMAALLMVSPDKTVRSARFAWGSVGP
                     VVMRFPDVESSFAGRTFGPALLADVGRQYSERIRPISDIRASASYRKMVAAGLPLKIL
                     EQ"
     gene            complement(4303..4554)
                     /locus_tag="PK154_07170"
     CDS             complement(4303..4554)
                     /locus_tag="PK154_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPW10878.1"
                     /translation="MDESQIEKTLREILARRVPNAPIDKLTLDAPLAALGIDSLAYSW
                     ILADTEDTFGFVMMGSDVMKLKTLKHTIEYVQTQIEKKG"
     gene            complement(4585..>6122)
                     /locus_tag="PK154_07175"
     CDS             complement(4585..>6122)
                     /locus_tag="PK154_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW10879.1"
                     /translation="TYRELDLLSDAIAKSIIAMGIEKGTHAALWAPNIQEWPAILLGC
                     AKAGLPLLLVNTSFRSYDLEYCLNLVDVGILFLASGNGQPGEYISALYEACPGLEDSQ
                     PGKISSQKLPHLRTAVQIDDGNHRGILPWTAFLGQGEQVARTDLQARESSVRPEDRFI
                     FLFTSGTTGWPKCITWTHERLIGTTFTLADGFGLAPGDVVCIPPPFFHLLGCGILLSA
                     LSSSCTVAPLERFNPQEMLRTIETFRVTHIFGVPSMFVSALNEYNRTRYNTASLRGGV
                     VAGAVSPPAVVRAVMDILGAREFGICYGLSEVLATITRPSDPMECRTGSIGRPLRDTD
                     IRIVHPLDGTDVRPGETGEILVRSPWMISGYYDKPDTIVAATDDDGFLHTGDLGFVDG
                     DGYYHIAGRSRDLIIRGGENISPSEIEEFLLTHPAVMDAQVVGIKSEYYGEEPVAFVR
                     TKPGQAVTALGLKQFCRRSIAIEKVPSTFFFVDRYPLTASGKVQKFRLREMAAELLSE
                     QKR"
BASE COUNT         1267 a         1744 c         1862 g         1249 t
ORIGIN      
        1 tcgtcccgga tgggctctcc aaaagccgtg ctcccccttg gaggaaagac ggttctggaa
       61 catatcgttc aaacctatct cctcgccgga attacggata tccgggtagt gaccgggcat
      121 gatcgcgaac aggtccgatc cgttcttgag aagatcccgg ccggggaggt caataacccc
      181 cggcaccacg aggggatgta ctcgtccgtc cgatgcgggg ttgcctcgtg cggcccggag
      241 atctccgggt atttcatcca tccggttgac gtcccgctca tcacgccccg gaccatcagg
      301 tctctttgcg catccgcaga ccctgccggt gaccgtatcg catgcccggt gtatgagggg
      361 cggcgaggcc atcccccgtt catcggggca tcgtttggaa gagcgatcct tgaagaagac
      421 cgcccacaag gcctcaaagg atttctttat gagtattcgg atgcaatcgt tccggtaatc
      481 gttgatgatc cgggaatcct tttgaacatg aatacaccca tggattacca ggaagtgctc
      541 aggagaatgg aaaatactgc cagcgatcgc cggggaaggg gcaatgaaaa aactacatga
      601 tgcccatggg gccacaggca taccggatga aaaaggggaa gggcattttc atacgaaaag
      661 tgacctgccg gtcccggtcg gatcagatct ctttcgggcc gatgcttatg gaaaggtgac
      721 cggcagggag cgttatgcag cagatgaata ccctgccggg ctggtgtatg ccggggttgt
      781 caggtctccg cacccgcatg cccgaatcct cgctatcgat acgacagggg cgcttgcctg
      841 ccccggtgtc atcgctgtcc tgacaggcgg ggatatccgg gggacgaacc ggaccggcct
      901 tccggagatg gaccaacccg tccttgccga tacaaaggtc cggtttaccg gggatccggt
      961 ggtgctggtc gttgctgagg acagagagct cctcaggcag gcaatggctg cggtcaaggt
     1021 gacgtatgaa cccctccctg cagtattcga cccggacccg gctctcgcaa agggagcccc
     1081 cgtcctgcat gaaaaccgta aggaaggaaa tctgctcttc tccggcgtga tcgcaaaggg
     1141 agatgcggac tttgcatttt ccgaatgtgc agctgtccgg gagggtgtct tctccctccc
     1201 gcggcaggag catgcgtatc ttgagaccga gaccggcgtg gcaaaggttg acggggacgg
     1261 ggtgctcgag atgatcgtat cgacccagag cccgttccgg gattcccggg agattgccgc
     1321 ggctctcggt attccacccg aaaaggtccg cgtaatcgcc ccgtatctcg gcggtgcgtt
     1381 tggcggcaag gacggcatca cggtgcaggg gcaccttggc cttgcggccc tccactccgg
     1441 gggaaggccg gtcaagatat ggaattcgcg cgaagagagt ttcgtcagct cgacaaaacg
     1501 ccatcccggc cgggtatcct tgaagctggg atgcacaaag gacggcaccc tgcacgccct
     1561 ttcctgccgg cttatctttg ataccggcgc ctatgccggc cttggcggcc cggtcgccgt
     1621 atacggcatg gggcatgcgg caggggtata ccgtgtcgct catagccgtg tggaagggat
     1681 gaacgtgtac accaataatc ccccatcggg agcattccgg gggttcggga ttccccaggt
     1741 gatcgcagcc cttgaacagg tcgtggatat gcttgcggag agtatcggga tggatccgct
     1801 ggagttcagg ctgaagaatg cgatacgtgt gggcgatgag accggtcttg gaatccggat
     1861 gtccaactcc gccgatattg tcacctgcct tgaaaaggcc agggatcacc cgctctggac
     1921 aggccgggac gagtggatac ggcaggcccc cgattacaaa aggagagggg tcggcattgc
     1981 gacaggagtt ctcggcatcg ggtacggtcc ggtcattccc gatattgcca gcgcaaaact
     2041 ggaacttctc accgacggcc ggttcaggat ctatgcgggg gtcgcggata tgggccaggg
     2101 caacaattcg gcaaacatcc atatcgccgg ggatatcctc tgccagtccc cggaccggtt
     2161 cgaactggtc caaccggaca cgttccggtg cctgaattca tcgtcctcgg ccgcaagcag
     2221 gacgacctac tcgtacggga atgcccttat cctcgcagca ggcgtgctga aagaacggat
     2281 cctggataag gtcgcgcagc tgcatcccct cttccggaga gagaacctta tccttgtgcc
     2341 cggggcggtc cggcatactc cggatgggag cgatatcgca ctccatacaa tcgcagaagc
     2401 aatgcaggag gcggacaggg ttgcaacagc aacctggacc gcgccggtca accggcaaca
     2461 gcttgccgtt gcacccgacg ttaaaatgta tggtttcccc caccttgctt atacgttcac
     2521 cacgcacctg accagggtag agatcgacga gatcaccggg gcggtggccg tcgatgcata
     2581 cctcgcctgg acggatgccg ggcggatcat gaatcctcat gtatacgagc agcagatcca
     2641 gggaggagtc atccagggca tggggtatgc actgtgggag gatttccggg taacggaagg
     2701 ggctgttgtg acgaagaacc tctcgaccta catcctgccc acctcaatgg atgtccctga
     2761 cgtcctgtcc gttcccgtgg agacttatga ggagagcggg ccttttggca tgaaaggggt
     2821 tggggagatc gggatcgtcg cggcactccc ctgcatcgcg aatgctgttg ccggggctat
     2881 cggatccagg atctacgatg ccccgcttac gccggagcgg atccttaccg ctctccggag
     2941 ggcagcatga agatccgatg catcgtcaac ggaaagcaaa ccgatcttga aacggctccc
     3001 gaccgaaggg tcgtcgacct cctccgggag gatctgggcc tctgcgggac aaaggaagcc
     3061 tgcgggtcag gggaatgcgg cgcctgcacc attctcgtcg atggggagag ccgtctctcc
     3121 tgcctgatga ttgccgccca gcttgaagga aaggagatcg tgacaataga aggtttttca
     3181 aaagagcacc tgcacccgat ccaggaagcg ttcatcgtgc atggggcagt ccagtgcggg
     3241 ttctgcacgc cgggcatggt cctcgcggca gcagatcttc tgaaaagaaa tcccgcgccg
     3301 gaccggcatg agatcaggag agcgataagc gggaacctct gtcgatgcac cggataccag
     3361 aagattgtcg atgccatcga atccctggcc cgggcagagg aggaatccga tgagtgaagt
     3421 ggccctcccc gcatcgctcg aagaggtgta tgcgatccgg aagcgcgaac ccggtgctgc
     3481 ggtgcttgcc ggagggacgg atctcctcgt gcggatgcgg gctgacggaa aaaagccgcc
     3541 ggtcctgatc ggtcttgagc ggatacccgg gctttcaggg atagcgatac aggatggggt
     3601 cgtccgaatc gggccgatgg tcactcacca gaacttgctc gacagcccgg taatcaggaa
     3661 acacctcgcg gttatgcacg aggcggcaca ggttctcgga tcgccgccgg tacgccatat
     3721 gggtacgatc gggggaaaca tctgcaccgc ctcgcctgcc ggcgatcttc ttcctgccct
     3781 gtatgtgctg gacgctgaag tggagatatc ttctcctgcc aaaaagcggg ttgtccctat
     3841 tgcaacgttt atcaccggcc cggggaggac aatcctgacc ggccaggaga tccttaccgg
     3901 gatcaggatc cctgttcccg atccggggta tcagggattt taccggaagg tgggccagcg
     3961 gagagccctc gcaatatcca tcgtcagcat ggcagccctg ctcatggtct ccccggataa
     4021 gaccgttcgt tctgcccggt ttgcctgggg gagcgtggga ccggttgtca tgcggtttcc
     4081 agacgttgaa tcatcctttg ccggcaggac gtttggcccg gcgcttcttg cagatgtcgg
     4141 gaggcaatat tccgaacgga tccggccaat cagcgatata cgggcttccg cctcgtaccg
     4201 gaagatggtg gcggctggcc tgcccctgaa gatccttgag cagtgaaaaa aggcggctac
     4261 gggcaccctc cggcatcgcc tcaacgaggc tgccgtccgg gtttatcctt tcttctcgat
     4321 ctgtgtttga acgtactcga tcgtatgttt gagtgttttg agtttcatga catccgagcc
     4381 catcatgacg aatccgaaag tatcttccgt atcggcaagg atccacgagt acgcgagcga
     4441 atcgattccc agcgctgcaa ggggtgcgtc cagggtgagt ttatcgatcg gggcgttcgg
     4501 tacgcggcgg gcaagtatct cccgcagggt cttctcaatc tgagattcat ccatatttct
     4561 aaaactctct cgtagaatat atattcatct tttttgttcg gagagtaatt cggctgccat
     4621 ctccctgagc cggaacttct gcaccttccc gcttgccgtc agggggtacc ggtcgacaaa
     4681 gaagaaagta gaagggactt tttcgatggc aatactgcgc cggcagaact gtttgagccc
     4741 gagggccgtt accgcctgcc cgggttttgt gcggacgaat gccaccggct cctcaccgta
     4801 atattcgctc ttgatcccga ccacctgtgc atccatgacg gcagggtggg tgaggaggaa
     4861 ctcctcgatc tccgagggag atatattctc cccgccgcgg atgatcagat ccctgctccg
     4921 gccggcgata tggtagtagc cgtccccgtc cacaaagccg aggtcgccgg tatggaggaa
     4981 cccgtcgtcg tcggttgcag caacgatcgt atcgggctta tcgtaatagc cggatatcat
     5041 ccaggggctc ctgacaagga tctccccggt ctccccgggc ctgacgtccg taccgtccag
     5101 gggatggacg atccgtatat cggtgtctct cagcggtctc ccgatgctgc ctgtcctgca
     5161 ctccatagga tcggacggac gggtgatggt cgctagaacc tcgctgagtc cataacagat
     5221 cccgaactcc cgcgctccca atatatccat gaccgccctg acgacggccg ggggggagac
     5281 agcccccgcg accacacccc ccctgagcga cgccgtgttg tagcgggttc ggttgtactc
     5341 gtttaaggcg ctcacgaaca tggaaggcac cccgaaaata tgcgtgacac gaaaagtttc
     5401 gatggtcctc agcatctcct gcgggttgaa ccgttcgaga ggcgctaccg tgcagctgct
     5461 ggaaagtgcg ctcagcaata tcccacaccc gagtaaatga aagaacgggg gcggaatgca
     5521 gacgacatcg cccggtgcga gaccaaagcc gtccgcaaga gtgaacgttg ttccgattaa
     5581 ccgttcatgt gtccaggtta tgcacttcgg ccagccggtt gtccccgagg taaaaaggaa
     5641 gatgaagcga tcttcgggcc ttacggaact ctcccgtgcc tgaagatctg ttcgggcaac
     5701 ctgctctccc tgcccgagga atgccgtcca gggcaggatg ccccgatggt tcccatcgtc
     5761 aatctgaacc gcggtcctga ggtgcggcag tttttgcgag gagatcttcc ccggttgaga
     5821 atcctcaagt ccggggcagg cttcgtacag cgccgagata tattcgcccg gctgcccgtt
     5881 tccggaagca aggaacagaa tacctacatc caccagatta aggcagtact ccagatcgta
     5941 ggatctgaaa ctggtattca caaggaggag aggaaggccg gcttttgcac atccgagaag
     6001 gatggccggc cattcctgga tgttcggtgc ccagagggca gcatgcgtgc ccttttcgat
     6061 acccatggca atgatgcttt ttgctatcgc atccgagagc agatccagtt cacggtaggt
     6121 ga
//
LOCUS       DAOOCO010000116         5998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12439_length_5998_cov_5.078580, whole genome shotgun sequence.
ACCESSION   DAOOCO010000116 DAOOCO010000000
VERSION     DAOOCO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12439_length_5998_cov_5.078580"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..389
                     /locus_tag="PK154_07180"
     tRNA            317..389
                     /locus_tag="PK154_07180"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:351..353,aa:His,seq:gtg)
     gene            940..1374
                     /gene="msrB"
                     /locus_tag="PK154_07185"
     CDS             940..1374
                     /gene="msrB"
                     /locus_tag="PK154_07185"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPW10880.1"
                     /translation="MVRIYNVSTGATSEVEKIRKSEEEWRSVLSNDAFRIARRGETEP
                     AFTGLYHDFHGRGIYQCVCCGTDLFTSETKFDSGTGWPSFSAPVSDTNILTRPDTSYG
                     MVRIEVLCARCEAHLGHVFDDGPHPTHKRFCMNSASLRFIPG"
     gene            1569..2573
                     /locus_tag="PK154_07190"
     CDS             1569..2573
                     /locus_tag="PK154_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPW10881.1"
                     /translation="MDHRCSISILCAALSCMAAQKNRFRDSFFTLFSEVFGERIATIL
                     ILLYLSSGILRIGTIAAGIGQYLSFFGIDDILLIELLIIALFCGINLIGITLSGITEN
                     LLTALKVIPLVVIALLLLPSVELQNLTPLVPITATGLVKTVLLVYWPFTGFEISTIPV
                     DEMRDSRMVPYALAIVMLVVSAEYLLLNIALIGSVGSAVLAASPAPIATAAALILSRS
                     GSTVAVIGIIAMLSALNAYLVGGSRLLHSTAVALHVSSLTSLNPRGAPMAPLLLIALL
                     SAGILSPSNSFEALAVLSVLTILVPYWCISCGSGIFKALDHYCRNCQLGRITSPFISF
                     "
     gene            complement(2676..3038)
                     /locus_tag="PK154_07195"
     CDS             complement(2676..3038)
                     /locus_tag="PK154_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10882.1"
                     /translation="MQVFLSNGQIFSVNLSPLSFTVSNGFALLPGSMSAKPWKISRGC
                     GDYAAIDSATGLWFVPWTIVMFGARMGNNYREKRILFQMPAISWTTGTHEKTMIAAAI
                     RIFSLIYTPSIMRRINVV"
     gene            3296..4024
                     /locus_tag="PK154_07200"
     CDS             3296..4024
                     /locus_tag="PK154_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW10883.1"
                     /translation="MAGHASAEVRNGTIGEVIGLSGTAPGFYVVYLFMTGPGVPQYGS
                     RMDSSVSPVVTGDPDSFTQVVVDGDRWNYSWQTGRVSGGLAPGLYTVYASTAPVAADA
                     LSGIPYSETDVFLSKPVTTGTFHIQSVPPGAQVEVNGKYSGNAPLDLPDLVPGTYLIN
                     ISLQGYVPENGVYDLSAGETKQISVELKPALPSATDSPLPLTTIPETMAGTAQSPTRA
                     PLSSLIVIGSLVIGLGMMIGLIQE"
     gene            complement(4280..5077)
                     /locus_tag="PK154_07205"
     CDS             complement(4280..5077)
                     /locus_tag="PK154_07205"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HPW10884.1"
                     /translation="MRVILRTKPRKKPFIPIEAEAIIPQNFLRKNDIHVYEGNKERNL
                     EELFYITTEGPAVSPDQVEIVLSGETGIIKRVGEYMNAGRIIVEGDIGMHCGNFMSAG
                     EIIIKGNAGAWLGREMRGGIIHCEGNAGHYCAAGYRGEKRGVRGGLVEVMGNAGDFTA
                     EYLSGGEIRVQGDCGDLPGVEMRGGTLIIGGSCHRPCGNMTGGTCMIFGRVYDMLPTF
                     RNTGSEEREYGGQRCQMMLYKGDAANRGKGTLLVKKTEQVGLSPKKE"
     gene            complement(5074..>5998)
                     /locus_tag="PK154_07210"
     CDS             complement(5074..>5998)
                     /locus_tag="PK154_07210"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HPW10885.1"
                     /translation="RQSLYLTHVQFHSYGGSTWKDISSKSEDIARAVNNKPEVAIDMG
                     QIMFGRTTTMTADGPMEFKLYMLHHNKWSNHDVELETGSGIIPVYYSRKSLVNCVMWA
                     IGLELALLVKDPWRCLLSTDNPNGAPFVKYPEIIALLMSRRFRENEMALLDPRTAHYV
                     PLPAIDRELDWYDIAVMTRAGQARALGITGIGKGNLAPGAEADIAIYPLKPESLDPSA
                     EYQQVMAGFGKTRYTIKRGRVVARDGDVVVEGANSTIWTKATVPPEYDLSVDPEFVKK
                     FEQYYTVRMSNYPVQDVYLPRGVCIPTEASQ"
BASE COUNT         1374 a         1590 c         1485 g         1549 t
ORIGIN      
        1 gacatgcccc atgacgcaga caataggcgt cctgatcttc gggttctccg ccataatccg
       61 atcctccatt attgttcaag ccagaaacac gggaaaaaac accacgcctt catgcccgga
      121 tgatatgtcc ggcacgcatc aacgtcaggg aagtgtgttt cgggccacct gatatgtaat
      181 ggacttctga caatattaat tgtcaccccg cggacgggga tggcttcccg taaaagtaat
      241 acctgttgtc agcactgtcg acgcattggc aaggatacat taatcaaaga acagtgcaaa
      301 aattaaaggg caatttgcca gggtggggta gttggttatc ctaggggact gtggatcccc
      361 cgacccgggt tcgagtctcg gccctggcct ccctttcttt tcccgttctt acacctgcac
      421 tacaggtctg tcatcggtga gtcacaccgg ggacacttta gggcatagca gggcagagtt
      481 acagatcctt tccgttcata tccgcaattc ggacagcggc atgtcacctg atcgatcatg
      541 ggtccagccc ctctcctccc tgccggacca tccgcttccc tccctctcat caaagcacca
      601 cataagcagg gggactcttc catcatcaat tttcttaggg taaggatagc tgagctggtc
      661 tgatgcggaa gtgctcatcg ttgagggaga taaaggatga gcagaattcc gggatacaat
      721 gaggaggatt aatatctcgg aagaatgtca catccattgt agtcaaatag aggctgaaat
      781 actccgttcc agtaaatgaa tgtaagatcc catggccgct gttcggtcga gatgagagtg
      841 tgataatgct ggagatccct tccgttgggg tatactctac cctttttatg tcgatgtaat
      901 gaatacacca ctctttatga aagaagcggg agtgaactga tggtccgcat ttacaatgtc
      961 agcacgggtg ccacgagcga agtggaaaag atcagaaaga gcgaggaaga atggcgaagc
     1021 gtcctttcaa acgatgcatt ccggattgcg cggagaggtg agacagaacc agcttttacc
     1081 ggattatatc atgattttca tggcaggggg atataccagt gtgtctgttg tggcacggat
     1141 ctcttcacct ccgaaaccaa gtttgattcg ggaaccggct ggccgagttt ctctgccccg
     1201 gtaagtgaca cgaacattct gacccggcct gataccagct acgggatggt gagaatcgag
     1261 gtactctgcg cccgttgtga agcccatctt ggccatgttt ttgacgatgg acctcacccg
     1321 acacacaaac gattttgcat gaactcggct tcattacggt ttattcccgg ctgatccagg
     1381 aaaattcagg aatgtagatt caaaaatttt ttggaatcga cggggtaggt gatgaaagag
     1441 gagggctatc tcaaaacact tggtctcccg gaactggtaa gccttggtgt tggtgggacg
     1501 ataggatctg gtattttcgt ggttcctggt cttgcagccg gcattctggg tccttactcc
     1561 cttcttgcgt ggatcatcgt tgcagtatca gcatcctgtg tgctgctctc tcttgcatgg
     1621 ctgctcagaa aaacagattc agggattcct tcttcaccct tttttcagag gtctttggtg
     1681 agcggatcgc aacaatcctt atccttctgt acctctcctc aggaattctg aggatcggga
     1741 cgattgctgc cggaatcggg cagtatcttt ccttcttcgg gatagatgat atccttctta
     1801 tcgaattgct catcatcgca ctattctgtg gaatcaacct gataggcatc accctttcag
     1861 gaataactga aaacctcctg accgctctca aggtaatacc gcttgtcgta atcgccctcc
     1921 tccttcttcc ctccgttgaa ttgcaaaatc tcaccccatt ggttccgatc accgctacgg
     1981 gtcttgttaa aacggtcctt ctagtgtact ggccctttac gggatttgaa ataagcacca
     2041 tccctgtgga tgagatgaga gattcacgga tggtacctta cgcattggct attgtcatgc
     2101 tggtcgtctc ggcagagtat ctcctcctca atattgccct catcggaagt gtggggagcg
     2161 cggtgctcgc tgcctctcct gctccgattg ccaccgcggc agccctgata ttgtcccgat
     2221 caggatcaac tgttgccgtt atcggcatta tcgcgatgct ttcagccctc aatgcttatc
     2281 tggtcggagg gtcacgactc ctccatagca ctgccgtagc gctgcatgtg agttcgctta
     2341 cctccctgaa tccccgcggg gctccaatgg ccccattact ccttattgcc cttctctccg
     2401 cgggcatcct ctctccttca aacagttttg aggcgcttgc cgtcctttcc gttcttacta
     2461 tacttgttcc ctattggtgc atatcgtgtg gttccgggat tttcaaagcg cttgatcact
     2521 attgcaggaa ttgccagttg gggaggatta cttctccttt catttctttt taaaagggga
     2581 tgactgaaag aaaaatgaaa attggaaaga aacggatgag tgatatctgg caacaaagaa
     2641 gaataataac agaaccagaa gcatctgcct attttttata cgacattgat cctcctcata
     2701 atggaaggtg tgtaaatgag agagaatatc ctgatggccg cagcaatcat tgttttttca
     2761 tgtgtacccg ttgtccagct gatagccggc atctgaaaca aaatcctttt ttcacggtaa
     2821 ttatttccca ttctcgctcc aaacatcaca atcgtccatg gcacgaacca gagaccagtt
     2881 gctgagtcaa tggcggcata atccccgcat ccgcgggata ttttccatgg cttggcgctc
     2941 atggaaccgg gtaataaagc aaaaccgttg gatacggtaa aagagagagg ggagagattg
     3001 accgagaaaa tttgtccatt tgataaaaaa acctgcatca gagatcaatg tgccgttttc
     3061 agggatgatc tcgacaggtg tgcattcctg ctgataggaa aacagtcggg agctcatcca
     3121 tactcccagc ggagcacggg ggatcaaccc tccgggaagt tcaaagccca tctctttgaa
     3181 tgaaaaaagg ggaaaatttc aaaaagcaac gatgctgata gagctcactg gtgattatgt
     3241 ggatcttggt tctctgaagc agcaaaccgt tcttctctgt ctcttactca tcctcatggc
     3301 cggacatgca tccgcagagg tgaggaatgg taccatagga gaggtcatcg gactatccgg
     3361 caccgctcct ggattctatg tggtgtacct cttcatgacg gggcctggag tgccgcagta
     3421 tggatcaaga atggacagca gtgtatcccc ggttgttacc ggagatccgg actcgttcac
     3481 tcaggtggtg gtggatggag accggtggaa ttattcatgg cagaccgggc gggtctctgg
     3541 ggggctcgct ccagggcttt acacagtata tgcctcaaca gcacccgtgg cagcagatgc
     3601 cctgtcagga atcccttatt cggaaacgga tgtttttctt tcaaagcccg tcacaacagg
     3661 gacgttccac atacaatctg tccctcccgg ggcacaggtg gaagtcaatg ggaaatattc
     3721 ggggaatgcc ccgctcgatc ttcccgatct cgttcctgga acgtacctga tcaatatctc
     3781 tttgcagggg tatgttcctg aaaacggggt atacgacctt tccgcgggag agacaaaaca
     3841 gatatcggtg gaactgaaac ctgcgcttcc ctccgcgaca gattcccccc taccccttac
     3901 aacgattcca gaaaccatgg ccggtactgc ccaatccccg acccgggccc ccctttccag
     3961 tcttatcgtg attggatcac ttgtcatcgg gcttgggatg atgattggtc tcatccagga
     4021 gtaatgtttt cctgaagaaa tcgaacgatg ggggtatagc ataaccttat tacgcagttg
     4081 aactgatgca tcctatgagg tgagaggtaa tgtgcaccgt tggtggacct gtcgatatgg
     4141 aaattccccc tgagcgagtg aaggggaaga attaccgatg caaagagtgt ggagagagat
     4201 tcaagggggt agggaagcgg ccgatgtgcc cctcctgcca gtctgaagat gttgttgaag
     4261 aataagtaag tgcagcggat tattcctttt ttgggctcaa tccaacctgt tcagtttttt
     4321 tgaccaggag agtccccttc cctcggttgg cggcatcccc cttgtaaagc atcatctggc
     4381 agcgctggcc tccatattcc ctctcttcac taccagtatt cctgaaggtc gggagcatat
     4441 catacacacg cccaaagatc atgcaggttc ctcccgtcat gttcccacac ggcctgtggc
     4501 agctgccccc gattataagg gttcccccac gcatctcgac cccggggaga tcgccgcagt
     4561 cgccctggac cctgatttca cctccactca gatactctgc cgtgaaatct cctgcattac
     4621 ccatcacttc gacaaggccg cctcgcacac cccttttctc tccgcggtat cccgcggcac
     4681 agtagtggcc tgcattccct tcacaatgga tgattcctcc ccgcatctcc cggccgagcc
     4741 aggcaccggc attccctttg atgattatct cgcctgcact catgaaattc ccgcagtgca
     4801 tcccgatgtc tccctcaacg atgatgcgtc ctgcgttcat gtactctccg acccgtttga
     4861 taatcccggt ctcccctgaa aggactatct caacctggtc cggagagacg gcaggtccct
     4921 cagtggtgat ataaaacagt tcttccaggt tccgttcctt gttcccttcg tacacatgga
     4981 tgtcattttt acggaggaaa ttctgcggaa tgatagcctc cgcctcgata gggatgaagg
     5041 gttttttcct cggtttcgtc ctgagaatta ccctcattgt gatgcctcag tcggaataca
     5101 gaccccccgg ggaagatata cgtcctggac cgggtagttg ctcatcctga ctgtgtaata
     5161 ctgctcaaac tttttcacaa attcgggatc gactgagaga tcatattcgg gaggaacggt
     5221 tgctttcgtc cagatcgtgc tgtttgcccc ctcaacgaca acgtccccat cccttgccac
     5281 cacccgacct ctcttgatgg tataccgggt ctttccgaat ccggccatca cctgctggta
     5341 ttcggcagag ggatccaaag actccggttt taatggataa atggcaatat cagcctcggc
     5401 accgggggca agattcccct tgccgatgcc ggttatgcca agagctcgtg cctggccggc
     5461 cctcgtcatg acagcaatgt cgtaccagtc gagttccctg tcaatagcag gcaggggtac
     5521 gtaatgggcg gttctcggat caagaagcgc catctcgttc tccctgaacc gcctgctcat
     5581 aaggagtgca atgatctccg gatatttgac aaacggtgca ccgttgggat tatcggtcga
     5641 gagtaggcac ctccatggat ctttcaccag aagagccagc tcaagcccga tcgcccacat
     5701 gacacagttc acaaggctct tgcgggaata gtagacgggg atgatccccg atccggtctc
     5761 gagctccaca tcatggttgc tccacttgtt gtggtgcagc atgtacagct tgaactccat
     5821 cggcccgtct gcagtcatgg tcgtcgtcct cccaaacatg atctggccca tgtcgattgc
     5881 gacctctggt ttgttgttca ctgcccgggc gatatcctct gatttcgagc tgatatcctt
     5941 ccacgtcgaa ccgccatacg agtggaattg aacgtgggtc aggtagagac tctggcgc
//
LOCUS       DAOOCO010000117         5992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12449_length_5992_cov_4.276739, whole genome shotgun sequence.
ACCESSION   DAOOCO010000117 DAOOCO010000000
VERSION     DAOOCO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12449_length_5992_cov_4.276739"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..690
                     /locus_tag="PK154_07215"
     CDS             154..690
                     /locus_tag="PK154_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10886.1"
                     /translation="MEQAGSLRSDDEVRVLLKSYLSSMDIEVVKEVEKDGNWGIWTQF
                     GNFPVLIDHLKDTHYCVVAFQITLQDEHAIIHLNNFYERNDAQFIYELTRAFSSPLTA
                     FSRIVERGRVIGYTVSKNIYPYHPEFTLRTLDIALQAVVSTGAIGIAFLKWMARVLDV
                     SHERGIEIKDEDPTKLFE"
     gene            complement(754..981)
                     /locus_tag="PK154_07220"
     CDS             complement(754..981)
                     /locus_tag="PK154_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10887.1"
                     /translation="MRQGAKRITMCDRICYRKKGDLYECFRISGDGDEELLGKGSRDD
                     IISWLSSHGLGFVQKEVLLQDFELLCEEKEE"
     gene            1043..1726
                     /locus_tag="PK154_07225"
     CDS             1043..1726
                     /locus_tag="PK154_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPW10888.1"
                     /translation="MMSDSFPVRFISWEESASLARTLAARIKDDRSKQPDLVIAIGRG
                     GYVPARVVCDELLTTRLTSIRLEHWGPGARRKERAVVRYPLSCSVADRDLLVIDDVSD
                     TGDTLSVAISYLQELQPSRIRTGVLHHKASSRLTPDYYAELVKEWNWIIYPWALHEDI
                     CGFLEIELTSHPVSLPDLHSILLKRYNLQITTDEMVSAVEDLIRLGKITKNGELLFAC
                     ENGINTSES"
     gene            1808..2512
                     /gene="rnc"
                     /locus_tag="PK154_07230"
     CDS             1808..2512
                     /gene="rnc"
                     /locus_tag="PK154_07230"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPW10889.1"
                     /translation="MKSEPNRAAEIRNLLMRPEIGRRRISPASLRVYDAALTHRSYAK
                     EQSGYDNVVSDNERLEFLGDRVLNLVIADFLFSTFDEPEGSLSLRMEWTKNRNLASVI
                     SSAAPQLPGLIRLGKNQALTPRIVAGAFEAFIGALYLDAGLDTVKKMVVMLFSENIQT
                     FSTNTNYKKSLQEYLQKQNLPVPVYELERCEGLPHSPLFSYVVKSGERMLGKGVGKNK
                     SEATQNAARNALSGIS"
     gene            2638..3279
                     /locus_tag="PK154_07235"
     CDS             2638..3279
                     /locus_tag="PK154_07235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW10890.1"
                     /translation="MNILYVYAHPEPRSFNGALKDTAMVTLGKLGHTVKVSNLYAVRW
                     KAILDSQDFRMRQDPYQFNPALEQIHGIRTGSLSPDILEEIERVAWADLIICQFPLWW
                     SSMPAILKGWFDRVFVQGFVVNIEKGQVYERGLLKGKKAMVVVTTGATQEMYSPNGVH
                     GDIMHHLSLITHNIFEFVGMTPLPSFIVFGAAGMTRDEGSLQLDRYKTVLEKL"
     gene            complement(3290..3676)
                     /locus_tag="PK154_07240"
     CDS             complement(3290..3676)
                     /locus_tag="PK154_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10891.1"
                     /translation="MLNIEGKSSEAKIGKILILVSVMLGVLIILLLVALIMIIRPTGT
                     LTGFGLMMMIPLWILISLLALKVLGVILGILALYYTEKNEFSTAGILAAVSSVLPPID
                     LIMLIGGIFCLISKEANELRAPPPLL"
     gene            complement(4248..4874)
                     /locus_tag="PK154_07245"
     CDS             complement(4248..4874)
                     /locus_tag="PK154_07245"
                     /inference="COORDINATES: protein motif:HMM:NF002177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HPW10892.1"
                     /translation="MLPTVPGNGPTGYSVADYAAIRDICEHLKASSTKGELAEKIARE
                     LEKFSVLALQVICGRLHHEVERLPSPYREAVRPFFIQQLFGAHHQIMLMFRNGSFWKM
                     RETLTDQVLISEYFLMVQKACFSRETQSDYVPGFNSPYQGLFYFLIAAFQMFVLEGPG
                     HPVGMPFPGGFRVEDRNGVYFCPVRDKEKEVPYSICNFCPAKQTDTLK"
     gene            complement(5028..5450)
                     /locus_tag="PK154_07250"
     CDS             complement(5028..5450)
                     /locus_tag="PK154_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HPW10893.1"
                     /translation="MLETVNKFEKGVYYALMIMLGVVIFFGVIELIVIIYSALVTDLS
                     YHLSNHEILEIFGYFLLILIGIELLETIKAYLIKNEIHVEIIILVAIIAVARKIILLD
                     PFVEGAEALNSEGMIALGVVIIALAASYFLVKKSGTGT"
     gene            5563..>5992
                     /locus_tag="PK154_07255"
     CDS             5563..>5992
                     /locus_tag="PK154_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW10894.1"
                     /translation="MNKSVLIGITFIMAFSLVASGASQQGTGEAAGYFSVASVPGEAQ
                     VVMDSIYRGNTPVVVPVPLTGTPSHTLTVSKPGYLPWVRTYETGPGAGQTFAVNAVLE
                     PSAERGTLIVTTSPAGALVTIDGTGGQQAPWTYRDIPSGSH"
BASE COUNT         1560 a         1446 c         1492 g         1494 t
ORIGIN      
        1 tataaacctc ctatgagccc cgatttcggg atacgggcat acattatgaa aattacgaat
       61 tgattttcat tggaaaaagg gatgaatagc agattggctt gatccgatcc gcggatcaat
      121 accctcataa gggtgcgacg aatatctcct cccatggaac aggccggttc tcttcgcagc
      181 gatgatgagg tgagagttct tctcaagagc tacctctcaa gtatggatat tgaagtggtc
      241 aaggaggttg aaaaagacgg gaactgggga atatggaccc agttcggtaa tttcccggtc
      301 ctgatcgacc atttgaagga tacgcattac tgcgtggttg ccttccagat aactttgcag
      361 gatgagcatg cgataatcca cctgaacaat ttttatgaaa ggaatgacgc ccagtttatc
      421 tacgagctga cgcgggcttt ctcatcgccc ctcacggcat tttccagaat tgttgaacgg
      481 ggcagggtca tcggttacac ggtctcgaaa aacatctatc cctaccatcc cgaattcacc
      541 ctccgtaccc tggatattgc ccttcaagcg gtggtgagta cgggggcgat tggtattgcc
      601 ttcctgaaat ggatggcaag agtcctggat gtcagccacg aaaggggaat agagataaaa
      661 gacgaggacc cgacaaagtt gttcgaatag accgcctccg ggaccgccgg ctcagagcac
      721 atttcttcag gatgccaggt agaatgcaga tcctcactcc tctttttcct cgcagagtaa
      781 ctcaaagtcc tgaaggagca cctccttttg gacaaaccca agtccgtgag aagacagcca
      841 ggagatgata tcgtcccgag atccttttcc aagaagttcc tcgtctccgt ccccggatat
      901 tcggaagcat tcatagagat cgcccttttt ccgataacag atccggtcac acatggtaat
      961 tctttttgca ccttgacgca ccagaggata aaactggcga tcagggaaaa aaatttagag
     1021 atggggagga aggtaagggg taatgatgtc cgattcattc ccggtccggt tcatttcgtg
     1081 ggaggagtcc gccagcctcg cccggacgct tgcagctcgg ataaaagacg acaggagcaa
     1141 acagcctgac ctcgtgatag ccataggcag gggtggctat gtccctgcac gggtggtgtg
     1201 cgatgaactg ctcactacca gacttaccag catcagactc gaacactggg ggccaggtgc
     1261 gcgccggaaa gagagagccg ttgtcaggta tcccctgtcc tgttcagttg cagatcgtga
     1321 cctccttgtc atcgatgatg tcagcgatac cggggatacc ttgtccgttg caatcagtta
     1381 cctgcaggaa ctccagccat cccggatccg gactggtgtt ctccatcaca aggcatcttc
     1441 ccggctcact cccgactatt atgcagaact cgtcaaggaa tggaattgga tcatctatcc
     1501 ctgggcactt catgaagata tctgcggatt cctggagatt gagctgacaa gccacccggt
     1561 ttcattacca gatcttcaca gcatactgct gaagagatat aacctgcaaa tcacgactga
     1621 tgagatggta tctgccgttg aagatcttat ccgtttggga aaaattacaa agaacggaga
     1681 attgctcttt gcatgtgaaa atggaataaa tacctcagaa tcctgaagat ctctctcttt
     1741 tatcatgcct gggttgccgt tacttcccgg aattattaat acggagatgc aatgaatttc
     1801 aggaaatatg aagagtgaac cgaaccgggc agcagagatc cggaacctcc tcatgcgtcc
     1861 tgaaatcggg cggagaagga tctcacctgc gtcattgagg gtatatgatg ctgcactcac
     1921 ccaccggtca tatgcaaagg aacaaagtgg ttacgacaac gtggtttctg ataacgaacg
     1981 actcgaattt ctcggtgaca gggtactcaa tctggtgatt gctgacttcc tcttctctac
     2041 atttgatgaa cctgaaggtt cgctctcgct ccgcatggaa tggacaaaga accgtaatct
     2101 tgcctcggtc atctcttcag cggctcctca gttacccgga cttatccggc ttgggaagaa
     2161 ccaagccctt accccccgta tcgttgcagg agcatttgag gcgttcatcg gcgcgctcta
     2221 tctggacgca ggcctcgata cggttaaaaa aatggtcgtt atgctctttt cggagaatat
     2281 ccagacattt tcgaccaata ccaactataa aaagagtctg caggaatact tacaaaagca
     2341 gaatcttccc gttcctgttt atgagcttga acggtgcgaa ggtctgcccc attcccctct
     2401 tttttcgtat gtggtcaaat caggagagag gatgcttggg aaaggtgtgg gaaagaacaa
     2461 gtcagaggca acccagaacg ctgcccgcaa cgctctcagc ggcatctcgt gataatcggt
     2521 tctctttctc atgcgtgctc tccttctgga cagatggacg tccctccttt gtattttata
     2581 caaccccctc tgaaaaccta tttcttgata ccggtcaaga ctgtgtctgg tgatggtatg
     2641 aatattcttt acgtgtatgc tcatcccgaa ccacgctcct ttaacggtgc actcaaggat
     2701 actgcaatgg ttacccttgg gaaactgggg catacggtaa aagtatcaaa tctgtatgcg
     2761 gtcagatgga aagcgatcct ggattcacag gatttccgga tgcggcagga cccgtatcag
     2821 ttcaatccgg cattggagca gatccatgga atccggacag ggagcctctc acccgatatc
     2881 cttgaagaga tcgaacgggt agcatgggcg gatctcatta tctgccagtt tcccctctgg
     2941 tggtcatcga tgcccgcgat cctgaaggga tggtttgaca gagtgtttgt ccagggcttt
     3001 gtggtaaata ttgaaaaagg ccaggtatat gaaaggggat tgctgaaggg gaagaaagca
     3061 atggtagttg ttaccaccgg agcgacccag gagatgtaca gccccaatgg tgttcatggt
     3121 gacattatgc accatctctc cctcatcacg cacaatatct tcgaatttgt cggcatgact
     3181 cctctgccat cgtttattgt gttcggagca gcgggtatga caagggatga aggcagcctt
     3241 cagctggaca ggtacaagac ggtgctggaa aaattataaa gagaaagtat cagagcagcg
     3301 gcggtggtgc ccgtaactca ttggcctctt tgctgatgag acaaaatatg cctccaatga
     3361 gcatgataag gtcaatggga ggaagtacag agctgaccgc agcaaggatc cctgcagttg
     3421 agaattcatt tttttctgtg taatacaatg cgaggattcc aagaattact cccagcactt
     3481 tgagcgcaag gagggagatg agaatccaga gcggaatcat catcatgaga ccgaatcctg
     3541 tcagcgttcc tgtcgggcgg atgatcatga tcagagcaac gagaagcagg atgatcaaca
     3601 cgccaagcat gacggaaaca aggatgagga tcttcccgat ttttgcttca gaacttttcc
     3661 cctcgatatt gagcatgctg ttgtacctta tctcttctat aaatccctga atccggatta
     3721 aactatcctg ctcataggag tctggaattc ccctccaatt cccggttctc ctggaaaccg
     3781 gggcaacagt ttcatgatac ccgtacctct tccaattttt aaccgtcaat ccgaaaagat
     3841 caagcgaatg acgcgaaaaa tatttcttgg ttaaccggga gccacatata cccggagcca
     3901 gagcaggttg tcctttcttt ccaagatcaa ccgaaggact ctgataatag caggtatctc
     3961 cccagaaagt ctgttttttt gggcctgtgg ccgcttaaag agattttcat tgaatggaga
     4021 tcaggaagga tagtccgctg gggtggacct gaagaggaaa gtaattcacc ccgacagaaa
     4081 aaggaagtct tcttcaagtg gtcctgtagt cgtgttcaca agtgttatta tctcctggtt
     4141 cggagagcat cgttatcggt cctgttttcg gatgaagaga ggtgaggccg gggaaggaaa
     4201 tgtcacgaga ggaatgtact gagatcacct ttccatgaat aaagggtcta tttgagagta
     4261 tccgtctgct ttgcaggaca aaaattgcag atggaataag gtacttcttt ttctttgtca
     4321 cggacgggac agaaatacac cccattacga tcctcaacac ggaatcctcc cgggaaaggc
     4381 attccaacag gatggcccgg cccttcgagt acaaacatct ggaacgcagc gatcaggaag
     4441 taaaaaaggc cttggtaggg agaattgaag ccggggacat agtctgattg tgtctcgcgt
     4501 gaaaagcagg ccttctggac catcaggaaa tactctgata tcaatacctg gtcggtaagg
     4561 gtttcgcgca ttttccaaaa cgatccattg cggaacatca gcataatctg gtggtgagca
     4621 ccaaacaact gttgaataaa gaacgggcgg actgcctcac ggtaggggga tggaagacgt
     4681 tcgacctcat gatgcagtct gccacagatc acctgcaggg ctaagaccga aaatttttcc
     4741 agttcccggg caattttctc ggcaagttcc ccttttgtgg aagatgcctt gaggtgttca
     4801 cagatatccc ggattgcggc gtaatcagcg acagaatatc cagtggggcc attgccgggt
     4861 acggtgggaa gcattcccat tctgtctttt tctgtaggtg tgagcagtaa aaatatcttc
     4921 tcctgcacta tcctgctgtt ttgtacatcg gatacatggg aaccgactga tccacccggg
     4981 tctcccctca gtttagaaaa aatggaagca ggtgcctttc agacagttca ggttcctgta
     5041 ccactcttct tgacaaggaa gtaggaagca gccagggcaa ttatcactac cccgagtgct
     5101 atcattcctt cactattcaa cgcttccgct ccctcaacaa agggatcaag aagtattatc
     5161 ttccgggcga cggcaatgat tgcgacaagg atgatgatct caacatgaat ctcattcttg
     5221 atgaggtatg ccttgatcgt ctcaagcagc tcgataccta tcagaatcag gagaaaataa
     5281 ccaaaaatct cgagtatctc atgattggaa aggtggtaag agagatcagt taccagtgca
     5341 gagtatatga taactatcag ctcaatgacc ccgaaaaaga tgaccactcc aagcatgatc
     5401 atgagtgcgt aatacactcc cttttcgaat ttgtttacgg tctcaagcat ctgtttttcc
     5461 tctctcctct cctttggtct catggaggtt atcagtgctg ctggatatac ctgccgggaa
     5521 acatatatca agggttaaat aattggatgc atttccctta tcatgaacaa gtcggttctt
     5581 atcgggatca ctttcatcat ggcattttcc cttgtcgcat cgggagccag ccagcagggg
     5641 accggtgaag ctgccggata cttctccgtt gcatcagtcc cgggagaggc acaggttgtc
     5701 atggacagta tctatcgggg aaatacccct gttgttgtcc cggttcctct gactggaacg
     5761 ccctcccata ccctcaccgt atccaaaccc ggatatctcc cctgggttcg gacctatgaa
     5821 accggtccag gtgcgggaca gacgttcgcc gtcaatgccg tgttagaacc aagcgcagag
     5881 aggggtacgc ttattgtcac cacctctccg gctggtgccc ttgtgactat agatggaacg
     5941 ggagggcaac aagccccgtg gacctaccgc gatatcccct cgggaagcca tg
//
LOCUS       DAOOCO010000118         5987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12462_length_5987_cov_4.198921, whole genome shotgun sequence.
ACCESSION   DAOOCO010000118 DAOOCO010000000
VERSION     DAOOCO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12462_length_5987_cov_4.198921"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..148
                     /locus_tag="PK154_07260"
     CDS             11..148
                     /locus_tag="PK154_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPW10895.1"
                     /translation="MLWISIVATMILFYPVRKTAAWLLVPYILWVSFATLLTYTIYSL
                     N"
     gene            complement(209..2128)
                     /locus_tag="PK154_07265"
     CDS             complement(209..2128)
                     /locus_tag="PK154_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HPW10896.1"
                     /translation="MPVSEYDTVYRHSIDDPETFWGHAAEGISWEKKWNKVLSNSSGP
                     YYRWFEGAILNTCYNCLDLHVERGRGDQTALIYDSPVTSVIRKFSYRTLRDLVAQCAG
                     GLIREGVRKGDRVVIYMPMIPETVIAMLACARIGAIHSVVFGGFAARELAARIRDAQP
                     RMIISASCGIEITRVIPYKPLLDEAIQLAETGPLRCVIYQRHQLLASLVEGRDISWDQ
                     LMDNDQPATCVPCKATDPLYILYTSGTTGVPKGVVRDNGGHLVALNWSMSNIYGMKPG
                     EIFWAASDVGWVVGHSYIVYAPLAYGCTTILYEGKSVGTPDPGAFWRVIAEHGVHVLF
                     TAPTAFRAIKREDPDGMHIKKYDLSKFRMLFLAGERCDPDTLLWAEKRLNVPVVDHWW
                     QTETGWAIGANTAGLGLLPIKPGSCTKPVPGYDVQVLDEHGEELPPSQIGNIVIRLPL
                     PPGCLTTLWNNERDFSTTYLAEYPGYYLTGDAGFIDNEGYLWIMGRIDDIINTAGHRL
                     STGAMEEVLASHQDVAECAVTGVADEVKGEIPIGFVVLKTGVIRDPSGIEQELIRLVR
                     ERIGPIASFKIAIVVRRLPKTRSGKILRGTMKKIADGTEYRIPATIDDPTILDEIKES
                     LARIGYPKKTRYSKN"
     gene            2288..2845
                     /locus_tag="PK154_07270"
     CDS             2288..2845
                     /locus_tag="PK154_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPW10897.1"
                     /translation="MEILTVAIIAIALAVDCFAVSLGAGTVITERRILSAAVMGLFFG
                     GFQMGMALAGWAAGSWIASMIGFIDHWIAFCILAVIGVKMMYEGFIGEETPERNYLNL
                     TTLIILAIATSIDSLGVGLSFALLSTGIVLPALIIGITSFIFSFLGVLLGSRLAAHFG
                     RPVEIAGGIVLVGIGIRILAGHLAS"
     gene            2924..3451
                     /locus_tag="PK154_07275"
     CDS             2924..3451
                     /locus_tag="PK154_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10898.1"
                     /translation="MMNSRDMFLIAVLLSTGTILRFVLVMAGELVTPNVMVAFYSVII
                     MVFLRSFGGAIGIGLLSGIITALISQSLVNPAFLLSEPLGAAVCLITFLSLQYHKRVA
                     SFVAAFAATVASGVAYTALALSMSSARILAAFSDPTVFLFRMTIVITVTALVNACMAS
                     LLFHAIRTYQDSIRP"
     gene            3505..3873
                     /locus_tag="PK154_07280"
     CDS             3505..3873
                     /locus_tag="PK154_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10899.1"
                     /translation="MEDIYKKIYLKLNRMGVFDVRQYAVIEKPPFTPLCIDRIADDCY
                     ALSQNRVEEGSVISDPDVEIRVDHKKKVAEPTSFQDRSVRKIVYPKHGTVNLQMKNEL
                     ASFLDRWLDDLLSQGFIRHQ"
     gene            complement(4935..>5987)
                     /locus_tag="PK154_07285"
     CDS             complement(4935..>5987)
                     /locus_tag="PK154_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPW10900.1"
                     /translation="GIPHWIALLLGAAIVTAYTVIGGLYAVAWTTLVQGLILIVGVII
                     MAPFVINAAGGFSAVNEYLGSLDPVSVLPWSSGQGAVFTPEYIVSFFVLLTIGLACAP
                     HVINNVLAAKDANYFKWTPLVAFIIYGVVMFLLKFSGFAGVVLVKEGIVTLPAVKNAS
                     DFIFVYGVQHAFSSLVLWGVFAVIVLAAVMSTTDRLMLTIGTYFSWDIYKKILRPNAS
                     DDKVLKVSRISVFSAAMVTLLMALNPPDMLVWLIWAGIGIMFATFAVPLLAGLYWRRA
                     TRQGAIAAMALGLGSALVFGGISYFKIASLPVHFSLYGFFIALGSMIVVSLLTRPHSE
                     EVLNKTMTGWYIRKVE"
BASE COUNT         1481 a         1484 c         1393 g         1629 t
ORIGIN      
        1 tgtcattatc ctgctctgga tctcgattgt ggcaacaatg attcttttct atcctgtccg
       61 gaaaactgct gcctggcttc tcgttcccta tatcttatgg gtttcatttg ctacacttct
      121 aacctacact atttacagcc tcaattgaag gtttcggaaa aataatccct ttttaccagg
      181 atccggggat tctaaaaagt tgttgttctt agttctttga gtatctggtt tttttggggt
      241 atcctatcct tgcaaggctc tcttttattt catcgagaat ggtaggatca tcaatggttg
      301 ccggtatccg gtattcggtt ccatcggcaa tttttttcat ggtcccccga agtatctttc
      361 ctgatcgtgt ttttgggagc cttcggacca cgatggctat cttgaacgat gctatgggcc
      421 caatccgttc ccgtacgagg cggatcagtt cctgttcgat accggaggga tcacgaatca
      481 cgcctgtttt gagaaccaca aatccgattg ggatctcccc cttcacttca tcggcaactc
      541 ccgtaactgc acattcggct acatcctgat gggatgcaag cacctcttcc attgcccctg
      601 ttgagagccg atgccctgca gtattgatga tgtcgtcaat acggcccata atccagagat
      661 aaccctcgtt atcgatgaaa cctgcatccc ctgtaagata ataaccgggg tattcagcca
      721 aataagtggt agagaagtca cgctcattgt tccagagcgt ggtaaggcag ccgggcggaa
      781 gagggagccg tatcacgata tttccgatct gggaaggggg aagttcctca ccatgttcat
      841 cgagtacctg tacgtcatac cccggtactg gtttggtaca tgaacccggc tttatcggca
      901 gcagacccag acctgccgta ttagccccta tcgcccaacc tgtttctgtc tgccaccaat
      961 gatcaaccac tggcacattc agcctctttt ctgcccataa gagcgtatcg ggatcgcatc
     1021 gctctccggc aagaaagagc atccggaact ttgacagatc gtatttttta atgtgcatac
     1081 cgtccgggtc ctccctctta attgcacgaa aagccgtcgg ggcggtgaag agcacgtgta
     1141 ctccatgctc tgcaatcacc ctccagaatg ccccggggtc aggggttccc accgattttc
     1201 cttcgtagag gatggttgta cagccatatg caaggggagc atagacaata tacgaatgcc
     1261 ctactaccca ccctacatct gacgccgccc agaagatctc tccaggtttc atcccatata
     1321 tatttgacat actccagttg agtgcaacga gatgcccgcc gttatcccgc accaccccct
     1381 ttgggacacc tgtggtccct gaagtgtaga ggatgtagag gggatctgtc gctttacaag
     1441 gaacacatgt tgcaggctga tcattgtcca tcagctgatc ccatgaaatg tcccttcctt
     1501 caacaaggga tgcaaggagc tgatggcgct ggtaaatgac acatcgcaaa ggacctgttt
     1561 ctgccagctg tattgcttca tcaagaagag gtttatacgg aattacccgt gtgatctcaa
     1621 tgccgcagga tgctgaaatg atcattctgg gttgtgcatc tctgattcgt gcagccagct
     1681 cccgggcagc aaaaccgcca aataccacgg aatgaattgc acctatccgt gcacaagcca
     1741 gcatcgcgat gactgtttcc gggatcatgg gcatatagat aactacccga tcccccttcc
     1801 ttaccccttc cctgatcagt cctccggcac actgtgccac caaatccctc aaggtacgat
     1861 agctgaattt tctgatcaca gaggtgacag ggctgtcata aataagggct gtctgatcgc
     1921 ctcttccccg ctccacatgg agatcaaggc aattgtaaca ggtgttgaga atcgcacctt
     1981 caaaccagcg gtaatatggg ccgcttgagt ttgatagaac tttattccac tttttttccc
     2041 aagagatccc ttcggcagca tggccccaga aggtctccgg atcatcgatc gaatgccgat
     2101 atacagtatc atattcagaa acaggaatag aacttcaccc tgccaagtta tagcatggca
     2161 agtattctct tcagacgata aaaaactttt tcctcttaca tttaagtcat ttcacgctat
     2221 actgccgtgg aattttcgaa ccaatacttg cacaggctta tctggtgata tgtcacacgt
     2281 aatcccaatg gaaatcctca cggtcgcaat cattgcaata gctcttgctg ttgactgttt
     2341 tgccgtttca ctgggtgctg ggaccgtgat aacggaaaga aggatattat ccgctgcggt
     2401 gatgggattg ttcttcgggg ggttccagat gggaatggca ctcgccggat gggcggcagg
     2461 aagctggatt gcatcgatga tcggtttcat cgatcattgg atagcgttct gcatccttgc
     2521 tgttatcggg gtaaaaatga tgtatgaagg cttcattggt gaagaaaccc ctgaacggaa
     2581 ttatctgaac ctgacaaccc tgatcatcct tgctatcgcg acaagcatcg attcactcgg
     2641 cgttggttta tctttcgccc ttctctcgac cggcattgtt ctcccggctc tcattatcgg
     2701 cattaccagt ttcatattct cattccttgg cgtattatta gggtcccggc tggccgcaca
     2761 ttttggccgt ccagtagaga ttgccggggg gatcgtactt gtgggaatcg gtatccgaat
     2821 tctcgcaggg catctcgctt cttgaatgcc acattattcc aaggaaggtc tttgttcgac
     2881 aataaggtac ttatcctggg aagttacagg gcattcctga tccatgatga attcacggga
     2941 tatgttcctg attgcagttc tgcttagtac aggcacgatc cttcgatttg tgctcgtcat
     3001 ggccggagag ctggtgactc cgaatgtaat ggttgcattc tatagtgtaa tcataatggt
     3061 ctttttgcgt tcatttggtg gggcgatagg gatcggactt ctcagtggta tcattaccgc
     3121 gctcatcagc cagtctctgg ttaatcctgc ttttttgtta tccgagccat tgggtgcggc
     3181 ggtttgcctg ataacctttc tttcccttca gtatcacaag agagtggcat ccttcgttgc
     3241 cgcattcgct gcaacagttg cgagtggggt tgcgtataca gctctggctc tgtcaatgag
     3301 cagcgcccgg attcttgctg cattttccga tcccacggtc tttcttttca ggatgacgat
     3361 agtgatcacg gtaacagccc tggtaaatgc ctgcatggcc agcctgctct ttcatgctat
     3421 caggacttac caggacagta tcaggccgta aggggtaatg tttataggtt cgctctctga
     3481 attgcgtata aagaaggtat gcaagtggaa gatatctata aaaaaattta cctgaaactg
     3541 aacaggatgg gtgtctttga tgtccggcaa tacgctgtta tcgaaaagcc gccttttaca
     3601 ccactatgca ttgacaggat cgctgatgac tgctatgcac tgtctcagaa ccgggttgag
     3661 gagggttcag tgatatctga cccggatgtt gaaatccggg ttgaccataa aaagaaggtg
     3721 gctgaaccaa cctcattcca ggaccgatca gtccggaaaa tagtgtatcc caaacacggc
     3781 acggtcaatt tgcagatgaa aaacgaactt gcttcatttc tcgaccgttg gcttgatgat
     3841 ctcttatccc agggatttat ccgccaccaa tagaagggaa ataaagggac aactctcaaa
     3901 ctccagttgg gggatcccgg tatcacctcc gtcagagcct ctgagtatgg aatatgatcc
     3961 ggttaataat tcccgtatcg ttttttatcc ggaagaatac aaaagattta ataagcaatc
     4021 ctttcaattt tatatcaggt gtattagtgt ccaactaagt caccgaatga acgttcagtc
     4081 caatggttga cgtcctgcct ggattcaggc agggctgaga cctcctgagg atgccacctg
     4141 aaactttcaa gagaaatggg gtggatttct tttctgtgaa tcatgaaatc atgaggttta
     4201 tctctttcat acattcagat caagttcttt cattcattca gatcaagtat tagtctgctg
     4261 ccgtgcgtta agagcatagt tgtgttactg ttcagtaccc caaatgcacg agtctcatac
     4321 aaagcattca tttgagtctg tatgcaccaa ccattcgaga aaatgagggt gccccaggag
     4381 gtttgtgcgg atcacatggt cagccctctg gcactgtgtc tttctcatac tccggtcttc
     4441 cgccgttctg accggattat tcacagtaac agagcatcat gttctccaga tctctgtgcg
     4501 gcccagtgaa cgggaacgtc tttatcacct atccatcgtc atgccgttat gatggatgga
     4561 tccgttccgt gctgcatcgg atctccctga acatttgttc ggcccagaga tttgacctct
     4621 ttgtcgcggg aagatcttcg agccgtttgg atcttccggc tggccaaatc tttgcacgta
     4681 tccccagaat tcccctctga ggcatatccc gatatgcact aatgctataa atattttctg
     4741 ttgttctttg tttttatatc catcgaaaaa aagactttaa taaaagacgc gcaggatatg
     4801 cggtcgatag tatacttcag caggacttcc atcctggatc gccttcgctc atgtcaaata
     4861 atcgtgagta ttttcattta gccggattta acgaaggatt ggaggtaaaa aaggatattt
     4921 caatgcgaga agaatcattc cactttacgg atgtaccatc ctgtcatggt cttgtttaat
     4981 acctcctcgc tgtgaggcct tgtgagcaga ctcacgacaa tcatagaccc cagcgcgatg
     5041 aaaaagccat acagggagaa atggaccgga agtgaggcta ttttaaaata gctgatgccc
     5101 ccaaaaacaa gggcggatcc tagtccgagt gccattgctg cgatagctcc ctgcctggta
     5161 gctcgtcgcc agtaaagccc ggccaggagc gggactgcaa aggtagcaaa catgatgccg
     5221 atacctgccc atatgagcca taccagcatg tcgggtggat tcagggccat gaggagtgtt
     5281 accattgctg ccgaaaatac cgagatcctg ctcactttca acaccttatc atcagatgca
     5341 ttcggccgga gtatcttctt atagatatcc catgagaaat aggtaccaat agtaagcata
     5401 agccggtctg tggttgacat gaccgctgca aggacaatga ctgcaaaaac cccccagagt
     5461 accaaggacg agaatgcgtg ctgaactccg tacacaaaga tgaaatcaga ggcatttttc
     5521 actgccggca acgttactat cccttccttt accaggacga ctcctgcaaa acctgaaaat
     5581 ttcagaagga acatgacaac tccatagatg ataaaagcaa ccagaggtgt ccatttgaaa
     5641 tagttggcat cctttgccgc caggacattg ttgatcacat gtggtgcaca tgcgaggcct
     5701 atggtgagga gcacgaagaa tgatacaatg tattcaggtg tgaacaccgc gccttgcccg
     5761 cttgaccagg gaagaaccga gacaggatcg agagaaccga gatactcgtt cactgcagag
     5821 aaacctcctg cagcattgat gacaaagggt gccatgataa tcactccaac gatcaggatc
     5881 aatccctgta ccagcgttgt ccaggcaacg gcatacaatc ctcctatcac ggtatatgct
     5941 gtcacgattg ctgcaccaag caatagggcg atccagtgcg ggattcc
//
LOCUS       DAOOCO010000119         5976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12485_length_5976_cov_4.894612, whole genome shotgun sequence.
ACCESSION   DAOOCO010000119 DAOOCO010000000
VERSION     DAOOCO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12485_length_5976_cov_4.894612"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..886
                     /locus_tag="PK154_07290"
     CDS             194..886
                     /locus_tag="PK154_07290"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPW10901.1"
                     /translation="MEILPKGRLDAFADGVFAIVITLLVLDLAIPEVVEGVMPALLDQ
                     WPAFLAYVISFSFVGSLWITHSSITALMKQETPHSYRLTLLMLFFVSLIPFTTKLMAF
                     YLSHPPMIPDLASSDFLSWWHTVNALARSALSAPVTLYGLDLLAASVTMNAIIKSAIK
                     TPDMLITESASPELDALQRRQQKGVNIIIGTLIFGFILPVVAVFGYIIVSIVFFLTPT
                     ITVAKTTLGKDK"
     gene            complement(1166..1975)
                     /locus_tag="PK154_07295"
     CDS             complement(1166..1975)
                     /locus_tag="PK154_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW10902.1"
                     /translation="MKVLVIMGSPRKGNTYRAAERIREIIQEKVPVEWEYVMLKEAHL
                     EDCRGCYQCFERGEEYCMLKDEASLIEQKMKEADGVIFATPVYGFQVSYLMKLFIDRH
                     GYIFHRPRFFRQKALLLTSAGAVGRKDVLKYLTLVASIWGFDVAAKVGIRSHATFGPL
                     PPYVIQENEKKLRRAADDFLAALIRGTRSRPNLFDVLAFHIGRAPSDELGERALADHQ
                     YWTDQGWLEKGRRYYVDVPVNPLYHALGVLAEWFMRRQIRRDLEAVSGIST"
     gene            complement(2177..2629)
                     /gene="hgcB"
                     /locus_tag="PK154_07300"
     CDS             complement(2177..2629)
                     /gene="hgcB"
                     /locus_tag="PK154_07300"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HPW10903.1"
                     /translation="MFDSYRENTLAYYPERCINCRRCSQVCPHGVFAEGEETAELVHK
                     TRCMECGACALNCPVQAIEVQSGVGCAWAMISAAIRGRDMDSCTCGGDAGSCCAESTE
                     ESSCCAENHEASTCCSGNTDRSSCCDDGATGSSGCSGTGDTASCCGKK"
     gene            complement(2629..3723)
                     /gene="hgcA"
                     /locus_tag="PK154_07305"
     CDS             complement(2629..3723)
                     /gene="hgcA"
                     /locus_tag="PK154_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPW10904.1"
                     /translation="MSENRVTRSSSDTASCSCCSGVQQQDALPCSGEPSPNIIPAITT
                     TTSTLTFADRLDHFLARWGVNRMGHVVKPGLYQLGKPTPDSPVFVSANYTLSFDSLRS
                     ALSGIDAYLLVLDTRGINVWCAAGKGTFGTDELVKRIGNTGLVSVVRHRAVIVPQLGA
                     PGISAHQVRERSGFRVEYGPVRARDLPEYLKTRKATPAMRRVEFPFRERIVLSPVELV
                     HAALPTIVAAILLYFLAGPVASLAAVTTILAGTVLFPALLPFLPTKDFSTKGLILGIL
                     VALPFAAYAWTQFSLPSWARLLAGIMPLFLIPPVVSYLALNFTGCTPFTSRSGVKAEI
                     FTYVRVMVAMAACGIVLAVLFRALLFLWVA"
     gene            complement(3716..4012)
                     /locus_tag="PK154_07310"
     CDS             complement(3716..4012)
                     /locus_tag="PK154_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HPW10905.1"
                     /translation="MVKKKLHDTPAPSAVSSPDCGPGTCCTVEAVLSVDERGQMVLPK
                     EVRQKAGIATGDKLALISWERNGEVCCLALLKAGNLSGMVQGILNPLVQDLKNE"
     gene            4133..4468
                     /locus_tag="PK154_07315"
     CDS             4133..4468
                     /locus_tag="PK154_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10906.1"
                     /translation="MRPVQGYADQSPLRFGDRFQQRGLVHSHLLRHLAGIDLPRMIEI
                     DTVGGTRQDLLSQEGQDIEISGNPGDIIADGEDLMPLSLLRGMFPRVVALAKSFISAI
                     AGIPGSSEG"
     gene            4695..>5976
                     /locus_tag="PK154_07320"
     CDS             4695..>5976
                     /locus_tag="PK154_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPW10907.1"
                     /translation="MIQKVSAVVVILLILAVLSIPVMAAGGNGGSVGADGAGSGGAAG
                     SEEGAGSGGSGGNDNAGSGAAGDAGTGATSSTGQAGPQAQTIQEEQVRNQQETADQPG
                     IQITRQDQDQTQSRTGVDTEGTTSPDTIRQKDRDTFLQQVSQLEQNLSRDQDRDQAHV
                     DLALSTFAIAGSVTGGAGPELSRLAGEINQSLAAAYQAEQQMSTRSSFMRKLIGGDRD
                     AAGLLIQSADQNQQRIQEMEQLLATCSDCDPQVRVKLEEQVQTLSQEQNRLLTLGQQE
                     QSDRGLFGWLFWGSSTVLAGGGQATPLTDDEKYWMTYMREEEKLARDVYLSLGARWNL
                     PIFMNIAQSEQVHMDSVKTLLDRYGIPDPAAGKAQGAFTDPALQNLYNDLIAQGSASP
                     VEALKAGVIVEETDIADLNKALATTEKNDIRTVYN"
BASE COUNT         1391 a         1699 c         1668 g         1218 t
ORIGIN      
        1 gatgctctcc tcattccact ccattcagcc cctccatgtt cacggggtta ctgcagggga
       61 tgttgcttca gtggaaggcc gggaggatat caccggaaaa cggggtacgg aaaagcagga
      121 tcaggatcat cagtgcatca cccggatgaa agagcaatgg tataaataga acgatagaaa
      181 gaaagatgga ttgatggaga tcctgccgaa aggccggctg gacgcattcg ccgacggggt
      241 atttgcaatc gtcatcacac tccttgtgct cgatctcgcc atccccgaag tggtggaagg
      301 ggtcatgccg gctcttcttg atcaatggcc ggcatttctt gcctatgtca tcagtttctc
      361 ctttgtcggc agcctctgga tcacccactc atctattact gccctgatga aacaggagac
      421 tccccattcc taccggctca cgcttttgat gctcttcttt gtctcgctca tccccttcac
      481 aaccaaactc atggcattct acctctcgca tccacccatg atcccggatc tggcaagctc
      541 tgatttcctc tcctggtggc atacggtgaa cgcactggcg agatcggcac tgagcgctcc
      601 ggtgactctc tatggcctgg acctgcttgc tgcgtcggtt acgatgaatg cgatcataaa
      661 atccgcgatc aagactcctg acatgctcat caccgaatca gcaagccctg agctggatgc
      721 actgcagcgg aggcagcaga aaggagtcaa tatcatcatc gggacgctga tcttcggatt
      781 catactcccg gtcgttgccg tattcgggta cattatcgtc tcgatcgtgt tctttctcac
      841 ccccaccatc accgtggcaa aaacgaccct ggggaaagac aagtgagaag cgggaatatc
      901 gtttcactgg gtagttccgc caggaagtgc tcctccatcg ggccggttga gtggcatcac
      961 ctctccggtc actgatgcaa caaccaccat cagggaagat tggtgagaaa ggggatgacc
     1021 ggcgtgcttg tcaatttttg agaagtggct tgccgggtgg agagaatgaa tgaagtatct
     1081 ccacgcagag gagatcgaag tgggaggacg cgagtgctct ccaaggctgc aggaatcttg
     1141 tttgaacggt cacaggcagt accggtcagg tcgaaattcc gctcaccgcc tcgagatccc
     1201 tgcgaatctg acgacgcatg aaccactctg caagcacccc cagggcatgg tacaggggat
     1261 tgaccgggac atcgacataa tagcgcctcc ccttctcaag ccacccctgg tcagtccagt
     1321 actggtgatc ggcaagagcc cgttcgccca gctcatctga aggggctctc ccgatatgaa
     1381 atgcgagtac atcgaacagg ttcggtcggg aacgggttcc cctgatgaga gcggcaagga
     1441 agtcatcagc cgccctccgt agtttcttct cgttctcctg tatgacatac ggagggagcg
     1501 gtccgaacgt tgcatgggat cgtatcccca cctttgccgc aacatcaaaa ccccagatac
     1561 tggccacgag agtgagatac ttcagtacat ccttccttcc caccgccccg gccgaagtga
     1621 ggagtaaggc tttttgccgg aagaaccggg gacggtgaaa gatatagcca tgccggtcga
     1681 tgaacagctt catcaggtag gagacctgga acccgtagac cggggttgca aaaatcaccc
     1741 catccgcctc cttcatcttc tgctcgataa gggatgcttc atccttgagc atacaatact
     1801 cctcaccccg ttcaaaacac tgatagcatc cgcggcagtc ctcgagatgt gcctctttga
     1861 gcatcacata ttcccattca accggcacct tctcctggat gatctcgcgg atccgttcgg
     1921 cggcacgata cgtattcccc ttccgggggc tgcccatgat aacgagaacc ttcatggaca
     1981 tcacttggtc gtacccggta aaaaacctct gaaagagatg caggccccga aaggtaccga
     2041 tccccatcac cattctgttc tcatcccggt cgagaaagcc acatggtggg cattttcttc
     2101 accagggata ggctttcgaa aaaagcatga tgcaaggatt ccccggtcgt ggagagacga
     2161 tacctccctg agggtatcac ttttttccgc agcatgatgc ggtatcaccc gttccggaac
     2221 agcccgagga tcccgtggca ccatcatcac agcaggagga ccggtcggta ttccccgaac
     2281 aacacgtaga cgcctcatga ttctcagcac aacacgaaga ctcctcagta ctctcagcac
     2341 aacaggaccc tgcgtctcca ccacaagtgc aactgtccat atctcttccc ctgatcgctg
     2401 cactgatcat cgcccaggca cacccgacgc cgctctgcac ctcaatcgcc tggaccgggc
     2461 agttgagggc acaggctccg cattccatgc accgtgtctt gtggacgagc tctgccgtct
     2521 cttcgccttc agcaaagacc ccgtgggggc agacctggct gcaccgccgg cagttgatac
     2581 accgctccgg ataatatgca agagtattct cgcgatacga gtcgaacatc aggccaccca
     2641 gaggaacaag agggctcgga aaagaacggc aaggacgatc ccacacgcgg ccatggcaac
     2701 catcaccctg acataggtga atatctctgc tttgaccccg gaccgggaag taaagggagt
     2761 gcagccggta aagttgaggg ccaggtacga tacgaccgga gggatgagga acagtggcat
     2821 aatcccggcg agcagacgag cccatgaggg cagggagaat tgggtccaag catatgctgc
     2881 aaagggcaga gcaaccagta tcccgaggat cagccccttc gtgctgaagt cttttgtcgg
     2941 gaggaacggg agcagggccg ggaacaggac ggttcccgcc agaattgtgg tgaccgcagc
     3001 aagggaggct accggaccag cgaggaaata caggagaatc gcggcaacga tggtcggtaa
     3061 cgctgcatgc acaagttcta cgggagagag aacaatcctc tctctgaagg gaaactccac
     3121 ccggcgcatt gcaggggtcg ccttcctggt tttcaggtac tccggcagat cccgggcacg
     3181 gaccggtcca tactcaaccc ggaatccgga ccgctctctg acctggtggg cggagatgcc
     3241 cggggctccc agctgtggca cgatgacggc ccggtgccgg acgacggaca cgagcccggt
     3301 atttcctatc cttttgacga gctcatcggt gccaaacgtc cccttccccg ccgcacacca
     3361 gacattgatc cccctggtat ccaggacaag aaggtaggca tcaatcccgg agagggctga
     3421 acggagtgaa tcaaagctga gggtatagtt tgctgagacg aagaccggag agtctggtgt
     3481 cggcttcccg agttggtaga gccccggctt caccacgtga cccatccggt tcacgcccca
     3541 ccgggcaagg aaatggtcga gccggtcagc gaaggtgagg gtgcttgtcg tggtcgtaat
     3601 agccgggatt atgttcgggg acggctcacc actgcagggg agtgcgtcct gctgctgaac
     3661 accggaacaa caggagcaag aggcagtatc agaagaagac cgggtcactc tgttttcact
     3721 cattcttcag atcctgcacg agtggattga ggattccctg gaccatcccc gaaagattcc
     3781 cggctttcag caacgcgagg cagcagacct cgccgtttcg ttcccagctg atcagtgcca
     3841 gtttgtcacc cgtggcaatc ccggctttct gccgcacttc cttggggagc accatctgcc
     3901 cccgctcgtc aacactcagg accgcttcaa cggtacagca tgtaccaggt ccacaatcag
     3961 gggatgatac agcggacggt gccggggtgt cgtgaagctt ctttttcacc atacgttcat
     4021 tcctgatcat ccttgatctt caatatatta ttatttttca gaataatcag ataaatattc
     4081 ctatgatccc cttgtctccc agagatgacg ggattatgga accagaccac tcatgcgacc
     4141 tgtgcaagga tatgccgatc aatcgcctct ccgctttggg gataggttcc agcagcgtgg
     4201 tcttgtgcat tcccatcttc tccgccatct ggccggcatc gatcttccaa ggatgatcga
     4261 aatagatacg gtgggaggaa cccggcagga tcttctttcg caggagggtc aggatatcga
     4321 aatttccggg aaccccgggg atatcatcgc agacggggag gatctcatgc ccctatccct
     4381 cctccgcggc atgtttccta gggtagtagc tctggcaaaa tccttcatca gtgccatcgc
     4441 aggcatccct ggttcatcgg aaggatgagg tacctgacgg tgccaaacaa ccttccgaag
     4501 ctctgcatag gggatatcag ggattttcca ggagagcaga ttcctgagta ccatactggg
     4561 gagccatggt ctggcgtccg gttataccgt tgtctgtaag agaggcggta tcgcccattt
     4621 tttgaaaaga gggatcatat ttttctcttc ccaccctcca aaggaagatg ctgaaactga
     4681 gcatgtgatg gaaaatgatt caaaaggtat ccgcagttgt tgtgatcctc ctcattcttg
     4741 ccgtactgag catcccggtc atggctgccg gggggaacgg aggatcggtt ggagcagacg
     4801 gagctggttc gggaggagca gcaggatcag aagagggagc aggttcaggg ggatcaggtg
     4861 gaaacgataa tgctggcagt ggtgcagcag gggatgccgg taccggtgca acgtcctcta
     4921 ccggccaggc tggtccccag gcacaaacaa tccaggaaga acaggtgcgg aaccagcagg
     4981 agacggctga ccagcccggg atccagatca cccggcagga ccaggaccag acgcagagca
     5041 ggaccggggt cgataccgaa ggaaccacca gtccggatac catccgccag aaagaccggg
     5101 atacgttcct ccagcaggtc agccagctgg agcagaacct ctcccgtgac caggaccggg
     5161 accaggcgca cgtagatctt gccctctcca cattcgccat tgccggcagt gtgaccgggg
     5221 gtgccgggcc tgaactttcc cgtctggccg gagagataaa ccagtcgctt gccgctgcct
     5281 accaggcaga gcagcagatg agcacccgga gttcgttcat gcggaagctc attggcggag
     5341 acagggacgc ggcggggctg ctgatccagt ctgccgacca gaaccagcag cgaatccagg
     5401 agatggagca gctgcttgcc acctgctcag actgcgatcc gcaggtccgg gtgaagcttg
     5461 aagaacaggt gcagaccctc tcgcaggaac agaaccggct cctcaccctg gggcagcagg
     5521 aacagagcga ccggggattg tttggctggt tgttttgggg ttcttcgacg gtgctggccg
     5581 gaggagggca ggctacccca ttgaccgacg atgagaagta ctggatgacc tatatgcggg
     5641 aagaggagaa actcgcccgg gatgtctatc tctcgcttgg ggccaggtgg aaccttccca
     5701 tattcatgaa catcgcccag agcgagcagg tgcatatgga ctccgtgaaa accctcctcg
     5761 accggtatgg tataccggat ccagcggcag gcaaggcaca gggagcgttc acggatcctg
     5821 ctctccagaa cctctacaat gacctgatcg cccagggaag tgcttctcct gtcgaggcac
     5881 tcaaggcagg agtcattgtc gaggagaccg atattgccga cctgaacaag gctcttgcca
     5941 caacagagaa gaatgatatc aggaccgtgt acaaca
//
LOCUS       DAOOCO010000120         5943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12595_length_5943_cov_4.686990, whole genome shotgun sequence.
ACCESSION   DAOOCO010000120 DAOOCO010000000
VERSION     DAOOCO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_5943_cov_4.686990"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(997..1671)
                     /locus_tag="PK154_07325"
     CDS             complement(997..1671)
                     /locus_tag="PK154_07325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW10908.1"
                     /translation="MALQEGDFITLSYTGSIAGSVFDTTDEEVAKEAGIYTPEGLYGP
                     VTIRVGSHHVILGLDEALVGKEVGFEGEVDVGPDKAFGPHDPKKVEAFNKNAFKDKPR
                     LGMHLSVEEKGEGTVVDVIGNRVLLDFNHPFAGKELHYWYRIESQVTSVEEKVNGLIR
                     LYAGRDMETAFADGILTITLPPGINYDRRWVLWRSRVIGESLEHIPEINEITLVETFK
                     RKEEAE"
     gene            complement(1890..2606)
                     /locus_tag="PK154_07330"
     CDS             complement(1890..2606)
                     /locus_tag="PK154_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10909.1"
                     /translation="MKPVLICLFLIGFSLAGSVSASGNASDYDWYGLLIEANPYAGCP
                     SCVFAYDNDGLLDPNPYAGCPVCVFAYDDDGLLDTNPFAGCPTCVFAYDDDGLPDPNP
                     YAGCPTCSFIFDTGIESDLNSPDSCPTCGTTQSSLKTGYSSLSISSNNAPGSTLYSFA
                     NGTPIPADYVCPIHGIYCPDDPRPIEDRPGYADIVSYEKPAMVSRTLPETTAEFMELF
                     TTAVSIPKSGRMDGILYRSR"
     gene            2722..3249
                     /gene="cyaB"
                     /locus_tag="PK154_07335"
     CDS             2722..3249
                     /gene="cyaB"
                     /locus_tag="PK154_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPW10910.1"
                     /translation="MLEVEIKARVPDLAPIRERLLNCHEGVPERVHERDIYYNSPHRD
                     FRQTDEALRLRYAGGSCILTYKGEKRKEFRLKAREELNCVVESGEMIELILSRLGFFP
                     AAEVEKWREYYSYRGATVSLDEVKGLGSFVEIEAPGSPGYDHPEEFIREIAEELGVEG
                     EPILSSYLELLQSAP"
     gene            complement(3385..4026)
                     /locus_tag="PK154_07340"
     CDS             complement(3385..4026)
                     /locus_tag="PK154_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW10911.1"
                     /translation="MMDVLLLADLHGEYGKLDPFLDLNPDAVFIAGDLTNMGPAESVT
                     DVLDRIDVPCFAVPGNCDPRETIDVLEQSDCVCLHGAQINLGKISICGIGGSNPTPFS
                     TLFELTDKQIDDLLHKVLQKRELAMHNVLISHAPPYGLLDQANGNHVGSQSVRRHLTS
                     FDLVCCAHIHEERGIAIEEGVKVVNPGMAALGDGAMIHFGNEPKEIEIELITV"
     gene            complement(4023..5141)
                     /locus_tag="PK154_07345"
     CDS             complement(4023..5141)
                     /locus_tag="PK154_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HPW10912.1"
                     /translation="MKVHWRFTSGTRNTYAALYAACEREGILLSPVSAPAPDITCYSL
                     SSLNATSLLTEIEAGDCITIAGGPYATACYREVAEKADYVVVGEGERTLPELLFAIEK
                     GTADTVPGVATRHGFRPADSMVRLDAWPPFSQMKGYVELSRGCPFSCAYCQTPRIFGH
                     AMRHRSIDEVARYASRYRDARFVTPNALAYGSDGRRPCLAKVERLLKRLRNNIYLGTF
                     PSEVRPEFVSEEALSLITRYCANTSLQFGAQSGSDSVLEHLKRGHSVDDVIHAVELCR
                     DAGLKPVVDVIVGFPFETDEDQRATADLIRWISTYGTVHAHLFLPLPGTPLEKTSPVP
                     LIPELSHLLGSLALKGKVTGSWRDPRISFCGSCSNDRA"
     gene            complement(5186..5611)
                     /locus_tag="PK154_07350"
     CDS             complement(5186..5611)
                     /locus_tag="PK154_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10913.1"
                     /translation="MQNQVLFFILLSAMALLCAGCTESIPGIPPGSQPDSMTPGPTVT
                     MPPGQSVTVQINEKDPITATIKVIFAGGEGQIATRDILVRLTRSDGEVIIEHLTAEMQ
                     DEVDIQGTKESDRIEVYVTLNTGKTYKIIDQLVPYRTRG"
     gene            complement(5664..>5943)
                     /locus_tag="PK154_07355"
     CDS             complement(5664..>5943)
                     /locus_tag="PK154_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HPW10914.1"
                     /translation="EGESITGKVLAFPHGKGSTVGSYVLYALKRNKKAPAGIINTRAD
                     PIIAVGAIMGKIPMVDQPEEDIMQLRNGTCITVDGDTGTIEYDQDGIP"
BASE COUNT         1336 a         1585 c         1656 g         1366 t
ORIGIN      
        1 gaagaaaaat gccttcatca ccccggcgaa aggggaggcc gaggtgacca ggctttttca
       61 aggtagcgcg agttgcagaa ctcgcggagg gttttgtcct gcaccataaa acccttctgg
      121 aaggctccca atgagaggat cccgctgtgc tccaggtggg gcgaatgttc tatgcagaac
      181 agaacttcat tgtgcgattc ccataaaaga ggtttcggtt tgttccccag acgttcggtg
      241 acgtaagatg cagctctggg tttggcggga tcgcccggcc ggagtgaagg gcactgactc
      301 agcagggatt ttcttcattt tttccggaag gaactacgta tcagtgcgac agaatcccaa
      361 aaaatagctc taaagtccat gttctaccac tcaccctgaa aaatctccta ttattgaatc
      421 gtgctatcct ttcgtacacc gggtcttacc tgccggatcc aggtgctccc tcttttttag
      481 aggctgctca aaacacgggt cacctccatt tcattggcag gatggattac ctcctcctca
      541 tcttccggaa tcctgttcat cgctcacgta gctgacggga atctcactct gggaggatat
      601 cttgcaacga tggcatgaag ggagaaggaa aaaagtaact agttcagatt gatcctactt
      661 tttccgttca gaaccgacaa tcacaggatt ctctttcaaa ggggtgccgt tcatccggtt
      721 ccaccactta ttgtaatcct cctgccaata tttctgggga tttcttggtt cgtttgcccg
      781 catacgatta ccatagtact atcgttctct gacatgatag catagctgat ggcctccatt
      841 tttccagaga acgattgagg ggatccggca tcctggaaag tgaatggctg ttcgctggtg
      901 gatgtgccgg ctgatttgat acggacggta ttctgactat gacgcctcaa aaagggtgaa
      961 caatacgtga atctgaaaaa aaagagtggt aacggattac tccgcttctt ctttcctctt
     1021 aaatgtctcg acgagggtaa tttcgttgat ctccgggatg tgctcaagcg attccccgat
     1081 aacgcggctt ctccagagca cccaccgacg atcatagttg atacccggag ggagggtgat
     1141 cgtgagtatg ccatcagcaa acgcggtctc catgtcccgg ccggcataga ggcggatgag
     1201 cccgttgacc ttctcctcga ccgaggtcac ctggctttcg atcctgtacc agtaatggag
     1261 ctccttgccg gcaaaggggt ggttgaagtc aaggaggacc ctgttgccga tgacatcgac
     1321 aacggtcccc tctcccttct cctcaacgct gaggtgcata ccgagcctcg gcttgtcctt
     1381 gaaggcattc ttgttgaacg cctccacttt ctttggatca tgagggccga acgccttgtc
     1441 cggtccgaca tcgacctctc cttcaaatcc cacctctttt ccaacgaggg cttcgtcaag
     1501 gccgaggata acatggtggc tcccgacacg gatggtcacc gggccgtaca gtccctccgg
     1561 ggtgtagatc cctgcctcct ttgctacctc ttcatcggtg gtatcaaaaa cgctccccgc
     1621 gatactgccg gtataactca aggtaataaa gtctccttcc tgtagtgcca tgaactgaac
     1681 ctcttcctca tacaagggcg ggatatcaca ttattgtatc caccctggat caggaatggg
     1741 tgtatgccag ggtgcccatg cagtatcctc agccaggcgg agaggatgaa tccctgatag
     1801 tgtatcatgt cccgaggaat cggacgaaga agaaggcgtt gagtcggaag agatctgaca
     1861 ggacttttgc tgaaaaaaga gggatggccc taacgagacc ggtagaggat cccatccatg
     1921 cgacccgatt ttgggatcga cactgcagta gtgaacagct ccataaactc cgctgtggtc
     1981 tccgggaggg ttcgtgagac catggcaggc ttttcatagg acacaatatc agcatacccc
     2041 ggccgatctt cgatgggacg ggggtcatcc gggcagtaaa tcccatggat gggacagaca
     2101 taatccgcag gaatgggagt cccgtttgca aatgaataga gtgtggatcc gggtgcatta
     2161 ttcgatgata tgctaaggga ggagtacccg gttttcagcg aggattgtgt agttccacag
     2221 gtcgggcagc tgtccgggga gttcaggtct gattcgatac ctgtatcgaa tatgaaagaa
     2281 caggtggggc accccgcgta gggattgggg tcagggagcc cgtcatcatc ataggcgaag
     2341 acacaggtgg ggcaccctgc gaagggattg gtgtcaagga gcccgtcatc atcgtatgca
     2401 aagacgcaga caggacatcc ggcgtaggga ttgggatcga gaaggccatc attatcgtag
     2461 gcgaagacac agctggggca tcctgcatat ggatttgctt caatgagcaa gccataccag
     2521 tcataatcag atgcattccc ggaagccgat accgaacccg caagagagaa ccctatcagg
     2581 aagaggcaga tcaagaccgg tttcatacca atcagttata ttccgcagat acataaccat
     2641 tggtatcacc agaaccaccc gttctcctgc tccaaccaac aggctaacat agcgatgaag
     2701 agacatacct gtggaaacgg catgcttgaa gtcgagataa aagcccgggt tcctgacctt
     2761 gcacccatcc gggagcggct cctcaattgc catgagggcg ttcctgaacg ggttcatgag
     2821 agggacatct attacaactc cccccaccgg gattttcgcc agaccgacga agcgctccgg
     2881 ctgcgatacg ccgggggatc ctgcatcctc acctacaaag gtgagaaaag aaaggagttc
     2941 cggctcaagg ctagagagga actgaactgt gtcgtggaat caggggagat gatagagctg
     3001 atcctctccc gtcttggatt tttccccgca gctgaggtag agaaatggag ggaatattat
     3061 tcctaccgcg gagctaccgt atccctcgat gaggtgaagg gcctcggatc gtttgttgag
     3121 atcgaagcac cggggagtcc gggatacgac catcctgagg aattcatccg tgagatcgct
     3181 gaggagctcg gggtagaggg agagccgatc ctctcctcgt atctggagct gctccagtct
     3241 gcaccgtgat cagttcgatc tcgtgataga gggggaaccg atcctcacca tatatccgga
     3301 gctgctcccg tctagaccgt gatcagttcg atctcgtgat agagggggaa ccgatcctca
     3361 ccatatatcc ggagctgctc ccgtctagac cgtgatcagt tcgatctcga tctccttcgg
     3421 ttcgttgccg aagtggatca tggctccgtc gcccagcgcc gccatacccg ggttgactac
     3481 ttttaccccc tcttcgatgg cgatgccccg ctcctcgtgg atatgggcac agcagaccag
     3541 gtcgaagctg gtgaggtgac ggcgcacact ctggctcccg acatggttcc cgttggcctg
     3601 gtcgagaagg ccgtagggag gtgcatggct gatcaggacg ttgtgcatcg ccagttcgcg
     3661 cttttggagg accttgtgaa gaaggtcatc gatctgcttg tcggtcagtt caaagagcgt
     3721 gctgaagggg gtgggattcg accctccaat cccgcagatc gagatctttc caaggttgat
     3781 ctgtgcgcca tggaggcaga cacagtcgga ctgttccagg acatcgatcg tctcccgtgg
     3841 atcgcagtta ccaggaactg caaagcaagg aacatcgatt ctgtcaagaa cgtcggtcac
     3901 cgattcggca ggacccatgt tggtaagatc ccctgcaata aagactgcat cgggattcag
     3961 atcaaggaat ggatcgagtt ttccgtattc accgtgaagg tctgccagaa gcaggacatc
     4021 catcatgctc tatcattcga acatgaacca caaaaggata tccttggatc cctccatgat
     4081 ccggttactt tccccttcag tgcaagcgat ccgagcaggt gggagagctc agggatgagc
     4141 gggaccgggc tggtcttttc gagaggagtt cctggcaacg ggagaaagag gtgcgcgtgg
     4201 accgtcccat atgtactgat ccagcggatc agatcggccg tcgcgcgctg gtcttcgtca
     4261 gtctcaaaag gaaatcctac gatcacatcg accaccggct tgaggccagc gtcgcggcac
     4321 agctccaccg catggatgac atcatcgacc gagtgcccgc gcttcagatg ctcaagcacg
     4381 ctgtcactcc ctgactgggc gccgaactga agactggtgt tggcacaata cctggtgatc
     4441 agggagagtg cctcttcgga cacgaactcg ggccggacct cgctaggaaa cgtcccgaga
     4501 tagatattgt tccggagccg tttcaggagc cgttccacct tggcgaggca gggccgtctg
     4561 ccgtcggaac cataggcgag ggcattcgga gtgacgaacc gtgcatcccg gtaccgcgag
     4621 gcgtaccggg caacctcatc gatgctcctg tgccgcatgg catggccgaa gatccttggg
     4681 gtctggcaat aggcgcagga gaacgggcat cccctggata gctccacgta tcctttcatc
     4741 tgcgagaacg ggggccaggc atctagacgg accatgctgt cggcaggccg gaagccgtgg
     4801 cgggtggcta cgcccgggac cgtatcggcg gttccctttt cgatcgcaaa gaggagttcc
     4861 ggcagggtac gttcgccttc tccgacaacc acgtagtccg ccttctctgc cacttcccgg
     4921 tagcatgccg tagcgtaggg tcctcccgca atagtaatac agtcccccgc ttcaatctcc
     4981 gtaagaaggg aagtggcatt cagcgaactg aggctgtaac aggtgatatc aggggctggt
     5041 gcactgacag gcgaaagcag gataccctca cgttcacacg ccgcatagag ggcggcgtag
     5101 gtattcctgg tcccgctggt gaacctccag tgtaccttca tttctgtggt agtgtcttgt
     5161 ccgggataaa aaaaggaagg gggcgctatc cccgggtccg gtaaggcacg agctggtcga
     5221 tgatcttata ggtcttcccg gtattcagtg tcacatacac ctcgatccgg tcggactcct
     5281 tcgtcccctg gatatccacc tcgtcctgca tctcggccgt aaggtgctcg ataatcacct
     5341 ctccatccga ccgcgtgagg cggacaagga tatccctggt ggcgatctgt ccttctcccc
     5401 cggcaaagat gactttgatg gtcgctgtta tgggatcttt ctcgtttatc tggaccgtga
     5461 ctgactggcc gggcggcatg gtgacggtcg gacccggtgt catactgtcc ggttggctgc
     5521 ccgggggtat tccgggtata ctttccgtgc agcctgcaca gaggagagcc atagccgaca
     5581 gaaggatgaa gaacagaacc tggttctgca taacagagac tctataaatc gaacaggttt
     5641 aaaaaattgt tcaacctggg ggttcagggt atgccatcct gatcgtattc gatcgttccc
     5701 gtatcgccat ctacggtgat gcaggtcccg ttccggagtt gcatgatgtc ctcttcaggt
     5761 tggtctacca tggggatctt gcccatgatc gcacctacag caatgatggg atccgccctt
     5821 gtattgatga tgcccgcagg ggcttttttg ttcctcttca gggcatagag gacatacgat
     5881 cctaccgtcg atcctttccc atgtgggaag gcgaggacct ttccggtgat gctctcccct
     5941 tca
//
LOCUS       DAOOCO010000121         5865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12820_length_5865_cov_4.767986, whole genome shotgun sequence.
ACCESSION   DAOOCO010000121 DAOOCO010000000
VERSION     DAOOCO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12820_length_5865_cov_4.767986"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="prs"
                     /locus_tag="PK154_07360"
     CDS             <1..670
                     /gene="prs"
                     /locus_tag="PK154_07360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPW10915.1"
                     /translation="VQLILLIDACQGADITLVLPYLGYARQDKQFSAGEPLSARAVAG
                     VLSRGVSRAFVVNVHDPAVLRFFDVPAYTLSLAPDIGAFLKGTGCDDPLILAPDDGAA
                     RFASAVAGVNGWDVDHLEKTRLSSEEVSISPKNLCAEDRDVVIVDDIISTGGTLATAA
                     RLLYGQGARSVSAACVHGVFAGGAYAHLISSGVRQVYCSDTIERGCSVYSAAGIISDG
                     ITQC"
     gene            664..1641
                     /gene="rtcA"
                     /locus_tag="PK154_07365"
     CDS             664..1641
                     /gene="rtcA"
                     /locus_tag="PK154_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HPW10916.1"
                     /translation="MLRIDGSHLEGGGQIVRSAVALSAVTGIPIEITRIRAKRKKAGL
                     RFQHCAAVRAVACTCEGDTEGNEPGSDRLLFSPGPIRRSSCVVDVGTAGSIALVLQSW
                     LPVALTRGGTLEVTGGTEVPASPTIDYSENVLIPALGVPEGAVSVTITRRGYYPAGGG
                     LVRVRVDPQHPGPISLEKDPSCGIRSCSSGLPAHVAERQAASAQTMLSGALGIEVPIE
                     IQRSEGRSKGSSCTAWYGSKGFSSLGSPGFPAEEVGRVAAAGLISEVNRPGEADPCLS
                     DQLLISLARCGGSFTTSSYTLHARTMCWLLALFGYEIQVREGREVVFSA"
     gene            1638..2108
                     /locus_tag="PK154_07370"
     CDS             1638..2108
                     /locus_tag="PK154_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HPW10917.1"
                     /translation="MRLVLDSSAFFAGVSLEGSLFGGELCTTPSVVSELRDLSSKARF
                     DLLCERGLRVIDPETESVRQVREVAVAMGEGSSLSTTDCEVLALALQCEAAVMSDDYA
                     LQNVGRSLHLRIIPLHQKGGRNIRWKFWCSGCGRYYGGPGECKVCGALIQRRLK"
     gene            2132..2752
                     /locus_tag="PK154_07375"
     CDS             2132..2752
                     /locus_tag="PK154_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HPW10918.1"
                     /translation="MSSLDELIERARALLSEGHSPGQIADELSLSIETVTWLLTQQKS
                     GAIPKDVGIDWTAVSSEGPLLDELAMMMLKHFYSAISDEARGEEQPELFPSAVVGIAI
                     SGIPLATLIAVSEDVRLAIYYPAKQSTHDPPVGSISGNFAPISGERCLIVDDVITSGN
                     TMHEVVHYVRKHGGNPVAVLVIFDKRGIRDVDGVPVYSLFRISRLD"
     gene            2836..4428
                     /gene="thsA"
                     /locus_tag="PK154_07380"
     CDS             2836..4428
                     /gene="thsA"
                     /locus_tag="PK154_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HPW10919.1"
                     /translation="MLGGQPIIILKENVERNRGQEAQRSNIAAAKAIASAVRTTLGPR
                     GMDKMLVSGTGDVTITNDGATILTDIAVQHPGAKMVIEVARAQDDEVGDGTTTAVVLV
                     GSLMEQAEHLIAKKIHPTVIAQGYRMGMEKALEIISDLSISIDSYDRDILLKIADTAM
                     TGKSIESVKKKLDGIVVDAVMLVAEKKDGKVVVDDDHITIKKQAGHAMDDAELVQGIV
                     LDKTRFAEDMPRKVTGAKVALVASPMEIVKTQVKAKIKISSSDQISAFSAQEREALKK
                     LADTVIESGANVLFCQKGIADAVGYYLARAGVLAVEDVPEKDIKFAALALNAEIVNKP
                     GELTKKSLGTAAMVEQVDNADLIKVSGCKNPKAVTILLRGSTEYLLDELERAVVDGTR
                     VVEDAMEDGKLLVGGGAVETELLMKLHEYAGTVGGRVQLAIEAYAASFEIIPTTLAEN
                     SGFNQLDKLVELKAAHAKGKKHAGLNVYTGKIIDMFKEGVVEPHRSKRQSIQSSAEAA
                     VLLLRVDDMMITQEKSPGSAGA"
     gene            complement(4456..4656)
                     /locus_tag="PK154_07385"
     CDS             complement(4456..4656)
                     /locus_tag="PK154_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine S protein"
                     /protein_id="HPW10920.1"
                     /translation="MAIQSRMRLLPTDEVKIYEGMEGDTYEKALLSFGINPDSVLILF
                     RGTSIPQDSEIREDQVEIVCIW"
     gene            4702..4786
                     /locus_tag="PK154_07390"
     tRNA            4702..4786
                     /locus_tag="PK154_07390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4736..4738,aa:Ser,seq:cga)
     gene            5003..5488
                     /locus_tag="PK154_07395"
     CDS             5003..5488
                     /locus_tag="PK154_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10921.1"
                     /translation="MKGTILVILLLTAVTLLVSGCTDSSAPPESLTPVTTIPLTSPLV
                     TPTITQAIADYLTGPMPGDKSVAISVDRSVVNPNITITFRGGAGINFVATMDTVLTRS
                     DGVVMQDSLPRPRVNDQITMIGSKEVSEMRADRLQVFITLVTGDKYLIYDQTLPYKSR
                     G"
     gene            complement(5621..>5865)
                     /locus_tag="PK154_07400"
     CDS             complement(5621..>5865)
                     /locus_tag="PK154_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10922.1"
                     /translation="GPVHQVAVSATITYLSPAKGDLEAVAEQVSETEQHSLFLVRVYD
                     GKRLVATFDGVGIKEKRAKGARSRPSLGEDIPLKTG"
BASE COUNT         1287 a         1653 c         1672 g         1253 t
ORIGIN      
        1 tgttcagctc atccttctca ttgatgcctg ccagggagcc gacatcaccc tggtcctgcc
       61 gtacttggga tacgcccgtc aggacaagca gttctcggca ggagaacccc tctctgcccg
      121 ggcggtcgcc ggagttctct caaggggagt atcaagggca tttgtcgtga acgtgcatga
      181 tccggcagtc ctgagatttt tcgacgtccc ggcctatacc ctctcccttg cccctgatat
      241 cggggcattc ctgaaaggga cagggtgtga tgacccgctc atccttgccc cggatgatgg
      301 tgctgcccgg ttcgcatcag cagtcgcggg ggtgaatggc tgggatgtcg atcatctcga
      361 aaagacccgc ctctcttcag aagaggtgag catatcaccc aagaacctct gcgctgaaga
      421 tcgcgatgtt gtcattgttg acgatatcat ctctacgggg ggaacgctgg caaccgctgc
      481 acggctcctg tacggacagg gagcacggag tgtctctgcg gcatgtgttc acggagtgtt
      541 tgccggagga gcgtacgctc atctgatctc atcaggagtg aggcaggtat actgcagcga
      601 taccatcgag aggggttgca gtgtctattc tgccgcgggg atcatctctg acggaatcac
      661 ccaatgctaa ggattgacgg ctcacatctt gaaggcgggg ggcagatcgt ccgatctgcg
      721 gttgcgctct cggcagtgac aggaattccg atcgagatca ccaggataag agcgaaacgg
      781 aagaaggccg gactcaggtt ccagcactgc gctgccgtcc gggcggtagc ctgtacctgc
      841 gagggagata cggagggaaa tgaaccgggg agcgaccggc tcctgttctc tcccggcccg
      901 atccgcaggt cctcgtgcgt tgtcgatgtc gggacagcgg ggagcattgc ccttgtactc
      961 cagtcctggc tcccggttgc cctgaccagg ggcgggaccc ttgaagtgac cgggggcact
     1021 gaagtacctg caagccctac catcgattac agtgagaacg ttctgatacc tgctctgggg
     1081 gtacctgagg gggcagtttc agtcacgatc acccggaggg gctattaccc ggctggggga
     1141 ggcctcgtcc gggtccgggt agatccccag caccccggac cgatctctct tgaaaaagat
     1201 ccttcctgcg gtatccgctc ctgctcgtcc gggctccctg cccatgtcgc tgaacggcag
     1261 gcagccagtg cgcaaacgat gctatccggt gcactgggga tcgaggtccc gattgagatc
     1321 cagcgttcag aagggagatc gaagggcagt tcctgtactg catggtatgg atcgaaagga
     1381 ttctcatcac tcggcagtcc cggttttccc gccgaagagg tcggcagggt cgcagcagcc
     1441 ggcctgatca gcgaagtgaa cagaccggga gaggcagatc cctgcctgag tgaccagctc
     1501 ctgatctccc ttgcccgttg cggtggatca tttacaacct cctcgtatac cctccatgca
     1561 aggacgatgt gctggctcct tgcgctcttc ggctatgaaa tacaggtcag ggaaggccgg
     1621 gaggtggtct tttccgcatg agactggtcc tcgactccag cgcgttcttt gccggggttt
     1681 cgcttgaggg atcactgttt ggaggagaat tgtgtacgac cccttcggta gtttcggaac
     1741 tgcgggatct ctcttcaaaa gccaggttcg accttctctg cgagagaggg ctccgggtga
     1801 tcgatccgga gacggaatcg gtgcgccagg tcagagaggt cgctgtcgca atgggggagg
     1861 ggtcatccct ttccactacc gactgtgaag tgctcgccct cgctctccag tgcgaggctg
     1921 ccgtcatgag cgatgactat gcgctccaga atgtgggaag gtccctgcac ctcaggataa
     1981 tccctctcca tcagaagggg gggcggaaca tccggtggaa gttttggtgc agcggatgcg
     2041 gcaggtacta tgggggacca ggggaatgca aggtctgcgg ggccctcatc caaagaaggc
     2101 ttaagtaaag gggcgcccac actctttttc aatgtcatct cttgatgagc tgatcgagag
     2161 ggcacgggcc ctcctctccg aaggccatag tccgggacag attgcagatg agctctccct
     2221 ctccattgag acggtaacct ggctcctgac gcagcagaag tcgggggcga tcccgaagga
     2281 tgtcggcatc gactggactg cagtgagcag cgaagggccg ctccttgacg agcttgccat
     2341 gatgatgctg aaacactttt actcagcgat atcggatgag gcgcggggag aagaacagcc
     2401 ggaattgttc ccctcagccg tggtggggat tgcaatatcc gggattccgc ttgcgaccct
     2461 gatcgcggta tccgaggatg tacggctcgc aatctattat ccggcaaagc agagtaccca
     2521 tgatcccccc gtcggatcga tcagcggcaa ctttgctccc atttccggtg aacgctgcct
     2581 gatcgtggac gatgtgatca cctcagggaa taccatgcat gaagtggtcc actatgtccg
     2641 caaacatgga ggcaacccgg ttgccgtgct cgtgatcttt gacaagcgcg ggatcaggga
     2701 tgtggatggc gtgccggttt attcgctctt caggatctcc agactcgact gataccactc
     2761 tcttttcttt cacctcgtat ggattgtttt atatagaagt tcaattccat cttttaccac
     2821 attggaggat aacctatgct tggaggacaa ccgataatta ttctcaagga gaatgtcgag
     2881 cgcaaccgcg ggcaggaggc ccagcgttcg aacatcgccg ctgctaaagc catcgcaagt
     2941 gccgttcgca ccacgcttgg tccgcggggg atggacaaga tgctcgtctc aggaacgggg
     3001 gatgtcacca tcaccaatga cggtgccacc atccttaccg acatcgccgt ccagcacccg
     3061 ggcgccaaga tggtgatcga ggtcgccagg gcccaggatg acgaggtcgg ggacgggact
     3121 accaccgctg tcgttctcgt gggatcgctg atggagcagg cagaacatct catcgcaaag
     3181 aagatccatc ccaccgtgat cgcacaggga taccggatgg ggatggagaa agccctggag
     3241 attatcagcg atctctctat ctccatcgat tcctatgacc gggatatcct cctcaagatc
     3301 gcggataccg cgatgaccgg caaatcgatc gaatccgtga agaagaagct tgatggaatc
     3361 gtcgtcgatg ccgtcatgct ggtcgccgag aagaaggacg gcaaagtcgt ggtcgatgac
     3421 gatcatatca cgatcaagaa gcaggccggg catgccatgg atgacgccga actggtccag
     3481 ggaattgtcc tcgacaagac ccggtttgct gaggatatgc cacgcaaggt gacaggagca
     3541 aaggttgcac ttgttgcaag cccgatggag atcgtaaaga cccaggtcaa ggcgaagatc
     3601 aagatcagca gcagcgatca gatcagtgcc ttctcggcgc aggaacgtga agcgctgaag
     3661 aaactggccg atacggtgat cgagagcggg gcaaatgtgc tcttctgcca gaagggaatt
     3721 gccgatgcag tgggctatta ccttgcccgt gccggtgtcc ttgctgtcga agatgttcct
     3781 gaaaaggaca tcaagttcgc cgcccttgcc ctcaatgccg agatcgtcaa caaaccaggg
     3841 gaacttacca aaaagagcct tggaactgcc gcgatggtgg agcaggtcga caacgccgac
     3901 cttatcaagg tctccgggtg caagaacccg aaggcggtta cgatcctcct tcggggctca
     3961 accgagtacc tcctcgatga acttgaacgt gcggtagtgg acggcacccg ggtcgttgag
     4021 gatgcgatgg aagacgggaa gctccttgtc ggaggaggcg ccgttgagac cgagctcctc
     4081 atgaagctcc acgaatatgc cgggacggtc gggggacggg ttcagctcgc catcgaggcg
     4141 tacgcagcat cctttgagat catcccgacc acccttgcgg agaactcggg attcaaccag
     4201 ctcgataaac tggtcgaact gaaggcagcc catgcaaaag ggaagaagca tgcagggctc
     4261 aatgtctata ccgggaagat catcgatatg ttcaaggaag gagtcgttga accccaccgg
     4321 tccaagcgcc agtctatcca gagttcggca gaagcggcag tgctcctgct ccgggtcgat
     4381 gatatgatga tcacccagga gaagtcccca ggctccgcag gggcataacc ttcccctctc
     4441 ttttttatac cccttttacc agatacagac gatctcgacc tggtcttccc ggatctcgct
     4501 gtcctggggg atactggttc ccctgaacaa gatcaatacg gaatccggat tgatcccaaa
     4561 cgagaggagg gccttttcgt aggtatcccc ttccatcccc tcatatatct tcacctcgtc
     4621 agttggaagg agtctcatcc tgctctgtat cgccattcga taccattata cgggttccag
     4681 agaaaaatat ctggtccctg tgccgaggta gtctagcccg gaaaggcgat ggtctcgaaa
     4741 accattggcg ccctgcgcct cgggagttca aatctccccc tcggcgtatc ggatcttctc
     4801 ttcaagcctt tatcgaacaa agacccttgc cctcaatgat ctgttcttgt ccctctcctc
     4861 tcttcccaga caatcccgca gaacagagat ccataaagat tactcctccc cctgccccga
     4921 catcataaac agaaattggt acaaatgagc ctatatgcac tgaaaaaagc ctataataca
     4981 gaagcccttc ctatctttct gcatgaaagg aacaatactc gtcatcctcc ttctgacagc
     5041 agtcaccctc ctggtgtccg gttgcaccga ttcttcagca ccaccggaat ccctgacccc
     5101 tgtcacgaca atacctctga cgtctcctct cgttactccc acaataacac aggcaattgc
     5161 cgattacctg acgggaccca tgcccgggga caaatcggtc gcaatcagtg tcgaccggag
     5221 tgtcgtgaac ccgaacatta cgatcacctt ccgcggggga gccgggatca acttcgtagc
     5281 gacgatggat accgttctca cccggtctga cggggttgtt atgcaggaca gtctcccgag
     5341 acccagggtc aatgaccaga tcaccatgat cgggtcaaag gaggtttcag aaatgcgagc
     5401 tgaccggctc caggtattca tcacgctcgt gactggtgac aagtacctga tctatgacca
     5461 gaccctcccc tacaagagcc gggggtaggt ccattttttg ggacggtatc cctcaccagc
     5521 agctgggaaa gaaccacccg gggaatctcc ccgtgtctga tgaggcaccc tgccctgctc
     5581 ctgggaggta aagggtatcc ggtccggttc caatagaaga tcaaccggtt ttcaggggga
     5641 tatcttcccc cagtgaaggc cgtgaccgtg ccccctttgc cctcttctcc tttatcccga
     5701 caccatcaaa cgtagcaacc agtcgtttcc catcatatac tctgacaagg aagagcgaat
     5761 gctgttccgt ctcggagacc tgctcggcaa ctgcttcgag atcccccttt gcaggagaga
     5821 ggtaggtgat cgttgccgat accgcgacct ggtggacagg tccgg
//
LOCUS       DAOOCO010000122         5840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_12907_length_5840_cov_5.281936, whole genome shotgun sequence.
ACCESSION   DAOOCO010000122 DAOOCO010000000
VERSION     DAOOCO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12907_length_5840_cov_5.281936"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..85)
                     /locus_tag="PK154_07405"
     tRNA            complement(1..85)
                     /locus_tag="PK154_07405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:gag)
     gene            272..772
                     /locus_tag="PK154_07410"
     CDS             272..772
                     /locus_tag="PK154_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HPW10923.1"
                     /translation="MWAAIISEFADSPSQSRVARFLLENGFGVNPEGRIECNGIEVSA
                     TKVAKAIGTDRRVVDATARHILESPSLREIFANIRATPDLSLLAGPLGLSVITVIPKD
                     ARQKGIVGAAVEVVTTHNLNIRQIFVTDPVLSEEPRLVIIIDGTLPGVVIEEIKSLPQ
                     VKRVIL"
     gene            complement(833..1348)
                     /locus_tag="PK154_07415"
     CDS             complement(833..1348)
                     /locus_tag="PK154_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPW10924.1"
                     /translation="MREDDSRIDTLLREAGCSMRVIAHCHAVRDLALASADGAGVLNR
                     ELLNAGALLHDIGRGFTHSLHHAQAGAAYLRDAGISPEIVRIVERHIGAGLTSDECTL
                     LGILPIDCMPRTGEEKVVANADNLVRGSRVVSIYDRLESSRYLGRKIMFRTYRLWLFC
                     EQIRRTPAPEK"
     gene            complement(1341..1853)
                     /locus_tag="PK154_07420"
     CDS             complement(1341..1853)
                     /locus_tag="PK154_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HPW10925.1"
                     /translation="MDEILEQPAVKAYLLRLIGEEGMALLQKFPEEGEWSDEQLAAQT
                     GINLNSVRHTLYTLYEKRLAEYRRIKNNETGWLTYLWHLRTDSIFDVIREDLETILEK
                     LKTREKYEEENDFYICKSCGVIVTFNQAFDVSFVCPACDLQLEHYENEMLLRALKKRI
                     EEIQETLGHA"
     gene            complement(1846..2925)
                     /locus_tag="PK154_07425"
     CDS             complement(1846..2925)
                     /locus_tag="PK154_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPW10926.1"
                     /translation="MKVLVAGFATRHIAQSAHAAGYEVYSVDHFCDQDLGFYVRESRS
                     FDDLGDLEGVVVEMCERVSPDLVIVSSGAELIGTRELCGTPPERVERFLDKVSTHDFF
                     EELGIPVPGLLPPGTYPAMMKPRKGSGGWRNAIVNNDAEFIEWDGKFGDLPYLAEEVV
                     GGVPSSVCCLGTGSSAMAVAVNRQILRGGGEAAFGFSGSVTPFCNDAAPFMVRYAERI
                     ASASGCVGTIGIDFMTGDTVHAIEVNPRFQATLDTIEMATGFNLFGAHVGACHGTLPS
                     RRPACRQVAARKILFAEKDMEISRNLVRFSPLIADIPWPGTSFEEGQVVLSVYGWGAG
                     EEEVEAMLDKHIRIVQQYMGGRLYG"
     gene            complement(2922..3446)
                     /locus_tag="PK154_07430"
     CDS             complement(2922..3446)
                     /locus_tag="PK154_07430"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HPW10927.1"
                     /translation="MPEIVVLRIGHRPERDQRVTTHVGLAARALGAKGMYLAADDKGV
                     VRSITDVVERWGGDFFIEDKVSWKRCIREWKESGGCVVHLTMYGERFLDREEEIKSRE
                     KILVVVGAEKVPGELYGLADYNIGITGQPHSEISSLAVFLDHLFSGAELEHEFPGAAI
                     RVVPSPLGKHTEES"
     gene            complement(3449..3727)
                     /locus_tag="PK154_07435"
     CDS             complement(3449..3727)
                     /locus_tag="PK154_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10928.1"
                     /translation="MKKEDLDWWIYHLLADTPDQDIEALAIATGCPGDALADSLDRLG
                     TTFLIEGCNGRYRIRSMHEMLLSCQAKYDETTPFTIENGIIRERKRKE"
     gene            3821..4228
                     /locus_tag="PK154_07440"
     CDS             3821..4228
                     /locus_tag="PK154_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10929.1"
                     /translation="MNDTTSVSFSELARIADQILEKAEDDPEILAQELDGLAPGIRNE
                     LLTSDLLNAYQVFFYFFREDPGDLEQDRLTLQPASALMTGVMMSETELFELFFSIEAG
                     EPVFSVSDGDRVVARFRGADAYQKALQFLDESF"
     gene            complement(4242..4952)
                     /locus_tag="PK154_07445"
     CDS             complement(4242..4952)
                     /locus_tag="PK154_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPW10930.1"
                     /translation="MIRHRFHLRETIATILADEEQHVEAARQGITRARQELEHYIAGD
                     PFFLTTFEPYSVDTGILVVDRMSGASGAAGVGPMAAVAGSIAWAGVEAMEEAGASFGI
                     VDNGGDIALLCDREVRIGIHAGSSPLSDRYAFVTGPLPGIWGICTSSATVGHSISLGV
                     ADAAVVFSQSPALADAWATAICNRVRKDDTAVVDEALLSGIEGACIIIGEWRYRKGTL
                     PKVVKAHVRHDLITGGLW"
     gene            5055..>5840
                     /locus_tag="PK154_07450"
     CDS             5055..>5840
                     /locus_tag="PK154_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10931.1"
                     /translation="MSMGIGGLRAQRSLRIGTRKAADYIIMARLRDQALGELISHFPA
                     LTGMSSGEARELHVTLFGPFRSRINGVTLLTRVEEGAGDLNRLTFRIGDLIRLKGLRG
                     GAIAMSLAPDQEFSAFYQRLVSAFSPLTEWCTWIDTPPGHRIFHVSLRFNIPFRDFDL
                     FWERTLGITPSQEYEPGEGRSPIPAVRTYLKACDSPVSIFRIAIMRRGSLWREYDLPQ
                     KTWLSRRESADTGEWERTYQTFWQNDGLELPESKHSTPSRQFLI"
BASE COUNT         1268 a         1798 c         1562 g         1212 t
ORIGIN      
        1 tgcgagggaa gggatttgaa ccctagaact cctacgagac ggacccctca agcccgcgcc
       61 tttgacctgg ctcggcaacc ctcgcacgtg ccataataca aacgacgttt atctaaaaaa
      121 gggtatccat ccttctcgtg ggagccggtc cctggtcctc cgggtccagt cagtactgag
      181 gacaaccagt agatatgctg catctgttca ctgatgccca tgaaaaagca gtgatgtata
      241 agaaccggca gcccgtatac tctcctttag tatgtgggct gccatcatca gcgagttcgc
      301 agattcgccc tcccagagca gggttgcccg attcttgctc gagaacggtt tcggagtgaa
      361 ccccgagggt aggatcgaat gcaacggcat cgaggtgtct gcaacgaaag tggcaaaggc
      421 gatagggact gaccggaggg tagtggacgc tacggcccgc catatcctcg agtcaccatc
      481 tttaagagag atctttgcca acatcagggc aacccccgac ctctcgcttc tggccggacc
      541 actcggactc tcggttatca ccgtcatccc gaaagacgcc cggcagaagg gaatcgtagg
      601 agctgcagtg gaggtcgtca ccacccataa cctgaatatc cggcaaatat tcgtgaccga
      661 cccggtgctc tcggaagaac cgaggctggt gatcatcatc gatggcactc tccccggtgt
      721 ggtgatagag gaaataaaaa gcctcccaca ggtaaagagg gtcatccttt aggtgaaacg
      781 ggtcatcctt cgggtgaacc gtccatcttt aaaaaaaagg gaaccgaccc cttcactttt
      841 cgggggcagg ggttcgccgg atctgttcac aaaacagcca caaccgatag gtccggaaca
      901 tgatcttccg tccaagatac cgtgacgatt ccagccggtc atagatactg accaccctgc
      961 ttcccctgac aagattgtca gcattggcaa cgaccttctc ctctcctgta cgtggcatgc
     1021 agtcgatggg gaggatccca agaagggtac attcatcgga ggtcagtccg gcaccgatgt
     1081 gacgttcaac aatcctcaca atctcgggag agatcccggc gtcccggaga taggctgcgc
     1141 ctgcctgggc atggtgcagg gaatgggtga agccccgccc gatatcatga agaagggctc
     1201 cggcattcag gagctcgcga ttgaggactc ccgccccatc agcagaggca agggcgagat
     1261 cgcggacggc gtggcaatgg gcgatcaccc gcatgctgca gccggcttcc ctgagaagtg
     1321 tatcgatccg tgaatcgtcc tcacgcatga ccgagggttt cttggatctc ctctatcctt
     1381 ttcttgaggg cacggaggag catctcgttt tcatagtgct cgagctgcag gtcacaggca
     1441 gggcagacaa aggagacatc gaatgcctgg ttgaaggtca cgatcacacc gcaggacttg
     1501 cagatgtaaa agtcgttctc ctcctcgtat ttctcccgcg tcttcagttt ctcgaggatg
     1561 gtttcaaggt cttcccggat cacatcaaat atcgagtcgg tgcgcaggtg ccagagatag
     1621 gtcagccagc ccgtctcgtt attcttgatc ctacggtatt ccgcgagccg cttctcgtag
     1681 agggtataca gggtatggcg taccgaattc aggttgatcc cggtctgggc agcgagctgt
     1741 tcatcgctcc actctccctc ctctgggaat ttctggagga gcgccatccc ctcttcaccg
     1801 atcaagcgca aaaggtatgc ctttaccgcc ggttgttcca ggatctcatc cataaagcct
     1861 tccacccata tattgttgta ctattctaat atgcttatcc agcatcgcct ccacctcttc
     1921 ctcccccgct ccccatccgt agacactcag caccacctgt ccttcctcga acgatgtccc
     1981 cggccaggga atatcggcaa tcagcggtga aaaccgaacc aggttccggc tgatctccat
     2041 gtccttttcg gcaaagagta tctttcgtgc ggccacctgg cggcatgcag gccgtcggga
     2101 ggggagtgtt ccgtggcatg caccaacatg cgctccaaag aggttgaatc ctgttgccat
     2161 ctcgattgta tcaagcgttg cctggaaccg gggattgacc tcgatcgcgt ggaccgtatc
     2221 cccggtcatg aaatcgatcc cgatcgtgcc tacgcagcca ctcgctgatg cgatccgctc
     2281 ggcataccgc accatgaacg gcgcggcatc attacaaaac ggggtcaccg agcctgagaa
     2341 cccgaacgcc gcttccccgc ctccccgaag gatctggcga ttgacggcaa ccgccatggc
     2401 agatgacccg gtccccaggc agcagaccga tgacggaacc ccaccgacca cctcctcggc
     2461 gagatacggc aggtcaccga atttcccgtc ccactcgatg aattcagcat cattgttgac
     2521 gatggcattc ctccaccctc ccgaaccctt ccgcggtttc atcatggccg ggtaggtccc
     2581 cggggggagg agcccgggaa cagggattcc cagttcctcg aaaaaatcgt gagtagagac
     2641 cttgtccagg aaccgctcca ccctttcagg gggagtacca cagagttccc ttgtgccgat
     2701 cagctcggca ccggatgaaa caatgacgag atccggggag acccgctcac acatctccac
     2761 aacaacccct tccaggtcgc caagatcatc aaacgaccgc gattcccgga catagaaccc
     2821 gaggtcctgg tcgcagaagt gatcaacgga gtagacctcg tagccggcag catgggcaga
     2881 ctgggcaata tgccgggtgg caaaaccggc cacgagtacc ttcatgactc ctcggtatgc
     2941 tttcccagag gggaaggaac gacccggatc gctgcacctg gaaactcgtg ctcgagttct
     3001 gccccggaga agagatggtc caaaaagacg gcaaggcttg agatctccga atgaggctgg
     3061 ccggtgatcc ctatgttata atccgccagg ccatacagtt caccagggac cttctcggca
     3121 ccgaccacca cgagtatctt ctcccttgac tttatctcct cctccctgtc caaaaagcgc
     3181 tccccgtaca tcgtcaggtg gaccacgcac cctcctgact ccttccactc cctgatgcac
     3241 cgcttccacg agaccttgtc ctcgatgaaa aagtcgccgc cccagcgttc gaccacgtca
     3301 gtaatactcc ggacgactcc cttgtcatcg gctgcaagat acatcccctt cgcgccgagc
     3361 gcccgggctg ccagcccgac atgagtggtt acccgctggt cgcgttcagg acggtgcccg
     3421 atccggagca ccacgatctc cggcattgtc actccttcct cttccgctcc ctgatgatcc
     3481 cgttctcgat ggtaaacggg gtggtctcat cgtacttcgc ctggcatgaa aggagcatct
     3541 catgcatcga acggatccgg tatctcccgt tgcagccttc gatgaggaat gttgttccaa
     3601 gacgatcgag ggaatcggca agagcgtcgc cggggcagcc ggttgcgatg gcaagtgcct
     3661 cgatatcctg atctggtgtg tctgcaagga ggtggtagat ccaccagtcc agatcctctt
     3721 ttttcacctt tacacattcc gcgggtcgat gattataggt tttcgttccg ccagggaaag
     3781 cgggtattat atacaggaaa actccgatac gattgataca ttgaacgata ccacctccgt
     3841 ttcattcagc gagcttgccc gcatagccga ccagatactc gagaaggcag aggatgatcc
     3901 ggaaatcctt gcacaggaac tggacgggct tgctcccggg atccggaacg agctcctcac
     3961 ctcggatctc ctcaacgcgt accaggtctt cttctacttc ttcagggagg atcctggtga
     4021 tctcgagcag gacaggctca ccctccagcc ggcgtcggca ctcatgaccg gggtgatgat
     4081 gagcgagacc gagctttttg agctgttctt ctccatcgaa gcaggggaac cggtcttttc
     4141 agtgagcgac ggagaccggg tcgttgcccg attccgggga gctgatgcct accaaaaggc
     4201 actccaattt ctcgatgaat cgttctgaca cagttctctt cttaccagag ccctcccgtg
     4261 atgagatcgt gccgcacgtg tgctttcaca accttcggga gagttccctt ccggtaccgc
     4321 cactccccaa tgatgatgca ggctccctcg atcccgctaa gaagcgcctc atcgaccacc
     4381 gcagtgtcat ccttcctcac ccggttacag atcgcggtcg cccaggcatc agcgagcgcc
     4441 gggctctggg aaaatacgac ggccgcatcc gcgacaccga gagagataga gtgcccgacg
     4501 gtcgctgagg aggtgcagat cccccatatc cctgggagag gcccggtgac aaaggcatac
     4561 ctgtcggaga gtggcgagct gccggcatgg atcccgattc gcacctcccg gtcacaaagg
     4621 agcgcgatat ccccgccatt gtccacgatc ccgaaggagg cccccgcttc ctccatcgct
     4681 tcaacccctg cccaggcaat cgaccctgcc accgctgcca tcggccccac ccctgcggct
     4741 ccggacgcgc cggacattcg gtcaaccacc aggatcccgg tatcaacaga atatggctca
     4801 aaggtggtga gaaaaaaggg atcccccgcg atatagtgct ccagttcctg ccgggcacgg
     4861 gttattccct gcctggccgc ctccacgtgc tgttcctcgt cggcaaggat ggtggcgatg
     4921 gtctcgcgga ggtggaaccg gtgcctgatc ataggagtac ttctctcttt tcacgagaca
     4981 aatacccgtc gaaccggaaa cgccctgacc aaacagggag ctatttatcc gatcatgagt
     5041 gagcctttca tgaagtgagt atgggcattg gagggctcag ggcccaacgg tcattgcgga
     5101 tcggcacccg gaaagcagcc gattatatca tcatggcccg gctccgggac caggccttgg
     5161 gcgaactgat cagccacttt cccgcactta ccgggatgtc atccggtgag gcacgcgaac
     5221 tccatgtcac cctctttggc ccgttccgtt cccgtatcaa tggcgtaaca ctcctcaccc
     5281 gggtcgaaga gggagcggga gacctgaacc ggttgacctt cagaataggt gatctgatcc
     5341 ggctgaaagg tttgcggggc ggggctatcg ccatgagcct tgcacctgac caggaatttt
     5401 ccgcgtttta ccagcggctg gtatcggcct tttccccatt gacagaatgg tgcacctgga
     5461 tcgacacccc ccccggccac cgtatcttcc acgtctctct ccgcttcaac attcccttcc
     5521 gcgatttcga cctgttctgg gagaggacgc ttgggatcac tccatcgcaa gagtacgaac
     5581 ctggggaagg gaggagcccc atccctgctg tccggaccta cctcaaagcc tgtgattccc
     5641 cggtcagcat attccggata gcgatcatgc gcagaggatc gctctggaga gagtacgatc
     5701 tcccgcaaaa gacctggctc tcaaggagag aatcggcaga cacgggcgaa tgggagagga
     5761 cctaccagac gttctggcag aacgacgggc tggagctgcc cgagagcaag catagcactc
     5821 catcccggca gttcctgatc
//
LOCUS       DAOOCO010000123         5776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13112_length_5776_cov_4.637651, whole genome shotgun sequence.
ACCESSION   DAOOCO010000123 DAOOCO010000000
VERSION     DAOOCO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_5776_cov_4.637651"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK154_07455"
     CDS             complement(<1..384)
                     /locus_tag="PK154_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW10932.1"
                     /translation="MEFERIPTVPTADEILDRSFRRAASKMRLKRNKDRANEEFVRAV
                     SHGIHDRLVQIIRSFPELNDLPPFYRDIVDIMWGMDRVKKSLGAVGWAARWARNHGPG
                     LAYQTRKAETPSVIRKRTVARLSSVV"
     gene            433..1311
                     /locus_tag="PK154_07460"
     CDS             433..1311
                     /locus_tag="PK154_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW10933.1"
                     /translation="MDLSLIQKDILITLITLYHQHSHPIKGEEIADVIKRNPGTVRNQ
                     MQALKAIGLVDGIPGPKGGYNPTSLAYKELNLGISEGEYDVSISRDGEVVTGVKVVEI
                     AFTTLCHPDICQASVRIIGSVRAFEIGDNITIGPTPVNKLLIRGEVYGKDEISQTLLI
                     STYEMISLPKKQIRYYMSAPLITLKGTDSIADAIRLFTTHHIHGAPVMENGRLAGIVT
                     MSDIAKAIGNHKTLDEKVAAVMTTDVVKAPSNVQLFEVIRRFKEREIGRLVVIEDDRP
                     VGILTQSDIIRQFPTL"
     gene            1444..1695
                     /locus_tag="PK154_07465"
     CDS             1444..1695
                     /locus_tag="PK154_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPW10934.1"
                     /translation="MARVLARSLSKKKIVTNEGKVIGTLKNLVVDFETGQVVDMIVQP
                     DPSFDTSGYRVEGDRMFVSFESVKDIKDYIIVDRYLSRK"
     gene            complement(1677..2375)
                     /locus_tag="PK154_07470"
     CDS             complement(1677..2375)
                     /locus_tag="PK154_07470"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HPW10935.1"
                     /translation="MLKGLVTDIDGTLTDRHRRIHTGAIEYIRKLCDQGILVVLASGN
                     TSCFMDALSKMIGTQGAFIAENGGVYRTGYAQPLRILGNQTVCKEALKAVEDFYSRSG
                     ITLDLYSPTYRFADLAFARTVPVDEVREIVKDFPVDVLDTGFAVHLQSNGVTKGLAFL
                     NLAAEFGLDPSDFLAAGDAENDLELLRYAGIGIAVQNAHPAAAAVATYIAQKEFGDGF
                     VEALDRYMPYFLER"
     gene            complement(2369..3340)
                     /gene="radA"
                     /locus_tag="PK154_07475"
     CDS             complement(2369..3340)
                     /gene="radA"
                     /locus_tag="PK154_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HPW10936.1"
                     /translation="MTNGPLELEDLPGVGPTTAEKLREAGFITVESIATASPAELAET
                     AEIGESTAKKMIKSARELAEIGGFRTGKDVFEQRKGVRKLKTRVPELDDLLGGGFETQ
                     AITEMYGEFGSGKSQIVHQMAVNVQLPEEEGGLSGSAIYIDTENTFRPERIEQMVIGL
                     GIDADPAEFLEHIHVARAHTSDHQILMLDTARERAAELRDSDRPVRLFIIDSLTSHFR
                     AEYAGRGTLAARQQKLNRHLHDLFKLADENNAVGLVTNQVLSNPAVFFGDPTKPIGGN
                     IVGHTATFRIYLRKSKGGKRIARLVDSPNLPEGEAPFMVEEAGLRQC"
     gene            complement(3428..4714)
                     /locus_tag="PK154_07480"
     CDS             complement(3428..4714)
                     /locus_tag="PK154_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPW10937.1"
                     /translation="MHFHYALVDDLISREEFERRVEEKMQECGDLIDDVTAAMLVLGE
                     LDRQHVKISGLSGRSTLFSFFGKVITVTPPKEFTRQDGGKGWVAHLILADETGQVRAV
                     LWDEKAGAAAEIETGEVLEVIGKPAGKLKNDITVLALRKSPCIIECGMSVHPQLAPPE
                     RKDIRIRILHLEDPKTITRRDGSTAELIEGCGTDGTAVTRLMTWLPEIFDDILPDTTV
                     FIRGALLKARNWGVEFVIDEKSEVTPDDTCISFSFSTLDTVKNEGDWSVEGSISSLQP
                     VRAFSSRDGRTGHVRNIVLSDGTANLRAVLWGDRALVPLVRGDYLTIYNAQLRQGRSG
                     EPELHVGNGGFVVVTPRGLEEEVEMEGTVIATREGTFLDDGKVRYLVNGTLPHGHEFR
                     VRGIRSGERITISTSEELEKDAVKVRSLLSHLTGAG"
     gene            complement(4732..>5776)
                     /gene="lysA"
                     /locus_tag="PK154_07485"
     CDS             complement(4732..>5776)
                     /gene="lysA"
                     /locus_tag="PK154_07485"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW10938.1"
                     /translation="FSAGELHLALAAGMKPGSLLFNGSSKSPGDLALAVEKGIIVSVD
                     SLDELHQLDAAAGQAGKRAAIAFRVNPALDVPTHPKIATGLATSKFGIAHEMIPAAYD
                     EALGCEYIDPIGIHCHIGSQILSVEPFARACEVMVRIAGVITDMGVRLKFIDLGGGLG
                     IPYHRPDDHAPTPMEYAEAVMPVFRKGIAAAGIAPSLWIEPGRSLVADSTLLLARVNS
                     VKTAHRTFVNVDAGFNLLVRPVMYDSYHEVITANRADQRGTTLCTVAGPICETGDILA
                     SDRFLPDPVAGDILALLDCGAYGFAMSSQYNSRPRCPEILLKGNMAALMRRGETTSDL
                     TCTMQSPPWQKQD"
BASE COUNT         1243 a         1663 c         1541 g         1329 t
ORIGIN      
        1 caccactgag gaaaggcgtg ccaccgtcct cttgcggatc acagagggtg tctctgcctt
       61 ccgtgtctgg tacgcaaggc ctggtccgtg gtttcgtgcc catcgtgcag cccagccgac
      121 tgccccaagt gactttttaa cccggtccat cccccacatg atatcgacaa tatcgcggta
      181 aaaaggcggg agatcgttga gttcaggaaa agacctgatg atctggacga gccggtcatg
      241 gatgccatgg ctcaccgcac gaacgaattc ctcgtttgcc cgatccttgt tcctcttaag
      301 ccgcatcttc gatgcagctc tccggaaact ccggtcgagt atctcatcag cggtcggaac
      361 tgtcggtatt ctctcgaact ccaccggata ttcaacccaa tctatatatg cgaaatcaga
      421 tgataaacca ttatggattt atccctcatc cagaaggata tccttattac cctgatcacc
      481 ctttatcatc agcattcgca ccccatcaag ggggaagaga ttgccgatgt tataaagcgg
      541 aatccgggaa ccgtccgaaa ccagatgcag gcattgaagg ccatcgggct ggtggacggt
      601 attcccggtc cgaagggagg gtataatccc acctcccttg cctacaagga gctgaacctg
      661 ggtatctccg agggagaata tgatgtatca atatcccggg atggtgaggt agtcacggga
      721 gtaaaagtgg tggagattgc attcaccacc ctctgccatc cggatatctg ccaggcaagt
      781 gtccgaatca tcgggagtgt acgtgccttt gagatcgggg acaatatcac gataggaccg
      841 actccggtta acaagctcct gatcaggggg gaggtttacg gaaaggacga aatatcgcag
      901 acccttctca tatccaccta cgagatgatc tcgcttccca aaaaacagat ccggtattat
      961 atgagtgccc ctctcatcac cctcaagggc accgattcga ttgcagatgc catacgtctc
     1021 ttcaccacgc atcacattca cggggctccg gtgatggaga acggcaggct ggccgggatt
     1081 gtcaccatga gtgatattgc caaggcgatc ggcaatcaca agacactcga tgaaaaggta
     1141 gcagcggtca tgaccaccga tgttgtcaag gcaccttcaa acgtgcagct cttcgaagtg
     1201 atccggcgct tcaaggagcg tgagatcggg cggcttgtgg tgattgagga cgatcgcccg
     1261 gtggggatcc tcacccagtc tgacattatc cggcagttcc cgaccctcta gcaagactgt
     1321 atgtatccgg atcttgcctg aatcgactgt ttttcccgaa ttggagccct tattttttaa
     1381 aaatgcaatc cttctggacg aattttgaac atctttaaat attaaaagct caacagttct
     1441 gttatggcca gagtattagc ccgcagccta tccaaaaaga agattgttac caatgaagga
     1501 aaggtgatcg gtaccctgaa aaacctcgtc gttgattttg agaccggaca ggtcgtcgat
     1561 atgatagtgc agcctgaccc ctcatttgat acttcagggt accgggtgga gggggaccgt
     1621 atgtttgtat cctttgaatc ggtcaaggat atcaaggatt atatcatcgt tgaccgctac
     1681 ctctcgagga aatagggcat ataccggtcg agggcttcca caaagccatc accaaattct
     1741 ttttgagcaa tgtaggttgc taccgccgct gctgcgggat gagcattctg tactgcaatt
     1801 ccgatccctg catacctgag aagttcaaga tcgttctcgg catcccctgc tgcaaggaaa
     1861 tcggacggat caagcccgaa ctcagcggca aggttcagga aagcaagccc cttggtcacc
     1921 ccgtttgact ggagatgtac ggcaaaccct gtatccagca catcgacagg aaaatccttc
     1981 acgatctccc tgacctcgtc taccgggaca gtccgggcaa aagcgagatc ggcaaaccgg
     2041 taggtggggc tgtagagatc gagcgtgatt cccgaccggc tataaaaatc ctccaccgcc
     2101 ttgagagcct ctttacagac ggtctggttg ccaaggatcc gcaatggctg ggcataaccg
     2161 gtacggtaca cacctccgtt ctcggcaatg aaggcaccct gtgtaccgat catcttggag
     2221 agtgcgtcca tgaaacagga ggtattaccg cttgccagca cgaccaggat cccttgatca
     2281 cagagcttcc ggatgtattc tatggcaccg gtgtggatcc tccggtgccg gtcggtgagg
     2341 gtgccatcga tatcggtgac gagccccttc agcactgcct cagtcctgcc tcctccacca
     2401 tgaaaggcgc ctcgccttcc ggcaggttcg ggctgtctac cagccgggca atacgcttgc
     2461 ctcccttgct cttccgcagg tagatccgga acgttgcagt atgccccacg atgttcccgc
     2521 cgatgggttt ggtcgggtca ccaaagaaga ctgccgggtt tgagagcacc tggttcgtga
     2581 ccagtccgac agcattgttc tcatctgcaa gcttgaagag gtcgtggaga tgccggttca
     2641 gtttctgctg ccgggctgca agggtcccgc ggccggcata ttcggccctg aagtgtgagg
     2701 tgagcgaatc gatgataaag agcctgaccg gcctgtcgga atccctcagt tcggcagccc
     2761 gttcccgtgc cgtgtccagc atcaggatct gatggtcaga ggtatgggcc cgcgccacat
     2821 ggatatgttc caggaactct gcagggtcgg catcgatacc gaggccgatg accatctgct
     2881 caatcctctc cggccggaag gtattctcgg tatcgatgta gatagcactc ccggaaagcc
     2941 ccccttcctc ctcaggcagc tggacgttga ccgccatctg gtgcacgatc tgactttttc
     3001 ctgaaccaaa ctcaccgtac atctcggtga tagcctgggt ctcgaatcca cccccgagga
     3061 ggtcatcaag ctcgggcacg cgggtcttga gcttccgcac gcccttccgc tgctcaaaca
     3121 catcctttcc ggtccggaat ccaccgattt cagcaagctc ccgtgccgat ttgatcatct
     3181 tttttgccgt ggattctcca atctccgcag tctctgcaag ctcggcaggg gatgcggtgg
     3241 cgatgctctc taccgtgata aagcctgcct cacgcagctt ctctgcggtg gtagggccca
     3301 ctccggggag atcttcaagt tcaaggggtc cgtttgtcat tactcgggtt cacctttttc
     3361 gtggagagca tacatgaatc ctctcatata tcttcgcggc catgcagcgt gaaagtattg
     3421 gttcctttca ccctgccccg gtaaggtggc tgagcagcga tcgcaccttc accgcatcct
     3481 tttcaagttc ttctgatgta ctgatcgtta tccgttcgcc ggaacgtatc cccctcaccc
     3541 tgaactcatg gccgtgtggc agagtgccat tgacaagata ccgcaccttc ccgtcatcga
     3601 ggaatgttcc ctcccttgtg gcaatcaccg tcccttccat ctctacctct tcctcaagcc
     3661 cgcgtggcgt gaccaccaca aatcccccgt taccgacatg cagctcaggt tccccagagc
     3721 ggccctgtct gagctgtgca ttgtatatgg tgagatagtc gccccttaca agaggcacca
     3781 gtgcccggtc tccccagagg actgcccgga gatttgccgt cccgtccgag aggacaatgt
     3841 tccgcacgtg accggtcctg ccatcccgcg aggagaatgc ccggaccggc tgaagggaag
     3901 agatcgaccc ctctaccgac cagtccccct cattttttac ggtatcaagg gtgctgaatg
     3961 agaaggatat gcaggtgtca tcaggtgtca cctcactttt ctcatcgatg acaaactcta
     4021 ctccccagtt cctggctttg aggagcgctc ccctgataaa aacggtggta tcagggagga
     4081 tatcgtcaaa gatctcgggg agccaggtca tcagccgggt aaccgcggtt ccatcggtcc
     4141 cgcatccttc gatcagctcg gctgtgctcc cgtcccgccg ggtaattgtc ttaggatcct
     4201 cgagatggag gatcctgatc ctgatgtctt tccgttcagg gggggcgagc tgtggatgga
     4261 ctgacatacc acattcgatt atgcaggggg acttgcggag ggcaaggacg gtgatatcat
     4321 tcttcagctt tcccgcaggc tttccaatca cctcgaggac ctctccggtc tctatctcag
     4381 ctgcagctcc ggctttttca tcccagagga ctgcccttac ctggcccgtc tcatcagcaa
     4441 ggatcagatg ggcgacccac cctttcccgc catcctgccg ggtgaactct tttggaggtg
     4501 tcacggtgat gaccttgcca aaaaaggaga aaagcgtgga ccgtccggaa agcccgctga
     4561 tcttcacgtg ctgccggtcg agctcgccga gcaccagcat tgcagccgtg acatcatcga
     4621 taagatcgcc acactcctgc atcttctctt ctacccggcg ttcgaactcc tccctgctga
     4681 tcaggtcgtc gaccagggca tagtggaagt gcacggcagg ggctcctctt ttcagtcctg
     4741 tttctgccag ggcggggact gcatggtaca ggtcaggtca ctggtggtct ctccccttcg
     4801 cataagtgct gccatattcc ctttgagcag tatttcgggg catcgcggcc tgctgttgta
     4861 ttgcgatgac atggcaaatc cataggcacc acagtcaagg agtgcgagga tatctccggc
     4921 aaccggatca ggaaggaacc ggtcggaagc aaggatgtcc cctgtttcgc agatcgggcc
     4981 tgccacggtg cagagtgtgg tccctcgctg atctgccctg ttcgctgtga tcacctcatg
     5041 gtacgaatca tacatcaccg ggcggaccag caggttgaaa ccggcatcga cattgacaaa
     5101 tgtccggtgg gctgtcttga ccgagttcac cctcgcaagc aggagggtag aatcagccac
     5161 gagggaccgg cctggttcga tccagagcga gggagctatt cctgctgcgg caatcccttt
     5221 cctgaatacc ggcatcaccg cctctgcgta ctccattgga gttggtgcat ggtcgtccgg
     5281 gcgatggtag gggatgccga gtccgccacc aaggtctatg aatttcagac ggacccccat
     5341 atcggtgatt acccctgcaa tcctcaccat cacctcgcag gctctggcaa acggctccac
     5401 tgagagtatc tgggagccga tgtggcagtg gatcccgatg ggatcgatat actcgcatcc
     5461 aagcgcctca tcgtacgctg cgggaatcat ctcgtgggcg atcccaaact tgctcgtagc
     5521 caggccggtg gcaatctttg ggtgggtcgg cacatcaagg gcaggattga cacggaaggc
     5581 aatcgcagcc cgttttcctg cctgacctgc agcagcatcg agctggtgaa gttcatcgag
     5641 ggaatcgacc gagacgataa tccctttctc aacggcaagc gcaaggtctc cggggctttt
     5701 cgagctcccg ttaaagagga gcgaaccggg tttcatgccg gcagcaagtg ccaggtgcag
     5761 ttctccggcc gagaat
//
LOCUS       DAOOCO010000124         5769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13136_length_5769_cov_4.784914, whole genome shotgun sequence.
ACCESSION   DAOOCO010000124 DAOOCO010000000
VERSION     DAOOCO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13136_length_5769_cov_4.784914"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK154_07490"
     CDS             complement(<1..505)
                     /locus_tag="PK154_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HPW10939.1"
                     /translation="MTAQGIPRIVIAGTHSGCGKTTVARGLMQCLRKRGLIVQPFKVG
                     PDFIDPTYHSSITGRISHNLDPFMMGEDGVRGTFLRACKGADIAIIEGVMGLYDGLDG
                     TDDASTAHVMRILRAPALLVVDVRGMSRSAHALIGGFRSFDRTLDMRGVIFNRLGSPR
                     HRAMIERN"
     gene            738..1205
                     /locus_tag="PK154_07495"
     CDS             738..1205
                     /locus_tag="PK154_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW10940.1"
                     /translation="MGDEDDEIATLHLARWDSRFWAWLIDFILVSLVLNIFSDPFHFF
                     SGILAGDPFDFLVRGTGFLIYWTIMEGYTGQSIGKMVMNLKVTDRSGKPVRFPAAFIE
                     SFGKSFLLPFDCLIGWFGMPGKKLRLFNRVSGSIVIKTDYQEPAGIRYVREHE"
     gene            complement(1448..3880)
                     /locus_tag="PK154_07500"
     CDS             complement(1448..3880)
                     /locus_tag="PK154_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HPW10941.1"
                     /translation="MTEGTEHFAVDTTLDIVQVEYSTSPDCPVIHIFGRDLEKRARHV
                     EITGFRPYFYVLQHQAIAGSIPVNASLEEGKVYRSIRGEPLIRIYTVRPGDVRDIRER
                     FTHFEADIPFTTRFMIDTGLTGGIKIPADSADYREIAPADISSNARICILDIECEDIR
                     GFPEAGRDAVICITCHDSFDDHYTSLIWSGGRDGCDLSVLSDKESLRNGCFSRDRHTI
                     RMFANEKEMFEGFASYLREKDPDILSGWNFLEFDLPYLLKRMEALGMDPASLARLGGQ
                     TERNAIRGRSVFDLLQAYRKMHATQRESYRLDAVAEAEVGETKVRYTGTISDLWKRDP
                     CLLVEYNFKDVELCVAINEKNRIIEFYREIARYVGCPLDRTLNSSSVIDVFVLRKAHG
                     NYVLPSKGFAASEEFEGATVFEPSRGVRENVVVLDLASLYPMAMMTINASPETKDPAG
                     ELKAPNGIRFRKAPDGLTRSILSDLLSEREEKKRERNRYPFGSDEYVIYDIQQNVLKV
                     IMNTYYGVSGYTRFRLYDREIGAAITSVGRAIIEHTRGVIEEMGYHVIYGDTDSCMVL
                     IPPGSVENTIERARGIEREVNASYSRFARQELNADTHFFSIKFEKIYQRFFQAGKKKR
                     YAGRLIWKEGKTVDEIDVVGFEIRRSDYPQITKTVQKRVMEMILHGEQYETIKTYLGE
                     VIKSYRAGRYTLDEIGIPGGIGKDLLDYQTDDAHIRGAKYANEHLGMEFAKGSKPKRV
                     YIRNVKEKYKKTDVLCFEYGDQVPAEFQVDWELMLGKTLQQPISRIIEALGWSWTDID
                     PSRTTLAMWGIG"
     gene            complement(3925..4791)
                     /locus_tag="PK154_07505"
     CDS             complement(3925..4791)
                     /locus_tag="PK154_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HPW10942.1"
                     /translation="MTRLAILGVGRIGGETAYLSTILGLADELVLFDTAKSLLDAQIL
                     DLSHTGIDIGIETDWNQVKDADICVFSAGIPRNPGVRTRADLLSANLPAAKICTQALQ
                     GFSGVLITVTNPMDINNYYLCRSSGLEPRQCIGFGGDLDSSRFGLALRTHGIEGTPWV
                     IGEHGEHQVPLFSRLSTPIQDQLREEILTGLCTASMEVIKGKGGTVFGPAWHLAKLIR
                     IIARDERACISCSCILDGEYGFSQCSLGVPAIVGREGVLSIKEWNLDAWEGEKMKDAG
                     TFVMGLCSYLGI"
     gene            4852..5736
                     /locus_tag="PK154_07510"
     CDS             4852..5736
                     /locus_tag="PK154_07510"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPW10943.1"
                     /translation="MRIVPGEVRIGKVVLSNHLMLAAGILGTTGSSLARILACGAGGV
                     VTKSIGPYPCEGHPGPCVIETEGGLLNAMGLPNPSREFALEVEPLAGEPVILSIYGGN
                     PEEFRMVASWFTGTAAAFELNLSCPHAAGYGSALGTDPALVEACTRAVSSLGIPTWVK
                     LTPNVTDITLCGEAAEQGGASAIVAINTLKAMRISTDLKRPALGNRYGGLSGKAIFPV
                     AVRCVYELYESCRIPIIGCGGISSASDVIEMMMAGASAVQIGTAVKDDPCIFRSIASD
                     LYQSDGVPSDSIVGCAHG"
BASE COUNT         1308 a         1579 c         1505 g         1377 t
ORIGIN      
        1 gattccgctc aatcatcgcg cggtgacggg ggcttcccag gcggttgaag ataacccctc
       61 tcatgtcaag ggtgcgatca aacgatctga atcctccaat gagggcatgc gcgctccgag
      121 acatccctcg cacatccacc accagaaggg caggtgccct gaggatgcgc atcacatggg
      181 cggtgcttgc atcatcagta ccatcaagcc cgtcatataa tcccatgacc ccctcgatga
      241 tggcgatatc ggcacccttg caggccctta aaaatgtccc ccttactccg tcctctccca
      301 tcatgaaagg atcgaggtta tgggagatcc gcccggtgat actggaatga taggtgggat
      361 cgataaaatc aggcccaact ttgaaaggtt gtacaatcag cccccgtttc cggagacact
      421 gcataagccc ccttgcaacg gttgtcttcc cgcatccgct atgggttcca gcaattacaa
      481 tccgagggat gccctgggca gtcattcctg catgatactc cacaattatt ctggtcgtcc
      541 cgggaggtta attccgtttg cccgggataa actctcctcc aattctgaat acgccaaata
      601 tcaggattac tatccacagg aaatgggatc ggcgttatcc cagcaaatcc attcatatcc
      661 gaaggacacc gatatcatga accgaatgag agagttatta aaaaatgcgg ctgatggtac
      721 ctagaggagg acctgaaatg ggagatgaag acgatgagat tgcgaccctc cacctcgcgc
      781 gatgggactc gaggttctgg gcctggctga tcgattttat ccttgtcagc cttgtcctga
      841 atatcttctc cgacccgttc catttcttca gtggaatcct ggcgggggat ccgttcgact
      901 tcctggtacg aggtaccgga tttctcatct actggaccat tatggaaggg tatacaggcc
      961 agtcaatcgg aaagatggtg atgaacctta aggttaccga ccggtcagga aaacccgtca
     1021 ggttccccgc cgcatttatt gagagcttcg ggaaatcctt tctccttccg ttcgattgcc
     1081 tgattggatg gttcggaatg ccagggaaga aactcaggct ctttaacagg gtctccggga
     1141 gtattgtcat caagaccgac taccaggagc cggcgggcat acggtatgtc cgggaacatg
     1201 aataaccaga aggaagggtt ggaaggcatc cctttttttc atcgatgcag agtgggaatc
     1261 gcaaccctct gctgccaaaa tggtttgaca atcccctgac cctctcggaa catgccctga
     1321 catgagggat ctattcagat acgggattag aggtgcggaa caacttttaa aaaggatgca
     1381 tacggtgtcg ggaaaagggc aattctgagg gggcaaccaa cccctcttcc caccgtcaat
     1441 actccgttca cccgataccc cacatcgcga gcgttgtcct ggaaggatcg atatcggtcc
     1501 atgaccaccc cagtgcttcg atgatccggg atatcggctg ctggagtgtc tttccgagca
     1561 tcagctccca gtctacctgg aattctgcag ggacctgatc tccatattca aaacagagca
     1621 catcggtctt cttgtacttt tcctttacat tcctgatata gacccttttt ggcttacttc
     1681 ccttagcaaa ctccatgccc agatgctcgt tggcatattt tgccccacgg atatgggcat
     1741 catcggtctg gtaatcgagc aggtccttac cgatgcctcc cggaatcccg atctcatcaa
     1801 gggtgtacct tccagcgcgg tagctcttga tcacctctcc aagatacgtt ttgatcgttt
     1861 catactgttc gccatgaagg atcatctcca tcacgcgttt ctgaacggtt tttgtgatct
     1921 ggggataatc gctccttcgt atctcgaacc cgaccacatc gatctcatca accgttttgc
     1981 cttccttcca gatgaggcgg cctgcatacc gcttcttctt tccggcctgg aaaaagcgct
     2041 ggtaaatctt ctcgaacttg atggagaaga aatgggtatc cgcgttcagt tcctgcctgg
     2101 cgaaccggga gtagcttgca tttacttcac gttcaatccc ccgcgctctc tcgatggtat
     2161 tttcaacaga tccgggagga ataagcacca tgcaggaatc cgtatcaccg taaatgacat
     2221 ggtaccccat ttcctcaatc acaccgcggg tgtgctcaat gatagccctc cctacagagg
     2281 tgattgccgc accgatctca cggtcgtaca gccggaatcg cgtgtacccg ctcaccccgt
     2341 agtaggtatt catgatcact ttcaggacat tctgctggat gtcatagatg acgtattcat
     2401 cggatccgaa cggatatcgg ttcctctctc ttttcttttc ttcgcgttca ctcagcagat
     2461 cggagaggat actccgtgtc agaccatcgg gggcttttct gaaacggata ccattgggag
     2521 ctttcagctc accggctggg tcctttgtct caggcgatgc gttgatggtc atcatggcca
     2581 tggggtagag agaggcaagg tcgagtacca caacgttctc ccggactccg cgactcggtt
     2641 caaatacggt agccccttca aactcttcgg atgcggcaaa tcctttggag gggagcacat
     2701 agttcccatg cgctttcctc agcacaaaga catcgatgac actggatgag ttgagggtcc
     2761 gatcaagcgg acagccgaca taccgggcta tctcccggta gaactcgatg atccggttct
     2821 tttcattgat ggctacacac agttctacat ccttgaagtt atactcgacg agaaggcagg
     2881 ggtccctctt ccagaggtca ctgatcgtcc cggtataccg caccttcgtc tctcctacct
     2941 ctgcttccgc tacggcatcc agccggtagg attcgcgctg ggttgcatgc atcttccggt
     3001 atgcctggag aagatcaaat accgaccggc cccggatagc attccgctcg gtctggccgc
     3061 cgagccttgc gagggaggca gggtccatcc caagggcttc catccgcttc aagaggtagg
     3121 gcaggtcaaa ttcgagaaaa ttccagcctg acaggatgtc ggggtctttc tcccttaaat
     3181 acgatgcaaa accctcaaac atctccttct cattcgcaaa catcctgatg gtatgccgat
     3241 ccctgctgaa acatccattc ctgagacttt ccttgtctga gagaaccgag agatcacatc
     3301 catctctccc tccactccag atgaggctcg tgtagtgatc atcaaatgaa tcatggcagg
     3361 tgatacagat gaccgcatca cgtcccgctt cagggaaacc acggatatcc tcacactcaa
     3421 tatcgagtat gcagatccgg gcattactgc tgatatctgc cggagcgatc tcccggtaat
     3481 cagcggaatc tgcagggatc ttgatccccc cggtaagccc ggtgtcgatc atgaacctgg
     3541 ttgtgaaggg gatgtcggct tcaaaatggg taaaacgttc acggatatcc cggacatctc
     3601 cgggcctcac ggtatagatc cggataagtg gttctcccct gatggagcga tacaccttcc
     3661 cctcttcaag actggcattc acagggatcg atccggcgat cgcctgatgc tgcagaacgt
     3721 aaaaataagg ccggaatccg gttatctcga catggcgggc tctcttctcc aggtcacgcc
     3781 cgaaaatatg aatgaccggg cagtcggggc tggtcgaata ttcgacctgg acgatgtcca
     3841 gggttgtatc cacagcgaag tgttcggtcc cttccgtcat ggttgctctg ccgggggtat
     3901 agcagtgagg actcataata ttatctaaat cccaagatag ctgcaaaggc ccatgacaaa
     3961 ggtgcctgca tccttcatct tctctccctc ccaggcatca aggttccatt ctttaattga
     4021 tagtactccc tctctcccaa caatcgcggg gaccccgaga gaacactgcg agaatccgta
     4081 ttccccgtca aggatgcagg agcagctgat gcacgctctc tcatcccttg cgatgatccg
     4141 gatgagtttg gcaaggtgcc aggccgggcc gaagaccgtc ccgcctttcc ccttgatcac
     4201 ttccatgctc gccgtacaga gtcctgtgag gatctcctcc ctgagctggt cctgaatcgg
     4261 tgttgagaga cgcgagaaga ggggaacctg atgctcccca tgctcgccaa tcacccaggg
     4321 ggttccctct atcccgtggg tgcggagcgc taaaccaaag cgggaactat cgagatctcc
     4381 gccgaatcca atgcattgcc gcggttccag tccactggat ctgcaaaggt aataattatt
     4441 gatgtccatc ggattggtga cagtaatgag gactcctgaa aaaccctgga gcgcctgggt
     4501 gcatatcttt gcagcaggca ggttcgcgga gagcagatcg gcccgggtcc tgaccccggg
     4561 attgcgtggg attcccgcgg aaaaaacaca gatatctgcg tcctttacct ggttccaatc
     4621 ggtctctatt cctatatcga tcccagtatg ggagaggtcg agtatctggg catcaagaag
     4681 ggattttgcc gtgtcgaaga gcaccagctc atcggcaaga ccgagtatgg tcgagagata
     4741 tgcggtctcc ccgccaatcc tccccacgcc aaggattgcg agcctggtca taatcatcaa
     4801 tattggggag gcgtgaatat aatactcata ctggagattc ggagattgac agtgaggata
     4861 gtaccgggag aggtacggat aggaaaggtg gtgctctcaa accatctcat gcttgccgcg
     4921 gggatactgg gaaccaccgg atcttcgcta gcacgcatac ttgcttgcgg cgctggcgga
     4981 gtggtcacca agtccatcgg gccgtatccc tgtgaggggc accccggtcc gtgcgttatt
     5041 gagactgagg gggggctcct caatgccatg ggcctcccca acccatcacg cgaatttgca
     5101 ctggaggtag agccgcttgc aggggaaccg gtgattctca gcatctatgg aggcaatcct
     5161 gaagagttcc ggatggtagc ttcgtggttt accgggacgg ctgctgcgtt tgagctgaat
     5221 ctgagctgtc cgcatgcagc aggatatgga tctgcattgg gaacggaccc cgctcttgtg
     5281 gaagcatgta cgcgggcagt aagcagcctt gggataccca cctgggtgaa gctcactccg
     5341 aatgtgactg atatcaccct ctgtggggag gcggctgaac agggcggggc ctctgctatt
     5401 gttgcaatca atacgctgaa ggccatgaga atctcaaccg acctgaagcg gccggcactg
     5461 ggaaaccggt atggcgggct ttcaggaaaa gcaatatttc cggtagcggt ccggtgcgtc
     5521 tacgaactct acgagtcatg ccggattccg atcatcgggt gcggcgggat atcatccgca
     5581 agtgatgtga ttgagatgat gatggccggg gcttctgcag tccagattgg aaccgcggta
     5641 aaggatgatc cctgtatttt ccggtcgatt gcctctgatc tctaccagag tgatggggtc
     5701 ccttccgatt ccatagtggg gtgcgcccat gggtgaggaa ccattacagg tggtacagat
     5761 caccacgat
//
LOCUS       DAOOCO010000125         5753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13182_length_5753_cov_5.404352, whole genome shotgun sequence.
ACCESSION   DAOOCO010000125 DAOOCO010000000
VERSION     DAOOCO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13182_length_5753_cov_5.404352"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK154_07515"
     CDS             <1..426
                     /locus_tag="PK154_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HPW10944.1"
                     /translation="RKQALISQITGLDKEIATLEAEQEKLVEAEKRLSTKIEVFRSKK
                     ETIKAQYSAAEAQVKISESVSGISEEMADVGMSIERAESKTEEMKARSAALDELLAEG
                     TLVDFTAGEDDIERELAKIGKQGSIETELAKLKSEVGKK"
     gene            423..701
                     /locus_tag="PK154_07520"
     CDS             423..701
                     /locus_tag="PK154_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10945.1"
                     /translation="MIIRIIGQGQYNVKSSLFDELNKIDNKIVEHVQKGNEKGYKKSL
                     AELIGKIVKEGDVVDHKEIIESDVIVPPSDMTLEEARQVFKGTGIFEG"
     gene            complement(756..1328)
                     /locus_tag="PK154_07525"
     CDS             complement(756..1328)
                     /locus_tag="PK154_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10946.1"
                     /translation="MGIREALGALLGTTALPKANLDKLFAISSASVTMQSEFNLVPAP
                     RAGICFKPIESSQYDEARTEIEELLQFSSRETGSSFRIVTDEYRFTWVILEDPDFDDL
                     VGGIHLVSQTLIEKGFGDYLLCAIFRFEKGKPVYWVYHFKLGRFYPFIPLDGKTRDSA
                     MEFRVRSLLEKDLPIEKEIAKWYPLWGIPL"
     gene            complement(1318..2211)
                     /gene="htpX"
                     /locus_tag="PK154_07530"
     CDS             complement(1318..2211)
                     /gene="htpX"
                     /locus_tag="PK154_07530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPW10947.1"
                     /translation="MVRWAKDRGLQVRMIGTMFLIALVYFIFIAVLAYIGIDSLLLLL
                     IVTGMILLQYFFSDRMVLLSMGARVVTEQEEPRLYETLTRLCSIAGIPRPKLAIVNSA
                     IPNAFATGRNAKNSVVAVTTGLRSTLNQEELEAVLAHELSHVKNRDVMVMTLASLIST
                     AAFFIFRNSLSASRVTSGRGGKQNLWFIIPVFAAAVWVISYLLIKALSRYREFSADRG
                     SAVLTGRPSQLASALMKISGMMDRVPKDDLRKVEGMNAFFIIPALSGSSIMALFATHP
                     SVEQRIAALDRIEKELAGRGD"
     gene            2675..3922
                     /locus_tag="PK154_07535"
     CDS             2675..3922
                     /locus_tag="PK154_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 16 metalloprotein"
                     /protein_id="HPW10948.1"
                     /translation="MKSIDEINHKISLGEAAVLTAAELKRQVADGEHLTVSDVDVVTT
                     ATFGIMSGTLALLHIPVSDPGTFHHAEKAWLNGVPAIPGPCPNERLGLVDLVVFGTSQ
                     ANQSYGGGHLFRDLVEGKEVEVIVETSGRQISRNVTLAEVGLARIVTTRSAYKNYLAL
                     VNRNQGAERTIFSVKGISGPCREVSVSGCGDINPLQNDPQGRVIGVGSRVLLNGGTGY
                     IMGPGTRSTQEHPNIAACADMKRMVPEMMGGFVTSDGPECITSLGAAIPVLDDAILDS
                     ILVTNAGIPLPIADIEGRIVFTSSHYGEVWDHTDLEITYSPEACLQCEPCQAAAVCPT
                     RAIIPGERIDPLHCVHCGTCLQECIGGAFSGNLGTITVDTRQIPITCRQSSREHALRI
                     CDHLKVLVTEGKFNLTGKCGNLW"
     gene            4563..4835
                     /locus_tag="PK154_07540"
     CDS             4563..4835
                     /locus_tag="PK154_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10949.1"
                     /translation="MWYYYLLVLIIGIAFGFLRSGKEDKWGLLKWGLIIGIILGIIFG
                     LISLFTGGILAGLGEAWGVFISIVLYVIVFIIGVFIGDMIEGMKKK"
     gene            5040..5261
                     /locus_tag="PK154_07545"
     CDS             5040..5261
                     /locus_tag="PK154_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPW10950.1"
                     /translation="MPKVNEATVVKVIELIGTSPKSWEDAAANAIKAAGKTVRNITGM
                     ELKRCSATVENSKIVEYKAVVKVAFDVER"
     gene            5571..>5753
                     /locus_tag="PK154_07550"
     CDS             5571..>5753
                     /locus_tag="PK154_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HPW10951.1"
                     /translation="MDIVTAFVGLIQLIIAILLAVVALYIGYSTFSKITKGIDEIQEL
                     KKGNVAVGIIIAAIFFA"
BASE COUNT         1509 a         1402 c         1479 g         1363 t
ORIGIN      
        1 cggaaacaag ccctcatctc ccagataaca ggccttgaca aggagatcgc taccctcgag
       61 gccgagcagg agaaactcgt tgaggctgaa aaacggctct ccacaaagat tgaagtgttc
      121 cgttccaaaa aagagaccat caaggctcag tattctgcgg cagaagcaca ggttaagatc
      181 agtgaatcgg tctccggcat cagtgaagag atggccgatg tagggatgtc gatcgagcgc
      241 gcagaaagta aaacagaaga gatgaaggca cgttcggcag ccctggatga gctcctcgca
      301 gaaggcacgc tcgttgattt cactgctggt gaggatgata tcgagcgtga gctggccaag
      361 atcggcaagc agggcagcat cgaaacagaa ctagccaaac tgaaatcaga ggtgggcaaa
      421 aaatgatcat ccgaatcata ggccaggggc agtataacgt caagagcagt ctcttcgatg
      481 agctgaacaa gattgacaac aagattgtag aacatgtcca gaaagggaat gaaaaaggat
      541 acaagaaaag cctcgccgag ctgatcggaa agatagtcaa ggagggggat gtggtcgacc
      601 acaaggagat catcgaatcc gatgtcatcg ttcccccatc tgacatgacg ctggaagagg
      661 cccggcaggt cttcaagggc acggggatct ttgaagggta gccggaacgt cccccaatca
      721 caatgttttg aacaatacta aaaataacag aacatttaca gcggaatacc ccacaggggg
      781 taccacttcg ctatctcttt ttcgatggga agatcttttt caagcagcga ccggaccctg
      841 aactccatgg cactgtcccg ggttttgccg tcaaggggaa tgaatggata aaatcgtccc
      901 agcttgaaat ggtataccca gtagactggt tttcctttct cgaaccggaa gattgcacag
      961 agaaggtaat caccgaatcc cttctcgatc aacgtctggc ttaccaggtg aatcccccca
     1021 acaagatcat cgaagtcggg atcttccagg atcacccagg taaaccggta ttcatcggtg
     1081 actattctga aagagcttcc cgtctcccgg gagctgaact ggagcagttc ctcgatctcc
     1141 gtccttgcct cgtcgtattg ggaggactcg atgggtttaa aacatatgcc ggctcgaggg
     1201 gcaggaacaa ggttaaattc gctctgcatc gtcacgctgg ctgaagagat ggcgaacagt
     1261 ttgtcgaggt ttgccttagg gagagccgtg gttcccagca atgctcccag tgcttcccta
     1321 atccccacgg ccggccagct ccttttcaat ccgatcaaga gcagcaatcc gctgctcaac
     1381 ggacggatgt gtcgcgaaca gcgccatgat cgatgatccc gagagagcag ggatgatgaa
     1441 gaaggcattc atcccttcca cctttcgcag atcgtctttt ggtacccggt ccatcatccc
     1501 actgatcttc atcagggccg atgcaagctg ggacggccgt ccagtcagga ctgcagagcc
     1561 acggtcggcg gagaactcgc gataccgcga gagcgctttg atcaggaggt agctgatgac
     1621 ccagacggcc gctgcgaaca ccgggatgat gaaccagaga ttctgcttcc ctccccttcc
     1681 cgatgtgacc cttgaggccg acagggaatt tctgaagatg aagaatgctg cagtagagat
     1741 caggcttgca agagtcatca ccatgacatc ccggttcttc acatggctca gttcatgggc
     1801 aaggaccgct tcaagttctt cctggttgag cgttgagcga agcccggtgg taaccgcaac
     1861 aacagaattt ttggcatttc tccccgttgc aaacgcgttt ggaatggcac tgttcacgat
     1921 cgcgagcttc ggacggggga tcccggcaat cgagcagagc cgggtaagag tctcgtagag
     1981 tcgcggctct tcctgttcgg tgacaacgcg ggcacccatg gagaggagga ccatgcggtc
     2041 cgaaaaaaaa tactggagga ggatcatccc tgtcacaatg aggagcagca ggagagaatc
     2101 aatcccgata tatgccagca ccgcgatgaa gataaaatac accagggcta tcaggaacat
     2161 tgttccgatc attcgcacct gcaatcccct gtcctttgcc caccggacca taacgataga
     2221 tctccctgat tttcaatgcc agcagaaatt cccctccaga ataatgaaca ggtaacggct
     2281 gagaattact gcagttctag gcatgtatca catagactat cggacgataa atagattctc
     2341 actccggaac agggttcttc ttcccaaaaa aatccaataa aaatcgtcca gacatgcgct
     2401 ctgaagaagt tctgatgatg ctactctgtt taggtgtgaa gcaatgaaac gtcctgccaa
     2461 atcctggagg ctgtttcaga tgcgttcata acaattcaga tgtggtagct ttgagagaga
     2521 tcccacattc cggtcaggca gtaacaataa tcccccaggg gcacaatcat ttgaaacggg
     2581 gggaaggaac cgatacaacc tcaccaattc catgagaatg aaaaagaata cccgttctga
     2641 cagagactca ctttgagaga ggtatcccgg caccatgaag tcaatcgatg aaatcaacca
     2701 taagatatca ctcggggagg ctgcggttct caccgctgct gaactcaagc gacaggtcgc
     2761 tgacggagag cacctcaccg tgtcagacgt ggatgtggtc actaccgcga cgtttggtat
     2821 catgtcagga acactcgccc tgttgcacat cccggtctct gatcccggta cctttcacca
     2881 tgcagaaaag gcctggctca acggggttcc cgctataccc ggtccctgtc cgaacgaacg
     2941 gctcggtctt gtcgaccttg tggtctttgg aacgtcccag gcaaaccagt catatggcgg
     3001 gggccatctc ttccgggatc tggtagaggg gaaggaagtg gaagtcatcg ttgagaccag
     3061 cggacgtcag atctcccgga atgtgactct cgctgaggta ggtcttgccc ggattgtaac
     3121 cacacggagc gcatataaga actacctggc acttgtcaac cggaaccagg gtgcggaaag
     3181 gaccatcttc tcggtcaagg ggatttcagg gccgtgccgc gaggttagcg taagcggatg
     3241 tggagatatc aacccgctcc agaacgatcc acaagggagg gtgataggtg ttggcagccg
     3301 ggttctcctc aatggcggga cggggtatat catgggaccg gggaccagaa gtacacagga
     3361 acaccccaac attgctgcat gtgctgacat gaaaaggatg gtcccagaga tgatgggagg
     3421 atttgtcacc tccgatggac ctgaatgcat cacctccctt ggcgccgcaa tcccggtgct
     3481 tgatgacgct attctcgata gtatccttgt caccaatgca ggcattccgc tgcccatcgc
     3541 tgatatagag gggcgtattg tcttcacctc gtcacattac ggtgaagtat gggatcatac
     3601 tgaccttgaa atcacctatt ccccggaagc ctgcctccaa tgcgaaccct gccaggctgc
     3661 tgcggtctgc cctacccggg caattattcc cggagagaga atagatcccc tccactgtgt
     3721 ccattgtggc acctgtctcc aggaatgtat tggaggagcg ttctccggca acctcgggac
     3781 cattaccgtg gataccaggc agatccccat cacatgcagg cagtcatccc gggagcatgc
     3841 cctccggatc tgcgaccatc tcaaggtgct cgtcaccgaa gggaaattca acctcacggg
     3901 aaaatgcggg aatctctggt gaaggaaatt cccaaggcga tccacttgca ccgagagtaa
     3961 atcagcactg gttcaaaaaa gagagggtga atttcaggat taatgagtga cgagttttgg
     4021 taaactcttt gcccggtcca cccagccctc gaccattttc tgggtaggaa cccgccggac
     4081 caggttcttt gcagcattgg cctcttcaag cttctttacc agttgtccgg tttccggata
     4141 gccagttcag gtacactgtc ctccccggca gcttcgagga gatccgaata ctctgatccg
     4201 atacccttta tccggtacag atcagcgggg ttgacctacg tgagaatctt tgagggatca
     4261 ataccggtag cttcggcaat ctcctcatcc ccttttcaga agcacctttt tcaaggagtc
     4321 cttcaaccgt cttgatcccg gttttttcga atttggcttt gattccgggt ccaatacctt
     4381 caatgtcgga taatttcatg attaaatttg ttacgctcac tttgtaaaaa tggttttaat
     4441 tatccccttt ttggcaatta tttctattta acgagatgga gctgattttc ccctgcatac
     4501 ctgattgagc catactttta tcataaagaa agaggatatt gggactgaaa cggagaattt
     4561 atatgtggta ctattattta ctggtcttaa ttatcggtat agcctttggg ttcctgcgga
     4621 gtggaaaaga agataagtgg ggtctcctca agtggggatt gatcattggg attattcttg
     4681 ggatcatttt cggcctcatc tctttgttca ctggaggtat cctcgctggt ttgggtgaag
     4741 catggggagt attcatctcg attgtgctct acgtaatagt tttcattata ggtgtcttca
     4801 ttggcgacat gatcgaggga atgaagaaaa agtagagggg atagccctga atatggtgtc
     4861 tctatcaatc gttccatctt tttttcccat ggaatagttc tcttttcaga ttactatgga
     4921 gatactctta tccatccatg agtaaacgcc tttttctgtg agaaagtaat ccaaatttca
     4981 gagcgagagc gtaaacctca tgtagaaggg ataccgatcg ttgaaatgga gcaagagaaa
     5041 tgccaaaggt aaatgaagcc acggtagtaa aagtcatcga actgatcggc acctccccaa
     5101 agagttggga agatgcggca gccaatgcga taaaggcagc gggaaaaacg gtgagaaata
     5161 tcacgggaat ggagttgaaa cgctgctctg ccaccgttga aaacagcaaa attgttgagt
     5221 ataaggctgt tgtgaaggtt gctttcgacg tcgaacgata aatccggaaa cgcaatcctt
     5281 cgaggggagg cgccaaccac ttcccttccc taatgttcag gcacaacctt tcatgcaaac
     5341 gtgatcctct tctcagggag gggaagttcc atatctctcg aactgaatcg gttgttcaga
     5401 atcagtgccc tgcttgtttc acgaaaattc ggggaggaga gagaaagaag cgcccatata
     5461 cctttatttg agattcggtc ttattttatt gcctggaaaa tcactcttct ggggaaatgt
     5521 ttattttggg tagttctata ccattgtaac cataaagagg tgaagaaagt atggatattg
     5581 ttaccgcctt tgtcgggctg atccagctga tcatagcgat ccttctggca gtagtcgctc
     5641 tttacattgg ttattcaaca ttctcaaaga tcacaaaagg aatcgatgaa atccaggaac
     5701 tcaagaaagg aaatgttgcg gtgggaatca tcattgcagc gatcttcttt gct
//
LOCUS       DAOOCO010000126         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13251_length_5732_cov_5.089660, whole genome shotgun sequence.
ACCESSION   DAOOCO010000126 DAOOCO010000000
VERSION     DAOOCO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13251_length_5732_cov_5.089660"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..720)
                     /locus_tag="PK154_07555"
     CDS             complement(349..720)
                     /locus_tag="PK154_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPW10952.1"
                     /translation="MHGSGANGNRRVAVAVDGNGVSSHFGHCEKFSVYHIRNKEIFHH
                     EIILNPGHEPGKLPRLLASCGVTNVLAGGMGMKAVQLFEEQGIHVMCGISGPVDQVIR
                     DFIDDRIVAGASTCHHSGCDE"
     gene            complement(724..996)
                     /locus_tag="PK154_07560"
     CDS             complement(724..996)
                     /locus_tag="PK154_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPW10953.1"
                     /translation="MPGLDGTGPRGGGPMTGWRRGRCNPVSQKESAMDSVEEAGQATV
                     QQTGGAPSPVYGLGRGGIPCGCGRGYGGGMRGGRGGPHGNGMRGRF"
     gene            complement(1088..1477)
                     /locus_tag="PK154_07565"
     CDS             complement(1088..1477)
                     /locus_tag="PK154_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPW10954.1"
                     /translation="MRRRGRPRVPRRMDHEASLRCYAPQCDIPDNDSVILLPEELELL
                     KLVDLNGLEQEEAGAIIGVSRRTVWKDLHEARRKVADALIHGKKIEIAGCERRIEGQC
                     PREGQPCLGSQDGFCRRRNRRDGSPDQ"
     gene            complement(1491..2399)
                     /locus_tag="PK154_07570"
     CDS             complement(1491..2399)
                     /locus_tag="PK154_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW10955.1"
                     /translation="MQVIGGGNMIRLAVVSGKGGTGKTVITGGIVHLSRCAKVMVDCD
                     VDAANLELIVNPHIIKREEFTGMEGAYINPAQCSSCGVCSDACRFGAIEPIGDTYAVH
                     PDQCEGCGVCRVICPSMAISMRPRVCGEIYYSETPYGNLVHARLAPGAANSGLLVGKI
                     KKIAVERNGECDLMLVDGPPGIGCPLISTITGMDQLLVVTEPSVSGLHDLQRLIRVCR
                     PLRVNMMVAINKYDLNLGVSNSIREYCGKEDIPIGGMIPYDESVIDVVRKGRLVTEYD
                     TPAAQAIHSLWDEIVSTLGVRDIAGQ"
     gene            complement(2372..3235)
                     /locus_tag="PK154_07575"
     CDS             complement(2372..3235)
                     /locus_tag="PK154_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW10956.1"
                     /translation="MTTSMKITIASGKGGTGKSMVAANLAWAISQSDRVTLVDCDVEE
                     PNLHLFFPPDDPESTDVQVLIPAFDNSRCTLCGDCAGFCAFGALVVAKDRVLFFPEHC
                     HSCGGCALVCPADAITEIQRPIGTVTTRDLSAHLSLVTGTLNEGEVLGVKVIQEAKRR
                     AEKPGWMICDASPGTSCPVVETLHGSDFCLLITESTPFGLHDLELAYSVTSHLDVPVG
                     VVINRSDGKDEEVRRFCNERGIPVLATIPFDRWIASVQGSGDLISRVDPTWKERFIAL
                     GGACRSSVGGT"
     gene            complement(3274..3609)
                     /locus_tag="PK154_07580"
     CDS             complement(3274..3609)
                     /locus_tag="PK154_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPW10957.1"
                     /translation="MKICIPSRGPDIQDLVDERFGRAPYFIIHDTESKMTEAHVNSAA
                     GAMGGVGPKAAQFLLNQGVDSLVTERVGGNALEALKAGGIMVLLYEEKGSTVADAITA
                     FLKGNLKEQ"
     gene            3850..5484
                     /gene="atwA"
                     /locus_tag="PK154_07585"
     CDS             3850..5484
                     /gene="atwA"
                     /locus_tag="PK154_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HPW10958.1"
                     /translation="MSGSPSPLLTVRNLSIELGGTLILDNVSFHMNEGEVLGIIGRSG
                     AGKSILMHLMRGIDDPPTSGAVIYHMSQCQKRCSLEPRSAEGMPCPGCGGALLPVDID
                     LWNPDDERGKSLILRKTAIMFQRTFALYGNDRVIENVLHALDDIRYPPDQSVNRAAEL
                     LERVRMTHRMMHIARDLSGGEKQRVVLARQLAKEPLLLFADEPTGTLDPTTAHLVHQL
                     LLDATSNSHMGMVITSHFSGVIDQVADRALLMDKGRVIEEGSPTKILGKFMQNYHAGE
                     RVDSSENGAPILQAREVMKRYITADRGLIKAVTNVTFDVAEREIFGIIGTSGAGKTTL
                     SRIIAGLIEPTGGEMNIRIGDDWVDMTSPGIENRGRAKPYIGLLHQEYDLYPHRTILD
                     NLTDAIGLEFPKELAIQKAVISLKMAGFAPDDITTFLHRYPAELSQGERHRVALAQVL
                     IREPRIVVLDEPTGTMDPITKLDVKHSILTARKEMDETFIIVSHDMDFVQDICDRVAL
                     MKGGKVIHIGPPSEIPMEFIIPASDREASQDGIREP"
BASE COUNT         1385 a         1641 c         1352 g         1354 t
ORIGIN      
        1 cgagttcatg cccgccggag agcgctcact gatcatcccc gcaaggtgag atcagcccca
       61 atttttttcc ttttctgaca atccagattc ttacagcgac atacgccact gcccgcccgg
      121 agatggcatt ggaattacgg aaagggattt ttcaatctta tcaggacagt gcgtatcagg
      181 acgatggagg aacctcaggt gatttcggtt gaaggaacga aacgctcttt gaaaagaacg
      241 cggatgttcc atgaggtacc cccctgaatc cagagtccaa taagcgtgta ggggagactg
      301 aaacagatac gttttagaaa cgaatcctac tgataaaagt tggtttgcct actcatcaca
      361 gcctgaatga tggcacgtac ttgcaccggc aacaatccgg tcatcgataa aatccctgat
      421 gacctggtca accgggcctg aaattccaca cataacatgg attccctgct cttcgaagag
      481 ctggaccgcc ttcatcccca ttcccccagc aagtacattg gtaaccccac aggaagcaag
      541 gagccgggga agttttcctg gttcgtggcc gggattgaga attatctcgt ggtgaaaaat
      601 ttctttgttc ctgatgtgat acactgagaa tttttcacaa tgaccaaagt gtgaggagac
      661 accgtttcca tctaccgcga ccgcaaccct ccggttgcca ttcgctcccg atccatgcat
      721 tcttcagaat ctccccctca ttccattccc atgggggcca cctctgcctc ccctcatacc
      781 cccgccgtat cctctcccgc atccacaggg gatacccccg cgaccgaggc cgtataccgg
      841 agagggcgct cctcccgtct gttggacagt cgcctgccct gcctcttcga ctgaatccat
      901 tgcagattcc ttctgtgata ccgggttaca cctgccacgg cgccacccgg tcatcggtcc
      961 ccccccacgc gggccggtcc cgtcaagtcc tggcattttc ttatcacacc tgggcaccgg
     1021 tcgggtgcct taattactca tatgagtcaa aatatattaa aataatgtga tgaggcagac
     1081 ctgtctctta ctgatccggg gaaccatccc gcctattccg gcgcctgcaa aacccatcct
     1141 gcgaacccag gcagggctgc ccttcacggg gacattgccc ctcgatacgg cgttcacatc
     1201 cggcgatctc tatttttttc ccatggataa gggcgtcagc aacctttcgc cttgcttcat
     1261 ggagatcttt ccagaccgta cgccgcgaca ccccgatgat agcacccgcc tcctcctgct
     1321 ccagcccgtt caggtcgacc agtttcaaca gttcgagctc ttcaggaagg aggataacag
     1381 agtcattatc gggaatatca cactggggcg cataacatcg gagcgatgcc tcgtgatcca
     1441 tccttctcgg tactctcggt cgcccccggc gtcgtattcc tccagtcgtc tcattgtcct
     1501 gcaatgtccc tcactcccag tgtactgaca atttcatccc aaagactatg aatggcttgc
     1561 gctgcaggag tatcatattc ggtaaccagc cttcctttcc gtaccacgtc tatcaccgat
     1621 tcatcataag gtatcattcc ccctatcgga atgtcctctt ttccgcaata ctcgcggatg
     1681 gagttggata caccgaggtt gagatcgtac ttgttgattg cgaccatcat attcacccga
     1741 agcggacgac aaacacggat gagccgctgc aggtcatgaa gtcctgaaac actcggttca
     1801 gttaccacaa ggagctgatc catccccgta atggttgata tcaggggaca gccgattccc
     1861 gggggaccat caacgagcat gaggtcacat tcaccattcc gctccaccgc aattttctta
     1921 atttttccga ccaggagccc cgagttcgcc gctcctgggg ccagcctcgc atgtaccagg
     1981 tttccatagg gggtttccga ataatatatc tctccacata ccctcggccg catgctgatc
     2041 gccatggaag ggcagataac ccggcagacc ccgcagcctt cacactgatc agggtgtacc
     2101 gcataggtat ccccaatagg ttcaatcgct ccgaaccggc aggcatcaga gcaaacaccg
     2161 cagcttgaac actgggcagg gttgatatat gccccctcca ttccggtaaa ttcctcgcgc
     2221 ttgatgatat gaggattcac aatcaactca agattcgcgg catcaacatc acaatcaacc
     2281 atcacctttg cacatctgct gaggtgaacg ataccccctg taatcaccgt ttttcctgta
     2341 cctcctttcc cactgaccac cgcaagccgg atcatgttcc ccccaccgat gacctgcatg
     2401 ctccaccaag agcaatgaac cgctccttcc aggtgggatc aacacgggat atcagatcgc
     2461 cgctcccctg gactgatgca atccatcggt caaatggtat ggtggcaagc accggtatcc
     2521 cccgttcatt gcagaacctc cgcacttcct catccttccc atcgctccgg ttgataacta
     2581 cccccacggg tacatccaga tgggaggtca cggaatatgc cagttccaaa tcgtgaagtc
     2641 cgaacggtgt cgactcagtg atgagaaggc agaaatcgga tccatgcaga gtctcaacga
     2701 ccgggcagga tgtaccaggt gacgcatcac agatcatcca cccgggcttt tctgcacgcc
     2761 tctttgcctc ttggatcacc ttcactccga gcacctcgcc ctcgttgagt gtccccgtga
     2821 caagggacag gtgagccgag agatcccggg ttgttactgt gccaataggg cgttgaatct
     2881 ctgtgattgc atcagccgga cagacaaggg cgcacccccc gcatgagtgg cagtgttccg
     2941 ggaagaatag tacccggtct tttgccacga ccagggctcc gaacgcacag aaccctgcac
     3001 aatccccgca taaagtacat cgcgaattat caaaggcggg aattaatacc tgcacatcgg
     3061 tcgattcagg atcgtctggg gggaagaaaa ggtgaagatt tggttcttcg acatcacaat
     3121 cgaccagtgt cacccgatcc gattgggaga tggcccacgc caggtttgca gccaccatgc
     3181 tctttccggt tcctcccttc ccgcttgcga tggtaatttt catactcgtt gtcatcttct
     3241 cctgcctcat cggaagaacg aataaaagaa gaattattgt tccttcaggt tccctttgag
     3301 aaaggccgtg atcgcatcgg caacggtgct tcctttctct tcgtaaagaa gcaccatgat
     3361 tcctcccgcc ttcaatgcct caagggcatt tccaccaacg cgttccgtca caaggctgtc
     3421 tactccctgg ttgaggagga actgagcagc ctttgggcca acgcccccca tggcacctgc
     3481 ggctgaattt acatgggctt cggtcatttt gctctcggtg tcgtggataa taaaataggg
     3541 agcacgtcca aaccgttcat caaccaaatc ctggatatcc ggcccccttg agggaataca
     3601 aattttcatg gatttcacca ccttaattta ttcacatatg tgaagaaatt atttttcata
     3661 tcggcattcg gttctctgct tcagtccctt ggtcgatgcc cattcttctg tcctattaaa
     3721 tacctttaat tattttttac tcatatgtgt agttatagtg tggcagcagc ccaaccccct
     3781 gcactattat tccggcaggg tccagccctt cgatccggaa aaacaagtga ctgaatggat
     3841 gagtagccta tgtctggttc accctcaccc ctgttaaccg tcagaaacct cagtatagag
     3901 cttggcggaa ccctgattct tgacaatgta tcctttcaca tgaatgaagg ggaggtcctc
     3961 gggatcatcg gaagaagcgg cgccggaaaa tcgatcctca tgcacctgat gaggggtatc
     4021 gatgatccgc caacatcggg tgctgtcatt taccacatga gccaatgtca aaagcgatgt
     4081 tcactcgaac cccggagtgc tgaaggaatg ccctgccccg gttgcggagg agcgctcctc
     4141 cccgttgata tcgatctctg gaatccggat gatgagagag gaaagtccct gatattgcgt
     4201 aaaaccgcca ttatgttcca gcgtactttt gcattgtatg ggaatgacag agtgatagag
     4261 aatgtccttc atgcacttga cgatatcagg tatccacccg atcagtctgt gaaccgtgcg
     4321 gcagaactcc tggaacgggt acggatgacc catcgtatga tgcacatcgc acgcgatctc
     4381 tccggtggtg aaaagcaacg ggtggttctt gcgaggcagc ttgcaaagga gcccctgctc
     4441 ctctttgcag atgaaccaac cgggacctta gatcccacca ctgcccatct cgtccaccag
     4501 ctgctccttg atgcgaccag caactcacac atgggaatgg tcatcacctc acatttttca
     4561 ggggtcatcg accaggtggc tgaccgtgca ctgcttatgg ataaagggcg ggtgatcgag
     4621 gaaggctcac cgaccaagat cctcggaaaa ttcatgcaga attaccatgc gggagaacgg
     4681 gtggattcca gtgaaaacgg ggcgccgatc ctccaggcaa gggaggtcat gaaacggtac
     4741 attaccgctg accggggact gatcaaggca gtcacgaatg tcacatttga cgtcgccgaa
     4801 cgtgaaattt ttgggatcat cgggacgagt ggtgccggga agaccactct ctcgcgtatc
     4861 atcgccgggc tcattgaacc cactgggggg gagatgaaca tccggatcgg tgatgactgg
     4921 gtagacatga cgagcccggg gatagagaac cgggggcgtg caaaacccta catcggcctc
     4981 ctccaccagg aatatgatct ctatccccac cgtaccattc tcgataatct caccgatgca
     5041 atcggtctcg aattcccgaa agagctcgcc atacaaaaag cagtgatcag cttgaaaatg
     5101 gcaggatttg cacccgatga tatcactacc ttcctgcacc ggtatccggc cgagctgagc
     5161 cagggggaac gtcaccgcgt cgctcttgcc caggttctca tccgggagcc aagaattgtt
     5221 gttctggatg aaccgaccgg gaccatggac ccgatcacta aacttgacgt aaagcattcg
     5281 atcctgactg cacgcaagga gatggacgag acgtttatca tcgtctccca tgacatggac
     5341 tttgtccagg atatctgtga ccgggtagcc ctgatgaaag gagggaaggt tatccacatt
     5401 ggtccgcctt ctgaaattcc catggaattt atcatacctg cttccgaccg ggaggcatcc
     5461 caggacggga tacgtgagcc gtgagaaaag ccctgaagat actcccccta taccagttaa
     5521 tacaaataat cttgcaaaca acacaacctt tttttaatta agtaaatttc agatgcgtta
     5581 atttcgcagg aaaatcctcc gttttgtgat taaaacccaa aaaaccggtt tataagcaaa
     5641 tatttcgaaa catttaactc attactgacg taactgttag atgaaccaca aagggtttgt
     5701 actcgttgtg agtggcaatg catgatgaac ac
//
LOCUS       DAOOCO010000127         5718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13291_length_5718_cov_5.293661, whole genome shotgun sequence.
ACCESSION   DAOOCO010000127 DAOOCO010000000
VERSION     DAOOCO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13291_length_5718_cov_5.293661"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PK154_07590"
     CDS             complement(<1..769)
                     /locus_tag="PK154_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10959.1"
                     /translation="MDAKFAVGAVLLIGVILIAGCTQQPQAEQTLKIGVVASMTGSAS
                     TTGKDIWQSAVLAVEEINADGGVYIKDLGRKVPIEAILGDDESTREGGQKAVTRLVTE
                     DKVDLLVGGFSSAVVSSHQAIVNEHQVPYIVTGASSPIITQRTDMPTSTTFHYCPTTD
                     DYGRQTTLFANEVIRPAINEKFGFSSGRPLRMALIYQDSPYGKGVQTAVINTIEQEHL
                     PITIVANETFKMGETDYRTLLTSVKAAQPDVIYPATFI"
     gene            complement(844..1794)
                     /locus_tag="PK154_07595"
     CDS             complement(844..1794)
                     /locus_tag="PK154_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW10960.1"
                     /translation="MRLKDALPKMPKIGTTHYLTFIVFLGILNASIGLAYVEAKTSEI
                     TVLHIDGAPDNIVFIADPHLKQGNINYVNTIINEINALDPSIVMIGGDFVYGDGEDLS
                     LQKIWEGIDAPVYAVLGNHDYKSGITSTSWVEKNLAVNHASFDRDDYNVSSLRDETTD
                     VVYAGRLTDELGMNGVRVLKNEYLVVDADGQELILVGIDDGWAGMADPPDVPKTCAFT
                     LYLIHEPECRANWDADLILAGHTHGGQFLPQDIPMPGFELSGLSLENGVSTYITRGIG
                     TSNLGVELRLFATPEIVVINPTCSPEELFPDKKITHITLP"
     gene            complement(1893..1977)
                     /locus_tag="PK154_07600"
     tRNA            complement(1893..1977)
                     /locus_tag="PK154_07600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1941..1943),aa:Ser,seq:tga)
     gene            complement(2018..3151)
                     /locus_tag="PK154_07605"
     CDS             complement(2018..3151)
                     /locus_tag="PK154_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10961.1"
                     /translation="MDQKRPASHTLARIALVALLCLALCGGVAQAYELILKAPSQIQR
                     GMPLVVNGTSNLPPGISVDIVLYKSGHVTEELARKTVTLQANNEFSVVFDTTGFTKGV
                     YKAEVPAISGYSYLGDSVTLRVIEIIDRSDEIVFSAFSSQEMDGTLDIDGSIAGLANS
                     GVQIGVTGPGGEAVFGPAYVSVKSDSSFSTEVPITGPGQYNISFTDAKGYIGTIMVTV
                     REKPVATTPPTIVPTTNPILSASAFASRDKPATFVVTTGGPGTIRAFTSSGIDWVIEY
                     TDTEGKTVKVNEKGSVGNEEIIIDTAEDVLVLKVYPYSYAAEGDVLLSADGAVKVETS
                     GPGTAATTPGTPSTEEKSSPLPAIGVVAAVIAGVALVVIRRNW"
     gene            3310..4275
                     /locus_tag="PK154_07610"
     CDS             3310..4275
                     /locus_tag="PK154_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HPW10962.1"
                     /translation="MERPLACWKGEERYEGEVLPSLTIIMKTGGCRHNRCRMCSYRFE
                     RYPEGTDLEGALLSQLHWVVQHYPPSGVAIVKLYTSGSFFDPGEVPPRVVEEAARIFW
                     GKLVIAETRPEFVDPERIAAFISAIDSGDYPTPLYVAIGLETLNDSIRQKSIDKGFTF
                     SDFKKAADNARAGGAGIKAYLLLKPLFLTEREALLDMEESIRTVTGIADMISMNPCTV
                     QKHTELEYYWKRGEYRPPYLWSVVSLLLSAPAHITCDPLGGGRARGPHNCGTCDYDIV
                     KAIRDYNLSADCQTLHQLFSGGCECKEEWERVLSLEKPYCMPLTR"
     gene            complement(4237..5502)
                     /locus_tag="PK154_07615"
     CDS             complement(4237..5502)
                     /locus_tag="PK154_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HPW10963.1"
                     /translation="MQVSREVELEGHIIDSGIMTKVFDRIMDKGGNFEIITFEVGKRK
                     TDPSYARLKVNTDTEQQLQAILSEIHRFGAHLLFEEEISVAPAEGDRIVPKGFYSTTH
                     HPTSVKYRGRWILVEHIEMDCTIVVDPEGKSALCTPLSKLKKGDLVVVGEEGVRVDPP
                     ERPRQLSNFEFMHGTVSPERPSETIIAKVAAEMIALKAKGGKIGLVGGPAIIHTGAAR
                     ALASIIRKGYIDVLFAGNALATHDIEYNLYGTSLGMDICTGKPVTGGHKNHLYAISEV
                     MRAGSIKKAIETGVITGGIMYECVMKDIPFVLAGSIRDDGPLPDVITDAVVAQDQIRS
                     HVEGCGMVLMVATLLHSVAVGNCLPSFVKTICVDINPSSLTKLMDRGTMQAIGVVSDA
                     GTFLPLLEKQLEIQEVPSPSQRHAVRFLE"
BASE COUNT         1240 a         1565 c         1577 g         1336 t
ORIGIN      
        1 tgatgaacgt cgccgggtag ataacatcag gttgtgccgc cttgaccgag gtgagcaggg
       61 tccggtaatc ggtctcgccc atcttgaagg tctcgtttgc tacgatcgtg atgggaagat
      121 gctcctgttc gatggtgttt atcaccgcgg tctgcactcc cttcccatag gggctgtcct
      181 ggtagatgag cgccattctg agcggccttc cggatgagaa gccaaatttt tcgttgatgg
      241 cgggacggat cacctcatta gcaaagagag tggtctggcg cccgtagtca tccgttgtcg
      301 gacagtaatg gaacgtggta ctggtcggca tgtcagtccg ctgcgtgatg atcgggctcg
      361 atgccccggt aacaatatag ggaacctggt gctcgtttac aatcgcctgg tgcgatgaga
      421 caaccgcact cgagaatccc ccgacaagga gatcgacctt gtcctcggta accaaccggg
      481 ttaccgcctt ctgcccccct tcacgggtcg attcatcgtc accgaggatt gcctctatgg
      541 ggactttcct gccgaggtct ttgatgtaca ccccgccgtc cgcattgatc tcctcgacag
      601 caagcacggc cgactgccag atatcttttc cggtggtgct agccgatccg gtcattgagg
      661 caacaacccc gatcttgagg gtctgttcag cctgaggctg ctgagtacat cctgcgatga
      721 gaatgacgcc gatcagcagc actgccccta cagcaaactt cgcgtccaat ccaaacaccc
      781 tgctatgtta aagcatggca tggcagctcc tataaaattt ccccttggaa aaggcaggac
      841 ggatcagggg agcgttatat gggtaatttt tttatcgggg aataattctt caggggaaca
      901 ggttggattg atgacgacta tctccggagt ggcaaagagc ctcagttcga cgccgaggtt
      961 agaggtccct ataccccggg taatgtaagt actcacgccg ttctcaagag agagcccgga
     1021 gagctcgaat cccggcatgg gaatgtcctg ggggaggaac tgccccccat gggtatggcc
     1081 ggcgaggatg agatcagcat cccagttggc ccggcattca ggctcatgga tcaggtagag
     1141 agtgaatgca caggttttcg ggacatcggg aggatccgcc attcctgccc agccgtcatc
     1201 aataccgaca aggatcagct cctgtccgtc tgcatccacg acaaggtact catttttgag
     1261 tactctcaca ccgttcatcc cgagctcgtc agtgagccgg ccggcataga caacatccgt
     1321 tgtctcatcc cggaggctgc tcacattgta atcgtccctg tcgaacgagg catgattcac
     1381 cgcgaggttc ttttctaccc aggatgttga ggtgatacca gacttgtagt catggttgcc
     1441 aagcacagcg tagacaggag catcgatccc ctcccatatt ttttggagcg agaggtcctc
     1501 tccatccccg tagacgaaat cccccccgat catgactatc gacgggtcaa gggcgttgat
     1561 ctcattgatg atggtattga cgtagttgat attcccctgt ttcaggtggg ggtcagcaat
     1621 aaagacgata ttgtctggtg ctccgtcgat atggagcacc gtgatctccg aggtcttcgc
     1681 ctcgacatag gcaaggccta tcgaggcatt gaggatgccg agaaaaacga tgaacgtaag
     1741 gtagtgggtt gtcccaatct ttggcatctt cggaagtgcg tctttcaatc gcatttatac
     1801 cttttgtttt ggtgatgatg gataaaaatg gtgtgggtgg tggcagcaga gaaacgccag
     1861 aaccgatgtc tgttctggaa aaaaagtgag agcgccgggg aagagatttg aactcttgag
     1921 gtgcatggca ccagtggctt tcaaggccac cgccttaccg gtctagacta ccccggcccg
     1981 gatatagtat atgcgatgcc aaagcataat aaaaagatca ccagtttctc cggatcacaa
     2041 cgagtgcaac cccggcaata accgcggcta ccacccctat agccggcaga ggggaggatt
     2101 tttcttccgt ggaaggggta cctggcgtgg ttgcagcagt accggggcct gacgtctcca
     2161 ccttcaccgc cccatctgcc gacaggagca catccccctc agctgcgtaa ctgtaagggt
     2221 acaccttcag gacaagaaca tcctcagcgg tgtcgatgat gatctcctca tttcccaccg
     2281 aacctttttc gtttactttc accgtcttcc cttcggtatc ggtatactcg ataacccagt
     2341 cgatccctga cgaggtgaaa gccctgatcg tccctggtcc acccgtggtc actacgaaag
     2401 tcgccggctt gtccctggag gcaaaggcac ttgctgacag gatcggattt gtggtgggga
     2461 cgatggttgg cggggtggtt gctacaggct tctcccggac cgtgaccata atcgtcccga
     2521 tgtatccctt ggcatcggtg aaactgatat tatactgccc gggacccgtg atagggacct
     2581 ctgtggagaa cgagctgtct gacttgacac taacataggc cggtccgaac accgcttcac
     2641 ctcccgggcc agtgactccg atctggaccc ccgaatttgc cagtccggca atggatccgt
     2701 caatatcgag ggtcccgtcc atctcctggg aactgaacgc tgaaaagacg atctcgtcgc
     2761 tgcggtcgat gatctcgatc acccggaggg ttaccgaatc ccccaggtag gaatacccgg
     2821 agatggcagg cacctccgcc ttgtataccc ccttggtaaa tcctgtggtg tcaaatacga
     2881 cagaaaattc attgtttgcc tggagcgtga ccgtttttcg tgcgagttcc tcggtaacat
     2941 ggcctgattt ataaaggaca atgtcgacgc tgatgccagg aggaaggttg cttgttccgt
     3001 tcaccacgag cggcatcccc ctctggatct gcgagggcgc cttgagtatc agttcataag
     3061 cctgtgcaac tcccccgcag agggcaaggc acaggagggc cacgagagcg attcgcgcca
     3121 gggtgtgaga ggctggtctc ttctggtcca tcaataaatc ccgcctgaat gcatgggtgt
     3181 tgtcgtcagg ataaaaacga caccagttgg ataaaaattt gtgctgcatc cttaatacct
     3241 tgcccatccg gcatgccgtc cccgttgatg ctttaaacct gcacgaccca tgaaagaggt
     3301 gttgtaatca tggaacggcc gctcgcatgc tggaaagggg aggaacgata cgaaggagag
     3361 gtcctcccct cgctcaccat catcatgaag accggggggt gccggcacaa ccgctgcagg
     3421 atgtgcagtt accggtttga acggtacccg gaggggaccg atctcgaggg cgccctcctc
     3481 tcccagctcc actgggtggt ccagcattac cctccatccg gggttgcgat cgtcaagctc
     3541 tacacttcgg gcagtttctt cgatcccggg gaagtgcctc cccgcgtggt agaggaggcg
     3601 gcccggatct tttggggaaa acttgtcatc gcggagacac ggcctgaatt tgttgaccct
     3661 gaacggattg cagccttcat ttcagcgatc gattcgggag attacccgac cccgctctac
     3721 gtagccatcg gtctcgagac cctgaatgac agtatccggc aaaaatcgat cgataaggga
     3781 tttacgttct ctgatttcaa aaaagcggca gataacgcaa gagcgggggg cgccgggatc
     3841 aaggcatatc tccttttaaa gccgctcttt ctcacggaac gggaggcgct cctggacatg
     3901 gaggagtcga tccggaccgt tactggaatt gctgatatga tctcgatgaa tccctgcacc
     3961 gtccagaagc ataccgagct cgaatactac tggaagcggg gagagtaccg gcctccctat
     4021 ctctggagtg tcgtttcatt gctcctctct gcacccgccc atatcacctg tgatccatta
     4081 ggagggggtc gtgcccgcgg acctcacaat tgcgggacct gtgattatga tattgtcaag
     4141 gcaatacggg attacaacct ctctgcggat tgccagacgc ttcaccagct cttttccggg
     4201 ggatgcgaat gcaaagaaga atgggagcgg gtcctgtcac tcgagaaacc gtactgcatg
     4261 ccgctgactc ggtgatggca cttcctggat ctcgagctgc ttttcgagga ggggcaggaa
     4321 tgtcccggca tcgcttacca ccccgatagc ctgcatggtc ccgcggtcca tcagctttgt
     4381 cagggatgag ggattgatgt cgacgcagat ggttttcaca aaggagggga ggcagttgcc
     4441 gactgccacg gagtggagga gcgtggctac catgagaacc atcccgcacc cctccacatg
     4501 agaacgaatc tggtcctggg ctaccactgc atcagtgatg acatcaggca acggaccgtc
     4561 gtcacggata gatcctgcaa ggacgaaggg aatatccttc atgacacatt cgtacatgat
     4621 cccgcccgtg atcaccccgg tttcgatcgc tttttttatc gaacccgccc gcatcacttc
     4681 ggaaatggca tacaggtgat tcttatgacc tcccgtgacc ggttttcctg tacagatgtc
     4741 cataccaagc gatgtcccgt aaaggttgta ttcgatgtca tgggtggcaa gggcattgcc
     4801 ggcaaagagg acatcgatgt atcctttccg gataatcgat gcaagggcac gggcggctcc
     4861 ggtatggatg atcgcaggtc cccccacgag tccgatcttc cctccctttg ctttcagtgc
     4921 gatcatctcg gcggcaacct tcgcgatgat cgtctcgctt ggccgttcgg gtgagacggt
     4981 gccgtgcatg aactcgaaat tggagagctg gcggggacgc tcgggagggt ccacccgcac
     5041 tccctcttca ccaaccacca caagatcgcc tttcttcagc tttgagagcg gggtgcacag
     5101 ggctgacttc ccctcgggat cgaccacgat cgtgcagtcc atctcgatgt gctcaaccag
     5161 gatccatcgc ccccggtatt tgacagaagt cgggtggtgg gtggtcgagt aaaatccttt
     5221 cgggacgata cgatcaccct ccgcaggggc aaccgaaatc tcctcttcaa agagcaggtg
     5281 ggcaccgaac cggtggatct ctgagaggat cgcctggagc tgctgttcgg tatcggtatt
     5341 cactttcagg cgtgcatagc ttgggtctgt cttccgtttg cctacttcaa aggtgataat
     5401 ctcaaagttg ccacccttgt ccatgatccg atcaaagacc tttgtcatga tgcccgagtc
     5461 gatgatgtgc ccctcgagct cgacttcacg ggagacctgc atagtaagta taaatcagcg
     5521 gcgcttgctt atattatttc tccggtaatc gggggaatct gaccaaactg actcgtttgt
     5581 attgttttta gagggcttat cggtcctggc agagctggcg aaccctgtcc agttcttccc
     5641 gggtattgat gttgtatgca aggctctcct cccagaggag gagctgcagc tcatcctgag
     5701 gtttctccat gagatccc
//
LOCUS       DAOOCO010000128         5717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13295_length_5717_cov_4.286471, whole genome shotgun sequence.
ACCESSION   DAOOCO010000128 DAOOCO010000000
VERSION     DAOOCO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13295_length_5717_cov_4.286471"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="argH"
                     /locus_tag="PK154_07620"
     CDS             complement(<1..1192)
                     /gene="argH"
                     /locus_tag="PK154_07620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW10964.1"
                     /translation="MGNDVVRRGRLSGERTGEMIHFLSSMAADRHIAEADILVDIAHV
                     MMLSKQRILDEATTRPLLAALLKLYDEGIPESVFDDEYEDIHAGIEAFLIAMVGEETG
                     GRMHMGRSRNDEVAACLRIRLREDLIRQLSLLIRLRQTLLSLAEEHRETVMPGFTHLQ
                     YAQPTTLAHHLLAYEQVFSRDCERLSEGYSRVNQSPLGAAAFASTGYPVNREYVAQLL
                     GFDGVLENTMDAVSSRDFALETLSSLSIMMVGVSRLCEELIIWSSPFAGFISLNDVFC
                     STSSIMPQKKNPDFAEIMRAKAASLIGAYMSAAALMKGLPMSYNRDLQELTPSLIRGI
                     EDARQSTSLLNGMLLTASFDRERMREEAGKSYSTATDLADMLVRRCGLPFRTAHSIVG
                     RAVQN"
     gene            complement(1234..1737)
                     /locus_tag="PK154_07625"
     CDS             complement(1234..1737)
                     /locus_tag="PK154_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW10965.1"
                     /translation="MRAALISDQEFVRTLNQIMKNEVRISVRELAEKSGIAQSSLYKI
                     LHGHRSPNLSTLRAIVHALQYFERLGEGEFIGLIAARPVLDTVQERNAEVDGHRIRIR
                     EYPVHTMEDAIVAAVRAEREGATAIVCAPIVSSIIEQLVHIPVATIIPRESVQRAIEL
                     AARKAWL"
     gene            1959..3161
                     /locus_tag="PK154_07630"
     CDS             1959..3161
                     /locus_tag="PK154_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW10966.1"
                     /translation="MIKRLIVSLAVMLTALLIAGCITPSPSGENSVSVIYTKGTGPMP
                     TLLATDQIDGYIAWQPFVEVAPLAGIGKVLVYSGDLPPAGRWKNHPCCVFTARQDAID
                     DNPELTNALTAAFVLSTQYISEHPNESAEIVADWLAGKGNFTYGNITVSSVDVLQRAF
                     PTVKFVNEPTDEWKNDNLEFVYAQRELGVLTGALVNTTDAESLRLLFDTRPYESAQVM
                     IQSGQITTPPPQSKPVSVGYLLSDHDAALFVAVKNWKYFQDTYRIALKPRDLTASRPD
                     IADLIVNGVTVAEVRLVPADAGPQLMQLASTDTIQYALVGNPPTIAAIDKGTPVKIIM
                     ALNEEGSGIVIAEDAPANDWESFVSWAETRSAGGQPVKIAAPGKGSIQDVLLRYALEE
                     SGLSIKEG"
     gene            3175..3942
                     /locus_tag="PK154_07635"
     CDS             3175..3942
                     /locus_tag="PK154_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW10967.1"
                     /translation="MRSPRILLLPVLLIVGWEVAAYFIDNPFVLPSLSTVIPILLHPF
                     STEYTLGTGSLVENAAASLFRVASGFLLAAIIAIPLGIGMGRSSFLHDFLDSTIELLR
                     PIPPLAWVPLALAWFKIGFVSIVFIIFIGAFFPILLNTIDGVKSVKKTWVEVAETLGA
                     RETDVLTKVILPGSAPTVWTGLRVGFGIAWMCVVAAEWMPGISLGLGYLILYAYNFGQ
                     TNLIIAGMVVIGVIGILIDQVFRAGERRWFSWRSLER"
     gene            3977..4765
                     /locus_tag="PK154_07640"
     CDS             3977..4765
                     /locus_tag="PK154_07640"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW10968.1"
                     /translation="MTLIIQDLSKEFPVPNQAPILSLSKISLTVGEEEFVSILGPSGC
                     GKTTLLRIIAGLDSATSGSITLDGETIDRRSSQMAMIFQEYSLYPWRTVIDNVTFGLE
                     MQNTAKRPARDTAMKYLNLVGLAEFAERFPYELSGGMRQRVAVARAMAIEPKILLMDE
                     PFGALDAQTRNMLQKELLEIWEKTKKTVLFVTHSVDEAVYLSDRIIVLTPRPGRIREI
                     VENPLARPRDRTSPEFARIRRHVLDLINESSQPKEDLIVHPPSL"
     gene            4797..5351
                     /locus_tag="PK154_07645"
     CDS             4797..5351
                     /locus_tag="PK154_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPW10969.1"
                     /translation="MVRKPAKMYRNLAKKAYTRREYMGGVPGSKVVQFDMGNLSQEFP
                     LELNMIVEESCQIRHSALEAARISINRKLLKEVGRMNFHLKLRVFPHHVLRENKQATG
                     AGADRVSEGMRLAFGKAVGTAARVMKDQAVFTTYTTPQYLDKVKLAMKAGSHKLPSPS
                     YFTVVDRSSSAPSEIPSQAVNQPE"
     gene            5426..>5717
                     /locus_tag="PK154_07650"
     CDS             5426..>5717
                     /locus_tag="PK154_07650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW10970.1"
                     /translation="MVLPEDDPLFRALSAATCRAFREAVTTLPQDVVEALRSAAMREP
                     SALAKNELKNILDNIAIAEQRALPLCQDTGVPVVYLTVPSHVPFTDTLICAVG"
BASE COUNT         1412 a         1494 c         1453 g         1358 t
ORIGIN      
        1 cgttctggac cgctctcccg acaatactat gggccgtgcg gaaaggaaga ccacatctcc
       61 tcacgagcat gtcggctaaa tccgttgcgg ttgagtagct ttttcctgct tcctccctca
      121 tccgctcacg gtcgaacgag gcagtcagga gcatcccgtt cagcaggctg gtgctctggc
      181 gtgcatcctc aattccccgg atgagagagg gggtgagctc ctgaagatcc cggttatagc
      241 tcatgggaag ccctttcatg agcgcagcag cggacatgta tgcgccaata agtgaggctg
      301 ccttagcacg cataatctca gcaaagtccg gatttttctt ctggggcatg atagaactcg
      361 tggaacagaa aacatcattg agcgagatga accctgcaaa cggagaactc cagatgatca
      421 gttcttcaca gagccggctg acccctacca tcataatcga gagactggag agcgtttcaa
      481 gggcgaaatc ccgtgatgaa acggcatcca tggtattttc aagtacacca tcaaaaccca
      541 gcagctgcgc aacatattct ctgttcaccg gatatcctgt tgatgcaaaa gccgcggctc
      601 cgagaggaga ctggttgacg cgggaataac cttcggacaa ccgttcacag tcccgggaga
      661 atacctgttc ataggccaga aggtggtggg cgagcgtggt aggctgggca tactgcagat
      721 gggtaaatcc aggcattacc gtctccctgt gctcttcggc aagggagaga agtgtctgcc
      781 tcaacctgat gagaagagag agttgccgga tcagatcttc tctcagtcgg atacgcagac
      841 aggcggctac ctcatcattc cgcgatcttc ccatatgcat ccttccgccc gtctcttcac
      901 ctaccatggc gatcaggaag gcctcgatcc ctgcatgaat atcctcatac tcatcatcga
      961 atactgactc gggtatcccc tcatcataca gtttgaggag tgcagcgagc aatggccggg
     1021 ttgtcgcttc atcaaggatc cgctgtttgg acagcatcat cacatgcgct atatcgacta
     1081 ggatatcagc ttcggcgata tgccggtcag cagccataga tgagagaaaa tgtatcatct
     1141 ccccggtccg ctctccagag agccgccctc tccgcaccac atcattgccc atccgaatta
     1201 tacctctggt actaagagga acgggaaaaa agattaaagc cacgccttgc gggcagcaag
     1261 ctcgatggcg cgctgcaccg attcacgcgg gatgatagtg gctacaggaa tatgcaccag
     1321 ctgctcgata atgctggaga ctatcggggc acatacgata gccgtagccc cttcgcgttc
     1381 tgcccggact gcagccacga tggcgtcttc catggtatgt acagggtact cacgaatcct
     1441 gatgcgatgc ccgtccactt ccgcgtttct ttcctgaacg gtatcaagga ctggccttgc
     1501 agctatcagg ccgatgaact ctccttctcc caaccgttca aaatattgaa gagcatgtac
     1561 aatagcacga agagtggaga gattgggtga acggtgacca tgaaggatct tgtataggga
     1621 actctgggca ataccactct tttcagcgag ttcgcgtaca ctgatcctca cctcgttctt
     1681 cataatctga ttgagcgtcc gtacgaactc ctggtcagat atgagagcgg cgcgcataag
     1741 acggtttaca ggatcactca gcaccatgag tatacagaag ggataatgtg tatcgacata
     1801 taacattttt gtccaaattg taattgtatt tggccaatta tggacagata aatcataata
     1861 gtgatattat tgtataatat tttttaataa ttaatacaaa tatagaattt aattgggtca
     1921 ataggagaat ttatactcca ctaatatgag tattattcat gataaaaaga ctgatagttt
     1981 cccttgccgt gatgctgaca gcactcctta ttgcgggatg catcactcca tcaccatcag
     2041 gggagaattc tgtatcggtg atatacacca agggcactgg cccgatgcct acccttcttg
     2101 caaccgatca gatcgatgga tacattgcct ggcagccgtt tgtggaggtt gcacctcttg
     2161 ccgggatagg caaggtcctc gtttactctg gcgatctccc ccctgctggc aggtggaaga
     2221 accatccctg ctgtgtcttt accgcacggc aggatgccat cgacgacaat cctgaactca
     2281 ccaatgccct gacggcggct tttgtactct ccacccagta tatcagtgaa cacccaaatg
     2341 agagtgcgga gattgtggca gactggcttg ccgggaaggg caacttcaca tatggaaaca
     2401 tcactgtctc ttccgttgat gttctccagc gggcattccc cacggtcaaa tttgtgaatg
     2461 agccgaccga tgaatggaag aatgacaatc tggaattcgt gtatgctcag agggaacttg
     2521 gcgtcctgac tggcgcgctt gtcaatacaa ctgatgccga atcactcagg ctcctcttcg
     2581 acacccggcc ttatgaatca gcacaggtaa tgatccagtc cgggcagatc actactccgc
     2641 caccccagtc aaaaccggtc tctgtcgggt acctcctttc cgaccatgat gcagcattgt
     2701 ttgtcgcagt aaagaactgg aaatatttcc aggatactta caggatcgcg ctgaaaccaa
     2761 gggatcttac cgcatcccgc cccgatatcg ctgaccttat cgtgaatggt gtgactgtgg
     2821 ccgaggttcg gcttgtcccc gcagatgccg ggcctcagct gatgcaactt gccagtactg
     2881 ataccatcca gtatgccctt gtcgggaacc ccccaacgat tgcagcgatc gacaaaggaa
     2941 cacctgtgaa gatcatcatg gctctcaatg aagaaggatc agggatagtc attgcagaag
     3001 acgcccctgc caacgactgg gagagctttg tctcatgggc cgaaacccgg tcagccggtg
     3061 gccagcctgt aaagatagca gccccgggaa aaggatccat ccaggacgtg ctccttcgct
     3121 atgcccttga agagagtggt ttatcaataa aggaaggata acgtttagag aataatgagg
     3181 agcccccgta tcctgctcct cccggtcctt ctaatcgtgg gatgggaggt ggcagcatat
     3241 tttattgata atccgtttgt tctcccttca ctcagtacgg ttattcctat cctcctccat
     3301 cccttttcca cggaatatac tcttggaacc ggaagtctgg ttgagaatgc agcggcgagc
     3361 ctctttcggg ttgcatcagg atttcttctc gccgcaatca tcgctatccc gctggggatt
     3421 gggatgggca gatccagctt cctccacgac ttcctggaca gcaccattga acttcttcgg
     3481 ccgatacccc cgctggcatg ggttcccctt gccctggcat ggttcaagat cggttttgtc
     3541 tcgatcgtgt tcatcatatt tatcggagca ttcttcccga tactcctgaa cacgatcgac
     3601 ggtgtaaaat cggtcaagaa aacatgggtg gaagtggcag agacacttgg tgcccgggaa
     3661 acggacgtac ttacgaaggt gatccttccc ggttcagctc ctacggtatg gacagggctc
     3721 cgggtggggt tcgggatcgc atggatgtgt gtggttgcgg cagagtggat gccaggaata
     3781 tcccttggac tcggctacct tatcctttat gcctacaatt tcggacagac gaaccttatc
     3841 attgcaggga tggtcgttat cggggtgatt gggatcctta tcgatcaggt cttccgggca
     3901 ggggagcgga gatggttctc atggagatcg ctggaacgct gactcatgcg gcaatttttg
     3961 atggagtgaa gaaagaatga cccttataat ccaggatttg tcaaaggaat tcccggtacc
     4021 gaaccaggca ccaatcctgt cactctcaaa aataagcctc acggtggggg aggaggagtt
     4081 tgtatcgata ctcgggcctt cagggtgcgg gaagacgacc ctcctccgga ttattgccgg
     4141 gctcgattct gcaacttccg gttcaatcac ccttgacggt gagaccatcg atcgccgcag
     4201 ttctcaaatg gcgatgatat ttcaggaata ttcactgtac ccctggagaa cagtgatcga
     4261 taacgtcacc ttcggcctgg agatgcaaaa tacagctaag aggcctgctc gcgacactgc
     4321 aatgaaatac ctcaatcttg tagggctagc tgagttcgcc gaaaggtttc cttatgaact
     4381 ttcgggcgga atgcgtcaga gggtagccgt ggcacgtgct atggcaatcg aacctaagat
     4441 tcttctgatg gacgaacctt ttggagcgct tgacgctcag accaggaaca tgctccagaa
     4501 agaattgctt gagatctggg agaaaacaaa aaagactgtg ctctttgtta cccacagtgt
     4561 cgatgaggcc gtctatctct ctgacaggat tattgttctc actccgcggc caggacgcat
     4621 ccgcgagatc gtcgaaaatc cacttgccag gccaagggac cgtaccagcc cggaatttgc
     4681 gcggatacgc cggcatgttc ttgaccttat caacgagagc agccaaccaa aggaagattt
     4741 aatagtgcat cccccctcat tataaagaac cgataataaa aaggagtgac tgactgatgg
     4801 ttcgaaaacc agcaaaaatg tacaggaatc tggcaaagaa ggcatatacc cgaagagaat
     4861 atatgggtgg tgtccctggg agcaaggttg tccagttcga catgggaaat ctttcccagg
     4921 aatttcccct cgagctgaac atgatcgttg aggagtcctg ccagatacgc catagtgcgc
     4981 tcgaagcggc acgtatcagt ataaaccgga agctcctcaa agaggtagga agaatgaatt
     5041 tccacctcaa actcagagta ttccctcatc acgtcctccg cgagaacaag caggctaccg
     5101 gagctggcgc agaccgtgtc tctgaaggga tgaggctggc attcggaaaa gcagtgggaa
     5161 cggctgcccg ggtaatgaaa gatcaggcgg tcttcacaac ctacaccacc ccccagtatc
     5221 tcgacaaggt gaaacttgcc atgaaagcag ggagccacaa gctcccgtca ccctcgtatt
     5281 tcactgtagt ggatcggagc agcagcgcac cttccgagat cccgagccag gcggtcaacc
     5341 aacctgaata agaccattac tatcttcccc ttttttctga ttccagacag aaacgattat
     5401 agattttctt cgccgagtac tggtgatggt tcttccggaa gatgacccgt tattcagggc
     5461 attgtctgcc gccacctgcc gggcgttccg ggaggctgtg acgacccttc cccaggatgt
     5521 tgttgaggca ctgcgctctg ctgccatgag agagccttca gcactcgcga agaatgaact
     5581 aaaaaacatc ctcgataata ttgccattgc agaacagcgt gccctccctc tctgtcagga
     5641 caccggtgtt ccggtggtat atctgacagt gccatcgcat gtgcccttta cggataccct
     5701 catttgtgct gtaggag
//
LOCUS       DAOOCO010000129         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13349_length_5699_cov_5.417257, whole genome shotgun sequence.
ACCESSION   DAOOCO010000129 DAOOCO010000000
VERSION     DAOOCO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13349_length_5699_cov_5.417257"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK154_07655"
     CDS             <1..302
                     /locus_tag="PK154_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-ribosyltransferase"
                     /protein_id="HPW10971.1"
                     /translation="DFLKIETGRVAGYLEKTRDAYRYRIGYCLGLFREALIRGSERAG
                     VPIDLVLPSRVKINKIIEEEDCSFQEGSLSMDEYLGEFCEELIRFRNSLERDSSR"
     gene            complement(460..747)
                     /locus_tag="PK154_07660"
     CDS             complement(460..747)
                     /locus_tag="PK154_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPW10972.1"
                     /translation="MIYVKVTVRFFARFGELLGNTQEIEAREGISVADLVKEAASRNK
                     AGYDTIFNEEGAFRDFVIVMRNKKRIRSQDAGSIPVGEGDEIAVFPPVAGG"
     gene            complement(744..1445)
                     /locus_tag="PK154_07665"
     CDS             complement(744..1445)
                     /locus_tag="PK154_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPW10973.1"
                     /translation="MMLFGEEGQEQLQDATIFIAGAGGLGSPVSIYLAVLGVGTLIIA
                     DNDRVDQTNLNRQILYRDQDIGRRKAAVAGERLQEVNPDIMVKAVDATIDQHTVSSLT
                     RDADGIVDALDDFPTRYLLNKAALEKGIPLFHGGISGFFGQATTILPGKTPCLRCIIP
                     QPPPAEVFPVAGVTAGFIGLIQATEVCKYLLCRGDLLAGRLLLWDGIQGRIEEIPVET
                     DPDCEACAGKYKGIS"
     gene            complement(1468..1608)
                     /locus_tag="PK154_07670"
     CDS             complement(1468..1608)
                     /locus_tag="PK154_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10974.1"
                     /translation="MPLRRTPEYTRQVNMRVRSVDRSVFLTASGKGIERMRGGDIPAL
                     RS"
     gene            1754..2590
                     /gene="larE"
                     /locus_tag="PK154_07675"
     CDS             1754..2590
                     /gene="larE"
                     /locus_tag="PK154_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPW10975.1"
                     /translation="MTGTTLEERMEQLRRRISGCGQLLVSFSGGVDSGLLAALGKEIL
                     GDNVQAVFIDSPLVPSSAVLEAREMAGLLGIPLGIITAPFPGEMVSWNLPDRCYHCKK
                     SFSELLRAWADEQGISCIADGVTESDRLEYRPGTRAATEAGIIHPFLESRITKAEIRQ
                     LARERGLPFWDKPSTACLASRIPYGEALTRENLRMVEEAEAGLAGAGFAGSRVRNHGG
                     IARIEVPETDITRLVAAREKIVSLVKQAGFSYVTLDLIGYRSGSMDEVRGKTAVPGKE
                     GS"
     gene            2590..3399
                     /gene="thiM"
                     /locus_tag="PK154_07680"
     CDS             2590..3399
                     /gene="thiM"
                     /locus_tag="PK154_07680"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPW10976.1"
                     /translation="MDGTVYDNLFALVRKERPLVHHITNRVTINDCANITLCAGAAPV
                     MAEAPEEAAEMAGLAGALVLNIGTLTEAQVGAMLLAGARANEHGIPVILDPVGAGATR
                     LRTETVLLLLDKLEVAILKGNAGEIGVLSGLGGTVRGVDSGGVSGDPVEVARECARAT
                     GTIVAMTGKVDVVADGRRTFLVANGTPMMGNLSGTGCMAASVTGAFAAVADDMTAAPV
                     AALVAFGLAGERAMKGCSGPYSFRTALFDAMYRLGPADLAVGAKVSVPDGI"
     gene            3389..4033
                     /gene="thiE"
                     /locus_tag="PK154_07685"
     CDS             3389..4033
                     /gene="thiE"
                     /locus_tag="PK154_07685"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPW10977.1"
                     /translation="MAYDLYVITDPAIARGMPHGGIARRVVEGGADAVQLRDKTLPGR
                     DLLAVACEIREITEESGVLFFVNDRIDIALAAGADGVHLGQGDLPVREARSISPEGFI
                     IGVSVGSVSEAVAAESAGADYLSLSPIFPTTSKEDAGPGHGLVVLQAICASVTLPVIA
                     IGGITPRNVPEVIAAGAEGVAVISAVIAQEDITAAARHMKAIIRDSKERVRQVR"
     gene            complement(4044..4955)
                     /gene="nadA"
                     /locus_tag="PK154_07690"
     CDS             complement(4044..4955)
                     /gene="nadA"
                     /locus_tag="PK154_07690"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPW10978.1"
                     /translation="MAEDSELREEILALKAERDAVILAHNYQIDAVQDIADFVGDSLE
                     LARAAAAQKAPVIVFCGVDFMAETAAILSPEKTVLLPAAGACCPMADMVTAGELAVLQ
                     EQYPDAAVVAYVNTTAEVKAASDICCTSANAVKVVESLSEHQVIMVPDRNLARYVARF
                     TTKEILPWDGFCLVHERFTGKDIQRARSRHPGAEVLVHPECRPEVIDAADQVFSTSGM
                     IRHICGSKNEKFIIGTETGILHQISLKCPEKHCYPLSSRAICVNMKKTDLEKVHAALD
                     RMAPRVGVPERIAIPARRAIERMLALP"
     gene            complement(5305..>5699)
                     /locus_tag="PK154_07695"
     CDS             complement(5305..>5699)
                     /locus_tag="PK154_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW10979.1"
                     /translation="IAGGIGLGVAAEYLMAKGMERIHRHESTLATRLIDGLQGIVSVR
                     VYAPRDPDARIGVVSFTIEGMHPHEIAQQLDDQAEILVRSGYHCCQPLMESLGLPDGT
                     VRASLALYTTEQEIDLLLAAVRAISRGR"
BASE COUNT         1138 a         1626 c         1783 g         1152 t
ORIGIN      
        1 aggatttcct gaagatcgag accggaaggg tggcaggata cctggagaag acacgggatg
       61 cataccggta ccggatcggg tactgcctcg ggctgttccg tgaagcactc atcagggggt
      121 cggagcgggc cggggtgccg atcgatctgg tcctgccgag ccgggtgaag atcaacaaga
      181 tcatcgaaga ggaggactgt tcgttccagg aaggcagtct atccatggat gaatacctgg
      241 gcgagttctg cgaggagctg atccggttcc ggaattctct cgagagagat tcgtccaggt
      301 gatcctccct ggaagattgc gatgcggttc tctgtcagtt ctatcaaaaa gatggcgaag
      361 caggagttcg gggacctcct gaacgaggct tttggagtta gcaggagtgg tgagggaccc
      421 gggaggcagg aaaaaaagat tgtatcccaa agggccggat tacccgccgg caacgggagg
      481 gaagaccgct atctcatccc cttcaccgac cgggatggag ccggcatcct gtgaccggat
      541 cctctttttg ttccgcatca cgatcacaaa atccctgaat gcaccttctt cgttaaagat
      601 cgtgtcgtac cctgctttat tcctggatgc tgcctctttt acgaggtcag caaccgagat
      661 gccttccctg gcctcgatct cctgggtgtt cccgaggagt tctccgaacc gggcaaaaaa
      721 tcggacggta accttcacgt atatcacgat atccctttat attttcctgc acaggcttcg
      781 caatctggat ccgtttcaac cgggatctcc tcaatccggc cctggatccc gtcccagagc
      841 aggaggcggc cggcgaggag atcaccccgg cagaggagat atttgcagac ctcggtcgcc
      901 tgtatcaagc caatgaatcc cgcggtcacg ccggcaacgg gaaagacctc ggcgggtggg
      961 ggctggggga tgatacagcg caggcagggg gtctttccgg ggaggatcgt ggtcgcctgg
     1021 ccgaagaagc cgctgatccc cccgtgaaag agggggatcc ccttctccag ggctgccttg
     1081 ttcaggaggt accgtgtcgg aaaatcatcc agggcatcga caatgccgtc agcatccctg
     1141 gtcagggagg agaccgtgtg ctgatcgatg gttgcatcaa ctgctttcac catgatgtcg
     1201 gggttgacct cctgcaaccg ttctccggcg accgctgctt tccgccgccc gatgtcctgg
     1261 tcgcggtaga ggatctggcg gttcaggttc gtctggtcca cccggtcatt gtccgcgatg
     1321 atgagggtac ctaccccgag gacagcaagg tagatcgata ccggcgatcc aagccctccg
     1381 gccccggcaa tgaatatggt ggcatcctgc agctgttcct gcccctcttc gccaaaaagc
     1441 atcatctgcc tgcggtagcg ttcacgctca cgagcggaga gcagggatat cgcctcctcg
     1501 catccgctct attcccttgc cgctggcggt caggaatacg cttctgtcaa ccgatcgcac
     1561 cctcatgttc acctgccggg tatactccgg agttcttcgt aatggcataa aaaatgcacc
     1621 atcaccggat tctgtctcca ttataatcag agatgggaaa gtggataatc ttgtatcccg
     1681 aacctttggt accaaaagga gcatgattgc ttctgcaacg agaaccatat ccctgtaaag
     1741 acaggtgcgc aggatgaccg gtaccacact cgaagaacga atggaacaac tgaggaggcg
     1801 gatctccgga tgcggacagc tgctggtctc tttctccgga ggggttgaca gcggcctcct
     1861 tgcggcactg ggaaaagaga tactcggtga taacgtgcag gcggtcttca tcgacagccc
     1921 cctggtcccg agttcggctg tattggaagc ccgggagatg gctggactcc tcgggatacc
     1981 gctcgggatt atcacggccc cgtttcccgg ggagatggtg tcatggaacc ttccggaccg
     2041 ttgttatcac tgcaagaagt cattctcgga actcctccgg gcatgggcag acgagcaggg
     2101 catatcgtgt attgccgatg gggtcacaga gtccgatcga ttagaatacc gtcccggtac
     2161 ccgcgcagcg accgaggcgg gaatcatcca cccgtttctc gaatcccgga taacgaaggc
     2221 cgagatccgg cagctcgccc gggagagggg gctcccgttc tgggacaagc catcgaccgc
     2281 ctgccttgcc tcacggatcc cgtacgggga ggcactcacc cgggagaatc tccgcatggt
     2341 ggaggaggcc gaagccggtc tcgcaggagc gggattcgcc gggagcaggg tccggaacca
     2401 tggcggcatc gcgagaatag aagttccgga aacagacatc acccggttgg tggcagcacg
     2461 ggagaagatc gtctccctcg tgaagcaggc cggcttctcc tatgtcacac tcgacctgat
     2521 cgggtaccgg agcgggagca tggatgaagt acgggggaag acggcagttc ccggaaagga
     2581 gggctcctga tggacgggac ggtgtatgac aacctgtttg ctctcgttcg caaggagcgg
     2641 ccgctggtgc accatatcac gaaccgggtc accatcaacg actgtgccaa catcaccctc
     2701 tgcgcaggtg ctgccccggt catggcagag gcaccggagg aggcagccga gatggccggg
     2761 ctcgccggag cccttgtcct gaatatcgga acccttacag aagcccaggt cggggcgatg
     2821 cttcttgccg gtgcacgggc aaacgaacat gggatcccgg ttatcctgga cccggtcggt
     2881 gccggggcca ccaggctgcg gactgagacc gtcctcctgc tcctggataa gcttgaggta
     2941 gctatcctga aggggaatgc gggagagatc ggggtgctat caggtcttgg gggaacggtc
     3001 aggggggttg attcgggcgg agtatccggg gacccggtcg aggttgccag ggaatgtgcc
     3061 agggcaaccg ggacgattgt cgcgatgaca ggcaaggttg acgtggtcgc tgatgggcgc
     3121 cgcaccttcc ttgtcgcaaa cggcaccccg atgatgggga acctctcggg gaccggctgc
     3181 atggcggcct cggttaccgg tgcgtttgcc gcggtcgcgg acgacatgac tgcagccccg
     3241 gtggcagccc tcgtggcgtt cggccttgcc ggggagaggg ctatgaaggg gtgctcgggg
     3301 ccgtactcgt tccggacagc attgttcgat gcgatgtaca ggctggggcc ggctgatctt
     3361 gcagtggggg cgaaggtgag cgtgccggat ggcatatgat ctctacgtga tcaccgaccc
     3421 ggcgatagcc cgcgggatgc cccatggcgg gattgcccgc cgggtggtgg agggaggagc
     3481 cgatgccgtc cagctccgcg acaagaccct tcccgggaga gacctgctcg cggttgcctg
     3541 cgagatccgc gagataaccg aagagtcggg agtactcttt tttgtcaacg accggatcga
     3601 tatcgccctt gccgcaggag cagacggggt ccacctgggg cagggggatc tcccggtcag
     3661 ggaagcccgg tcgatctccc cggaagggtt catcatcggg gtatcggtcg gcagcgtcag
     3721 tgaggcagtc gctgccgagt ctgccggggc agactacctc tcgctcagcc cgatctttcc
     3781 gaccacgtca aaggaggatg ccgggcccgg ccatgggctt gtagtgttac aggcgatttg
     3841 tgcatcggtg acactccccg tgatcgcgat tggcgggatc actccacgga atgtgccgga
     3901 ggtgatcgct gccggtgctg aaggggtcgc tgtgatctca gcggtcattg cacaggagga
     3961 tatcacggcg gctgcacggc atatgaaagc gatcatcagg gattcgaaag aaagagttcg
     4021 gcaggtccga taaccttcct gcattacgga agagcaagca tccgctcgat agccctccgt
     4081 gccgggatcg cgatcctttc agggaccccg acccggggtg ccatccggtc gagggcggca
     4141 tggacctttt ccaggtccgt ctttttcatg ttgacacaga tcgcacggga ggagagcgga
     4201 tagcagtgct tctccgggca tttgagggag atctggtgca ggatcccggt ctcggtgccg
     4261 atgatgaact tctcgttctt actcccgcag atatggcgga tcatcccgga cgtggagaag
     4321 acctggtcgg ctgcatcgat cacctcgggc cggcattcgg gatggacgag cacttctgca
     4381 ccggggtggc ggctcctcgc cctctggatg tccttcccgg tgaaccgttc atgcacgagg
     4441 cagaacccgt cccacgggag gatctccttg gtggtgaacc tggcgacgta ccgtgcaaga
     4501 ttccggtccg gcaccatgat cacctggtgc tctgacaggg attccaccac cttcaccgcg
     4561 tttgctgaag tgcagcagat gtcactcgcg gccttcactt ccgcggtggt gttaacatag
     4621 gcgaccacgg cggcatccgg atattgctcc tgcagaacgg caagttcacc ggccgtgacc
     4681 atatctgcca tcgggcagca ggcaccggca gccggaagga gtaccgtctt ttccggggag
     4741 aggattgcgg cggtctctgc catgaagtcc acgccgcaga acacgatgac aggggctttt
     4801 tgcgcggcgg cagcccgcgc cagctcgagg gaatccccta caaaatcggc tatatcctga
     4861 acggcatcga tctggtaatt atgggctagg atgaccgcat cccgttccgc cttgagtgcg
     4921 aggatctcct ccctcagttc actatcttcc gccatactac ctaccctatg atgaattctg
     4981 gcgggtaaaa aatacatggg ccgtcatcag ccttgtaccg gccaggccga aaaggccctg
     5041 accgacacga attctggcat cagccggtac tgactccatt cttcatctct ctttcccgaa
     5101 ggatgtgttg gaatacgggt atggtcccac ctcccatgaa ctacctcata ggtatcctgt
     5161 ccacacaaaa aaacctgagt taccttcaga cctacgcatg cagaatgcac tgcatgaggc
     5221 agttgtattt aaaaaagaga gttgccgggt cgcggaaatg tcctgttctt ccttcgcctg
     5281 gaaggatagt attcctggta gggattacct gccccgggag atggcccgca ccgcggcaag
     5341 gaggagatcg atctcctgct ccgtggtata gagggcgagg cttgccctga ccgtaccatc
     5401 cgggagaccg agtgattcca tcagtggctg gcagcaatgg taccctgatc gcacgaggat
     5461 ctcggcctgg tcgtccagct gctgggcgat ctcgtgggga tgcatccctt cgattgtgaa
     5521 cgagaccacg ccgatccggg cgtcaggatc ccgtggggca tacacccgga cgcttacaat
     5581 cccctgcagg ccgtcaatca gccgggtagc aagggtgctc tcgtggcggt ggatccgttc
     5641 cattcccttt gccatgagat actctgccgc aaccccaagg ccgatccccc cggcgatgt
//
LOCUS       DAOOCO010000130         5687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13378_length_5687_cov_5.281250, whole genome shotgun sequence.
ACCESSION   DAOOCO010000130 DAOOCO010000000
VERSION     DAOOCO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13378_length_5687_cov_5.281250"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK154_07700"
     CDS             complement(<1..1087)
                     /locus_tag="PK154_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPW10980.1"
                     /translation="MKKERCDESPPISRAKKKLLQELAREHGTPIFVIDHEKIRQNYR
                     EFRQYMPDVQVYFAVKANSTPSIVKTLYDLGGSFDVASLPEFMIVYENIKSMPEQERL
                     DWIWDKIIYANTIKPIETLQVLDQYKPLVTFDNHEELKKIRQYAPHAGLVLRLRVPNT
                     GSMVELSSKFGADPGEAVDLIEEAFGMDLVVEGLSFHVGSQCTNFENYMQALQLSANI
                     IDEAESRLDRKIRILDIGGGFPVKYHPGVKSLRMLAKKLNAEIKRLFPPDMQILAEPG
                     RFLVANACTLVAKVVGKTIRDGKPCYYINDGVYHTYSGQIFDHCTYPVLSFKPGERQL
                     SAVFGPTCDSFDTITLSAELPELDIGDL"
     gene            complement(1341..2090)
                     /locus_tag="PK154_07705"
     CDS             complement(1341..2090)
                     /locus_tag="PK154_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW10981.1"
                     /translation="MVNSSGEFPLSCRIIALVQERNRLFSYPLDHLAERIRETGFCCI
                     SCGACCTRIRNGHVFLLDRDAAAARSIDPSSLVPAPDPEFCDQNGTFYVSGYSLRTRG
                     DAAGSCWFLRDGRCRIYAQRFMVCRTYPYMLRRVVDEQGSPDWRFIARLYQHGSSGHR
                     IHPEECRRIALETREYENALLMHEITFLEFMRGYLLEEGLYHDPVQYARSLHRAAAGD
                     PVTIMVYYDQGLEEQVVGGGGVGRRSSLSFC"
     gene            2223..2630
                     /locus_tag="PK154_07710"
     CDS             2223..2630
                     /locus_tag="PK154_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW10982.1"
                     /translation="MTAPMPPGQDCLQCGRCCERWGWGQKGIAEDLIPWLTSGRQDIL
                     RHVSILLDDGTKLSGTSLSIADLPRIARIRYWQDPEGKELRYCPFFWRSDDGRSWCRI
                     HDVKPRVCREFTPWNWKNLDYYGSCPACRDKAP"
     gene            complement(2663..3007)
                     /locus_tag="PK154_07715"
     CDS             complement(2663..3007)
                     /locus_tag="PK154_07715"
                     /inference="COORDINATES: protein motif:HMM:NF036688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HPW10983.1"
                     /translation="MAETKNNFWMGTLAGAVIIIILDLLVPFFGPIIGGFIAGYIADR
                     DIMNAGKAGLIAGILAGIVVSLVVFAGLVSHPISGYVPAAGTGFFLMVTIILYLAILA
                     FLGGAIAGAVRK"
     gene            3203..4900
                     /locus_tag="PK154_07720"
     CDS             3203..4900
                     /locus_tag="PK154_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW10984.1"
                     /translation="MKRETKSIALFDDGSRASNAFRLHFQGSPGFSVTRVYSREELEH
                     RMRKTGFDIIISPANSPIHAGAGDPESPRIWLFWSAHAPQRNGPGPRKLSFRFEIETP
                     ETSLSYHSSPEESLGEQFDSLKRLLVRIDAYHREFSRFQADTQILQSIVEDTVTGILY
                     LLRNTIQWTNREAQRILGMSEGELAGRAFSSLFADDRAYRDGLTLLSRNPDPGGWGKS
                     RCSLVRKSGEPVECTLRMRRLNPMNPQKGHLVVIEDDHDRRYLEWAVSTYQEKMAKNE
                     MKYLDILQRMDQVIIRTNPDGVITFWNNRAETTFGYTAEEVSGKNIISVTTDNGSRSA
                     KDMTVLLHDPGSERENALHILENRRKNAEHLWAAWSALTCRDLSGTLAGIVWIGQDIS
                     GGDPSIPPVPGTGHWKSRILQGTDVVEEVFDLIFHAAIQLGRGGREAKKIGTSFVIGD
                     AATVMVHSRQSSINAFEGKDRGQRMIQDRGVIENIKNLALLDGAFVIDGSGFIHASSR
                     HLLADITEIEIPEGFGTRHASVAAMTAITRSIGIVVSESGGTVTVFRDGRLEKQIEP"
     gene            complement(5138..5350)
                     /locus_tag="PK154_07725"
     CDS             complement(5138..5350)
                     /locus_tag="PK154_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10985.1"
                     /translation="MVFAVLVAALGPSDVITPGRIAQKLVFFVPMVLLGGLIGFCASR
                     QKVAWLIFSACCMTCWVIFFLEAGIS"
     gene            complement(5388..5567)
                     /locus_tag="PK154_07730"
     CDS             complement(5388..5567)
                     /locus_tag="PK154_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10986.1"
                     /translation="MTMNSRLLVPLLLSLAFPLVLVVLLHSGISPGSTPYVIGEIALI
                     LLFFSDSPPLRIYYR"
BASE COUNT         1370 a         1563 c         1621 g         1133 t
ORIGIN      
        1 ccaggtcacc gatatcgagt tccgggagct cggccgagag ggtgatcgta tcgaacgaat
       61 cgcaggtcgg tccgaagacc gcggagagct gccgttcccc gggcttgaag gagaggaccg
      121 ggtaggtgca gtggtcaaag atctgaccgg aataggtatg gtacaccccg tcattgatgt
      181 aatagcacgg ctttccatcc cggatagtct ttcccaccac ctttgccacc agcgtgcagg
      241 cattggcaac gaggaatcgc cccggttccg caaggatctg catatcgggg gggaacagcc
      301 gtttgatctc tgcattcagc ttctttgcta acatcctcag ggatttgacc cccggatgat
      361 acttcaccgg gaaccctccc ccaatatcaa ggatccgtat cttccggtcc agccgggatt
      421 cagcttcatc gatgatattg gctgacaact ggagcgcctg catgtaattt tcaaaattgg
      481 tgcactggct gccgacatgg aagctcaggc cttcaacgac caggtccatc ccgaacgcct
      541 cctcgatcag gtccactgct tcgcctggat cagccccgaa ctttgaggag agctccacca
      601 tcgagccggt gtttggcacc cggagccgga gcacgaggcc ggcatgcggg gcatactgcc
      661 ggatcttttt taattcctcg tggttgtcaa acgtcaccag gggcttatac tggtcgagga
      721 cctggagggt ctcgatcggt tttatcgtgt tggcatagat gatcttgtcc cagatccagt
      781 ccagccgctc ctgttccggc atgcttttaa tattctcata gacgatcatg aactcgggga
      841 gggaagcaac atcgaaactg ccccccaggt cataaagggt cttcacaatc gacggggtgg
      901 aatttgcctt gaccgcgaag tagacctgga catcgggcat gtactgcctg aactcccggt
      961 aattctgccg gatcttttcg tggtcgatga caaagatcgg ggtaccatgt tcgcgtgcca
     1021 gttcctgcag cagcttcttt ttagcccggc ttatcggagg agactcatca caccgttcct
     1081 ttttcatttc ctgttcccat cccggtcgat tcttctgtac atgagtgatt cattgcacga
     1141 ataagaattt tgtgaattga gaagggatca cagaatcctt tccgtgcaag gagggctcct
     1201 gaagagatgg gacgattgcc cgggcaaccg gaattgtgaa gatacagggg ctggttcaat
     1261 gtcacccggg aacttctcac acctattcag gctcccagtt ggcaggaaca ggagatattc
     1321 gtcagacgat cggtgatgta tcaacagaag gacagggagc tcctcctccc cacccctccc
     1381 cccccgacca cctgttcttc caggccctgg tcgtaatata ccatgatagt caccggatca
     1441 cctgcggcag cccggtgtaa tgaccgggca tactggaccg gatcgtgata gagcccctct
     1501 tccaaaagat accctcgcat gaactcaagg aaggttatct catgcatgag cagggcattc
     1561 tcatactccc ttgtttccag ggcgatacgg cggcactctt ccgggtgtat ccggtggcct
     1621 gaacttccat gctgatacaa cctcgcgata aaacgccagt cagggcttcc ctgctcatcc
     1681 acaaccctcc ggagcatgta tgggtacgtt ctgcagacca tgaatcgttg ggcgtagatc
     1741 cggcacctgc catcccgcag gaaccagcat gaaccggctg cgtctcccct ggtccggagc
     1801 gagtaccctg aaacatagaa ggtgccgttc tggtcacaga actccgggtc aggagcagga
     1861 acaagcgagg agggatcgat tgaccgggca gctgctgcat cccggtcgag caggaagaca
     1921 tggccattcc ggatccgcgt acagcacgct ccgcacgaga tacagcaaaa tcctgtctcc
     1981 cgtattcgtt ctgcgaggtg gtccagggga taggagaaga gacggtttcg ctcctggacg
     2041 agcgcaatga tccggcagga gagcgggaat tcgcctgaag agttcaccat gccgataaga
     2101 gggagtgcgg ctgcgggatg atatgctttt cccgctgagg gaatccggag atctgttcca
     2161 ggaagagaag aacgataggt tttcagacca cgggaaagat actagggata tcacgatgcg
     2221 atatgactgc acccatgcca ccaggccagg actgcctcca atgcgggcgc tgctgtgaac
     2281 gatggggatg gggccagaaa gggatcgcag aagacctcat cccctggctc acgtccggcc
     2341 ggcaggacat cctccggcat gtcagcatcc ttcttgacga cgggacaaag ctcagcggca
     2401 cctccctttc gattgccgac ctgccaagga tagcccggat ccggtactgg caggaccctg
     2461 aggggaagga actgcggtac tgcccgttct tctggcgatc agacgatggg cgatcctggt
     2521 gcaggatcca tgatgtgaag ccacgggtat gccgggaatt cactccctgg aactggaaga
     2581 accttgacta ttacgggagc tgccctgcct gcagggacaa ggcgccctag ggagagatct
     2641 ctcttgaaag ggggaagtga gattatttcc tgacagcccc tgcaatggca ccaccaagga
     2701 atgccaggat tgcaaggtac aggatgatgg tcaccatgag gaaaaatccc gtgccggcag
     2761 cagggacata ccctgatata gggtgagaca ccagtcctgc aaagacgacc agggagacga
     2821 caatccctgc caggatcccg gcaatcagcc cggccttccc ggcattcata atatcgcggt
     2881 ctgctatgta cccggcaata aaccccccta tgatggggcc aaagaaggga acgagcagat
     2941 cgagaatgat gatgatgacc gcccctgcga gggtgcccat ccagaagttg ttcttcgttt
     3001 cagccatacc agtcttcacc tggacagtga tagggctccc atgatataaa ggtgggggag
     3061 aaaagaccgc ggcactgctc ctttcagaga ccgaacaagg ggggtatacg aacgggaaca
     3121 gccaaaaaac tcgaatattt catccatttc ctccaaaaat tatttatctg tctgcttttc
     3181 tattggatac tgttcatttc gcatgaaacg ggaaacgaaa tccattgctc tgtttgatga
     3241 tggctccagg gcatccaacg cgttcaggct ccatttccag ggcagcccgg gattctcggt
     3301 gacccgggtg tactcaaggg aggaactgga acacaggatg cgaaagaccg ggtttgacat
     3361 catcatctcc cctgccaact cccccattca tgcaggtgcc ggagatccgg agagtccaag
     3421 gatttggctc ttctggagtg cccatgcgcc gcagaggaac ggtcctggtc cgagaaaact
     3481 ctccttcaga ttcgagatag aaaccccgga gacctcgctc tcgtatcatt cctcccctga
     3541 ggagtccctc ggggagcagt tcgattccct gaagcggctg ctcgtccgca tcgatgcgta
     3601 ccacagggag ttctcccggt tccaggccga cacccagata ctccagtcca tcgttgagga
     3661 taccgtgacc ggcatcctct acctcctccg gaatacgatc cagtggacaa accgggaggc
     3721 acaacgtatc ctcgggatga gcgagggcga actggccggg agggcattct cctcgctttt
     3781 tgccgatgac agggcgtacc gcgatggtct taccctgctt tcgcggaacc ccgaccctgg
     3841 gggatgggga aaatcccgct gttccctcgt ccggaagtcc ggggagcccg tggagtgcac
     3901 cctccgtatg cgacggctga acccgatgaa tcctcaaaaa gggcacctgg tcgtgatcga
     3961 agacgaccat gaccggaggt acctggagtg ggcagtgagc acctaccagg agaagatggc
     4021 caaaaacgag atgaaatacc tggacatcct ccagcgaatg gaccaggtga tcatcaggac
     4081 caatccggac ggggtgatca ccttctggaa caaccgggcg gaaacaacct tcggctatac
     4141 agccgaggag gtgagcggga agaacatcat cagtgtcact actgacaacg gttcgcggtc
     4201 ggcaaaggac atgaccgtcc tcctccatga tcccggatcc gaacgggaga atgccctgca
     4261 tatcctggag aaccggagaa agaacgcaga gcacctctgg gctgcctgga gtgccctcac
     4321 ctgccgtgac ctttccggaa ccctggcagg catcgtctgg ataggacagg atatcagtgg
     4381 cggtgatccc tcaatccctc cggtaccggg gacaggacac tggaaatccc ggatccttca
     4441 ggggactgat gtcgtcgaag aggtctttga tctcatcttc catgcggcaa tccagctcgg
     4501 gaggggaggg agagaagcaa aaaaaatagg gacctcgttt gtcatcgggg atgcagcgac
     4561 agtgatggtg cactcacggc agtcctctat caatgcattc gagggaaagg atcgtggcca
     4621 gcggatgatc caggatcgcg gggtcatcga gaatataaag aatctcgcgc tgcttgacgg
     4681 tgctttcgtg atcgacggat ccggatttat tcatgcctcc tcccggcacc tgctcgccga
     4741 catcacggag atcgagatcc cggaagggtt cggaacccgc catgcctcgg tggccgcgat
     4801 gaccgcgatc actcggtcga ttgggatcgt cgtctcggag agtgggggga cggtaacggt
     4861 gttcagggat ggcagactgg agaagcagat cgaaccatga cctccggcgg gtcagccctc
     4921 catccgagaa ggggaagacc tccataataa ggagcagata aaaagcagga tacgccctgg
     4981 acgccaggaa cctgccatga gctgacagaa aaacagggag ggctatctga atccgtggat
     5041 ttcctggcgc cacaaacgaa ctgccaggat cccttcctct gaaatgtagc cggcaaaccc
     5101 ttgctgcagg tctggcagaa aactacgggg agtttcctca actgattcct gcctccagaa
     5161 aaaagatcac ccagcaggtc atgcagcagg ctgaaaaaat gagccaggcg accttctgcc
     5221 gggaagcaca aaaccctatg agacccccga gaaggaccat cggtacgaaa aagaccagct
     5281 tctgggcgat ccggccgggt gtgatgacat cggaaggacc cagtgcagcg acaaggaccg
     5341 caaagaccat gagagggacg gtcccggaga tgacggaact acgtgcatca ccggtaataa
     5401 atccgtagag gaggggaatc agagaagaac aggaggatca gcgcgatctc cccgatcaca
     5461 tatggagtgc ttccaggaga tattccggag tgtaacagaa caacaagaac gagggggaat
     5521 gcaagagaga gaaggagcgg aacgaggaga cggctattca tggtcatgct accaccatca
     5581 atcagatagc taccgtggtc gttgaaccga taccacgagg ttcacctgtt caatcacgta
     5641 tacacctgat tgatgggggg gcagatatca tacaggagga cctgtgc
//
LOCUS       DAOOCO010000131         5677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13402_length_5677_cov_5.164532, whole genome shotgun sequence.
ACCESSION   DAOOCO010000131 DAOOCO010000000
VERSION     DAOOCO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13402_length_5677_cov_5.164532"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..703
                     /locus_tag="PK154_07735"
     CDS             365..703
                     /locus_tag="PK154_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPW10987.1"
                     /translation="MGSYFRGDVLLAPLCISKGREAKVRPVVVLGSSGRSGLFVIAVS
                     STLPYEGPVVPISIDDFSEGGLDLVAESYLVCSRICRIRVSDVIGKKGRLTREMIQNL
                     PVPERGDTIP"
     gene            743..1462
                     /locus_tag="PK154_07740"
     CDS             743..1462
                     /locus_tag="PK154_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPW10988.1"
                     /translation="MKGSVEGFLVVCWSRSPVPASTIRRINGCTEKSCIMASYCMIST
                     AGSWKFPAVLGIIGLLIGAIFLFFPHLSLTLVIYLFAAIALIAGILLLAAAAFLAKGS
                     GGFFAVPLVLGVIAVILAVYSFLNPESTATFIAIVIGAGCIIMGLIGAFTALFQPSPA
                     IRRALLAVGGFVLAGLGIIILLFLQRSVLLIFQLLGAFLLIAGAVALIGAAVIVLRRR
                     PNEPGRRPDDPSVIDAEFREG"
     gene            complement(1725..2264)
                     /locus_tag="PK154_07745"
     CDS             complement(1725..2264)
                     /locus_tag="PK154_07745"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPW10989.1"
                     /translation="MVQKKSILLEVQIIKILISLLLIGIGVPLLLGILSGKSFASVLS
                     FIGSTAALQALAAPVGVILDFDPWLVLAIMTAFAFGICLGIWEALQTFALTSDRVAGW
                     ISRVEEKMQEHQSLHRYGPVSCILIAWIPGIGLYGTPAIAWILRWKRLPSVLFTVIGF
                     FLASLLMIVLAEGASSILQ"
     gene            2520..3317
                     /locus_tag="PK154_07750"
     CDS             2520..3317
                     /locus_tag="PK154_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPW10990.1"
                     /translation="MSLKRSLAGIIPEDKISLLSDRYEVIGDLAILDIPAGLEAYQDD
                     IALALISKRKNIRTVLRKSGKIEGAGRLAAFEVLIGSGTITEHHEGGYRYRMDVAKVF
                     FNSRLGTERMRVAAQVRPGERVLVPFCGVGPFVVPAAARGASVVAVDQNPDAIRWLFQ
                     NLVLNRVFGQVRAVRDDAFRIPRVVEPGFDRVIVPTPYGSDRILAILPGMTKKGGNIH
                     FYTFKPEDEIGSLMESFGDNGLTCTASHKCGSVAPGICRYVFDLVKG"
     gene            complement(3505..4581)
                     /locus_tag="PK154_07755"
     CDS             complement(3505..4581)
                     /locus_tag="PK154_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10991.1"
                     /translation="MADEGCREVRIAIAGAGIAGTYISHLLAKRGIETDLYDGRDQGT
                     PCRYRSCGWGAPSGIGHYLAAVDLDLEGYLMDSMSSMEFDGLIASTPLCTINKPRLLR
                     DLAAGLALHHHPLDRHDPGDYDIIVDATGICRAFLSPCRSDLVLPTLQHRMKVEPVGD
                     APLIAGVYGNRIPGLGYLWIFPLGNGEYHIGVGGIRLDQHERILEQYYEANAHRYTFT
                     PVCSCRGNVRVASPYYSQPFSLPQGGMDGTSPRIIGVGESIGTVAPFTGEGIIYSLEC
                     ARILAACWPDQDRYKRTVLAQFAWMRRERETLEYLLLEGKETGPRLKDRWRFYLNARR
                     SGIRLPLAEAFRRMGSLSRWVENP"
     gene            complement(4588..4944)
                     /locus_tag="PK154_07760"
     CDS             complement(4588..4944)
                     /locus_tag="PK154_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10992.1"
                     /translation="MTFITDPSPYNREGSGFAVHNLADPARTDLRETPLQGMDVSCSG
                     KNGYVMKSLEARMTPSGREGKIIFHYTDFLLEKDRDKTENGVSKIFWRTSPIILVSPD
                     YGSQCILLSSPIEDTQ"
     gene            4934..5608
                     /locus_tag="PK154_07765"
     CDS             4934..5608
                     /locus_tag="PK154_07765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW10993.1"
                     /translation="MKVIGIIASPRGKASNTLQLMNGVLKGAREEGAATELIDIYSLD
                     IGYCTACGNCYVSGDCTLFDDFPDLFDTLMDADGIVLGAPNYIDSIPAPLKAMFDRMA
                     DAIHCQMFSGKYGCSVCTAGGSNHGPVVEYMNHALSSLGAITVGGVGVAMKDGEEALS
                     TGIRDSVELGRKLVSSIRGEYSYPEQEAELLQRRDYFRQLVLWNKDKWTHEYDWYRQM
                     GWLSPE"
BASE COUNT         1188 a         1561 c         1629 g         1299 t
ORIGIN      
        1 cggccggtag agtcgtttca tgaaagacat ttgggggatg ccacgtctta atggttacac
       61 tcgaacattt cgggccttcc cccgaaggaa ggggatacct tgtgcgcata ttgccggtgc
      121 atcaatcccg atcattcccc ggagcgctga tctcgatacc tcatacctct tttaccatgc
      181 tccgatatga gacgattcag ggagccggtt gatcgtaggg gtgcctcccc ggatgggcaa
      241 cagaaggcat aacccctggc aagtgcgtat ccatcagtac tcttatgcca gtctggccga
      301 tccttccctc ctttggaaca taccgtatgg cttatacccg ccgttaccaa acgtagctct
      361 actcatggga tcctatttcc gcggtgatgt cctcctcgct ccactctgca tctcgaaagg
      421 gagggaagcg aaggtccggc cggtggtggt cctcggttca tccggacgtt ccgggctgtt
      481 cgttatcgcg gtatcgagca ccctccctta cgaaggacca gtggtcccga tctcgatcga
      541 tgacttttcc gaaggggggc tcgacctggt ggcggagagt tacctcgtct gctcccgtat
      601 ctgccggatc agggtctctg atgtcatcgg gaagaaaggc aggctcacaa gagagatgat
      661 acaaaacctc cccgttccgg aaaggggcga taccatccca taggcatcac gaatttcccg
      721 ggggagtcct cccgaacctt ccatgaaagg gagcgttgaa ggattcctgg tggtatgctg
      781 gtcccggtcg cctgttcctg cctccactat ccgcaggatt aatggctgta cagaaaaatc
      841 ctgtatcatg gcatcatact gcatgatcag cacggccgga tcatggaagt ttcccgctgt
      901 gcttgggatc atcgggcttc tgatcggggc gatattcctg ttcttccccc acttgtcact
      961 caccctcgtg atttatctct ttgccgcgat tgcgctgatt gccggcatcc tcctccttgc
     1021 tgctgctgcc ttccttgcga agggaagtgg tggatttttt gctgtccctc tcgtgcttgg
     1081 tgtcattgcc gttatccttg ccgtatattc atttctcaat cccgagagca ccgcgacatt
     1141 cattgcgatc gtgatcgggg caggttgcat catcatggga ttgatcgggg ccttcactgc
     1201 tctcttccag ccttcccctg cgatcaggag ggccctcctt gcggttggtg gttttgtcct
     1261 tgccgggcta ggcatcatca tcctcctgtt cctccagcgc tccgtcctcc tgatattcca
     1321 gctcctgggg gcatttctcc tgattgccgg ggcggttgcc ctcatcgggg cagctgttat
     1381 agtgctccga aggagaccca atgaacctgg gaggagaccc gacgaccctt ccgtgattga
     1441 tgcggagttt cgggaagggt aagagcggtc gtattgcatc acgatctctc ccgttccaat
     1501 gacactgccg acggtagttc catctttgat gatcgaccca tggcgttcac cggtggttac
     1561 tatgaaacta ctaccgggat ttacatcgtt gttttgtgta gtccatgagt gattcatgta
     1621 attcttttgg aaaaaaatgt gaggatatcc cctcgcctgg attttcaggg agttccgggg
     1681 agccgggggt cctttgctgc agcccctcgg tggtatcact ggtatcactg aaggatactg
     1741 gatgctcctt cggcaaggac gatcatgaga agcgatgcaa ggaagaaccc aatgaccgtg
     1801 aagagaacag aggggaggcg cttccaccgg aggatccagg caattgccgg cgttccatac
     1861 aggccgatcc cggggatcca tgcgatcagg atgcaggata cagggccata ccggtgcagg
     1921 gactggtgct cctgcatctt ctcctcgacc ctgctgatcc agcccgctac ccgatcagag
     1981 gtgagagcga aggtctggag cgcttcccag atgcccaggc agataccgaa tgcaaaggcg
     2041 gtcatgatcg cgagtaccag ccagggatca aaatcgagga tcacccccac cggtgcagca
     2101 agtgcctgaa gagcagccgt tgatccgata aaagagagaa cagaggcgaa agatttgccg
     2161 gataaaatgc cgaggagcag ggggacgccg atgccgatca gtagcaggga aatgagaatt
     2221 tttatgatct gcacctcgag aaggatgctt tttttctgca ccatacctgg tcaaaggttg
     2281 cccctgggga gataaataac ccgctcccag gggcattgat gaaaacggcc atcccctggt
     2341 cacctgattc tgacaggcat caggcctcac cagtgtcata accagcggat ttctcctgct
     2401 gttgcggggt gtccggtgtg aagggcgggc tttggagagc ggttcaggag atccgttttt
     2461 catcgtcgct gaaacgacgg agtattttaa tagttcctga cgtaatgtat gatcccagta
     2521 tgagcctgaa aaggagcctt gccgggatca ttcccgaaga caagatctcc ctcctctcgg
     2581 accggtacga ggtgatcggg gatctggcga tcctcgacat tcccgcgggg cttgaggcat
     2641 accaggacga catcgccctt gctctcatct caaaacggaa gaatatccgg acggtactga
     2701 ggaaatccgg aaaaatcgaa ggggccgggc ggcttgcggc ctttgaggtc ctgatcggat
     2761 ccggtacaat aaccgagcac cacgagggcg gataccggta tcggatggat gtggcaaaag
     2821 tctttttcaa cagccgcctt gggacggagc ggatgagggt ggcagcccag gtcaggcccg
     2881 gcgagagggt gcttgttccg ttctgtggtg tcggcccttt tgtggtcccg gctgcagcac
     2941 ggggagcaag cgtggtcgcg gtggaccaga atcctgatgc gatccggtgg ctattccaga
     3001 accttgtgct gaacagggta tttggccagg tccgggcagt ccgtgacgat gcgttccgaa
     3061 ttccccgcgt tgttgaacca gggtttgacc gggtgatcgt gccaactccc tatggtagtg
     3121 acaggatcct tgcgatcctt cccggaatga ccaaaaaggg cggaaatatc catttctaca
     3181 ccttcaagcc ggaggatgag atcggatccc tcatggaatc attcggtgat aatggcctca
     3241 cctgcactgc ctcccacaaa tgcggatccg tggccccggg gatctgcagg tatgtctttg
     3301 acctggtcaa ggggtgatac tgcagacctc ccacgcttca ggcaggtacg gatccaatcc
     3361 cgcaggaatc cgaagttccc ggggtagtcc gatctattgt tatggtgtga gcgattgaaa
     3421 tccatgcatt catagaccgc acttcttccg gctgcagggt gtccccgatg aataagcgat
     3481 cggatatgcg gttcttttcc cggttcacgg attttccacc caccgtgaca ggcttcccat
     3541 gcggcggaac gcctcggcca gggggagcct tatccctgag cgcctggcat tcaggtagaa
     3601 ccgccaccgg tctttaagcc gtggcccggt ctccttcccc tccaagagga ggtactccag
     3661 ggtctcccgt tcccttctca tccatgcaaa ctgtgcaagg acggttctct tataccgatc
     3721 ctggtccggc cagcacgctg caaggatccg ggcacattcc agcgagtaga tgataccttc
     3781 gccggtgaag ggtgccaccg tcccgatcga ttctcccaca ccaatgattc ttggggatgt
     3841 tccatccatc cccccctgcg gcagggagaa tggttgtgag taataggggg aggccaccct
     3901 gacgttcccc ctgcagctgc agaccggggt aaaggtatac cggtgagcgt tcgcttcgta
     3961 atactgctca aggatgcgct cgtgctggtc gaggcggatt ccccccacgc cgatgtgata
     4021 ctcgccgttt ccgagcggga aaatccagag gtaaccgagc cccgggatcc ggttgccata
     4081 aacccctgcg atcagtgggg cgtctcccac cggttcgacc ttcatcctgt gctgcagcgt
     4141 gggaaggacc agatctgatc ggcaggggga caggaatgcc cggcagatcc ccgttgcatc
     4201 cacgataatg tcatagtcac ccggatcatg acgatccagg gggtgatgat gcaaggcgag
     4261 cccagccgcg agatcccgga gaaggcgggg cttgttgatg gtgcagagcg gggtactggc
     4321 gatgagtcca tcaaactcca tggaggacat cgaatccatg aggtacccct ccaggtcgag
     4381 atcaacggca gcaaggtagt gcccgatgcc ggacggggct ccccatccgc atgaccggta
     4441 ccggcatggc gttccctgat ccctgccatc atagagatcg gtttcgattc ctctctttgc
     4501 caggagatgg gaaatatagg tgcctgcaat tcctgcaccc gcgatcgcga tcctcacctc
     4561 cctgcacccc tcatcagcca cacctgttca ttgggtatcc tcaatcgggg aggacagtaa
     4621 aatgcactgg gaaccatagt ccggtgatac tagtatgata gggctcgtcc tccaaaaaat
     4681 ctttgatacc ccattttcgg tcttgtccct gtctttttcc aaaagaaagt cagtataatg
     4741 aaaaattatc ttcccctccc tgccggatgg tgtcatccgg gcttcaaggg atttcatcac
     4801 gtacccattt ttcccggaac atgacacgtc catcccctgc aacggtgttt cccgtagatc
     4861 cgttctggcc ggatcggcaa ggttatggac cgcaaatcca gatccttccc tattataagg
     4921 agaaggatca gttatgaagg tcattgggat tattgccagc ccgcggggaa aagcgagtaa
     4981 taccctgcag cttatgaacg gggtgctgaa gggtgcacgg gaggaagggg ccgcaaccga
     5041 actgatcgat atctactcgt tggatatcgg gtactgcacg gcatgcggga actgttacgt
     5101 cagtggtgac tgcacgctgt ttgatgattt tcctgatctc ttcgataccc tgatggatgc
     5161 cgatgggatt gtgcttgggg cgccgaacta catcgactcg atccctgccc cgctcaaagc
     5221 catgtttgac cggatggccg atgccatcca ttgccagatg ttctccggga agtacggctg
     5281 ctcagtctgc acggcaggcg gaagcaacca tggccctgtt gtggagtaca tgaaccatgc
     5341 cctttcttcg ctcggggcca tcacggtcgg gggagtggga gtggccatga aggatgggga
     5401 ggaggcgttg tcaaccggga tccgggactc ggttgagttg ggcaggaaac tcgtcagttc
     5461 catccgcgga gagtacagct atccggagca ggaagcggaa ctcctgcagc ggcgggacta
     5521 cttcaggcag ctcgtgttgt ggaacaagga caagtggacc catgaatacg actggtacag
     5581 gcagatgggc tggctctccc ctgagtgatc agccgagatc gcaccacgat aaaagaggaa
     5641 ggcgtgcaag gagggagacg cacaggtcct cctgtat
//
LOCUS       DAOOCO010000132         5628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13555_length_5628_cov_5.004306, whole genome shotgun sequence.
ACCESSION   DAOOCO010000132 DAOOCO010000000
VERSION     DAOOCO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13555_length_5628_cov_5.004306"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK154_07770"
     CDS             <1..388
                     /locus_tag="PK154_07770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW10994.1"
                     /translation="AAFSVQEVPDITLASKNIMGVVVPEIESSKVKKSLVDRGYGLLG
                     TTSAIDEAAASYEDLVEAIIESAEIETTMKKLLDEIESTKRRVNALEFKVIPELTEAR
                     DFIKMRLDEMEREELFRLKKIKARNT"
     gene            402..1619
                     /locus_tag="PK154_07775"
     CDS             402..1619
                     /locus_tag="PK154_07775"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPW10995.1"
                     /translation="MAPIGLLGETPCAGKTVLLRLDLNSPIDPSTHSILDDKRFREHI
                     PTIQALRESRLVIITHQSRPGKKDFTTLEVHAEKLEQLAKRPVKYIDDIFGHYARESV
                     RSLKAGDILMLENVRFNAEENLTMKPEEAARCHLVRGLAALGDLFVNDAFGTAHRSQP
                     TIVGLPVAMKSVAGLLMEKEVLTLSKVFSNAPRPVSVVLGGTKVDDSIAVAEHMLKDH
                     IADQVIVIGVVANVFLLAMGYDIGRPSAQLIQQLKYEKEIGKAKEILREFGDRIIVPE
                     WVAVREGGARTEYPVSAIPSDAPVLDLGMDSVLRLSDRVRESGTVVFNGPAGVFEDRD
                     FATGTYELLKAAAKTPFSIIGGGHTAAVVEQLGIERDFSHISTGGGACIEFLTGKKLP
                     AVEALERSKELFG"
     gene            complement(1631..2077)
                     /locus_tag="PK154_07780"
     CDS             complement(1631..2077)
                     /locus_tag="PK154_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW10996.1"
                     /translation="MQGIVITASLVFGAVIGLILSFVLLKFGEIIDRYPSGDEESRDK
                     NWGSPIPFTRISVLLFWLFFIIGGWMIFQQSKVIFVGGIALLIGLLLFLATAVVFSLA
                     VLNQMSAKKDEKGISSLLSGRKSSSWFPRMSFHGSGYYPRKSSRKK"
     gene            2580..4277
                     /gene="ade"
                     /locus_tag="PK154_07785"
     CDS             2580..4277
                     /gene="ade"
                     /locus_tag="PK154_07785"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPW10997.1"
                     /translation="MIHDENYRGERGSIPAGDRTIPADIRFLNAEVFNPFTGEWQDSD
                     IAVKDGRVIGHGAYRATTEIDLKGRRVVPGLIDAHVHIESSLLSPPEFARLASLHGTT
                     TIIADPHEIANVLGAEGINFMLRWRGRIPLDLFIMVPSCVPATPLDTGGAVLNARDLA
                     PYSGREGVLGLGEVMNVPGVLSGDPDVMDKIHLFRIVDGHAPLLSGPDLDQYIAAGIQ
                     SDHECTSFGEAEEKLEKGMYLFIREGSTEKNIREIIPLVSWKNVSRCSFATDDRHADL
                     LLAEGHIDDCIRKAVGSGLEREIALRMATLSPAERFSLHDRGALSPGRRADFCVICEG
                     NEFRVEKTFRGGELVVPGQRIPCPPIDGQFRCRTPGVADIHLDGRGPALVIGLVPRQI
                     MTKRLSLDVDSTKIPDPDRDILKVVVCSRYEKGKVATGLVQGFGITSGAIAGSVSHDS
                     HNIVATGSGDADIIRAISLVIRNSGGLAVVSGDESTVLPLECAGLMSVRPYEEVSELL
                     GRLNRHAREIGGIEDSFMYLSFLALSVIPHLRITDRGLFDTDSFSYVPVFSEDESLT"
     gene            complement(4283..5053)
                     /locus_tag="PK154_07790"
     CDS             complement(4283..5053)
                     /locus_tag="PK154_07790"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HPW10998.1"
                     /translation="MTASHVPERYPEIDIARGIAVVMMVLFHIAFDLSFLGIAGIPVS
                     SLPWRAFAMATAALFLLLVGISLSISAGYAKTRLSRSDFILKYLKRGAGIFAIGIGIS
                     IVTWIILPGAFIVFGILHLIGLSIALSPLYTGYSWQNLLAGLAIILVSPVVAAMRGDG
                     WLVWLGIHPPAFYSIDYTPVFPWLGVVLIGVGLGAILYPRAARRWDHEVPTLPRSPLR
                     FLGRHSLAIYLVHQPIILGILFITFPDAFSAFVPHLSG"
     gene            5322..>5628
                     /locus_tag="PK154_07795"
     CDS             5322..>5628
                     /locus_tag="PK154_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPW10999.1"
                     /translation="MIDNLLEGNKKFREGFFKENRAVYDELSKGQSPSVLWIGCSDSR
                     LQTGHITQTMPGTLFIQRNIGNVAPLHDWNFATVLEYAIRHLKVKDIVICGHSDCGAI
                     "
BASE COUNT         1335 a         1511 c         1637 g         1145 t
ORIGIN      
        1 cgcagccttc tcggtccagg aggtcccgga tatcacgctt gcaagcaaga acatcatggg
       61 ggtcgttgtc ccggagatcg agtcatcgaa ggtgaaaaag agcctcgttg accggggata
      121 tggtctcctc gggaccacct ccgccatcga tgaagccgcc gcctcctatg aagacctggt
      181 ggaggcgatc atcgagagtg ccgagatcga gaccaccatg aagaagctcc tcgacgagat
      241 cgagtccacg aagcggaggg taaacgccct ggagttcaag gtgataccgg agctgaccga
      301 agcacgggac ttcatcaaga tgcggctcga cgagatggaa cgcgaggagc tcttcaggct
      361 gaagaagatc aaggcccgaa atacctgacg gaggaaggga tatggctcct atcggtctgt
      421 tgggagagac cccctgtgcg gggaagaccg tgctcctgcg gcttgacctg aactccccca
      481 tcgatccctc cacccactct atcctcgatg acaagcggtt ccgggagcac attccgacca
      541 ttcaggcgct ccgggagagc cgtctggtga tcatcaccca ccagagccgg ccggggaaga
      601 aagattttac caccctcgaa gtgcacgcag agaaactcga gcagctggca aaacggccgg
      661 tcaaatatat cgacgatata ttcgggcatt atgcccggga gtcggtcaga tccctgaagg
      721 ccggcgatat cctgatgctt gagaatgtcc gtttcaatgc cgaggagaac ctgacgatga
      781 aaccggagga ggcagcccgg tgccatcttg tcagggggct cgcggccctg ggcgatctct
      841 ttgtcaacga tgcatttggg actgctcacc ggtcacagcc gaccatagtc ggccttcccg
      901 ttgccatgaa atcggtagcc gggctcctca tggagaaaga ggtactgacg ctctcgaagg
      961 tcttttcgaa tgccccccgc ccggtatcag tggtcctggg ggggacgaag gttgatgact
     1021 cgatagcagt cgcagagcac atgctcaaag accatatcgc cgaccaggtg atcgtcatcg
     1081 gcgtggtcgc aaacgtcttc ctcctggcga tgggatatga catcggccgg ccatctgccc
     1141 agctgatcca gcagctcaag tacgagaagg agatcgggaa agcaaaggag atcctgcgcg
     1201 agttcggaga caggattatt gtgccggaat gggttgccgt gagagaggga ggagcgcgaa
     1261 ccgagtaccc ggtcagtgcc attccgtcag atgcaccagt cctcgacctt ggtatggact
     1321 ccgtcctccg gctgtccgat cgtgtccgtg agtcaggcac cgttgtcttc aacggaccgg
     1381 caggggtctt cgaggaccgg gattttgcca cgggaaccta cgagctgctg aaagcggccg
     1441 caaagacacc gttctcgatc atcgggggag ggcacacggc agcagttgtc gagcagctcg
     1501 ggatcgagag ggacttttcg catatctcca ccggtggcgg agcctgtatc gaattcctga
     1561 caggaaaaaa gctcccggca gtcgaagcgc tcgaacgttc aaaagagttg tttggctgat
     1621 ccgcgatcct ttacttttta cgggatgatt ttcgaggata gtatccacta ccatgaaagc
     1681 tcatcctggg gaaccatgag gagctctttc tccctgacaa gagggaagat atcccctttt
     1741 catccttttt tgcgctcatc tggttgagaa ccgcaagtga aaacaccacc gcggtggcca
     1801 ggaagagcag aaggccgata agcaaggcaa tcccccccac gaaaatcacc ttggactgct
     1861 ggaatatcat ccatcctcca atgatgaaaa aaagccagaa caggagcact gagatccttg
     1921 tgaatgggat agggctcccc cagtttttat cccggctctc ctcgtctccc gagggatagc
     1981 ggtcgataat ttctccgaat ttcaggagga caaacgagag aataagacca attactgcac
     2041 cgaatacaag actggcggtg atgacaattc cctgcatagc tcaagcgtat ctgagttatg
     2101 tgacggagtt ttagataaat tattcctgtt tctgatggag atgaggctcc tgggaagaga
     2161 gggatcgata ataaaattac atgaaggtat ttgagacaca cacaaaatta aaaatgccgg
     2221 aggaattttc atatttaata gaatgtccac acgaatcgcc tcctggctgt ttcatggtgg
     2281 aacgccgttg agatggtatc ggcataataa tgaggcaatg accgcctccg ggacacctgt
     2341 tctgggtggc ggtgtgcatc acttggtgag gatacggtac gaaaagatgc gaaaagcccg
     2401 tacaacctat atctggtatc aaaggtacct acctcagcga gagaagcgca ggaacacccg
     2461 gtttttttat cccggggaaa aaggaacggt ccatacaagg ccaggtgttt ggagtgtcca
     2521 tgacccccca aatgcgatct catttcgaac aacccttcac cagctgagag gaacccgcca
     2581 tgatccatga tgagaactac aggggagaga ggggatcgat cccggcagga gaccgtacta
     2641 ttcccgcaga tattcggttc ctgaacgctg aagtcttcaa cccgttcacc ggtgaatggc
     2701 aggattccga tattgccgtg aaggacgggc gggttatcgg ccatggtgct tacagggcaa
     2761 ccacggagat cgacctgaaa gggcgccggg tggtccccgg gttgatcgat gcccatgtcc
     2821 atatcgagag ctcgctcctc tcccccccgg aatttgcccg tctcgcatcc ctgcacggga
     2881 caacgacaat catcgccgat ccgcacgaga tagccaatgt gctcggggct gaggggataa
     2941 acttcatgct gcgctggaga gggcgaattc ccctcgatct cttcatcatg gttccttcat
     3001 gcgtcccggc cactcccctg gataccgggg gagctgtgct caacgccagg gaccttgcac
     3061 cctactcggg aagggagggg gttctcggcc tgggtgaagt gatgaacgtc ccgggggtcc
     3121 tgtcgggaga tcctgatgtc atggataaga ttcacttgtt caggatcgtt gacggccatg
     3181 ctcctctcct ttccgggcct gatcttgacc agtacatcgc tgccgggata cagagcgatc
     3241 acgagtgtac gtcattcggt gaggcggaag agaagctgga gaaggggatg tacctcttca
     3301 tcagggaggg atcgaccgag aagaacatcc gggagatcat ccccctggtc tcatggaaaa
     3361 atgtatcccg gtgctcgttt gccaccgatg accgccacgc agacctgctc cttgcagagg
     3421 gtcatatcga tgactgtatc cggaaggcag ttggatcagg gcttgaaagg gagattgcac
     3481 tcaggatggc gacactcagc ccggcagaac ggttctcgct tcacgatcga ggcgccctct
     3541 cacccgggag aagagccgat ttctgcgtca tctgcgaggg gaatgagttc cgggtggaaa
     3601 agaccttcag gggcggggag ctggtggtcc ctggtcagag gataccctgc ccccctatcg
     3661 atggacaatt ccggtgcagg acgccgggcg ttgccgatat tcaccttgac ggccgggggc
     3721 ctgccctggt catcggcctt gtcccccggc agatcatgac aaaaaggctc tctcttgacg
     3781 tcgattcaac aaaaataccc gaccctgacc gggatatcct gaaggtcgtg gtatgcagcc
     3841 ggtacgagaa ggggaaggtg gcgaccgggc ttgtgcaggg cttcgggatc acgtcggggg
     3901 cgatcgccgg gagtgtctcc catgactccc ataacattgt tgcaaccggg tcaggggatg
     3961 ccgatatcat ccgtgcaatc tctcttgtca tcaggaactc gggtggactc gctgtggtgt
     4021 ctggtgatga atcgactgtt cttcccctgg aatgtgccgg cctgatgtcg gtccggccgt
     4081 atgaagaggt cagtgagctg ctcggacgcc tgaatcgtca cgccagggag atcggcggga
     4141 tcgaagattc gtttatgtac ctctcgtttc ttgccctctc cgtgattccc catctgagga
     4201 tcacggatcg cggcctgttc gataccgact cgttttccta tgttcccgta ttttccgagg
     4261 atgaatccct tacctgaggg tatcagccgg aaagatgggg gacaaaagcg ctgaaagcgt
     4321 cgggaaacgt gataaacagg atacccagga tgatcggctg gtgaacgaga tagattgcca
     4381 gggaatggcg gccaagaaac cgcaggggtg aacgtggaag cgttggcacc tcatgatccc
     4441 atctcctggc ggcgcgcgga tagaggatcg cacccagacc gactccaatc agaaccaccc
     4501 cgagccatgg gaacaccggg gtataatcga tgctgtagaa ggccggggga tgaatgccca
     4561 gccagacgag ccagccatct ccccgcatcg ctgccaccac cgggctcaca agaatgatcg
     4621 caaggcctgc gaggagattc tgccacgaat atccggtata gaggggactc agtgcgatgg
     4681 aaagcccgat caggtgaagg atgccaaaca cgatgaatgc gcccggcaga atgatccagg
     4741 tgactatact gatcccaatc cctattgcaa agatacccgc accccgtttc agatacttca
     4801 ggatgaagtc agaccgtgag agtcgcgtct ttgcatagcc agcgctgatg gagagggaga
     4861 ttccgacaag gaggaggaag agcgcagcgg tagccatggc aaacgccctc catgggagtg
     4921 aactcacggg tatccccgca atgccaagaa acgagaggtc aaaggcgatg tggaatagca
     4981 ccatcatcac caccgcaatc ccccgtgcaa tgtcaatctc cgggtatcgc tccggcacat
     5041 ggctggcagt catgaagatg catccctctt cattgtggtt cgtcatggtg ccagataaga
     5101 attggttctc cgactccaat cagggggtca cagcagaacg ggttcgagag ggatgcccgt
     5161 gaaatcttca gcgggggttg ttcctgaaaa gacggtatgg cttgcccggt cacaccctgc
     5221 cgggaacgaa tatcaacctt taactgatcg tattttccac atactcctgt tgcagatccg
     5281 gccccatgga aagcgccgga gcaccaggta cagggattga catgatcgac aacctgcttg
     5341 aaggaaacaa gaagttcagg gaaggattct tcaaagagaa cagggcagtg tatgatgaac
     5401 tctccaaagg ccagagcccc tcggtgctct ggatcgggtg ttctgattca cgcctccaga
     5461 cagggcacat cacccagacc atgccaggca cgctcttcat ccagcggaac atcggcaatg
     5521 ttgccccgct ccatgactgg aacttcgcca ccgtgctcga atacgccatc cgtcatctga
     5581 aggtaaagga tatcgtgatc tgcgggcact cggactgcgg tgcgatca
//
LOCUS       DAOOCO010000133         5591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13657_length_5591_cov_6.343569, whole genome shotgun sequence.
ACCESSION   DAOOCO010000133 DAOOCO010000000
VERSION     DAOOCO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_5591_cov_6.343569"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1811
                     /locus_tag="PK154_07800"
     CDS             276..1811
                     /locus_tag="PK154_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW11000.1"
                     /translation="MSNITVFLDNIAISRDRPALIAPSEGLELTYGEILSRISRTALV
                     LTDQGVGKGDRVCIYLDSCPEHLISYFAIWRIGAVAVPANISLKEDEIGYVIRDSGAR
                     ILIHDNSGASVAMGALTRGNLACRGLNLDQLSASILDTGIYPPVNCSFDDLCQIQYTA
                     GTTGNPKGAMLTQGNWLTSLETEREVLALHENDRYLGIYPMGHVGVSWGLSVLRAGGT
                     YVIMKRFNLPEYLDLCRKYRISVLSGMPPVIHSLCSAPEGTEETLKTVRVIISGGGQL
                     LPTIWEAFDQRYKIPVANAYGLSETVVVGTGTITLPGRPELTGNYLSVGVPTGYSEVK
                     IVDPADHHLSLACGENGEIALRGPSVAKGYWNLPGPTTESFLPEGWFLTGDIGHVDTK
                     GLLYITDRKKDMIIMSGWKIYPTEVENVLLKHPAVLDAAVFGCPDERRGEIPVAVIVQ
                     KEKGSPDPDELLSFCRERLAGYKVPRGIIVTESLPRVHGWKLLRRRLREEYSEFLSSE
                     DLS"
     gene            1849..2433
                     /locus_tag="PK154_07805"
     CDS             1849..2433
                     /locus_tag="PK154_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HPW11001.1"
                     /translation="MQKKDKQTTGVIGLDAVIDGGYPKGSVVIVTGSPLSGIEHVARQ
                     FWRVEVEGGVYLMIDGDVEEEMVDSRNLNPDAFPGYYKGKRIVVDSLSSVILNSGIDD
                     AIQCLLLARKEASRENSTIMFLLYNNIHKPEDENRIFRIADVVLELKVVTFATEVERQ
                     LAVHKLRQGNVPKRLIPFNITDKGIELSTTSRVV"
     gene            complement(2441..3343)
                     /locus_tag="PK154_07810"
     CDS             complement(2441..3343)
                     /locus_tag="PK154_07810"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPW11002.1"
                     /translation="MSSLFPELPLPTTVVGSYPASPRRGLSGIFDPFREAVRTAVNDQ
                     VAAGIDIISDGQVRGDMVRMFTSRLPGVRGNDVIGKVSAPESPITVTDTKYAISRSRR
                     VKGIITGPTTLSFALHVSSPVYRDRADLALDLAHALAPEAAALAGAGAAIIQIDEPIL
                     STGIADLDSAREALEIIVRGIEKPTCLHVCGDLSPVISDLLRMPVHILDFECAKSPGN
                     LDLCRDLDFGRKMVGFGCVDSSDPVPETTEMIRSRIEKALTVFSPDQLLLDPDCGLRM
                     LPQKAAFLKLSRLVEAVKMVREQL"
     gene            complement(3340..4632)
                     /gene="aspS"
                     /locus_tag="PK154_07815"
     CDS             complement(3340..4632)
                     /gene="aspS"
                     /locus_tag="PK154_07815"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HPW11003.1"
                     /translation="MRIPIKEVTPETAQAEVIGWVHEVRDLGGLTFFLIRDRTGILQV
                     TIPKKKVGEEVLATVREVSKESLVRVRGSVKAIEKAPGGRELVPDSFELLARSESPLP
                     LDVVEKVPADLDTRLDARFLDARRPRVTAIFQIRSAVIRTVYDYLFARDFVNITTPKV
                     VAAATEGGTELFPIAYFEKEAFLNQSPQLYKQMMMAAGFERVFEIGPIFRAEEHNTTK
                     HLNEATSIDIEVSFADHRDVMELLEDLILHIYHTVQDRCAGPLEVLDIGELTIPDTPF
                     DRLPYPDAIEMAAGATGEAIAYGDDIGTIAEKAIGEVIGSHYFITDWPTSIKPYYAMP
                     YDDDPTVCKAFDLMHPRMELSSGAARVHQHDLLVRQIRAKGLSPENFEFYLAPFRYGM
                     PPHAGWGLGADRLVMTMLGLGNIREAVLFPRDRHRLTP"
     gene            complement(4705..5349)
                     /locus_tag="PK154_07820"
     CDS             complement(4705..5349)
                     /locus_tag="PK154_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPW11004.1"
                     /translation="MPGPAIELREEVIVALNARYPAIKRLFGVKKIGIFGSLARGDDL
                     PDSDIDIEVEYEPGADTYRNYIGLALYLEDLFGGRKVDLVTSRVLEGYLHSDTTGEHA
                     EKNRDWVYASHMLEEMVFLLERTKSLGFREFARDDLLKRATIRSLEVIGNGASRMSDT
                     TKKNWPGIPWKELAGMKDRLAGMYFSPDWVLVWDIISQEIPSIEPRLRSLAGGL"
BASE COUNT         1291 a         1444 c         1485 g         1371 t
ORIGIN      
        1 gggaggcctt ccaagagacg tgcaaccacc tgaatatcaa taataccttt tctcttcaag
       61 actcttatga acgagtccat tctggttttt tcagacgcag gatgaaagac tactccaccc
      121 tcctccgtac ctaatgaggg tgtatggtga ttctctcagg tattcatacg gaatgccaat
      181 ctcatcttac ccttcgaggg agtgaccgct aaataccatg atgaacaata gtgtacatta
      241 ttgtataata ctactcagaa acgtggagtg ctctgatgtc aaatatcacg gtatttctcg
      301 ataatattgc aataagccgg gatcggcctg ccctgatcgc tccctctgag ggactggaac
      361 tgacgtacgg agagatcctc tcccgaataa gcaggactgc cctggttctc acggaccagg
      421 gagtaggaaa gggagaccgg gtctgtatct atcttgacag ctgccccgag caccttatca
      481 gttactttgc gatatggcgg atcggggcag ttgctgtacc cgccaatatc tccctgaagg
      541 aagacgagat tgggtatgtg atacgtgatt ccggtgccag aatcctcatt catgataatt
      601 caggcgcttc agttgcgatg ggggctctca caagaggtaa tctcgcttgc aggggattga
      661 accttgatca gctgagtgcc tcgatccttg ataccgggat atatcccccg gtcaactgca
      721 gtttcgatga tctctgccag atccagtata ctgcagggac aaccggcaat cccaagggtg
      781 caatgctcac tcaggggaac tggttgacca gccttgagac tgagcgggaa gtccttgcac
      841 tgcatgaaaa tgaccggtac cttgggatct accccatggg gcatgtcggg gtcagctggg
      901 gactttccgt tctccgggcg ggtggcacct atgtgatcat gaaacggttc aatctccccg
      961 aataccttga tttatgcagg aagtaccgga tctcagtact ctcgggaatg ccaccggtca
     1021 ttcattcgct ttgcagtgca cctgaaggaa ccgaagaaac actcaagacc gtgagggtga
     1081 tcatcagtgg cggcgggcag ctcttgccta ccatctggga ggcgtttgac cagcggtaca
     1141 agatacccgt ggcgaatgcc tacggccttt ccgagacggt tgtcgttggc acgggaacca
     1201 ttaccctccc agggcggccg gagctgaccg gcaattacct gagtgtggga gttcccacgg
     1261 gatattccga agtgaagatt gtcgatccgg cagatcacca tctgtcgctg gcatgcggag
     1321 agaacgggga gattgcgctc aggggaccct cagttgcaaa gggatactgg aacctgcctg
     1381 gtcccactac tgaaagtttc ctccctgaag gctggttttt aaccggggat atcgggcatg
     1441 ttgacacgaa gggcctttta tacatcaccg acaggaaaaa ggacatgatc attatgtccg
     1501 gatggaaaat ttacccgacc gaagtggaaa atgttctcct gaaacaccct gcagttcttg
     1561 atgccgcagt tttcgggtgt ccggatgagc gaaggggcga gatccctgtc gcggttatcg
     1621 ttcaaaaaga aaagggcagc cctgatcctg acgaacttct ctccttttgc agggaacgtc
     1681 tggcgggata caaggttccg cgcgggatca ttgttaccga atctctcccc cgcgttcatg
     1741 gctggaaact cttgagacgc aggcttcgtg aggaatattc ggaattcctc tccagtgaag
     1801 acttatctta agcaatggaa tactgtagtg aggagggggc ctcttctctt gcagaaaaaa
     1861 gacaagcaga ccaccggggt catcgggctt gatgcggtta ttgatggggg ttatcccaag
     1921 ggatcggtgg tcatcgtcac cggatcaccc ctctcaggta tcgaacatgt tgcccgccag
     1981 ttctggaggg tggaagtgga agggggggtc tatctgatga tcgatggtga cgttgaagag
     2041 gagatggtgg attcccgaaa cctcaacccg gatgctttcc ctggatacta taaaggaaag
     2101 cggattgttg tcgattcact ctcatccgtc attctgaatt ctggtatcga tgatgctatc
     2161 cagtgtctgc ttctggcaag aaaggaagct tcccgcgaga atagcacgat tatgttcctc
     2221 ctttacaaca atattcacaa gccggaagat gaaaacagaa tctttcgtat tgccgatgtc
     2281 gtacttgaac tgaaagtggt gacctttgca accgaggtgg aacgacagct cgccgtccat
     2341 aaacttcggc agggcaatgt acctaaacgg cttattccct tcaatatcac cgacaagggg
     2401 atagaactct ccacaacatc tcgtgtagtg tgaacactca tcagagttgt tctcgcacca
     2461 tttttaccgc ttcgaccaac cgtgataact ttaaaaaagc ggctttttgt gggagcatac
     2521 ggaggccgca gtccggatca aggagaagtt gatctggtga aaatacggtc aaagcctttt
     2581 cgattctcga tcgtatcatt tctgttgttt ccggaacagg atcggaagaa tcgacgcatc
     2641 cgaatcctac catcttcctg ccgaagtcaa ggtctctgca gagatcgagg ttcccgggcg
     2701 attttgcaca ttcaaaatcg agtatatgga caggcatccg gaggagatcg cttattaccg
     2761 gtgatagatc cccacatacg tggaggcagg tcggtttctc aatcccccgg actatgattt
     2821 ccagggcttc cctggcactg tcaagatcag caatcccggt tgagaggatc ggctcatcga
     2881 tctggatgat tgccgcacct gctcccgcca gggcggcagc ttcaggggca agggcatggg
     2941 ccagatcaag ggcgagatca gcacggtcgc ggtatactgg agaggagaca tgaagggcga
     3001 aggagagcgt cgtcggtcct gttatgatgc ctttcaccct gcggctccgg ctgattgcat
     3061 acttggtgtc ggtaacggtg attggacttt ctggagcaga gacttttcca atgacgtcat
     3121 ttcctctcac ccccggcagc cgtgaagtga acatccggac catgtcaccg cgtacttgcc
     3181 cgtcagagat gatatcgatc cctgcagcca cttgatcgtt cacggctgtc ctgacggcct
     3241 cacggaaagg atcaaagatt ccggatagtc ctctccgtgg gctagccggg taacttccga
     3301 ccaccgttgt aggcaggggg agttcgggaa agagagaact catggtgtca gccggtgtct
     3361 gtcccttggg aagagcacag cctccctgat attacccaga cctagcatcg tcatgacgag
     3421 tcggtcagcc ccgagtcccc agccagcgtg gggcggcatg ccatatcgga atggtgccag
     3481 gtaaaactcg aaattctccg gtgaaagccc ttttgcccgg atctgtctga caagaagatc
     3541 gtgctgatga acccgtgcgg cacccgatga aagctccatc ctcgggtgca tcagatcaaa
     3601 ggccttgcag actgtggggt cgtcatcgta cggcattgca tagtacggtt ttattgaggt
     3661 cggccagtca gtgataaaat aatgagatcc tatcacttcc ccaattgctt tttcggcgat
     3721 ggttccgata tcatcgccat aagcaatcgc ctctccggtt gcaccggccg ccatctcgat
     3781 ggcatcggga taggggagcc ggtcgaaggg ggtatcaggg atagtgagtt ctccaatatc
     3841 taatacttca agaggaccag cacatcggtc ctggaccgta tggtaaatat ggagtataag
     3901 atcttcgagc agctccatga catcccggtg atcagcaaat gaaacctcga tatcgatgga
     3961 ggtggcttcg ttcaggtgtt tcgtggtatt atgttcctca gcccggaaga tagggccaat
     4021 ctcaaagacc ctctcaaatc ctgcagccat catcatctgc ttgtataact gggggctctg
     4081 gttcaggaat gcttcctttt cgaagtaggc aataggaaag agttcggttc ccccttccgt
     4141 agctgctgca actacctttg gggtggtgat gttcacaaaa tcccgggcga agaggtagtc
     4201 atacaccgtg cggatcactg cactcctgat ctggaagatt gcggtcaccc tgggcctgcg
     4261 tgcatcgagg aaccgtgcat caagacgcgt gtcgaggtcc gcaggtactt tctcgactac
     4321 atcgagcgga agcgggctct cgcttcttgc cagcagttca aacgaatcag ggacaagttc
     4381 tctccccccc ggtgcttttt caatcgcctt gacagatccc cggacacgga caagcgattc
     4441 tttcgagacc tctcttacgg tggcaagaac ctcctcccct accttttttt tcgggatggt
     4501 gacctgcaat attcccgtcc ggtcccgtat caggaaaaaa gtgagacccc caaggtcccg
     4561 cacttcgtgt acccacccga tcacctcggc ctgtgcagtc tcgggcgtca cctcctttat
     4621 tggaatccgc atataaaccc gtaagctatg ggagggggga gataatctct tttttcctgc
     4681 ccggtgcagt ttcgcaagct atgttcaaag gcctcctgcc agtgaacgga ggcgcggttc
     4741 aatcgagggg atctcttggg atatgatgtc ccagacaagc acccaatccg gtgagaagta
     4801 cattcctgca agccggtctt tcattccagc cagttctttc catggaatgc ctggccagtt
     4861 cttcttcgtt gtatcagaca tccggcttgc accgttccca ataacttcca gtgaccggat
     4921 agtcgccctt ttcaggagat catcgcgggc gaattcgcga aatccgagac tcttcgtacg
     4981 ctcgagcaaa aaaaccatct cttcgagcat gtgggaagca tagacccagt cgcggttttt
     5041 ttctgcatgt tctccggtag tatcggagtg gaggtaccct tcaagtacgc gggaggttac
     5101 cagatctacc ttcctgccgc caaagagatc ctcaaggtac aacgccagcc cgatatagtt
     5161 ccgataggtg tcagcccccg gttcatactc aacctcgata tcgatatcac tgtccgggag
     5221 gtcatctccc ctcgcgagag agccgaagat ccctattttc ttcaccccaa acagtctctt
     5281 tattgcggga tagcgtgcat tgagggcgac aataacctct tcacgtaatt caatagcagg
     5341 tccgggcata atgaatgact ccttttcagg tcggggcgtt tgaggccacc cctgaatctg
     5401 ggatatattg tccagtgttc tatttatgta gtcttatcag ttgatgcgga cgttggggac
     5461 tcactgttct cccatgaggg tgatgcgccc tgacaaccgt cctctctttg gcgatgtgaa
     5521 ggtggtctgg tcatactcat gggcgagctc tttcggccat caggatgagt gaggattcgt
     5581 gtacttcagt t
//
LOCUS       DAOOCO010000134         5519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13888_length_5519_cov_4.413799, whole genome shotgun sequence.
ACCESSION   DAOOCO010000134 DAOOCO010000000
VERSION     DAOOCO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13888_length_5519_cov_4.413799"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2310)
                     /locus_tag="PK154_07825"
     CDS             complement(58..2310)
                     /locus_tag="PK154_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HPW11005.1"
                     /translation="MRSPFEIIADRVVKKPKMVLLFIAILLAVALVGTSFIVMETGSE
                     TYLDPDTPRSMLLSEYTDTFQSESIMLLVESDDVLNLETLRYLERLEEEISQQRYITG
                     TSSIASMVKQMNGGVLPRSSAELDAVISRIPSEISSRYIPSHLLTIGVITLEPGLSQE
                     TKNSVLDNLDAIVTLSSPPPGVHIVVTGDPAFQQQMGEEIGTSMGTLILIAMVLMVVA
                     VGLFFSHIRYRFLPVGIVASGLILTFGVIGYSPYSITMVAIAAFPVLIGVGIDYAINI
                     QTRFDEEARKSSLEEAARTTITRSGPSILFAMLSTSMGFLALWISPVPMIAGFGVICV
                     IGVACCYLVALLAIPSLGLVLKYRPKHGINNATGSEGNSHLMERYNAAVGTLARKIAR
                     QAVPILLVCALIGVVGFLMDAEIAINTNEETFVPQDMPALINLKKVSRTMGSTASLPL
                     YVRGDNVLDIGTIRWMYEFQQYEEGHNSKITGSESIASLIVAYNNGILPTSNSEIDEI
                     LSRIPGTQKEQYISGTTDAVILFSTVDMENEVAMAMIDNFAQEIEWNEPPPGVSVRIT
                     GMGEMFTNLIKEINVGKTQMTFLAFVMIFGLLLLIYRNAVKALTPVIPIMMIVGWNGL
                     VMYVLGIDYTPMTATLGSLTIGVASEYTILIMERYYEERANGLPVIDSVQQATGQIGT
                     AITVSGMTTVFGFAALIASSFNMISNFGLVTVITVGFSLIGAIIVMPAIIVLMSRFEK
                     TAEGSPAADP"
     gene            complement(2867..3013)
                     /locus_tag="PK154_07830"
     CDS             complement(2867..3013)
                     /locus_tag="PK154_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11006.1"
                     /translation="MAGNERLFLGNDQAVVEHAPLVLSDEDHIAAEQILVRAIREILL
                     HRTS"
     gene            complement(3015..3599)
                     /locus_tag="PK154_07835"
     CDS             complement(3015..3599)
                     /locus_tag="PK154_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HPW11007.1"
                     /translation="MTENELQSIRLEDRDDLTPQEKEAVAFYLEYSNEVQYYLRHPRE
                     LSGLEESPHFQALVHHLDNTISRSSITPGNLVYRGVRADFSKRLLFLLSIDPAECNDG
                     FHRITPQIIRDLGFTSFTTDAERLVSKKKDDPKILFVHQTTPSGNALYIGGEDAELLY
                     PRAYPWMATGFACSRRGRETLIFINLASYDGGIT"
     gene            complement(3885..4334)
                     /locus_tag="PK154_07840"
     CDS             complement(3885..4334)
                     /locus_tag="PK154_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HPW11008.1"
                     /translation="MMNRLPNPLLLILAAVIMLCSCGCISPEMPLSPEDQAHVIAFAN
                     PITDNLLDGFNQNNYTQYSRDFSSEMKKGLDEKVFEQNRALVVSKIGSSVSRGDPVVT
                     GSGEFLVVNYKADFEQEQGVDVRVVFRKGDDTHQVYGFWFNSPKLRS"
     gene            4742..>5519
                     /locus_tag="PK154_07845"
     CDS             4742..>5519
                     /locus_tag="PK154_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW11009.1"
                     /translation="MSKFILICFLILSLESGQVIAASLPPVAAFTATPSSGVAPLTVH
                     FTDQSLNNPTTWVWYFGEGDAGSIVQNPVYTFNNPGTYDVTLAVLNTAGNNRITKQVV
                     VTASQQAPVAAFTATPSSGVAPLTVHFTDQSLNNPTTWVWYFGEGDAGSIVQNPVYTF
                     NNPGTYDVTLAVVNNAGNNRITKQVVVTQQSPSSEEYCFVTKWGSPGWDDGQFYYPAG
                     IAVDNYGAVYVVNSGFTHESIQKFDNNGNFIMKWGSSGSGN"
BASE COUNT         1407 a         1328 c         1452 g         1332 t
ORIGIN      
        1 tcgcagcggg gagggatcat agagaggtag tgggtgcggc gaatggaaaa tagttgttta
       61 tggatcagca gcaggggagc cctctgcggt tttttcgaac cggctcatca ggacgatgat
      121 ggccggcatg acaatgatgg ccccgatgag tgagaatcca acggtaataa ccgtgacgag
      181 gccgaaattg ctgatcatgt tgaaggaaga ggcgatcagt gccgcaaagc caaagacggt
      241 tgtcatcccg gatacggtga tggcagtccc gatctgcccc gttgcctgct gcaccgaatc
      301 gatgaccggc aggccatttg ccctctcctc gtagtaccgc tccatgatca ggatggtata
      361 ttccgaagca acgccgattg tgagggaccc gagcgtggcg gtcatcggcg tataatcgat
      421 gccgagcacg tacatcacaa gcccgttcca cccgacgatc atcatgatgg ggatgaccgg
      481 tgtgagggct ttgacagcat tccggtaaat aaggagcagg agaccgaaga tcatcacgaa
      541 ggcaaggaag gtcatctgtg tcttcccgac attgatctcc tttatcaggt tggtgaacat
      601 ctctcccatc ccggtgatcc tgaccgacac tcccggtggt ggctcgttcc attcgatctc
      661 ctgggcaaaa ttatcgatca tcgccatcgc gacctcgttc tccatatcca ctgtcgagaa
      721 gaggatcacg gcatcggtcg tgccgctgat atactgttct ttctgggtcc cggggatccg
      781 tgacaggatc tcgtcgatct ccgagtttga ggtcggaagg attccgttat tgtaggccac
      841 aatcaggctt gcaatgctct ctgatccggt aatcttgctg ttatgacctt cctcatactg
      901 ctggaactcg tacatccacc gtattgttcc gatatcgagc acgttgtctc cccttacata
      961 gaggggaagc gaggcggtcg atcccatcgt cctgctgact ttcttgaggt tgatcaacgc
     1021 tggcatgtcc tgcggaacga acgtctcctc gttggtgttg attgcgatct cggcatccat
     1081 caggaatccg acaacgccga taagggcaca gacaagaaga atcggcaccg cctgccttgc
     1141 gatcttccgg gcgagggtgc cgaccgccgc attataccgc tccatcaggt ggctgtttcc
     1201 ttcgcttcca gttgcgttgt tgatgccatg tttggggcgg tacttgagga caagtccgag
     1261 agaagggatg gcaagcagcg cgacgaggta acagcaggcg acgccgatga cacagatgac
     1321 gccgaaaccg gcgatcatgg gtaccgggga gatccagagc gccaggaatc ccatggaggt
     1381 cgagagcatg gcaaagagga tcgagggtcc cgatcgggtg atcgtggtcc ttgctgcctc
     1441 ctccagggag gactttctcg cctcttcatc aaatctcgtc tggatattga ttgcatagtc
     1501 gatcccgacc ccgatcagga caggaaacgc ggcaatcgcg accatggtga tcgaatacgg
     1561 ggagtatccg atcaccccaa aggtgaggat gagcccggat gcaacgatcc ctaccggaag
     1621 aaaccggtac ctgatatggg agaagaacaa tcccacggcg acgaccatca ggaccatggc
     1681 aataaggatc agggtcccca tcgaggtgcc tatctcctct cccatctgct gctggaatgc
     1741 cggatccccc gtcacaacga tatgaacccc gggtggtgga ctggagagcg tgacgatagc
     1801 atccaggttg tcaaggacgg aatttttcgt ctcctgcgaa aggccgggct caagggttat
     1861 caccccgata gtgagcaggt gcgaggggat gtatcgtgaa gagatttcag aagggatgcg
     1921 ggagatgaca gcatccagct cggcagagga tcgggggagg acgcccccgt tcatctgctt
     1981 taccatggag gcaatactgg atgtcccggt gatgtaccgc tgttgtgaga tctcctcttc
     2041 gagcctctcg agataccgga gtgtctcgag gttgagcacg tcatcgctct ccacgagcag
     2101 catgatgctc tctgactgga aggtgtcggt atactcagaa aggagcatcg agcggggggt
     2161 atcggggtca aggtaggtct ccgatcccgt ctccatcacg atgaaactgg tcccgacaag
     2221 ggccacggca agaaggatcg cgatgaaaag caggaccatc ttcggctttt tcacgaccct
     2281 gtctgcgatg atttcaaaag gagatcgcat cggttagaac accgtcagta ctaagaggtc
     2341 ggtggtattg ggtattaaag gaattgttgt gaagaagaca acaaaattcc tcatataccc
     2401 cgatgccccc aaaaataatt gatgaaaaag atacctgcaa gaaagaacct ggcatttgtg
     2461 atattccctg aagaatcctt catgcggtgc aggtgaatca gtatgggaga gatgttccgg
     2521 aaaacatccg gtgaagggta atcgaaaatt gcttgtaaac gtggtcctgt gaaaaggtga
     2581 aacacgccag agttctggat caggtcaggt gaccagtcat atcatgggaa aacgggagac
     2641 ttcaggattc ccctgcagag agttaccagg tcactgctgg aagggatgac tcacgatgtc
     2701 agtgctggaa aggaaagact cgcccagtca ttgcgggaat agggacgact taccctgtca
     2761 ctgagtggaa tggaatgatc catctggtct ttgcagaaaa ggaaagactc tcctcgtcat
     2821 tcctggaaaa gaacgtctca cctgatcctg cataagaggg gagacttcag gatgttctgt
     2881 gcagaagaat ctctctgatc gcccttacga ggatctgctc tgcagcgata tggtcttcat
     2941 cagaaaggac cagcggggca tgctccacca ctgcctgatc gttcccaagg aacagccgct
     3001 catttcccgc catatcaggt gatccctccg tcgtatgatg caagattgat gaatatcagg
     3061 gtctccctcc ctcttctgct gcatgcaaat cccgttgcca tccagggata cgccctcggg
     3121 tacagcagtt cggcatcctc tccgccgata tacaatgcat tcccggaggg tgtggtctgg
     3181 tggacgaaca atatcttcgg atcgtctttc ttttttgaaa ccagtctctc agcatcagtt
     3241 gtaaatgaag taaatcccag gtcccggatg atctgtggag tgatgcggtg gaatccgtca
     3301 ttgcattctg caggatctat cgacaggagg aagaggagcc gcttggaaaa atcagccctc
     3361 actccccggt ataccagatt gccgggagtg atggatgatc ggctgatggt attgtcaaga
     3421 tgatgaacca gcgcctggaa atgaggggac tcctcaagtc cgctcaattc cctgggatgc
     3481 cggagatagt actgcacctc gttgctgtat tcgaggtaga aggcaactgc ttccttttcc
     3541 tgcggggtga ggtcatcccg atcctccaag cggatgctct gaagttcgtt ttctgtcaca
     3601 ggagaagata gtacgaaagg agatgagaaa agcgtttcat ttcaatccgg gaatgaggtg
     3661 atggaaggga tctggtacaa caaggtagct gtggcacagg tatctggtcc aacaaggtat
     3721 accagacccg tcatcatcct tccagttctt tggggaaaaa atgacgaggg atgtgaagca
     3781 ggaaggggag aacgagcaag tcgcagaaag gagatcgttc cactcctctt cgttcaaaca
     3841 ggggatctga acataaaaga gaggattact tcaggatccg gggatcagct gcgcaacttc
     3901 ggtgaattaa accagaatcc gtatacctga tgggtatcat ctcctttccg gaatacgacc
     3961 ctgacgtcaa cgccctgttc ctgttcgaaa tctgccttgt aattcacgac caggaactct
     4021 ccgcttcctg tcacaacggg atcacccctg gatacagagg agccgatctt cgagacgacc
     4081 aatgcccgat tctgttcgaa caccttctca tccagaccct ttttcatctc actgctgaaa
     4141 tcccgtgaat actgggtata attgttctgg ttaaatccat ccaacaggtt gtcggtgatc
     4201 ggattggcga atgcaatgac atgtgcctga tcttccgggg ataatggcat ctccggactg
     4261 atacaaccac aggagcagag catgatgact gctgccagga tcagaagcag gggatttggt
     4321 aaccggttca tcatgatgca atgataccct tctgataata atattgatta aaacaatgcg
     4381 agggatggga tgtagaacgg agcgtttaat gcgccccgat tggtggccgc gcatattatt
     4441 gatactttga cgagtgctat ttgccacctc tcccaatgtc aagccttaat agaaaggcct
     4501 tcactcctgt ttatttcttc ccacctacta ctacccacgc cgtttaagtc ctcatgaaag
     4561 cggaaccacc aatatattaa tagaaaatag aaaacgccaa ataatccgaa ttcaataact
     4621 ttagtttaag tgaattaaac ctgcctcaca ctcaaattca tgtttgcaag taccatttat
     4681 gtattttagg gagtctgatg ggctgatatt cacaggattt ggggtaaaaa tgagatttag
     4741 catgagcaaa ttcatcttaa tatgtttttt aattctaagt ttggaaagtg gtcaagtcat
     4801 agcggcatct ctgccacccg ttgccgcatt tactgcaact ccgagttcag gagtagcgcc
     4861 attaacggtt cattttactg accagtcttt aaacaatcct actacttggg tatggtattt
     4921 cggtgagggc gatgcaggca gtattgtaca aaatcctgtt tatacattca ataaccctgg
     4981 gacctacgat gtcacgctcg cagtgctaaa tactgccgga aataatagaa ttacaaaaca
     5041 ggttgttgtt actgcatcac agcaggcacc cgttgccgca tttactgcaa ctccgagttc
     5101 aggagtagcg ccattaacgg ttcattttac tgaccagtct ttaaacaatc ctactacttg
     5161 ggtatggtat ttcggtgagg gcgatgcagg cagtattgta caaaatcctg tttatacatt
     5221 caataaccct gggacctacg atgtcacgct cgcagtcgta aataatgccg gaaataatag
     5281 aattacaaaa caggttgttg ttacacaaca atcaccaagt tctgaagaat attgctttgt
     5341 aacaaaatgg gggtcacctg gatgggatga cgggcaattt tattatccag ctggcatcgc
     5401 ggtagataat tatggcgccg tttacgttgt taattcagga tttactcatg aaagcatcca
     5461 gaaatttgat aataacggta atttcatcat gaagtggggc tcttccggtt ctggaaacg
//
LOCUS       DAOOCO010000135         5506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_13926_length_5506_cov_5.538800, whole genome shotgun sequence.
ACCESSION   DAOOCO010000135 DAOOCO010000000
VERSION     DAOOCO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13926_length_5506_cov_5.538800"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK154_07850"
     CDS             <1..333
                     /locus_tag="PK154_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPW11010.1"
                     /translation="VVFVADPLQVTELVTLVGAILEGTDPVWIPPMAACQQIGAFVYD
                     EARKEHPRAVLGYTDLSARENVGKMIPENMFTFAVPFSPFEEMEAEAEDGVFDGPIGK
                     GLTTDTVR"
     gene            341..1111
                     /locus_tag="PK154_07855"
     CDS             341..1111
                     /locus_tag="PK154_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPW11011.1"
                     /translation="MEKRDFKEIGARLKSAFRLSTRPLAVYGSEVLPSGIPPMSGVNR
                     CFAVSLLRMATGTDVSAIYVSEDTSEGCCHGGLYRVGYAPVSNEIKYFVSTGKKDVRG
                     GAAEYLKASPELVERCDLAAGPIRPPGKYLIVQACETLPDPDIAVRSICCFGTGEQIR
                     NMAALIHFDRDDPFSPVIVPWGPSCATFITFPAGLAENTPRDTAFMGPQDPTQNRSFP
                     PEMVALGIPAEMAARMVSNLDASFVVRRPQVAFPHHGQ"
     gene            1192..2667
                     /locus_tag="PK154_07860"
     CDS             1192..2667
                     /locus_tag="PK154_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW11012.1"
                     /translation="MRNLDPVTKKTADVPAICHTPDVAVTRTGRRIVLLIAVLAGFLT
                     PFDGSAVNIALPAMGMEFHMDAISLSWIATAYLLAAALFLVPFGKIADIYGRKRILLY
                     GIAVFTLSSFVMTMVPTTNLLIAVRVLQGFGGAMIFGTSVAILTSVFPPGERGKALGI
                     YTTAVYLGLTIGPFLGGLLTEYLGWRSIFFINVPIGIVACILILWKFEGEWAECAGER
                     FDLLGSVVYACALIAVMYGLSIVPDATGIVLIAAGIIIGIIFVLYEMRIPMPVLDMRL
                     LTKNRVFAFSNLAALINYSATYAVTFLLSLDLQFTKGFSPEHAGLILIVQPAVMAVLS
                     PIAGRLSDRIDPQIVASAGMACTAFGLFLLAFLTESIPLWYLLACLAVLGAGLSLFSS
                     PNTNAIMSSVEKRYYGVASGMNGTVRLVGQMLSMGVVMMLFSFIIGRAEITPEYYPEF
                     IRSLQYAFVLFTILCLIGIAASLKRGKRRPIPGPGTMAPEK"
     gene            3050..3547
                     /locus_tag="PK154_07865"
     CDS             3050..3547
                     /locus_tag="PK154_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPW11013.1"
                     /translation="MVSPTRDDPVTQWIQEIEVPAALISYTYRTIQKRFAKDLAPYNI
                     GWGHFAILMSLYDEEGRSQDNLALSRGFDKTMIAKSVVKLEEEDLIRRQTDPADKRVK
                     RLYLTEKSRKLQPEMKRIGYGINEFLVKDFDEKESKIVLEYLRKVAMNASELESPLSI
                     SPQDR"
     gene            complement(3997..4857)
                     /locus_tag="PK154_07870"
     CDS             complement(3997..4857)
                     /locus_tag="PK154_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-CTERM sorting domain-containing protein"
                     /protein_id="HPW11014.1"
                     /translation="MPKSIVLLALGLTVAVLACSIPADAVVTPLPGQGNQTGILFGVH
                     PNAKTNETITAFIAEHGEIWWHEGIYNHTHKYVYVVPANACEEQLVYLNKIANEHGGE
                     QSIEQIWIIGCDERLPLAAELRFPEIFAKYPALSNDGGLRSYVENQSPNRYEEEITTR
                     PSRTQVYFINDTGVFTEYIVDLSGREIASTTYVSEENLTVGRAEAEAQALSQQPPDAR
                     VENIVLKVYSSDRIAWEITVRAGRETDIVQVPSDLAPGQSLRTTTPGFGALFAALSVG
                     LSFALYRRRR"
     gene            complement(4868..>5506)
                     /locus_tag="PK154_07875"
     CDS             complement(4868..>5506)
                     /locus_tag="PK154_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPW11015.1"
                     /translation="IEKYNNDASHLFDPDSGENLETSDLYHFPFSEGYRVLLEAAQAA
                     DGTTEPYQSAYEIMQYVNGAMNYTYEDPYLEYIFSDLYMMDHPSPINGKYLGVCDEYG
                     TLYTTFARALGIPTRFLSFTMELPSGNVTGHAIAESWNGNAWIHSDPTWNVFDNPQVY
                     RAAGNTHIDITVYGDADDSYYTQDPNDPTGDGILRYEDFRTQILLGEVPRYN"
BASE COUNT         1145 a         1512 c         1552 g         1297 t
ORIGIN      
        1 gttgtgttcg tggcggaccc gcttcaggtc accgagctcg tgacgctggt cggggcaatc
       61 ctcgaaggca cggatcctgt gtggattccc cctatggccg cctgccagca gataggggca
      121 ttcgtatacg acgaagcaag gaaggaacac ccccgggcag tgcttgggta taccgatctc
      181 tccgcacggg aaaatgttgg caagatgatt cccgagaaca tgttcacctt cgccgtaccg
      241 ttctccccct ttgaggagat ggaagcggag gcggaagatg gagtattcga cggcccgatc
      301 gggaaaggtc tgaccacaga tacagtgagg tgaaatgaat atggaaaaaa gagatttcaa
      361 ggagattggg gcccggttga agtccgcatt caggctttcg accagacctc ttgcagtgta
      421 cggatctgaa gtcctgccat cgggtatccc tccgatgagc ggggttaacc gatgttttgc
      481 ggtttccctg ttgcggatgg cgacgggcac cgatgtatcg gcaatctatg tcagtgagga
      541 tacgagcgag ggttgctgtc atggggggct ttaccgtgtc ggatatgctc ccgtatccaa
      601 tgagatcaag tattttgtat cgaccgggaa gaaagacgtg cgcggcgggg cggccgaata
      661 cctgaaggca agcccggaac tcgtcgagcg gtgcgacctg gcggcagggc cgatccgtcc
      721 tcccggaaaa tatctcatcg tgcaggcatg cgaaaccctt ccggatccgg atatcgcagt
      781 cagatcgatc tgttgcttcg gcacgggcga gcagatccgg aacatggcgg cgctcatcca
      841 tttcgaccgt gacgatccgt tttcaccggt cattgtccca tggggtccct cctgtgcgac
      901 cttcatcacg ttcccggcag gccttgcgga aaacacgccc cgcgacactg cgttcatggg
      961 cccgcaggac ccgacacaga accgttcctt ccccccggag atggtggcgc tgggtatccc
     1021 cgcggaaatg gcggcacgga tggtaagcaa cctcgacgcc tcgttcgtcg tccgccggcc
     1081 acaggtggcg ttcccccatc acggacaatg atttgtcccg gttattagcc gggacacgac
     1141 gtgattgcag ccgaagtgtc gagtgcaaaa ccattttttc atggagaaat tatgcggaat
     1201 ttggatcccg taaccaaaaa aactgcagac gttcccgcga tctgccatac gccggatgtc
     1261 gccgtgaccc ggaccggcag gcgtattgtg ctcctgatcg ccgtactcgc cggttttctc
     1321 accccgttcg acggttcggc agtcaacatt gcgttgccgg cgatgggcat ggagttccac
     1381 atggacgcga tctcgctctc ctggatcgcg accgcctacc tcctcgccgc tgcccttttc
     1441 ctggtaccgt tcgggaagat tgccgacatc tatgggagaa aacggatcct cctgtacggc
     1501 atagcggtct tcaccctctc gtcctttgtg atgacgatgg tgccgacaac gaacctgctc
     1561 atcgcggtca gggtcctcca ggggttcggc ggcgccatga tcttcggaac aagcgttgcc
     1621 atcctgacat cggtcttccc cccgggcgag cggggaaaag cgctggggat atacaccact
     1681 gccgtgtacc tcggcctcac gatcgggccg ttcttaggag gcctgctgac cgagtatctc
     1741 ggctggcgga gcatcttctt catcaatgtt cccatcggga ttgtcgcctg tatcctcatt
     1801 ctctggaaat tcgagggcga gtgggcggaa tgcgcgggcg agcgtttcga tcttctcggt
     1861 tcggtcgtct atgcctgcgc cctgattgcg gtgatgtacg gcctttccat cgttccggat
     1921 gcgacaggca ttgtgttgat tgcggccggc atcatcatcg ggattatctt tgttctgtac
     1981 gagatgagaa ttcccatgcc ggttcttgac atgcgactcc tgacaaagaa ccgggtcttt
     2041 gcgttttcaa acctcgccgc actcatcaat tacagcgcaa catacgccgt gacctttctc
     2101 ctgagcctcg atctccagtt cacgaaaggg ttctccccgg aacatgccgg gctgatactc
     2161 atcgtccagc ccgcggtaat ggcagtactc tcacccattg ccggccgact gtcggaccgg
     2221 atcgatccgc agatcgtggc gtcggcagga atggcatgta ctgcctttgg tcttttcctg
     2281 cttgccttcc tgacggaatc aatccccctg tggtacctgc ttgcctgcct ggcggtgctg
     2341 ggtgcgggcc tgagcctgtt ctcctctccg aatacgaatg cgatcatgag ttcggtggaa
     2401 aaacgctact acggtgttgc gtccggtatg aacgggaccg tgcgtctcgt tggccagatg
     2461 ctttcgatgg gtgttgtgat gatgttgttc tccttcatta tcggcagggc ggagataaca
     2521 ccggagtatt acccggagtt catccgcagc ctgcagtatg cattcgtgct cttcacgatc
     2581 ctctgcctta tcgggatagc agcgtctctc aagcgaggaa agcgccggcc gattcccggg
     2641 ccggggacga tggcaccgga aaaataacca ctttttcagg aacggccttt cttgaccggg
     2701 gcgtaacaag cgttctcttg caagcattcc gtagataaat ttgccgaaga gcggtgtgaa
     2761 aatagtaagt gatgacaacc ctactatgcc tgcaatagca gacccccgtt gccggttcgt
     2821 tcccgaggac cggttgttcg gaccggtacg gcatcgccgg tgcaggggtc aagaaaccca
     2881 gcaatgacga aatttacgag gagtaaccag ccggttttct gtctccagcg ctcggaattc
     2941 cgcaggtacg cgatcgcgac aagaacccaa ttgccttcgg aatcattcag cagtgtcgat
     3001 tcaatcttgt ataaataaat gacaatgcaa ccaattagta ggtctgaaga tggtgagccc
     3061 gaccagagat gatccggtta cccaatggat tcaagagata gaagtcccgg cggcgttgat
     3121 ttcgtatacc tatcggacaa ttcagaagag atttgcgaaa gatcttgcgc cgtataatat
     3181 tggatggggg cattttgcga tccttatgtc cctttatgac gaagagggac ggagtcagga
     3241 caacctcgca ttgtcacggg gttttgacaa aaccatgatt gcaaaatccg ttgtgaaact
     3301 tgaggaagaa gatctcatca ggcgccagac tgatccggca gataagcggg taaaacgatt
     3361 gtacctgacg gaaaaaagca ggaaactcca gcctgagatg aaacgtatcg gctatggaat
     3421 aaacgaattt cttgtcaaag atttcgatga gaaagaatcg aaaattgtcc tcgaatattt
     3481 acgcaaggtc gccatgaacg catcggagct ggaatcccct ctctccattt ctcctcagga
     3541 tcggtagttc gctgaattaa aaagggtgtt tatgggatcc agatgattga ttcgagcccc
     3601 tatggccact tcagcactat agaatttact ttttatgccc tggatcggcc tttttttgga
     3661 catggcctca tgtggtggac tgtggtggct atcgccattg gggatggggc tgacggggag
     3721 gcgggaaccc cctctcctgt ttccaacgcg cgaagatccg tgcactgcat cgaccctatc
     3781 ccgcccggcc tcaggcttcc tcctctgcac ctccggtgct caaattcctg ctgtccgcta
     3841 cgctcacggc agtcgttccc cgccctaagg gcaggggcag tcatggcgat acctgatgga
     3901 aagccgtacc tcggagtatc cctgattgag gtgatctgga acaaacgggt gggatttcaa
     3961 caaagcccta caaagggtct atccgccgct ccgaccctat ctccgcctcc ggtacagggc
     4021 gaaactcagc ccgacgctaa gagccgcgaa aagcgccccg aaccccggcg tcgtcgtccg
     4081 gagcgactgc ccgggcgcca ggtccgaggg gacctgcacg atgtccgtct cccgaccggc
     4141 ccggacggta atctcccagg caatccggtc gctgctgtag actttcagca cgatgttctc
     4201 gacgcgggcg tccggaggct gttgggagag ggcctgcgcc tccgcttcgg ctctcccgac
     4261 cgtgaggttc tcttctgaga cgtaggtggt ggaggcgatc tctcttcccg aaaggtcgac
     4321 gatgtactcc gtgaacacgc cggtatcgtt gatgaagtag acctgcgtcc gcgaaggtct
     4381 ggttgtaatc tcctcctcgt agcggttcgg ggattgattc tcgacgtacg atcgcaaccc
     4441 tccgtcgttg ctgagcgccg ggtactttgc aaagatctcc gggaaccgga gctccgcggc
     4501 caggggcagc ctctcgtcgc agccgatgat ccagatctgc tcgatggact gttcgccacc
     4561 gtgttcattg gctatcttgt ttaagtagac gagctgttct tcgcaggcgt tcgccggcac
     4621 gacgtacacg tacttgtggg tgtgattgta aatgccctca tgccaccaga tctccccgtg
     4681 ttcggcgatg aacgcggtga tcgtctcgtt ggtcttcgcg ttcgggtgga ccccaaacag
     4741 gatacctgtc tgatttccct gcccggggag gggcgtcact accgcatctg cagggatact
     4801 gcaggcgagc accgcaacgg tgagaccaag tgccaggagg acaatacttt tcggcataaa
     4861 aattgggtta attgtacctc ggaacctctc cgaggagaat ctgtgttctg aaatcttcgt
     4921 acctgagaat gccatcaccg gttgggtcgt ttggatcctg tgtgtaatag gagtcatcgg
     4981 cgtcaccgta gacggtgatg tcgatatggg tgtttcccgc tgccctgtaa acctgaggat
     5041 tgtcaaatac attccatgtc ggatctgagt ggatccaggc attcccgttc catgattctg
     5101 caatagcatg ccctgttaca ttaccggaag gcagctccat ggtgaatgac aggaaccgcg
     5161 tcgggatacc cagcgcccgg gcgaaagtgg tatacagggt tccatactca tcgcagactc
     5221 ctagatactt tccgtttatg ggagagggat gatccatcat gtacaggtcg gagaatatat
     5281 attcaagata tgggtcttcg tatgtgtaat tcatggcccc attgacatat tgcattatct
     5341 cgtaggcaga ctgatacggc tctgtcgtac catcagccgc ctgcgctgct tcgagtagca
     5401 ctctatatcc ctcactgaac gggaaatgat acaggtcgct cgtctcgagg ttttctccgc
     5461 tgtcaggatc gaagagatgc gacgcatcgt tgttatactt ctctat
//
LOCUS       DAOOCO010000136         5429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14152_length_5429_cov_4.788798, whole genome shotgun sequence.
ACCESSION   DAOOCO010000136 DAOOCO010000000
VERSION     DAOOCO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14152_length_5429_cov_4.788798"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK154_07880"
     CDS             complement(<1..434)
                     /locus_tag="PK154_07880"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW11016.1"
                     /translation="MMEIYKGTRLSVERIKVRLPNGQERDRVVVHPGDAVAVLPVRAD
                     MSCYLIRQFRYPIGTSILEAPAGTLNPGEKPEETAYRELIEETGFQAATLIPRGFLYT
                     TPGFSDERIFLFEAHDLSPSCLYEKDEDEQIEVIRLPLSDIPP"
     gene            complement(434..1102)
                     /gene="queC"
                     /locus_tag="PK154_07885"
     CDS             complement(434..1102)
                     /gene="queC"
                     /locus_tag="PK154_07885"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPW11017.1"
                     /translation="MKAVCLLSGGMDSATLVYYAHEQGYQVSCLHFNYGQRTERKERI
                     SAHTIATLIGAEEFIEVDLNYLTRFGGSSLTDRSLTVDTFQEDRTEIPNTYVPFRNAN
                     LLSIATSFAEARGAGAIFIGVQSQDYSGYPDCRPQFIEAFQRLIDVGTRDGTSIRIMA
                     PFITMTKSEILAIGTRMNVPYEHTWSCYQNEDRACGVCGSCHFRRAAFESLGLCDPIT
                     YEED"
     gene            complement(1102..1710)
                     /locus_tag="PK154_07890"
     CDS             complement(1102..1710)
                     /locus_tag="PK154_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW11018.1"
                     /translation="MKIIEIFRSIQGEGSSQGYPTIFLRLAGCNLNCTWCDTRYAREG
                     GTDVSCEEILQEIAEFTGNRLCITGGEPLLQGDQLLPLLAILHKKGYQIVIETNGTRD
                     FRPFQPYASICMDIKCPSSGEKSDHSLLGFISEQDSVKFVVGDSDDCRYAEEVLTSHP
                     IRGEIFFSPVHGADYGAVAEYIMKRNLPGRIQLQLHKIIGVR"
     gene            complement(1697..2110)
                     /locus_tag="PK154_07895"
     CDS             complement(1697..2110)
                     /locus_tag="PK154_07895"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW11019.1"
                     /translation="MNSRIYKSVHFDASHRLMHYHGKCAHLHGHRWHVEVWLEGKVDD
                     TTGILVDYNTIKSIIERFDHQTILNQDDPMVSCISQFQPVVTTKGDPTSELLAGLLRD
                     TIDAECRDSGLVAHVVRIRVWESETCFAELRHEDH"
     gene            2275..2703
                     /locus_tag="PK154_07900"
     CDS             2275..2703
                     /locus_tag="PK154_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPW11020.1"
                     /translation="MGQGKFAARKLTRDAKKFRWSDPKYVRSHSGVNVKSDPLEGAPQ
                     ARGIVLEKIGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRYVVTKVNNVCLHEMVIGRKEKPRR"
     gene            2709..3290
                     /locus_tag="PK154_07905"
     CDS             2709..3290
                     /locus_tag="PK154_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPW11021.1"
                     /translation="MAETEAGQRLLFNRWDLSEVQIADPGLVRYVGLNPVIVPHTTGK
                     FTRQEFNKANMLIVERLINRLMKSEMNTGNKQLATRIVRDAFEIVHQKTKKNPVQVLV
                     EAIANTGPREETVRLKYGGINVPKSVDTAPIRRVNTGLYFISEAVYKAAHKNKKPVAA
                     CLADELIAASKGDVKAFAVNKKEERERIAKSAR"
     gene            3315..>5429
                     /locus_tag="PK154_07910"
     CDS             3315..>5429
                     /locus_tag="PK154_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HPW11022.1"
                     /translation="MARGRKSIDRVKELMNQPEHIRNIGIVAHIDHGKTTLSDNLLAG
                     AGIISEELAGKQLFMDSDEEEQARGITIDASNVSMVHEYGGQEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVLVDAVEGTMPQTETVLRQALKEQVRPVLFINKVDRLINELKVD
                     EMEMQIRLGKVIDKVNKLIKGMNEESYKNGWKLDASLGTVAFGSALYNWAVSVPYMKK
                     SGVSFKEVYNRCRSGDMKYLAKNSPLSEVLLDMVVRHLPNPVDAQKRRIGAIWHGDKE
                     TKEGKSMLSCDPNGPVALMVTDISFDPHAGEVATGRLFSGRLKRGDGLYVMGSAMKEN
                     RLQQVGIFMGPKRVEVDEIVAGNIAAVTGLKDAIVGSTVTSLMEMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTLVITINEETGEHLISGMGELHLEIITGRI
                     KRDKGVEIITSEPIVVYRETVTQKVEAVEGKSPNRHNRFYFDIEALPQEIVDLIKNGE
                     VSMNQQALERRDVLMKAGMDKDEAKSIKDIKGTNMLIDMTKGIQYLNETMELIIEGIH
                     EALAGGPLADEPVQNLKMRLVDVKLHEDAIHRGPAQVIPAVRSAIKAGILLAGDSLLE
                     PVQKIQITVPTDQMGSATSQIQGRRGQVFDMQSEGDTITVVGKAPVAELFGFAGDIRS
                     ATEGRAMWSTEFAGFEIVPAGMV"
BASE COUNT         1387 a         1352 c         1500 g         1190 t
ORIGIN      
        1 ggagggatgt ctgaaagagg gagccgaatc acttcgatct gttcgtcttc gtccttctca
       61 taaagacagg atggtgaaag gtcatgggct tcgaagagga atatacgttc atcgctgaat
      121 ccgggagtgg tgtagagaaa gccccttggg ataagggtgg cagcctggaa accggtctct
      181 tcgatcaatt cccggtacgc ggtctcttcc ggcttttctc cgggattaag cgtccctgcc
      241 ggagcttcaa gaattgaagt gcctattggg taacggaact gccggatcag gtagcagctc
      301 atatccgctc tgacggggag gaccgctacc gcatctcccg ggtgaaccac cacccggtct
      361 ctctcctggc cattcggaag gcggactttt attctctcta ccgataacct ggttccctta
      421 tagatctcca tcatcagtcc tcctcatagg tgatgggatc acaaagaccg agcgattcaa
      481 aagcagccct cctgaaatga caggatccgc agacaccgca tgcccgatcc tcattctggt
      541 aacatgacca ggtatgttca taggggacgt tcatgcgtgt accgatcgca agtatctcgc
      601 tctttgtcat ggtgatgaag ggtgccataa tccggatgga ggtcccgtcc cttgttccga
      661 catcgatcaa ccgttgaaaa gcctcaatga attgcgggcg gcaatcggga tacccgctgt
      721 aatcctggga ctggactcca atgaagatag ccccggcgcc cctggcttca gcaaagctgg
      781 tcgctattga aagaaggttt gcgttacgga atggtacata ggtgttcgga atctccgtgc
      841 ggtcttcctg aaacgtatca acagtgaggc tgcggtcagt gaggctgctt ccgccgaacc
      901 gggtcaggta gttcaggtca acttctataa attcttctgc accgatcagc gtagcgatcg
      961 tatgggccga aatcctctct tttcgctcag ttcgctggcc gtagttgaag tggaggcagg
     1021 agacctgata cccctgctcg tgggcataat aaaccagggt tgctgaatcc ataccccctg
     1081 ataacaaaca gaccgccttc atcatcttac tcctattatt ttatgcaact gcagttgtat
     1141 ccgtcctgga aggttccgct tcataatgta ctctgcgact gcaccataat ccgctccgtg
     1201 aacaggagag aagaatatct cgccccggat cgggtgagag gtgaggacct cttcagcata
     1261 cctgcaatcg tcactatcac cgaccacgaa cttgacactg tcctgttcag agataaaacc
     1321 aaggagggag tggtcactct tctctccaga cgaagggcat tttatgtcca tacagatcga
     1381 ggcatacggc tggaaaggtc ggaaatccct tgttccgttc gtctcgatga caatctggta
     1441 tccttttttg tgaaggatag ccagaagagg caggagttga tccccctgca gaagaggttc
     1501 acccccggtt atgcagaggc ggttgccggt gaattcagcg atctcctgga gaatctcttc
     1561 gcaggataca tcagtaccac cttcacgggc atacctggta tcgcaccagg tacagttcag
     1621 gttacagccg gctaaccgaa gaaatatagt gggatagcct tgcgagctgc cctcaccctg
     1681 gatgctcctg aatatctcaa tgatcttcat gtcttagctc agcaaagcag gtttcggatt
     1741 cccagacccg tatcctcacg acatgtgcaa ctaaccctga gtctctgcat tctgcatcga
     1801 tggtatccct gagtaatcct gcaagaagct cactcgtagg atcgcctttg gttgtgacga
     1861 caggctgaaa ctggctgata caggatacca tcggatcatc ctggttaagg atggtctggt
     1921 ggtcgaaacg ctctattatg gatttgatag tattgtagtc aacaaggatc ccggtggtgt
     1981 catccacttt tccctcaagc catacttcca catgccatcg gtgaccatgg agatgcgcac
     2041 atttcccgtg gtaatgcatg agccggtggc ttgcgtcaaa atgaacagac ttgtaaattc
     2101 gggaattcat cagtattggt acacccaaag caggtataat attattatca atgcaggata
     2161 acctattcag gcacaggttg gcgactccac aacatataaa accgttccgc gcgattttat
     2221 ataacacagt ggggcgtatc cacatcgtcg agaccaaacc aatgaggata atgcatggga
     2281 cagggtaagt tcgcagcaag gaagctgact cgcgacgcta agaagttccg gtggagtgat
     2341 ccaaaatatg tccggagcca ttctggtgtc aatgtaaaat ctgaccccct tgagggtgca
     2401 ccacaggcac gcgggatcgt gctcgagaag atcggtgttg aggcaaaaca gccgaactcg
     2461 gcaatcagaa aatgtgtgcg ggtgcagttg atcaagaacg gccgtcaggt caccgctttt
     2521 gcagtggggg acggggctat caacttcatc gatgagcacg atgaggtcga gatcgaaggc
     2581 ataggcggga gactcggcag gtcaatgggc gatatcccgg gtgtccggta tgttgtgacc
     2641 aaagtgaaca atgtctgcct ccatgaaatg gtgattggaa ggaaggagaa accacgcagg
     2701 tgagcggcat ggcagaaacc gaagcaggac aacgacttct cttcaaccgg tgggatcttt
     2761 ctgaggtcca gatagctgat cccgggctgg tcaggtatgt agggctgaat ccggtgattg
     2821 tccctcacac gaccgggaaa ttcaccaggc aggagttcaa caaggcgaac atgctgattg
     2881 tggagcggct gattaaccgg ctcatgaaat cggagatgaa taccggaaac aaacagcttg
     2941 caacccgtat cgtacgtgac gcgttcgaga tcgttcacca gaagacaaag aaaaaccccg
     3001 tccaggtcct tgtcgaggca attgccaata ccggcccaag ggaagagacg gtccggctga
     3061 aatacggagg gatcaatgtt ccgaaatccg ttgataccgc cccgatcagg cgtgtgaata
     3121 caggtctcta cttcatatca gaggcggtgt acaaggctgc ccacaagaac aagaaacctg
     3181 ttgccgcatg tctcgctgat gaattgattg cagcatcgaa aggcgatgta aaggcttttg
     3241 cagtgaataa aaaagaagag cgcgaacgaa tagcaaagtc cgctcgataa gtaatccttt
     3301 tttgtggtgt tgttatggcc cgaggcagga aatccataga ccgtgtaaaa gagctgatga
     3361 accagccgga gcatatccgg aatatcggaa tcgtagcgca tattgaccat gggaagacca
     3421 ccctttccga caacctgctt gccggtgccg ggatcatcag tgaggagctt gccggaaagc
     3481 aactcttcat ggactcggat gaggaagagc aggcgcgtgg tatcaccata gatgctagta
     3541 acgtatcaat ggtgcacgag tatggcgggc aggaatatct catcaacatg attgacaccc
     3601 ccggacatgt tgacttcggt ggcgatgtta cccgtgcaat gcgtgcggtg gacggggctg
     3661 ttgtcctcgt cgatgcagtc gagggtacca tgccccagac agagacggtg ctcaggcagg
     3721 cgctcaagga acaggtccgc ccggtccttt tcatcaacaa agttgatcgt ctcatcaacg
     3781 agctcaaggt agacgagatg gagatgcaga tccggcttgg aaaagtgatc gacaaggtca
     3841 acaagctgat caagggcatg aacgaagagt catataaaaa cggctggaaa ctggacgctt
     3901 cacttggaac ggtagcattc ggctccgccc tctacaactg ggcggtctcg gttccgtaca
     3961 tgaagaagag cggggtctcc ttcaaggagg tatacaaccg gtgccgttca ggggatatga
     4021 aataccttgc caagaacagc cccctctccg aggttttact cgatatggtg gtccgtcacc
     4081 tgccaaaccc cgtagatgcc cagaaacgac gtatcggagc gatctggcac ggggacaagg
     4141 aaacaaagga aggaaaatcc atgctcagct gcgacccgaa cgggccggtt gccctgatgg
     4201 tgactgacat ctcctttgat ccccacgcag gagaagttgc aacaggacgt ctcttctcag
     4261 ggcgcctcaa gcggggtgat ggtctctatg tgatgggttc ggcaatgaaa gagaaccgtc
     4321 tccagcaggt cggtatcttc atgggaccga aaagagtcga ggtcgatgag attgtggccg
     4381 ggaatattgc agcagtgacc ggtctcaaag atgccattgt cggatccacc gtgacttcgc
     4441 tcatggaaat gacccccttc gaatccctca agcactatag tgaaccggtc atgaccgtgg
     4501 cagtcgaggc caaaaatatg aaggacctgc caaagctcgt tgaagtgctc cgccaggtgg
     4561 ccaaagagga cccgaccctt gttatcacca tcaacgaaga gactggagaa cacctcatct
     4621 cggggatggg ggaacttcac cttgagatca tcaccgggcg tatcaagagg gacaagggtg
     4681 ttgaaatcat cacttccgaa cctattgtcg tctatcgtga aacagtgacc cagaaggtgg
     4741 aagcggtcga aggaaaatcg ccaaaccggc acaacaggtt ctattttgac atcgaagcac
     4801 tgccccagga gattgtggac ctgatcaaga atggcgaggt ttccatgaac cagcaggcct
     4861 tggaacggcg tgatgttctg atgaaggcgg gaatggacaa ggacgaggca aagagtatca
     4921 aggatatcaa agggacgaac atgctgatcg acatgaccaa gggtatccag tacctcaacg
     4981 agacgatgga actgatcatc gaaggtattc atgaggccct tgccgggggc ccccttgccg
     5041 atgagcccgt ccagaacctc aagatgcgtc ttgtcgatgt gaagctccac gaggatgcaa
     5101 tccacagggg tccggcgcag gtcatcccgg cagtgcgaag tgcaataaag gctggaattc
     5161 tcctcgctgg agactcgctc cttgagcctg tccagaaaat ccagatcacc gttcccaccg
     5221 accagatggg aagtgcaacc tcccagatac agggaaggag ggggcaggtc tttgatatgc
     5281 agagtgaggg cgacaccatc accgtggtcg gaaaggcacc cgtcgccgag ctcttcgggt
     5341 ttgccggcga tatcaggtct gctactgagg gccgggcgat gtggagtacc gagtttgctg
     5401 gatttgagat cgtccctgca ggtatggta
//
LOCUS       DAOOCO010000137         5381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14327_length_5381_cov_5.135186, whole genome shotgun sequence.
ACCESSION   DAOOCO010000137 DAOOCO010000000
VERSION     DAOOCO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14327_length_5381_cov_5.135186"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            575..790
                     /locus_tag="PK154_07915"
     CDS             575..790
                     /locus_tag="PK154_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11023.1"
                     /translation="MGTKIGKEKIKREPGYLYYLGKDGYVWAAPMKNNKGGKKKKVGT
                     EKIAREKGFLYYLGKDGFVGKAKMKNA"
     gene            complement(1082..1777)
                     /locus_tag="PK154_07920"
     CDS             complement(1082..1777)
                     /locus_tag="PK154_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPW11024.1"
                     /translation="MHYEIRYGPSYSMLVVTLEPGDSLTAESGAMTYMQPEIDVKTQK
                     REKSFWGSLSLALVGGQSFFVNEYVATGQKGEVAFSAAPLGDIDSLTVSQGQGFLVQK
                     SAYLASQEGVDLDVKWEGFTKGLFGQGLFMIRATGNGLLFINTFGAIDKHVLAPDESL
                     IVDNFHLVAFSDSCHYVVRKFGGLKETILGGEGLVTEITGPGEVYIQTKNIRELSDWI
                     WTLIAPRVQAKAR"
     gene            complement(1935..2006)
                     /locus_tag="PK154_07925"
     tRNA            complement(1935..2006)
                     /locus_tag="PK154_07925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1971..1973),aa:Arg,seq:ccg)
     gene            complement(2206..3234)
                     /gene="hypD"
                     /locus_tag="PK154_07930"
     CDS             complement(2206..3234)
                     /gene="hypD"
                     /locus_tag="PK154_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPW11025.1"
                     /translation="MAIGKEISEKINDLVDRDYAFMHICGTHEAAIARHGLRSLLPAR
                     LKIVMGPGCPVCITPQGEIDAALDLVERGVILATYGDLLRVPGSAGSLESSGGGVRVV
                     QGVHKAVEIAKGTDKEVVFISVGFETTAPTVAATILSKPPENFSILSCHRLVPPAMKW
                     LLEQGEAHLQGFMLPGHVCTVTGYQDYEQFPVPQVVAGFEAEDILLGLLMLVQQVRAG
                     AHRIDNAYPRAVCREGNPKAKALMYQVFETADVEWRGFPVIPASGLRLKKEFEHYDAQ
                     KKFEIEFKKVSKHTACICDKVLRGIAQPSECRLFDRVCTPRTPVGPCMVSHEGACKIW
                     HTYKEKHQ"
     gene            3279..4382
                     /locus_tag="PK154_07935"
     CDS             3279..4382
                     /locus_tag="PK154_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW11026.1"
                     /translation="MNNETGPNSTLFVPTEIFGISGNFLAIIIFLAGIVIAFTMYGIV
                     KWLKKKADTTESRIDDIILASIGTPAVIAVLVISVFAALQVASLPPRLEGVVESKYFE
                     AVYVIIGAWIVSGFVHNFISIYGSRIAGKAGSDIDERMVTIALTISKYVIWFVAFLLI
                     LLVLDIDITAFLAGAGIVAIAIGLAAQDFLSNFLGGAVIAVDKPFELYDRIKIDQYTG
                     DVMHVGSRSTRLKTLDNQIVTIPNSTITKSFVVNYSTPDTRMKIRVPIGVAYGTDVQQ
                     VKKILNDIAIELAERIPFILSDPEPVVYFLEFGESSLKFELDLWTSDVTKTWEVRDAV
                     NMRIAERFTQEEIEIPFPRMDVRIRDSNNKSNF"
     gene            4575..4647
                     /locus_tag="PK154_07940"
     tRNA            4575..4647
                     /locus_tag="PK154_07940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4608..4610,aa:Thr,seq:ggt)
     gene            complement(5154..>5381)
                     /locus_tag="PK154_07945"
     CDS             complement(5154..>5381)
                     /locus_tag="PK154_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11027.1"
                     /translation="DAQGYGNLAVWDDGTTRIIEPGNIPLINGLPPRAVFKPLPLVGG
                     FPMLDHALYNSSLQEKIEGVIRNSGGEISRD"
BASE COUNT         1422 a         1280 c         1238 g         1441 t
ORIGIN      
        1 tgcagatcgg tgtcttgaat aaccgggaat ataccttacc cgttattcag tgttccacgg
       61 aaaaagtgag agcgcatcaa cactacttta ttttgacagt gactcttaaa attcatctct
      121 tcttgactgc atctgatatg gaatgacaga atattcccct gtttcaatga acaatacggt
      181 actatcttga tttccttaaa tattcatcaa atccgtcttt caatcaaatt ttaaaaagaa
      241 ttctactcgc atgatattgt gttttcggtg tgttgtctct tatttcctga tacaaaagga
      301 ccccctggaa aatgagagtc gaatacggaa ctgttgtgcg gcatcaccac gatacattcc
      361 ggaaaatttt ttttggctgg cgataatttg agagaagtaa taattattgg atattatttc
      421 atactaattg ataaagaaat aggtgttaat ggatacttca cggcgaaaga caggtatgca
      481 cggcggcctt cgggggaatc tcagcgaaac atttaaatac caacttgcgt tatcactttt
      541 gtataaacca aaacttaaaa atcaaggtga atttatggga acaaagattg gaaaagagaa
      601 aatcaagcgt gaacccgggt acctctatta tctgggtaag gatgggtacg tatgggcagc
      661 accaatgaag aacaacaaag gcggaaagaa gaagaaggtc gggactgaaa agatcgcccg
      721 agaaaaaggc ttcctttatt atcttggcaa ggacgggttt gtcggcaaag ccaagatgaa
      781 gaacgcctga actcttcttt ttttcttgtt atcctgaagc agagaatttc tctcttcgga
      841 attgtctttt ccaccggcgg gtatttttcc cagacgcaga ttcctgggct tgagagaaat
      901 aatacgccgg atcaacttcc tgctgaatca gccatatcat attgcaaaga ggaacaaaac
      961 gaataaatgt cggggcgaaa tcgcacccat tttatgggtg gcccaaatcc ccgaagtcct
     1021 ggtatccggc gtatatttgt aatcaactag cgcaacctgt ttttctcccc tgtggatctt
     1081 tttaccgggc cttggcctgg acccgtggag caataagtgt ccatatccag tcagacaatt
     1141 ccctgatatt ctttgtctgg atgtatactt ctcccgggcc tgtgatctca gtcaccaaac
     1201 cctccccacc aaggatggtc tccttcaatc cgccaaattt ccggacaaca tagtggcaac
     1261 tatcggaaaa ggccacgaga tgaaaattgt caacaatgag ggattcatct ggggccagca
     1321 catgtttgtc gatagcaccg aaggtattga tgaagagcaa gccattccct gttgctctga
     1381 tcatgaagag tccctgtcca aacagacctt ttgtaaatcc ttcccatttc acatcgagat
     1441 cgacaccctc ctgggacgca aggtaggcag acttctgaac gagaaaaccc tggccttgag
     1501 agacggtgag agaatcgata tcaccgagag gagctgctga aaacgccacc tcccctttct
     1561 gtccggttgc cacatattcg ttgacaaaga aactctggcc gccaaccagt gcaagggaga
     1621 gcgatcccca gaagctcttc tcacgtttct gggtctttac gtcaatctcg ggctgcatat
     1681 atgtcattgc acccgattcc gctgtaaggg agtcaccagg ttcaagggtc accacaagca
     1741 ttgagtagga cggtccatac cggatttcgt aatgcatgct taactttgtg aatcctcaat
     1801 acaataaaag ggatgggcaa tcccggatat tgagcgattc acccgatggc catcacagga
     1861 aaagacgcag aaatgtgttc atccgttcaa tgagatgcca gcaggggcac ctcatactac
     1921 ccggaaaaag ataacgcccc ggccgggatt cgaacccggg tcggaagctc cggaggctcc
     1981 caggatatcc actaccctac cagggctgac aaaaggcaca tggatgtgtc tccatatgaa
     2041 caggatgttg aagagtataa taattatggg attgctcgca tggtcctctg tattctattg
     2101 cttcaaagaa atgaaactga cgaggtgttc ccccctttag atggggggaa agaacatggt
     2161 ggatttcttt tcaatcgtag ttccggattt tgttagaaaa gaagattatt ggtgcttttc
     2221 tttataggta tgccatatct tgcaggcacc ctcatggctt accatgcagg gtccaacagg
     2281 agttcttggt gtgcatactc tgtcaaaaag tctgcattct gaaggttgag ctattcccct
     2341 gagcaccttg tcacagatac aggcggtatg cttgctgacc ttcttgaact cgatctcgaa
     2401 cttcttctgg gcatcatagt gctcaaattc tttcttgagc ctgagaccag aggcggggat
     2461 aaccggaaaa ccccgccatt ccacatcggc agtctcgaaa acctggtaca tcagagcttt
     2521 tgcttttgga ttaccttctc tgcagactgc cctgggatag gcattgtcga ttcggtgagc
     2581 acccgctctg acctgctgga caagcatcag gagtccaaga aggatatctt cggcttcaaa
     2641 gccggcaact acctgaggta cggggaactg ttcataatcc tggtatccgg tcactgtaca
     2701 gacatgacct ggaagcatga acccttggag atgagcttct ccctgttcga gaagccactt
     2761 catagctggc ggaacaagcc ggtggcagga aaggatactg aagttttccg gcggctttga
     2821 aagaatggtt gcggcaactg taggagccgt agtttcaaac ccgactgata taaataccac
     2881 ttccttgtcc gtcccttttg caatctctac tgctttatgg accccctgga caacccttac
     2941 gcccccaccg gatgattcaa gtgaaccagc actaccggga accctgagga gatctccgta
     3001 ggttgccaga ataacccccc gttcgaccag gtcaagggct gcatctatct ctccctgcgg
     3061 agtaatgcag acggggcacc cgggccccat gacgatcttc agtcgtgcgg gcaacaggct
     3121 ccgcaatcca tggcgggcga tagcagcttc atgcgtcccg cagatgtgca tgaacgcgta
     3181 atcacggtcg acaaggtcat tgatcttttc agaaatttct tttccgatag ccattcctga
     3241 ctaaatattt acgatttgca aagataagta tactcataat gaacaatgaa acaggtccga
     3301 attctaccct gtttgtcccg acagagatat tcggaatctc agggaatttc ctggcgataa
     3361 tcattttttt ggccgggatt gtcattgcat ttacgatgta tggtatcgta aaatggttaa
     3421 agaaaaaagc tgataccacg gaatccagaa ttgatgatat tatcctcgct tccattggta
     3481 caccggctgt tattgcagtt ctggtgattt cagtttttgc cgcactccag gtggcaagcc
     3541 tcccaccccg gcttgaaggg gtggtggaga gcaagtattt cgaagcggta tatgtaataa
     3601 tcggtgcgtg gatcgtttcc gggtttgttc acaatttcat cagcatctat ggttcccgca
     3661 tcgcaggcaa agccgggagc gatatcgatg agaggatggt taccatagct ctcacgatat
     3721 caaaatacgt gatatggttt gtcgcttttc tcctcatcct gctcgtcctt gacattgata
     3781 tcaccgcttt cctggcaggt gcagggattg ttgcaatagc cattggcctg gctgcacagg
     3841 attttttatc aaatttttta gggggggcag ttattgctgt agacaagccc ttcgaactct
     3901 atgaccgaat aaaaattgac cagtataccg gggatgttat gcatgttggt tcccgcagta
     3961 cgaggttaaa aactctggat aatcagattg taacgattcc gaattcaacg attaccaaaa
     4021 gttttgtggt gaattattcc acaccggata caagaatgaa aatcagggta cctatcggag
     4081 ttgcctacgg tactgatgtc cagcaggtaa aaaaaatact gaatgacatc gcaatcgaac
     4141 tggcagaacg tatccctttc atcctctcag atcctgaacc tgtggtgtac ttcctggaat
     4201 ttggcgagtc aagccttaaa tttgagctcg atctttggac gtcggatgtt acaaagacct
     4261 gggaggtcag ggatgcggtg aatatgagga ttgcggaacg atttacccag gaagagatag
     4321 aaatcccctt tccaaggatg gatgtccgca tcagagattc caacaacaaa tccaatttct
     4381 aaaattcact cccctttctt tggactcgac ttccacctca tggagaatat cttgattgta
     4441 ttaacagcga agatccgcgt tcctgccctt tccgggaaga tatttcaggc caggatctca
     4501 acaaattccc tcttttatta tatcttatcc gcaatctttt aaagggttca cctcctaggt
     4561 tattggtgca ccgtgcctca gtggctcagt cggtagagcg tgtccttggt aaggacaagg
     4621 tcgagggttc gattccctcc tgaggctctc ttttccaatt cgttcatttc ccggcaattt
     4681 actccagtta ccacggattc gtcctgtttt cacaagaacg tccccctatc actacagatg
     4741 aggtttgatc gagtcatttg accggaaaaa aagtctcatt ccaaaaaaaa ggcgaagtac
     4801 gtcaagtagc ggaaaggatc tcaatttcag gcatcatacc ggatcctggg aaagaggtgt
     4861 acaggagctg ttctcctctt tcaccattgc aggagaacga ttccactccc tttcatagaa
     4921 ggtctctctc gggcgttgcc ctctgtagag tgttgccgta taagcccccc tgcctgttgc
     4981 acccgtccat cttcccatcc agaatggcat gatttcaatc ctctttaaaa ggaatacctg
     5041 gtcccccaaa accgtattct cttacccgat agttgcgcac ggatcatcaa aatagatttt
     5101 tatgagtaag cttcatcggt ccgatgaata taagataaag ggcagaacgg gggttaatcc
     5161 cgtgatatct ctcctccgga atttcggata actccctcaa tcttttcctg aagtgagctg
     5221 ttgtacaggg catgatcgag catgggaaac ccccctacaa gtggcagcgg cttgaatact
     5281 gctcggggcg ggagaccatt tattaggggg atattccccg gctcgataat ccgtgtcgtt
     5341 ccatcgtccc ataccgcaag gttcccatat ccctgggcgt c
//
LOCUS       DAOOCO010000138         5318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14527_length_5318_cov_6.052062, whole genome shotgun sequence.
ACCESSION   DAOOCO010000138 DAOOCO010000000
VERSION     DAOOCO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14527_length_5318_cov_6.052062"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PK154_07950"
     CDS             complement(<1..894)
                     /locus_tag="PK154_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase subunit alpha"
                     /protein_id="HPW11028.1"
                     /translation="MAKKIERTQKLFLKALKEKFAEDPQSTSTVFAREGLKQSPRKME
                     FVKAGNAAAMARGISMYDPVRCHIGGIPLGQRQLMTYEVSGTGVFVEGDDLHFVNNAA
                     MQQMWDDIRRTIIVNMDLAHQTLQKRLGKEVTPETINEFLHVLNHAMPGAAVVQEHMV
                     ETHPSLVDDCYVKVYTGDDEMADDLEPQFVINIDKLFPAKQAAQLKTAIGKSLWQAIR
                     IPTIVSRTCDGGTTSRWSAMQLGMSFIGAYHMCAGEAATADLAFAAKHAGVIQMAEIL
                     PARRARGPNEPGGIKFGHFADM"
     gene            complement(900..1664)
                     /gene="mcrG"
                     /locus_tag="PK154_07955"
     CDS             complement(900..1664)
                     /gene="mcrG"
                     /locus_tag="PK154_07955"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HPW11029.1"
                     /translation="MAYKPQYGPGSSIVAENRRKQMDPSKKLEKLRDISDEDVVLLMG
                     HRAPGAAYPTAHPPLAEQQEPDCPVRKMVVPLDGAKAGDRIRFIQFADSYYNAPCQPY
                     QRSYVEAYRYRGCDPGTLSGRQIIECRERDLEKIAKELIGTELFDPARTGIRGSTVHG
                     HSLRLAEDGMMFDMMQRCILDKKANMVKYVKNQNSEPLDKEVKVGKPLDEKWLKAHTT
                     IFHSLVGTSFRSDAEYVEYVQRVHSLRTKYGYVPEE"
     gene            complement(1672..2298)
                     /gene="mcrC"
                     /locus_tag="PK154_07960"
     CDS             complement(1672..2298)
                     /gene="mcrC"
                     /locus_tag="PK154_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HPW11030.1"
                     /translation="MPVGRVTQVVDCRAAMGMGKGGGIAQRGTISECRYPDVIVVGMS
                     PGRRHVTKPVCDITSALRREGIEYSISTLVLNAGSGVPPDARDIGGGVLGAYFGLTDT
                     EIQQIERHKVAILHHGNVRSHVVHKVRFILQACDVKAVVVSQAPVDFEDFAKVGVKTA
                     LVMPPPEKVKTRGTVMAIVSGVTRGQTPSREKMAEVIHAVMRLIKTKE"
     gene            complement(2301..2771)
                     /gene="mcrD"
                     /locus_tag="PK154_07965"
     CDS             complement(2301..2771)
                     /gene="mcrD"
                     /locus_tag="PK154_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HPW11031.1"
                     /translation="MTDAIYPQVRIVTVRLLNPETVERVMNLILATEGVRRVILNGPR
                     LPPTITTGPAKGQPNIHEMRKTVKMGEQEVEMQVHVGTILLELENRDVIPAIKAACAA
                     GFGEMQWYLQEGRYMKTDPSLVDYAKYGPYADKAIIGMADPSSKKGPTIIQGIK"
     gene            complement(2786..4099)
                     /gene="mcrB"
                     /locus_tag="PK154_07970"
     CDS             complement(2786..4099)
                     /gene="mcrB"
                     /locus_tag="PK154_07970"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HPW11032.1"
                     /translation="MAKYKDTCDLYDDQGKLLKSGVALEKISPLNNAGILKIIDLTKR
                     TVAVNLAGLDNSIKTGAMGGKNNQILGRAVKVDCVKDADALAAKIGEYVSVNKGDDTK
                     VTKVGGGKMLLIEVPSARLKAAATYDASTTAVAAATTQALIDQYKVGMFDAPYVKSAV
                     WGSYPVTMDMAGGNIISVLSIPQNNEGLGYALRNIPANHAAMMTHRNAMQTCALSATF
                     EQAGEFEMGAAIGPFERAQLLAYAFQGLNANNMVYDIVKANGKTGTIGTVVQSVVERA
                     LEDKVIKAGKKGKSGFIFYETKDPMMWNAYASAGTMAATMVNCGAGRFAQAVSSTLLY
                     FNDLLEHETGLTGADYGRVMGTAVGFSFFSHSIYGGGGPGVFNGNHVVTRHAAGVAIP
                     CVCVACALDAGTQMFGPEQTSKVYQDTFGQIEEFKKPLQAIAKAV"
     gene            complement(4450..5250)
                     /locus_tag="PK154_07975"
     CDS             complement(4450..5250)
                     /locus_tag="PK154_07975"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HPW11033.1"
                     /translation="METVTVTIKTQPELFLEADNISPDAFAGKKAAEITTLHAYEGRE
                     QYTLGKYFDVSGDAGATAADTRIIVKGDVSRVKYIGMKMSAGEVIIEKDADMYVGAWM
                     QGGKLTAKGNVDAFAGIGMKGGELIIEGNAGNYLGAAYRGDWRGMQGGLIRVKGNAGS
                     DIGYFMNGGTIIVEGNADVHIATHAEGGKVVIKGDAKSKIGGQMVEGEIIVFGGIDVM
                     MPGFKYVKDEEIEVDGTKATFALFHGDLGERHPKRKGQVVYGKLYQKI"
BASE COUNT         1018 a         1522 c         1456 g         1322 t
ORIGIN      
        1 catgtcggcg aagtgtccga acttgatgcc tccgggctcg ttcgggccac gggcacggcg
       61 ggcaggcagg atctctgcca tctggataac accggcgtgc tttgcggcga atgcaaggtc
      121 ggcggttgcg gcttctccgg cgcacatgtg gtaggcgccg atgaaggaca taccgagctg
      181 catcgcagac cagcgggagg tggttccacc atcacaggtc ctcgagacga tggtcgggat
      241 gcggattgcc tgccagagcg acttgccgat agctgtcttc agctgggcgg cctgcttggc
      301 cgggaagagt ttatcgatgt tgatgacgaa ctggggctcc aggtcgtcgg ccatctcgtc
      361 gtcaccggta tagaccttca cgtaacagtc gtccacgagt gacgggtggg tctcgaccat
      421 gtgctcctgg accacggctg caccgggcat ggcgtggttg agcacgtgga ggaactcgtt
      481 aatggtctca ggggtaactt ccttgcccag acgcttctgc agggtctggt gggcaaggtc
      541 catgttgacg ataatcgtcc tcctgatatc atcccacatc tgctgcattg cagcgttgtt
      601 gacgaagtgc aggtcgtcgc cctcaacgaa cacgccggtg cccgagacct cgtaggtcat
      661 cagctggcgc tgcccgagcg ggatgccgcc gatgtggcag cggacggggt cgtacatgga
      721 gatgccacgg gccattgctg cagcgttccc ggctttgacg aactccatct tcctcgggga
      781 ctgcttcagg ccctcgcggg caaacacggt gctcgtgctc tgggggtcct cggcgaactt
      841 ctctttcagg gccttcagga agagcttctg ggttctctcg atcttctttg ccatgtcaat
      901 cactcctccg gcacatagcc gtacttggtc ctcagcgagt ggacgcgctg gacgtattcg
      961 acatactcgg cgtcactgcg gaaggaagtt ccgacaagcg agtggaagat ggtggtgtgc
     1021 gccttgagcc acttctcgtc caatggtttg ccgaccttga cctccttgtc cagcggctcg
     1081 ctgttctggt tcttgacgta cttgaccatg ttggccttct tgtcaaggat gcaccgctgc
     1141 atcatgtcga acatcatccc atcctctgcg agacggaggg agtgtccgtg cacagtcgaa
     1201 ccgcggatac cggtgcgggc agggtcaaag agctcggtgc cgataagttc ctttgcgatc
     1261 ttctcgagat cacgctcacg gcactcgatg atctgacggc cggaaagggt tcccgggtca
     1321 cagccgcggt accggtatgc ttcgacatag gaccgctggt agggctggca cggtgcattg
     1381 tagtacgaat cggcaaactg gatgaacctg atacggtctc ctgcctttgc accatcgagg
     1441 ggcacgacca tcttcctgac aggacagtcc ggctcctgct gctcggcgag tggcgggtgg
     1501 gctgtcgggt atgccgcacc aggtgcacgg tggcccatca gcagaacgac gtcctcgtcc
     1561 gagatgtcac ggagcttctc aagtttcttg cttgggtcca tctgcttgcg cctgttctcg
     1621 gcgacaatgg agcttccggg cccatactgt ggtttgtaag ccatttttta atcactcctt
     1681 agttttgatc aacctcatta ccgcatgaat gacttccgcc atcttctctc tcgaaggggt
     1741 ctggccccgg gtgacaccgc tcacgatcgc catgacggtg ccccgggtct tcaccttttc
     1801 gggagggggc atgacaaggg cggttttcac gcccactttt gcaaagtcct caaagtctac
     1861 aggtgcctga gagacaacca ccgctttcac gtcacaggcc tggaggatga acctgacctt
     1921 gtgcaccacg tgcgatcgca cgtttccatg atggagaatg gcgactttat gcctctcaat
     1981 ctgctgaatt tcagtatccg tgaggccgaa gtatgcaccg agcacgcctc ccccgatatc
     2041 ccttgcatcg ggaggcaccc cgcttcctgc attcaggaca agggtgctga tgctgtactc
     2101 gataccctcg cgtcgtaatg ctgaggtgat gtcacatacg ggcttggtga catggcgcct
     2161 tccgggagac atgccgacta cgatgacatc ggggtagcgg cattccgaga tggtgcccct
     2221 ctgggcgatt cctccgcctt ttcccatgcc cattgccgca cgacagtcca cgacctgggt
     2281 tacccgtcct actggcatac ttactttatt ccctggatga tggtaggtcc ctttttgctg
     2341 ctgggatcgg ccattccaat gatggccttg tcagcatagg ggccgtattt cgcgtagtcc
     2401 accagcgaag gatcggtctt catataccgg ccttcctgga ggtaccattg catctcgccg
     2461 aatccggctg cacaggctgc cttgatggca gggatcacat ctctgttctc cagttcaagg
     2521 agaatggtcc cgacgtgaac ctgcatctcc acctcctgct cccccatctt cacggtcttt
     2581 cgcatctcat ggatgtttgg ttgccccttc gcgggaccag tggtaatggt gggggggagt
     2641 cggggaccgt tgagtatcac cctccggacc ccttcagtgg caaggatcag gttcatcacc
     2701 cgttcgacgg tctccgggtt gagcaaccgc acggttacta tccggacctg aggatatatg
     2761 gcatcagtca ttctatcgga atggatcaga ctgccttcgc gatggcctgc agcggcttct
     2821 tgaattcctc aatctgaccg aatgtgtcct ggtagacctt ggaggtctgc tcggggccga
     2881 acatctgggt tccggcatca agcgcacagg caacacagac gcacgggatt gccacaccgg
     2941 ctgcgtgacg ggtcacgacg tggttgccgt tgaagactcc cggaccgccg cctccataga
     3001 tcgagtggct gaagaacgag aatccgactg cagttcccat gacacggccg taatcggcac
     3061 cagtcagacc ggtctcgtgc tcgagcaggt cgttgaagta cagcagggtt gaggacactg
     3121 cctgggcaaa ccggccggca ccgcagttca ccatggttgc tgccatggtt ccggcagatg
     3181 cgtaggcgtt ccacatcatc gggtccttcg tctcgtagaa gatgaagccg ctcttgccct
     3241 tctttccggc cttgatgacc ttgtcttcga gtgcacgctc gacaacgctc tgaacgacgg
     3301 taccgatggt tccggtcttg ccgtttgcct tgacgatgtc atagaccatg ttgttggcat
     3361 tcaggccctg gaatgcgtag gcgaggagct gggcacgctc gaatggcccg attgctgctc
     3421 ccatctcgaa ctctccggcc tgctcgaacg tggcggagag agcacaggtc tgcatcgcat
     3481 tccggtgagt catcattgcc gcgtggttgg ccgggatgtt cctgagtgca tacccgagac
     3541 cctcgttgtt ctgggggatg gagagcacgg agatgatgtt accgccggcc atgtccatcg
     3601 tgaccgggta ggaaccccag actgcggact tgacatacgg ggcatcgaac atgcctacct
     3661 tgtactggtc gatgagtgcc tgggtggttg cggcagctac tgcggtggtt gaggcatcgt
     3721 aggtagcggc tgctttcaga cgggcgctcg ggacttcgat aaggagcatc tttccgccgc
     3781 caaccttggt gaccttggtg tcgtcgccct tattgaccga gacatactcg ccgatcttcg
     3841 ctgcaagggc gtctgcatcc ttgacacagt ctaccttgac tgcccttcca agaatctggt
     3901 tgttctttcc gcccattgct ccggtcttga tcgagttgtc aaggccggcc aggttcaccg
     3961 cgactgtcct cttggtgagg tcgatgatct tcaggatacc cgcgttgttc agcgggctga
     4021 tcttctcaag ggcaacaccg ctcttgagca gcttcccctg atcgtcgtaa agatcacagg
     4081 tgtctttgta ttttgccata actttatcct ctgtaatatt ggttttgcca caatgcattt
     4141 cgataactga acggcaaagc aagctaggta gtttgctgat gatagatgtc ggtgactatt
     4201 gcacgtcccc tcacgggaca atctactata ctatatcacc cgatataaga tttccctttt
     4261 gtaatgaaaa tttgacattt atattaatac ttgtataatc taaaaaaaca ggggaatgtt
     4321 gatataccat atacattctt cataaatgcc acgtctttag tgctacgaag attgaatgag
     4381 atattacttt atcatggtta aatctttaaa aatttttaaa aactggtgcg ctgcttgttt
     4441 aaaaagggat cagatcttct ggtacagttt cccatacacg acctgacctt tccgcttggg
     4501 atgacgctca ccaagatcac catggaacag ggcgaacgtt gccttggtgc catcgacctc
     4561 aatttcttca tcctttacat atttgaagcc aggcatcatg acatcgattc caccaaagac
     4621 aatgatctcg ccttccacca tctgcccgcc gatcttgctc tttgcatctc ctttgatgac
     4681 gaccttcccg ccttctgcat gagtggcgat gtgtacgtcg gcattgcctt ccacaatgat
     4741 ggtgccgccg ttcatgaagt aaccgatgtc actcccggcg ttccccttca cccgtatcag
     4801 gccgccctgc atccctcgcc agtcgccccg gtaagcggct ccaaggtagt tgccggcatt
     4861 gccttcgatg atcagctctc cgcctttcat cccgatacct gcaaaagcat cgacattgcc
     4921 ctttgcagtg agcttgccgc cctgcatcca ggcaccgaca tacatgtcag catccttttc
     4981 aatgatcact tcgccggcag acatcttcat gccgatgtat tttacccggc tgacatctcc
     5041 cttgacaatg atcctggtat cggctgcggt tgcccccgca tcgcccgaga catcgaaata
     5101 tttgccgagg gtatactgct ccctgccttc ataggcatgg agggttgtga tctcggcagc
     5161 cttctttcct gcaaatgcat cgggggagat gttgtctgct tcaaggaaga gttcaggctg
     5221 tgtctttatg gttacggtaa cagtctccat ttttgcctca ccctcaccgt gtcgcgtcga
     5281 cctcgatcac tcgcggattt cccgagagga aatgcttg
//
LOCUS       DAOOCO010000139         5306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14579_length_5306_cov_4.928775, whole genome shotgun sequence.
ACCESSION   DAOOCO010000139 DAOOCO010000000
VERSION     DAOOCO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14579_length_5306_cov_4.928775"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK154_07980"
     CDS             complement(<1..309)
                     /locus_tag="PK154_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPW11034.1"
                     /translation="MEYRFARRMGCLPPSFLGELFRVSSQPWIISFAGGLPSSGLIDA
                     DGLSRATRQVIEEEGRMPFQYSTTDGYLPLREYIADRYRRRLGIPAEADEIQIVNGSQ
                     Q"
     gene            504..1118
                     /locus_tag="PK154_07985"
     CDS             504..1118
                     /locus_tag="PK154_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPW11035.1"
                     /translation="MDYFGLMKGFLLSPVETFRSVRETDLGESLKYYLVLLVINAILS
                     AIVGIAMVSAVWATFAGLSESLGLPFPAVAGFGLVVFAIVMIIVQFIFAFIIAAWLHL
                     FVYLLGGRKGYLQTLKSVTFGSTPAMLLGWIPFIGFIFGIWTIILEILGIRELHEMTT
                     GKAALAIILAILVIAIIIIAIAALFFIAFSDIVMEMSPRQITGI"
     gene            1407..2021
                     /locus_tag="PK154_07990"
     CDS             1407..2021
                     /locus_tag="PK154_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPW11036.1"
                     /translation="MERNIAPFDDVVAFHGHACPGLAIGYRAACAALQALSVERPVDE
                     ELVAIVENDACGIDAIQYVAGTTMGKGNLIFRDYGKRAWTFINRRTGKAIRVAEKPDF
                     FEKKSDPRLAELRRTVMAGTATRDEQQEYSRLSERYIRDLLSYPAEEMFTIREVAVEV
                     PEHARIFRSVTCASCGESVSESRARVKDGKIVCIPCHDHYTRGW"
     gene            complement(2310..2651)
                     /locus_tag="PK154_07995"
     CDS             complement(2310..2651)
                     /locus_tag="PK154_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW11037.1"
                     /translation="MTQSDDDALKVIQSKAEGVLQSDLWKILDVDSRKCSRIVKKLLD
                     SGMIERIEFRKDGIKTFVLKAKRRPVDPNQLLAGEELIPCIGCDMECVVIECPRLLDW
                     MYQLAICGVGE"
     gene            complement(2658..2894)
                     /locus_tag="PK154_08000"
     CDS             complement(2658..2894)
                     /locus_tag="PK154_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoprotein"
                     /protein_id="HPW11038.1"
                     /translation="MVNGIVLPIKKVFSLVDTKILVEIKDDNRKLQGRLVAVDEYLNI
                     HMDETTELVDGQRGRNLGTVVIRGNNILSISPSS"
     gene            complement(3015..4115)
                     /gene="fbp"
                     /locus_tag="PK154_08005"
     CDS             complement(3015..4115)
                     /gene="fbp"
                     /locus_tag="PK154_08005"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HPW11039.1"
                     /translation="MSKKTTISVIKADIGSFPGHSRTHPLILEKAAKMLKEEEGKLLI
                     DSFVTHCGDDTELIMTHTRGEDDEKIHALAWKVFMECTKMAREMKLYGAGQDMLSDSF
                     SGNVKGMGPGVAEMEFEERGSDPVLVFMADKTEPGAWNFFLYKMFADPFNTPGLVIDP
                     NMHGGFIFEVHDVMEKRRIQFRTPEETYSLLAYIGAPSRYVIKYVWRKDGLIGASTST
                     QRLSLIAGKYVGKDDPVMIVRGQNGLPAVGEIIEPFATPLIVAGWMRGSHHGPFMPVG
                     LPDANCTRFDGPPRVLCLGFQICNGKLIGPADMFDDPAFDRVRDKCNALADILRAHGP
                     FEPHRLSLDEMEYTTLPAVEKQLKDRWEPIPE"
     gene            complement(4206..4625)
                     /locus_tag="PK154_08010"
     CDS             complement(4206..4625)
                     /locus_tag="PK154_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11040.1"
                     /translation="MNGIPFDEKHLEELIRQIMEQSGDGRPLIIGMKIVIAGPPGGTP
                     PLSRGDTTEPEIELHRIGDRVVLTTELPGITPEEVQVLFREDRVFIWAKNPDRQYRAS
                     REVPPAIKGSAEITFRHGVLEVSYLPREAGSEEESAK"
     gene            complement(4631..>5306)
                     /locus_tag="PK154_08015"
     CDS             complement(4631..>5306)
                     /locus_tag="PK154_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW11041.1"
                     /translation="HVTESVVSQILTELDGLEELKNVTVIGATNRPDMLDPALMRPGR
                     MERHIYVPPPDAESRKKIFEVYLKDAGALLTDDVRIDDLIAETEHYVGADIEALVREA
                     KLAAMREFITTMAGKSPEELSDAAGNIRLTRKHFTEAAGKVKASLDAEALEAAERQAW
                     EMLYSGDQKEILDAAMGAVRMAELRKEHRDETQKLRALVFARSKDWNEIKQRTLELSG
                     KAKLTA"
BASE COUNT         1007 a         1587 c         1550 g         1162 t
ORIGIN      
        1 ctgctgggac ccgttcacga tctggatctc gtcagcctct gcggggatcc cgaggcggcg
       61 ccggtaccgg tcggcgatgt actccctgag cggcaggtac ccgtcagtcg tgctgtactg
      121 gaagggcatc ctcccctcct cttcgatcac ctggcgggtc gcccgggaga gcccgtcggc
      181 gtcaatgagc ccggaggagg ggagcccccc ggcaaacgag atgatccagg gctgtgacga
      241 gacgcggaac agttccccca gaaatgacgg gggtaggcac cccatccgcc gtgcgaaacg
      301 atactccatc cggctgcacc tgcagtgata tgggggacga tgagccaaaa agggcacggg
      361 atcaggacac tccccctctg tgccgcagga gtggttggca gggacccgaa cggtaatccg
      421 ggcagttgaa gggaaaaaac cgggaaatat ccagtatttc aaaagattta aatcgcatca
      481 tggtgagagt tttcccgata cacatggatt atttcggtct gatgaaaggg ttcctcctct
      541 ctcccgtgga gacgttccgg tcggtgaggg agacggatct cggggaatcc ctcaaatatt
      601 acctggtcct gctcgtgatc aacgccatcc tctccgcgat cgtcggtatc gccatggtga
      661 gtgcggtatg ggcaacgttt gcaggactct ccgagtcgct gggcctcccc ttccctgccg
      721 tggcagggtt cgggctggtc gtctttgcga tcgtgatgat catcgtccag ttcatcttcg
      781 cgttcatcat cgccgcatgg ctccacctgt tcgtctatct cctcggcggc agaaagggat
      841 atctccagac gctcaagtcg gtgacgttcg gctccacccc tgcgatgctc ctcggctgga
      901 tccccttcat cgggttcatt ttcgggatct ggaccatcat ccttgagatc ctcgggatcc
      961 gggagctcca cgagatgaca accggaaagg ctgctcttgc catcatcctt gccatcctcg
     1021 tgattgcaat catcatcatc gccatcgcag ccctgttctt catcgccttc tctgatatcg
     1081 tgatggagat gtcaccgaga cagatcaccg gcatctgatc cttttttctc acttctgcga
     1141 aacctccttt tttctgaagc aagaaagcga tcgcttctgc cggccaggtc ctgtaatcag
     1201 gcagtgacgt gatcaccccc tgtaccatca tgttttgggg aaccgggtct gctgcagtcc
     1261 ggatcatttc gctcgatcgt ccggccagca cccatccacc ctttttttgg ggaaccgggt
     1321 ctgctgcagt ccggatcatt tcgctcgatc gtccggcctt gcacccatcc accctttttt
     1381 tggggaatcg ggtcccatga gctggtatgg aacggaacat tgcccccttc gatgacgttg
     1441 tcgccttcca tgggcatgcc tgcccggggc ttgcgatcgg gtaccgggca gcctgtgcag
     1501 cgctgcaggc gctctccgtg gaacggcccg tcgacgagga gctcgtggct atcgtcgaga
     1561 acgatgcctg cgggatcgat gccatccagt atgttgccgg cacgacgatg gggaagggaa
     1621 acctgatctt ccgggactac gggaagcggg cctggacctt catcaaccgg cggaccggaa
     1681 aggccatccg ggtcgcagag aagccggact tctttgagaa gaaatcggat ccgcggcttg
     1741 cggagctgag aagaacggtg atggcaggga cagcgacacg tgatgaacag caggagtatt
     1801 cccggctgag cgaacgctac atccgggacc tcctctccta ccctgccgaa gagatgttca
     1861 ccatccggga ggtggccgtt gaggtacccg agcatgcccg gatcttccgg tcggtcactt
     1921 gtgcatcctg cggggaatcg gtctccgaat cccgggcacg ggtgaaagat gggaagatcg
     1981 tctgcatccc ctgccatgac cactataccc gtggctggtg aggagtgaag gggaggatct
     2041 gatctttttt atgcagaaat cttcattgaa gggaccgtac atacggacgc tcccgggggt
     2101 tgcgcctccg gcttagcccc gccgctgtgg cgccgcccca gatgcgatag gggaggaaaa
     2161 gaatgcgatt cattctctga ctgagagtat acctcttctt cgaaagttcc ggaactgttg
     2221 aacccgtgtt tgtttcagaa gcctatggat tgaaaaaata agataaaaaa aggattctat
     2281 cagataaaaa tccggagaaa aaggcccggt tactcgccga caccgcagat ggcgagctgg
     2341 tacatccagt caaggagccg cgggcactcg atcaccacgc actccatgtc gcagccgatg
     2401 caggggatca gttcctcgcc ggcaagcagc tggttcgggt cgaccgggcg cctctttgcc
     2461 ttcagtacga aggtcttgat cccgtctttc cggaactcga tccgctcgat catgccggaa
     2521 tcgaggagct ttttgacgat cctcgaacat ttccgactgt cgacatcgag gatcttccag
     2581 aggtcactct ggaggacccc ctctgccttg gactggatca ccttcagggc gtcatcatcg
     2641 gactgggtca tggtccttca ggagctcggg gagatagaga ggatgttgtt cccgcggatc
     2701 acgaccgtcc ccaggttcct gccccgctgt ccatcgacga gctcggtcgt ctcgtccatg
     2761 tggatgttca ggtactcatc caccgcgacc agccgtccct gcagcttcct gttgtcgtcc
     2821 ttaatctcga caaggatctt cgtatccacg agagagaaga ccttctttat tggcagcaca
     2881 atcccgttaa ccatctgcca ttctatgggg atgtgaggat attaaaggtt ccccaaatgg
     2941 tacagggagg agcggagcga tccgtttccg gtggaaaaaa gcgatcccga aaagaccggg
     3001 aaaaagggtg atacttattc ggggatcggt tcccagcggt ccttgagctg cttttccact
     3061 gccggcaggg tcgtgtattc catctcgtcc agggagagcc ggtggggctc gaacgggcca
     3121 tgggcacgga ggatatcggc aagggcgttg cacttgtcac ggacacggtc gaaggccggg
     3181 tcatcgaaca tgtcggccgg gccgatcagc tttccgttgc agatctggaa cccgaggcag
     3241 aggacccggg gagggccgtc aaaccgggtg cagttggcat ccgggagccc caccggcatg
     3301 aacggcccgt ggtgggagcc ccgcatccag ccggccacga taaggggggt tgcaaacggc
     3361 tcgatgatct ctccaacggc aggcaggccg ttctgtccgc ggacgatcat aacaggatcg
     3421 tcttttccga cgtattttcc ggcgatcagg ctgagccgct gcgtgctcgt ggaggcaccg
     3481 atcaggccat cttttcgcca gacgtacttt atcacgtacc gggagggtgc cccaatgtag
     3541 gcgagcaggc tgtaggtctc ttccggggtg cggaactgga tccggcgttt ctccatgacg
     3601 tcatggactt cgaagatgaa cccgccgtgc atgttcgggt cgatgacaag gccgggagta
     3661 ttgaacgggt cggcaaacat cttgtagagg aagaagttcc aggcccccgg ctcggtcttg
     3721 tcggccatga acacgaggac cggatcggaa ccgcgttcct caaactccat ctccgcgacg
     3781 ccaggcccca tccctttcac gttgccggag aacgagtcgg agagcatgtc ctggccggca
     3841 ccatagagtt tcatctcccg ggccattttg gtgcattcca tgaagacctt ccaggcaagg
     3901 gcatggatct tctcgtcgtc ctcccccctg gtatgggtca tgatcagctc ggtatcgtcc
     3961 ccgcagtgcg tgacaaagga gtcgatcagg agcttcccct cctcctcctt cagcatcttt
     4021 gctgccttct cgaggatcag gggatgggtc cgtgagtgac ccggaaagga tccaatgtct
     4081 gcttttatca ccgatatcgt cgtcttcttt gacatcaaaa ccactcacta atacagttgc
     4141 tccaccagga taaaagatat ggtggttctt ctgatcccag ggggatcagg cccgccatgg
     4201 agggattact tggcggactc ttcttctgaa ccggcttccc gcgggaggta ggagacctcg
     4261 agcaccccgt gccggaaggt gatctctgca ctccccttga tcgccggcgg cacttcgcgg
     4321 ctggcacggt actggcggtc agggttcttg gcccagataa agacccggtc ctcccggaag
     4381 aggacctgga cctcctctgg ggtgatgccg ggcagctccg tagtgagcac gacccggtcc
     4441 ccgatccggt gcagctcgat ctccggctcg gtggtatccc ctctcgagag ggggggggtc
     4501 ccgccaggcg gccccgcaat cacgatcttc atcccgatga taagcggccg gccgtccccg
     4561 gactgctcca tgatctgcct gatcagttcc tcgagatgtt tctcgtcgaa gggtatcccg
     4621 ttcatcgtag ttacgctgtc agcttcgctt tcccggagag ctcaagcgtt ctctgcttga
     4681 tctcgttcca gtccttgctc cgggcaaaga ccagggcacg gagcttctgg gtctcatcgc
     4741 ggtgctcctt ccggagttcc gccatccgga ccgcccccat ggcagcatcg aggatctctt
     4801 tctgatcacc cgaatagagc atctcccagg cctgccgttc ggcagcctcg agtgcctcgg
     4861 cgtcaagcga agccttcacc ttcccggcag cctcggtgaa gtgcttccgg gtgagccgga
     4921 tgtttccggc tgcatcggac agctcttccg ggctctttcc tgccatcgtg gtgatgaact
     4981 cacgcatcgc cgcaagcttc gcttcgcgga cgagagcctc gatgtccgca cccacatagt
     5041 gctcggtctc cgcaataaga tcatcgatcc tgacatcgtc ggtgagcagc gccccggcat
     5101 ccttcaggta cacctcgaag atcttcttcc tgctctcggc atcagggggg gggacataga
     5161 tgtgccgctc catccggcct ggccgcatca gggcggggtc gagcatgtcc ggccggttcg
     5221 tggccccgat caccgtgacg ttcttcagct cctccagccc gtcgagctcg gtcaggatct
     5281 ggctgaccac gctctcggtc acgtgc
//
LOCUS       DAOOCO010000140         5291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14640_length_5291_cov_3.707792, whole genome shotgun sequence.
ACCESSION   DAOOCO010000140 DAOOCO010000000
VERSION     DAOOCO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_5291_cov_3.707792"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..879
                     /gene="fdhD"
                     /locus_tag="PK154_08020"
     CDS             34..879
                     /gene="fdhD"
                     /locus_tag="PK154_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPW11042.1"
                     /translation="MAEDTPTAGKAGGGSGKKKPPRLFRKVPCTRITDEQVEEILHEV
                     IEETPYALFVNGRHMMTAMMTPADLEDFVTGFLFTEQVIKGPEEIESIRVEKNRISVI
                     TTNLFRVVGPKKTILSGCGGSTSYIDSEKLPNIRSDFSVGSGGIADLIKQTLTSELHQ
                     LTGGIHVVALAGAEGIITVSEDIGRHNALDRVIGYGLRNGIDFSRTFILVSGRVSSEM
                     ARKCLIANIPIIASRGATTTLALEVAVKNGLTIVGFVRGSKMNVYTHTKRIKITGRQA
                     SSGST"
     gene            complement(909..1676)
                     /locus_tag="PK154_08025"
     CDS             complement(909..1676)
                     /locus_tag="PK154_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HPW11043.1"
                     /translation="MAVALSGGKDSTALLLVLATILPEVPDVSLTALTIDEGIAGYRE
                     ETVDSAVRLTRHLGVSHHIISFPDLFGADLDTLLQKKGRAERACTVCGVLRRKALAEG
                     ARRVGGTTIATGHNLDDEAQSVLMNLLRGDLVRLIQDSSSGEPACFLPRIKPLAGITE
                     KEVVTFLFVQGYFTELPECPYAGTALRSEIRTMLGDLEFRHPGTRERLMKWQERVRER
                     VTPRSIAGDLTRCTRCGALCNSEICQVCRILESLGIE"
     gene            1701..2081
                     /locus_tag="PK154_08030"
     CDS             1701..2081
                     /locus_tag="PK154_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11044.1"
                     /translation="MIPDILPDTSLNGGDQVTGTEPFPGVSSKDDRRFLAGRASIGGN
                     RSFPRGSQDLPPMGNADDPDLVVSGGRDIFGYQLLILQDDDTCGEYAEVREDGRDGHG
                     IARPCDECFVREDNLHALLFWFAP"
     gene            2230..3900
                     /locus_tag="PK154_08035"
     CDS             2230..3900
                     /locus_tag="PK154_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW11045.1"
                     /translation="MRRPVYWIVITLLLCVVLGCASAEGGNGGASAPGLQEYSSGQST
                     ESPAPGPDSPVQEAVSFGVSGTGTGYPDDPGDLQGTSMADPGLEEAGSSTIAMGADST
                     GIGPGYQGDFSAGPGDAPSALTGGGPGDAGSGSDDHGFFSSSEDHGPFSSSGDSPGEG
                     YQEGSGGASSPGPSFMGGEDPVLPDTGDQDGTPSPGRGNGGLLQNRGSGTEPETGILE
                     GHGGESGPKLPPNALARSGPGDGGSVVPGQLHGSLGILAASAGGAGVAWAGPEGSGEH
                     SASGPSSRAHGPHRQQQGIPVQYPCGPAQATPVIPDQGSKATDTREDTGQRARSKRAR
                     VYFPEEGSVQSPVSAPPSSPIPLFPFSLLLFGGYRRISKKNVLEHGARQSVFSAISAH
                     PGIDVPLLAGTTGINENTLRYHLVKLVESGRITYLVKPGVIRYFLNQGSYSPAEQVLI
                     HYLWSGTPRNILLLLRSKPGLSRQQISDMLGISGPSVTRHMEHLIDDGIIENRFFGRS
                     NHYYLTEGTKAIFEPMLSRIRAATEDGESPLVLPTPGEGPLIEPSLVS"
     gene            4057..4629
                     /locus_tag="PK154_08040"
     CDS             4057..4629
                     /locus_tag="PK154_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11046.1"
                     /translation="MIKKIVLCVVLVMVLVPAFAMAAGPQGQGSGTAQGNYQQFQQQT
                     ATQEQVANQVQNVYQEQVANQEQTASQLQNNGEEQMLQNRICSQECEMSQMMKQNQSQ
                     VRKGSALGLGTGAGVGDQRQNQTRNMFGEMIQNRTRLQDGSCGNCPTLNTGLTASADQ
                     LQDQTRDMTWDRTRGRLHLQDGSCGNCLNS"
     gene            complement(5060..>5291)
                     /locus_tag="PK154_08045"
     CDS             complement(5060..>5291)
                     /locus_tag="PK154_08045"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPW11047.1"
                     /translation="FTDPVLQNLHDDLIARGSASPVEALKAGVLVEETDIADLNKALV
                     TTEKNDIRTVYNNLLQGSMNHLNAFQSNLASY"
BASE COUNT         1138 a         1486 c         1587 g         1080 t
ORIGIN      
        1 ccataaagga aaaaatggat gtggtgatga gacgtggctg aagatacccc aacagcggga
       61 aaggcaggtg gtggatctgg gaaaaagaag cccccgcggt tgttcaggaa ggtgccctgc
      121 acccggatca ccgatgagca ggtcgaggag atcctccacg aggtcatcga ggagactccc
      181 tatgccctct tcgtgaacgg gcggcacatg atgacggcga tgatgacccc ggcagatctt
      241 gaggacttcg tcaccgggtt cctcttcacc gagcaggtga tcaagggtcc cgaagagatc
      301 gagtcgatca gggtagaaaa gaaccggatc agcgtgatca ccacgaacct gttccgcgtg
      361 gtcgggccta aaaagacgat cctctcgggg tgtggcggga gcacctctta catcgactca
      421 gaaaagctcc cgaacatccg gtccgatttt tccgttgggt cagggggtat cgcagacctg
      481 atcaagcaga ccctcacttc tgaactccac cagctgaccg ggggtatcca tgtcgtcgcc
      541 ctggccggtg cggaaggcat catcacggtc tccgaggata tcgggaggca taacgccctt
      601 gaccgggtca tcgggtatgg attgcgaaac gggattgatt tttcccggac cttcatcctc
      661 gtctccggcc gggtctcgtc agagatggca cgaaaatgcc tcattgcaaa catcccgatc
      721 atcgcctccc ggggtgcaac caccaccctc gcactggagg ttgccgtgaa gaacgggctg
      781 accatcgtcg gatttgtgcg gggatcgaag atgaacgtct atacccacac gaaacggatc
      841 aaaataaccg gccggcaggc atcgtcagga tccacttgat cctggatatg gtacaagaca
      901 tttcgggatc attcgatccc gagtgattcc aggatcctgc acacctggca tatctcgctg
      961 ttgcagagcg ccccgcaccg ggtgcagcgg gtgagatctc cagcaatgga tcggggagta
     1021 acccgttccc tgaccctctc ctgccacttc atcagcctct cacgtgtccc cgggtgccgg
     1081 aactcaaggt cgccaagcat cgtccggatc tcggaccgga gggctgtacc ggcgtacggg
     1141 cattcgggca gctcggtaaa atacccctgg acaaacagga aggtgacaac ctctttttca
     1201 gtgattcctg ccagcggctt gatcctcggc aggaaacagg ccggttcccc ggacgagctg
     1261 tcctgtatca gccggaccag gtcgccccgg aggagattca tcaggaccga ctgggcctcg
     1321 tcatcgaggt tatgcccggt ggcgatcgtg gtccctccca cccgccgggc tccttctgcg
     1381 agggcttttc gccgaaggac tccacagacc gtgcaggccc gctcagcacg ccccttcttc
     1441 tgcaggaggg tgtcaagatc cgctccgaag agatcaggga atgagatgat atggtggctg
     1501 acacccaggt gcctcgtgag gcggaccgcc gagtcgaccg tctcctcgcg gtatcctgcg
     1561 atgccttcat cgatggtaag agccgtgagc gagacatccg gcacttccgg gaggatcgtt
     1621 gcaaggacga ggaggagtgc ggtactatct ttcccgccgc tgagcgcgac ggcgatatgg
     1681 tcccccgggg agatgaggga gtgatccctg atatactccc tgacacgtct ctcaatggag
     1741 gagatcaggt gaccggcaca gagccgttcc ctggggtctc ttccaaggat gaccgcaggt
     1801 tccttgcagg aagagcatcg atcggcggca accgttcttt tcccagaggt agtcaggatt
     1861 taccccccat gggcaatgcg gatgatccgg acctggtcgt ctccggaggc cgtgacatct
     1921 tcgggtacca gcttctcatt cttcaggacg atgacacttg cggggagtat gccgaggtcc
     1981 gcgaggatgg ccgcgatggc cacgggatcg cccggccgtg tgacgagtgt tttgtccggg
     2041 aggataacct gcatgctctg ctcttctggt ttgctccctg atcacatata tcccatggag
     2101 caggtctgca tccgtcacct ctcgccttca gtgccgggta gaaccgcggt ttgtttgtaa
     2161 acgataccat cacctgtagc tgccggaatg agattccttt taagaagcgc cttcgcatag
     2221 tgtagtaaaa tgagacggcc ggtatactgg atagtcatta ccctcctcct ctgcgtggtc
     2281 cttgggtgtg cctcagcaga agggggaaat ggcggggcat cagcgcctgg tctccaggag
     2341 tactccagcg gccaatcaac ggaaagcccg gcaccaggac ccgatagccc ggtgcaggag
     2401 gcggtatctt tcggagtatc aggcaccgga acgggatacc ctgatgatcc gggagatctc
     2461 caagggacca gcatggctga tccggggttg gaagaggcag gcagctcgac catcgcaatg
     2521 ggagcagatt cgaccggtat tggacctggg tatcagggag atttttcagc aggacctggt
     2581 gatgctccca gtgccctgac cggaggcgga cctggcgatg cgggtagcgg atcggacgat
     2641 catggcttct tctcttcttc ggaggatcac gggcccttct cttcttccgg ggattcccct
     2701 ggggagggat accaggaggg ttccggaggg gcatcctccc ccggtcccag cttcatgggt
     2761 ggagaggatc ccgtactccc tgataccggt gaccaggacg ggaccccgtc tcctggaaga
     2821 gggaatggcg gcctccttca gaacagaggt tccggcaccg agccagagac cggtatcctg
     2881 gaggggcatg gaggagagag cggaccaaaa ctccccccaa acgccctggc gaggtctggt
     2941 ccgggtgacg gaggcagcgt ggttcccggg cagctgcatg gatcgcttgg tattcttgcc
     3001 gcctctgcgg gaggtgcggg ggtggcctgg gcaggacccg aaggttccgg ggagcattca
     3061 gcatctgggc cctcgtcaag ggcccatgga cctcaccggc agcagcaggg gatcccagtc
     3121 cagtacccct gcggccctgc ccaggccacc ccggttatcc ctgaccaggg gtcgaaggcg
     3181 accgatacca gggaggatac aggccaaaga gcccgatcga aacgggcacg ggtgtacttc
     3241 ccggaagagg gttccgttca atcccctgtc agcgctcctc cctcctcccc cataccgctc
     3301 tttcccttca gcctgctgct cttcggaggg tatcgaagga tctcgaagaa gaacgtgctc
     3361 gagcatggcg cccgtcagag tgtcttttct gcgatctccg cacacccggg cattgatgtg
     3421 cccctgctgg caggaactac cgggatcaat gaaaacacgc tccggtacca tctcgtaaaa
     3481 ctggtcgaga gcgggaggat cacctacctg gtcaagcccg gagtgatccg gtacttcctc
     3541 aaccagggat cgtattcacc cgccgagcag gtcctgattc attacctctg gagcgggacg
     3601 ccgcggaaca tccttcttct gctccgctcc aaaccgggat tgagccgaca acagatctcc
     3661 gatatgctgg gaatatccgg tccctcagtg acccgtcaca tggagcacct tatcgatgac
     3721 gggatcatcg agaaccggtt tttcgggaga tcgaaccatt attatctgac ggaggggaca
     3781 aaggcgatct tcgaaccgat gctctcccgt attcgtgctg caacagaaga tggcgaatcc
     3841 ccccttgtgc ttcccacacc gggagaagga cccttgatcg agccttctct cgtgtcttaa
     3901 ttctggcagc ccttccctga aagcggtggg gctgctgcct ctcccttgtg tagcttcccc
     3961 tttgagaaaa ggtttgatag cccctgccga ttgactgatg gagacgggtc atggggaatg
     4021 gttgtcccgg acccggctaa ccaggagaag acagatatga taaagaagat agtgctctgt
     4081 gtggtcctgg tcatggtcct tgtaccggcg tttgctatgg cagccgggcc gcagggccag
     4141 ggatcaggaa ctgcccaggg caattaccag cagttccagc agcagactgc tacccaggag
     4201 caggtcgcaa accaggtgca gaatgtatac caggaacagg tcgcaaatca ggagcagacc
     4261 gccagccagc tacagaataa cggggaggaa cagatgctcc agaaccgcat ctgcagccag
     4321 gaatgcgaaa tgtctcagat gatgaagcaa aaccagagcc aggtgcggaa aggatctgca
     4381 cttggtctgg ggaccggtgc aggagtgggt gaccagcggc agaaccagac ccggaacatg
     4441 ttcggggaga tgatccagaa ccggaccagg ctccaggatg gatcctgcgg gaactgcccc
     4501 accctgaata caggacttac agcatctgct gatcagctgc aggaccagac ccgggacatg
     4561 acctgggaca ggacccgggg ccggctccat ctccaggatg gatcctgcgg gaactgcctg
     4621 aatagttgaa gatcggtccg gatgcatcaa tcggtgcatt cccggatgac cttatttttg
     4681 atgtttgaag atctcgtccg ggtatcaccc gttcgtgggc gtgtggtgtt ccttccctct
     4741 tcgcctgaca tacatccatt ctcaccagca tgtcaggggt cccgggaaaa taatgatgca
     4801 gtttgggata gtatcaattg ctggcctcat cagggagcat tccagccttc ccccacaggg
     4861 cagggaaccg tgttggaacc gggtaagggt gttcttgtga tgaaaacaat aatatgattc
     4921 tttgagggcc acaagcataa aagatgaaag tgccggaggc agtatcatat aaacctccta
     4981 tgagccccga tttcgggata cgggcataaa ttatgaaaat tacgaagtga ttttcattgg
     5041 aaaaagcagg gtgagtggtt cagtagctcg ccaggttcga ttgaaaagca ttcagatggt
     5101 tcatggatcc ctgcagaagg ttgttgtaca cggtcctgat atcattcttc tctgttgtga
     5161 caagagcctt gttgaggtcg gcaatatcgg tctcctcgac gagtactcct gccttgagtg
     5221 cctcgaccgg agaggcactt ccccgggcga tcaggtcatc gtggaggttc tgaagaacgg
     5281 ggtccgtgaa c
//
LOCUS       DAOOCO010000141         5271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14712_length_5271_cov_4.456863, whole genome shotgun sequence.
ACCESSION   DAOOCO010000141 DAOOCO010000000
VERSION     DAOOCO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14712_length_5271_cov_4.456863"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK154_08050"
     CDS             complement(<1..961)
                     /locus_tag="PK154_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW11048.1"
                     /translation="MDEYPFLIGGEWRTGAIEPLEICFPYTGEPFAKVHRAIEQDCRD
                     AVSCVAGSSRSARILSSGERSRILSELADLVKVNTPEFVKILILEGGKTRMVAEQEVA
                     RACITLRISAEEARRITGEFLPLDWSPDTEGRFGITRRVSLGPALAIIPFNYPLNLAC
                     HKIGPAIASGTPLVLKPASVTPVSSLMLGRLALKAGLPPDMLSVVPCSAATAEAMVRD
                     PRIALLTFTGSPEVGWHLKEIAGRKKVLLELGGNAAVIVHSDAQLPYAVSRIVSGGFT
                     NAGQVCISVQRVYIEQSIYRSVVAMILDRITALKTGDPRDADTD"
     gene            1089..4307
                     /gene="ileS"
                     /locus_tag="PK154_08055"
     CDS             1089..4307
                     /gene="ileS"
                     /locus_tag="PK154_08055"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPW11049.1"
                     /translation="MKEVTSSYHANEIEESIRQFWLEEAIVEKTRELHLSDPDFFFVD
                     GPPYTTGNIHLGTAWNKIIKDSILRYTRMTGRNVIARAGYDMHGLPIEVRVEQQLGFV
                     SKKDIEQFGIGNFIEKCRTFAAANMEVMSRQFEALGIWLDFADPYQTMKPEYIEAAWW
                     TLARADEEGMLERGHRVVNWCPRCATAIADSEVDYADVTDPSIYVKFPLTGKDHEYLV
                     IWTTTPWTLPANVAVAVHPDIVYARVEAVKDGAKEILWMAEDLVDSVLRKGRYQDYRL
                     IEKINGVELTGLTYSSPIEDTVTLQRTLAHRVVTADFVTLENTGMVHIAPGHGWDDYL
                     LGCREDLPIICPVDASGRFTREAGTLEGMFIKEADPVVLHDLGHHLLARESVTHRYGH
                     CWRCKTPIIFRATEQWFLKASAIRDRMLDEVGKVLWYPEWAGSARFYDWIKEARDWCI
                     SRQRYWGIPIPIWECKSCGAHRVFGTFSDLEEASGRSITDPHRPFVDEIAVSCKCGGE
                     MRRVEDIFDVWFDSAVASWATLRFPGEREAFERLWPADFITEGQDQTRGWFYSQLGAS
                     TIAFGCSPYRSVLMHGFALDAEGRKMSKSLGNVVTPDEVVTTQGVDVLRLYVLSSSAP
                     WDDLKFNWEGVKTVNRAINIYWNVYRFPLPYMILDHYEPYTTADGTWDDDALRSGLGS
                     MAPEDRWIISRINSCALIVSKAMMERQLHRATRALIQFILEDLSRWYVQLVRPRMWLE
                     GTSEAKATAYETMYYVIRRLCGLLSPFIPHITEMMYQNIRTLSDPPSIHMTDWWGGDE
                     SLVDTVMEHSMDIVRSFDEASANARQAGKRKLRWPVSEIVVVTDDDDVIAALESLSSI
                     CKDRANARAVRVVRGAYDRIGWKAEPVMKALGPAFGKNAPIVRELIRSADAAALKSPL
                     DKGETVTLAGSDGVYPIQPGYVAFLEELPPNLFQAHMERGTVYVDVTLTADLEAEGYA
                     REVIRRIQEMRRQRDLNVEDYITAGIIIDDERVCSLLQGRWEDVIQEEVRAGSLSISP
                     GSSVSMNGSFELERDWDIEGIQAVIGISKFTDQTGKKQTAFR"
     gene            complement(4260..4991)
                     /locus_tag="PK154_08060"
     CDS             complement(4260..4991)
                     /locus_tag="PK154_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HPW11050.1"
                     /translation="MKRREIFSNLLSVPPVFVRLDGRGFHRVAEEWGLTRPFDSRFAR
                     AMARVSERLITSSGLSPDLAFTFSDEINLYFSHVPYSGRVEKIDSITASYAASALTIE
                     MSSPFPVAFDARIIQVSPLYALQYLIDRQAEAWRNHSNSYCHQTLVGEGKASHEAAQL
                     LKGLSSSELHEMMFSRGLNLAKTPAWQRRGIFVYKTAKEIEGFNPVEKKMVKTIRTSV
                     KCISEPPVFSSPEGRLFLSGLIREL"
     gene            5047..>5271
                     /locus_tag="PK154_08065"
     CDS             5047..>5271
                     /locus_tag="PK154_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11051.1"
                     /translation="MLEPRFLIPVFDKILPPGHVLSLSYVGPFSRKASQKNRIGFSLK
                     GRIRILAGYQISSALKAPGRWRMRTMSPMLL"
BASE COUNT         1236 a         1378 c         1474 g         1183 t
ORIGIN      
        1 cgtctgtatc cgcatctctc gggtcgcctg ttttcagtgc agtgatccgg tcaaggatca
       61 ttgccaccac actccggtag atggactgct cgatatagac tctctggacc gatatgcaga
      121 cctgcccggc attggtaaat cccccgctta cgatacggga cacggcatag ggcaattgcg
      181 cgtcggaatg tacgatgacc gctgcatttc ccccgagttc gagaaggacc ttctttcttc
      241 ctgcaatctc cttcaggtgc cagcccacct cagggctgcc agtgaaggtg aggagcgcga
      301 tgcggggatc gcggaccatt gcttctgccg ttgccgctga acaggggaca acactcagca
      361 tatccggagg aaggccagcc tttagtgcaa gcctcccaag catcagcgaa gagactggcg
      421 tcaccgaagc cggcttcagc accaggggcg ttcccgatgc gatggcaggc ccgatcttat
      481 ggcacgcaag gttcagcgga taattgaacg ggatgatagc caatgcaggg ccaagagaga
      541 cgcgccttgt gatcccgaat ctcccctccg tgtcagggct ccagtcaagg ggaaggaatt
      601 ccccggtgat cctcctcgcc tcttctgccg atatgcgaag tgtgatgcac gcccttgcca
      661 cttcctgttc tgcaaccatg cgggtcttcc ctccttcaag gatcaggatc ttcacgaatt
      721 caggggtgtt aaccttcaca aggtcagcaa gttccgacag gatacggctc cgttccccgg
      781 aggagagaat acgtgcagaa cgggatgatc ctgcaacaca ggagaccgcg tcccggcaat
      841 cctgttcaat ggccctgtga actttcgcga aaggttctcc cgtgtagggg aaacagatct
      901 ccaggggttc gatggcaccg gtccgccatt ccccaccgat aagaaacgga tattcatcca
      961 tcccgatact cctaacagcg ctgcacatct ttgaagaaaa tactttgtgg tcgcggggac
     1021 aatatctctg gaatatacgc gcaggcagag gttgtcgcgg gtttgttaca agcgttcggg
     1081 ggatcccgtt gaaggaagtc accagcagtt accatgcaaa cgagattgag gaatcgatcc
     1141 gccagttctg gctggaagag gcgatcgttg aaaagacacg agaactccac ctttcagacc
     1201 ctgacttttt ctttgtagac gggcccccct atacaacggg taatatccat ctcggcacgg
     1261 cctggaacaa gatcatcaag gacagcattc tccgctatac caggatgacc gggagaaatg
     1321 ttattgcacg cgccggttat gacatgcacg ggcttcctat cgaggtgaga gtcgagcagc
     1381 agctcggatt tgtctcaaaa aaagatatcg agcagttcgg aatcgggaac tttatagaga
     1441 aatgccggac ttttgccgct gccaacatgg aagtgatgag caggcagttc gaagcgcttg
     1501 gcatctggct tgattttgct gatccctacc agacgatgaa gccggaatat atcgaggctg
     1561 catggtggac acttgcccgc gcagacgaag agggcatgct cgaacggggt caccgggtgg
     1621 tcaactggtg tccacggtgt gccaccgcta ttgctgactc tgaagtcgat tacgccgatg
     1681 tcaccgatcc atcgatctat gtcaagtttc ccctgaccgg aaaggaccat gagtaccttg
     1741 tcatctggac gaccaccccc tggactcttc ctgcgaatgt tgcggttgca gtccaccccg
     1801 acatcgtgta cgcccgtgtc gaggcggtca aggacggagc aaaggagatt ctctggatgg
     1861 ctgaggatct tgtcgattca gtgctccgga agggcagata tcaggactac cggctgatcg
     1921 aaaagatcaa cggtgtcgaa ctgacgggac tgacttattc gtccccaatt gaagatacgg
     1981 tgactctcca acgcactctc gcgcaccggg ttgtcacggc tgatttcgtc actcttgaaa
     2041 ataccgggat ggtgcacatt gcaccaggac atggatggga tgactacctt ctcggatgtc
     2101 gcgaggatct gccaatcatc tgtcctgtcg atgcctcagg gcgttttaca cgcgaagcag
     2161 ggaccctgga ggggatgttc atcaaggagg cagatcctgt cgtcctccat gatctcggtc
     2221 atcatctgct tgccagggag tccgtgaccc accgttatgg acactgctgg aggtgcaaaa
     2281 caccaatcat cttccgtgcc accgaacagt ggttcctgaa agcatccgca ataagggacc
     2341 ggatgctcga cgaggtcgga aaagtactct ggtacccaga atgggcaggg agtgcacgct
     2401 tttatgactg gatcaaggaa gcccgtgact ggtgtatttc tcgacagcgg tactggggca
     2461 tccccatccc gatatgggaa tgcaaatcct gcggggcaca ccgggtattt ggcactttca
     2521 gtgatctcga agaggcaagc gggagatcca tcactgatcc tcatcgtcct tttgtcgatg
     2581 agatagcggt ctcatgtaaa tgcggcggag aaatgcgcag ggtcgaggac atctttgacg
     2641 tatggtttga ttcggcagtc gcttcgtggg ctaccctcag gtttccggga gagagagagg
     2701 catttgaacg cctgtggccg gccgatttta tcaccgaggg gcaggatcag accagggggt
     2761 ggttctactc ccagcttggc gccagtacca ttgcattcgg atgctccccc tatcggagtg
     2821 tgctgatgca tggtttcgcc ctggatgctg aaggcaggaa gatgagtaaa agcctcggaa
     2881 atgtcgttac ccctgacgag gtggtgacga cacaaggggt tgatgtactg cgtctgtacg
     2941 tactctcttc aagtgctcca tgggacgatc tcaagttcaa ctgggagggg gtaaagacgg
     3001 tgaaccgggc gataaacatc tactggaatg tctaccggtt cccccttccc tacatgatcc
     3061 ttgaccatta tgaaccatat accactgcgg acgggacatg ggatgatgat gctctccgtt
     3121 cagggctggg ttccatggcg cccgaggatc ggtggataat ctcccggatc aattcctgtg
     3181 ctcttatcgt cagcaaagct atgatggagc gtcagctcca ccgggccacc cgggcactta
     3241 tccagttcat ccttgaagac ctctcccggt ggtatgtcca gttggtaagg cccagaatgt
     3301 ggcttgaggg aacctctgag gcaaaggcca cagcctacga gacgatgtac tatgttatcc
     3361 ggcgactttg cgggctcctt tcaccattta ttccccatat taccgagatg atgtaccaga
     3421 atatccggac attgtcagat ccaccgagca ttcacatgac cgactggtgg ggaggggacg
     3481 agagcctcgt tgacaccgtg atggaacatt caatggatat cgttcgttca tttgatgagg
     3541 catcggccaa tgcacggcag gcagggaaga gaaaactccg ctggccggtc tcagagatcg
     3601 tggtcgttac cgatgatgac gatgtcatcg cagcgcttga atcactctca tcaatctgca
     3661 aagatcgggc aaatgcacgg gctgtccgcg tagtccgtgg agcatatgac cggatcgggt
     3721 ggaaggccga accggtgatg aaagcccttg gcccggcatt tggaaagaat gccccgatag
     3781 tgcgcgaact gataaggtcc gccgatgccg ctgccctgaa gtcacccctt gacaagggtg
     3841 aaacggtgac ccttgctgga agcgatgggg tgtacccgat tcagccgggc tatgtcgcct
     3901 ttcttgaaga acttcccccc aatctctttc aggcgcatat ggaacgggga acagtctatg
     3961 tggatgtcac gctcacggct gaccttgaag cagaagggta tgcccgcgaa gtgatccggc
     4021 gaattcagga gatgcggcgg caaagggatc tgaatgtcga ggattacatc acggcaggca
     4081 ttatcataga tgatgaacga gtatgctcac ttctgcaggg aagatgggaa gacgtaatcc
     4141 aggaggaggt acgtgcggga tcgctcagca tctccccagg atcctcggtc tccatgaatg
     4201 gctcatttga actcgaaaga gactgggaca tcgaaggtat ccaggcagtg atcggcatat
     4261 caaagttcac ggatcaaacc ggaaagaaac agacggcctt caggtgagga gaagaccggc
     4321 ggttcagaga tacacttgac tgaggtacgg atagttttta ccatcttttt ttcgacaggg
     4381 ttgaaccctt caatctcttt tgcagtcttg taaacgaaga tgccacgccg ttgccatgcc
     4441 ggggtttttg cgagattgag tccccgtgaa aacatcatct catgcagttc tgaagaggaa
     4501 aggcccttta acagctgggc ggcctcatgc gaagcttttc cctctccaac cagggtctga
     4561 tgacaatatg agttgctatg attcctccat gcctccgcct gccggtcgat gagatactgg
     4621 agggcataga gggggcttac ctggatgatc cgggcatcaa acgcaacagg aaatggggat
     4681 gacatctcga tcgtcagggc acttgcagca taggatgcgg ttatcgagtc aatcttctct
     4741 actctgcctg aataaggaac atgtgaaaaa tagaggttta tctcatcaga aaaggtaaaa
     4801 gcgagatccg gggagagtcc actggaggtg ataagccgtt cgctgacacg ggccatagcc
     4861 cgggcaaacc gggagtcgaa aggccgcgtc agtccccact cttctgctac ccggtgaaat
     4921 ccccgcccgt ctagacgcac gaatacaggg ggtacagaaa gcaggttgct gaatatttcg
     4981 cgccttttca tatcatgaat cccggacacc ttttggtagc tttccgcctt tcatctccgt
     5041 tttccattgc tagaaccacg gttcctgata ccagtattcg ataaaattct tcctcccggg
     5101 cacgtgctgt cactctcata cgtgggacca ttcagcagga aagcatccca gaaaaaccgg
     5161 ataggattct ccctgaaggg gaggatccga attctggctg gttatcagat atcttcagcc
     5221 ctgaaagcac ccggcaggtg gcggatgaga acaatgtccc cgatgctcct g
//
LOCUS       DAOOCO010000142         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_14719_length_5270_cov_4.573921, whole genome shotgun sequence.
ACCESSION   DAOOCO010000142 DAOOCO010000000
VERSION     DAOOCO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14719_length_5270_cov_4.573921"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..839)
                     /locus_tag="PK154_08070"
     CDS             complement(222..839)
                     /locus_tag="PK154_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW11052.1"
                     /translation="MTEEIIVIEPGDERAQKIGKAIGSPMANEILQILAGGPKTASDL
                     TDLLNIPMGTLKYHIDNLLEAGLIEIAETRYSVKGREVKVYALRDQLLIVAPKVTSVR
                     SILLKYASLFAIVVLASMAMFAILPLLDMGTTQMTEPASFQIDRSAGGGEGASILAEK
                     ATAPAAAPVLPSSVPMVLAFFLGGCLVLLVLILYEAYRWQKGYSP"
     gene            complement(832..3573)
                     /gene="alaS"
                     /locus_tag="PK154_08075"
     CDS             complement(832..3573)
                     /gene="alaS"
                     /locus_tag="PK154_08075"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPW11053.1"
                     /translation="MLEDEYQLEYFKIQGMTRKVCTRCGAAFWTRNPDQEICGDAPCE
                     TYNFIGNPVFRRHTVDEMREAYLSFFEQNHHTRINRYPVVARWRDDIYLTIASIADFQ
                     PWVTSGVVPPPANPLTISQPCIRLNDFDSVGRSGRHLTLFEMMAHHAFNSDFEEVYWK
                     DRTVELCEQFLSSIGGNLEQVTYKEHPWIGGGNAGPSLEVLIGGLEVATLVFMNLGKQ
                     NTGKPPVDLDGQLYYPMKNWIVDTGYGLERLVWASRGSPTIYDAVFPEMVSRVMESAG
                     LEHFLDNQEYTKILSLNAKFAGVMDISGTNLYQLRKKVATAIDVPIEKLDRMITPIEK
                     VYAVVDHSRCLAYMLGDCIVPSNVREGYLARLVLRRTLRMINDLGIRDNLADLIEQQM
                     RIVGTGSFEQDILIVREIVEREVEKYNATLQRGTKIVQKVARTYKAKRERLPLKEIIT
                     LYDSHGIPPEITRDVAVAEGAVVELPDNFYSLIADLHSESGENQGDDPFARFRDRIGS
                     LPTTRKLFYEQPCSMEFDAMVIDFFEGYVVMDQTLFYPEGGGQPADAGMLIGAESMVR
                     VDDVQKIGDVIIHHISGGVLRRGDRVKGIIDEERRWSLMRHHTATHLLLHAAKEVLGA
                     HIHQSGAQKGVESARLDIRHYKHITIEELRKIEVAANRMVMADQKVDIMHEERTKAEQ
                     RYGFALYQGGVPPGKDIRIVQVAGDVEACAGTHCRGTGDIGMIKIIRVEHIQDGIERL
                     EFAAGMAAVEYVQRLEQIVADAASILSVQQEILPSSVSRFFSEWKEQRKEIERLSQQL
                     VEMEVRNLAPEAIKGVPLVIRQIDLPPRDLTSIASHVADKGGVALLASAKDRAHVVLM
                     SGSQMVDAGEIIGQVCVLLGGKGGGSKTMAQGGGPITDQLGLALKVGRERILAALND"
     gene            complement(3609..3962)
                     /locus_tag="PK154_08080"
     CDS             complement(3609..3962)
                     /locus_tag="PK154_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPW11054.1"
                     /translation="MEIDELFEALMEEKELTPELRDEITRIFGARGKKALQAIDERRI
                     KRYHDFFVVVGTSDEYIVDDDFCTCRDFIFRKGRCWHELAVRIAAAIDSFEEVDLWYQ
                     AAWTTKTPPDKQYLS"
     gene            complement(3953..4660)
                     /locus_tag="PK154_08085"
     CDS             complement(3953..4660)
                     /locus_tag="PK154_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPW11055.1"
                     /translation="MDIRAVIRELEEIAPPDGAEEMDSGRIGLIIAGTREIGRVACAL
                     DATPAVVKASVSSGADMLVVHHTPLWSPVTAVPQALAAFLKPVLAADLNIYVMHTNFD
                     HAKGGINDALAGLLGLTSVEEMSLGVCGDCNLDLPGLARCLGFPLRTWGLVTLPCRIG
                     AVGGSGFDPELIGEAASLGATAYLSAELRHSVARGSPLPLIESTHYALEVPGMRQLAE
                     RMGWTFIDDPPVMETWR"
     gene            4753..>5270
                     /locus_tag="PK154_08090"
     CDS             4753..>5270
                     /locus_tag="PK154_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPW11056.1"
                     /translation="MEKTISLIRERIRDGNARVVTAEEMPAIVAELGEEGALREVDVV
                     TTGTFGAMCSSGAFLNFGHADPPIKMEKIWLNDVEAYGGIAAVDAYIGATQKSATREE
                     SYGGAHVIEDFIAGKTVELRALSTGTDCYPRRTITTELLLENLNQAIMLNPRNGYQRY
                     NAASNSTDRSLRT"
BASE COUNT         1132 a         1526 c         1502 g         1110 t
ORIGIN      
        1 aaaattgacc gtgcccggct ctcatatatc tgtaaaaaaa agtgataccc ctctcaatat
       61 caaagaggaa gatatcttgc ttatgcgcaa tcgcatcgca ggggactcaa cagcggcagg
      121 ggggagcaaa acccaacccc cgggagagtc tgccacgcaa tggaacctca taaataatat
      181 gaaaaaaatg gtgtcggaaa ggaccactct caccaggagt atcacgggga atagcctttc
      241 tgccaccggt atgcttcata caggatgagg accagcagca ccaggcaccc tccaaggaag
      301 aatgccaaga ccattggaac agaggagggt agtaccggtg cagcagcggg tgcagtggct
      361 ttttcggcaa ggattgaggc accctcaccc ccgccagcag accgatcgat ctggaacgaa
      421 gccggctccg tcatttgggt ggtccccatg tcaagcagcg gaaggatggc aaacatagcc
      481 atcgaagcga ggacaacgat cgcaaagagt gatgcgtact tgaggaggat cgaacggacg
      541 ctcgtcacct tcggggcaac gatcagcagc tggtcgcgga gggcatagac cttgacctcc
      601 cgacccttca cgctgtagcg ggtctcggca atctcgatca ggccggcctc gagcaggttg
      661 tcgatatggt atttcagggt ccccatcggg atgttcagga gatccgtcag gtcggatgct
      721 gtctttggac ccccggcaag gatctgcagg atctcgtttg ccatggggct cccaattgcc
      781 tttcctatct tctgtgcccg ttcatccccg ggctcgataa cgatgatctc ttcagtcatt
      841 gagtgctgcc aggatccgct cacgtcccac tttcagggcg agccctaact ggtcggtgat
      901 gggcccacca ccctgggcca tggtcttcga accgccacct ttcccgccga gcagcacgca
      961 gacctgcccg ataatttcgc cggcatcgac catctggctg ccggacatca gcacgacatg
     1021 cgcacggtct tttgcactgg caaggagggc taccccaccc ttgtcagcca catgagaggc
     1081 gatggaggtg aggtccctgg gagggaggtc aatctgccgg atgacgagtg gaaccccctt
     1141 gatcgcttcc ggggcgaggt tccgtacttc catctcgacc agctgctggg agaggcgttc
     1201 gatctctttt ctctgttcct tccactcgga gaagaaccgc gacaccgatg aaggtagaat
     1261 ttcctgttgc acggagagaa ttgaagcagc gtcggctacg atttgctcaa ggcgctgtac
     1321 gtactcaacg gccgccatcc ctgcagcaaa ttccaggcgc tcgatcccgt cctggatatg
     1381 ctccacccgg atgatcttga tcatccctat gtccccggtt ccccggcaat gggtcccggc
     1441 acaagcctcg acatcaccgg cgacctggac gatgcggata tctttgccag gaggcacccc
     1501 tccctggtag agggcaaagc catatcgctg ctcagccttc gtccgttcct catgcatgat
     1561 gtccaccttc tgatcggcca tgaccatccg gttggctgcc acctcgatct tccggagctc
     1621 ttcaatggtg atatgcttgt agtgcctgat atccagccgg gcactctcga cgcctttctg
     1681 ggcgccggac tggtggatgt gggccccgag cacctccttt gcggcatgaa gcaggaggtg
     1741 ggtagcggtg tgatggcgca tcagagacca gcgccgctct tcatcgatga tgcccttgac
     1801 ccggtcaccc ctccggagaa ctcctccgga tatatggtgg atgatcacgt caccgatctt
     1861 ctggacatcg tccacacgga ccatgctctc tgccccgatc agcatgccgg catcggcggg
     1921 ttggccaccc ccctcagggt agaagagggt ctgatccatg acgacatatc cctcaaagaa
     1981 gtcgatgacc atggcatcga actccatgct gcagggctgc tcgtagaaga gcttccgagt
     2041 cgtcgggagg ctcccgatac ggtctctgaa ccgtgcaaag ggatcatcgc cctggttctc
     2101 ccctgactcc gagtggaggt cggcgataag cgagtagaag ttgtcaggca gttcgactac
     2161 tgcaccctca gcaaccgcca cgtctctggt aatttcaggg gggataccgt gcgagtcgta
     2221 cagggtgatg atctccttga gggggaggcg ctcccgcttg gccttatagg tgcgggctac
     2281 cttctggacg atcttggtcc cccgctggag cgtggcattg tatttctcca cctcacgttc
     2341 aacgatttcc cgtacgatga gaatatcctg ttcgaacgat ccggtgccca cgatccgcat
     2401 ctgctgctcg ataaggtcgg caaggttgtc cctgatgccg agatcgttta tcatgcgaag
     2461 ggttctccgg agcaccaggc gggcgaggta tccttccctc acgttggatg gtacgatgca
     2521 gtcgccgagc atgtaggcaa ggcaccggga atgatcgacg acggcatata ccttctcgat
     2581 gggggtgatc atccggtcga gcttctcgat cgggacatcg atggcggtgg cgaccttctt
     2641 cctgagctgg tacaggttgg taccagagat gtccatgacg ccggcaaatt tcgcattgag
     2701 ggagaggatc ttggtgtatt cctggttatc gagaaagtgt tcaagccctg ccgattccat
     2761 caccctgctg accatctcag ggaagactgc gtcgtagatg gtcggggatc cccgggaagc
     2821 ccagacgagc cgttcaagac cgtacccggt atccacgatc cagttcttca tcgggtagta
     2881 gagctgcccg tcgagatcga ccggtggttt cccggtgttc tgttttccca ggttcatgaa
     2941 gacgagggtg gcaacttcca gaccgcctat cagcacctcg aggctcgggc ctgcatttcc
     3001 ccctccgatc cacgggtgct ccttgtaggt tacctgctca aggttccccc cgattgaaga
     3061 gaggaactgc tcgcagagtt cgacggtcct gtccttccag tagacctcct cgaaatccga
     3121 gttgaaggca tggtgtgcca tcatctcgaa gagggtgagg tgcctcccgg aacgtccgac
     3181 cgaatcgaag tcatttaacc tgatgcaggg ttgcgagatg gtgaggggat ttgcgggtgg
     3241 gggtaccaca ccgctggtca cccagggctg gaaatcagca attgaggcga tcgtcaggta
     3301 aatgtcgtcc cgccaccggg caacgacagg ataccggttg atccgggtgt ggtggttctg
     3361 ctcaaagaac gagaggtaag cctccctcat ctcgtcgacc gtgtgtctcc tgaacacagg
     3421 gttgccgatg aaattgtagg tctcgcaggg ggcatcgcca cagatctcct gatccggatt
     3481 ccgggtccag aatgctgcac cgcaccgggt gcataccttg cgggtcatcc cctggatttt
     3541 gaaatattcg agctgatatt cgtcctcgag catggataac cacgttgtac ctcttatttt
     3601 taggtttgtt acgataaata ctgtttgtca ggcggggttt tagtggtcca ggcggcctga
     3661 taccagagat cgacctcttc gaacgagtcg atggcagcag cgatacgcac ggcaagctca
     3721 tgccagcagc ggcctttcct gaagatgaaa tcacggcagg tgcagaaatc gtcatccacg
     3781 atatactcat ccgaagtgcc gaccaccacg aagaaatcgt ggtatctctt aatccgccgc
     3841 tcatcaattg cctggagggc ttttttcccc ctcgccccga atatccgcgt gatctcgtcc
     3901 cgcagttccg gggtgagttc tttctcctcc atcagggctt caaacagctc gtctatctcc
     3961 atgtttccat taccggagga tcatcgataa atgtccaccc cattcgttcc gcgagctggc
     4021 gcataccagg gacttcgagg gcgtaatgcg tcgattcgat cagcgggagc ggggagcccc
     4081 gtgcaactga atgtctgagt tccgcagaga ggtaagctgt ggccccaaga gaggcggcct
     4141 ctccgatgag ctccggatca aacccactcc cgcccaccgc cccgatccgg cagggaaggg
     4201 taaccagccc ccaggttcga agtgggaaac caagacacct ggcaaggccc gggagatcga
     4261 ggttgcagtc accgcagacg ccgaggctca tctcttccac agaggtaagc ccgaggagtc
     4321 cggccagggc atcgttgatc cctccctttg catgatcaaa gttggtatgc atcacgtaga
     4381 tgttcaggtc cgctgccagc accggcttga ggaaagcggc cagcgcctgc gggacagcgg
     4441 ttaccgggga ccaaagcggg gtatgatgca cgacaagcat atcggcacca gaactgaccg
     4501 atgccttgac tacggccggg gtggcatcca aggcacaagc tacccgtccg atctcccgcg
     4561 ttcctgcaat gatgaggccg atcctgccgc tgtccatctc ttcggcacca tcgggaggag
     4621 cgatctcctc gagctcccgg atcaccgccc tgatatccat gggaaggtat cagtgccacg
     4681 gggctaaaaa gtatgtaatt acccttggta ataagcaaca cttataaata ttcataaaaa
     4741 gacatcatgg ctatggaaaa gaccatcagc ctgatcaggg agcggatcag ggacggaaac
     4801 gcacgggtgg tcacggcaga ggagatgccg gcgatcgttg ctgagctcgg tgaagaggga
     4861 gcgctccgtg aagtggatgt ggtcacgacc ggtacattcg gggcgatgtg ctcatccggc
     4921 gcattcctga actttggcca tgcagacccc ccgatcaaga tggagaagat ctggctgaac
     4981 gacgtcgagg cgtatggcgg catagcagct gtcgatgcct atatcggtgc gacgcagaag
     5041 tccgctaccc gcgaggagag ctatggcggt gcccatgtca tcgaggactt catcgcggga
     5101 aagaccgtcg aactcagggc tctctccacc gggaccgatt gttacccgag gagaactatc
     5161 accaccgaac tcctcctcga gaacctgaac caggccatca tgctcaaccc gaggaacggg
     5221 taccagcggt acaatgccgc ttcgaattcc actgacagaa gcctccggac
//
LOCUS       DAOOCO010000143         5156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_15159_length_5156_cov_3.858655, whole genome shotgun sequence.
ACCESSION   DAOOCO010000143 DAOOCO010000000
VERSION     DAOOCO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15159_length_5156_cov_3.858655"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(531..689)
                     /locus_tag="PK154_08095"
     CDS             complement(531..689)
                     /locus_tag="PK154_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HPW11057.1"
                     /translation="MAKKAGGRLISSAGLVNYYESEDKRAIHINPIAVIVVAVAISVF
                     ILVLNLFT"
     gene            738..1781
                     /locus_tag="PK154_08100"
     CDS             738..1781
                     /locus_tag="PK154_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW11058.1"
                     /translation="MPRSECLVEGRALIGERLEEREVAIVVRGGRIHRIEDRAPRPGA
                     PWICPALFNAHTHLGDSVAMDLRAEGSLESLVTPPHGLKHQILEKTPPCELIRAMRAT
                     LEFMQEAGTAGCADFREGGPAGVQALRTAAEGISCVPVIFGREGGEAYADGLGVSSAR
                     DIHGLEQIVRRSREMKRLIAFHAGERDRFDIDSALDYAPDLIVHCTHATSRQLRRCAE
                     EEIPIAVCARSNWTLGVTDSPGKPPLKKMIEAGCRIVLGTDNCMFVQPDLWREMSFIA
                     TVYQIPPALIFRAAVEGSCLTKTPPFITEGSPARFLVIHPGDSNLWLSHDMITTLVKR
                     AGRKNIVTKVFNC"
     gene            1818..2255
                     /locus_tag="PK154_08105"
     CDS             1818..2255
                     /locus_tag="PK154_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW11059.1"
                     /translation="MFRHILVAIDGSKISDNALKAAIEEARVWKAEVHAIYVVETGLF
                     SSLPMDSTWEIMYSMLEKEGKTALDTAKEMGEQHGVKIQTYIRQGHAGNEILKAINEL
                     PVDLVVMGSHGKSEVDRILLGSVSSFVVSNSPKTVMVVRPSKP"
     gene            2252..3109
                     /locus_tag="PK154_08110"
     CDS             2252..3109
                     /locus_tag="PK154_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW11060.1"
                     /translation="MNVSDFMTTEVVTVEIPGNRDDVLKILKRTGISGVPVLKEGKLV
                     GIITRKDLLRKADETQLGLLMTRNPATVSPDTPVEEAARILIEKNVRRLPVVEGEKLV
                     GLLSVADLVHAIAQLRIKDEIKPTYISQTFALWEETPLPLVARIMEISGFDAIPILDA
                     ESKLQGIISERDLIRHSSIEDMVEVSDFSNGTDDDEWTWESIRDMHTISYGISKIQLP
                     DKPVKTAMVSNVISVPLNAEVSECALKMKRGRVDQLPVVNGDKKLVAMLFDRDLIRAM
                     CRAPDNKNL"
     gene            3186..3827
                     /gene="psmB"
                     /locus_tag="PK154_08115"
     CDS             3186..3827
                     /gene="psmB"
                     /locus_tag="PK154_08115"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HPW11061.1"
                     /translation="MSEQYQEPLKGTTTVGLVFEDGVVLATEKRATMGYMIASKKAKK
                     VYQIADRIGMTIAGGVGDAQQIARIMTVECSLYNIRRGRAMSVAAASTLLSNILNNNR
                     YYPFYVQLLVGGVDDSGPGLFSVDAMGGATKEEEIVATGSGSPMAYGVLEDRFTKGMK
                     RKDAISLAVRAVRAAMKRDAGSGEGVHVVVITKDEYFEVPEDQLPKNSAAATA"
     gene            3851..>5156
                     /locus_tag="PK154_08120"
     CDS             3851..>5156
                     /locus_tag="PK154_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HPW11062.1"
                     /translation="MLIEDRLKELKNKIDERVPAGITVSEVEFEGPELVIYTDDPKRF
                     ADEADLIKILARDLRKRIVVRPNVLEDPERAMLEIREVVAENAGITDIFFDADTGEVL
                     IEAEKPGVVIGKNGATLREITKHIGWTPKVVRTPPIESSTVKQVRQFLRSVNEDRKQF
                     LRTIGRRIHRDVTSKDAWVRVTMLGCCREVGRAAFLLSTPESKILIDCGEKPDNSTNN
                     APYLYVPEIHPLSQLDAVVLTHAHLDHCALVPLLYKYGFDGPVYSTPPTRDLSAMLQL
                     DYLDVEKKEDRKIPYSSNDVKSYIKHSITLNYGSVTDIAPDIKLTFHNAGHILGSAIA
                     HFHVGDGLYNVAFTGDFNYSKSRLFNPATNQFPRLEAIFMESTYGGSGDLQPARADAE
                     EKLYETIGNVISRGGKVIIPAFAVGRSQEVMLALEEGIRREKI"
BASE COUNT         1258 a         1366 c         1489 g         1043 t
ORIGIN      
        1 cagtggtctt ccggaactgt cccacctgga acggccatga caacctgtac ttctctcccg
       61 aagaagacat catgaggagg ctgcttacct catgagtgtc aggatctgca ccggaacggc
      121 tcccgaatga agccatcaga atgacgatcc tggactcctg acttttgagg gccctgcggg
      181 aatcctcccg atactgagat ctcgaggagc ccccctgtcc cttgcagatg aggagctgtg
      241 cagacacagg tcccgattga agccatacaa tgagatacct gactttttgg cctgcaggag
      301 ctgtgctgga gcagaacgtg caaggagctc tactggagca gatcgttttg cagaatcacc
      361 ccgcaggaga tcgggagcat ccccccggca tcccaggctg caaagggcgg gatcatggtt
      421 cacccccttt atgccgggaa aagaccggta cggccatctc cttcgaatag gtacaacggt
      481 gttgccgccc gggaatcgga aaaacagagg gaaggggggg atacctgatt tcaggtaaag
      541 aggttcaata caaggatgaa taccgaaatt gcaaccgcta ccacaataac agcaatggga
      601 ttgatatgga tcgcccgttt atcctcgctc tcgtagtagt tgaccagccc ggcagaggag
      661 atcagcctgc ctcctgcttt ttttgccata cgtattaatt tctcattatc cctatttaaa
      721 atcggtgaat ccgggatatg cccaggagcg agtgtctggt ggaagggcgg gcccttatcg
      781 gagagaggct tgaggagcga gaggtagcga ttgtcgtccg cgggggcagg atccaccgca
      841 tcgaagaccg tgcgccccga ccgggtgccc cctggatctg cccggccctt ttcaatgcac
      901 acacgcacct gggagacagt gtggcaatgg acctccgggc agaggggagc ctggagagcc
      961 tggtcacccc tccccacggg ctcaagcacc agatactcga aaaaacaccc ccttgcgagc
     1021 tgatccgggc gatgcgcgca accctggagt tcatgcagga ggcagggaca gccggatgtg
     1081 ccgatttccg ggaaggcggc ccggcaggag tgcaggcact gcgcaccgct gcagagggga
     1141 tctcctgcgt cccggtcatc ttcggccgtg aagggggaga agcatatgcc gatgggctgg
     1201 gggtcagcag tgcaagggat atccacggtc ttgaacagat cgtacgccgg tcgcgtgaaa
     1261 tgaagaggct gatcgccttt catgccggag agagggaccg gtttgacatc gattcggccc
     1321 ttgactatgc ccccgatctc atcgtgcact gcactcacgc gacgagccgc cagcttcgca
     1381 ggtgtgcaga agaggagata cccatcgccg tctgtgcccg gtcgaactgg accctcgggg
     1441 tgaccgattc cccgggcaaa ccgcctttaa aaaagatgat agaggcaggg tgcaggatcg
     1501 ttcttggaac tgacaactgc atgttcgtcc agcccgacct gtggcgcgag atgtccttta
     1561 tcgcgacggt ctaccagatt cctcccgccc tcatattcag ggcagcggtg gaaggatcgt
     1621 gcctcacaaa gactccccct tttatcacgg agggatctcc cgcccggttc ctcgtgatcc
     1681 accccggtga ctccaacctc tggctctccc atgacatgat aacaaccctg gtcaaacggg
     1741 ccggacggaa aaatatcgtc acaaaagttt ttaattgctg aaaagacatt aatagaaaag
     1801 ctcttttgga gagtaccatg ttccgacaca tccttgtggc catagacggg tcaaaaataa
     1861 gcgataatgc attgaaggcc gcgatcgaag aggcacgggt ctggaaggca gaggttcatg
     1921 ccatctacgt tgtggagaca gggctctttt cctccctgcc catggacagt acctgggaga
     1981 ttatgtacag catgcttgag aaggaaggaa agactgcgct ggatacggca aaagagatgg
     2041 gagaacagca tggcgtcaag atccagacct acatccgcca ggggcatgcg ggaaacgaga
     2101 tcctgaaagc aatcaatgaa cttcctgttg accttgtggt gatgggatcc catggaaaga
     2161 gcgaggtaga ccggatactc cttggcagtg tcagttcatt tgtcgtctca aactctccaa
     2221 aaacagttat ggtggtgcga ccatcgaaac catgaacgtc tctgatttca tgaccaccga
     2281 agtggtcact gttgagatac cggggaacag ggatgatgtc ctgaagatac tgaagcgtac
     2341 cggaatttcc ggggtccctg tcctcaagga agggaagctc gtcgggatca tcacgagaaa
     2401 agacctcctg cgcaaggcag acgagaccca gctcggcctc ctgatgacac gcaaccctgc
     2461 gaccgtctcc cctgacaccc ctgtcgagga ggccgcccgc atccttatcg agaagaacgt
     2521 gagaaggctt ccggtggtcg aaggggagaa actcgtcgga ttgctctccg tcgccgatct
     2581 tgtccatgcc atcgcccagt tgcggataaa ggacgagata aagcccacgt atatcagcca
     2641 gacctttgcc ctctgggagg agacacccct cccgcttgtc gcccgtatca tggagatatc
     2701 agggttcgat gcgatcccca tcctcgatgc agagagcaag ctccagggga tcatatcaga
     2761 gcgtgacctg atccggcatt cttccatcga ggacatggta gaggtgagtg atttttcgaa
     2821 cgggaccgat gacgatgaat ggacctggga gagcatccgg gacatgcaca ccatcagcta
     2881 tggtatctca aagatccagc tccccgataa accggtcaag accgcgatgg tcagcaatgt
     2941 catctccgtg cccctgaatg ccgaggtgag cgaatgtgcg ctcaagatga agcgggggag
     3001 ggttgaccag ctcccggtcg tgaacggtga caaaaaactg gttgccatgc tctttgaccg
     3061 tgacctcatc agagcaatgt gccgtgcacc ggacaataaa aacctttaaa ttttcggatg
     3121 acatttaatt gttgaagcgc ttttgtgaga tttttccagc gttttccatg ttttggggtg
     3181 aatatatgag tgagcaatat caggagcccc tgaagggaac aaccactgtc ggacttgtct
     3241 ttgaagacgg tgtcgtgctt gctaccgaga aaagggcaac aatggggtac atgatcgcaa
     3301 gcaagaaagc aaaaaaagtg taccagatcg cagaccgaat cgggatgacc attgcagggg
     3361 gggtgggaga tgcccagcag atcgcccgga tcatgaccgt agagtgcagt ctttacaata
     3421 tcaggagggg gagagcgatg tcggtcgccg ccgcctcaac tctcctctcc aacatcctga
     3481 acaacaaccg gtactacccc ttctacgtcc agctcctcgt gggaggagtt gatgactccg
     3541 gaccaggcct gttctcggtg gatgcgatgg gtggggcaac gaaggaagaa gagatcgttg
     3601 cgacaggatc cggatctccc atggcatacg gggtgctcga ggaccggttt accaagggaa
     3661 tgaaaaggaa agacgcgatc tcgcttgcag tgagagcggt ccgtgctgcc atgaaaaggg
     3721 atgcaggttc gggagaagga gtgcacgtcg tcgtgatcac gaaggacgag tatttcgagg
     3781 tccctgaaga ccagctcccg aaaaacagcg ctgcagcgac ggcataatac ttttttttag
     3841 gactgattgg atgcttattg aagatcggct gaaagaactg aagaataaga tcgatgagag
     3901 agtacctgcg ggtatcactg tctctgaagt ggaatttgaa gggcccgagc tggtcatcta
     3961 taccgatgac cccaagcggt ttgccgatga ggccgacctg atcaagatcc tggcgaggga
     4021 cctgcggaag cgtatcgttg tcaggccgaa tgtgcttgag gatcccgaac gggcaatgct
     4081 tgagatccgt gaggtggtcg cagaaaatgc agggatcacc gatatcttct ttgatgcaga
     4141 taccggtgag gtgctcatcg aggctgagaa accaggtgtc gtcatcggca agaacggtgc
     4201 aacgctccgt gagatcacga aacatatcgg atggacgccc aaggtcgtca ggacaccgcc
     4261 gatcgagagt tcaacagtaa aacaggtcag gcagttcctg cgatcggtga atgaggaccg
     4321 gaaacagttt ctccggacca tcgggcggcg tatccaccgc gatgtcacga gcaaggacgc
     4381 atgggtccgg gtgaccatgc tcggctgctg cagggaggta gggagggcgg cattcctcct
     4441 ctccactccc gagagcaaga tcctgatcga ctgtggagag aaacctgaca acagcaccaa
     4501 caatgccccc tatctctatg tgccggagat tcaccccctt tcacaactgg atgctgtcgt
     4561 gctcactcat gcacacctcg accattgtgc gctggtaccc ctcctgtaca agtacggatt
     4621 tgacggtccc gtgtacagca ccccccccac gcgggatctg tcagcgatgc tccagctgga
     4681 ttacctggac gttgaaaaaa aagaagatcg gaagatccct tacagctcaa acgatgtgaa
     4741 gagttacatc aagcattcca tcaccctcaa ttacgggagc gtgacggata tcgctcctga
     4801 tatcaagctg accttccaca atgcaggcca tatcctcggg tcggcgatcg cccacttcca
     4861 cgtcggagac gggctctata atgtcgcgtt taccggtgac ttcaactaca gcaagagcag
     4921 gctctttaat cctgccacca accagttccc gcggctcgaa gcgatcttca tggagagtac
     4981 ctacgggggc agcggcgatc ttcagccggc ccgtgcagat gccgaggaga agctgtacga
     5041 gacgatcgga aacgtcatct ccaggggtgg aaaagttatc atcccggcgt ttgccgtggg
     5101 gcgatcccag gaggtcatgc ttgcgctgga ggaggggatc aggagagaga agatcc
//
LOCUS       DAOOCO010000144         5115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_15314_length_5115_cov_5.553953, whole genome shotgun sequence.
ACCESSION   DAOOCO010000144 DAOOCO010000000
VERSION     DAOOCO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15314_length_5115_cov_5.553953"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK154_08125"
     CDS             complement(<1..488)
                     /locus_tag="PK154_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11063.1"
                     /translation="MKKNGRKSKICPILLGLGILLMTVSITSAANDVTESIQCPFESI
                     EETKIILDSTPEIIDENFFQIISNIRSITPFTKRSYTLMAGEGISLQIPRTILYSSGQ
                     YTVPTTIVDMQLQTGLQKKNGVIWDLVASKTAERDTTDELSQSGIYYALPGTYRTVGC
                     HKV"
     gene            complement(864..1655)
                     /locus_tag="PK154_08130"
     CDS             complement(864..1655)
                     /locus_tag="PK154_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPW11064.1"
                     /translation="MADLLRTVTSSDKRKNLLVMLDSGPRSWDQIRDALQVTSTGMLP
                     QIKILIDRGLVRKEKDRYCLTDLGQTVAHSLVPLVRVTDVFDTLEGYLSDHDFSVLPA
                     SFRYCIGDIAGIRVTEVETAEVFEPHQTFLNNLANAQCIFGIVPVLYPSHSTFFVNLA
                     REGIEVSIVMTQAIFNRVRDEHQDELNEGLNLPNLHFYLSDEDIRFACVATDRYLWLS
                     LYTKSGIYDPKKELISYEPSARQWGERLVRYYQDRSRNITLDQTP"
     gene            complement(1928..2488)
                     /locus_tag="PK154_08135"
     CDS             complement(1928..2488)
                     /locus_tag="PK154_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HPW11065.1"
                     /translation="MDLLTAEEGACAVRLARSVLEQKIGGTTFPPIPLAPIFNEKRGV
                     FVTLTRDGELRGCIGFPYPTFPLGEAIRDAAISAALQDPRFPPVRATELPCIRVEVTV
                     LTVPQLMTAEPAARPGAVEIGKHGLIARGYGRSGLLLPQVPVEWKWDSREFLDHTCMK
                     AGLPRGCWKEDCVEFFTFEGQIFHEE"
     gene            2523..2863
                     /gene="rnpB"
                     /locus_tag="PK154_08140"
     ncRNA           2523..2863
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK154_08140"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            2937..3503
                     /locus_tag="PK154_08145"
     CDS             2937..3503
                     /locus_tag="PK154_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW11066.1"
                     /translation="MHIPTPDELRARRERLGIRQTELAERAGISQSMVARIEAGSVDP
                     RTSTLRKIVNVLNTAEQSRMTAAHVMHTPVFSVQASEPVTRALEIMNLNNISQIPVIE
                     DGVPVGCISESAIVSVIEEQSAHRAVGLAVKNFMEPGFPTVPPGMDVDTVVRILQNNH
                     AVLVMDAGTVTGVITKHDLISLIVDRAP"
     gene            complement(3668..4336)
                     /gene="tpiA"
                     /locus_tag="PK154_08150"
     CDS             complement(3668..4336)
                     /gene="tpiA"
                     /locus_tag="PK154_08150"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPW11067.1"
                     /translation="MTKAFILINLKTYSEGAGQRAHNIAGAAEQVADESGVLIALAPS
                     YMNIHPVSMHYSLPVYAQHVDGAEPGAHTGAITAEALKMAGAVGSLVNHSERRLTLAD
                     IESSVTALKNASLIPVVCSNNEATSAAAAALSPAFVAIEPPELIGSGVSVSKANPDII
                     KRSVEAVRRVNPDVEILTGAGIQSGECVKIALDLGTSGVLLASSVVKAKAPIAVLRDL
                     VSML"
     gene            4320..4484
                     /locus_tag="PK154_08155"
     CDS             4320..4484
                     /locus_tag="PK154_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11068.1"
                     /translation="MKAFVMDELPDTRVIVPEHNSSPGRPAGPGVYRRNHPEEMPVQS
                     AGQPDQELQA"
     gene            complement(4634..>5115)
                     /locus_tag="PK154_08160"
     CDS             complement(4634..>5115)
                     /locus_tag="PK154_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPW11069.1"
                     /translation="NRLGDSGQVSVLVQGNIPAMITADTLLDLVRERGDHRKRSFEFT
                     AMIYGIKDTTGRIFPVHHDPWGRTHILNAAELCLIDYLPDLARAGVDSWIIDARWRGP
                     SYAAGMVSVYRDAVENMPWMAGDLTSAEHIQALKTRIRAMAQGGITAGHYLRGLSSE"
BASE COUNT         1199 a         1332 c         1378 g         1206 t
ORIGIN      
        1 actttgtggc atccaactgt gcggtatgtt cccggtaagg cataataaat tccggattgt
       61 gaaagctcat ctgttgtatc acgctctgcg gttttggatg cgacaaggtc ccagatgaca
      121 ccattctttt tctgcaatcc tgtttgaagc tgcatgtcaa caatcgttgt gggaactgta
      181 tattgacccg aggagtagag tatagttctg ggtatctgaa ggctgatccc ctcccctgcc
      241 attaatgtat aagacctttt tgtgaagggt gtaattgatc tgatattact aataatttgg
      301 aaaaaatttt catcaattat ttcgggtgta ctatccaaaa ttatttttgt ttcttctatt
      361 gattcaaatg gacattggat cgattcagtt acatcattcg cagcagatgt aatagacacc
      421 gtcataagta gtattcccaa tcctaaaagg attggacata tcttactctt tcgaccattc
      481 tttttcatgg aataatcaac tccttctgta atcaaatgat tgcaatattt ggttactttt
      541 gatggtaata gatacgtctt ggtgatcttt ctgggccatc tatcctacct tttttcctta
      601 aacatttata tataatccat tatatttttt aacgtcattg aaaaatttaa tatcattctt
      661 caacattccg cacggtaaaa tttaaaaaaa tttggtgatt cctttgatat cagggcagta
      721 caattttcag tgacctggta ggattatcca aatatcatgt ccttgttaaa gatacatcgt
      781 attgggttgt gcagtgaccc catcgcacat ccaggctctc ttcgtattgg tcatgtctgg
      841 gtaggaattc tttcgggttc tgctcagggg gtctggtcca gtgttatgtt acgggacctg
      901 tcctggtaat acctgaccag tcgctccccc cattgtcgtg cagagggttc atagctaatg
      961 agttcttttt ttgggtcgta aatgccgctc ttggtataga gagagagcca gagatagcgg
     1021 tcggtagcga cacaagcaaa gcggatatct tcatctgata gataaaagtg taaattcggc
     1081 aggttcaatc cctcgttaag ttcatcctgg tgttcatccc tgacccggtt gaagatggcc
     1141 tgggtcatca cgatggagac ctcaatccct tccctggcga gattgacaaa gaatgtcgaa
     1201 tgagaaggat agagaacagg tactatcccg aagatgcatt gcgcattggc gagattgttg
     1261 aggaatgtct ggtggggctc gaagacttcg gctgtttcga cctcggtgac ccggattccc
     1321 gcgatgtcac cgatacaata tcggaacgaa gcagggagca ccgaaaagtc atggtccgag
     1381 agatacccct ccagggtgtc gaacacatcc gtgacccgga caagcgggac gagggaatga
     1441 gcgaccgttt gcccgaggtc tgtcaggcaa taccgatctt tctccttccg tacaagaccc
     1501 ctgtcaatga ggatctttat ctgcgggagc atgccggtgg aggttacctg gagtgcatcc
     1561 cttatctgat cccatgaccg tggccctgaa tcaagcatca caagaaggtt tttcctcttg
     1621 tctgaggagg tcacggtccg gaggaggtct gccatgctcc tgttctgggc gtcgatgtat
     1681 aaaacttttc ttgatcctgc cagttatact ctcaaaccat acagtttgct ggagtatcgc
     1741 gtctgaaact gcccatggtc ctggcatact gtgcgaggga attcaccaga ctggagctgt
     1801 cgtctcattg gagagaggtt gctatcgaac tcctccacta tcagggagaa ggatttcgcc
     1861 cggtccggta tgatctacac caggattcag ccggacaggc ggttgatatc ccctgctctt
     1921 gaatcattca ctcttcgtga aaaatctgcc cttcaaaagt gaagaactcc acgcagtcct
     1981 ccttccagca tccccggggg agtccggcct tcatgcaggt atgatcaagg aattcccggc
     2041 tgtcccattt ccattcgacc gggacctggg gaaggaggag gccgctccgc ccgtagcccc
     2101 tggcgatgag gccatgcttt ccgatctcaa ctgctcccgg gcgggctgcc ggttctgcag
     2161 tcatcaactg tggtaccgta agaacggtca cttccacccg aatgcagggg agttcagtgg
     2221 cccgaacggg gggaaacctg gggtcctgga gggctgcaga gatggcagca tcccgtatcg
     2281 cttccccaag agggaaggtg ggatagggaa acccgataca gcccctcagt tcaccatccc
     2341 tggtaagagt cacaaagact ccccgcttct cattgaagat cggggcaagg ggaatagggg
     2401 ggaaggtcgt gccgccgatc ttctgctcga gcacagaccg ggcaagccgg acggcacagg
     2461 ctccctcttc tgcggtgagg agatccatca agagtaaata atgaagagtc ccatatttat
     2521 aggagaggga gtgtgcggct gacggtggcc gatacggctg ctgaggaaag tccccccacc
     2581 acctggacac gtagccatac gcaagtatgg gtggcgagag tcacggcaat ggcacagaaa
     2641 cgacacggcc tgctccgagc gatgatacgg ccgagccttc acgggcaaga cgcgaccgga
     2701 aacggcgcaa gataacccgc tgagctgaag ggcaggatgc gatgaaacgg cgaacccctg
     2761 cgggtgcaag ccagaacagg gtacggtggc gtccaggcat tacccgggta tggcgcgtag
     2821 ctgaatgccg caccgaacag aagggggctt actcctccca ctcattcctg gttctgaacc
     2881 ggtttttgat tcgtactgca tagtattaac gtctcatatg ccccagagta atacctatgc
     2941 acatccctac tcccgatgaa ctgagagcac gaagagagcg gctcgggatt cgccagacag
     3001 agctggctga aagggcaggg atcagccagt cgatggtcgc ccggatcgag gccgggagtg
     3061 ttgacccgag gacgagcaca ctcaggaaga ttgtgaatgt cctgaacacc gcagaacaat
     3121 cccgcatgac cgcggcccat gttatgcaca ccccggtctt ttcagtccag gcttctgagc
     3181 ccgttacacg ggcacttgag atcatgaacc tgaacaacat ctcccagatc ccggtcatcg
     3241 aggacggggt cccggtcggc tgcatctccg aatccgctat tgtgagtgtt atcgaggagc
     3301 agagcgcaca ccgggcagtc gggcttgccg taaaaaattt catggagccc gggtttccca
     3361 cggtacctcc ggggatggat gtcgatacgg tggtcaggat cctccagaac aatcatgcgg
     3421 tgctggtcat ggatgccggg accgtgaccg gagttatcac caaacacgat cttatctctt
     3481 tgattgtcga tcgtgcgccc tgatctgctg gagacctaat tttctgagac ccgttacggc
     3541 tgtgtatgca ccaatcggag agcaggggaa gtattgttcg attcttaaaa aataccaggt
     3601 gattcccgtg tttcacctga acgccaaaac aaaaaagccg ggggaatttt tcccgaaaaa
     3661 aaggctctta caacatcgag acgaggtccc gtaatacagc gatcggtgcc ttggccttga
     3721 cgacactgga ggcaaggagg accccgctcg taccgaggtc aagagcgatc ttcacgcatt
     3781 cgccggactg tatccctgca ccggtaagga tctcaacgtc aggattcact ctcctgactg
     3841 cctccacgga tcgtttgatg atgtcaggat ttgccttcga gaccgagacc ccgctcccga
     3901 tcagctcggg cggctcgatg gcgacaaagg ccggtgagag ggccgctgcc gcggcactgg
     3961 tggcctcatt gttcgagcag accacgggaa ttagactggc gtttttcagc gctgtcaccg
     4021 aggattcaat atcggcaagg gtcagccgcc gctcggagtg gttcacgagc gatcccactg
     4081 ccccggccat cttcagcgcc tcagccgtta tcgcccccgt gtgggcgccc ggctcggccc
     4141 cgtcaacatg ctgggcatac accggaaggc tgtaatgcat cgagacgggg tggatattca
     4201 tgtagctcgg cgcgagcgca atcagaaccc cgctctcgtc tgccacctgt tctgcagcac
     4261 cggcgatatt atgggcacgc tgccccgctc cctcggaata ggtcttcagg ttgatcagga
     4321 tgaaggcttt tgtcatggat gaactccctg atacaagagt gatcgtgccg gaacataaca
     4381 gttcacccgg caggccggca ggaccaggag tatacaggcg gaatcatcct gaagagatgc
     4441 ccgttcagtc agcgggccag cccgaccagg aattacaagc atgatcctgt cgatatcggg
     4501 caattcaccc ggcagagaga ccaaaccagg atcattcagg agtgatcatg tctgtgatga
     4561 tcgctcacct ggcttgatac gagacaagaa tgatacaaga ttgattgtgc caggaacgac
     4621 aggaagcacc tgttcactct gaagagaggc cgcgcaggta gtggccggcg gtgatacccc
     4681 cctgtgccat agccctgatc ctggtcttca gagcctggat atgctctgca gaggtgagat
     4741 ctcctgccat ccatggcata ttctccactg catcgcggta gaccgagacc atccctgctg
     4801 cataggacgg tcccctccac cgggcatcga tgatccagct gtccacccct gcacgggcaa
     4861 gatcggggag gtagtcgata agacagagct cggcggcatt caggatatgg gtccttcccc
     4921 agggatcgtg atggacgggg aagatccttc cggtagtgtc cttgatgccg tagatcatcg
     4981 cagtgaactc aaaagacctt ttccgatggt cccctcgctc tcttacaagg tcaagcaggg
     5041 tatcggcggt gatcatcgcg gggatattcc cctggacaag gacgctcact tggcccgagt
     5101 caccgagacg gttac
//
LOCUS       DAOOCO010000145         5047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_15606_length_5047_cov_5.155649, whole genome shotgun sequence.
ACCESSION   DAOOCO010000145 DAOOCO010000000
VERSION     DAOOCO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15606_length_5047_cov_5.155649"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK154_08165"
     CDS             <1..666
                     /locus_tag="PK154_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW11070.1"
                     /translation="CASGMSEAFQKAGKKTNVIVYAGDGGTVDIGIQALSGAFERETD
                     FLYICYDNEAYGNTGMQRSGSTPIGARTTTTPGGKVHAKKDLDAIVAAHNPSYLATAC
                     AAYPQDLFKKVQKALTMRGSKFIHVLAPCPPGWRFSSEMTVEMGKLAVRSGLWILYES
                     EYGKVTINGPSKAAMIKPVPIEDYLSVQGRFKGIDRKAIDALKSQAEQRAKHLQKEAE
                     GIC"
     gene            660..1772
                     /locus_tag="PK154_08170"
     CDS             660..1772
                     /locus_tag="PK154_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPW11071.1"
                     /translation="MLTIATGNKAVAAAVKEVKPDVIAAYPITPQTEIVEQIAEYVTS
                     GELDSRYIPVESEHSAMAACIGASITGARTFTATSSHGLLYMHEMVNWAAGARLPIVM
                     ANVNRAIGPGWNVWAEHTDAFQERDTGWLQVYVSTVQEAYDTTLMAFRIAEHADVILP
                     VMVNLDGFLLSHSMQSLDTLNAGDFVPPLHLPHAIDVLNPAGYGTLTGPDEHFRFRWE
                     IEKSMRRSRKVIAETERDFAERFGRSYGVAEEYRYEDADVVVIAMGTLGKEAEVAVDL
                     LRNEGVNAGSLRIRWLRPFPDLDLSGREVVVIDRDYSFGFGGVIAQSLMARQRVDLYS
                     VIAGLGGQEVTYDDIAGFVRDRRPGTELWFGVNGNV"
     gene            1765..2292
                     /locus_tag="PK154_08175"
     CDS             1765..2292
                     /locus_tag="PK154_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW11072.1"
                     /translation="MYEIRIHSRGGQGGVTAARLLALAAFRDGKYATACPFYGAERRG
                     APVVSYVRIDDRPIRVYSQIRNPDLVVVLDPSVMEVVDVLHGLKRGGKVLMNGTTTEK
                     IHGVPAFAVDLTGIALREKLVIAGSPILNTPVLGALAKMGIISVDSAKKAIQEMFSDE
                     RNVRAAEAAFQELVI"
     gene            2289..2534
                     /locus_tag="PK154_08180"
     CDS             2289..2534
                     /locus_tag="PK154_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW11073.1"
                     /translation="MSTRPAISRPVKGASGKTGSWRVFKPVVDKERCNACGICAMFCP
                     DAAISEDLVVDLDFCKGCGICAHECPKKAISMEREEK"
     gene            complement(2822..3190)
                     /locus_tag="PK154_08185"
     CDS             complement(2822..3190)
                     /locus_tag="PK154_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11074.1"
                     /translation="MTDRFIFTRYRTTCYHCHKEADQIIKAVPYQSQVICENCGATRI
                     FIPRREEVTKEGSFTRIGCYDVWNIVSDASCRNCRTTGPHDLAIGCNHFTARCRNCGF
                     THFYKFDLEYVAKCSIEGKD"
     gene            complement(3331..4992)
                     /gene="thsA"
                     /locus_tag="PK154_08190"
     CDS             complement(3331..4992)
                     /gene="thsA"
                     /locus_tag="PK154_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HPW11075.1"
                     /translation="MAQQLGGQPILILKEGSSRTRGRDAQGMNITAAKAVASAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDDEVGDGTTTAV
                     VIGGELLKRAEDLLEQDVHPTVIAQGYRQASEKAQEILQNIAITIKPTDAAMLKKIAE
                     TAMTGKGAEASKEKLCDLVVKAVMMVADDDGTVDKDNIKVEKKVGGSVDDSEIVEGVL
                     IDKERVHPAMPKKVKNAKILLLNAAVEFKKTEVDAEINITSPDQLQAFLDEEERMIKN
                     IVDKIVASGANVLFCQKGIDDIAQHYLAKAGIFATRRVKKSDMEKLARATGGNLVSSI
                     DAISKDELGYAGLVEEKKVSGEEMIFVVECKNPKAVSLIVRGGTEHVVDEIERAVEDA
                     LRVVGVVFEDKKLVAGGGSPEIELSLRLREYAATVGGRAQLAIEAFANALEIIPRTLA
                     ENAGLDPIDMLVELRAAHEKGKKTVGLNVFDGKAEDMLKSGVVEPLRVKTQAISSAAE
                     AAVMILRIDDVIAASKTAAPQGPPGGMEGMGGMGGMGGMGGMGDY"
BASE COUNT         1101 a         1348 c         1413 g         1185 t
ORIGIN      
        1 tgtgcatccg ggatgagcga ggcgtttcag aaagccggta agaagaccaa cgtgatagtc
       61 tatgccggcg atggtggaac ggttgacatc gggatccagg ctctctccgg tgcattcgag
      121 cgggagaccg atttccttta tatctgctac gataacgaag cctacggcaa tactggtatg
      181 cagcgttcgg gttccacccc gataggtgcc cgcaccacca ccacgccggg ggggaaggtt
      241 catgcaaaga aggatctcga tgccattgtt gccgcccaca atccttccta cctcgcaaca
      301 gcctgtgctg cctaccccca ggacctcttt aaaaaggtgc aaaaagcact cacaatgagg
      361 ggctcaaagt tcatccatgt ccttgcgccc tgcccgcccg gatggcgctt ctcctcggag
      421 atgacggttg agatggggaa actcgcggtg agatccggcc tgtggatcct ctatgagagt
      481 gagtatggga aagtgaccat caacgggcca tctaaagctg cgatgatcaa accggttccc
      541 atcgaggact atctttcagt ccagggaagg ttcaagggga tcgacaggaa ggctatcgat
      601 gctttgaagt ctcaggcaga acagcgggca aagcaccttc agaaggaggc cgaagggata
      661 tgttgacgat tgccaccggg aacaaggcgg tcgcggctgc ggtgaaagag gtgaagcccg
      721 atgtcatcgc cgcatacccg atcactcctc agaccgagat tgtcgaacag atcgcggagt
      781 atgtgacctc gggtgaactg gacagtcgct acatcccggt cgagagcgaa cattcagcaa
      841 tggcagcctg catcggcgca agcatcacgg gtgcccggac cttcactgcc acaagttcgc
      901 acggtctcct ctacatgcac gagatggtca actgggccgc aggggcacgc ctccccatcg
      961 tgatggccaa cgtgaaccgg gcaatcgggc cgggctggaa cgtctgggct gagcataccg
     1021 atgccttcca ggaacgggat accggctggc tccaggtcta cgtcagcacc gtccaggagg
     1081 catacgatac caccctcatg gcgttcagga tcgcggaaca cgccgacgtg atccttcccg
     1141 tgatggtcaa cctggacgga ttcctcttgt cgcattccat gcagtcgctc gacaccctga
     1201 atgcggggga cttcgttcca ccgctccatc tgccgcatgc gatcgatgta ctgaatcctg
     1261 ccggttacgg gactctcacc ggaccagatg agcacttcag gttccggtgg gagatcgaga
     1321 agtcaatgag aagatcccgg aaagtcattg ctgagacaga gagggacttt gccgaacggt
     1381 tcggaaggag ctatggggtt gccgaagagt accgttatga agatgcagat gttgtggtca
     1441 ttgcgatggg aaccctcggc aaggaggcag aagtggcggt tgatctcctc aggaatgaag
     1501 gggtgaacgc aggatccctc cgtatcaggt ggctgcgccc cttcccagat ctcgacctct
     1561 cggggcggga agtcgtggtc attgaccgag actactcgtt tggattcgga ggagttattg
     1621 cccagtcgct gatggccagg caacgggtcg acctgtatag tgtgattgct ggtctcggag
     1681 gccaggaggt cacctatgat gacatagcag ggtttgtgcg ggacaggaga cccggcaccg
     1741 agctctggtt cggggtgaac ggcaatgtat gagattcgta tccactcccg tggcgggcag
     1801 ggtggtgtga cggctgcccg gctccttgct cttgcggcct tccgggatgg gaaatatgca
     1861 acggcgtgcc cattctacgg ggcagaacgg cgcggtgctc cggtcgtctc ctatgtcagg
     1921 atcgatgacc ggccgatacg ggtctacagc cagataagga atccggatct ggttgttgtg
     1981 ctggatccga gcgtgatgga agtcgtggat gtccttcatg ggctgaaaag gggtggtaag
     2041 gtgctgatga acggcactac caccgagaag atacacgggg tcccggcctt tgcggtcgat
     2101 cttaccggca tagcactccg ggagaagctg gtaattgccg gaagcccgat actgaacacc
     2161 ccggttctcg gggcacttgc aaaaatgggt attatatcgg ttgattcggc taaaaaggct
     2221 atccaggaga tgttttccga tgaacgcaat gtcagggcag cagaagcagc cttccaggag
     2281 ctggtgatat gagtacccgt cctgcaatca gcagaccggt caagggggct tcaggaaaga
     2341 ccggatcctg gcgggtattc aaacctgttg ttgacaagga gcgatgcaat gcatgcggca
     2401 tctgtgccat gttctgcccc gatgcggcaa tcagcgagga ccttgtggtt gatctcgatt
     2461 tctgcaaggg atgtggtatc tgtgcacatg aatgccccaa gaaagcgatt tcgatggaac
     2521 gggaagagaa ataatcgcgg ttgtaatgtt tctatctctc ttttttatca gacaatctgt
     2581 ctacccgcgc tgattaaaag cggtttctat aaccttgaag taggtattgg ctgaatgacc
     2641 ggcccgactt atccagacag ggaaatagta gggaacgaat catgaacgtt ccttgataca
     2701 gagttgtggt aacgtgatct cgttcaaagc ttgggagatt tgatcaggag ggcacttgat
     2761 tttcactggt ttaccatagt caaatggtgc tcatcatctg aactgaacga caagaagaag
     2821 atcagtcttt cccttcaatg gaacatttag ccacatactc caggtcgaac ttgtagaaat
     2881 gggtgaatcc gcagttcctg caccgtgccg tgaagtggtt gcagccgata gccaggtcgt
     2941 ggggaccggt ggtccggcaa ttccggcagg atgcgtcgga cacgatgttc cagacatcat
     3001 aacacccgat cctggtaaaa cttccctcct ttgtaacctc ctcccgccgc gggataaaga
     3061 tccgggtggc accacagttt tcacagatca cctgggactg gtaagggacc gcctttataa
     3121 tctggtctgc ctccttgtgg caatggtagc aggtcgtccg gtacctggtg aaaatgaacc
     3181 ggtccgtcat gcatgatcat ttccatggag gctataagaa agtagtcccc agtaaaattg
     3241 cgacttttaa gagcaccggg tgtcggaaat tcacaaatta acgttcgaga aaatagagat
     3301 ttttcagggg aataggtgag attgaaggga ttaatagtct cccatgccac ccatgccgcc
     3361 catgccaccc atgccgccca tgccttccat gcctcccggg ggtccctggg gggctgctgt
     3421 cttggatgca gcgatgacat cgtcaatgcg gaggatcatc actgccgctt ctgcggcact
     3481 tgagatggcc tgcgtcttga cacggagcgg ttcaacgaca cctgacttga gcatatcctc
     3541 ggcttttccg tcgaacacgt tcaagccaac ggtcttcttg cccttctcgt gagctgcgcg
     3601 aagctccacg agcatatcaa tcggatcaag acctgcattc tcagcgaggg tccgtgggat
     3661 gatctcgagc gcgtttgcaa atgcctcgat ggcaagctgt gcacgacctc cgaccgttgc
     3721 ggcatattca cggagacgga gcgagagttc gatctccggg gatccgccac cagcgaccag
     3781 cttcttgtcc tcaaagacga ctccgaccac acggagtgca tcttcgaccg cacgctcaat
     3841 ctcatctacg acatgctcgg tcccgccacg tacaatgagg gagacagcct ttgggttctt
     3901 gcattcgaca acaaagatca tctcctcgcc ggagactttc ttctcttcaa caagtccggc
     3961 atatcctagc tcgtctttgg agatggcatc gatgcttgag acaaggttgc cacccgtcgc
     4021 acgggcaagt ttttccatgt cactcttctt gacacgccgg gttgcaaaga tgccggcttt
     4081 tgcgaggtag tgctgggcga tatcatcaat ccctttctgg cagaagagga cgtttgcccc
     4141 agacgcgacg atcttatcca caatattctt gatcatccgc tcctcttcat caaggaaggc
     4201 ctggagctga tcagggcttg tgatgttgat ctcagcgtcg acctcggttt tcttgaactc
     4261 gactgcggca ttcaggagga gaatttttgc gttcttcacc tttttcggca ttgccgggtg
     4321 gacccgttcc ttgtcgatga gtactccctc aacgatctca gagtcgtcaa cggatccacc
     4381 gactttcttc tccaccttga tgttatcctt gtcgaccgtc ccatcgtcat cggctaccat
     4441 catgacagcc tttaccacga ggtcacagag cttctccttt gatgcctcgg cacccttacc
     4501 agtcattgcg gtctcggcga tcttcttcag catcgctgca tcagttggct tgatggtgat
     4561 ggcgatgttc tgcaggatct cctgggcttt ttcagaagcc tggcggtatc cctgggcgat
     4621 tactgtcggg tgaacgtcct gttcgagcag atcttctgcc cgcttcagca gttctccgcc
     4681 gatgaccacg gccgtggtgg tgccgtcccc gacttcatcg tcctgggtct ttgcaatctc
     4741 caccatcatc ttggctgccg ggtgctcaat gtccatctct ttcaggatgg taaccccgtc
     4801 attggtaatc acgacatcgc cgatcgtgtc gacaagcatc ttatccatgc ccttggggcc
     4861 gagcgtggtc cgtactgcgc ttgcaacggc ctttgcggcg gtaatattca taccctgtgc
     4921 atcgcgtcca cgggtacgtg aacttccttc tttcagaata aggatcggtt gtcctccaag
     4981 ctgctgtgcc atagatattc accacttgta gcgataaaat tggtttgtgg ttctatataa
     5041 aatttat
//
LOCUS       DAOOCO010000146         4957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_15996_length_4957_cov_3.826193, whole genome shotgun sequence.
ACCESSION   DAOOCO010000146 DAOOCO010000000
VERSION     DAOOCO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15996_length_4957_cov_3.826193"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PK154_08195"
     CDS             <1..1315
                     /locus_tag="PK154_08195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HPW11076.1"
                     /translation="PIIDNEGKFLGLVSEHGLAKAYVTPRRDASLSIGPIALGTLARI
                     LDARICVEGKREICGRVEIVIDALHVALSRLTPEDVAVVGDNEPAQLALISSGIAALV
                     IAEGAPAGSRTLDAAAAKGVSILASPLDAFSVGRMVHLSLPAGSVLATDVPVLRLDDT
                     LTYAKKAVTDSKYRTACVVDKEGRLLGTISRNTLLGEIQKQVILLDHNEYGQAVDGIE
                     AAEILEVIDHHRLGAITTLKPVRFQNEPVGSTSTIIAMKFMEAGKDPSRPVAGILLAG
                     ILSDTLVLKMSTTTPADEKAVAYLAGICGLDPVGFGTELIQKGMDLDHTPLPALLSRD
                     TKRYSLFGREVVISQVMMASDEFLQGRDDEIRAVLERLRKEDAADLYLVLFTNVIGNH
                     SHLFAAGDLVVLSSLGLDAQPVHLPGVMSRKKDFLPEFGQKLRDL"
     gene            complement(1371..1655)
                     /locus_tag="PK154_08200"
     CDS             complement(1371..1655)
                     /locus_tag="PK154_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11077.1"
                     /translation="MIILRCRACNGTGEVTNEQYRICQALKSHAAKRYFHYPMEEVAG
                     DENEQQKHDACNGVPEKVDCPICEGAGEVSFEEDDWELRIVADEDDHLEE"
     gene            complement(1827..2066)
                     /locus_tag="PK154_08205"
     CDS             complement(1827..2066)
                     /locus_tag="PK154_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HPW11078.1"
                     /translation="MGFKDEVLTQIAALLTAAFGLVAALAWNGAIQALFKQVFGTADS
                     LNAQLIYAVIVTIIAVIVTIWIARAVAHAKGGEKK"
     gene            2388..2693
                     /locus_tag="PK154_08210"
     CDS             2388..2693
                     /locus_tag="PK154_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HPW11079.1"
                     /translation="MSMMKEFMDFLYEYKVIGLAIAFIIGVAATALIKSLVDNILMPI
                     ITFFIPGGAWQTASITIGSIVITWGKFLADLIYFIIVAFVVFIIAKKVLKEDKVEKK"
     gene            2932..3003
                     /locus_tag="PK154_08215"
     tRNA            2932..3003
                     /locus_tag="PK154_08215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2965..2967,aa:Arg,seq:gcg)
     gene            3213..4520
                     /locus_tag="PK154_08220"
     CDS             3213..4520
                     /locus_tag="PK154_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW11080.1"
                     /translation="MDRLRIMTGILIACIGIGLSITPCLAGDRYLSGGPDLFVSLGST
                     SELIPGATEELPVTIGNKGTITLETYNYLSMQPMYLPTTAKSTEIELLPGDAPVVVRS
                     NPQIIGEVQAGQIVPVTFIVDVPQAAPAGNYTMLARISYSYVPRIEQQGTADMTYYFK
                     ENETVLPLPIEIRRMVILAVEKVQSNDLSAGGEGVIDFTIRNSGQDTGIGTSLFLVPE
                     GASPFIPFSNSVYIGEFPPGSTAQPRFKVAVSDDADPGLSYPLSLYAAYKDFEGAAAT
                     SPSVTAGVSFQDKVSFERVSAPSVVHPGTKSTVSVTYRNTGNSTVYNAKARISVINPF
                     SSDDDTAYLGDLSPGESATALFSVKTDGSAMIKTYSADSEVRYTDEGATDYTSDNIPV
                     LIDVQADSATGMAGAILAILVIAGGAFLWYRRKKKTESQGNAP"
     gene            4517..>4957
                     /locus_tag="PK154_08225"
     CDS             4517..>4957
                     /locus_tag="PK154_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11081.1"
                     /translation="MRYLALQRCTRLVAVVLLCITMTASPCIALGYTYLDTGLEPVLS
                     AQVVGDPSYYPGDTFAMTVVLTNEDQDTSVQVAPLLAPDIYDPRMALGLVVSPLGSDA
                     PVSVKTPPVLVGDISPGEKVPLTVQGTVDEEALPGTRTILLNLTY"
BASE COUNT         1116 a         1414 c         1301 g         1126 t
ORIGIN      
        1 cccgatcatt gacaacgaag ggaagttcct cgggctcgtc agcgagcacg ggctggcaaa
       61 ggcatatgtg actcccaggc gcgatgcatc gctctccatc ggccccatcg cccttgggac
      121 gcttgcacgg atcctggacg cccgcatctg tgtcgagggg aaacgcgaga tctgcgggag
      181 ggtggagatc gtgatcgatg ccctccacgt tgccctctcc cggctgacac cagaggatgt
      241 tgccgtggtg ggagacaacg agcctgccca gctcgccctc atctcctcgg ggatagcagc
      301 tctcgtgatt gcagaaggag cccctgccgg gagccgcacg ctcgacgctg ccgctgcgaa
      361 gggggtatca atcctggcaa gcccgctcga tgcattctcg gtcgggagga tggtgcacct
      421 ctcgctgccg gcaggctctg tccttgccac cgatgtcccg gtcctccggc tcgatgacac
      481 cctcacctat gcaaagaagg cggtcactga ttcaaagtat aggactgcct gcgtggtaga
      541 caaggaggga aggctcctgg gcacgatctc gcggaatacc ctccttggcg agatacagaa
      601 acaggtgatc ctcctcgacc acaacgagta tgggcaggcg gtggacggga tcgaggcagc
      661 cgagatcctc gaagtgatcg atcaccaccg gctcggggcg atcacgacct tgaaaccggt
      721 caggttccag aatgagcccg tcggttccac ctccaccatc atcgcgatga agttcatgga
      781 ggccggaaag gacccgtccc gcccggtcgc cgggatcctc cttgccggga tcctctcgga
      841 taccctggtg ctgaagatgt ccacgactac cccagcagat gaaaaggcgg tggcgtacct
      901 tgcgggaata tgcggccttg atccggtcgg gttcgggaca gagctgatcc agaaggggat
      961 ggatcttgac catacccccc tccccgctct cctctcccgg gacacgaagc ggtattcgct
     1021 gttcgggagg gaggttgtca tatcgcaggt gatgatggca tccgatgaat tcctccaggg
     1081 ccgcgacgat gagatccggg ccgttctcga acggctcaga aaggaggatg cagccgatct
     1141 ctacctcgtg ctgttcacca atgtcatcgg gaaccacagc cacctctttg ccgccgggga
     1201 ccttgttgtc ctctcttcgc tcggcctcga tgcccagccg gtacatcttc ccggggtcat
     1261 gtcgcggaaa aaggatttcc tcccggagtt cgggcagaag ctacgggatc tttaacgaag
     1321 ctggtattgg aaaggtacca atccatacat ccactgcata aaaaaacggc ctattcctcg
     1381 agatggtcat cctcatcggc aacgattctc agttcccagt catcctcctc aaaggatacc
     1441 tcacctgccc cctcgcagat ggggcagtcc actttctccg ggacaccatt gcaggcatca
     1501 tgtttctgct gctcgttctc gtctcctgcc acctcttcca tgggataatg gaagtaccgc
     1561 tttgcggcat gtgacttgag tgcctggcag atcctgtact gctcattggt gacctcgcct
     1621 gtcccgttgc aggcgcggca gcggaggatg atcatgtcct gacgtgaata tttgtcaata
     1681 ctggcatata agtggatcag aaatcagaca tatcatgccg ggaagaaggt tcaatactct
     1741 gatacccgca tcagatccat tctgaaggtg tatgtgggca gagagtgaga ttcagagtat
     1801 ggagaatccc aaaaaaatga aaaaggttac ttcttctctc cgccctttgc atgggctaca
     1861 gcccgtgcaa tccagatggt aacaatcacc gcaatgatgg tgactatgac tgcatagatc
     1921 aattgtgcgt taaggctgtc agccgtaccg aacacctgtt tgaacaatgc ctggattgct
     1981 ccattccatg ccagtgcagc gaccagccca aaggcggcgg tcaacagggc ggcaatctgg
     2041 gttaatactt catctttaaa tcccattcaa cttcaccaag tataccataa tattgggtta
     2101 tttatataac tatcattgaa ttgactggca ttttacattt cctgaaagat atctcgtttg
     2161 atgagatgag gaaaaggtct ctggctgtag aaacctgatc atcatcaata attatgccac
     2221 gcctacagta acatggtctg aatctcgttc agagagaaag acagaattta ttgtgaaaag
     2281 accatattta gcctgaaatg tgcttatttt ttcattcatt gaccgattcc tttaaattga
     2341 aacactatca acacttttat taaacaattt gtgaggtgta gaactaaatg agtatgatga
     2401 aagagttcat ggatttcctg tacgaataca aggtcatcgg cctggccatc gcattcatta
     2461 tcggtgtcgc tgcaacggca ttgatcaagt cactggtgga caatatcctg atgccgatca
     2521 tcacgttctt tatccctggg ggtgcatggc agaccgcatc cattaccata gggtcgatcg
     2581 tgatcacctg gggcaagttc cttgctgacc tgatctactt catcatcgtg gccttcgtgg
     2641 tatttatcat cgccaagaag gtgctgaagg aagacaaggt agagaagaaa taatcctttt
     2701 ttccaatgaa tatcacttcg tgattttcat aatgtagccc gcatcctgaa atcggggctc
     2761 acaggctgtt tatgtgaaaa taactccagc attttcttcc cggatttttg cgtcccgtaa
     2821 atgaatcatt cttaggggga ccctcctccg gtccggtcct ccggtcgaag aaacgcacag
     2881 ttatttatga aatgaaatga tatgattatg gtcagatctc catctgatcg tgtcccggta
     2941 gggtagtgga catcctagaa gcctgcggag cttttgaccc gggttcaaat cccggccggg
     3001 gcgtaactca cttttttcaa acttcgatct tagcgggata aggaagctct cccctctttc
     3061 gagcaacccc ggcctgatca tgtacgcaac tgccacagga agcgggctgt tccctccgat
     3121 ctcgctcctt tttgcccatc acgatatagg tagattaata aaatccgacc cgtaatacca
     3181 tcacatcgga ttttggtcgg ggaatggtct tgatggatcg cttaagaatc atgacgggga
     3241 ttttaatcgc atgtatcggg atcggactat cgataacccc ctgtctggca ggggaccgct
     3301 acctctcggg tgggcccgat ctttttgtct ccctcggatc gacctctgaa ctgatacccg
     3361 gggcgaccga agagctcccg gtgaccatcg ggaacaaggg caccatcacg ctggaaacct
     3421 ataattacct ctcgatgcag cccatgtacc tccccaccac cgcaaagtcc actgaaatcg
     3481 agctcctccc cggagatgcc ccggtggtgg tgcgatccaa ccctcagatc ataggggagg
     3541 ttcaagccgg acagatagtc ccggtaacct tcattgtcga cgtaccacag gccgccccgg
     3601 cgggtaatta tacaatgctt gcgagaatca gctacagcta tgtgccccgg atcgagcagc
     3661 agggtaccgc tgatatgacg tactatttca aggaaaatga gaccgtactt ccattgccga
     3721 tagaaatcag gcggatggtg attctcgccg tcgaaaaggt ccagagtaac gacctctctg
     3781 caggagggga gggggtcatc gatttcacca tcaggaactc cggtcaggac acagggatcg
     3841 gcacgtccct cttccttgtc cccgaagggg cgagtccgtt cattccgttc agcaacagtg
     3901 tctatatcgg ggagttccct ccgggtagca ccgcccagcc gaggttcaag gtcgcggttt
     3961 cggacgatgc ggacccgggg ctttcttatc ccctgtccct ctatgcggca tacaaagact
     4021 tcgagggcgc tgctgcgacc tcgccatcgg tcactgccgg ggtatcgttc caggacaagg
     4081 tttcgttcga acgggtgagc gctccatcgg tcgtccatcc cggcacgaaa agcaccgtga
     4141 gcgttaccta caggaatacc ggaaactcaa cggtctacaa tgcgaaggcc cgtatcagcg
     4201 tcatcaaccc gttttcaagc gacgacgaca ccgcctatct cggtgacctc agccctggag
     4261 agtccgctac cgccctgttc agcgtgaaaa ccgatggcag tgccatgata aagacgtact
     4321 cggcagactc cgaggtgcgg tacaccgacg agggcgcaac tgattacacc tccgacaata
     4381 tcccggtgct gatagacgtc caggccgatt cggcaaccgg gatggccggt gcgatccttg
     4441 cgatactcgt catcgcgggg ggagcgttcc tctggtaccg ccggaaaaag aagacagagt
     4501 cccaaggcaa tgcgccatga gatatctcgc attgcagcgt tgcacccgcc tcgtggcggt
     4561 ggtcctcctc tgcattacca tgacagcctc cccctgcatc gctctcgggt acacgtatct
     4621 cgacacaggt ctcgagccgg ttctctcggc acaagtcgtg ggtgatccca gttattaccc
     4681 tggcgacacc ttcgcaatga ccgtcgttct gacgaacgag gaccaggaca catccgtcca
     4741 ggtcgccccg ctcctcgccc ccgatatcta cgacccccgc atggccctcg ggcttgtggt
     4801 gagccccctg gggtccgatg caccggtcag cgtaaaaact ccgcctgtcc tggtcgggga
     4861 tatcagccca ggggagaagg tccccctcac cgtccagggg accgttgacg aggaagctct
     4921 cccgggcacc cgcaccatcc tcctcaatct gacgtac
//
LOCUS       DAOOCO010000147         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_16447_length_4857_cov_5.329654, whole genome shotgun sequence.
ACCESSION   DAOOCO010000147 DAOOCO010000000
VERSION     DAOOCO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16447_length_4857_cov_5.329654"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PK154_08230"
     CDS             complement(<1..1286)
                     /locus_tag="PK154_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPW11082.1"
                     /translation="MHALQIVEFCPDPYLDGDTDEYIILSGTGSLDGIVVSDGEGGFR
                     FPDGSLIEGEITIARNSRAYQVTHGCDPDYEWYDYSASVPDVVRSGTFQLANTGDQLQ
                     LYERGNLVQEVTWPGDLSTRQGQVHYSDDDFWDQRVLMLGQSRFTSQEFVSVDGTAFA
                     APDCSLTVYEETVRSARAEILANVYEFSSPVMAQSLIDAGRRGVAVTVLLEGGPVGGI
                     SDEEKWAVAELSKANIPVLLMTSADTIHSPYRFDHAKYLIIDHSQVLVTSENFKSNGF
                     PEGGLSGNRGWGIVLEDEGVAQYFLGIYSHDTSGGWCIPAAFSSGEAEFPTLAKYKTE
                     FSPFRFEDARVIPVVSPDTSYLIRDLITGAKVSLDIEQAYITNQTNGRINPFLDEAIR
                     AARRGVQVRILLDSYYYNTEGPADNDEMVRFINSVG"
     gene            1390..1974
                     /locus_tag="PK154_08235"
     CDS             1390..1974
                     /locus_tag="PK154_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HPW11083.1"
                     /translation="MNGPIVVKIGGSLMDAVDPLIRLFKKRQLAVLIVPGGGRFARQV
                     RETGISGDAAHWMAIAAMEQMGWFIASRGVNPVTSLKVPRRVEVLLPYTLLRNTDPLP
                     HSWDVTSDTISAWIASSLSLPLILLKSIDGIRSGGIHQNLIAEEVPTEDVDPCFLRYV
                     IAHRVNTIVVNGRKTRRLGNLLAGRKVLCSTIGF"
     gene            2025..2183
                     /locus_tag="PK154_08240"
     CDS             2025..2183
                     /locus_tag="PK154_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HPW11084.1"
                     /translation="MIRKCTSCNAPLAEEGAAEFGCPKCGYEIRRCVRCREQSIPYRC
                     PKCGFGGP"
     gene            2187..2444
                     /locus_tag="PK154_08245"
     CDS             2187..2444
                     /locus_tag="PK154_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HPW11085.1"
                     /translation="MGNVALILRVMPESPEVDLDLLAEKIKENVPGLQDIREEPIGFG
                     LKALKIAVVVGDDAGESDTVESDINAIEGVERAEIIELTLT"
     gene            complement(2477..3433)
                     /locus_tag="PK154_08250"
     CDS             complement(2477..3433)
                     /locus_tag="PK154_08250"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPW11086.1"
                     /translation="MAKVTIIGATGNVGTFAAHTIAEIPYVSEILLVGRNGRENLLCG
                     IAQDMKDAFAACGNYTELNWTTDISDTAGSDIVICAAGAARQAGQDRLDLALGNARIV
                     ASTSRLISEHAPDATLLMVTNPVDVMTSVALRYSGLEPSRVFGLGTHLDSMRLKSLIA
                     SFFKVHVSEVHTRIIGEHGESMVPLWSATTIGGIQISNLPAFSGLPVAEMVATVKTSG
                     EAIIRNKGSTVFGPGEAIATLVQTVLGNENRILTVSAYVKSEVHDIGDVCIGVPARIN
                     REGVFPITIKIDQNEIVQFQNSVEKIRGITSELMRLLEKEEL"
     gene            complement(3448..3759)
                     /gene="ffs"
                     /locus_tag="PK154_08255"
     ncRNA           complement(3448..3759)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK154_08255"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(3776..>4857)
                     /locus_tag="PK154_08260"
     CDS             complement(3776..>4857)
                     /locus_tag="PK154_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11087.1"
                     /translation="CYGDPQGISFPKADDPLMKCPVCGDECIREAGEIINMIPSVFAR
                     CKDCRVKLLDKRLPPPREGYQEVCACGKRFLDDVFWHLYSLFRREGIFTGKESLRDVG
                     SPLLHPGYPIASPPFLPERSLVLLSPYPDTAVARVIIDEIPEIRGVVKSGKFVPGITD
                     TSLRKNPRTYELLAGCDVRANVFPTKMGPVVIFQQHSLIHVEFPRAHNPKICSVEDRI
                     TKLKPEWFVDACCGAGTLGLTGARWGIPHVLLNDTWYAAAFWSACNTWVNRKFFRIDD
                     VTIHANYADMSKHPVGRKPVLIAETEGVQQIQIFQADLHSLHQVLPKEPVLAVIDLFD
                     KSDRAAMGLMIRDWTRHVNGEAFIP"
BASE COUNT         1199 a         1302 c         1191 g         1165 t
ORIGIN      
        1 tctccgacgc tgttgataaa acggaccatt tcatcgttgt cagccggccc ctcagtattg
       61 taataatatg aatccagaag gatccgaacc tgcactcccc tgcgtgccgc acggattgct
      121 tcatcgagga aaggattgat cctaccattg gtctggttcg tgatatatgc ctgttcaatg
      181 tcaagggata cctttgcccc ggtgatcagg tctctgatga ggtaacttgt atccggggac
      241 actacgggaa taacccttgc atcttcgaac ctgaacggtg agaactcggt cttatatttg
      301 gctaatgtgg gaaactctgc ttcaccgctg ctgaatgcag caggaataca ccagcctccc
      361 gaagtatcat gagaatagat acccaggaag tactgggcga ctccttcatc ctcaagtacg
      421 atgccccatc cacggtttcc tgaaagtccc ccttctggaa aaccattgga tttgaagttc
      481 tcacttgtca ccagtacctg gctgtggtca atgatcaggt actttgcatg gtcaaaccgg
      541 tagggcgaat gaatagtatc tgccgatgtc atcagcagta ccgggatatt tgctttactc
      601 agttctgcaa ccgcccattt ctcttcatca ctgattcctc cgaccggtcc cccctcgagg
      661 aggacagtaa ccgcaacacc cctcctgcct gcatcaatca gggactgggc catgacagga
      721 ctggaaaatt cgtagacatt agccagaatc tccgccctgg cagatcggac tgtttcttca
      781 tataccgtga gagaacagtc gggtgcggca aatgcagtac catccaccga gacgaattcc
      841 tgactcgtga aacgggactg gccaagcatg agcacccgct ggtcccagaa atcatcatcg
      901 gaataatgca cctggccctg gcgtgtcgac aggtcccccg gccaggttac ttcctgaaca
      961 agatttcccc tttcatacaa ctggagctgg tcgccggtat tagccagctg gaacgtaccg
     1021 ctgcggacta catcaggcac actggcagaa taatcatacc attcataatc cggatcacat
     1081 ccatgggtca cctgatacgc gcggctgttc cgggcgatcg tgatctctcc ttctatcaga
     1141 gaaccatccg gaaaccggaa tccgccttct ccatctgata ccacgatccc gtcaaggctc
     1201 cctgtcccgg aaaggataat gtattcatcc gtatcgccat cgagatacgg atcagggcag
     1261 aattccacga tctgtaaggc atgcacggga atgcacagga tgaaaagtaa gaaaaaaaac
     1321 aacggcccgc gcatagcatt ggtgtttgaa gccttaaatt attaatctgg tgaaatttat
     1381 cctcttttca tgaatggtcc cattgtggtg aagattgggg gaagtctcat ggatgctgtg
     1441 gatcctctca tacgcttgtt taaaaagcgt caactggctg ttctgatcgt accaggggga
     1501 ggacggtttg ccagacaggt acgggaaaca ggaatttcag gtgatgcagc ccactggatg
     1561 gccattgctg caatggaaca gatgggctgg tttatcgcct cgaggggggt gaacccggtc
     1621 acttccctca aagttccccg cagggtggaa gtgctcctcc cctataccct ccttcgaaac
     1681 accgatcctc tccctcactc gtgggacgtc acttctgaca ccatatcagc atggatagca
     1741 tccagtcttt ctcttcccct gatactgctc aagtcaatcg atggaatccg atccggggga
     1801 atccaccaga atctcattgc ggaagaagtt ccgacagaag atgtggatcc ctgcttcctg
     1861 cgctacgtaa ttgctcacag ggtaaatacc attgtggtga acgggcgcaa aacccggcgc
     1921 ctcggaaatc tcctggctgg ccgaaaagtc ctgtgttcaa ccattggatt ttaaaccctt
     1981 cagggaagat aatataggac tgataatcag gagtgaaaca atacatgatc cggaaatgta
     2041 cttcatgcaa tgcccctctt gcagaagagg gtgcggctga attcgggtgc ccgaaatgcg
     2101 ggtatgaaat aaggcgctgt gtgcgatgca gggaacagag tattccctat cgctgcccga
     2161 aatgtggatt cggggggcca taagcgatgg ggaacgtagc actcattctc cgcgtcatgc
     2221 cggaatcccc tgaggttgat ctcgatcttc tcgcagaaaa gataaaagag aacgtgccag
     2281 gtcttcagga tatacgagaa gagcccattg gatttggatt aaaagcactg aaaattgccg
     2341 ttgttgttgg tgatgatgca ggtgagagcg ataccgtaga atcagatatc aacgcgattg
     2401 agggagtcga gagggcagag atcatcgaac tgacacttac ctgattgggg taagtggcat
     2461 ccctgctgat aagacgttac aactcttctt tttcaagaag tctcatcaat tcactggtta
     2521 ttccccgaat tttttccaca gaattctgga actgcacaat ttcgttctgg tcgatcttga
     2581 ttgtgatcgg gaacaccccc tcgcggttta tccgggccgg aactccaata catacatccc
     2641 ctatgtcgtg aacttcgctc ttcacatacg ctgaaacggt caggatcctg ttctcatttc
     2701 cgagcacagt ctgcacgaga gtagcaattg cctctccagg gccgaaaacg gttgaacctt
     2761 tattcctgat aatggcttca ccactcgtct tcaccgtcgc gaccatttcg gcgaccggga
     2821 gcccggagaa tgccggcagg ttgctgatct gtatgcctcc gatagtggta gctgaccaca
     2881 gggggaccat gctctcgcca tgttctccga ttatccgggt atgtacctcg ctgacgtgaa
     2941 ctttgaaaaa tgatgcaatg agcgatttga gcctcataga atcgagatgc gtgccgagac
     3001 cgaaaacacg ggatggctcc agacctgaat atcggagtgc gacggacgtc atcacatcga
     3061 ccggatttgt gaccatgaga agtgttgcat cgggagcatg ctcactgata aggcgtgaag
     3121 tggaagcaac aatccgggca ttcccaaggg ccagatcaag acgatcctgc ccggcctgcc
     3181 tggcagctcc tgcggcacat atcacaatat ccgaaccggc agtatcagat atatccgtcg
     3241 tccagtttaa ttcggtataa ttcccacagg cggcaaaagc gtctttcata tcctgtgcga
     3301 taccacagag cagattttct ctcccgtttc gaccaacaag aaggatctca ctcacatacg
     3361 gtatctcagc tatggtatgc gcggcaaatg ttcctacatt tcctgttgcc ccgataatgg
     3421 tgacttttgc catgataata acaaacagtg gggacacgtt tttggttgac cgcacgtgtc
     3481 agcgttcgtt taagaccccc tcctccgccc cgcaacccca tgggcgccgg atgtctacgg
     3541 taagtctgct cccttccggg cctggaccgt tctccgcagc agcaggtcgc cactccctcc
     3601 ggagttttga tccctgtcca ccgacctcat gggacgaggt ttcaacgtca ggaatcccat
     3661 agctcccgaa gcccgcctcg gccttcctca aacttcgccc cctgcatacc gacggtttca
     3721 ggtgacaggt gacgccgaac cacccggact agtccccgta tatagtatct gaatatcagg
     3781 ggataaaggc ctcgccgttt acgtgtcttg tccagtccct gatcatcagc cccatagcag
     3841 cccggtccga tttgtcaaaa agatcaatca cggcaagcac cggttctttt ggaaggacct
     3901 gatgaagaga gtggagatcc gcctggaata tctgtatttg ttgcacccct tcagtctcag
     3961 cgatcaaaac cggttttcgt ccgaccgggt gttttgacat atccgcataa ttagcatgaa
     4021 tggtgacatc atcaatcctg aagaattttc tgttcaccca cgtattacag gcagaccaga
     4081 acgcagcagc ataccaggta tcattcagga ggacatgagg tatcccccac cttgcaccgg
     4141 ttaaaccgag cgttcctgct ccacagcatg catcaacaaa ccattctggc ttcagcttcg
     4201 ttatccgatc ctcaactgaa catattttgg gattatgcgc tcggggaaac tcaacgtgga
     4261 tcagagaatg ttgctggaag ataacaacag gccccatctt tgtcggaaac acatttgccc
     4321 tcacgtcaca accggcaaga agttcatacg tgcggggatt ttttctgagc gaggtatcag
     4381 taatcccagg gacaaatttc ccggatttga ccaccccgcg aatctccgga atctcatcaa
     4441 ttatcacccg tgcaactgct gtatcagggt agggcgagag gagaacaaga gaacgttcgg
     4501 gtaaaaacgg ggggcttgct atcgggtatc cggggtggag aaggggggat cctacgtcgc
     4561 ggagggattc ttttccggtg aatatacctt cccttcgaaa gagtgagtac aggtgccaga
     4621 atacatcatc gagaaaacgc tttccacagg cgcatacctc ctgatatccc tcccttggag
     4681 gggggagcct tttgtcaagc agtttcaccc ggcagtcttt gcaccgcgcg aataccgaag
     4741 ggatcatgtt gatgatctcc cctgcctccc tgatacactc gtcaccacag acagggcatt
     4801 tcataagggg atcatctgct ttcgggaaag atatcccttg aggatctcca taacacc
//
LOCUS       DAOOCO010000148         4772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_16859_length_4772_cov_5.174263, whole genome shotgun sequence.
ACCESSION   DAOOCO010000148 DAOOCO010000000
VERSION     DAOOCO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16859_length_4772_cov_5.174263"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK154_08265"
     CDS             <1..272
                     /locus_tag="PK154_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11088.1"
                     /translation="TGVMIHYYINTTDPATTYIAQTRLSQMTTPDGATVMMDDDTDLY
                     GPGPFEHTSFASYQVGGANILELKVVIGNPDDSIVIGGIEIFVER"
     gene            complement(548..979)
                     /locus_tag="PK154_08270"
     CDS             complement(548..979)
                     /locus_tag="PK154_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11089.1"
                     /translation="MMNTGTGACRRTLWFRKCGFSPFLTDPGLACTPEGGEWQARFLE
                     CSILSSLKITASRMGDFDPRKHTAEERGIDAKGNYVRGLKMSETRFKNMVTGHSPAEQ
                     QSMRQQVEEAEAKGLRTYQIKHAKGYYNIENGIVIGSAKGK"
     gene            1069..2277
                     /locus_tag="PK154_08275"
     CDS             1069..2277
                     /locus_tag="PK154_08275"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPW11090.1"
                     /translation="MAGCRILIVIALLAMATLVLPSLALVSSSSGIPVLAGEEAIPQG
                     EGSDTLETGAPDRDGTKSAGFPAPNGFPPSVSDCSITTNQNTPVEITLSARDPDGDTL
                     HWFIEAGPLHGTLGPIRGDRVVYTPWPDYAGSDSFRYKVHDGFWDSDTAMVTITIVRG
                     CQSGDPHLFYGTVTIDGRPAPESAIVRATGEGVIQEIAGNPMTTRADGTYGSANRTLD
                     TLAVRGCIPDGTPIMFSVNGLPAEAGDPANNSTWQPAYPFVAGGLTRLDLRVTTPPPP
                     PDYVYITAIGISLSSPENGITKTMRLEKNPELEVTVSAGMFFIDFYAIGGHAFFGQPV
                     LGRDATIGIYENGIPLSVEEHLWFGSSRVTYDYLANETRTFDILVFVNEEPEISTARH
                     ITITTLPSKS"
     gene            complement(2504..2881)
                     /locus_tag="PK154_08280"
     CDS             complement(2504..2881)
                     /locus_tag="PK154_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPW11091.1"
                     /translation="MLEIFKCGNCGKIVTISHEGAGQLVCCGRPMVKQAENSVDASNE
                     KHVPVVEKQGDGILVKVGSVPHPMEPAHYIAWIEVMAGPYLYVKGLKPGEKPEAFFPV
                     SSPDVKVREYCNLHGLWTNKPHH"
     gene            3118..4725
                     /locus_tag="PK154_08285"
     CDS             3118..4725
                     /locus_tag="PK154_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW11092.1"
                     /translation="MKSPSQPQTDQGTTARTSLLAPAEIEHLIEERAAPLRDELEELR
                     RENKKMQVALRESENHLKLTLDAAAAGMWISGRDGSWMATPQLNRIFGLSPDITFHRE
                     QDLMDYIHPEDLQQLIDAWMGSLRDGTRLDHEYRIIWPDGSIHWLAAKGRVCSESGIP
                     RFVGITYDITERKRMEEALRQRLETERALINSSAEIAVLLDIDGIILNINDNYATMLG
                     KRYEELLGVRIWDFIPHEHVSRRKSWFDQAIRSKRTVKFEENRDGKWHDIIINPVLSA
                     DGTVIGVAVSAYDLTERKLAEQQLREYSESLKRSNEDLELFVGIATHDLQEPVRGIVI
                     FSQLLLNQCREGRWQDPGKYLKVIEQSGLQMSSLVNDLREYSRVRTHAKQMEPVDTTT
                     ILSLALNNLHHVIEENHATVTYDPLPVVLGDGTQVAQVFQNLIDNAIKFSREEKTPEI
                     HLSATLSDGMWEFAFRDNGIGIPQKYFSKIFMLFERLHDRDVYPGTGLGLALCKRIIE
                     RHGGRMWVDSEVGVGSTFFFTLPALPS"
BASE COUNT         1094 a         1318 c         1295 g         1065 t
ORIGIN      
        1 tgaccggggt gatgatccat tattacatca acacgactga ccccgcgacg acgtacatcg
       61 ctcagacgcg tctgagccag atgacaaccc ctgatggtgc aacggtcatg atggacgatg
      121 atacggacct gtacgggcct gggccgttcg aacacaccag ttttgcatct taccaggtcg
      181 ggggtgcaaa tatcctcgag ctgaaagtcg tgatcggcaa cccggatgat tcgattgtga
      241 ttggcggaat cgagatcttt gtcgagagat gatatcctcc tccatttccc cttttttaaa
      301 agcgaagagc accaccaaac gttacgatga agatcccgga tgccagccgg acacaggtat
      361 gccgggtgat cccggggaga acttcctgac aaccctctga caggcatacg gacaaaaagg
      421 gaaaagagcg cacccgaaca atagttcctt cgtgtatttg agacacgatc atccaaaata
      481 aaaatggcga agccatttta atagggaaaa aactggtccg gcctgtcaag gccggaggtg
      541 agagcgctca ctttcctttc gcgcttccga tcacaatccc attctcgatg ttgtagtatc
      601 ccttggcatg ctttatctga taggtacgaa gtccctttgc ttcggcttcc tcgacctgct
      661 gtctcatgct ctgctgttcg gcaggggagt ggccggtgac catgttcttg aaccgtgttt
      721 cagacatctt gagaccgcga acatagttgc ctttggcatc gatgccccgt tcttctgcag
      781 tatgcttcct tggatcaaag tctcccatac gtgaagcggt tatcttcaga gaagataata
      841 ttgagcattc gagaaatcgg gcctgccatt ctcccccttc aggtgtgcag gccagccctg
      901 gatcggtgag aaagggtgag aagccgcatt tccggaacca aagggtccgg cggcatgcac
      961 cggtgccggt gttcatcatg ggagcggggt tctcaaggaa gggcaggata accgttatca
     1021 tctgaatatt ttttatagca gaacgctata cgttccccca tcactaccat ggccgggtgc
     1081 cggatactca tcgtgatcgc attgcttgca atggcaacgc tggtcctccc ctcgctggct
     1141 ttggtatcct cctcttccgg gattcctgtt ctggcagggg aggaagccat accccagggt
     1201 gaagggtccg atacgctaga gaccggtgct cctgatcgtg acgggacaaa gtcagcaggc
     1261 ttccctgccc ccaatggatt ccccccctct gtgtctgact gttccataac gaccaaccag
     1321 aacactcccg tggaaattac cctttctgcc agggatcctg atggggatac gctgcactgg
     1381 ttcatcgagg caggaccgct ccatggtacg cttggaccta tcaggggaga ccgggtggta
     1441 tacactccct ggccggacta cgcaggatct gattccttcc gttacaaggt tcatgacggg
     1501 ttctgggatt cagacactgc catggttacc atcaccatcg tcaggggctg tcaatcaggc
     1561 gatccccacc tgttctacgg cacggtgacc attgatggca gaccagcgcc ggagtcagcg
     1621 atagtgcgtg ccacaggaga aggggtgatc caggagatcg cggggaatcc catgaccacc
     1681 cgtgccgacg ggacatacgg gagcgccaac aggaccctgg ataccctggc agtcaggggg
     1741 tgcatccccg atggtactcc gatcatgttc tcggtcaacg gtcttccagc cgaggcaggg
     1801 gatcccgcaa ataacagcac atggcagcct gcatatccgt ttgttgccgg agggttgacc
     1861 cgcctcgatc tcagggtaac aaccccgccc cctccccctg actatgtcta tatcactgcc
     1921 attggcatct ccctctccag ccctgaaaat ggcataacaa agaccatgag gctggaaaag
     1981 aatcccgaac tggaggtcac cgtttcagcc ggaatgttct ttattgactt ttatgccatt
     2041 ggggggcatg cattctttgg ccagccggtg cttgggaggg atgctaccat tgggatctat
     2101 gaaaacggca tcccgctctc agtggaggag catctctggt ttggatcgag cagggtgacc
     2161 tacgattacc tggcaaatga gacacggacg tttgacatcc ttgtcttcgt gaatgaggag
     2221 ccggagattt ccaccgcacg ccatatcacc ataacgacat taccttcgaa gagctgaaca
     2281 gggacgtttc ggacctggct gatattcatg cctggaatac gtatcccgtg tacccgttcc
     2341 tttaccagtt ggatacacca tcgatccttc tgtcctcggc cagaaagggt gactgggggg
     2401 aaattgccct gttgttcgtc acgagggatc atggtttcca gcaaatagct ccgctcttca
     2461 tgacagccag agacagcggc gtgcggtcag gttgtttttg gctttaatgg tgaggcttgt
     2521 tcgtccagag cccgtggagg ttgcagtact ccctgacttt gacatcagga gaggagaccg
     2581 ggaagaacgc ttcaggtttc tcccctggtt ttaagccttt cacgtacagg taggggcctg
     2641 ccatgacctc gatccacgcg atatagtggg ctggttccat agggtggggg actgatccga
     2701 cctttacaag gatcccgtca ccctgcttct caacgaccgg gacatgcttc tcgttgcttg
     2761 cgtcgacaga attctcagcc tgtttgacca tcggcctccc gcagcagacc agctggccgg
     2821 caccttcatg ggagatggtc acgatctttc cacagtttcc gcatttgaaa atttcaagca
     2881 tgctcttttc actcctctgc tgtgatcctt gcctgaaagc gtcataaaca ttcctgacag
     2941 gcagggcgca ggggaagcac cataacgggc gttcccgtga cggagttccc tgggagatgg
     3001 gcatcctctg aacacatccg cagggactct gctttcgtcc ccctctccct gggggatatc
     3061 attataatct atcctataaa cctgttttac tgtctttttc acaggaacga aagatggatg
     3121 aaaagcccgt cacaacccca aactgatcag ggaaccactg cccggacatc ccttctggca
     3181 ccggcagaga tcgagcacct gatcgaggaa cgggctgccc cgctccggga tgaactcgag
     3241 gaactgagga gggagaacaa aaaaatgcag gtggcgctcc gggagagcga aaaccacctg
     3301 aagctcaccc tcgatgccgc ggcagcgggg atgtggatct ccgggcggga cgggagctgg
     3361 atggccactc cgcagctcaa caggatcttc ggactctccc ctgacattac cttccaccgg
     3421 gagcaggatc tcatggacta tatccatccc gaggacctcc agcagctgat tgatgcatgg
     3481 atgggatccc ttcgcgatgg cacgcggctc gatcacgagt accgcatcat ctggccagac
     3541 gggagtatcc actggctggc cgcaaaaggg agggtctgtt ctgagagcgg catcccccgg
     3601 tttgtcggta tcacctacga catcactgag agaaaaagga tggaagaagc cctcaggcag
     3661 agacttgaaa ccgaacgggc gctgatcaac tcctctgccg agattgcggt tcttctcgat
     3721 attgatggaa tcatcctgaa tatcaacgac aattatgcca cgatgcttgg gaaacggtat
     3781 gaagaactcc tcggagtacg tatctgggac ttcataccac acgagcacgt ctcccgtcga
     3841 aaatcctggt ttgatcaggc catccggtca aaaaggacgg taaaatttga agagaaccgt
     3901 gatggaaaat ggcatgatat tatcatcaat cctgtccttt ccgctgatgg aactgtcatc
     3961 ggtgttgcgg tgagcgccta cgatcttacc gagcggaaac tcgcagagca gcagctcaga
     4021 gaatactcgg agagccttaa gcggagcaac gaggacttag agctcttcgt cgggattgcc
     4081 acccatgacc tccaggaacc ggtcaggggg atcgtcatct tctcacagct cctcctcaac
     4141 cagtgcaggg aaggccggtg gcaggacccc gggaagtacc tgaaagtaat agaacaatcc
     4201 ggactccaga tgagcagcct ggtcaacgat cttcgcgagt attcccgggt acggacgcat
     4261 gcaaaacaga tggagccggt tgatacgacg acaatccttt cacttgcgct gaacaatctc
     4321 catcatgtca tcgaggagaa ccacgcaacc gtcacctacg atccgctccc ggtggtcctt
     4381 ggcgacggga cccaggttgc ccaggtcttc cagaacctta tcgataatgc catcaagttc
     4441 agcagggagg agaaaacccc ggagatccac ctctcagcga cgctatctga cgggatgtgg
     4501 gagttcgcgt tcagggacaa cgggattggc attccgcaga aatacttcag caagatattc
     4561 atgctcttcg aacgactcca tgaccgggat gtctatccag ggaccggcct tgggcttgcc
     4621 ctctgtaagc ggatcatcga gcggcatggc gggcggatgt gggtggactc ggaggtcggg
     4681 gtgggatcga ccttcttttt cacgctcccc gcactgccat cgtaaggatc aggaaagagg
     4741 tgcttccaga cacctgttcc cggccatcgt at
//
LOCUS       DAOOCO010000149         4706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_17155_length_4706_cov_4.255859, whole genome shotgun sequence.
ACCESSION   DAOOCO010000149 DAOOCO010000000
VERSION     DAOOCO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17155_length_4706_cov_4.255859"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1040)
                     /locus_tag="PK154_08290"
     CDS             complement(234..1040)
                     /locus_tag="PK154_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW11093.1"
                     /translation="MSPLLAIAAIVLALATAFIAAMAGVGGGFLYVPILTLIFGLAPA
                     EAVGTSLVVIIFTTLAASVSYLRQGRIFFRSTLCLIVPGMTGAIIGAYATVFVPGTLI
                     SVLFSLIVGFLSIKLLFPSFPLVWPVTAGPSCDEVCNDCFSLSVKHRIYYLHYLAWGL
                     MSGLASGLVGIGGGVINVPALVTAGMPVHFATATSTLVVLFTSMTGGGIHALLGHVET
                     TYVVLFSTGALIGGYTGAYFAPRAPERTLRRAIGLLFAGIAVAMGVGSLL"
     gene            complement(1132..1914)
                     /locus_tag="PK154_08295"
     CDS             complement(1132..1914)
                     /locus_tag="PK154_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW11094.1"
                     /translation="MNNTGIDLHKPGEDAAIIVSEATGLAADEGRYQEALTLFDKVPE
                     TGFSRPEVLIKRALTLDRIGLFSEAIRCCEKAICRAPLFSDAWFARGFTRYYLFRASL
                     AIDCLDMALSLDPAHTEARFIRGNCTYKAHDPANAVAVYEKALILSPTYPKALYNRGV
                     CLADMERFEESLASYNACIRINPGVSVVHANRGVTLDSLERYQEALEAFEEALRLDPY
                     DITALNNSCLLLSRLRRDNEAIEYHARFRRLMDLKDFDRRVW"
     gene            complement(2240..2575)
                     /locus_tag="PK154_08300"
     CDS             complement(2240..2575)
                     /locus_tag="PK154_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HPW11095.1"
                     /translation="MDLVFYTDAIKILALFFSLVGAVLIGYGGVAAAIKVVLREVFRR
                     PYSYNLIRREFTSKIVFGLEFFIASDVLTTLIAPTQEEIILLAVVVVIRTILGYFLAR
                     ETVEYQLEP"
     gene            2870..3283
                     /locus_tag="PK154_08305"
     CDS             2870..3283
                     /locus_tag="PK154_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPW11096.1"
                     /translation="MIEVTEEDFNPGEYIEAARTRQTGGIVTFTGIVRDDDITSMDLE
                     AYKEVALEELTLIRDRAMALFQVNSIDIVHRTGRLQVGDHIVMIVVGSPHRKSAFQAC
                     EYIIDKIKESVPIWKKEHTEGGTRWVPGEHHPRTA"
     gene            3292..3756
                     /locus_tag="PK154_08310"
     CDS             3292..3756
                     /locus_tag="PK154_08310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW11097.1"
                     /translation="MDSHIRLFREGDYSGICLLEIQEKGATYPSSVFIRQASVLYPST
                     FLVAELNGILAGYCIGAVCEGGSSEAWILRLRVAPPCRRRQIGSDLVTTLLGIFLQRD
                     VADVYLSVAPENYAARNLYHKVGFRGISLQKGYFGDGEDRIIMAAQLNHSQE"
     gene            complement(3850..>4706)
                     /locus_tag="PK154_08315"
     CDS             complement(3850..>4706)
                     /locus_tag="PK154_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW11098.1"
                     /translation="HVVRAFLSGFQPYLTIVNVPPGGTITVDASLLPLSRIGALQVKS
                     NPGGADIYIDGFYSGSTATTVGNLAAGEHFLTLRLAGYQDWLGTVDIPENSVAFIDAR
                     LEVASAPTTGDILVSSNPPGAAVFNDEVYVGNTMPDDALDLTGLLPGFHTIRLELQNY
                     QDFTVQVEVESGQIRIVDADMVLATNPSQSGSLLVNSGPQGANVFLDNACIGITPLSV
                     SSVTAGNHTLLIRLAGYNDYSTMFTIAPGEAVQIQAALGPAATPINIGILTLSAAAVA
                     MIFFLRKK"
BASE COUNT         1236 a         1160 c         1224 g         1086 t
ORIGIN      
        1 ctcctcggaa cccggaatgc cgttccagac atcattgcac cctctttcac tggaagatga
       61 tatccctgcc ggatcaaaaa aacctgcagt gctgccggat tcctgacagg ttattttgag
      121 gtgcattccg gatcctgccc acaaacgtga accatcgcta tgatcggaaa tagcgccctt
      181 cattggatag ttggcaggat tctcccttaa tcaaatagta cataatatcc ttcctacagg
      241 agactaccaa cccccattgc tactgcaatt cctgcaaaaa ggagacctat tgctctcctg
      301 agtgtccgct ccggagctct cggggcaaag tatgccccgg tatacccccc gataagagct
      361 ccggtggaga acaaaactac gtaggtggtc tcaacatggc caagtagggc atggatccca
      421 cctcctgtca tcgaagtaaa gaggacgacg agggtggagg tcgcggtggc aaaatggacc
      481 ggcatcccgg cggttaccag cgcaggtaca ttgatcaccc cccctccgat tccaaccagg
      541 ccgctcgcaa ggccggacat cagtccccat gcgagatagt ggaggtaata gatcctgtgc
      601 ttcacgctta aggagaagca gtcattgcag acctcgtcgc aggaaggccc ggcagtaacc
      661 ggccagacaa ggggaaagga ggggaagagc agttttatgg acaggaaacc gactatcagg
      721 gagaagagga cgcttatgag agttcctggc acgaaaacgg tagcatatgc tccaatgatg
      781 gcaccggtca ttcccgggac tatcaggcag agcgtactgc ggaagaagat ccgtccctgt
      841 cgcaggtagg atactgaggc agcaagagtg gtaaagatga taacaacgag gcttgttccg
      901 acagcctctg cgggtgcaag cccgaagatc agagtgagga ttgggacata gagaaaacca
      961 ccgcccactc cggccatcgc tgcaataaag gcagtagcaa gggcaagcac gatagcagca
     1021 atggcaagga ggggggacat gatgaattca cttctgagag gatttttggt cagagaccag
     1081 cgtgaaggga gatgatttct ctggtttgat tggtattcct ttcatccttt atcaccagac
     1141 ccgccgatca aaatccttca gatccattaa acgacgaaac cttgcatgat actcgattgc
     1201 ctcgttatcc ctgcgaagcc tcgaaagcag gagacagcta ttattcaggg cagtgatatc
     1261 atagggatcg agtctgaggg cttcttcaaa tgcttcaagc gcttcctgat accgctcaag
     1321 actgtcaagg gttactccgc ggttggcatg cacaacagag accccgggat taatcctgat
     1381 acatgcattg tacgatgcca atgattcttc aaatctctcc atgtcagcaa ggcagacacc
     1441 ccggttgtac agagctttcg gataggtggg agagaggatg agggcttttt cgtacacagc
     1501 aaccgcattc gccggatcat gtgccttgta ggtacaattc ccccggatga accgtgcttc
     1561 cgtgtgtgcc ggatccaaag acaaagccat atcgaggcaa tcaatcgcaa gtgatgccct
     1621 gaaaagataa taccgcgtga acccgcgcgc aaaccaggca tcggaaaata ggggagcacg
     1681 gcatatcgcc ttttcacaac acctgatagc ttctgagaag agaccaatcc tgtcaagcgt
     1741 cagggctctt tttatgagta cttcagggcg ggaaaagccg gtttcaggta ccttatcaaa
     1801 gagcgtgagt gcctcctgat acctgccttc atcagcagca agacctgtcg cttctgaaac
     1861 aatgatagcc gcgtcctccc ctggtttgtg aagatcaatc ccggtattgt tcaatggatg
     1921 tttcccagtg tggactttgc cggatcaccg gaaatattta tttgtcttgc gtttcgtcat
     1981 agggttgttt ttcatttcca gggaagagag atgcaggtac tgccattctc tgtgtatcct
     2041 accgaagatt tcaaggagat atggttaagt gtcatcggga ggcatcagga aaaaaggaga
     2101 ccatgcttca tgaaggttgc caaaatcgcc attctccccc tttcgaaaaa ttgcctgccc
     2161 atactccatc agaaaccagg accatcaaat gttatttgta ttccagcaat ttttagataa
     2221 aaaaaaattc agccgcgtat catggttcga gctggtattc caccgtctcc cgagcgagga
     2281 agtatccgag tatggtccgg ataaccacca ccaccgccag aaggatgatc tcttcctgcg
     2341 tgggagcaat aagcgtagtg aggacatctg aggcgatgaa aaattcaagc ccgaatacaa
     2401 tcttgctggt aaattcgcgc ctgatgagat tataactgta gggacgcctg aatacttctc
     2461 tcagaaccac tttgatggcg gcagcaacgc ctccgtaccc gatcagcacg gccccgacca
     2521 gggagaagaa taacgcgaga atttttatcg catcggtata aaagacaagg tccatccggg
     2581 ttctccctat attccctctc atttcctctc atataaaacc cgtccaaatc ctttgatcaa
     2641 gccatcggtg gaatttcaat ttcagcctgg actggggaag agctggagag cgctctcatg
     2701 agttagacaa tcccatttcc tggatggatc tttcgtcaat aggagagatc actatgtggg
     2761 atggctggat ccaggtatca ttcccaaaaa tcatgatggt atctccaggt aaatcgttat
     2821 gggcaaggtg tgaaataata ggcatgagat taagaaagga tagacaggaa tgatagaagt
     2881 aaccgaggaa gattttaatc cgggagagta tatcgaggcc gcccggaccc gtcagactgg
     2941 agggatagtc accttcaccg gcattgtcag ggatgatgat atcacatcaa tggatcttga
     3001 agcatacaag gaggtagccc tggaagagct gaccctcata cgggaccggg caatggctct
     3061 gtttcaagtc aactcgatcg atatcgtcca tcgcaccgga agactccagg tgggcgatca
     3121 tattgtgatg atagtggtcg gatcccccca ccggaaatct gcgttccagg catgcgagta
     3181 catcattgac aaaatcaagg agagtgtccc catctggaaa aaggagcata cggagggagg
     3241 tacccgatgg gttccgggtg agcaccatcc tcgaactgcc tgaccttcct tatggattct
     3301 catatcaggc tgttccggga gggggattat tccgggatct gcctccttga gatacaggaa
     3361 aagggtgcga cgtacccctc ttccgtcttc atccggcagg catcagtatt gtatccttcg
     3421 acattcctgg tcgcagaact gaacggaatc ctggcaggat actgtattgg tgcggtctgc
     3481 gaaggaggat cttcagaagc atggatcctt cgtctcaggg tagccccacc ctgtcgccgg
     3541 agacaaatag gttcggacct tgtcacgact ctgcttggca tctttttaca gagggatgtt
     3601 gcagatgtgt atctctctgt ggcaccagag aattatgctg cacggaatct ttatcacaaa
     3661 gtgggattca gggggatctc tctccagaaa ggatattttg gagatggtga ggatcgcatc
     3721 atcatggcag ctcagctcaa ccatagtcag gaatgatctc acaccagaag agttcttaga
     3781 ggtgatccgg tacagcgaga agatactcag gtcgttctta tgccattttt ttgcagttcc
     3841 caaaaaaggt tattttttcc ggaggaagaa tatcattgcc actgcagcag cggagagcgt
     3901 gagaataccg atgtttattg gtgtcgccgc aggtccaagt gctgcctgga tctgaaccgc
     3961 ttctcctgga gctatcgtaa acatggtaga atagtcattg tatccagcaa gccggatcag
     4021 gagggtatgg tttcccgcag tcacagaaga aactgacagg ggagtgatac ctatacatgc
     4081 attatccaga aagacattcg caccctgtgg acctgaattt accagaaggc tcccactctg
     4141 tgaagggttt gttgccagga ccatgtcagc gtccactatc cggatctgcc cggattccac
     4201 ctcaacctga acggtaaaat cctgatagtt ctgcagctcc agacggatgg tatggaatcc
     4261 cggaaggaga cctgtcagat cgagggcatc atcaggcatg gtattcccca catatacctc
     4321 atcattgaag acagctgcac ccgggggatt tgacgagaca aggatgtcgc cggttgttgg
     4381 agcggaagca acctcgagcc gggcatcgat gaatgcgaca gagttctccg ggatgtcaac
     4441 ggttccaagc cagtcctgat atcctgccag ccggagtgtc aggaaatgtt ctcctgctgc
     4501 aaggtttccc acggttgtgg ccgttgatcc gctgtaaaat ccgtcaatgt agatatctgc
     4561 tccaccagga ttactcttca cctggagtgc ccctatccgt gagagcggta acagggatgc
     4621 atcaacggtg atagtacccc cgggtggtac gttcacaata gtgaggtagg gttggaaccc
     4681 cgacagaaaa gcgcggacaa catggc
//
LOCUS       DAOOCO010000150         4644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_17462_length_4644_cov_6.581390, whole genome shotgun sequence.
ACCESSION   DAOOCO010000150 DAOOCO010000000
VERSION     DAOOCO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_4644_cov_6.581390"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK154_08320"
     CDS             complement(<1..217)
                     /locus_tag="PK154_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11099.1"
                     /translation="MAERRKEADACPDLRISEEPESSRKGADGEELPGNPYEIRYPGI
                     VALADENGTAIELIEFFDCIGGAMWSRH"
     gene            complement(345..1229)
                     /gene="fhcD"
                     /locus_tag="PK154_08325"
     CDS             complement(345..1229)
                     /gene="fhcD"
                     /locus_tag="PK154_08325"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HPW11100.1"
                     /translation="MEINGVPIDDTYAEAFPTWISRIIITAVNTEWARKAATEATGFA
                     TSAIGCPCEAGIESELRADQTPDGRPGVSILICAAKKKLKEQVVERLAECVLTSPTTA
                     VFDGLPQAAEKIAVKLHFFGDGYEYQKEVGGRKCWVIPIMEGEYIGEEEFGIVKGVAG
                     GNFFVMGENQMAALMGAQTAVEAINKVCGVITSFPGGVVASGSKVGSNKYKFMPASTN
                     EKYCPTIREKVPDTKVPAGIKGVYEIVIDGIDEKCVGEAMAKGIHAACTVPGVKYITA
                     GNFGGSLGPYKFELNKIL"
     gene            1874..2140
                     /locus_tag="PK154_08330"
     CDS             1874..2140
                     /locus_tag="PK154_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW11101.1"
                     /translation="MAFALHVNMERCTGCNNCVVACPVDALELFTIDPATNEKIYKVK
                     HGKAMVIDFKSELCAGCGVCVEACPHDVIRLAGRGSVTSEAKVG"
     gene            2174..3334
                     /locus_tag="PK154_08335"
     CDS             2174..3334
                     /locus_tag="PK154_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW11102.1"
                     /translation="MTLFPKFSKKRDGVNVIQEQKLLQQVNHLILNSEVCTGCGICVE
                     ACPEEAIALGLVGATRRGAVSYAAPVDIDEAKCSYCGVCVVLCPFNALTLKIDGEERL
                     PIVEKEGFPTYDMVADIDDEKCVRCTICEEVCPRDAIDRDVPEFEGTRPDGKERQLAI
                     KTKTTFKVDDEKCTLCGICGVLCPAITVKHKPFTAESGKVEGEVVWDETKCDACKVCV
                     EACPEECITVEREVESDKLDGQVDIDQKMCCTCTWCSRSCPTEAITVEKLFEGDIEFF
                     PEKCPGGCSTCVEICPANAIYLPSSVPASQLKGKVEPTIAVNKDFCILCGACVNACPG
                     EDIIRLHRTGIRVKGKETDLFNKIKEKLCTPRTSKVKEETPGSVQLKMMEKA"
     gene            3346..3927
                     /gene="hdrC"
                     /locus_tag="PK154_08340"
     CDS             3346..3927
                     /gene="hdrC"
                     /locus_tag="PK154_08340"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HPW11103.1"
                     /translation="MPRTKGYPEALEKKLADTRLYEDERHPEFTKTVEKLSMSIPHMC
                     YQCGTCTASCPSAPRSSYRIRKFVRRALLGLEDEALTDPDLWLCTTCYSCTDRCPRDI
                     APTDVIMAMRNLAFKRDIIPVNFLKTVQAIYTTGHGVPNNDVNRAARKKLGMNPEPPT
                     THAFPQYVKGIQKIIDYYELKKNADRIVKEREG"
     gene            3934..>4644
                     /gene="hdrB"
                     /locus_tag="PK154_08345"
     CDS             3934..>4644
                     /gene="hdrB"
                     /locus_tag="PK154_08345"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HPW11104.1"
                     /translation="MREYAFFLGCIAPNRYPGAESASYKTAEKLGIKLLDLEGASCCP
                     APGAFGSISLEIWYAMAARNLCLAEQMKKDIALICNGCYKSIWEVNHKLKQHKELKEK
                     VNEVLKTIDMEFKGTIDVWHLAELFYDPEIVGVQKIRDSVKVSLEGTRIAVHYGCHLM
                     KPRKERHFGTTEHPTWLEEMVGALGAEPVEYRNKMLCCGAGGGVRGYDLVHSLDITNE
                     KMINLKEARVDALTEVCPF"
BASE COUNT         1179 a         1185 c         1181 g         1099 t
ORIGIN      
        1 gatgcctcga ccacatggcg ccaccgatgc agtcaaaaaa ttcgatgagt tcgatcgctg
       61 ttccgttttc atcggcaaga gccacaatcc cgggataccg tatttcatac ggattgccgg
      121 gcagctcttc cccgtccgct cccttgcgcg atgactccgg ctcttctgaa atccggaggt
      181 ctgggcatgc atcagcttct tttctccttt ccgccatgag caaaaaacgc tcccgcgaga
      241 tgacagttct cacacccata ttttcgttgc cgaaaatatc gttaacaaaa taatcggcaa
      301 caagatattt aatcctgatt gaaccggatt ggggggagaa aaaattagag gattttgttc
      361 agttcaaatt tgtaaggacc aagagatcca ccaaagttac cggcggtgat atattttacc
      421 cccggaaccg tgcaggcggc atggatccct ttcgccattg cttcacctac acacttctca
      481 tcgatcccat cgataacaat ctcatacact ccttttattc ctgccggcac cttggtgtcc
      541 ggaaccttct cacggatcgt cgggcagtac ttctcattgg tactagccgg catgaatttg
      601 tacttgtttg aacctacctt ggaaccgctt gccacaacgc ccccggggaa actcgtgatg
      661 accccgcaga ctttattgat agcctccacc gcagtctggg ctcccatgag tgctgccatc
      721 tggttctcac ccatgacaaa gaaattgcct cctgcaacac ctttcacgat accaaattct
      781 tcttcaccga tatattcgcc ttccataatt gggatgaccc agcatttcct gccgccgact
      841 tctttctggt attcataccc gtcgccgaag aaatggagtt tgaccgcaat cttttctgcc
      901 gcctggggaa gaccatcaaa cactgcggtg gttggagagg taaggacgca ttcggcgaga
      961 cgctcgacca cctgctcttt cagcttcttc tttgccgcac agatcaggat cgacacaccg
     1021 ggtcttccat caggggtctg gtctgccctg agttcactct cgatcccggc ttcgcagggg
     1081 catccgattg ccgaggtcgc aaaacccgtg gcttcggtcg ctgctttccg ggcccattcc
     1141 gtgttcactg cggtgatgat gatccgggag atccatgtgg gaaacgcttc tgcgtaggta
     1201 tcatcaattg gtactccgtt tatctccata aaattcctct tttttatacc aaagatagta
     1261 ggatagtgct agaataaatt ttctaaatac ctctccctga aaggttcccc cctgaactgc
     1321 agaaaaaaga gatccctcat cagatgtgat cagcacaatt ggggcagaat tgaaacaggg
     1381 aattacgatt ttgtacaggg aagtgagatt ttttcaatga aatgccccat gataccgatg
     1441 gttcacatgc ctccctaatg aagatcgcgg atgcgttttg atgccagaat gcctcctgca
     1501 gtgtcatcct tgatgcccga ccgaaggagg gacatcgact ctttatatga aaatggtccg
     1561 gcaggtccgc cttgtatggt tctgtccctc aatgaaatca ggtaattttt ttttttttag
     1621 gaagacgggt aagggatatg tattatatat aatttcctat ttgatgttag tacttaacga
     1681 agcgcagcca gaataagcgg cgatttatca ttattatatt aattatgcgg attaataaac
     1741 aattttttat ttacactagt cctgatgaat cggcctgaat gcagggggga tttagacaag
     1801 tttatgtgta taatttaacc attcttttat agtcgatttt tgaatcgatc taaagctggt
     1861 gcgtgataca agcatggcat ttgcattgca cgttaacatg gagcgttgta ccggctgcaa
     1921 taattgtgtg gtcgcatgcc ctgttgatgc gcttgaactc ttcaccatag atcctgcgac
     1981 aaatgagaag atctacaagg tgaagcacgg gaaagcgatg gtaatcgact tcaaatccga
     2041 gctctgtgcc ggatgcggag tctgtgtcga ggcatgtcca catgatgtga tccggctggc
     2101 gggacgcggg tcggtaacat cggaggccaa agttggttaa cgttccgaac atggacaaac
     2161 ttgaggttat tgaatgacac tgtttccaaa attctcaaag aagcgggatg gcgtcaatgt
     2221 catccaggag cagaagcttc tccagcaggt gaaccacctg atcctcaact ctgaggtctg
     2281 caccgggtgc ggcatctgtg ttgaagcctg ccctgaagag gcaatcgcac tcggccttgt
     2341 cggtgccaca aggagaggcg cggtctcata cgcagcgccc gttgatatcg atgaggcgaa
     2401 atgttcctac tgtggagtct gcgtcgtctt gtgtccgttt aatgcactga cactgaagat
     2461 tgacggcgag gagcgtctcc ccattgtgga aaaagagggc ttcccaacat atgacatggt
     2521 ggcagatatt gacgatgaaa agtgcgtcag atgtaccatc tgcgaggaag tgtgcccgag
     2581 agatgccatc gatcgggatg tgcccgaatt tgaaggaacc cggcctgatg ggaaagaacg
     2641 tcagctggcc atcaaaacca agaccacgtt caaggtagac gatgagaagt gcaccctctg
     2701 tggtatctgt ggggtgctct gcccggcaat taccgtcaaa cacaagcctt tcaccgcaga
     2761 atcaggaaag gtcgaaggtg aagtggtctg ggacgagacg aagtgcgatg cctgcaaggt
     2821 ctgcgtggag gcatgccccg aggagtgcat caccgtcgag cgtgaggtcg agagtgataa
     2881 actggatggt caggttgaca tcgaccagaa gatgtgttgt acctgcacct ggtgttcgcg
     2941 gagctgcccg acggaggcaa ttaccgttga gaagctcttc gagggtgata tcgaattctt
     3001 ccctgagaaa tgccccggtg gatgttccac ttgtgtcgag atctgccctg caaatgccat
     3061 ttacctgcct tcctctgtac ccgcaagcca gctcaagggc aaggttgagc cgaccatcgc
     3121 agtcaataag gacttctgta tcctctgcgg tgcatgcgtg aatgcctgtc ccggcgagga
     3181 catcatcagg ctccaccgga ccggcatccg cgtcaagggc aaggagaccg atcttttcaa
     3241 taaaatcaag gagaagttgt gcactcccag gacctccaag gtcaaagagg agaccccagg
     3301 aagcgtacag ctgaaaatga tggagaaggc gtgaggggat tagacatgcc aagaacgaaa
     3361 ggataccctg aagcactcga aaagaaactc gctgacaccc gtctctatga agatgagcgc
     3421 caccccgaat tcacaaagac cgttgaaaaa ctctcaatga gcatccccca catgtgctac
     3481 cagtgcggga cgtgtacagc ttcatgccct tcagctcccc gcagctcata ccggatccgg
     3541 aagtttgtca ggagagcgct cctcggcctt gaagatgaag ccctcactga tcccgatctc
     3601 tggctctgca ccacctgcta cagttgcacc gaccgctgcc ccagggatat cgctccgacc
     3661 gatgtgatca tggcgatgag gaacctcgca ttcaagagag acatcatccc ggtgaacttc
     3721 ttaaagaccg tccaggccat ttacaccacc gggcatggtg tcccgaacaa cgatgtgaac
     3781 cgtgcagcac gcaagaagct tgggatgaac cctgaaccgc cgactaccca tgcattcccg
     3841 cagtatgtca aaggaatcca gaagatcatc gactactatg aactcaagaa gaacgcagac
     3901 aggattgtca aagagagaga ggggtgaaga ctgatgcgtg aatatgcatt tttcctcggc
     3961 tgtattgccc cgaaccggta ccctggcgca gaatccgcat cctacaagac agcagagaag
     4021 cttggcatca agctgcttga tcttgagggg gccagctgct gcccggcacc aggtgcgttt
     4081 ggttcaatat cccttgagat ctggtatgcc atggctgcac ggaacctttg ccttgccgag
     4141 cagatgaaga aggatatcgc cctcatatgc aacggatgct acaaatcaat ctgggaagtc
     4201 aaccacaagc tcaagcagca taaggagctg aaggagaagg tgaatgaagt cctcaagacc
     4261 atcgatatgg aattcaaagg gaccatcgat gtctggcacc tcgcagagct cttttacgat
     4321 cctgagattg tcggtgtcca gaaaatccgt gacagtgtga aagtttcact tgagggaacg
     4381 aggattgccg ttcattatgg ctgtcacctg atgaaaccaa ggaaggagcg acacttcggc
     4441 accaccgaac acccaacctg gctggaagag atggttggag cactcggcgc agaacccgtc
     4501 gaataccgga acaagatgct ctgctgcggc gcaggtggcg gggtacgagg atacgatctt
     4561 gtccactcac tggatatcac caatgagaaa atgatcaacc tgaaagaagc ccgcgtggat
     4621 gccctcaccg aagtctgtcc gttc
//
LOCUS       DAOOCO010000151         4560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_17856_length_4560_cov_4.173807, whole genome shotgun sequence.
ACCESSION   DAOOCO010000151 DAOOCO010000000
VERSION     DAOOCO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17856_length_4560_cov_4.173807"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PK154_08350"
     CDS             complement(<1..1054)
                     /locus_tag="PK154_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW11105.1"
                     /translation="MNDISVLTGGRAGDGINSAGLLIAHLFNQMGYQTYMYFDYPSLI
                     KGGHNFTITRAAEQKIGAHRDRCDYLLALSQDSVDLHRHLFSENTVILYDSGRVHGEG
                     QGIPVEAILKAEQAPPVMGNSCIIGAFAKSSGIPFDVVETVIRRQIPKAAAKNLVIAR
                     KGYEAVREERIIPRIGTTCCPLVTGNEAIGLGLLHGGLDAYVAYPMTPTSNLLHFLAR
                     AARNFPLTVVHPENEIAVMLMALGLSYAGKKTAVGTSGGGFCLMTEGFSLAGGSEIPI
                     VVVMGQRTGPSTGLPTYTGQTELGFVLHAGQGEFPRLVVAPGDPEQAYFWSSIALTLA
                     WKCQVPAVILADKTLCE"
     gene            complement(1145..1432)
                     /locus_tag="PK154_08355"
     CDS             complement(1145..1432)
                     /locus_tag="PK154_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HPW11106.1"
                     /translation="MGCIESLKYEIILRDASFTECRDYIRSSCREYVDVDPGFKIFDK
                     HIIGVPPISIGFEGDVIIFPFTKPCYGTFLMRIEDHDEAERIRKSASRKKK"
     gene            complement(1411..1893)
                     /locus_tag="PK154_08360"
     CDS             complement(1411..1893)
                     /locus_tag="PK154_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HPW11107.1"
                     /translation="MTNEQVHKEKSAVLVLGCPQVPVQTGIALYLISALRKKGVRTVI
                     AGNRAARTLVEVSDPERHYVGEVMDIDRYIGEIAETKRAFDYHFIFIHNDAGVSYAAT
                     VQAITGKTVVALIYGEHFQEIAGMITFPCEKIAAKAVHNPMPLKNKLDEVLPWVVSNL
                     "
     gene            complement(2032..2781)
                     /gene="fdhD"
                     /locus_tag="PK154_08365"
     CDS             complement(2032..2781)
                     /gene="fdhD"
                     /locus_tag="PK154_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPW11108.1"
                     /translation="MAGRRDEIMNTIRFTGIQVKEGKAVPIDDTVVKEERFSLFLNGS
                     HFTDLVASANQVEALGAGFVVCQGLSKEVNTVAVTGSEIRVRAPISGALEKEIATTGS
                     IGVKGCPPGKVVSDLSITIDEVYGITREIVTDLWRQTGGVHCSVLFHDGAIVARSSDV
                     GRHNTVDKVIGHAVLSRIPLGECVIGCTGRQPRDMVIKYANAGVPIVISRAATTDRGI
                     AAADEFGITLICFSRQERFTVYTHPSRIRGL"
     gene            complement(2754..3071)
                     /gene="cutA"
                     /locus_tag="PK154_08370"
     CDS             complement(2754..3071)
                     /gene="cutA"
                     /locus_tag="PK154_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPW11109.1"
                     /translation="MDSDPVIILSTAGEREARGIAQALVMQHLAACVNIIPVESVYRW
                     EGKLCEDREHLLIIKTVTSNADSVMHEIRNLHSYELPEMIVLPVSGGYHPYLQWLAEE
                     TRS"
     gene            3233..4381
                     /locus_tag="PK154_08375"
     CDS             3233..4381
                     /locus_tag="PK154_08375"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPW11110.1"
                     /translation="MYAERMGQLPPYLFARIDEMKAAKIRSGVDVIDLGVGDPDLPTP
                     GHIVEALCEAAHDPRNHHYPSYAGMETFRTAVAGWYRSRFSVGLDPGKEVLALMGSKD
                     GIAHIPEAFVNPGDYVLAPSPGYPVYRTSTLFAEGNVYEMPLLAKNQFLPDLSAIPEK
                     VAKAARLMFLNYPNNPTSAVTLPGFFEEAVDFALDQEILIVHDNAYSEIAFDGYQAPS
                     FLSTEGAMETAIEMHSLSKTYNMTGWRIGMACGNPDIIAGLARVKTNVDSGVFDAVQK
                     AAIAALTGPQDCIRDACRTYQERRDVLMKGLGALGFEVQAPKATFYVWMEVDDCMHFA
                     TRLLDEAGIVVTPGVGFGSGGDGYVRFALTRSTGRIQEAVDRMGELVS"
     gene            4378..>4560
                     /locus_tag="PK154_08380"
     CDS             4378..>4560
                     /locus_tag="PK154_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW11111.1"
                     /translation="MTLPDHMEVRNGHLCIREHDMVALAARHGTPLYVTDEDRIVGNY
                     RQYEQALRPYYPDTRIL"
BASE COUNT          971 a         1332 c         1188 g         1069 t
ORIGIN      
        1 cttcgcagag ggtcttgtct gcgagaatca ctgccgggac ctggcatttc catgcaaggg
       61 taagggcgat tgaggaccag aagtatgcct gctcgggatc ccccggggct actacgagcc
      121 gagggaactc tccctgccct gcatggagga caaatcctag ctccgtctga ccggtatagg
      181 tagggagtcc cgtggagggt ccggtgcgtt gccccatcac caccacaatg gggatttctg
      241 atccgcctgc gagtgagaat ccttcggtca tcaggcagaa tccaccccct gatgtcccga
      301 ctgcagtctt ttttcctgca tacgagagcc cgagcgccat cagcatcacg gcgatctcgt
      361 tctctggatg gaccacggta agagggaaat ttcgggctgc cctcgcaagg aaatgaagga
      421 ggtttgacgt gggggtcatc gggtaagcca catacgcatc gaggccgcca tgcaggagac
      481 cgaggccgat cgcctcgttc ccggtgacaa gcgggcagca ggtggtgccg atccggggga
      541 tgatccgctc ctcgcggacc gcctcgtacc ccttccttgc tatcaccaga ttcttcgcgg
      601 ctgccttcgg gatctgtctc ctgataaccg tctcgaccac gtcaaagggg attccggaag
      661 attttgcaaa cgccccgatg atgcaggagt ttcccatcac cgggggtgcc tgctctgctt
      721 ttaagatcgc ttctactggt atcccctgcc cctctccgtg aaccctgccg ctgtcgtaga
      781 gtatgaccgt gttttcagag aagaggtgcc ggtggagatc gacagagtcc tgcgagagcg
      841 cgaggaggta atcgcaccgg tccctgtgtg ccccgatctt ctgttccgct gcacgggtga
      901 tggtgaagtt atgtccccct ttgatgaggg aggggtaatc aaaatacata tacgtctggt
      961 atcccatctg gttgaagaga tgggcgatca gcaggcctgc actgttgata ccatcgccag
     1021 cccttccacc ggtgaggacc gatatatcat tcatctgctc cacctaccag tctggtgcac
     1081 accagggatg atatacattc cgcccattat cagattttaa aaagaattta ttcgatattt
     1141 cggattattt tttctttcgg gaggcagatt tccggatccg ttccgcctca tcatggtcct
     1201 cgatcctcat taagaacgtc ccgtaacagg gtttcgtaaa cggaaatatg atcacatccc
     1261 cctcgaatcc gatcgagatc gggggaaccc caatgatgtg cttgtcaaat atcttgaacc
     1321 cgggatcaac atcgacatac tctctgcagg aggacctgat ataatccctg cattcggtga
     1381 aacttgcatc gcggaggatg atctcgtact tcagagattc gatacaaccc atgggagcac
     1441 ctcatcgagc ttgttcttga gcggcatcgg attgtgaact gcctttgcag cgatcttttc
     1501 gcaggggaac gtgatcatgc ctgcaatctc ctggaaatgt tcgccgtaga tgagggccac
     1561 gaccgtcttc ccggtaatgg cctggacggt ggcggcataa ctgacccctg catcgttatg
     1621 gataaagatg aaatggtagt caaaggcacg tttcgtttcc gctatctcgc cgatataccg
     1681 gtcgatatcc atcacctcac ctacgtaatg ccgctcagga tctgaaacct ccacgagggt
     1741 acgtgcagcc cggttccctg caataaccgt ccttaccccc tttttcctta gggcactaat
     1801 caggtagagg gcgatcccgg tctggacggg gacctggggg cagccgagca cgaggaccgc
     1861 actcttctcc ttatgtacct gttcgtttgt catcttctgc acagaacctt tctcgctgtc
     1921 cctcaaatgg ttttccaaag gtctgttacc ggattttcac accaacagct tcgttttata
     1981 atgcgaccgt ccctctgcat cggtgctggc tcttccgatc tacagataat cctaaagacc
     2041 gcggatccgg gatggatgag tatataccgt aaaccgctcc tgccgtgaaa agcagatcag
     2101 cgttatcccg aattcgtcag cggcagcgat cccacggtcg gtggttgccg cacgggagat
     2161 gacgatcggc accccggcat tggcatattt gatcaccata tccctcggct gcctcccggt
     2221 gcagccgatg acacactccc cgagcgggat ccgggagagt accgcgtgtc cgatcacctt
     2281 gtctaccgtg ttatgcctcc ccacgtcgct gcttctcgca acgatcgccc cgtcatggaa
     2341 gagaaccgaa cagtggaccc cgccggtttg tcgccagaga tcggtcacga tctcgcgggt
     2401 gatcccgtac acctcatcga tggttatgga aagatctgat accaccttcc ccggtggaca
     2461 ccctttgacc ccgatcgatc cagtggtcgc aatctccttc tccaaggctc ctgatatggg
     2521 agcccggacc cggatctcac tgcccgtaac cgctacggta ttcacctcct ttgagagtcc
     2581 ctggcagaca acaaagcctg caccgagtgc ttcgacctgg tttgcgcttg cgaccagatc
     2641 ggtaaaatga ctgccgttca ggaacaggga gaaccgctcc tcttttacga ccgtatcatc
     2701 gatcggtaca gccttcccct cttttacctg tatcccggtg aaacggatgg tgttcatgat
     2761 ctcgtctctt ctgccagcca ttgcaggtat gggtggtatc caccggaaac cggcagtacg
     2821 atcatctccg gaagctcata ggaatgaagg ttccggatct cgtgcatgac tgaatcggca
     2881 tttgacgtta ccgtctttat gatcaggagg tgttccctgt cctcacacag cttcccctcc
     2941 catcggtaca ccgattcgac cggtatgata ttcacacacg ctgcaagatg ctgcatcacg
     3001 agtgcctgtg caatccccct cgcctccctc tcacctgccg tcgaaagaat gataaccgga
     3061 tcactgtcca tgaaacctca cctgtcccat cccttttttt ttcactccta attatgatat
     3121 cttcgatcca tcattccggg acacttcccc ttccttgcgt catcttcacc tcctggtcac
     3181 cttcacctcc tgaggtcact ttaaagaaca tacgaaagag attatatcca gcatgtacgc
     3241 ggaacgtatg gggcagcttc ccccgtatct ctttgcccgg atcgatgaga tgaaagccgc
     3301 gaagatccgg agcggggtgg atgtcatcga cctcggtgtc ggagacccgg atctcccaac
     3361 cccgggccat attgttgaag cactctgcga agcagcacat gatccgagaa atcaccatta
     3421 tccttcctat gcaggcatgg agaccttcag gacagcggta gccggctggt accggtcgcg
     3481 attctctgtc ggtcttgatc ccggaaaaga ggtacttgcg ctgatgggct caaaagacgg
     3541 tatagcccat atcccggagg cgtttgtcaa ccctggcgat tacgtccttg ccccgagtcc
     3601 aggctacccg gtctatcgga catcgaccct ttttgctgaa gggaacgtat acgagatgcc
     3661 gcttctcgca aagaaccagt ttcttcccga tctctcggca ataccggaga aggtagcaaa
     3721 ggcagcacgg ctgatgtttc tcaattaccc gaacaatccc acctccgcgg tgaccctccc
     3781 cggatttttt gaagaggctg tggactttgc cctggaccag gagatactca tcgtccatga
     3841 caatgcctat tccgagatag cgtttgacgg gtatcaggca ccgtcattcc tgtcgaccga
     3901 aggtgctatg gagacagcta tcgagatgca ctccctctca aagacttaca acatgaccgg
     3961 atggcgcatc gggatggcgt gtggcaaccc ggatatcatt gcaggtcttg cgagggtgaa
     4021 aaccaacgtg gactcagggg tctttgacgc tgtccagaaa gccgctatcg ccgcccttac
     4081 tggtccccag gactgcatcc gggatgcatg ccgcacctac caggaacggc gggatgtcct
     4141 catgaagggg cttggagcac tgggatttga agtccaggca ccgaaagcca ccttttacgt
     4201 gtggatggaa gttgacgact gcatgcattt tgccacccgg cttcttgatg aagccggtat
     4261 cgttgtcacc ccgggagtgg gattcggatc agggggtgac gggtatgtcc ggttcgccct
     4321 cacgcggtcg accgggcgca tacaggaagc agtcgatcgg atgggggagc ttgtttcatg
     4381 acactccctg accacatgga agtcaggaac ggccatctct gcatccggga acacgatatg
     4441 gtagcccttg ccgcccggca tggaactccc ctgtatgtga ccgacgaaga ccggatcgtg
     4501 gggaactacc ggcagtatga gcaggcactc cggccatact accctgatac ccgtatcctc
//
LOCUS       DAOOCO010000152         4521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_18075_length_4521_cov_6.846843, whole genome shotgun sequence.
ACCESSION   DAOOCO010000152 DAOOCO010000000
VERSION     DAOOCO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18075_length_4521_cov_6.846843"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK154_08385"
     CDS             <1..640
                     /locus_tag="PK154_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HPW11112.1"
                     /translation="VCTLPSSRPRLLENALFTLVATIVFILFAVRGYFDILSGIVFLV
                     LFALILLRMWKHNPDVVSSEVPTTRYPLLVTIAGLVMVVLGAELLLIGAGTIAELFAI
                     PPAVIGLSMVAVGTSLPELATSVVAAIQKRPGISIGNLLGSNIFNLLFVIGINSLFFT
                     IPVPDLKDIIVMLAFTLGIFILFIRKICETRIWGILLLGAYLMYILFLYGII"
     gene            788..1066
                     /locus_tag="PK154_08390"
     CDS             788..1066
                     /locus_tag="PK154_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11113.1"
                     /translation="MDLLLIFSVVIMALLILNTIVLVMRIRAAPDKPIGKGIGLVLGL
                     AIGLTLWIPLTYLFGSQSAGIIAGSITGIILAVALELYSAQIRKRLES"
     gene            1378..1539
                     /locus_tag="PK154_08395"
     CDS             1378..1539
                     /locus_tag="PK154_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11114.1"
                     /translation="MNICRSKVGIALADRTRELMKARKALYAAATIHTATTSMLIAWF
                     AVMLHVVLP"
     gene            2004..4397
                     /locus_tag="PK154_08400"
     CDS             2004..4397
                     /locus_tag="PK154_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW11115.1"
                     /translation="MIRPLSPEELRNSYDSTLVECKSTAELKPLEGIIGQERALKALT
                     FGLNIKESGFNIYVSGVHGTGRKVAVEKFLDEISRTRAKGNDWIYVNNFQNPYEPNSI
                     RLPPGMGIEFRNDMAAFIAEAKRVIPKSFESEDYVNRRNTALQSLEDEKAKLFAQIDA
                     SAREKNFVIQPGPQGLLTIPLKEGTPLEQEQFLALPDEEQREYQKKREELTVDMRNTF
                     RKLRELDQKGMETVDQLNRDVALGAMGHRVASLKDKYAHVPEVNAFIDAVQVDMVGNL
                     PQFMEEVQPQQPSPIPNPLLKELAFRKYEVNVIVDNSESSGAPIVFEQNPTYSNLFGK
                     VEREFQYGVVTTDFTMIRPGSIHKANGGFLVLPVEDLFRNPFVWDGLKSALKTGTITI
                     EEPAERMGFIATKSIKPESISLDLKVVLIGTPIINQILYTQDPDFSELFKVKADFDFA
                     MERNEDNVRKYSSFVCTICEKYNLRHLDRSAMAKIIEYGSRLADDKKKLTTRFSLISD
                     VLREASYYATLEEEGMVEARHITKAIDERTYRSSLIQEKIREYITRGVFLIDTEGEQV
                     GQVNGLSVISLGDIEFGRPSRVTASIGIGKGGIMDIEREASMGGPSHTKGVLILSGYL
                     ANKFAQDKPLSLNARLVFEQSYEGVDGDSASSTELYAILSALSELPLKQNIAVTGSVN
                     QRGEVQAIGGVNEKLEGFFEVCKAKGLSGNQGAMIPASNVQNLMLKEEIVEAAKAGKF
                     TIYPVTTIDEGIEVLTGMKAGKRLPDGTFEQGTVNYRVDKRLREMAQTYRGYQAPES"
BASE COUNT         1148 a         1135 c         1190 g         1048 t
ORIGIN      
        1 tgtgtgcacg ctcccctcct ctcgtccccg gctccttgaa aacgccctgt tcacgctggt
       61 tgccacgata gtatttatcc tctttgcggt caggggctat ttcgatatcc tgtcaggaat
      121 cgtatttctt gttctcttcg ccctgatact gctccggatg tggaaacaca atcctgatgt
      181 ggtctcctcc gaggtaccga caacccggta tccccttctc gtgaccattg caggactggt
      241 aatggtggtc ctcggtgccg agctgctcct cataggagcc gggacgattg ccgagctatt
      301 tgcgattcct cccgcggtga taggtctttc catggtagca gtcggaacat ccctgccgga
      361 actcgcaaca tcggttgttg cggcaataca aaagcgcccg ggtatctcaa tcgggaatct
      421 tctcgggagc aacatcttca acctcctctt tgtcatcggg attaattcgc tttttttcac
      481 cattcctgtc cctgatctga aggatatcat cgtgatgctt gcattcacgt taggaatttt
      541 tatcctgttc attcggaaaa tatgtgaaac acggatatgg ggtatcctgc tccttggggc
      601 ttatctcatg tatatcctgt ttttatatgg aatcatctga atcatccggc tgctgggatc
      661 atccatcata cgtgaggtcc tccttctctt tcgagaagaa aaggaatggt gattcaacga
      721 gaaaaagtgt cgagttgcac gtgcgtctca cgggaattct tatctttgcg gcagttgacc
      781 tctattcatg gatcttctcc tgatattttc agtcgtcatc atggcgctgt tgatcctcaa
      841 cacgatcgtc ctcgtcatgc gaatcagggc tgcacccgac aagccaatcg ggaaggggat
      901 aggcctcgtc ctcggtctgg ctatcgggct caccctctgg atacctctga catacctctt
      961 tggatcccag agcgcaggga tcatcgcggg atcaataaca gggatcatcc tggctgtcgc
     1021 cctggaactc tactcggccc agatacggaa gagactggaa tcgtagtgct tctgacactc
     1081 ccttctattt ttcgttgaag atccaagcag cgagcgggaa ctcgtctcgg aaaaggcacg
     1141 caaaaaagat caatgaaagg aggtagtagt cggtcccgtg atcccggaaa aaacaggctg
     1201 tttctcattt ctctctataa aggatttgag gtcattatgg ggaccaatgc gacaaatgag
     1261 tgaactgccc ggttcattcc tgaaaccagg ttcatggcac cattatttta atgccggttc
     1321 aaaggtggta ggcagggtga tatccgttca ggatggatac ccagggagat aacaacaatg
     1381 aatatttgta ggagtaaggt cgggattgca ttggcagatc gcacacgaga gctgatgaag
     1441 gcaagaaaag ccctttatgc tgccgcgacg atccataccg caacgacctc catgcttatc
     1501 gcatggtttg ctgtcatgtt gcatgtagtc ctgccctgat acaatcacaa ccgcccgttc
     1561 ggaaccagca cgtatcccgg acgggcggaa aatatccttt atccgccttt gccatgttca
     1621 gacaggagaa ctattgagaa gaacaaaaag ctccctgtca tactctctca tgaggagatg
     1681 ccccggtatt ctggtgcgta atctttctcc atcgaataat cccatgatgt cagaaacgct
     1741 tagtccatag tttttccctc ttcccgttga aatcctgtat tcccaccggc gggagctatt
     1801 gtgcggggag atcgttgtgt acttttcaca ctaccttgaa atggaagagg tttcctgaac
     1861 cggggatcac tctgccggat ggtgacaaag gagagcagga ggatttttga aacttcagac
     1921 ctgaaaggta ttatttcaaa ttccaccaca ttcccataga ctaataaggg gagaggtata
     1981 tcgctccctt atctggtgct aatatgatca ggccactctc cccagaagag ctacgaaata
     2041 gctatgattc cacgcttgtg gagtgcaaaa gtaccgcaga actgaaaccc cttgagggaa
     2101 taatcggcca ggagagggct ctgaaagcac tcacgttcgg tctgaatatc aaggagagtg
     2161 gattcaacat ctatgtgtca ggggtccatg gtacaggacg aaaagttgcc gtggaaaaat
     2221 tcctcgatga aatttccagg acccgggcta aggggaatga ctggatctac gtgaataatt
     2281 tccagaatcc gtatgagccg aactccatca ggcttcctcc agggatgggg atcgaattcc
     2341 ggaatgatat ggctgctttc atcgccgaag caaaacgggt catcccgaaa agttttgaga
     2401 gcgaagatta tgtaaaccgt cggaatacgg cgctccagtc tctcgaagac gagaaggcca
     2461 agctctttgc ccagatcgat gccagtgcca gggagaagaa ttttgtcatc cagcccgggc
     2521 cccaggggct gctgacgatt cctctcaagg aggggactcc cctggaacag gaacaattcc
     2581 ttgccctgcc ggacgaggaa cagcgcgagt accagaagaa gcgggaggaa ctcacggtcg
     2641 atatgcgaaa cacgttccgg aagcttcgcg aactcgacca gaaagggatg gaaacggttg
     2701 atcagctcaa ccgtgatgtg gcactcgggg ccatgggcca ccgggtagcc tcgctcaagg
     2761 ataagtatgc tcatgtccct gaagtgaatg cttttattga tgcggtacag gtggacatgg
     2821 tcgggaacct ccctcagttc atggaagagg tccagcccca gcagccttcc ccgatcccaa
     2881 atccgctgtt aaaagagctt gcgttccgga aatacgaagt gaacgtcatt gtagataact
     2941 cggagagcag cggggccccg atagtgttcg agcagaaccc cacctactcg aacctcttcg
     3001 gaaaagtcga gagggaattc cagtatgggg tggtaacaac ggatttcacg atgatccggc
     3061 cgggctcgat ccacaaggcc aacggaggat tcctggtgct gcccgtggaa gatctcttca
     3121 gaaatccgtt tgtatgggac gggctcaagt ccgccctcaa gacagggacg atcaccatag
     3181 aagagccagc ggaacggatg ggattcatcg caacgaagag catcaagcct gaatccatct
     3241 cccttgacct gaaggtcgtc ctgatcggca ccccgatcat caaccagatc ctctatacgc
     3301 aagatcccga tttctccgag ctcttcaaag ttaaggccga ttttgacttc gccatggaaa
     3361 ggaatgagga taatgtgcga aaatactcgt ctttcgtctg taccatctgc gaaaaatata
     3421 acctcagaca ccttgaccga tcagccatgg ccaagattat tgaatatggg tcccgtcttg
     3481 ccgatgacaa aaagaaactc accacccgct tttcgctgat ttcagatgtc ctccgggagg
     3541 caagttatta tgcaaccctt gaagaggagg ggatggtcga agcacggcat attacaaagg
     3601 caatcgacga gaggacctac cggtcgagcc tgatccagga aaaaatccgg gaatacatca
     3661 cccgcggtgt cttcctgatc gacacagaag gggagcaagt ggggcaggtg aacgggcttt
     3721 ctgtcataag tcttggtgac atcgagttcg gaaggccatc cagggtcacg gcaagtatcg
     3781 ggattgggaa ggggggcatc atggatatcg agcgtgaagc gagcatgggt ggcccgagcc
     3841 ataccaaggg agtgctgatc ttgtcagggt atctcgcaaa caagtttgcc caggacaagc
     3901 cactgagtct caacgcccgg cttgtgttcg aacagagcta tgaaggggtt gacggggaca
     3961 gtgcatccag taccgagttg tatgccatcc tctcggccct ctctgaactt cccctcaaac
     4021 agaacattgc ggtcaccggg tcggtgaacc agagagggga ggtccaggcg ataggggggg
     4081 tcaacgagaa actggaggga ttcttcgagg tctgcaaggc caaagggctc tccgggaacc
     4141 agggtgccat gatacccgca agcaatgtcc agaacctgat gctgaaagag gagattgtgg
     4201 aggcggcaaa agcagggaag ttcacgatct acccggtcac gaccatcgac gagggcatcg
     4261 aggttctcac cggaatgaaa gcagggaaac gactgcctga cgggaccttt gaacagggaa
     4321 ctgtcaacta ccgtgtcgat aaaaggctcc gggagatggc acagacctat cggggatacc
     4381 aggcaccgga atcctgatca ctaccaattt ctgctggaga ttgctgatcc agatccccag
     4441 gtggatctca aaattcagtc atttctattt atataccaga aagagatacg atactgcatg
     4501 acacctgaac cctggaagag a
//
LOCUS       DAOOCO010000153         4466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_18361_length_4466_cov_6.175017, whole genome shotgun sequence.
ACCESSION   DAOOCO010000153 DAOOCO010000000
VERSION     DAOOCO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18361_length_4466_cov_6.175017"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK154_08405"
     CDS             complement(<1..285)
                     /locus_tag="PK154_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11116.1"
                     /translation="MDQEVFIARQEQDLAARIPGVLQQRKDLGLEGLVHGLDSVIIST
                     EPGRLELSAEELLRYTGLTLEAHFEDDSFTTLVLGTKGSASFLLRARKKGE"
     gene            complement(397..540)
                     /locus_tag="PK154_08410"
     CDS             complement(397..540)
                     /locus_tag="PK154_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPW11117.1"
                     /translation="MAIDPICKMTVDEKTAKFTSTFKGKVYYFCAPGCKKKFDADPAK
                     YAD"
     gene            complement(551..2980)
                     /locus_tag="PK154_08415"
     CDS             complement(551..2980)
                     /locus_tag="PK154_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPW11118.1"
                     /translation="MKGMRKADIRISGMHCATCAVTIEDAVGSLGKGTTAKVNFGTDI
                     ARIEFDPDLVSLREIEAVIKGAGYDVIHDEVIIRVGGMMCATCVETIEKALSSFPGVI
                     HARVNLATEKAYVTYNPSVTSIEEMKGAIENAGYQYLGVAEDLSLEAEERARERDLSD
                     KFRRFSIGFAVSIPLFLLMFIPLPVGMHTLSWFMLVVSTPVFFYVAYPIFRAALSALR
                     NRTLTMDVMYAMGTGVAYGASVLGTLGIVLTHDFMFYDTAIMLASFLILGRYLEARAK
                     GRTSEAIRKLVGLRPRTALVLRDGNETEIAIEDVAAGDLVIVKPGGKIPVDGEVTDGE
                     SYVDESMITGEPIPVLKSKGSKVVGGTLNTNSIFTFRTEKVGKDTVLSQIITLVEEAQ
                     ATKPPVERIADVAVSYFIPLVLTIAISAFLLWYLVLDSSLLFALTALISVLVVACPCA
                     LGLATPTAVTVGVGRGAELGILIRNGEALEVADRITTVVFDKTGTLTRGKPEVTDLIP
                     FGIDERTLLSLVSSVEKNATHPLAEAVVRKAEELHVPVESSTEFTTFAGKGVAARVLG
                     EEVLVGNRVIFAERNIIIPPELDQQIIRLEETGRTVVLVAVAGNNAGVIAIADTVKKT
                     TPDAIQELKDMGLSVAMITGDNKRTAEAVGGQVGIGRVIAEVLPEDKEREVKSLQAAG
                     SVVAFIGDGINDAPALARADVGIAIGSGTDVAIESGDIVLIKDDLLDVAAAIQLSRKV
                     MSRIKQNIFWAFAYNALLIPLAAGILYPFTGYTFSPVLAALAMAASSVTVVSLSLLLK
                     RYIPPAKKGRA"
     gene            3113..4072
                     /locus_tag="PK154_08420"
     CDS             3113..4072
                     /locus_tag="PK154_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPW11119.1"
                     /translation="MKEPNPFQVILFFLLVAPGVYLLYAWGVGTGPATFLWVGIIAAL
                     VALLIAWSFKIADQWEKAVVLRLGRFTGLRGPGPFFIIPFAERVAYWIDLRVITTSFR
                     AEKTLTKDTVPVDVEAVLFWKVLDPERAALEVEDYRTAISWASQTALREVIGKSFLSE
                     MLEGREKLDAVLQKIIDTRTEQWGIHVSSVEIRDVIIPPELQDAMSMQAQAERERQAR
                     VILGDSERQIAEKFEEAARTYEHNPTALHLRAMNMLYEGLKVKDNATLVIVPASILET
                     MNLGSTTGLVSLAKLQEMEKKRQQSGSGAEDPARANEEKKLPE"
     gene            complement(4109..>4466)
                     /locus_tag="PK154_08425"
     CDS             complement(4109..>4466)
                     /locus_tag="PK154_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HPW11120.1"
                     /translation="SVFDTGFFQSVIDIQTVTSVPVIMVVSVVISQFISNVPFVALFQ
                     PLIIQQGMSIQAMLALAAGSTIAGNLTILGAASNVIVIQNAERCGVTLTFLEFFKIGL
                     PLTVLNVIVYTVFLSF"
BASE COUNT         1076 a         1192 c         1144 g         1054 t
ORIGIN      
        1 ttcgcctttt ttccttgccc tcaggagaaa ggaggcagaa ccttttgtcc caagcacaag
       61 ggtcgtgaat gaatcatcct caaaatgtgc ttccaaagtg agtccggtat accggagaag
      121 ctcttcagca gatagttcaa gccgtccggg ctcggtgctg ataatgacgg agtcaagacc
      181 atgaaccagc ccttcaagcc ccagatcctt cctttgctga agaaccccag ggatcctggc
      241 tgcaaggtcc tgctcctgcc gggcaatgaa aacctcctga tccattgcat ctctttcctg
      301 gaacccatct tctccgggag tttctttctc tgcgatgaag aggggaagca tgcacatatt
      361 attgttggca atcgagaagg ggggtgacga ccccacctaa tccgcgtact ttgcagggtc
      421 tgcatcgaat tttttcttgc atcccggggc acagaaatag tacactttcc ctttgaacgt
      481 gctcgtgaat tttgcggtct tttcatcaac ggtcatttta cagatcggat cgatggccat
      541 cggtttcacc tcaggctctc cctttctttg caggagggat ataccttttc aataaaaggg
      601 aaagggatac aacggtaact gaactagccg ccattgccaa tgcagcgagt accgggctga
      661 atgtgtaacc ggtaaaagga tataaaattc cagcggcaag ggggatgagc agtgcgttgt
      721 acgcaaacgc ccagaaaatg ttctgcttga tacgactcat aactttccgg gaaagctgaa
      781 tagcggccgc gacatccaga agatcatcct tgataagcac gatatccccg ctctcgatag
      841 caacgtcggt cccgctccct attgcgatac ctacgtctgc ccgggcaagg gctggagcat
      901 cgttaatgcc atccccgatg aatgcaacca cagatccggc tgcctggagc gatttcactt
      961 cccgctcttt gtcttcaggg agtacttctg caataacccg gccgattccc acctggcctc
     1021 caactgcttc cgcggttctc ttgttgtctc ctgtaatcat cgctaccgaa agccccatgt
     1081 ctttgagctc ctgtattgcg tccggagtcg ttttcttgac ggtgtcagca atagcaataa
     1141 ctccggcatt gttccctgca actgcgacca ggacaacggt cctgccggtc tcttcgagcc
     1201 gtataatttg ctggtcgagc tcaggaggta tgataatatt tctctcggcg aagatcaccc
     1261 ggtttcccac aaggacctct tctccgagaa cccgggcagc gacacctttt cccgcaaagg
     1321 tggtgaactc cgttgaagac tccacaggaa catgcaactc ctctgctttt ctgacgactg
     1381 cctcggcaag ggggtgggtt gcattcttct ctacgctact cacaagggat aacagggttc
     1441 gttcatcaat tccaaatggg ataaggtctg tcacctctgg ttttcctctt gtcagggttc
     1501 ctgtcttgtc aaagaccacc gtggttatgc gatcggcaac ttccagagct tcaccgttcc
     1561 tgatgaggat cccgagctct gctcccctcc cgacaccgac cgtaaccgcc gtgggagttg
     1621 caaggccgag ggcacagggg caggcaacaa cgaggactga aatgagggcg gtgagtgcga
     1681 agaggaggga ggaatccaag acgagatacc acaggagaaa agcactgatg gcaatggtca
     1741 gcactagtgg gataaaataa gagaccgcaa catcagcgat tcgctcaacc ggaggctttg
     1801 ttgcctgggc ttcctcaaca agggtgatga tctgggagag aacggtatcc ttgccgactt
     1861 tttctgtcct gaacgtgaag atgctgtttg tgttgagggt ccctccgact accttggagc
     1921 ctttcgattt caggactggg atcggttcac cggttatcat cgactcatca acgtagctct
     1981 ctccatcagt tacctccccg tctacaggta tctttccgcc gggtttgacg attacaagat
     2041 ctcccgctgc aacatcctca atcgcaatct cggtttcgtt tccatcgcga aggaccaggg
     2101 cagttcgcgg ccggagaccg accagtttcc ggatggcttc agaagtgcgt cccttggcac
     2161 gggcttccag atatcttccg aggattagaa atgatgcgag catgattgcc gtgtcataga
     2221 acatgaaatc gtgggtcagc acgataccga gggttccgag aacactcgcg ccataggcaa
     2281 cgccggtacc catcgcatac atcacatcca tggtaagggt ccggttccgg agtgcactca
     2341 gggcagcacg gaaaatcggg taagccacgt agaaaaatac cggggtggag accaccagca
     2401 tgaaccagga gagcgtatgc attccaacag gaagggggat aaacatcagg aggaaaaggg
     2461 ggatactcac tgcgaaaccg atagaaaatc gccggaactt gtcggaaagg tcccgttccc
     2521 gggcacgttc ttcagcctcc agtgagagat cttctgcgac tcccaggtac tggtagccgg
     2581 cattttcgat cgctcccttc atctcctcaa tggaggtaac agagggattg taagtgacat
     2641 atgccttctc tgtggcaaga ttgacccgtg catgaatgac cccggggaag gatgagagtg
     2701 ctttttcgat agtttccacg caggtggcac acatcatacc ccccacccgg atgatcacct
     2761 catcgtgaat gacgtcatat cctgcaccct tgatcactgc ctcaatttcc cgcagggaga
     2821 ccagatctgg atcaaactca atccttgcaa tatcagtacc aaaatttacc tttgcggttg
     2881 ttcccttacc gaggctgccc accgcatcct ctatggttac cgcgcaggta gcacagtgca
     2941 tccctgatat tctaatgtcg gctttcctca tccctttcat aacgccccac aacgcatgtt
     3001 gtacccagat tgacggtatc atgggtaata attactgctc gttgccggga ctctctcttt
     3061 taggatttcc accatgtcaa ggtatatcac ctcgcctgtt acagtgtcgg atatgaaaga
     3121 accgaaccct ttccaggtca ttctcttttt cctgcttgtt gcaccagggg tgtatctcct
     3181 gtacgcatgg ggagtgggaa caggtcctgc cacgttcctc tgggtgggaa ttattgctgc
     3241 cctcgttgcc ctgcttatcg catggtcatt caagattgca gaccagtggg agaaagcagt
     3301 tgttctcagg ctcggccgat ttaccggtct tcggggtccc ggtccttttt tcatcatacc
     3361 tttcgcagaa agggtcgcgt actggatcga tctgcgggtg atcacgacct cgttccgtgc
     3421 tgaaaaaacg ctgacaaaag atactgtccc ggtggatgta gaagcggttc tcttctggaa
     3481 agtgcttgat ccggaaaggg ctgcgcttga ggtggaggat taccggaccg caatcagctg
     3541 ggcatcccag acggcccttc gcgaggttat cgggaaatcc ttcctttcag agatgctgga
     3601 agggagggag aagctcgatg cagtgctcca aaagatcatt gataccagaa ccgagcaatg
     3661 gggtatccac gtcagttcgg tagagatacg cgatgtgatc attccccctg agcttcagga
     3721 tgcgatgtcc atgcaggcgc aggcagagcg tgaacgccag gcccgggtca tccttggtga
     3781 ttcggaacgg cagatcgccg agaagtttga ggaggcagca aggacctacg aacacaatcc
     3841 aactgcactc catttgcgcg cgatgaacat gctctacgag gggctcaagg tgaaggacaa
     3901 tgccacgctc gtaatcgtcc cggcatcgat ccttgagaca atgaacctcg ggtcaacaac
     3961 cggccttgtc tctcttgcaa aactacagga aatggaaaag aaaagacaac aatcggggtc
     4021 cggggcagag gaccctgccc gggcaaatga ggaaaagaaa ttaccagagt aaaacaaaac
     4081 tcccttttct atgccttatc atgccctgtc agaatgacag aaacactgtg tatactatga
     4141 cgttcaggac cgtcaaggga agcccgattt tgaaaaattc aaggaatgtg agtgtcaccc
     4201 cgcaccgttc cgcattctgg attacaataa cattactggc ggcaccaagg atggttagat
     4261 ttccggctat tgtacttccg gcagcaaggg caagcatcgc ttggatcgac atcccctgct
     4321 gaatgatcaa cggctggaaa agagcaacga aggggacatt cgagataaac tggctgatca
     4381 ccacacttac caccattatg acggggaccg aggtgactgt ctggatatcg atcaccgatt
     4441 ggaagaaccc tgtgtcaaag acgctc
//
LOCUS       DAOOCO010000154         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_18441_length_4452_cov_5.903116, whole genome shotgun sequence.
ACCESSION   DAOOCO010000154 DAOOCO010000000
VERSION     DAOOCO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18441_length_4452_cov_5.903116"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK154_08430"
     CDS             complement(<1..612)
                     /locus_tag="PK154_08430"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HPW11121.1"
                     /translation="MNGQPSFREAVSARGGVYVIGVAGDSGSGKTSFTQSIRQLLGEE
                     LVSTITLDDYHLLDREQRKERDITPLAPEANDLGGLEKDVASLKQGAAIEKMVYNHST
                     GTLEGPVLFRPSKIVILEGLHPLYTSTLRSLIDFSLYVDPADDVKKEWKLKRDMEKRG
                     YTRHEVMEEIQKRQADYHRYIAPQREYADAVIHIGFSRYGRDLG"
     gene            complement(686..1894)
                     /locus_tag="PK154_08435"
     CDS             complement(686..1894)
                     /locus_tag="PK154_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPW11122.1"
                     /translation="MTVRELGHDIYWIGAIDWNRRLFDSLIPLPEGTSYNAYYVKGSE
                     KSALIDTVDPTKEFEFVCNLIKLGVEKVDYIVINHAEQDHSGSLPMASELFPEARIVV
                     SEKCKDLVVSLLEIAPERCLVVKDHDTISLGDRTLEFIMAPWVHWPETMLTYLREDKI
                     LFSCDLFGSHIATSTLVVHDAAEMYRPAKRYYAEIMMPFRMQIRDHMKKIADLKIEMI
                     APSHGPLYQPPDPILALYSDWISDTVKNEVVIPYASMHGSTEKMVLHLAGALIDRGIA
                     VKPFDLTKTDLGELAAALVESATIVIGTPTLLFGPHPLVANAAFITNLIRPKAKYATV
                     IGSYGWGGKTVEAVKGMLNHLSAEIIDPVYVKGAPDSACLRELEALAERIAAKHREAG
                     LLNDSPPPLP"
     gene            complement(1891..2028)
                     /locus_tag="PK154_08440"
     CDS             complement(1891..2028)
                     /locus_tag="PK154_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11123.1"
                     /translation="MGITSYEDGSIDSEPEGCRATSGVALVQSCCRQGESRWVLEETL
                     P"
     gene            2372..2926
                     /locus_tag="PK154_08445"
     CDS             2372..2926
                     /locus_tag="PK154_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HPW11124.1"
                     /translation="MKDTDEAVRLVVSLMAVSARTAPKGKGKDSIMTRTVLNSDRSLL
                     SLEMIRYGNEHAQPFFVRDGKNIAASDACLLIGVRGYEPLGLNCHGCGFSSCAEMEAF
                     RDQSPVKGPPFAGPNCIMKMTDLGIAIGSAAKTASLHNLDNRIMYTAGVAGLSLGWME
                     GCSVAYGIPVSVTGKNIFFDRPVG"
     gene            2988..3617
                     /locus_tag="PK154_08450"
     CDS             2988..3617
                     /locus_tag="PK154_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW11125.1"
                     /translation="MRIRDLILPEDKVFFTLFKEMAEKIDEAAGILNEITHELPAGTE
                     KGQKVRQIEHAADEITRQTYERLNDSLITPLEPEEIARLAPALDDVLDRIDWVTHQIC
                     NYGIPQSTELLREFSYLIILSSAEITHGIDDLATMKRSEEIEQHAREINRLYNVSNEL
                     LSRAVIELFQSKDPLLIIKLKDIYEGMARVMEKYNDVGHALTDISRNHA"
     gene            3623..>4452
                     /locus_tag="PK154_08455"
     CDS             3623..>4452
                     /locus_tag="PK154_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW11126.1"
                     /translation="MDLLIITGIVLALMLNFVNGVNDASHSIATVVETRALSPLKASV
                     SSAIANFIGPFVFTTAVAATIGTALVVPGAMTPVSLVVAMLVSIMLILAATHIGLPVS
                     SSHALIGGILGTGVAVAGLSALILPPAAMIRALVLFSLTGAGIGALILSILAYYLEEG
                     IRPALVLGVIFGISVTIPLMILAGLLVLSGILAIIVFIFISPVIGFIAAFLFDLLVSH
                     LFRFSRQNRMRRIFQPLQVAASLFQAAGHGANDGQHAVGVICALLLAGGLIPLFMVPL
                     "
BASE COUNT          900 a         1263 c         1227 g         1062 t
ORIGIN      
        1 accgagatca cgtccatagc gtgaaaaccc gatatggatc acggcatcgg catactctct
       61 ctggggtgca atgtaccggt ggtaatcggc ctgccgcttc tggatctctt ccatcacttc
      121 atgccgggta tagccgcgct tttccatatc cctcttcagt ttccattcct ttttcacatc
      181 gtctgccggg tcgacataga gggagaaatc aatgagcgaa cggagggtac tggtatacag
      241 gggatgcagg ccttccagga tcacgatttt ggaggggcgg aacaggaccg gcccttccag
      301 tgtgccggtc gaatggttgt acaccatttt ctcgattgcc gccccctgct tcagggacgc
      361 cacgtccttt tcgagccctc cgagatcatt tgcctcgggc gcgagcgggg tgatatcccg
      421 ttccttccgc tgttcccggt cgaggagatg gtagtcgtca agggtgatgg tcgagaccag
      481 ctcctctccc agcaactgtc ggatcgactg ggtgaagctg gtcttccctg aaccggaatc
      541 ccctgcaaca ccaataacat acacgccgcc ccgggcagaa acggcttccc ggaatgaggg
      601 ttggccattc attttcccgg gctcctttgg tgattccccc ctcccaggag taccaggtac
      661 tgggttcttt cgaatcgttc cttgctcatg gcagcggagg aggtgaatcg ttcagcaggc
      721 ctgcttcccg gtgctttgca gcaatccgtt ctgcgagggc ttcgagttcg cgcaggcagg
      781 cagagtcggg ggctcccttc acatagacgg gatcgatgat ctcggcagaa aggtggttca
      841 gcattccctt gactgcctcc acggtctttc ccccccaccc gtacgacccg attaccgtcg
      901 catatttcgc tttcggcctg atcaggttgg tgatgaatgc ggcattggcg acaagaggat
      961 gcggcccgaa gaggagggtc ggggtgccga tgacgatggt ggcagattcg accagagcgg
     1021 cggccagttc gccaaggtcc gtttttgtca ggtcgaatgg tttgactgcg ataccgcgat
     1081 cgatgagagc cccggccagg tgaagcacca tcttctcagt gctcccgtgc atgcttgcat
     1141 acgggatgac gacctcattc ttcacggtat ccgagatcca atcactgtaa agcgcaagga
     1201 ttggatcggg tggctggtac aggggaccgt ggctgggtgc gatcatttcg atcttgaggt
     1261 cagcaatctt cttcatgtgg tcccggatct gcatccggaa cggcatcatg atctcagcat
     1321 agtaccgttt tgccgggcga tacatctccg ccgcatcgtg caccaccaga gtgcttgttg
     1381 cgatgtggga accgaacaga tcgcatgaga acagaatttt atcctcccgg agataggtca
     1441 acatggtctc cggccagtgg acccaggggg ccataataaa ttcgagggtc cggtcgccga
     1501 gggatatcgt gtcatggtct ttcaccacga gacaccgttc cggggcgatc tcaagcaggc
     1561 tcaccaccag atctttgcat ttctcgctga cgacgatccg ggcttccgga aagagctctg
     1621 aagccatggg aagcgatcct gagtgatcct gctctgcatg gttgatgacg atataatcca
     1681 ctttttcaac accgagcttg atcaggttgc agacaaactc gaattccttg gtcggatcaa
     1741 cggtatcgat aagggcggac ttctcgctcc ccttcacata gtaggcattg taacttgtcc
     1801 cctccgggag agggatcagg gagtcgaaca gtctccggtt ccagtcgatc gccccgatcc
     1861 agtagatgtc gtgacccagt tctcgtacag tcatggtagc gtctcctcaa gcacccatcg
     1921 gctctccccc tgccggcagc aggactgcac aagggctacc ccggatgttg ccctgcagcc
     1981 ttcaggttcg gagtctatgg atccgtcttc ataggacgtg attcccatgc cggtataacg
     2041 gtttctatcg cagcacagga agaggcgaac tggaacgtct cactggggca gtgggcaggg
     2101 agagtatcag gcgaattcgc gatcgtattg ttgaagagga tcgggttgct ccagctgccg
     2161 gacaacttcc taaggatcag ggtctggtcg ccggctggtc tcttaattca gggaagggaa
     2221 actcggatct tcctggttcg gatccctttt tattgatcgg atcccttttt attgatcgga
     2281 tcccttttta ttgacctgat ccctttttat tgatcggatc cctttttgtt gaccggatcc
     2341 cttttcattg accggatccc tacgtacgat catgaaggat accgatgaag cggtccggct
     2401 ggtcgtctcc cttatggcgg tatcggcacg gactgcacca aaggggaagg ggaaggactc
     2461 gatcatgacc cgcacagtcc tgaactctga tcgttccctg ctttcgctcg agatgatccg
     2521 ttacgggaac gagcatgccc agcctttttt cgtccgtgat ggtaagaata tcgctgcatc
     2581 cgatgcatgc ctcctgatcg gagtccgggg atatgagcca ctgggcctga actgccatgg
     2641 gtgcggtttc agctcctgtg ccgagatgga ggcgttccgg gaccaaagcc cggtcaaggg
     2701 accaccattc gcggggccaa actgcatcat gaagatgacg gacctcggta tcgccatcgg
     2761 gtctgccgca aagaccgctt ccctgcataa cctggataac aggatcatgt atacggccgg
     2821 agttgcaggc ctctccctcg gttggatgga gggctgcagt gtcgcctatg ggatcccggt
     2881 gagtgtcacc ggaaagaaca tcttcttcga caggccggta ggataaatca cctcagcctt
     2941 tccgggcacc agttttttct gtttccgggt accaggaaag agtacctatg cggatccggg
     3001 acctgattct accggaagat aaagtcttct tcaccctctt taaggagatg gctgaaaaga
     3061 tcgatgaagc agcagggatt ctcaacgaga tcacccacga actgccagca ggaacggaga
     3121 aagggcagaa agtccggcag atcgagcatg cagccgatga gatcacccgg cagacctacg
     3181 agcggctgaa cgattcactg ataacgccac tggaacccga ggagattgcc cgccttgctc
     3241 cggcacttga cgatgtgctc gatcggatcg actgggttac ccatcagatc tgcaattacg
     3301 gaatacccca gtccacggaa ctcctgaggg aattctcgta cctgatcatc ctttcaagcg
     3361 ccgagatcac gcacggaata gacgatcttg ccaccatgaa acgatcagag gagatcgaac
     3421 agcacgcacg tgagatcaac aggctgtaca atgtatcgaa cgaacttctc tcgagagcgg
     3481 tgatcgagtt gttccagtca aaagatccgc tcctcatcat caagctcaag gacatctacg
     3541 agggaatggc aagggtgatg gagaagtaca acgatgtcgg gcatgccctc accgacatct
     3601 cacggaacca tgcctgatcg cgatggatct cctgatcatc accggaatcg tcctcgcact
     3661 catgctcaac ttcgtgaatg gcgtaaacga tgcatcccat tcgatcgcca ccgtcgttga
     3721 aacgagggcg ctctctcccc tgaaggcaag cgtctcatca gcgatcgcca attttatcgg
     3781 cccgttcgtg ttcaccaccg ctgtcgccgc aacgatcggt accgcgctgg tggtcccggg
     3841 tgctatgacc ccggtgtctc tcgtggtagc gatgctggtc tcgatcatgc tcatccttgc
     3901 tgccacccat atcgggctgc ctgtttcaag cagccatgcc ctcattggcg ggatactggg
     3961 gacaggggtg gctgttgctg gtctttctgc tcttattctg ccgccggcag caatgatcag
     4021 ggccctcgtc ctcttctctc tcactggcgc gggaatcgga gccctgatcc tcagtatcct
     4081 ggcatattat cttgaggaag ggatcagacc cgccctcgtg cttggggtga tattcggcat
     4141 ctcggtcacc atccccctca tgatccttgc gggtcttctt gttctttccg ggatcctcgc
     4201 cattatcgtc ttcattttta tctctcccgt gatcgggttc atcgctgcat ttctcttcga
     4261 tctcctggtc tcccacctgt ttcgcttctc ccggcagaat cggatgagac ggatctttca
     4321 gccgctgcag gtcgccgcaa gcctgttcca ggcagccggg catggtgcca acgatgggca
     4381 gcatgctgtc ggggttattt gtgccctgct ccttgccgga ggactgatcc cgttgttcat
     4441 ggtccccctg tg
//
LOCUS       DAOOCO010000155         4378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_18858_length_4378_cov_4.717095, whole genome shotgun sequence.
ACCESSION   DAOOCO010000155 DAOOCO010000000
VERSION     DAOOCO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18858_length_4378_cov_4.717095"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..539)
                     /locus_tag="PK154_08460"
     CDS             complement(147..539)
                     /locus_tag="PK154_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPW11127.1"
                     /translation="MDDAVRFFEEDSYARQAGIELIDVSPGRARVKMEIREQHLNSHR
                     TVHGGAIFTLADTAFALASNSHGIPAAAINAHISFLTAARSGNLYAEAEEFSRNPKLA
                     SYTVHVTGEDGQRIAIFQGMVFRKTPHP"
     gene            complement(746..1075)
                     /locus_tag="PK154_08465"
     CDS             complement(746..1075)
                     /locus_tag="PK154_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW11128.1"
                     /translation="MVQISQNAEYFLRRASEASHAADYLHALEYYDRALATDPHYADA
                     WHEKGNCLDELGRCDEAIASYDEAIKHDPYNAETWFNKGLILKRMGRESEAFRCINHG
                     IDLALGR"
     gene            1347..3998
                     /locus_tag="PK154_08470"
     CDS             1347..3998
                     /locus_tag="PK154_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW11129.1"
                     /translation="MNNHAEQRIDKIRNLLKGNPRGMSISEIAERLGLKRNIVSSDLN
                     YLERLGHVEMQSIGTSKVYFSSTKIPLAGILNYSSDMILILDTNGRVIEANVPLLKMI
                     GKTRDEIVGRNLEECPGPFFPAIQVGDINEGPIQNLTVWMPQLDDPRGVRHFRVKKIP
                     AVFEDAGQGTILLIGDVTEEIRYRDALQLSEAQYKAIVEDLTDIIFRFLPNGTITYVN
                     RTFRELFGQDGTEFRGANIFSYFSDPARLEFLEQVHETKGESSITTKVHEISTLNGAM
                     KFSLTIRALYNENMKIAEYQGIGRDVSPVQDAYDQMVLHSAEKEFLSRKSQIFLQTTN
                     EKEIFSHLASGIAEIVPDAVIILCSYNDSNKMMTIREIRDEKGVDLLEVLFEGNGIEF
                     SPSPEILKKTGEYDRIQNGQLAVIPTDLLYQLIGEVALNRIIQAIGHRMVYATLIVWD
                     EHMIGAAAVCLPEEGALRDRDLVETYIGIAALAFQRHIITESLSMSKNRFSIITETSP
                     LPISIISPEGEYLFLNSKFISKFGYTLADIPDGKRWFLHAFPDAKEMQKARDLWLSDL
                     KHSKKGEIRSRQFSVRCKDGKFRTIVFHPVTLLDGCQLVIYEDISDIEEAERVRNLLA
                     EIVRSSHDAIIGMTITGRIQTWNPGAVRIYGYTADEVVGKDIDIIFPTPLLNEKDWIL
                     ARVKAGEFISDFETRRVRKDGKVIDVSVTVSPIFDRDNTIIGTSTIVKDISSKKAEER
                     FRALESKYKDLVDSINVGVYRSTGDPEGKFIWGNSHLVHILGYSSFEAVQEIPISDMF
                     LKTNGRKELIDELKKNGFVKNREMMLKKSDGSVIYTRITALATFSSAGEISYINGIVE
                     DITENRILSRKLASLQGFSEENPPANP"
     gene            complement(4130..>4378)
                     /locus_tag="PK154_08475"
     CDS             complement(4130..>4378)
                     /locus_tag="PK154_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11130.1"
                     /translation="IEGYDPFRGISRKGEYPKFRGLDSSNLAGIKDRPEVKRHQYYSY
                     IVTAEKTENGIILKDPLRTEGIVFYPPSFTEITVPTGS"
BASE COUNT         1196 a          996 c         1031 g         1155 t
ORIGIN      
        1 taagcgtacg tcccggttta gcttatcaga tttacctatt aaatagtcta gggatccggg
       61 catgttacgg gtaaaccggt ggtgtggggg aatatctcaa taaagcagaa atgcgttctg
      121 ttcattggaa ggaactcact caaccatcag gggtggggag ttttccggaa taccatgccc
      181 tggaatatcg caatcctctg tccatcttct cccgtgacat ggacggtgta gctggccagt
      241 ttgggatttc tggaaaattc ctctgcctca gcatacaggt tcccggaccg tgctgcagtc
      301 aggaacgaga tatgagcatt aattgccgcg gcagggattc cgtgagaatt ggaagccagt
      361 gcaaaggccg tatctgcaag cgtgaaaatt gcccccccgt ggactgtgcg atgactattc
      421 agatgctgtt ctcggatctc catttttacc cttgctcttc ccggggatac gtcaatcagc
      481 tcgatgcccg cctgccgggc ataggaatcc tcttcaaaga aacggacggc atcatccatg
      541 cattatgttt cgatctcacc caagaaaagc gattggatgg gaatgatgta ttactctgtt
      601 tgatatctcc gtctcatctg aatataattc cgtgttggca gctgatgtcc atatggagaa
      661 gtaaagcctc ttaagcggat taaatgtcct gcggctcttc gaaataatga gagaatgaat
      721 atgaattacc actacgaatc agtattcatc ttcccagcgc aagatcgata ccatgattaa
      781 tgcaccggaa tgcttcactc tccctgccca tcctcttcag gattaatcct ttgttaaacc
      841 aggtctctgc attgtaagga tcgtgcttga ttgcctcatc ataactggct atcgcctcat
      901 cacaacgtcc aagttcatca agacagtttc ccttctcatg ccaggcgtct gcgtaatggg
      961 gatctgtcgc aagagcacgg tcataatatt cgagcgcatg cagataatct gctgcatgtg
     1021 atgcttcact cgccctgcgg agaaaatact cggcattctg tgatatctgc accacggatc
     1081 tccctgactg gttcaaactc attcctatca tcccctttgg aggcaatacc acgggttttg
     1141 atcgctccat tggcacaaaa gggtatttgg ttaatttaaa tatttttttg caatataatt
     1201 atgcacgtat aaattgacat gcactaggta gtccaccatt gcatggcatt accggcggtc
     1261 aatccctttt cctggaatgc cgggagagag atgcataggt actgcatgtg caaacctttt
     1321 tgagtcatac tcctgattac catccaatga acaaccatgc cgaacaacgg attgataaaa
     1381 tccggaatct tctgaaaggg aatccccgcg ggatgagtat atcggagatt gctgaacggc
     1441 ttggtctgaa gagaaatatc gtttccagtg atctgaacta tctcgaaagg cttggccatg
     1501 ttgagatgca atcgatagga accagtaagg tgtatttttc ttcaacaaaa atcccacttg
     1561 ccggcattct caattattct tctgatatga tcctgatcct tgatacaaac gggagagtca
     1621 ttgaggcaaa tgtccccctt ctcaagatga tcggcaaaac acgggatgag atcgtgggga
     1681 gaaatctcga agaatgcccc ggccccttct ttcctgcaat acaggtgggg gatatcaatg
     1741 aaggaccgat acagaatctc actgtctgga tgccccaact cgacgatccc agaggcgttc
     1801 gccattttcg ggtaaaaaag atccccgcgg tttttgagga tgcaggccag gggacaattt
     1861 tattgatcgg agatgtcact gaagagatcc gatatcggga tgcccttcag ctgagtgagg
     1921 ctcagtacaa ggcaattgtt gaggatctca ctgatataat cttccgattt ctcccaaacg
     1981 gaacaattac ctatgtcaac cgcacgttca gggaattatt tggtcaggac ggaaccgagt
     2041 tccgtggcgc aaatatattc tcttatttct cggatccggc gcgtctcgaa tttctggaac
     2101 aggtgcatga aacgaagggg gaatcttcta tcacaacaaa agtccatgaa atttcgaccc
     2161 tgaacggcgc tatgaaattt tctctcacaa taagggcatt gtacaacgaa aatatgaaaa
     2221 tagccgaata tcaggggatt ggtagagacg tatcgccagt gcaggacgcg tacgatcaga
     2281 tggtgcttca ctcggcggaa aaagaatttt tatccaggaa atcgcagatt tttcttcaaa
     2341 caaccaacga aaaagagatc ttttcccatc ttgcatcggg cattgcggaa atcgtccctg
     2401 atgcggtcat catactttgt tcttacaatg attcgaacaa aatgatgact atccgggaga
     2461 tacgtgatga gaaaggggtg gatctcctgg aggttctttt tgaaggaaac gggatcgaat
     2521 tttctccatc ccccgaaata ctcaaaaaaa ctggagaata cgacagaata cagaatggcc
     2581 agctcgctgt aatacccaca gaccttctct accagctcat tggcgaggta gcactcaacc
     2641 gcattatcca ggctatcggt catcggatgg tgtacgctac cctcattgta tgggatgaac
     2701 atatgattgg ggctgccgca gtgtgccttc ccgaagaagg agcattaaga gacagggatc
     2761 tggttgaaac ctatattgga attgccgcac ttgcctttca gcgacatatc atcactgaat
     2821 cactctcgat gagtaaaaat cgtttctcta ttattaccga gacctccccg cttcctattt
     2881 ccattatcag tccggaaggg gaatacctgt ttttaaatag taaattcatc tcgaagtttg
     2941 gatataccct tgcggacatt cccgacggca agagatggtt cctccatgcc ttcccggatg
     3001 cgaaggagat gcagaaggca cgggatttgt ggctgagtga tctgaaacat tcgaagaagg
     3061 gagagatacg atcgcggcag ttcagcgtaa gatgcaagga tgggaagttc cggacgatcg
     3121 tgtttcatcc ggtgacactt ctggatggct gccagctggt catctatgaa gatatttctg
     3181 atatcgaaga ggcagaacga gtacggaacc tcctggctga gatcgtcaga tcttcccacg
     3241 atgcaatcat aggaatgacg attacaggaa gaattcagac ctggaatccc ggtgcggtga
     3301 gaatttatgg gtatactgct gatgaggtcg ttggaaaaga tatcgatatc atatttccca
     3361 cccctctgct gaatgaaaaa gactggattc ttgccagggt gaaagcaggt gaatttatat
     3421 cggacttcga gacgagaagg gtgagaaaag acggaaaggt catcgatgta tcagttaccg
     3481 tttcacccat ctttgaccgg gataatacca ttatcggaac ttcaaccatc gtgaaagata
     3541 tttcatccaa aaaagctgaa gagcggttca gggctcttga atcgaaatac aaggacttgg
     3601 ttgattcgat caatgtcgga gtgtatcgca gtactggaga tccggaagga aaatttatct
     3661 ggggaaattc acatctggta catatcctcg gttattcttc atttgaagca gtgcaagaga
     3721 taccaatttc cgatatgttt ctcaagacga atgggcgaaa agagcttatc gatgaactga
     3781 agaaaaatgg ttttgtaaag aacagggaga tgatgcttaa aaaaagcgac ggatccgtta
     3841 tttacacccg gatcactgcc ctggcaacgt tcagttcggc cggcgagatc tcctatatta
     3901 atggtattgt tgaagatatc actgagaaca ggattctgtc ccgaaaactt gcctccctgc
     3961 agggattttc agaggagaat ccacctgcaa acccctgatc ctccccggaa ggcgtaatgc
     4021 acgagggtaa cctgttaaac cactgcgagg tcctttgata gtaatccccc catggtagtg
     4081 caggattccc tgcagcccgg agttgaaaaa tatcaggttt tgcctctcct cacgatccgg
     4141 ttggaacggt aatttcagta aacgaaggag gatagaatac aatcccttcc gtcctcagag
     4201 gatccttcag tataatccca ttttcggtct tttcagccgt aacgatatag gaatagtatt
     4261 ggtgcctctt cacttcaggc cgatctttta tacccgccag attgctggaa tccagacctc
     4321 tgaattttgg atattcaccc tttctggaga tgcccctgaa aggatcatat ccctcaat
//
LOCUS       DAOOCO010000156         4378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_18861_length_4378_cov_4.165626, whole genome shotgun sequence.
ACCESSION   DAOOCO010000156 DAOOCO010000000
VERSION     DAOOCO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18861_length_4378_cov_4.165626"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK154_08480"
     CDS             <1..302
                     /locus_tag="PK154_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW11131.1"
                     /translation="TTFEVEAGRTIVLRTFILTQGDGPVADSGTIWIGSVPTRATIYL
                     DGINRGQTDQYLSNVPAGTRNLTLVKAGYQNLTTTVDVKAGKTTVMKAFRLTIKP"
     gene            complement(292..450)
                     /locus_tag="PK154_08485"
     CDS             complement(292..450)
                     /locus_tag="PK154_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11132.1"
                     /translation="MPVIKGRKAGGGEEKGTSRSRCAGSPGRIQGRAGGDRNPRHQAA
                     PAGALIMA"
     gene            complement(695..2335)
                     /locus_tag="PK154_08490"
     CDS             complement(695..2335)
                     /locus_tag="PK154_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11133.1"
                     /translation="MKAKRLVKAAVAALLLCLLLGLPAGADIPVELDQFEPHFVQLGE
                     AGFDDPQNNYAWCMAEFKDDLYVGTGRNVPYTVFLQLKAAGMIPWDLTVSEITHPGGM
                     APPPFGPDPDPDTPGNQYEPDPSEVEHWAEDMSAEIWRYHDGSWEQVHTAQTFVNPYN
                     GYTYPEGVGYRYMITYTDLYGEEALYTGVGLGFGSTLLLKSTDGETWKPVVFGGAIPP
                     YDTRAMAVHDGRLYVGLGNGGIYATDDPSVDTDTWKKVADFGDNVAIGAIESYNGCLY
                     ATTWESIYNGGEGFEVWRSTTTNPGPGDWVRIVCGGAGDTWNPFGADIETFGGDVYVG
                     SMGLPFTSADGSAGLRSPKGFDLIRINDHGRWDLVIGSYVIPEDRQTATTEMRGMPMS
                     GWPSGFGNPLNLYCWQLEEHDCLFYLTTFDASSLLRVLDPELLGSIGFLDLLEPSLNE
                     VDVARMMPAGDMGTLFRMIREDNPFALVHALSAFLGGSDMWVSSDGVHWYPADLNGFG
                     DANNYGFRTLVSSDAGLYVGTANPFEGCQVYVGEVRAH"
     gene            3344..4357
                     /locus_tag="PK154_08495"
     CDS             3344..4357
                     /locus_tag="PK154_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11134.1"
                     /translation="MTTKSWVDLLVITAVILVIGTLVVSAADPVPASSGTSSLLLIIS
                     ADASGNLLSDSELQLTQGTTNLSDNPPLDLQGEGQAAVGYDEMTLATSGVISYTSTID
                     LDTSSQGTTGANLETARQIDYTNEGDGDGVGTMQSRESVLIEVDTAEGEPGEQGESAG
                     ACPAEESGEPCPTCSAAVSDAGSPASQTDSPYCVFSDPVDDDDEPLDDDTEIQDDDDE
                     DDSENGDESPASSETTTASSVRVIAGSEVQLKEGSVNSASSSRTIADNPDAGIAVSYL
                     VNVDGSGHSGDDRAEGAAEVYTDAFVMEGYGTNETTELSYYESVMAQGLIEISMETGY
                     SSP"
BASE COUNT          967 a         1207 c         1236 g          968 t
ORIGIN      
        1 cgacgacctt cgaagtggag gcgggcagga cgatcgtgct ccggacgttc atccttaccc
       61 agggcgatgg accggttgcc gattcaggga caatatggat cgggtcggtg ccgacgaggg
      121 ctaccatcta cctcgatggt atcaatcgcg ggcagaccga ccagtatctc tccaatgtcc
      181 ccgccgggac acggaacctg acgctggtga aggccgggta ccagaacctc accacgaccg
      241 tggacgtgaa ggcaggaaag accacggtca tgaaggcatt ccggctcacg atcaagccat
      301 gatcagggca cctgcaggtg cagcctgatg gcggggattg cgatctcccc ccgccctgcc
      361 ctgaatcctt ccaggactgc cggcacaccg gctccgggaa gtccccttct cttctcctcc
      421 tccggccttc cttcccttga taaccggcat ttccggtttt ttccttcgca atgatatccg
      481 tggttctgag acctggtgaa tgaggctgtt tgcaggaaat ggggtatagt tattgttctc
      541 atttcaggag gggactaacc caacggcctg gaatgagtct gggatcaatt tcctggacct
      601 cgcttccagt tcatcaggta tcgggagcca aaaaggtcag gccaatggga agaggatcca
      661 ggaagagttg ttcagaaaaa aaacgagggg agtgttaatg tgccctcact tcccccacat
      721 agacctggca tccttcgaag ggatttgccg tgccgacgta caggccggca tcactggaaa
      781 cgagcgtgcg gaagccatag ttgttcgcgt caccaaatcc gttcaggtcg gcaggatacc
      841 agtgcacccc gtcactgctc acccacatat cagagccgcc caggaacgcg gaaagggcat
      901 ggaccagggc aaacgggtta tcctccctga tcatgcggaa cagggtgccc atgtcgccgg
      961 ccggcatcat ccgggcgacg tcgacctcgt tgagggaggg ttcaagaagg tccaggaatc
     1021 cgatcgatcc gagcagctcc ggatccagga ctctgaggag gctgctcgca tcgaacgtgg
     1081 tgaggtagaa caggcagtcg tgctcctcca gctgccagca gtagaggttc agcgggttcc
     1141 caaacccgga cggccagccg ctcatcggca tgccccgcat ctcggttgtc gccgtctgcc
     1201 gatcttcagg tatcacgtaa ctgccgataa cgaggtccca ccggccgtga tcgttgatcc
     1261 ggatcaggtc aaatcctttg ggagaacgta acccagctga cccatcagca gaggtaaagg
     1321 gaagacccat cgaccctacg tagacgtcgc ccccaaaggt ctcaatgtca gcaccgaacg
     1381 ggttccaggt atcccctgct cccccgcaga cgatcctcac ccagtcgcca gggccgggat
     1441 tggtggtggt gcttctccag acttcaaatc cttcaccacc gttatagata ctctcccagg
     1501 tggttgcgta caggcagccg ttgtagctct cgatcgcacc gattgcaaca ttgtccccga
     1561 agtcggccac cttcttccag gtatcggtat cgacgcttgg atcatcggtt gcgtagatac
     1621 ccccattccc cagtcccaca tagagtctgc cgtcgtggac ggccatggcc ctcgtatcgt
     1681 agggagggat cgccccacca aaaacgactg gcttccaggt ttcgccatca gtggacttga
     1741 ggaggagggt cgatccaaat ccaagcccga ccccggtata gagggcctct tctccgtaca
     1801 ggtcggtgta cgtgatcatg taccggtagc cgacgccttc aggatacgtg tatccgttat
     1861 acgggttcac aaacgtctgg gcggtgtgca cctgctccca gctgccatca tggtagcgcc
     1921 agatctctgc ggacatatcc tctgcccagt gctccacctc tgatgggtca ggttcatact
     1981 ggttgccggg ggtatccgga tccgggtccg ggccgaatgg cggcggggcc atgccaccgg
     2041 ggtgggtaat ctctgagacg gtcaggtccc atgggatcat tcccgctgct ttcagctgta
     2101 aaaagacggt gtaggggaca ttccgcccgg ttcccacata caggtcatcc ttgaattcgg
     2161 ccatgcacca ggcataattg ttctgcgggt cgtcaaaacc ggcctcgcca agctgtacaa
     2221 aatgaggttc gaactgatcc agttcaacgg gaatgtcggc accggccggc agtcccagga
     2281 gaagacagag cagcaaagct gctaccgctg ccttcacgag tcttttcgcc ttcatatctg
     2341 ccaccttgaa tttaaaaaat ttacgattga aaattcaaag gtgtttgtat ataaatatat
     2401 tcccgatccg caggcccatt ccctctcatt cagggaggat ttctggaaaa aaatcgtttc
     2461 ctctcctttg gagcgtcccc ctgttgatcc gggagggaag tcatcttctg ctctttattg
     2521 cgacgaaagc accccccggc aggtctccgt ttccagcccg ggacctctca ctccgtttcc
     2581 agtctgggac ctctcactcc gtttccagtc tgggaccgct gaatcatagc ctgggatctc
     2641 tgaagtgcct gctctctttg ggcagagatg aaaatggagg gggagtgggt gggggaatgg
     2701 tggtatctgc aggggatgat cagccgtttt ccccgggagc cttgcccggg aggcaattcc
     2761 agggatcagt atggtcaaaa aaagcccgtt ttgggctccg tttccctggt tctgccgaat
     2821 gggatccgca tgttgccggg aacagcgtct cggttctctc aaatgtcctt ttaaatcggc
     2881 ttttttcaaa aataacgatt ataatcgctt taaaaaaaga ggccgtatct ggtttccagc
     2941 aggcagggat tcctgggggt atattccatt gatggaacca aatgatcggt gctgtatgca
     3001 atctgtgaag ggttttcttc ggcctggttt tcaggtcgtt tttattggcc aagttatatt
     3061 taaacgtgtt attttagcga taaccgggtc aatagccggg caggagcccg aataccctct
     3121 gaaaaagcct gccagtccct gtgctcctct cccgtcatca tgatcggaat gataggatac
     3181 tgcctgatcg atcaggggat atgaccgggg cgattccgct tacctttata taccaggtta
     3241 ctgttattgt aaatattcat tattgtgata aacattaatc actgtgatga atccctggac
     3301 accgagcacg ggtgtcgagg aagaaaacat gcaaggtggc gaaatgacga cgaaatcatg
     3361 ggtggatctc ctagtgataa ccgcggtgat cctggtgatc ggcactctgg tggtgagtgc
     3421 agcagatcct gtacccgcaa gcagcggtac atcatctctc ttgttgatca tctctgcaga
     3481 tgcgagcggc aatctccttt ctgacagcga actccaattg acccagggga cgacgaacct
     3541 gtccgacaac cccccgctcg atctccaggg ggaggggcag gctgcggttg ggtatgatga
     3601 aatgaccctt gcgaccagcg gggtgatcag ctataccagt acgatcgatc tcgatacctc
     3661 cagccagggc accacagggg ccaacctcga gaccgccagg cagatcgact atacgaatga
     3721 aggagatggg gatggtgtcg ggaccatgca gtccagggaa tcggtgctga tcgaggtcga
     3781 taccgcagaa ggtgaaccag gtgaacaagg tgaatctgcc ggggcatgcc ccgctgaaga
     3841 aagtggagag ccttgcccga cctgttcagc ggcggtctct gatgctggct cgccagcgag
     3901 ccagaccgat agcccgtact gtgtgttttc tgaccctgtt gatgacgatg acgaaccgct
     3961 ggatgatgat accgaaatcc aggatgacga tgatgaggat gattcggaga acggcgatga
     4021 gagtcccgcc tcgtcagaga ctaccaccgc ctcttctgtc cgggtgatag cagggagcga
     4081 agtccagctc aaggagggtt cggtgaactc cgcaagttca tcacgaacca tcgcagacaa
     4141 ccctgatgca gggatcgcgg tatcctatct cgtgaatgtc gatggaagcg ggcattccgg
     4201 cgatgaccgg gcagaggggg ccgccgaggt ctataccgat gcattcgtga tggagggata
     4261 cgggaccaac gagacgacag agctgagcta ttacgagagt gtcatggcac agggtcttat
     4321 cgagatctcg atggagaccg gctattcttc cccgtgaaga gatcccccgc cctttttt
//
LOCUS       DAOOCO010000157         4280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_19470_length_4280_cov_5.050414, whole genome shotgun sequence.
ACCESSION   DAOOCO010000157 DAOOCO010000000
VERSION     DAOOCO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19470_length_4280_cov_5.050414"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK154_08500"
     CDS             complement(<1..286)
                     /locus_tag="PK154_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HPW11135.1"
                     /translation="MSLFLTTVPVEEAVNTARSLAIRLSSEEIPLNAAFGRVLSNEIT
                     ADIDIPGFSRSSVDGYAVRFRDTTGASESLPALFSLSGRAAMGAEGVKTLT"
     gene            complement(283..459)
                     /locus_tag="PK154_08505"
     CDS             complement(283..459)
                     /locus_tag="PK154_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11136.1"
                     /translation="MDEEKIRSILEREGIDKEIKCPEAFAISEKYGVSKTDIARFCNT
                     HGVKIRACQLGCFK"
     gene            647..1846
                     /locus_tag="PK154_08510"
     CDS             647..1846
                     /locus_tag="PK154_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPW11137.1"
                     /translation="MIIHESDSRLIYLDHLDKELDCDLIIFLSRHSSEHPRPVLTVHV
                     TGNIADAGLGGFAKSLAVASPEWMHAVLGNLEKNAPEGYHITYEVTHHGPTELATPSF
                     FVEIGSTEKEWTDLRAGYAVALSVLEAYPHETINVLGIGGSHYARRETEIALQSRTAF
                     GHIVHSRYVPCLDPSMIAGLVEKSQAKAVYIDKKAVNPSGMHHLTIVLANLGIPRLSE
                     RQLLQLRDISWFTWEEIQRVAQEVSPGAWIDISPHVKDGIPNVVRLPEDLFYETIRID
                     PTGLKEGAGNLPVAILSSKGSAVLPIFITTEENTERILHDLISLCVTLICSGETTAVD
                     GDQLIISRTRFDPEQARILGIPKGPLYGELLKGRTVRINGREITSDMVQTRTTTCIRI
                     PGLERLI"
     gene            1843..2934
                     /gene="ftsZ"
                     /locus_tag="PK154_08515"
     CDS             1843..2934
                     /gene="ftsZ"
                     /locus_tag="PK154_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW11138.1"
                     /translation="MRSIVEEALTKVSTEPAKVLSENEELDQILKELKTEITVIGCGG
                     SGSNTITRMKEEGIHGANLIAMNTDAQHLARTLADHRILIGRQRTRGLGAGSIPQIGE
                     EAALENEQDIKKIVSGSDMVFITVGLGGGTGTGAAPIVAKAARDEGALTIAVVTLPFT
                     AEGAIRMENAEAGLERLRDVADTVIVVPNDRLLEVVPRLPLYAAFKVADEVLMRAVKG
                     ITELITMPGLVNLDFADVRTVMERGGVAMIGVGESDSEDKAADSVKKAIRSPLLDVDI
                     SGATAALVNVIGGPDMTMSEAEGVIQEVYDRIDPGARIIWGAQVDPSMQSKMRTMLVV
                     TGVRSPQIYGRNEKITPKVQKQFEIDFLK"
     gene            2941..3135
                     /locus_tag="PK154_08520"
     CDS             2941..3135
                     /locus_tag="PK154_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HPW11139.1"
                     /translation="MEIAKPDIKFDVNEELFRKYWRILKLARTPTKEEFRKIALVAAA
                     GVLIVGLIGFLIYIGMIPLS"
     gene            3141..3614
                     /locus_tag="PK154_08525"
     CDS             3141..3614
                     /locus_tag="PK154_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HPW11140.1"
                     /translation="MEEYTNRIFAIKTTSKQERTVADNIRKAIDTGATDVKVTSIIVP
                     DELKGYVLVETPESLARIEQLAEKVAHARTVVRGETTFEEVAHFLVPKPVVSGISEGT
                     IVELIAGPFKGEKAVVKRIDAGKEEITVELYESVLPIPITVRGDNVRVVDRGESA"
     gene            3714..4190
                     /locus_tag="PK154_08530"
     CDS             3714..4190
                     /locus_tag="PK154_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPW11141.1"
                     /translation="MADVVEVLVSGGKATAGPPIGPALGPLGINVKAVVDEINKKTSE
                     FNGMQVPVKIEVDDKKNFTVTVGIPPTTALIKKEVHIEKGSPEPNLKVVGDLPMQAAV
                     RIARMKAGDMLSYDLKNAVKEVVGTCVSMGVNVEGKRPKEILAAIDAGTYDSVLAQ"
BASE COUNT         1099 a         1036 c         1183 g          962 t
ORIGIN      
        1 aggtgagtgt ttttactcct tccgctccca ttgcagctct cccggagaga ctgaacaggg
       61 ccggcaggga ttccgacgcc cccgtggtgt cccggaatct gacagcgtat ccatcgaccg
      121 atgatcggga gaatcctggg atatcaatat ctgccgtgat ctcgttagaa agaacgcggc
      181 cgaatgcagc attgagcggg atctcttccg aggacaggcg gatagcgagg ctccgtgcgg
      241 tgttgactgc ttcttcaact ggtactgttg tcagaaaaag gctcatttga agcagcccag
      301 ctggcaggcc cggattttta ctccgtgagt gttgcagaac cgtgcaatgt cggtcttgga
      361 gaccccgtat ttttcagaaa tagcaaatgc ttccgggcat ttaatctcct tgtcgatccc
      421 ctctctctcg aggattgacc gtattttctc ttcgtccata gtaacaatag tatgtgtcca
      481 aagaaatagg gtttaccatg tctgtctctt ataaaggagg tgtcttctga agatagcact
      541 cgtccattcc cgactcgatc ccgcgggaac gacaattcag tcatgcctgg aagatttact
      601 gacagaggct ggtcataagc gcccttatcc gcttgaagag catgatttga ttattcatga
      661 gtcagattcc cgcctgatct accttgacca tcttgacaaa gaacttgatt gtgatctcat
      721 catattcctc tccaggcatt catcagaaca tccccgccca gtcctgaccg tgcatgtcac
      781 cgggaatatc gcggatgccg gcctcggcgg gttcgccaag tcacttgctg ttgcttcacc
      841 ggaatggatg catgccgtgc tcggaaatct ggagaaaaat gccccggaag gataccacat
      901 cacctacgaa gttacccacc atggcccgac ggagctggcg acaccttcgt tctttgttga
      961 gatagggagt acggagaagg aatggaccga tctgagggct ggatatgcgg tagctctgag
     1021 tgtcctggag gcatatccgc acgagactat aaatgtatta gggattggcg gctctcatta
     1081 tgcacgcaga gagacagaga ttgccctcca gtccagaacg gcgttcggcc atatcgttca
     1141 cagcaggtat gttccgtgcc ttgatccttc aatgattgct ggcctcgtag agaaaagcca
     1201 ggctaaagcc gtgtatatcg ataaaaaagc cgtcaatcct tccggaatgc accatctcac
     1261 gattgtgcta gcaaatcttg gtattcctcg cctttcagaa cgtcagctcc tccagttacg
     1321 ggatatttcc tggtttacct gggaagagat ccagcgagtg gcacaggaag tatctcccgg
     1381 agcttggatt gatatctctc cccatgtaaa agacgggata ccgaacgttg tcagactccc
     1441 ggaggatctt ttttatgaaa caatacggat cgatcccact ggtctgaaag agggagcagg
     1501 aaatttgccg gttgctatcc tctcgtcaaa aggatctgca gtgttgccga tattcataac
     1561 aacggaggag aacacggagc gaatactaca tgatttaata agcttatgcg ttacactgat
     1621 atgtagtgga gaaaccacgg cggttgacgg ggatcagctg atcatcagca ggacacggtt
     1681 tgacccggaa caagcgcgca ttctgggcat cccgaagggc cccctgtacg gagaactgct
     1741 gaaaggcaga acggtgcgga taaacggccg tgaaatcaca tcggacatgg ttcaaaccag
     1801 gacaacaaca tgcatccgaa tcccgggatt ggagagactg atatgagatc gattgtcgaa
     1861 gaagctttga ccaaggtgag tactgagcct gcaaaagtcc tttctgaaaa tgaggaactt
     1921 gaccagattc taaaagagct caaaaccgag attacggtca tcggttgcgg agggagcggg
     1981 tcaaatacca tcacaaggat gaaggaggag ggtatccacg gggcaaacct gattgcaatg
     2041 aacaccgatg cccagcacct tgcaaggact cttgcggatc accggatcct tatcggaagg
     2101 caacgaacaa ggggccttgg tgcgggatcc atcccccaga taggagaaga agcagctctc
     2161 gagaatgaac aggatataaa gaaaattgta tcaggcagcg acatggtctt catcacggta
     2221 ggactagggg gtggcacggg aacgggcgct gccccgatcg ttgccaaggc ggcaagggat
     2281 gagggagcgc tcaccattgc ggtggtaacc cttcctttca cggctgaggg ggcgatccgg
     2341 atggaaaatg ctgaggcagg tcttgaacga ctgcgcgatg ttgccgatac cgttattgtc
     2401 gtgccgaatg accggcttct ggaagtcgtc ccacgcctcc ccctgtatgc cgctttcaaa
     2461 gttgccgatg aagtcctgat gcgtgcagtg aaagggatca cggaacttat caccatgccc
     2521 gggcttgtga accttgattt tgctgatgtc cgtactgtta tggaacgggg cggagtggcc
     2581 atgatcgggg tcggggagag tgacagcgag gataaagcag cagattctgt gaaaaaggcc
     2641 atccgctctc ccctccttga tgtcgatatc tcgggtgcca ccgctgcgct cgtgaacgtg
     2701 attggagggc ctgatatgac catgtcagag gctgaaggag tcatacagga agtgtacgac
     2761 cgtatcgatc cgggcgcgcg tattatctgg ggagcccagg ttgatccttc catgcagagc
     2821 aagatgagaa ccatgctcgt agtaaccggt gtacggtcgc cacaaatata tgggaggaac
     2881 gagaagataa cccccaaagt tcagaagcag ttcgaaatcg actttctgaa gtgatacccc
     2941 atggaaattg caaagcccga cataaaattt gatgtgaacg aggaactgtt caggaaatac
     3001 tggcggatcc tcaagttggc gagaaccccg acaaaagagg agttcaggaa gattgcactg
     3061 gttgcggcag ctggtgttct gatcgtcggt cttattggat ttctgatcta catcggcatg
     3121 atccctctgt cctgattgct atggaagagt acaccaaccg tatttttgcc atcaagacta
     3181 cgtccaagca ggaacgcact gtcgccgaca atatcaggaa agccattgat actggcgcga
     3241 ctgatgtgaa ggtcacctcc attatcgtcc ctgatgaatt aaagggatat gtacttgtcg
     3301 agactcctga aagtcttgca cggatcgaac agctggcaga gaaggtcgct catgcgcgca
     3361 ctgtggtgag gggcgagacg acatttgagg aggttgcgca cttcctcgta ccgaaaccgg
     3421 tagtcagcgg catatctgaa gggaccattg ttgagctcat cgctggtccc ttcaaaggtg
     3481 agaaggcagt cgtaaaacga atcgatgccg gcaaagaaga gatcacggtc gagctctacg
     3541 aaagtgttct gcccatcccc atcacggtca gaggggacaa tgtccgcgtg gtcgaccgcg
     3601 gggaaagtgc atagatcatc ccatcatctt aaggctataa cctttaagtc ttcacgagta
     3661 tacctttttt aacccacgct gaaaccgttt cagcgggaca gcatggtgat accatggcag
     3721 acgtggtcga ggtattagta tccggaggca aggcgaccgc aggtccgcca atcggacccg
     3781 cactcggacc actcggtatt aacgtgaagg cagttgtcga tgagatcaat aagaagacct
     3841 ctgaattcaa cggcatgcag gtgccggtga agatcgaagt cgacgacaag aagaacttca
     3901 cggtcaccgt gggaattccg cccacgacgg ctctcatcaa aaaagaggtt catatcgaga
     3961 aaggatcccc cgaacccaac ctgaaagtgg ttggcgactt gccgatgcag gccgcggtca
     4021 ggatcgcccg gatgaaagca ggcgacatgc tctcttatga tctcaagaat gcggtcaagg
     4081 aagttgtcgg aacctgtgtt tcgatgggtg tgaacgttga aggcaaacgc cccaaggaga
     4141 ttcttgcggc gattgatgcc ggaacgtatg actcggttct tgctcagtaa cgaattggct
     4201 tacaacccat agaagatcac aagtggtcga ggactatgga ggaaccatat ggtagaaagg
     4261 gccagaattt tggaggccgt
//
LOCUS       DAOOCO010000158         4209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_19913_length_4209_cov_4.251565, whole genome shotgun sequence.
ACCESSION   DAOOCO010000158 DAOOCO010000000
VERSION     DAOOCO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19913_length_4209_cov_4.251565"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1371)
                     /locus_tag="PK154_08535"
     CDS             complement(610..1371)
                     /locus_tag="PK154_08535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HPW11142.1"
                     /translation="MDPAVAISFTGTILLELGFPLLVAWFLIRRYHLSWTLFLYGALF
                     FILVQIVHTPLVLFTQAPLSEALNAAIPNRTFALAVFAVILGLLAGLFEEIGRYLVFS
                     RFFPSRNIATSREHGLLFRAGWGGVESMMIAGLLALTLVAYLFSASPVLASLTPLDPL
                     VSLLERLMVFTLQIAFTLLVLLSVAQARPLLLGLAILWHTGVDALAVFLGVTSGIAVT
                     EAMVAVNATLALIYLWYQRPERHREDEHQPQSAIR"
     gene            2179..2571
                     /locus_tag="PK154_08540"
     CDS             2179..2571
                     /locus_tag="PK154_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPW11143.1"
                     /translation="MKQRLAAWGSEYYLMRDSTISLSGESIMGEDTIFSIGEWTVTPG
                     KEALFLERWREFSRWSLNHINGGRWVYVVQDREQNNKFMSFGIWESPEDYTEWRHSTK
                     FKSAFAEFSAMCDKVAYSTSTEVLHRYR"
     gene            2645..3892
                     /locus_tag="PK154_08545"
     CDS             2645..3892
                     /locus_tag="PK154_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPW11144.1"
                     /translation="MPLPRRPLILCLLALVIGTLLVSGCLTEPPAAKPPVTMNMSSLT
                     LYTEDVPPYNYVDNGTLQGISVDLLSLITAGMGENLSWEQVKVVPWSEGYTAALTGNK
                     TMIFAIARLPERETSFKWAGPIIPLTPVLFALPERGIVVTSPDDLEGYRIGAVAENAE
                     VQQLLEIGVNQSRIVLAPNTSVMVTMLQDGEIDLMAYSQAAGRYFTRQVTGSFDTFRV
                     VYVFPDIPLYYAFSRDVPDETVLSFQKVLDTLKTEKEAEGFSEYERILYRYTGVSCQP
                     QPYPVAEVMNLVNVTVAAIEKDAPGTIRRVNAGEAPYRDPANSALYAFVFDRNVTLVA
                     QAVNPGQVGTNLYGKTDVTGTPFRDRIVTGALHNGSGWEEYIYMNPTDAGLYYKTSYY
                     RVTEGSDGMTYIVGSGAPSACEW"
BASE COUNT          973 a         1049 c         1217 g          970 t
ORIGIN      
        1 cctgtttctg gtaccctgcg atctcgctct ccagttcctt tattttctgt ttgacgtatg
       61 tcatacggtc acctccgttc tggtccgttt tccggctcgc cgccggatca tcccctcggg
      121 ataccggtta cctttttaag gaggacaggg agtccgggtg gagttctccc ggttgatcgc
      181 ggggtatctt gcttctctgg acttccttgc ctcacgagag tggacaacct ccgaaagggg
      241 actgctctcc ttctcgatac gccaccaggg tatggtggat gccaatacat gccgataaaa
      301 gacggatcta gcgagggagt ggatccagtg atatccaggg attgccgggc gactgcccgt
      361 ggctccgtcg ttctgcccgg tggaatgggt cccccatctg ttctccaatc aggggaacta
      421 tggatatcgg tctcggtatc gatctgtctt tattggcgtg acattcctgc tggcccctat
      481 tgcttccagc acgaacgttg tattgtgatc catagatagt cctgcagatt gaaatacttt
      541 gtgcagaata ttatcgtata cgcccctttt tttccatagg acgttagaga tccttttttt
      601 aagaacttgt cagcgtattg cggactgagg ctgatgctca tcttcgcggt gacgctcagg
      661 tctttggtac cagagataga tcaacgcgag agtggcgttg actgcgacca tcgcctcggt
      721 gaccgcgatc ccggaggtga ccccgaggaa cacggcaagg gcgtcgaccc cggtatgcca
      781 gaggattgcc aggccgagca ggagcggtct cgcctgggct acggagagga gcaccagcag
      841 ggtgaaggcg atctggaggg tgaagaccat cagccgctca aggagggaga ccaggggatc
      901 gagaggggtg aggctcgcga gcaccgggga tgcggagaag agatacgcga cgagtgtcag
      961 ggcgaggagc cctgcgatca tcatgctctc gactcctccc cagccggccc tgaacaggag
     1021 gccatgctcc cgggaggtgg cgatgttccg ggacgggaag aaccgggaga agacgaggta
     1081 gcggccgatc tcttcaaaga gaccggcgag gagaccgagg atgaccgcaa ataccgcaag
     1141 agcgaatgtc cggttgggga tcgctgcatt gagcgcctca gagagcggag cctgggtgaa
     1201 gaggacgagc ggggtatgca cgatttggac caggatgaag aagagtgctc catacaaaaa
     1261 gagggtccag gagaggtgat atcgccggat gagaaaccag gcgaccagga gggggaatcc
     1321 gagctcgagg aggatggttc cggtaaaaga gatagccacg gcgggatcca taggggttca
     1381 tcgatggatg gcgattaaaa tctttccccg gaggggcagg atgagttgct ggaatggatt
     1441 ccacagagta ttgacatttt ttggtctgca cagtgaaaag gtaaaacctg ctgaatcagg
     1501 ctgccagaaa aggaaaaagt ctcttattta tgccgttctt tgtgataatt cggtcgtata
     1561 tgctgatcaa tgagtcaatg ttcctcggca gatcagaccc ccctaccttc atcgaccgga
     1621 ttatacctgg taattgttca tatgacttca ttgctgtaca ccccctttct tttattctct
     1681 ccccagcacc attcatcctg ctcgggacca attgacagat aagttcctgt tataatattt
     1741 ttttgccatg cacatcttat tccagaacat tattgagtct cttttcaaaa tcagagcgat
     1801 ttgatcgctt tgccatgcaa tgggtgatgc agaacagaca tggggagggt gatcatgatt
     1861 ccccactcga aaccattgcc atgcagatat gaaaaaacat aggatcaggt ggtgtacaga
     1921 atttccttgc acattcaccc tgattggtga tcctatgtga tctgatctgg gagggtaagt
     1981 gaagtcagaa caagaagcgg gaactctttc cagaagcagg acccccgctg tcggccattg
     2041 cagggggagc cggtctggga gatattgggg aacgggcagc atcatcccca gagcggttct
     2101 tgtttcagga aacggattgg caggataccg ttcctctgct ggtatgaatc acgtccacac
     2161 tctaatggtt ttcatggaat gaagcagcgt ctcgctgcct gggggagcga atactattta
     2221 atgcgggata gtactatcag tctttcaggg gaatcgatca tgggcgaaga tacgatattc
     2281 tcaatcggtg aatggacggt cactcccggc aaggaagcat tgttcctcga aagatggcgg
     2341 gagttctccc gctggtctct gaatcatatc aacgggggac ggtgggtgta cgtggtccag
     2401 gaccgggaac agaataataa attcatgtca tttggtatct gggaatcccc ggaggattat
     2461 actgaatggc ggcattcgac aaaattcaaa tcagcgtttg ctgaattcag tgccatgtgt
     2521 gataaggtcg cttacagtac ctcgaccgag gtattgcatc gctaccggta agatcgtcct
     2581 tcctttttca ccatctgcag agaaatgcac aatgtttatc catataaccg ggagattcac
     2641 acgtatgcca ctcccccgcc gtccactcat cctctgcctt ctcgccctgg ttatcggaac
     2701 actactcgtc tccggatgcc tgactgaacc cccagctgca aagccacccg tcactatgaa
     2761 tatgagcagt cttactctct acaccgagga tgttccgccc tataactatg tggataatgg
     2821 aaccctgcag gggatctcag tcgatctcct ttcgttgatc accgcgggga tgggagagaa
     2881 cctgtcatgg gaacaggtga aggtagtacc ctggtcagag gggtacacag cagcccttac
     2941 cggaaacaag acgatgatct ttgccattgc ccggcttccc gagcgggaga cctcgttcaa
     3001 atgggcagga cctatcattc cactcacccc tgttctcttt gccctgccgg agcgcgggat
     3061 tgtcgtcacc agccccgatg acctcgaggg ataccggatc ggagccgtcg ctgaaaatgc
     3121 cgaggtccag caactgctgg agatcggggt gaaccagagc cggattgtgc tggcgcccaa
     3181 tacatcagtt atggtgacga tgctacagga tggcgagatc gatctcatgg cgtactcgca
     3241 ggctgccggc aggtacttca cccggcaggt gacgggaagt tttgacacct tccgggtcgt
     3301 gtatgtcttc ccggatattc ctctctacta tgcgttcagc cgagatgtcc cggatgagac
     3361 ggtgctgtcg ttccagaagg tgcttgacac actcaagacc gagaaggaag cagagggttt
     3421 cagcgaatac gagcggatac tgtaccggta cacaggagta agctgtcagc cacagcctta
     3481 tcccgtggca gaagtgatga atctggtcaa tgtgacggtg gcggcgatcg agaaggacgc
     3541 tcctggcacc atccggcggg tcaatgcagg ggaggccccc tatcgggatc cagcaaattc
     3601 tgctctctat gcatttgtct ttgaccgcaa cgtgaccctc gttgcccagg cagtcaaccc
     3661 aggacaggta gggaccaatc tctacggaaa gactgatgtg accggaacac cgttccgcga
     3721 taggatcgtc acgggagcac tccataatgg gtcagggtgg gaggagtata tctacatgaa
     3781 tcccaccgat gccggcctgt actacaagac gtcctattat cgggttaccg agggaagtga
     3841 cgggatgaca tacatagttg ggagcggggc tccttcggcc tgcgagtggt aggaagggac
     3901 aaccaaaaat gcttccctga ctctccgggg aggatctgcc ttacgatgaa cccagagacc
     3961 ggtttggttc gagggggtag tctgggtgaa atcgttactt caggatgctt gcaggacccc
     4021 ggctatgatg agtgaccccg aatcacctca tcacgggctc gatctccttc tggtatgatc
     4081 atcacgatgg tagggggaaa gtgagtgaaa gaaaaacggg aggtgattgt tataggccgt
     4141 ttctcggggg aaaaacaggt gcgtcgggcg gtgtcggctc gccgggaaat cccgggctgt
     4201 ctcccacac
//
LOCUS       DAOOCO010000159         4208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_19918_length_4208_cov_6.496268, whole genome shotgun sequence.
ACCESSION   DAOOCO010000159 DAOOCO010000000
VERSION     DAOOCO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19918_length_4208_cov_6.496268"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..507
                     /locus_tag="PK154_08550"
     CDS             76..507
                     /locus_tag="PK154_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HPW11145.1"
                     /translation="MTTVFDVPADRYIRSLAEELKKKPEITPPEWSLFAKTGVHKEMP
                     PEDPEWWFVRVASIMRRVYIDGPIGVERMRTFYGGGKDRGSRPTQFRKGSGSILRKSL
                     QQLEAAGLIIHDKTGRRVSPQGMSFMDNLARSLVQQAEKTA"
     gene            524..865
                     /locus_tag="PK154_08555"
     CDS             524..865
                     /locus_tag="PK154_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPW11146.1"
                     /translation="MGDDELAEIRRRRMAQMQQQAMDQQMMQEDMERQKQADAQLHMV
                     LLQILEPEARERLNAVRLARPEFARAVEQQLVLLAQSGRLKQKITDAQLKALLQQIQP
                     AKKEFNIRRKG"
     gene            862..1473
                     /locus_tag="PK154_08560"
     CDS             862..1473
                     /locus_tag="PK154_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HPW11147.1"
                     /translation="MKAGVLFSGGKDSSLAALLLSRDYEVELNTFIFHQEDDTAGVEN
                     AARAVGLPWRRRIFDEGFLERVIGMIVSCGYPNEGIQEVHRTAVELLCRDYPVVADGT
                     RMDDRIPMLTRSEVQSLQDRTGCSYLRPLLGYGKREVDRLARCHFVIVNGQTGQIENG
                     DYERRIRNGIAAEGLDPRAYFPEVHEQSLVLSRAVPGTEVKFR"
     gene            1470..1622
                     /locus_tag="PK154_08565"
     CDS             1470..1622
                     /locus_tag="PK154_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HPW11148.1"
                     /translation="MSKSTKGFKIRLAKACEQNRRVPQWVMLRTKRAVVSHPRRRNWR
                     RSALKV"
     gene            1627..1890
                     /locus_tag="PK154_08570"
     CDS             1627..1890
                     /locus_tag="PK154_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HPW11149.1"
                     /translation="MAEKTQEQIYIIPLRDARRAPRWKRSHTAIKDIRKYLEKHLKSH
                     DVKLDKSINERIWDRGSENPPSRVRVRAMKFDDGQVQAELAEE"
     gene            1892..2560
                     /locus_tag="PK154_08575"
     CDS             1892..2560
                     /locus_tag="PK154_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HPW11150.1"
                     /translation="MEKTVSFGGDCNIGVFTRVFDDIAVVPPNAPGDFCEALTGLLGV
                     EIVETTIQGSAIIGSLLAGNRHGFVVSGFAEETEIARLSEYRELCLLTHTMNAAGNIL
                     LASDSLTLAHPDMPEQMAEEIGDILKTPVHRTTIAGIKTVGMAAVASARGIMVHPRTT
                     RHELAGLESLSDLPIGTGSVNMGSGLVGTGLVANSRGYVAGLETTGFELGRIEDIFGF
                     LERT"
     gene            2560..2751
                     /gene="rpl18a"
                     /locus_tag="PK154_08580"
     CDS             2560..2751
                     /gene="rpl18a"
                     /locus_tag="PK154_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HPW11151.1"
                     /translation="MQNMKFEVTGTFMIGDEWHPYRKVIEAPNEVQAKERTFAVIGSK
                     HRLKRRYITVNAVTPVNGE"
     gene            2741..3217
                     /gene="pfdA"
                     /locus_tag="PK154_08585"
     CDS             2741..3217
                     /gene="pfdA"
                     /locus_tag="PK154_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HPW11152.1"
                     /translation="MVNNVDRVDPRELQSLQLYLNDYRQQAEIFTRQLALLEEGRMEA
                     LAAIEALEGAAAAPDSIVLLQIGGGVSVRARILDPDRVLLNIGADVIVERPNAAAQDY
                     LKDRITEMEASAKKVAETLERLRVQMNELARRIELGYQQAQMAQMQPPPVPEAGEE"
     gene            3236..>4208
                     /gene="ftsY"
                     /locus_tag="PK154_08590"
     CDS             3236..>4208
                     /gene="ftsY"
                     /locus_tag="PK154_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPW11153.1"
                     /translation="MFDGLRAKLQKIQGGLSQSIESASEKEAIPPTSSGGMEKNPTIL
                     GKVKVLLTDREFLVDEKDIREPLFELEMVLLENDVALPVTDEIISHIRKDLTGSRRKI
                     GESVDSIVTSALRGALLDVLGEGFDLVSYLSSHEKPVKILFTGVNGTGKTTTVAKVGS
                     FLKKNGFSVVIGAGDTFRAGALEQIDVHAQRLGIKIIQHQAGGDPSAVLYDTVQYAEA
                     HHIDAVLADTAGRFHNRVNLMNQLDKIRRVMKPDLIVYVDEAVAGNDAVVRAHEFKKA
                     VGADAVVLTKADMDPKGGAAISIAHTIGKPLMFLGIGQGYDDLVPFSP"
BASE COUNT         1009 a         1112 c         1278 g          809 t
ORIGIN      
        1 gaaatatata aaagtattat gaaccaatat gtaaagactg ttaaatcaat tcggtgacag
       61 tgaggttata agaggatgac aacggtattt gatgtgccgg ctgatcgcta cattcgctcc
      121 cttgcggagg aactgaagaa gaagccggag atcacccccc ccgaatggtc actttttgcc
      181 aagaccgggg ttcacaagga gatgcccccg gaggatccag aatggtggtt cgtccgggta
      241 gcatccatta tgcgccgggt ctacatagat ggaccgatag gcgttgagcg gatgagaaca
      301 ttctatggcg gaggcaagga ccgtggctcc aggccgacac agttccggaa aggaagcggc
      361 tcgatccttc gaaagtcact ccagcagctc gaggccgctg gactgatcat ccatgacaag
      421 accgggcgga gagtctcacc ccaggggatg tctttcatgg acaatcttgc ccgaagctta
      481 gtacaacagg cagagaagac tgcctgaatg cggggaatac accatgggag acgatgagct
      541 tgctgaaatc cgccggaggc ggatggcgca gatgcagcag caggcgatgg atcagcagat
      601 gatgcaggag gatatggagc ggcagaaaca ggctgatgcc cagctgcaca tggtccttct
      661 gcagatcctt gaacctgaag cacgggagcg gctcaatgcg gtccggctcg cccggccgga
      721 atttgcccgg gcggttgagc agcagctggt gcttcttgcc cagagcggtc gtctcaagca
      781 gaagatcact gatgcgcagt tgaaagcgct gctgcagcag atacagccgg caaagaagga
      841 attcaatatc cgaaggaaag gatgaaggca ggggtgctct tttccggtgg gaaagacagc
      901 tcccttgcag cgcttctgct ctcccgtgat tacgaggtcg agctgaacac cttcatcttc
      961 caccaggaag acgatactgc cggggtcgag aacgcggccc gggctgtcgg ccttccatgg
     1021 aggcggagga tatttgatga aggtttcctg gagcgggtta ttggaatgat cgtgtcatgc
     1081 gggtatccaa acgaggggat tcaggaggta caccggacgg cagtggaact gctgtgccgt
     1141 gactacccgg tcgttgccga tggtacacgc atggatgacc gcatccccat gctcacgcgc
     1201 agcgaggtgc agagcctcca ggacaggacc gggtgctcct acctgcgccc gctcctcgga
     1261 tatgggaaaa gggaggtgga tcggcttgcc cggtgccact tcgtaatcgt caacgggcag
     1321 accggccaga tcgaaaacgg tgactatgaa cgcaggatac ggaatggaat cgcagcagaa
     1381 gggctcgatc ccagggcata ctttccggaa gttcacgagc agtcactggt cctctcccgg
     1441 gcagtcccag gaacagaggt gaaattcaga tgagtaaatc cacaaaaggt ttcaagatcc
     1501 gactcgcaaa ggcttgcgaa cagaacaggc gggttccgca atgggtgatg ctccgcacca
     1561 agcgtgcagt ggtttcccat ccccggcgcc ggaactggag aaggagcgcc ctgaaggtgt
     1621 gaaaacatgg cagaaaagac gcaggaacag atctatatca tacctcttcg cgacgcacgt
     1681 cgtgcaccgc gatggaagag gagccatact gccatcaagg acatacggaa atatctcgag
     1741 aagcacttaa aaagtcacga tgtgaagctt gacaagagca tcaacgagag gatctgggac
     1801 cggggttcgg agaatccccc ctcacgtgtg cgtgtccgcg ccatgaaatt cgatgatggg
     1861 caggtccagg ctgagcttgc tgaggaatga gatggaaaaa acggtctcgt tcggcgggga
     1921 ttgcaacatc ggcgtgttca cgagagtttt tgatgacatc gccgtggtcc ctcccaatgc
     1981 acccggggac ttctgtgaag cattgaccgg attgctcggg gtggagattg tcgagaccac
     2041 cattcagggc agcgccatca tcgggtccct ccttgccgga aaccgccatg gatttgtggt
     2101 gagcggtttt gccgaagaga ccgagatcgc gcgcctgtca gaataccggg aactctgcct
     2161 gcttacgcac acgatgaacg ctgccggcaa tatcctgctt gccagtgact cccttaccct
     2221 ggcacatcct gacatgccgg aacagatggc agaggagatc ggtgacattc tcaaaacccc
     2281 ggtccacagg acaaccattg ccgggatcaa gacagtcggc atggcagcag tcgcctcggc
     2341 ccggggtatc atggttcacc cccgcaccac ccgccatgaa ctggccgggc ttgagagcct
     2401 gagtgacctt ccgatcggga ccggatcagt gaacatgggc agtggccttg tcgggaccgg
     2461 gctcgttgcc aattcacggg gatacgtggc agggcttgag accaccggat ttgaacttgg
     2521 acgaatcgag gatatatttg gattcctgga gagaacatga tgcagaacat gaaatttgaa
     2581 gtgacgggca ccttcatgat cggggatgaa tggcacccct accggaaagt gatcgaggcg
     2641 cccaacgagg tacaggcaaa agagcgaacc tttgcggtca tcgggagcaa gcatcgcctg
     2701 aaacgaaggt atatcaccgt caatgccgtt actcctgtga atggtgaata acgtggaccg
     2761 cgtggacccg cgggagcttc agtcgcttca gctgtacctg aacgactacc ggcagcaggc
     2821 ggagatattt acccggcagc ttgcgctcct tgaagagggg aggatggagg cactcgccgc
     2881 gatcgaggcg ctcgaagggg ctgcagcagc accggacagc attgtccttc tccagatcgg
     2941 tggaggggtg agcgttcgag cccgtatcct tgatcccgat cgtgtccttc tcaatatcgg
     3001 ggctgatgtg atcgttgagc ggccgaacgc tgcggcacag gactatctga aggaccgtat
     3061 cacggagatg gaagcatcag caaagaaggt tgccgagacc cttgaacggc tccgggtaca
     3121 gatgaacgag ctcgcccgca ggattgagct gggataccag caggcacaga tggcccagat
     3181 gcagcctcca cctgttccgg aagccgggga agagtgaacg gggagaacca tcggtatgtt
     3241 tgacgggctc cgtgctaagc tccagaagat ccagggcggg ctttcccaga gcatcgaatc
     3301 tgcctctgaa aaagaagcga tccctcccac cagttccgga gggatggaga agaatccgac
     3361 tatcctcgga aaggtaaaag tcctcctcac cgaccgcgag ttcctggtcg atgagaagga
     3421 tatcagagaa cccctctttg aacttgagat ggtcctcctc gagaatgatg tggcactccc
     3481 tgtcaccgat gagatcatca gccatatcag aaaggacctg acgggcagcc gcaggaagat
     3541 cggcgaatcc gttgattcca tcgttacctc cgcgttgcgg ggggcattgc ttgatgtcct
     3601 gggggagggt ttcgacctcg tctcctacct cagctcccat gaaaagccgg tcaagatcct
     3661 ttttaccggt gtgaacggca ccggcaagac caccactgtc gccaaggtcg gctctttcct
     3721 gaagaagaac ggattctctg ttgtcatcgg cgcaggagat accttccgtg caggagccct
     3781 tgaacagatc gatgtccatg cacagcgcct cgggatcaag atcatccagc accaggctgg
     3841 gggcgatccc tctgccgttc tctatgacac cgtacagtat gcggaggccc accatatcga
     3901 tgcggtgctt gccgatacgg caggcaggtt ccacaaccgg gtgaacctca tgaaccagct
     3961 cgacaagatc cggcgggtca tgaaacccga tctgatcgta tatgtcgatg aagcagtcgc
     4021 cgggaacgat gcagtggtcc gggctcacga gttcaagaag gcggtggggg cagacgccgt
     4081 ggtcctcaca aaagcagata tggatcccaa ggggggtgcc gcgatatcga ttgcacatac
     4141 tatcggaaaa cccctgatgt tcctcgggat tgggcagggc tatgatgacc tggtcccctt
     4201 ctcacctg
//
LOCUS       DAOOCO010000160         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_19991_length_4196_cov_5.043468, whole genome shotgun sequence.
ACCESSION   DAOOCO010000160 DAOOCO010000000
VERSION     DAOOCO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19991_length_4196_cov_5.043468"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1968
                     /gene="rqcH"
                     /locus_tag="PK154_08595"
     CDS             76..1968
                     /gene="rqcH"
                     /locus_tag="PK154_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HPW11154.1"
                     /translation="MATKEGMSGVDVHAVVAEWQKFVPLWVSKIYQFSSSHIVIRLNG
                     QEHARHLLLVEIGKRAHLVSMIPAPPKLPPPFAMLLRKHLEGGRVLGVSQPGLQRIIC
                     FDIGKQNTTFHLVIELFDQGNLVLCNDAWTIIQPLRPHRFRERDIIAGEVYRLPPADP
                     ATFSREAFGSFLKNDDRDVVRALAVGALLGGRYAEYLCRKAGVEKTIPAGEADADQLY
                     DALQALMHRAEEGIEPVLTGRECLPFPEDAGGGTAGTGSFNQALDRFFPGIPSSPAEA
                     APAAKGLSKEERIRKRQSEILAGYEQKAARTEKIAAMMYESYQDLGEIIRVLDEASRR
                     MSWQEIEQVLKKGRDGPAGRIRSVNPAEASVEVDIGERVTLYVHESLEANIGRYYDTL
                     KKLRKKIAGAKAAMDRPVAPPRQHRAGSPVMKKRWYHRFRWFSTSDGVLVIGGKDASQ
                     NEELVKRYMEGGDRFVHADVHGASVVIVKGRTGRMDEVAQFAASYSGAWRSGHATADV
                     YAADPGQVSKTPESGEYVSRGSFIVRGEREYFRDVPLAAAIGLQREPELAVIGGPPSA
                     VAHRTSLFVVLKPGTFEPNDTAKKVLRALRARIGEEEARTQKAVLNTDRIAAFVPPGG
                     SDIVEP"
     gene            1965..2990
                     /locus_tag="PK154_08600"
     CDS             1965..2990
                     /locus_tag="PK154_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HPW11155.1"
                     /translation="MKAEEGELQRGFGEIRLFPESLDDLWHLEHLIGPGDLVFATTLR
                     SVESASDKLRPDKPEKRPVRLGIRVERTEFHRFANRLRITGIIEHGDEAGSYHTLNIE
                     PGYEISVIKRWRPVDRERIDRAVKASVYEAVHIMTIEEGEAELFRMRQFGPEAVLTIT
                     SGSGKESGTGLRPSFFERVVSLLEGITGPLVIAGPGFVKDDLVRYLRSANPELTARSL
                     VVETRRIGIGAVQEVIGLGILERIHEDIQLGNEVRLFAELLSRIAKGLPAAYGRAEVK
                     RAIDFGACERLLIVDTLLRDEETIHLMDRAEQMNAGIVVFSSAFEPGRQLEGLGGIAA
                     LLRYHIG"
     gene            complement(3113..3379)
                     /locus_tag="PK154_08605"
     CDS             complement(3113..3379)
                     /locus_tag="PK154_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HPW11156.1"
                     /translation="MYLIIRCPGCHTFSYVDRYQQWKLCPRCGEAISVRQGPAYLEVE
                     DYSVAEQIIRQLERYLDSTQKKDLSSDEIAALRQQYADWIRSRI"
     gene            3490..>4196
                     /gene="cofE"
                     /locus_tag="PK154_08610"
     CDS             3490..>4196
                     /gene="cofE"
                     /locus_tag="PK154_08610"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPW11157.1"
                     /translation="MRDESIQVMGVRGLPLIQEGDDLCTLISERVDLQEGDIICIASS
                     VYSKAMGYTRSLGEIIPGERACRIAGRTGEDPRFIQGVLDASKEVLLEYPFVLSELPC
                     GHVGVRAGVDQSNIEKEHIILLPPDPMGAASDLKEGFRAVAGVETGVIVTDTCGRSFR
                     RGQTGHAIGWSGMTAIRDFRGDTDLFGHVLKITEEAVVDEIAGFANFIMGESNNAVPA
                     VVFRNCPTWNGHDNLYFS"
BASE COUNT          891 a         1147 c         1342 g          816 t
ORIGIN      
        1 ttacctcatc tgcactcatc gtgataccag tagtcctatt ttttagtctt taagcaatat
       61 gtaatttggt atcggatggc cacaaaggaa gggatgagcg gggtagatgt tcacgcggtc
      121 gtggccgaat ggcagaaatt cgtccccctc tgggtcagca agatttacca gttctcctcc
      181 tcccatatcg tgatccggct caacgggcag gagcatgccc ggcacctgct cctggtcgag
      241 atcgggaagc gtgctcacct tgtgagcatg atccccgcac ccccaaaatt accgccaccc
      301 tttgcgatgc tcctccgaaa acaccttgag ggtggcaggg tgctcggggt atcgcagcca
      361 ggtctccagc ggatcatctg ttttgatatc ggaaaacaga ataccacgtt ccacctggtg
      421 atcgaactgt tcgatcaggg aaatctggtg ctctgcaacg atgcctggac gatcatccag
      481 ccgctcaggc ctcaccggtt ccgggagaga gatatcattg cgggggaggt ataccgcctc
      541 ccgcccgccg atccggcaac cttttccagg gaggcattcg gcagcttcct gaagaatgac
      601 gaccgggatg tcgtgcgggc acttgctgtc ggggcattgc tcggagggag gtatgccgaa
      661 tatctctgcc ggaaggcagg cgtggagaag accattcctg caggcgaggc agatgcggac
      721 cagctgtacg acgccctcca ggcactgatg caccgtgcag aagaagggat cgaaccggtg
      781 ctcaccggga gggagtgcct gcccttcccc gaggatgcag ggggcggaac ggccggtacc
      841 ggcagtttca accaggccct cgaccggttc tttcccggga ttccgtcctc ccctgctgaa
      901 gcggcaccag ccgcgaaggg tctctccaaa gaggagcgga tacggaagcg gcagtctgaa
      961 atccttgccg gatatgagca gaaagctgcc cggactgaga agatcgcggc catgatgtac
     1021 gagtcgtacc aggatctcgg ggagatcata cgggtgctcg atgaggccag caggagaatg
     1081 tcatggcagg agatcgagca ggtactcaaa aagggcagag acggtcctgc aggaaggatc
     1141 aggtccgtga atcccgctga ggcctcggta gaggtggata tcggggagcg ggtgaccctg
     1201 tacgtccatg agagcctcga ggcaaatatc gggaggtact acgataccct gaaaaaactg
     1261 cggaagaaga tagccggtgc aaaagccgcg atggatcgcc cggtcgctcc ccccaggcag
     1321 caccgtgcag gatccccggt catgaagaag cgttggtatc accggttccg gtggttttcc
     1381 accagtgacg gcgtcctggt gatcggcggg aaagatgctt cgcagaacga agagcttgtc
     1441 aagcggtaca tggaaggggg cgaccgcttt gtccatgccg atgtgcacgg tgcaagtgtc
     1501 gtgatcgtga aagggaggac tggcaggatg gatgaggtgg cacagtttgc cgcatcatac
     1561 tcgggggcat ggaggagcgg ccatgccacc gctgatgtct acgcggcaga tcccggccag
     1621 gtgagcaaga cacccgaatc cggagaatac gtgagcaggg gttcttttat cgtcaggggt
     1681 gaacgggaat atttcaggga tgtgccgctt gcagccgcca tcggacttca gcgtgagcct
     1741 gaactggcgg tgatcggagg ccccccctcc gcggtggccc accggacctc tctcttcgtg
     1801 gtactgaaac ccggcacgtt cgaaccgaac gatacggcca agaaagtgct ccgggcattg
     1861 cgtgcgagga ttggcgagga agaggcaagg acacagaagg cagtcctgaa cactgaccgg
     1921 atcgcggcat ttgtcccgcc aggcgggtct gacatcgtgg aaccatgaaa gctgaagagg
     1981 gagaactgca gcgcggattc ggcgaaatac gcctgttccc ggaaagcctg gacgatctct
     2041 ggcacctgga acacctgatc ggtccgggcg atctcgtctt tgccacgacg ctccgttcgg
     2101 tggagtcggc ttcggacaag ctgcgcccgg acaagccgga gaagcggccg gtccggctcg
     2161 gaatacgggt cgaacgcacc gaattccacc ggtttgccaa ccggctccgg atcaccggga
     2221 tcatcgaaca cggggacgag gccggttcct accataccct gaatattgaa ccgggatacg
     2281 agatctcggt catcaaaagg tggcgtcctg tcgatcggga acggatcgac cgggctgtga
     2341 aggcatcggt ctatgaagcg gtccacatca tgacaatcga ggagggcgag gccgagctct
     2401 tccgcatgcg gcagttcggc ccggaggcgg tccttaccat cacctcggga agcggaaagg
     2461 aatcgggaac cgggctccgg ccgtcttttt ttgagcgggt ggtctccctt ctggaaggga
     2521 tcacggggcc gcttgtcatt gcggggcccg gatttgtgaa ggacgatctg gttcggtatc
     2581 tccgttccgc taatccggaa ctcaccgcac gatccctcgt ggtcgagacc cggaggatcg
     2641 ggatcggggc ggtccaggag gtgatcgggc ttggcatcct tgagcggata cacgaggaca
     2701 tccagctcgg aaacgaggtc aggctgtttg ccgagctcct ctcccggata gcgaaaggcc
     2761 tcccggccgc ctatgggagg gcagaggtga agagagcgat cgattttggt gcctgtgaac
     2821 gcctgctcat cgtggacacc ctcctgcgtg atgaggagac catccacctg atggacaggg
     2881 cggagcagat gaacgccggc atcgttgtct tttccagcgc ttttgagccg ggcagacagc
     2941 tggaggggct cgggggtatc gctgccctgc tgcgatatca tatcggataa gtcccaggaa
     3001 cagagcaaag aacaagagcg aagagccctt tttccatccc agggagaaca gcacgatacc
     3061 cttctcacca gggggaggta caggacagtg gagggaagca ggacagccgg ggtcagatcc
     3121 tgctccggat ccagtccgcg tactgctggc ggagtgcggc aatctcgtcg gatgacaggt
     3181 cctttttctg ggtgctgtcg aggtagcgtt cgagttgcct gatgatctgc tctgccaccg
     3241 aataatcctc tacttcaagg taagccggcc cctgcctgac actgatcgcc tccccgcacc
     3301 gggggcagag tttccactgc tggtaccggt ctacgtaaga gaacgtatgg cagccgggac
     3361 accggatgat gagatacatc agcaatggct gattgcatcc tttctggtat ataaatatcg
     3421 tgctcccggg atccccctgg ctacggcagc tgtattttat catcctgttt tacctacgct
     3481 agaaaagata tgagagacga gtcgatccag gtcatgggag tccgcgggct cccgctgata
     3541 caggaagggg atgatctctg caccctgatc agcgagcggg tagatctgca ggagggcgat
     3601 atcatctgca tcgcttcatc ggtctattcc aaggcaatgg gatacacgag gtcactcggg
     3661 gagatcattc ccggcgagcg ggcgtgccgg atagccggaa gaaccgggga agacccccgc
     3721 ttcatccagg gggtgcttga cgcctcaaag gaggtcctcc tggagtatcc ctttgtcctc
     3781 tctgagctgc cctgcggcca tgtaggggtg agggcggggg tcgatcagag caacatcgag
     3841 aaggagcaca tcatcctgct cccccctgat cccatgggtg ccgcatccga tctgaaggaa
     3901 gggttccgtg ctgttgcagg agtcgagaca ggggtgatcg tgaccgatac ctgcggccga
     3961 tcgtttcgga ggggacagac cggccatgcc atcgggtgga gcggcatgac agcgatccgc
     4021 gatttccggg gggatactga tctcttcggg catgtcctga agatcactga agaggcggtg
     4081 gttgacgaga tcgccgggtt tgccaacttc atcatgggag agagcaataa tgcggtcccg
     4141 gcagtggtct tccggaactg tcccacctgg aacggccatg acaacctgta cttctc
//
LOCUS       DAOOCO010000161         4185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_20059_length_4185_cov_4.788620, whole genome shotgun sequence.
ACCESSION   DAOOCO010000161 DAOOCO010000000
VERSION     DAOOCO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20059_length_4185_cov_4.788620"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK154_08615"
     CDS             <1..397
                     /locus_tag="PK154_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11158.1"
                     /translation="TEMISPLPVYRYDYGEASFALFGDELSQISSALEGMKQGGSEKI
                     VFENSDQLQRDMTPEQFAQMGGNSTTAVPGDQLLLAFTTNPMLNMDDNSTPQYALRTS
                     VITGKTSENVTVNYGYPSADISIVRLNRA"
     gene            444..701
                     /locus_tag="PK154_08620"
     CDS             444..701
                     /locus_tag="PK154_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW11159.1"
                     /translation="MLRVISFFQEGCMACDEQDPINREVEEALGIRFEVINPLQERSY
                     IDTYHLQVTPTILIVKDDMVVERFEGVVHREQLEEAIRHHL"
     gene            complement(696..1307)
                     /locus_tag="PK154_08625"
     CDS             complement(696..1307)
                     /locus_tag="PK154_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HPW11160.1"
                     /translation="MEKGRQVHRLLKMQMRIFCPECKDEQDHIVLSQSRQTRVRCTTC
                     GTVHQLAREKEQPPLHVKTIVSEEGTSRICAIELLPEEPCQIGDHHVAVCGEEYIGVE
                     VTAIERGQHRVRKARAKDITALWSRKIEEVLVRVSVHDGRSTVPLAIRVSGEEPFVVG
                     EVYRVGNRRFRIAHMKMRDGAALRHEGQKGIARKIKRVYGYPL"
     gene            1344..1898
                     /locus_tag="PK154_08630"
     CDS             1344..1898
                     /locus_tag="PK154_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HPW11161.1"
                     /translation="MKGVPLGSGQLRSGMTLTECTTLTETIRCRGHRNVLSLHAGTFM
                     ITKDLHLTCKGDCIIAIGADKGAADLSPAFRKALSIDDAMLFTKLTCRGISITAFSWG
                     SAGMTLLHPTDMVWRKSTYVCGRTVGIGSDLAAKDLPRELVDCLRNGEELLVEMTVAT
                     GGHEGYDQFGSMLVPSQHRDLQFR"
     gene            complement(1860..2042)
                     /gene="xseB"
                     /locus_tag="PK154_08635"
     CDS             complement(1860..2042)
                     /gene="xseB"
                     /locus_tag="PK154_08635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPW11162.1"
                     /translation="MTKTYEELVNELKEIIKTIEDNETGLDESIALYERGAAIIRQCE
                     DLLASAELKITMLGRD"
     gene            complement(2026..3279)
                     /gene="xseA"
                     /locus_tag="PK154_08640"
     CDS             complement(2026..3279)
                     /gene="xseA"
                     /locus_tag="PK154_08640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPW11163.1"
                     /translation="MVYAQKGLPGAPEEHGIAVFSISEISSVISSILDDTRIQDIWIR
                     GEVTNFRPHVSGHRYFSLSERGQNCGAMINCVMWRSDAKRIGGEFRDGMDVIAFGSIT
                     HYPPQGKYQFIARELRHAGVGEKHLLVERWKAELAAEGLFSEERKRPLPRFPMRVGVV
                     TSETGAVLQDIRNIIARRFPLEIIVSPTAVQGESAHLEIAAALRRIDGRADVIIVGRG
                     GGSFEDLFPFNHPDVVRALSRCSVPVVSAIGHEVDITLADFAADVRAPTPSAAAELVV
                     QDREVLREGLTLYSRKLGADLIGKLDRASRELSDLRTRIAPGRMQRRIADRQQDAAVM
                     AERLSRALSGKIAIQRLVISGFLEKIENRNPLRMLERGYAIIEKDGRAVKSVQAIEPE
                     TEIQARMKGGKLDLLVRSVTHDKDL"
     gene            3320..4141
                     /locus_tag="PK154_08645"
     CDS             3320..4141
                     /locus_tag="PK154_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPW11164.1"
                     /translation="MVRFAVSSMFFHEYTLDEIFDFVAEAGLDTLEFWVETPDFWLRG
                     QPLPELISCLSSRPALHPLTMHAPILDLNPCSINPRVAEASVEYCLASLMIAERIGAE
                     VLTVHPGRRTAKRPPGAADFRRFDHYLSLLESAARRTSVRVSIENMEKKVNSLLCTPE
                     SVREILDREHWLSFTLDVSHALGVSLEEVATYIDLCGERLGNVHLSGANGGTMHLPVE
                     DNPDITTILHLLSDAGYQGVITFEFEDRNFNHDLSSEEKILFLARQAAVVRESLG"
BASE COUNT          870 a         1249 c         1105 g          961 t
ORIGIN      
        1 cactgagatg atctcccccc tcccggtcta tcgctatgat tatggggagg cgagttttgc
       61 tctctttggc gatgaattaa gccagatatc ttcagccctc gaagggatga agcagggagg
      121 atccgaaaag atcgtctttg aaaattccga ccagctccag cgggatatga ctcctgagca
      181 attcgcccag atgggaggaa acagcaccac ggcggtgccg ggagaccagc tactcctcgc
      241 ctttacaacc aaccccatgc tcaatatgga tgacaactca accccgcagt atgccctccg
      301 cacctctgtc attactggaa agaccagtga gaatgttacc gtgaattatg ggtacccgag
      361 cgccgatata tcgatcgtcc gtctgaaccg cgcctgatgt ccgggcacgc ctatttatag
      421 cccggctccc ttttttacag tgtatgctca gggtgatatc gttctttcag gaaggatgca
      481 tggcctgcga tgagcaggat ccaataaata gggaagttga ggaagcactc ggaatacgat
      541 tcgaggtgat caatcctttg caggaaaggt cctatatcga tacctaccat ctccaggtga
      601 ctccgactat tctcatcgtc aaggatgaca tggttgttga acggtttgaa ggagtggtgc
      661 accgggaaca gctcgaggaa gcgatccggc atcacttata gtggatatcc atagacccgt
      721 tttatcttcc gtgcaatccc cttctggccc tcgtgtcgga gggcggcgcc atcgcgcatc
      781 ttcatgtggg cgatccggaa acgtcggttc ccgacccggt acacctctcc gaccacaaac
      841 ggctcctctc cgctgacacg gatggcgagc gggacggtcg atcgcccgtc atggactgat
      901 accctgacca ggacctcttc gatcttccgg ctccataagg cagtgatatc ctttgcccgt
      961 gctttcctga cccggtgctg cccccgttcg atagccgtga cctcgacacc gatatactcc
     1021 tcgccgcaga ctgctacgtg atggtcgccg atctggcatg gttcttcggg caggagctcg
     1081 atggcacaga tgcgggaggt gccctcttcc gagactatcg tctttacgtg aagcggaggc
     1141 tgctcttttt cccgtgccag ctggtgtacg gtaccgcagg tcgtgcaccg aacccgtgtc
     1201 tgcctggact gcgacagaac aatatgatcc tgctcgtcct tacattcagg acaaaatatt
     1261 ctcatctgca tcttcaagag ccggtgtacc tgtcttccct tctccattat taggattggc
     1321 ctggaaggag catgggcatt atcatgaaag gcgttccact gggatcagga caactccgat
     1381 ctggtatgac cctgacagaa tgcacgaccc tcacagagac gatacggtgc cggggccacc
     1441 ggaatgtcct ctcgctgcat gccggcacct tcatgatcac caaagacctg cacctcacct
     1501 gcaaggggga ctgcattatc gcgatcggtg ctgacaaggg tgccgcggat ctctcccccg
     1561 cattcaggaa ggctctctca atcgacgatg ccatgctctt tacaaaactt acctgccggg
     1621 gcatctcgat caccgccttc tcatggggat cggccgggat gaccctgctc cacccgacgg
     1681 acatggtctg gcggaagagc acctacgtct gcgggcggac ggtcgggatt ggatcggacc
     1741 tggctgcaaa agacctgcct cgcgagctgg tcgactgcct tcggaacgga gaagaactcc
     1801 tcgttgaaat gacggtggcc actgggggcc atgaaggata cgatcagttc ggcagcatgt
     1861 tagtcccttc ccagcatcgt gatcttcagt tccgctgaag cgagcaggtc ttcgcactgc
     1921 ctgatgatgg cagcgccgcg ttcatacaac gcgatactct catcaagacc ggtctcgtta
     1981 tcctcaatcg tttttatgat ctcttttaac tcgttcacca gttcttcata ggtctttgtc
     2041 atgtgtcacg ctcctgacga gcaggtcgag cttccccccc ttcatccgcg cctgtatctc
     2101 agtttccggt tcgatcgcct gcactgattt tactgccctc ccgtcctttt cgataattgc
     2161 gtatccccgc tcaagcatcc gcagcggatt cctgttctcg atcttctcca gaaacccgct
     2221 tatcaccagc cgctgaatag caatcttccc ggagagcgcc cttgaaagcc tctcggccat
     2281 gaccgccgca tcctgctgcc ggtctgctat ccgccgttgc atccttcccg gggcaatccg
     2341 ggttcggagg tcggacagct cccgtgatgc ccggtccagt ttcccgatga gatcggcccc
     2401 cagctttctc gagtaaagag tgaggccttc acggagcacc tcccggtcct gcacgacgag
     2461 ttcggctgct gcggacgggg ttggtgcccg gacatcggca gcaaaatccg caagagtgat
     2521 atcgacctca tggccgattg cgctcacgac cgggacgctg caccgtgaca gtgcccggac
     2581 tacgtcagga tggttgaatg gaaagaggtc ctcgaaactc ccccctcccc tcccgacaat
     2641 gatgacatca gcccggccat ctatcctccg cagggcagca gcaatctcca ggtgggctga
     2701 ttctccctgg acagcggtcg gtgagacgat gatctcaagg gggaaccgcc gggcaatgat
     2761 attcctgata tcctggagca ccgcccccgt ctctgatgtt accaccccca ctctcatggg
     2821 aaaccggggg agggggcgct tcctctcttc agaaaagagc ccctctgccg caagttcggc
     2881 tttccatcgc tcaacaagga gatgcttctc ccccaccccc gcatgcctca gttccctggc
     2941 gatgaactgg tatttcccct gcggggggta atgggtgatg gatccgaaag cgatcacgtc
     3001 catgccatcc ctgaactccc ccccgatgcg ttttgcatca ctccgccaca tgacgcagtt
     3061 gatcattgcg ccacaattct gtccccgttc gctcagggag aaataccggt ggccggagac
     3121 atggggcctg aaattggtca cttccccccg tatccagatg tcctggatac gggtgtcatc
     3181 gaggatggag gatatgaccg atgagatctc ggagatcgag aagacagcga ttccatgctc
     3241 ctcaggcgct ccggggagtc ccttctgggc gtataccatc aacaaatatt atggcaacgg
     3301 cggaatatga aataggatca tggtacgctt tgccgtgtca agcatgttct tccatgagta
     3361 taccctagac gagatctttg atttcgtcgc tgaggcaggc cttgacacgc tggagttctg
     3421 ggtcgagact cccgatttct ggctccgggg acaacctctt cccgagctca tcagctgcct
     3481 ctccagccgg ccggcactac accccctgac tatgcacgca cccatccttg acctgaaccc
     3541 ctgttcgatc aatccccggg tggcagaagc ttccgtggaa tactgtcttg cctccctgat
     3601 gattgcagaa cggatcggtg ccgaagtgct gaccgtgcat ccagggagga ggacggcaaa
     3661 gcgacctcca ggtgcggctg atttcaggag gtttgaccac tacctgtccc tgctcgagtc
     3721 tgctgcccgg agaaccagtg tgcgggtgtc gatcgagaac atggagaaaa aggtcaactc
     3781 cctcctctgc acccctgaat cagtaaggga gattcttgac agggagcact ggctctcgtt
     3841 cacccttgat gtctcccatg cccttggcgt gtcactggag gaggtagcaa cctacatcga
     3901 tctttgtggg gagcgactgg gcaatgtcca cctgtcgggc gcaaacggtg gaaccatgca
     3961 tctgccggtc gaggataacc ccgatatcac caccatcctt catctgcttt ccgatgccgg
     4021 gtatcagggg gtcatcacgt tcgagttcga ggaccgaaat tttaaccatg atctctccag
     4081 cgaggagaag atcctcttcc ttgcacggca ggctgccgtt gtgagggaaa gtctcggata
     4141 aacgctggtt ttataaaaag ggaatactat tattgtaata gtttt
//
LOCUS       DAOOCO010000162         4168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_20188_length_4168_cov_4.136640, whole genome shotgun sequence.
ACCESSION   DAOOCO010000162 DAOOCO010000000
VERSION     DAOOCO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20188_length_4168_cov_4.136640"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK154_08650"
     CDS             complement(<1..536)
                     /locus_tag="PK154_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11165.1"
                     /translation="MMKCPLPALLLALIAAAMVILPVSAFDVSGNSDDLVFFSVSDAG
                     LHTITMHYTGSSPFVVTLEDDQGIPLEYLALEAGSFQGQVKVDLDPGDYSIVVTASGP
                     WSIDIEAPVPVTAITTPPTPYVTTAIPTITPTPTPTPCPDGVCPGSLPTTEPTGIPTG
                     SPTTLPNCWPNCEPTTMPT"
     gene            794..1375
                     /locus_tag="PK154_08655"
     CDS             794..1375
                     /locus_tag="PK154_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11166.1"
                     /translation="MEPNDPVYWKQRAMDWYNDDKIEEARECWINALDLDPDDIVVWK
                     LLRNASQDAGDMNGAEFCSEKIRELEKKNAAEPDSIPIPPVPKDDDRILQRVSESLGP
                     LPVERKSRRHGLEWIAMFITGVTQIENHQKYKGIFLIAGVIGFVALSLLTQDISASIM
                     TGFLYAFGVIDSYTILAGKNGTRELGSVQSADW"
     gene            complement(1495..2061)
                     /locus_tag="PK154_08660"
     CDS             complement(1495..2061)
                     /locus_tag="PK154_08660"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW11167.1"
                     /translation="MEKVALGPMPYMSVMPTLIVGATVKGKPNYMTAAWATVACMAPP
                     MVCVAVNKGRFTARGIEENQTFSLNVPSVHQVVETDHCGLVSGNNEDKSTVFHSFYGE
                     LKTAPLAEECPVNIECRVFRSVDCGSHALYIGEVVEIHADKSCVTEGKLDIDKIRPVI
                     YAQYAYYGIGNQIDKAFFAGKKYKKKNY"
     gene            complement(2111..2368)
                     /locus_tag="PK154_08665"
     CDS             complement(2111..2368)
                     /locus_tag="PK154_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11168.1"
                     /translation="MIISVFKVFYPVPVMSDAGYGIIDIDGESLFHHHYTEIWSPVTG
                     LPSVAPTSLCRNPLIPALFIGDESDQETAGLMKREKTNHRF"
     gene            3324..3539
                     /locus_tag="PK154_08670"
     CDS             3324..3539
                     /locus_tag="PK154_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11169.1"
                     /translation="MITAADIKTMADRWKRASRPLKIQDQRYGHRLVQMLAEYNGEEI
                     KRFDDPLEAAAFIVLVRMVKELDLVDY"
     gene            3841..>4168
                     /locus_tag="PK154_08675"
     CDS             3841..>4168
                     /locus_tag="PK154_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPW11170.1"
                     /translation="MKRLGHLAIVAHDMGLIFEFLALVTLVPFIVLIIYREWEMILPM
                     GTVPFLFLASGFLISKVPRKPYIPSLSVALVAVALTWLAAAVIGAVPFVLALEVPWTD
                     GIFESMS"
BASE COUNT         1014 a         1042 c         1084 g         1028 t
ORIGIN      
        1 gtcggcatgg tagtcggctc gcagtttggc cagcagttcg gtaacgtggt gggtgaacca
       61 gtggggatgc cggtcggttc agtggtagga agtgagccgg gacacacccc atccggacag
      121 ggggttggcg tgggggtagg ggttatagtg ggtatcgcag tcgtcacata cggagtagga
      181 ggcgtggtga ttgccgtcac agggacggga gcttcgatat caatggacca tggacccgaa
      241 gcggtaacca cgattgaata atccccggga tcaaggtcta ccttcacctg gccctggaac
      301 gaaccggctt cgagagcgag gtattcgagg ggaattccct ggtcatcttc aagggtcacc
      361 acgaaggggg aagagccggt atagtgcatg gtgatagtat ggagacctgc atcagagaca
      421 gagaaaaata ccaggtcatc ggaattcccg ctcacatcga aagccgatac cggcaggatc
      481 accattgctg ccgctatcag ggccaggagg agtgcaggga gaggacactt catcatattc
      541 atccctgaag atgtaatcat tcagatgttg aaatatattt tggatggagt tgtttcaata
      601 caaacctcat gcgatagcct tcttgcgcat tgggagtgaa aaacaaaggc tggttgtggc
      661 ttgttcacgc catcatttca caggaggata gtaatgcctg atgaagtctt cacttgatac
      721 gataacatgc atactcgcga ttaatatata gaaaaattac gatatctttg attattcgtg
      781 gattttgtga attatggagc ctaatgaccc ggtatactgg aaacagaggg caatggactg
      841 gtataacgat gacaagatcg aagaggcacg agagtgctgg attaatgctc tggatcttga
      901 tcctgatgac atcgtggtat ggaaattgct caggaatgca tcacaggatg caggggatat
      961 gaatggcgct gagttctgca gtgaaaaaat tagggaactg gagaagaaaa acgctgcaga
     1021 accggattcg atacccatac cccctgtccc aaaggatgat gaccgcatcc ttcaaagggt
     1081 atccgaatcg ctcggtccgt tacccgttga gcggaaatcc cgcagacacg gcctggaatg
     1141 gatcgctatg ttcatcaccg gggtgaccca gatcgagaac caccagaaat acaaagggat
     1201 attcctcata gccggggtta tcggctttgt tgcactcagt ctcctcaccc aggatatctc
     1261 agcatcgatc atgacggggt ttctctatgc ctttggggtc attgacagct ataccatact
     1321 tgccggcaag aatggaacaa gagagttggg atcggtgcaa tctgcggact ggtagagcat
     1381 cggctgatcc ctctgctctg ctcccctgac aggtggatca atgcagttca gaacggcctt
     1441 atatcagaag tgcttttgag ggaacgatta tccctgacag taaaaaatag ggaatcagta
     1501 gttcttcttt ttgtacttct tcccggcaaa gaacgcctta tcaatctgat tgccaatacc
     1561 atagtacgca tactgggcat agatgacagg acggatcttg tcgatgtcca gcttcccttc
     1621 agtcacgcag gacttgtcag catggatctc cacgacttcg ccgatataca gcgcatggct
     1681 tccgcaatcg accgatcgaa acaccctgca ctcgatattg accgggcact cctctgcaag
     1741 gggtgcggtc ttcagttccc cgtaaaatga gtggaacacg gtggacttgt cctcgttatt
     1801 tcctgaaaca agcccgcagt ggtcggtctc caccacctgg tgcacggaag gcacattcag
     1861 actgaatgtc tggttctctt caatccctcg tgcagtgaag cgacccttgt tgaccgcgac
     1921 gcagaccatc gggggcgcca tgcaggcaac tgttgcccat gcagcggtca tgtagtttgg
     1981 tttccctttc accgttgcac cgacaatcag ggtgggcatc acactcatgt atggcatcgg
     2041 gccgagcgct actttctcca tgattcctct tcgcatacct gttatggaaa gaacggctta
     2101 aaagtatttt tcagaaacgg tgattggttt tctctctctt catcaggccg gcagtttcct
     2161 gatcactctc atcaccaatg aataaagcgg gaatcagggg attccggcaa agactggtcg
     2221 gagcaactga tggaagaccc gtgactggag accatatctc ggtatagtgg tgatgaaaga
     2281 ggctttcacc gtcaatatcg attatgccat accctgcatc tgacatgaca gggacaggat
     2341 agaatacctt gaaaacagaa atgatcattg cataccttat tctggtgaaa accaatgagc
     2401 gaggaactcg agaaagtgat catcattctc ctgctattca gttttatcgt tctcttcgcc
     2461 atgtttgcca cattggtgta tggattattc aacaccactt agtccgggca gagcgccggc
     2521 cggcgatacg gattctttct cccgaaaaag aacatgatgc acctgcagtc gtcggttccc
     2581 cccttagctc tggtagaaag ggtataaagg atatagaaac ccactcgtgc atcatggggg
     2641 tgacactccc gcagagcctg attcttcccg acgtaggagc gcgcgttaga gtatacgttt
     2701 ttttcaagga actgcttgcc aggtatccga ttgtcacgag agtaaagcta aaatatttga
     2761 agaattcaat ctcaatcgtc cacctgtttc gccaccttaa caaaacagtg ctggatagga
     2821 gcagtgggat ccagtaatgg aatcccatgc tgctcctttt ccttcgatcc ctcttttgcg
     2881 tcgcgggccg aaatccgaca gagaagatta tgccgggctt tgtatggtgc tcaccctact
     2941 aatctgcccg ctctcccaga cagggtacct gtcgatctcg aacaatttcc cacagagcca
     3001 ctgcatggac ttttcctgct atagaagacg agtaaatatg gagccgttct gattcgggaa
     3061 caaccaggtc gagagtacca tcactaccgg ggatggttac acaagagggc tacagtacca
     3121 tcgccaccgt actcatgctg catacctgtt cctccaggga gagtaccttg accacaccca
     3181 ctccggacct gatggatcag acctgcctcg cccggaagcg ctctggggga tgacagcaga
     3241 tggatcctct ttagcaatgc atgagaagac tttcaccctg cacatggtga tccctgatac
     3301 cccctggcat ctgctctgat catatgatca ctgcagccga tatcaaaacc atggcagatc
     3361 ggtggaagag agccagccgg ccattgaaga ttcaggacca gagatacggc catcggcttg
     3421 tacagatgct cgcggaatac aatggtgagg agatcaagcg gtttgatgac ccgcttgagg
     3481 cagcagcatt tatcgtgctt gtcaggatgg tgaaagaact tgatctcgtg gactattgat
     3541 acggggggat cgggtttctg gtgaactgcc gcttttgatc tctgccggga aggcttatgc
     3601 agaagttgct gctgatacaa tgcaccattc cttccagtat gcgaggggat cagtgccttc
     3661 tctcatggtc tctgcatctc ttgtgtgaga aagagatatc ccggataatc ccccaaaggc
     3721 ccgctctttc gtttgttacc atgaacagac catatcttgt cccctgaagc gcatcatggg
     3781 gtgcctgcac gctcccgata gagaacacta ttgcggatgg ggacataacg ttcatagtac
     3841 atgaaacgac ttgggcacct ggcgatcgtt gctcatgaca tggggctcat cttcgagttc
     3901 ctcgccctgg tcaccctggt cccgttcatc gtcctcatca tctaccgcga gtgggagatg
     3961 atcctcccga tgggcacggt cccgtttctc tttctcgcat ccgggtttct catctccaag
     4021 gtgccgagaa agccttatat tccctccctc tctgttgcac ttgtcgcggt tgccctgacc
     4081 tggcttgccg cggctgttat cggtgctgtt ccctttgtgc tcgccctgga ggtaccatgg
     4141 acagacggta tcttcgaatc catgtcag
//
LOCUS       DAOOCO010000163         4133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_20406_length_4133_cov_4.836930, whole genome shotgun sequence.
ACCESSION   DAOOCO010000163 DAOOCO010000000
VERSION     DAOOCO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20406_length_4133_cov_4.836930"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1070)
                     /gene="budA"
                     /locus_tag="PK154_08680"
     CDS             complement(258..1070)
                     /gene="budA"
                     /locus_tag="PK154_08680"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HPW11171.1"
                     /translation="MNAPKILTLGGLVVILVLLALLFSSWLSGGISRTENRDTLYQLS
                     TIDALQQGVFDGIQPVGEIKKHGDFGIGTFNALDGEMIVLEGIVYQAASDGSVVIVPD
                     TITTPYTTVTFYREDFSLTTEEAMNFSTFSNVTGTYLPIENMVYAVRIHGMFPFMKVR
                     SVPPQQKPYPTLSEAVENQSVFVYTDISGWVVGTFTPSFFEGINIDGYHLHFISDERQ
                     KGGHILDFTVPAGAVIGYDITPDFTLVLPTSGDFTTVDLSEDLSGQLIGVEH"
     gene            complement(1126..1839)
                     /locus_tag="PK154_08685"
     CDS             complement(1126..1839)
                     /locus_tag="PK154_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPW11172.1"
                     /translation="MCGRFTISFVIGLSERFQARNPSFELIPRYNIAPSQDVPVVVRA
                     HPDDAVNEIRMMRWGLVPSWVKDPSRSPKPINARADTLNEKPMFRNLLQTKRCLIPAT
                     GFYEWKKEVWGRTPYYIRMRDNSLFAFAGLFDTYRAPDSGKISTFSIITTEPNAVVSP
                     YHDRMPAILKGEDEERWLGSGVFCRDELSALLHPYPASGMEAYAVGKKVNNPDAEGQD
                     LILPVPSLTNQSWDRKERG"
     gene            2203..3087
                     /locus_tag="PK154_08690"
     CDS             2203..3087
                     /locus_tag="PK154_08690"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HPW11173.1"
                     /translation="MNEKILAVILALSLLANLGMGLYIANDHLYGPGMLRKEYETDKG
                     ADRTIGPSGESGRTIPVADILDTISGQEKRSASLQGLAVITTINYSRQGQFIYQEKNE
                     TGTTINVTVDIIPGEGRVLVQTKPLMGIVFQDAANTAVAVARNRTTADFSGSDVIFSI
                     TSEGVIPAVDGASAGALMTIITRAAATGEPLWENRTLTGTITPDGHIGAIGGVIEKAR
                     AAKVAGKDLLLLPRENAYIVVLNESSRSMGGLRMTRLVYETVPAKEYIESTMGIRVEF
                     VDTIDEVEAYLFRTGQSG"
     gene            complement(3098..>4133)
                     /locus_tag="PK154_08695"
     CDS             complement(3098..>4133)
                     /locus_tag="PK154_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW11174.1"
                     /translation="TISASFRVIEELHDRYYINASAGNGGVIAPSGQLEVVPKETITF
                     TITASPGYTIQTIVVDGENQEVSDSFTFTNISGNHTITASFIASGGDGGTVGKGTLYV
                     TSIPRGALITIDGVVKGQTNAIISNVAAGIHDVTLTRSGYHPETKQVDVKAGGMNSVA
                     FTLKESGEPGTGTGTLYISSIPSGAAITIDGVESGQTNMYVRNIASGIHTITLTKPGY
                     LPGTKQVSVNTGGVEWVSFTLKKGGDIEPGTGDGTTTLTGTGTLNVKSVPKGAVITID
                     GVESGQTNVLLSKIAAGSHNVTVSKDGYQPETKQVDVKLGGRNSVSFTLQPFDGSGIV
                     QVSDLFPFSL"
BASE COUNT          983 a         1132 c         1075 g          943 t
ORIGIN      
        1 agaacctctc cccggtacaa gatgatgatg ccaccgacag catcaatata ccggaccaaa
       61 acagctgagg accggggttt cccaaagaaa aaggttcctg ccggacggac catcaaaatc
      121 ccggaacgtc agcccgggac atctctcgta ttgggtattt taaaagcaca ccctcttcca
      181 gaccggcgcc cttgttccac aagaatgttt tcctgtttcc tgacgcagga cggttgttcc
      241 tgaaaaggtt gacagggtca gtgctcaacc ccgatcagct gcccgctcaa gtcctcagaa
      301 aggtctacgg tggtgaagtc cccgcttgtt ggcaggacca gagtaaagtc aggggtgatg
      361 tcatatccga tgacagctcc ggccgggacg gtgaagtcga gaatatgacc gcctttctgc
      421 cgctcatcgc tgatgaagtg cagatggtac ccatcgatat tgatcccttc gaaaaacgaa
      481 ggagtgaagg ttcccaccac ccacccggag atatcggtat acacgaatac cgactggttc
      541 tctactgctt ccgacagggt cgggtatggc ttctgctgag gcgggaccga ccgtaccttc
      601 atgaaaggaa acataccgtg gatcctgacc gcatacacca tattctcaat gggaagataa
      661 gtccccgtca cgttcgaaaa ggtcgaaaag ttcatcgcct cttcggtagt aagagagaaa
      721 tcctcgcgat aaaaggtaac ggtagtatat ggtgtggtga tggtatccgg aacgatgacg
      781 acacttccat cagatgctgc ctggtagaca attccttcca ggactatcat ctcaccatcg
      841 agggcattga aggtgccgat tccaaaatcc ccatgttttt ttatctcgcc caccggctga
      901 atcccgtcaa agacaccctg ctgcagcgca tcgatggtcg agagctggta cagcgtatcc
      961 ctgttttccg tgcgggagat tccccctgac aaccaggagg agaagagaag agccaggagg
     1021 acaaggatga caacaagtcc gccgagggtc aggatttttg gagcattcat acgaattcct
     1081 cttagcagaa caatacaaaa ggggagttat aaaggtgtgc cggaatcatc ccctctcctt
     1141 tctatcccag gactggttgg tcagggaagg gacaggcagt atcagatctt gtccttcagc
     1201 atcaggattg ttgacctttt ttccgacagc gtatgcctcc atcccgctgg caggataggg
     1261 gtggagcagg gctgacagtt catccctgca aaaaaccccg gaaccaagcc agcgttcctc
     1321 gtcctctcct ttgaggatgg caggcatcct gtcatgatag ggcgaaacaa ccgcatttgg
     1381 ctcggtggtg atgatcgaga aggtgctgat cttcccgcta tcgggagccc ggtacgtatc
     1441 aaaaagaccg gcaaaggcaa agagggaatt gtccctcatc ctgatgtagt atggtgtcct
     1501 cccccagacc tccttcttcc attcatagaa cccggttgcc gggatgaggc atcgtttcgt
     1561 ttgcaggaga ttacggaaca tcggtttttc gttgagggtg tcagccctcg cattaatcgg
     1621 ttttggagaa cgggagggat ccttcaccca cgatggcacg agcccccacc tcatcatcct
     1681 gatctcattt actgcatcat ccgggtgagc ccggactacc acagggacgt cctgtgaagg
     1741 agcaatatta taacgaggga tcagctcaaa ggatggattc cgtgcctgaa acctctctga
     1801 aagcccgatc acaaacgata tagtgaacct gccgcacatc actgaccacc tgattgtccg
     1861 attcgcagtc tcttattctg ttttcatcat gatcagtgat gaattctggt acgatccgca
     1921 gccgccagtc ctaaagatat ggccggctcc caaaccccct cctcctatcg ttcgtgccat
     1981 gctgctcact cccttacaag actccctgac acgagaggaa cctttggttg tattttgtct
     2041 ggatccctgc cgaagggatc acccctgtgc ctggagagcg atcagcatcc cactattcca
     2101 acgtgaaaat tcccagggtt ccgtaccgtc atcgtacctt ccggaaggtg ggttccgctc
     2161 ggaaacctat tatacaaaac ggcagaatct gaacgttatc ccatgaacga gaagatcctg
     2221 gcagtaattc ttgccctctc tctccttgcc aacctaggga tggggctgta tattgcaaac
     2281 gatcatctct acggacctgg catgctccgg aaagagtatg agactgacaa gggtgcagac
     2341 aggaccatcg gaccctccgg agaatccggg agaacgatac ccgtggccga tatccttgat
     2401 accatatcag gccaggagaa acggtctgca tccctccagg ggctggctgt catcaccacc
     2461 atcaattatt cccgccaggg acagttcatc taccaggaga agaatgagac cgggaccacg
     2521 atcaatgtca cggttgatat cattcccggc gaagggaggg ttcttgtaca gacaaagccc
     2581 ctcatgggca tcgtgttcca ggacgctgca aatactgccg tggctgttgc caggaaccgc
     2641 accactgccg acttcagtgg gagtgatgtg atcttctcaa tcacctccga gggagtgatc
     2701 cctgcagtgg acggggcgag tgccggggca ctgatgacca tcattaccag agcggcagcc
     2761 actggagaac ccctctggga gaacaggacc ctcaccggca cgataacccc ggatggccac
     2821 ataggggcta ttggcggggt gatcgagaaa gcaagagcgg caaaagtggc aggaaaagat
     2881 ctgctcctcc ttccgcggga aaacgcctac atcgtcgtac taaacgagtc ctcccggagc
     2941 atgggagggt tgcgcatgac ccggctggtc tatgaaacag tccctgcaaa agagtacatc
     3001 gaatcaacaa tggggatcag ggttgagttc gtcgatacaa tcgatgaggt ggaagcatac
     3061 ctctttcgaa caggtcaatc gggctgaccg gtaaaaatca gagagagaag gggaacaggt
     3121 cactcacctg gacgattccg ctcccatcga atggctggag ggtgaaggag acggaatttc
     3181 tcccccccaa ctttacgtca acctgttttg tctcaggctg ataaccatct tttgacaccg
     3241 ttacattgtg cgatcctgca gctatcttcg agagaaggac gttggtctgc ccgctctcca
     3301 ccccatcaat cgtgatgacc gcaccttttg gaaccgattt gacgttgagg gtcccggtgc
     3361 cggttaatgt ggtggttcca tctccggttc cgggttcaat atcccctccc ttcttgagtg
     3421 tgaatgagac ccattccacg cctcccgtgt tcacactgac ctgcttcgtc ccggggaggt
     3481 atcctggctt ggtgagggtg atggtgtgaa tgccggaagc aatgttccgg acatacatgt
     3541 tcgtctgccc gctctccacc ccatcgatcg tgatggctgc cccgctcgga atcgagctga
     3601 tatagagagt acccgttccc gttccgggtt ccccgctttc tttcagcgtg aacgccaccg
     3661 agttcatgcc acccgcctta acatcgacct gttttgtctc gggatggtat cccgacctgg
     3721 tgagggtgac gtcgtggatc ccagcggcga cgttcgagat gatagcattg gtctgtcctt
     3781 tcaccacccc atcaatagtg atcagggcac ctctcgggat ggaggtaaca tacaacgttc
     3841 ctttcccaac ggtgccacca tcccctccac tggcgatgaa cgaggcagta atggtatgat
     3901 tgcccgagat attggtaaag gtaaaggaat cagacacctc ctgattctcg ccgtccacaa
     3961 cgatagtctg gatcgtatat ccggggcttg cggtaatggt gaatgtgatt gtctctttcg
     4021 gtacgacctc aagttgtccg gagggcgcaa tcactccgcc atttccggcc gatgcattga
     4081 tgtaataccg atcatgcaat tcttcaatga ctctgaacga tgcggagatg gtg
//
LOCUS       DAOOCO010000164         4130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_20424_length_4130_cov_4.162209, whole genome shotgun sequence.
ACCESSION   DAOOCO010000164 DAOOCO010000000
VERSION     DAOOCO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20424_length_4130_cov_4.162209"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK154_08700"
     CDS             <1..350
                     /locus_tag="PK154_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11175.1"
                     /translation="WIIMLVIILIIAYFIIKEIRLMSNTRRLAEIDLEKEKLGLMQAE
                     MAQRGTPFFRVAPEKMQEIRTLDEENTALSTAIFAKHSAIDKRIERLENKVTITKLDR
                     MVNKIKDEEKKLG"
     gene            356..661
                     /locus_tag="PK154_08705"
     CDS             356..661
                     /locus_tag="PK154_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11176.1"
                     /translation="MYENAPAETAKKSTFKESLMAIPGTIDSNLPSMDKSLDRYFDAH
                     MSVIISEWGLITMHTLDDLDDRLEMVTTEIRNLERGRDEIEKRAAALDAGIRELEAQ"
     gene            658..885
                     /locus_tag="PK154_08710"
     CDS             658..885
                     /locus_tag="PK154_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11177.1"
                     /translation="MMVGMDSYMNAYLDRRMKNLIQDWDLATRNDLGDFETRLQAIEE
                     TSKDIASFETSAAIKLEELEHRLLKLREVKR"
     gene            882..1223
                     /locus_tag="PK154_08715"
     CDS             882..1223
                     /locus_tag="PK154_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11178.1"
                     /translation="MTPLEAALIIILVAVVLFVIYYYWKGTKGGLSLTRPMESRVDEY
                     LDRRFELMIDEWSLVSRARLQSFRNSKDQTLEADEAKVASLKTFSETMDQNLKQLEAR
                     LETLEKQAGMK"
     gene            complement(1324..2121)
                     /locus_tag="PK154_08720"
     CDS             complement(1324..2121)
                     /locus_tag="PK154_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW11179.1"
                     /translation="MAWEAESPTMTRLQVPGAPDGIVFIADPHIKAGNIEMIRGVIRE
                     INKMQPSLVLIGGDFYGQCEETPSFQEIWNEVDAPVYAVLGNHDYRVGIQGSGAEGRM
                     AWAMESIFRSQGYNTSTLYSNPDIASADILEDVLENNGVTVLRNEWVELDVNGQRLIL
                     CGVDDIWARRADPPVLPDNEAYRIYLIHEPYCDEGWDADLILAGHTHGGQVGNGIFMV
                     LDIVGIVDIRGRSMKDDTLMYVTRGIGTSKGDDDYRLFTSPEIVVIN"
     gene            complement(2341..3660)
                     /locus_tag="PK154_08725"
     CDS             complement(2341..3660)
                     /locus_tag="PK154_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPW11180.1"
                     /translation="MREVESSKKIEQATDSLNTRKFYFLLLFVSLLGVIGALLMVLFF
                     FLEDLLTGLIWRDIPVGSLTPVFNPWILVIGVAGGLCVGLIRYYFHGQIAIMAEDLNE
                     FNEKGRFGLKQGIELFIRGLASLVCGAPLGPEAPLTVSTGAAGTLIAEKARMPPPVVT
                     LSSLSAISGFFGAFLATPFGGALIFIEITLEKGIMTWKAILPSIIAATAGFAAYFLIA
                     GSFLEGMFTLPPFEGFRDIYLVYAIGLGLLGGGCGIAFILIYKKMREIFEPLENRPVL
                     LGMLGGLGLGIVGMLLPLTLFMGSDQLQVLVDNSLEVSLLLLLVLVLAKILLVTFSFS
                     TGFAGGYIFPGFFIGGTLGILAFRLFPFIPLEVCLVCVIAGVSVALLRSPIALAMIIA
                     MVFDPALIPAMAIALVMGFIVSYRFSLPYIRKINREQSDDNTPRDVW"
     gene            3829..>4130
                     /locus_tag="PK154_08730"
     CDS             3829..>4130
                     /locus_tag="PK154_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11181.1"
                     /translation="MNREEIGIRELILSYRDQGGGVALSSQEHHQAKGDRGKQNNPFV
                     CVPYVIYKTNLYPGFFWDENQKSLRFKNASDTDGKKEGKGGQRGRTPQTVLYFLQPG"
BASE COUNT         1122 a         1033 c         1083 g          892 t
ORIGIN      
        1 tctggatcat catgctcgtc atcatcctga ttattgcgta tttcatcatc aaggagattc
       61 gcttgatgag taatacccgg cgtcttgcag agatcgatct tgagaaggag aaactcggtc
      121 tgatgcaggc cgagatggcc cagcggggaa ccccgttctt ccgggtagct ccggagaaga
      181 tgcaagagat ccgcacgctt gatgaggaaa atactgctct ttcgaccgcc attttcgcca
      241 agcacagtgc tattgacaaa cggatcgagc ggctcgagaa caaggtgacg atcaccaaac
      301 tcgatcgtat ggtgaataag atcaaagatg aagagaagaa actcgggtga tcacaatgta
      361 tgagaacgca cctgcagaga cggccaagaa atccacattc aaagaatccc tgatggcgat
      421 tcccgggacc atcgattcta accttcccag catggacaag agcctcgacc ggtacttcga
      481 tgcccacatg tcggtgatca tcagcgagtg gggactcatc acgatgcata cgctcgatga
      541 tcttgatgac cggctggaga tggtgacgac ggagatccgg aacctggaga ggggcaggga
      601 cgagattgag aagcgtgcag ctgcccttga tgcagggatc cgcgagctgg aggcgcaatg
      661 atggttggca tggacagtta catgaacgcc tacctggacc ggaggatgaa gaacctgatc
      721 caggactggg acctcgctac ccggaatgat ctgggggatt tcgagacccg gctccaggct
      781 atcgaagaga cctcaaagga tattgcctcg tttgagacaa gtgcagcgat caagctggaa
      841 gaactcgagc accgtctcct caaattgagg gaggtgaagc gatgacccct ctcgaggcag
      901 cgttgatcat tatccttgtc gccgtagtgc tattcgtgat atactattac tggaaaggga
      961 cgaaaggagg attgtcgctc acccggccta tggagagcag ggttgacgag tatctcgacc
     1021 gcaggttcga gctgatgatc gatgagtggt cgctcgtcag ccgggccagg ctgcagtcgt
     1081 tcaggaactc caaggaccag acccttgaag ctgatgaggc aaaagtagca tcgctcaaga
     1141 ccttttcgga gacgatggat cagaatctga agcagcttga ggcccggctg gaaacactgg
     1201 aaaaacaggc aggcatgaag taaaaggaga ctgtgccttt tctcctgact gggtttaggg
     1261 ctcttgaata gcatatacct ttttttccca atctttcaaa taattcgtat tcttgcccca
     1321 cactcagttg attactacaa tctccggtga ggtaaagagg cggtaatcat cgtcaccttt
     1381 cgaggttcca ataccacgag ttacgtacat gagggtatca tccttcattg aacgtccccg
     1441 aatatcaaca atcccgacaa tatcaagaac catgaatatt ccattcccca cctgcccgcc
     1501 atgagtatga cccgccagga tgaggtcggc atcccatcct tcgtcacaat acggttcatg
     1561 gataaggtag atacgatatg cttcattatc aggcaggact ggcgggtctg ccctgcgggc
     1621 ccagatatca tctactccac acaggatgag cctctgtcca ttcacatcca gttctaccca
     1681 ttcattccgg agaactgtta caccattgtt ttcgagcacg tcttcgagta tatccgccga
     1741 agcgatatcc gggtttgagt agagcgtact ggtattgtac ccttgtgagc ggaagatcga
     1801 ttccattgcc catgccatcc ttccttccgc tcctgatccc tgaatcccga cccggtaatc
     1861 gtggttccca agaactgcat aaaccggtgc atcaacctcg ttccagatct cctggaaaga
     1921 gggcgtctct tcacactggc cataaaaatc acccccgatc agaacgaggc tgggctgcat
     1981 cttgttgatc tccctgatca cgcctcttat catttcgata tttccagcct tgatatgggg
     2041 atctgcaata aaaacgattc catccggggc accagggacc tgcaggcggg tcatggtcgg
     2101 agattcagct tcccatgcca tgtagccggg gagtacgata agtggtatga agaggagcat
     2161 gaaaacgaaa taccaggatt ctttcccacg gggaggaaga ggcatacggt ctctaatgat
     2221 tacaccggtg tttacataaa atatgagcaa aaagatccat cgtcatttca ttgggttatt
     2281 ttcgtctgaa aggaagagga tagttcctcg atccaaatct ctattcttcc ttcagcacgg
     2341 ttaccaaacg tctctgggtg tattgtcatc gctctgttct ctgttgatct tcctgatata
     2401 cgggagagag aaccgatagc tcacaatgaa acccatcaca agggcgattg ccatcgcggg
     2461 gatgagggcc gggtcaaata ccatggcaat gatcattgca agggcgatag ggctcctgag
     2521 caatgcgaca ctcacgccgg caatgacgca gaccaggcag acctcaagag gaatgaacgg
     2581 gaagagccgg aaggcaagga tcccgagagt ccccccgatg aagaacccgg gaaagatgta
     2641 tcctcctgca aagccggttg aaaatgagaa ggtcacgagg agtatctttg caaggaccag
     2701 gacaagcaga aggaggaggc tcacctcaag ggaattatct acgagcacct ggagctggtc
     2761 acttcccata aaaagggtga gagggaggag catccccacg atcccgagac ccagaccccc
     2821 cagcatgccc aggagtaccg gccggttctc cagcggctcg aaaatctctc tcatcttttt
     2881 gtatatcagg atgaatgcaa tcccacatcc acccccgaga aggccaagcc cgatggcata
     2941 gacgaggtag atatcccgga atccctcaaa cggggggagg gtgaacattc cctccagaaa
     3001 agagcctgcg atgaggaaat aggctgcaaa tccggccgtg gctgcaatta tggaggggag
     3061 gattgccttc caggtcatga tgcccttttc cagagttatc tcaatgaaga tgagggcacc
     3121 cccaaacggg gtcgccagga atgcaccgaa gaatccgctg atggcagaga gggaggagag
     3181 ggtgaccacg ggcggaggca tccgggcttt ctctgcgatc aacgtccctg ccgctccggt
     3241 ggagaccgtc aggggagctt caggtccgag gggagcccca cagacaagtg aggccagtcc
     3301 gcggatgaag agctcgatcc cctgtttcag gccaaacctc cctttctcat tgaattcgtt
     3361 caggtcttcg gccatgatgg cgatctgacc atggaaataa tagcgtatca gcccaacaca
     3421 gagccccccg gctaccccta tgaccaggat ccagggattg aagacaggcg tcagggaccc
     3481 aacaggaata tccctccaga tcaggccggt gaggagatcc tcgaggaaaa agaagagcac
     3541 cattaacagg gcaccaatga cacccaaaag cgagacgaag aggagcagga aataaaattt
     3601 ccgggtattc aatgaatcgg ttgcctgctc gatctttttg ctgctctcaa cctctctcat
     3661 ggtgacgtcc ttatcagagt tgaaaacaca gggactatta aatgtcttcg ctttgtgtta
     3721 ccggtattga tacccctcgg tgaaaaagga atatggtcag aacatgttta ccctccaagg
     3781 cttctgctat ggtgagggtc aaacacaaag gaaaaagcca ggaacttatt gaacagagag
     3841 gaaatcggga taagggagtt gattctatcg taccgagatc aaggaggggg agtcgccctc
     3901 tccagccagg agcatcacca agcgaaagga gaccgcggga aacagaataa tccttttgta
     3961 tgcgttcctt acgttatata taaaactaat ctatatccgg gcttcttttg ggatgaaaac
     4021 caaaaatctt taagattcaa gaatgcatca gatactgatg gcaaaaaaga aggaaaaggc
     4081 ggtcaaagag gaagaacccc tcaaactgtt ctatattttt tacaaccagg
//
LOCUS       DAOOCO010000165         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_20687_length_4092_cov_2.859797, whole genome shotgun sequence.
ACCESSION   DAOOCO010000165 DAOOCO010000000
VERSION     DAOOCO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20687_length_4092_cov_2.859797"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(909..3656)
                     /locus_tag="PK154_08735"
     CDS             complement(909..3656)
                     /locus_tag="PK154_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11182.1"
                     /translation="MPAMADIIVYSHGFEADDGGYTHTGTLDQWEWGTPAFAAGPVSA
                     HSGLRVWGTNLDGNVPYSSDSYLTSPGIAIPAVTSDQIIRVRFWAWIAVDEMLDRGEF
                     QVSSDGISWETKSELFHVMQGDWSEYYFDITDYADGSIYLRYRLYADGGDEFDQSPYN
                     MSGLYIDDVAIIISDKPALQTTLILEAYEDDYYLASCPWIYSWNGGEFEKDNDVYPTS
                     RGPAKEVTDYYTLNTPVVSEDGHYIFKLQETDSESSYTDVVQLITVDHSTMVKISADD
                     GGTIWTYGSPSAPASASTSAGNDAVAALSVEDERGLNVGNGDYVELDFNNLDITNGAI
                     LVLRVLGYQNDGPVGDPTYVTPAIAIQTQDSDEQWVTRHRFAPRMDWATSAYNLKDDF
                     VINNKVRLYATSCHEGKYHIIDYVGMDTSYQAEVSVTTVPLASATHSVNGDVCAALQV
                     SDNNYATMTSLESLELAFAVPAQSGEARDFVFVSEGYYVPIGTYFIYTWDGSQWAQRD
                     GWSIEGGVDATRSFDLSMWLPDPSGEYKVRIWQDYWYENAGIDYVGMTQGLVTGTMIS
                     AFDLRKAIDVTALLISSDNTRDEWLYDDAGYPYSRSRNRWVEVKWTGLSINTPPTTNP
                     VLIDNPLLATPTISWTYADVDGDSQVQFEVEVWTGTGGTGTNVWDPAIGTGDDSSIVY
                     AGSPLVIGSTYYARVRAFDGTSWGGWSETSWTYELPPFTVEKDFRYTAVDFTPYNCGL
                     DGICRTDDDYQEPADLGMTLPDTDGDGMYEVKYVVNKKGEVTSTNPGQLYAVITITGI
                     GIDTVALDDAFGTQFDVNPGKLGGGVEVLKIGVDGLAEVLTDTPQVTFASVDNDAGEV
                     ILAIDLDTPLEIDEELMIYLKYQTALKFEMPDTTDFTNTADVTINELTGITAVAVVEF
                     V"
BASE COUNT         1055 a         1079 c          909 g         1049 t
ORIGIN      
        1 tttccagtgc cttgcccgga ttttttctac ggtcaccgcg aactacccct gccccctgtg
       61 catgtgatag tatgtgtgct ttgtttcagt catgtaaatg gttgtggtcc agccagagag
      121 ccactccgtt ctgatcagcg ggtccggtca tgctgagctg cccattatcg atcgtttcca
      181 tgccgccttg taacgctcgc caggggaggg atttggctcc ttgctcaaac ctcctctatc
      241 cgacaagaaa ccatccatat ccatcactcc tccttttggg tcaagcagcc tacctgcaca
      301 gggagagatt gaacctgaac tatcgtagta tttcttatat gctggctttg agatgccttg
      361 gctggtaagt cagggaaaaa tccctgattg cccggaggcg aatcagcctc gtcccggtac
      421 tgcctcaccg gtacgatttc caggggatac cgccttcgta acagctatgg aagtgcagaa
      481 gagatcagtc acattcgatg cccgatcaga gtgtcgaaaa aatgtagggg aatagcgcat
      541 acgttgttca aaaaccggag cacacgctgg tctgaacaat ccgaatccgc atattcatgc
      601 agtcacgaat aaccgctgga gggtaagaat atcccatacg atagccggtg gaagccctcc
      661 ccaacggcat tatttttccg gcgtttcagg gttttttaaa aaagactgtt ctgttcgatg
      721 ctcgaacctt tgcaggagtg gggctggtac cgggagttca gttctgagac ttccctgtca
      781 agtcattctt accccgattt ttatcccttc ctccttggga aacctcgtgg aattaccgac
      841 actacctcgt ggaactacct gctctaagat gcgaagatag ttcggaaaaa aagaatgcct
      901 tgagattctt acacaaattc caccactgcc accgcagtta taccagtgag ttcattgata
      961 gtgacgtcgg cagtgttcgt gaaatccgtg gtgtcaggca tttcgaactt cagggcagtc
     1021 tggtacttga ggtagatcat cagctcctcg tctatctcca acggcgtatc gagatcaatt
     1081 gcaagaatga cttcccctgc atcgttgtca accgaagcaa aggtcacctg cggggtatcc
     1141 gtaagtactt cggcaaggcc atccacgcct atctttaata cctcgactcc tccgcccagc
     1201 ttgccgggat tcacatcgaa ctgcgtcccg aacgcatcat caagggcgac ggtatcaatg
     1261 ccaatccctg tgatagtaat gacggcatag agctggccgg gattggtgct cgtcacttca
     1321 ccttttttat ttaccacata cttgacttcg tacatcccat cgccatcggt atccggaagc
     1381 gtcatgccaa ggtcagccgg ctcctggtag tcatcatcgg tacggcagat accgtcaagt
     1441 ccacagttat acggcgtgaa atcgactgcc gtgtacctga aatccttctc gaccgtgaag
     1501 ggaggcagtt cataggtcca tgatgtttct gaccagccac cccagctcgt accatcaaat
     1561 gcccggaccc gggcataata cgtggatcca ataaccaggg gtgatccggc atatactatt
     1621 gaactgtcat ccccggtgcc aatggctgga tcccagacat tggtcccagt ccctccggtt
     1681 cctgtccaca cctctacctc aaactgcacc tggctatcac cgtctacatc tgcatacgtc
     1741 caacttattg ttggagtagc aaggagtgga ttatcgataa gaacaggatt tgttgtggga
     1801 ggcgtgttga tcgaaagccc tgtccatttt acttcaaccc aacgatttcg ggaccgtgag
     1861 tagggatacc ctgcatcatc atacaaccat tcatcccggg tattatctga agagattaac
     1921 aaagccgtaa catctattgc tttccggagg tcaaacgcag aaatcatggt gccagttacc
     1981 agtccttgcg tcatcccgac atagtcgatt cccgcatttt cataccaata atcttgccag
     2041 attctaacct tatactctcc cgaggggtcc ggaagccaca tactcagatc gaaacttctt
     2101 gttgcgtcaa ctcccccttc gatcgaccaa ccgtccctct gtgcccattg gcttccatcc
     2161 caggtgtata tgaagtaagt ccctatcggc acatagtatc cctctgatac aaagacgaaa
     2221 tccctcgcct cacccgattg agcaggcaca gcaaacgcca gttcgaggga ctcgagcgag
     2281 gtcatcgtag cgtaattatt gtcagaaacc tggagggctg cacagacatc gccattcaca
     2341 gaatgcgtgg ccgatgccaa tgggacagtg gtaactgaaa cctctgcctg ataagaggtg
     2401 tccatgccga cgtaatcaat aatgtggtat tttccttcat ggcatgatgt ggcatataat
     2461 ctgaccttgt tattgatgac aaagtcatct ttcagattat aggcactggt tgcccagtcc
     2521 attcttggtg caaaccggtg cctggtaacc cactgctcgt cactatcctg ggtctggata
     2581 gcaattgctg gtgtgacgta ggttggatct ccaacaggcc cgtcgttctg gtagccaagc
     2641 acccgcagaa cgagaattgc cccattggtt atgtccaggt tattgaaatc gagttcgaca
     2701 tagtcaccat ttccaacatt caatcccctc tcatcctcaa cggagagtgc agccacggca
     2761 tcgttaccag ctgacgtgga tgcagaggcc ggagcactgg gacttccata agtccagatg
     2821 gttcctccat catcagctga gatcttgacc atggttgaat gatcaactgt tatcagctgt
     2881 actacatccg tatatgacga ttcagaatct gtctcctgga gtttgaatat ataatgaccg
     2941 tcttcggaaa ccacaggtgt gttcagggtg taataatcgg tgacctcttt cgccggacct
     3001 ctggaagtag ggtaaacatc gttatccttt tcaaattccc ccccattcca agaatagatc
     3061 caaggacatg atgccaggta ataatcatcc tcatacgctt caagtataag ggtggtttgc
     3121 agtgctggtt tatctgaaat gatgatggca acatcatcga tatacagacc tgacatgtta
     3181 tagggactct gatcaaattc atctcctcca tccgcataca atcgatatcg gagatatatc
     3241 gaaccatccg cataatcagt gatatcaaag taatattctg accagtcacc ttgcatgaca
     3301 tggaacagtt cactctttgt ttcccatgag atcccatcgc tggaaacctg gaactcacct
     3361 ctgtcaagca tttcatcgac tgcgatccag gcccagaaac gaactcggat aatctgatcg
     3421 ctggttactg ccggtatcgc aatacctggc gaggtaagat acgaatcact cgaataagga
     3481 acattcccat caagattggt accccacacc cgtaaccctg aatgagcaga tacaggcccc
     3541 gctgcaaatg caggagtacc ccactcccat tgatcgagcg ttccagtatg ggtgtatccc
     3601 ccatcatccg cttcaaatcc atgggaataa acaatgatat cagccattgc tggcatcact
     3661 gtgaaaatgg aaagaacgag aagtatgaat ggaattacct tccaataatc agctgcttgc
     3721 tgaaccttca taagaccatc tcctctattg tgagcatatc agtcatattg caatgcgaag
     3781 attcttaaaa aaaaatgcaa agaaccgggg taattgttac gtgacgtaac aggtataaga
     3841 ggtaagattg atcagcacca ccccccctcg gctttgatag ggaaatctat catccagtat
     3901 ttattacttt ctctattaaa gtagagatac acttgcctgg ttctgtcgaa aaagggcttt
     3961 atgcacaccc caatgtcgaa acgatccagc cttgagcgag atacccgact cactctaaga
     4021 cttcagcagt gggcagtctc cctgaaaaag agaaaccatt tgacaccggc cattaatact
     4081 ctggtatgaa ag
//
LOCUS       DAOOCO010000166         3995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21398_length_3995_cov_5.057360, whole genome shotgun sequence.
ACCESSION   DAOOCO010000166 DAOOCO010000000
VERSION     DAOOCO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21398_length_3995_cov_5.057360"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..593)
                     /locus_tag="PK154_08740"
     CDS             complement(387..593)
                     /locus_tag="PK154_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11183.1"
                     /translation="MIETGNYTFWYKHGECWDAANTTFRVVNGAWRMDDILPYDEDTV
                     GWTIWTSPVDEGNFTGIPVSPDLV"
     gene            complement(590..1300)
                     /locus_tag="PK154_08745"
     CDS             complement(590..1300)
                     /locus_tag="PK154_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HPW11184.1"
                     /translation="MYEDLNANERIDYNDVVVFFKNMTWIADNEPVACFDFNGNRRID
                     YNDIVRLFKEVGVPLPWDGCERYDPAANGSTVQIPLGAGGLVITLPENPSTGYHWNAT
                     VTSGLAIVDDRFIPNAQTLGVPGAGGTRVWTLSGTSEGVQTFSAIYKQPWMNVTGTEQ
                     TFTLHILVGENTSPCISLPTGTSLLSESMQGSRNLTIDNQNDDDAVVSLRIEAIPYAS
                     GSKVVSSMYGDMTSIPVR"
     gene            1875..2603
                     /locus_tag="PK154_08750"
     CDS             1875..2603
                     /locus_tag="PK154_08750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW11185.1"
                     /translation="MTDQVQRDARAWFADWANEYDTTLGKVRRHHAMLDLVVSCSGVR
                     DGDKVLDIGCGTGLLSLKFLEQADCSITAIDSSEKMLGIFREKIEQCHLSHRIHCEEC
                     SAEEMAFPPHHFDIVASTVALHHVKEKGPVMLKVYDALKEGGRFVLGDMDLDTTGDLD
                     DPRRLVRILEFLEKELALAMEDGGIPAFERMYDNGKKHILNDGEYCIGFDHWEQLCKD
                     AGFLQVEVTPVEGFEWFKVLVARK"
     gene            complement(2671..3822)
                     /locus_tag="PK154_08755"
     CDS             complement(2671..3822)
                     /locus_tag="PK154_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPW11186.1"
                     /translation="MTVPSVHPIRLLFIDNIRILLVCLVILTHLAITYGASGSWYYHE
                     VSGLSVSSALLTLLASLFQTFFMGFFFLIAAYFIPSSLKRKGMYRFVSDRLARLGVPL
                     VIWILFISPIFGYLVARGLEGFPGAFRDWYYTVQPNLHYLGLGPMWFVFSLLVFTLVY
                     VAWNAIRRTSEHEVASPRPVPTIPLILLFGIFLALVSFGVRIIFPIGYEWELFALQVP
                     YYPQYIALFIIGIIAAGNDWFVRVPDSTGKTCSFFALLLALLQPLLLLTVDLSSGDLT
                     PFFGGLHWQALAYALWEQVTGMLIIVSLSWVFYTRFNHQGSVARSAAADSYTVYLIHP
                     LVLVPLAVSLQAVVLLPFLKFLAVSALAVPLCFVLAHLLRAIPGAKRIL"
BASE COUNT         1020 a          994 c         1146 g          835 t
ORIGIN      
        1 agcaatttcg cctggttcaa tgctcaccgt actggttgcc gttacgggta taaccagcag
       61 gatgagaaag atgatactat acaatagcca tgagtatttc atgatgccac caccttttta
      121 tagtacaagt cttgacggaa ggggtataaa aatgtatggg ctgattttcc ggttctgttc
      181 gacgtttggt ccaattccta cggaaaaaaa cacactaata cctggacgct ggtatcaggt
      241 aattcgctga ctcggggcgg gaaatagtcc tcctcaatca gatcacataa aaaaggggta
      301 ctgcaatcgt atctcccatt gatgatggaa aacagtcctc ctcaaccagt gacgcacgaa
      361 aaaaggggaa ctactatcct gtctccctag acgagatcgg gggaaacggg tatgccagta
      421 aaattgcctt cgtctaccgg ggaggtccag atcgtccagc cgacagtatc ctcatcatag
      481 gggaggatgt cgtccatcct ccatgccccg ttgacgaccc tgaatgtggt gtttgccgca
      541 tcccagcatt ccccatgttt gtaccagaac gtgtagttcc cggtttcaat catcgaacag
      601 gtatactggt catgtccccg tacatagaac tcaccacttt gctcccggat gcatagggta
      661 ttgcctcgat gcgcagggat accacggcat catcatcatt ctggttatcg atcgtcaggt
      721 tccgtgatcc ctgcatggat tccgacaaaa gggaggtgcc ggtcggcagg ctgatgcagg
      781 gagaggtgtt ctccccaaca aggatatgca gggtaaaggt ctgttcggtg ccggtcacat
      841 tcatccaggg ctgtttgtag atcgctgaaa acgtctggac tccttccgag gttccagaga
      901 gggtccatac ccgggtcccc cctgcaccgg gaacgccaag ggtctgtgca ttgggaataa
      961 accgatcatc tacgattgcg aggcccgagg tcacggttgc attccagtga tacccggtcg
     1021 aaggattctc agggagcgtg atgacaagcc cccctgcacc gagcggaatc tgcacggtgg
     1081 agccgttggc agccgggtca tacctctcgc acccgtccca gggcagcggc actcccactt
     1141 ccttgaagag cctcactatg tcgttgtagt cgatccttcg attcccgttg aagtcaaaac
     1201 aggcaaccgg ttcattgtca gcgatccacg tcatgttctt gaagaagacc acaacgtcat
     1261 tgtagtcaat ccgttcattg gcgttcagat cttcatacaa gccgtcaaaa tcgggatcgg
     1321 tcggtgccag aaaataccct ggcacagcaa ccggggttgc agccagtgac ggaagagtca
     1381 gtgctgtaca taatatgaga gagacgaata gtcaccttga ttgaatgata taccaccttg
     1441 aataatagaa aatagccctg ttctataaaa aggttgggga ggcagaggac gcttttcctc
     1501 ctccttcctc ccaagctatc ccttcccgga tggaaaaatg gggagtgggg gagggaacac
     1561 ctggtgagcc ttttcttggt tcctcccaga ggggcgggac gataaccgta cggcaccatc
     1621 ctcccggtga ggatcgctcc gggtgttcct tcccggggat cgtcctggga gatctcttcc
     1681 cggaggtcgc cgcaatggag cgtctcgtca tcgggcatgg gagatatcgg gtatttcaaa
     1741 ccgaacgaac atctctcttt ttcctcatgg ccttcccttg ccattcgtaa gaattccggg
     1801 agagcacccc catgccgaag aaggctgact ccgcacgaca acgtaatatc tctcccctcc
     1861 aatagactga cacaatgact gatcaggtgc agagggatgc ccgggcatgg tttgccgact
     1921 gggcaaacga gtacgacaca acacttggca aggtccggcg gcaccatgcc atgcttgacc
     1981 tggtagtgag ctgctcagga gtccgggacg gcgacaaggt ccttgacatc ggctgtggga
     2041 cgggactgct gtcgttaaaa ttcctggaac aggcagactg cagtatcacg gcaatcgaca
     2101 gctctgagaa gatgctcggg atattccggg agaagatcga gcagtgccat ctctcccaca
     2161 ggattcactg tgaagaatgc tctgctgagg agatggcgtt tcctccacat cacttcgata
     2221 tcgtcgcgtc aacggttgca ctgcatcacg tgaaagagaa aggtcctgtg atgctcaagg
     2281 tgtatgacgc tctgaaagag ggtggacggt ttgtcctggg cgatatggat cttgatacga
     2341 caggggacct ggatgatccc aggcgtctgg tgaggatcct tgagtttctc gaaaaggaac
     2401 ttgcccttgc catggaggat gggggcatcc cggcatttga acggatgtat gacaacggga
     2461 agaaacatat cctgaacgac ggggaatact gcatcgggtt tgaccactgg gaacagctct
     2521 gcaaggatgc cgggtttctg caggtggaag tcacccccgt agaaggattt gaatggttca
     2581 aggtgctggt ggcaaggaag tagggggagg aggagaagag aagaggtact actctccgct
     2641 gtcggttgac tttttctgca ctcttttttg ttagaggatt cgttttgctc ccggaatcgc
     2701 acgaaggaga tgggcaagaa caaagcagag cgggacggca agggcactta cggcgagaaa
     2761 cttgagaaaa ggcagcagga ccactgcctg aaggcttacg gcgaggggga caaggacaag
     2821 cgggtggatg aggtagacgg tgtaggaatc tgcagctgca gatcgtgcga ccgagccctg
     2881 gtggttgaac cgggtgtaaa acacccatga gaggctgacg ataatcagca taccagtcac
     2941 ctgctcccac agcgcgtacg caagcgcctg ccagtgaagc cccccgaaga acggggtgag
     3001 atcgccggat gagagatcaa cggtcaacag aagcaggggc tggagcaatg ccaggagcag
     3061 ggcgaagaaa gagcaggttt ttccggtgga atccggaacc cggacgaacc agtcattccc
     3121 tgctgctatg ataccgatga tgaagagtgc gatatactgg ggataatacg gaacctggag
     3181 agcgaaaagt tcccattcgt atccaatcgg gaatatgata cggacgccga aactcaccag
     3241 ggccaggaat attccaaaga ggagaataag gggtatcgtc gggacgggcc ggggactggc
     3301 cacttcatgt tccgacgttc tcctgatagc gttccaggcg acgtagacca gggtaaatac
     3361 cagcagagag aagacgaacc acatagggcc caacccaagg tagtggaggt tcggctggac
     3421 cgtgtaatac cagtccctga atgcaccggg aaatccttca agccccctgg caacgaggta
     3481 cccgaagatc ggtgagatga acagtatcca tatgaccagg ggaactccca gtcgtgccag
     3541 ccggtcagag acgaaacgat acataccctt ccgcttcagg gaggagggga tgaaataggc
     3601 ggcaatcagg aagaaaaaac ccatgaagaa ggtctggaag agcgaagcaa gcaaggtgag
     3661 cagcgccgat gataccgata acccagagac ctcatggtaa taccaggaac cggaggcgcc
     3721 gtacgtgatc gcaagatgtg tcaggatgac caggcagacg agtaggatgc ggatgttgtc
     3781 gatgaaaagg aggcggatcg ggtgtacgga gggaacggtc atcgtatagc ctggtatcgc
     3841 tcatcattgt gatggccgtg agaggggaaa accatagtgc agacccatgt tcccgtctca
     3901 aagaatctct ccctgtacca tccgatgaaa tgttccatgt ctgatcgcac ctctgcacca
     3961 tcaaccgatg acaccgagta ttgccgcaaa ggcaa
//
LOCUS       DAOOCO010000167         3995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21404_length_3995_cov_3.913706, whole genome shotgun sequence.
ACCESSION   DAOOCO010000167 DAOOCO010000000
VERSION     DAOOCO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21404_length_3995_cov_3.913706"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..495)
                     /locus_tag="PK154_08760"
     CDS             complement(61..495)
                     /locus_tag="PK154_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW11187.1"
                     /translation="MQVIADFQFGQGAGLALFPEGCEAILSRTGRVRQISLGGNRLAT
                     VRAMDGRLTLGIEGALRLLSCLAPPAYRVTVAADVAEFVRQGKNTFAKHVIAADPGIR
                     AGDEVMVVDDQDQLLATGSALLSGEEMLFFNYGVAVKVRQGR"
     gene            complement(479..1876)
                     /locus_tag="PK154_08765"
     CDS             complement(479..1876)
                     /locus_tag="PK154_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPW11188.1"
                     /translation="MGLSEDVRAVVWDLLSQESVTIISHIDADGITGEAILSQALSRA
                     GIRTTSVFIRQLEPLTIRQVPKDETFKLFCDLGAGQQNLIAEAGFRNDEVGIIDHHIS
                     QPCDVDYLQVNCHPYGHVKLSAAGVAYLVAKEIDSGNSDLAPLAVIGNIGDMMAREDC
                     GLTGIAREIAYEGSAHGSVEVRKHDLNCYGISTRPLHISLSYNDDPIIPGISNNARGA
                     LRFLQKLGIPLQDNLQRWKVWEEFSCEEQRIISSALAQQLLAHACSITRLFCESYLFP
                     AERARTPLRNAQEYATLLNACGRWSRPKTGAQICRGDRGSAYREAEHMLTNHRTIIRE
                     LLQFILDTGVSELSHLQYLHVGNRYPDTIVGIGAGMALSKLNREKPILIMVEQPEDPS
                     LTKVSLRANEQMLSRGIDLQEAATSASERFGGAGGGHRIAAGAFIPRESEHEFIQYVN
                     ELLAGQCTKEDAGHR"
     gene            1941..3437
                     /locus_tag="PK154_08770"
     CDS             1941..3437
                     /locus_tag="PK154_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPW11189.1"
                     /translation="MYRSSSLSYGIYFITTHNLTIKEGECRVQQKYGHPVAGVAGFAE
                     TGIITPERMQGVDANAQALGLSSLQMMESAGRALADAVRARHSKRVLILCGKGNNGGD
                     GMVAARYLQDLHPMVFVIAGSGMTRESAEQLRLIHYCNVEIVPVLCRDDVIAQADHFS
                     RSDLIVDALLGTGLRGAVKEPVRSCVEMANQSSAMILSADIPTPGIRADYILAFHRPK
                     VAGSAVAQIGIPLEAELFTGPGDLLTIRKKRPDAHKGSGGEVLVIGGGPYQGAPYLCG
                     LGALRAGADIVRIASPVFEPVPELIYERLEGDIIGAEHIDRILPLIDRADVVVMGNGL
                     GSRSHHVVRALAPSCRRVVFDADAIRLPIPEAGDSIFTPHAGEFERMTGGKPGDDLVT
                     RAHAVRAAAKRGIVILKGPIDIISDGSRVRFNRTGTPAMTTGGTGDVLAGVIAGLFCQ
                     MPGFEAACAGAYATGKAGMMIGAIHEGGMLASDLLSYIPKILFGKGGG"
     gene            3437..3901
                     /gene="moaC"
                     /locus_tag="PK154_08775"
     CDS             3437..3901
                     /gene="moaC"
                     /locus_tag="PK154_08775"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPW11190.1"
                     /translation="MAEFTHISDDRVRMVDVGEKAEMKRLAVAAGSIYLRPETLVAIR
                     DGTMIKGNVLATARVAATLAVKDTFRIIPMCHAIPITSVEVDFGQMEDHIKVTVLVRS
                     IGKTGVEMEALTGVSVALLTIWDMVKSAEKDAFGQYPCTRIDAIRVIQKQKE"
BASE COUNT          917 a         1016 c         1102 g          960 t
ORIGIN      
        1 cgatctgttt catcatctgc ttcatttttt tgggatttat gtttcctggc atcatgcctc
       61 tcaccttccc tgccgtactt ttactgctac accgtaatta aagaacagca tctcctctcc
      121 tgaaagaagt gccgaaccgg tcgcaaggag ctgatcctgg tcgtctacaa ccatcacttc
      181 atcccccgcc cggatgccag gatctgcggc gatcacatgc tttgcaaatg tgtttttgcc
      241 ctgtcgtaca aattctgcaa catcagcagc gacagtcaca cggtatgccg gtggagcgag
      301 gcaggagagc agccgtaaag ctccctcaat tcccaacgta agacgaccgt ccatcgcccg
      361 gacggttgca agtcgattcc ccccgaggga gatctgtcgc acccttccgg tccttgacag
      421 aatcgcctca cacccttccg ggaaaagggc aagtccagcc ccctgcccga actggaaatc
      481 agcgatgacc tgcatcctcc ttcgtgcact gtcctgcaag caactcgttg acatattgga
      541 taaattcatg ttctgactca cgcgggataa atgcacctgc agcgatccgg tgccctccgc
      601 cagctccacc gaatcgttcc gaggctgaag tcgctgcttc ctgaagatca atcccccgtg
      661 acagcatctg ttcattggct cggagggata ccttggtaag agagggatct tccggttgtt
      721 ccaccatgat gaggatgggt ttttcccggt tgagctttga aagagccatg ccggcaccga
      781 ttccgacaat ggtatcaggg tagcggtttc cgacatggag atactggaga tgtgagagct
      841 cggacacgcc agtgtccaga atgaattgaa ggagttccct gataatcgtc ctgtggttgg
      901 taagcatatg ttcagcttcc cgatatgcag atccgcgatc gcccctacag atctgagcac
      961 ctgttttagg cctcgaccac ctgccgcatg cattcagaag cgtggcatat tcctgtgcat
     1021 tccggagcgg tgtccgggcc ctctcagccg gaaacaagta tgattcacag aaaagccgtg
     1081 tgatcgaaca ggcgtgtgca agcagttgct gagcaagggc gctggagatg atccgctgct
     1141 cctcacagga gaattcttcc cagaccttcc atcgctggag attgtcctgt agcggtattc
     1201 caagtttctg aagaaaccgg agggctcctc tcgcattatt gctgataccc gggatgatag
     1261 gatcatcatt gtaggaaaga ctgatatgaa gtggccgggt agagatccca taacaattca
     1321 ggtcatgctt tcggacctcc acagagccgt gtgctgatcc ttcatatgcg atctctctcg
     1381 caatcccggt aagtccgcaa tcctctctgg ccatcatatc gcctatattg cctattacgg
     1441 ccaatggtgc aaggtcggaa ttccctgaat cgatctcttt tgcaaccaga taagcgacac
     1501 cggccgcact cagctttaca tgaccgtaag ggtggcagtt tacctgcaga taatcgacat
     1561 cgcatggctg gctgatatgg tgatcgatga ttccaacctc atcgttccta aaacccgctt
     1621 cagcgatgag attctgctgg cctgcaccga gatcacagaa aagtttgaac gtttcatctt
     1681 ttggcacctg ccggattgtg aggggttcga gctgccggat aaagaccgat gttgtcctta
     1741 ttcctgcccg ggaaagagcc tgcgagagga ttgcctcgcc cgtgatgcca tcagcatcaa
     1801 tatgggaaat aatggttacc gactcctgcg aaagaagatc ccatacaaca gcccgcacat
     1861 cttctgacag tcccatcact ctatgtatcg gtagattcag gaaaaaaagg ttattttccc
     1921 ggagatcagg gtgaccaggg atgtaccgat cctcatcgct gtcttacggc atatatttca
     1981 taactaccca caaccttaca atcaaggaag gagaatgccg ggtgcaacaa aaatacgggc
     2041 atcctgttgc cggagtcgcc gggtttgcag aaactgggat catcacccct gagcggatgc
     2101 agggggttga tgccaatgcc caggcgctgg ggctttcatc cctccagatg atggagagtg
     2161 ccggaagggc tcttgctgac gcggtgaggg cccggcattc caaaagagta cttattctct
     2221 gcgggaaggg aaacaacggg ggggatggga tggtagcagc ccgttatctc caggatcttc
     2281 atcccatggt cttcgtcatc gccgggtcgg gtatgaccag agagagcgca gagcagttgc
     2341 gtctcatcca ctattgcaac gtggaaattg ttccagtttt atgtagggat gatgtcattg
     2401 cacaggcaga tcacttctct cgctcagacc taatcgtcga tgccctgctt ggtactggtc
     2461 tgcggggtgc cgtgaaagaa ccggtccgct cctgtgtgga gatggcaaac cagtcatctg
     2521 cgatgattct ctctgccgat atccctactc cgggtatcag ggctgattat atccttgcgt
     2581 ttcatcgacc gaaagttgcg ggatctgctg tcgctcagat agggattccg cttgaggcgg
     2641 aactctttac agggcccggg gacctcctca ctatcaggaa aaagcgacct gatgcacaca
     2701 agggaagcgg aggagaggtc ctcgtgattg ggggtggacc gtaccaggga gccccctacc
     2761 tctgtggcct cggggccctc agggcaggag ccgatattgt caggattgcc tccccggtat
     2821 ttgagccggt gccagaactc atctatgaac gtctcgaagg ggatatcatc ggtgcagagc
     2881 atatcgaccg tattcttccc ctgattgatc gtgctgacgt cgtggtaatg gggaatggtc
     2941 tcggcagcag gagtcaccat gtggtgagag cacttgcacc ctcctgcagg agagtggtct
     3001 tcgatgcaga tgccatccgg ttgccgatac cagaggcagg ggacagtatc tttacccctc
     3061 atgcgggtga attcgaaagg atgactggtg gaaagccagg cgatgatctg gttaccaggg
     3121 cgcatgctgt cagggcggca gcaaagaggg gcatcgtgat tttaaaagga ccgatcgata
     3181 tcatctccga tggatcaagg gtccggttca accggaccgg caccccggca atgaccactg
     3241 gtggaaccgg tgatgtgctt gctggagtta tagcgggcct tttctgccag atgccgggct
     3301 ttgaagctgc ttgcgcgggg gcgtatgcaa ccggaaaggc agggatgatg ataggagcaa
     3361 tccatgaggg gggtatgctt gcaagcgacc tgctttcgta tatcccgaaa atattatttg
     3421 gcaaaggagg aggatgatgg cagagtttac ccacataagt gatgacaggg tccggatggt
     3481 ggatgtcggt gaaaaggccg aaatgaagcg tcttgctgtt gcagccggca gtatatatct
     3541 gagaccagag acgcttgtcg caatcaggga tggtactatg atcaagggca atgtgctggc
     3601 tacagcaagg gttgcagcaa ctcttgcagt aaaggacaca ttccgtatta tacccatgtg
     3661 ccatgcaatt cccatcactt ccgttgaggt tgatttcggg cagatggaag atcacatcaa
     3721 ggtcaccgtt ctcgttcgtt cgataggaaa gacaggtgtg gagatggaag ctctcaccgg
     3781 cgtatccgtt gcgcttctca ccatctggga tatggtgaag tctgcagaga aggatgcgtt
     3841 cgggcagtat ccctgtaccc ggatagatgc tatccgcgtg attcagaaac agaaggagta
     3901 attctggttt gtatgcggtt tatgagcgca tggctttatg tggttttata atgatttatt
     3961 atggtctgcg ggatccttcc cgtaaaggaa tgtgg
//
LOCUS       DAOOCO010000168         3981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21511_length_3981_cov_5.841569, whole genome shotgun sequence.
ACCESSION   DAOOCO010000168 DAOOCO010000000
VERSION     DAOOCO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21511_length_3981_cov_5.841569"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1217
                     /locus_tag="PK154_08780"
     CDS             24..1217
                     /locus_tag="PK154_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW11191.1"
                     /translation="MEQSTLNLNDSRRARTLFILYLTGFTTMLGIGIITPVLPIYAQM
                     IGATGFWIGAIFSVFALSRTIFMPFVGRLSDAGGRRRFILLGLAGYAFFSALYVPADN
                     VLILTIVRFFHGMAAAMVYPIANAYVADMARVGEEGKTMGGFQSAAFLGMSFGPLLSG
                     IMVDLLYVQSAFIALAGLSLTALIICIAYLPEYHVPMREIPPLRSVFFSRGMRVPIIF
                     FFTYTAGYTAFMVFFPVLAHADQMTVSGIGALIFISSISTAVFQRLSGRYADLFDRHL
                     VLSTGAGVMAVSLLLFTMSEGFLSFLLTSVLLGAGIGISLTTVSAMAVIEGRNLGQGS
                     VEGVINTAQGIGIVISPLVLGLVMDFTGISMVFLTAAGVSGLMALAMMRFVYRETPAQ
                     ARNAE"
     gene            1352..2650
                     /locus_tag="PK154_08785"
     CDS             1352..2650
                     /locus_tag="PK154_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11192.1"
                     /translation="MNIPETAELIVPIAIRNVPIVTGMVEQSATIFGLDKEGAQNLGL
                     AAEEVFNVLASAARPDEKIRIVVRQGGYYVEVACYFSRHAIPITALNITKIPDPDDES
                     DIENLGIILAAKVVDRLQLVRSRQGEMALRFTIERRYPEQVIPSGIPPAGHYSVGEGG
                     FEEIKQFASRVSGMYGANSPGFFRFPGKIVDMVKSREFGVLLALDGKGNVGGGMLWKW
                     GGKMIDSFGPYIFTPQPGLVGEIVQGVLEKVGRKGAVCIVNRMPADDLLRNFFEELVE
                     SCPANRIRTTACHPPLYRQLEEDTGSVAYVHPEVEIFVKECYERLVLPRSVVATTHAG
                     EFIEPHAAFGVQVDKKQNLAVISTIVAGIDAEEVLAGYVASLYDEGITRLEFEIDLGD
                     ETTPVLVPALHAVGFIPCYVLPWGGRGDILVFECQGPRGF"
     gene            2652..3767
                     /locus_tag="PK154_08790"
     CDS             2652..3767
                     /locus_tag="PK154_08790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW11193.1"
                     /translation="MAGSLPPLNRLVPPYIQKIEAYIPSKPDDVLKKMYNCPFLYRMN
                     NNENVIGPPEKAIEAIMDFDPARAAIYPSGDGYHLRHRLSEVFGLPSCSFIIGNGANE
                     LIAFVIKAFCWKGDNIITSNKTFAVYEWTAEFSGFESRLVPLKDNTFDDEGILKRIDD
                     RTRVIFICNPNNPTGTYWNEARLRRFLERVDGEQIVVVDEAYAEFVEQPDFPESMRLI
                     REFPNLVVFRTFSKIYGLAALRIGYLAGDRSVVDVIRRTCVVYSVNTLAQEAALAALG
                     DDGTHIARTRSLIRASKEYLLPELDRMDLPYISGEGNFVMIRLPFSDTLAYRRLMRKG
                     YMIRTMTGFRFPNHIRVTLHDVPVMEGFVDALRNTLA"
BASE COUNT          867 a         1064 c         1155 g          895 t
ORIGIN      
        1 cctcataaat agtgtcgtgg gatatggagc agagcacact gaacctgaat gattcccggc
       61 gagcgcggac gctcttcatc ctgtacctga ccgggttcac cacgatgctg ggtatcggca
      121 tcatcacacc ggtcctcccg atctatgccc agatgatcgg tgccacgggg ttctggatcg
      181 gggcgatatt ctcggtattt gccctgtccc gtaccatctt catgccgttt gtcggcaggc
      241 tgtcggatgc gggagggaga aggaggttta tcctgctcgg ccttgccggg tatgccttct
      301 tctccgcact gtatgtcccg gcggataatg tcctcatcct gaccattgtc cggttttttc
      361 acggaatggc agcagcgatg gtctatccca tcgccaatgc ctatgtcgcg gatatggcgc
      421 gggtgggtga agagggaaag acgatgggag ggttccagag cgctgcattc ctcgggatga
      481 gcttcggtcc gcttctctca ggcataatgg tggatttgtt gtatgtccag agtgctttta
      541 tcgcacttgc gggactgtcg ctgacagctc tgatcatttg catagcgtac ctcccggagt
      601 atcatgtccc gatgagagag atcccgccgc tcaggtcggt ctttttcagc agaggaatgc
      661 gagttcccat catctttttc tttacctaca cggcgggata cacggcgttt atggtcttct
      721 ttccggttct cgctcacgca gaccagatga ccgtcagcgg gatcggagca ctcatcttca
      781 tctcctcgat ctcaacagcg gtcttccagc ggctttccgg gagatatgcc gatctgttcg
      841 acaggcacct ggtcctctcc accggggccg gggtcatggc ggtgtctctg ctgcttttca
      901 cgatgagcga gggctttctt tccttcctcc tcacctcggt gctcctgggt gccggcatcg
      961 gtatatcgct tacgaccgta tcggcaatgg cggtcattga aggaaggaat cttggccagg
     1021 gaagtgttga aggggtcatc aacacggcgc aggggattgg tatcgttatc agcccgctcg
     1081 tcctgggact ggtcatggac tttacaggaa tatcgatggt atttctcaca gccgcaggag
     1141 tatcggggct gatggcactc gcaatgatgc ggtttgtata tcgggaaacg cccgctcagg
     1201 cccggaatgc agaatagaaa gattgatata gcactgtata attgatattt ctattggaac
     1261 cgcgggacca aaacaggcat gccggttccg tgaatcacga agatcatcgg gtgtttattc
     1321 tcacaataaa gctcaggaat ggttagccat catgaacata cctgaaacag cagaactcat
     1381 cgtcccgatt gcgatccgga atgtccctat cgtcaccggc atggtcgaac aatcggcaac
     1441 tatttttggc ctggacaaag agggcgccca gaacctcggg cttgcagcgg aagaggtgtt
     1501 caatgtcctg gcatctgccg caaggcctga tgaaaagatc cggatcgtcg tcagacaggg
     1561 cggctactat gtggaagttg catgttattt ctcgcgccat gcaatcccga tcaccgcgct
     1621 gaacatcacc aaaatacccg atcctgacga tgaatcggac atcgaaaacc tcggcatcat
     1681 ccttgccgcc aaggtggtcg accggctcca gctggtcagg agccgtcagg gagagatggc
     1741 tctccgtttc actatcgagc gacggtatcc ggagcaggtc atcccttccg ggatcccgcc
     1801 tgcggggcat tattcggtcg gggagggagg gttcgaagag atcaagcagt ttgcttcacg
     1861 tgtctcgggg atgtatggtg ccaattcccc cggattcttc cggttcccgg gaaagatcgt
     1921 ggatatggtg aagagccggg aattcggtgt tctcctggcc cttgacggaa aaggcaacgt
     1981 cgggggcggg atgctgtgga aatggggggg gaagatgatc gactcctttg ggccctacat
     2041 ctttacacca caacccgggc ttgtcggtga gatcgtgcag ggagtactgg aaaaagtggg
     2101 caggaagggt gcagtctgca tcgtgaacag gatgccggcc gacgatctcc tccgcaattt
     2161 ctttgaggaa cttgtcgaaa gctgccctgc aaacaggatc cggaccacgg catgccaccc
     2221 ccccctgtat cgccagctgg aggaggatac ggggtccgtt gcatatgtcc atcccgaagt
     2281 agagattttt gtaaaagaat gctatgaacg gctcgtgctg ccccggagcg tggtcgccac
     2341 gacccatgca ggagagttca tcgagcctca cgcggcattc ggcgtccagg ttgataaaaa
     2401 acagaacctt gcggtcattt caacgatagt cgcaggaatc gatgcggagg aagtccttgc
     2461 agggtacgtc gcctctctgt acgacgaggg gataacgcgg ctcgagttcg agatcgatct
     2521 cggggacgag acgactccgg tgctggttcc tgctctccat gccgtgggat tcattccctg
     2581 ctatgtcctg ccctgggggg gtagagggga tattcttgtc ttcgaatgcc agggaccgcg
     2641 aggcttctga tatggccggc agccttcccc cgctcaaccg gctcgtcccg ccatatatcc
     2701 agaagatcga ggcatatatc ccgagcaagc cggacgatgt cttaaaaaaa atgtacaact
     2761 gcccgttcct gtaccggatg aacaacaacg agaacgtgat cggccctcca gagaaagcca
     2821 tcgaggcgat catggatttc gatccggcga gagcggcaat ctacccaagc ggcgacggct
     2881 accacctgcg ccaccggctc agtgaagttt tcggcctccc gagctgttcg ttcatcatcg
     2941 gtaacggtgc aaacgaactg attgcatttg tcattaaggc attctgctgg aagggagata
     3001 atatcatcac gtccaataag acgtttgccg tttacgagtg gacagcggag ttttccgggt
     3061 tcgaaagccg gcttgtaccg ctcaaagaca acaccttcga tgacgaagga atcctgaaac
     3121 ggattgatga caggaccagg gtgatcttca tctgcaaccc caacaacccc acgggcacgt
     3181 actggaatga ggccaggttg agaaggttcc tggaacgcgt cgacggggag cagatcgttg
     3241 ttgttgacga ggcttatgcc gagtttgtcg aacaaccgga ttttccggag agtatgcgtc
     3301 tcatccggga gttccccaac ctcgtcgtct tccggacgtt ctcaaagata tacggacttg
     3361 cagccctgcg catcggttat cttgccgggg acagaagcgt ggtcgacgtc atccgccgca
     3421 cctgcgtggt ctattccgtg aacacgcttg cacaggaggc cgcgctcgcg gcgctggggg
     3481 acgacggcac ccatatcgca cggacacgga gcctgatccg ggcatcgaag gaatatctcc
     3541 tgcccgaact ggaccggatg gacctcccgt acatcagcgg ggaaggcaat ttcgtcatga
     3601 tccgcctgcc gttctccgat acgctcgctt accggaggct catgcggaaa ggatacatga
     3661 tccggaccat gaccgggttc cgattcccca accatatccg ggtcaccctg cacgacgtgc
     3721 cggtgatgga agggttcgtg gatgcactca ggaacacgct tgcctgacac aacgtgcgga
     3781 tgcagtggac ccatttcggt atagcatgag atttaatgat gaacaaggga tgaaaaaatg
     3841 acgaccgaac cagaatacac gatcgatgat ttcaggccgg aggatgcaga gggcgtcgtt
     3901 gcactctacc gcgccgtgta cggcgaaaat tacccgattc gcagtgtata cgatgcaagg
     3961 gagatcatcc ggcaggaaga g
//
LOCUS       DAOOCO010000169         3976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21551_length_3976_cov_4.745218, whole genome shotgun sequence.
ACCESSION   DAOOCO010000169 DAOOCO010000000
VERSION     DAOOCO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21551_length_3976_cov_4.745218"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK154_08795"
     CDS             <1..183
                     /locus_tag="PK154_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11194.1"
                     /translation="VQGVLPYPGHHPPVDHEGLVNSGIGCGESYQESTLPRDRESDPQ
                     RSFTWPSTSCSSFSPF"
     gene            385..1251
                     /locus_tag="PK154_08800"
     CDS             385..1251
                     /locus_tag="PK154_08800"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPW11195.1"
                     /translation="MKWRTDKTSTHVEDRRGTSSRIPGGAAGGIGGIGLIIILVAAVF
                     LGVDPGQFIQGNDVSTESYGQQPEYTQADQEVFNSSARVFTDTEQTWSIIFEDQLGRT
                     YSPPNLVVFEDYVSTGCGQATSQTGPFYCSADQRVYIDLDFFKELERQFKAPGDFAQA
                     YVISHEVGHHVQNLLGTLQYVNRQQARLNEADANALSVALELQADCYAGVWAYQAQYG
                     DLILERGDLEEALNAASAIGDDRIQMQSMGYVVPDTFTHGTSAQRVEAFRKGMDAGMV
                     TDCNCAELPTVG"
     gene            complement(1697..2524)
                     /locus_tag="PK154_08805"
     CDS             complement(1697..2524)
                     /locus_tag="PK154_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11196.1"
                     /translation="MGLDTQEDCLITSDPGPGKRRTLVIAGLIILIIAAAGIGWVIFQ
                     FDRIAGESERHDYAYSLTLSLSSGIENVTLLLPVPELNGTTSLGDMIANGTGYGVPED
                     WQISIAIVNGTPFLQVRAARMVPGYHGFPIPVEPGAQPSGTPPPTATGYSEETPVLLP
                     VEIGATMETEVPVDTRDPIGREPLFSQGGEYAPRLKTTPPCEGMEYTHPVRLYLSYTA
                     DNPVPVSLVARVSGTNSIWRGGWTFNSYRDQVLVEAGDRTGWIETDAVLVVGEGTYW"
     gene            complement(2532..3305)
                     /locus_tag="PK154_08810"
     CDS             complement(2532..3305)
                     /locus_tag="PK154_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPW11197.1"
                     /translation="MDIGMRDRFTRLWNQYFEGADLPIAFWIGPAGEGVPRLPAPKRW
                     RCMVCDLARVRKGHPVVIDHKSLSCGGARLYLGYATERPPFFRYFLSCGKPGVVEGER
                     YKRTPEIVDEFDQNLVHIPSEGRCYTFKCWDQLTREDIPDVVIFFARPEVISGLFTLA
                     GFDQGDPEGGVICPFGSGCSSIVYYPWLEQQKEHPRAVLGMFDPSARPCVMPDVLSMA
                     IPMRKFARMIDYMEESFLVTPTWERVREKIRRSAAFNGL"
     gene            3365..>3976
                     /locus_tag="PK154_08815"
     CDS             3365..>3976
                     /locus_tag="PK154_08815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW11198.1"
                     /translation="MVDRCYLAHDRTIPSMHRIIDWNELWKAIHVASPERTKKAGDPA
                     ATWDKRAAAYHRISHEETEATKRDLGFIDLRPGDTVLDMGAGTGRLAVPLAGRAAHVT
                     ALDPSGGMLGILRKRMEEAGHHNYSCVQMKWEEVVLGRDIGPHDIVIAAFSLGFYDLA
                     AALAKLDQAAKRSVYLFWHAGEWRGPEEMGLYRAVFGDEGAMQR"
BASE COUNT          877 a         1166 c         1149 g          784 t
ORIGIN      
        1 gtgcagggag ttctgcccta cccaggccat caccccccgg tggatcatga gggtctcgta
       61 aactccggga ttggctgtgg ggagtcatac caggagagca cccttccaag agatcgcgag
      121 agcgaccccc aacggtcttt tacctggccc tccacttctt gttccagttt ctcccccttc
      181 tgattcaagg ccccctcccg ggattaggag ggttgcgtga cagccggcgg aattcggact
      241 gattcctcac aggaaatccc tggaatcccg tgctctcctt tcatcaatca gaactttttt
      301 cgtcacatcc tcacaatgag tcctttagcg aacaaaggtg aacgcaatga ccaatgcaac
      361 agacctggag agggggaaca ggcgatgaag tggcgaaccg acaagaccag tacccatgtt
      421 gaggaccgga gaggtacctc cagtcgcatc ccggggggag cagcgggagg gatcggcggg
      481 attggactga tcatcatcct cgtcgctgca gtcttcctcg gggtcgatcc gggccagttc
      541 atccagggga acgacgtctc aaccgaatcg tacgggcagc aacccgaata tacccaggcc
      601 gatcaggaag tcttcaactc atcggcacgg gtattcaccg acacggaaca gacctggtcc
      661 atcatctttg aagaccagtt agggagaacg tatagcccgc caaacctggt ggtgttcgag
      721 gactacgtga gcaccgggtg cggccaggcg acctctcaga ccgggccgtt ctactgctct
      781 gccgaccagc gtgtgtacat tgatctcgac tttttcaagg aactcgagcg gcagttcaag
      841 gctcccggtg actttgccca ggcctatgtc atctcccacg aggtaggcca ccatgtgcag
      901 aacctcctcg gtaccctgca gtatgtgaac cggcagcagg cccggttgaa cgaggccgat
      961 gccaatgcac tctcggtagc acttgaactc caggccgatt gctatgccgg ggtctgggca
     1021 taccaggcgc agtatggaga cctgatcctt gaacggggtg acctggaaga agccctgaac
     1081 gctgcaagcg ctatcggtga tgaccgaatc cagatgcagt ccatgggata cgtcgtgccc
     1141 gatacgttca ctcatgggac ctcagcccag agggttgaag cgttcaggaa ggggatggat
     1201 gcaggaatgg tcactgactg caactgtgcc gaactcccga cagtcggata agatctggtc
     1261 atctcttttt ccattgaaaa tcacttcata attttcataa tttatgcccg tatcccgaaa
     1321 tcggggctca tgggtggttt atatgaaaat gcctccggca ttttcatctt ttatgcttgt
     1381 ggcccgcaat ggaatcatgt tattttttta tttgaggtaa gaaggctttc agaccaaaac
     1441 gcccttgtcc atcacctgca tactgcaggc gtgtctcctt ccagatactc atgaagggat
     1501 atatccccag gtataccggg acaacaagga tcgggacgat gacaaaccag aggcaaagat
     1561 agttactgac agggtcagga ggatgatttc gatgatagcg gagctgacaa ggtacattgg
     1621 atcacaagtg acaggtgagg agtaatggga gttcaaacct gaaaaaccgc ggggagatgt
     1681 tgccccgaca gggctctcac cagtaggtcc cttctccgac aacgagcacc gcatcagtct
     1741 cgatccaccc ggtccggtcc ccggcctcca ccagcacctg gtcccggtaa gagttgaagg
     1801 tccagccgcc ccgccagatc gagttggtgc ccgagacccg ggcaacgaga gagaccggga
     1861 cagggttatc ggccgtgtag gagagataca ggcggaccgg atgggtatac tccattcctt
     1921 cgcaaggcgg cgtggttttc agacgtgggg catactcccc gccctggctg aagagcggct
     1981 cgcggccgat cggatcacgg gtatcgaccg gaacctctgt ttccatggtc gctccaatct
     2041 caaccgggag cagaacaggg gtctcttccg agtatccggt agctgtggga ggcggggtcc
     2101 cgctgggctg tgcaccgggc tcaaccggga tcgggaagcc atggtaccca ggaaccatcc
     2161 gggctgcccg gacctggagg aaaggcgtac cgttcacgat cgcgatggag atctgccaat
     2221 cttccgggac cccgtacccg gtcccgtttg caatcatatc accgagggaa gtggtgccat
     2281 tcagctccgg caccgggagg aggagggtca cattctcaat cccggacgag agggagagcg
     2341 tgagagagta agcataatca tgccgttcgc tctcccccgc tatccggtcg aactggaaga
     2401 tgacccagcc aatacccgca gcggcgatga tgagaatgat gagaccggca atcaccaatg
     2461 tccgccgttt tcccggcccc ggatctgatg tgatcaagca atcctcctgt gtatccagcc
     2521 ccatctcctc ctcacaatcc gttgaatgct gcactcctcc gaatcttctc tcttacccgc
     2581 tcccaggtgg gggtgacaag aaaactctcc tccatgtaat cgatcatccg ggcgaatttc
     2641 ctcatgggga tggccatcga gaggacatcc ggcatcacgc agggtcgtgc cgatggatcg
     2701 aacatgccaa ggacagccct cgggtgttcc ttctgctgct caagccaggg atagtagacg
     2761 atcgatgagc accctgatcc gaagggacag atcacaccgc cctccggatc tccctggtcg
     2821 aaaccggcca gggtgaataa cccggagatg acctccggcc gggcaaagaa gatgaccacg
     2881 tccgggatat cctcccgggt cagctggtcc cagcacttga aggtgtagca cctcccttca
     2941 gaggggatat gaaccaggtt ttggtcaaat tcatccacga tctccggggt ccgcttgtac
     3001 cgctcccctt cgaccacccc cggttttcca caggagagaa aataccggaa gaacggggga
     3061 cgctcggtgg catacccaag atacagtctc gcgccgccgc aggacaggga cttgtggtcg
     3121 ataaccaccg ggtgcccttt ccgtacccgg gcaaggtcgc agaccatgca cctccaccgt
     3181 tttggcgctg gcagcctcgg tacaccctcc ccggccggcc cgatccagaa cgctatcggc
     3241 aggtccgccc cttcaaaata ctggttccac agccgggtga accggtcccg catgccgata
     3301 tccatggagt cctatcaggg agcggctgga taaaacggtt gcgatcgccc gggcggatct
     3361 gggcatggtt gaccgatgct atttagccca tgacagaaca ataccctcca tgcaccggat
     3421 tatcgactgg aacgaactct ggaaggcaat ccacgtggcc tcccctgaac ggaccaaaaa
     3481 ggccggcgac cctgctgcca cctgggacaa gcgtgctgcc gcctaccatc ggatcagcca
     3541 cgaggagacg gaggcaacaa agcgggatct cgggtttatc gatctccgcc cgggcgatac
     3601 ggtccttgac atgggggccg gaacgggaag gctggccgtc ccccttgccg ggcgtgcagc
     3661 acacgtgact gccctggacc cttcaggagg catgcttggc atcctccgga agcggatgga
     3721 ggaggcaggg catcacaact actcgtgtgt ccagatgaaa tgggaggagg tcgtcctcgg
     3781 ccgggatatc ggaccccacg acatagtcat cgccgcattc tcgctcgggt tctatgatct
     3841 cgcagcagcc ctcgccaagc tcgaccaggc agcgaaacgg tcggtctatc tcttctggca
     3901 tgctggcgag tggagggggc ccgaggagat ggggctctac cgtgcagtgt tcggtgatga
     3961 gggggcaatg cagcgg
//
LOCUS       DAOOCO010000170         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21608_length_3968_cov_3.668030, whole genome shotgun sequence.
ACCESSION   DAOOCO010000170 DAOOCO010000000
VERSION     DAOOCO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21608_length_3968_cov_3.668030"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK154_08820"
     CDS             complement(<1..984)
                     /locus_tag="PK154_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11199.1"
                     /translation="MQFRLMKGHTGMCDSNQHFFKYSAMIALLIISMIGIVSGGTTVT
                     PLLSSDIVIDSDGVFLYGDVAFDGTHYLSVWSDKTVSTTGSTYSVSGRFIDVNGVPYG
                     SSFTINPSYANDIAVAFNGQTYLVTYIGRNEYYDNTVYGNYVDVNGNVGPKILIATDS
                     NSISNVDAISDGTGFLLVWDQYIGSPATNEHIYGQFLTSSEKSGSPFIISNTDVRRRW
                     NPTITYGGGNYLVVWMQAPWDNSQLDIYGARVSPSGMVLDPLNFGVSTAPNGQGWGWM
                     KGGASYDGTNYLVGWQDYRNSKGYAQIFGTRISPSTGALLDGPSDTGGKLIA"
     gene            1282..1473
                     /locus_tag="PK154_08825"
     CDS             1282..1473
                     /locus_tag="PK154_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11200.1"
                     /translation="MVHLVGGDCERLGSFFEQEACYRLILLCVLPHLAGPVREECDEL
                     ITRFHGVKAGPACMGHLPL"
     gene            complement(1803..2162)
                     /locus_tag="PK154_08830"
     CDS             complement(1803..2162)
                     /locus_tag="PK154_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11201.1"
                     /translation="MSTWTVTEHLESPFEKVGRLYVQGENLVIRSDLDCRGFSIPLSD
                     LPAVLDGDPERVFLLGSETEVGTIRLSSSGRAMNFTIEPFYYTTPLQSITKMLAGEQR
                     KAPLFVGREQVGGVNEE"
     gene            complement(2209..>3968)
                     /locus_tag="PK154_08835"
     CDS             complement(2209..>3968)
                     /locus_tag="PK154_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11202.1"
                     /translation="KGKSHAFSTMLRQVPPRFRLEGAMSNKALFYLENLQPGSVIVMD
                     DRALSEEMAEILKGATTAFGIPFLYRTVSKDRKALVCTLPERCVWWVAKVEGAGDDQV
                     FNRMLTCWIDDSEEQDTRVLARILAREETVPHSTGEERPEILTCQTMWDLIGSHQYHV
                     VIPFASRIQFKEAGNRRNAEMLLDLVKANTVLCSHQREQDHAGSRIHLTATREDFAEA
                     ARLYQLLNGTAGGQMTKLTKRESDLIAIIRNLGRNEFTIPQLQKATGWSSGNLHKYLH
                     GHVTRGRAYAGLMEKCPAIAWCDRTVVTEEEECVSMRRRTNAYTFDPDLYQSWSGGGG
                     VWLDEDPGDPSTDFNQPSTHDEGIENDGNGSDSENNDYNKNIYVRTSDYFNKNRDTEQ
                     PKEAGVQACSSGGDPAGIEGSPSNTVHRTGNEKKTAYNNPILLQPPLQRRPDAEGEEN
                     TDRSLPGLIPRIDPRDYQPLEVPDHTYCRLCGSPWTHYVLKLSSERKRREKDEGKPNQ
                     ICRHCYARAKKRSQQSATILPGTCDPARMERLNSPFSRCSICELDPAVYLDRSTGTKL
                     CETCYQRISRSAEHGEVSG"
BASE COUNT          853 a         1051 c         1036 g         1028 t
ORIGIN      
        1 cgcaatcagt tttccccccg tatcggatgg accgtcaagt aaggctcctg ttgaagggga
       61 gatccgcgta ccaaatattt gggcgtaacc cttgctattt cgataatcct gccacccaac
      121 aagataattt gtaccatcgt acgatgcgcc acctttcatc cagccccagc cctggccgtt
      181 gggtgcggta gaaactccga agttgagcgg atcaaggacc atccctgaag gactgactct
      241 tgcaccgtat atatccaatt gactgttatc ccagggcgct tgcatccaca cgacgaggta
      301 attccctccc ccgtaagtaa tcgtcggatt ccaccgtctc ctgacatcag tgttgctgat
      361 gatgaaaggt gatccggatt tttctgatga tgtgaggaat tgcccgtaga tatgttcatt
      421 tgttgctgga gatccgatat attgatccca taccaggaga aatcccgttc cgtcggaaat
      481 tgcatcaaca ttgctgatgc tgttcgaatc ggttgcaatt aggatctttg gaccaacatt
      541 cccatttaca tcaacataat tcccataaac ggtgttatcg taatattcgt tcctgccgat
      601 ataggtaaca agatacgtct gtccattaaa agcaacggca atatcatttg cataactggg
      661 atttattgtg aaggaactcc catatggcac accattaacg tcaataaatc gaccgcttac
      721 ggaatacgta ctgccagttg tactgaccgt cttatcactc catactgaga gataatgggt
      781 accgtcaaaa gcaacatcgc cgtataaaaa tactccatca gaatcaataa cgatatcaga
      841 ggaaaggagg ggggtaaccg tagtcccacc actcactatt ccaatcattg aaataatcag
      901 cagagcgatc attgcagaat atttgaaaaa atgttgattt gaatcacaca tacctgtatg
      961 tcctttcatt aaacgaaact gcattgtttt tactccttat gcttcagtaa tgccagatat
     1021 taattggaat gggtatggaa aggtttgaga attacagcac ttgctatgct atcgtaccca
     1081 acctacacat atgctgatta atcagtcctg acctggccta tccttattgg caaggatatt
     1141 tctccggact ccccaaccat ctggtgtttt ttattgttga aatgataaat cttatgaaat
     1201 tgtttttaaa aactacgata attttactgt gattaccaaa aagccggttt atggcgggta
     1261 cacggtggcc ccgaagatag agtggtccac ctcgtagggg gtgactgcga acggctgggc
     1321 agcttttttg agcaggaagc atgctaccgg ctgatccttc tttgcgttct tccccaccta
     1381 gccgggccgg tgcgagagga atgcgatgag ctcatcaccc ggttccatgg tgtaaaagct
     1441 ggcccggcat gtatggggca tcttccgctc tgattccagc aggagccccg atttgtcgac
     1501 agacaacttc ggggatcatc ccgggtcggg gggaatagta tacttccttc gagttagatg
     1561 tgggtttttt cccgaggaaa atggggaatt ttcaccgctt gatagggtga cggcatcgac
     1621 atatgcatgg aggtatactt tttattcgat cttcatcacc taccctttta taaaaaccaa
     1681 acagctatcc gtcatggaat acttgaactg tccgttctgt agggttgatg cagagaagct
     1741 ggtcatcgat caggaatagg tcattttacg gagctgaacc atgagtagat gaagggattc
     1801 catcattctt cgttcacccc ccccacctgc tcccttccga caaacagcgg ggctttccgc
     1861 tgctccccgg caagcatttt tgttattgat tgcaatggcg tcgtgtaata gaacggctcg
     1921 atggtgaagt tcatcgccct ccccgagctg gagagccgga tggtcccgac ctctgtttcg
     1981 ctgcccagca aaaagacccg ttctggatcc ccatcgagta ctgccggaag atccgaaagg
     2041 gggattgaaa atccccggca atccaggtcg gaccggatca ccagattctc cccctggaca
     2101 tacagcctgc cgaccttctc gaacggggac tcgagatgtt cggtcacggt ccaggtgctc
     2161 atgggctcct cctccctgcc tggtcatgcc gggaccggtg caccgaggtc atccgctcac
     2221 ctcaccgtgt tctgctgacc tgctgatccg ctggtagcag gtctcgcaga gttttgtccc
     2281 ggtgctccgg tcgaggtaga cggcgggatc gagctcgcag atggagcacc tgctgaaggg
     2341 cgagttgagc cgttccatcc tggcgggatc gcaggtcccg gggaggatgg tggcggactg
     2401 ctgggatctc ttctttgccc tggcataaca gtgcctgcag atctggttcg gtttcccctc
     2461 gtctttctcc cgccgttttc gttcgctgga aagcttcagg acatagtgag tccatgggct
     2521 gccacacaac cggcagtagg tgtggtccgg gacctccagg ggctggtagt cccggggatc
     2581 gatccggggt atgagacctg gcagggatcg atcggtgttc tcctcccctt cagcatccgg
     2641 ccttctctga agtggtggtt gaaggagaat cgggttgtta tatgccgtct ttttctcatt
     2701 tccggtgcga tgtaccgtat tcgatgggct cccttcaatc ccggcagggt cacccccaga
     2761 cgaacaggcc tgcacaccgg cttctttggg ctgctctgta tccctgtttt tattgaagta
     2821 atcagaagta cgtacatata tattcttatt ataatcatta ttttctgaat cggagccatt
     2881 tccatcattt tcaattcctt catcatgggt tgaaggctgg ttgaagtcgg ttgaaggatc
     2941 gccaggatcc tcatccagcc agaccccacc cccgccactc catgactggt agaggtccgg
     3001 gtcgaaggtg taggcattgg tcctcctcct catggaaacg cactcctctt cctcggtaac
     3061 caccgtacgg tcacaccagg caatcgccgg acatttctcc atcagcccgg cgtacgccct
     3121 gccccgggtg acatgcccgt gaaggtactt gtggagattg ccgctgctcc acccggtggc
     3181 cttctggagc tgcgggatcg tgaattcgtt tcggccgagg ttccggatga ttgcgatcag
     3241 gtccgattcc cgtttggtga gcttggtcat ctggcccccg gcagttccgt tcaggagctg
     3301 gtacagccgg gctgcttcgg caaaatcttc cctggttgcg gtaagatgga tgcgtgatcc
     3361 cgcatgatcc tgctctcgtt gatgggagca tagcacggtg tttgccttga caaggtcgag
     3421 gagcatttca gcgttccggc ggttgcctgc ttctttgaac tgtatcctgc tcgcaaaggg
     3481 gatcacgaca tggtactggt gggatccgat caggtcccac atcgtctggc aggtgaggat
     3541 ctcaggtcgt tcctctcctg tgctgtgggg aacggtctct tcccgggcga ggatgcgggc
     3601 gagcacccgg gtgtcctgct cttcggaatc atcgatccag caggtgagca tccggttgaa
     3661 gacctggtca tccccggctc cttccacttt tgcgacccac cagacgcacc gttccgggag
     3721 ggtgcagacc agggctttcc ggtccttgct gacggtgcgg tacaggaagg ggatcccaaa
     3781 ggcggtggtc gcccctttca ggatctcggc catctcctcg gaaagggccc tgtcgtccat
     3841 gacaattacg gaccctggct ggagattctc gaggtagaag agggccttgt tgctcatggc
     3901 gccttccagc cggaagcggg gtgggacctg ccggagcatg gtcgagaagg catggctctt
     3961 tcctttcc
//
LOCUS       DAOOCO010000171         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21674_length_3959_cov_3.990010, whole genome shotgun sequence.
ACCESSION   DAOOCO010000171 DAOOCO010000000
VERSION     DAOOCO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21674_length_3959_cov_3.990010"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK154_08840"
     CDS             complement(<1..319)
                     /locus_tag="PK154_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPW11203.1"
                     /translation="MADPVASSDLFAWVILPLLIFFFRICDVSLGTIRVIFIAKGLKY
                     IAPIIGFFEVIIWLLAIGQVMNNVSNVACYIAYGGGFAAGTLLGVTVEERLSIGTVVV
                     RVIS"
     gene            complement(347..1594)
                     /locus_tag="PK154_08845"
     CDS             complement(347..1594)
                     /locus_tag="PK154_08845"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPW11204.1"
                     /translation="MAEQNLFEEVKRHLCSCSADLQITPNLEAVMKMPMRELHVSIPV
                     RMDDGSIRAFSGFRVQYNDALGPTKGGIRFHPDETIDTIRGLAAIMTWKCALHNLPLG
                     GAKGGVICNPKTMSSGEIERLSRAYIQATSHFIGPDRDIPAPDVYTDSEVMAWMMDEY
                     SRIFRKTTFGVITGKPVSLGGSEGREDATARGGWFVIREAAKDFGIVLEGAKVAIQGF
                     GNVGAHAAIIGQEQYSVRVVAVSDSRGGVFAREGLDVPALLEHKKKTGSVLDFPDARN
                     ITNEELLALDVDILIPAALENVITIDNASRVKARFIAEFANGPVSGDADALLHARGVP
                     ILPDILCNGGGVIVSYLEMVQNFNLDHWERGEVIARLERKMVDAYRAVYNLASGNNVP
                     MRQAGYTIAVGRVVKAMKYRGWV"
     gene            complement(1645..2496)
                     /locus_tag="PK154_08850"
     CDS             complement(1645..2496)
                     /locus_tag="PK154_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW11205.1"
                     /translation="MPEKNLITGAQNTWCPGCGNFTLQFALRNAIKGLIADGVPLENI
                     VLVTGIGCHAKMGDYLDINSFYSIHGRTIPVATGIKLANPDLTVLCCAGDGDAYAEGL
                     DHLIFGAKRNTDITVIVHNNRVYGLTTGQYTPTSPLGFRGKSTPQGAPEEPFNPLILM
                     LASGAGFVARSTSRKMPHLSGIIGQALRHKGFSYVDVLQICATYFNVSELYDRQGYEL
                     SDHDPSDFSAACAKAEEWDYNSDAPIAFGLFYQEEKTSFEERFSRHPASPGSRNEQIR
                     AFLEMKR"
     gene            complement(2496..>3959)
                     /locus_tag="PK154_08855"
     CDS             complement(2496..>3959)
                     /locus_tag="PK154_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW11206.1"
                     /translation="HRDRCDYLLALSQDTLDLHRDLIHDDTVVLYDSGRVHGKGLGIP
                     VEEILKAEGASAVMGNTCIIGAFAKAAGIPFEVVETVLRRQVPKDPEKNLVIARKGYD
                     AVREQKVIPRAGHTCCPLLTGNEAIGLGLLHGGLDAFVAYPMTPTSNLLHFLAGVAGQ
                     FALTVVHPENEIAVMLMALGFSYAGKKTAVGTSGGGFCLMTEGFSFSGSSEVPVVVVM
                     GQRTGPSTGLPTYTGQTDLNFVLHAGQGEFPRLIVAPGDAEQAYVWSSIALSLAWKCQ
                     VPAVILCDKTLCEGTYSLDEKAIGPPETSDLQQSGISGQYRRYSITDNGISPLQFPPL
                     KDAAIKVNSYAHDEDGITTEKADMVRLMTEKRQRKYEALKGEIDALECVKSYGTSSAR
                     TALLCWGSTLLVCKEAAKRHGFGVIQPVVLSPFPEEQLKSVIEGTPNIISVEENTEGQ
                     LRLLCHQHGIPVASHIAKIDGRPFSVEELDARLGEVP"
BASE COUNT          832 a         1151 c         1081 g          895 t
ORIGIN      
        1 ttgagatcac ccggacgacc accgtcccaa tagaaagacg ttcttcgaca gtcaccccaa
       61 gaagggtccc tgccgcaaaa ccccccccgt aggctatgta gcatgcgaca ttcgagacat
      121 tgttcatcac ctgccctatg gcaaggagcc agatgataac ctcaaaaaaa ccgatgatcg
      181 gagcgatgta cttcagaccc tttgcgatga agatgaccct gatcgttccg aggctcacat
      241 cacagatgcg gaagaagaat atcaggaggg gtaatatcac ccatgcaaag agatcggatg
      301 atgctacagg atctgccatt gccttcccac catattacag gatatttcag acccaaccgc
      361 gatatttcat tgcttttacc actctcccga ccgctatggt atacccggcc tggcgcatcg
      421 ggacattgtt cccggaagca agattataaa ctgcacggta ggcatcaacc atcttcctct
      481 cgagcctggc aatgacctct cccctttccc agtggtcaag gttgaagttc tgcaccatct
      541 cgagatatga gacgattacc cctcccccat tgcaaaggat gtcgggaagg atcggaaccc
      601 ccctggcatg gagcagggca tcagcatcac cgctgacagg accatttgcg aattctgcaa
      661 taaaccttgc cttcacccgg ctggcattgt cgatggtgat gacattctcc aacgcagcgg
      721 gtatgaggat gtcaacatca agtgccagta actcctcgtt cgtgatattc ctggcatcag
      781 gaaagtccaa gacgctcccc gtcttcttct tatgttccag gagggctggg acgtcaagac
      841 cctcccgggc aaacaccccc ccgcggctgt cgctcacggc tacgaccctg acggaatact
      901 gctcctggcc gatgatggca gcatgtgcac caacgttccc gaatccctgg atcgcgacct
      961 ttgccccttc gagaacgatg ccgaaatcct ttgcagcctc cctgataaca aaccatcccc
     1021 cccgggcagt ggcatcttcc ctgccctcgg atccccccag gctgaccggc tttcccgtta
     1081 tgaccccgaa cgttgtttta cggaagatcc tggagtactc gtccatcatc cacgccatga
     1141 cctcagaatc ggtatagaca tccggtgccg gaatatcccg atccgggccg ataaagtggg
     1201 aggtcgcctg gatataggca cgcgagagcc gctcgatctc cccggaagac atcgtcttcg
     1261 ggttgcagat caccccccct ttcgcaccac cgagcggcag gttatgcagt gcacatttcc
     1321 aggtcatgat ggcggcaagt ccccggatgg tatcgatcgt ctcatccggg tggaaccgga
     1381 ttccgccctt tgttggtccc agggcatcgt tatactggac acggaatccg gaaaatgccc
     1441 tgattgaacc gtcatccatc cgtaccggga tggagacatg gagctcacgc atgggcatct
     1501 tcatgactgc ttcgaggttg ggggtgatct gaagatctgc actgcaactg cagagatgcc
     1561 gcttgacttc ctcgaataaa ttctgttccg ccacagtatc ttctccttcc tgtttccggt
     1621 tttttctctc ttttcacaga gctgttatcg tttcatttca agaaatgccc gtatctgttc
     1681 attccggctt ccaggagaag caggatgcct gctgaaacgt tcctcaaaac ttgttttttc
     1741 ctcctggtaa aaaagcccga acgcaatggg tgcatcgctg ttatagtccc attcttcagc
     1801 tttggcgcat gcagcggaga aatcggacgg gtcatgatct gataactcgt atccctgcct
     1861 gtcgtagagt tctgatacat tgaaataagt tgcgcagatc tggagcacat ccacgtagga
     1921 aaatcccttg tggcggagtg cctggccgat gatccccgac aggtggggca tcttccttga
     1981 ggtactccgg gcgacgaatc cggccccgct tgcaagcatc aggatgagcg ggttgaaggg
     2041 ctcttcagga gcaccctgag gggttgactt tccccggaag ccaagcgggg aggtaggggt
     2101 atactggccg gtggtgaggc catacacgcg attgttgtgg acaatcacgg tgatatcggt
     2161 gttccttttc gccccgaaga tgaggtgatc cagcccctcg gcataggcgt caccgtcacc
     2221 tgcacaacag aggaccgtaa ggtcggggtt ggcaagcttg atcccggtcg cgaccgggat
     2281 cgtccggccg tgaatcgagt agaagctgtt gatgtcaaga tagtcgccca tctttgcgtg
     2341 gcacccaatc ccggtgacga gcacgatatt ctcaaggggg acgccgtctg caatgagccc
     2401 tttgatcgca ttcctgagtg caaactggag ggtaaagttc ccgcagccgg ggcaccaggt
     2461 gttctgggca cccgtgatca ggttcttttc aggcatcatg gcacctcccc cagcctggca
     2521 tccagttcct cgacagaaaa cggacggccg tctatcttgg caatgtgtga ggcgacaggt
     2581 attccatgct ggtgacagag gagcctgagt tgcccttcag tgttttcttc aacggagatg
     2641 atgtttggtg taccttcaat aaccgatttg agttgttctt cagggaatgg tgagaggacg
     2701 acgggctgga tcactccgaa tccatgccgc tttgcagcct ctttgcagac caggagtgtc
     2761 gatccccagc agagaagggc ggtccgggca ctgcttgtgc cataactctt cacgcattca
     2821 agggcgtcaa tctcaccctt caatgcctca tattttctct gccttttttc tgtcatcaac
     2881 cgcaccatgt cagctttttc cgtggtaatc ccatcctcat catgggcata gctgttcacc
     2941 ttgatcgcgg catccttcag gggtggaaac tggaggggag agatcccgtt gtcagttatc
     3001 gaatatctcc tgtattgccc ggaaataccg gattgttgaa ggtcactggt ctcggggggc
     3061 ccgattgctt tctcatcgag gctgtaggtg ccctcgcaga gggtcttgtc acagaggatg
     3121 actgccggga cctggcactt ccatgcgagg gagagggcaa tcgaggacca gacatacgcc
     3181 tgctcggcgt ccccgggcgc cacgatgagc cgtgggaact ctccctgccc tgcatggagg
     3241 acgaagttga ggtctgtctg cccggtatag gtgggaaggc cggtggaagg cccggtccgt
     3301 tgccccatca ccaccaccac aggaacctct gatgacccgg aaaatgagaa cccttccgtc
     3361 atcaggcaga agccgcctcc cgaggtgccg accgcagtct tctttcccgc gtaggaaaac
     3421 ccgagcgcca tcagcatgac ggctatctcg ttctcgggat ggaccacggt cagggcgaac
     3481 tggccggcta cccctgccag gaaatggagc aggttcgagg tgggggtcat gggataggcc
     3541 acgaaggcat cgagcccgcc gtgcaggagt cccaggccga tcgcctcgtt tccggtgaga
     3601 agcgggcagc aggtgtggcc tgcccgtggg atgacctttt gttcccggac tgcgtcatat
     3661 cccttcctgg caataaccag gttcttttcc gggtcttttg ggacctgccg ccggagaacg
     3721 gtctcgacca cctcgaaggg gatgccggcc gcctttgcga aggccccgat gatgcacgtg
     3781 ttccccatca ctgcggaggc gccctcggct ttcagtatct cttcgaccgg tatccccagg
     3841 cccttgccat ggacccggcc gctgtcatag agcacgaccg tgtcatcgtg gatgaggtcc
     3901 cggtgcagat cgagagtgtc ctgggagagg gcgagcagat aatcgcaccg gtcccggtg
//
LOCUS       DAOOCO010000172         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21722_length_3952_cov_4.628176, whole genome shotgun sequence.
ACCESSION   DAOOCO010000172 DAOOCO010000000
VERSION     DAOOCO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21722_length_3952_cov_4.628176"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..778)
                     /locus_tag="PK154_08860"
     CDS             complement(209..778)
                     /locus_tag="PK154_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HPW11207.1"
                     /translation="MGLLREGINEVIATTCRNAAPMGIIYRNGVARMVVFWGSHTAER
                     IREHGWVVANFLYDPVVYVKTAFEDLPPEDFIDEQVGEIAVQRLSGVEAWAAYQAEID
                     QETNEALIVTLTLLKETICSLALHPVNRGFNSIIDATVHATRFQQSRDPHLHDLIRHH
                     ASLVRKCGGARELQALELLSTYLDFGDEE"
     gene            complement(766..1572)
                     /locus_tag="PK154_08865"
     CDS             complement(766..1572)
                     /locus_tag="PK154_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HPW11208.1"
                     /translation="MTRADRAQLAMMLEVCAFPKPGNVDRCHDYAETRLEHFLASTIL
                     SRKALERAEQCEGGIGDIILDAVGRTNVHSGGNTHFGAFILLIPLLCGGDIPGACRII
                     SETTVEDAILFYRAFGKTRVRVLKDDELDVNDPRATDELRERRMTLYDVMLYSAKHDM
                     VAREWINGFALTRRGADLLKSHHGGCGAVVGTFMDLLSTEPDTFIIKKHGRDVAWEVM
                     RKAREVREGIRDIARFDQELIDSGINPGSIADIIIASLYIALGEGWQWDY"
     gene            complement(1559..2701)
                     /locus_tag="PK154_08870"
     CDS             complement(1559..2701)
                     /locus_tag="PK154_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HPW11209.1"
                     /translation="MENNEYFRRFGIILNRRVVKTPLAIASMAGIVDATYVLEREAHV
                     GAAFIGGYSIDEKTLAASHEIAAKGRKEFLCDDPLKEIAEQVRQLRRSDVVTGVNMRG
                     SRPDSFREAARQLGDEVIYEIDAHCRQEPMKQAGVGEYYLHHPDELVLCVQTLKSEGV
                     VVSVKIRAGIHSNDAALAQLLWKAGADILHVDLMDFGYQKIRQMRNNCPIFIIANNSM
                     HTFERMKEMLSHGADMVSLARKADERTLGGLDAAISRYADEHGWYNSPKQLCRGGDLR
                     SLAFCCMPVKNCPLIPTLEKAGVTREEYMKLKQEYVKDTPLAEGKQTCFGSLAWCCKD
                     SSPCMFRDLTIRQAGLTNSEYMRKKRELSEKLMHWLFYERATDDTC"
     gene            2943..3344
                     /gene="cfbA"
                     /locus_tag="PK154_08875"
     CDS             2943..3344
                     /gene="cfbA"
                     /locus_tag="PK154_08875"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HPW11210.1"
                     /translation="MSKKGILLVGHGSKLPYNKELVEETAGLIARRYPDFIVKCGFMN
                     MNSPSIKDSMDAFRNEPIEVLVVVPLFLAKGVHILQDIPEIIGLSGGETKGQFAMNGK
                     NIPLLYADPIGSDPLLADLMIKNAERVLSTL"
     gene            3351..>3952
                     /locus_tag="PK154_08880"
     CDS             3351..>3952
                     /locus_tag="PK154_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HPW11211.1"
                     /translation="MRILVIDTIHGGLDIAGYLNGRGHFTDVVDVYRGKAGIDAATAA
                     ERSYDLAIAPVHLDPWHPLLRSLTVPVISHHDAVRWIIGKDRPAPFIEITGARGKTTT
                     ACALAHVMKGPGILHTSIGTYRFPEKELLWKKSITPSSLVPAAEEALRIGGWCISEVS
                     LGCTGAGEYGIITSSEDYLFAGGKRVILGCRGVWEHHLIGG"
BASE COUNT          862 a         1096 c         1078 g          916 t
ORIGIN      
        1 tatgcttgtg gccgtcaaag gaatcatttt ttttttggac tacgggctca cctggcctga
       61 acagggatca gaatgccggc agtcccaagc caggaaccgt gacattgcat cagaggtgct
      121 ggggttgctt tcccgggtct cctggtgttt caaacctctg ttgtatcaaa cagaaaagag
      181 ccgggagaat aggtggtgag gtgcaggatt attcttcgtc cccgaaatcc agataggtag
      241 agaggagctc gagcgcttgc agctcgcgcg caccgccgca tttcctgaca agtgaggcat
      301 ggtgccggat caggtcgtgg agatgggggt cgcgggattg ctggaaccgg gtcgcgtgaa
      361 cggtcgcatc gatgatactg ttgaaaccac gattgaccgg gtgcagggct aggctgcaga
      421 tcgtctcttt caggagagtg agggtgacga tcagagcctc attggtttcc tggtcaatct
      481 cggcctggta ggctgcccag gcttccaccc cggaaagtcg ctggacggca atctccccca
      541 cctgttcatc gataaaatct tccggcggca gatcttcaaa ggctgttttc acatagacca
      601 ccgggtcgta gagaaaattt gccacgaccc agccgtgctc ccttatccgc tcggcagtat
      661 gacttcccca gaataccacc atccgcgcca ctccgttccg gtagatgatg cccatgggtg
      721 ccgcattccg gcaggtggtg gcgataacct cgttgatccc ctctctcaat agtcccattg
      781 ccatccttca cccagagcga tatagaggga ggcaatgata atatctgcga ttgagccggg
      841 atttatgccg ctatcgatca attcctggtc gaaccgggcg atgtcgcgga ttccttccct
      901 tacctcccgt gcctttctca tgacctccca ggcgacgtct cggccatgct tcttgatgat
      961 gaacgtatca ggttcggtgg acaggagatc catgaacgtt cccaccacag ccccgcatcc
     1021 cccatgatgg gatttgagga gatcggcccc tcgccgggta agggcaaatc cgttgatcca
     1081 ttcacgggca accatgtcat gtttggcaga atacagcatg acatcgtata acgtcatccg
     1141 gcgttctctg agttcatcgg tagcgcgtgg atcgttcaca tcaagctcat cgtccttcaa
     1201 caccctgacc cgcgtttttc caaaagcccg gtaaaagagg atagcatctt cgacagtagt
     1261 ctctgatatg atacggcagg caccggggat atcccccccg cagagaaggg ggatcagcag
     1321 gataaaagcc ccgaaatggg tgttcccccc cgaatggacg ttggttcgtc cgactgcatc
     1381 gaggatgatg tccccgatcc ccccttcgca ctgctccgcc ctttcgagcg ctttgcgtga
     1441 aaggatggtc gatgcaagga aatgttcgag ccgggtctcg gcatagtcat ggcaccggtc
     1501 cacgtttccc ggtttgggga aagcgcagac ctcgagcatc atcgcaagtt gcgcacggtc
     1561 agcacgtgtc atcggttgcc cgttcataaa acaaccagtg catcagtttc tctgaaagtt
     1621 cccgtttctt ccgcatgtac tcgctgttgg tcaggcctgc ctgccgtatc gtgagatcgc
     1681 ggaacatgca gggagaggaa tccttgcagc accaggccag gctgccaaaa caggtctgtt
     1741 tcccctcggc caggggggta tctttgacgt attcctgctt cagcttcatg tattcttccc
     1801 ttgtcacccc tgccttttcc agggtcggta taagggggca gttcttgacc ggcatgcagc
     1861 agaacgcgag ggaacggaga tccccacccc tgcagagctg ttttggggag ttgtaccagc
     1921 catgctcatc agcataccgg gatatggcag catcgaggcc tccaagcgtc cgctcgtcgg
     1981 ctttccgcgc gagcgagacc atgtctgcac catgggagag catctctttc atccgttcaa
     2041 aggtatgcat ggaattgttt gcgatgatga agatggggca gttgttccgc atctgccgga
     2101 tcttctggta tccaaagtcc atcaggtcga catgaaggat atcggcgccg gctttccaga
     2161 gaagctgtgc cagagctgca tcgttggaat gtatccctgc ccggatcttc acagagacaa
     2221 cgaccccttc ggacttgagc gtctgtacgc acaggacaag ttcatcagga tgatggaggt
     2281 aatattcacc gaccccggcc tgtttcatcg gctcctggcg gcagtgggca tcgatctcat
     2341 agatcacctc gtctccgagc tgccgggcgg cctcccggaa cgagtcaggg cggctccccc
     2401 tcatattgac tccggtcaca acatcactcc tgcggagctg ccggacctgc tcagcgatct
     2461 ccttgagcgg gtcatcacag aggaactcct ttctcccttt tgccgcgatc tcatggctgg
     2521 cagcgagcgt cttttcatcg atggagtatc ccccgataaa ggcagctccg acatgcgcct
     2581 cccgctcgag cacatacgtg gcgtccacga ttccggccat tgatgctatg gcaaggggag
     2641 tctttaccac cctacgattg agaatgattc caaaccttcg gaaatattcg ttattttcca
     2701 tatgtaacac gctggacagg tatcaaaatt acaccgaatg cagtagatga tccattgtat
     2761 gggattataa atcgtttgaa taccttaaaa ataaaccgga aggattatac aaaaaatggt
     2821 ataaaaatcc tttcttcccc ccctccagtg cctgcggatt cctgcatttt gaggtgctgc
     2881 ctggaatgtt cggggggaaa attggcaatt gttttacgtc catcagcaca acgtattacc
     2941 atatgagtaa gaagggcatc ctccttgtgg gccatggaag taaactcccg tataataagg
     3001 aacttgtcga agagactgcc gggcttattg ccaggcgata tccggatttt atcgtaaaat
     3061 gcggatttat gaacatgaac tccccctcca tcaaggactc catggatgcg ttccggaatg
     3121 agccgatcga agtgctggtt gtcgtgccgc tgttccttgc caaaggggtg catatcctgc
     3181 aggatatccc ggagattata gggctatcgg gcggggagac aaaggggcag tttgccatga
     3241 acggaaagaa catccccctc ctctatgctg atcccatcgg cagcgacccg cttcttgctg
     3301 acctgatgat aaagaatgcc gaacgggtgc tctcaaccct gtaatccttc atgcgaattc
     3361 ttgtcatcga cacgatccat ggcggtcttg acatcgcagg atacctgaac ggcaggggcc
     3421 atttcaccga cgtggtcgat gtttaccgag gaaaggcggg gatagatgcc gcaaccgcag
     3481 cagagcggtc gtatgacctg gcaattgcac cggttcacct cgatccatgg cacccgctgc
     3541 tccggtcgct tacggtcccc gtcatctcac accacgatgc ggtaaggtgg atcatcggaa
     3601 aggacaggcc tgcaccgttc atcgagatca cgggggccag gggcaagacg accactgcct
     3661 gtgcacttgc ccatgtcatg aaggggccgg gcatcctcca tacttccatc ggaacctacc
     3721 gtttcccgga aaaggagctt ctctggaaga agagtattac cccctcctcc cttgtcccgg
     3781 cggcagaaga ggcgctgcgt atcgggggtt ggtgcatcag cgaggtctcc ctcgggtgca
     3841 cgggtgcagg ggagtatggg atcatcacct catcggagga ttaccttttc gccggtggaa
     3901 aaagggtaat cctcgggtgc aggggagtat gggagcatca cctcatcgga gg
//
LOCUS       DAOOCO010000173         3925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_21923_length_3925_cov_3.458656, whole genome shotgun sequence.
ACCESSION   DAOOCO010000173 DAOOCO010000000
VERSION     DAOOCO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21923_length_3925_cov_3.458656"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1884)
                     /gene="groL"
                     /locus_tag="PK154_08885"
     CDS             complement(271..1884)
                     /gene="groL"
                     /locus_tag="PK154_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106636.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW11212.1"
                     /translation="MATSKQLMFDEEARKALLSGVNKVADTVKITLGPKGRYVVIDKS
                     MNPIVTNDGVTIAKEISLHDKFENIGAKLVKEVAQKTQDKTGDGTTTATLLAQAILTE
                     GLKNITSGANPIEIKRGIDAAIADVVRYIGSTSVPVKGREGILQVATVSANNDEEIGK
                     LISDAMEKVGYGGLITVEDAKSLETSLDVVQGMQFDRGFISPYMVTDHEKMTCEYEDP
                     NILITDRKITSLKQMIPVLEMASQEGKPLLIIAEDVEGEALAALILNIIRGSFKVCAV
                     KAPGYGEERKAILEDIAIITGATVISEERGMKLENVSRAALGSAHTVKTDGEKTLIVE
                     GKGDRKALDDRMHLIESQINITDSEFRKEELKKRLGNLSGGVAVIKVGAATETELKEK
                     KMRIDDALNATKAAVEEGVVTGGGVTLFRAIGSLDRLSFDDDRKIGVAIVRRALEEPL
                     RQIAKNAGVEGAEVIATIKGHEENGFGYNAKTGAYENLMEHGVIDPAKVVRIGLQNAG
                     SIAGMILSTEIAITDFDEEKDTQSAAIII"
     gene            complement(1896..2171)
                     /gene="groES"
                     /locus_tag="PK154_08890"
     CDS             complement(1896..2171)
                     /gene="groES"
                     /locus_tag="PK154_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPW11213.1"
                     /translation="MKEIIPIGERVLIKPLKMEEMTRSGIYIPESAQEHRKEGSVIAV
                     GSRDDGSALPLKPGDHVLYGGYSADKFEMDGENYVFVTFKDILAKIE"
     gene            2311..2715
                     /locus_tag="PK154_08895"
     CDS             2311..2715
                     /locus_tag="PK154_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW11214.1"
                     /translation="MRQILVKQIERPLLRDEDEDLQWFCNSLGIGEGRDVERVATRVL
                     FTLLDHQPHETGLPVDVIAQDLAVSSSRINHHIRNLVDAGAVYRHKRLIYLRGNSLRS
                     MVQEIRKDVLRMLDDLEVAATEIDQAFGIVQR"
     gene            2738..3208
                     /locus_tag="PK154_08900"
     CDS             2738..3208
                     /locus_tag="PK154_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HPW11215.1"
                     /translation="MTVSSYYGIHDIFRRLLENIQDIIVIALCLLLFALMIKSLMGMF
                     YEFLEPFNFQYIASELIYILVLVEIYRLLIIYLREHRIAVDIMIEVGIVSILREVILH
                     GILGIDPFILVAIAILLISLTLLLRYGSLRRDEKEPETRETVFKELKKSFAKGE"
     gene            3370..3900
                     /locus_tag="PK154_08905"
     CDS             3370..3900
                     /locus_tag="PK154_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPW11216.1"
                     /translation="MDLFKKKKGTEVSPATPGTSLEPRTRTPGFVRDFDSIFDDFRRS
                     VDSLMRPYFPLDFPRDLSEWQMFQYAPLDLIDEGDHYKVHVELPGLSKEDVEVDLNNE
                     SLSIQGQRKEETEKKEKNYLHRERYYSGFKRSIDFPEEVDPGKADGSMNNGVLELTIP
                     KKEPRPEERRRKIEIK"
BASE COUNT          923 a         1007 c          925 g         1070 t
ORIGIN      
        1 cgcgattata ttctctgttt gacaaaaagg tcggtggcta gattgtcact atacactaat
       61 atcgtttaat ggtggtttaa ttcggaaaat aaacacaaaa aagaggtcta agctcaagcc
      121 cgattggcaa cggagcccgg aaataagtta tttctctgct cgcagaaatg cgggtgaaaa
      181 aggtaatgga ggtttttata gggaaatgac agtccggcaa aaatccagtt tccagggaat
      241 gcaagatctc tgccgccttg tccttgtaat tcagatgata atggccgcac tctgcgtatc
      301 tttctcctca tcgaaatcgg taatggcaat ttcggtggaa aggatcatgc ctgcaattga
      361 ccctgcattc tgaagcccaa tcctgactac cttggcagga tcgataaccc catgctccat
      421 gaggttttcg taggcgccgg tctttgcgtt atacccaaat ccattttctt cgtgcccctt
      481 gatagtggcg atgacttctg caccctcaac accggcattc tttgcaatct gcctcaatgg
      541 ctcttctaat gcacgcctaa caatagcgac accgatcttc cggtcatcat cgaaggataa
      601 cctgtcgaga gacccgatgg cacggaagag tgtaacaccg cccccagtga ccactccttc
      661 ttctacggct gccttcgtgg cattcagcgc atcatcaatg cgcattttct tctccttcaa
      721 ttcagtctcg gttgctgcac ccaccttgat aaccgcaaca ccgccactga ggtttccaag
      781 tcgctttttc agttcctctt tcctgaactc ggaatctgtg atatttatct gcgactcgat
      841 caggtgcatc cggtcatcga gtgctttccg gtcacctttt ccttcgacaa taagggtctt
      901 ctctccatct gtcttgacgg tgtgagcgga tcccagtgca gcacgagata cattttcaag
      961 tttcatgcca cgctcttctg aaatcaccgt cgctccggta atgattgcaa tatcttcgag
     1021 gatagctttt cgctcctctc catatccagg tgccttgaca gcacagacct tgaaggaccc
     1081 gcggatgata ttcaggatga gtgcagccag ggcttcgcct tcaacatctt ccgcaatgat
     1141 aaggagcggt tttccttcct gggaggccat ttcgaggacg gggatcatct gtttaagaga
     1201 agtgattttt ctgtcggtaa tcaggatgtt ggggtcctca tactcgcagg tcatcttctc
     1261 atgatccgtt accatatacg gagagatgaa tccgcgatca aactgcatcc cctgtacgac
     1321 atcgaggctg gtttcaaggc tttttgcatc ctcgaccgtg atgagaccgc cataacctac
     1381 cttctccatg gcgtccgaga tgagcttccc aatctcttca tcgttattgg ccgatactgt
     1441 cgcaacctga agaatcccct ctcttccctt gaccggtaca ctggtcgaac cgatgtacct
     1501 aacaacgtca gcaatcgccg catcgatgcc ccgcttgatc tctatcgggt tggcccccga
     1561 ggtaatattc ttcaggcctt cggtgagaat agcctgggca agaagagttg ccgttgttgt
     1621 cccatcaccg gtcttgtcct gggtcttctg ggcaacctct ttgaccagct tcgcgccgat
     1681 gttctcgaac ttgtcgtgaa gggagatctc ttttgcgatc gtcactccat cgtttgtcac
     1741 gatggggttc atggacttgt ctatcaccac ataccgccct ttcgggccaa gcgtgatctt
     1801 gaccgtgtcc gctactttgt tgacacctga gagaagagct ttccgggcct cttcgtcaaa
     1861 catcagctgt ttggatgtcg ccataatcag ccaccctatt caattttcgc cagaatgtcc
     1921 ttgaaggtaa caaaaacgta attttctccg tccatctcga atttatccgc actgtatccc
     1981 ccatacagca catgatctcc cggcttcagg ggaagtgcag aaccatcatc ccgactccct
     2041 accgcgatga cggatccttc ttttcgatgt tcctgggcgg actctggaat gtagatgcca
     2101 cttcttgtca tctcttccat tttcaggggt tttatcagaa ccctttctcc gatgggaata
     2161 atttccttca ctcatatgca cctctggaaa ttattgcatt ctattggttt agtatcaaat
     2221 atatttaaaa attatcaata atgttgatat catttgaata ttgttgcaaa cttatttatc
     2281 ctgcggagag agattttgta atcggttacc atgaggcaga tcctggtcaa gcagattgag
     2341 cggccgctac tccgggatga ggatgaagat ctccagtggt tctgtaacag cctcggtatc
     2401 ggcgagggga gggacgtgga gcgggtcgct acccgtgtcc tcttcaccct gctcgaccac
     2461 cagccccatg aaacgggctt gcccgtggat gttatcgccc aggatctcgc cgtctccagc
     2521 tcgcggatca atcatcatat caggaacctc gtggacgcag gggcggtata ccgccacaag
     2581 cggttgattt acctccgggg aaacagcctc cgttccatgg tccaggagat ccggaaagat
     2641 gttctccgga tgcttgacga cctcgaagtg gcggcaactg aaatcgacca ggcgtttgga
     2701 atcgtgcaaa ggtaatggat agcagggagg aatagggatg accgtttcgt catactatgg
     2761 gatccatgat atctttcgac gattgcttga gaatattcag gatatcatcg tcatcgccct
     2821 atgcttattg ctttttgcgc tcatgatcaa gtcgttaatg gggatgttct atgaattcct
     2881 ggagccgttc aatttccagt atattgcttc ggagctgatc tatatcctcg tactggtcga
     2941 gatataccgg ctcctgatca tctacctcag ggagcatcgt attgccgtgg atattatgat
     3001 tgaggtggga attgtctcca tcctcaggga ggtcatcctc catggtatcc tgggcatcga
     3061 tcccttcata ctcgtcgcga ttgcaatcct tcttatctca ttgacgttac tgctccgata
     3121 cggatcgctg cggcgtgatg agaaggaacc agagacccgg gagacggtat tcaaggaact
     3181 gaaaaaatcg ttcgccaaag gggaatgatg tgtcctatca ttctttcgca ggagtacccc
     3241 gatgaagggg gatctccccc acctattcca tttttcccca cggcttttcc ttgcgaaccg
     3301 gatttgccta ataaattagg tttatatttc attaatgcta accgtcagtt agtataggag
     3361 ggaacaccta tggatctctt caaaaagaaa aaaggtacag aagtgtctcc tgctacacca
     3421 ggcacttctc ttgaaccgcg gacccggaca cctggattcg tcagggactt tgactcgatc
     3481 tttgatgatt tcaggagatc tgtggacagt ctcatgcggc cgtacttccc gctggacttc
     3541 ccccgtgatc tctccgaatg gcagatgttc cagtatgcgc cattggatct catcgatgaa
     3601 ggagatcact ataaggtcca tgtggagctc cccgggctgt ccaaagagga tgtcgaagtg
     3661 gacctcaaca acgagagtct ctctatccag ggccagagaa aggaagaaac cgagaagaaa
     3721 gagaagaatt atcttcaccg ggagcggtat tattccggct tcaaaaggag tatcgatttt
     3781 cccgaggagg tcgatccggg taaggcagat ggatcgatga acaacggggt gctcgaactg
     3841 acaatcccca aaaaggaacc gcgacctgaa gaaaggcgcc ggaaaatcga gattaagtaa
     3901 tttatcactt ttttcgggat gggta
//
LOCUS       DAOOCO010000174         3911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_22042_length_3911_cov_4.729253, whole genome shotgun sequence.
ACCESSION   DAOOCO010000174 DAOOCO010000000
VERSION     DAOOCO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22042_length_3911_cov_4.729253"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK154_08910"
     CDS             <1..248
                     /locus_tag="PK154_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11217.1"
                     /translation="PPCADYPCKRVRLMDKRYRTRYHMSVIRNLEEIRAHGIRQFVTN
                     ERERWTCRTCGGVIDVHHGRCASCGKEREASGCDKEE"
     gene            553..759
                     /locus_tag="PK154_08915"
     CDS             553..759
                     /locus_tag="PK154_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11218.1"
                     /translation="MMHRHGPGMMHWRGMLMHEIMEKIPDDQKKVILKRIIDGKILLR
                     ENRIKQMQFKIETMKMIRKLIDEC"
     gene            complement(907..1332)
                     /locus_tag="PK154_08920"
     CDS             complement(907..1332)
                     /locus_tag="PK154_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11219.1"
                     /translation="MMEIQYREIGEAIGISGTRQEELLGHLYERITGGDAGSFDPFQC
                     MMADDRDPQEETISILRWVLKEPSLSPEERIFLAYQTGAFLPLQSEMDDTDEMTIDEE
                     DQEIRALLLSFLQSEEGQDMFHPIIDDLEGEDDLENEGI"
     gene            1521..2021
                     /locus_tag="PK154_08925"
     CDS             1521..2021
                     /locus_tag="PK154_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11220.1"
                     /translation="MIPFHEAMDEYRSQMEHGVIRQAYRGLMEYIRDLRVHFETTVPG
                     SFVSGSIYPGYMDMTYFSFTPESLHRRKLKIAVVFLHETGRFEAWLAGMNKRIQAEYW
                     NRLKESNWSAYPLAASLQGVDAITTHVLSENPDFRDLPLLTRHIEQGVMQFTADIEEF
                     LSIEQD"
     gene            2131..3783
                     /locus_tag="PK154_08930"
     CDS             2131..3783
                     /locus_tag="PK154_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW11221.1"
                     /translation="MIRALLVDRSGSGDLFRWSEGGRDLAVSLLSDPAGLPDALAGKE
                     YDIILLSLHSGDSATIETLRQIRIQDPRIPCVVISDVLDSDLGFAALTAGADTYHWDI
                     MKETFQKVLVPVIRNLVQHRRDEDQVLQDNLFMRTIHDASPVAACVIQDHKILWVNAI
                     VPRKLGYTQEELIGSDLVRLFPNQAEHRRVDSGLLQTSNKEGWGVAEGLLKRKDGTLI
                     HCHLLSRPIDPADPGQGQIIIGQDITDYVRIRDLLRKSEVRYQDLLDSANSIVLRVSP
                     EGSIRFINNSGAAFFGYAPEELMGKNLIDTIVPRRSITGRDLEGMIRDVMHAPEDYEV
                     NVNENMKQNGERVWVAWTNRAIRDDRGKLTEMVSIGHDITDRKINQDDRGKGSTPWIS
                     AFLEGTDIREEVFEEVYTLSLELSREGREGKQIGTTFLIGSAPEVLARSTQLILNPFE
                     GHPLSVRNVATSEIRETIKELSQIDGAFVISGDGIIEAAGRYITIDTSQASIPKGLGT
                     RHASVAGLTLETPAVGIVLSQSGGKVSLFKDGRIFRVIAVKD"
BASE COUNT          909 a         1043 c         1085 g          874 t
ORIGIN      
        1 gcccaccctg tgccgactat ccctgtaaac gggtacggct gatggacaag cggtaccgga
       61 cacggtatca catgagcgtt atcaggaacc tcgaggagat cagggcccat gggatccggc
      121 agtttgtcac caatgagcgg gagcgctgga catgcaggac ctgtggcgga gtgatcgacg
      181 tccatcatgg ccggtgtgca tcctgcggga aggaacggga ggcatcgggc tgcgataagg
      241 aggagtgagc cattcgttct tttcagggaa ttcatcatat cattttggcc cattgagaaa
      301 ggggaaggtg tactatcagc ccgaaaatga ggtctgtgca atgatatcct tcctgcaaat
      361 gggatttttt ttttgttcac cgaaggtctt ttggatgcca ataccggtgt ggaatcccca
      421 atagcccttc ctttggagcc acttctgcca ggagatgctc acatttttta aaaacgggga
      481 gaaggttatt aaaatatgtt gtggtaggta cccacgttcg atacatcgga caagttaatg
      541 agttgatgga acatgatgca ccgacacgga cctggcatga tgcactggcg gggcatgctc
      601 atgcatgaaa taatggagaa gatccctgac gaccagaaga aagtgatcct gaaacgtatc
      661 atcgatggca aaatcctctt gagggagaac cgcatcaagc agatgcagtt caagatcgag
      721 accatgaaaa tgatcaggaa actgatcgac gagtgctgat tgaagagcgc ttactcacct
      781 ctctcttttt tctcctggcc ggaacaggtt tctttgcccc ccctctccaa tgggaaggcg
      841 aatccctgtc aggtgcggta atccccgtgt ttgagaccat atccctgttc tccccgtccc
      901 ctcctctcag ataccctcat tctccaggtc atcctccccc tcaaggtcgt cgatgatagg
      961 atggaacata tcctgcccct cctcgctctg gagaaaggaa agcagtaacg cccggatctc
     1021 ctgatcttcc tcatcgatgg tcatctcatc agtatcgtcc atctcgctct gcagagggag
     1081 gaatgcaccg gtctgatacg caagaaaaat ccgttcttcc ggtgacaggg acggttcttt
     1141 aagaacccat ctcaggatcg atatcgtctc ttcctgagga tctctatcgt cagccatcat
     1201 gcactggaag ggatcgaacg atcccgcatc tccgccagtt atgcgttcat aaaggtgtcc
     1261 gagcagttcc tcctgccggg tgccggaaat ccctatcgcc tcgccaattt cgcggtattg
     1321 tatctccatc atgggtgatc atctcacgac aatcccttaa ccattccgtt tccccccgat
     1381 gaaaacagcc tgatggaagg tttgggatcg ttatacttcc cctctgtaca ttccccccgg
     1441 ttttttggat gattaaagat aacgacaatt gtcatgatga gggatgagag agagggttat
     1501 actggtacga ggagctgaga atgataccgt ttcatgaagc catggatgaa taccggagcc
     1561 agatggaaca tggggtgatc aggcaggcat accggggctt gatggaatac atcagggacc
     1621 tgcgggtgca ttttgaaacc accgttcctg gctccttcgt ctccggaagt atctatcccg
     1681 gatacatgga tatgacctac ttctctttca ccccggagtc tttgcaccgt cggaaactga
     1741 agatagccgt cgtgtttctc catgagacgg gccggttcga ggcctggctg gcagggatga
     1801 acaaacgaat ccaggcggaa tactggaacc ggttgaagga gagcaactgg agcgcatacc
     1861 ctctcgccgc ttccctgcag ggggtcgatg caatcaccac tcacgttctt tcagagaatc
     1921 cggactttcg tgatctgcct ctcctgacgc ggcatatcga gcagggagtc atgcaattta
     1981 ctgccgatat tgaagaattc ctctcaatag aacaagattg agaagtaaat tccctctcat
     2041 ggagcagcac ccgagtagtt cgctcttcct cgcatcgtta ccgacgggga ctccaatacc
     2101 ttgatgtggg atccctggag agaactatct atgatcaggg cgctcctggt ggatcgttcc
     2161 ggcagcgggg atctcttccg ctggtctgag ggcggcaggg accttgccgt ctccctgctg
     2221 tctgatcctg ccggactccc tgatgccctg gccggaaagg aatacgatat catccttctc
     2281 tctctgcatt ccggtgattc tgccacaatc gagacccttc ggcagatccg tatccaggat
     2341 cccaggatcc cctgtgtcgt catctctgat gtgctcgaca gcgacctcgg gtttgccgca
     2401 ctcactgcag gggcagatac ctatcattgg gacatcatga aagagacgtt ccagaaggtc
     2461 ctggtgccgg tcatcaggaa cctggtgcag catcgaagag acgaggacca ggtactccag
     2521 gacaatctct tcatgcgtac gatccatgat gcctcaccgg ttgccgcatg cgtcattcaa
     2581 gatcacaaga tcctctgggt gaacgcgatc gtcccgagaa aactcgggta tacccaggaa
     2641 gagctgattg ggtctgacct ggtccgcctg ttccccaacc aggcagagca tcgccgagtc
     2701 gactccgggc tccttcagac ttcgaataaa gagggatggg gcgtcgccga gggtctgttg
     2761 aagcggaaag acggcacact catccactgc cacctcctct cccggccaat tgatccggct
     2821 gatcccggcc aaggccagat catcatcggc caggatatca cggattatgt ccgaatacgc
     2881 gatctcctcc ggaaaagcga ggtcaggtac caggatctgc ttgacagcgc aaacagcatt
     2941 gtgctccgcg tcagtccgga ggggagcatc cggttcatca acaattccgg agcagccttt
     3001 tttggctatg ctcctgaaga actcatggga aagaatctca tcgataccat tgttccaagg
     3061 agatcaatta ccggccgtga cctcgaaggg atgatccggg atgtcatgca tgcccctgaa
     3121 gactacgagg tcaacgtgaa cgagaacatg aaacagaacg gtgaacgggt ctgggtggca
     3181 tggaccaacc gtgcaatccg tgacgatcgg gggaagctca ccgagatggt ctccatcggc
     3241 catgatatca ccgaccggaa gatcaaccag gatgaccgtg gaaagggatc gacgccctgg
     3301 atcagtgcgt tccttgaagg gacggatatc cgcgaagagg tatttgagga ggtgtacacg
     3361 ctctcgcttg aactatcacg ggaggggagg gaaggcaagc agatcgggac gacgttttta
     3421 atcgggagcg ctcccgaggt gctggcccgg tccacccagc tgatcctcaa tcctttcgag
     3481 gggcacccat tgtcggtacg gaatgtggcc acgagcgaga tccgggagac catcaaggag
     3541 ctctcccaga ttgacggggc gttcgtcatc tccggtgacg ggatcatcga ggctgcaggg
     3601 cggtacatca cgatcgatac cagccaggcc tcgatcccga aggggctcgg cacccgtcat
     3661 gcatcggtgg caggcctcac gctggagacc ccggcagtgg ggatcgtcct ctcccagagc
     3721 ggggggaagg tctcgctctt caaggatggg cggatcttcc gggtgatcgc ggtaaaagat
     3781 taaaaagggg ggggttcaaa atgccctcag cgggcaggga ctagtggcgg tgtcacacct
     3841 gggattcatg gcccccgtgc cctttcacat tcaggtaaag gtggtgggca tagacgacga
     3901 agttgaggta g
//
LOCUS       DAOOCO010000175         3808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_22905_length_3808_cov_4.688249, whole genome shotgun sequence.
ACCESSION   DAOOCO010000175 DAOOCO010000000
VERSION     DAOOCO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22905_length_3808_cov_4.688249"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..628
                     /locus_tag="PK154_08935"
     CDS             38..628
                     /locus_tag="PK154_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HPW11222.1"
                     /translation="MAKSMYAYVREAWKKPAETGVKELQWQRMQEWRREESVVRLEHP
                     TRIDRARNLGYKAKQGIAVVRVRIRRGGRRKPRYVRARRSARMGVNRMTAGKSLQRIA
                     EERASVKYPNMEVLNSYWVGQDGKQKWFEVILVDGNHPSIKADRNLAWLNNPVHRGRA
                     ERGKTSAGRKGRGMMNRGKGTEKTRPSIRSHGNLGK"
     gene            659..1300
                     /locus_tag="PK154_08940"
     CDS             659..1300
                     /locus_tag="PK154_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HPW11223.1"
                     /translation="MMETDACIHPYPVGTASVRRMAMEAGALGFGSIVVPGEVAGVYH
                     GVRIIPAVIISETDLKRVIGQIRRHANSGNLVFVDAGDNGFNRAVLAQKGLNAIRCLH
                     QIPKKAFDHISARLAYENSIAVEIDLYPLIHEKGIFRQRVLNRYNDLMRLHSRYHFPL
                     ILSSNACSVLDLRSPDEIRLLCSLFGMEKDDTDGALQTISTLISPVESVMVAP"
     gene            1297..1785
                     /locus_tag="PK154_08945"
     CDS             1297..1785
                     /locus_tag="PK154_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HPW11224.1"
                     /translation="MKPRPPTLREKKRYLLLRIDPPTPDPDPKDLYFSVLDSVTSLYG
                     DAEAARMMPVVISCGSGYAIVRCIRGYEEKLRIAIVTLTSVTERRIALHAVATSGTLL
                     ALRRRVSGLSPVRVETISSLHFRDREYTARRYQGQKVDLQEKGINRQELLFLTQDDLE
                     GL"
     gene            1785..2510
                     /gene="psmA"
                     /locus_tag="PK154_08950"
     CDS             1785..2510
                     /gene="psmA"
                     /locus_tag="PK154_08950"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HPW11225.1"
                     /translation="MQPQAYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKC
                     EDGIVLIVDKRVSSRLLEASSIEKIFMIDEHIGVASSGLVGDARALVDRARIECQINR
                     VTYDEKMDVEALAKKLCDHMQTYTQFGGARPYGTALLIAGISDGRCRLFETDPSGTLL
                     EYKATGIGIGRNAVMKVFEEEYSHEAPLKDSILLGLKALHDATEGKFDVNTVEIGIIE
                     TKTPQFRKMTKEEVASFVEQFEK"
     gene            2519..3223
                     /locus_tag="PK154_08955"
     CDS             2519..3223
                     /locus_tag="PK154_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HPW11226.1"
                     /translation="MIPLDRAVVARLESHGEKFELLVDPDRAVKFRQGEPVDLEDMVA
                     ALAIFENSSRGTRASEEALNKVFHTTEFLPVATRIIQKGEIHLTAEQRRHMIAEKRRQ
                     VITFIARNAVNPQTGLPHPPQRIELAMDEARVNIDLYKSVDEQVKETVKALRPLLPIR
                     FEEIRIAVKIPPDFASKAFGEIQPVAQIEKDEWQKDGSWICVVRIPAGIQGDFYDLIN
                     RITKGQGEVRILNQVY"
     gene            3268..3558
                     /locus_tag="PK154_08960"
     CDS             3268..3558
                     /locus_tag="PK154_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HPW11227.1"
                     /translation="MASRTQKAKGKVSGSAGRFGPRYGRFVRKRVSDMEQVSKAAHRC
                     PRCDTIAVRRQGTGIWGCRKCGFKFAGGAYQPQTPQLRIALRTIDRSLGKEA"
     gene            3565..3699
                     /locus_tag="PK154_08965"
     CDS             3565..3699
                     /locus_tag="PK154_08965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HPW11228.1"
                     /translation="MAGSYKCARCKHKVEIDVNVRCPYCGHRILFKERGAAIKELKAR
                     "
BASE COUNT          980 a          881 c         1094 g          853 t
ORIGIN      
        1 gtggcgtggt atcacgctga acctgaggtg atatataatg gcaaaatcga tgtatgccta
       61 cgtgcgtgag gcatggaaaa aaccagctga aacaggggtc aaggaacttc agtggcagcg
      121 gatgcaggaa tggcggcgcg aggagagtgt cgtgcgactc gagcatccca ccagaatcga
      181 ccgggcgaga aaccttggtt acaaggcaaa acagggtatt gctgttgtcc gggtaaggat
      241 acgacgcgga gggcgccgca aaccccggta tgtgcgtgcg aggagatctg cacggatggg
      301 agtaaatcgg atgactgccg gaaaaagtct ccagcgtatt gccgaagaac gtgcatcggt
      361 caagtatcca aacatggaag tgctgaactc ctattgggta ggccaggatg ggaagcagaa
      421 atggttcgaa gtcattcttg ttgacggcaa ccacccctca ataaaagctg accgaaacct
      481 cgcctggttg aacaatcctg ttcaccgcgg gcgtgcggag cgcggtaaga cgagtgcagg
      541 gagaaagggc cgggggatga tgaaccgcgg aaaaggcact gaaaagacac ggcccagcat
      601 caggtcgcat ggcaatctgg gcaaataact tattctcttt tctctcacgt gtgttctgat
      661 gatggaaacg gatgcctgta tccatcctta tccggttgga actgcttcag ttcgcagaat
      721 ggcgatggaa gccggtgcgt tgggattcgg cagtattgta gtacctggtg aagtggctgg
      781 agtataccac ggggtgcgga tcattcctgc agtgatcatc agcgagacgg acctcaaaag
      841 ggttatcggg cagatcagga ggcacgcgaa tagcgggaac cttgtatttg tggatgctgg
      901 agataacggc tttaaccgtg ccgttcttgc ccagaaggga ttgaatgcca tcaggtgcct
      961 tcaccagata ccaaaaaagg catttgatca tatctctgcg cgtcttgcgt atgaaaatag
     1021 catagcggtc gagatcgatc tctacccgct tatccatgag aagggtattt tcagacagag
     1081 agtattgaac cgttacaatg acctgatgag attacattca cggtatcact tccctctgat
     1141 cctatccagc aatgcctgtt ctgttctcga tctccgttct cccgatgaga tccgtctcct
     1201 ctgttctctg tttggtatgg agaaggatga tacggatggg gcattgcaga ccatcagcac
     1261 cctcatatct ccagtcgaat cggtcatggt ggctccatga aaccccgccc ccctaccctg
     1321 agagaaaaga agcgatacct ccttcttcgt atcgacccgc caactccgga tcctgatcca
     1381 aaggatctat atttttcggt actggactcg gtgaccagtc tttatggaga cgcggaagcg
     1441 gccaggatga tgccggtcgt catttcctgt ggatcagggt atgcaatagt gagatgtatt
     1501 aggggttatg aagaaaaatt acggattgcg atcgtgaccc tgacctctgt tacggagcgg
     1561 aggattgcgc tgcacgcagt agccacttca ggaaccctgc tcgcgctcag gcgcagggtt
     1621 tcaggactat ctcctgtcag agtcgagacg atctcctctc tgcacttccg ggacagggag
     1681 tatacggcac ggcgttatca gggtcaaaag gttgatttgc aggaaaaagg aattaacagg
     1741 caggaactat tgtttctcac acaggatgat cttgagggat tataatgcag ccacaggcat
     1801 atcagatggg ttatgatcgg gcgataactg tttttagtcc ggatggccgc ctttatcagg
     1861 tcgaatacgc gcgtgaagcg gtgaaacggg gaacgactgc ggtcggaata aaatgtgagg
     1921 acgggatagt ccttattgta gacaagcggg tgagttcccg actgcttgag gcgtcttcca
     1981 tcgagaagat attcatgatc gatgaacaca ttggtgttgc ctcatcagga cttgtcgggg
     2041 atgcccgggc tcttgtcgac cgtgcgcgga ttgaatgcca gatcaatcgt gttacctacg
     2101 atgagaaaat ggatgttgaa gccctggcaa aaaaactctg cgatcatatg cagacctata
     2161 cccagtttgg tggagcacgg ccttatggca cggctctcct gattgccggg atcagtgatg
     2221 gcagatgccg gctctttgag acggatccat ccggcacgct tctggagtac aaggcgaccg
     2281 ggattggaat cggacgaaat gcagtgatga aggtgtttga ggaggaatat tcacatgagg
     2341 ctcctctgaa ggacagtatt ctcctggggt tgaaggcgct ccatgatgcg accgagggga
     2401 aattcgatgt gaataccgtt gaaatcggga ttattgagac aaagaccccc caattccgga
     2461 agatgaccaa ggaagaggta gcctccttcg ttgaacaatt tgaaaagtag gtgcaataat
     2521 gattcccctc gatagagcag tagtggcacg actcgaaagc cacggggaaa aattcgagtt
     2581 actggtagat cccgatcgtg cggtgaagtt cagacaggga gaaccggttg atctcgaaga
     2641 tatggtagcc gccctggcaa tctttgagaa ctcttcaaga ggaacacggg catccgaaga
     2701 ggctctgaat aaagtgttcc ataccaccga atttctccca gtcgctactc gaatcatcca
     2761 gaagggcgag atccatctta ctgccgaaca gcgacggcat atgattgcag agaagcggcg
     2821 tcaggtcatt acctttatcg ccaggaatgc agtaaatccg cagaccggtc tcccgcatcc
     2881 cccccagcgg attgaactgg caatggatga ggcacgggtg aatatcgatc tctacaaaag
     2941 tgttgatgaa caggtgaagg agacagtgaa agcgctccgc cctcttcttc caatccgatt
     3001 cgaagagatc cggatcgcgg tgaagatccc tcctgatttc gcatcgaaag cgttcgggga
     3061 gatccagcct gttgcccaga tagagaaaga tgagtggcag aaggatggat catggatctg
     3121 cgtagtcaga attcctgctg gcatccaggg tgatttttac gatctgatca atcggattac
     3181 caaggggcag ggcgaagtac ggatcctcaa tcaagtatat taacagcgag gacatagtat
     3241 aagagacata caagcgggtg actatatatg gccagcagga cacagaaagc aaaaggtaag
     3301 gtgtcaggga gcgccgggcg attcggtccg cgctatggaa ggtttgtcag gaaaagggtc
     3361 tctgacatgg aacaggtatc aaaggctgcc caccgctgcc cacgttgcga taccatcgcc
     3421 gtccgccgtc aggggaccgg catttgggga tgcagaaagt gcggtttcaa gtttgcaggt
     3481 ggggcttatc agccgcagac cccgcagctt cgcatcgcac taagaacaat cgaccgctcc
     3541 cttggcaagg aggcatgagc aggtttggct ggttcttata agtgcgcccg ttgcaagcat
     3601 aaggtcgaga ttgatgtcaa tgtccgctgc ccatattgcg gacacaggat cctttttaag
     3661 gaacgtggtg ctgcaatcaa agagttaaaa gctcgatgac cctcgtcagc acatcaagaa
     3721 aacctgtcgt ggaactccgt tcgcttgcaa aagactttgc cttcgctgcc ggatgccaat
     3781 atatcgtaag gggcaaggcg gggcttgc
//
LOCUS       DAOOCO010000176         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_22981_length_3797_cov_4.956708, whole genome shotgun sequence.
ACCESSION   DAOOCO010000176 DAOOCO010000000
VERSION     DAOOCO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22981_length_3797_cov_4.956708"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1551
                     /locus_tag="PK154_08970"
     CDS             94..1551
                     /locus_tag="PK154_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HPW11229.1"
                     /translation="MNTKKLIDLLKTWQVALLVILLIASVFLIYPHIEDDKFTTNLQY
                     GLDLSDGTWLQMEFQAEVVGFETERPAEEFLADLQKRLDAEIYLIDDDKLEIRKFFTR
                     DQLETAFAESGGRLTGYEQGVSRDTAEQVKKILEDKLNALGTKDAKVNPLTGLSGVSQ
                     YVRVELAGVSLNQAKEIVGKQGRFEIRVHTSGNATEHILYGDAINTVGVPTQQPPGGN
                     AWGVSFTLTEEGAAQFRDGAIKYGATVAPDQHYLDMLLDGNVVYSAPLSTDLAAQIHV
                     EPIRELYASTGSGEDGLNDAKTLEIHLRAGALPVEVKVAGSGYVPAALSDYFKTMAVI
                     VFIVAAIAVGIVIFFRYREPAIVLPMIGINMSEIVILLGIAALIQQQLDLAAIAGLIA
                     VLGTGIDQLVIITDEILHEGKVPSPNIYQKRLTRAIGIIMVSAATVIIAMLPLALMDL
                     STLRGFAIITILGVLIGVLVTRPAYGKIIMAILSK"
     gene            1591..1878
                     /gene="trxA"
                     /locus_tag="PK154_08975"
     CDS             1591..1878
                     /gene="trxA"
                     /locus_tag="PK154_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW11230.1"
                     /translation="MKVIQMGKPVVLDFFAEWCGPCRRQGPYLEDLKQNMGERIEIRK
                     IDVDQNMDLANKYGIRVVPTLIIEKDGKVVRTLEGVTSSDSLESMLLPLVD"
     gene            1879..2529
                     /gene="npdG"
                     /locus_tag="PK154_08980"
     CDS             1879..2529
                     /gene="npdG"
                     /locus_tag="PK154_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HPW11231.1"
                     /translation="MKIGIVGGTGDIGEGIAMRLSPNHNVVIGSREEEKAVESSDVCY
                     ATVTKLGLPCSVSGYSNQEAIDRSDIIILAIPFRHVEPTLASLHGFENKLVISPVNPM
                     EKRDFFVYTPPPDGSAALLIKRLLPKETRLCSAFNTVAANKWKALNETLDLAVPVCGD
                     DSLSKATVLDLVRSIPGVKAYDAGPLAVSSMVECLTPLLLNIARFNKMKDVGVQFR"
     gene            2545..3096
                     /locus_tag="PK154_08985"
     CDS             2545..3096
                     /locus_tag="PK154_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPW11232.1"
                     /translation="MRDAGEITEDFCRIGRRLVTEGLIKANFGNISIRSDDGFYITRH
                     GSYLDLPGELVYVPLVGQVPRKASSEYLVHRQIYNETPHRAVVHAHPPHAVALSLAEH
                     AISPVDSEGLLFCPFIPVVTGSPGSEELAQSVSHELLSSPVTIARGHGTFAGGKDLDE
                     AYIRTSLAEHSCRILILLKQIIP"
BASE COUNT          967 a          977 c          952 g          901 t
ORIGIN      
        1 caagtggtac atgcttggca ggggtggcaa tgtgaaggag acggaggata catcgaaagt
       61 aaaggtcagc cccgtcaaga ggaaaggatc gaaatgaaca ccaagaaact gatagatctc
      121 ctgaagacat ggcaggtcgc actccttgtc atccttctca tagcatccgt gtttctgata
      181 tacccccata tagaggatga caagttcaca accaatctcc aatacgggct cgatctctct
      241 gatggtacct ggcttcagat ggaattccag gctgaggtgg tgggttttga aacagagaga
      301 ccagccgaag aatttttagc tgacctccag aaaaggctgg acgcagagat atatctcatt
      361 gatgatgata aactggagat acggaaattc ttcacacgcg accagctcga gaccgctttt
      421 gcagaatccg gcggaagact gaccggttat gaacagggag tctccagaga tacggcagag
      481 caggtgaaga aaatcctgga ggacaagctc aatgcactcg gcaccaagga tgcaaaggtc
      541 aatcccctca ccggactttc aggggtctca caatatgtaa gagtagaact ggcaggagtg
      601 agtctaaacc aggcaaagga gattgtcgga aagcagggca ggttcgagat acgggttcat
      661 acttcaggca atgcaaccga acacatcctg tacggcgatg ctatcaatac tgtcggcgtt
      721 cccacacaac aacccccggg gggcaacgca tggggcgttt cctttaccct gactgaggaa
      781 ggtgctgccc agttcaggga tggagcgatc aagtatgggg caaccgttgc acctgatcaa
      841 cattatctcg atatgctgct cgatggaaat gtcgtatact ctgccccgct ctcaactgat
      901 ctcgcagccc agatacatgt agaacctatc cgtgaactct acgcatccac aggatcgggc
      961 gaagatggtc tcaatgatgc aaaaacactc gaaatccatc tcagggcggg agctttacca
     1021 gttgaggtaa aagtggcggg ttcgggttat gtgcctgctg cactttcaga ctatttcaag
     1081 acgatggccg tcatcgtttt catcgttgca gcgatcgctg ttggtattgt gatcttcttc
     1141 cggtaccgcg agcccgcaat tgtactcccc atgatcggaa tcaacatgtc cgagatagta
     1201 atcctcctcg gtatcgctgc gctcatccag caacaactgg atcttgcggc tatcgcaggg
     1261 ctgattgcgg tgctcggcac gggtattgac caactggtca tcatcactga cgagatcctc
     1321 catgaaggca aagtcccatc gcctaatatc taccagaagc gtctcacgag ggctattggt
     1381 attatcatgg tttcagcagc cacggtcatt atcgcaatgc tcccgcttgc gctgatggat
     1441 ctctcaaccc tccgtggatt tgccatcatc accatcctcg gggtccttat cggagtgctg
     1501 gtcacaaggc ctgcatatgg taagataatc atggcgatcc tctccaaata acccgataac
     1561 aatctttatc ttctttttcc accgaccact ctgaaggtaa tacagatggg aaaaccagta
     1621 gttctggact tttttgccga gtggtgcggc ccctgcagaa ggcagggacc ttatcttgaa
     1681 gacctcaagc agaatatggg agagagaatc gagataagga agattgatgt ggatcagaac
     1741 atggatctcg ccaacaagta tgggataagg gtcgttccaa cccttattat cgaaaaagat
     1801 gggaaagtgg tgaggacact ggaaggggtc accagttcag actccctcga atcaatgctt
     1861 cttccgctgg tggattaggt gaagataggt atcgttggtg gtacagggga cattggggaa
     1921 gggatcgcga tgcgcctctc cccgaaccat aacgtggtga taggatcccg ggaggaggaa
     1981 aaagcagtgg agtcaagtga tgtatgttat gcaacagtca caaaacttgg cctgccctgt
     2041 tccgtcagcg gttattccaa tcaggaggcc atagatcgat ctgatattat tatcctcgcg
     2101 atccccttca ggcatgtgga accaaccctc gcctcgctcc atggattcga aaacaagctt
     2161 gtgatatccc ctgtcaatcc catggaaaaa cgtgacttct tcgtctatac ccctccgcct
     2221 gatggttcag ctgcactcct tatcaaacga ctgctcccca aagagacgag actctgttct
     2281 gcatttaaca ctgttgcagc caacaagtgg aaagcactga atgaaaccct cgaccttgcc
     2341 gtcccggttt gtggcgacga ttccctttca aaggcgaccg tattggatct tgtcaggagt
     2401 attcctgggg tgaaagccta tgatgccgga ccactcgcag tctcctccat ggtcgagtgt
     2461 ttgaccccac tcctcctgaa cattgcacgg tttaacaaaa tgaaggatgt cggggtccag
     2521 ttcagataat ttttttctca tattatgagg gatgcaggag agatcacaga ggatttctgc
     2581 cggatcggac gcaggctcgt tactgaagga ctcatcaaag cgaattttgg gaacatcagc
     2641 atccgatcag atgatgggtt ctacataacc cggcatggat cgtaccttga tctccccgga
     2701 gaattggtgt atgttcccct tgtagggcaa gtcccccgca aggcttcaag cgaatacctc
     2761 gtccaccggc agatttacaa cgagacccca caccgagcgg tcgtccatgc ccatccgccc
     2821 catgcagtcg ccctttcact ggcggaacat gctatctcac cggtggattc ggaagggctg
     2881 ctgttctgtc ctttcatccc tgttgtaacg ggatcccccg gcagtgaaga gctggcacag
     2941 agtgtctcac acgaattgct ctcttcaccg gtaacgattg cccggggcca tggtactttt
     3001 gccgggggaa aagatcttga tgaggcgtat attcgcacct cactcgcaga acacagctgc
     3061 aggatcctca ttcttctcaa gcagataatt ccctgataaa gcgcagatga gtaataattc
     3121 ctggattcat cctgccacca gaagtcaggc ttttctgatc tgtggtggtt taataccgga
     3181 acagtgacag aaagaggtat acatatcata tcgaggttgc acatgggttc actcgaggaa
     3241 aaagtttccc gtctctcccc cgaacagcga agcgaggccg agatgttcat agactttctg
     3301 ctcatgaaat cggcatctgc ctccccaggt cctgcccaag atcctttccc gggttctgac
     3361 cattccattc ctgcttcccg acctatcatc atggcagaag agattcatgc acgccctgtt
     3421 acgacccgtc cgaatgatac tctcccttca ttaggtgacc tgaagccaca ggatacccat
     3481 ccaaaggatg agcgtgtgtc tcagccctcc cgatccaaac ccaaagaccc ggggctgttg
     3541 ctggactgga tcgattaaac gaaggaaact cccgctactg gctggatggc ctccgtccac
     3601 ctcttctcca tcgggttcaa ctgaactttc gggtttcctt cggatagaag aaaatcacat
     3661 gattccccgt tgagacagct gcttatagat taagaggcgc aagctgaatt tgtattggaa
     3721 aggagactga gtcattggaa aaagatggac tcgacgggat tcgaacccgt ggccttcacg
     3781 ttgcaaacgt gacgatc
//
LOCUS       DAOOCO010000177         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_23210_length_3773_cov_4.246638, whole genome shotgun sequence.
ACCESSION   DAOOCO010000177 DAOOCO010000000
VERSION     DAOOCO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23210_length_3773_cov_4.246638"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK154_08990"
     CDS             complement(<1..205)
                     /locus_tag="PK154_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW11233.1"
                     /translation="MPILLALILMVGMGSAATTEVHIVRLADDGITIVNETTVTYQWM
                     EAKLPVFGDGNTYYYYQGPIFEGE"
     gene            complement(519..3761)
                     /locus_tag="PK154_08995"
     CDS             complement(519..3761)
                     /locus_tag="PK154_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW11234.1"
                     /translation="MKYLCTLLVILCLLPAIAVPVGGATTAVRVVLYAADGYTILNET
                     TVDYRWMESHLPVMGDGTTQYYHQGPVFVDDPDEATEEALRWNPEEDVNVESKAMGAV
                     KGTDVRDLCDLVGGMQPDEEVKIVSADGWSMRFAYTNVYEYSGREGPMILAWYNGAEA
                     PGTWERQGTGYVPDYQSGMRLIWLADTSTNPQGLHAFGNYDWHEAAEPEYWYYYQSGG
                     ERYPTTTGLSGKYISEIHILSNREPEGSIEITSNPPGARVSLDGVDSGFETPCNIDSL
                     LEGVYSLTVSKGGYLTPEERLVEVLPGKSLPVHFDLVPAPAGGGDSGTDSSGSGPVSP
                     LPDLAILEGGQLAVTEFLRLNGSFSAHPSNTLPFTLKGGEEQAITFDGISSGSSPGLL
                     RFYLFVDQSSADPGIDADPEIVLVTDEGEEGPARTWVERGEDDQPYATTLVFSLPVLD
                     PDGTMIVRSRNHASWNSTVAGGLLLVGNEDPGAGTTGVWIAEGADLIGNAPGLGSPMT
                     LAEFAGPFPGQGPDGTLIVATTPGSSAGNLSFFINGASTPGRLVFSESPVAVHEILVR
                     EGAGSMVRLHTGAGDSVVTNRVAILAMDIPDQKGDTAGTIPITGKITLNTTTPAVISP
                     GVSIPAREGSTPPTSPPGSPMLPASDPIGRFLCWLHNFVLWLQGMPPEPCYQRTITVP
                     PVSDSILPPEEIPAEQNFTIHVTSTPHGASVSLDGQPTGLITPCDLMVPAGGVHTIRL
                     EKDGYQPAEQVIQGNGSIELLLHPLMPAVVITTLPAGAPALASGKSHHGGIYIQSYPE
                     QAEIKIDGVIVGTRSPLLVSPLREGFHTVSAGILTTGNAYASQETIRTWVFPDAILPV
                     KFNLMDAASPGSVTIGGEYWKGTSFTVNGYYPVKRVPEKVELAEHPSFVTLMKDAAYY
                     SYTIPQSSRDSGEFLIPAKTPMVCNLSIGSDPAGAEIFIDGIRTGLLTPAIIPNVSEG
                     YHRVSLTAENRIPVTERIFIAESQCLLGEYQVQYSLPWYASGSIGLTSDPPGAAVSIR
                     GLRTGEVTPCTIEGIPIGVWEVLLTLEKAKRYLDVTVEPGQTRNYSVVFD"
BASE COUNT          820 a         1072 c         1035 g          846 t
ORIGIN      
        1 attccccctc gaagattgga ccctggtagt agtagtaggt atttccgtca ccaaataccg
       61 gaagttttgc ctccatccac tgataggtga ccgtagtctc gtttactata gtaatcccat
      121 cgtccgcaag tcgtacaata tgtacttcag tagttgcagc cgatcccatt cccaccatca
      181 gaatgagggc aagaagaatc ggcatatatc gtccttgcaa tcttattgac atctctctac
      241 caccttgatg agggagaaac tcttcggaca tctcttcgct tgtttccggt atttgaagcg
      301 gcgaaaatga tgaggaagtg aatgtatagg aatgggtttt cgtttaatta catatataaa
      361 tttttgtaaa taataacatg ttctgcataa ccaactggtt gtatggtccg ggttgggatt
      421 tttagtaagg atgctgtgtg agcgaggtcc cgtaggcttc ggttgtcggg tgatatttgc
      481 tataccccgt tagcggtatt tgtatcaagc actcaatctc aatcaaacac caccgaatag
      541 ttcctggtct ggcctggctc aacggtaaca tcaaggtatc gcttcgcctt ttcaagggtg
      601 agcagaacct cccagacccc tatcgggatt ccctcaatag tacagggtgt cacctctccc
      661 gtccggagac ccctgatgga gaccgccgcc cccggcggat cactggtgag accgatgctc
      721 cctgaagcat accacggaag tgaatactgt acctggtatt ctccgaggag gcactggctt
      781 tcagcgatga aaatacgctc ggtcaccggt atcctgttct ctgcggtgag cgaaacccgg
      841 tggtacccct cagatacatt cgggatgatg gcaggagtaa ggagcccagt gcgtattcca
      901 tcgataaaaa tttcagcacc ggctggatcg ctcccaatcg agagattgca gaccatgggt
      961 gttttggccg ggataaggaa ctccccactg tcccggctgc tttgagggat cgtataggaa
     1021 taataggcgg cgtccttcat gagagtgaca aacgacggat gctctgcgag ctcgaccttt
     1081 tcagggaccc tctttaccgg gtaataccca ttcaccgtga acgatgtgcc tttccagtat
     1141 tccccgccga tggtgacaga acccggcgag gcggcatcca tcaggttgaa cttgacgggc
     1201 aagatggcat ccgggaagac ccatgtccgg atcgtctcct gcgatgcata ggcgttcccg
     1261 gtcgtgagta tccctgccga caccgtgtgg aatccctccc tgagaggaga gacaaggaga
     1321 ggagacctcg tgcccacgat caccccgtcg atcttgatct cagcctgctc tggataggac
     1381 tggatatata tccccccgtg atgtgacttt ccggatgcca aggcgggggc tcctgcaggc
     1441 agcgttgtta tcaccactgc cggcatgagg ggatggagga gcagttcgat ggatccattg
     1501 ccttgtatga cctgttctgc aggctgatat ccgtctttct ccagtcgtat cgtgtggact
     1561 cctccagcag gcaccatgag atcgcacggg gttatcagtc cggtaggctg gccatcaaga
     1621 gagacgcttg cgccatgcgg ggtagaagta acgtggatgg tgaaattttg ctctgcaggg
     1681 atttcctccg gaggaaggat actatcgctt acaggaggta ccgtgatggt ccgctggtag
     1741 cagggttcag gaggcatgcc ctgcagccag agaacgaaat tatggagcca gcagaggaat
     1801 ctcccaatcg ggtcgctggc aggcagcatc ggtgaacctg gaggtgacgt gggtggagtt
     1861 gacccctccc tggcaggtat cgataccccg ggggagatga cagcaggggt ggtcgtattg
     1921 agcgtgatct tccccgttat cggaatggtc ccggccgtat ctcccttctg gtcagggata
     1981 tccatggcaa ggatcgctac gcggtttgtc actacggagt caccggcacc agtatgtaac
     2041 cgcaccatgg acccggcccc ctcacgaacc aggatctcgt ggacggcaac cgggctctcg
     2101 ctaaaaacca atcgacccgg cgtactggca ccgttgatga aaaagctcag attgcctgcc
     2161 gatgagccag gggtggttgc cacgatgagg gtgccatctg gtccctggcc agggaacggc
     2221 ccggcgaatt cagccagggt catagggctc ccaagtccgg gtgcattccc gatcaggtcc
     2281 gccccttctg caatccatac cccggtagta cctgctcccg gatcctcgtt tcccaccagc
     2341 agcagacctc ctgctacagt agaattccag gaagcatggt tcctgctccg cacgatcatc
     2401 gtcccatcag ggtccagtac cgggagagaa aataccagcg tcgttgcata aggctggtcg
     2461 tcctcgcccc gttcaaccca ggtcctggcc gggccctcct ccccctcatc ggttaccagg
     2521 acgatctctg gatcagcatc gatcccggga tcggcgctgc tttgatcgac gaacaggtag
     2581 aaccggagaa gcccgggaga ggagcccgag ctgatcccgt caaaggtgat ggcctgctcc
     2641 tccccgcctt tgagggtgaa cggcagggta ttggagggat gggcggaaaa gctgccgttc
     2701 aagcggagaa attccgtcac tgccaactgc cctccttcga ggattgccaa gtccgggaga
     2761 ggtgagaccg gaccactccc cgatgagtcc gtgccagaat ctcctccccc ggcaggggca
     2821 gggacaagat caaaatgcac aggcaggctc tttccaggta acacttccac caatcgctct
     2881 tcaggggtca gatagcctcc cttgctgacc gtgagggaat acaccccctc caagagcgaa
     2941 tcgatattgc agggggtctc aaatccgctg tcaacaccat caagggagac ccttgcccca
     3001 ggaggatttg aggttatctc gatcgaacct tccggttccc ggtttgagag gatatggatc
     3061 tctgagatgt acttcccaga gagccctgtc gtggtaggat acctctcacc ccccgattga
     3121 taataatacc agtattccgg ctctgccgcc tcgtgccagt cgtaatttcc gaaagcgtgg
     3181 agcccctgtg gattggtgct ggtatcggcc agccagatga gccgcatgcc tgactggtaa
     3241 tccggcacat acccggtgcc ctgccgctcc caggtccctg gcgcttctgc cccgttatac
     3301 caggcaagaa tcatgggccc ttcccggcct gaatactcat atacattggt ataggcaaac
     3361 cgcatgctcc acccgtcggc tgaaacgatc ttcacctctt cgtcaggctg catccctccg
     3421 acaagatcgc agagatccct gacatccgta cccttcaccg cacccatcgc ttttgattcg
     3481 acattcacat cttcttccgg gttccagcgg agcgcctctt cggtcgcttc atcaggatca
     3541 tcgacaaaga ccggtccctg gtggtaatac tgggtcgtcc cgtcacccat caccgggagg
     3601 tgtgattcca tccagcggta gtcgaccgtg gtctcgttga ggatggtgta tccatcggca
     3661 gcatagagaa caacccgtac tgcggtggta gcccctccaa ccgggacggc tatggccggg
     3721 aggaggcaga ggatgacgag cagcgtgcag agatacttca cggtacattc ccc
//
LOCUS       DAOOCO010000178         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_23484_length_3743_cov_5.002983, whole genome shotgun sequence.
ACCESSION   DAOOCO010000178 DAOOCO010000000
VERSION     DAOOCO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23484_length_3743_cov_5.002983"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK154_09000"
     CDS             <1..969
                     /locus_tag="PK154_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HPW11235.1"
                     /translation="KKKRSITNYIVLLTLATIIATYGVISGSTATVIGAMIIAPLMTP
                     IMAATLAMVLGRGTRIKRSILVVSFSVLYVIGLALLLSLFISPIVIGFGSNPEITTRV
                     SPNLLALFVALASGAAGAFAISREDVGDTLPGVAIAISLVPPLSVVGISLSKLHWGDA
                     SGAFVLFLTNFLAIILAGGAVLWLSGVSVSRITPQRDANRKRAFELAILATIIVAVIL
                     GFNGYRTLEQERDTALANTAVTSWLEGTSYTVTRLTLNFQPRDILLNGPAHVTISIAG
                     TGELESIDELAKNMEDALGYPVTIQLDVLPTQVNYYPRLLRQAASG"
     gene            complement(986..1699)
                     /locus_tag="PK154_09005"
     CDS             complement(986..1699)
                     /locus_tag="PK154_09005"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPW11236.1"
                     /translation="MAEILRQSMSTRDLGWLSSREETPKRSMYLRLLIPPLIAVLFFL
                     VCLLILPPERILILGGLMLAYYVPPAGKESIIPIGIGLGIPWWLMAITVILLDVITSL
                     FMIMNFVLMLRVPRLGPWIEKTTLTGREFMEEHPWMARWRIPGVAFFVMLPLQGTGGV
                     GATVVGLMAGLSPGEILLAVGIGAAIESLIFALGSELIWRLLMAHFIAGTVVAVGLIL
                     CVILIFVYFNRHMAGKKKL"
     gene            complement(1771..1941)
                     /locus_tag="PK154_09010"
     CDS             complement(1771..1941)
                     /locus_tag="PK154_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11237.1"
                     /translation="MIKLKILLNKLTPGDTLTFFATREQVDNTCAPFSSNGYQVAWDQ
                     EAENRYLVRIGK"
     gene            complement(2011..3192)
                     /locus_tag="PK154_09015"
     CDS             complement(2011..3192)
                     /locus_tag="PK154_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPW11238.1"
                     /translation="MEHSSIQSSTFFFSTPLSHERINWYTECIASGAGIEPGQKRAFT
                     VFITGDALFSFVDAQSREAWNALSEVPSVQIVADGDELRLHGLGDSVGAASPGITITE
                     RGAGTLFWGQLVSHLSSEWKGTKKAAFLLCHPPYMSRIPVHMLRFLSGAHAAGLSPEL
                     YAYLDGLHNIHNGQRPSEFENIGRAIATLARKSGESGKDPWFAACSRCATARGYFQMN
                     PGTGYCEPASSIDEMTIRPLKEILSRFSGYYPILSHTSGEIVKHAETSDQDTRPRLII
                     FITNPPYGTEWTFGGLSLALATAMEGIPTTVVFIEHGVYSLYGTHEVPPNDKVFNVQE
                     MVAATLDIPSLCYLVHAPSLERRGIGVEEHLTGIREVQNDDLAQILWGQDNSATRILF
                     F"
     gene            complement(3197..3721)
                     /locus_tag="PK154_09020"
     CDS             complement(3197..3721)
                     /locus_tag="PK154_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPW11239.1"
                     /translation="MATGILSKEVAIYAVPLVTLLLGILIGYLAQRSGYCSIGGFRDY
                     VLFRHTRLLSGYVTLIVASFFGYLLFWFLTPAAMEHFFWALTSNPLMPVPGAPAGLSP
                     AAYLILAAIPGFLIGLICVLLGGCPIRQTVMASEGSYKAAFFFIGMCVGSVIFAAFIS
                     GWVVVLMRAIGLGA"
BASE COUNT          948 a          996 c          953 g          846 t
ORIGIN      
        1 aagaagaaac gctcgataac aaactatatt gtccttctga cactggcaac aatcatcgca
       61 acctacgggg taatatcagg atcgacggca acggtcatcg gggcaatgat cattgcaccc
      121 ctgatgactc ccattatggc ggcaaccctt gctatggtac ttggcagggg gactcgcatc
      181 aaaagatcga tcctggttgt ctcgttcagt gtcctctatg tgatcgggct ggccttgctt
      241 ctctctctct ttatctcacc aatcgttatt ggatttggct caaatccaga aattaccaca
      301 agagtctccc ccaacctgct ggctctcttc gttgcccttg cttcaggagc agccggtgcc
      361 tttgcaatct cacgggagga tgtcggggat accctccctg gagtggcaat agctatctca
      421 ctcgtacctc ccctgtcagt ggttggtatc tctctttcca agcttcactg gggagatgcc
      481 agcggggcct ttgttctttt cctcaccaac ttcctcgcaa tcatcctggc aggaggagct
      541 gtcctgtggc tctcaggagt gagcgtcagc aggattacac cgcaaaggga tgcgaaccgc
      601 aaacgtgcgt tcgaattagc catcctcgct accattatcg ttgcagtcat ccttgggttc
      661 aacggatacc ggactcttga acaggagaga gataccgcac tcgcaaacac cgcggtcaca
      721 tcctggctgg aggggacttc gtataccgtc accagactga ccctgaattt tcaaccccgc
      781 gacatcctgc tgaatggacc ggcacatgtc acgatttcta ttgctggtac tggagaactc
      841 gaaagtatcg atgaacttgc aaaaaatatg gaagatgctc ttggttaccc ggtcaccatc
      901 caattagatg ttcttccaac ccaggtcaat tattatccga gacttctgag gcaggctgct
      961 tccggataat gtccgggaac cccggctaca actttttttt tcccgccatg tgccggttga
     1021 aatagacaaa gatgaggatt acacaaagaa tcaatccgac agctacaaca gtccccgcaa
     1081 taaaatgtgc catcaggagc ctccagatca gctcagaccc aagggcaaat atgaggctct
     1141 cgatcgctgc tccgattcct accgcaagaa ggatctcacc aggtgataat ccagccatca
     1201 atcccacaac cgtggcgccg acaccgccgg ttccctggag cgggagcatg acaaaaaatg
     1261 caaccccggg gattcgccac cgggccatcc acggatgctc ctccatgaat tccctccccg
     1321 taagggtagt cttctcaatc catggtccga gccgcggcac cctgagcata agaacgaaat
     1381 tcatgatcat gaagagcgag gtgatgacat caagaaggat cacggtaatc gccatgagcc
     1441 accagggtat cccaagtccg atcccgatcg ggatgatcga ttccttccct gccggaggga
     1501 cataataggc cagcatgagt cccccgagaa tcaggatcct ttcggggggg agaatgagga
     1561 ggcagaccag aaagaagaga accgcaatga gaggagggat gagaaggcga aggtacatgg
     1621 aacgtttcgg ggtctcctcc ctgctggata accacccaag atcacgggta ctcatgctct
     1681 gccggagtat ttcggccatt ccctcatctc tctttcttta ccacatagca gagtcggggg
     1741 gagaagagat cctgacgatg taggtttcta ttattttcca atcctgacca ggtagcggtt
     1801 ctctgcttcc tggtcccatg caacctgata cccgttactg gagaaggggg cgcaggtatt
     1861 atccacctgc tcccttgttg caaaaaaggt aagggtgtct cccggagtaa gcttgttcaa
     1921 caggattttt agcttgatca ttagatttgt acaagttaga tcagtaaaat cattcaccct
     1981 tccatcaccc tttattgtaa tactcttggc tcagaaaaat aatatccttg ttgcagagtt
     2041 gtcctgtccc cagaggattt gcgccaggtc atcattctgt acctcccgaa tccctgtgag
     2101 gtgctcttca actccaatgc ctctccgttc cagggaaggt gcatgaacaa gatagcagag
     2161 cgaagggata tccagggttg cggcaaccat ctcctggaca ttgaaaactt tgtcgtttgg
     2221 agggacctca tgcgtaccgt agagggaata cacgccgtgc tcaataaaga cgacagttgt
     2281 gggtatcccc tccatcgctg tcgcaagggc gagggagaga cccccgaagg tccattcggt
     2341 gccatagggg gggtttgtga tgaaaataat gagccttggc cgggtatcct ggtcactggt
     2401 ttcagcatgt ttgacaatct ccccgctcgt gtgtgaaagg atgggataat atccggaaaa
     2461 ccgggagagg atctctttta agggtcgaat cgtcatctca tcaatgctcg aggctggttc
     2521 acaatacccg gtccccggat tcatctggaa atatcccctg gctgttgcac agcgggaaca
     2581 ggctgcaaac cagggatcct ttcccgattc tcccgatttc ctggcgaggg tagctatagc
     2641 ccggccgata ttctcaaatt ctgacgggcg ctggccgtta tggatattgt gtaatccatc
     2701 aagataggca taaagttcag gagagagacc tgcagcgtgg gctccagaga ggaaccggag
     2761 catgtgaacg ggtatccgac tcatataggg aggatggcag agcagaaatg ccgccttttt
     2821 tgtgcctttc cactccgaag aaaggtgtga taccagctgt ccccagaaca gtgttccggc
     2881 tccccgttcc gtaatggtga tgcccgggga tgctgcgcca actgagtcac cgagcccgtg
     2941 gagccgcagc tcatctccat cggcaacaat ctgtacggaa gggacttcag agagagcatt
     3001 ccatgcttca cggctctgtg catcaacaaa actgaacagt gcatctccgg ttataaacac
     3061 cgtgaaagct cttttttgtc ctggttcaat tccggcacca gatgcaatgc attccgtgta
     3121 ccagttgata cgctcatggg agaggggcgt gctgaaaaag aatgttgagg actggattga
     3181 tgagtgctcc atatcttcat gcaccaagcc cgatcgcccg cataagaacg accacccatc
     3241 ccgagatgaa ggcagcaaag atcactgaac caacgcacat gccgataaag aaaaatgcgg
     3301 ccttgtagct cccttctgat gccattactg tctggcggat agggcatccg ccaaggagaa
     3361 cacaaatcag tccgataaga aatccaggaa ttgcagccag aatcagatat gcagcaggag
     3421 agagcccggc aggagctccc ggaaccggca taagcggatt gctggtaaga gcccagaaga
     3481 aatgctccat agccgcgggc gtcaggaacc agaagaggag atacccaaaa aatgacgcga
     3541 cgatgagagt gacataccct gataaaagcc gggtatgtcg gaagaggaca taatcccgga
     3601 atcctccaat tgaacagtac cctgaccgct gcgcaaggta cccgataaga atgccgagga
     3661 gcagcgtgac gagggggacc gcatagatgg ccacctcttt tgaaagaatg ccggtcgcca
     3721 tcagttcatc tccttttcag cct
//
LOCUS       DAOOCO010000179         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_23485_length_3743_cov_4.593818, whole genome shotgun sequence.
ACCESSION   DAOOCO010000179 DAOOCO010000000
VERSION     DAOOCO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23485_length_3743_cov_4.593818"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK154_09025"
     CDS             <1..212
                     /locus_tag="PK154_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HPW11240.1"
                     /translation="RGHEFHYSDVNLPADTRFSYLLSRGKGIKGQLDGAVRDNTIGSY
                     THLHPVSSRNMFSSFIASCRTGARG"
     gene            254..1123
                     /gene="ilvE"
                     /locus_tag="PK154_09030"
     CDS             254..1123
                     /gene="ilvE"
                     /locus_tag="PK154_09030"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HPW11241.1"
                     /translation="MIIYIDGKYLPKEEAHISVFDHGFLYGDGVFEGIRAYNGRVFRL
                     QEHLDRLYDSARTIDLKVPVPKEEMAGIILEVLRRNKLSNGYIRPLVTRGVGDLGLDP
                     RKCPIASVIVIAVEWGAMYGDLYEKGLKAITVSVRRNPAEALPPNVKSLNYLNNILGK
                     IEANYKGGDEAIFFDTNGYMSEGSGDNIFIVKNGIIFTPPTLNNLRGITRAVVLEIAK
                     SHGLTLVEQNLGYYDMYSADEVFVTGTAAEVAPIVLIDGRAIGTGKPGPVTRQLMAAF
                     RTVTETEGTPINQ"
     gene            1189..1263
                     /locus_tag="PK154_09035"
     tRNA            1189..1263
                     /locus_tag="PK154_09035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1225..1227,aa:Glu,seq:ttc)
     gene            1798..2622
                     /locus_tag="PK154_09040"
     CDS             1798..2622
                     /locus_tag="PK154_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW11242.1"
                     /translation="MVAAEKPGIEFPSVLDRAYKEYKRHLKVEDIMSREVAITTPDTI
                     MADAAKQMGEKHIGSLIVMQYGTPIGIVTERDLLSKILAGGLDIGTTKVSHVMSYPLV
                     KICPDLEIREAARTMIRRKGRLAVFQCGDIAGVITASDLIREMPDAPETSLTVDEFMT
                     RDLKSIGEDETVEEAARLMGRERIGSLLVMRDDHPLGIFTERDLLTTFIARNRPLDAP
                     VSMASSSPLITAPAGISIHAAAKTMASRHIRRLILMKKKTLEGIITARDLVEAYAR"
     gene            complement(2816..3712)
                     /locus_tag="PK154_09045"
     CDS             complement(2816..3712)
                     /locus_tag="PK154_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPW11243.1"
                     /translation="MHQVMRGYVDLLRLHFFFAWPVLFCSGYLVATSVYGLFSWYDLL
                     KVAFIGFLGFEAGFVLNDYVDRHYDTRDIETGKLTKYWRMFGKRPIPEGLVSSREAVV
                     VFIVLVTATVLLTATLPFPHSAFVLLIMLCSYGVEVFYQVRKRNQRFPLAQLVGRTDF
                     ALFPVAGYLCIGSPDMLALFYFMFFYPFAQAHLGANDLIDVANDRARGMNTVATLYGL
                     SGTALWIAGFTAIHVVTAVLFMTRLGWIARGGIVLGLCLLLVANMAISRDRTPDTGLR
                     MLPLFHITMLIYAVSIGLDSFF"
BASE COUNT          960 a          978 c         1016 g          789 t
ORIGIN      
        1 tcaggggcca tgagttccat tactctgatg tgaatctccc ggcggatacc cggttctcct
       61 accttctctc gcgtggcaaa gggataaagg ggcagctcga tggtgccgtc cgggacaata
      121 caatcgggag ctacacccac cttcacccgg taagcagccg gaacatgttc tcgtctttta
      181 tcgcctcctg caggaccggc gcccggggat aaccagcagg taaaaaatac tagtacttcc
      241 aaatctagca ggtatgatca tctatattga cgggaagtat ctgccaaagg aagaggcaca
      301 catctctgtc tttgaccatg gattcctcta tggggacggg gtctttgaag ggatccgtgc
      361 ctacaacggc agggtcttcc gcctccagga gcacctcgac cggttatatg actctgcacg
      421 gaccattgac ctcaaggtcc cggtcccaaa agaggagatg gccgggatca tccttgaggt
      481 gctcaggagg aacaagctct cgaacggcta tatccggcca ctcgtcacga ggggtgtggg
      541 agaccttgga ctcgacccga ggaagtgccc gatagcctcg gtgattgtga tcgccgttga
      601 atggggtgcg atgtacggtg atctctatga gaagggcctc aaggcgatca cagtctcggt
      661 caggaggaac cctgccgaag cactcccgcc gaatgtcaag agcctgaact acttgaacaa
      721 catcctcggc aagatcgagg cgaactacaa gggcggcgac gaggcgatat tctttgatac
      781 caacgggtat atgtcggagg ggtcgggcga caatatcttc attgtgaaaa acgggatcat
      841 cttcaccccg ccaacgctga acaacctcag ggggatcacc cgtgccgtag tccttgagat
      901 tgcgaaatca cacggcctta ccctggtgga gcagaacctc gggtattacg atatgtattc
      961 agcagacgag gtctttgtca cgggcactgc ggctgaggtc gccccgattg tcctgatcga
     1021 tggccgtgcg atcggcaccg gaaaacccgg ccctgtcacc cgccagctga tggcagcgtt
     1081 caggacggtc accgagactg aagggacccc gatcaaccag tgatactcct cgccatcctc
     1141 atccacaatt atttttacgt tgaagatcta aaaggataaa gcaactttgc tgctatagtg
     1201 tagtccggcc aatcatttcg gcctttcacg ccgaagactg gggttcgaat ccccatagca
     1261 gcactctttt catgcccccc gatttatgcg attagcaacc agatgttccc gatagcctcg
     1321 agggatgagg ggaatgatgg cgctgaatgc gggatcactt ctggtacctg gatcacctta
     1381 aaattaagcc cattttgggc tccgtttccc ccattttgca catgggaccg catttcgatt
     1441 ggaaattgtg tttatacaca tggataatat tgacaaaatc gcttcatttc aaaaaatagt
     1501 cgcaaatatc tgattaaaaa tgatctcttt attgaggttc tgtacgagag aaataggagg
     1561 tagtagaata ctcggatgga accggaagaa gcccgtgcat gcaatctgtg aaggactttt
     1621 gcggaccagt aatcggttga ttttatggga ttaaaagaat atttaaacgt ttattttcaa
     1681 tatatcgcgt ttacagggct ttttagtggt tttaggagag gatggcatgc cagatgagcg
     1741 gttatacctc cgaaaccttt aaacatctgg agctgatcct cacaatgggg actttcaatg
     1801 gttgcagcag aaaagcctgg tattgaattc ccgagcgtgc tcgaccgtgc ctacaaggaa
     1861 tacaagcgcc acctgaaggt ggaggatatc atgagtaggg aggttgccat cacaaccccc
     1921 gacaccatca tggcagatgc cgcaaagcag atgggtgaga aacatatcgg gagcctgatc
     1981 gtcatgcaat atggcacccc gatcgggatc gtgaccgaac gggacctgct ttcaaagatc
     2041 ctggcagggg gtcttgacat tggaacgaca aaggtcagcc atgtgatgtc gtacccgctc
     2101 gtcaagatct gtcccgacct ggagatcagg gaagcggcac ggaccatgat ccggcgaaaa
     2161 gggaggctgg ccgtcttcca gtgcggggat attgcagggg tgatcacggc atcagacctc
     2221 atccgggaga tgcccgatgc tcccgagacg tcccttaccg ttgatgaatt catgacgcgg
     2281 gacctaaaaa gcatcgggga ggatgagacc gttgaagagg cagcccgtct catgggcagg
     2341 gagcggatcg gcagcctgct cgtcatgagg gatgaccacc ctttggggat attcacggaa
     2401 cgtgatctcc tcaccacctt catcgcccgg aacaggcccc ttgatgcccc ggtgagcatg
     2461 gcatcctcat cgccgctgat cactgctccc gccgggatca gcatccatgc cgccgcaaag
     2521 acaatggcat caagacacat ccgcaggcta atcctcatga agaagaagac cctggaaggg
     2581 atcatcaccg cgagggacct tgtagaggca tacgcccggt agcctggcat cacctgagaa
     2641 cagaagggta tcagggaggg ctagtccacc ctttgagatc cttccttcct gtcactccaa
     2701 aggaatgggt acgatccgct ttgacgggac ttgacccgtg ttcgaaaaga agagggatat
     2761 agtgtttctc cgaaccgcat tcccgggcag cataccctga attttctctt tccgttcaga
     2821 agaagctgtc aagaccgatc gacacggcat aaatcagcat agtgatgtgg aagagcggaa
     2881 gcatccgaag gccggtatcc ggggtcctgt ccctgctgat cgccatgttt gccacgagaa
     2941 gcaggcagag cccaagaacg attccccccc ttgctatcca gcccagacgg gtcatgaaga
     3001 ggactgcggt gaccacatgg attgcagtga agcctgcaat ccagagcgct gttcccgaaa
     3061 gcccgtacag ggttgccacc gtattcatcc cccgtgccct gtcatttgcg acatcgatca
     3121 ggtcatttgc tccaaggtgg gcctgggcaa acgggtaaaa gaacatgaag taaaagaggg
     3181 ccagcatatc gggagagccg atgcacaggt acccagcgac ggggaacagg gcaaaatcgg
     3241 tgcgtcccac cagctgtgca agagggaaac gctggtttcg ctttctcacc tggtaaaaga
     3301 cctcgacacc ataactgcag agcatgatca gcaagacgaa tgccgaatga ggaaaaggaa
     3361 gggtcgcggt gaggaggacg gtcgctgtca caaggacaat aaatacgaca actgcctccc
     3421 gggaagagac cagcccctcc ggaatcggtc gcttcccaaa catcctccag tacttggtca
     3481 gctttccggt ttcgatgtcc cgggtatcgt aatgcctgtc aacatagtca ttgaggacaa
     3541 accctgcttc aaatccaagg aaaccgatga acgctacttt caggaggtca taccacgaga
     3601 agagaccgta tacggaagtg gcaacgaggt atcccgagca gaagaggacc ggccatgcaa
     3661 aaaagaaatg gagccgcagg agatccacat aacctctcat gacctgatgc atacagggat
     3721 tttcaacacc atcataattc agg
//
LOCUS       DAOOCO010000180         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_23830_length_3703_cov_5.535362, whole genome shotgun sequence.
ACCESSION   DAOOCO010000180 DAOOCO010000000
VERSION     DAOOCO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23830_length_3703_cov_5.535362"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PK154_09050"
     CDS             <1..916
                     /locus_tag="PK154_09050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW11244.1"
                     /translation="PMIDVFNSLVESQKLPIFSIAGEPYNELLARIGTQAEVDIIILG
                     GMGLKYDDYLFFRDTLEEHGALSHSIVFMHTAADPVVECLLVPDISLAVAENFALEGK
                     RVLVLLTDMSNFCDALKEIAITMQQIPSNRGYPGDLYSLLASRYEKAVDFETAGSITI
                     LAVTTMPGDDVTHPIPDNTGYITEGQFYLKNGAIEPFGSLSRLKQLVNAKTRDDHRTI
                     MDTMIQLFADYRETQEKQSMGFQMSAWDMKLLKYGRLFEEKMMSLKVNIPLEKALDLG
                     WEILHDCFLPEETGIKRSIIDQYWPKKP"
     gene            913..1524
                     /locus_tag="PK154_09055"
     CDS             913..1524
                     /locus_tag="PK154_09055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW11245.1"
                     /translation="MKIKFTQGELKKQRDALRQYERYLPTLQLKKQQIQLEILQQQSS
                     LRGREKTLSEKIRSAEEWSALLPEVPGIRMWLKPERIVTGKRNIAGVDIPIFENVYFE
                     PAEYNLFLMPLWLDTGLDALKELTSLQEEILVIQRGISVLNQELRITTQRVNLFEKVK
                     IPEAKEAIRLIKIYIGDQMTNAVGRSKIAKNKIEQSAFEEIAL"
     gene            1533..3287
                     /locus_tag="PK154_09060"
     CDS             1533..3287
                     /locus_tag="PK154_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11246.1"
                     /translation="MKKATIIFQSGNADATVDTVRKLGVMQVEHQNIPEGGKISALLE
                     KVALINSSIDVLNQLPISERKNQPQGPISGDWREVAGSIIETGRKQEQLELSRRNIIG
                     QINEWERWGDIDQAEVRSLAQNGIHLKLYQVPVRELGKFPDDIVVKTIYIAGDIAHVV
                     AISRREFECGFKEVIPPEQGLSRLKERLAEHTRSMEMNKSGILNSAGFYDDLLDIKRR
                     LEKEIEFQEVLAGMGTEGALSYITGYVPSDLEDSLIGEARKNGWGILIAEPSPDDNVP
                     TLLRNPRWVEMIKPVLGLLGLTPGYRELDISLVFLIFFSIFFGILIGDAGYGLVYVII
                     TLLLSLWLKKKMMMTGEMKITLILFYLLGSCAIIWGVLTGTFFGQWGLTALVPELNDS
                     AFVTTLCFFLGALHLSIAHCWRAALKYPSVKALADVGYICILWTAFFLANTLILGDPF
                     PSFGLWLVIAGIILVILFTNPKRSNIFKGIGEGLGTIALSFMNNITDVISYIRLFAVG
                     MATLAVANTTNYLASGFGSGIIAGVAGAVILLSGHSLNLILGLMSVLVHGVRLNVLEF
                     SGHANVTWSGTPFEPLKE"
     gene            3301..>3703
                     /locus_tag="PK154_09065"
     CDS             3301..>3703
                     /locus_tag="PK154_09065"
                     /inference="COORDINATES: protein motif:HMM:NF005174.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11247.1"
                     /translation="MTMDLGLGLVLGLSALGSAAGSGTAGMAAIGAWKKNYIQNKPAA
                     FMLVAFAAAPLTQTIYGFIVMNSMVNLAIQGNDLWGIGAFCGAAIGLSAYFQGKCGAC
                     ACDAMGETGKGFGNLMIILGLTETVALLVMAF"
BASE COUNT          962 a          863 c          974 g          904 t
ORIGIN      
        1 acccatgatc gatgtgttta attcactcgt ggaatcccag aaactcccga tattttccat
       61 cgccggggag ccttacaacg agcttctcgc aagaatcggg acccaggcgg aggttgacat
      121 catcattctt gggggaatgg gattgaaata cgacgattat ctcttcttcc gtgacactct
      181 cgaagagcat ggtgcactct cccattcaat cgtgtttatg catacagcag ccgatcccgt
      241 tgttgagtgt ctgctggtcc ccgatatcag ccttgccgtt gcagaaaatt ttgctcttga
      301 aggaaaaagg gtccttgtac tgctgacgga catgagcaat ttctgcgatg cattaaagga
      361 gattgccatc accatgcagc agataccttc aaaccgcgga tatcccgggg acctgtacag
      421 tctcctcgca tcccggtatg aaaaggcggt ggatttcgag accgcaggct cgattacgat
      481 cctcgcggtg accacaatgc cgggagatga cgttacccat ccgattccgg ataacactgg
      541 atatatcacg gaaggacagt tttatctcaa gaatggtgct atcgagccat tcggctcatt
      601 gagccggttg aagcagctcg tgaatgccaa aacaagggat gaccaccgga cgatcatgga
      661 taccatgatc cagctctttg ccgattaccg tgaaacacag gagaaacagt caatgggatt
      721 ccagatgagt gcctgggata tgaagctcct caaatatgga agactcttcg aggaaaaaat
      781 gatgtccctt aaggtgaata tccccctcga gaaagcgctc gacctcggct gggagattct
      841 gcatgattgc ttcttgccag aagagacagg gattaagaga tccataatcg atcaatactg
      901 gccgaaaaaa ccatgaaaat aaaatttacg cagggtgaac tgaaaaaaca gagggatgca
      961 ctgcggcagt acgagaggta cctgcccacc ctgcagctca aaaagcagca gatccagctc
     1021 gaaattcttc aacagcagag ctctctccgg gggagggaaa aaacactgtc cgaaaagata
     1081 cgatcggcag aggaatggtc agcgctcctt cctgaagtcc ctggaataag aatgtggttg
     1141 aagcccgaaa gaatagtcac tggcaagagg aatattgccg gggtcgatat acccatcttt
     1201 gaaaatgttt attttgaacc ggctgagtat aacttgttcc tgatgcctct ctggctggat
     1261 acaggattgg atgccctgaa agaactcacc tctctgcagg aagagatact ggttatccaa
     1321 cgagggatat ctgtcctgaa ccaggaactg cgaatcacaa cccagcgtgt caacctgttc
     1381 gagaaggtaa agatacccga ggcaaaggaa gcaatacgcc taatcaagat atacatcggc
     1441 gatcagatga ccaatgcagt cggccggagc aaaattgcca aaaataagat cgaacaatcg
     1501 gcctttgaag agatagcact atgatcgttc cgatgaaaaa agccactata attttccagt
     1561 caggcaatgc ggacgcaacc gtcgataccg tgcgtaaact gggggttatg caggtagaac
     1621 accagaacat tcctgaaggg ggtaaaattt cggcactcct ggaaaaagtt gccctgatca
     1681 attcctcgat tgatgtcctg aaccagctgc ccatatccga gagaaaaaat cagccgcaag
     1741 gtccgatcag cggtgactgg agggaggttg ccgggagtat catcgaaacg ggccggaagc
     1801 aggaacaact ggaactctcc agaaggaaca ttatcggtca gattaacgaa tgggaacgat
     1861 ggggtgatat tgatcaagcc gaggtccgct cattggctca aaacggcatt cacctcaaat
     1921 tataccaggt tccggtaaga gaactgggaa aattccctga cgatatcgta gtaaagacga
     1981 tatatattgc cggggacatc gcccatgtgg tggcgatatc gcgacgggag tttgaatgcg
     2041 gattcaagga ggtaatcccc ccggagcagg gcttgtcccg gcttaaggag cgacttgccg
     2101 aacataccag gagtatggag atgaataaat ccggaatcct caattccgcc ggattttatg
     2161 atgaccttct ggacattaaa agaagactgg agaaagagat agagtttcaa gaggttctgg
     2221 ccggaatggg cacggaaggg gcactcagct atatcacggg atatgtcccg tctgacttag
     2281 aagattccct gataggggaa gcaaggaaaa acggatgggg catactcatc gctgaacctt
     2341 cccctgatga caatgtaccc accctcctgc gtaatccccg gtgggttgag atgataaaac
     2401 cggtccttgg acttcttggg ctcacgccgg gataccgcga actggatatc agtctcgtgt
     2461 tccttatttt tttcagtata tttttcggca tcctcattgg tgatgccggg tatggacttg
     2521 tctatgttat cataacgttg ctcctatccc tctggttgaa gaagaagatg atgatgaccg
     2581 gagagatgaa gatcaccctc attctcttct atctcctggg ttcctgtgcg attatttggg
     2641 gtgttctgac cggcaccttt tttggccaat ggggattgac cgctctcgtt cccgagctca
     2701 acgactcggc gttcgtgact acgttatgtt tcttccttgg cgcacttcac ctgtcaattg
     2761 cccactgctg gagggctgca ctaaaatacc cctcggtgaa ggcacttgct gacgtcgggt
     2821 atatctgcat tctctggact gcgtttttcc tcgccaacac gttgatcttg ggggatcctt
     2881 ttccctcctt tggattatgg ctagttattg caggaataat actggttatc ctgttcacca
     2941 atcccaaacg ctcgaatatc ttcaagggca tcggagaagg tttggggacg atcgcattga
     3001 gctttatgaa taatatcact gacgtaatct catacattcg tctttttgcg gtgggtatgg
     3061 cgacgctggc agtcgcaaat acgacaaatt atctcgcatc cgggtttggg agtgggatca
     3121 tcgcgggtgt ggcaggagcc gtaattcttt tatccggtca tagcttaaac ctcatcctgg
     3181 gactgatgtc agtcctcgtg cacggtgtca ggctcaatgt gcttgaattt tcagggcatg
     3241 ctaatgtgac atggagtgga actccattcg aaccgctcaa agagtgacag ggaagatatc
     3301 atgacgatgg atctgggatt gggcctggtg cttggcttgt cagcgctggg atctgccgct
     3361 ggatcgggta cggcaggtat ggccgcgatt ggtgcctgga aaaagaatta catccagaac
     3421 aagcctgctg catttatgct cgttgctttt gctgctgccc cgctcaccca gacaatttat
     3481 ggatttatcg tgatgaattc aatggtcaac cttgcgatac aggggaatga cttgtggggt
     3541 atcggagcat tctgcggcgc tgcaatcggc ctgtctgcct atttccaggg caagtgcggg
     3601 gcatgtgcat gcgacgcaat gggagagacg gggaaaggat tcggcaacct catgattatc
     3661 cttggtctga ccgagaccgt tgcattgctc gtgatggcgt ttg
//
LOCUS       DAOOCO010000181         3653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_24310_length_3653_cov_5.282657, whole genome shotgun sequence.
ACCESSION   DAOOCO010000181 DAOOCO010000000
VERSION     DAOOCO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24310_length_3653_cov_5.282657"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="frhB"
                     /locus_tag="PK154_09070"
     CDS             <1..662
                     /gene="frhB"
                     /locus_tag="PK154_09070"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HPW11248.1"
                     /translation="RGTRYNISPNVSFIKEATRSYGLDRIGIVGTPCQMQAVRKGQLY
                     PAGLRDVPDKIALAVGIFCMENFPYQSIKQLVEDHAAMKLESVKKMEIGKGKFWVYGE
                     RGQVVQLPLKVTHKYEQPGCHVCLDYVANLADVSTGSVGSPDGWSTVFVRTKKGDDIW
                     TKAIAAGCFETKSIEQVKPGLELVSKLANEKITKNQKTVEERAKFGVNKALRNPYISP
                     K"
     gene            complement(863..1345)
                     /locus_tag="PK154_09075"
     CDS             complement(863..1345)
                     /locus_tag="PK154_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HPW11249.1"
                     /translation="MKQKEVFQGVPGMLRPFKEYMEKKGIAAGDQVVYYGCPGTCTPF
                     VELLAFAVRSMDLEQVFVPYVDEEKAQKMKLVPDIGMQAGGAVKIVNPIAVVVMGGLS
                     MPNVPVTMDQVKSAVEKHPGAHLVGICFMNMFEKAGWIKAMDFDLLIDAMINPVDVWK
                     "
     gene            1462..1971
                     /locus_tag="PK154_09080"
     CDS             1462..1971
                     /locus_tag="PK154_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPW11250.1"
                     /translation="MKKILVCYSTRYGSTGEIAGMIGEELDSTGYEVTVQPIARVKDP
                     GEYDAVVIGSPLYMGKWLAEARDFVSRFRHSLGERPVAVFSIGYSMKDRTKEHLQSGE
                     DALEAIRIFINPIRTGFFPGRVDPDLMSPADKAVIRLGGVTPGDFREEDLVRTWAKTL
                     AGAMSEEIK"
     gene            complement(2066..2557)
                     /locus_tag="PK154_09085"
     CDS             complement(2066..2557)
                     /locus_tag="PK154_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW11251.1"
                     /translation="MQESRLKIVVFGSYHAGKSTFIQAVDPASRHVDTASGDGTTTVA
                     LDYGKVMMDDVPVHLFGTPGQERFEFARRIIMEGMDAALLLVDCSCAVDDFTKRLYYD
                     LVDSHVPLGLMLNKCDVVESCPAMVRRDLPETRYIYEISARDPSSAREALARFVAGIK
                     ENR"
     gene            2696..3175
                     /locus_tag="PK154_09090"
     CDS             2696..3175
                     /locus_tag="PK154_09090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW11252.1"
                     /translation="MSEVKGHPAGKQVLFETSMGEVVIELYGDMPLTAGNFEKLVASG
                     FYNGIMFHRVIDGFMIQGGCPKGTGTGGPGYVIKDEFTAANNNNRGTISMANAGPNTG
                     GSQFFINLVDNNYLDKNHPVFGKVIRGMDVVDKIGKVKTDRNDRPLSPITITRASVH"
     gene            complement(3478..3606)
                     /locus_tag="PK154_09095"
     CDS             complement(3478..3606)
                     /locus_tag="PK154_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11253.1"
                     /translation="MWSTEFAGFEIVPAGMVKEVVTSIRKRKGLKEQIPTPSDYLA"
BASE COUNT          980 a          905 c          887 g          881 t
ORIGIN      
        1 caaggggaac ccggtacaac atcagcccca acgtctcctt catcaaggag gcaacacgga
       61 gctacggtct cgacaggatt gggatcgtcg gaactccctg ccagatgcag gcggtccgga
      121 aaggccagct ctaccccgcg ggtcttcgtg atgttcctga caagattgcc cttgcagttg
      181 gtatcttttg tatggagaac ttcccctacc agagtatcaa gcaacttgtt gaagaccatg
      241 ccgcgatgaa actcgaatct gtcaagaaga tggagatcgg gaaaggaaag ttctgggtat
      301 acggcgagcg cggacaggtg gtccaattgc cactgaaggt tacccacaaa tacgaacagc
      361 caggctgcca tgtctgcctc gattacgttg caaatctcgc tgatgtctcc accggttctg
      421 tcggaagccc ggacggctgg agcacggtat ttgtgcggac caagaaaggc gatgacatct
      481 ggacgaaagc aatcgctgca ggatgctttg agacaaaatc cattgagcag gtgaaacccg
      541 ggctcgagct tgtctcaaaa ctggccaacg agaagattac caagaaccag aagaccgtag
      601 aagagcgggc aaagtttggc gtcaacaagg cgctcaggaa tccctacatc agcccgaaat
      661 aatactcttc aaacattttt ttttccgtaa gtaaccgatt tttttgtctg ctgcaacgtt
      721 cgtcctttta cccggttatc atttagtgga tatccgtcgc ccatgcaggt ttgaaaaagg
      781 gaagtgagat atccggaaga gaaagtgttc tgatcccaga ccataaaact gctctgtctt
      841 gaaaaggaga gggattcttt ttttatttcc agacatcaac cggatttatc atcgcatcga
      901 ttaaaagatc aaaatccatg gcctttatcc atcccgcttt ctcaaacatg ttcataaaac
      961 agatgccgac gagatgggca ccgggatgtt tctcgactgc cgacttcacc tggtccattg
     1021 tgacgggaac attgggcatc gagagcccgc ccattaccac cactgctatc ggattcacga
     1081 tcttcactgc gccccctgcc tgcatcccaa tatcagggac aagtttcatc ttttgtgctt
     1141 tttcctcgtc tacataggga acaaagacct gttcaaggtc catgctcctg actgcaaacg
     1201 cgaggagctc gacaaacggt gtgcaggtgc ccggacagcc ataatacacg acctgatcac
     1261 cggcggcgat cccttttttc tccatatatt ccttgaaagg ccgcagcatc ccgggaacgc
     1321 cctgaaaaac ctctttttgt ttcatgacca tcactggtat tccattaccg gacccaccgg
     1381 cagccggccg gattaccggg aatgtaaata gggagattct ccaatattaa agtaacattt
     1441 gtacaagcgg ggattttaag cgtgaaaaaa attcttgtct gttattcaac ccggtatggc
     1501 tccacaggag agatcgccgg gatgatagga gaggagctgg attcaacagg atatgaggtg
     1561 acggttcagc ccatagccag agtaaaggat ccaggcgaat acgatgcagt tgttatcggg
     1621 agcccgctct acatgggaaa atggctcgct gaagcacgcg attttgtgag caggtttcgt
     1681 cattctttgg gagaacggcc tgttgctgta ttttccatcg gctactcaat gaaggaccgg
     1741 acgaaggaac atttacagag cggagaagat gcccttgaag ccatacggat attcatcaat
     1801 ccgatcagga ccgggttttt cccaggaagg gtagatccgg acctgatgtc tcccgctgat
     1861 aaggcagtga taaggctagg aggggttact cctggtgact tccgggaaga agacctggtg
     1921 agaacctggg ctaagactct tgcaggagca atgtcagaag aaatcaagta gaacatggat
     1981 tcgcccccgt ttcagggctc ttgggattcc tcaaaggagg gggagaatcc tgtgtgcgag
     2041 gtctgagtga tatatgattc ctcacctatc gattctcttt tattccggcg acgaatcttg
     2101 caagtgcttc ccgtgcgctt gaaggatctc ttgcagagat ctcatagata tatctggttt
     2161 caggcagatc tcttctcacc atcgccgggc atgactctac cacatcacat ttgttgagca
     2221 tcaagccaag agggacgtga ctatcaacga gatcataata gaggcgcttt gtgaaatcat
     2281 cgaccgcaca tgaacaatca accagaagaa gagcagcatc catcccttcc atgatgatcc
     2341 ggcgggcgaa ttcaaaccgt tcctgtcctg gcgtaccaaa aaggtgaact ggaacatcat
     2401 ccatcatcac ttttccgtaa tccaatgcga cagtagtggt accgtcaccg gatgcagtat
     2461 caacatggcg agatgcaggg tcgactgcct gaatgaacgt tgacttccct gcatggtaag
     2521 aaccgaagac cactatcttc agccgtgact cttgcattgt gcagttatgg cgatgatttt
     2581 tttattaact gtttcgatct ttttccctta caaggattta ctcattagcg tttctgccaa
     2641 tggacagtta cacatttcat cggttttatt ccctttcaca ctcaagactt atgccatgtc
     2701 tgaagtgaag ggacacccag ccggaaaaca agtactattt gagacctcga tgggcgaagt
     2761 cgtcatcgaa ctgtacggcg acatgccttt gaccgcgggg aacttcgaaa aactggttgc
     2821 atcgggattt tacaacggga tcatgttcca tcgtgtgatt gatggtttca tgattcaggg
     2881 gggctgcccc aagggaacgg gcaccggagg accgggctac gtgataaagg acgagtttac
     2941 agcggccaac aataataaca ggggtacaat ctcgatggcc aatgcaggcc ccaacaccgg
     3001 cggaagccag ttcttcataa atctggtcga caacaactac cttgacaaaa atcatcccgt
     3061 cttcgggaaa gtcatacgtg gaatggatgt tgttgacaag atcgggaaag tgaaaaccga
     3121 ccggaacgac cgcccgcttt cacccattac tatcacgagg gcatcagttc attgaagggg
     3181 caactcccca ctcaatggtc tcatacatct ttttctctca tggtacgggt taacttcctt
     3241 ccccggttat atagttcatt tcggtcattg cctatacaga gatcacataa tgacaattga
     3301 aaacgatgac ccgctctggc aagaaacctt cataataaat cacggacctc ccagaagaga
     3361 gggaacaatc aattcttcgc ctccataact tcaattttcc tcactgaaga ggctgagaaa
     3421 tcaggagagg gattgaaaat atgaatgcga tcgttataaa aaagaagacc gtatggatca
     3481 ggccagataa tcagacggtg tcgggatctg ttccttgaga ccctttcgct tccgtataga
     3541 ggtcaccact tcttttacca tacctgcagg gacgatctca aatccagcaa actcggtact
     3601 ccacatcgcg cggcctcagt agcagacctg atatcgccgg caaacccgaa gag
//
LOCUS       DAOOCO010000182         3648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_24368_length_3648_cov_4.448928, whole genome shotgun sequence.
ACCESSION   DAOOCO010000182 DAOOCO010000000
VERSION     DAOOCO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24368_length_3648_cov_4.448928"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PK154_09100"
     CDS             complement(<1..709)
                     /locus_tag="PK154_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPW11254.1"
                     /translation="MDIYIDESGDIGLGPKSSPFFVIAALIIHQPLQIQRCFKKIRKN
                     RLKKKLRELPELKFNNSDNVIKRRVLDCLATSDIEFWCSVLRKEQLYDRLRTQPQIVY
                     NYLTGSLIANIYFHYQPFSEFNVIVDKSLNGIHKEQFDQYIIYKTMERHIPITGTLVL
                     PKIIHVDSRRERVFRPQISLLALFIENIARRMTGSFVSSNINVNNYLITLKGRKSGCD
                     PFPTLSHLPQGSLIRFGK"
     gene            complement(1070..1219)
                     /locus_tag="PK154_09105"
     CDS             complement(1070..1219)
                     /locus_tag="PK154_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11255.1"
                     /translation="MTRGKFCSVENLSANAMMPDHYVNAGRGSSILLSEKGKGRLTVL
                     RLVFS"
     gene            1619..1783
                     /locus_tag="PK154_09110"
     CDS             1619..1783
                     /locus_tag="PK154_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11256.1"
                     /translation="MEETHWCSRENRLENFGMIPIALLPCKREACEQWEDGPMKRCVH
                     LVKVERGRRI"
     gene            2255..2551
                     /locus_tag="PK154_09115"
     CDS             2255..2551
                     /locus_tag="PK154_09115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPW11257.1"
                     /translation="MLTVIAKMPIKEGKMDEAIKEMKGLLVHVAKEKGTVLYSLNVET
                     ANPNTLVVVEQYTDQDALDFHSSTPYFKEFFTRASVFLGAAPEIVVLKEIARAQ"
     gene            complement(2686..3201)
                     /locus_tag="PK154_09120"
     CDS             complement(2686..3201)
                     /locus_tag="PK154_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HPW11258.1"
                     /translation="MIPKSPGESVIYAEEQRFRQIWILILVGFIALLAWYSFLLQIVI
                     GEPFGTNPAPDILVILLFVVFGILFPVWFLVMRLEVQVTRTDLRFRMFPLHLHWREFP
                     LDNIVKAEAVTYRPIREYGGWGIRIGMKGWAYNVSGNRGVQITLEGGRSFLLGSLQPE
                     ELAFAIKSGIA"
BASE COUNT          962 a          869 c          896 g          921 t
ORIGIN      
        1 ccttcccgaa acggatgagg ctaccttgag gtaggtggga caaagtaggg aaggggtcac
       61 atccactttt gcggcccttc aaagtaatca aatagttgtt tacattgatg ttcgatgata
      121 caaaagaacc agtcatcctc ctcgcgatat tttcgatgaa cagcgccagc aatgaaatct
      181 gcggcctgaa tacacgttct cgtcgggagt cgacatgaat tatctttggt aatactaaag
      241 tgccggtaat tggaatgtga cgttccattg ttttatagat aatatactga tcaaattgtt
      301 ccttatggat cccatttagt gatttgtcca caatgacatt gaactcggaa aacggttgat
      361 agtggaaata aatattcgca atgagggaac cggtgagata attatagact atctggggtt
      421 gagtccgaag gcgatcatat aattgctctt tccgaaggac tgaacaccag aattcaatat
      481 cagatgttgc caagcaatca agaacacggc gttttatgac attatcagaa ttattaaact
      541 tgagttcagg caattcccga agctttttct tcagacggtt tttcctgatt tttttaaaac
      601 agcgttgaat ttgtaacggt tgatgaataa ttaatgctgc aatgacaaag aacggggaag
      661 atttagggcc taagccaata tcccccgatt catcgatata gatatccacc cgaattcttc
      721 tgcataatgt tgcttaaagg atgtgatttc aggtttattc tttatattct gccaccgagg
      781 gctcaagtac cactactcta tcttcctgag attgaccatg atttcttcat caatccggag
      841 attcatcata aaaagagatt gggcagacga tagggagagg gggatgtgaa tagctgttca
      901 agagtccgtg attatctcca ccctcttcag tcaatgcctc ctgccatgca tcccgggaag
      961 accgtacatg gggtatcgac tcaatcgatc cttatcgaat cattcccgtt gttaccactg
     1021 gtcacctgcg aacatcggca ggaggtccga atccatcaac cttaaagtat tatgagaaga
     1081 ccagccggag aacggtcagc cttcccttcc ccttttcaga caacaggatc gacgaacccc
     1141 tcccagcgtt cacatagtga tcgggcatca tcgcgttcgc acttaaattt tcgacagaac
     1201 agaattttcc cctcgtcatc ttcacggaga aaaatttgtc gatactcaca ccctgtgcat
     1261 gggttcatac ggtaagcccc ctcgctcctc ttggggagca ttgtttcttc agtctccccc
     1321 attccacata cattctcagg gtattatttt tgactgagta tcagctgcct ggccggtcat
     1381 ccatgctgac agagaagtct ggccttcggg tgcaacgggt atcatcttaa aaaaatagca
     1441 atgttctttc actatttcag cacttattcc ctttcagggg atatccctgc tcatagcaac
     1501 gcattggacc accaggttgc ctgtcgaagc aacggatcac cacttccggt gatgtgggac
     1561 ttcactttct ccctgcctat accgcgatgc catatccttg agcatctcac ctcgtatcat
     1621 ggaagagacg cactggtgta gccgggagaa caggcttgaa aattttggga tgataccgat
     1681 cgcccttctc ccctgcaaac gggaggcctg cgagcagtgg gaagacgggc cgatgaaacg
     1741 atgtgttcat ctggtcaagg tagagagggg aaggcggatc taggatgcac cgatgccatg
     1801 gccgatgaca actcaccccc atgggttcca gcggactggc tgaatagcaa cacgaatccg
     1861 ggtgaagtgt tctcgctatg gcaacgaccc ggatgcgtgc gttgtcctgg tgttcatcct
     1921 cgaatgaaaa agagatggca ggggcaagtc aatccaggca ttttctgacc ggccacttcc
     1981 ttcaactttc accaatttcc gcgggcgtgt catcacctca tgctgtccga taaggtgaga
     2041 cgattaccac ttttaccggt cggtatttaa cccggatcac tgcacaaggt gaaagtggta
     2101 ccaccccgat gaaaaaacag agcagtagca tcttcctcgg tatgaatggt actatcagga
     2161 gggggctcga tcatttttta ttgtttgatc aagacagttc tcctgtgttc tgtggatcag
     2221 aagagatagt accaccaagg gaggaggaga agaaatgctg accgttattg ccaaaatgcc
     2281 tatcaaagaa ggaaagatgg atgaagctat caaagaaatg aaggggcttt tagtgcacgt
     2341 ggccaaagaa aaggggaccg tcctgtattc gctgaacgtt gaaacggcca atcccaatac
     2401 cctggtggtg gtcgaacaat acactgacca ggatgccctg gatttccatt cttccacccc
     2461 gtatttcaaa gaatttttca ccagggccag cgtgtttctc ggggcagctc cggaaattgt
     2521 ggtattgaaa gagatagcca gggcacaatg atcggttgct ttcactcatg gtgactgccg
     2581 gtctcctgtc gtttgactgc tgatgcacaa ccgagaaggc caggaccact ctggaaaaaa
     2641 acagaggtat caggtccacg aaccatttcc cggttgccgg aacctttacg caatgccgga
     2701 cttgatcgca aacgccagtt cctctggctg gagcgacccg agcaggaacg atctcccgcc
     2761 ttccagcgtg atctgcactc ccctgttccc cgagacattg taggcccatc ccttcatccc
     2821 tatccgtatt ccccagcccc catactcccg gataggccgg taggtaacgg cctctgcttt
     2881 gacaatgttg tcaaggggga attcccgcca gtggagatgg agcgggaaca tgcgaaaccg
     2941 gaggtcggtc cgggtgacct ggacctcgag tctcatcacg aggaaccaga ccgggaagag
     3001 gatcccgaat accacaaaga gaaggatcac caggatgtcg ggggcagggt tcgtcccgaa
     3061 cggctcccct atcacgatct gcaggagaaa ggaataccag gcaagtaggg cgatgaaccc
     3121 gacgaggatg aggatccaga tctgccggaa ccgctgctct tcagcgtata ttacgctctc
     3181 tccaggggat ttcgggatca tgctacaagg gtcggggttt acgggctaaa ggatttttgg
     3241 tcgggatggg gctcggtata cagtctccga tggtaggttt tttccaagaa tgcccgattt
     3301 atgcaagatg atgatcccac gaacattctt gtgatgccaa attctgtgac cggagccacg
     3361 gtgagcctaa gcatgttctc gttcatggtg ggttaaggct agccggatca atccggggga
     3421 taaggatggc tctgggatac aataactttt gctaggcagg actatttgta caatacaagg
     3481 gctgcgcaaa actattcggg cagaaccatg ttttgtagga gaattatgag taatgattaa
     3541 atactaatac cattatttac ccttttgtat cattataaat tctgatacat tcggatagac
     3601 ctctcaacca tgactgcaga gaagaatgaa acggaagaag agaaggtg
//
LOCUS       DAOOCO010000183         3641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_24432_length_3641_cov_4.893196, whole genome shotgun sequence.
ACCESSION   DAOOCO010000183 DAOOCO010000000
VERSION     DAOOCO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24432_length_3641_cov_4.893196"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK154_09125"
     CDS             complement(<1..318)
                     /locus_tag="PK154_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HPW11259.1"
                     /translation="MIRDFIYKYYIDPIVYGQPYNIVDTLTYALVLILALYLIYRWLS
                     RSKIVQVDRRFILATLPYVVLGGLLRVVEDTGMISPPWQYLLITPLIYFVLFFFTVTA
                     LVVT"
     gene            331..1410
                     /locus_tag="PK154_09130"
     CDS             331..1410
                     /locus_tag="PK154_09130"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HPW11260.1"
                     /translation="MSADRIKVLKQKGTEKSRWMQLPRDVVIGHEVLTHLPAVCSDLR
                     LGKSVLVITGTHTRDLAGEQVIGLLSGQYDVTPFVAGTISMEVIREAEQAAAGKDFVI
                     GVGGGRVIDTAKIVSYNLDRQFISVPTAASHDGIASARASVPMVEGSVSLEAHPPIAI
                     VADTGLIARAPHRLLAAGCADILSNYTACLDWEFAHRLRGEPVSEYAIALSRMTAELL
                     MKNAKTIRPCQEESAWFVTKALVSSGVAMSIAGSSRPGSGGEHKFGHALERLAPGKAL
                     HGEACGIGTIITMYLHGGDWREIRGALREVGAPTTPTELGIPDEIAVEAIITAKEIRP
                     ERFTILDMGISRESAEHLVRMLYAE"
     gene            1418..1864
                     /locus_tag="PK154_09135"
     CDS             1418..1864
                     /locus_tag="PK154_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HPW11261.1"
                     /translation="MVEGKPKVTLIGKELAKNGLEFIYEGSIAECSACKLKSACNNLK
                     KGRRYRIVGVRSVEHTCTVHLNGAQSVEVVESPIPALLPADMSIKNTKIQFECSCNRE
                     LCPNYPFCRPDGIIEREKYIVVNVIGGAPEQCEKGRTLQFVELIPV"
     gene            complement(2112..2975)
                     /locus_tag="PK154_09140"
     CDS             complement(2112..2975)
                     /locus_tag="PK154_09140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPW11262.1"
                     /translation="MHAAGSLVGLAVGDAFGAPLEGLASPTETVREMGYSYRHGRKPG
                     TFTDDTEQALAVARSLVRSRGFSPEDMMAELVQAYRSDPAVYGPTSRRVFDLVLLGMD
                     PRDAARAAHEMNRGSRSNGSVMRGPPIGIWSGGPEVEVYSMACSRLTHYDPVAGACSS
                     FVNRMVSDLCRGREREDAFRRALSRCRDREVAGMLGSFREHDPVSGLDCLLASHASLS
                     VFMEQDTFEETLVAAVNLGGDADTVGAIAGSLAGARYGLSAIPRRWLAALQGLPEVLS
                     TAWQLWHVARG"
     gene            3033..3314
                     /locus_tag="PK154_09145"
     CDS             3033..3314
                     /locus_tag="PK154_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HPW11263.1"
                     /translation="MKKGDSVQKEELLHLHMLMIHIKKYYENITNDEIPTKHYSSLAV
                     SPVHIHKNKKCHKEAILALGEDIVSHLHAQRPAVKVTSEMAPGKVAAEQ"
     gene            3322..>3641
                     /locus_tag="PK154_09150"
     CDS             3322..>3641
                     /locus_tag="PK154_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HPW11264.1"
                     /translation="MSVPDIHREIIDLIFSKHPSPADVTRLKIEVCRNHHLDSVPRNS
                     ALLAAALPHERPALRTLLLVKPTRTLSGVAPDAVMTSPAPCPHGKCLPCPGGPEHPYA
                     SPQS"
BASE COUNT          777 a         1166 c         1066 g          632 t
ORIGIN      
        1 ggtcaccacc agggcagtga ccgtgaagaa gaagaggacg aagtagatca gcggcgtgat
       61 caggaggtac tgccaggggg ggctaatcat cccggtatcc tcgaccaccc gcaggagccc
      121 cccgagaacc acgtagggga gcgtggcgag gatgaaccgg cggtccacct ggacgatctt
      181 cgagcgggag agccaccggt agatcaggta gagggcgagg atcaggacaa gagcgtaggt
      241 gagcgtatcg acaatattgt atggttgtcc atagactatg ggatcaatgt aatatttgta
      301 gatgaaatcc ctaatcattg cctgatctaa atgagcgcgg atcgtattaa agtactgaag
      361 cagaaaggca cggagaagtc ccggtggatg cagctcccgc gggacgtcgt gataggccac
      421 gaggtgctga cccatctccc ggcagtctgc tccgacctcc gcctgggcaa atccgtgctc
      481 gtcatcaccg ggacccatac ccgtgacctt gccggggagc aggtgatcgg cctcctgtcc
      541 gggcagtacg acgttacccc gtttgttgcc ggcaccatca gcatggaggt gatccgggag
      601 gcagaacagg cagcggcagg caaagacttc gtcatcgggg tcgggggtgg cagggtgatc
      661 gacaccgcaa agatcgtctc ctataacctc gaccggcagt tcatcagcgt gcccacggca
      721 gcctcccacg atgggatcgc ctctgcccgg gcatccgtgc cgatggtcga ggggagcgtc
      781 tcgctcgagg cccacccccc gatcgcgatc gtcgccgaca ccggcctgat cgcacgggcc
      841 ccccatcggc tcctcgctgc aggctgcgcc gacatcctct cgaactacac cgcctgcctc
      901 gactgggagt ttgcccaccg gctgcggggg gagccggtca gcgagtacgc gatcgctctc
      961 tcgcggatga ccgccgagct cctgatgaag aacgcaaaga cgatccggcc atgccaggag
     1021 gagagcgcct ggttcgtgac aaaagccctc gtctcctccg gggtggcgat gagcatcgcc
     1081 ggctcctccc ggccgggttc cggcggtgag cacaagttcg ggcacgccct ggagcggctg
     1141 gccccgggaa aggccctcca cggggaggcg tgcgggatcg ggacgatcat caccatgtac
     1201 ctgcatggcg gggactggcg ggagatccgg ggagccctcc gcgaagtggg tgcccccacc
     1261 acccccacag agctgggcat ccccgacgag atcgcggtcg aggcgatcat caccgcaaaa
     1321 gagatccggc ccgagcggtt caccatcctt gacatgggga tctcccggga atcggcagaa
     1381 cacctggtac gcatgctgta tgcggagtga aaggccaatg gtggaaggaa aaccgaaagt
     1441 gaccctgatt gggaaagaac tggcaaagaa cggcctcgag ttcatctacg agggatcgat
     1501 cgccgaatgc agcgcatgca agctgaaatc ggcctgcaac aacctgaaga aagggcgccg
     1561 ctaccggatc gtgggggtac gctccgtcga gcatacgtgc accgtccacc tcaacggggc
     1621 acagtccgtg gaggtcgtgg aatcgccgat cccggccctc ctccctgcgg acatgagcat
     1681 aaaaaatacc aagatccagt tcgagtgctc gtgcaaccgg gaactctgcc ccaactatcc
     1741 cttctgccgg ccggacggga tcatcgagcg ggagaaatac atcgtcgtca acgtgatcgg
     1801 tggcgcccct gaacagtgcg agaaggggcg taccctccag ttcgtcgagc tcatccccgt
     1861 ctgaccggga tatcgctttt tccaatgaaa atcacttcgt aattttcata atggatgccc
     1921 gtatcccgaa atcggggctc ataggaggtt tatatgaaaa tgcctccggc attttcatct
     1981 tttctgcttg tggccctcga aggaatcatg gtatttttct ggattaccca cgggatcgtt
     2041 taaaagggag cagaagaaac tgctgcccca tctcacgggc ggctttgcat tgacgtggat
     2101 tcgatcctct tttacccgcg ggcgacatgc cagagctgcc acgccgttga gaggacctct
     2161 ggaagaccct gcagtgcggc gagccagcgg cgtgggatcg cagaaagccc gtaccgggct
     2221 ccggcaagcg acccggcgat cgccccgacc gtgtctgcat cgccgccgag gttcacggcc
     2281 gcgaccagcg tctcctcgaa ggtgtcctgc tccatgaaga ccgagagcga ggcatgggag
     2341 gcgaggaggc agtcaaggcc ggaaaccggg tcatgctccc ggaacgaccc gagcatcccg
     2401 gccacctccc ggtccctgca gcgggagagt gcacgccgga aggcatcctc cctctccctc
     2461 ccccggcaga ggtcgctcac catccggttc acaaacgacg agcaggctcc cgcgaccggg
     2521 tcatagtggg tgaggcggga gcatgccatg ctgtacacct ccacctcggg gccgccagac
     2581 cagatcccga tcggcggccc ccgcatcacg ctcccgttgc tccggcttcc ccggttcatc
     2641 tcgtgggctg cccgggcggc atcccgggga tccatgccga ggagcaccag gtcaaatacc
     2701 cgccgcgacg tcggcccgta cacggcggga tcagagcggt acgcctgcac cagctccgcc
     2761 atcatgtctt ccggtgaaaa tccccgggag cggaccaggg agcgggcgac cgcaagggcc
     2821 tgctccgtgt catccgtaaa agtgcccggt ttccgcccgt ggcgatacga ataccccatc
     2881 tccctgaccg tttccgtcgg agaggccagc ccttccaagg gcgcaccaaa agcgtcccct
     2941 acagcgagtc ctacgaggga tccggccgca tgcaaaagct ggatataaac ttcaccaaaa
     3001 aggtatatca cgttcccaca ataagaagtg acgtgaagaa aggtgatagt gtgcaaaagg
     3061 aagagttact ccatctccac atgctgatga tccacattaa aaagtactac gaaaacatca
     3121 ccaacgacga aattcccaca aaacattact cctctcttgc tgtttctccc gtccacatcc
     3181 acaagaacaa gaagtgccat aaggaggcca tccttgccct cggagaggac atcgtcagcc
     3241 accttcatgc ccagcggccc gccgtcaaag tcacgagcga gatggcgccc ggaaaagttg
     3301 ccgccgagca ataactcttt tatgagcgtt ccggatatcc accgggagat tattgacctc
     3361 atcttttcca aacacccttc tcctgccgac gtgacgcggc tgaagatcga ggtctgccgg
     3421 aatcaccacc ttgattccgt ccccaggaac tctgcccttc ttgccgcggc cctcccgcac
     3481 gaacggcctg ccctgcgaac cctcctcctc gtcaaaccga cccggacgct ctcaggggtc
     3541 gctcccgacg ccgtgatgac ctcccctgcc ccctgccctc atgggaaatg ccttccctgc
     3601 ccgggaggcc cggagcaccc gtatgcctcc ccccagtcct a
//
LOCUS       DAOOCO010000184         3592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_24937_length_3592_cov_5.106022, whole genome shotgun sequence.
ACCESSION   DAOOCO010000184 DAOOCO010000000
VERSION     DAOOCO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24937_length_3592_cov_5.106022"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="surE"
                     /locus_tag="PK154_09155"
     CDS             <1..634
                     /gene="surE"
                     /locus_tag="PK154_09155"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPW11265.1"
                     /translation="KAYAVGGKPTDSVIIGLYALSLDPVLVVSGINIGENLSYESIMT
                     SGTVGAALEAANQGTPAIAFSLQVEDQGDKFDDPRYHCQSFEDAKTVVREVCTTFLSR
                     KLPRGADIINVNIPSCLTGRYEVTRLSHKLFLTGVEKRLDPRGRPYFWINGPLVNDDE
                     EGTDVHAIRKGNVSLTPITLDCTAPVANEELQSLFSLPSLKYTRCDSEAP"
     gene            658..1368
                     /gene="rpiA"
                     /locus_tag="PK154_09160"
     CDS             658..1368
                     /gene="rpiA"
                     /locus_tag="PK154_09160"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HPW11266.1"
                     /translation="MDEPSLHKETPLTRMKREAGYHAAGMVEDGMVIGLGTGSTTYYA
                     MERLSALIDEGLRIQGVPTSFQTEMLARQLRIPLTTLDTYPELDLAIDGADQVDPSLR
                     LIKGRGAALTREKCVAAAAQSLVIVVDLSKCVDTLAAPVPLEVIPFALQPVILGVRAL
                     GGIPELRTGVRKDGPVITDNGNFILDCSFGTIGEPERLEGILTLMPGVLSCGLFCAFV
                     EKSVIVVGRDGGCRELRP"
     gene            complement(1815..2189)
                     /locus_tag="PK154_09165"
     CDS             complement(1815..2189)
                     /locus_tag="PK154_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW11267.1"
                     /translation="MRARNVMYFTQDEEELADLLIKIGLKRNVARVLVYLAHNSEATS
                     RDIERGTDLRQPEVSLAMAAMIEQDWVENREIKAENKGRPVKIYRLSRPVGEITDTLE
                     KAKREEANLQLSIIQQIRNYIQ"
     gene            complement(2341..2901)
                     /locus_tag="PK154_09170"
     CDS             complement(2341..2901)
                     /locus_tag="PK154_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HPW11268.1"
                     /translation="MKVKPEDSLKIENIVASAKVTDYLDLPALASQIKDAEYNKKRFP
                     GVVIRMQDPKIAALVFGSGKVVLTGAKSIESLSEGLEILGNLLRGLQIDIPQKLTYKI
                     QNIVTSADLGSGINLNKIAVGFNLDRIEYEPEQFPGLVYRLENPKVVVLLFGSGKLII
                     TGGKEPQDAKKAVQKILNDLKNLGLL"
     gene            3212..3373
                     /locus_tag="PK154_09175"
     CDS             3212..3373
                     /locus_tag="PK154_09175"
                     /inference="COORDINATES: protein motif:HMM:NF018715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HPW11269.1"
                     /translation="MADWLLTLGILFIVIAIVLMLLGFIGRVAWTIGKWLIIIFLVLA
                     LLSWVFSFI"
BASE COUNT          772 a         1037 c          960 g          823 t
ORIGIN      
        1 gaaggcctac gctgtcgggg ggaaacccac cgattcggtg atcatcggcc tgtatgccct
       61 ttcccttgat cccgtgctcg tggtcagcgg gatcaacatc ggcgagaacc tctcctacga
      121 gtcgatcatg acctcgggta cggtcggtgc cgcactcgag gctgccaacc aggggacccc
      181 tgccatcgcc ttctctctcc aggtcgagga ccagggcgac aagttcgatg atccacggta
      241 ccactgccag agcttcgagg atgccaagac cgtggtccgg gaggtctgca cgaccttcct
      301 ctcgcggaaa ctcccccgcg gggcggatat catcaacgtg aacatcccct cgtgcctgac
      361 cgggagatac gaggtgaccc gcctctccca caagctcttc ctgaccgggg tagagaaacg
      421 gctcgacccg agaggccggc cctacttctg gatcaacggc ccccttgtca acgatgatga
      481 ggaggggacc gatgtgcatg ccatcaggaa aggaaatgtc tcgctcaccc cgatcaccct
      541 tgactgcacc gccccggtgg caaacgaaga actccaatcg ctcttctcgc tcccttccct
      601 caaatacacc cggtgcgaca gcgaggcgcc atgaattgca atacggaggt atcataaatg
      661 gatgaaccct ccctgcacaa ggaaactccc ctgacccgga tgaaacgcga ggcagggtac
      721 catgctgccg ggatggtgga agacgggatg gtcatcggtc ttgggaccgg ctcaacgacc
      781 tactatgcga tggaacgcct ctctgccctg atcgatgagg gcctccgcat ccagggggtc
      841 ccgacctcgt tccagaccga gatgctggcc cggcagctcc ggatcccgct caccaccctg
      901 gacacctacc cggagctgga ccttgccatc gatggggcag accaggtcga tccctccctc
      961 cgcctgatca aggggagggg ggctgcactg acccgcgaga agtgcgtggc tgcagcagca
     1021 cagagcctgg tgatcgtcgt ggacctctcg aaatgcgtgg ataccctggc ggcccctgtc
     1081 ccgctcgagg tgatcccttt cgccctccag cccgtcatac tcggtgtccg ggcgcttggc
     1141 ggcatcccgg agctccggac cggagtccgg aaagacggcc cggtaatcac cgacaatgga
     1201 aacttcatcc tcgactgctc gtttgggacc atcggtgaac cggaacgtct cgaagggatc
     1261 ctcaccctga tgccgggggt gctctcctgc ggcctcttct gtgcattcgt ggaaaagagt
     1321 gtcatcgttg tcggccggga tggagggtgt cgcgagctca ggccgtgacc ggctcgctct
     1381 gccatcacga gcggacggca cctgattttc atgaggggat agtgcttttt tttctgatca
     1441 gtgccatctg cgtcccccaa attcggtgta tcgggagatt cgggacatta taaaaactgt
     1501 gaaactccga catttgaaaa ccgtgaaact ccggctattt gaaaaccgtg aagctccgaa
     1561 tattgaaaac tcgtgcagtc ttattacagg gaggggaata gtacgccccg gcaggcccaa
     1621 gagacgagat caggtgaccg ggaccttcca ctcctcccgt cgttgatatc cgcggagagt
     1681 gttccggacg aatgggggtc ggagcgtgct gagaccccaa tactgaagag aatatcatca
     1741 tgagagaatt ctgtcgatga aatcaacacg cctttcggga aaaaaagaga gcagtgagag
     1801 ggcactccct gcttctactg gatataattt cgtatctgct ggatgatcga gagctgcagg
     1861 tttgcttctt cgcgctttgc tttttcaagg gtatccgtga tctccccgac cggccgtgac
     1921 agccggtaga tcttcaccgg ccttccctta ttctcggcct tgatctcgcg gttctcgacc
     1981 cagtcctgct cgatcatggc agccatcgcg aggctgactt cgggctggcg gagatccgta
     2041 ccccgctcga tatcgcgtga cgttgcctcc gaattgtggg caaggtagac aagcaccctg
     2101 gctacattcc gcttcagtcc tatctttatg agcaggtctg ccagctcctc ctcatcctgg
     2161 gtgaaataca tcacatttcg ggctctcaac acatgaacac ctgttgtctt attttatatg
     2221 tataaaataa taagttttct atatataaaa atattattat tatatagata gtatttgaaa
     2281 agagaggcta cagaataaaa aggaattcaa aagcaaataa aaattgtggt cactgcagca
     2341 tcacaggagg ccaaggttct tcaagtcatt caggatcttc tgcaccgcct tctttgcatc
     2401 ctggggctcc ttgccaccgg tgatgatcag tttcccggac ccgaagagga gtaccaccac
     2461 ttttggattc tcgagtcggt ataccagccc ggggaactgt tcgggctcgt actcgatccg
     2521 gtcaaggttg aatccgaccg caatcttgtt caggttgatg cccgatccga ggtcggcgga
     2581 ggtgacaata ttctggatct tgtaggtcag cttctggggg atatcgatct gcaatccccg
     2641 gagcaggttt ccaaggatct cgagcccttc agagaggctc tcgatacttt tcgccccggt
     2701 aagtaccacc tttcccgacc cgaagaccag cgccgcaatc ttgggatcct gcatcctgat
     2761 gacaacgccc gggaacctct ttttattgta ctctgcatcc ttaatctggg aagccagtgc
     2821 tggcagatca aggtaatccg tcacctttgc cgaggcgacg atattctcaa ttttgagaga
     2881 gtcctctggc ttgaccttca tatttataaa tgcctcagga cagtatataa acagttgggt
     2941 gatgctacct cgagaaacca gcagcacccc ttctcccggc gtccccccgc aagggaggtg
     3001 tggtggcacc gggtgataat cagggaatct catccgcata ctcattggac ccgatcgcgg
     3061 gagaggggga ggacactggc ctgggggaag actgtttcag cccacgggaa gactgtttca
     3121 acccatgacg gcctaagccc atggaaaggt tgtctctgca ggatctcctg acaaccttta
     3181 ttacccgggg aatgaagacg gaacttattg tatggcagac tggctcctca ctctcgggat
     3241 tctttttatc gtgatcgcca tcgtcctgat gctccttgga ttcatcggga gggttgcgtg
     3301 gaccatcgga aaatggctga tcatcatctt cctggtactg gcactcctgt cctgggtatt
     3361 ctccttcatc tgatcatccg atgggaatta tcctattttc aacacccatg gctgtgtgta
     3421 gattttggca ttccgggcat cccggaccgg cagggagagc cctccgttcc actcttctct
     3481 ttaaatctcc tgttctctga agggatcatc ccctccgctc ttctgaagag aacagaacca
     3541 gcagaacgct tgccacccct ctccctcaga tggcgaggca tggggcctcc cg
//
LOCUS       DAOOCO010000185         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_24985_length_3587_cov_3.568800, whole genome shotgun sequence.
ACCESSION   DAOOCO010000185 DAOOCO010000000
VERSION     DAOOCO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24985_length_3587_cov_3.568800"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK154_09180"
     CDS             <1..237
                     /locus_tag="PK154_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11270.1"
                     /translation="PPAAAEEACAAILEEAAAPPEMVLSRVLTDMEPFVGVDGRIYQL
                     QQGDIVTLPVRNAEVLCDRQIVLAIAAPPAAREE"
     gene            297..1775
                     /locus_tag="PK154_09185"
     CDS             297..1775
                     /locus_tag="PK154_09185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW11271.1"
                     /translation="MAPPALPGPCPSPKNYVHWRGRIYTIYEPSLNLHAWYSLQAQGS
                     TIPCGSSRQGGTGMKVLSFADLALPEPIAKAVKDLGFEEPTPIQSLAIPLLRDGKDVI
                     GQAHTGTGKTAAYGIPIVERVDPAGKTPRALVLCPTRELAIQVSEELRKLARYRRGLI
                     ILPVYGGQPIERQLHALKAGVQVVIATPGRLLDHIDRRSITLSDVGTVVLDEADEMLD
                     MGFRDDIEAILKKTPPGRQTVLFSATMSKEILDLAARYQHDPRTVQVMHDKLSVPKIE
                     QSYFEVRESGKIDVLSKLLDLYNPQLTLIFANTKRRVDEIVSQLQARGYLAEGLHGDM
                     NQAQRERVMGKFREGKIDILIATDVAARGIDVPDVDLVVNFDVPQDPEYYIHRIGRTG
                     RAGKSGRSFTFVSGKEMWKLRDIQRYAKIRIPQQSVPKEHEISTKKAELLGEKVHSLI
                     ETGKLDPETMQVQQIMGEEYTSLEVAAALLSLLSGSGPRSDK"
     gene            1902..3578
                     /locus_tag="PK154_09190"
     CDS             1902..3578
                     /locus_tag="PK154_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW11272.1"
                     /translation="MISVLLVHDNTDLIDSTRSFLEKSGEVRVDTVSSTKQALEILKS
                     RTYDVIVSYYHLPEVNGIEFLPEMNGVELLKYLKSHGNFTPFILYARMGRETLVLEDL
                     NNAHEVRFGKGGDQKKPLTELRDLVIQAVMRKKMERDVTLRTDMLNSILSLTPVWVCQ
                     VRGGVIDWANSTMTSGLGHPEGGLVGKNASAFFASRETFDRASRELTYVIDEQGWGRA
                     TTQLKKSDGTLVHANVMVHAIDQKDPSRGQVLVCEDITEKVLLLDSVKESELRVRELL
                     THSSSLIIKLDTDGTITFFNTFAQVFFGYSEAEIVGKNIIGTLLPAGTGDGHKISGFV
                     SDMGLNGEGYTIRITEMRLRDGQPAWVAWTNKALRDAEGHISEIVCIGHDITDHEHRD
                     RTRISTALWKDRVIANSDVTDEVFETVFNICLEIAKEGREGKKVGTAFVIGDTENVLS
                     KSRQLILNPFAGHRIEDRMITNHDIRENIKELAQLDGAFVIRGDGLIEAAARYITIDT
                     SAVGIAKGLGTRHSSVAGITLVTKAIGIVVSQSGGKISIFRDGQVLQEIG"
BASE COUNT          786 a         1093 c         1085 g          623 t
ORIGIN      
        1 cctcctgccg cagctgagga ggcctgcgca gccatcctcg aagaggcggc ggctcctccc
       61 gagatggtcc tctcgcgggt gctcaccgac atggagccgt tcgtgggcgt cgatggaagg
      121 atctaccagc tgcagcaggg cgacatcgtg accctcccgg tgcggaatgc cgaagtcctc
      181 tgtgaccggc agatcgtcct tgcgatcgca gcacctcccg ctgcacggga agaataatgc
      241 ggcatccctg ctgaacacga ctccctcttt tcgcaggcag gcagcccgga ctgccaatgg
      301 caccccctgc tcttccgggt ccgtgcccct ccccaaagaa ctatgtgcac tggaggggga
      361 ggatctatac aatttatgaa ccctctctta acctccatgc ctggtactct ctccaggcac
      421 agggaagtac aataccatgc gggagcagca ggcagggggg aacgggcatg aaagtcctct
      481 cgtttgccga cctcgccctc ccagaaccga tcgcaaaagc ggtaaaagac ctcggtttcg
      541 aggaacccac ccccatccag tcccttgcaa tccccctgct ccgggacggc aaggatgtca
      601 tcgggcaggc ccataccggg accggcaaga ccgctgccta cgggatcccc atcgtggagc
      661 gggtggaccc ggccggaaag accccccggg cgctcgtgct ctgcccgacc cgcgagctgg
      721 ccatccaggt ctccgaggag ctccggaagc ttgcccggta ccggagagga ctgatcatcc
      781 tcccggtata tggcgggcag cccatcgagc ggcagctcca tgccctgaag gctggcgtcc
      841 aggtggtgat cgcgaccccg ggccggctgc tcgaccatat cgacaggagg tcgatcaccc
      901 tctcagacgt cggaaccgtg gtgctcgacg aggcggatga gatgctcgac atgggattcc
      961 gggacgacat cgaggcgatc ctgaaaaaaa cccctcccgg tcgccagacc gtgctcttct
     1021 ctgccaccat gtcaaaggag atcctcgacc tcgcggcccg gtaccagcac gacccgcgga
     1081 ccgtccaggt gatgcatgac aagctctcgg tcccgaagat cgaacagtcc tacttcgagg
     1141 tgcgggagag cggaaagatc gatgtcctct caaaactcct cgacctgtac aacccgcagc
     1201 tgaccctgat ctttgccaac acgaaacgtc gcgtggacga gatcgtctcc cagctccagg
     1261 cccggggcta ccttgccgag gggctccatg gcgacatgaa ccaggcccag cgtgagcggg
     1321 tgatgggtaa gttccgcgag gggaagatcg atatcctgat cgccaccgat gtggccgcga
     1381 gagggatcga tgtccccgat gtcgacctcg tcgtcaactt cgatgtaccg caggaccccg
     1441 aatattacat ccaccggatc gggcggaccg ggagggccgg caaatcgggc cggtctttca
     1501 cgttcgtgtc aggcaaggag atgtggaagc tccgggacat ccagcggtac gcaaagatcc
     1561 ggatcccgca gcagtccgtg ccaaaggagc acgagatctc tacgaaaaag gccgaactcc
     1621 tcggcgaaaa ggtccacagc ctgatcgaga ccgggaagct cgatcccgaa accatgcagg
     1681 tgcagcagat catgggggag gagtacactt ccctcgaggt ggctgcagca ctcctctccc
     1741 tcttgtccgg cagcgggcca cggtcggaca aatgagcatt tgcgggcaca gcacgccaaa
     1801 cggaacggaa aattgcgggc aaatggcctt tcagattcaa taattattta tttgataacc
     1861 cacctacgct atcttgctat ccccgtagtg gagaagagag gatgatttcc gtactactgg
     1921 tgcatgacaa taccgacctg atcgacagca ctcgttcatt ccttgaaaag agcggggagg
     1981 tccgggtcga caccgtctcc tccacgaaac aggcactcga gatcctgaag agccggacct
     2041 acgacgtcat cgtctcctat taccacctcc ccgaggtgaa cgggatcgag tttctccccg
     2101 agatgaacgg ggtggaactc ctcaagtacc tcaagtccca cgggaatttc accccgttca
     2161 tcctctatgc ccggatgggg agagagacgc tggtgcttga ggacctgaac aacgcacacg
     2221 aggtcaggtt cgggaaaggg ggagaccaga aaaagcccct gaccgagctt cgtgacctcg
     2281 ttatccaggc ggtgatgcgg aagaagatgg agcgcgatgt caccctcagg accgacatgc
     2341 tcaactccat cctgtcgctc acccccgtct gggtctgcca ggtccggggc ggtgtcatcg
     2401 actgggcaaa cagcaccatg acctccgggc tcggccatcc ggagggaggg cttgtcggga
     2461 agaacgcctc ggcctttttt gccagccgcg agacctttga ccgggcatca cgggagctga
     2521 cgtacgtcat cgatgagcag gggtgggggc gtgccacgac ccagctgaag aagagcgacg
     2581 gcacgctcgt ccatgcaaat gtcatggtcc atgccatcga ccagaaggac ccatcgcggg
     2641 gccaggtcct ggtctgcgaa gatatcacgg aaaaggtcct gctcctcgac tcggtgaagg
     2701 agagcgagct ccgggtccgc gagctcctca cccactcaag cagcctgatc atcaagctcg
     2761 acaccgacgg gacgatcacc ttcttcaaca ccttcgccca ggtcttcttc gggtactcgg
     2821 aggccgagat cgtggggaag aatatcatcg ggaccctgct cccggcaggg acgggggacg
     2881 ggcacaagat ctccgggttt gtctccgaca tgggactgaa cggagagggc tataccatcc
     2941 ggatcacgga gatgcgactc cgggacggcc agccggcctg ggttgcatgg accaacaagg
     3001 cactgcggga tgcggaaggg cacatctcgg agatcgtctg catcggtcat gacatcaccg
     3061 accacgagca ccgggaccgg acccgcatca gcacggccct ctggaaggac cgggtgatcg
     3121 caaactcgga tgtgacggac gaggtctttg aaacggtgtt caatatctgc cttgagattg
     3181 caaaggaagg acgggaagga aagaaggtcg ggactgcgtt tgtcattggc gatacagaaa
     3241 atgtcctctc caagtcgcgg cagctcatct taaacccgtt tgcagggcac cggatcgagg
     3301 accggatgat cacgaaccat gatatccgcg agaacatcaa ggagctggcg cagctggacg
     3361 gggcattcgt gattaggggg gacgggctga tcgaagcagc tgcccggtat atcaccatcg
     3421 acacgagtgc cgtcggcatc gcaaaagggc tgggcaccag gcattcgtcg gttgccggta
     3481 taaccctcgt cacaaaagcc atcgggatcg tcgtctcgca gagcgggggc aagatctcga
     3541 tcttcaggga cggccaggtg ctccaggaga tcgggtgaac cgcggtg
//
LOCUS       DAOOCO010000186         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_25029_length_3583_cov_4.702381, whole genome shotgun sequence.
ACCESSION   DAOOCO010000186 DAOOCO010000000
VERSION     DAOOCO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25029_length_3583_cov_4.702381"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="hisG"
                     /locus_tag="PK154_09195"
     CDS             complement(<1..612)
                     /gene="hisG"
                     /locus_tag="PK154_09195"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW11273.1"
                     /translation="MINIALPKGSLESQTLQLFKEADLEVKRGDRDYNPIIHDSRIGK
                     VKILRPQEIPKYVEMGYFDMGISGLDWVKETGARVREVAPLSYSKTGEGTVKIVVAVH
                     RDEPIDEVTRIRPHSRVTTEYPEITKEYFGRIGIPVQLFHSFGASEAKVPDLMDVVVD
                     LTETGTTLRRNGLKIIGQIMESYTVIIANRESFEDVAKRRAIEE"
     gene            complement(731..1978)
                     /locus_tag="PK154_09200"
     CDS             complement(731..1978)
                     /locus_tag="PK154_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPW11274.1"
                     /translation="MVRIFPFVAVRPGRDAAPQVAAVPYDVVTADEARRIIRDNPMSF
                     LRVSRPDADLPECAPTDPGVYSSARDRFTALISSGVLARDPGPGMYVYRVITSNDTYT
                     GLCCCLHVDDYKSGIIRRHEQTRYDKEEDRTRHIDTVNAHTGPVVLIYRDRAGLARYI
                     TTLVSGSPDAEVRWNDGSVHQIYRISDSAVVALLEEHFSQVGRVYIADGHHRAKSAVN
                     VAGMRRDSGVDIGEAERFMGVLFSDSGVRIHGYSRLVHDLGGRSPGEFMKEIAQYCLV
                     LPYGKVNNGSFQIPPHSDRPHVFHMYLTGLWYECILKETAGSDLIGSLDVSLLQHQVL
                     EHILGITDPRGDARLQYLGGARPVADLEAMVDRGDYAVAFSMQPVQVSTVLSIADANG
                     IMPPKSTWFEPKLLSGLVIHTLS"
     gene            2051..2512
                     /gene="rimI"
                     /locus_tag="PK154_09205"
     CDS             2051..2512
                     /gene="rimI"
                     /locus_tag="PK154_09205"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPW11275.1"
                     /translation="MGDIITIRRAKPEDIPAVSEIERVSFPDPWSEGILAETLGYFSK
                     TFFVAIFRRSIIGFIAGGLEDTGEEVYGHICNLAVTPQFRSMGVGRMLVRREEHQFAI
                     ELATGVQLEVRLSNYAAQQFYRKIGYREVFHIDRYYANGEDAVVMMKWFRF"
     gene            complement(2509..>3583)
                     /gene="hmgA"
                     /locus_tag="PK154_09210"
     CDS             complement(2509..>3583)
                     /gene="hmgA"
                     /locus_tag="PK154_09210"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HPW11276.1"
                     /translation="AVGAYTIPVDRVAKKNCENLIGAIQIPVGIAGPIAIHGEYAEGS
                     FFLPLATTEGALVASVNRGCSAITQAGGAEVRIFRDGMTRAPLFAATGVVHARAVVDY
                     VHSHQDEIAAAAESTTSHGKLTGITCSVAGTSVFVRLEFDTKDAMGMNMVTIASEKAA
                     QLIASETGAHLIALSSNVCTDKKPAAINMVMGRGRTAAAGVLLTDELIRHVLKTDAAT
                     LAEVNLRKNLVGSARAGSLGYNAHAANIIAALFIACGQDPAHVVEGSLAVTTVDPAPG
                     GVYVSVTLPSLPAGTVGGGTGIATQQECLRLLGVAGGGDPPGSHARKFAEIIASAVLA
                     GELSLLGALSAQHLARAHKALGR"
BASE COUNT          797 a         1012 c          945 g          829 t
ORIGIN      
        1 ctcttcgatg gcacgacgtt ttgccacatc ttcgaaactt tcgcggttgg caatgatgac
       61 ggtatacgac tccatgatct ggccgataat cttgaggcca ttccttcgca gcgtggttcc
      121 ggtttccgtg aggtcaacca cgacgtccat caggtccggg accttagcct ccgatgcacc
      181 gaacgaatgg aagagctgga ccggtattcc gatccttccg aagtattcct ttgtaatctc
      241 gggatactcg gtggttaccc tgctgtgtgg gcgaatcctg gtgacctcgt caattggttc
      301 atccctgtgg accgccacca cgatcttaac cgttccttct cctgtctttg aataggagag
      361 cggggctact tcccgcaccc gtgcccctgt ttccttcacc cagtcgagcc ccgagatacc
      421 catatcaaaa taccccatct cgacatattt tgggatctcc tgcgggcgga ggatcttcac
      481 cttgcctatg cggctgtcat gaatgatcgg gttgtaatcc cggtcacccc gcttcacttc
      541 aaggtctgct tccttgaaga gctggagggt ctgggactcc aggctgcctt tgggaagtgc
      601 gatattaatc atggcataca atgtttgaca gctgagagaa aaaacatccc tgataattta
      661 cagcatcatt tcttacgcct ctggatctcc aggtctcctt tgtttgcttt catgactatg
      721 tgaaaaggga tcaggagagg gtatgaataa caagcccaga gaggagtttg ggttcgaacc
      781 atgttgattt cggcggcata atgccgttag catccgcgat tgaaagcact gtactcacct
      841 gaaccggctg cattgagaat gctactgcat agtctcccct gtcaaccatc gcctcaaggt
      901 cagcgaccgg ccgggcgcca ccgagatact ggaggcgcgc atcgccacgg ggatctgtaa
      961 tcccgagaat atgctccagg acctggtgct ggaggagcga cacatcaagt gagccgatca
     1021 gatcggatcc agctgtttcc ttgagaatgc attcgtacca taatcccgtc aggtacatat
     1081 gaaagacatg gggacgatcg ctatgcgggg gaatctgaaa agaaccatta ttgaccttcc
     1141 cataagggag cactaaacaa tactgagcga tctccttcat gaactcgcca ggagaacgtc
     1201 ccccgaggtc atgcacaagc cggctgtagc catggatcct gaccccggaa tcggagaaga
     1261 gcactcccat gaatcgttct gcttcaccga tatcaacccc cgaatccctc cgcattcctg
     1321 ccacgttcac agcggatttc gcacggtgat gtccatctgc aatgtatacc ctgcccacct
     1381 gtgaaaaatg ctcttcgagg agggcgacca ctgctgaatc ggagattcga tagatctggt
     1441 gaacagatcc atcgttccag cgcacttctg catcagggct tcctgagaca agagtagtaa
     1501 tataacgggc gagtcctgct cgatcgcggt aaatgagtac aaccgggccg gtatgggcat
     1561 tcaccgtatc gatatgccgt gtccgatcct cctccttgtc ataccgggtc tgttcatgtc
     1621 tcctgatgat ccctgatttg taatcgtcga catggagaca gcagcagagc ccggtgtatg
     1681 tgtcatttga tgtgatgacc cggtagacgt acatcccagg acccggatca cgtgccaaca
     1741 cacctgatga tatcagtgca gtgaaacgat cccttgcaga ggaatatacc ccaggatctg
     1801 tcggagcaca ttccggaaga tcggcatccg gacggctgac acgtaaaaaa ctcatcggat
     1861 tgtcccggat gatcctccgt gcctcatcag cagttactac atcgtagggc accgcagcca
     1921 cctggggggc agcatcccgg cccggccgca ctgccacgaa aggaaagatt ctcaccatga
     1981 agattcccgg ctctctttat tcatttcctc tgacagtata ttattccagt gacacccata
     2041 gtatgtggca atgggcgaca ttatcacaat acggcgggca aagcctgaag atatacctgc
     2101 tgtttctgag atcgagaggg tctcttttcc ggatccctgg agtgagggga tccttgccga
     2161 gacacttggg tacttctcaa agaccttttt tgtcgccatc ttcaggagat cgattattgg
     2221 gttcatcgct gggggacttg aagacacagg ggaagaggta tatgggcaca tctgtaacct
     2281 tgcagtcaca ccgcaattcc ggagcatggg agtgggacgg atgcttgtca ggagagagga
     2341 gcaccagttt gccatcgagc tcgcaacggg tgttcagctg gaggtccgac tctccaacta
     2401 cgcagcccag cagttttacc ggaagatcgg ataccgagag gtcttccaca tcgaccgtta
     2461 ttatgcaaac ggtgaagacg ccgttgttat gatgaaatgg ttccggtttt agcggccaag
     2521 agccttgtgc gcccgggcaa gatgctgggc gctgagcgct ccaagcagcg agagttcacc
     2581 ggcaaggact gcagatgcga tgatctctgc gaatttccgc gcatgggaac ctggcggatc
     2641 tccccctccg gcaaccccca gcagccggag gcactcctgc tgtgtcgcaa ttccggtacc
     2701 ccctccgact gtaccggccg gtaacgaggg aagcgtaaca gatacataca ctcccccggg
     2761 agccggatcc acggttgtca ctgccaggct cccctccacc acatgggctg ggtcctggcc
     2821 gcaggcgatg aagagcgcag ctatgatgtt ggcagcatga gcattgtacc caagcgagcc
     2881 tgctcttgcc gatcccacaa gatttttacg gaggttcact tctgcaagag tggcagcgtc
     2941 cgtctttaac acatggcgga tgagttcatc agtgagcagc actccggcag ctgccgtccg
     3001 cccccggccc atcaccatat tgatggcagc tggtttcttg tccgtgcaga cattgctgga
     3061 cagtgcgatc agatgtgcgc cggtctcgga tgcgatcagt tgtgccgcct tttcgcttgc
     3121 aatggtcacc atgttcatgc ccattgcgtc cttggtgtca aactcgagtc gtacaaagac
     3181 gcttgttccc gcaaccgaac aggtgatccc cgtcagtttt ccatgagagg tagtactctc
     3241 cgcggcggca gctatctcat cctggtgcga atgtacatag tcaacaacgg cgcgtgcgtg
     3301 gaccacgcca gtcgcagcaa ataacggtgc acgggtcata ccgtccctga aaatgcggac
     3361 ttctgcccct ccggcctggg taatggcgct gcatcccctg ttgaccgaag cgaccagcgc
     3421 cccttccgtt gtagcgaggg ggaggaagaa cgaaccttcc gcatactctc cgtgtatggc
     3481 tatgggtccg gcaatcccta ccgggatctg gatggcgccg atcagattct cacagttctt
     3541 ctttgccaca cggtcaaccg ggatggtata cgcacctacc gca
//
LOCUS       DAOOCO010000187         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_25401_length_3545_cov_4.686533, whole genome shotgun sequence.
ACCESSION   DAOOCO010000187 DAOOCO010000000
VERSION     DAOOCO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25401_length_3545_cov_4.686533"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /gene="ilvN"
                     /locus_tag="PK154_09215"
     CDS             <1..257
                     /gene="ilvN"
                     /locus_tag="PK154_09215"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPW11277.1"
                     /translation="ERELALIKVTAEPGTSRAEVMQLATIFRAQIIDVAAKTIILSIT
                     GDTEKIDAFEKLLRQYGVKELVRTGRIAMLRGSKVLKGGK"
     gene            complement(884..1339)
                     /locus_tag="PK154_09220"
     CDS             complement(884..1339)
                     /locus_tag="PK154_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HPW11278.1"
                     /translation="MGLEDYKGKRNFQTTPEPEGEGRASPRSSRIFVVQEHHASHLHY
                     DLRLESEGVLRSWAVPKGIPEKPGQRHLAVETEDHPLEYAEFEGTIPEGEYGAGAVKI
                     WDSGIFEPLVWEKDKIEMVMKGDRLSGRYVLVRFKRAGEKDWLLFRAKD"
     gene            complement(1351..2178)
                     /locus_tag="PK154_09225"
     CDS             complement(1351..2178)
                     /locus_tag="PK154_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPW11279.1"
                     /translation="MKKDAAGAAPSSRRAFWTGAISIGLVNIPIRLTVLVKDHTIQFH
                     LLHKKDGQPVRYERVCTRDNEVVPWEEIVRGYEVREGEFIVLEKEEIDAVRPESDRRI
                     RIDRFINVLSIDPVYYDRTYLLLPDGPADAYALLRETFRTMGRAGIGRITLRTKEYPV
                     LVREYQDALILTTLHYPDEVTDPGQIEVLSAIRVPGKKELALAEKIVSDLSGEFEIGD
                     YHDTYRERIEALIQSKMAGTTYHVEKPKAAEVRDLMSALEETLAQLKAERASAKGSE"
     gene            complement(2175..3050)
                     /gene="ligD"
                     /locus_tag="PK154_09230"
     CDS             complement(2175..3050)
                     /gene="ligD"
                     /locus_tag="PK154_09230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPW11280.1"
                     /translation="MPEGKRLGRVVLTNTSRVMYPELNLTKRDVVEYYLRIAPRILPH
                     LGNRALVLQRFPDGVSAPGFYEKDAPAGTPEWVHLYRHHSVSAGRDLHYIVCDSPDTL
                     IWLANLAAIELNIPLARLDDPESPDLLLFDIDPEPPAGFREGVTVALALRDMLFDLGL
                     FPFVKTSGKKGLHVVIPLAGGYRFSHTRNFVHAVGSLLARESPLVVSELTHTHDPGKV
                     FIDYLQNSAWKTMIAPYSLRATGTATVSAPVSWDDLSRGIDPGDFTLQTTRAEDADPW
                     LDLYTSPQRLPEVVR"
     gene            3170..>3545
                     /locus_tag="PK154_09235"
     CDS             3170..>3545
                     /locus_tag="PK154_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW11281.1"
                     /translation="MKTAILGGGLSGLTLARFLHERGEEIVVLEAEPEVGGLCRSRRE
                     GGFCFDTGGSHIIFSRDEEVLRFMLSVLGGNRAVRKRNTRIFYKDRFVKYPFENGLSE
                     LPKEDLYFCLHEYIKTLITSERG"
BASE COUNT          785 a          999 c          959 g          802 t
ORIGIN      
        1 tggagcggga acttgccctg atcaaggtga ccgcggagcc gggtacgagc agggctgaag
       61 tgatgcagct cgccacgatc ttccgtgccc agatcatcga cgtggctgca aagaccatca
      121 tcctctcgat caccggagac acggaaaaga tcgatgcgtt cgagaagctc ctccgccagt
      181 acggggtaaa ggagctcgtg aggactggcc gaatcgcgat gctccgtggt tcaaaggtgc
      241 tgaaaggcgg gaaataacgg aaattttctt tttttacggc atcacttctc aaggatcagt
      301 attcactaat cgctgactat catcctgaag atattctggg acgcccaaga cttcagacag
      361 tccagtttag gaatccagga cgcttcacat cagataattt gtttgatcca gtcgtttgtg
      421 tatatttcat atagtttata taaaataaca tatatattat ggatccactt ctcaaacgat
      481 catttcgatc cctggtcatc actaccaaac aaagcccatt acggtctcct ttttccggtc
      541 ttttccggac tggagggtat tccaatggaa atggcgtcca ccactatgga aaaaaccttt
      601 agaatcgaac caattttgat ttcgtgtgtt ttaatgcctt aaaaaatctc ctctttatcg
      661 cgtttctgtt caaggcttac tgggggcagt atgctgaccg atctttcctc agaaactccg
      721 ctataacgct tctattgagg ttttataaaa taagcgattt taaggctttt ttcgcctgaa
      781 aagccactat gagaggatat agagagagac aagagggata gggggagaga aatgacccca
      841 gtcttgcagt accatgggac agacgagagg cgagatcccc gtttcagtcc ttcgccctga
      901 agaggagcca gtccttctcc cctgctcttt tgaaccgcac caggacatag cggccggaaa
      961 gccggtctcc tttcatcacc atctcgatct tgtccttctc ccagaccagc ggctcaaaga
     1021 tccccgagtc ccagatcttc accgctcccg caccatactc gccttccggt atggtcccct
     1081 cgaattccgc atactcgagc gggtggtcct cggtctccac ggccaggtgc cgctgcccgg
     1141 gcttctccgg gatccctttc gggaccgccc aggatcggag tacgccctcc gactccagcc
     1201 gaaggtcata atgaaggtgc gatgcatgat gctcctgcac gacaaagatc ctggaggagc
     1261 gtggtgaagc cctgccctcg ccttcaggct caggggtagt ctggaaattc cgctttccct
     1321 tgtaatcctc aagccccatg aacgatcacc ttattccgat cctttcgcag aagcccgttc
     1381 agccttcagc tgggcaaggg tctcctcaag cgcagacatc aggtccctga cctccgcagc
     1441 tttcggcttc tcgacatgat aggtcgttcc tgccatcttg gactggatca gggcttcgat
     1501 ccgttcccgg tacgtatcat ggtagtcccc gatctcgaac tccccggaga ggtcagagac
     1561 gatcttctca gcaagagcga gctctttttt tcccgggacc cggatggcgg aaaggacctc
     1621 gatctgcccc ggatcggtca cctcatccgg ataatggagg gtggtgagga tcagggcatc
     1681 ctggtactcc ctgaccagca cgggatactc cttggtccgg agcgtgatcc tgccgatacc
     1741 tgcccgtccc atcgtgcgga aggtctcccg gaggagggca taggcatcag caggcccgtc
     1801 ggggagcagg aggtaggtcc ggtcataata gaccgggtcg atcgagagaa cattgatgaa
     1861 ccggtcgatc cggatcctcc ggtctgattc gggcctcacc gcatcgatct cttccttctc
     1921 cagcacgatg aactccccct ccctgacctc gtagcccctg acgatctcct cccatggcac
     1981 cacctcgtta tcccttgtgc agacccgttc gtacctgacg ggctgtccat ccttcttatg
     2041 caggaggtgg aactggatgg tatgatcttt caccaggacc gtaagcctga tggggatatt
     2101 cacgaggccg atgctgatag cccctgtcca gaacgccctt cgtgaggagg gcgctgcacc
     2161 ggcagcatcc ttcttcatct taccacctcg ggcagtctct ggggggaagt gtacaggtcc
     2221 aaccagggat ctgcatcctc tgcccgggtg gtctgtaggg taaaatcacc gggatcaatt
     2281 ccccgggaaa ggtcatccca ggagacagga gcggagacgg ttgccgttcc ggttgcacgc
     2341 aggctgtagg gggcgatcat ggtcttccat gcagaattct ggagataatc gataaagacc
     2401 tttccgggat catgggtgtg ggtgagctcg gatacgacca gcggcgactc ccgtgcgagt
     2461 aacgaaccca ccgcatggac aaaattcctc gtatggctga accggtatcc gccggcgagg
     2521 gggatcacca catgaagccc cttctttcct gatgtcttta caaaagggaa aagcccgaga
     2581 tcgaagagca tatcccgcaa tgcaagggca acggtcaccc cttccctgaa tcctgcaggg
     2641 ggttccggat cgatatcgaa gaggaggaga tcgggagact cagggtcatc cagccgtgca
     2701 agggggatat tcaattcgat cgcggcaagg ttggccagcc agatgagggt gtccggggag
     2761 tcgcagacga tatagtggag atctctcccg gcagagaccg aatggtggcg atagagatgg
     2821 acccattccg gcgtccctgc cggggcatcc ttctcataga atcccggggc actcaccccg
     2881 tcaggaaacc gctggagcac cagcgccctg ttcccaagat gggggaggat ccggggtgcg
     2941 atgcgaagat agtactccac cacatcacgt tttgtcaggt tgagttcagg gtacatcacc
     3001 cgcgaggtat tggtgagcac cactcttcct agtctcttcc cttccggcat ccattcaccc
     3061 ctcagctgtg atcgttctct tcacgctcac gggccttaaa caatcgcacg gaaatgcctg
     3121 actgccggag gtcaaccata tataaattcg gaactgtaat gtatacgatg tgaaaaccgc
     3181 gattctcggg gggggacttt ccggcctcac cctcgcccgc ttcctgcatg aacgtgggga
     3241 ggagatcgtt gtcctcgaag cggaaccaga agtcgggggc ctctgccggt cgcggcggga
     3301 gggggggttc tgttttgaca cgggaggatc ccatatcatc ttctcaaggg atgaggaggt
     3361 cctccggttc atgctctcgg tcctcggagg gaaccgggcg gtccggaagc ggaataccag
     3421 gatattctac aaggaccggt tcgtgaagta cccgtttgag aacggcctct ccgaactccc
     3481 gaaagaggac ctctatttct gcctccatga atacataaaa accctgatca cttcggaaag
     3541 agggg
//
LOCUS       DAOOCO010000188         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_25469_length_3538_cov_4.589722, whole genome shotgun sequence.
ACCESSION   DAOOCO010000188 DAOOCO010000000
VERSION     DAOOCO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25469_length_3538_cov_4.589722"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..661
                     /locus_tag="PK154_09240"
     CDS             2..661
                     /locus_tag="PK154_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HPW11282.1"
                     /translation="MGFPGSGLVGSIALQYMVDQLEFTQIGSMNSKYFPPLAMMTRGV
                     INVPVRVYEKENLAAIVADIPIHPNICYEVANGIMEWLMPFKIKEIVTIAGIITNEME
                     KRVFGVATSEKALQRIQDSTIILPIGSISGIASSFLTECRIRNVDAYGLLGETINAPD
                     PRASAATIEVLNKMYGLTLDIQPLLDQAEEIESTMHKLAEQVQTAEATPKKEQQLPMY
                     G"
     gene            668..1060
                     /locus_tag="PK154_09245"
     CDS             668..1060
                     /locus_tag="PK154_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HPW11283.1"
                     /translation="MKCFALTGISPDVIQELHKGRPRTLELQSAHNVVTLAGLDPGAA
                     VFMTSVDMEDLDPGDTGIIVEIIAISITMKRMMEFTQGYHFEERERMSARIKVRCVGS
                     SIVKSVTHEGIMHPVSVDVVKSACYHAG"
     gene            1237..1311
                     /locus_tag="PK154_09250"
     tRNA            1237..1311
                     /locus_tag="PK154_09250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1272..1274,aa:Arg,seq:tct)
     gene            complement(1342..1791)
                     /locus_tag="PK154_09255"
     CDS             complement(1342..1791)
                     /locus_tag="PK154_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HPW11284.1"
                     /translation="MSEIVLTEDCMRLISQFERLTGAGSRDCVIDDRNNRLIFVVNPG
                     EMGLAIGKKGASIKKAMEVMGKKIEVVENSPSPEQFLKNCFLPAQVVTVGFETEDEGR
                     IAHIEVRQEDRGIAIGKEGKNIFKAKKLALRQHNISDVTLVTEEEET"
     gene            complement(1795..2085)
                     /locus_tag="PK154_09260"
     CDS             complement(1795..2085)
                     /locus_tag="PK154_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HPW11285.1"
                     /translation="MDFNVSLRRAIKTGDVILGQNQTEQCINEGKAQMVVLAGNCPET
                     FKNLLSSREGLFIYTYEGSSVQLGKACGKPFMVSALAVVNPGESDLLNLKRA"
     gene            complement(2096..3259)
                     /gene="rpoA2"
                     /locus_tag="PK154_09265"
     CDS             complement(2096..3259)
                     /gene="rpoA2"
                     /locus_tag="PK154_09265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HPW11286.1"
                     /translation="MQTDHEAKIDEADLPDKTKDQLKKYLEGKEITDTQFDQILNRVV
                     NEYQNTRIEACEAVGIIAAQSIGEPGTQLTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     KEPSTPTMTIFLEPEFGVDRDRAREVSWQIEAAPLHEFGDITIDMENMQIVVHLNTAV
                     CEKRKIAPLSILEVAPKKIKERRHYRDFEFEMDEKTATIIFMPKDRESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKESGEYILYTEGSNIKDVFEVEGVDTTRTRTNNISEISQV
                     LGIEAARNAIIHEALSTLGEQGILVDVRHIMLVADMMCMEGEVKQIGRHGIAGEKESV
                     LSRAAFEVTVNHLLDAAVANEVDELSGVTENVIVGQPIQLGTGDVKLIAKSLN"
     gene            complement(3261..>3538)
                     /locus_tag="PK154_09270"
     CDS             complement(3261..>3538)
                     /locus_tag="PK154_09270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HPW11287.1"
                     /translation="LNPTEFFFHAIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVA
                     YDGTVRTTGGRIIQFKYGEDGTDPMKSSFGDPVDVKGIVESILKEEV"
BASE COUNT          833 a          916 c          842 g          947 t
ORIGIN      
        1 aatgggcttt cccggcagtg gccttgtcgg aagtattgcc ctccagtaca tggttgacca
       61 gcttgagttc acgcagatcg gaagcatgaa cagcaaatac ttcccccctc ttgcgatgat
      121 gactcgcggc gtgattaacg tgccggtaag ggtatatgaa aaagagaatc ttgcggcaat
      181 tgttgctgac atccccatcc acccgaatat atgttatgaa gtggcaaacg gtatcatgga
      241 atggctgatg ccattcaaaa tcaaggagat tgtcaccatt gcaggtatta tcaccaacga
      301 gatggaaaaa cgggttttcg gagtggctac ttccgagaag gcactgcaga gaatacagga
      361 tagcacaatc attctcccta tcgggagtat ctcgggtatt gcaagcagtt tcctcacgga
      421 atgcaggata cgaaatgtcg atgcatatgg tttacttggc gagacaatca atgcccccga
      481 tccgagagct tcagcggcta cgatagaagt tctgaacaag atgtacggtc ttacgctcga
      541 tattcagccc ttgcttgatc aagctgaaga gatcgagtca accatgcata aactcgcaga
      601 gcaggttcag acggctgaag caactcctaa aaaggagcag cagctcccga tgtacgggtg
      661 atccataatg aaatgttttg ctttaacagg gatttctccg gacgtgatac aggaactcca
      721 caaaggcaga ccgcgtaccc ttgaactcca aagtgcacat aatgttgtca ccctggcagg
      781 cctcgatccc ggtgcggccg tgttcatgac ttcggtggat atggaagacc ttgatccagg
      841 tgacacggga atcattgttg agattattgc gataagcatc acaatgaagc gaatgatgga
      901 gtttacacag ggatatcatt tcgaggagcg ggaaaggatg tcagcccgga taaaggtaag
      961 gtgcgtcgga tcttcaattg tcaaatctgt cactcatgaa ggaattatgc atccggtctc
     1021 tgtggatgtg gtgaaatcag cctgttacca tgccggctga cctggtatat ccccacaccc
     1081 tttctttttt ttatgtattc tgaaatatct atcctgtcgg gaacatacca tcacgaggca
     1141 atggttcttc aggcagcgat cgtatgaaac tacggtagag cgagaccatt ccgagattta
     1201 ataaggatag ataccaaaga gtatgggcaa ctcattgggc ttgtggctta gccaggatag
     1261 agcgccgggc ttctaacccg gatgtcgagg gttcgaatcc ctccaggccc gttctgacga
     1321 gtgtggtttg aaaaaggttg atcaggtttc ttcctcttct gtgacaagtg tcacatccga
     1381 gatattgtgc tggcggagcg ccagtttctt cgccttgaaa atatttttcc cttcctttcc
     1441 gatagcaata cctctgtctt cctgcctgac ctcgatatgg gctatccgtc cttcgtcctc
     1501 tgtctcaaat ccgactgtaa caacctgagc aggaaggaaa cagtttttga ggaattgctc
     1561 gggacttggc gagttctcaa caacctcgat ctttttcccc atcacttcca ttgctttctt
     1621 gatactggcc cctttctttc caatggcaag tcccatctca ccaggattga caacaaagat
     1681 cagcctgttg ttgcgatcat caatcacaca gtcccggctt ccagcaccgg tgagacgttc
     1741 aaactgggat atgaggcgca tgcagtcctc ggtcagaacg atctcggaca tctctcacgc
     1801 cctcttcaga ttgagaaggt cagattcacc gggattcact acagcaagtg cactgaccat
     1861 aaacggtttt ccgcacgcct tgccaagctg gacgcttgat ccctcgtacg tgtagatgaa
     1921 cagtccttcc cgtgaggata ataagttttt aaacgtttca gggcagttgc cggcaagaac
     1981 taccatctga gccttgcctt cgttaatgca ttgttcggtt tggttctgac cgaggataac
     2041 atccccggtc ttgatagctc ttcttaacga tacattaaaa tccattacaa ttcacctagt
     2101 tcaaagattt cgcgattagt tttacatctc cggtaccgag ctgaatgggc tggcccacaa
     2161 tcacgttctc agtaaccccg gacaattcat cgacctcatt tgcaacggcg gcatcgagca
     2221 ggtggttcac cgtgacctca aatgctgcac gtgacagcac actctctttc tcaccggcaa
     2281 ttccgtgacg gccgatctgt tttacttccc cttccatgca catcatgtca gcaacaagca
     2341 taatatggcg tacatcaaca aggataccct gttctcctaa cgtagagagc gcttcgtgaa
     2401 taattgcatt tctggcagcc tcgatcccga gtacctgcga gatctcgctg atgttgttgg
     2461 tgcgggtacg ggtggtatcc actccctcaa cctcaaacac gtctttaatg ttggagcctt
     2521 cagtatatag gatatactct ccgctctcct tccggacaac gacccgttcg atgtcatcga
     2581 taccctgcac gattacattt ctgacgtgtt cagcgagttg gaacagattc tggtaactct
     2641 ctctgtcctt gggcatgaaa atgatggtcg cggtcttctc atccatctcg aattcaaagt
     2701 cgcgatagtg tcttcgttcc ttgatcttct ttggagcaac ctcaaggata gataaggggg
     2761 cgatctttct cttttcacat accgccgtgt tgaggtggac aacgatctgc atgttttcca
     2821 tatcgatggt aatatccccg aactcgtgga gtggtgctgc ctcgatctgc cagctgactt
     2881 cccgtgcccg atccctgtca accccgaatt ctggttcgag gaagatggtc atcgtggggg
     2941 tgctcggctc ctttcttgcg tccatgatct cgatcagacg gggaaggccc agggttacgt
     3001 taatctcagc caccccggca tagtggaatg tccgcatcgt caactgggta ccgggctctc
     3061 cgatcgattg tgctgcgatg atgcctaccg cctcacatgc ttcgatccgg gtattctggt
     3121 actcattgac gacacggttc aggatctggt caaactgggt atcggtgatc tcttttcctt
     3181 cgagatactt tttaagctga tcttttgtct tatcggggag atctgcctca tcgatctttg
     3241 cttcatggtc agtctgcatc tcacacctcc tctttcagga tgctctcaac gatacccttt
     3301 acatcgaccg gatcaccgaa actgctcttc attggatccg ttccgtcctc tccatacttg
     3361 aactggatga tccttccccc ggttgtccgg acggtcccat cataggcaac cttgagatcc
     3421 tgcaaggcat tgatcatacg gcgctggagg tagccgctct gcgaggtacg gactgcggtg
     3481 tccaccagac cctcacgacc accgattgca tggaagaaga actcggtggg attcaggc
//
LOCUS       DAOOCO010000189         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_25817_length_3505_cov_4.984348, whole genome shotgun sequence.
ACCESSION   DAOOCO010000189 DAOOCO010000000
VERSION     DAOOCO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25817_length_3505_cov_4.984348"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PK154_09275"
     CDS             complement(<1..767)
                     /locus_tag="PK154_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPW11288.1"
                     /translation="MKTSYIDYAMSVIIGRAIPDVRDGLKPVHRRSLYAMWEMGNTSD
                     KPTKKSARVVGEVMGKFHPHGDASIYDTIVKMAQPFSYRHMLVHGQGNFGSIDGDSAA
                     ASRYTEVRLTPYAETLLEDIEKETVPFVPNYDESLEEPVVLPAKLPNLLVNGSDGIAV
                     GLATKMPPHNLREVCGAVRVYLDNPEAGMEEFIRVMPGPDFPTGGVLMGTEGARNVYA
                     TGQGRLVVRGVANIEEAASGSRGDRIIVTELPYQVNKA"
     gene            complement(832..2862)
                     /locus_tag="PK154_09280"
     CDS             complement(832..2862)
                     /locus_tag="PK154_09280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HPW11289.1"
                     /translation="MTETYDASHITVLEGLQPVRERPAMYIGSTDARGLHHLVYEVVD
                     NSIDEALAGYCTRIAVTINKDGSIAVEDNGRGIPVDRMEKNGKSALEVVLTVLHAGGK
                     FDKATYQVSGGLHGVGVSVVNALSSWLEARVYREGNIYEMRFAQGKVLSPPSQREETW
                     PELLTRYQAWYGEAAPLPAGKPAPGDQQGDLRREFLSRCGSRMTGTRITFIPDKTIFE
                     TTQFDYDLLSHRLRELAFLNAGLTIIITDQRTGDHVSYCYAGGLSEFVGYLNEGAACI
                     HPAPIYICRKDEENKVELEVAMQYTTGYDEKIFSYVNSVNTREGGTHLEGFRSAITRA
                     INIVAKRNGLIKENATITLRGEDVREGLTSVVSVKMASPQFEGQTKMRLGNSNVKGIA
                     DSLVYAALTEYFDENPKVLQAIVEKALSAAKAREAARNARELARRKSTLESAGLPGKL
                     ADCSERDPKKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKAGEHQILKNA
                     EIQTLISAIGTGIGEKFEIDHARYHHIVIMTDADVDGAHIRTLLLTFFYRYMLRLIEA
                     GYVYIAQPPLFSVLKGKEERYAYREEDMKRILSELGEKGVSVKRYKGLGEMNAQQLWD
                     TTMNPEQRIFKQVTIEDAMEANEIFKTLMGKDVEARKDFIKRHAKEVTNLDI"
     gene            complement(2957..3265)
                     /locus_tag="PK154_09285"
     CDS             complement(2957..3265)
                     /locus_tag="PK154_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11290.1"
                     /translation="MESKFGRGFITTIVLICRHFALPPEQAFYGAADHLDGFEIPPQY
                     KGTEVEELALKLRKRIVWHQPGTLDREEAAEVIRILNRLIIAIDTSLGITNPELGEFL
                     "
     gene            complement(3307..>3505)
                     /locus_tag="PK154_09290"
     CDS             complement(3307..>3505)
                     /locus_tag="PK154_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HPW11291.1"
                     /translation="TLFIRSYEQGERTYTAMLCRGYGRESHLYIAKKPLKGRDWTFLV
                     AGLLLIIATPLLAWTRIIVLA"
BASE COUNT          612 a         1079 c         1021 g          793 t
ORIGIN      
        1 gccttgttga cctggtaggg gagctcggtc acgatgatcc ggtccccgcg gctgccgctt
       61 gcggcctcct cgatattcgc aacaccccgg acgacgagcc ggccctggcc tgtcgcatag
      121 acgttcctgg ccccttcagt ccccatcagg actcctccgg tcgggaaatc ggggcccggc
      181 atgacccgta tgaattcctc catgccggct tccgggttgt cgaggtatac cctgaccgcc
      241 ccgcagacct cccgcaggtt gtggggaggc atcttggtgg caagcccgac cgcgatcccg
      301 tctgaaccgt tcacgagcag gtttggtaac ttagccggga gaacgaccgg ctcttcgagc
      361 gattcatcat agttcgggac gaaggggacg gtctctttct cgatatcctc aaggagtgtc
      421 tcggcgtaag gggtgagtct cacctcggtg taccggcttg ctgccgcaga atcaccatcg
      481 attgacccga agttgccctg gccgtggacg agcatgtggc ggtaggagaa gggctgggcc
      541 atcttgacga tcgtgtcata gatggacgca tcaccgtggg gatggaactt ccccatcacc
      601 tctcccacaa cacgggcgct cttcttcgtg ggcttgtcgc tggtattccc catctcccac
      661 attgcgtaga gcgatcggcg gtgaacgggt ttcagcccgt cacgcacatc ggggatggcc
      721 cgaccgatga tgacactcat cgcgtaatcg atataggagg tcttcatctc gtgctcgatg
      781 ctgaccggct cgacgcgggg ggtgggggta tcgtccggtt tacccgcctc ctcagatgtc
      841 aaggttcgtc acctcttttg catgccgctt gatgaaatcc ttccgggctt caacatcctt
      901 gcccatcagg gtcttgaaga tctcatttgc ctccatggcg tcctcaatcg tgacctgctt
      961 gaagatccgt tgctccgggt tcatggtcgt atcccagagc tgctgggcgt tcatctcccc
     1021 aagacccttg taccgcttga ccgagacccc cttctccccc agctcggaga ggatccgttt
     1081 catgtcctct tcgcggtagg cataccgctc ctccttcccc ttcaggacag agaagagcgg
     1141 gggctgggcg atgtagacat agccggcctc gatcagcctg agcatgtacc ggtagaagaa
     1201 ggtgaggagg agggtccgga tatgggcgcc gtcgacgtcg gcatcggtca tgatcacgat
     1261 atggtggtat cgtgcatggt cgatctcgaa cttctccccg atacccgtgc caatagcaga
     1321 gatgagcgtc tgtatctcgg catttttcag gatctggtgc tccccggcct tctccacgtt
     1381 caggatcttc ccccgcagcg ggaggatggc ctggaatttc cggtcccttc cctgcttggc
     1441 gctcccgcct gccgaatctc cttccacgat atagatctcg ctctttttcg ggtcacgctc
     1501 ggagcagtct gccagcttgc cgggcaggcc tgcactctca agcgtgctct tccgccgggc
     1561 aagctcacgg gcgttccttg cagcctcccg tgcttttgcc gcagacagcg ccttctccac
     1621 gatggcctgg agcaccttcg ggttctcatc gaaatattcg gtgagtgcgg cataaacgag
     1681 ggagtcggcg atccctttca cattgctgtt ccccagccgc atcttggtct gcccctcgaa
     1741 ctgggggctc gccatcttga ccgaaacgac cgaggtcagg ccttccctga catcctcgcc
     1801 ccgcagcgtg atcgtggcgt tctccttgat caggccgttc cgctttgcaa cgatattgat
     1861 ggcacgggtg atggcgctcc tgaatccctc caggtgggtc ccgccttccc gggtattgac
     1921 ggagttgacg tagctgaaga tcttctcatc gtatcccgtg gtatactgca tcgccacttc
     1981 gagctcgact ttattctcct cgtccttccg gcagatgtag atcggggccg ggtggatgca
     2041 tgccgctccc tcgttcaggt atcccacgaa ctccgacagg ccccctgcat agcagtagct
     2101 gacatggtcc ccggtccgct gatcggtgat gatgatggtg aggcccgcat tcaggaaggc
     2161 gagctcccgg agccgatggg agaggagatc gtaatcgaac tgggtcgtct caaagatcgt
     2221 cttgtcaggg atgaaggtga tccgggtacc cgtcatcctc gatccgcagc gggagaggaa
     2281 ctcccgcctc aggtcaccct gctggtcgcc gggagctggc ttgccggccg ggaggggtgc
     2341 cgcttcacca taccaggcct ggtaccgggt gagcagctcg ggccaggtct cctcgcgctg
     2401 cgaggggggc gagaggacct tcccctgggc aaaccgcatc tcgtagatgt ttccctcgcg
     2461 gtacacccgg gcctcaagcc agcttgacag ggcattgacg accgatactc ctaccccgtg
     2521 gaggccgccc gagacctggt aggtcgcctt gtcgaatttt ccccctgcat ggaggacggt
     2581 gaggaccact tcgagggcgg actttccgtt cttctccatc cggtcgacgg gaattcccct
     2641 cccgttgtcc tccaccgcaa ttgaaccgtc cttgttgatc gtcaccgcga tgcgggtgca
     2701 gtacccggca agggcttcat cgatcgagtt gtccaccact tcatacacga gatggtggag
     2761 ccctctcgca tcggtactcc cgatatacat ggccggtcgt tcccgtaccg gctgcaggcc
     2821 ttcaagtacg gtgatatgcg atgcgtcata cgtctcggtc aatcactcac ctccccgtga
     2881 aaaatgtatt gaaattcctg accgtcacac taagtattgg tcgttttccc tcttaatggt
     2941 tcagacggcc aggaattcag aggaattcgc ccagttcggg gttggtgatc ccaagcgatg
     3001 tgtcaatcgc aatgatcagc ctgttgagga tccggatcac ctcggcggcc tcttcccggt
     3061 caagggtgcc cggctggtgc cagacgatcc gtttcctcag cttcagcgcg agctcctcca
     3121 cctctgtccc cttgtactgc ggggggatct cgaacccgtc caggtggtcc gccgcgccgt
     3181 aaaacgcctg ttcgggaggc agggcaaaat gcctgcagat gagcacgatg gtcgtgataa
     3241 atccccgtcc gaatttactc tccatgaaag agatatcagg tccggggagg ataaaggatg
     3301 cgggggtcag gcaaggacga tgatccgggt ccatgcgagg aggggggtgg cgatgatcag
     3361 cagcaatcct gccacgagga atgtccagtc cctccctttc aggggttttt tcgcgatgta
     3421 caggtggctc tcccgtccgt atccacggca gagcatggca gtgtacgtcc gttccccctg
     3481 ctcgtaggac cggatgaaga gggtc
//
LOCUS       DAOOCO010000190         3498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_25906_length_3498_cov_5.117920, whole genome shotgun sequence.
ACCESSION   DAOOCO010000190 DAOOCO010000000
VERSION     DAOOCO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25906_length_3498_cov_5.117920"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PK154_09295"
     CDS             complement(<1..1390)
                     /locus_tag="PK154_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPW11292.1"
                     /translation="MKKRYLSLQTLSAALTIMKTAFPQSGGSVTISITESEGRVASEP
                     IYARYTVPDSPTAEMDGIAVKSSETVGAGEQRPISLSNFMRVDTGNIVPAAFDAVIMI
                     EDVRLDGSSCTIRKAARPGQHIRPPGQDVRKGDLIIPQGHQIRSSDISALASYGYDEV
                     AVKVVRVGIIPVGNELVQIGTIPRPGQVIETNSLFASSFLSRLGAICTTYDLVRDDPP
                     LIEETLQRAVSEQDLVLISSGTSAGRHDYSARTIMKLGRLLFHGVAMLPGKPAILGEI
                     GGKPVVGLPGYPVGSQTVLRELVAPFLVSWGLAPLAGWIVRGRLARDMPSELGFDEYV
                     PVTVGSVNGSSWIFPHPRGSGLQMNLVRANGYLHIPLASEGWESGAWVNIHCTSLPHQ
                     IDRTLLCVGAKNPGITQLAYALANRSVMLQSFYDLHSRALHSLKNNSCNMVALNIPGL
                     EFNPDMFDGLKDV"
     gene            complement(1774..2397)
                     /locus_tag="PK154_09300"
     CDS             complement(1774..2397)
                     /locus_tag="PK154_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPW11293.1"
                     /translation="MSRIENEIQSRMDSHAISPRLQDYFNRCIEFHTYPAPGLLIGVF
                     MVDYAVEQLGATPGEKLYSVCETQKCAPDPLQVLLHCTSGNHRLRVIPMGRFALTLNR
                     PSIEPFTEGIRVFIDPGKLERYPLINSWFTNDPSFNQREKALPLIDEIFRAGRDILSA
                     EKVRVKVTPKQKWQSGKCRICGEMVPSDLLEEGVCIGCGPNAYYKKA"
     gene            2628..>3498
                     /locus_tag="PK154_09305"
     CDS             2628..>3498
                     /locus_tag="PK154_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW11294.1"
                     /translation="MSNSKPETLKYVPTTCPYCGVGCGLNLIVKDGKAVGVAPWQRSP
                     INEGKLCPKGMTCWEFIHSPDRLTKPLIKKNGKFEEASWDEALDLVATKFKEISDKYG
                     PKSLGFQVSCRTPNEECYIMQKLARVAFKTNNVDNCARICHGPSVAGLSLSFGSGAAT
                     NPFEDILNSDFILVWGSNAVEAHPLAGRRIAQAKLKGIPIMVIDPRFSPTARLADTWV
                     RFNPSTHIALANSMMYWIIKENLHDKEFIEKRTKGFEDLKKTVENYADVEKITGVLPC
                     HLLYGLGRYPGNLL"
BASE COUNT          892 a          887 c          873 g          846 t
ORIGIN      
        1 agacgtcttt taatccatca aacatatccg gattgaactc cagacccggt atattcaatg
       61 caaccatgtt acaggaattg ttcttgaggg aatggagcgc ccgggaatgg agatcataaa
      121 aactctgcag catcactgaa cgattcgcca gggcataggc cagttgggtt ataccaggat
      181 tttttgcacc tacacagaga agggtgcggt cgatctggtg aggcagggag gtacaatgga
      241 tattcaccca tgccccggac tcccatcctt cacttgcaag agggatatgg agatatccat
      301 tggccctgac caggttcatc tggagacctg aaccacgggg atgaggaaag atccaggaag
      361 aaccgttgac tgaccctacc gtgacgggaa catactcatc aaatcctagt tccgacggca
      421 tgtcccgtgc aagccttcct ctcacgatcc atccggcaag aggggcaaga ccccatgaga
      481 cgaggaacgg ggccacgagt tcacggagga ccgtctggga gccgacagga tatcccggca
      541 atcctaccac cggttttccc ccgatctccc cgagtatcgc aggtttccct ggcagcatag
      601 caacgccatg gaagaggagc cttccaagtt tcatgatggt ccgggcggaa taatcatgcc
      661 tgcccgccga tgtccctgat gatatgagca caaggtcctg ttcggaaacg gccctttgca
      721 gtgtttcttc aatcagaggg ggatcatcac gtacaagatc ataagtggta cagatcgctc
      781 ccaggcggga aaggaatgag gaggcaaaga ggctattggt ctcgatcacc tgcccaggcc
      841 tgggtattgt accgatctga acaagctcgt tcccgacagg gataatcccg acacgtacca
      901 cctttaccgc gacctcatca tatccatacg aggccagggc gctgatatca gaagaccgga
      961 tctggtgacc ctgcgggatt atcagatcac ctttccttac atcctgtccg ggaggccgaa
     1021 tatgctgccc tggccgggct gcttttcgta tagtacagga agaaccatcc aggcgtacat
     1081 cttcaatcat gataaccgca tcaaaagcag cggggacaat gttcccggtg tcaaccctca
     1141 taaaattgga gagtgagatg gggcgctgct caccagcccc cacggtttcg ctgctcttca
     1201 ccgcaatacc atccatttct gcagtgggtg aatcgggcac ggtataccgg gcatagatcg
     1261 gctctgatgc aaccctccct tccgattcgg tgatggagat cgtcaccgat cctcctgact
     1321 gaggaaatgc cgtcttcata atagtgagcg cagcactcag ggtctggagg gaaaggtaac
     1381 gcttcttcat tctgttatac ctcaaaggag gattatggat tcacggacat gatacatcga
     1441 cccaggatat ccacgagaga gaggtaacta atatttactc aactaatatg taaattaatc
     1501 attattaaca tttaggagga accattgatt atcatatcgg attcggaagt tactcaagga
     1561 cactatcgga tggaatgaag atatcccggt atacggttat tctgttccat cgttcagctg
     1621 tttccaaacc tggcacccgt gaatcccata aggaaggctt tgatggatcg gccctgctat
     1681 tgcaggtgat ctcctcactt gtcagatcac ctcccaacaa tcaggtagct ccggccgctc
     1741 cattctgatt ggattatttc cgctcctgac gggtcatgct tttttgtagt acgcattcgg
     1801 gccgcacccg atgcagacac cctcttccaa caggtctgac gggaccatct cgccgcagat
     1861 acggcattta cccgattgcc atttctgctt gggagtcacc ttcacacgga ctttttcagc
     1921 ggagagtatg tcacggcctg ccctgaatat ctcatcgata aggggtaatg ctttttctct
     1981 ctgattgaac gaaggatcgt tggtaaacca ggaattgatg agtgggtacc tctccaattt
     2041 tcccggatct atgaataccc tgatgccttc ggtgaagggt tcaatcgagg gccggttcag
     2101 ggtcaatgcg aaccttccca tggggataac ccgtaaccga tgattcccgg acgtgcaatg
     2161 gaggagaacc tggaggggat caggggcgca tttctgagtt tcacagactg aatacaattt
     2221 ttctccggga gttgcaccga gttgctcgac tgcgtaatcg accatgaaca ccccgataag
     2281 gagacctggt gcaggatagg tatgaaattc aatgcagcga ttaaaataat cctgtaatcg
     2341 tggtgagatg gcgtgagagt ccatccgcga ttgaatttcg ttctcaattc ttgacatgat
     2401 aacctcatta tcttactatt attctcttat caatggtatg atgtgaacaa atttgcgcgt
     2461 ctctgaaaat cagtgaatga cgagggggat ggttaataaa ttatatttaa ttaaatcgcc
     2521 taattgttaa ttataattgt ttatatttta tataccttca agtcctgctt attctattgt
     2581 acaacaattc atcgatgacc ctaggaaaga tttccggaac gatttctatg agtaattcca
     2641 aaccagaaac cctgaaatac gtgcccacta cctgccccta ctgcggtgtg gggtgtgggc
     2701 tgaacctcat cgttaaggat ggtaaagcag ttggtgtcgc cccctggcag aggagtccga
     2761 tcaacgaagg gaaactctgc ccgaaaggta tgacctgctg ggagttcatc cacagccccg
     2821 atcggttgac aaaaccgctg atcaaaaaga acgggaagtt cgaagaggca agctgggacg
     2881 aagcccttga cctggtagca acgaaattca aagagatatc ggataaatat ggtccgaaat
     2941 cactcgggtt ccaggtttcc tgcaggacac cgaacgaaga atgctacatt atgcagaaac
     3001 ttgcccgcgt cgcattcaag accaacaatg tcgataactg cgcacgcatc tgccatggcc
     3061 cctcagtcgc cgggctctca ctctcgttcg ggtccggtgc ggccacgaat ccgtttgagg
     3121 acatcctgaa ctccgacttc atcctggtct ggggttcgaa cgccgtggaa gcacatccgc
     3181 tggcaggtag gcggattgcg caggcgaagc tgaagggtat tcctatcatg gtcatcgatc
     3241 ccaggttcag ccctaccgca cggctcgctg atacctgggt ccgcttcaat ccatcgaccc
     3301 atatcgctct tgccaactcc atgatgtatt ggatcatcaa ggagaacctc cacgataagg
     3361 aatttatcga gaagcggacc aagggatttg aggacctgaa gaagactgtg gagaactatg
     3421 ctgatgttga gaagattacc ggggtacttc cgtgccatct cctttacggt ctcggtaggt
     3481 accccggtaa tcttctca
//
LOCUS       DAOOCO010000191         3482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_26065_length_3482_cov_4.803910, whole genome shotgun sequence.
ACCESSION   DAOOCO010000191 DAOOCO010000000
VERSION     DAOOCO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26065_length_3482_cov_4.803910"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK154_09310"
     CDS             <1..211
                     /locus_tag="PK154_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11295.1"
                     /translation="DDLWAELDRSDREIIPITEPTTIPTTEPTTVPETTLIPTTAEAP
                     LSPFIPLGCLLLAGILVASRKKRGN"
     gene            278..1138
                     /locus_tag="PK154_09315"
     CDS             278..1138
                     /locus_tag="PK154_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HPW11296.1"
                     /translation="MTINPDEHVIEAIGRCRIIVRNGSVVEVGPPLIRECPLARKFAL
                     PVHPITPEAVGENIAHRIRKFGMCTPDRKVLMSGEYVGFGASELISYGLSTGVFDAAV
                     IACDGAGTVIATKPDLVQGIGGRMSGLVSTCPYPPVIAAIQENGGIVPFGEDARMDQV
                     KGTRIAYEAGYSKVAVTIARADDAPKIHSEFTDAFIIAVHTTGISDEEALLFADYADI
                     VTACASRAVREVAGTRALLQAGSSVPVFAMTQKAKELILRKVIETEGQFLVTGAKLPF
                     GSDTLPKPLV"
     gene            complement(1224..1802)
                     /locus_tag="PK154_09320"
     CDS             complement(1224..1802)
                     /locus_tag="PK154_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11297.1"
                     /translation="MKKNNNPSIIVLVMNHPLPHSFSRSPVEEVCCRCMRASGAEIIS
                     LIIPPPYPGWPHIREKIKDMVMGAGEISHINGCMLRYSDLIPVAGGKNLPGTEEIAHL
                     ISGRFHCSFDNTQNEILLIETTIPHTIGSVHSIHDGPGKPGWTLIFTMNTTRQARFGS
                     LHGVLNWFDDARAGIHEIFDLVVPEEIVQALR"
     gene            1824..2528
                     /locus_tag="PK154_09325"
     CDS             1824..2528
                     /locus_tag="PK154_09325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HPW11298.1"
                     /translation="MRIIDRPSVAILQHERIEPGGLFAYYVRECGRELVQIPLYETNE
                     VPPLHATHLIIMGGPMSVNDEREFPWLISEKQIIKEWVAKGRPVLGICLGAQLIASSF
                     GALVYPCKKELGWSSVNAIGDELFPELPGRFNVFQMHGETFDIQEPGMLVFRGEEVLN
                     QAICIGSALGLQFHTELTLEMIQDWISFLPSQKQESLITQSRQYLPESEKICRTIADR
                     FLTAPSHAFSWMRSAG"
     gene            complement(2669..3271)
                     /locus_tag="PK154_09330"
     CDS             complement(2669..3271)
                     /locus_tag="PK154_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11299.1"
                     /translation="MNRGILFLFLSIFLALGTSGCISDLPPSIQTSPQVTPGGDIPVP
                     PATAQTIEVPGLGTSNYSIPVLLTDLDWELARGCGWTAENLSQSASLFMDDCRIQQLI
                     RDGWEITGIGIDMNLIGSRCRRSTHPEASESCDWCLDSGPTQSLSFLGITTVYLAHLH
                     EKTVNHYIADRQGNTQVIQTKDSEIIMIRNGTVLYTFQHC"
BASE COUNT          851 a          903 c          921 g          807 t
ORIGIN      
        1 cgacgacctc tgggctgaac tcgaccgctc ggaccgcgag atcatcccga tcacggaacc
       61 caccaccatc ccgaccaccg agcccaccac ggtcccggaa acaaccctga taccgaccac
      121 ggccgaggcc ccgctgagcc cgttcattcc gctggggtgc cttctgctcg ctggaatact
      181 cgttgcttcc aggaaaaaac ggggtaattg atcaccagga aaccgataat taccctccct
      241 ttcaattagt atgcacaata tcaggtgact cttattcatg accataaacc ctgacgagca
      301 cgtaatcgag gcgataggaa gatgcaggat catcgtccgg aacggctcgg tcgtcgaagt
      361 aggaccccct cttatccgcg aatgcccgct cgcccgcaag tttgccctcc ccgttcaccc
      421 gatcacccca gaagcagtcg gagaaaacat tgcccaccgg atccggaaat tcggcatgtg
      481 caccccggac cggaaagtcc tgatgtctgg ggagtatgtc gggtttggcg cctccgaact
      541 gatcagctac ggattgtcaa ccggggtgtt cgatgcagca gtcatcgcct gtgatggtgc
      601 gggaaccgtg attgccacaa agccggatct cgtgcagggt atcgggggga ggatgtccgg
      661 gctcgtgagc acctgcccct accccccggt catagcagca atacaagaga acggggggat
      721 cgtccccttt ggggaggatg cccggatgga ccaggtaaaa gggacccgga ttgcctatga
      781 agccggatac tcgaaggttg ccgtcacgat tgcacgtgcc gatgatgccc caaagatcca
      841 ttcagagttc actgatgcat tcatcattgc cgtccacacc actgggatct ccgatgaaga
      901 agcactgctc ttcgcggact acgcggatat cgtgaccgct tgtgcttccc gtgcagtcag
      961 ggaggttgcc ggaacaagag ccctcctgca ggccggatca tctgtcccgg tctttgccat
     1021 gacccagaag gcgaaagagc tgatccttcg gaaggtcata gaaacagaag ggcagttcct
     1081 tgtaacaggt gcgaagctgc cgtttggatc agacacgctc ccgaagccgc tggtgtgacc
     1141 atgagaattg atctcggttc tcttttcatc atcaaaccaa gatactgtct ttccattaat
     1201 cccttaaaaa aattcaatga ggattacctg agcgcctgca cgatctcctc aggaacgaca
     1261 aggtcaaaga tttcgtggat cccagcccgg gcatcatcaa accagttcaa tacaccgtga
     1321 agggacccaa acctggcctg tctagtggtg ttcatggtga agatcagggt ccatccgggt
     1381 tttccagggc cgtcatgaat ggaatggact gaccctatag tgtgtgggat cgttgtctcg
     1441 atgagcagga tttcattctg tgtattatcg aaggaacaat gaaatctccc ggagatcagg
     1501 tgtgcgattt cttcggttcc gggaaggttt tttccgccag cgacaggtat caggtcgcta
     1561 tacctgagca tacacccatt gatgtgggag atctctcctg cccccatgac catatccttt
     1621 attttttccc tgatatgcgg ccagcccgga tagggcggag gaattatcag ggagatgatt
     1681 tcagcgcctg atgcacgcat acaacgacag cagacctcct cgacaggtga ccgggaaaag
     1741 gaatgtggta gcggatggtt catgacaaga acgattatac ttggattatt attcttcttc
     1801 acatgctgaa taattgttag gatgtgagga ttatagacag gccgagtgta gctattcttc
     1861 agcacgaacg cattgaacca ggcggtcttt ttgcgtatta tgtcagggaa tgcgggagag
     1921 aactggtaca gatcccgctt tacgagacaa atgaagttcc tccccttcac gctacccacc
     1981 tgatcatcat gggcggcccg atgagtgtga atgatgagcg ggagtttccc tggctgatat
     2041 cagagaagca gatcatcaag gagtgggtcg caaagggacg gccggttctt ggtatctgcc
     2101 tcggtgcaca attgatcgct tcctcctttg gtgctttggt gtatccctgc aaaaaagaac
     2161 tgggatggtc atcagtgaat gcgatcggag atgaattgtt tcctgaatta cccggcaggt
     2221 tcaatgtgtt ccagatgcat ggagagacgt ttgacattca ggaacccggt atgttggtat
     2281 tccgtggaga agaggtgctg aaccaggcta tctgtatcgg cagtgcgctt ggcttgcaat
     2341 tccacactga actcactctc gaaatgatac aagactggat ctccttcctg ccatctcaga
     2401 agcaggaatc cctcattaca caaagcaggc agtatcttcc ggaaagtgaa aaaatttgca
     2461 gaacaattgc agaccggttt ctgactgcac cctcccatgc tttttcatgg atgaggtccg
     2521 ctggctgaaa gagcgatcga attttgaata ccgggtcacc ggtgccatca tttttcgagc
     2581 aggatacaac gaagaggatt atactcttcc aattcagact ctggccatgg aatggaagta
     2641 tgatgcagcc cgatcgaatg acgcgctcct agcagtgctg gaatgtatac agaacagtac
     2701 cattgcggat catgatgatc tctgaatcct tcgtctgtat aacttgggta tttccctgcc
     2761 tgtcggcgat gtagtgattc accgtctttt cgtgcaaatg cgcgagatac accgtagtaa
     2821 taccgaggaa actgagggat tgtgtcggtc ccgaatcgag gcaccagtcg caggattcac
     2881 tcgcttccgg atgcgtcgat cggcggcacc ggctcccaat caggttcata tcaataccga
     2941 tcccggttat ctcccatccg tcacggatca actgctgaat ccggcaatca tccatgaaga
     3001 gcgatgcact ttgtgaaaga ttttccgctg tccacccgca cccccttgca agctcccaat
     3061 cgagatcggt aagcaggacg gggatcgaat aattcgaggt cccaaggccg gggacctcaa
     3121 tagtctgagc ggttgccggg ggaacgggga tatctcctcc aggggtaacc tgtggggagg
     3181 tctgtatcga gggagggaga tccgagatgc acccggaggt gccgagcgcc aggaaaattg
     3241 agagaaatag gaataggata cccctgttca tcgcaatccc aattactcgt gatatccctt
     3301 aatctttcta tagccgctat tgcaattcga aacggatgcc ttatcgtggg caggaaagaa
     3361 ccagatgatg ttcgaaaacg tgctattttt cctatttagc gagtaaaagg gagcactttt
     3421 taccaagtgc gagggaaggg atttgaaccc tagaactcct acgagacgga cccctcaagc
     3481 cc
//
LOCUS       DAOOCO010000192         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_26611_length_3430_cov_4.290963, whole genome shotgun sequence.
ACCESSION   DAOOCO010000192 DAOOCO010000000
VERSION     DAOOCO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26611_length_3430_cov_4.290963"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..271
                     /locus_tag="PK154_09335"
     CDS             101..271
                     /locus_tag="PK154_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11300.1"
                     /translation="MEGCRVGTGDPAGDEAPVMQGSAPGTGRCGIPTGGSPSPVVTGY
                     GNGWTGIAWMVK"
     gene            379..1656
                     /gene="thiC"
                     /locus_tag="PK154_09340"
     CDS             379..1656
                     /gene="thiC"
                     /locus_tag="PK154_09340"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPW11301.1"
                     /translation="MSLVDDARSGIVTEEMRTVARQEGVTEDFVRRGVAGGHITIPVS
                     PYRDVKICGIGEGLRTKVNASIGTSTDIVDIAMEVEKAKAAERAGADTLMELSTGGDF
                     VEIRKQVIANTTLSVGSVPLYQAFIEAAIKDGAVVHMKEDDLFRITAEQAKLGTNFMA
                     IHTGINWETVKRLRNQGRHGGLVSRGGAFMTAWMIHNEKENPLYSEFDYLVEIMKEHE
                     VTLSMGNGMRAGAVHDATDRAAVQELLINAELADKAHAEGVQVIVEGPGHIPLDEIEA
                     NVILQKRVTNRKPFYMLGPLVTDIAPGYDDRVAAIGAAMSSSYGADFICYVTPAEHLA
                     LPTPDEVYEGVMSSRIAAHVGDMIKLKKTREADLEMGHARRDLDWERQFQVAMNPERA
                     RAIRAERMPADTDGCTMCGDYCALKIVNRHFSF"
     gene            1824..2381
                     /locus_tag="PK154_09345"
     CDS             1824..2381
                     /locus_tag="PK154_09345"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPW11302.1"
                     /translation="MSKQALREQAKAIRCRLTPEEVASRSEQIEHHLRSVINGESPVL
                     VYVSKASEVDTRGLIDRLLASGTAVVVPIIERESRTLRLSYLRDPAVLVESTFSVPEP
                     IGSEIPARAEEIAVVVVPMIAFDRRGHRLGYGAGYYDRFLSCHPHLRKIGIAFSCLEV
                     PEIPGDENDVAMDLIVTEEGIIETR"
     gene            2460..>3430
                     /locus_tag="PK154_09350"
     CDS             2460..>3430
                     /locus_tag="PK154_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW11303.1"
                     /translation="MADKDSFEVVVKEAARDDAGRGIARLSIEVMRALSLVSGDVIEI
                     AGKKKATAIVWPGFAQDTGQNILRIDGTVRANAGTGVDERVRIRKVEVGMAKKVVVQP
                     TQPIRLVGGEQYLARVLRGRPVMEGQTVRVEVIGNPITLVIAKVTPKGIAVVTEETAI
                     ELKETPYTGEEGKKKEVTDVHYEDIGGLSRELEMVREMIELPLRHPELFERLGIEPPK
                     GVLLYGPPGTGKTLIAKAVANEVDAHFVTLSGPEIMSKYYGESEGKLREVFEDAQENA
                     PAIIFIDEIDSIAPKREDTKGEVERRVVAQLLSLMDGLKGRGQVVVIG"
BASE COUNT          722 a          960 c         1104 g          644 t
ORIGIN      
        1 caggccagtc cttggtgctc acgtacacaa aacccccata gttgacgttc aaccggatca
       61 ccgtctctat aatgaatgcg cagggagttt tattcaaact atggaaggct gccgggtggg
      121 tacgggcgat ccggccggcg atgaagcccc tgtcatgcag ggttccgcgc cagggaccgg
      181 gcggtgcgga atacccacag gaggaagtcc ctcccctgtg gtgaccgggt acggcaacgg
      241 ctggacggga atcgcttgga tggtaaaata aagtgttgta aacgcaaatc aactttactt
      301 gtggagaaga caagcaagat ttagtccgca tttgttaaaa gaaggcttta tgaggtcttt
      361 ttcacaaacg acttaggtat gagtctcgta gatgatgcca ggtccgggat cgttaccgaa
      421 gagatgcgga ccgtggcccg gcaggagggt gtgaccgagg actttgtcag gaggggggtt
      481 gccggcgggc atatcaccat ccccgtctcc ccctaccgtg acgtgaagat ctgcggtatc
      541 ggcgaagggc tgcggacgaa ggtgaatgcc tcgatcggca cctcgaccga cattgtcgat
      601 atcgcgatgg aggtggagaa ggcaaaggca gccgagcggg ccggtgccga taccctgatg
      661 gagctctcca ccgggggcga ctttgtcgag atacgcaagc aggtgatcgc caacaccacc
      721 ctctcggtgg ggagcgtccc gctgtaccag gccttcatcg aggctgccat caaggacggt
      781 gccgtggtcc acatgaagga ggatgacctc ttccggatca ccgccgagca ggcgaagctc
      841 gggaccaact tcatggccat ccataccggg atcaactggg agaccgtcaa acggctccgg
      901 aaccagggga ggcacggggg gctcgtctcg cggggcggtg cgttcatgac agcctggatg
      961 atccacaacg agaaggagaa cccgctctac tctgagttcg actacctggt cgagatcatg
     1021 aaggagcacg aggtcaccct ctcgatgggc aacgggatgc gtgccggtgc ggtacacgat
     1081 gcgaccgacc gtgcagcggt ccaggagctc ctgatcaatg ccgagctcgc cgataaagcc
     1141 catgcggagg gggtccaggt gatcgttgag ggtcccgggc atatcccgct tgacgagatc
     1201 gaggcgaacg tgatcctcca aaagagggtc acgaaccgga agcccttcta catgctcggg
     1261 ccgcttgtca ccgacatcgc accgggatac gatgaccggg tagccgcgat cggcgctgcc
     1321 atgtcctcct cttatggtgc tgacttcatc tgttacgtca cccctgctga gcaccttgcc
     1381 ctccccactc ccgatgaggt gtacgagggc gtgatgagct cccgcatcgc tgcccatgtt
     1441 ggcgacatga tcaagctgaa gaagacacgc gaagccgatc tcgagatggg gcatgcccgc
     1501 cgggatctgg actgggagcg gcagttccag gtggcgatga atccggagcg ggcgagagcc
     1561 atccgggccg agcggatgcc ggctgacacg gacggctgca cgatgtgcgg ggactactgt
     1621 gccctgaaga tcgtaaaccg gcacttctcc ttctgatccc ctccccgatt ttttatatga
     1681 aaatgcctcc ggcattttca tcttttatgc ttgtggccct caaaggaatc atgtaatttt
     1741 tttaagtacc tggtatccag actgacagga ccggggctga atccacatcc ttatctgatc
     1801 actttcacca tggtactaca cgaatgagca agcaggcgtt gcgggagcag gcaaaggcga
     1861 tccggtgccg ccttacccct gaagaggtcg cgagccgttc tgagcagatc gagcatcacc
     1921 tgaggagcgt gatcaacggg gagagcccgg tgctcgtgta cgtctcgaag gccagcgagg
     1981 tggatacccg ggggctgatc gaccggctgc ttgccagcgg gaccgcggtg gtcgtaccca
     2041 tcatcgaacg cgagtcccgg acactccgcc tctcctacct ccgcgatcct gccgtcctcg
     2101 tagagagcac cttttctgtg cccgagccga tcgggagcga gatccctgcc cgggccgaag
     2161 agatcgcggt cgtggtcgtt cccatgatcg ccttcgatcg gagggggcac cgactcgggt
     2221 acggtgccgg gtactacgac cggttccttt cctgccaccc ccacttaaga aagatcggga
     2281 tcgccttctc ctgcctggag gtgcccgaga tccctggcga tgaaaatgac gttgctatgg
     2341 accttattgt gacagaagag ggcattattg agacccgata agatttccct gctacagaaa
     2401 tatttatata taaatatgca ctaactttta gtaaactctg tagttttggg ttgaacgaaa
     2461 tggcagataa agattcattt gaagtcgttg tgaaggaagc ggccagggac gatgccggcc
     2521 gtggcatcgc ccggctctcc atcgaggtga tgcgtgccct ctccctggtc tcgggcgacg
     2581 taatcgagat cgcggggaag aagaaggcga ccgcgatcgt ctggccaggg tttgcccagg
     2641 acaccggcca gaacatcctc cggatcgacg ggaccgtccg tgccaatgcc ggcaccgggg
     2701 tggacgagcg ggtccggatc cggaaggtcg aggtcgggat ggcaaagaag gtggtggtcc
     2761 agccgaccca gccgatccgc ctggtcgggg gagaacagta cctcgcccgg gtcctccggg
     2821 gccgtccggt catggagggg cagacggtca gggtggaggt gataggaaac ccgatcacgc
     2881 tcgtgatcgc caaggtcacc ccgaaaggca ttgcggtggt caccgaggag accgcgatcg
     2941 agctgaagga gaccccgtac accggcgagg aggggaagaa gaaggaggtc acggatgtcc
     3001 attacgagga tatcgggggc ctctcccgcg agctcgagat ggtccgggag atgatcgagc
     3061 tcccgctccg gcaccctgag ctcttcgaac ggctcgggat cgagccgccc aagggggtgc
     3121 tcctttacgg tccgccgggc accggcaaga cgctgatcgc aaaggcggtg gcaaacgagg
     3181 tggacgccca ctttgtcacc ctttcaggtc ccgagatcat gagcaagtat tacggggaga
     3241 gcgaggggaa actccgggag gtcttcgagg acgcccagga gaacgccccg gcgatcatct
     3301 tcatcgatga gatcgactcg atcgccccca agcgggagga taccaagggt gaggtggagc
     3361 gccgggtcgt tgcccagctc ctctcgctca tggacggcct gaaggggcgg gggcaggtgg
     3421 tggtgatcgg
//
LOCUS       DAOOCO010000193         3379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_27148_length_3379_cov_4.846270, whole genome shotgun sequence.
ACCESSION   DAOOCO010000193 DAOOCO010000000
VERSION     DAOOCO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27148_length_3379_cov_4.846270"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1929
                     /gene="iorA"
                     /locus_tag="PK154_09355"
     CDS             160..1929
                     /gene="iorA"
                     /locus_tag="PK154_09355"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPW11304.1"
                     /translation="MDQRYLLGNEAIALACLESPVGFVSGYPGTPSSEVIEILAAHPD
                     RSSYVEWSVNEKVAFENALAAAWCGVRSLVTMKHVGLNVAADPLMTSAYTGVTGGFVI
                     MSADDPFAHSSQNEQDSRMYAKFAKIPCLDPSTLQEAHDMIRDAFALSEALGLPVLFR
                     PTTRICHSKSNVTVGPVLPYSGKGEFHKDPRQYVVIPAHTRILHRKLNEKQPAVRKRI
                     LESGYNQYHFRGKRAIIASGIAAAYVQEIIPPDVSFMKIGAYPIDEEWLAGFVRKHQE
                     VLVVEELFPVVEEVVREVAGTVTILGKKSGHAPYEGELSPESVARIMESCGFSPAVHY
                     SSVQPAPDLPPRPPILCAGCMHRPVFYAIKKVFRDGIYPSDIGCYTLGLQLGAVDTTI
                     CMGASITVGSGFSHAGEERDIVATIGDSTFLHTGIPGLLNAVYNGANMTLVILDNRTT
                     AMTGHQPNPNTGCTACGGLSPQISLEALCRACGVAHVEMVDPYDLVMLTAALKTAKSL
                     PGVKVVIAKQPCVITARRAGVRRGKYRVDPDTCTGCGTCIKFGCPAIEQQDEKAWIND
                     LCSGCAVCAQICPAAAIGREVSR"
     gene            1926..2510
                     /locus_tag="PK154_09360"
     CDS             1926..2510
                     /locus_tag="PK154_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPW11305.1"
                     /translation="MKGSYDIMIVGIGGQGTILASNIIGEACILEGRTVRGAETHGMA
                     QRGGSVESHVRIDGKFGPLIAPGTADLLIGFDLLEGLRYSHFLRPDGKIVLSERLVIP
                     TSVYVQHLAIPSSEELVKQLSRFDLTLIKAEEIAKEAGSVLASNVVMLGAASHQIPLD
                     ESSLIQAVQRLVPRKTVEINTRAFTMGRKVGPGC"
     gene            2531..3334
                     /locus_tag="PK154_09365"
     CDS             2531..3334
                     /locus_tag="PK154_09365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW11306.1"
                     /translation="MEKSPLGPFELDTLYHGDALDLLPLIPGGSIDLIVTDPPFAIDF
                     RAQRLNYNRKGANVLEGYAEIAPDEYGSFTSRWLREAFRVLSDSGSIYLFSGWNRLRD
                     VLCGIDSAGFHTINHLIWKYQFGVFTRKKYVTSHYHILFAVKDPHDYTFNKMEHYPED
                     VWVINREYWKGKKKTPTKLPGEIVRKILFYSSNPGDIVLDPFLGSGTVAAVCRENGRH
                     YLGFELVEDYYRFASERISNDESLTMRKKCQGMPGKKKRRDHENAEGEI"
BASE COUNT          810 a          845 c          940 g          784 t
ORIGIN      
        1 ccggttatca gccgggcgca ccaccgggct atgctacggg cccataaatt gcctaaatag
       61 ttaaacaaca cgacttctta taggttttga aacaacggcc gaactcctat ataagggtgt
      121 gaaccagaat atctcataaa gaggacatct tggtggacta tggaccagcg ctatctcttg
      181 ggaaatgaag caattgccct cgcatgtctc gaatctccag ttggattcgt gagcgggtat
      241 ccgggaactc cttcttcaga agtgatcgag atcctcgcag cgcatcccga ccggagcagc
      301 tatgtggaat ggtcggtgaa tgaaaaggtc gcattcgaaa atgcactcgc agcggcttgg
      361 tgcggggtgc ggtctctggt aacgatgaag catgtgggac tgaatgtcgc agcagatccg
      421 ctgatgacaa gcgcatacac cggggtcacc ggagggtttg tcatcatgag tgcggatgat
      481 ccctttgcgc attcatcgca gaatgagcag gacagccgga tgtatgcaaa atttgcgaag
      541 attccatgcc ttgatccctc gacgttacaa gaggctcatg acatgatccg ggatgctttt
      601 gccctttccg aagcgttggg gctccctgtt ctgtttcggc cgacgacgag gatatgtcat
      661 tcaaagagca atgtcaccgt gggtcctgtt ctgccctatt ccgggaaggg ggagttccac
      721 aaagatcccc gacagtatgt tgtaattcct gcccatacaa gaatcctcca caggaagctc
      781 aatgaaaaac agccggcagt cagaaaacgg atcctcgaat ccgggtataa tcagtaccac
      841 ttccggggaa agcgtgcgat catcgcaagc ggaattgccg ccgcctatgt ccaggaaatt
      901 ataccgcctg atgtctcatt catgaagatc ggtgcttacc ctatcgatga agaatggctt
      961 gccggttttg tccggaagca tcaggaggtc ctcgttgttg aggagctctt cccggtcgtg
     1021 gaagaagtgg tgcgcgaagt agcaggtact gtgaccatcc ttggtaaaaa gtccggtcat
     1081 gcaccatatg aaggagaact ttcacctgaa agcgtcgcac ggattatgga gtcctgtgga
     1141 ttttcccctg cggttcatta ctcttcagtc caaccagccc ctgacctgcc cccgcggcca
     1201 cccatcctct gtgccggatg tatgcacagg cccgtcttct atgccattaa aaaggtcttc
     1261 cgagatggaa tttacccaag cgatatcggc tgctataccc tggggctcca gctgggagcc
     1321 gtggacacga ccatatgcat gggtgcctct atcacggtag gaagtggatt ttcccatgca
     1381 ggggaagaga gggatattgt ggctaccatc ggagactcca ccttccttca caccggcatt
     1441 cccggcctcc ttaatgcggt ctataacggg gcaaacatga ctctcgtaat ccttgacaac
     1501 cggacaacgg cgatgacggg ccatcagcca aatcccaata caggatgcac tgcctgtggg
     1561 gggctttccc cacagatctc acttgaagcg ttatgccgtg cctgtggggt tgctcacgtt
     1621 gagatggttg acccttatga tctggtaatg ctgactgccg ccttgaagac cgcaaaatca
     1681 ctccctggtg tcaaggtggt gattgcaaaa cagccgtgcg ttatcacggc caggagagca
     1741 ggggtaagga gaggaaagta cagggtcgat cctgacacat gcactgggtg cggcacctgc
     1801 atcaaattcg gctgccctgc tattgaacag caggatgaga aagcctggat caatgacctc
     1861 tgcagcgggt gtgcagtctg tgcgcagata tgccccgccg ctgctatcgg aagggaggtg
     1921 agcagatgaa gggttcatat gatattatga ttgtcggtat cgggggccag gggacgatcc
     1981 tcgcctcgaa catcataggt gaggcatgca tcctggaggg gaggactgtc cggggtgccg
     2041 agacccatgg catggcacag cggggtggat ctgtcgagag tcatgtcagg atcgatggta
     2101 aattcggccc tcttattgct ccaggaactg ccgatctcct tattggattt gatctgctcg
     2161 aaggactccg gtactcccac tttctgagac ccgatggaaa gatagtgctc tctgaaaggt
     2221 tggtaatccc aacttcggtg tatgtccagc atcttgcgat cccctcatct gaggagttgg
     2281 taaagcagct ctcgcgtttt gacctcaccc tgatcaaggc ggaagagata gcaaaagagg
     2341 ccgggagtgt gcttgcatcg aatgttgtca tgcttggtgc cgcatcccac cagattcctc
     2401 tcgatgaatc ttccctcata caggcggttc agcgtctcgt cccaaggaag accgttgaga
     2461 tcaatacacg ggcttttacc atgggccgga aagtgggacc cgggtgctga gcgacgagga
     2521 gaggcgcaga gtggaaaaat cgccgcttgg tccatttgag ctggataccc tgtaccacgg
     2581 agatgcgctg gatcttctcc ctctcatccc cggagggagc atcgatctca tcgttactga
     2641 tcccccgttt gctattgact tccgggcgca gagactgaac tataaccgca aaggggcaaa
     2701 tgtccttgag ggatatgcag agattgctcc cgatgagtac gggagtttca cctcccggtg
     2761 gctcagggaa gcgttccggg tcctctccga ttcagggagt atctacctct tttcgggctg
     2821 gaaccggctc agggacgtgc tgtgcgggat agactctgcc ggatttcaca ccatcaacca
     2881 tcttatctgg aaataccagt tcggggtatt tacccggaag aaatacgtca cgagtcacta
     2941 tcatattctc tttgcggtaa aagaccctca tgattatacc ttcaataaaa tggagcacta
     3001 tccggaggat gtctgggtca tcaaccgcga gtattggaaa ggaaagaaga agacgcctac
     3061 aaagcttcct ggagaaattg tcagaaaaat tctcttttat tccagcaatc ccggagatat
     3121 tgttctggat ccatttctgg gatcgggaac tgttgctgcg gtctgcagag agaatggcag
     3181 gcattatctt gggtttgagc tggtggaaga ctattaccgg tttgcatctg aacggatcag
     3241 caacgatgaa tcattaacga tgagaaaaaa atgccaggga atgccgggaa aaaagaaaag
     3301 aagggatcat gagaacgctg agggggagat ttgaactccc gaggggctgg acgccccaca
     3361 ggctttccag gcctgcgcc
//
LOCUS       DAOOCO010000194         3368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_27278_length_3368_cov_5.508301, whole genome shotgun sequence.
ACCESSION   DAOOCO010000194 DAOOCO010000000
VERSION     DAOOCO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27278_length_3368_cov_5.508301"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK154_09370"
     CDS             <1..218
                     /locus_tag="PK154_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HPW11307.1"
                     /translation="DHYQISFGALRLLDVTVGKDGKTLLVHTESDKDAPDAVILDTNR
                     RFRKYLDLVTGFSTKERVKKSKSIEEA"
     gene            complement(224..2989)
                     /gene="leuS"
                     /locus_tag="PK154_09375"
     CDS             complement(224..2989)
                     /gene="leuS"
                     /locus_tag="PK154_09375"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPW11308.1"
                     /translation="MRQIECDVLRLWEHAFEPNPSDAQKFYLNVAYPYPSGAMHVGHG
                     RTYIAPDVIARFWRMRGRTVLYPMAFHVTGAPVIGISKRIARGDQKAISLYRDLYKVP
                     PDVLAAFTNPLAIVRHFSDEYQRVMSACGISIDWRRRFTTVDPQYSRFIEWQWKHLGE
                     EGHVVKGAHPVRYCPQCDNPVGDHDLLEGDKAEILKFTLVMFHWNDVLIPTATLRPET
                     IYGVTNLWINPDVTYVKARVDGALWIMSRAAAQKISLQDHTVEICDEIPGSSLIDHVV
                     SHPLCGEIPILPARFVDPDMASGIVMSVPAHAPFDYIALRDLQADGRYTAISPVPLIT
                     VPGYGTLPAKDAVERAGILDQDDPRMDTLTQEVYSAEFGSGRLYERYGGEPVKIARDT
                     VSAQMMEQFGSIPMYEFDTRSVVCRCGGQVLVKILHDQWFLQYSDPAWKSQVSNLLSQ
                     MSLVPPEVRTEFERTIDWLKDWACTRKVGLGTRLPWDPAWLIEPLSDSTVYMAYYTIA
                     HHLKSFSPDLLTPEVFDYVFLGRESSALPEREKLDVMRKEFLFWYPYDFRFSAKDLIS
                     NHLTFQLFHHVAIFPKPLLPKGMVVFGMGLLNGAKMSSSKGNVFLLEDALKEFGADTV
                     RMFLVGSAEPWQDFDWRNELVASAKRQIERFMITVEESSTATGAPDELDRWLASRLQG
                     HITRMTEALERFQTRQALQEAFFGIESDLKWYRRRLPEGSAGSSYLAEFCSAWVRLLA
                     PIIPFSSEYLWKKLGRPGMVSFSDWPASDQAAVDKRLELAEDLLFRTVEDIESIVKII
                     QMSPSAITIYTSPAWKYRVFQAVARAADKGAVMKDLMKDEEMRKRGREVTDTVKQCTT
                     LIHRLPPDIVSQLLEEIPDETATFRKAEKFLRKEFGVPVIVRDAGESVHAKASLALPF
                     KPALVIE"
     gene            complement(3059..>3368)
                     /locus_tag="PK154_09380"
     CDS             complement(3059..>3368)
                     /locus_tag="PK154_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW11309.1"
                     /translation="NKVGIGIDIRPRSELEKQPAPPQAEEELQLGGGVKIRMDKKQLA
                     IICPDQAGKIVDVFAGKEYLFTATVNDTGEIHLAKNSSIAQEMIKRYNEGDTIRLRPV
                     "
BASE COUNT          828 a          867 c          818 g          855 t
ORIGIN      
        1 gagaccatta tcagatcagt ttcggggcac tccgactcct ggatgtaacc gtcggcaaag
       61 atggaaagac tcttttagtc cacaccgaat ctgacaagga cgcaccggat gcggtgatcc
      121 ttgatacgaa caggcgcttt agaaagtacc ttgatctggt aactggcttt tcaacgaagg
      181 aacgcgtgaa gaaatcgaaa agtattgagg aagcctgatc cctttactca atgacgagtg
      241 ccggtttgaa agggagagcg agagatgctt tcgcatggac gctctcaccg gcatcacgga
      301 caattactgg aacaccgaac tctttcctga ggaatttctc ggctttgcgg aaggtggccg
      361 tttcatcagg aatctcctca agcagctgcg aaacgatatc ggggggaagc cgatggatga
      421 gggttgtaca ctgcttcact gtatcggtta cttctctccc tctttttctc atctcttcgt
      481 ctttcattag atccttcatc accgcaccct tatccgctgc gcgtgcaaca gcctgaaata
      541 ccctgtattt ccatgcgggg gacgtgtaga tggtgatcgc ggaaggggac atctgaataa
      601 tcttcacaat tgattcgata tcctcaacgg tcctgaagag cagatcttct gcaagttcca
      661 gccttttatc cactgctgcc tgatctgagg ccggccaatc tgaaaatgaa accatgccgg
      721 ggcgaccaag tttcttccat agatattcac ttgaaaatgg aattattggt gcaagcagtc
      781 gtacccaggc agagcagaat tcagcaagat agctgctccc agccgaccct tccggaagtc
      841 ttctcctgta ccatttgaga tcggattcaa ttccaaaaaa agcctcctga agggcctggc
      901 gggtctggaa tcgttccagg gcttctgtca ttcgggtgat atgaccctgt agcctgctcg
      961 caagccatcg gtcgagttca tctggcgctc cagttgcggt agaggattcc tctacggtaa
     1021 tcatgaatcg ctcgatctgc cgctttgccg aggcaacaag ttcatttctc cagtcaaaat
     1081 cctgccatgg ttcggcgctc cccacaagga acatccggac cgtgtcggct ccaaactctt
     1141 tgagtgcatc ttcaagaagg aagacgttcc cttttgaaga tgacatcttt gccccgttca
     1201 ggagccccat gccgaaaacc accatccctt tcgggagaag aggctttgga aagattgcga
     1261 catggtggaa taactgaaat gtcaggtgat ttgagatgag gtcctttgct gagaatctga
     1321 aatcgtaagg ataccagaat aaaaattcct tcctcatgac atccagtttt tcacgctcgg
     1381 gaagagcaga gctctcccgt ccgagaaata cataatcaaa aacttccggg gtgagaaggt
     1441 caggggagaa tgatttcagg tggtgagcga tggtataata tgccatgtaa acggttgaat
     1501 cagaaagcgg ctctatcaac caggccgggt cccaggggag tcgtgttcca agccccactt
     1561 tccttgtgca ggcccaatcc ttaagccagt cgatagtcct ctcgaactcg gtcctcactt
     1621 caggagggac gagggacatc tgtgaaagaa ggttcgagac ctgcgatttc caggcaggat
     1681 cactgtactg gagaaaccat tggtcgtgaa gaatttttac aagcacctga ccgccacacc
     1741 tgcagactac cgaacgggtg tcgaattcat acattggtat ggaaccaaac tgctccatca
     1801 tttgggctga aacagtatct cgtgcaattt tgacgggttc ccctccatac cgttcatata
     1861 accggccgga accaaattct gcactatata cctcctgggt gagagtgtcc atcctgggat
     1921 catcctgatc gagtattcct gcccgttcaa ctgcatcctt tgcagggagt gttccatagc
     1981 ccgggaccgt aatgagcggc acgggagaaa ttgcggtata tctgccgtct gcctgtaagt
     2041 cacgaagtgc gatataatcg aatggagcat gagcagggac actcatcaca atgccactcg
     2101 ccatatcagg atccacgaac cgggcgggaa ggatcggaat ttcaccacag agtgggtgcg
     2161 ataccacgtg atcaatcaag gaactgccgg ggatctcatc acaaatttca acggtatggt
     2221 cctggagtga tatcttttgg gcagctgccc tgctcatgat ccatagtgcc ccatccacac
     2281 gagccttcac atacgtaaca tccggattga tccacagatt ggttactccg tagattgttt
     2341 caggcctgag ggttgctgtt ggaatcagga catcattcca gtggaacata acaagggtga
     2401 atttaaggat ctctgcttta tccccttcga gaagatcatg gtctccgacc ggattgtcac
     2461 actgcggaca atatctcacc ggatgtgcac cctttacgac atggccttcc tctccaaggt
     2521 gtttccattg ccattcgatg aaccggctgt attgggggtc taccgtcgta aaccgccttc
     2581 tccagtctat tgagatgcca catgcgctca tgacccgctg gtattcgtca gaaaaatgcc
     2641 tgacgatagc cagaggattg gtaaatgctg caagcacatc agggggaact ttatagagat
     2701 cgcggtatag ggatatggct ttttgatctc ctcttgcaat acgcttggaa attccaatca
     2761 ccggagcccc ggtcacatgg aaagccatgg ggtaaaggac ggtcctcccc cgcatccgcc
     2821 agaaacgggc gatcacatca ggtgcgatgt aggttcttcc atgccccacg tgcatcgcac
     2881 cactagggta ggggtatgcc acattcagat aaaatttctg tgcatcgctg ggattcggtt
     2941 caaaagcgtg ttcccataat ctgaggacat cacactcaat ctgccgcata tcaggttcgc
     3001 tcacgcattc accatccttg aaatatcatc tcactatcca aagatattga aaaattagtt
     3061 ataccggtcg taaccggatg gtatcccctt cattataccg cttgatcatt tcctgggcga
     3121 tactcgaatt ttttgcaagg tggatctctc cggtatcatt cactgttgca gtgaagagat
     3181 attctttccc ggcaaagaca tcgactatct ttcccgcctg atcagggcag atgatggcaa
     3241 gttgcttctt atccatgcga attttcactc cccctcccag ctggagttcc tcttctgcct
     3301 ggggaggtgc cggttgtttt tcaagttcgc tccttgggcg gatatcgatg ccaatcccta
     3361 ccttgttc
//
LOCUS       DAOOCO010000195         3303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_28040_length_3303_cov_3.921798, whole genome shotgun sequence.
ACCESSION   DAOOCO010000195 DAOOCO010000000
VERSION     DAOOCO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28040_length_3303_cov_3.921798"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="prs"
                     /locus_tag="PK154_09385"
     CDS             complement(<1..599)
                     /gene="prs"
                     /locus_tag="PK154_09385"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPW11310.1"
                     /translation="MKVISTEYSQILAARLANAMKSDLVDVRFSRFPDGELNLKAGQV
                     GDEVLVVGSVTCSDALVQLVLLIDACQGSEITLVVPYMGYARQDKQFSPGEPLSARAI
                     AGVLSREIQRAFLVNVHDPGVLRFFSVPAQTLSLAPEIGTFLKQRGCMHPLILAPDDG
                     AARFAAEVAGVNGWEFDFLEKTRISSETVSMSPKNLVVKG"
     gene            683..2599
                     /gene="lonB"
                     /locus_tag="PK154_09390"
     CDS             683..2599
                     /gene="lonB"
                     /locus_tag="PK154_09390"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HPW11311.1"
                     /translation="MESTESGHETQEEPLSFDISEESSSQIEVPKKLIDQVIGQEHAV
                     EVIRKAATQRRHVMMIGSPGTGKSMLAKAMAELLPKEELQDILVYPNSDDPNNPIVRT
                     VPAARGKQIVTAHKAEARKKIQFRNTLVMLLILGIVGYAFITYQWLMGIIAAAFIFMA
                     MRYATPREETMVPKLLVTNDATVDAPFIDGTGSHAGALLGDVRHDPFQSGGLETPSHD
                     RVEAGAIHRAHKGVLFIDEINTLTPHSQQNLLTALQEGEFPITGQSERSSGAMVRTEP
                     VPCRFIMVAAGNLDAIQGMHPALRSRIRGYGYEVYMSESMPDTPENREKFIRFIAQEI
                     KNDGKIPHFDKGAMEEILREARRRSNRKGHLTLKLRDMGGLIRVAGDLARQEGVEVTT
                     ADHVIAAKKTARSIEDQVSDEYIGRSREYELTVVEGTRVGRVNGLAVMGSDSGSVLPI
                     MAEVTMAQGANGTVIATGMLKEIAQESIKNVSAILKKFTGKDIKNMDIHIQFIGTYGG
                     VEGDSASISVATAVISAIEGIPVRQDVAMTGSLSVRGDVLPVGGVTYKIEAAAKAGIT
                     HVLIPSANLDDVLIEERYRPMVTVIPVDNIEDVLKIALVPENRDGFLQKLRRMAISTS
                     EKLIEGAGFSPSIV"
     gene            2605..>3303
                     /locus_tag="PK154_09395"
     CDS             2605..>3303
                     /locus_tag="PK154_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HPW11312.1"
                     /translation="MDGIRYYDIRHVCGQSTHIEIDNGVIESAGTSFFDHAVIRALSA
                     KGWGILTVDNFGGTPEKERARLIRDAARISAITAQEVSLADATRGIRGIPPMHEDPRD
                     VSIGEKAEIMLSIERAARIEEIVNTRANYTERFEEVTFHDSSGASEQYEICRSGFGIM
                     AVASRAGSLQMGYERRHSIRGLNIRHQQDLGRSAAERAVALLSARPARGGTMNAVLDP
                     ELAGVFAHEAVGHAS"
BASE COUNT          766 a          957 c          970 g          610 t
ORIGIN      
        1 ccttttacaa cgagattctt gggggacatg ctcaccgttt cagaggagat tcgggtcttt
       61 tcgaggaagt cgaactccca cccgttcacc ccggcaactt ctgcagcaaa acgggcggca
      121 ccgtcatcgg gcgcaaggat gagagggtgc atacatcctc tctgtttcag gaatgtcccg
      181 atttcgggtg caagggagag ggtctgcgca gggacgctga aaaaccggag gacgccggga
      241 tcatggacat tgacaagaaa tgccctctgg atctccctgg agaggacccc ggcaatagcc
      301 cgggcagaga gaggttcccc gggcgagaac tgcttgtcct ggcgggcgta tcccatgtag
      361 ggcacaacca gggtgatttc ggacccctgg catgcgtcga taaggaggac gagttggacg
      421 agggcatcgc tgcaggtgac actcccaacc accagcactt catcgccgac ctggcctgcc
      481 ttcagattca gttctccatc cgggaaccgg gagaaccgca catccaccag atcggatttc
      541 atcgcatttg caagccttgc tgccaggatt tgtgagtatt ctgtgcttat taccttcatc
      601 tcatcctacc tcatgctgaa agtacttaaa ctaccatgga taagttatag tatcgtatta
      661 tttttacgag gtcaatctgc cgatggaatc aacagaatcc ggtcatgaga cccaggagga
      721 gccgctttcc tttgacatca gtgaggagag ctcctcccag atcgaagtac ccaaaaaact
      781 catcgaccag gtcatcggtc aggagcatgc agtcgaggtc atcaggaagg ctgccaccca
      841 gagacggcac gtgatgatga taggcagccc cggtaccggc aagtccatgc tcgccaaggc
      901 gatggccgaa ctgctcccga aagaagaact gcaggacatc ctcgtatacc cgaattcgga
      961 tgatccgaac aacccgattg tacggaccgt ccctgcggcg agaggcaagc agatcgtcac
     1021 tgcccataag gcggaagcaa ggaagaagat ccagttcaga aacaccctcg ttatgctcct
     1081 gatcctcggg attgtcggat atgcattcat cacctaccag tggctcatgg gtatcatcgc
     1141 tgcagcgttc atattcatgg cgatgaggta tgcaaccccc cgggaggaga ccatggtgcc
     1201 gaagctcctg gtcaccaacg atgcaacagt cgatgccccc tttattgacg gcaccggttc
     1261 ccatgcaggg gcgcttctcg gcgatgtccg ccacgatccc ttccagagcg gaggcctgga
     1321 aacccccagc catgaccggg tcgaagcagg tgccatccac cgggcccaca agggcgtcct
     1381 cttcatcgat gagatcaaca ccctcactcc ccactcccag cagaacctcc tcaccgccct
     1441 ccaggagggt gagttcccca tcaccggcca gagcgagcgt tcgagcgggg cgatggtcag
     1501 gaccgaaccg gtaccctgcc ggttcatcat ggtcgcggca ggaaacctgg atgccatcca
     1561 ggggatgcac ccggcgctcc gttcccgtat cagggggtac gggtacgagg tctacatgag
     1621 cgagagcatg ccggataccc ctgaaaaccg ggagaagttc atcaggttca tcgcccagga
     1681 gatcaagaac gacgggaaga tcccccactt cgacaagggc gccatggaag agatcctgcg
     1741 ggaggcgcgg aggaggtcca accggaaagg ccatctcacc ctcaaactcc gggacatggg
     1801 aggactgatc cgtgtcgcag gggatcttgc ccggcaggaa ggggtcgagg tcaccaccgc
     1861 tgaccatgtg attgccgcga aaaagaccgc ccgttccatc gaggaccagg tctcggacga
     1921 atacatcggc cggagccgcg aatatgaact caccgttgtc gaagggacac gggtcggaag
     1981 ggtgaatggt cttgcggtca tgggaagcga ctcgggctcg gtactcccga tcatggctga
     2041 agtcaccatg gcacaggggg caaacggaac ggtgatcgca accgggatgc tcaaggagat
     2101 cgcacaggag tccatcaaaa acgtgagtgc gatcctgaag aagttcaccg ggaaggacat
     2161 caagaacatg gatatccaca tccagttcat cgggacctat ggtggggtcg agggagattc
     2221 agcctcgatc agtgtggcaa ccgccgtcat cagcgccatc gaagggatcc ccgtacggca
     2281 ggatgtggcg atgaccggtt ccctttcggt caggggggat gtcctccccg ttggtggcgt
     2341 tacctacaag atcgaggcag cagccaaggc cggcatcacc catgtcctga tcccctcggc
     2401 aaatcttgac gatgtcctca tcgaggagcg ataccgtccg atggtcaccg tgatcccggt
     2461 tgacaatatc gaggacgtcc tcaagatcgc cctcgttccc gagaacaggg atgggttcct
     2521 gcagaaactc cgcaggatgg cgatatccac ctcggaaaag ctcatcgaag gcgccggatt
     2581 ctccccctca attgtctgat ctgaatggac ggaatcaggt attacgatat ccgccatgtc
     2641 tgcgggcagt caacccacat cgagatcgac aacggggtga tcgaatctgc cggcacctct
     2701 ttttttgatc atgcggtgat cagggcactc tccgcaaagg gctggggtat cctcaccgta
     2761 gataatttcg ggggaacccc ggaaaaggag agagcgcgcc tgatcaggga tgcagcccga
     2821 atctccgcga ttaccgccca agaggtctcc cttgccgatg caacgagggg gatacgcggg
     2881 atccctccca tgcatgaaga tcctcgggac gtaagtatcg gggagaaagc agagatcatg
     2941 ctctcgatcg aacgggcggc caggatcgaa gagatagtca acacccgggc caactacacc
     3001 gaacggttcg aggaggtcac gttccatgac agcagcggtg cctccgaaca gtacgagatc
     3061 tgccggagcg gctttgggat catggcggtt gcttcccggg caggatccct ccagatggga
     3121 tacgaacgcc ggcattccat cagggggctc aacatccgcc accagcagga tctcggccgt
     3181 tctgcagcgg agcgggcggt cgccctcctt tcagcccggc cggcacgggg aggaacgatg
     3241 aatgccgtcc tcgatcccga acttgcaggg gttttcgctc acgaggcggt gggacacgcg
     3301 agc
//
LOCUS       DAOOCO010000196         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_28541_length_3263_cov_4.409913, whole genome shotgun sequence.
ACCESSION   DAOOCO010000196 DAOOCO010000000
VERSION     DAOOCO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28541_length_3263_cov_4.409913"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..518)
                     /locus_tag="PK154_09400"
     CDS             complement(288..518)
                     /locus_tag="PK154_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11313.1"
                     /translation="MRPTLKEELEFAIWKITGTPMKFSEYTIPYLSQEIAKKTGEDPA
                     VVSLRLIQEMKQIINEDVDRQLKKCPPCMKRA"
     gene            complement(742..1623)
                     /locus_tag="PK154_09405"
     CDS             complement(742..1623)
                     /locus_tag="PK154_09405"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPW11314.1"
                     /translation="MIREKVLVLLLIMSLCGNVILVFFNPLLPPSFTGTNSTSGFAGI
                     DLSKISGHNDFMVDEYGNIIGIPPTQETPGVPNLTVPRGDSDLSSSDPEAEELNETAV
                     MITGDPTMTPTPEVTGEIPLDPLVTYENKKFGFSLRYPRNWTINEAAAGRTVLTLTAP
                     IERGCDSETSQCFNYIANLTVEIDQKPKTLILEDYFNSAVSQLQLDYFITTTSKSAPA
                     LLSDTRAYQIEYYTRDKRGNADRRYMQYYALMDGKGYIVSYFGPYSTWENVYDHNKPD
                     AQMVVNSFTVQRTFKTA"
     gene            complement(1741..1920)
                     /locus_tag="PK154_09410"
     CDS             complement(1741..1920)
                     /locus_tag="PK154_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11315.1"
                     /translation="MDKTGVASLIGGIILLALGAYAVWVFLPDVITVLKGSIGIIAII
                     IGIFLVIFGAILIKD"
     gene            complement(2029..2277)
                     /gene="hypC"
                     /locus_tag="PK154_09415"
     CDS             complement(2029..2277)
                     /gene="hypC"
                     /locus_tag="PK154_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPW11316.1"
                     /translation="MCIAIPAEVIEKKEGNIGIVDYGDLRQEVRLDLVDVEVGEFVLV
                     HVGFAIQRLSREEGFETREIFRQVFAAMEEEAEGQQTS"
     gene            complement(2713..>3263)
                     /locus_tag="PK154_09420"
     CDS             complement(2713..>3263)
                     /locus_tag="PK154_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW11317.1"
                     /translation="ATFINEQIPLVTQARRDVGINAIFLAVECNDDPDYYSGVGQYGD
                     YSVIESRFSPYTIPPGALADNISRFKQDFEKRWGGFPGMMGASTYEGVYIAAQAVENA
                     GTRDKAAVVTALHSLEMPEIIEEMEGGVIRFTPEFREAKFDLYMEQLRWNETARNLRP
                     VIVWPDHLKEGDFILPDWFEAV"
BASE COUNT          833 a          808 c          791 g          831 t
ORIGIN      
        1 caccatcata attcaggttt tgcgctggat ggctgagatt ctgatcgggg cacatgagtt
       61 cacaacggtt tcgcgcaggg aggcgaacgc gtggcatccg gataatccgg tcacgaggag
      121 gacgtcccgc taccgcccaa tgcccgtttt cttcaatggc aattacacgc tcctcccctc
      181 ggaatgcatg ataaaggtaa tacgggagga gggggagaga agaggatgtg aggggatgta
      241 tgggacgtgc gcttgtatga cgtatacccg aaaacagggg gagatgttca ggcccgtttc
      301 atacagggag gacacttttt cagctgtcga tcgacatctt cgttgatgat ctgcttcatc
      361 tcctggatca gcctcaggct aacgacggcg gggtcctcac cagtcttctt tgcaatctcc
      421 tgtgacagat aggggatggt gtattcagaa aacttcatcg gggtgccggt gatcttccag
      481 atcgcaaatt caagttcttc tttcagggtc gggcgcatgg atcacttccg ggggaataac
      541 caccatgaaa aaatgtcggt tctaattata ttactaagat tattttataa atcttattga
      601 tatggcagcc tgaattcaga cgtaagccca ttgctgcttt cccgggtttt cacggatcgc
      661 gcctaaggac ataatacaga aatcgttagc gaggtccgtt gcgaaaatca gggggatggc
      721 aggtccctgc tccccgcttc ttcaggctgt tttaaaggtc ctttgtacgg tgaaagaatt
      781 caccaccatc tgggcatccg gcttattatg gtcgtatacg ttttcccagg tggagtaagg
      841 tccgaaatag ctgacgatat agcctttccc atccatcaac gcgtaatact gcatatatct
      901 ccggtcagca ttgcctcttt tatcgcgggt gtaatattca atctggtagg ctcgtgtatc
      961 agaaagaagc gcgggtgcac tcttgctcgt cgttgttata aaataatcca gctgcagctg
     1021 agacactgca gaattgaaat agtcctcaag gatcagtgtc tttggtttct gatcaatctc
     1081 taccgttaaa ttggcgatgt aattaaagca ctggctggtc tcggaatcac agcccctctc
     1141 gatcggtgcg gtcagcgtaa ggacggtcct ccctgcggcc gcttcgttga tggtccagtt
     1201 tctcgggtag cgaagagaga acccgaattt cttgttctca taggtgacca ggggatcgag
     1261 cgggatctcc cccgtcactt cgggagtcgg cgtcatggtt gggtcaccag tgatcatgac
     1321 agccgtttca ttcagttctt ctgcttccgg atcggaagat gagagatcag aatccccccg
     1381 aggaacagtg agattgggta caccgggtgt ttcctgggta ggaggaatcc cgatgatatt
     1441 tccatactcg tcaaccataa agtcattatg acctgaaatt ttagaaaggt caatgcctgc
     1501 aaaaccggac gtgctgtttg ttcctgtaaa ggatggaggg aggagaggat taaaaaatac
     1561 aaggatgaca ttcccgcaga gagacatgat cagcagcagc acaagcacct tctcccgtat
     1621 cattgttgca cctcataaaa atgggattat atcctgttat atggataaat tatgacctta
     1681 taggccttaa aatttgacat cgcggtcagg tcaccctgca tgcaccagaa aaagatgtga
     1741 tcagtccttg atgagaatcg ccccgaatat taccaggaag attccgatga taatcgcaat
     1801 aatccctatg gatcccttca gaacggtgat gacatcaggg aggaataccc atactgcata
     1861 ggccccgaga gccagcagaa taattccccc aataaggctc gcaacacctg ttttatccat
     1921 ttcatactct ccttttacta ctctatcagt tctttgaata cataagaata gtgctgttga
     1981 agggagggtc acctggacga tcaggcatat cgcttgaaaa aaatagaatc aggacgtctg
     2041 ctgtccttca gcctcttctt ccatcgcagc gaatacctgt ctgaagatct cacgggtctc
     2101 aaatccttcc tcccgcgaga gcctctggat ggcaaaaccc acatggacga gtacaaactc
     2161 tccgacttcc acatcgacca gatcaagccg cacttcctgt ctcaagtcac cgtaatcgac
     2221 aatcccgata ttaccctctt tcttctcaat gacctcggca ggtattgcga tacacataga
     2281 gtcggatccc tgcattcata ttgttgttct gaaatgataa aagagatgat tgtggtggct
     2341 tatagcaaca aaaaaagaac tggctagcaa agatcctgag gaggaacgat tcaaaagggc
     2401 tacccgaccg ggtcccgcac gccattcgga atccccggcg atgtactggt tcatgattca
     2461 ggcgaggagt gccaaccagc attcctgggt tcagaagtat tttcttctta ggggtgggta
     2521 tacctcctta attctggcct catcattctc acatacagag gagagagtgg tttctcgcaa
     2581 gcgtttttcc ggaaaaaaca gatcctgaca tccggtacaa aacgggagtt gcctgtatga
     2641 aaaaaataat atgattcctt tgagggctac aagcataaaa gatgaaaatg ccggaggcat
     2701 tttcatataa aattagactg cctcaaacca gtcaggaagt atgaagtccc cttccttgag
     2761 atggtcgggc cagacgatta ccggccgaag gttccttgca gtttcattcc atcgcaactg
     2821 ttccatgtac aggtcaaatt tggcctccct gaactcgggg gtgaaccgga tcactccccc
     2881 ttccatctcc tcgataatct cgggcatctc aagtgagtga agagcggtaa ccaccgcagc
     2941 cttgtcacgg gttcctgcat tttcaaccgc ctgtgctgca atatagaccc cttcataggt
     3001 cgatgctccc atcatccccg ggaagcctcc ccacctcttc tcgaaatcct gcttgaaccg
     3061 ggaaatatta tcggctaacg ccccgggtgg gatggtgtac gggctgaacc gggactcgat
     3121 gaccgagtag tcaccgtact gtcccactcc gctataataa tcaggatcat cattgcactc
     3181 taccgcaagg aagatagcat tgatccctac atcccgccgt gcctgggtga caagggggat
     3241 ctgctcgttg atgaacgtcg ccg
//
LOCUS       DAOOCO010000197         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_28844_length_3241_cov_4.474576, whole genome shotgun sequence.
ACCESSION   DAOOCO010000197 DAOOCO010000000
VERSION     DAOOCO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28844_length_3241_cov_4.474576"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK154_09425"
     CDS             <1..685
                     /locus_tag="PK154_09425"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HPW11318.1"
                     /translation="HTRWKNWVHVTKLDPDKMLPAGAIAEIASSGTDALMLSGTLNVT
                     AENMAALAKEARASGLPLVVEPAGPEAVLWDGVDLVFVPSVLNSPEVQWIVGKHRSWI
                     QRQQVEWEKVVPEAYIVLNPDSAVGKLTKATCTLTGKEVASFASLADHYFHFPIVYIE
                     YSGTYGDPGVVREAASAVENAAIYYGGGINSAAKAAEMAKYADTIVVGNAVYDLGIGV
                     LKETVRAIQ"
     gene            698..1444
                     /locus_tag="PK154_09430"
     CDS             698..1444
                     /locus_tag="PK154_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW11319.1"
                     /translation="MPEIEIVLVSPLYEGNVGFAARVMKNFGFTRLVLIDPCKIGDEA
                     FMRAAHASDVLEKAERLSFEEVCARSSIVIATTGEVSKSVCNPMRMPYFSPNEIRELI
                     ADIDGRISILFGRENWGLNNEEIQRADIVCTIPSAKDYPILNLSHAVGIVCYELAHLP
                     RGEYLLATREEMEYLYAHIDSYLTLIHHPDFKRVPTMLLIRRVLGRAMLTSREATTLH
                     GLLRRSEWNINPAAFERRKEIPLDDPPKEW"
     gene            1668..1850
                     /locus_tag="PK154_09435"
     CDS             1668..1850
                     /locus_tag="PK154_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11320.1"
                     /translation="MRILAYGLAALALILMLCISGCTSPSPQMVTTPVPTTSPPTVQP
                     TPVTTQQAPQSLTGIT"
     gene            1908..2549
                     /locus_tag="PK154_09440"
     CDS             1908..2549
                     /locus_tag="PK154_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW11321.1"
                     /translation="MTAFFDAQGKVSGSAGCNQYTASYEATPVGLSIGAPASTKMNCN
                     EPAGIMTQETVYLSAIQGAATYSLSGDTLTIKDSGGKALLTYSTVPPYQMTPAPLTGT
                     MWYLNSYVDSKGKPWTTGSANPISIQFADDGKVSGNAGCNSYSGSYTVTGNSITMSGF
                     ATTLMYCGEPGVMDLESSYLGILPAMKVYKISGNELTLSDGVGTITLVYDTTR"
     gene            2658..3212
                     /gene="dcd"
                     /locus_tag="PK154_09445"
     CDS             2658..3212
                     /gene="dcd"
                     /locus_tag="PK154_09445"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPW11322.1"
                     /translation="MILVDWQILDRITRGFIHVDPFDPALVQPNSLDIRLGDHFLWYE
                     AGEEVIDPYRKETIASGTADCHADSLVLDPGRFLLAETMEVIGLPDNIVASIEGKSSI
                     ARLGVELHQTGGWIDAGFRGSITLEMCNVNARPVRLYAGMPIGQLVFYTTERAAQPYN
                     LKAGAKYMDQRQATLSRYHENPRS"
BASE COUNT          711 a          970 c          902 g          658 t
ORIGIN      
        1 gcatacccga tggaagaact gggtccatgt gaccaagctt gaccctgaca agatgctccc
       61 tgcaggggca atcgcagaga tcgcctcaag cgggaccgat gccctgatgc tctcggggac
      121 gctcaatgtg acggccgaga acatggcggc gcttgccaag gaggcacggg cgtcagggct
      181 cccgctggtg gtggaaccgg ccggccccga ggcagtgctc tgggacggcg tggaccttgt
      241 ctttgtcccc tcggtactga actctcctga agtccagtgg atcgtgggga aacaccggtc
      301 ctggatccag aggcagcagg tggagtggga gaaggtggtc cccgaggcat atatcgtgct
      361 gaaccctgat tctgcggtgg ggaagctgac caaggcaacc tgcaccctga caggaaaaga
      421 ggtcgcctcg tttgcctcac tcgctgatca ctacttccac ttccccatcg tctatatcga
      481 gtattccggg acgtacggcg atcccggagt cgtcagggag gcagcatcag cggtggagaa
      541 cgcggccatc tattacgggg gcggtatcaa ttcggccgcg aaagctgccg agatggccaa
      601 gtacgcggac actatcgtgg tcggcaatgc cgtctacgac ctgggtatcg gtgttctgaa
      661 agagacggtc agggcaatcc agtgatcctc cccttccatg cctgaaatcg agatcgtcct
      721 cgtctccccg ctctatgaag ggaacgtcgg atttgccgca cgggtgatga aaaatttcgg
      781 gttcacccgg cttgtcctga tcgatccctg taaaataggg gacgaagcat ttatgcgggc
      841 agcccacgcc tccgatgtac tcgagaaggc cgaacgactc tcttttgaag aggtctgtgc
      901 ccggagcagc atcgtgatcg cgaccacggg cgaggtgagc aagtcggtat gcaacccgat
      961 gcggatgccc tatttctcac ccaacgagat acgggagctg atcgctgata ttgatggacg
     1021 catctccatc ctgttcgggc gggagaactg gggactgaac aacgaggaga tccagagggc
     1081 cgacatcgtc tgcactatcc cctcggcaaa ggattatcca atcctcaatc tctcgcacgc
     1141 ggtcgggatc gtctgctatg aacttgccca tctcccaagg ggtgaatacc tccttgccac
     1201 gcgggaggag atggagtacc tgtacgccca tatcgattcg tacctcaccc tgatccatca
     1261 cccggatttc aagcgggtcc ccaccatgct cctgatcagg agggtccttg gccgggccat
     1321 gctcacctcc cgcgaggcaa cgaccctgca tgggctgttg cggcgatccg agtggaatat
     1381 caacccggcg gctttcgagc ggagaaagga gatcccgctg gatgatcccc caaaggagtg
     1441 gtgaggctga acaccctgaa taccagcctc atcgaaccgg cgaccgctac gcggggattc
     1501 gggaatgctt ttttcccctg aaatcctgat accatccgga ccggaatctt tgcctgtcat
     1561 cagggattga gcctgcagta tccctgcaag gatgtcccca gagcagccag ctgatctccc
     1621 atcatttatc cacaggactt aaatagcgaa attccatatc acctcttatg agaatcctcg
     1681 cttatgggct tgcggcactc gccctcatcc tgatgctctg catcagcgga tgcacgtcac
     1741 catcacccca gatggtgacc acccctgtac cgaccaccag tccgcccact gttcagccta
     1801 cccctgtgac cacgcaacag gccccgcagt cacttaccgg cattacctag tatctcgtct
     1861 cactgaacga gggggatgca tcactctcgg taaaaccggg aaccacgatc actgcattct
     1921 tcgatgccca gggcaaggta tccggatcgg caggatgcaa ccagtatact gcttcctacg
     1981 aggcgacccc tgtagggctt tcaatcggcg caccggcatc gacaaagatg aactgcaatg
     2041 aacctgccgg gatcatgacc caggagacgg tatacctgtc cgccatccaa ggtgcagcaa
     2101 cctattctct ctctggtgat accctgacga ttaaagacag cgggggaaaa gccctcctca
     2161 cctattcgac ggtccctccc taccagatga ctcctgcccc actgaccggc acgatgtggt
     2221 atctgaactc gtatgtcgat tcgaagggaa aaccatggac aacggggagt gcaaatccca
     2281 tatcgatcca gtttgccgat gacggaaagg tgagcggaaa tgcagggtgc aacagctact
     2341 ctggatccta caccgtcaca gggaacagta tcaccatgag tggatttgcg accacgctga
     2401 tgtactgcgg tgagccgggg gtcatggatc tcgagagctc atatcttgga atcctcccgg
     2461 cgatgaaagt ctacaagatc tccggaaacg agctgaccct ctctgatggt gtcggaacga
     2521 tcacgctggt ctacgatacc accaggtgaa atcctccatt cccccttttt cctgaaccag
     2581 cgcttgcctg ttttccacgt gaagagtggg ggctgttcag acaccctcat tacctctccc
     2641 cgcccatatt gaggccgatg atccttgtcg actggcagat cctggaccgg ataacccggg
     2701 gttttatcca cgtggatccc tttgaccctg ccctcgtcca gcccaattcg ctcgatatcc
     2761 gcctcggaga ccatttcctc tggtacgagg caggggagga ggtgatcgat ccgtaccgga
     2821 aagagaccat cgcgagcggc acagcggact gccatgccga ttcgttggtg ctggatcccg
     2881 gccgtttttt gcttgccgag acgatggagg tgatcgggct ccccgacaac atcgtggcaa
     2941 gcatcgaggg gaagagcagc atcgcacgcc tcggggtcga gctccaccag accggggggt
     3001 ggatcgatgc cgggttccgg gggtcgatca ctctcgagat gtgcaacgtc aatgcacgcc
     3061 cggtccggtt gtatgcaggg atgccgatcg gccagctcgt cttctacacg accgagcggg
     3121 cagcccagcc ctacaacctg aaagcaggtg ccaagtacat ggaccagcgg caggcaaccc
     3181 tctcccggta ccacgagaat ccccgctcct gatggatata taccttatga atacagacat
     3241 a
//
LOCUS       DAOOCO010000198         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_29648_length_3181_cov_4.418746, whole genome shotgun sequence.
ACCESSION   DAOOCO010000198 DAOOCO010000000
VERSION     DAOOCO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29648_length_3181_cov_4.418746"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..802)
                     /locus_tag="PK154_09450"
     CDS             complement(455..802)
                     /locus_tag="PK154_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11323.1"
                     /translation="MQTGFGWIEVGKRTYPHDIIIHTDGSVSKRKKKVSKGMKGDYGH
                     TPLSEFELGFLSDEKPEVVYIGTGQHGSLPVTAEAEEILSSFITVRLPTSEIIRFIEE
                     EKRSFVALLHITC"
     gene            complement(894..1289)
                     /locus_tag="PK154_09455"
     CDS             complement(894..1289)
                     /locus_tag="PK154_09455"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW11324.1"
                     /translation="MIRKDKPVTARGETRAVDAYISTFPADIRVILEQLRKAIHESAP
                     GAQEAISYGIPTFMFHGTLVHFGAFEQHIGFYPTPSAIRAFEDELRPYPHAKGSIRFP
                     LGKEIPLDLVRKMVRYRVEENLALAKAKG"
     gene            complement(1353..2564)
                     /locus_tag="PK154_09460"
     CDS             complement(1353..2564)
                     /locus_tag="PK154_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPW11325.1"
                     /translation="MKSMQYRTVPKNGDRLSTLGYGTMRLPTRVGRIDEERAIRQIRS
                     AIDRGLNYIDTASPYHGGESERVVGKALADGYREKVLLATKLSPWDVRTRGDMDRILG
                     EQLEKLRTSCIDYYLLHSLDAIQWKKLRDLGALEFLDSACASGRIRNAGFSFHGDLGT
                     LREIIDAYPWIFCQIQYNFLDEQNQAGTEGLRYAASRGIAVMVMEPLRGGMLARNLPS
                     KIRQEYARSPVRRSAAAWALRWVLNHPEVTVVLSGMNDEGHIDENLKTCNDALPGSMS
                     AEELETIRNVAGLYNRLTKVACTGCSYCIPCPFGVNIPYCLSLYNDYCRGDSRLITRG
                     WYGMRLMGGQGGGRADASLCRNCGRCAQVCPQKIPIPDELKKVNRTLGGIRTQIMLPF
                     IRMILRRKKKE"
     gene            complement(2669..>3181)
                     /locus_tag="PK154_09465"
     CDS             complement(2669..>3181)
                     /locus_tag="PK154_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPW11326.1"
                     /translation="FIRQSKDPSLYTIVLEDSAAMLGLIFAFLGIFFGHLLNNPYLDG
                     IASIAIGLLLMSVAWILASRTMGLLMGEGVNPEQLADIRRRVETDPAVERAGDILTMY
                     MGPHDLLVNMGVCFAEGTTAEQMHESIRRIEADLRGAYPETNRVYIEAESLPAGLSRK
                     GEERCRNRDS"
BASE COUNT          718 a          906 c          813 g          744 t
ORIGIN      
        1 cgcctgggag ggtgagggtt actgtatcct cggccagtac agggatgata aagaatccca
       61 gcactgccat tgctataaat aatatcggta atttccccgt catttaaatc gttcaatggt
      121 aataatttca ataaataaaa atcttttgga gaattgaggg attcagaggc tcatttgagt
      181 cataagaacc cctattaatt ggatttacat cggctgcgac gggcatgaga ttacgtcggc
      241 aaacccccta aaaaggacgc tccggtccaa tcctggtccg tctggatttt cctgccagac
      301 cgcgaggtgc cgcaaggcct ggcaatggcc agagcgtatt gtctgcgacc aggaaccctc
      361 cctgatccaa aaaagcgagt ggtgattgac ccccaccgta tccacaccag cacgagcctc
      421 atcgggtgtt atcccaatat agataaagtc atactcagca ggtgatgtga aggagagcca
      481 caaacgatcg cttttcctcc tcaatgaacc ggataatctc gctggttggc aggcgcaccg
      541 tgataaatga agagagaatc tcttctgctt cagcggtgac gggaagactg ccatgctgcc
      601 cggtcccgat atacaccact tcgggttttt catcgctgag aaagccgagc tcgaattctg
      661 aaagaggagt atgcccatag tctcctttca tccccttgga gaccttcttc tttctcttcg
      721 ataccgaacc atcggtatgg atgatgatat catgggggta ggttctcttt cccacttcga
      781 tccagccaaa tccggtctgc atcagcgatc gttcccctcc ctggtgtcca atccgatcac
      841 ccctctgccc cttccctcac tatcactctt ccggttatga aaggggaagg atatcatccc
      901 ttcgcttttg ccagggcaag gttctcttct accctgtacc ggaccatctt cctgacaagg
      961 tcaagaggaa tctctttacc aaggggaaac cggatcgagc cctttgcatg gggataaggt
     1021 ctcaactcat cctcaaaagc ccggattgca gatggggtgg gatagaaccc gatatgctgc
     1081 tcaaacgctc cgaaatgcac gagagtgcca tggaacatga aggtggggat gccataactg
     1141 atcgcttcct gtgcaccagg tgcactctca tggatcgcct ttcgtaattg ttcgagaatc
     1201 acgcggatgt cggcgggaaa ggtactgata tacgcatcaa ctgcccgggt ttctcccctc
     1261 gctgtgacgg gcttatcctt cctgatcata cgattctcac tatcttcacg tgatacagcc
     1321 cccttgttcc ggggtataaa gtctcttttg ggtcattcct tttttttcct tcggagaatc
     1381 atcctgatga aagggagcat gatctgggta cgaattcccc caagcgtccg gttgactttt
     1441 ttcagctcat ccgggatggg aatcttctgg gggcagacct gggcgcacct cccgcagtta
     1501 cggcagagtg aagcatcggc acgtcccccg ccctggccgc ccataagccg catcccatac
     1561 cacccccggg tgatgagtcg ggaatctccc cgacagtagt cattgtacag ggagaggcaa
     1621 taggggatat tcaccccgaa cgggcagggt atgcagtacg aacagccggt gcatgccacc
     1681 tttgtaagac ggttgtacag tcccgccacg ttcctgatcg tctccagctc ttcagcgctc
     1741 atcgatccgg ggagcgcatc gttgcaggtc ttcagattct catcgatgtg cccctcgtcg
     1801 ttcatccctg acaggacgac cgtcacttcg gggtggttta aaacccagcg tagtgcccag
     1861 gcagcagctg aacgccggac aggggaccgt gcgtattcct gccgtatctt tgaggggaga
     1921 ttacgggcaa gcatgccgcc ccggagcggt tccatcacca tcactgcaat cccccttgat
     1981 gctgcatacc ggagaccttc ggtcccggcc tggttctgtt cgtcaaggaa attgtactgg
     2041 atctggcaga atatccaggg ataggcatcg atgatctcac gaagcgtccc gagatcgcca
     2101 tggaaagaga agccggcatt ccgtatcctt cccgaggcac atgcagagtc caggaattcc
     2161 agggccccaa gatcccggag tttcttccac tggatggcat caagcgagtg gaggaggtaa
     2221 taatcaatgc aactggtccg gagtttttcc aattgttctc cgagaatcct gtccatatct
     2281 ccgcgtgtcc ggacgtccca gggggagagc ttggtagcaa ggagcacctt ttccctatac
     2341 ccgtctgcaa gggctttgcc caccacccgt tcgctctcac cgccatggta gggagaagcg
     2401 gtatcgatgt agttcaatcc gcgatcgatg gcactccgga tctggcgtat tgctcgctcc
     2461 tcatcgatcc tccccaccct tgtaggaagc cgcatcgtac catagcccag ggtggacagg
     2521 cgatcaccat tctttggtac agtccggtac tgcatggact tcatccctgt tgcacctgga
     2581 tcgattcccg gccaggcagg tacaagtatc tcaggaattc ggtagtgata tcaaaaaaga
     2641 ctgtgctatc cggaaaggga ggagaggatc aactatccct gttcctgcag cgctcctctc
     2701 ctttcctaga gaggcctgcc ggcagcgatt cggcctcgat gtagacacga ttggtctccg
     2761 ggtaggcacc ccggagatct gcctcgatcc ggcgtatcga ctcgtgcatc tgctcagccg
     2821 tggtaccttc agcaaaacat acccccatat tcaccagcag atcatggggc cccatgtaca
     2881 tcgtcaggat atctcccgcc cgttcgactg cagggtccgt ttcgacgcgc cgccggatgt
     2941 ccgcaagctg ttcgggattc accccctccc ccatcagcag gcccatggtc cgtgatgcaa
     3001 gaatccaggc cacgctcatc agcaacaggc cgattgctat tgaggcaata ccatcgagat
     3061 agggattgtt caggaggtgc ccaaagaaga tcccaaggaa agcaaagatc agcccgagca
     3121 ttgcagcgct gtcctcaagg acgatggtat agagactggg atctttcgac tgcctgatga
     3181 a
//
LOCUS       DAOOCO010000199         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_30091_length_3150_cov_3.492084, whole genome shotgun sequence.
ACCESSION   DAOOCO010000199 DAOOCO010000000
VERSION     DAOOCO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30091_length_3150_cov_3.492084"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK154_09470"
     CDS             complement(<1..215)
                     /locus_tag="PK154_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11327.1"
                     /translation="MRGKDIIFRLALRSIRLHFLRSTLAVLGIVIGVVAIATMGMMGA
                     NMTLSVTEQLSEMGNVLVVTESREGGGG"
     gene            complement(212..886)
                     /locus_tag="PK154_09475"
     CDS             complement(212..886)
                     /locus_tag="PK154_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11328.1"
                     /translation="MDDQPLIELRGVTKVYPLKSGDVTALDNISLTIQRGDFVAIMGP
                     SGSGKSTLLNQLGCLDRPTSGDLLIAGRNTRAMDDHELTGLRLNTIGYIFQKFNLIPL
                     LSAYENVEYPYLMKHRQEDEHGHISRLLSKVGIDAALARHKPPELSGGQQQRVAIARA
                     LVNDPQILLCDEPTGNLDSATGGQIMEMIRTLNEQGRTVLMVTHDPTVAACAKRNVII
                     KDGRIT"
     gene            complement(904..2196)
                     /locus_tag="PK154_09480"
     CDS             complement(904..2196)
                     /locus_tag="PK154_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11329.1"
                     /translation="MMKGKRTATRRYLILSLLVLLLSLGSICTLVSAADSTTEASSEA
                     YVAITNVTLDPSILMQYDQGTLAIRITNSGSSPVKINRVELLSEELDVVNYQTYDKVG
                     VLGASNSLVFTFLVEADVKDATYFPIFYVDFTDSGSMRYPIAVKVDDTPLLVSVVKSP
                     DTFTSGEEDKITLSISNPRDNEVNSVTVVPGGDGISPSQSAVFVGTLKPDEERQVTFG
                     ITPEKATVLVLDVLYRNGPNQHNETITLPVMLGNRKVAAELVVNNIEVAGGGASLTIS
                     GDVTNAGLKDAKSVTVTVGDPAWAIDPDPVYVVGALEPDDFSSFEITCKAPGLSQIPL
                     VVSYRDDEGETYQETFDVSALSFGNSTAGGSADGGFQGPPSMQGRPSGGGMGMMGFGS
                     GFSKIPVIPILIVIIAIIGVLVAWRKGYLEKIRERFSK"
     gene            complement(2627..2827)
                     /locus_tag="PK154_09485"
     CDS             complement(2627..2827)
                     /locus_tag="PK154_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW11330.1"
                     /translation="MKNRIKVFRAMHDLTQDALAQQLGVTRQTILAIEKGKYDPSLEL
                     AFKIARFFNVSIEEVFSYPGAP"
     gene            complement(2811..>3150)
                     /locus_tag="PK154_09490"
     CDS             complement(2811..>3150)
                     /locus_tag="PK154_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11331.1"
                     /translation="FSYVRHSWSTMVIFFATSLGSAVIGLGAPPFPHFPRPPDQGPSR
                     INESIKPFGDGQGLPGESIRPPDDGLIRLGHFGYLQLALLFLMFFLYVGFRIYYARKY
                     GEWESDEEQD"
BASE COUNT          711 a          832 c          842 g          765 t
ORIGIN      
        1 cccccaccac cttcccggga ttcggtgaca accaggacat ttcccatttc cgagagttgt
       61 tcggtcaccg agagcgtcat gttcgccccc atcatcccca ttgtggcaat ggcgacgacc
      121 ccgataacga tcccgagaac agccagcgtg gatctgagaa agtggagccg gatactccga
      181 agagcaagcc ggaaaatgat gtccttgccc ctcatgtgat cctgccgtcc tttatgatga
      241 cgttcctttt tgcacatgct gcaacggtcg gatcatgggt taccatcagc acagtccgtc
      301 cctgttcgtt cagcgtccgt atcatctcca tgatctgtcc cccggtcgcc gagtccaggt
      361 tgccggtggg ttcatcgcag agaaggatct ggggatcgtt gacgagggcc cgtgcgatgg
      421 caacccgctg ttgctgaccc cctgagagtt ccgggggttt gtgcctggca agtgcggcgt
      481 caatcccgac cttactgagt aaccgggata tgtgaccatg ttcatcctcc tgccgatgtt
      541 tcatcaaata gggatattcg acattctcat aagcggaaag gagggggatg aggttgaact
      601 tctggaagat gtacccgatg gtgttcaagc ggagtcctgt gagttcgtga tcatccatcg
      661 cccgggtgtt tcttcctgca atcagaagat cgccggaagt agggcgatcg agacatccaa
      721 gctggttcag gagggtggat tttcctgacc cggaagggcc catgatagcc acgaaatccc
      781 ccctctggat ggtgagcgag atattatcga gtgcagtaac atctccggat ttcagcgggt
      841 ataccttggt gacaccccgt aactcgatca acggctggtc atccattccc ctcactccag
      901 ggatcatttg ctgaaccgtt cccggatctt ttccaggtat cccttacgcc atgctacaag
      961 aacaccgatg atggcgataa tgacaatgag gatcgggatg accgggatct tactgaagcc
     1021 actgccaaat cccatcatcc ccattcctcc gccacttggg cgaccttgca ttgaaggagg
     1081 cccctggaag cccccgtctg ctgatccccc agcggttgag ttcccaaagc taagtgccga
     1141 aacatcgaac gtctcctggt aagtctctcc ttcatcatcg cggtacgata cgacaagggg
     1201 gatttgggac agtcccgggg ctttacaggt gatctcaaaa ctggaaaagt catccggctc
     1261 aagagctccc accacataga ccgggtctgg atcaattgcc caggcagggt ctccaaccgt
     1321 taccgtcact gattttgcat ctttcagtcc ggcattggtg acgtccccgg agatggtcag
     1381 gctcgcccct cctccggcca cttctatgtt gttcaccacg agttcagcag caaccttcct
     1441 gtttcccagc atcaccggca gggtgatggt ctcgttatgc tggttcggac catttcggta
     1501 gaggacatcc aggacaagta ccgtagcctt ctctggggtg atcccgaagg tgacctgcct
     1561 ctcttcgtca ggtttgagcg tcccgacaaa tacggcactt tgcgacgggc tgataccgtc
     1621 cccgcccggt acgaccgtca cgctgttcac ctcattatcg cggggattgc ttatcgagag
     1681 ggtgattttg tcctcctcac cggaggtgaa ggtgtccggc gatttgacaa ccgagaccag
     1741 gaggggggta tcgtcaactt ttaccgcgat cgggtagcgc atactccccg aatcggtgaa
     1801 gtcgacatag aatatcggga aatatgtcgc atccttgacg tcagcctcga caaggaacgt
     1861 gaagacgagg gaatttgacg ccccgaggac tcccacctta tcgtaggtct ggtagttgac
     1921 tacatcaagt tcttcggaaa ggagctcaac acggttgatc ttcaccgggg atgatcctga
     1981 attggttatc cgtattgcca gtgttccctg gtcatactgc atcaggatcg agggatcgag
     2041 ggtaacattc gtgattgcga catatgcttc tgacgatgcc tcagttgtag aatctgccgc
     2101 cgagacaagg gtacagatgc ttcccaggct gagaagtagt acaaggagag agagaatcag
     2161 atatcttctg gtagcggttc ttttcccttt catcataatg gttctgcctc atatgcgttt
     2221 gttagggttt aacacaaggg cggtgttctt gagaggatcg gatatcggtc cattcaggaa
     2281 catggaggga aaaggtggct ttgctcccac cggggttatt tgtaggagga tcaaagaccg
     2341 atgcgctcaa tattgattcc gggtcccggc ctctcatccc acagtttcat ggttgtacat
     2401 gagaagattg cggaatccgg gatcggtttt tttggatcgg gctatccggt ccctcccata
     2461 ttcctaattt gcatggagtg attgttccat gccggattgg ccggcacgta tagtatattt
     2521 tacattatgt tatataaaat ctaccaatag aatcccaatt caggagggag tatatgatga
     2581 aaccagtgag gacgtctgat gactcttcga ctgcatatca caactgttaa ggggccccgg
     2641 gataggagaa gacctcttca atactcacgt tgaagaaacg ggcaattttg aaggcaagct
     2701 cgagagatgg atcatacttt cccttctcaa tggcaaggat agtctggcgg gtgaccccca
     2761 gctgctgtgc aagggcatcc tgggtgaggt catgcatggc ccgaaagact ttaatcctgt
     2821 tcttcatcgc tctcccactc tccgtatttc cgggcataat agatcctgaa cccgacatag
     2881 aggaagaaca tcaggaagag gagcgccagt tgcagatacc caaagtgccc aagccggatt
     2941 agaccgtcat cggggggtct gatgctctcg cccggaagtc cctggccatc tccaaacggt
     3001 tttatgctct cattgattct gcttggtccc tggtccggcg gtcgtggaaa gtgtgggaag
     3061 gggggagcgc caagaccgat gacagcactc cccaggctgg ttgcaaaaaa aatcaccatg
     3121 gtcgaccatg agtgtctgac atacgaaaat
//
LOCUS       DAOOCO010000200         3132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_30337_length_3132_cov_4.078323, whole genome shotgun sequence.
ACCESSION   DAOOCO010000200 DAOOCO010000000
VERSION     DAOOCO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30337_length_3132_cov_4.078323"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK154_09495"
     CDS             <1..556
                     /locus_tag="PK154_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11332.1"
                     /translation="LIRIIIIAPSILTSTLDFVDKLDYDINLIEVKMWNDDQNQILYV
                     HFLKEEERMKRRPVRGLETYDEKFYLTNYNKESARSFLDYTKEIETFVKSRGWPLELK
                     YTKYYAGFKAGRLNVFGIEWGGSKKIQIFVKISKEQARDLPMKMTDYNDTWKQAVFVL
                     EPGKTKIQEYFPIFEYSFKKFIGK"
     gene            complement(796..1173)
                     /locus_tag="PK154_09500"
     CDS             complement(796..1173)
                     /locus_tag="PK154_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11333.1"
                     /translation="MSILLFSGKLNGQDIHIRATIDGSTGDLVIAGHDIGPLCEKFVG
                     KDEYEYWLRIKSEYKERVIEHLEQDLENKYPMSEADAREENARDRYLLALIFITHAHD
                     PHCFESFREWCEGKEIPTHFANY"
     gene            complement(1222..1809)
                     /locus_tag="PK154_09505"
     CDS             complement(1222..1809)
                     /locus_tag="PK154_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW11334.1"
                     /translation="MKEIPAMNAHAIAIATQAATVAHWGQTRKDGTTPYIVHPERVAA
                     CVGRCGGDHVGMMAAWLHDVIEDCADGDQVVRRTLQKTDLPEEDRVAVYAIVAAMTKN
                     PAIPDKGERLMDSIRRINRAPPQAILVKLCDRTDNLADARSLGKEFLQTYLHLTDRLV
                     EELSDGAIRNGYTDALEQLKRARDDAKAWCEEKGS"
     gene            complement(1806..>3132)
                     /locus_tag="PK154_09510"
     CDS             complement(1806..>3132)
                     /locus_tag="PK154_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11335.1"
                     /translation="TSLFGGTELGVALEQALRQPRQPGEYSRHVLVITDGQVTDEGRL
                     FRLVGLEAVAPSSRRVSVISIDTAPHAHLALELARIGGGVAKFLTSSEEVDSVLKELL
                     ASWQPPVLSNAVLTIDRPGIEVNDYRVLSKSGKEMVDVGDLRPETPVFLCSRIPCSST
                     HPVVTLSNTEGVPITVAPARPGDGDLAGSLKVIFGAGRLRTLEYLMNAGYPDEELVRH
                     LEVAGYATLSPENPLYPENRRQAIHDALERLLVSESLRYGIPSTLTAFVGISDKAGTV
                     PPVTVAVPNALPAGWGIHEMCVDLCMDHMDMIMQSDVQMSLKRSPRVAESLIVTPKDI
                     DIPALEVKNGTAVLVDACPIAKGSYTLFLNFATRLRPAGVSVRIEIDGKVIKTWSDAK
                     LRPGDAKIMIPVHVSRSGRIRILITDPQGAWQGLKLSVSIRKEEGVMWV"
BASE COUNT          750 a          815 c          797 g          770 t
ORIGIN      
        1 cctgattcga attattatta ttgcgccttc catattaaca tccacacttg acttcgtgga
       61 taaactcgat tacgatatca atttgatcga agtgaaaatg tggaatgatg atcaaaatca
      121 gattttatac gttcattttc tcaaagaaga agaacgaatg aaacgcagac cagtgagagg
      181 tctggaaacc tatgacgaaa aattctattt aaccaattac aataaagaaa gtgctcggtc
      241 ctttttagat tatacaaaag agattgaaac cttcgtaaaa agcagaggat ggcctcttga
      301 attaaaatat accaaatatt atgcagggtt caaagcggga cgtttgaatg tttttgggat
      361 agaatgggga ggaagcaaga aaattcagat ttttgtaaaa atctctaaag aacaagccag
      421 agatttaccg atgaaaatga ctgattataa tgatacctgg aaacaagcgg tcttcgtact
      481 ggaaccgggg aaaacaaaaa ttcaggaata ttttccaatt tttgagtatt cctttaaaaa
      541 attcattgga aaatgatttt tcgttaatcc attcacctct cctacctgca atattttttt
      601 accggtgcat taattttttt aatgtacgcc ttatatcgga attgtcaaca tgtaatggaa
      661 aaaatcgtta ttattattga tctctggtta acaaaatcaa atccacagaa ggataaaaac
      721 gaaggatttg aaggcgaatg aaaaatgttt aatttggatt gaattttctc caattgaggt
      781 ctttttgggg ctaaattaat aattggcaaa atgagtaggg atctcctttc cctcgcacca
      841 ttccctgaac gattcgaaac aatggggatc gtgcgcatgt gttatgaata ttaatgcaag
      901 gagatatcga tccctggcgt tctcttctct ggcatcagct tcactcattg gatatttatt
      961 ttcgagatcc tgctccaggt gttcgattac tcgctccttg tattcgcttt tgatgcgtaa
     1021 ccagtattca tactcatcct ttcccacaaa tttctcgcac aaggggccga tatcgtgccc
     1081 tgcaataaca agatcgcccg tcgatccgtc tattgttgct cggatatgaa tatcctggcc
     1141 attgagtttt cctgaaaata agagaatact catcactgga ttatagaggg gactgctcat
     1201 ggctatcact gattcccgtt ttcacgatcc cttctcctcg caccaagcct ttgcatcatc
     1261 ccgtgctctc ttcaactgtt ccaaagcgtc ggtatacccg ttccggattg ccccatcgga
     1321 cagctcctcg accagccgat cggtcaggtg gaggtatgtt tgaaggaact ccttcccgag
     1381 actacgggca tccgccaggt tatcggtccg gtcacataat ttgaccagga tggcctgggg
     1441 aggtgcccgg ttgatccgcc gaatggaatc catcagccgt tcgcccttgt cggggattgc
     1501 cgggttcttg gtcatggcag cgacgatagc gtagaccgcg accctgtcct cttcaggaag
     1561 atccgtcttc tgcagggtcc tccggacaac ctggtcgcca tcggcacaat cctcgattac
     1621 gtcatggagc caggccgcca tcatcccgac atggtccccg ccgcatcgcc cgacacatgc
     1681 tgctacccgc tcgggatgga caatataggg ggttgttcca tccttcctcg tttgtcccca
     1741 atgggcgacg gttgctgcct gtgtggcgat tgcaatggcg tgtgcgttca tcgccgggat
     1801 ctccttcata cccacatgac cccttcttcc tttcgtatcg agaccgagag cttcagcccc
     1861 tgccaggccc cctggggatc ggtgatgagg atccgtatcc ttcccgaacg ggagacgtgg
     1921 accgggatca tgatcttcgc atccccggga cgaagcttgg catcgctcca ggtcttgatc
     1981 accttcccat cgatctcaat cctgaccgag accccggcag gacgaagcct ggtggcgaag
     2041 ttcaggaaca gggtgtacga tcctttcgca atcgggcagg catcgacgag gacggctgtt
     2101 ccgttcttca cctcgagggc agggatgtcg atgtccttcg gggtcactat cagagattct
     2161 gcgactcttg gggaacgctt aagggacatc tgcacgtcag actgcatgat catatccatg
     2221 tggtccatgc atagatcaac acacatttca tgaatccccc acccggccgg gagagcattg
     2281 ggaaccgcga ccgtaaccgg ggggaccgtc cccgccttat cggaaatgcc gacaaatgcc
     2341 gtgagggtgg agggaattcc ataccggagc gattcggaga cgaggagccg ttcaagggca
     2401 tcatggatcg cctgccgccg gttctcggga tacagggggt tctccggtga aagggtggca
     2461 tagccggcca cttcgagatg ccggaccagc tcctcgtccg gatacccggc attcatcagg
     2521 tattcgaggg tgcggagccg gccggccccg aagatcacct tcagggatcc ggcgaggtcg
     2581 ccatcccccg gccgtgcggg agcgaccgtg atcggcaccc cctcagtgtt actcagggtt
     2641 accaccgggt gagtggagga gcacggaata cgggaacaga ggaacaccgg ggtttccggc
     2701 cggaggtccc cgacatctac catctccttc ccggacttgg aaagaacccg gtagtcattc
     2761 acctcgatcc ctggcctatc aatggtaagg accgcattgg agaggaccgg gggttgccat
     2821 gaggcgagca attccttaag gaccgaatcg acctcttcgc tgctcgtaag gaattttgcc
     2881 acgccacccc cgatccgtgc cagctcaagc gccaggtggg catgtggtgc ggtatcgatg
     2941 gagatcacgc tcacccgtcg ggacgacggt gctaccgctt ctagtccgac cagccggaag
     3001 aggcggccct catcggtcac ctggccatcg gtgatcacga gcacgtggcg tgagtactcc
     3061 cctggctggc ggggctgtcg gagtgcctgc tcgagggcga ccccgagctc agtccctcca
     3121 aagagcgagg tc
//
LOCUS       DAOOCO010000201         3098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_30822_length_3098_cov_4.220177, whole genome shotgun sequence.
ACCESSION   DAOOCO010000201 DAOOCO010000000
VERSION     DAOOCO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30822_length_3098_cov_4.220177"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK154_09515"
     CDS             complement(<1..269)
                     /locus_tag="PK154_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HPW11336.1"
                     /translation="MNFSQLHKNKKAQVVLGLCFGIVFGFLLQKGGATSYDVIMGQLL
                     LTDFTVLKIMASAVAVGLVGFFLLNHAGYARRHVKAGAIGSNVVGG"
     gene            complement(284..805)
                     /locus_tag="PK154_09520"
     CDS             complement(284..805)
                     /locus_tag="PK154_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPW11337.1"
                     /translation="MNGFLALPLWSPYLVGVCIGLLVCLALVLSDRPIGCSTGFVKIR
                     GLAEQICCGKQIQEKEYYQKFPPAVDWQLIILPGIIIGAFLSSFISGTFGLFIVPPLW
                     ADQFGSSAALRILAAVAGGILLGVGARWAGGCTSGHGISGSLQLSVGSILSAACFFVG
                     GIAMAMILFGVPL"
     gene            complement(1309..2697)
                     /locus_tag="PK154_09525"
     CDS             complement(1309..2697)
                     /locus_tag="PK154_09525"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW11338.1"
                     /translation="MMVILFLALAFCGICSGESNTQPQFPHMFYGTATIMGSPAPEGY
                     TITTTVDGAANGMISVSPAGTFGGEGALDQKLIVQGDFQASTSRITFAIEGSPALCRE
                     YGSGEGWQESYPFTPGAVTNLELNAGEVALLADFSVNRPDGYAPHTAQFADQSTGSHD
                     QWLWNFGDGKTSAEQNPSHTYTTAGTYTVSLTVISGTLTNVRTKSNYITVFAQGSPSG
                     GGGGGGGGGGGGSGTVITSGQTATTTMTGTLTPSPTVTRMSAGNLLLGTDNTTTREVT
                     IAATDTIGSLAIGKGVAPVDSTGAPLSTVSLSALPPGTIPPKSPCIAAPYQYEITPAG
                     VRFTSGVTLVLSFNETTWAGLDKEGASLARYNTTDASWEEIATSMDPGSRTMKAAVTR
                     GGTYVLCLDEGAQPLPTTEPPVPPVSTGSPGLPLGIIALASLLIIVVVGVIAYLISTR
                     TTNEGSPPEGGQ"
BASE COUNT          714 a          845 c          856 g          683 t
ORIGIN      
        1 ccgcccacca cattcgatcc gatggcaccc gcctttacat gccttcgtgc atacccggca
       61 tggttgagga ggaaaaatcc cacgagcccc accgcaacgg ctgatgccat gatcttgaga
      121 acggtaaagt cggtgagcag gagctggccc atgatcacgt catagctggt ggcccctccc
      181 ttctgcagga ggaatccgaa cacgatgcca aagcagagac cgagaaccac ctgggctttt
      241 ttatttttat gcaactggct gaaattcatt gccgaccagc tcctcacagg ggaactccaa
      301 agaggatcat cgccatggcg atcccgccca caaagaagca ggcggcggag aggatgctgc
      361 caaccgagag ctgcaggctt ccgctgatac catgaccgct tgtgcagccg cctgcccacc
      421 gggcccctac cccgagcaga atccctccgg caacggcggc aaggatcctg agtgctgcgg
      481 aacttccaaa ctggtcagcc cagagcggag gaacaatgaa gaggccaaat gtccccgaga
      541 tgaaggaaga caggaatgcc ccgatgatga tgccagggag aataatgagc tgccagtcaa
      601 ctgcaggagg aaatttctga taatattcct tctcctggat ctgtttgcca caacagatct
      661 gctctgcaag gccgcggatc ttgacaaaac ccgtggaaca accgatcggc ctgtccgaca
      721 ataccaaagc aaggcacact aggagcccga tacacacccc gactagatag ggtgaccaga
      781 gtgggagagc aaggaatccg ttcatactgt cctcaaagga tacgcgtggt tttaaggata
      841 cccgtggttt ttcccggaat accgtctcat tttggtattc ctgttggata caaaagaaga
      901 aacatcacca tttttcttaa tagtaactgt cagggcgggt atcggcaacc tgttttcgtc
      961 catccaggaa tggacaaccg ccatgacctt aggactatgt gctctaatga gatggtcatc
     1021 cgatctgcac gctggccagg gaccagggag caggtggcga gatgacggat tccggatggg
     1081 gaagtagtgc cgggagtgaa atgaacgatc gttcccgtac cagaagagtg cccttcagag
     1141 atgaagaagg actgatgagt ctgattatca tgcggggaat ctgccaatgt ccccacatag
     1201 acttcagctc gtcccgcggt gatgatgccg gaactgatac ctcgttgcaa ttgggaagcg
     1261 aagatttcga gaaaaaggga tgttgaatcc tctcagtgaa aaaggagact attggccacc
     1321 ttccggtgga gaaccttcat tcgttgtccg ggtgctgatc aggtatgcga tcactcccac
     1381 gaccacgatg atgagcaggc ttgccagggc gataatgccc agggggagtc ccggggatcc
     1441 ggtagatacg ggaggaactg gtggttccgt ggtgggcagg ggttgtgcac cctcatcgag
     1501 acagagtaca taggtgcccc ctcttgttac agccgccttc atcgtccgtg aaccgggatc
     1561 catactggta gcaatctcct cccatgatgc gtcggttgta ttataccgtg ccagtgaagc
     1621 tccctccttg tccagacctg cccatgtggt ctcgttaaag gagagcacaa gggtgactcc
     1681 ggaagtaaac ctcaccccgg caggtgtaat ctcgtattga tagggggcag caatgcaggg
     1741 ggatttcgga gggattgtac cgggaggcag agcggagaga gagacggttg agagcggagc
     1801 tccggtggaa tcgacaggag ccaccccctt accgattgcc agtgacccga tggtgtcggt
     1861 agctgcaatg gtaacctctc ttgtggtggt attgtcggtg ccgaggagca ggtttcctgc
     1921 actcatccgg gtaaccgtcg ggctaggggt cagggttccg gtcatggtgg tggtggcagt
     1981 ctgaccgctg gtgatgacgg ttccactacc gcctccgcct ccaccgccgc ccccgccccc
     2041 gcctccgctg gggctgccct gggcaaacac ggtgatataa ttgctctttg tgcggacatt
     2101 ggtcagcgtt ccgctgatga ccgtgaggga taccgtgtat gtaccggcag ttgtgtaggt
     2161 atggctcggg ttctgttcgg cagaggtctt cccgtcaccg aaattccata accattgatc
     2221 atgggatcca gtgctctgat cagcgaactg cgccgtatgc ggggcatacc cgtccggccg
     2281 attcaccgaa aaatcagcca ggagggccac ctccccggcg ttcaattcaa ggttggtcac
     2341 cgcacccggg gtgaaggggt aactctcctg ccatccctct ccgctcccgt actcacgaca
     2401 gagtgccggc gatccctcaa tcgcgaacgt gatcctggaa gtggatgcct ggaaatctcc
     2461 ctggacgatc agcttctggt ccagtgcacc ttctcccccg aaggttcctg caggggatac
     2521 gctgatcatc ccattcgccg caccgtcaac cgtcgtggtg atcgtatatc cttcgggtgc
     2581 cgggcttccc atgatcgttg ccgtcccgta gaacatatgg gggaactgag gctgggtgtt
     2641 gctctctccg ctacagatac cacaaaaagc gagagccaga aagaggatga ccatcatgca
     2701 ttgccccgtt ctgccaatcc tcctggtttg ttcatgcctt cctatcggaa tatgagcgtc
     2761 attattccga tgttgtccct ggttacttct ctcctgcacc atgctctttc aacctctgct
     2821 ggccggattg caaacccggc aggtcagggg atctctttcc cgggggatgc caatccttcc
     2881 acgcacgtaa tgtttatata taacggaatg atggcgttca tacataagtt ttttggaaga
     2941 atctcccatt ttctggaata aaatcaaaaa agaactcgtc caatccatta cctgatcatg
     3001 attccgggat ccggctgctc tcagtagttc tgaaaagaaa taccatgatt cctttgaggg
     3061 ccacaagcat aaaagatgaa aatgccggag gcattttc
//
LOCUS       DAOOCO010000202         3087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_30964_length_3087_cov_4.276055, whole genome shotgun sequence.
ACCESSION   DAOOCO010000202 DAOOCO010000000
VERSION     DAOOCO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_3087_cov_4.276055"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PK154_09530"
     CDS             complement(<1..1281)
                     /locus_tag="PK154_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11339.1"
                     /translation="MIAKKTMIQELGEDELILPTLVNNALAANDRIKYFFTLLQAARL
                     HAMHPGRETSNLKAEREAAGVDDPMFDRVVTDSVIVGKDTLQIPYAPLILSGIRSSLA
                     EMAAPLPVYDEGKGEFFQKRVALLAEGIPSGENNLIADEVIQRITAGDEGTIDSIHLL
                     VMDIHKELIVLQGDLASEVIDGAMAYLLHDEDRDLVAAFMAGLKRTSPLRFDHPGLGT
                     TATRSRGTLIIQNDIGMTDAHVLVVNVEEPTVTVIYTDIHMPRLQFFQALFDEQGMTW
                     EDTLSKNSGDQFEAKIYHLSVGTYRAGSREDLERFLKFLGSRLVFLIDWNRARKRLRT
                     FLQNRDASAVLKWAADNEYGHMAFLLLGGEKIIYEALELASRVPLRYGEPLHQILGRE
                     RTVEYFKWVLKTASTGLRAGHSRLLLQDEIKAELL"
     gene            complement(1435..2445)
                     /locus_tag="PK154_09535"
     CDS             complement(1435..2445)
                     /locus_tag="PK154_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPW11340.1"
                     /translation="MEIRKVQITGGSSYVITLPKDWAKGQNIQKNDPLGLLSQPDGTL
                     LVTRNITEGQFQREKVFSLADINEPVYLFRLLVGSYISGFTGIRITSKTRIPVSMRMV
                     AREFTQMAIGFEVVEETDMTITLKDLLNPAEMPFENTIKRMFVIVKNMQIDAMSALEN
                     GGDPGIADDVIKRDNDVDRLNWLIARQTNMILKNAQLSRKMGAPTSKVVNTYIISRII
                     ERIGDHAVRIAENVKKISGSQINKDVLTSIMKANNLAISLFDKSIVSFFNSDLKGSNK
                     NIEQVEKLEELYEKVALLAMKEEPSLAIPLRSIAESIRRSGEYAGDISESIINYLIEE
                     KP"
     gene            2660..>3087
                     /locus_tag="PK154_09540"
     CDS             2660..>3087
                     /locus_tag="PK154_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW11341.1"
                     /translation="MSLKHLIMPPDKVYFDLLERQAANVTEAARQLLNLTKNFTSIKE
                     KRLEIEVLEHTGDEITHEIYERLNKRIRPPFDPEDISKLASALDEVLDYVDGTIERML
                     YYGIQSSDSYMIELAKLIHLSTIEIEAAVKSMRSMKDPRY"
BASE COUNT          777 a          767 c          709 g          834 t
ORIGIN      
        1 gaggagctct gccttaattt cgtcctggag cagcagccgc gaatgtccgg cacgtaaacc
       61 cgtcgaggca gtcttgagca cccacttgaa atactcaaca gtccgttccc tgccaagtat
      121 ctggtggagc ggttccccgt atcgcagggg aacccttgag gcaagctcaa gggcttcgta
      181 aataatcttc tcccccccaa ggaggaggaa cgccatatgg ccgtattcgt tatctgctgc
      241 ccatttgagg acagctgagg catctctgtt ctggaggaat gtacggagac gctttcttgc
      301 acggttccag tcaatcagaa ataccagcct cgaaccaaga aatttcagaa acctctccag
      361 atcctccctg ctcccagccc ggtatgttcc aaccgacaga tggtagatct tcgcctcaaa
      421 ctggtcacca gagttctttg agagagtatc ctcccacgtc atcccctgtt catcaaacag
      481 cgcctggaag aactggaggc ggggcatatg gatatcggtg tagatcaccg ttaccgtggg
      541 ttcttccaca ttcaccacaa ggacatgagc atcggtcatc ccgatatcat tctggataat
      601 aagtgttccc ctgcttctgg tagccgtggt accaagcccg gggtgatcga atcttaatgg
      661 agaggttctt ttcaggccgg ccataaacgc cgctacaaga tctcgatctt catcgtgaag
      721 gaggtacgcc atcgccccgt caataacttc actcgcgaga tctccctgaa gaacgattaa
      781 ctctttgtgg atgtccatta caagcaggtg gatactgtcg atcgttcctt catcacccgc
      841 ggtaattctc tggataacct catcagcgat gagattgttc tctccggaag ggatgccttc
      901 agcaaggagt gccacccttt tttgaaagaa ttcgcccttc ccttcgtcat atacagggag
      961 aggggctgcc atctccgcaa gggacgagcg gatacctgac aggatcaggg gagcgtaagg
     1021 gatctgcaga gtatccttcc ctacgatcac actatcggta acaactcgat cgaacatcgg
     1081 gtcatccact ccggctgcct ctcgttctgc tttcagattc gaggtctccc ttccaggatg
     1141 catggcatgg agacgggctg cctgcagcag cgtaaagaaa tatttgatgc ggtcatttgc
     1201 cgcaagggca ttatttacca gtgtcggcag gatgagttca tcctccccaa gctcctggat
     1261 catcgtcttt ttggcgatca tggcggtagt atcccgagac tgggtgatgc ctgcaggtat
     1321 cgccgaatct ctcctgaagg taaaataggg gagcggatat aaagaatttg ttatgattga
     1381 aggcaaaagg caggatatcg agtaaaggag agagatagaa gaaatgtcca attctcatgg
     1441 cttttcttcg atgagataat tgattatgct ctctgatatg tcacccgcgt attccccaga
     1501 tctccgtata ctctctgcta tagaacgaag gggaatagcg agggagggct cttctttcat
     1561 ggcaagcaaa gcgacttttt cgtagagctc ctcgagtttc tcaacctgtt cgatattctt
     1621 atttgatccc ttgagatcac tgttgaagaa cgaaacaata ctcttgtcaa aaagggagat
     1681 ggcaagattg tttgccttca tgatggatgt cagtacgtcc ttattgatct gactgcctga
     1741 aattttctta acattctccg caatccttac tgcatgatca ccaatccgct cgatgatacg
     1801 gctgatgatg taggtattca ctaccttact ggtcggagcc cccattttgc gggagagctg
     1861 tgcatttttc aggatcatgt ttgtctgccg ggcgattaac cagttgaggc gatcaacatc
     1921 gttgtcacgc ttgataacat cgtcagcaat tcctgggtct cccccgtttt caagcgctga
     1981 catcgcatca atctgcatat tcttgacgat gacaaacatc ctcttgatcg tattttcgaa
     2041 gggcatttct gctggattga ggaggtcctt cagagtaatc gtcatatccg tctcttcaac
     2101 tacctcaaac ccgattgcca tctgggtaaa ttcccgtgcg accattcgca ttgaaacagg
     2161 aatccttgtc tttgaggtaa tacggatgcc agtaaatccg gaaatataag atccaaccaa
     2221 taatctgaac agatacaccg gctcattgat atctgcaaga gagaagactt tctcccgctg
     2281 gaattgacct tctgtaatgt ttcgggtaac aaggagtgtg ccatccggct gggagagtaa
     2341 tcccaatggg tcattttttt ggatattctg ccctttagcc cagtctttcg gaagcgtgat
     2401 gacataggat gacccacccg ttatctgtac ttttctgatt tccatgagtg ccccacattc
     2461 tcttgatatc tgtatttctc aataaaatca atacacatct atagatttct ttttaaccca
     2521 aggggaccga tcaaaaaggt ggggatatat catccagatt ttgccagtat atgagaagat
     2581 ctctcataag cgggtgtcat tttcactact ggaccatcat ttttttatgt tgccatatcc
     2641 atacacatgt ggtttcctta tgagcctcaa gcacttgatc atgcctcctg acaaggtgta
     2701 tttcgatctg ctcgaacgcc aggcagcaaa tgtcacagag gcagcccgcc agcttcttaa
     2761 ccttaccaag aattttacca gtatcaagga gaaacgtctg gagatcgaag tacttgaaca
     2821 caccggtgat gagatcacgc acgagatcta tgagcggctc aacaagagga ttcgcccgcc
     2881 ttttgatcct gaagacatat ccaagcttgc atctgctctc gatgaggtgc ttgattatgt
     2941 cgatggaacc atcgaacgga tgttatacta tgggattcag tcctcggata gctacatgat
     3001 tgaacttgcc aaactcattc acctttcaac cattgagatc gaggcagccg tgaaatcaat
     3061 gagatccatg aaagatccac gatatat
//
LOCUS       DAOOCO010000203         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_31291_length_3064_cov_4.073446, whole genome shotgun sequence.
ACCESSION   DAOOCO010000203 DAOOCO010000000
VERSION     DAOOCO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31291_length_3064_cov_4.073446"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..2119)
                     /gene="metG"
                     /locus_tag="PK154_09545"
     CDS             complement(128..2119)
                     /gene="metG"
                     /locus_tag="PK154_09545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW11342.1"
                     /translation="MSTRPVLVTCGLPYTNGPCHLGHLRTYVPADFYVRYLRRTGEEV
                     VFICGSDNHGTPIVVSAEDAGISPRALSEQYHAHFDQTFRRMNVVFDHFGMTDDPTNH
                     RRTRSIVERLIANGHVYSKVIQQSYCTKCLRFLPDRYVEGICPHCGAPARGDECDQGC
                     GKHLEPGEIKKPICTVCGTRAESRAQEHFFFRLSSFKDYLLEHLETLSGTLNARNYAL
                     GWVRDDLHDWCITRTLDWGVKFPGRDDLVVYVWVDAPIGYISFTEEWAEKNGKSWEKY
                     WCGDNARVIHFIGGDIIYHHCIFWPALLKGAGYGVPHAVVASGMLKVDDHKFSKSRGY
                     VVWTNDDYLDKGLPADYLRYYLLAYTNHTKELNFSWKMFQERINNELVNTLGNFAYRT
                     LFFARKEFGGIPDTAVQPDILKEIGSTLFQVDSTIRDYEFKTAIDAMMTLAAYGNNYI
                     QTNAPWKLIKTDRNAAAQAVKDCIVLVRALALLFEPVIPGKAQELWEMLGNTDRIANH
                     RINEAIQDVSAGPLPIPSPVFSKLDDDFIGEMDKLLTYRIAEAEKKMVKTVTVPFEEF
                     CKLDIRTGKVISAEGVPKSNKLLKLKVDIGGETRQIVAGMAQFYTPEEMVGKSVVVVV
                     NLQPARIFGIESNGMILAAGDTASLLTPEKPVQPGTKIR"
     gene            2305..2955
                     /locus_tag="PK154_09550"
     CDS             2305..2955
                     /locus_tag="PK154_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11343.1"
                     /translation="MPQIEAGINTPCAGGKFYQDRLINSFIGTEGRVITGEIGYDSFP
                     LVKDAEYLSAIQKDLWFAFPSPGELRLNNRYYKDTDEVLPALVSVYHAMMRSMRDRGI
                     FGHILHCDTPDKEELEALAGQKVFFFSHRETKKNLGLILEYQDILAVRSSALGVVAEI
                     MDDYDIQKIILVDAREEDLLRALEFRDAEHLICGGYCQDSCVHYWKSVVENASVFR"
BASE COUNT          779 a          778 c          735 g          772 t
ORIGIN      
        1 cacttacaca tggaaatcag caagtcagcg gatacgtgtg ccatgacaat ccctggccct
       61 accatcaatt tttcatccat cttatgaatt tgaagaaatg aataagaaat gaattcctgg
      121 aaaaaaatta tctgatcttg gtacccggtt gcacaggttt ttcaggtgtt agaagtgatg
      181 cagtatcccc ggcggcaagg atcatgccat tgctctcaat gccaaaaatc ctggcaggct
      241 ggaggttcac aaccaccacc acgctctttc cgaccatctc ttcaggggta tagaactggg
      301 ccatcccggc aacgatctgt cgtgtctccc ccccgatatc caccttgagt ttcaggagtt
      361 tatttgattt cggcaccccc tctgcggaga ttacttttcc cgtcctgata tccagtttac
      421 agaactcttc gaaaggaacc gtcactgttt ttaccatctt cttctctgcc tccgctatcc
      481 gatatgtgag aagcttatcc atttcaccaa tgaaatcatc atcgagcttg gaaaataccg
      541 gggatggtat agggagtggt ccggccgaaa catcctggat cgcctcattg atccgatgat
      601 tagctattcg gtccgtattc ccgagcatct cccaaagctc ctgtgccttc ccaggtatta
      661 ccggttcaaa gagaagcgcc agcgccctta ccagcacaat acagtctttc acggcctgcg
      721 cagcagcatt tcgatccgtc ttgatcagtt tccagggggc attggtctgg atgtagttgt
      781 tgccgtaagc agcaagggtc atcatcgcat caatggccgt cttgaactcg tagtccctga
      841 tcgtggagtc cacctggaac agagtactcc cgatctcctt aagaatatca ggctgtacag
      901 cggtatccgg tataccccca aactccttcc gggcaaaaaa taacgtccgg tacgcaaagt
      961 tcccgagtgt gttgacaagc tcgttattga tacgctcctg gaacatcttc caggaaaagt
     1021 tcagttcctt ggtatgattg gtatacgcaa gcaggtaata cctcaggtag tccgcaggaa
     1081 gacccttgtc aaggtaatca tcgtttgtcc atactacgta tccacgcgat tttgagaact
     1141 tgtgatcatc caccttgagc attcctgaag ccaccactgc atgggggacc ccgtatcctg
     1201 cacccttcaa gagtgccggc cagaagatac aatggtgata aatgatgtcc cctccaataa
     1261 aatggatgac ccgggcattg tccccgcacc agtacttctc ccacgactta ccattcttct
     1321 ctgcccattc ctcagtgaaa gagatgtatc caatgggggc atcaacccag acatacacca
     1381 ccagatcatc ccgccccggg aatttgactc cccagtcaag ggttctcgtt atgcaccagt
     1441 catgcaggtc atctctcacc cacccaaggg catagttccg ggcattaagg gtccctgata
     1501 acgtttcaag atgttcaaga aggtaatcct taaaactgct caacctgaaa aaaaagtgct
     1561 cctgggccct cgattctgcc cgggtaccac atactgtgca gatcggcttc ttaatctcgc
     1621 ctggttcaag gtgtttcccg catccctggt cacattcatc tcctctggca ggtgccccgc
     1681 aatgtgggca gattccttca acataccggt cagggagaaa ccgcagacat tttgtgcaat
     1741 agctctgctg gatgactttt gagtacacgt gcccgttagc aatgaggcgc tcaacgatcg
     1801 acctggttcg acggtgattg gtgggatcat ctgtcatacc aaaatggtcg aataccacat
     1861 tcattctgcg gaacgtctgg tcaaaatgag catgatactg ttcactcaat gccctgggtg
     1921 agattcctgc gtcttcggca ctcaccacaa tgggcgttcc atgattatcg gacccgcaga
     1981 tgaaaacaac ctcctcacca gtccggcgta aatagcggac ataaaagtca gccggtacat
     2041 aagtacggag gtggccaagg tggcaggggc cattggtata agggagcccg caggtcacga
     2101 ggacgggtct tgtactcatc attatctcgg tttgacgatg attctataaa taacgtgatt
     2161 actatgcgaa agcgagtctc atttctgacc aggtcattag gttcggatgc acccatccct
     2221 gatcgcgaag tcttggcaga atgggtgaga gcacggcggg gacgagaggc ggacctcatc
     2281 acgtttcagc ttgagggagg tttgatgccc cagattgagg ccgggatcaa taccccctgt
     2341 gcgggtggaa agttctatca ggaccgttta atcaactcct ttatcgggac tgagggaagg
     2401 gtaataacag gagagatcgg atacgacagc tttccccttg tgaaagatgc cgaatacctt
     2461 tcagccatac aaaaagatct ttggtttgct ttcccatctc ccggggaact caggctgaat
     2521 aatcgatatt ataaagacac tgatgaggta ctgccggctc ttgtctctgt atatcatgca
     2581 atgatgcgat cgatgcggga tagggggatt ttcggccata ttctccactg tgataccccg
     2641 gacaaagagg agttggaagc gcttgcaggt cagaaagtct tttttttcag ccacagggaa
     2701 acaaaaaaaa atctggggct gatccttgaa tatcaggata tcctggctgt caggtcatca
     2761 gcacttgggg ttgttgcaga aatcatggat gattatgaca tccagaagat tatcctggtc
     2821 gatgctagag aggaggatct ccttcgggca ctcgaattca gagatgcaga acatctgata
     2881 tgtggagggt actgccagga ctcatgtgtt cattactgga aatcggtagt ggaaaatgcc
     2941 tcggtgttca gatagattcg gcgatatcca cgaaacgcct gaattgagag ctgctgccaa
     3001 gaagtatgaa ccgaattctt tcaaggaatg aaaattgaaa cgatccacca gcagcgttcg
     3061 ggtg
//
LOCUS       DAOOCO010000204         3032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_31757_length_3032_cov_4.673833, whole genome shotgun sequence.
ACCESSION   DAOOCO010000204 DAOOCO010000000
VERSION     DAOOCO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31757_length_3032_cov_4.673833"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..814)
                     /gene="msrA"
                     /locus_tag="PK154_09555"
     CDS             complement(335..814)
                     /gene="msrA"
                     /locus_tag="PK154_09555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPW11344.1"
                     /translation="MDDSKQSEVATFAAGCFWDVEAEFRMREGVVATRVGYTGGDVSD
                     PTYEQVSSGDTGHVEAVQVVFDPARIPYRELLTLFWGLLDRSSGMDENQRPVIFYHSP
                     EQEAAAKDFLKRVMRTPSVPVIVEVLPAGTFWEAEESHQHYYEKCGQGYGTSTKFWG"
     gene            962..1873
                     /locus_tag="PK154_09560"
     CDS             962..1873
                     /locus_tag="PK154_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPW11345.1"
                     /translation="MPPCTLVLNDRSVHHDLKGHPESELRMEQIRSSLQGNRRSLQAS
                     PALMDDLLRVHMPEYLSRLEHLSGRCRPGGVAFLDSDTYVVSGSWDAARYAAGASIAA
                     AGEALSGRPAFAVVRPPGHHAGPVYGMGFCLLNNVAVAAAWSLSRVSQVAIIDWDVHH
                     GNGTQAMFSDSDRVLYCSVHRSPFYPGTGSREETGSGAGEGCTVNVPLPAGSGSEAYL
                     RAFQEEILPAVCSFDPDLILISAGQDPLADDPLGGMNLEPGDFGVLTELVCSAARKPP
                     ALVLEGGYGPSHGEAVMEILRVLDQWE"
     gene            complement(2082..>3032)
                     /locus_tag="PK154_09565"
     CDS             complement(2082..>3032)
                     /locus_tag="PK154_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW11346.1"
                     /translation="PEYGSNGEDLSFKILDLAVHPEKYYITENENIKKDGSRVWIAWT
                     NQPLFDDKGTIVEILSIGNDITRLQEAEREIQELNNELENRVIERTRQLTEVNKNLES
                     FTYTISHDLRAPLRAISGYSSLLMQDLKDIPEKDKRYLESLRQNAHDMGRLIDDLLEF
                     SRLRIRSLEKETVQPSVMVREILREIRKDPAFAKVEFNVGELPPCQADPGLLKQVYAN
                     LIGNAVKYSQKRAAPLVEIGSLTRDGQLMYFVRDNGIGFDMKYSEKIFGVFERLQTSD
                     EYEGTGIGLSIVHRIVEMHGGRIWVESEVDKGTTFYFSCW"
BASE COUNT          614 a          873 c          797 g          748 t
ORIGIN      
        1 ctggtcttct cggaaaacat cggggcttat tcgattgcat catccaccta tttcaacggg
       61 tttcccccgg caaaggtggt aaatatcaac cagtgatcct tttttcacat cttttttttc
      121 gatataaacc ggtattttca tagttgatga tctaccggac ggcgactcac acatgggaaa
      181 aggaaaaatg cctccttcat tttcatttat atgcgtgtgg ccttctatgg catcgggtaa
      241 tttcttcagg gtcctgatgg gtaccgatgt ggctatcgct tcggtgggca tcggggtgag
      301 ggccacacgc tgtcgaagag tggcttgagc gaggtcatcc ccagaacttg gttgatgttc
      361 catatccctg tccgcacttt tcataatagt gctggtggct ttcttctgcc tcccagaacg
      421 ttcctgcggg gagtacctca acaataacag ggacactcgg agttctcata accctcttta
      481 aaaaatcctt tgctgctgcc tcctgttctg gtgagtgata aaaaatgaca ggccgctggt
      541 tctcatccat cccagatgac ctgtcaagga ggccccagaa gagggtgagc agctcgcgat
      601 acgggatacg ggcaggatca aacaccacct ggacggcctc gacatgccct gtatccccgg
      661 aggagacctg ttcataggtg ggatcggaaa catctccccc ggtatacccc actctggtcg
      721 caacaacgcc ctccctcatc ctgaactctg cctcgacatc ccagaaacac ccggcagcaa
      781 acgttgccac ctcactctgc ttgctgtcat ccatacccgt atatttcgcg ccgggaacga
      841 tggacattgc ggtgtcgggg agtctcgacc aaaccggggg cacgtttgtc ctcctcccgc
      901 ccaggagccc ggaccggcgc cgatgcttta tatatcattt ccctgtatct tcctgtacgc
      961 catgcccccc tgcaccctgg tcctgaatga tcgctcggtc caccatgatc tgaaaggtca
     1021 tcccgagtca gaactgcgga tggagcagat ccgatcttct ctccagggca accggcgatc
     1081 cctccaggct tctcctgccc tgatggatga tctcctccgg gtgcatatgc cggaatacct
     1141 ttcccggctc gaacacctat caggccgatg ccgtccaggc ggtgtcgcct tcctggattc
     1201 ggacacctac gttgtctctg gttcctggga cgctgcacga tacgcagccg gtgcatcgat
     1261 cgcagctgca ggagaggcac tctcgggccg gccggcattc gccgtggtcc gtcctcccgg
     1321 ccaccatgca ggcccggtgt acgggatggg attctgcctc ctcaacaacg tagctgttgc
     1381 cgctgcctgg tctctttcgc gggtatccca ggttgccatc atcgactggg atgtccacca
     1441 tgggaacggg acccaggcga tgttctctga ctcggaccgg gtactctact gctccgtgca
     1501 ccgctcaccc ttctaccctg gcaccgggag cagggaagag acaggaagcg gggcagggga
     1561 aggatgcacg gtaaatgtcc cgctccctgc cggatcaggg agtgaagcat atctccgggc
     1621 tttccaggag gagatcctcc ctgctgtctg ctcatttgac cctgacctga tcctgatctc
     1681 ggcagggcag gatccgcttg ccgacgaccc gcttggggga atgaatctgg agccgggtga
     1741 ctttggcgtg ctcacagaac tggtatgctc tgctgccagg aagccccctg ctctcgttct
     1801 tgagggaggg tatgggcctt cccatgggga ggctgtcatg gagatccttc gggtccttga
     1861 tcagtgggaa tagcaccgca tcgatgctga gccatgtggg gaggagcagc ggggctctca
     1921 ccacgagggc ggtagtatgg ttctccatga ctcgatttca gttgagagtg gaatgaaccg
     1981 cgagggtcag ggcattcgca ggtgatagtg tgatcaggaa agaaacagta cgatcgccaa
     2041 tctcctctta ttcgtattat tccacggcca tcgtgtgttc gctaccagca ggagaaatag
     2101 aacgttgtgc ccttgtccac ctcggattct acccagatgc ggcccccgtg catctccaca
     2161 atccgatgga caatcgaaag gccaatcccc gtgccctcat actcatcaga agtctgcagc
     2221 ctctcgaata ccccgaagat cttttctgag tatttcatat caaacccgat cccgttatcc
     2281 ctgacgaagt acataagctg cccgtcccgt gtcagggagc cgatctccac aagcggagct
     2341 gccctcttct gtgaatactt cactgcgttc ccgatgaggt ttgcatacac ttgttttaac
     2401 aaccccggat ctgcctggca gggggggagc tcgcccacgt taaattccac ctttgcgaac
     2461 gcggggtctt ttctgatctc gcggaggatc tctcttacca tgacggaagg ctgaacggtc
     2521 tccttctcca gcgatcgaat ccgtagtcgt gaaaattcca ggagatcatc gatcagcctg
     2581 cccatatcgt gggcattctg ccggagcgat tcgaggtatc ttttatcttt ttccgggata
     2641 tccttcagat cctgcatgag gagggaggaa tacccggaga tggccctcaa aggagcacgg
     2701 agatcatggg agatggtata ggtgaaggat tcgagattct tgttgacctc agtcagttgc
     2761 cgggtccgtt cgattaccct gttctcgagc tcgttgttga gctcctgtat ctcccgctcg
     2821 gcctcttgta accgggtgat atcattccct attgacaaaa tctcaacaat cgtacctttg
     2881 tcgtcgaaca aaggctggtt tgtccaggca atccataccc ggctgccatc cttcttgata
     2941 ttttcattct cggtgatgta gtatttttcc ggatggactg caagatcgag gatcttgaag
     3001 gagagatcct ctccgttact cccgtattcc gg
//
LOCUS       DAOOCO010000205         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_32666_length_2977_cov_5.221081, whole genome shotgun sequence.
ACCESSION   DAOOCO010000205 DAOOCO010000000
VERSION     DAOOCO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32666_length_2977_cov_5.221081"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(633..1784)
                     /locus_tag="PK154_09570"
     CDS             complement(633..1784)
                     /locus_tag="PK154_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPW11347.1"
                     /translation="MHSIDAALASNRINKRDADLIKEFIAERKVAANIGASRANKFIY
                     VLVGWRRFVGPYHELSIAEVYTSIDTMKNSLTQKGTPFKQNTKHDWVRILRQFLLWMI
                     ENEYSNLPERKIRRIQVPTKDTMTHKAADLLTPEEVKALLAACRWSRDRALIGMLYEG
                     GFRIGELGQMKWSDLSFDSTGVVANVNFKTGIPRYVRIIMAKGYLSQWRADYPGTPEG
                     NALVFLNELRRPITNGGVIKQLRRICERAGITKHVTPHLFRHSRITHLINEGVSESVI
                     KMIMWGSVHTTMFKVYAHLTGNDIDEELRRVYGIQKEAEKKRVPRLEPRVCPNCHSLM
                     PPVADYCAHCGESLLKSRTAREEDIQKFVKNHKAELMEYLDRTPATVQE"
     gene            complement(1931..2004)
                     /locus_tag="PK154_09575"
     tRNA            complement(1931..2004)
                     /locus_tag="PK154_09575"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1968..1970),aa:Phe,seq:gaa)
     gene            2091..>2977
                     /locus_tag="PK154_09580"
     CDS             2091..>2977
                     /locus_tag="PK154_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HPW11348.1"
                     /translation="MVAYLGFAGKGGAGGGFEERVTNLLKSRRSTLVHLTHNDLDAVG
                     SDAIHRMRYGTITSIFCSVGTFPQALDALASAEGKGTILSISDLGYQKGIEHRIRSVS
                     EAGWRIQWRDHHRWSADEREQIQEFCDLLMLDTSTCGCGICARDLLPGDETAAEIARV
                     VCDYDLWKHEDPRSAMLGVVLQRKKNRDYVRDRLAEGILTDEYIEREYREIVGDMEEK
                     MAKSIRKATLMGEKYRIAFSPLFGYPSETAARMRKELSTDIEVLISKDGRFSLRTVPP
                     ISHIIARHYGGGGHPNAAGG"
BASE COUNT          718 a          736 c          823 g          700 t
ORIGIN      
        1 gccattaccg atttcgatga ggagaaagat aaacagagtg cagccatcat catctaatag
       61 aaaggagatt tcgcctcatt ttttttagtt ttaccattct gccgcagggt catttcacta
      121 taaaaacagc cttacacctt ttcacccgca tttctgcgcg agcacaaaaa taactgattt
      181 aagggctccg ttgccaaccc ggctggggct tcagaacccc atggttaaat tccttaacga
      241 gaggagtatc cttcggccgc agcggcccga gcgagcgccg gttaccgagt gcatcagcac
      301 gaggtcgagc gctgcgagtc actgagcgtt agtatgtgta cggtactcag cgacgagcac
      361 gaagcgaagc atggtgcgag gagcgggatg taagcagctg ccggtgagga gtgaaacgac
      421 gaacccggga gcgtcatctt atcacaatta accgtgggaa aacaggccca tggtgtgagc
      481 ggaatggagt ggagtgagca ccaaccggag cggaacgaaa cgaaatggag acaagcacga
      541 tggggcgcca tctctctcct ggacctggtg gtcccgcccc ttcggattgg tggggcgggc
      601 accgggaggg gcgatccgga agagatagaa aattattcct gcacagtcgc cggtgttctg
      661 tccagatatt ccatgagctc cgctttgtgg tttttcacga acttttggat atcctcttcc
      721 ctggccgtac ggctttttaa gagagactct ccacaatggg cgcaatagtc agcgaccggt
      781 ggcatgagcg agtggcaatt agggcagacc cggggctcga gccgaggaac acgttttttc
      841 tctgcttcct tctggatacc ataaacccgg cgcagctcct catcaatgtc gttccccgtt
      901 aagtgggcat aaaccttgaa catcgttgta tgcaccgatc cccacataat catcttaatt
      961 acactctccg agaccccttc gttgatgaga tgagtaatcc gcgaatgtcg gaagagatgc
     1021 ggggtgacat gcttcgtaat tccagcgcgc tcgcatattc tgcggagttg tttgatgacc
     1081 ccgccattcg tgattggtcg cctcaactca ttcaggaaga ctaatgcatt tccctctggg
     1141 gtaccgggat aatcagcccg ccactgggag agatatccct tcgccatgat gatccggaca
     1201 tatcggggaa tcccggtctt gaagttcaca ttggcgacca cgccggtgct atcaaaggag
     1261 agatcgctcc acttcatctg gccaagctca ccgatccgga agcctccctc atagagcatt
     1321 ccaataaggg cccggtcccg gctccagcgg caggccgcca ggagtgcctt tacctcttcg
     1381 ggtgtcagga ggtccgcagc cttgtgggtc atcgtatcct tggtagggac ctggattcga
     1441 cggattttcc gctccgggag attggaatac tcattttcga tcatccagag aaggaattgc
     1501 cggaggatcc gaacccagtc gtgcttggta ttctgcttga agggagtgcc tttctgggtg
     1561 aggctattct tcatcgtgtc gatgctggta tatacctcgg cgatcgaaag ctcgtggtaa
     1621 ggcccaacaa accgtctcca tccgaccagg acatagatga atttatttgc acggctggct
     1681 ccaatgttgg cagcgacctt tcgctcggct atgaactcct tgatgagatc ggcatcccgt
     1741 ttgttgatcc gattcgatgc caatgcagca tcgatcgaat gcatgccata ctcagctttg
     1801 atacaggaga aacggtgtga agatgctact tcagtccgta cggtagtggc tatcgtggaa
     1861 gataccatag aaacgggtct gaaaggatat acaaaaactt tgcgaccagg atttgaacct
     1921 ggcgtccgga tgcctcagcc cggatttgaa ccggggacaa ccagatcttc agtctggcgc
     1981 tctcccgaac tgagctactg aggcgaggaa tacattattt agctctcagc ctaataaatt
     2041 gtattattta tgggtgcata tcccaaaaaa tgcaccgatt tttacctttg gtggtggcgt
     2101 atctgggttt cgcaggaaaa ggcggtgctg gcggtggatt tgaggagcgg gtaaccaatc
     2161 tgctcaaatc aaggagatca accctcgtgc acctgactca taacgatctt gatgccgtag
     2221 ggagcgatgc aattcacaga atgcggtacg ggacgatcac gagtatcttc tgctcggtcg
     2281 ggacctttcc ccaggcgctc gatgcgttag cttctgcaga ggggaagggg acaattctct
     2341 ccatcagcga tcttgggtat caaaaaggga ttgaacatcg tatccggagc gtatctgaag
     2401 caggctggcg gatacaatgg cgtgatcatc atcggtggtc cgctgatgag agagaacaaa
     2461 tacaagagtt ctgtgatctt ctcatgctgg acacatcgac ctgtgggtgt ggtatctgtg
     2521 ccagagatct gcttcctggt gatgaaacag cagcagagat tgcccgggtt gtctgcgatt
     2581 atgacctctg gaagcatgaa gatccccgat cagcgatgct gggtgttgtc ctgcaacgga
     2641 aaaagaacag ggattatgtc cgtgaccgtc tggcagaagg cattctcact gatgagtata
     2701 ttgaaaggga ataccgggaa atcgtgggag acatggagga gaaaatggca aagagcatcc
     2761 gaaaagccac actcatggga gagaaatatc gtatcgcatt ctctccgctt tttggatacc
     2821 ccagtgagac agccgcccgg atgaggaaag aattatcgac cgatatcgaa gtgctcattt
     2881 ctaaggatgg ccggttctct ctccgaacag ttccgccaat aagccatatc atcgcgcgtc
     2941 attacggggg aggggggcac ccgaacgctg ctggtgg
//
LOCUS       DAOOCO010000206         2947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_33118_length_2947_cov_6.173237, whole genome shotgun sequence.
ACCESSION   DAOOCO010000206 DAOOCO010000000
VERSION     DAOOCO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33118_length_2947_cov_6.173237"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK154_09585"
     CDS             complement(<1..286)
                     /locus_tag="PK154_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW11349.1"
                     /translation="MILDQEKITRIKKLLKARPKGLTISDISHNLKINRNSVAKYLEI
                     LLITGQVEMRMYGNAKVYYLSHRVPISSMLKFANELILVLDSEFKVVETNE"
     gene            453..>2947
                     /locus_tag="PK154_09590"
     CDS             453..>2947
                     /locus_tag="PK154_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW11350.1"
                     /translation="MTTPGKKRSFSFYLLVFLLIIISVIVAVITIGDLVLVNENFQDH
                     AELLRNETEQNLIESVATIDNGLKLFDNTLNKRMEQGFVIFHRGYREAGGNPSRMNLA
                     YLKKEMGGEMDLYIINGSGVVEYTTYTPELGLNFKEMIPYFYEYLMEIKAKPGFYPDR
                     VVQESATGNLKKFAYSPTEDHEYILELGLSAESFKSERNTLKYTDMIESVRQRSPYIS
                     DLRIFTTAKRQVGNKSFKPDAEMNAILDQVLATRQGLVIPDNDPRLTTKYLFIDLKDE
                     DYAADMSLIVEIIYDDALIFQSQQQLIAFHLLVAGFGLILGIIGAFSLSRYLTRPIAQ
                     IAADTDIIAHGDLDHRIASTKGSEFGILERSINALVLSLKGMIQQLKHSESKLRESEE
                     RYRGVVESQSEFITRFRPDGTITFANEAYCRYFGLPDCDITGKRFIPAIPDEERKRLD
                     EGFRNLTPENPELSIEHRIILANGESRWHQWNDRAIFSPEGNVIEYQSIGRDITEQRQ
                     IEKDLIESERRFRDLATLLPQVIFEVDLNGRITYVNESAYQIFGYNPGELEKGITIFQ
                     IIVPEEHEQALRNLEESIEGKKTEGAEYNMVRKDGSNLNAMVYSSPIMSEGAVAGIRG
                     ILVDITQLKKVEDDIRRLNEELEMRVTERTRDLEMANRELDSFSYSVSHDLRAPLRAI
                     DGFSMILLQEYGKNLPKEIVSYLEKIRENTGRMGVLIDDLLNFSRMSRLPLNRFLVDP
                     SSIVREAYEEFRLETTGRQIDLMIKEMPTCSADPALLNHVYTNLLSNSLKFTRGRDVA
                     RIEVGSYVKDKTTVYYVKDNGIGIPRNEQRKIF"
BASE COUNT          818 a          671 c          716 g          742 t
ORIGIN      
        1 tttcattggt ctcaacgact ttgaattcac tgtccaggac aaggatgagt tcgttagcga
       61 acttcagcat cgatgatatt ggcaccctgt gggagagata gtataccttc gcattcccat
      121 acatcctcat ctccacctgt ccggtgataa ggaggatctc caggtatttt gccaccgaat
      181 ttcggtttat cttcagatta tgagaaatat ctgatatcgt gaggcctttt ggcctggcct
      241 tgagcaattt tttaatccgg gtgatcttct cctggtcgag gatcatggca atcgatcatg
      301 tcatttcggg caaattaaag atttgcaagg cacttgtttt tacaccgtgt gcatttcaat
      361 caaaaccttc aagcactcca agggaggtat acatcatagg ggtggattga acctttttcg
      421 ctttgcgatc tttgaaaggg gctgaagggg atatgacaac gcctgggaag aagcgatcgt
      481 tttcgtttta cctgcttgta ttcttactta tcattatatc agtcatcgtt gcggtaatca
      541 ccatcggcga cctagttttg gtgaacgaaa atttccagga tcatgcagaa ctgctaagaa
      601 atgagaccga gcagaatctg attgaatctg ttgcaaccat tgacaatggc cttaagctct
      661 ttgataatac cctgaacaaa agaatggagc agggctttgt catttttcac cgtggatatc
      721 gtgaagcggg gggtaatcca tcacggatga atctcgccta ccttaaaaag gagatggggg
      781 gcgagatgga tctgtatatc atcaatggat cgggagtggt ggaatacacc acctataccc
      841 ctgaacttgg cctgaacttt aaggaaatga tcccctattt ctatgaatac ctgatggaga
      901 tcaaggcaaa acctggtttc tatcccgatc gggttgttca ggagagtgcc acaggaaatt
      961 tgaaaaaatt tgcctattca cctactgaag atcacgaata tatcctcgag ctcggcttgt
     1021 cagctgaatc gttcaagtca gagaggaata ccctcaagta cactgatatg atcgagtcag
     1081 tccgtcaaag gagcccctat atcagcgatc tcaggatatt cactacggca aaacggcagg
     1141 taggaaacaa gtccttcaag cccgacgcag aaatgaatgc gatccttgac caggttctgg
     1201 ccacacgtca gggtcttgtc atccctgaca atgatccccg tttgacaaca aaatacctct
     1261 tcatcgacct caaggatgag gactacgctg ccgacatgag tcttatcgtc gagattatct
     1321 atgatgatgc actgattttc cagtctcaac aacaattgat cgcttttcat ctccttgttg
     1381 caggatttgg cctcatactc gggattatcg gtgccttcag cctctcccgg taccttaccc
     1441 gaccgatcgc tcagatagca gctgataccg atatcattgc ccatggagac ctcgaccacc
     1501 ggatagcatc aacgaaagga agtgaatttg gcatccttga gaggagcatc aacgcgctgg
     1561 tactatccct caaagggatg atccagcagc tgaagcacag cgagagcaaa ttgcgcgaat
     1621 ctgaagagcg atacaggggt gttgttgaga gccagtcaga gttcatcacg cgattccgtc
     1681 ctgatggaac tatcacattt gcaaatgagg cgtattgccg ttattttggc ctcccggatt
     1741 gtgatatcac tggaaaaaga ttcatccctg ccattcctga tgaagagaga aagagacttg
     1801 acgagggatt ccgtaacctc actccggaga atcccgaact ttcgatagaa cacaggatca
     1861 tcctggcgaa cggagagtcc cggtggcatc agtggaatga ccgcgcaata ttcagcccgg
     1921 aagggaatgt tattgagtac cagtccattg gcagagatat cactgaacaa cggcagattg
     1981 aaaaggatct gatagaaagt gagaggagat tccgtgatct tgcaacactc ctcccccagg
     2041 tgatatttga ggttgatcta aacggcagaa taacctatgt gaacgaatcc gcatatcaga
     2101 tttttggcta taatcccggc gaactggaaa aaggaataac tatttttcag attattgtcc
     2161 ctgaagagca tgaacaggcg cttagaaatc tggaagaatc cattgaaggg aaaaagacgg
     2221 aaggcgcgga atataacatg gtcagaaaag acggatcgaa tctgaacgcc atggtatact
     2281 catcgccgat catgagcgaa ggggccgttg ccggaatccg tggcattctc gttgatatca
     2341 cccagctcaa aaaagtagag gacgatatca ggagactcaa cgaagagctt gagatgcggg
     2401 tgacagaacg gacacgcgat ctggagatgg ctaaccgtga gctcgattca ttctcttatt
     2461 cggtatctca cgatcttcgt gctccactaa gagcaattga tggattttca atgatcctgc
     2521 tccaggaata cggtaagaat ctccccaaag agattgtttc gtatctggag aagataaggg
     2581 aaaataccgg gcgtatgggt gttctcattg atgacctcct gaatttttcc cgcatgagca
     2641 ggcttcccct caaccggttt ttggtggatc cctcctccat tgtgagggaa gcatacgagg
     2701 aattcaggct ggagacgacg gggagacaga ttgatctgat gataaaagag atgcccacat
     2761 gctcggctga tccagcgctc ctgaaccacg tctatacgaa tcttctctcc aattccctca
     2821 aattcaccag ggggcgcgat gttgcccgga tagaggtcgg gtcatacgta aaagacaaga
     2881 ctaccgtcta ctatgtgaag gacaacggga tcggcattcc gcggaacgag caacgcaaga
     2941 tcttcga
//
LOCUS       DAOOCO010000207         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_33252_length_2939_cov_4.871359, whole genome shotgun sequence.
ACCESSION   DAOOCO010000207 DAOOCO010000000
VERSION     DAOOCO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33252_length_2939_cov_4.871359"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK154_09595"
     CDS             complement(<1..318)
                     /locus_tag="PK154_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11351.1"
                     /translation="MNEKQIRITIDPALNETLEHIMKIRNVDNPDQCIGEVLELYSAS
                     LGPVDSIEGDADREREVLLMKIKWLEALLERKERALATLVDLWEECVLDRQARDEELT
                     DIDD"
     gene            complement(420..842)
                     /locus_tag="PK154_09600"
     CDS             complement(420..842)
                     /locus_tag="PK154_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11352.1"
                     /translation="MRRDLLIIALIVVIIAAGAVIAYTYFLGPGSATITSITTDKDLY
                     HSKEVMNITVSASARGDMSNTTLKLVGIKSRHGDYQLDKEIPVTLTPGQNILRYNHTL
                     PTCSSCSGLSAGMYDIDVELVRNGTVVSNMTYSFDLEQ"
     gene            1064..1561
                     /locus_tag="PK154_09605"
     CDS             1064..1561
                     /locus_tag="PK154_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPW11353.1"
                     /translation="MTGDLTLSVVPIEKPEDVNVILGHSHFIKTAEDLYEAVAGAVPG
                     ALFGLAFCEASADRLVRTEGSDEALKELAGKNALRIGAGHSFLLFLRNCYPINVLNTI
                     KNVQEVCTIYCATANPVEVIMVETSLGRGILGVIDGSSPVGVEGPGDVQNRIDLLRKF
                     GYKRG"
     gene            complement(1690..2175)
                     /locus_tag="PK154_09610"
     CDS             complement(1690..2175)
                     /locus_tag="PK154_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPW11354.1"
                     /translation="MTISIIFHSYSGITRGVAEKIQAACGGDLIEVKPKTPYNKITAY
                     TTGSMRARREEADPVEPAEIDLASSDLVVIGTPVWFWKTTPVTNGAIDALRNGEGKKA
                     VLFATCGSKAGETIPIMKKALAGKGVTVIGEHVLTRHDIIKGEKVAEVVDAVKAALNN
                     P"
     gene            complement(2348..>2939)
                     /locus_tag="PK154_09615"
     CDS             complement(2348..>2939)
                     /locus_tag="PK154_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPW11355.1"
                     /translation="VDLAAIDGQGRPIENPTVQIDVESSKRFDIKYYQDGNAVHPPIL
                     HCSPTGSVERVICAILETTSTQKVPMLPVWLSPTQVRVIPVAERHLPFATEVAARIRS
                     AAIRADLDDRDESVGKKARDAAVDWVPFVVVIGDAEMDKGILTVTVRAKSQPNKPHKE
                     TMTEDVLIELMQEETACMPYRPLYTPVQLSKKARYI"
BASE COUNT          624 a          789 c          817 g          709 t
ORIGIN      
        1 gtcatcaata tcagtgagct cctcatccct tgcctgcctg tcaaggacac actcttccca
       61 gagatccacc agggttgcaa gagctctctc ttttcgttcg agcagtgctt cgagccattt
      121 gatcttcatc aggaggacct ctctctcgcg atctgcatcg ccttcgatac tgtccaccgg
      181 tccaagcgag gcagaataga gttcgagtac ctccccgatg cattgatcag gattgtctac
      241 attacgaatt ttcatgatat gctccagtgt ttcattcagt gcggggtcga tggtgatacg
      301 gatctgcttc tcgttcatca gcgatggata aggcgggaac ggtcttcatc gtttccatgc
      361 agggaatccg gcctcacgat gaggatcttc gcggatacca accggcagaa aaaattggat
      421 tattgctcta aatcgaaaga ataggtcatg ttcgatacga cagtgccgtt ccggaccagt
      481 tcaacatcaa tatcgtacat cccggcactc agcccggagc aggatgagca ggtgggaagg
      541 gtatggttgt acctgagaat attctgccct ggggtgagcg tcaccgggat ctccttgtcg
      601 agctgatagt ctccgtgccg gctcttgatc ccaacaagct tgagcgtggt gttgctcata
      661 tcccctctgg cgcttgcaga cacagtgatg ttcatgactt ccttggaatg gtacaggtct
      721 ttgtcggtgg tgatggaggt gatggtagca ctaccgggac ccaaaaaata ggtatatgcg
      781 attaccgcac ccgccgcgat aatcaccacg atgagggcaa tgatgagaag atcacgtcgc
      841 ataaaaaatg gtagtccgtg cttggttatt ataccattgg cgggggatat ggcagatacc
      901 ctttttttca caacgagggt attttgtttt cacactggga gaacggaatc tcaccggcgc
      961 cgatcgttcc tgtcatggtc ccagcttcat ggtttttccc tgctcccgtt cggcaccaac
     1021 gtataaatgt ccctggagcc accatcacgg aatagtgata tccatgactg gcgatcttac
     1081 cctctctgtt gtaccaatag aaaaacctga agatgtgaat gttatcctgg ggcactccca
     1141 ctttatcaag acggcagaag atctctacga ggcggtagcc ggtgcggtcc ccggtgccct
     1201 gttcgggctt gcattctgcg aagcatcggc agaccggctc gtcaggacgg aaggcagcga
     1261 tgaagcgctg aaggaacttg ccgggaagaa cgccctccgt atcggggcag ggcattcgtt
     1321 tctcctgttt ctccggaact gctaccccat caatgtactg aacactatca agaatgttca
     1381 ggaggtctgc accatctatt gtgcgaccgc aaatcctgtc gaggtgatca tggtcgagac
     1441 cagccttgga cgcgggatcc tgggagtcat cgatggatca tccccggttg gggttgaagg
     1501 tccgggggat gtccagaaca ggatcgacct gctccgaaag tttggataca agaggggata
     1561 atgcagagaa accaggagag aagatgagat actgggtgaa ggcttaaaag agatatgagc
     1621 cgggaaaccc acagctctat ccccgtaaga ggggactatc caggagaagg tttcgtaacc
     1681 gagcagaatt tagggattgt tcaacgccgc tttaacggca tccacgacct cggcaacctt
     1741 ctcccctttg atgatatcat gccgggtcag cacatgttcg ccgatcacgg tcaccccctt
     1801 cccggcgagt gccttcttca tgatcgggat ggtctcacct gcctttgagc cgcaggttgc
     1861 aaagagcacg gccttctttc cttcaccatt ccggagggca tcgatagcac cgttggtgac
     1921 cggggtagtc ttccagaacc agaccggcgt tcctatcacg accaggtccg atgatgcgag
     1981 atcgatctcg gcgggttcga ctggatcagc ttcctcgcgc cgggccctca tgctcccggt
     2041 ggtatatgcc gtgatcttgt tgtaaggggt ctttggcttc acttcaatga ggtctccccc
     2101 gcaggctgcc tgtatcttct cggcaactcc gcgggttatc cccgaatatg agtgaaaaat
     2161 gatggagatc gtcatgaggg gtactcttcc tttttatcta atattcctgc cgaataccag
     2221 tgaggaagag gaagagggac ctgggacgtc tgcgatgttc cctggaacgt cagtctccat
     2281 gagtgcccct ttcggtagca tgcaactgct ggttcctgtc caatcctgaa aatatggatg
     2341 cgggttatca gatataccgg gctttcttgg agagctggac cggcgtgtag agcggccggt
     2401 agggcatgca tgcggtctct tcctgcatga gctcgatcag cacatcctcg gtcatcgtct
     2461 ccttgtgcgg cttgttcggc tgggactttg ccctcaccgt aacggtcagg atccccttgt
     2521 ccatctcggc gtcaccgatg acgaccacga acggtaccca gtcaactgcc gcgtcccttg
     2581 ctttctttcc gacactctcg tcccggtcat cgaggtcggc acggatcgcg gcactacgga
     2641 tccgggcggc aacctcggtg gcaaacggga ggtgacgctc tgctaccggg atgaccctga
     2701 cctgggtggg tgagagccag accgggagca tcgggacctt ctgcgtgctc gtggtctcga
     2761 ggatggcaca gatgacccgc tccacgcttc cggtcggtga acaatgaagg atggggggat
     2821 ggactgcgtt cccgtcctgg taatacttga tgtcgaaccg tttcgaggac tcgacatcga
     2881 tctggacggt cgggttctcg atcggccgtc cctgcccatc gatggcagcc aggtcgacc
//
LOCUS       DAOOCO010000208         2910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_33748_length_2910_cov_5.546760, whole genome shotgun sequence.
ACCESSION   DAOOCO010000208 DAOOCO010000000
VERSION     DAOOCO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33748_length_2910_cov_5.546760"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK154_09620"
     CDS             complement(<1..242)
                     /locus_tag="PK154_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11356.1"
                     /translation="MNKLIGRMEDPRETLDYSYEKQLDLLQNVKRGVAEVATSKKRLQ
                     LQRAKLLESSEKLEAQAREAVAVNREDLARLALERK"
     gene            453..1028
                     /locus_tag="PK154_09625"
     CDS             453..1028
                     /locus_tag="PK154_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPW11357.1"
                     /translation="MDIVADLMKQVATGDNLSQITKAVGGDEKGVSSALGMALPMILG
                     SMSNTASKQGGADMITGMLGQMGGQNPMDNLGGFLGGSAAAGGSSMVSSLLGSQMGTI
                     QNAIAKKTGLPPEIVGKVLAIAAPMVIGYIGKMFAGKKVEPQALTSMLGEQSKLAMKA
                     SPEVASMAQELMGAQKEATGISGFFKKLFGK"
     gene            1234..1806
                     /locus_tag="PK154_09630"
     CDS             1234..1806
                     /locus_tag="PK154_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPW11358.1"
                     /translation="MTDIIVYEERICTVKWGTFIILGLLALIFGILIFIYPEVTAAVL
                     VMLFGVLIIVLALLALVAALMSPGGSGRPSLLLFAAILGFIVGAAALVAPQIFGAILT
                     IIIAVVLFVIGIMNVTLALSEKDYPHRWLVFLLGILSIIFAILIMIYPLIGSIIIFGY
                     LIGLYFVLYGILSLIAGFALRSVQKEYCKV"
     gene            complement(1947..>2910)
                     /gene="cls"
                     /locus_tag="PK154_09635"
     CDS             complement(1947..>2910)
                     /gene="cls"
                     /locus_tag="PK154_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPW11359.1"
                     /translation="DPLSRDLISALTVKAQEGVEVRLLADSLGMKVKKKELEKFLQAG
                     GKYAAFFPRLYTLNYRNHRKIAVIDGTSGFIGGYNIGEEYLGKGPLGYWRDTAIRIDG
                     TGVNGLQLRFFMDWNYASDEKISFDPVYFPEKTGHGTCPLQIVSSGPDARWNQIKDGY
                     VKLIQSAMESVYIQTPYFIPDESISDALRIAAHSGVDVRIMIPCKPDHPFVYWSTLSF
                     IGDLLDSGVRAYTYDNGFIHAKTIVVDGIAASVGSANFDVRSFRLNFEANAFFYDPEY
                     GGQMMKAFLDDLPKCTEITLEGYKNRSRWIKVKESVSRLFSPLG"
BASE COUNT          684 a          752 c          695 g          779 t
ORIGIN      
        1 tgtttccgtt cgagggcgag gcgtgcgaga tcttcccggt tcactgccac tgcttcccgt
       61 gcctgggcct cgagcttttc actgctctcc aacagctttg ccctctgcaa ctggagccgt
      121 ttcttggagg tggctacctc cgcgactccc cgtttcacat tctgcagcag atcgagctgc
      181 ttttcatacg aataatcaag cgtctcccgg ggatcttcca tccgtccgat caacttgttc
      241 atcttcgcct tgattacggt acccatgcgg tttaaaagac ccattgtatc atctccctgt
      301 cttcattgct gcgaagacgg ttacttgtaa tgagcaacga gatgggagaa atagcttttg
      361 accgggaaac ccccaatcaa aaaggaatga ttaccttact cccaagttcc atacatagct
      421 ttaatcccca tcccctgcca tccttatgca ttatggacat tgttgcagat ttaatgaaac
      481 aggtggcgac gggagacaac ctctctcaga tcaccaaagc agttggcggc gatgaaaaag
      541 gagtctcatc agcactaggc atggcgcttc cgatgatcct cggttccatg tcgaacactg
      601 catcgaaaca gggtggagcg gatatgatca ccgggatgct cggacagatg ggtggccaga
      661 acccgatgga caacctggga ggttttctcg gaggttccgc tgcagcaggt ggatcgagca
      721 tggtcagcag ccttctcggg agccaaatgg ggaccatcca gaacgctatc gccaagaaga
      781 ccgggcttcc gccggagatt gtcggcaagg tactggccat tgctgccccg atggtcattg
      841 gctacatcgg caagatgttt gccggaaaga aagttgagcc ccaggcactt accagcatgc
      901 tcggtgaaca gtcgaagctc gccatgaagg cttcaccaga agttgcaagt atggcccagg
      961 agctgatggg tgcacaaaaa gaagcaaccg gtatctcggg ctttttcaaa aagttgtttg
     1021 ggaaataaac cagttcttgc cttctccttt ttttattccg gccttgtcat ggctgaaagg
     1081 atatctgtca tggatgagaa ccgggattta actaacatac tgccctaaaa agatctggta
     1141 tttctatttc atccttccca gattgaaaaa tcgacaattg aaacagaaaa gatatttata
     1201 gcaagaattt tcaggagcta ctgaggtcat cagatgactg acattattgt gtatgaagaa
     1261 cggatctgta ccgtaaagtg gggtacattc attattctcg gtttacttgc attgatcttc
     1321 ggtatcctga tctttatcta tccggaagtg actgctgcag tccttgtcat gctctttggc
     1381 gtattgatca ttgtgcttgc gcttctggct cttgtggcag cattgatgag tccgggggga
     1441 tcagggcgtc catcgttatt gctgtttgcg gcgattcttg gattcatcgt tggagccgca
     1501 gcccttgtag caccccagat ctttggagcg atcctgacaa tcatcatcgc cgttgtcctg
     1561 tttgtaatcg gtataatgaa tgtgaccctt gctctgtcag agaaggatta cccccatcgc
     1621 tggctggtgt tcctccttgg gatcctctcg atcattttcg caatcctgat catgatctac
     1681 ccgctcatcg gttcgatcat tatcttcggg taccttatcg gcctgtattt tgttctatac
     1741 ggaatactct ccctcattgc cggatttgct ctgaggagcg tgcagaaaga gtattgcaag
     1801 gtataactcc aatcaccttt ttctcctgac aaaaccgcgt tttccacaaa ggagttgctg
     1861 aagagcgatt ccatatgaat cctggtgacc tgaattcccc catcataaaa acagaatttt
     1921 tcttccggaa ggaaatgtgg agatgatcac ccaagcggag agaagagtct tgagaccgat
     1981 tctttcacct ttatccatcg tgaccggttc ttatacccct caagcgttat ttcagtacat
     2041 ttcgggagat cgtccaggaa tgctttcatc atctgacctc catattccgg atcataaaag
     2101 aaggcatttg cctcgaagtt cagcctgaag ctccggacat cgaaattggc acttcccacc
     2161 gaggctgcga taccatcgac cacgatcgtc tttgcgtgaa tgaatccatt atcgtacgta
     2221 taggctctca ccccggaatc gaggaggtct ccgataaatg agagggtcga ccaatagaca
     2281 aaggggtgat cgggtttgca gggtatcatg attctgacat ccactccgga atgtgctgct
     2341 atccggagag cgtccgatat gctctcatcc gggataaaat agggggtctg gatatatacc
     2401 gattccatgg cgctctggat cagcttcaca tatccgtcct tgatctggtt ccagcgtgca
     2461 tcggggccgc tcgagacgat ctggagcgga cacgtcccat gccccgtctt ttcagggaaa
     2521 taaacgggat cgaaggagat cttttcgtcc gatgcatagt tccagtccat gaagaaccgg
     2581 agctgcagac cgttgactcc cgtcccatcg atccggattg ctgtatcacg ccagtatcca
     2641 agcggccctt tcccaagata ttcttcaccg atattgtatc ccccgataaa tcctgaagtg
     2701 ccatcgatca ccgctatctt ccggtgattc cggtaattca gggtgtataa acgggggaaa
     2761 aatgccgcat attttccacc tgcctggaga aacttctcca attctttctt cttcactttc
     2821 atccccagtg aatcggcaag cagcctgact tccaccccct cctgtgcctt cacagtgagc
     2881 gccgagatca gatcccgaga gagcgggtca
//
LOCUS       DAOOCO010000209         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_34720_length_2856_cov_4.963941, whole genome shotgun sequence.
ACCESSION   DAOOCO010000209 DAOOCO010000000
VERSION     DAOOCO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34720_length_2856_cov_4.963941"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..847)
                     /gene="pstB"
                     /locus_tag="PK154_09640"
     CDS             complement(8..847)
                     /gene="pstB"
                     /locus_tag="PK154_09640"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPW11360.1"
                     /translation="MVIPLHLAFIENSGIKTAKPEIPSAYDASNPVIEARNLNLWFGD
                     AHILKEVSMRIRRNKVTALIGPSGCGKSTLLRCFNRLNDLIDHTKITGEVTFDGENIY
                     AAGNDVVLLRKRIGMVFQKPNPFPKSVYENIAYGPRVHGISDKKELDRIVEKSLKDAA
                     LWEEVRDRLNNSALGLSGGQQQRLCIARTLAVDPEVILMDEPCSALDPIATAKIENLI
                     EKLKQQYTVIIVTHNMQQAARVSDDTGFMYLGKLVECGTTTQIFEHPKEDLTENYITG
                     RFG"
     gene            complement(841..2754)
                     /gene="pstA"
                     /locus_tag="PK154_09645"
     CDS             complement(841..2754)
                     /gene="pstA"
                     /locus_tag="PK154_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPW11361.1"
                     /translation="MTTPAIRRIQEQAIGSLWFTCAIFAVLTVCFILGYLFYNGYPAF
                     VQIGLQDFLFGLDWNPTGSPPSYGILSLIVGTILVMIGAMVLAVPLGIASAIFISELA
                     SPRLRNIIKPAVELLAGIPSVVYGFFGLVVLTNWIRVSFDVPTGETWLAGSILLGIMA
                     LPTIVSVSEDAINSVPREFKEGSLAIGATGWQTISKVIIPAALSGITAAIILGIGRAI
                     GETMAVLMVTGNAAVVPEPIWNVLSPIRTLTATLGIETGEVPIGSLHYNALFAIAVVL
                     LIITLIINLSATFILDRIRHAQTGSADTKRLFSQDTSVQIRRFLQYGFLGMVLLLVVV
                     AAGILPCLVLLVVLIAISILWKRVSPKITQKIAYSCMFAAVGIVLAILAIILLDIIMK
                     GLPVISWEFIFGYPSNLGRAGGIFPAIVGTIYLVIGAIMIALPIGIGATIYLSEYTRE
                     GLVTKTIRTGVDLLNGTPSIVFGLFGFAFLVIFVGLGVSLLTGWIVLALMILPTIIRT
                     SEEALKSIPNAVREGSLALGASKWQTIRKVVLPAAVPGIITGTILSIGRAAGETAPIL
                     FTAVVFSTRFLPSSIFEPVMALPYYLYILSTSIPGAKQYAYGTALVLLVIVTLFYGAA
                     VLLRTRFQKSIRW"
BASE COUNT          717 a          707 c          775 g          657 t
ORIGIN      
        1 gttcctccta gccgaatcgt ccggtgatat aattctcggt gagatcttct ttggggtgtt
       61 caaatatctg tgtggtcgtt ccacattcga ccagtttgcc cagatacata aatccggtat
      121 catcgctgac ccgtgccgcc tgttgcatat tatgggtgac aatgatcacc gtgtactgct
      181 gcttcagctt ctctatcagg ttctcgatct ttgcggttgc tatagggtca agtgccgagc
      241 atggctcgtc catcaggata acctcgggat caaccgccaa ggttcgggcg atgcagagcc
      301 gctgctgctg tccgccggaa agaccaaggg cagaattgtt cagccggtcc cttacttcct
      361 cccaaagtgc tgcatctttg aggctcttct caacgatccg gtcgagttct ttcttgtcgc
      421 ttattccatg gacccgtggt ccgtatgcaa tattctcata cacacttttg gggaagggat
      481 tgggtttctg gaacaccatg ccgatccttt tcctcaggag gacgacatcg ttcccggctg
      541 cataaatgtt ttccccgtca aaggttactt cacctgttat tttcgtgtga tcaatgaggt
      601 cattcaaacg attgaaacac cggaggagtg tggatttccc gcatcctgac ggccctatca
      661 gggcagtcac cttgttccgc cggattctca tgctgacctc tttcaggatg tgggcatcac
      721 caaaccagag attgagatta cgcgcttcga taactggatt ggaggcatca tatgctgaag
      781 ggatttctgg ttttgctgtt ttgatgcctg aattttcaat gaatgcgaga tgcaagggga
      841 tcaccatctg atggatttct ggaagcgggt acggagtagg actgcagccc cgtagaacag
      901 ggttacaatg accagcaata caagggctgt tccgtatgca tattgctttg cgccggggat
      961 gctagtcgag aggatgtaaa ggtaataggg gagggccatc accggttcga atatcgagct
     1021 ggggagaaaa cgggtggaga ataccaccgc agtgaacagg atgggggcag tctcacccgc
     1081 agcacgacca atcgagagga tggttcctgt gatgatacca gggacggctg cagggaggac
     1141 cactttccgg atggtctgcc atttcgaggc tccgagcgcc aggctcccct cgcgcactgc
     1201 attcggaata ctctttaaag cctcctcgct tgtgcgtatg atagtcggga ggatcatgag
     1261 ggcgagcaca atccacccag tgaggagcga cacccctaac cctacaaaaa tgacgagaaa
     1321 tgcgaatcca aagagaccaa atacgatcga aggagtgccg tttaggagat ctacccccgt
     1381 ccggatagtt ttagttacca ggccttccct ggtatactcg gagagataga tcgtcgcccc
     1441 gatgcctatc ggcagagcga tcatgattgc cccgattacc aggtaaatgg tcccgacaat
     1501 cgccgggaag attccgccag cccttccgag atttgatgga tagccgaaaa tgaattccca
     1561 ggagatcact ggaagtcctt tcataatgat atcgagaaga atgatggcaa gtatggctaa
     1621 gacgatacct actgccgcga acatgcatga gtaggctatc ttctgggtaa tcttggggga
     1681 taccctcttc cataggatgg atattgcgat aagcacaaca agtagcacca ggcaggggag
     1741 aattccggca gcaaccacaa ccagcaacag aaccatacca aggaaaccat actgcaggaa
     1801 acggcggatt tgaacacttg tatcttgaga gaacagtcgt tttgtatcgg cacttccggt
     1861 ctgagcatgg cgaatacgat cgagaatgaa ggtcgctgat aaattgatga taagggtgat
     1921 gatcagcaaa acaacggcaa tcgcgaagag tgcattgtag tggagactcc ctatcgggac
     1981 ttcgccggtc tcgataccaa gggttgcggt cagggtgcgg atgggagata atacgttcca
     2041 gataggttca ggcacgactg ctgcattccc ggtcaccatc agaaccgcca tggtctcacc
     2101 gatagccctg ccgatcccga ggatgatcgc cgcggtgata cctgaaagag ccgcggggat
     2161 gatcaccttg ctgatcgtct gccagccagt ggcacctata gcgagtgagc cttctttaaa
     2221 ttcacggggc accgaattga tcgcatcctc agagacgctg acaatcgtgg gaagtgccat
     2281 aattccgaga aggattgatc ccgcaagcca ggtctctccc gtcgggacat cgaaggagac
     2341 acggatccag ttggtaagta ccacaagccc gaagaaacca tataccacgg aaggaatacc
     2401 tgcgagcagt tcgacggcgg gtttgataat gttccggaga cggggactag cgagttcgga
     2461 tatgaagatg gcgcttgcaa tcccgagcgg gaccgccaga accatggcgc cgatcatcac
     2521 aaggatagtt cccacaatca gtgagagaat tccataactt ggtggactgc cggtggggtt
     2581 ccagtccagg ccgaatagga agtcctggag gccaatctgg acaaatgccg gatatccatt
     2641 gtagaagagg tagccaagaa tgaaacatac cgtcagcacc gcgaatatgg cacaggtaaa
     2701 ccagagcgac ccgattgcct gctcctggat tctccggatt gcaggagtag tcattcggtt
     2761 cctgatatct ggcaaggcca tatgcatcac accgatgact gtatatagga cgttcggttt
     2821 aaaaaccgat aggagcctgc acttttttct tcagag
//
LOCUS       DAOOCO010000210         2846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_34915_length_2846_cov_4.405231, whole genome shotgun sequence.
ACCESSION   DAOOCO010000210 DAOOCO010000000
VERSION     DAOOCO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34915_length_2846_cov_4.405231"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PK154_09650"
     CDS             <1..1298
                     /locus_tag="PK154_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW11362.1"
                     /translation="FLISKVPRKPYIPSLSAALVAVALTWLAAAVIGAVPFVLALEVP
                     WTDGIFESMSGWTDTGITVMKSIDTMPKTLIFWRSFTQWLGGIGVIAFGIAMLDRSGL
                     SQSQLYRSEGRSEDLMPNVVSTGRRIWLIYVTLTLLSTGLILLSDIHLWDAVNLAMVA
                     ISTGGFAPHDAGMSYYNSASLEFLLIPVMIAGALPFKVYFLMYRGKAREIFKNSVVRL
                     IVVLSLAGSAIVVLNLYILHTISLFDAVRMGTFCTVSGLTTTGFQNSSLLWLPLPLLV
                     IMLLMLIGGASGSTAGGIKANRIILGYEGLVWWFKRLFVRGSVMVPFKHEGRNIPAKL
                     SELELSKNMLIISLYLLTVIVAVILALHLARTQFEVYQVTFEVISAVANNGMSFGYLT
                     ATSPVTVKWLFILVMWIGRLEILPVLILIGGLVRGFDLR"
     gene            complement(1363..1797)
                     /locus_tag="PK154_09655"
     CDS             complement(1363..1797)
                     /locus_tag="PK154_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW11363.1"
                     /translation="MDFSECIGFANANPVTYIATIDGDQPRVRAFAMWFADETGFYYH
                     TGTPKSIYSQLSKNSKTELCFYAPSPDGAGRMMRVAGSVEFLNDTALEERLYRDRPWV
                     KDLVKAGPRDAKVAIFRVAHGEAYLWTMEYNMREREAPRVRF"
     gene            complement(1928..2716)
                     /locus_tag="PK154_09660"
     CDS             complement(1928..2716)
                     /locus_tag="PK154_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPW11364.1"
                     /translation="MEKNPLIIQHLSAGDFRKEPFEKVILPLGSLESHGDHLPFGTDS
                     LTAYLLSLEVARRVPKTAVLPPLPYGMSEAYSEFSFTVSLRPETETAIINDILESLYQ
                     EGIRKVFILNGHDGNIASIEMAARTVKAAHPEMKILSLDAWWNTLKELLPPGFFEVWE
                     GLGHGGEGELSMGLALFPDLCEPEQARGIVPRLPPYVDVKWMFSELTDCGATGDPTKA
                     TGEKGFRMKEVLVNAIVGVLTSLDESGWDYRSPELKGNQNRERR"
BASE COUNT          615 a          763 c          744 g          724 t
ORIGIN      
        1 ggtttctcat ctccaaagtg ccgagaaagc cttatattcc ctccctctct gctgcacttg
       61 tggcggttgc cctgacatgg cttgccgcgg ctgttatcgg ggctgttccc tttgtgctcg
      121 ccctggaggt gccctggaca gacggtatct tcgaatccat gtcaggctgg accgatacgg
      181 ggatcaccgt catgaaatcg atagacacca tgccgaaaac tcttatcttc tggcgatcat
      241 tcacgcaatg gttgggaggg attggggtga tcgcattcgg gattgccatg cttgaccggt
      301 ctggtctctc gcagtcccag ctctaccgtt cggaggggag atccgaagac ctgatgccga
      361 acgttgtatc caccgggaga aggatatggc ttatttatgt caccctgacc ctcctttcaa
      421 cagggctcat cctcctgtca gacattcacc tctgggatgc cgtgaacctt gcgatggttg
      481 cgatctctac cgggggtttt gcccctcatg atgccggaat gtcctattat aacagtgctt
      541 cgctcgaatt tctcctgatc ccggtgatga ttgcaggagc gcttcctttc aaggtgtatt
      601 tcctgatgta ccgaggaaaa gcaagagaaa tattcaaaaa ttccgtcgtc cggctgatag
      661 tagtcctctc tcttgccggt tcagcaatcg ttgtcctcaa tctgtacatt ctccacacta
      721 tctccctttt tgacgcagtc aggatgggga cgttctgcac agtatccggg ttaaccacca
      781 ccggatttca gaactcatcc ctcctctggc tcccactccc tctgctggtt atcatgctcc
      841 tgatgctgat aggaggtgcc tcagggagta cggcaggggg cattaaggcc aaccgtatta
      901 ttctcggata tgaaggcctg gtctggtggt tcaaacgcct gtttgtcagg ggaagcgtga
      961 tggttccctt caaacacgaa gggagaaata ttcccgcaaa actttctgaa ctcgaactat
     1021 cgaagaacat gctgatcatc agcctgtacc ttctgaccgt gatcgtggca gtcatcctcg
     1081 ctctccatct tgctcgaacc cagtttgagg tttaccaggt taccttcgag gtcatctccg
     1141 ctgtcgccaa caatggtatg agttttggat acctgaccgc tacaagcccg gtaacggtca
     1201 agtggctctt tatcctggtg atgtggatcg gcaggctgga gattctcccg gtgctcatcc
     1261 tgatcggggg gcttgtccgg ggtttcgact tgcgctgaaa aagcagggca ttttatctac
     1321 cggtctttgc gatgagacag ggcacaatac agactggcaa aatcagaacc tcacacgggg
     1381 tgcttcccgt tcccgcatat tatactccat cgtccagaga tatgcctccc cgtgggctac
     1441 cctgaagatg gcaactttgg catccctcgg ccctgccttc acaaggtctt tcacccacgg
     1501 ccgatcgcgg tagagccgct cctcaagggc tgtatcgttg aggaactcga cactgcccgc
     1561 cacccgcatc attctcccgg ccccgtccgg acttggtgca tagaaacaga gctcagtctt
     1621 tgaattcttt gagagctggg aatagatact cttcggagtg ccggtgtgat agtaaaaacc
     1681 ggtctcgtcg gcaaaccaca tcgcaaatgc cctgacacga ggctgatctc cgtcaatagt
     1741 ggcaatgtat gtaaccggat ttgcgttggc aaacccaata cattcggaaa agtccatgat
     1801 gatatcccgc tactcaatgg aagaaaaaag atgaaaaaga ttgggtcacc ctgatgccgt
     1861 gcatcacggt tggtgaccct gccatccctt caagtaaaaa ttcccagcct gtattccaga
     1921 aaccaggtta tctcctctcg cgattctggt tacccttcag ttccggcgac cggtagtccc
     1981 accctgattc gtccaggctg gtaagtaccc caacgatggc attgacaaga acctctttca
     2041 tccggaaacc tttctctccg gtggctttcg tgggatctcc ggttgcacca caatcggtca
     2101 gctctgaaaa catccatttt acatcgacat aggggggaag ccgtggaact atcccccgtg
     2161 cctgctctgg ttcgcagaga tcgggaaaga gggcaagacc catcgatagt tcaccttccc
     2221 caccatgccc gaggccttcc cacacttcga aaaaaccagg ggggagcagc tccttcaggg
     2281 tattccacca ggcatcgaga gagaggatct tcatctcagg atgtgctgct ttaaccgtgc
     2341 gggctgccat ctcgattgag gcgatattgc catcgtggcc attcaggatg aagacctttc
     2401 tgatcccttc ctgatacagg gattcgagaa tatcattgat gattgcggtc tcggtctcgg
     2461 gacggaggga gacggtgaag gagaactcgc tgtacgcctc gctcatccca tacgggagag
     2521 gggggaggac ggctgttttg ggaacccgtc gggcgacttc gagggaaagc aggtaagccg
     2581 tgagtgaatc ggtgccaaac gggagatggt ccccgtggct ttcgagcgag ccgaggggca
     2641 ggatcacctt ctcgaaaggt tctttccgga aatctccggc agagagatgc tggattataa
     2701 gaggattttt ctccatggtg catgtccctg tctgtgcttg gatcatggtc tctcaaataa
     2761 gctttggact gctcgttcct cattggtgga tcttataggg agggtgaagg ggaagtatga
     2821 gatacggagt cctgtcttat tttttt
//
LOCUS       DAOOCO010000211         2843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_34968_length_2843_cov_4.758967, whole genome shotgun sequence.
ACCESSION   DAOOCO010000211 DAOOCO010000000
VERSION     DAOOCO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34968_length_2843_cov_4.758967"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK154_09665"
     CDS             <1..298
                     /locus_tag="PK154_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11365.1"
                     /translation="DVARECEASSLSDFLLLPDLLASFGDLDYDVVEMVLADQGSWDR
                     YEAAKWLTMRRWLDANPDDDFAKEVRARLTSEPQRYAAYTREYLGWGVFALMAR"
     gene            complement(560..1129)
                     /locus_tag="PK154_09670"
     CDS             complement(560..1129)
                     /locus_tag="PK154_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11366.1"
                     /translation="MNRISAFKSIALFILLVFVLVCAGCTQPYEVTNQTASPTTTPAP
                     AQTRSTDYGTQKTELAERAASFASQIDNRTFIAALTEGPNSTAFATVLAQVKAFQATD
                     SRIAFVYILEQQNGVARFVVVSDYGMPGSATFMMVYPGAPEELKTPITAPMGVGPYTD
                     PWGTFLSGYAPVTMSTNEATYIMAVDMRG"
     gene            complement(1409..1837)
                     /locus_tag="PK154_09675"
     CDS             complement(1409..1837)
                     /locus_tag="PK154_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11367.1"
                     /translation="MDTTFCHYYRGTGFPPTNRFCRECPASAIACDRLWQMVVDLSNA
                     KNGSPVSLPNTRAVLTPNPKNGNIVHLQINCRWSLGKEDFLHYIATGQAQLGRKTQRL
                     DPAVSPSMTRQVPYVQSIVQALGGPHLPEIVAVKKVQKGE"
     gene            complement(2090..2377)
                     /locus_tag="PK154_09680"
     CDS             complement(2090..2377)
                     /locus_tag="PK154_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPW11368.1"
                     /translation="MRKVTINAIVDIIMVIAFIPTLLSGVVLYWFLPEGGRFSGQAVF
                     MGLSRQIWITMHDRWGIIFSIIVILHLLLHWKFLRVLPACFRKSKKEEVVE"
BASE COUNT          701 a          688 c          768 g          686 t
ORIGIN      
        1 agacgttgcc agggaatgcg aagccagttc tctctccgac tttctcctgc tgccagacct
       61 tctcgcgtcg ttcggcgacc tcgactacga tgttgtggag atggttctgg ccgaccaggg
      121 gagctgggac cggtatgaag cggccaagtg gctcaccatg cgccgatggc tcgatgcgaa
      181 tcccgatgac gactttgcaa aagaggttcg tgccagacta acttcggaac cacaacgcta
      241 cgctgcgtac acgcgtgaat accttggatg gggggtgttc gcgctgatgg cacggtgagg
      301 cagcggagat attcggcgga ttgtcaacgg ctgggctacc ctccatcact cgtcgaaggg
      361 tgaattcatg attattacag aacacccctt caacacttcg aaacgttgag gctttagggg
      421 gggagagggc tctcatcgag taacgcccct taatggattc ccgatggtct ttttttccga
      481 aacagaggag agggaggatg gtatttttta acgagcagtg atgtgatacc cctttcaaag
      541 gggagggggt gagggaggat taccccctca tatctactgc catgatatag gtggcttcgt
      601 tcgtactcat cgttacggga gcataccctg aaaggaatgt cccccagggg tcagtgtagg
      661 gacccactcc catgggtgcg gtaatcgggg ttttcagctc ctccggtgcg ccgggatata
      721 ccatcatgaa tgtcgcactg cccggcatgc cataatcgga aacgacgacg aaccgggcga
      781 cgccattctg ctgttcaaga atatacacaa aggcaatcct gctgtcggtt gcctggaatg
      841 ccttgacctg tgcgagtact gtcgcaaacg ccgtagagtt tggtccctcc gtcagtgcag
      901 caataaacgt cctgttgtca atctgactgg caaatgaggc agcacgctcg gccagttcag
      961 ttttttgtgt cccgtagtct gtcgatcttg tttgcgcggg agcgggggtt gttgtgggcg
     1021 atgcggtctg gtttgtcact tcgtatggct gggtacagcc ggcacacaca agaacgaaca
     1081 caagcaggat gaaaagagct atggatttga atgctgaaat gcggttcatg ctgaaagatc
     1141 aatgaacggg tataaatgtg tgatgatcgt caggaaccat ccccctctca tgatcgttcc
     1201 tgacctgcgg ttcggatagg aataggaata ttcccaccgg gtttcggttc ccctttttct
     1261 tattccagga atccctgcat cagttggggt gtaccctgat gaaaatcaag tgactccggt
     1321 agggagcacg aagtatggta ctataaaaaa gaatcaaagt tgggccgact attgggttct
     1381 cttaaggaaa gacttcactt atacggggtt actctccttt ctgcaccttt ttcaccgcca
     1441 caatttcagg gaggtgaggt ccgccaagag cctggacgat cgattgcaca taggggacct
     1501 gccgggtcat actcggtgaa acagcaggat ccagtcgctg ggttttccgt ccgagctggg
     1561 cctgcccggt cgcaatataa tggaggaagt cctcctttcc aagactccat cgacagttta
     1621 tctgtaaatg aacaatgttc ccgttcttgg gattgggagt gagaacggct cttgtattcg
     1681 gcaaggaaac aggagagcca ttctttgcat tcgacagatc taccaccatc tgccagagcc
     1741 tgtcacaggc gattgccgaa gcaggacatt cgcgacagaa cctgttggtg ggaggaaaac
     1801 cggttccccg gtaatagtga caaaatgtcg tatccatgga aagaactcac gttattttcg
     1861 gagggaatag gttaaaaatc atgggtatct cgatggggag aatgatcacc gggatgagag
     1921 gaccgaatct ttgttttccc atgtgccgtt tcacatcacc accgttccgc ttacgccatt
     1981 ctcgccaacc atcaattccc tcactccggc agcgttcaga agcacgggaa cggtcgatta
     2041 ctgcattatg ctgccccgat gaaacctttc ggtgaaaaaa ggaaacatgc tattccacga
     2101 cctcttcctt tttacttttc cggaaacatg cgggaaggac ccggagaaat ttccaatgaa
     2161 gcaggagatg caggatgacg atgatggaaa agataattcc ccaccgatcg tgcatagtga
     2221 tccatatctg ccgggagaga cccataaaga ccgcctgacc gctgaatctc cccccttccg
     2281 gaaggaacca gtagagcacc actcctgaga gaagggtggg gatgaatgcg atgaccatga
     2341 tgatgtccac gatggcattg atcgttactt ttctcataga atcacgtatg atggtgatag
     2401 gggggatccg atattaatga gatcgaaaat atccttcccg gagactatct ccggttacca
     2461 ccatcgtgtc gggatatcct tctccaccag gggaggtagt gtatcctgtt gtgaaggggg
     2521 agagggggat ttgaatatgt ttttcgagag tgcgtcattc tctccaccct cttcagtcca
     2581 tgcctccggc catgcatccc gggaacgatc acatgccaga tctgtgccga tttcgaggac
     2641 gtcccgcttt tacccgaaac aacaatacag ggatcttcga atcaggcagc cttgaattcg
     2701 ccgatcagtt tttgttcagt aagagaccaa aaaaaagaac acaactataa tatagtaaaa
     2761 ccatatgata gaaccattgt gagtaagtcc ttcccgaaac ggatgaggct accttgaggt
     2821 aggtgggaca aagtagggaa ggg
//
LOCUS       DAOOCO010000212         2839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_35059_length_2839_cov_4.223060, whole genome shotgun sequence.
ACCESSION   DAOOCO010000212 DAOOCO010000000
VERSION     DAOOCO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35059_length_2839_cov_4.223060"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK154_09685"
     CDS             complement(<1..304)
                     /locus_tag="PK154_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPW11369.1"
                     /translation="MTSNRPLTMNDHALKECMQAAVKEARRGLAEGGIPIGSVLVIDG
                     KIVGRGHNRRIQKGSAVLHAEMDCLERAGRLGYRDYSRSVLFSTLSPCDMCSGAVLL"
     gene            369..1625
                     /locus_tag="PK154_09690"
     CDS             369..1625
                     /locus_tag="PK154_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11370.1"
                     /translation="MKKILLILLLILLIASSGCIGRHRENGSGPPTITPTTGPEGQPI
                     VTSSRPAATSTPCKELNPLIILVEFPDKKHVAERNFFVNRYNTQVNTYLQSVSYGAVC
                     LNGEVTPTWYTLPLETTSYRISSRNLEVNRTRVDNLIRDALAAADADYNFSDYDFVVI
                     QLGVLTPDYGMVGLCGYPGMLGWTKTSVLTAPSGEVVQRGVAIFTAQAHVGTNFHDIA
                     HILGGVKDGNRMVPCLYDHDLQAKPGPDLEVFRNSMINMGYWDPMSCHFYRNDTSPPG
                     ICSWTRIRLGWLDEEKILTVDPDNQTEVMLGPLEDTSSEVLVIRIPLSSSTYYLVENR
                     APIGVDRVLPDHGILIMFADDRIAECHHGEAPVKLIDANPAIPYLEGAAFDIGEKDEF
                     VDQANNLRIKLLEKSGSEYRILIEPL"
     gene            1991..>2839
                     /locus_tag="PK154_09695"
     CDS             1991..>2839
                     /locus_tag="PK154_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW11371.1"
                     /translation="MGKSHIVQYLFTAGFILLVLLLLMHPSAPDLFPSRDSQPSVSLL
                     YASSGSMAQLLNTGQIDAFLIWEPIVANAELSGIGKRIAVPSDLPPPGKWDNAAINIL
                     VLRQDTIRAHPDLAALLSALTTAAIDRTNEDPTLAENITAHWVYGKGPILTPQGTLDP
                     LTVEQRSFENMVFTPDAAPPEASIVEYTIDSMTGGTGSYDPMMWVDSTVLARAAYFLN
                     GTAVPTIDPALPTLNIGYIPSSDNYAPVYVMVKDSEYFCDRYGFCLVPDDPSLSRPVT
                     CTLLVNG"
BASE COUNT          664 a          831 c          679 g          665 t
ORIGIN      
        1 ataagaggac agcgccactg cacatgtcgc agggggagag agtagagaag agtaccgacc
       61 gtgaatagtc ccggtatccc agccggccag cacgctcaag gcagtccatc tcggcatgga
      121 gcactgcact ccctttctgg atccggcggt tatgccccct cccgacaatt tttccatcga
      181 tcaccagtac cgagccgatg ggaatccccc cttcggccaa tccccgccgt gcttccttca
      241 ccgcagcctg catgcattct tttaacgcat ggtcattcat cgtgagaggt cgattgcttg
      301 tcattgacga tatccgcccc ctgttctgga ataccttttt ctcattatgt actgatccct
      361 gatgcgctat gaaaaaaatt cttctgatct tgctgcttat cctcctgata gcgtcctccg
      421 ggtgtatagg aaggcaccgt gaaaacgggt ccgggccccc gacaataaca ccgacaaccg
      481 gcccggaggg ccaaccgata gtcacttctt ccagaccggc tgcaacatca accccctgca
      541 aagaactgaa ccccctgatc atcctcgtgg aatttcctga taaaaagcat gtggctgagc
      601 ggaatttctt tgtgaaccgt tacaataccc aggtcaacac ctacctccag tcggtctcgt
      661 acggggcagt ctgcctgaat ggtgaagtga caccaacatg gtacacactc ccccttgaga
      721 ccacctccta ccggatatct tcacgaaacc tggaggtgaa ccggaccagg gtggacaacc
      781 taatccggga tgcactggct gcggcagatg ctgactacaa cttctctgat tatgatttcg
      841 tggtaatcca gctcggggtc cttacgcctg attatggtat ggtcggcctc tgtgggtatc
      901 cgggaatgct cgggtggacc aagaccagtg tcctgaccgc acccagcgga gaggtggtac
      961 aaaggggagt ggccatcttc accgcccagg cccatgtcgg gacgaatttc catgacatcg
     1021 cccatatcct tggcggagta aaagacggaa accggatggt accctgcctc tatgatcatg
     1081 accttcaggc aaagccgggg ccggacctgg aggtgttccg caattccatg atcaatatgg
     1141 ggtattggga tcccatgtcc tgccatttct accggaacga cacttcgccc cccgggatct
     1201 gctcctggac aaggatacgg ctgggatggc tggatgaaga gaagatcctg accgtagatc
     1261 cagacaacca gaccgaagtg atgctcggac ctctcgagga tacgtcctca gaagtacttg
     1321 tcatcaggat acccctctct tcatcgacgt actatcttgt tgagaatcgt gcaccgatcg
     1381 gcgtggacag ggtgctgcct gatcacggga tactcatcat gtttgccgac gatcgtatcg
     1441 cggaatgtca ccatggagaa gctcccgtga aattgatcga tgccaatccc gcgatcccat
     1501 atctcgaagg ggctgcattc gatatcggag agaaggatga atttgtcgat caggcaaata
     1561 acctcagaat caaattgctg gaaaaatcag gcagtgaata ccgtatcctg atagaaccac
     1621 tttaataatt ttaccaagag tgagagtgca cattccgggt tcatgtgcaa cgtatccggc
     1681 caccctcagg ggatattccg gtggttttca ccaaccttct ctgctcttgt gcatatcagg
     1741 gatgccagct cccgcagtat gcaattcaat gaggttctca aaggtggtgc tctttcctgt
     1801 catgatgaat gtgcactcca aaaaaagacg atgggaaaaa accgggaacc gatagaggtg
     1861 atacagcctc cggcaagcag caccgctccc tgttttccga tcccatccaa agtgacatcc
     1921 gctcctccag agtaaaattc ctccatcata aataggagaa cctcatacac cccctgagag
     1981 cgatcctttc atggggaaat cccacatagt ccaatacctg ttcactgcag ggttcattct
     2041 gcttgttctc ctgctcctga tgcacccttc cgcccccgat ctttttccgt cccgtgattc
     2101 ccagccctct gtttcactcc tctatgcctc gtcaggttcc atggcccagc tcctgaacac
     2161 cggccagatc gatgcgtttc ttatctggga accgatcgta gcaaatgccg aattatcagg
     2221 tatcggaaaa cggattgccg ttccttccga tctcccgcct ccgggaaaat gggacaacgc
     2281 tgccatcaat atcctggtgt tacgacagga taccatccgg gcacatcctg acctcgcggc
     2341 actcctttca gccctcacca ccgctgcaat cgaccggaca aacgaggatc ccacccttgc
     2401 agagaacatt acggcacact gggtgtatgg aaaaggccct atccttaccc cccagggaac
     2461 ccttgatccc ctcactgttg agcagcgctc ctttgaaaat atggtgttta cccccgatgc
     2521 tgcacccccg gaagcctcga tcgtcgagta caccatcgat tcaatgacag gtgggaccgg
     2581 cagttatgat ccaatgatgt gggttgacag cacggtactt gcaagagctg catatttttt
     2641 gaatggcacc gctgtgccga caatagatcc ggctcttcca actctcaata taggctacat
     2701 tccctcaagt gacaattatg ccccggtcta tgtcatggtc aaggactcag aatatttctg
     2761 tgaccggtat ggattctgtc tggtcccgga tgacccgtcc ctctcgcggc cggtcacctg
     2821 cactcttctt gtcaacggg
//
LOCUS       DAOOCO010000213         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_35286_length_2826_cov_4.092746, whole genome shotgun sequence.
ACCESSION   DAOOCO010000213 DAOOCO010000000
VERSION     DAOOCO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35286_length_2826_cov_4.092746"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..609)
                     /locus_tag="PK154_09700"
     CDS             complement(166..609)
                     /locus_tag="PK154_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11372.1"
                     /translation="MIEYWIVVTIGVIVVVSALCITYLYAENRELRLVRSRYRLKVYE
                     ELLHAVTDLNVAGADVYKLDMAKRRLAQTLNRLNLVASGDVLKGVNDLLDFLNETPSG
                     NYDVLRQTNILNAIVRAARNDLDPDSAKDLEDTAFRFRFYAPPKG"
     gene            complement(685..1890)
                     /locus_tag="PK154_09705"
     CDS             complement(685..1890)
                     /locus_tag="PK154_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW11373.1"
                     /translation="MKIIKDPVHGYIDVTPCALELLDSPILQRLRYIRQLGFSFLVYP
                     GAHHTRFEHSLGTLYLANVMARQLGLHEEEQNLVAAAGLIHDIGHGPFSHAIEPVMVE
                     FCGRSHHEVSALLDDPDLSECLERHGILQKEVIALLSGNHPLSGIIHGELDVDRMDYL
                     LRDAHYTGAPYGTVDAHRLIRNTCLSEGAITLEEGGINAAESLLIARTLMRPAVYYHH
                     VSRIAEMMLSAAVRSHLEKDIRASPPEFMRLDDAGCMQTLLQSPCAVARALALQIYQR
                     RLYKRAVYVGREQVAPSMTAQKMHQQKRQNISTAIAEDAGVEPHEVLVDIPEFPRSMS
                     VHVRIKNRHRVIGLEEISPLVTTLNETRRGQWRLGIYTPAGHVPAVAAAAREVLQIRP
                     LTTQERLPF"
     gene            1938..>2826
                     /gene="cofD"
                     /locus_tag="PK154_09710"
     CDS             1938..>2826
                     /gene="cofD"
                     /locus_tag="PK154_09710"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HPW11374.1"
                     /translation="MITFLSGGTGTPKLVRGARRHVSDHAITVIVNTAEDIWVSGNHL
                     SPDIDTLLYLFSGQLNTDTWWGITGDSFHTHQELNRLGVDEYIGIGDRDRALHIARGE
                     LLREGYSLTEAIQILSARMGIGACILPMSDEEVATLIRTDKGTLHFQEYWVKHRGTLP
                     IRDVVRYAPTGPFAGAGVIKAIKNADRVIIGPSNPITSILPILECSGVIPALMDSFVI
                     AVSPFIGNAPISGPAAELMNAWGLSPDSASTFSLYKEFCDLFVQDIRDPVDVPGSLRC
                     DTLMTNEEKSAALAETLLSA"
BASE COUNT          653 a          757 c          743 g          673 t
ORIGIN      
        1 aattacgccc ccgcactccc tcatctggtc gataaagccc ctcattccag ccctctcttc
       61 catatcaata cctccggctt ctttaccttc agcttagcga tgctggctta ttaaaaatcc
      121 ggggagatac tctggaaaag aggattattc cgatatctct gtcgttcatc ccttgggggg
      181 agcatagaac cggaagcgaa atgccgtatc ttcgagatcc ttggctgaat caggatcaag
      241 gtcgttcctg gcggctctta cgatagcatt gaggatgttg gtctggcgga gcacatcata
      301 gttccccgaa ggggtttcgt tgagaaagtc gagaaggtca ttcacaccct tcaagacatc
      361 ccctgatgcg accaggttga gccggttcag ggtttgtgca aggcgccgct ttgccatatc
      421 gagtttgtat acatcggcac ctgcaacgtt cagatcggtc acagcgtgta gcaattcttc
      481 gtagaccttc agcctgtacc tgctccgaac caggcggagc tcccggtttt cagcgtaaag
      541 ataggtgata caaagtgcac ttaccaccac aattacgcca atcgtgacga ctatccagta
      601 ttcgatcatg tgtttccccc tcgctggaag attccagctc gatggtgata aacttttttt
      661 tttgatcccg gtggatcgac ccattcagaa cggtaaccgt tcctgggttg taagcggacg
      721 aatctgaaga acctcccggg cagctgcagc aactgctgga acatgcccgg caggagtgta
      781 gatccccagt ctccactgac cgcgccgtgt ttcattcagg gttgtcacaa gaggggagat
      841 ctcctcaagc cctatcaccc ggtgtctgtt cttgatcctg acatggaccg acatggaacg
      901 gggaaattca ggaatgtcaa ccaggacctc gtgaggttcc actcctgcat cctctgcaat
      961 tgccgttgat atattctgtc tcttttgctg atgcatcttc tgtgcagtca tcgatggtgc
     1021 tacctgctcc cgtccaacat agaccgccct tttgtaaagc ctccgctggt atatctgaag
     1081 agcaagggca cgggccacgg cacaggggga ctgcaggagc gtctgcatgc acccggcatc
     1141 atcgaggcgc atgaattctg gagggctcgc cctgatgtcc ttctcgagat ggctgcgaac
     1201 agcggctgac aacatcatct ctgcaatcct gctgacatga tgataataga ctgccggcct
     1261 catgagggtc cgtgcgataa gaagggattc ggcagcatta attcctcctt cttcaagggt
     1321 aatggctcct tcagacagac aggtgttcct gatgagccgg tgagcatcga cggtcccata
     1381 tggagcacct gtgtaatgcg catcgcgcag gaggtaatcc atcctgtcca catccagttc
     1441 cccgtgaata attcccgaaa gggggtggtt tcccgaaaga agagcaataa cctctttttg
     1501 aagtatcccg tggcgttcga ggcattcgga tagatctggg tcgtcaagaa gcgcggaaac
     1561 ctcatgatga gatctcccgc agaactccac catgaccggt tcgatggcat gcgagaacgg
     1621 accatgtccg atatcatgaa taagtcctgc tgcagcgacc aggttctgct cctcttcatg
     1681 gaggcccagc tggcgcgcca taacgttggc gaggtaaaga gtaccaagcg agtgctcaaa
     1741 ccttgtgtgg tgtgccccgg gatataccag aaatgagaaa ccgagctggc ggatgtaccg
     1801 aagtcgctga agtatggggg agtcgaggag ttcaagagcg caaggggtaa catcgatgta
     1861 cccatgcacg ggatccttga tgatcttcat ctggcaccta aacagtcttg ggatagagca
     1921 gataaaacta ttgagtgatg atcacatttc tctccggagg gacaggaacc ccgaaactgg
     1981 tccggggggc acggaggcat gtgtccgacc atgccattac cgtcattgtc aatacagccg
     2041 aagatatctg ggtatcggga aatcacctct cccctgatat cgataccctc ctctacctct
     2101 tttccgggca gctcaatacc gacacctggt gggggataac gggggattct ttccataccc
     2161 accaggaatt gaaccgtctg ggggttgacg aatatatcgg aatcggtgac cgggatcgtg
     2221 cactccatat cgcccgcggc gaactactca gagaaggcta ttcacttact gaggctatcc
     2281 agatcctctc cgcccggatg ggtattggag cctgcatcct tcccatgagt gatgaggagg
     2341 tagcgaccct gataaggacg gataagggta ctctccattt ccaggagtat tgggtaaagc
     2401 acaggggaac tctcccgatc cgggacgttg tccggtacgc accgacaggg cctttcgcag
     2461 gcgcaggggt tatcaaggcg atcaagaatg ctgatcgcgt cattatcggg ccgagtaacc
     2521 ccataacaag tatcctgcca atcctcgaat gttctggcgt cattccggca ctcatggatt
     2581 cttttgtcat tgcggtcagt cctttcatcg ggaacgcccc tataagcggt ccagccgcag
     2641 agctgatgaa cgcctggggc ctttcccctg attcagcttc aacattctca ctctacaagg
     2701 agttctgtga tcttttcgta caggacataa gagatccggt tgatgtaccc ggaagtctcc
     2761 gttgcgatac actcatgacc aatgaggaga agagtgcggc tctcgcggaa accctgcttt
     2821 cagcgg
//
LOCUS       DAOOCO010000214         2800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_35789_length_2800_cov_3.271767, whole genome shotgun sequence.
ACCESSION   DAOOCO010000214 DAOOCO010000000
VERSION     DAOOCO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_2800_cov_3.271767"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PK154_09715"
     CDS             complement(<1..983)
                     /locus_tag="PK154_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPW11375.1"
                     /translation="MAPEKGRWVLVILGMVINLCLGTLYSWSVFVEPLIAYFSLELGQ
                     AVTVNDVLMPFSVFIAVFAITMLLTGRYVEELQPRMVTMIGGIFCGLGWLLASTATSL
                     MMLTLVFGVIGGIGVGIAYGATIAVAARWFPDRRGLAVGMTVLGVGFSAVLTANLAGF
                     LIDAYDVMTTFLFFGIGILLITVPMALPLTFPPSGWRPRGWKPPVHGERGHVACDCNR
                     AEMVRTPAFYGLWLCYFIGSVAGLMAVSIAKPVGTELVHIETGLATTLVAFFALFNGG
                     GRPVFGVLTDRINPRNTAMLSFVLIGLAGLVMWQWPVPAGYIVAFALLWGCL"
     gene            complement(1490..2350)
                     /locus_tag="PK154_09720"
     CDS             complement(1490..2350)
                     /locus_tag="PK154_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW11376.1"
                     /translation="MNDNAAILVLGALIVGILVAGCTTPSPQTVPVTPVPTTAMPVTM
                     PEETTLTGTWTLVSILAQGRGTSMVPLTTITTTFTESGLAFGSAGCNRYVVTYQVSGD
                     RIAVGKPAVAKNECSSPIGVSDQETIYLSNLKAAEHFTIDGERLTFYDLKGTVLLIYD
                     RSAPQESSLPIAGIPWILQLYRMESGANMPANLDTEVTALFGTDETLTGSAGCNAYNG
                     TYTTSGGTTISISEPLVTTEKYCIAYGVMDQEEDYLALLPSVASYEVTSDGMLYLRDE
                     HSTPLIWYSI"
     gene            2548..>2800
                     /locus_tag="PK154_09725"
     CDS             2548..>2800
                     /locus_tag="PK154_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11377.1"
                     /translation="MKRRKMAMSSLDKPIVDLAIGVNHRILALWAADCAERVLPLFEE
                     LCPGDPRPRRAIEALREWVRSGVFRMADVRRTALDAHAAA"
BASE COUNT          645 a          751 c          799 g          605 t
ORIGIN      
        1 aggcatcccc agagcagggc gaaggcaacg atataccccg caggtaccgg ccactgccac
       61 atcacgagac cggcaagtcc gatcaggaca aatgagagca tggcggtgtt cctgggattg
      121 atccggtcgg taagaacccc gaataccggc cgccccccgc cattgaagag ggcaaagaac
      181 gccacgagag tcgtggccag ccctgtctca atgtgtacca gttcggtccc gacaggtttt
      241 gcgatcgaga ccgccatcag gccggcgaca ctgccgataa agtagcagag ccagaggccg
      301 taaaaggccg gggtcctgac catctccgct ctgttgcagt cgcacgccac atgccctctt
      361 tcaccgtgaa ccgggggttt ccagcctcgc ggtctccacc cggaaggggg gaaggtgagg
      421 gggagcgcca tcgggaccgt gatcaggaga attcctatgc caaagaagag gaaggtggtc
      481 atgacatcgt aagcatcaat gaggaaccct gccaggttcg cggtcaggac cgcagagaag
      541 ccgactccaa gcaccgtcat cccgactgca agccctctcc tgtccgggaa ccaccgtgca
      601 gcaacagcaa tcgttgctcc ataggcgatg ccgacaccga taccaccgat caccccgaat
      661 acaagggtga gcatcatcag ggaggttgca gtcgaggcca gcagccagcc gagcccacag
      721 aagattcccc cgatcatggt aaccatccgc ggctggagtt cctcaacgta tctgccggta
      781 aggagcatcg tgattgcaaa aaccgcgatg aataccgaga atggcatcag cacatcgttg
      841 accgttactg cctgtccaag ttccagggaa aaatatgcga tgaggggctc gacaaagaca
      901 ctccacgagt aaagggtgcc gaggcagagg ttgatgacca tgccaaggat cacaagcacc
      961 catctccctt tttccggggc catgtcgaag gggaaccggg gttcgccagg attgcctcgt
     1021 tcctttgtat ggagtgccat acaggtgagc cgttggtgac gggaccagat aacgatatcc
     1081 ctggaatatg tttccccctg gtccccggcc gttcttcctg tcatgccctg ccctccgaaa
     1141 gggaagggat cattcagtgt tcagaaggag tgttgagaag gggtcatccg gattccagga
     1201 tcctgtccct ttctcataga aggtttgagg cagaattgag tatcccgaac taatccagaa
     1261 acctgttgtt ccaaaaaaag catggtcgtt ctcgagcatc gtcctgcacg acaccataaa
     1321 aggtgctgaa agatgaaaac ctgcacgccc ttccttaccc tgacttcctt gttcaggaca
     1381 gcatgctttc ttttgaacag cctttggaag atgccagaca ccttccctgt gatggtatag
     1441 tgcgatctgc aggtggggtc ctcgaaggaa aaaatgagag ggtccctttt cagatcgaat
     1501 accaaatgag cggggtggag tgttcatccc tcagataaag catgccatcg ctcgttacct
     1561 cgtaggatgc caccgaaggc aggagggcaa ggtagtcctc ctcctggtcc atgacaccgt
     1621 atgcaatgca atacttctcc gttgttacca gtggttcgct gattgagatc gtggtccccc
     1681 cgctggtggt gtacgtgccg ttatatgcgt tacaaccggc cgatcctgtg agggtttcat
     1741 cagtaccaaa gagggcggtt acctccgtat ccaggttggc aggcatattc gctcccgatt
     1801 ccattcggta cagctggagg atccagggaa tacctgcgat aggaagagaa gattcctggg
     1861 gtgctgaccg gtcgtagata agaagaacag tccctttgag atcatagaag gtgagacgtt
     1921 cgccatcgat ggtaaaatgt tctgctgcct tgagatttga caggtagatc gtctcctggt
     1981 ccgatactcc gatgggactg ctgcattcgt tcttcgcgac cgcaggttta cctaccgcga
     2041 tcctgtcgcc gctgacctgg taggtgacga cataacggtt gcaccctgca gatccaaagg
     2101 ccaggcccga ttcggtgaag gttgtcgtga tggtggtcag agggaccatg ctcgtccccc
     2161 tcccctgggc aaggattgag acaagtgtcc aggtcccggt aagggtggtc tcctctggca
     2221 tggtgaccgg catggctgta gtgggcaccg gggtgaccgg tactgtctgc ggagatggtg
     2281 ttgtgcagcc agcaacaagt attccgacaa tgagagcacc aagaacaagg atagcagcat
     2341 tatcgttcat gggtaacagc ttatcgccgt ctcttaatag tatttctctc ttttgcggcc
     2401 aatctcccgg atattaggat ggataaaggc cctggcagcg gatagccgtt gttcctgaca
     2461 ccggatactt cttgggatca ggtttttgcc cgggtgatga taatctgatg ctgacggttc
     2521 ccattccggc agtagggtat tgatgccatg aagcgacgga agatggcaat gagcagcctt
     2581 gacaaaccta ttgtggatct tgccattgga gttaaccata ggatccttgc actctgggct
     2641 gctgattgtg cagaacgagt cctccccctc tttgaagaat tatgtcctgg agaccctcgt
     2701 ccccggagag cgatagaagc actccgggag tgggtccgta gcggggtgtt ccggatggcg
     2761 gatgtgcgga gaactgccct tgacgcccat gctgcagccc
//
LOCUS       DAOOCO010000215         2775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_36266_length_2775_cov_3.048529, whole genome shotgun sequence.
ACCESSION   DAOOCO010000215 DAOOCO010000000
VERSION     DAOOCO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36266_length_2775_cov_3.048529"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK154_09730"
     CDS             complement(<1..298)
                     /locus_tag="PK154_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11378.1"
                     /translation="MERIARHCSRNGTIPYSFSLFCALKAEAGLFPGTNSVIVTVVGA
                     SFSGRRLDLAGGGVRWGSLTIGIRFVILSSHRRAKSQKTRAISSTLIGLTRDSIF"
     gene            362..1555
                     /locus_tag="PK154_09735"
     CDS             362..1555
                     /locus_tag="PK154_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPW11379.1"
                     /translation="MQTKVPLSELEGRMKRFRVLMDMHHPDWEIAFITSNINLYYFTG
                     TIQDGILLIVRAGEASFWVRRSFERAVDESLFPHILPMRSYRDPAGTMTAIPGIVYLE
                     SEQVPLALFQRLQKHFSFREFRDIDRQILQARSVKSPYELDLMEQAGTIHRHVLEDLA
                     PEVLYEGMSELEFTTKLYSLMVNEGHHGIVRFGMSESEILLGLIGFGESSLYPTYLDS
                     PGGNRGISPAVPLMGSRNRYLRDGDLVFVDIGCGVQGYHTDKTMTYVFGRALPEDAVA
                     IHQQCVDIQNAIIPLLKPGAIPSQIYEKIMTSLEPGFLENFMGYGKRNVQFIGHGIGL
                     EIAENPVISSGFDEPLESGMVIAIEPKKGIKGIGMVGIENTFVVTPDGGRSITGCHPG
                     LMPVG"
     gene            2095..>2775
                     /locus_tag="PK154_09740"
     CDS             2095..>2775
                     /locus_tag="PK154_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11380.1"
                     /translation="MKIPYPKKKMEAVRMITLKRSVTAVGLKRSILVFMVVFLLVPFS
                     AQAGQLQVPGVDIRSMGSSLGFQQASDLPDLSGLAWLGGDCFLAVHDAKIDGESGNPR
                     GSLLYVPADSKGILWKPQALSFHGLKSNDLESAARIPGTKDVLLLESGDSLGDPRFQK
                     IFKAHVEGNRVRIIDVIPWPVPVHNVEAAAVAALGDRYVFLYAERAQSLPTTDVRWRR
                     AQASTPDLA"
BASE COUNT          651 a          700 c          757 g          667 t
ORIGIN      
        1 gaaagattga atctcgggtc agaccaatca gggtcgagct gatggccctc gttttttggc
       61 tcttcgctct cctgtgtgag gaaagaatga cgaaccggat acctattgtg agactgcccc
      121 acctcacacc cccacctgcg agatcgagtc gccgccccga aaaggacgct ccaacgacgg
      181 ttacaatgac tgaattggtt ccagggaaca accccgcttc agcctttaac gcacaaaaca
      241 gcgagaagga gtagggtatt gtgccattcc gcgaacagtg ccttgcgatt ctctccacgg
      301 gaacaacaat tcccttgtcg tatcattagc cgtaaatagt tacaattgca gatagttcag
      361 gatgcagacc aaggtccccc tcagtgaact cgagggaagg atgaagcgtt tccgggtcct
      421 gatggacatg caccatcccg attgggagat tgcattcatc acctccaata tcaatctcta
      481 ttatttcacc ggtactattc aggacgggat actgctcatt gtacgggctg gtgaggcctc
      541 cttctgggtg cggcggagtt ttgaacgggc agtggacgag tccttattcc ctcatatcct
      601 ccccatgcgg agttacaggg atcctgcagg aacgatgacg gctattcccg gaatcgtcta
      661 cctggaatcc gaacaggtcc cgctcgcgct cttccagcgt ctgcagaagc atttttcatt
      721 cagggaattc agggatattg accggcagat cctgcaggca cggtcggtga aaagcccgta
      781 tgaactggac cttatggagc aggccggcac aatccaccgt cacgtgctgg aagaccttgc
      841 accggaagtc ctatatgaag ggatgagcga actggagttc acgaccaaac tgtattctct
      901 catggtgaac gaggggcatc atggtatcgt ccggttcggt atgtccgagt ctgagattct
      961 cctggggctg atcgggtttg gtgagagttc cttatatccc acctatcttg acagccccgg
     1021 ggggaacagg ggaatatcgc ctgctgtccc gctgatgggg agcaggaacc ggtatcttcg
     1081 tgacggggat ctcgtctttg tggatatcgg gtgtggagtc cagggatacc acactgataa
     1141 gacgatgacc tatgtgttcg gacgggcatt gcctgaggac gcagtcgcaa tccaccagca
     1201 gtgcgtggat atccagaacg cgatcatacc cctgctcaaa cccggggcaa tcccgtcaca
     1261 gatctacgag aagatcatga caagcctgga gcctggattc cttgagaatt tcatgggata
     1321 cgggaaaagg aacgtccagt tcatcggtca tgggatcggt cttgagattg cagaaaatcc
     1381 ggtcatcagc agtggatttg atgaacccct ggaatcaggg atggttatcg ctatagaacc
     1441 caagaaaggg atcaagggta tcggcatggt tggtatcgag aatacctttg ttgtcacccc
     1501 ggacggcggc aggagcatca ccggctgcca tcccgggtta atgcctgtgg gatgaaactc
     1561 ttaattttca aatggctctt cgctctctgt tgtggggaaa ttgatgagat ggatacattt
     1621 tgtggggttg gccgctcccc ctacccccca cctgcgatat atgccgccaa accaggaggg
     1681 cgcccaggtg gcggatcagt ggccatttac atcaataggg aaaacagcgt tacttcagga
     1741 tatgccttga cgttgcacta aatatggtgc attgagtttt ggaaccaatt cgtatcattt
     1801 ttcaattgat aggatcttgg ctgatgtaca ggatcgcggt tctcgttttt accgcaattt
     1861 ccttacacaa ccttttatcc aaaatgccgc aaccagagaa ttccaggata ggaccctatt
     1921 gcctatttat cccggacagc tgatgttgaa aaacgggtta atgctgattc cgtgtagcac
     1981 tccagcactt cacaggatca tgtaaggccg gtgagtaaga gaatttcgtc tctctgttgc
     2041 actcggcttt tttttgatgg tgccagaata aatgtaggga gagagagatc agacatgaag
     2101 ataccgtacc caaaaaaaaa gatggaggca gtacgcatga tcacattaaa aaggtcagta
     2161 accgcagtgg gattaaagcg atcaattctt gtgttcatgg tcgtttttct tctggtgccg
     2221 ttttccgcac aggctgggca gcttcaggtt cccggagtcg atatccgcag catgggcagt
     2281 tcgctgggat ttcagcaggc atcagatctc cccgatctca gcgggcttgc ctggctcggg
     2341 ggagattgct ttcttgcggt ccatgatgca aagatcgatg gcgaatcggg aaacccgcgt
     2401 ggcagtctgc tctacgtacc cgccgactcg aaggggattc tttggaaacc acaggcgctc
     2461 tccttccatg gactgaaaag caatgacctc gaaagcgctg cacgtattcc cggaacgaag
     2521 gacgtgctgc tcttggagag cggagatagc cttggggatc caaggttcca gaagatcttt
     2581 aaagcgcatg tggagggcaa ccgggtgcgg atcattgatg tgataccatg gcctgtcccc
     2641 gtccataatg tcgaagctgc tgctgttgcg gcgctcgggg atcggtatgt atttctctat
     2701 gccgaacgcg cccagagtct tcccaccacc gatgtgcgct ggcgccgcgc ccaggcttcg
     2761 actcccgacc tcgcc
//
LOCUS       DAOOCO010000216         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_36330_length_2771_cov_7.148380, whole genome shotgun sequence.
ACCESSION   DAOOCO010000216 DAOOCO010000000
VERSION     DAOOCO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36330_length_2771_cov_7.148380"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK154_09745"
     CDS             <1..691
                     /locus_tag="PK154_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW11381.1"
                     /translation="VQDAYPEKVPLDLMPVYVWNIPLDIVILGLVSVFVPVLFVPMQL
                     VFSLLAWLHFGHKRVLRNNVLENETRNAVYLCIRDNPGINTSSLSQLLGMNIGTLRYH
                     VELLCRMGKVVPEPNSRGMRYYVDTGTCPGLERKVAGYLNEQAKSRILNVVLQHPGST
                     RKEIAARLIMSGANVTWHMKPLLRDGIVRDEKRGRFVRYYICADAKECVQAHGSGKPA
                     ISGERVPGTGI"
     gene            964..2589
                     /locus_tag="PK154_09750"
     CDS             964..2589
                     /locus_tag="PK154_09750"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPW11382.1"
                     /translation="MVSEQVTSKSFTKRLDHLGIIAALVEGLGIVDLVNARLPKLRHH
                     TLTHGDIVLALILNGLGFTQRRLYLFAAWFENKPLKRLFRKEVTAADFTDDVIGRTLD
                     AIKEYGPERLYQEIISQIFLVTQLGYHQVHLDTTNFSVHGAYDTSDPHPTIRITQGYP
                     KDGRWELNRFGLGLIVNQVGVPLFMKLLSGNSDDKVALPEMLARFRSQVAFPEPLYCI
                     ADSAFYTGDSLKKLDEKTFFVTLVPGTIGEQQALLRRDLPLTPMADPRYSFFETESHY
                     GGVHQKWVVYRSAANHAKQEKTLEKRRSKEDEKVKKALIHLNTREFACEADALAEAER
                     WIRTYPFYEFESVSVVTQRRRAPGRRGRPKKDEVTETVYLLVAAIRPNEAAWAAEQAA
                     LGRFVLATNDLNLSAKDLLAQYKNQDVVERGFRFLNDSSFAISDVFLKKVGRIEALGM
                     MMTLMLAVYSLGEYQLRKRLEQLGKTVPNQTGKPTARPTLKWVMTFFDGVVESHRYDP
                     STDTVWCEGILNMSKRCWDVVEAFGPICEKYYS"
BASE COUNT          653 a          712 c          759 g          647 t
ORIGIN      
        1 cgttcaggac gcatatccgg aaaaggtgcc gttggatctg atgccggtgt acgtctggaa
       61 tatccctcta gatattgtca ttctcggact cgtatccgta ttcgttccgg tgctctttgt
      121 cccgatgcag ctcgtattct cgctgcttgc atggctgcat ttcggccata agagagtcct
      181 gcgcaataac gtccttgaga acgaaacccg gaatgcagtg tacctatgca tacgtgataa
      241 tccgggaatc aatacaagtt ctctctccca attgctcggg atgaatatcg gaaccctccg
      301 ctaccacgta gagctgttat gcaggatggg aaaggtcgtc cccgagccga acagcagagg
      361 catgagatat tacgtcgaca cgggcacctg tcccggtctg gaacgaaaag tggcgggata
      421 tctcaacgag caggcaaaga gccggatact caatgtcgtt ttgcagcacc cgggaagcac
      481 gagaaaagag atcgcggccc ggctcattat gtcgggcgcg aacgtcacct ggcatatgaa
      541 accgctgctc cgggacggta ttgtcagaga cgagaagaga ggacggttcg tacgttacta
      601 catctgcgcc gatgcgaaag agtgtgtaca agcccacgga tcggggaagc ccgcgatctc
      661 cggggagcgt gtgcccggca ccggtatctg attgccgttc gatagttctc tataactgca
      721 ccgtttgccc cttttcccgg atcatcccgc ggcttatcga ggatgcgttc agagtctccg
      781 ggcgccgcct gcacgtcatg catggacaac ggagtcggcc cgggcgatct cccctacttt
      841 ccgcggtaac aatcctcact ttctgaatta atcaactgca tcaagctaac ctcaagctag
      901 ggcattatat atttaaatat ttatacaagc attattaatt aacttcttcc atatttaact
      961 gggatggtat cagagcaggt tacctctaaa tccttcacca aacgtctgga tcatcttggg
     1021 atcattgctg cacttgttga aggacttggc atcgtcgatc tggtcaacgc acgactgccg
     1081 aaacttcggc atcacaccct gacccatggt gatatcgtcc ttgcgctcat cttgaacggt
     1141 cttggtttca ctcagcgtcg actctacctg tttgccgcct ggttcgaaaa caaacccttg
     1201 aaacgccttt tcagaaaaga ggtcactgct gccgatttca ccgatgatgt cattgggaga
     1261 acgctcgacg ccatcaagga gtatggcccg gaacgcctct accaggagat catcagccag
     1321 atcttcctcg tgacccagct cgggtatcat caggtgcatc ttgacaccac caacttcagc
     1381 gtccacggtg cctacgatac ctctgaccct catcctacga tccgtattac gcaaggctat
     1441 cccaaagacg gacgatggga gttgaaccgg tttggactgg gtctcatcgt gaaccaggtt
     1501 ggtgtcccgc tcttcatgaa actcctttcc gggaacagcg acgataaggt tgctcttcca
     1561 gagatgcttg cccggttccg gagccaggtc gcgttcccgg aaccccttta ttgtatcgct
     1621 gattctgcgt tttataccgg ggattccctc aaaaaactcg atgaaaagac gttcttcgtt
     1681 actctggtac caggaaccat cggtgaacag caggcgctgc ttcgccggga tcttcccctc
     1741 actccgatgg ccgatccccg atactcgttc ttcgagaccg agtcccatta tggtggcgta
     1801 caccagaaat gggtggtgta ccgttctgcc gcaaaccacg cgaagcagga gaagaccctg
     1861 gagaagcgcc ggtcaaagga ggatgagaag gtgaagaaag cccttatcca ccttaatact
     1921 cgtgaatttg cctgtgaggc agacgctctc gctgaggctg agcgctggat ccggacctat
     1981 cccttttacg agtttgagtc tgtttccgtt gtcacccagc ggagacgggc tcctggccgc
     2041 cgggggcggc cgaagaagga tgaggtgacc gagacggtgt atttgctggt agcagccatc
     2101 aggccaaacg aggctgcctg ggcagcagaa caggccgctc tgggcagatt tgtccttgct
     2161 accaatgatc tcaacctctc tgcaaaagac ctccttgccc agtataagaa tcaggatgtg
     2221 gttgagcgcg ggttccgctt cttgaatgat tcgagttttg cgatatctga tgtattcttg
     2281 aagaaagtag gtcgtatcga ggccctggga atgatgatga cgctaatgct ggcggtatac
     2341 tcattgggag agtaccaact gaggaaacgg ttggagcagc tcggtaagac tgttcccaat
     2401 cagacaggga aaccgacagc acgacccacg ctcaagtggg tgatgacatt tttcgatgga
     2461 gtggttgaat cccatcgata cgatcccagt acggatacag tatggtgtga agggatcctc
     2521 aacatgagta aacggtgttg ggatgtcgta gaggcgtttg gaccgatctg tgagaaatac
     2581 tactcgtaag gctgattcct gaaggatcgg agagggagga gggaaaggcg agtcccacgg
     2641 attggagctc cggctgtgga gaggcaggga gctacgacga ggggggacag gtccatgggg
     2701 aggataacaa cctggttttc ttgaaaggag ggagaaattg cgtgatcgaa aactttaccg
     2761 cggaaagtag g
//
LOCUS       DAOOCO010000217         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_36633_length_2757_cov_4.674685, whole genome shotgun sequence.
ACCESSION   DAOOCO010000217 DAOOCO010000000
VERSION     DAOOCO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36633_length_2757_cov_4.674685"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..728
                     /locus_tag="PK154_09755"
     CDS             60..728
                     /locus_tag="PK154_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW11383.1"
                     /translation="MLSCDLHVHTIFSRDGESTPLEIIRRAEAVGLDAIAITDHDTVE
                     GAIAALSIPTSLVIIPGIEISTRQGHLIALGVTEKIPSGLDVIETIHLAKERGAILIL
                     PHPYHVWRHGVGRKLRSALYSVDAIEVFNSRYIVGSANLKAARMARRICKPCVGGSDA
                     HHARYVGFGRTTIDAKKDCGSILAAIREGKTSAAGKMTPLPSYTRQTIRNTWKKFRRR
                     ISHR"
     gene            692..1519
                     /gene="truA"
                     /locus_tag="PK154_09760"
     CDS             692..1519
                     /gene="truA"
                     /locus_tag="PK154_09760"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPW11384.1"
                     /translation="MEEVQAPDLPPVRLACRVAYLGDRFFGSQMQAAERTVEGEFVAA
                     CTRLGLFSDYRKAGFVAAGRTDRGVHARGQVFSFSTIYPSRALSALNWQLPLDCWISG
                     YAKVDPGFHPRYDAKGRTYRYYFGTTHLDEKAMDSAAHRFVGTHDFSVFSRTSERNPE
                     RTITSARVWCDENTCVFEVTGDSFLWNMVRLMAASLLRIGQGLEGEERIVQRLDGERN
                     SPLSPLPPDGLILWEVNCGITFNPLPIEGKRQGFLVRLREHHTALERVVRDLNEGDR"
     gene            2031..2489
                     /locus_tag="PK154_09765"
     CDS             2031..2489
                     /locus_tag="PK154_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW11385.1"
                     /translation="MAPFACTRCGKCCISLGRHIRIERSLSPVQYYCRVAVSGELLPV
                     SVQPELRELFASGDQDPAWCPFLRKDAAGLFTCTIYQTRPRICREFRCRTMIIYDQCG
                     REAGYVKGRRTLVSGDAGLEAVWQKIPSGADDTSLKGILRNHGYRAESLD"
     gene            2565..>2757
                     /locus_tag="PK154_09770"
     CDS             2565..>2757
                     /locus_tag="PK154_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit beta"
                     /protein_id="HPW11386.1"
                     /translation="MTEIPKEEFILKCTSACAGCPSTLILRYVLKAAGADTVLVVPAC
                     CTSVIQGIYPNTAVNVPVYN"
BASE COUNT          649 a          768 c          741 g          599 t
ORIGIN      
        1 gggaggagga tgcgcataag tttccttaat accagtgaga taaaaagtca tatgatagca
       61 tgctctcctg cgacctccat gtccatacca ttttttcacg agacggggag agcacccccc
      121 tggagatcat acgcagagct gaggcagtcg gcctcgatgc gatcgcaata acagaccatg
      181 atacggtaga aggtgccatt gccgcactca gcatccccac gagccttgtt atcatcccag
      241 gaattgagat ctcgacgcgc caggggcacc tgattgcact cggtgttact gaaaagatac
      301 cctctggact ggacgtgatc gagaccattc acctcgcaaa agagagaggc gcaatcctca
      361 tcctccccca cccgtaccat gtctggcgac acggtgtcgg gagaaaactc cgctctgcgt
      421 tatattcggt cgatgctatc gaggttttca acagcaggta cattgtcgga tcggcaaacc
      481 tcaaggcagc acggatggcg cggcgtatct gtaagccctg cgtgggagga tctgatgccc
      541 atcatgcacg gtacgtcgga ttcggcagga ccaccattga tgcaaagaag gactgtggat
      601 ccatccttgc cgcgatacgg gaaggcaaga ccagtgccgc cggcaagatg acacccctcc
      661 cgagttacac ccgccagact atcaggaata catggaagaa gttcaggcgc cggatctccc
      721 accggtaagg cttgcgtgca gggtcgccta tctcggtgac cggtttttcg gatcccagat
      781 gcaggcagca gagcggaccg tggaggggga gttcgtggcc gcatgtaccc ggctcggcct
      841 cttctctgac taccggaagg caggattcgt tgccgcgggg aggacggacc gaggggtcca
      901 tgcaaggggg caggtctttt cattttccac tatatatccc tcgcgggcac tctctgccct
      961 gaactggcag cttcccctgg actgctggat cagcggatat gcaaaggtcg atccaggatt
     1021 ccatccgaga tatgacgcaa agggacggac ctaccggtac tatttcggga cgacccacct
     1081 tgatgagaaa gcgatggact ccgccgccca tcgtttcgta ggtacacacg acttctcagt
     1141 tttttcaagg accagcgaac gaaatcccga gcggacgatc acctctgcga gggtctggtg
     1201 tgatgaaaat acctgtgtat ttgaagtgac gggtgacagt tttctctgga acatggtgcg
     1261 actcatggcc gcatcgctcc tgcgtatcgg gcaaggattg gagggtgagg agaggattgt
     1321 gcagcggctt gatggtgaac ggaactcgcc actctctcct ttaccgcccg atggcctcat
     1381 cctctgggag gtgaactgcg gaatcacctt caatcccctc cctatcgagg ggaagcggca
     1441 gggatttctc gtccggctcc gggagcatca tactgcactc gaacgggtgg tccgcgacct
     1501 gaacgaaggc gaccgatgaa gcctgttggt aacggagccg atgaaagtga aaattggtat
     1561 gagagagatc cttcattcac acatagacgt aactccgcat gttggcctaa gagaggaaag
     1621 acagcaaggg atcagtcctc tatatctgca ggctctcctc tcagaattac cctgtccatc
     1681 atccccagtg atgctatagg ggtcagtcag agggaacaga ggcataggaa caaccttcct
     1741 gaacgctgtg tacactttca catcccctga tccgttcatg cacaccccct gcaatcgtca
     1801 atgcacttct cgcagatgct atcctctcaa ggctcaaaga acagaataca gcgtaccagg
     1861 aaagctgcca gctcacggct tatccatgca cgaagcggtg cctcttccct gcatgcaaac
     1921 gcgcagagac gatgaccttc atccctggat tcttcacaga gacctcccag ccgacgaact
     1981 ctggatgacc ctccatcttc atcacccaat ggagtttatc ttacagggag atggcaccgt
     2041 ttgcctgtac gagatgcgga aaatgttgca taagccttgg gagacatatc cggatcgaga
     2101 ggagcctctc ccccgtgcag tattactgcc gcgttgccgt tagtggtgaa ctgctgccgg
     2161 tatcggtcca gccggaactg cgggaactct tcgcatcagg tgaccaggat ccagcctggt
     2221 gcccgttcct caggaaagac gctgctggcc tcttcacctg cacaatttac cagacacgtc
     2281 cccgcatttg cagggaattc cggtgcagga cgatgataat ctatgaccag tgcggccgcg
     2341 aggcagggta tgtcaaagga aggaggaccc ttgtatcagg cgacgccggc cttgaagccg
     2401 tatggcagaa gatcccttca ggtgcggatg atacctccct caaagggata ctccggaacc
     2461 atggataccg ggccgaatcc cttgattaaa agcattcatc cccaggaatt accagaatac
     2521 tacgcttatt actgatggaa agctaacctt tttcacgatt ttccatgacc gagatcccca
     2581 aagaagagtt tatactgaag tgcacttcgg cttgtgcagg gtgcccctct accctcattc
     2641 tccggtatgt actcaaggca gcaggagctg ataccgtgct ggtggtccct gcctgctgca
     2701 ccagtgttat ccagggtatc tatcccaata ctgcagtgaa tgttcccgtg tataatg
//
LOCUS       DAOOCO010000218         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_36652_length_2756_cov_4.436875, whole genome shotgun sequence.
ACCESSION   DAOOCO010000218 DAOOCO010000000
VERSION     DAOOCO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36652_length_2756_cov_4.436875"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK154_09775"
     CDS             <1..639
                     /locus_tag="PK154_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPW11387.1"
                     /translation="RPGIGATASILSQYLQELDISVDKQVATGLLYGIRSDTRDFSRN
                     VTPQDLINAAFLLPLTDNSLLDQIMSPSLSEETLDILGTAIANRKIQSGYLFSNVGFI
                     RNRDALPQAADLLITLEGVNTALIYGISDEAIIMSARNRDVRLHIGNVLKEAFGTMGE
                     AGGHPTMAAAVIPLSFFSLAKSKVDLLSLVTEPLLGRFANIVGLESEVRNGI"
     gene            629..1249
                     /locus_tag="PK154_09780"
     CDS             629..1249
                     /locus_tag="PK154_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPW11388.1"
                     /translation="MEFEEWEPYYQEILGYFGITREDDERAARILASLIEKDDCSLLS
                     SLCRGRIVTVCGNAPALPDETDHIKGSIFAADAAAGVLYESGIRPDAVFTDLDGAAES
                     FLEMNREGTIMVIHAHGDNIPQLQYWVPLFPGPVVGTTQSQPVPPLHNFGGFSDGDRA
                     AFAADALGAERIVFVGFDLDDMNVGPMKRGKLLWARRLLRLIGYEC"
     gene            1239..>2756
                     /locus_tag="PK154_09785"
     CDS             1239..>2756
                     /locus_tag="PK154_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW11389.1"
                     /translation="MNADAFIIDGYVDEPACLGVPPYISPYIRTIAGVFTAHGIEPRY
                     ITIDQVRANPRILTPVPGGIAIMIAGVTVPGKYLGGTPATLTEIQQVGTQLRGMNRLI
                     TGPVIFGYSRGGGERAVRKTISGFDHLLTGSPAESLDNWLSGGTTPGDHSYQRSDLWA
                     VTGSSIIRQHPSFPHIMCEVETARGCTRQVTGGCSFCTEPFYGHPRYRTIEGIHHEIS
                     ALYAAGARHFRLGRQPDLLTYGAGQGEFPRPRPELIGELFQTIRSAAPGLQTLHIDNI
                     NPGTLARHPEAGRKALEEIVRYHTPGDVAAFGMETADPIVVRTNNLKADPDEVMEAIR
                     IVNEVGGDRKEGIPDLLPGLNFIAGLAGETKRTFLLNEQFLSRVLASGLLVRRVNIRQ
                     LMPFEGTRAYDDNTLGKHTSQFRAFKEYVRKKFDLPMLRQVYPIGTILHNVIIEEEGA
                     LSFGRQMGSYPILMGIPVHLKRHTVLDTVVVDHGMRSLTGLPVPVQVNLLPSPALK"
BASE COUNT          612 a          774 c          743 g          627 t
ORIGIN      
        1 aggccaggaa ttggggcaac cgccagtatc ctctcccagt atttgcagga gctggatatc
       61 agtgttgaca aacaggtagc caccggtctt ctctacggca ttcggtcgga tacccgcgac
      121 ttttcccgga atgtcacccc ccaggatctg attaacgcgg cctttctcct cccccttacc
      181 gacaattcac ttctcgacca gatcatgtca ccctcgctct ctgaagaaac gctcgatatt
      241 ctcggaaccg cgatcgcgaa caggaagatc cagagcgggt atctcttctc caatgtcggt
      301 tttatcagga acagggatgc acttccccag gcagcagacc tcctgataac tctcgaaggt
      361 gtcaacacgg cgcttatcta cggcatctca gatgaagcga tcatcatgtc agcccggaac
      421 cgggatgtcc ggctccatat cggcaacgtt ctcaaggagg catttgggac gatgggggaa
      481 gcaggggggc acccgaccat ggccgcggcc gttatccccc tctctttttt cagcctcgcc
      541 aaatccaagg tggatctcct ctcccttgtc acggaaccac tcctgggcag gtttgcaaac
      601 attgtcggcc ttgaaagcga ggtcagaaat ggaatttgag gaatgggagc cctattatca
      661 ggaaattctt ggatattttg gtatcacaag ggaagatgac gagagggctg cccgcatcct
      721 cgcgtccctg attgagaagg atgactgcag cctcctctca tccctttgca ggggccggat
      781 cgtcacggta tgcggaaatg cccctgccct ccctgatgaa accgatcata ttaaagggag
      841 catctttgca gccgatgcag cagctggtgt cctgtatgag tccggtataa ggccggacgc
      901 agtgtttact gaccttgacg gcgcggcaga gtcttttctt gagatgaatc gcgagggaac
      961 gattatggtc atccatgccc atggggataa tatccctcag ctgcagtact gggttcccct
     1021 tttccccggg ccggttgtcg gtaccaccca gtcgcagcca gtccctcctc ttcataattt
     1081 tggcggtttc tctgatgggg accgtgcagc attcgcagcc gatgcactcg gagcagaacg
     1141 aattgttttt gtgggatttg atctcgacga tatgaacgtg ggtcctatga agagaggaaa
     1201 actgctgtgg gcccggcgtc ttctccggtt gatcggatat gaatgctgat gcattcatca
     1261 tcgatggcta cgttgatgaa cccgcgtgtc ttggggtgcc cccgtatatc tccccttaca
     1321 tacgaacgat agccggggtc tttacagcac atggaatcga gccgcgctac attaccatcg
     1381 accaggtcag ggcgaatcca cgcattctta ctcccgtccc cggcggcatt gcaatcatga
     1441 tagccggtgt gacggtcccc ggcaaatacc tgggaggaac tcccgcgact ctcaccgaga
     1501 tccagcaggt cgggacacag ctcaggggaa tgaaccggct catcacaggc cccgtcattt
     1561 ttggctattc gcgaggagga ggcgagcggg cagtccggaa gacaatctcg gggttcgatc
     1621 acctccttac cggttcacct gccgaatcgc ttgacaactg gctttctggt ggaacaacac
     1681 ctggtgatca ctcctatcaa cggtccgacc tctgggcggt aaccgggagt agcattatcc
     1741 gtcagcaccc ttccttcccg cacatcatgt gcgaggtcga gacagcccgc ggatgtacac
     1801 gtcaggtgac tggcggttgt tccttctgca ccgagccatt ctacggccat ccccgttatc
     1861 ggacgatcga ggggattcat catgaaatat cagcacttta tgctgccgga gcccggcatt
     1921 tccgcctggg aagacaaccg gaccttctca cctatggtgc cggccaaggg gaatttccgc
     1981 ggccacggcc ggaacttatc ggtgaactgt ttcagactat ccggtcagcc gcccccgggc
     2041 tgcaaacgct tcatatcgac aatatcaatc caggtaccct tgcacggcat ccggaagcag
     2101 gacgcaaggc gctcgaggag attgtccggt accacactcc gggggatgtt gcagcattcg
     2161 gcatggagac ggctgatccg atcgtggtca ggacgaacaa tctcaaagca gatcctgatg
     2221 aggtgatgga agcgatccgg attgtaaacg aagtcggagg agatcggaag gagggcatac
     2281 ccgatctgct tcccggcctg aattttattg ccggacttgc cggagagaca aaaagaacct
     2341 ttcttttgaa tgaacagttc ctcagccgcg tcctcgcatc ggggcttctg gtcagacggg
     2401 taaatatacg ccagctgatg ccgttcgaag ggacacgggc ttatgacgat aacactcttg
     2461 ggaagcatac ctcccagttc agggcattca aagagtatgt caggaaaaaa tttgatctgc
     2521 caatgctgag gcaggtgtac cccattggca cgatcctgca taacgttatc atcgaggaag
     2581 agggagctct ctctttcggg cggcagatgg gatcctatcc catccttatg ggaatacccg
     2641 ttcatcttaa gaggcatacg gttcttgata cagtggtcgt ggaccacggg atgcgatccc
     2701 taactggttt accggtaccc gttcaggtaa atctcctccc cagcccagcc ctgaaa
//
LOCUS       DAOOCO010000219         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_36799_length_2749_cov_3.630661, whole genome shotgun sequence.
ACCESSION   DAOOCO010000219 DAOOCO010000000
VERSION     DAOOCO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36799_length_2749_cov_3.630661"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK154_09790"
     CDS             <1..264
                     /locus_tag="PK154_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HPW11390.1"
                     /translation="HLLRDYGGVLTYAYSEDVEEFLDAVIKQQNFVIGRERNLMEKSL
                     MGALIAPRYLPFLIPSIAIHYEQNVPRIKKFFLKKTGRWKNEP"
     gene            704..1249
                     /locus_tag="PK154_09795"
     CDS             704..1249
                     /locus_tag="PK154_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11391.1"
                     /translation="MMSRLCTLFLVAFLVFLCGCATPEVRPGVSPEELGTLQDQVREF
                     MENPGYTIDVNNITVHEGVLQITNHNTSFLINPVDYSVLRAEFSGQDAVHSIANTSLH
                     TRAVDGIQTFFQNDSFDPVITKAVFDDDRYEIEGPGMFFRVNATTGTVVSFRLIGDDS
                     VQALNGSEQYAGAREALNSTL"
     gene            complement(1577..>2749)
                     /locus_tag="PK154_09800"
     CDS             complement(1577..>2749)
                     /locus_tag="PK154_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW11392.1"
                     /translation="FRVPRSTCQLFGTIKALSTIKNAIILVHGPKGCVYHINYILGLR
                     GDRPSEIFSTCLDEHDVIFGAEEKLTRAITELDGTFHPDFLFVLSCCASGIIGEDVNA
                     AVSGASIRSRAIAIESGGFEGDHAGGYKHTLARIASTLAEKTGESLPSTINLIGVLRA
                     GPDLQEIREILARSGISVNAVLTAGLRKDQLLRIGDVSLNVVVCEASGRDAAQELFEK
                     FGTPYVLCDLPVGGRATETFLDKIRTALLLPHNEENQREDTDLPLPHSSLRIAIFSGP
                     TRAIALSRFLGDLSCLPSLIVLDFPPPSFDQIKEAAGKDCLILIEPDHQAIQEALVGQ
                     KIDLILGGMLERPLASLLGIRVIDVMHGSQRTVGFEGARILSGLISGMGTPDARSP"
BASE COUNT          722 a          648 c          699 g          680 t
ORIGIN      
        1 catctcctga gagactacgg tggtgtgctc acgtatgctt actctgaaga tgtggaagag
       61 ttccttgatg ccgtgataaa acaacaaaat tttgtcatcg ggagggagag aaacctgatg
      121 gagaagagcc tgatgggtgc cctgattgcc ccacgatacc tcccttttct catcccttcg
      181 attgccattc actatgagca gaatgtccca cggatcaaaa aatttttttt gaaaaagacg
      241 gggcgatgga agaatgagcc atgaggaact ttcgcaggaa gaatcgtgag ggagtgccgc
      301 aacatcttca tcccggcttg gatcaactct tttgcgacag gggggagtga tcagaaaaaa
      361 gcctgtcccg ctttgatatg ccaccataaa tctcatggag taattacagg ggtatgccgg
      421 ttttgattca caccagggga tttacgggta ttacaaggcc cggtattttc cagataatac
      481 agaactgatt ggcgggagga tgatgcagca gatgtaattt tttatccaat cttcccggaa
      541 ccatagtttg ttttcagcct gatgagactt acgccgggga gatttgcacc tctcaatttc
      601 agagaatcat gccagggcat caaggaaggt caggcacatt tttcctcata acagctacag
      661 atcctctaaa tcattgtaaa gccaatgaaa gagagagatt actatgatgt ccagactctg
      721 caccctcttc cttgtcgcat tcctggtgtt tctctgcggt tgtgcgacac cggaggtcag
      781 gccgggcgtt tcaccggaag aacttggaac gctccaggat caggttcgcg aattcatgga
      841 aaatcccggt tataccattg atgtgaacaa tattaccgta catgaggggg ttcttcaaat
      901 aacaaatcac aatacttcgt tcctcatcaa tcctgtagac tactcagtat tacgggctga
      961 attttcagga caggatgcgg tacattcgat agccaatacc agcctccata cccgggcggt
     1021 cgatgggatc cagacgttct tccagaatga ttcattcgat ccagtcataa cgaaagccgt
     1081 atttgatgat gaccgatatg agatcgaagg cccgggtatg ttcttcaggg tgaacgcaac
     1141 aaccggaact gttgtctctt tccggcttat cggggatgat tcagtgcagg cactgaatgg
     1201 gagtgaacaa tatgctgggg caagggaagc attgaacagc acattgtaaa gtgggttaac
     1261 ggcattcaac tccttttacc aataattaaa aggagtatat cgcgtgatag acaatcccct
     1321 ggagtcaagc ctttggctta tacccgcctc tgatatgtcc ccgcgatgca acagaccgga
     1381 ttggagaata caaatctcct tgtagaacat attgaaaggt atgtcacctt ttttcgtgga
     1441 tttgggaact cagactcctg tattttccga caggattgat cagaaaaggg gagtaaaacc
     1501 tttataccta tttttttggt ttttagaaga aaaatgataa atggggacct cttttttccg
     1561 atatggacat gatcagttat ggagaacggg catctggtgt gcccattccg gatatcagtc
     1621 ccgagagaat ccgggcgcct tcgaacccga cggtccgctg actgccgtgc atcacgtcga
     1681 taacccggat accaaggagg gaggcgagag gacgttccag catcccgccc aggatcaggt
     1741 caatcttctg tccaaccagt gcttcttgaa ttgcctgatg atctggttca atgagaatca
     1801 ggcaatcctt tcctgcagcc tcttttatct gatcaaatga ggggggcggg aagtccagaa
     1861 cgatgaggga aggcaggcat gagagatcgc cgagaaaccg ggagagagca atggcacggg
     1921 ttggccctga aaagatggcg atacggagtg aggaatgagg gagaggaagg tccgtatcct
     1981 ctctctgatt ttcctcattg tgagggagca aaagcgctgt ccggatctta tccagaaagg
     2041 tttccgtagc tcttcccccg acaggcaggt cacacagtac gtagggggtg ccaaattttt
     2101 cgaagagttc ctgggcggca tccctccccg atgcttcgca gaccacaaca ttcagggata
     2161 catccccgat gcgaaggagc tgatccttcc ggagccctgc agtgagtacg gcattgactg
     2221 agatccccga tctggcaagt atctcccgta tttcctggag atcaggcccg gcccggagca
     2281 ccccgatcag attgatcgtt gaggggagtg attccccggt tttttctgca agggttgatg
     2341 cgatacgggc aagggtatgc ttatagcctc ctgcgtgatc cccttcaaag ccacctgatt
     2401 caatggcaat cgcccgtgac ctgatggatg caccacttac ggcagcattc acatcctcgc
     2461 caatgattcc tgatgcacag caggagagaa caaaaagaaa gtcgggatga aatgtcccgt
     2521 caagctcggt tattgccctg gtgagtttct cctcagcgcc aaaaatcaca tcgtgctcat
     2581 cgagacacgt tgaaaatatc tcgcttggcc ggtcacccct aagaccaaga atataattaa
     2641 tgtggtaaac gcatcccttc ggaccatgaa caaggataat ggcatttttt atcgtcgaaa
     2701 gcgctttgat ggtaccgaac agctgacagg tactcctcgg aacccggaa
//
LOCUS       DAOOCO010000220         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_37116_length_2734_cov_4.296006, whole genome shotgun sequence.
ACCESSION   DAOOCO010000220 DAOOCO010000000
VERSION     DAOOCO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37116_length_2734_cov_4.296006"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1640)
                     /locus_tag="PK154_09805"
     CDS             complement(177..1640)
                     /locus_tag="PK154_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW11393.1"
                     /translation="MTHDVEETLDPDDWKAMRALGHRMLDDALDYIGTLRDRPVWQHA
                     PPEVKAHFDGMPPDGPEHPDEIYQEYREYILPYQLGNSHPRFWGWIAGSGTVMGMYAE
                     MLASSMDAVSGAFSFMSNNYVESQVIGWCKAMLGYPMTASGLLTSGCSASNLIALAAA
                     RNTKAGFDLRKEGMQNAKKKMTLYCSDEAHSSLQKAVELLGFGNDALRRIPANDHMQI
                     DLAALESRIKEDRADGFYPVCIIGAAGTTNTGAVDDLHALADISTREGCWFHVDGAFG
                     AWAAIAPESRHLVAGMERADSLAFDLHKWMYLQYPIGCVLIRDAELHRRAFSLTPTYL
                     AHGEGDRGLTGVDLPWLDDYGFELSRGFHALKAWMTIKEQGTEKYGRLIQQNIEQAQY
                     LARLVEAAPELELALPVSLNIVNFRYAKPGMNDERLDHLNKQIELDLQEAGIAVPSTV
                     TIRGRKYLHIAITNHRSRKEDFDLLVREVIRAGKSKS"
     gene            complement(1844..2338)
                     /locus_tag="PK154_09810"
     CDS             complement(1844..2338)
                     /locus_tag="PK154_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW11394.1"
                     /translation="MAKIKQKIVPCIWLEKDAEKAAEYYISVFRNSKILETAYYPKAS
                     EAVSGQKAGSVMTVKFEIEGQEFMVLNGGPAFRPNEAISFMVYCDTQEEIDYYWERLS
                     FVPEAEICGWLKDRFGVTWQIVPTVLEAMMTDKDPEKVERVTAAFLKMKKFDIGELKK
                     AYNG"
     gene            complement(2532..2705)
                     /locus_tag="PK154_09815"
     CDS             complement(2532..2705)
                     /locus_tag="PK154_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW11395.1"
                     /translation="MTLPRWQCLECGYLCDPERGDPKGGIAPGVPFDDLPKTWRCPEC
                     GVSRLKKGAFVRR"
BASE COUNT          572 a          806 c          718 g          638 t
ORIGIN      
        1 atcgaccttc tttttcacgc tccccgcact gccatcgtaa ggatcaggaa agagatggtt
       61 ccagacatct gttcccggcc atcgtattca tgaccggaca gggtggatcg atcagggacg
      121 agatatgacc gaagacttct gcagggaatt tcacttgaca ggcagagggg tgatcctcat
      181 gatttgcttt taccggcccg gatcacttcc ctgacgagga gatcgaagtc ttctttcctg
      241 ctgcgatggt tggtgatggc gatatgcagg tacttccttc cccggatggt tactgttgag
      301 ggaaccgcta tccccgcttc ctggaggtcg agctcgatct gcttgttcag atgatcgagt
      361 ctctcgtcgt tcatacccgg ctttgcgtac cggaagttga cgatgttcag ggagaccgga
      421 agcgccagct cgagttccgg ggcagcttca accagccggg cgagatactg cgcctgttcg
      481 atattctgct ggataaggcg gccatacttc tccgttccct gctcttttat ggtcatccag
      541 gccttgaggg cgtggaagcc gcgggagagc tcgaatccgt aatcgtcaag ccaggggagg
      601 tcgacaccgg tcaatcccct gtcaccctcg ccgtgggcaa ggtaggtcgg ggtcagggag
      661 aatgccctcc ggtgcaattc agcgtccctg atgagcacac agccgatagg atactgcagg
      721 tacatccact tgtgcaggtc aaaggcgagt gaatccgccc gttccatccc cgcaacgagg
      781 tgccgggatt caggggcgat ggctgcccag gcaccgaatg ccccatcgac atggaaccag
      841 cacccctctc tcgtgctgat atcggcaaga gcatgcaggt cgtcaaccgc cccggtattc
      901 gtcgtcccgg cagccccgat gatacagacc gggtaaaacc cgtctgccct gtcctccttt
      961 atccttgatt caagggctgc aaggtcgatc tgcatgtgat cgtttgcagg gatccttctc
     1021 agcgcatcgt tcccgaatcc cagcagttca accgccttct gcagggagga atgggcctca
     1081 tcggaacagt agagggtcat cttctttttg gcgttctgca tcccctcctt ccgcaggtca
     1141 aacccggcct tcgtgtttcg ggcagcggcg agtgctatca ggttcgaggc agagcacccg
     1201 ctcgtgagga gcccgcttgc cgtcatggga tacccgagca tcgccttgca ccagccgatg
     1261 acctgggatt cgacgtagtt gttgctcatg aaggagaatg ccccgctgac ggcatccatc
     1321 gaggacgcaa gcatctcggc atacatcccc atcacggtcc cggaaccagc gatccacccc
     1381 cagaacctcg ggtggctgtt tccaagctga tatggcagga tatactcccg gtattcctgg
     1441 tatatttcat ccggatgttc cgggccatca gggggcatgc cgtcgaaatg ggctttcacc
     1501 tcgggggggg cgtgctgcca gacgggccga tcccgcaggg tcccgatata gtccagtgca
     1561 tcatcgagca tccggtggcc aagtgcccgc atcgccttcc agtcatccgg gtcaagggtc
     1621 tcttcaacat cgtgggtcat ggtactgcct cttttctggt gcgattatct cccctgaccc
     1681 ctataggaaa aatcagggaa tatcttttgt gattcggaga tgactcagag aaccggtctc
     1741 ctgcaggtaa aatgaagaga ggggccgaac ttccgccccg gatacccctg ccagaacatc
     1801 aatcagccga agatcctgaa ggaaaggcct gaggtagcca tggttaccca ttataggcct
     1861 tctttaactc cccgatatca aacttcttca tcttcaggaa cgcggcggtc acccgttcca
     1921 ccttctccgg gtccttatcg gtcatcatcg cctcgaggac cgttgggacg atctgccagg
     1981 tcaccccgaa cctgtccttc agccacccgc agatctctgc ttccgggacg aatgacaaac
     2041 gctcccagta ataatcgatc tcttcctgcg tatcgcagta caccatgaac gagatcgcct
     2101 cgttcggcct gaatgccggc ccgccgttca gcaccatgaa ctcctggcct tcgatctcga
     2161 atttgacggt cataaccgat cccgccttct ggccggagac cgcttctgaa gcctttgggt
     2221 aataagcggt ctccaggatc ttcgaattcc tgaagacaga gatgtaatat tctgccgcct
     2281 tctctgcatc tttttcgagc cagatacaag gaacaatctt ctgttttatt ttcgccatcg
     2341 catttcacct ctttcatccc tgttcaaaaa ataaccggga ttcccgtgtt actctcctga
     2401 aagggaggat tgctcgtgag tagatgatca agactgatat gctttttctg cattggcaaa
     2461 atctgcaaat gaagcacaag gtaaaaaagt ctgaaaaagc tcagaaaaat tccccatttc
     2521 ttccgggccg gttatcgccg aacaaatgcc cctttcttca gcctcgatac accgcattcc
     2581 gggcaccgcc aggtttttgg caggtcatcg aaggggaccc ccggtgcgat cccccccttc
     2641 gggtctcccc tctcgggatc acagagatag ccgcattcga ggcactgcca acggggaagg
     2701 gtcatggatg tcggttcgct tcaaagggga taac
//
LOCUS       DAOOCO010000221         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39086_length_2644_cov_2.764774, whole genome shotgun sequence.
ACCESSION   DAOOCO010000221 DAOOCO010000000
VERSION     DAOOCO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39086_length_2644_cov_2.764774"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK154_09820"
     CDS             <1..366
                     /locus_tag="PK154_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11396.1"
                     /translation="VITDLTPEQVRDGIKVRYEVDPYEVAGRLRASYDEALTVPYSAG
                     VYSSADVAKVVAGRLIDAGLPVLVERLPEAPPNVQVTETAEGRPVHGHQGPVWVIRTG
                     YRAPDTSARTREKKYQDNH"
     gene            637..1629
                     /locus_tag="PK154_09825"
     CDS             637..1629
                     /locus_tag="PK154_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPW11397.1"
                     /translation="MSTQSGSKIAVFAAIGGNLIIAIIKFIAAALSGSSAMIAEGIHS
                     LVDTGNGLLVLLGMKRARQPADESHPFGYGKSLYFWTNIVAISIFGIGGGMSLYEGIS
                     HIRHVAPGGEMGDPTAAYIVLGLSLLVEGASFTVAIRQFLKAKGDIGVWQFIKNSKDP
                     SLYTIVLEDSAAMLGLIFAFIGIFLGHLLNNPYLDGIASIAIGLLLMSVAWILASRTS
                     GLLLGEGVNPGELADIRRRVESDSAVERAGDILTMYMGPHDLLVNMGVCFIPGTTAEQ
                     MHEAIRRIEADLRNTYPATNRVYIEAESLPVVATGESRQNLGECAGVPGEHPGK"
     gene            2018..2404
                     /locus_tag="PK154_09830"
     CDS             2018..2404
                     /locus_tag="PK154_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW11398.1"
                     /translation="MTSPGPQVRTIDAYIGTFSPKVQKILEQLRSAIRETAPEAAETI
                     KYGIPTFTLNGNLVHFAAFAHHIGFYPTPSGIEAFKKELSPYTTSKGTVQFPIDDPIP
                     FDLVRRMVRFRVKENMEEGIKKRNIR"
BASE COUNT          619 a          692 c          726 g          607 t
ORIGIN      
        1 gtgataaccg atttgacccc ggaacaggtc agggacggta tcaaagtccg gtacgaagtc
       61 gatccctatg aggttgcggg aaggttacgg gcatcgtacg atgaggcttt gacggtcccc
      121 tacagtgccg gggtgtatag ttcagctgat gtggcaaagg tcgtagccgg caggctgatt
      181 gacgcagggc tccctgtcct ggtggagcga ttgccggaag ctccccccaa tgtgcaggtg
      241 acagagactg cagagggcag gccggtgcat ggtcaccagg gccccgtgtg ggtgatcagg
      301 acaggttata gggcgcccga tacttctgca aggacccgtg aaaaaaaata ccaggataac
      361 cattgaaaga gcagtgtctt cgctgtattc cctctcagca accttttttt tcctccatga
      421 acaatgggtt ttagcttccc ggacaagggt tcagagatcc cactatttca ttgttgaggt
      481 gtattctctc cgggctcatc acgaataaga taaatgcccg gtaacccaac actggtagaa
      541 cagcccgttt ggaaagaagg taccctgcca gttgcacccg gagatttatc cttgggattg
      601 ggatgaatgg ggccataaaa gggtgagagg gagcaagtgt cgacacagag cggttcaaaa
      661 atagcagtct tcgcggccat cggtggcaac ctgatcatcg cgatcatcaa gttcattgcg
      721 gcagcactct ccggttcatc ggccatgatt gccgagggta tccactcgct cgtcgatact
      781 ggaaacgggt tgctcgtgct gctgggaatg aaacgcgcca ggcagcccgc agatgagagc
      841 cacccgttcg gctatggcaa gtcgctctac ttctggacga acatcgtagc gatctcgatc
      901 tttggcattg gtggcggtat gtctctttac gagggcatct cccacattcg ccatgtcgca
      961 ccgggtggcg agatgggcga cccaaccgcg gcgtacatcg tgctcggtct ctccctcctg
     1021 gtcgagggtg catcattcac tgtcgccatc aggcagttcc tgaaagccaa gggagatatc
     1081 ggggtctggc agttcatcaa gaactcaaag gacccgagcc tctatacgat agtgcttgag
     1141 gatagcgcag ccatgctcgg cctcatcttc gcattcatcg ggatattcct gggtcatctg
     1201 ctcaacaatc cctatctcga tgggattgcg tcgatcgcaa taggcctgct gctgatgagt
     1261 gtggcatgga ttctcgcgtc gcggacgagc gggttgttgc ttggggaagg ggtgaatccg
     1321 ggcgaactcg cagacatccg gcgtcgcgtg gaatctgatt cggcagttga gcgtgccggg
     1381 gatatcctca ccatgtacat gggtcctcac gatctgctgg tgaacatggg agtctgcttc
     1441 attccaggga ccacggcaga acagatgcac gaagcgatcc gtcgcatcga agccgacctg
     1501 cgcaatacat atccggcaac gaaccgtgtc tatatcgaag ccgagtcgct gccggtcgta
     1561 gcgaccggcg aaagtcggca gaatttgggg gaatgtgcag gcgtccccgg agaacatccc
     1621 ggcaagtagt aaatcggggt gatgttatgg agcggggggt gatcgccccg tgatttcctg
     1681 ggattttgta ccgggaaccc tgcctgcatt tgcgaagagc ctctgcacgt tccttcaatg
     1741 gattctgccc cgcgtctgtt tgatccctgc agtatccatc tcggatcaat ccacttctgg
     1801 ttcacttatt atgcggtgaa catacctgtg ggtactcctg gatcgacacg gtcacctcac
     1861 tcttgcagaa actgatgtgg ttctccacga cctgtcaatg caggacggtt gaaggcagta
     1921 ttctctcgcc cgtcaagtaa atgcgggtga tcctgaggat aaggtcggtt cttttgcgga
     1981 actatcatca cccaacacga tgatgaagaa ttaccgcatg acttcaccag ggccacaggt
     2041 caggaccatc gatgcgtata tcgggacctt ttctccaaaa gtccagaaaa ttctggaaca
     2101 gctgaggtcg gcaatccgcg agactgctcc ggaagcagca gaaacgatca aatacgggat
     2161 acccaccttc accctcaacg gcaacctggt gcatttcgct gcatttgcgc atcatatcgg
     2221 attctatccg actccatcag gaatcgaggc gttcaaaaaa gagttgtccc cctatacaac
     2281 ctcaaagggt accgtccagt ttccaatcga tgatccaatc ccgtttgacc tggtgagaag
     2341 gatggtccga ttcagggtaa aggaaaacat ggaagaagga ataaaaaaga gaaatatccg
     2401 gtaacccctg gaatctatgg accacttccc cctgccacca tttgacgata tagggtgagc
     2461 ctggcctgga atcatcagaa aaaatcctgc aacgtctgaa attttcatcc aaggaatttt
     2521 ggaatcatca tacaggttgc cgcgtagatt ttatcctaga ttccgttctg ttcccctcag
     2581 cgcccgctgt ctgtgtagca ggaactgata accgcatctc tgacgcaaca cggcgatacc
     2641 acct
//
LOCUS       DAOOCO010000222         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39162_length_2640_cov_4.505996, whole genome shotgun sequence.
ACCESSION   DAOOCO010000222 DAOOCO010000000
VERSION     DAOOCO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39162_length_2640_cov_4.505996"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PK154_09835"
     CDS             complement(<1..1157)
                     /locus_tag="PK154_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HPW11399.1"
                     /translation="MRALMISGDRSGSGKTSISLAIAALLSQKMTVQTFKVGMDYIDP
                     SYLTGVTGRPCRNLDTFVMERTDLARIFAHGMEGSDCGLVEGVRGLYEGAESLGDEGS
                     SAAVAKLLNIPVVLVVSARSITRSAAAIVKGFQSFDPAIDIRGIILNNVSGPQHVRKA
                     TEAIEHHCGVPVIGAVPRQPDMELAMRHLGLVPYLEGKTAPAFLRRIQDITAMVGDHI
                     DPDLLLGLSATVPTPPGHDPLFEPAEAPDTTIAVALDEAFNFYYADLFDHLRAGGAKV
                     VTFSPIHDRLPEADGYIFGGGYPELFLHELEANDRMRDAVHERSREGIPIYAECGGLM
                     YLTGAIRTKAGWQGSDKEESCAMCGVFDGETMMPARRIVSYVEGESQGGNPL"
     gene            complement(1145..2218)
                     /gene="cfbD"
                     /locus_tag="PK154_09840"
     CDS             complement(1145..2218)
                     /gene="cfbD"
                     /locus_tag="PK154_09840"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HPW11400.1"
                     /translation="MKYMQPRPSSIVAALYTARDLEVDVAVLHGPSGCSFKHARLLEE
                     DGMRVLTTSLADHEFIFGGQEPLERVLRYAEEQFHPHRMAVVGTCVSMIIGEDLKSAV
                     TLSGVSTPTIAIEIHAGYPENIEGVIATLEPAAEAGWISADELERQKYLLGMANEVER
                     LRGAACKPYIEPSRGDLKHLVAGRLAALAGSGKRGVAIMNAKKETAYMFADELLALHD
                     ICPCADITYLANLEARGLPKVRQDARRILAGMTGRGLEPGLLGALDEYGANGEAAGKR
                     LSELDPEFALIVGVPHAIPREYTDGIECFSITNGPRQVEPLRDLGHRHVVVEVDLHPR
                     TLGVKDIVESEFGAILRSMSCEP"
     gene            complement(2227..>2640)
                     /locus_tag="PK154_09845"
     CDS             complement(2227..>2640)
                     /locus_tag="PK154_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HPW11401.1"
                     /translation="DPSRLATFQAIPGRMSQTSEGGIRIIDNSNSGTSALTTRSAVQY
                     ARKITGREEITLVTGKETGAVCEGFPREDILALIGEIQPARVIMVGSEYAGIGDDPGC
                     RGIPFHYAGSLAEGISIASTLTSCGSIVLSVKCWR"
BASE COUNT          513 a          836 c          730 g          561 t
ORIGIN      
        1 agcggatttc ctccctgact ctctccttcg acataactga caatccggcg tgcaggcatc
       61 atcgtttcac catcaaagac cccgcacatc gcgcaggact cctccttgtc tgatccctgc
      121 catcccgctt tcgtccgtat cgccccggtg aggtacatca ggcccccgca ttcggcgtat
      181 atcgggatgc cctcccgcga cctctcatgt accgcatccc tcatccgatc gtttgcctcg
      241 agttcatgca ggaacagttc ggggtatccc cctccgaaga tgtacccgtc cgcctccggt
      301 agccggtcat ggatcgggct gaaggtgact acctttgccc cgccagccct gaggtggtca
      361 aagagatccg cataatagaa gttaaacgct tcatcgagtg caactgcgat ggtcgtatcg
      421 ggtgcctcgg caggctcgaa caggggatca tggccgggag gggtggggac cgtggctgac
      481 agtccgagga ggagatcggg atcgatatga tcccccacca ttgcagtaat gtcctggatg
      541 cgccggagaa acgcaggagc agtctttcct tcgagatagg gcaccagccc caggtggcgc
      601 atcgcaagct ccatgtcagg ctggcgtggt actgccccga tgaccgggac cccgcaatgg
      661 tgctcgatcg cctcggtagc cttccggaca tgctgtggcc cggagacatt gttcaggatg
      721 atccccctga tatcgatcgc cgggtcaaat gattggaatc ccttcacaat cgcagccgca
      781 ctccgggtga tgctccgtgc acttaccacc agcacgaccg gtatgttcag gagctttgcg
      841 accgctgcac tgctcccctc atcgccgagg gactctgccc cctcgtaaag acccctgaca
      901 ccttctacaa ggccacaatc ggatccctcc ataccgtgag cgaagatcct ggcaaggtct
      961 gttctttcca tcacgaaggt atccaggtta cggcagggac gccctgtaac cccggtgagg
     1021 taggagggat cgatgtagtc catgcccact ttgaacgtct ggaccgtcat cttctgcgag
     1081 agcagcgcag cgatcgcgag gctgatgctg gtcttcccgc tccctgaacg gtcccctgag
     1141 atcatcaggg ctcgcatgac atgctccgga ggatcgcacc aaactcgctc tcgacaatat
     1201 ctttcacgcc aagggtccgg ggatggagat ccacctcaac gacaacgtgc cggtgcccaa
     1261 ggtcacggag tggctcgacc tgccgcgggc cgttcgttat cgagaagcat tcgatcccat
     1321 cggtatattc ccgggggatc gcatgcggaa ccccgacaat gagcgcaaac tcagggtcga
     1381 gttctgaaag ccgcttccct gccgcctctc cgtttgcacc gtactcatcg agggccccta
     1441 aaagcccggg ttccagcccg cgcccggtca tcccggcaag gatccgccgg gcatcttgcc
     1501 ggaccttcgg aagacccctg gcttcaaggt ttgcaagata cgtgatgtcg gcacaggggc
     1561 agatgtcatg gagcgccagg agttcatccg cgaacatgta tgcggtctct ttcttggcgt
     1621 tcatgatcgc aacccccctc tttcccgaac cggcaagggc ggccagcctt cccgcaacaa
     1681 gatgtttgag atccccccgg gagggctcta tatacggttt gcaggctgct ccccggagac
     1741 gctccacttc gtttgccatc ccgagcaggt atttctggcg ttcgagttca tcggccgaga
     1801 tccatcctgc ctcagcggcg ggttcaaggg tggcgatcac tccttctatg ttctcggggt
     1861 atcctgcatg gatctcgatc gcgatggtcg gcgttgagac gcccgagagg gttactgctg
     1921 acttgagatc ctcaccgatg atcatggaga cacaggtgcc cactaccgcc atccggtgag
     1981 ggtggaactg ctcctcggca tacctgagga cccgttcgag cggctcctgc cccccgaaga
     2041 tgaactcgtg gtcagcgagc gaggtggtca gcacacgcat cccgtcctct tcaaggagcc
     2101 gagcatgctt gaacgagcat cctgacggcc cgtggagtac ggctacatcg acctcgagat
     2161 cgcgggctgt atagagggca gcgacaatcg aactcggcct cggctgcata tacttcatgg
     2221 cttctctcac ctccagcact tcacagaaag gacaatgctt ccacagctgg taagggtact
     2281 tgcgatggaa attccctcgg caagggatcc agcatagtgg aacgggatac ccctgcatcc
     2341 gggatcatct ccgatcccgg catattcgct gcctaccatg atcaccctgg caggttggat
     2401 ctcaccgatc aaagccagga tatcctctct tggaaatccc tcgcagaccg ccccggtctc
     2461 tttcccggtg acgagcgtga tctcctctct gccggtaatc ttccttgcgt actgcactgc
     2521 agaccgggtg gtcagtgcac tggtccctga attggaattg tcaatgatcc tgatccctcc
     2581 ttcggatgtc tgggacatcc tgcccgggat ggcctgaaac gttgcgagcc gtgacgggtc
//
LOCUS       DAOOCO010000223         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39262_length_2636_cov_3.390934, whole genome shotgun sequence.
ACCESSION   DAOOCO010000223 DAOOCO010000000
VERSION     DAOOCO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39262_length_2636_cov_3.390934"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /locus_tag="PK154_09850"
     CDS             <1..2028
                     /locus_tag="PK154_09850"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPW11402.1"
                     /translation="YFFHHRDHLVAGSVRAPRIDLGNEDLVRAHIHAVWLSYVRIPLG
                     SSIDEIVDLGIKGYPLQTNVKEQIHLSDERLKKCFNDVKEVLSRCGHDLAENSWYNDD
                     WLMTTLRSAPKDFDGAFQRFRELFKSADQLWEEANNQMRIPSRDKDKMERAKNQRMEA
                     ERQKNLLCNHTATKEESDFYPYRYLGSEGFLPGYNFPRLPIRAFIPSSSNEGEFISRP
                     RFLAVTEFGPQAFLYHEGAKYQVKRLTPPPGGLQSLRHVAKICTNCGYFHTDVKCDLC
                     ENCGVIFDATSSEVVPLLEMTNVTTQRRQKITSDEEERKRFGYHLTSHFRFAPGTDGK
                     KRTLEGIVQDSSATPILNLVYAPAASLFRINHGWRIQREKSFHIDMATGDWMRTPDSE
                     ETDAEPSTAIQPEAVKLFVQDTMNLLLVNFIGDSSIDDGFQASLQYALQRGMEKVFQI
                     DESEIASERIGTGTNTAILFWEASEGGVGVLKRLVTEPETLRQVATAALERCHFDPDN
                     LDDVAVKECSHACYECLLSYSNQRDYRKLDRHLLPNFLARLKNGTTLQQKEDRTYDEQ
                     YQWLRAQTDSQSNLERQFLDHLYQSQRRLPDGAQERLADYYSEPDFVYDPSTLIFCDG
                     SVHDFPDQMRVDEDSRTGLKEKGYRIVVIRYDKDLEEQIRAYPDVFGKGRG"
     gene            2031..>2636
                     /locus_tag="PK154_09855"
     CDS             2031..>2636
                     /locus_tag="PK154_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPW11403.1"
                     /translation="MDESGIGSIVKCRNREWVIVPSQDEHITLLRPLTGSEKELCGIF
                     NPLREKGLDTIEPATFPLPNPDHSGDAIGVGLLWNAARLSLRDGAAPFRSLGRVSVRP
                     RPYQFVPLLMALRIQPVRLLIADDVGIGKTIESLLIAREMLDRGEISRLCVICPPYLC
                     DQWQKELTEKFHIDAVVVRSGTIGRLERELPSGDWSIFNYYP"
BASE COUNT          631 a          661 c          704 g          640 t
ORIGIN      
        1 tacttcttcc accaccgaga tcatctggtc gcaggatctg tccgtgcacc tcggattgat
       61 cttggcaatg aagaccttgt tcgtgcacac atccatgcag tctggctctc ctatgtccgg
      121 atcccgctcg ggtcgtcaat agatgagatt gtcgatctcg ggatcaaagg gtatccgctc
      181 cagacgaatg tgaaagaaca gatccacctc tccgatgaac gattaaaaaa atgttttaat
      241 gacgtgaaag aggtcctttc ccgttgcggt catgatcttg cagaaaattc ctggtacaat
      301 gatgattggt tgatgacgac cctacggagt gcgccgaaag attttgatgg ggcattccag
      361 cgcttccggg aattattcaa gtcggcggac cagctatggg aagaggcgaa caaccagatg
      421 cggattccca gccgtgataa ggacaagatg gaacgggcaa agaatcagcg gatggaagcc
      481 gaacggcaga aaaatttgct ctgtaatcat acagcaacga aagaggagtc agatttctat
      541 ccataccggt atctcgggag cgaaggattt ttacccggtt acaactttcc ccggctcccg
      601 attcgtgcat ttattccctc cagttccaac gagggcgaat tcatttcccg gccccggttc
      661 ctagcggtta ccgagttcgg gccacaggct ttcctttatc acgagggcgc gaaataccag
      721 gtgaaacggc tcactccccc tccgggtggg ctccaatcac tccgccatgt agcgaagatc
      781 tgcacgaatt gtgggtattt ccataccgat gtaaaatgcg atctttgtga gaattgtggg
      841 gttatcttcg atgccacctc ttccgaagtc gttccgctgc ttgaaatgac gaatgtcaca
      901 acccagcgtc ggcagaaaat aacatcagac gaggaagaaa ggaaacgttt tggctaccac
      961 ctcacctccc atttccgatt tgcaccagga actgacggga agaaaaggac gctcgagggt
     1021 attgtgcagg atagttcagc tacacccata ctgaaccttg tgtatgctcc tgccgcatca
     1081 ctgttccgta tcaatcatgg gtggcgtatc cagcgtgaga aatcgtttca tatcgatatg
     1141 gctaccgggg actggatgcg gactccggat agtgaagaga ccgatgcaga gccgagtact
     1201 gcgatccaac ctgaagcggt gaaactgttt gtgcaggata ccatgaatct gttattggtg
     1261 aacttcatcg gcgatagttc gatcgatgat ggtttccagg cgtcgctcca gtatgcactt
     1321 cagagaggga tggaaaaggt gtttcagatt gatgaatctg agatcgcatc agaacggatc
     1381 ggcactggaa caaacacggc gatcctgttc tgggaagcat ctgaaggagg ggttggggtt
     1441 ctcaaacgat tagtgactga gcctgagaca ctccgacaag tcgccactgc cgcactggaa
     1501 cgttgccact ttgacccgga caacctggat gatgtagcgg taaaagagtg ctctcacgcc
     1561 tgttatgagt gcttactctc ctattccaac caacgggatt accggaagct cgaccggcac
     1621 ttattgccca acttccttgc ccggttgaag aatggtacta ccctccagca gaaggaggac
     1681 cggacctatg acgagcaata tcaatggctc cgtgcccaaa ctgattcgca gtcgaacctt
     1741 gaacggcagt tcctcgatca tctctaccag agccagcgcc gactgcctga tggggcacag
     1801 gaacgccttg ctgattatta tagcgaaccg gatttcgtgt acgatccaag caccctcatc
     1861 ttctgtgatg gttcggtgca tgatttcccc gatcagatga gagtcgatga agattcccga
     1921 acaggtctga aggagaaagg gtatcggatc gtcgttattc ggtatgacaa ggaccttgag
     1981 gaacagatcc gtgcgtatcc tgatgtcttc ggtaaaggga gagggtaagg atggatgagt
     2041 cgggcattgg ctcgattgtt aaatgccgga accgtgaatg ggtgattgtt ccttcccaag
     2101 atgaacacat caccctgctt cgcccgctga ccgggagtga gaaggagctc tgtgggattt
     2161 ttaatcctct tcgtgaaaag ggtctcgata caatcgaacc agcgacgttt ccgcttccga
     2221 atcccgatca ttccggggat gccatcggag taggattgct ctggaatgcg gcacggttat
     2281 cattgaggga tggtgccgcc ccgtttcgtt cccttggtcg tgtatcggtc cggcctcgtc
     2341 cttaccaatt tgtcccgttg ctcatggcgc tgcgtatcca gcccgtccgg ctcttgatcg
     2401 cggatgatgt cggtattggt aagaccatcg aatcattact catcgcccgc gagatgctcg
     2461 acagagggga gatatcccgg ctgtgtgtga tctgtcctcc ctatctctgc gatcagtggc
     2521 agaaggagct taccgaaaag ttccacatcg acgctgttgt ggttcgttcc ggtactattg
     2581 gacggctcga acgcgagtta ccatcaggtg actggagtat cttcaactat tacccg
//
LOCUS       DAOOCO010000224         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39543_length_2624_cov_5.380304, whole genome shotgun sequence.
ACCESSION   DAOOCO010000224 DAOOCO010000000
VERSION     DAOOCO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39543_length_2624_cov_5.380304"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK154_09860"
     CDS             <1..315
                     /locus_tag="PK154_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPW11404.1"
                     /translation="IGRTGRAGKEGRSFTFVSGKEMWKLRDIQRFAKIKIALQSVPKE
                     HEIISRKAELMARRVRDLIDTGRLDPEILRVQQIMGEEYTSLEVAAALLSLYVGSGKP
                     EK"
     gene            456..2129
                     /locus_tag="PK154_09865"
     CDS             456..2129
                     /locus_tag="PK154_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPW11405.1"
                     /translation="MISVLLVHDNSDLIEGTRASLEKSGEVRVDTVPTTKQALEILKS
                     RTYDVIVSYYHLPEVNGIEFFPEMNGVELLKYLKSHGNPTPFILYARMKDTLVLEDVN
                     NATEVKFSRGEDQKKPVTELRDLIYQVVMRKKMERDQALRTELLDSILTLSPLWICQI
                     RGGVIDWANRTMTAGLGHPEGGLVGKNASAFFASREAFDRASRELSLDVDQEGWGHAT
                     TQLKRCDGTLVHCSLMVHALDPKDPSRGQVLVCEDIAEKVRLTETVRESDLRVRELLL
                     HASSLIIKLDPDGTITFFNTFAQVFFGYSETEIMGKKIIGTLIPHRKGEGHQLTRFVS
                     DMGSNGDGFAIRITEMLLRDGQPAWVAWTNKALRDEDGHILEIVCIGHDITDHEHRDR
                     SRISTALWKDRVIADSDVTDDVFETVFNICLEIAKEGREGKKVGTAFVIGDTENVLSK
                     SRQLILNPFAGHRIEDRMVTNHDIRENIKELAQLDGAFVIRGDGLIEAAARYITVDTS
                     AVGIAKGLGTRHSSVAGITLVTKAIGIVVSQSGGKISIFRNGRMLQEIG"
     gene            2215..2385
                     /locus_tag="PK154_09870"
     CDS             2215..2385
                     /locus_tag="PK154_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HPW11406.1"
                     /translation="MNNQRKYGGPSSSGPRTMTKTVCSDCGKECEVPFTPTQGRPVYC
                     RDCLPKHRKPRY"
BASE COUNT          579 a          785 c          787 g          473 t
ORIGIN      
        1 atcgggcgga ccggccgggc cggcaaggag gggaggtcgt tcaccttcgt atccggcaaa
       61 gagatgtgga agctccgtga tatccagcgg tttgcaaaga ttaagatcgc cctccagtcg
      121 gtgccaaagg agcatgagat catatcccgg aaggccgagc tcatggcccg cagggtccgc
      181 gacctgatcg ataccggcag gcttgatccc gagatcctcc gggtgcagca gatcatgggg
      241 gaggagtata cctcgctcga ggttgctgca gccctcctct ccctgtatgt cgggagcgga
      301 aaacccgaaa aatgaacatt ttcgggcaca gcacgacaaa aggaccggga ttttgcgggc
      361 gattggtcat cccgattcaa taattattta ttcgataacc cacctactct atctagcctg
      421 cgccgtggtg aagaacggat ggtgaagaac agaggatgat ctccgtactc ctggtccatg
      481 acaactccga cctgatcgag ggcacccgtg cctcccttga gaagagcgga gaggtccggg
      541 tcgacaccgt cccgaccacc aaacaggcgc tcgagatcct caagagcagg acctacgatg
      601 tgatcgtctc ctactaccac ctcccggaag tgaacgggat cgagttcttc cccgagatga
      661 acggggtcga gctcctcaag tacctcaagt cccatggcaa cccgaccccg tttatcctct
      721 atgcacggat gaaagacacc ctggtgctcg aggatgtgaa caacgccacc gaggtgaagt
      781 tttcccgggg ggaggaccag aaaaagccgg tcaccgagct ccgcgatctc atctaccagg
      841 tggtgatgag gaagaagatg gagcgggacc aggccctccg gaccgagctc ctcgattcga
      901 tcctcaccct ctcccccctc tggatctgcc agatccgggg cggggtgatc gactgggcaa
      961 accggacgat gaccgccggg ctcggccacc cggagggcgg gcttgtcggg aagaacgcct
     1021 ccgccttttt tgccagccgg gaggcctttg accgggcatc ccgggaactc tccctcgatg
     1081 tcgaccagga gggatggggg catgcaacca cccagctgaa gcggtgtgat ggcaccctcg
     1141 tccattgcag cctcatggtc catgcccttg acccgaagga tccctcccgg ggccaggtcc
     1201 tcgtctgcga ggacatcgcc gagaaggtcc ggctcaccga aacggtcagg gagagcgatc
     1261 tccgggtccg cgaactcctc ctccatgcca gcagcctgat catcaagctc gatcccgatg
     1321 gcacgatcac cttcttcaac acctttgccc aggtcttctt cggctactcc gagaccgaga
     1381 tcatgggaaa gaagatcatc gggaccctga tcccgcaccg gaagggggag ggccaccagc
     1441 tgaccaggtt tgtctctgac atgggatcca acggggatgg ctttgccatc cgcatcaccg
     1501 agatgctgct ccgcgacggt cagccggcct gggtggcatg gaccaacaag gcgctccggg
     1561 atgaagacgg gcatatcctg gagatagtct gcatcggcca tgacatcacc gaccacgagc
     1621 accgggaccg gagccggatc agcaccgccc tctggaagga ccgggtgatc gcggactccg
     1681 acgtgaccga tgacgtattc gagacggtct tcaacatctg cctcgagatc gccaaagagg
     1741 gacgggaggg gaagaaggtc ggcaccgcgt tcgtgatcgg ggataccgaa aacgtcctct
     1801 ccaagtcaag gcagctgatc ctcaacccgt ttgccgggca ccggatcgag gaccggatgg
     1861 tcacgaacca cgatatccgc gagaatatca aggagctggc ccagctggac ggggcgtttg
     1921 tgatccgggg tgacgggctg atcgaggcag ccgcccggta catcacagtc gacacgagcg
     1981 ctgtcggcat cgccaagggg cttggcaccc ggcattcatc ggttgccggg atcacgctcg
     2041 tcacgaaagc gatcgggatc gtggtctccc agagcggggg gaagatctcg atcttccgga
     2101 acggccggat gctccaggag attgggtgaa ctgggggggc agtgccatcc acacctatta
     2161 atatcacccc ctacaaacac cacattgcag agaatcgtca ctgcatgagt aggaatgaat
     2221 aatcagagga agtatggcgg cccgtcatcg tccggaccgc ggaccatgac caagacggta
     2281 tgttcagatt gtggaaaaga gtgcgaggtt ccgttcaccc ccacccaggg aaggccggta
     2341 tactgcaggg actgtctccc taagcacagg aagccccgct attaatcatc tctttttttt
     2401 ctgatcctgg gaaagcttcg atcgattcat cccggttcca ggggacggca ggatccgccc
     2461 gggtaaaccg agggttgtat catccgggat cccgtgagag cgatccactc ctgcaggcat
     2521 gagagccggg caccaggtct gctgggggag catccccgcc cggatcaccc gaatagtcta
     2581 tttctgtgcg ggacccatca ttcaaccatg ggaaacagcc agcc
//
LOCUS       DAOOCO010000225         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39610_length_2622_cov_2.751850, whole genome shotgun sequence.
ACCESSION   DAOOCO010000225 DAOOCO010000000
VERSION     DAOOCO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39610_length_2622_cov_2.751850"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..492
                     /locus_tag="PK154_09875"
     CDS             181..492
                     /locus_tag="PK154_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HPW11407.1"
                     /translation="MFCPECKSLLMSSAGFLKCRKCGYNRKITDGDQLQRTRKRSEKE
                     IVIVEEEGQIHTLPTIAIKCPKCENNLAFWWLRQLRAADESEVRFFRCCECGHTWRQY
                     D"
     gene            768..1220
                     /locus_tag="PK154_09880"
     CDS             768..1220
                     /locus_tag="PK154_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11408.1"
                     /translation="MMLKRRLIVAGLLLLTVAAMGIASASEESFWAYYTQRYIEKFGE
                     DKFLLVNTPLDGLTYNSDYIEMHGEDKYALVTTPVMGDTYLKNYLTMHGQDKYALVTT
                     PLSPENDPANRWDEMHNAALARMPASTSWDERHKDVTSRLTPTFAWDY"
     gene            1460..2020
                     /locus_tag="PK154_09885"
     CDS             1460..2020
                     /locus_tag="PK154_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HPW11409.1"
                     /translation="MKVKPEDSLKIENIVASAKVTDYLDLPSLASQIKDAEYNKKRFP
                     GVVIRMQNPKIAALVFGSGKVVLTGAKSIESLSEGLEILGNLLRDLQIDIPQKLTYKI
                     QNIVTSADLGSGINLNKIAVGFNLDRIEYEPEQFPGLVYRLEDPKVVVLLFGSGKLII
                     TGGKVPEDAKRAVQKILNDLKNLGLL"
     gene            2168..2560
                     /locus_tag="PK154_09890"
     CDS             2168..2560
                     /locus_tag="PK154_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPW11410.1"
                     /translation="MTGVHLFRARNVMYFTQEEEDLADLLMKTGLKRNVARVLVYLAH
                     NPEATSREIERGTDLRQPEVSLAMAAMIDQNWVENREIKAENKGRPVKIYRLSRPIGE
                     ITDSIEKEKREEANLQLSIIQQIRTYIQ"
BASE COUNT          693 a          685 c          676 g          568 t
ORIGIN      
        1 gaagggacca ggaacgcaag gaaaaaaatg caggatgcga gcacgaagag ttcgttcatt
       61 cccataacaa tcgtcgcagg atgaaaaaaa gggttccgat tgggcatatt tgcccgttga
      121 aggtctgatc ggcgtatatc cggtgcctta atcatgaatg tgcaccaaat tattgaacca
      181 atgttctgtc ctgaatgcaa gagcctgttg atgtcctctg ccggcttcct aaaatgccgg
      241 aaatgcgggt ataacaggaa aatcaccgat ggagaccagc tgcagaggac gagaaaacgg
      301 agcgaaaagg agatcgtgat cgtggaggag gagggacaga tccataccct cccgaccatt
      361 gccatcaaat gccccaagtg cgagaacaac ctcgcgttct ggtggctccg ccagctccgt
      421 gccgctgacg agagcgaggt caggttcttc cggtgctgcg aatgcgggca tacctggcgc
      481 cagtacgact aaattttgct actggctaag tgtatcgtgc aggactcatt cgattttttt
      541 tgaacactcc gtcagttcat accgtaaaag cggtggcatc agcgtcatgg cggtccacag
      601 caacagagaa ccggctttcg atctcccgcc gtcactatgg tacaatacac gctattaatt
      661 gattgatggt ctatttctct tccatttagc ctgatgcagg gttcagatct ctttttggat
      721 gaaagagaaa agacccccct gcaggcgcga aaagagggag atgatccatg atgctgaaga
      781 ggagattgat tgttgcaggg ctgctcctgc tgacggttgc cgccatggga attgcatcgg
      841 caagcgagga atccttctgg gcgtactata cccagcgata catcgaaaag tttggcgagg
      901 acaagtttct cctggtgaac accccccttg acgggctgac gtacaactca gattacattg
      961 aaatgcacgg ggaagacaag tatgcccttg ttaccactcc ggtgatggga gatacgtatc
     1021 tcaaaaacta ccttaccatg cacgggcaag acaagtatgc actcgtcacc actccgctct
     1081 caccagagaa tgacccggcg aaccgctggg atgagatgca taatgctgca ctcgcccgga
     1141 tgccggcgag cacttcctgg gatgagcggc ataaggatgt cacttcgcgg ctgactccaa
     1201 cctttgcctg ggattactga gggaaggata caacctcccc cctgggttcc ctttcgccag
     1261 taacggagga tgccggccgg ggatactccc ttttcaccgg aattgcggca gcagccctct
     1321 ctcacaccag agagcggaga gcccgaacac acagccatcg ctggccgggt aaatcggcct
     1381 gcacgacaat cgccccggga atgattctcg gagcagcaac acccaactgt ttatatactg
     1441 tcctgtggca tttataaata tgaaggtcaa gccagaggac tctctcaaaa ttgaaaatat
     1501 cgtcgcttca gcaaaagtaa ccgattatct cgatctgcca tcactggcct cccagatcaa
     1561 ggatgcagag tacaataaga agaggttccc gggcgtcgtc atcaggatgc aaaatccaaa
     1621 gatcgccgca ttggtcttcg ggtcggggaa agtggtgctg acgggagcaa aaagcatcga
     1681 aagtctctcc gaggggcttg agatcctcgg aaaccttctc cgggatctgc agatcgatat
     1741 tccccagaag ctcacctaca agatccagaa cattgtgacg tctgcagacc tcgggtcggg
     1801 catcaatttg aacaagatcg ccgtagggtt caacctcgac cggatcgagt acgagcccga
     1861 gcagttcccc ggacttgtct accggctcga ggacccgaaa gtggtggtgc tcctctttgg
     1921 ttccggaaaa ctgatcatca ccggggggaa ggtgcccgag gatgcaaagc gggcggtgca
     1981 gaagatcctg aacgacctga agaaccttgg cctcctgtga tcccaggccg accaaaatcc
     2041 catccgacca aaatttttat ttccttcaga atgcctctac ctaataagga tttcttttcg
     2101 atttgtaata ctaaataata atctttttat ataataatta tctattaacc tatttacata
     2161 aatattgatg acaggtgtgc atttgttcag agcacgaaat gtgatgtatt tcacacagga
     2221 agaggaggac ctggcagacc ttctcatgaa gaccgggctc aagcggaatg ttgcccgggt
     2281 tctcgtgtat ctcgcccaca atcccgaggc gacgtcaagg gagatcgaac gcggcaccga
     2341 tctccgccag cccgaagtca gcctcgccat ggcggccatg atcgaccaga actgggtgga
     2401 gaaccgtgag atcaaggcgg agaacaaggg aagaccggta aagatctacc ggctgtcacg
     2461 cccgataggc gagatcactg attcgattga gaaggagaaa cgcgaagaag ccaacctgca
     2521 gctctccatc attcaacaga tacggaccta catccagtaa agtccaggga tacccccaat
     2581 accttactcc ctgtatcaat cccctccctc tctttccgga gg
//
LOCUS       DAOOCO010000226         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_39909_length_2608_cov_5.833529, whole genome shotgun sequence.
ACCESSION   DAOOCO010000226 DAOOCO010000000
VERSION     DAOOCO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39909_length_2608_cov_5.833529"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PK154_09895"
     CDS             <1..949
                     /locus_tag="PK154_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW11411.1"
                     /translation="RGQEVHVMHACGHDVHMAALIGAARILASREDWSGTLVMVVQPS
                     EETVSGAARMIADGLFSRFPRPDAAIALHVAPEIPAGTIGWREGIISAGGESLDIVVC
                     GIGGHAAHPERARDPVVLAAQIILAFQTIVSREVPPGELGIITVASVHGGSKHNAIPD
                     EVLLQVNIRYFRKEVRDLLLNAIKRVTEGIARSAGLAPPLFPRITLLPQSVPPMTNDP
                     ALTRKVADAFLRCYGEDHVLHIDPVTGSEDFGVFGLVEPRIPICYFRIGCGDEEYTCG
                     FLHSSRFAPPPEVIRDAAAALVVAAVAILDQAPPHPPGR"
     gene            complement(931..2439)
                     /locus_tag="PK154_09900"
     CDS             complement(931..2439)
                     /locus_tag="PK154_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW11412.1"
                     /translation="MPKYSFIARVPNHPGALHDAARVVMREKANINRVQFDQRIDPYI
                     VFFEVSCEEEAFGRIEKELAVMGYLKDTLRPLNILKFYVFLPHEPGALFEFLNHTTRH
                     KANISYIDFDDRGRHPERLTITVSLEENGSAEQLLDTLKSRYRIEIIEYDTTGKKLDD
                     TVFYIRFAQEIREIIGESEDPFLLSFLGDINHAVQELMNLGQDPKKIFGSFLATGRTL
                     TRTTGDGFYADIQTIPLSAGITLTCIQPPAGGNVYLINTPGGCTMVDTGYGIYSRDIG
                     TIIRNVIPGGRDAIRQIVITHADADHCGAAGEFPVPAAMHPGTLDIIRAANRAYGSRS
                     EASVLEEIYTTMINLFSKFNPPEEYRLFATRKGEGEAGFPVIGSIDIGGHQFTVLEGL
                     GGHMHGQIYLFSRELGVLFTADTVINFGHLTPERAEYNTLAVILVTSVNVDSGIARTE
                     RQNLLDLARRTTGSYHAGRTGCLVCCGHGPVSMIEGGVLLPFGNIEHYLPGG"
BASE COUNT          510 a          755 c          774 g          569 t
ORIGIN      
        1 ccgtggtcag gaggtacacg tgatgcatgc ctgcgggcat gatgtgcaca tggccgcgct
       61 gatcggtgcg gcacggatcc tcgcgtcccg tgaagactgg tccgggaccc tggtgatggt
      121 cgtgcagcca tcggaagaga ccgtttcggg ggctgcccgc atgatcgccg acgggctctt
      181 ttcacggttt ccaagaccgg acgctgcaat cgcattgcat gttgcccccg agattcccgc
      241 aggcactatc ggctggcgtg aagggatcat ctctgcggga ggagagtcgc ttgatatcgt
      301 ggtctgtggt atcgggggac atgccgctca cccggaacgt gcccgtgacc cggtcgttct
      361 cgcagcccag atcatcctgg ctttccagac cattgtaagc cgtgaagtcc cgcccggaga
      421 acttgggatc atcacggtgg catcggtcca tggcggaagc aagcacaacg cgatcccgga
      481 tgaggtgctg ctccaggtga acattcggta cttcaggaag gaggtcaggg acctgctgct
      541 caacgcgata aagagggtga cagaggggat cgcccggagt gccgggttgg caccaccgct
      601 ctttcccagg ataacgctcc ttccccagtc ggtcccgccc atgaccaacg atcccgccct
      661 cacccggaag gttgcagacg catttttacg atgctatgga gaggaccacg tgctccatat
      721 tgacccggtg accgggagcg aggacttcgg cgtcttcggg cttgttgaac cccgcattcc
      781 gatctgctac ttccgtatcg ggtgcggcga tgaagaatat acctgcggat tcctccacag
      841 cagccgtttt gcccctccgc ctgaagtgat tagggatgct gccgctgccc tggtcgttgc
      901 agcagtggcc atcctggacc aggccccccc tcacccaccg ggcaggtagt gctcgatatt
      961 tccgaacggg aggagcactc ctccctcgat catggagacc gggccatgcc cgcagcagac
     1021 caggcatccc gtcctccctg cgtggtagga accggtggtt ctccgggcaa gatccaagag
     1081 gttctgccgc tcggtcctgg ctatgccgct atccacgttc actgaggtga ccaggatgac
     1141 ggcaagcgtg ttatattcgg cccgttcggg ggtgaggtgc ccgaagttga tcacggtatc
     1201 agccgtgaag agaaccccaa gctcccgtga gaacaggtag atctgaccgt gcatatgtcc
     1261 gccaagcccc tcgaggaccg tgaactggtg tccccctata tcgatagatc cgattacggg
     1321 aaatcctgcc tctccctcgc ccttccgggt tgcgaagaga cggtattctt ccggcgggtt
     1381 gaacttcgag aagaggttga tcatcgtggt atagatctct tcgaggacgg atgcctcgct
     1441 cctcgatcca tacgcccggt tcgcagcccg gataatatcc agggttccgg gatgcatcgc
     1501 ggccggcacc gggaattccc ctgctgctcc gcagtgatcg gcatcggcat gcgtgatcac
     1561 gatctgccgg atggcatccc gcccgcccgg gatcacgttc ctgatgatcg tcccgatatc
     1621 ccgggagtag atcccatagc cggtatcgac catcgtgcag ccccctgggg tgttgatcag
     1681 atagacattg cctccggcag gtggctggat gcaggtgagg gtgatcccgg cgctgagcgg
     1741 aatggtctgg atatctgcat aaaatccgtc cccggtcgtc cgggtgaggg tccggccggt
     1801 cgcaaggaaa ctcccgaaga tcttcttcgg atcctgcccg aggttcatca gctcctggac
     1861 cgcatggttg atgtcgccaa ggaacgagag gaggaacgga tcttccgatt ccccgatgat
     1921 ctcacggatc tcctgggcaa accggatata aaagaccgtg tcgtcgagtt tctttcccgt
     1981 ggtatcatat tcgatgatct cgatccggta gcgggacttc agggtgtcga gcagttgttc
     2041 tgctgaaccg ttctcttcaa gactgacggt gatcgtgagc cgctcagggt ggcggccacg
     2101 atcatcaaag tcgatgtatg agatgttcgc cttatggcgg gtagtgtgat tcaaaaactc
     2161 gaagagggcg ccgggttcat gggggagaaa gacataaaat ttcaggatgt tcaggggacg
     2221 gagcgtgtcc ttgaggtatc ccatcactgc gagctccttc tctattctcc cgaatgcctc
     2281 ctcctcgcat gatacctcaa aaaagacaat atacggatcg atccgctggt cgaactgcac
     2341 gcgattgata tttgccttct cgcgcatcac gacccgggct gcatcatgga gcgccccggg
     2401 atggttcgga acccgcgcaa taaacgagta tttcggcatc gtatctctct gtatctgatg
     2461 tgtggcatac gtattcgatc tgaaaggaca tctctcttcc tgctcggaac tctcttcctg
     2521 cggggatatc ctcttgccgg tccgggagga aggctctcat cgcaccaggg tatcctgatg
     2581 acaagccgaa aagagctggt caatgaag
//
LOCUS       DAOOCO010000227         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_40279_length_2591_cov_5.899842, whole genome shotgun sequence.
ACCESSION   DAOOCO010000227 DAOOCO010000000
VERSION     DAOOCO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40279_length_2591_cov_5.899842"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1655
                     /locus_tag="PK154_09905"
     CDS             129..1655
                     /locus_tag="PK154_09905"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPW11413.1"
                     /translation="MVFFTDSQAKKHVTVFDTTLRDGEQTPGISFTFAQKLEIARQLA
                     SIGVHAIEAGFPASSQGEQETVKAIADQRLDAVICGLARSRTEDVDVCIDCDVDMVHV
                     FIPTSEVQRVHTIKKTPEEVLDITREIVSYVRDHCELCMFSAMDATRTDTEYLIEVFR
                     AAADAGATIINVPDTVGVYTPTAMKKLITRIVSEVNCPVDVHCHNDFGLAVANTTTAV
                     EAGASQVQVTVNGLGERAGNADISQTVMILESIYGVKTGIKTASLYETSRLISRYSGI
                     GVLPIQPVVGDNAFSHESGIHSHGVIACAATFEPGIMTPEMVGHRRRLTLGKHVGRHA
                     VRQMLADAHINPSENEIDQIVEKVKFMSVKGKKVTDADLYEIATTIMGIGNGGQAIEL
                     QDIAIMTGNHVIPTASVKALVNGKELVCSSTGNGPVDAALKAILGIVPAKVQLKEFNI
                     EAISGGSDAIGHVNIAIEDDHGHVFSASGSGDDIVLASTEAMVNAINLLQRIRQAGTN
                     "
     gene            complement(1679..2038)
                     /locus_tag="PK154_09910"
     CDS             complement(1679..2038)
                     /locus_tag="PK154_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HPW11414.1"
                     /translation="MSSISPKVQNQIAMLQQMQQQMQTIGAQKAQYEMAVREAKRAIE
                     ELNDVAEDAPVFMSVGTVMMEKKREDVLNNLNNKIETLELRIKSLEKQEGMLSGKFEQ
                     LQSQIKQALEGRPQSAS"
     gene            complement(2035..2292)
                     /locus_tag="PK154_09915"
     CDS             complement(2035..2292)
                     /locus_tag="PK154_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HPW11415.1"
                     /translation="MRHEALFRFRSAHAEPLFHAVRPEMEGECNPRSKAMCLLEPPDT
                     LVLAVHAADIAALRASLNMWLRLILVAEEMQELLNNQETIQ"
     gene            complement(2282..>2591)
                     /locus_tag="PK154_09920"
     CDS             complement(2282..>2591)
                     /locus_tag="PK154_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11416.1"
                     /translation="KAGLAEITSRDTNIIIFSLYYGTLPSFTLYTEGKQGTLFLVSDL
                     KTIQRDGPRLGIQVNDPSIYERLVPYIPVKLSEDGAETCIIDGIRSRRYLLSLKIHEA
                     "
BASE COUNT          640 a          662 c          664 g          625 t
ORIGIN      
        1 atagtatagg ggaaataaag ctattaaaca tatcgatatc ggctcctgaa aatggccttt
       61 cggtcactgt tttataatat gagcgcattt taaatggtgt atggttccca taacaactga
      121 agcggggaat tgttttcttc accgatagcc aagcgaagaa acatgtgact gtttttgata
      181 ccaccctccg ggatggggaa cagacgccgg gtatatcatt tacctttgca cagaagcttg
      241 agatcgcccg ccagctggca tccatcgggg tgcatgcaat cgaagccggc ttcccggcct
      301 cctcccaggg agaacaagag acggtgaaag ccatcgcgga ccagagactc gacgcagtta
      361 tctgcggcct ggcccgatcc cgaacggaag atgttgacgt ttgcatcgat tgtgatgttg
      421 acatggtaca tgtcttcatc cccacctctg aagtccagcg ggtgcacacc atcaaaaaga
      481 ctcctgaaga ggtgcttgat attacccggg agattgtctc ctacgtccga gaccattgcg
      541 aactttgtat gttctcagca atggacgcca ccaggactga tacggaatac ctgatcgaag
      601 tattccgggc cgctgctgat gcaggggcaa ccatcatcaa cgtcccggac acagtcgggg
      661 tctacactcc cactgcaatg aaaaagctga tcaccaggat tgtgagtgag gttaactgcc
      721 ccgtcgatgt ccactgccac aacgattttg gccttgccgt ggccaatacg accaccgcag
      781 tggaagcagg ggcatcacag gtccaggtaa cagtgaatgg actcggggag cgtgccggga
      841 atgcagatat ttcccagacc gtcatgatcc tggaatccat ttatggagta aaaaccggga
      901 tcaagacagc cagcctgtat gagacgtcac ggttaatctc ccgttattcg gggattggcg
      961 ttctcccgat ccaacctgtt gtcggtgaca atgccttctc tcacgagagt ggaatccatt
     1021 ctcacggcgt cattgcgtgc gctgcgacat ttgaaccggg tataatgacc cctgaaatgg
     1081 tagggcaccg gagaaggctc acccttggca agcatgtcgg acgccatgct gtccgtcaga
     1141 tgctggcaga tgcgcatatc aatcccagtg agaacgagat tgatcaaatc gtagagaagg
     1201 tcaaattcat gtccgtgaaa ggcaagaaag tgactgatgc cgatctctac gagattgcga
     1261 cgaccatcat gggcatagga aacgggggac aggcaatcga actccaggat attgctatca
     1321 tgaccggaaa ccatgtcata ccaacggcga gtgtaaaggc actcgtcaat ggaaaggagc
     1381 tggtatgcag cagtaccgga aatgggcctg tcgatgctgc cttgaaagcg atcctcggta
     1441 ttgtacctgc aaaggtgcag ttaaaggagt tcaacatcga ggcgatttcc ggtggatctg
     1501 atgcaatcgg gcatgtgaac atcgccatcg aggatgacca cgggcatgtt ttctcggcga
     1561 gtggatcagg tgacgatatc gtacttgctt ccactgaagc gatggtaaat gccatcaacc
     1621 tgcttcaacg gatacggcag gctggaacta attaaaaaga agtgaacagg ccgcgagatc
     1681 aggatgcgct ctgaggcctg ccctcaagcg cctgtttgat ctggctctga agctgctcga
     1741 acttgccgga gagcatcccc tcctgttttt caagcgattt aatcctaagt tcgagcgtct
     1801 ctatcttatt gttgagattg ttcaggacat cttctctctt cttctccatc atgactgtgc
     1861 cgacgctcat gaaaaccgga gcatcctctg ctacatcgtt cagttcctca atagcccgtt
     1921 tcgcctcgcg gaccgccatc tcgtactggg ctttctgggc accaatggtc tgcatctgct
     1981 gctgcatctg ctgcagcatt gcgatctgat tctgcacttt cggtgaaatt gaactcattg
     2041 tattgtctcc tgattgttca gcagttcctg catctcttct gccaccagaa tcagccggag
     2101 ccacatgtta agcgaggccc gaagggctgc aatatccgct gcatgcaccg ccagtacaag
     2161 ggtatccggc ggctcaagga ggcacatcgc ctttgatcgg ggattgcact ctccttccat
     2221 ctctggacgc accgcatgga aaaggggttc agcatgtgct gacctgaacc ggaagagtgc
     2281 ttcatgcctc atggatcttc agtgaaagca ggtaacggcg tgatcgtata ccatcaatga
     2341 tacacgtttc cgcaccatct tctgacaatt tcaccggtat atatggtacc agccgctcat
     2401 aaatggatgg gtcatttacc tggattccca atcgtggccc atcccgttga atcgtcttta
     2461 gatccgatac aaggaacaat gttccctgtt tcccctcggt gtacaatgtg aaagagggta
     2521 aggttccata gtaaaggctg aaaataataa tattggtatc acgtgacgta atttctgcaa
     2581 gccccgcctt g
//
LOCUS       DAOOCO010000228         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_40754_length_2570_cov_5.222664, whole genome shotgun sequence.
ACCESSION   DAOOCO010000228 DAOOCO010000000
VERSION     DAOOCO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40754_length_2570_cov_5.222664"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /gene="hisG"
                     /locus_tag="PK154_09925"
     CDS             <1..599
                     /gene="hisG"
                     /locus_tag="PK154_09925"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW11417.1"
                     /translation="FGNATLVVAVKEEDHARKIQDLAGRKIATEFPVITENLFRGEKI
                     PITVVPVGGACEVTPYLGIADAILDLSSSGDTLRSNRLRVIGKVLDTSTILIGNRDSM
                     VSKSDKIDEVVLALESVVRARGQCYLMMNAKRSCLEEITNVLPGLSGPTVMEVASSDD
                     LVAVHAVVSEDRVYRLINRLKRAGARDILVMSIERMIR"
     gene            601..1320
                     /gene="hisA"
                     /locus_tag="PK154_09930"
     CDS             601..1320
                     /gene="hisA"
                     /locus_tag="PK154_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPW11418.1"
                     /translation="MKIFPAVDILSGRCVQLVQGRRDTATSFGRPIECALRWINAGAR
                     QLHVVNLDGAFGNSAANLPLIREIIRNTGVTIQLGGGIRSFEDAAGWLDAGVDRVIIG
                     TLAVKDPSVLTDLSDHYGRSRIIAGVDARGGLITIDGWDRTEGDYIRWARIFEEKGAG
                     TLLYTNVDVEGLQRGIRLPPVRRLIESVGIPVIVAGGISSSNDVRVLKEIGAYGVVLG
                     SSLYSGMLSLREALEVADEED"
     gene            1307..1888
                     /gene="hisB"
                     /locus_tag="PK154_09935"
     CDS             1307..1888
                     /gene="hisB"
                     /locus_tag="PK154_09935"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPW11419.1"
                     /translation="MRRTEVSRRTKETSITIVLDLDGSGKVQAGTGLPFLDHMLEAMA
                     RHGRFDLECTAAGDLEVDSHHTVEDVGLVLGEAIKKTIGEGRGIKRFAHAIVPMDESL
                     ATVALDCSGRGFLIFQGGLSHPALGSIERDSFEHFFQSLCGKAGLNAHIIFHGRNDHH
                     QMEAVFKAFGIALGEAAAIVPGREDVPSTKGLF"
     gene            complement(1917..2288)
                     /locus_tag="PK154_09940"
     CDS             complement(1917..2288)
                     /locus_tag="PK154_09940"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPW11420.1"
                     /translation="MTIEKPDTVELTRGETTIKGGCDNQSSLLIFYRTHPVPEDSVPG
                     DDSNADLSNRIGSDFNSLIGLLSHEEPDIRITAARALGKQGDPNAIEPLFKACMDENC
                     GVKSAARDALALIVAQMHSHK"
BASE COUNT          605 a          608 c          704 g          653 t
ORIGIN      
        1 gattcgggaa tgccactctc gtggtcgcag taaaagagga ggaccatgcg aggaagattc
       61 aagaccttgc cgggagaaaa atcgcaacag aattccccgt aatcaccgaa aatctcttca
      121 ggggcgagaa gattcccatc actgttgtcc ctgtcggtgg tgcctgtgaa gtgacgcctt
      181 atcttggaat tgcagatgcc atccttgacc tctcaagctc cggagacacc ctcaggtcca
      241 accggctgcg tgtgatcggg aaggtgcttg acaccagtac catccttatt ggaaaccgcg
      301 attcgatggt gtccaagtct gataagatcg atgaagtggt gctggccctt gagagcgtcg
      361 tcagggcgcg ggggcagtgc tatctgatga tgaatgcaaa acgctcctgc ctcgaagaaa
      421 ttaccaatgt gcttcccggt ctctccggcc cgacggtcat ggaggttgct tcatctgatg
      481 atcttgttgc ggttcatgca gtggtgagcg aggaccgggt atatcgcctg atcaaccgtc
      541 tgaaacgggc gggtgcaaga gatattcttg tcatgtccat cgagcggatg attcgctgat
      601 atgaagatat ttcccgctgt cgacatactc tcgggcaggt gtgtccagct cgtgcaaggg
      661 agaagggaca ccgctacctc ttttggccgg ccaatcgaat gtgccctgcg atggatcaat
      721 gccggcgccc gtcagctcca tgttgtaaat cttgatgggg catttggcaa ctctgccgca
      781 aaccttcccc tgatcaggga gattatccgg aataccgggg tgacgattca gctgggaggt
      841 ggcattcgct catttgagga cgcggctggg tggctggatg caggagttga ccgtgttatc
      901 atcgggaccc tggcggtaaa agatcccagc gttcttactg acctttctga tcactatggt
      961 cgaagcagaa ttatcgccgg agtcgatgcc cgagggggat tgatcactat cgacggctgg
     1021 gaccgtactg agggcgatta cataaggtgg gcacggattt ttgaggagaa gggtgccgga
     1081 acactgctct ataccaatgt cgatgtcgaa ggactccagc gtggcatacg gcttccaccg
     1141 gtcagacgcc tcatcgaatc ggtcggaata ccggtaattg tggcaggggg catctcaagc
     1201 agtaacgatg tgagagtatt aaaggagatt ggggcatatg gagtagtatt gggctcatcg
     1261 ctctattcag ggatgctctc cctgcgcgaa gccctggagg ttgccgatga ggaggactga
     1321 ggtatcacgc aggacaaaag agacgagcat cactatagta cttgatctgg atgggtcagg
     1381 aaaagtccag gcaggaaccg gcctgccgtt cctggatcat atgcttgagg caatggcccg
     1441 ccatggcagg tttgacctcg aatgcacggc tgccggagac cttgaagtag attcacatca
     1501 cacggtagag gatgttggcc tggtccttgg agaggcgatc aaaaagacta ttggagaggg
     1561 tcggggcatc aaaaggtttg ctcatgctat cgttcccatg gatgaatcac ttgcaacagt
     1621 tgcccttgac tgcagcggaa gaggatttct cattttccag ggaggtcttt cacatccggc
     1681 actgggctcc atcgaaagag acagctttga acatttcttt cagagtcttt gcgggaaagc
     1741 tggcttgaat gcccatatca tctttcatgg gaggaatgat catcatcaga tggaagctgt
     1801 gtttaaagcc ttcggcattg cacttggtga ggctgctgca atagtcccgg gaagagaaga
     1861 tgtcccgagt acaaaagggc tcttctgata cgtattgtct ggtttccgga gccgtttcat
     1921 ttgtgactgt gcatttgtgc gacaataagc gccaatgcat ctcttgcagc agatttcact
     1981 ccacaatttt catccatgca ggctttgaat aacggctcaa tcgcattggg atctccctgc
     2041 ttgccaaggg ctctcgcggc agtgatcctt atatcaggtt cttcatgact gagcaaccca
     2101 attaatgaat tgaaatccga tccaatcctg ttgctgagat cggcgttgga atcatcgccc
     2161 ggtaccgaat cttccggaac cggatgcgtc cgataaaaaa tgagtaagga ggactggtta
     2221 tcacatccgc cttttatggt agtttccccc ctggttaatt cgactgtatc cggcttttca
     2281 atggtcaaat tactccccag cccctcatac gagggtacta ccaactttca ctccatccta
     2341 aaaattcata cgccattcac ctatgcacgg cattatttct gacggattaa accaaattat
     2401 tctataatgt gaatatttgg gaaaagggca tcccggatag tatgatgctt agcgaaaaga
     2461 agatgtgcca ccttggtttt gattgctgct gtttgttcgt ttgccgtccg tgaaggaatt
     2521 gatggagtta tgcggacttt accgcaagat ctacatcttc ttccttgatt
//
LOCUS       DAOOCO010000229         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_41213_length_2552_cov_4.192231, whole genome shotgun sequence.
ACCESSION   DAOOCO010000229 DAOOCO010000000
VERSION     DAOOCO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41213_length_2552_cov_4.192231"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..756
                     /locus_tag="PK154_09945"
     CDS             577..756
                     /locus_tag="PK154_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11421.1"
                     /translation="MSKRAIDAVFKGLFLLTDIRVMLRETAPQHALDESQREKVRSLL
                     DALEKEVAVLREELA"
     gene            756..1937
                     /gene="pscS"
                     /locus_tag="PK154_09950"
     CDS             756..1937
                     /gene="pscS"
                     /locus_tag="PK154_09950"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HPW11422.1"
                     /translation="MKCTGTIDVRDVDESSINIDPIQVGGRLTPDAMKAMIAYGDGYS
                     VCDQCRKPFRLDYINTPPIAQFHRDLASFVNMEIARVVPGARRGFQAVADAFISPGDP
                     VLITALSHYTEVLAIEGRGGVIREIPKTGSNIITSELAAQKIEEVTREFHRSPTLLFL
                     DHFDYQYGNCHDVAGIIRVAHQYDVPVLYNGAYTVGIMPVDGKKIGADFVVGSGHKSM
                     AAPAPSGMLATTSEWAGRVFRTTGVTTDVTKRTFGIKEIGMMGCTLMGVTLMGMMASF
                     PHVRERVTHWEEELANGRKVVDALRSIEGTVIKSEYPRNHTLTRVDTTGSFDRVAQVH
                     KKRGFFFSSALKGKGIAGIIPGATKVWKFNTYGMTDSQAEYLATAFRDIGRENGLAVT
                     D"
     gene            complement(1927..>2552)
                     /locus_tag="PK154_09955"
     CDS             complement(1927..>2552)
                     /locus_tag="PK154_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPW11423.1"
                     /translation="QLSRILSHYRCQAGGQVILLIDLCYRNGSLGFDEFVRPVARIVR
                     HSGDDAIPVHFRCLTDPLLSQADAVILCGTPLADTAFLSEAGAFSWLKETSLPVLGIC
                     AGMEVICTTFGGTLGPCTEIGMTDVSVTENDPLFQESGSFSAYELHTLACATLKDLEV
                     LAVSNHCVQVVRHPEKTIYGVMFHPEVRNDWVIERFLTCSVEGIISP"
BASE COUNT          665 a          640 c          705 g          542 t
ORIGIN      
        1 tccatcagaa aggttgagaa aaccatcgag acgaacaaga acgacaagaa aaaatcgtga
       61 accggggaga tcccggtctt caatcttctt aaaagggaac ggttccatcc gatcatcacg
      121 aacacgccaa ccctgacttt tttacccata gcactatcct ggatatcaca tacccttctt
      181 tggcgggaaa aagaacgggt acgaagaggg cggtgctttc tatcaatctt cagtgcagtg
      241 atgctgaatg tgcgcctggg acagagcgat aaccattgtg ttcaggtatg gggaagactg
      301 agggccccga tccattaaaa aattgactgt ttcccacatc tccctccgta atgcctcctg
      361 tcctgaaaaa agtcccgcag acgattcatt gaccgatttg agaagcaggc accgcattga
      421 gtctaaccgt gtcaaataga gaagcagaga cggacaggaa ccgcacctgc tactatgacc
      481 ccaagaatag tcttaaggga gctgtggtga aaatctgtga aaccagttct atcggttatc
      541 ttataggtac attcctacta ttattatggt gatttcatgt caaaacgggc catcgatgct
      601 gtttttaagg ggctcttcct tctgaccgac atcagggtca tgctcagaga aactgcgcct
      661 cagcatgctc ttgacgagag ccagcgggaa aaggtccgct ccctccttga tgcactggaa
      721 aaagaggtgg cagtattgcg ggaggagctt gcctgatgaa atgcacaggg acgatcgatg
      781 taagggatgt tgatgaatcc agcatcaaca tcgacccgat ccaggtagga ggacggctca
      841 ctcccgatgc aatgaaagcg atgattgcgt atggtgacgg gtattcagtc tgtgaccagt
      901 gcaggaaacc attcaggctg gactatatca atacaccccc catcgctcaa ttccatcgtg
      961 accttgcctc tttcgtcaac atggagatcg caagggttgt cccgggggcc cggagagggt
     1021 tccaggccgt ggccgatgca ttcatctctc cgggcgatcc ggtcctcatc accgccctct
     1081 cccattacac cgaagtgctt gcaatcgaag ggaggggagg agtgatcagg gagatcccaa
     1141 aaaccggatc aaatatcatc acctccgagc tggccgccca gaagatcgag gaggtgacaa
     1201 gagagttcca tcgttcacca actctcctct tcctcgatca tttcgattac cagtatggga
     1261 actgccacga tgtcgcgggg attatccggg tagcccacca gtatgacgtc ccggtgctct
     1321 ataacggtgc ctacacggtc gggatcatgc cggtggatgg aaagaagatc ggtgccgact
     1381 ttgttgtcgg atccgggcat aagagcatgg cagcaccagc accttccggc atgctcgcta
     1441 caacctcgga atgggcggga cgggtattcc ggaccacggg tgtcacgacc gatgtgacca
     1501 agagaacgtt cgggatcaag gagatcggca tgatgggttg cacattgatg ggcgtcaccc
     1561 tgatggggat gatggcgtcg tttcctcatg tcagggaacg ggtgacccac tgggaagagg
     1621 agctcgccaa tgggcggaag gtggtcgatg cgctcaggtc gatcgagggg acggtgatta
     1681 agagtgagta cccaaggaac cataccctga cccgggttga caccaccggt tcatttgacc
     1741 gggtggcaca ggttcacaag aaacgtggat tcttcttctc aagcgctctg aaaggaaagg
     1801 gaatagcggg aatcatcccg ggagcaacaa aggtatggaa gttcaatacg tatggaatga
     1861 cggattcaca ggcagaatac cttgctacag ccttcaggga tatcggaagg gagaatggtc
     1921 tggctgttac ggactgataa taccctcgac tgaacaggtg aggaaccgct cgatgaccca
     1981 atcatttctc acctccgggt gaaacatcac cccgtatatt gttttttcag gatgccggac
     2041 gacctggaca caatggtttg atacggcgag aacttccagg tccttcaggg tggcgcatgc
     2101 gagagtgtgg agctcgtaag cagaaaatga tccggattcc tggaacagag ggtcgttttc
     2161 ggtcacggag acgtcagtca tgcctatctc tgtacatggc ccgagagttc ctccaaaggt
     2221 ggtgcagatc acttccatgc ctgcacagat tccaaggacc ggcagactgg tctccttcag
     2281 ccaggaaaaa gcaccggctt ctgaaagaaa ggcggtatca gcgagcggtg tcccgcagag
     2341 tatgacagcg tcagcctgtg agaggagcgg atcggtcagg caacggaaat gcacaggtat
     2401 agcgtcatct cccgaatggc gcacaattcg ggcaaccggc ctgacaaact cgtcgaatcc
     2461 cagagaacca ttacgataac agagatcgat caaaaggatc acttgcccac cagcctggca
     2521 ccggtaatgc gataaaatcc gcgataattg tg
//
LOCUS       DAOOCO010000230         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_41505_length_2541_cov_4.902253, whole genome shotgun sequence.
ACCESSION   DAOOCO010000230 DAOOCO010000000
VERSION     DAOOCO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41505_length_2541_cov_4.902253"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK154_09960"
     CDS             complement(<1..1143)
                     /locus_tag="PK154_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPW11424.1"
                     /translation="MEVTNQQVAAQLNLMGQILEIKGDNVFKIRAFYRASESIERLAT
                     PVTAMDDTALSGIEGIGKNIVRKVREIAEKGTFDELEEAKSGIPERLIELLHLEGIGP
                     KTVSTLWKKMNIQSIDDLERAAKGHRIRAIKGFGEKKEEGFLKAITMYRDQSGRMNRE
                     EADAVAGKIRPVLEPGTYEFAGSYRRGKSSVGDIDVVTTESPDHLNPRLRAIADAVID
                     AGDRKTSLRILGKRVDVRFSKPRQFGSMLLYLTGSKAFNIKLREIAISRGYKLNEYGI
                     EDRSDGSLWEFSTEAELLSFLGLDYVVPELREDWGEVERALSHALPPLVEQDDIRGDL
                     HVHSTWSDGSLSIQDLGTSGEERGYEYIVCSDHSSTLGIAHGLDEDA"
     gene            1299..1961
                     /locus_tag="PK154_09965"
     CDS             1299..1961
                     /locus_tag="PK154_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HPW11425.1"
                     /translation="MSNHLCMETTSLREDILEALYKIHFSGRVAPVVEDLATMLGKSP
                     GELRQVLDLLEKGGTLVSNPDGTLTLTPTGSEIGGRVMRKHRILECFFSEMLGMSPDT
                     ASEEACTLEHGVSDEAIERLGRYIEKRPGRGAGYRRTRKGKRWQALSLAEADEGDTLT
                     VAGVRCEGPGTRLNDLGLFPGTEISVVRKIPGNGIVVRVKDCNIALSQEVAAFVLVER
                     TG"
     gene            1958..>2541
                     /locus_tag="PK154_09970"
     CDS             1958..>2541
                     /locus_tag="PK154_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HPW11426.1"
                     /translation="MKVGLIGNPNVGKSVIFHQLTGVGVEVSNYPGTTINRQAGTTCF
                     QRAIIEFIDLPGTYSLEGDSEEEKIVRSFIDSDEAEVIVAVLDAGRLERNLYLTLQIA
                     EYQKPMIVVLNMMDEAEKSGIHVDTEQLSTILGVEVIPTVATQGKNTGSIIPAVINAA
                     RIPEGKVLYDHHIEAAIKTLRTVHALTWIRAIHAL"
BASE COUNT          574 a          713 c          658 g          596 t
ORIGIN      
        1 agcatcctca tcaagaccat gggctattcc caatgtggat gaatggtcgg aacaaacgat
       61 atattcatac cctcgttcct ccccagatgt tccaaggtcc tggatggaaa gactaccatc
      121 gctccaggta ctgtgaacat ggagatctcc cctgatatca tcctgctcta caaggggagg
      181 cagggcatgg gacaacgccc gttccacctc accccagtcc tcccgaagct cgggaactac
      241 atagtcaaga ccgaggaacg acagcagttc cgcctcagtg gagaactccc aaagcgaccc
      301 gtcagatcga tcttcaatac catactcgtt cagcttgtaa ccacggctta ttgcgatctc
      361 cctcagtttt atgttgaatg ctttcgagcc ggtcaggtag aggagcatgg atccaaactg
      421 tcgcggcttt gagaaccgga catccactct ctttccaagg atccgaaggg aggtcttccg
      481 gtcgcctgcg tcgatcaccg catccgcaat ggcccgcagc ctgggattca gatgatcggg
      541 ggattcggtt gtcaccacat cgatgtcccc cacactgctc tttcccctcc ggtaactgcc
      601 ggcaaactcg taagtacctg gttcaaggac gggtcgtatc ttcccggcca ctgcatctgc
      661 ctcctcacgg ttcatcctcc ctgactggtc ccggtacatt gttatggctt tgaggaatcc
      721 ctcctctttt ttctctccga atcccttgat tgccctgata cgatgacctt ttgctgcccg
      781 ttccagatcg tcgatactct ggatattcat cttcttccag agggtagaga cggttttcgg
      841 gccgattccc tcaagatgca gtagttcgat cagtctttcg gggatcccgg attttgcttc
      901 ttcgagctca tcaaaggtcc ccttttcagc aatttcacgg accttccgca caatattttt
      961 cccgatccct tcgatccccg agagtgccgt atcatccatg gctgtaaccg gcgttgcaag
     1021 tctctcgata ctctcggagg cccggtagaa ggcccggatc ttaaagacat tatccccttt
     1081 tatctcgagg atctggccca tgaggttcag ctgtgccgca acctgctggt ttgtcacctc
     1141 catctggccg gatcactcct cctgcacttc ttatcctctt cagttgatca gttcccctga
     1201 tatgcatttc ttccctgacg tcgtccggga agaaacgacc tgataccggg aaaaactgtt
     1261 gatcaggaga tgtatcatcc aaattcataa gtgcatccat gtcgaatcat ctttgcatgg
     1321 agaccacctc cttacgggaa gatattcttg aagctctcta caagatccat ttctctggca
     1381 gggttgcacc cgtagtggag gacctggcta cgatgcttgg aaaatcaccc ggggaactcc
     1441 ggcaggtact cgaccttctt gagaagggag ggacccttgt atccaacccg gacggcacgc
     1501 tcaccctcac ccctacaggg agtgaaatag gcgggagggt gatgcggaag cataggatcc
     1561 ttgaatgttt cttttcagag atgctcggaa tgtccccgga cactgcatcg gaggaggcat
     1621 gtacgcttga acatggcgtc tctgacgagg caatcgaacg tcttgggaga tatatcgaga
     1681 aacgccctgg taggggagca gggtaccgca gaacacggaa ggggaagcgg tggcaggcgt
     1741 taagcctggc cgaggcagac gagggggata cccttactgt cgcgggcgtg cggtgcgagg
     1801 gtccgggaac ccgcttgaac gatctcggcc tgttccccgg cactgaaatc agcgtggtcc
     1861 ggaagattcc cggaaatgga atcgtggtaa gggtcaagga ttgcaatatt gctctgagcc
     1921 aggaagttgc agcttttgtc cttgtggagc ggaccggatg aaggtcgggc tgatcgggaa
     1981 tccaaacgtc ggcaaatcag tgatattcca ccagctgacc ggggtcggag tggaggtcag
     2041 taattacccc gggaccacca tcaaccggca ggccgggacc acctgttttc agagggcaat
     2101 catcgaattt atcgatctcc ctggcaccta ttccctggaa ggagattctg aagaggaaaa
     2161 gatcgttaga tcctttattg attcggacga agctgaggtc atcgtcgcgg tgctcgatgc
     2221 cgggcgcctg gagcggaacc tctacctcac gctccagatc gctgagtacc aaaaaccgat
     2281 gattgtcgtc ctcaacatga tggacgaggc agaaaaatcc gggatccatg tggatacaga
     2341 acaactctcc actattctcg gtgtcgaggt aatacccacc gttgccacgc aggggaagaa
     2401 taccggcagt atcatccctg ccgtgatcaa tgccgcacgg ataccggagg ggaaggtcct
     2461 ctatgatcac catatcgagg ccgcaataaa aaccttgaga accgtccatg ccctcacctg
     2521 gatcagggct atccatgccc t
//
LOCUS       DAOOCO010000231         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_42561_length_2500_cov_3.977914, whole genome shotgun sequence.
ACCESSION   DAOOCO010000231 DAOOCO010000000
VERSION     DAOOCO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42561_length_2500_cov_3.977914"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK154_09975"
     CDS             complement(<1..764)
                     /locus_tag="PK154_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPW11427.1"
                     /translation="MDALDRALQRSGAAAYVMTGSSEYADMRYLTRFTTTDPIIYVRS
                     AGERGWIIVSQMEYTRAQQEAITRVMTRADAGLFRILEEEKDRWRALARMVANLVEGA
                     ILIPPDFPYALGRHLDALTGVMTDTGTVSLMRAKKSRKEVIAIEKVQRSAERAMLHAI
                     DSIRRSKSRNGMLWLGGKPLTSERIRSEMHALLIHEGCIARDTILSCGKDTAMPHNKG
                     TGPLFEDEPIVIDIFPRSEQTGYHSDMTRTVVKGNP"
     gene            1096..1383
                     /locus_tag="PK154_09980"
     CDS             1096..1383
                     /locus_tag="PK154_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11428.1"
                     /translation="MIEKVKSEIELMQRHIQVMRTVVDHEPIGIMKLSEILDLPFHRI
                     RYSLRVLEQMGYIRASPSGAMATDLAADLLEHLDDEIDEMVRLLEGMKKAH"
     gene            1454..1526
                     /locus_tag="PK154_09985"
     tRNA            1454..1526
                     /locus_tag="PK154_09985"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Asn,seq:gtt)
     gene            1536..1610
                     /locus_tag="PK154_09990"
     tRNA            1536..1610
                     /locus_tag="PK154_09990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Ile,seq:cat)
     gene            1812..>2500
                     /locus_tag="PK154_09995"
     CDS             1812..>2500
                     /locus_tag="PK154_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11429.1"
                     /translation="MKCPVCGDDCVKEAHEIIRMIPAVFARCQDCRFKILDKSLPPPP
                     DANLEACRCGRRFIDEVFAHLYALLVREGVFTGKEALKEVGSPLLHPGYPLTTPPFLP
                     KDSLVLLSRHPTRAVAQMMVDEIPEVRGVVRSGDYIPGVIDPSLNSPPCTYDLLAGCD
                     VRANVFPTKMGPVVLYQQQSTIHIELPRRHNPKITSVEERIARIRPEWFVDACCGAGT
                     LGITAARLGVP"
BASE COUNT          551 a          672 c          633 g          644 t
ORIGIN      
        1 caggggttac ccttcacgac cgtccttgtc atatctgagt gatatcctgt ctgttcgctc
       61 cttggaaaaa tatcgatcac gatgggctca tcttcgaata acggcccggt gcccttgtta
      121 tggggcatag cagtatcctt cccgcaggag aggatggtat ccctggcaat acagccctcg
      181 tgtatcagca gcgcgtgcat ttcagatctg atacgttcgg aggtaagtgg tttccctccc
      241 agccagagca tgccattccg tgatttcgat cgacggatcg aatcgattgc atgcagcatc
      301 gctctttctg cagagcgctg gactttctcg attgcgatca cttccttgcg ggactttttc
      361 gctctcatca acgacaccgt cccggtatcc gtcattaccc ctgtcaatgc atccaggtgg
      421 cggccgagag catagggaaa gtctggagga atgagaattg ctccctccac aaggtttgcc
      481 accatccttg ccagggctct ccaccggtct ttctcttctt cgagtatccg gaacagccct
      541 gcgtcagccc gtgtcatgac cctcgtgatc gcttcctgct gggcacgggt atactccatc
      601 tgggaaacga tgatccaccc gcgctccccg gcactcctga catatatgat aggatcggtc
      661 gtagtaaaac gggtgaggta tcgcatatca gcatattcag aggaacctgt catcacatat
      721 gctgccgctc cgctcctttg cagtgcccga tcgagcgcgt ccatactaat ccattagtat
      781 cccggaccac aagtagtttt atcatagaaa ctacctatgt gtaatggacc tggcgacgaa
      841 ggaaaagttc aaatggaaat tttatcgcct tgcggtactg ctgaatatca tcattctgct
      901 cgttgccata ggcatacttg cactgttctg gattcctgaa acataccggc ttccagtggt
      961 tctgctcatt ggtctcctgg ttgttggttt gacgtggtat ttctggggtc aatacaggaa
     1021 aacccgggac tggttggacc acccggtgta gggtgtccat cctggaacaa acggtcctgc
     1081 aatggtaagg gtataatgat cgaaaaagtg aagagtgaaa ttgagctgat gcaacggcat
     1141 atccaagtta tgaggaccgt tgtcgatcat gaaccgatag gtatcatgaa gctctccgag
     1201 atccttgatc tgccgtttca ccggattcgg tactcgctcc gggtgctgga gcagatgggg
     1261 tatatacggg cttcaccttc tggcgctatg gcaactgacc ttgcagcgga tctccttgaa
     1321 cacctcgatg atgagattga tgaaatggtg agacttctcg agggaatgaa aaaagcacac
     1381 tagttgcgtt gtaatggata taacgctact tttaaaagta tttagaaaga atgtaatagg
     1441 gcacagcatt tgtgccgcca taactcagtt ggtagagtgg ctggctgtta accagcatgt
     1501 cacaggttcg agtcctgttg gcggcgcttc atcccgggcc cgtagcttag tccggtaaga
     1561 gcgcccggct cataaccggg cggtcatgcg ttcaaatcgc atcgggccca ttcttcacgg
     1621 tttttcttac gcattgttct gacgtatttc cgattcccgg acaagctgtc atcccttgtt
     1681 tgtttcccga cacacttcct cataaacaca gtgaatccag cgttctatcc ggaatccctc
     1741 acctactgag ggggtgtttt tccggccgaa tatagtcaaa agatatctgt cccgaatgca
     1801 gagggtctac tatgaaatgt cctgtctgtg gcgatgattg tgtaaaagaa gcacatgaga
     1861 tcattcgcat gatccctgca gttttcgcgc ggtgccagga ctgcaggttc aaaatcctcg
     1921 ataaaagtct cccgccccct ccagatgcaa atcttgaggc gtgtcggtgc ggaaggcgtt
     1981 ttatcgatga ggttttcgcc cacctctatg cgcttctggt cagggagggg gtatttaccg
     2041 gaaaggaagc tctcaaggag gtcggatccc ccctgctgca tcccggttat ccccttacca
     2101 cccctccctt tctgccaaag gattcgcttg ttctcctctc ccgacatccc acccgggcag
     2161 tggctcagat gatggtcgat gagatccctg aagtccgggg cgtggtccga tcaggtgatt
     2221 atattcccgg ggttatcgat ccctccctga acagtccgcc ttgcacgtat gacctcctgg
     2281 ccggatgcga tgtgagagcc aacgtgttcc cgacaaaaat gggtcccgtg gtcctctatc
     2341 agcagcagtc aaccattcat atcgagctcc ccaggcggca caacccaaaa ataacctctg
     2401 tcgaggaacg catagcaagg atacgacccg aatggtttgt tgatgcctgc tgcggcgccg
     2461 gcactctcgg gataaccgct gcaaggctcg gcgtccctca
//
LOCUS       DAOOCO010000232         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_42602_length_2498_cov_5.250102, whole genome shotgun sequence.
ACCESSION   DAOOCO010000232 DAOOCO010000000
VERSION     DAOOCO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42602_length_2498_cov_5.250102"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1038
                     /gene="corA"
                     /locus_tag="PK154_10000"
     CDS             70..1038
                     /gene="corA"
                     /locus_tag="PK154_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPW11430.1"
                     /translation="MTVMDYDAAHFTEKTVSQITECFPFRDTETVTWFNVDGLGNTRI
                     IEELGQCFTIHPLILEDILNTDQRPKMEDLDAYIYLNMKMLSFVDPEKDVKVEHVSML
                     LGHNFLISFQEDVGDIFDPVRERIRKDGRIRKFGPDYLAYALIDSIVDNYFVVMEKLE
                     ERVEELEEELVLHPTQASLARISRLKKDMIFLRKSVWPLREVISTLEHSESPLIKETT
                     AIYLRDVYDHTIQVIDTLETLRDMVSGMLDIYLSGLSYRMNEIMKVLTLIATIFIPLT
                     FVAGVYGMNFRYMPELAWEYGYYTVWTVMIGMVVLMLIYFRKKEWI"
     gene            1117..1794
                     /locus_tag="PK154_10005"
     CDS             1117..1794
                     /locus_tag="PK154_10005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPW11431.1"
                     /translation="METGYLFLLAVAPAAFWLWYFYNKDRYEPEPLSWILMVYIFGIV
                     VTIPVAFIEGTLSIIVPEFFIVVLVAPIVEEAGKYLVVKKTVWESLEFDEPVDGIIYA
                     AAAGLGFATLENVIYVFSAVETSLLLALQTGLLRALISVPGHVLFSAMWGYSLGKARF
                     IPQEQRTGVIATGLILAMAFHALFNLLLYDAIGFAVLILVVVPVLWFSVQKKIDQALL
                     SSGYRPR"
     gene            1933..>2498
                     /locus_tag="PK154_10010"
     CDS             1933..>2498
                     /locus_tag="PK154_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HPW11432.1"
                     /translation="MNRSVIPPLSKQRWIVILLLLACLVLSLILRILPWFQMDFPALS
                     VHGDPDVWYNFRQIEVMVSDFPRYNWFDPMTAYPVGKELDWGPLTPFLASLVCLLGGA
                     AQLADIMAVSSWVPVVAGIAMIPVVFFLGRLIAGWKAGIIAAVFIAFVSGEYFYRTMA
                     GIVDHHALEILLSTLFCLFYLWALRKSS"
BASE COUNT          524 a          638 c          661 g          675 t
ORIGIN      
        1 tccaaaaagg ccgggcagcc gccgggaacc ctccatgccg gaggcattcc gtccactgaa
       61 gatgtccgga ttaccgtcat ggattatgat gctgcccatt ttactgagaa gacagtcagc
      121 cagatcaccg agtgttttcc cttccgtgat acggagaccg tgacctggtt caacgtcgat
      181 ggactcggaa ataccaggat tattgaggag cttgggcagt gtttcaccat ccatccgctc
      241 atccttgaag atatcctgaa taccgatcag cggccaaaga tggaggatct tgatgcgtat
      301 atctatctca atatgaaaat gctctcattc gtcgatccag agaaggatgt caaggtcgag
      361 cacgtgagta tgctgctcgg gcataatttt cttatctcgt tccaggagga tgttggggat
      421 atcttcgacc cggtgcgcga acggatcagg aaggatggaa ggatacgcaa attcgggcct
      481 gattacctgg cgtatgccct tatcgacagc atcgtggata actatttcgt ggttatggag
      541 aagctcgagg agcgggtcga agagctcgaa gaagagcttg tgcttcatcc cacgcaggcg
      601 tcactcgcac ggatcagccg gctgaaaaaa gacatgatct tcctgagaaa atcggtatgg
      661 cctctcaggg aggtgatcag cactcttgag cattccgagt caccgctgat caaagagacg
      721 acggccatct acctccgcga tgtgtacgat cacaccatcc aggttatcga tacgcttgag
      781 acattgcggg acatggtctc gggcatgctc gacatctacc tctcggggct ttcctaccgg
      841 atgaacgaga tcatgaaggt gctcaccctg attgccacca tcttcatccc gctcaccttc
      901 gtagcagggg tctatggcat gaatttccgc tacatgccgg aacttgcctg ggaatacggg
      961 tattacacag tctggaccgt gatgataggg atggtggtgc tgatgctcat ttatttcagg
     1021 aaaaaagagt ggatttgagg aaattactca cacatttctc cgttctggca tccaaaccac
     1081 ctattatcat cctttgtgtg aaaggttctg tctggaatgg agacaggcta cctttttctt
     1141 ctcgcagttg cccctgcagc attctggctc tggtattttt acaacaaaga ccggtatgag
     1201 cccgaacccc tttcgtggat cctcatggtg tacatattcg gtatcgttgt caccattcct
     1261 gtggccttca tcgagggtac cttgagcata atcgttcctg aatttttcat cgtggtatta
     1321 gtagcgccga tcgtcgaaga agcgggaaaa tatctggtcg tgaaaaagac ggtgtgggaa
     1381 tccctcgagt ttgatgagcc ggtcgatgga atcatttacg ctgctgcggc cggcctcggt
     1441 tttgccaccc tggagaatgt gatttatgtc ttttctgcgg tcgaaacttc cctgctcctc
     1501 gccctccaga cggggctgct tcgggcactc atctcggtcc ccgggcatgt cctcttctct
     1561 gcaatgtggg ggtattcgct cgggaaggca cggttcattc cccaggagca gcgaacggga
     1621 gtcattgcca ccgggttgat ccttgcaatg gcattccatg ccctgttcaa tctcctctta
     1681 tacgatgcga tcggttttgc cgttctcatt ctggtcgtgg ttccggtgct ctggttctct
     1741 gtgcagaaaa agatcgatca ggcactcctc tcatcagggt atcgcccacg ataagcctgc
     1801 ggtgccctgg tggcagacag gggaattcag agtaataggt atctgttccg tattgaaggc
     1861 gcagaatctg gtggcagatg gccggtccgg gtggggcgaa tctttttcat attcagaaat
     1921 aaggttttat tcgtgaatag atcggtgatc cctcccctct caaaacaacg gtggatcgta
     1981 attctgctcc tcctggcatg tcttgtacta tcccttattc ttcgcattct tccgtggttc
     2041 cagatggatt ttccggctct gtcggttcat ggcgatccgg atgtctggta taacttccgg
     2101 cagatcgagg tgatggtatc agacttcccc cgctataact ggtttgatcc catgactgcg
     2161 tacccggtcg gaaaagagct agactggggg ccgctcactc ccttcctcgc ttcactggtc
     2221 tgtctcctgg gcggagcagc acagctggcc gatatcatgg cggtctcatc ctgggtgccg
     2281 gtcgtcgcgg gcattgcaat gattccggtt gtttttttcc ttggcaggct gattgccgga
     2341 tggaaggccg ggatcatcgc cgccgttttc atagcctttg tatcgggaga gtatttttac
     2401 cggacgatgg ccgggattgt tgatcaccac gctctcgaga tactgctctc aacactcttc
     2461 tgcctttttt acctctgggc tctcaggaaa tcatcaga
//
LOCUS       DAOOCO010000233         2475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_43213_length_2475_cov_4.154959, whole genome shotgun sequence.
ACCESSION   DAOOCO010000233 DAOOCO010000000
VERSION     DAOOCO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43213_length_2475_cov_4.154959"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..795)
                     /locus_tag="PK154_10015"
     CDS             complement(127..795)
                     /locus_tag="PK154_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPW11433.1"
                     /translation="MLDFLLHIDQNLAGLITTFGPGAYLILFLILFLETGCILTVFLP
                     GDSLLFVAGAGAASGLFSLSWLIIIFFLAGVAGDLLNYWLGHHIGLNVFRQRFPDLVK
                     EEYIDRTSLYFERFGKKTIFIGRFIPVVRTFAPFLAGIGTMECRDFLLYNVISAFCWS
                     VAITGAGYILGSIPWIQDHIGLIILITIVFILATIIVIILASAHGLLRAKRGEKNGDD
                     SLLP"
     gene            1003..1341
                     /locus_tag="PK154_10020"
     CDS             1003..1341
                     /locus_tag="PK154_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPW11434.1"
                     /translation="MHEFGIAYDIFKTAQKTARDHHARQVKRVCVDIGEMSMVSPEQV
                     QFLFDTLIEEDPVLRGAVLESTQIPPETRCICGYRGSEKFICPGCGALPELVRGREIV
                     VTHIEIEVDE"
     gene            1342..2337
                     /gene="hypE"
                     /locus_tag="PK154_10025"
     CDS             1342..2337
                     /gene="hypE"
                     /locus_tag="PK154_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPW11435.1"
                     /translation="MKVSLMHGAGGEVMGELLKVLTRIEHNNAGGIGLEALDDGAVIP
                     VDGLNIVFTTDSHVVRPIFFPGGDIGRISICGTINDLAMMGGRPLALSCGMIIEEGFE
                     VADLERIVQSMDAALGESGASLVTGDTKVLEKGALDGIVINTAGIGITGTPVRDSGLL
                     PGDVIIVSGTLGDHGLAIMAERAGLSFGEQIRSDVAPLWGLVSRALEAGTIHAMKDPT
                     RGGFASAINEMARKSRVSIRIDEEQVPLRKSVKSASMMLGIDPLQVANEGKVVMGVPA
                     EEAEAILAAIKSHHYGYDAAIIGRVTEGAHVIMETAVGGERFIEPPIGDPVPRVC"
BASE COUNT          583 a          648 c          755 g          489 t
ORIGIN      
        1 cagtctcgat gactggacag cgaccggcaa cagcgtcggc ggcctctcgg tcacggtgga
       61 atcaaagatc gacaacgaag ggctcatgat ccatcacgcc tgttagggtc cccgccagtc
      121 tccctctcag ggcaggaggg agtcatcccc gttcttttct cctctctttg ccctcaaaag
      181 cccatgggca gacgccagga tgataacgat gatcgtcgcc aggatgaaca caatggtgat
      241 caggatgatc aggccgatat gatcctgtat ccaggggatc gatccaagga tatacccggc
      301 ccccgtgatg gccacagacc agcagaacgc ggatatgacg ttgtagagca ggaaatcgcg
      361 gcactccatg gtgccgatgc cggcaaggaa gggggcaaat gtcctgacca ccgggatgaa
      421 ccgcccaata aagatcgtct tcttcccgaa ccgctcaaaa tacaggctcg ttcgatcgat
      481 gtattcttcc tttacgaggt cagggaaccg ttgcctgaag acgttcagcc cgatatggtg
      541 tccgagccaa tagttcagga ggtcgccagc cacccccgca aggaagaaga tgatgatcaa
      601 ccaggagagg gagaagaggc ctgatgctgc ccctgcacca gccacaaaga ggagcgagtc
      661 cccgggcaga aataccgtaa ggatgcaccc ggtctcgaga aagagaatca ggaagaggat
      721 cagataggca cccggaccaa aggtggttat cagcccggcg aggttctggt caatatgcag
      781 caaaaaatcc agcatgggag tgtaccttcg ccgctactac tctttggaac aggactatat
      841 gaagaattcc ccggcggagg tataccggca cgatacgatc atttattgcg gttccggcag
      901 aactgtacag gaatacggtc ctcctgccgc ctccgcaccc ggacgctccc ggtgcatttc
      961 ccggcacgcg gccggggaaa tcctctttat ctggttgaaa acatgcacga gtttggaatt
     1021 gcatacgata tttttaagac ggcacagaag acagcccgcg atcaccatgc caggcaggtg
     1081 aagagagtgt gcgttgatat cggcgagatg tcgatggtca gtcccgaaca ggtacagttc
     1141 ctgttcgaca ctctcatcga agaagatccg gtcttacgcg gggcagtcct cgagagtacg
     1201 cagatccccc cggagacacg gtgtatctgt gggtaccggg gaagtgaaaa gttcatctgt
     1261 ccgggatgcg gggcgctccc tgagctggtg cggggcaggg agattgtggt cacccatatc
     1321 gagatagagg tggatgagta gatgaaagtc agcctgatgc atggggccgg cggtgaagtg
     1381 atgggggaac tcctcaaggt cctgacgagg atagagcaca acaatgcagg ggggatcggc
     1441 ctcgaggcgc ttgatgacgg ggcagtcatc cccgtagatg ggctgaacat cgtcttcacc
     1501 acggattccc acgtcgtccg gcccatcttc ttcccaggag gggatatcgg gaggatatcc
     1561 atctgcggaa cgatcaatga cctcgccatg atgggtggca ggccgcttgc gctctcctgc
     1621 ggtatgatca tcgaagaggg atttgaagta gcggaccttg agcggatcgt acagtccatg
     1681 gatgccgccc tcggcgaatc cggtgcgagc ctcgtgacag gggatacgaa ggtgctggag
     1741 aagggtgccc tggacgggat agtgatcaat accgcgggta ttggtatcac cggaaccccg
     1801 gtcagggact cggggctcct gccaggtgat gtgatcatag taagcgggac gctcggtgac
     1861 catgggcttg ccatcatggc agaacgggca ggcctgtcat tcggagagca gatccgatcc
     1921 gatgtcgccc cactctgggg cctggtctcc cgtgcccttg aggcagggac cattcatgcc
     1981 atgaaggacc ctacccgggg aggatttgcc agtgcgatca acgagatggc aaggaagagc
     2041 agggtctcaa tccgcatcga tgaggagcag gtgccactgc ggaagagcgt gaagagcgcc
     2101 tcgatgatgc ttgggattga tcccctgcag gttgcaaacg agggaaaagt cgtgatgggg
     2161 gtccccgcag aggaggcaga ggcaattctc gccgcaatca aatcacacca ctacgggtac
     2221 gacgccgcaa tcatcggcag ggtaacggag ggggcccatg tgattatgga gaccgcggtg
     2281 gggggcgaac gcttcatcga acctccgatc ggggatcccg tcccgagggt ctgctgatac
     2341 cggttcatcc tttttccaat gagaatcact tcgtaatttt cataatgtat gcccatatcc
     2401 cgaaatcggg gctcatagga ggtttatatg aaaatgcctc cggtattttc atcttttatg
     2461 cttgtggcct tcaaa
//
LOCUS       DAOOCO010000234         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_43916_length_2449_cov_7.042189, whole genome shotgun sequence.
ACCESSION   DAOOCO010000234 DAOOCO010000000
VERSION     DAOOCO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43916_length_2449_cov_7.042189"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..587
                     /locus_tag="PK154_10030"
     CDS             366..587
                     /locus_tag="PK154_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11436.1"
                     /translation="MRDISRKCSLVNTPTAGTPLVFRAMLKHEKAEGEEVKNLTTLLV
                     KRFDVFQGFPTLRAGGDGMDDTVSGFGVG"
     gene            complement(600..890)
                     /locus_tag="PK154_10035"
     CDS             complement(600..890)
                     /locus_tag="PK154_10035"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HPW11437.1"
                     /translation="MTAEHPYPETGVENRPFSQYTVEDIPDLLAVIGDLKRLITELSA
                     ENARLKAQIAELQSRLNQNSRNSSRPPSMDGFQRPQTPREKGKRPPRGSEGT"
     gene            1034..2449
                     /locus_tag="PK154_10040"
                     /pseudo
     CDS             1034..2449
                     /locus_tag="PK154_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022906.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
BASE COUNT          673 a          602 c          612 g          562 t
ORIGIN      
        1 gccttgcgcc ggaaagactc ctgcattgca tccgctgaga tctcgtaccg gacatcgatg
       61 aagctcttct gctcgcgtga actgccggat gaggttcccg gagaactcct cgtccaccgg
      121 gcatgagaaa tgcacgctgc ccccatatgt tggagagtgt gagtgttctg caggagtttt
      181 cgcatatcgt cttcgacccc ggtcaggttt tgagagcact cgttcacgat tgcaaccacg
      241 gttgccgggc tcacatggtg accaaagagg tcagaaacaa tctcagaggc tcggtcatag
      301 ggaagtaacg ggtagacaca gaggtagacc aggaatgtct tcaggtcgat gccatactgc
      361 acaggatgcg cgacatcagc aggaaatgca gccttgttaa cacacccaca gcggggacac
      421 cgcttgtgtt ccgcgcaatg ctcaagcacg aaaaagcaga gggcgaggaa gtcaaaaatc
      481 tgacgacact ccttgtcaaa cggttcgacg ttttccaggg attccccaca ctgagagcag
      541 gtggagacgg tatggacgat acggtatcag ggttcggggt aggttgaagg gtatgacctt
      601 tatgtccctt ctgacccccg gggagggcgc ttacccttct cccgcggtgt ctggggtcgc
      661 tgaaagccat ccatcgaggg agggcgactg ctgttgcggc tgttctggtt caaccgggat
      721 tgaagttcgg caatctgagc cttcaggcga gcattctcag cactgagttc tgtgatgagg
      781 cgcttgagat caccgatgac tgcaagaaga tccgggatgt cctcaacagt gtactgggag
      841 aagggtcgat tctcaacacc ggtttcagga tatgggtgtt cagcagtcac tatattttgt
      901 tcggacatca ttataagaag atctttcggt ttgggctgaa tcctaacttt gccagttata
      961 acatctgaga gtgttcttag gccaagttag caacgcataa gagtgttctt agtgatgttt
     1021 gaattgagtc aaaatgcaaa cacaactaag aactaatgag attgagccga cggaaaatat
     1081 atcctttcct attggtacca ttttcctcgt tacacggctg tatgacgtcc tgaatttccg
     1141 cactctcttc gggaaacaca agacgcgagg tatcgatatc aatggtttgc ttcaagccct
     1201 gatcagctac aaactgacgg ataacttcag catcaagcga tcccacgact ggatcacccg
     1261 cccggaagtt ctcgctgaat tcaacctccc gcccttcagc gaacgaaccc tctatcgtgt
     1321 ccttgaaact cttggcgcca actgagatga gatcattgcc gacctccagg atgtcctttt
     1381 tgcgcggtat gactttgagc ataccgacat caacatggac tggaccagca tcgtccttca
     1441 cggcactgag gcgaagctcg gaaagtatgg ctacagccgg gatcaccggc cggataagaa
     1501 acagatcacc gttggcgtca cagaacttgc cgacccgatc aatgtcccga ttgggatgac
     1561 aatcgagcct ggcaatctca atgaccagac gcacttcaag aagacctatc ggcagtcacg
     1621 cgaccggctc agagagggtt ctctcgtgat attcgataaa ggagcgaata gtgtcgccaa
     1681 tacccagatg atccgggcag atcatctgca gtacatcacc ggaaagaaac tcaacaagag
     1741 cgatgacaag attatcgctg catttgaaac ctatcatcct caagttgttg atgcagaatc
     1801 cgggattcgc gggattatca tcgagaaacc aaacagcacc aactatctct atttctccaa
     1861 aaagctgcaa aaagagcaac tggaatccag agccagaaaa gttgtgcgag agatacagga
     1921 agcaaaggcc attcaggaaa gcattgacaa gaagaagagt cttccaaaac gattccggat
     1981 aaacaatctg ctggtggatg tggactactc catccagaca aaacttctcg aactctcgga
     2041 agaggatgcc gtcaagctcc ttgaagagaa gttcatcacc ggtcgagaag gatttttctg
     2101 cctgaaatca agcaagaagt tgacacttac cgaagccctg ctgacctacc gcaaaaagga
     2161 ctcgattgag aagatattca actcgctgaa gaatgagacc gagatcaagc cgttgcgtgt
     2221 atggacggat aacagcattt acggtgcatt aatcatcgga ttcattgcac aactctttgt
     2281 ctccctgatc cgattcgaga tcccggaact gaagcatacg tcaacgaaat tcatcaaaca
     2341 ggcgctattg aatttgacag ttaccattga ttcgtggatg aggaagacaa aaaagtacat
     2401 tcactccaat tttgatgcca ttaacacgat gattctacgg cataattgg
//
LOCUS       DAOOCO010000235         2441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_44151_length_2441_cov_5.712490, whole genome shotgun sequence.
ACCESSION   DAOOCO010000235 DAOOCO010000000
VERSION     DAOOCO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44151_length_2441_cov_5.712490"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK154_10045"
     CDS             <1..224
                     /locus_tag="PK154_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HPW11438.1"
                     /translation="GALDALFGGFVGLLIGVGIFARVYPSLHRTILTYGRIKKETVHE
                     LLGLPEWLVVIGGCIGIVAFLYLLEVAGF"
     gene            complement(257..2035)
                     /locus_tag="PK154_10050"
     CDS             complement(257..2035)
                     /locus_tag="PK154_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HPW11439.1"
                     /translation="MRIAVVHRDRCHSRKCGTECILYCPRVRTGDETVIIGEDKKAVI
                     SEELCVGCGICVKKCPFEAIDIVSLPEELEHATHRYGKNGFALYGLPIPVEGKVTGIL
                     GANGIGKSTAVKILSGQLKPNLGEFEREAGWEEILRKYTGTELFDYLRTVSEKRLKVA
                     IKPQYIDYIPRVFSGTPAELLKTTDERGKLDEYIGALMLTPLLDHDIATLSGGELQRV
                     AIVACLAREADFYFLDEITPFLDIYQRMAAARIIRELAATRPIVIVEHDLAILDMLAD
                     TVHVAYGKPAVFGVITQPKGVRVGINEYLEGYLPEENVRFREYPVVFEKRAHEKHAER
                     ALLFEFPAMSKSYDRFRLEISPGEIRTGEVLGVVGANGIGKSTFARLLAGVEEPDEGP
                     LSGETRISYKPQYLKATSSDTVEFSLRQITRSFDSSAYQHDIIEPLSLSPLLQSPIDT
                     LSGGELQRVAIAACLSRDADLYVLDEPSAHLDVEQRVRITRMFKQHVDGKDAAILVID
                     HDIYLIDMISERILFFDGEPGVRGEATGPFDMRQGMNRFLSRLGVTFRRDKTGRPRIN
                     KPGSYLDRDQKSSGEYYYYTDKEAGE"
     gene            complement(2043..2405)
                     /locus_tag="PK154_10055"
     CDS             complement(2043..2405)
                     /locus_tag="PK154_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11440.1"
                     /translation="MSADEVKEKEELPKKLSPEEKRQENSRKIKRTLIAAALGIITGV
                     ISYLAIDSSQILGFQSYTILALLIMIAGIVVQKHIFMLMHLFLKPLGFKDWFYQGFMT
                     FAFWFITWTILLTSGIIA"
BASE COUNT          489 a          696 c          686 g          570 t
ORIGIN      
        1 agggtgccct cgatgccctg tttggtggat tcgtcggcct gctcatcggg gtcgggatct
       61 ttgcgagggt atatccctct cttcaccgga cgatcctcac ctacgggagg ataaaaaagg
      121 agaccgttca cgaactttta gggctgccgg aatggctggt ggtcattggg ggatgcatcg
      181 ggatcgtcgc tttcctctac ctcctcgagg tcgcaggttt ctgaccggca ttcccgcgtt
      241 ttttttacct ggccgcctat tcccccgcct ctttatcggt ataatagtaa tattcaccgc
      301 tcgatttctg gtcccggtcg aggtatgaac caggcttgtt gatcctcggc ctcccggtct
      361 tgtccctcct gaaggtaacc ccaaggcggg agaggaagcg gttcattccc tgccgcatat
      421 cgaaaggacc agtggcctca cccctcaccc ctggttcccc atcgaagaag aggatccgct
      481 cgctgatcat gtcgatcagg tagatatcat ggtcgatgac aaggatggca gcatccttcc
      541 catcgacatg ctgcttgaac atcctggtga tccggacccg ctgctctaca tcgaggtggg
      601 cgctcggctc atcgagcacg tagagatcgg catcccttga aaggcaggca gcgatcgcga
      661 cccgctggag ctctccccct gagagggtat cgatcgggga ctggaggagc ggagagagcg
      721 agaggggttc gatgatatcg tgctggtagg ctgagctgtc aaacgaacgc gtgatctgcc
      781 tgagggaaaa ttcaacggtg tctgatgacg tggctttgag atactgaggt ttataggaga
      841 tccgggtctc tccggaaagt gggccctcat caggctcttc cactcccgcc aggagccggg
      901 caaaggtact ctttccaatc ccgttcgcac cgaccacccc gagcacttcc cccgtgcgta
      961 tctccccggg ggatatctca agccggaacc ggtcgtagct cttgctcatt gccgggaact
     1021 cgaaaaggag tgcacgttcg gcatgcttct cgtgtgcacg cttctcgaac accaccgggt
     1081 actccctgaa ccgcacattc tcttcaggga ggtagccctc caggtactca ttgatcccga
     1141 cacgcacccc tttcggctgg gtgataaccc caaaaacggc cggttttccg taggcgacat
     1201 ggacggtatc cgcaagcatg tcgaggatcg caagatcgtg ctcgacgatc acgatcggcc
     1261 gggtggcagc aagttcccgg atgatccggg cggcagccat ccgctggtag atgtccagga
     1321 acggggtgat ctcatcgagg aagtaaaagt cagcctcgcg ggcaaggcag gccacgattg
     1381 ccacacgctg gagctctccc ccggaaaggg ttgcgatgtc atgatcaagc aggggagtga
     1441 gcatcagggc cccgatgtac tcatcgagtt ttcccctctc gtcggtagtt ttgaggagct
     1501 ctgcaggggt tcccgaaaac acccgcggga tataatcgat atactggggt ttgatcgcca
     1561 ctttcagccg tttctctgag accgttctca ggtagtcgaa gagctcggtg ccggtatatt
     1621 tcctgagaat ctcttcccac ccggcttccc gttcgaactc gccaaggttc ggcttgagct
     1681 ggcccgaaag gatcttgacc gcggtactct tcccgatacc atttgcgccg aggatgccgg
     1741 tcacctttcc ctcgaccggg atgggaaggc cgtacagggc gaacccattc ttcccatacc
     1801 ggtgggtggc atgctccagc tcctcgggga ggctgacgat gtcgatcgct tcgaacgggc
     1861 atttctttac gcagatcccg cagccgacac agagctcttc cgagatgacc gccttcttgt
     1921 cctcgccgat gatgaccgtc tcgtctccgg tcctcacccg cggacagtac aggatgcact
     1981 cggtgccgca tttcctggaa tggcaccggt cacggtggac tactgcgata cgcatgggtg
     2041 gttcacgcta taattccgct cgtcaggagg atggtccagg tgatgaacca gaaggcaaag
     2101 gtcatgaaac cctgatagaa ccagtccttg aacccgaggg gctttaaaaa gaggtgcatc
     2161 agcatgaaga tatgtttctg caccacgatg cctgcgatca tgatgaggag ggcaaggatg
     2221 gtatagctct ggaacccaag gatctgtgat gagtcgatgg caagatacga gatcaccccg
     2281 gtgatgatcc ccaaagccgc tgcaataagg gtacgtttga ttttcctgct gttttcctgc
     2341 cgcttctctt cgggtgagag tttctttggc agttcttcct tctcctttac ctcatctgca
     2401 ctcatcgtga taccagtagt cctatttttt agtctttaag c
//
LOCUS       DAOOCO010000236         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_45399_length_2397_cov_5.587105, whole genome shotgun sequence.
ACCESSION   DAOOCO010000236 DAOOCO010000000
VERSION     DAOOCO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45399_length_2397_cov_5.587105"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PK154_10060"
     CDS             <1..847
                     /locus_tag="PK154_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPW11441.1"
                     /translation="RCVDCLLSRVMLECKLCGASDPRSEEVVAACADLLASLRHQPLL
                     HPQIASAMHRKAYDMLGNDDPFLSLKEESDRIAREVCSAVRGRLSGFRDYVLASIIGN
                     TFDYGVKGHQVTGDFLGYFEQEYAAGLAIDHTDRIARLIDRVVYFTDNCGEIVFDRLL
                     LEYLHRQGSHITLAVRDMPILNDATMAEAHALHLERYADVLTSTGCGCEIGVRLDCMP
                     PELADAVDDCTLIIAKGMANYESISEFTGMPPVAFLMAAKCDPIAEEVGVVRGAKVAM
                     LRDRN"
     gene            1044..>2397
                     /locus_tag="PK154_10065"
     CDS             1044..>2397
                     /locus_tag="PK154_10065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW11442.1"
                     /translation="METNHWFFILIIGIGVVLTAGCTQPVTETQRSPETPATTMTVPI
                     QPLTGSDAVTSQRIDNYLTALTDAGRFSGTVFVARGDTVLLSKGYGMANHEFSVPNTP
                     QTVMPIASNTKQLTAAAIMKLQEQGRLNVTDPVTQYIPGVPGWKDIRIYQLLNHTAGI
                     QSDGAFSLTDRTDLTLPETMTRITGLPLTFEPGTSYGYSNNGYITLSYLIEKASGMSY
                     EEYVKTNLFLPLGMNRTGQDNARDVFSHRASGYTTFDGNYIHYDLQNIHNTWGAGSIH
                     STTEDLFRWVHAFHTSEIILSRQSKTTMIENHYGIDSGKIMNHTYIGHTGRNFCFISQ
                     TLYFPEEQVSIIFLTNYDRTPVVTLVNDLPAIVFNEPYSLPQKIDQEAVPMTAAALAE
                     YTGTYAPAWERSWEITLYSEGDRLFYDSVMPGDRNVELFYMGNDTFFVTPESNDTFIF
                     T"
BASE COUNT          555 a          706 c          622 g          514 t
ORIGIN      
        1 ccgttgtgtt gactgcctcc tctcccgggt catgctcgaa tgcaaactct gcggggcatc
       61 cgatccccgt tcagaggaag tggttgcggc atgcgccgat ctccttgcat cgctccgaca
      121 ccagccgctc ctccaccccc agatcgcgag cgccatgcac cggaaggcat acgacatgct
      181 tgggaacgat gatccgttcc tctcccttaa agaggagagc gaccggatcg cccgggaggt
      241 ctgctccgcg gtgcggggcc ggctctcggg gttccgcgat tacgtgcttg caagcatcat
      301 cgggaacacc ttcgattacg gggtcaaggg ccaccaggtg accggcgatt tcctcgggta
      361 cttcgagcag gagtatgccg ccgggcttgc catcgaccac accgatcgga tcgcccgcct
      421 gatcgaccgg gtggtctact tcaccgacaa ctgcggggag atcgtctttg accggctcct
      481 cctcgagtac ctccaccggc agggatccca tatcaccctc gccgtccggg acatgccgat
      541 cctgaacgat gccaccatgg ctgaggccca tgccctccac ctcgaacgat atgccgatgt
      601 cctcaccagt acggggtgcg ggtgcgagat cggggtccgc ctcgattgca tgccgccgga
      661 actcgcagac gcggtcgatg actgcaccct gatcatcgcc aaggggatgg caaactacga
      721 gtcgatctcc gagttcaccg ggatgcctcc cgtcgccttc ctgatggccg ccaagtgcga
      781 cccgatcgct gaagaggtcg gcgtcgtgcg gggagccaag gtggccatgc tccgtgaccg
      841 gaactagctg gtatccctga tcggacgatt tttcagtact tcctgaatcc tgcatacttg
      901 aatacagggt caaaatgttg aaaagacgtt atatcattgc gaaaaggtaa acagagcctt
      961 catccatgat tcaaggcagt ttgtgcaaat aacgaaaatg tagcgtttat ctttaactga
     1021 atttacgatc aaatgtaatc ccaatggaaa cgaatcactg gttcttcatc ctcatcatcg
     1081 gaatcggggt agtccttact gcgggatgca cccagcctgt gactgaaaca cagcgatctc
     1141 cggaaacacc tgccacgacc atgacggttc cgattcaacc cctgaccggc tctgatgcgg
     1201 tcacctccca gaggattgac aattatttga cagcacttac cgatgccgga aggttcagcg
     1261 ggacggtgtt tgtggcacgg ggcgataccg tactcctctc gaagggatac ggcatggcaa
     1321 accacgagtt ttcggttcca aacacgccgc aaacagtaat gccaatcgct tcgaatacca
     1381 agcagcttac cgcggccgcg atcatgaaac tgcaggaaca gggacggctc aatgtcaccg
     1441 acccggtcac gcagtatatt cccggtgtcc cgggttggaa agatatccgg atctaccagc
     1501 tcctgaacca caccgccggg atccagtcag acggggcatt ctcgctcacc gaccggaccg
     1561 atctcaccct ccccgagaca atgacgcgga taacaggcct gcctctcacc tttgaaccgg
     1621 gaaccagcta tggatattcc aacaacggtt atatcacgct gagttacctc atcgagaaag
     1681 cgtcggggat gtcctacgag gaatacgtga aaacgaacct gttcctcccc ctcggtatga
     1741 accgcaccgg gcaggacaat gcgagggatg tcttctccca ccgtgcctcg ggctatacca
     1801 ccttcgatgg gaactatatc cactatgatc tgcagaatat ccacaacacc tggggcgccg
     1861 ggtccatcca ttctacgact gaagacctgt tccggtgggt acatgccttt catacctcgg
     1921 aaataattct ttccaggcag tcaaagacaa cgatgataga gaaccactac ggcattgaca
     1981 gcgggaaaat aatgaatcat acctacatcg gtcataccgg gcgaaacttt tgttttatct
     2041 cgcagacgct gtatttccct gaggaacagg tgagtatcat attcctgaca aactatgaca
     2101 ggactccggt ggtcactctg gtgaacgatc tcccggcgat tgtctttaac gaaccatatt
     2161 cactgccgca gaagatcgat caggaggccg tccccatgac agcagcggca ctcgccgaat
     2221 atacggggac gtatgcaccg gcatgggaga gatcgtggga gatcaccttg tattcagagg
     2281 gagaccgcct tttttacgat tcggtcatgc ctggtgacag gaacgtcgaa ctcttttata
     2341 tgggcaatga tacttttttt gtaacgccag agtcgaacga taccttcatc ttcaccc
//
LOCUS       DAOOCO010000237         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_45653_length_2388_cov_3.975139, whole genome shotgun sequence.
ACCESSION   DAOOCO010000237 DAOOCO010000000
VERSION     DAOOCO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45653_length_2388_cov_3.975139"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..439)
                     /locus_tag="PK154_10070"
     CDS             complement(11..439)
                     /locus_tag="PK154_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW11443.1"
                     /translation="MEQGHPSETAGVPSREKCIDPYLRCAFNGAAQTALGVSGSALLA
                     HSPQGCEYLVNNAFASQECDYMETVTLCTKLCVDEIVHGGEELLARTIREARDLPISV
                     LFVISACGPEIVGDDIRAVCETLQPEVKFRLVPIQSPGFR"
     gene            complement(417..1841)
                     /locus_tag="PK154_10075"
     CDS             complement(417..1841)
                     /locus_tag="PK154_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HPW11444.1"
                     /translation="MENDAIVFHGTLQQLLGKVESGEMIPKLQASHAPMCKFFTAYYV
                     ISGIRHVVPLVHGPTGCPLYMSDFVRTRECCEIRGVPLEPTACTALDESHVIYGGEGK
                     LLEAVKEAYVKYHPDLIVILSCCCSGIIGDDVESIAREAEKLVGCRVLALKSEGFGGD
                     FRSGYEDAFKLIMELMDPPTTSMKGTINILGARWGPSPTEFSWDMDEIERLLALIGVK
                     INAIIAGGCTVEQIRHAREVELNASWCYDWGQKLGDLMQERFGIPYSRTGQPYGLEAT
                     KEWLLGVATPLGMEEQALAVIEKETKEVAPEIAYLEQALSGKTAIIEISEFPGPIRAL
                     SLARMAAEFGAHPVVINVHPYTIKERMPSIKFLLERGVNPEVILTQGLFRLGSFRSSK
                     ETELELEAIVRQYDKPLFFGNSGRFPPCPVVNLTLMNPMFQPQYGFRGIKNIAALVRQ
                     ALEHKDLPRSRLFRGMLYGTGTPQ"
BASE COUNT          513 a          626 c          679 g          570 t
ORIGIN      
        1 cgtactgtga tcaccggaag ccaggggact ggatcgggac gagccggaac ttcacctccg
       61 gctggagggt ctcgcagacg gcacggatgt cgtcaccgac aatctcgggg ccgcaggcac
      121 tgatcacgaa caggaccgag atagggaggt cgcgtgcttc ccggatggtg cgtgcaagca
      181 actcctcgcc accgtgcacg atctcgtcca cgcagagttt cgtgcagagg gtgacggtct
      241 ccatatagtc gcactcctgc gaggcaaacg cgttgttgac gagatattcg cacccctggg
      301 gtgaatgggc gagaagggcg gagcccgata cgccaagggc agtctgtgct gccccgttga
      361 acgcacagcg gaggtaggga tcgatgcatt tctcgcggga tggtacgccc gcggtctcac
      421 tggggtgtcc ctgttccata cagcatcccc ctgaaaaggc gggaccgggg aaggtccttg
      481 tgttcgagtg cctgtcgcac cagagcggcg atgttcttga tcccccggaa gccgtactga
      541 ggctggaaca tcgggttcat gagggtcagg ttcacgaccg ggcagggggg gaagcggccg
      601 gagttgccaa agaagagggg tttgtcatac tgcctgacga ttgcctcaag ctccaattcg
      661 gtctcttttg atgagcggaa actccccagc cggaagaggc cttgtgtcag gatcacctcc
      721 gggttgaccc ccctctccag caggaacttg atgctcggca tccgctcctt tatcgtgtag
      781 gggtgcacat tgatgacgac gggatgtgct ccaaactccg ctgccatccg tgcaagggag
      841 agggcccgga tcggcccggg gaattctgaa atctcgatga tggcagtttt ccctgatagt
      901 gcctgttcaa ggtaggcgat ctccggtgca acctcttttg tctccttctc gatcacggca
      961 agggcttgtt cctccatccc aaggggggtt gcaacgccga gcagccattc ttttgttgcc
     1021 tccagcccgt acggctgccc ggtccggcta tacgggatgc cgaaccgttc ctgcatcagg
     1081 tcacccagtt tctgccccca gtcgtagcac catgaagcat tcagttcgac ctcgcgggca
     1141 tgcctgatct gctcgaccgt gcagcctccc gcgatgatag cgtttatttt tactccgata
     1201 agggcaagga gccgttctat ctcatccatg tcccaggaga actccgttgg tgaagggccc
     1261 cagcgggcac cgaggatgtt gatggtccct ttcatgctcg tagtaggggg gtccatcagc
     1321 tccatgatca gtttgaacgc atcctcgtac ccgctccgga agtcacctcc gaatccttcg
     1381 cttttaagcg cgagcacccg gcaaccgacc agcttctcgg cttcgcgggc gatactctcg
     1441 acatcatccc cgatgatccc ggaacagcag caggagagga tgacgatcag gtcaggatgg
     1501 tactttacat aggcttcctt caccgcttca aggagttttc cctcccctcc gtagatgaca
     1561 tggctttcat caagggcggt gcaggcggta ggttcgagcg ggaccccgcg gatctcgcag
     1621 cattccctgg tcctgacaaa atcggacatg tagagggggc accccgtggg gccatggacg
     1681 agggggacga catggcggat accactgata acgtagtagg cggtgaaaaa tttgcacata
     1741 ggggcgtgcg aggcctgtaa cttcgggatc atctcgccgg attccacttt tcccagcagt
     1801 tgttgcaggg ttccatggaa gacgatagca tcattctcca tgactggctc actttaccat
     1861 ctcgcatgga tgggtatata gttagggata gggatccccc tccttatcag gcctggtctt
     1921 ccggtgtttc gatacggaac aacaggggga gaagatatga acgtctgagt cttaatgatt
     1981 gcacatgacg ggactacgtc tctcgttacc ttccccgatt ggaaaatgca cgtcaatgga
     2041 ggggataaaa tccaactata tgatttcatt ccggagcagg aattgtcata ctggggttca
     2101 ttgaaatttt cgcacggttc ttttccgata gggtaaccgt gggttttcga tttttcgaag
     2161 ggggaggtat cagaaacacg tgtaataatt attttaaaat tgttatatta tatcataata
     2221 attctttaag tattatagac aatgtttaca aattattatg aaatcattta atagatgcta
     2281 cgattaagaa taaaagtatg aggtttatac gaccactctc tctcgtgact tgttgtatcg
     2341 tcattgcagg gcttctttca gctggatgtg tgtcgccgaa tgataagg
//
LOCUS       DAOOCO010000238         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_46334_length_2366_cov_3.555171, whole genome shotgun sequence.
ACCESSION   DAOOCO010000238 DAOOCO010000000
VERSION     DAOOCO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46334_length_2366_cov_3.555171"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PK154_10080"
     CDS             <1..1029
                     /locus_tag="PK154_10080"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPW11445.1"
                     /translation="PVAIEYPYGGFSSGASASSGQSFVSTTGTLWTDLTSVYKNSNVC
                     LKAFTTKGPDSSVQVPTSTPTPQPSSGGIDTRSPSVSITSPRAYSTIAPGSSVQVAWT
                     AIDNVAVAGVDIGYSKDKGKTWVTVARNLVTSGTYSLGVPSDISGTFIIRVTARDPAG
                     NEGSSTKSCMVKSSSQMKGSILTTALGPVDTVTKSVSSSPVLAMDTSIPTIAISSPPP
                     YRSIMPGTTLQVIWHAADTAGIAGVDIWISKDYGRSWETVAKNLAPSGTYPLNVPADV
                     SGTFLIRAVAWDNAGNAGSSIRNCIVSTSSPHAADQGMEGSAALIPPLSPLSDFQPYP
                     RLAEMQYR"
     gene            1239..2225
                     /locus_tag="PK154_10085"
     CDS             1239..2225
                     /locus_tag="PK154_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW11446.1"
                     /translation="METFPASGCRFCHEGAKMVLFITGVCHRSCWYCPLSRERKGKDQ
                     VYANEHCISSPDEAIAEARNMRAKGTGITGGEPLGVPDRVLSYARRLKEEFGPGHHIH
                     LYTSHAPSEKELSELLGVIDEIRMHPPVSCWKEIGTSGYLRSAELASAMGFETGFEVP
                     ALPEIDLLIPALPHLHFLNINELEWGETNAHEMRRKHLALEDSIHNAVEGSRAWCRNI
                     VKNENVYFCSSAFKDSVQLRNRLIRTAETTARPFDEISDEGTIIYGRIEGALSVPEAV
                     RELGTGMYEEREGALETAWWILFERGDEIEGRKSIIERYPNRGIIVEVTPLR"
BASE COUNT          567 a          647 c          643 g          509 t
ORIGIN      
        1 cctgttgcaa ttgagtatcc ctacgggggc ttctcttcgg gggcaagcgc ctcttccggc
       61 cagagttttg tcagtacgac cggaacgctc tggaccgatc tcacctccgt atacaagaat
      121 tcaaacgtct gcctgaaggc atttaccacc aaaggtccgg attcgtcagt ccaggtaccg
      181 acgtccaccc cgactcccca gccatcatca ggtgggatag acacgaggtc gccatcagta
      241 tccatcacct ctccccgggc atacagtaca attgctcctg gttcctccgt ccaggtggca
      301 tggactgcaa tcgacaacgt ggcagttgcc ggagttgata tcgggtactc caaggataaa
      361 ggaaagacat gggtgacggt tgccaggaac cttgtcacat cagggactta ttccctgggt
      421 gttccctcgg atatatcagg gacattcatc atccgtgtga cggcgcggga tcctgcagga
      481 aacgagggat cttcaacaaa aagctgtatg gtaaaatcat cctctcaaat gaaaggatcc
      541 atacttacta ctgctcttgg cccggtcgat acggttacca agagcgtgtc ctcatctcct
      601 gtccttgcga tggacactag tattccgaca atcgcgatct catcgccacc cccttaccgc
      661 tcaataatgc ccggaactac cctccaggtg atctggcatg cagccgacac ggccggtatt
      721 gccggggttg atatctggat ctcaaaagat tacggccgct catgggagac ggtggcgaag
      781 aaccttgccc cctcagggac gtatcctttg aatgttcctg ctgatgtttc cggtaccttc
      841 ctgattcgtg cggtggcatg ggacaatgcc gggaacgcgg gatcctcgat aaggaactgt
      901 atcgtgagca cctcctctcc tcatgcggct gatcaaggga tggaaggatc agctgccctg
      961 atcccacccc tgtctcctct ttcagatttc cagccatatc cacgccttgc agagatgcaa
     1021 taccgttaaa ttcgtgagag cctcctttct tctttcctct gttccctcac ctggggatcc
     1081 cctgaaaatt tgacactcca gcgaccatca acccatgagg cacaatcagc gggggagaag
     1141 gggcctgaaa gacgggaggc aacgaggtat ctgacaggcc accaggttcc ggcagtgaaa
     1201 ggttcaaact gaacaacgac caatgatatt cctgacgaat ggaaacgttc ccagcttccg
     1261 ggtgcaggtt ctgccacgaa ggcgcaaaga tggtcctctt catcacggga gtatgccatc
     1321 gctcctgctg gtactgcccg ctctcccgtg aaagaaaagg aaaggatcag gtgtatgcca
     1381 atgaacactg tatctccagc cctgacgaag caatcgccga agcaaggaat atgcgtgcaa
     1441 agggtaccgg catcaccggg ggagaacccc tcggggttcc tgaccgggta ctcagctatg
     1501 ccagacgatt gaaagaagag tttgggccag ggcaccatat ccacctgtat acatcccatg
     1561 caccctcaga aaaggaatta tcggagctcc tgggtgtgat cgatgagatc cggatgcatc
     1621 ctcctgtcag ctgctggaag gagattggaa caagtggata cctcaggtca gcggagctgg
     1681 catctgccat gggttttgag acaggattcg aagtgcctgc gctgcctgaa attgacctgc
     1741 tgatcccggc tcttccccat ctccacttcc tgaacatcaa tgagctggaa tggggagaga
     1801 ccaacgctca tgagatgcgg aggaaacacc tcgcccttga ggattcgatc cataacgcgg
     1861 ttgagggatc ccgcgcctgg tgccggaaca tcgtaaagaa tgaaaatgtc tatttctgca
     1921 gctctgcctt caaggactcg gttcagctca ggaaccggct catcaggaca gcagagacca
     1981 cggctcgccc tttcgatgag atcagtgacg aggggacgat catctacggg aggattgaag
     2041 gtgccctttc agttccggag gccgtccgtg aactcgggac tggaatgtat gaagagcggg
     2101 agggagcact tgagacagcc tggtggattc tgttcgaacg aggggacgag atagaaggga
     2161 gaaagagcat tatcgagcgg tacccgaacc gcggtattat agtcgaggtg acccccctca
     2221 gatgatcagg gatctcctcg aatcggtgta tgaacgatcc ctgatggccc agatacggca
     2281 tataccccac catgtggcaa tcatccagga cggaaacagg aggtttgcga catcgcgcgg
     2341 acttatccgg agggaagggc atcgtg
//
LOCUS       DAOOCO010000239         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_46865_length_2348_cov_5.188399, whole genome shotgun sequence.
ACCESSION   DAOOCO010000239 DAOOCO010000000
VERSION     DAOOCO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46865_length_2348_cov_5.188399"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK154_10090"
     CDS             complement(<1..573)
                     /locus_tag="PK154_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HPW11447.1"
                     /translation="MRIAEGASSELSPELLERIEVFQKGEITEYEIYQKLAALIPDPH
                     NRMVLEKMAREELGHYSFWKRFSGKDMGPDRLKLVFYYLVTRIFGLTFGIKLMEKRES
                     EAVETYREILVVIPETARILEDEKEHEHQIVGMLDEKSLRYVGSVVLGINDALVEFTG
                     SLAGFTLALQNTQLIAAAGLIMGIAASLSMG"
     gene            complement(732..1841)
                     /locus_tag="PK154_10095"
     CDS             complement(732..1841)
                     /locus_tag="PK154_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW11448.1"
                     /translation="MRGVIKRRREFLHLMRVYTVEKGSFTVTDVQNGAGVPRSTAQDW
                     ITRLVDEGCVIQREKKQGRSPARYATISALPQSACRKIFTTVDGDQVQIFHECLSGGC
                     AAFCGHHHMLANGVVKQVRRDGQLLREFARIGYSDVKIGLPPSPAVGVVGVTRDGDDI
                     IQHIRCIGGPAYSLTDMMGHADGVCDILVRREGTIVVGAVRTRALSHLIIGIDDTDSS
                     EGGATFALALALLQYLGARKGVIPIDHRVVMLSPDIPWRTAGNSCSYIELATTHELIG
                     KVVERAILFVEDEALSGEWGIAVKQGFCIDPPLRLYGKKAREEMVGREFAEETAGKQG
                     ITLHGGRGVIGALAAVALSGLPNEILLSPEQNIPP"
     gene            complement(1825..>2348)
                     /locus_tag="PK154_10100"
     CDS             complement(1825..>2348)
                     /locus_tag="PK154_10100"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038836.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPW11449.1"
                     /translation="RVLAVYGILNGTSNFILTRMAAEGLTYEQALLEAREMGYAEADP
                     TYDVKGIDAAIKLVILSNTIWKRNVKLSDVTITGIDMLTNDALRLAEEQHCTIRLIAE
                     AIPEKGILRVAPRILPRTHPLVVENTLNAITIDTDMAGEITLIGKGAGSRETASACIG
                     DLLFIKDSHARGN"
BASE COUNT          537 a          664 c          560 g          587 t
ORIGIN      
        1 ccccatcgag agggatgcgg cgatacccat gatcaatcct gctgccgcaa tcagctgggt
       61 attctggagg gcaagggtaa accccgcgag gcttccggta aattcaacaa gcgcatcatt
      121 gataccgaga accaccgatc ccacgtatcg gagactcttt tcatcgagca tgccgacgat
      181 ctgatgttca tgttcctttt catcctccaa tatccgggct gtttccggga tgaccacaag
      241 gatttcacgg tatgtctcca ctgcctcgga ttcccgtttt tccatcagtt ttataccaaa
      301 tgtcaggccg aagatacggg taacgaggta atagaacacc agtttcagcc tatcaggtcc
      361 catatctttt ccactaaacc gtttccagaa tgaataatgg ccgagctcct ctctcgccat
      421 cttctcaagg accatcctgt tgtggggatc cgggataagt gctgcaagct tctggtaaat
      481 ctcatattca gtaatctctc ctttctggaa cacctcgata cgctcaagaa gttcagggga
      541 aagctcactg cttgcgccct ctgcgatacg cataggatga tatcagcacg agcattgttc
      601 aatgttaccg caatacatcg aaattgacct gcgtgttcca aatggaagcg tgaaatgaga
      661 tttgtaacag tagggtggtg caggtaaact caatccggta tcgaaaatga atccattgag
      721 atcatgaggt gttaaggagg aatattttgt tccggtgaga ggagaatctc attggggagc
      781 ccggaaagtg caaccgcagc aagtgccccg ataacgcctc tgcctccatg caaggtaatt
      841 ccctgctttc cggccgtctc ctctgcaaat tccctgccaa ccatctcttc ccgtgccttt
      901 ttgccgtata atctcagagg gggatcgata cagaatccct gtttcacagc aattccccat
      961 tcccctgaaa gagcctcgtc ctcaacgaac aatattgccc gctccaccac ctttccaatt
     1021 aattcatggg tcgttgcaag ttcgatgtag ctgcatgaat ttccggctgt cctccacggt
     1081 atatccgggc tgagcatgac gacacggtga tcaatgggga tcactccctt tctcgcacca
     1141 agatactgga gcagggcaag cgcaagggca aaggttgccc ctccctccga actgtcagtg
     1201 tcatcgatgc caattatcag gtgtgaaagg gcgcgggtcc tgaccgcacc aaccacaatg
     1261 gtaccttccc ttctcaccag gatatcgcat actccgtctg catgccccat catgtcggtc
     1321 aaagaatatg cagggccccc gatacagcgg atatgctgta tgatatcatc tccatcacgg
     1381 gtgactccga ccacacccac tgcaggggaa ggaggcagcc cgatcttcac atcagaatat
     1441 ccaatccgcg cgaattcacg gaggagctgc ccgtccctcc gcacttgctt caccacacca
     1501 ttggcaagca tatgatgatg cccgcagaag gcggcacatc ccccgctcag gcattcgtga
     1561 aagatctgaa cctgatctcc atccacagtg gtaaaaattt tcctgcaggc gctctggggt
     1621 agggctgata tggtagcgta tcgtgcaggt gaacgcccct gttttttctc gcgctggatg
     1681 acacacccct cgtcaaccaa acgggttatc cagtcctggg ccgtactccg gggaacccct
     1741 gccccgttct ggacatcggt gacggtaaat gacccctttt cgacggtata cactctcatc
     1801 agatggagaa attcccgtct ccgtttaatt acccctcgca tggctatcct tgataaagag
     1861 gaggtcgcca atgcaggcac tggccgtctc cctcgaacct gccccctttc cgatgagggt
     1921 gatctctcct gccatatccg tgtcaatcgt tattgcattg agggtgttct ctaccacgag
     1981 aggatgagtt cgcggcagga ttcgcggagc gactctgaga attccttttt ccggtatggc
     2041 ctctgcaatc agccggattg tacaatgctg ctcctctgcc agccggagcg catcgttcgt
     2101 gagcatgtct atcccggtga tggtgacgtc agagagtttc acgtttctct tccatatggt
     2161 gtttgaaagg attacgagct tgatggctgc atcgattccc ttcacatcat aggtaggatc
     2221 tgcttcagcg tatcccatct cccgggcttc caggagcgcc tgttcatagg tgagaccttc
     2281 tgcggccatt cttgtcagga tgaaatttga ggttccgttc agtatgccat atacggcaag
     2341 cacccggt
//
LOCUS       DAOOCO010000240         2338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_47181_length_2338_cov_4.131844, whole genome shotgun sequence.
ACCESSION   DAOOCO010000240 DAOOCO010000000
VERSION     DAOOCO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47181_length_2338_cov_4.131844"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK154_10105"
     CDS             complement(<1..313)
                     /locus_tag="PK154_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW11450.1"
                     /translation="MPLCSSPVYPVRIPFSVPSPSGPVSRYVFSFLIPTMTSLLLIDA
                     GVYGSEQRIFGEIRDLGRKPEEIRLLILTHSHPDHIGGAMAIQEATGCSIAAHPAEQQ
                     WI"
     gene            complement(501..779)
                     /locus_tag="PK154_10110"
     CDS             complement(501..779)
                     /locus_tag="PK154_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11451.1"
                     /translation="MGIFRFENKYAAPSRDQRERYMRSEAEEHHFGPEGEITLILYSN
                     AAYLKDDTDGIRILYTGSHDKIRAVEDAAAMVEYHRTRSESRDSFHHG"
     gene            921..1382
                     /locus_tag="PK154_10115"
     CDS             921..1382
                     /locus_tag="PK154_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11452.1"
                     /translation="MKCIIAILAVFCIALLMVAGCTTPMEPHTTPDTTAPTVTPTVPP
                     DLMPEPTDIVPPYQQVSIQISKNTVATDPWISVVFAGGGGQSYVVSMQATVIRSDGGI
                     ETEIAQSPGIGTTLMFAGTTRDDRAIVNVTYTDGMTYTVKDELVPFQNINP"
     gene            complement(1402..>2338)
                     /locus_tag="PK154_10120"
     CDS             complement(1402..>2338)
                     /locus_tag="PK154_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW11453.1"
                     /translation="FKDIERIASKYGTVYALYSESDTIEVGDEERRATIYGLDQEVIP
                     EIMGLASGAYPKSTSSVIIGPSLAGRLDLTLGSKIDIGDPDENATITVRVVGIIEERG
                     MAMDLNTDSAIIGSEKLFTGIYGGEGEYRQVNIILDDIDDAGIVSTEIEEQLNKKEEQ
                     VSVQDSSRMLENITETLGTMTTFVMAIAGISLLVAAVSIFNVMMMSVTERIREIGILR
                     SIGTQKGEVRKMFIYESAILGGLGAVIGAVASLTIGWVVVLAMVGSTDYFFAPASLMY
                     VPFGMAIGFITCVLSGVYPAWSASNLDPIEALRAE"
BASE COUNT          549 a          647 c          569 g          573 t
ORIGIN      
        1 caatccattg ttgttcagcc ggatgggctg caatgctaca tccggtggcc tcctggattg
       61 ccattgctcc cccgatatga tcaggatggg aatgcgtcag gattagtaat cttatctctt
      121 ccggtttcct gccgagatcc cggatctcac caaatattct ctgctccgac ccgtacaccc
      181 cggcatcaat gagcaggagg gatgtcattg tcgggatcag aaacgagaaa acgtaacgcg
      241 aaacggggcc tgatggagaa gggacgctga acggtatcct gaccggatag acgggggaag
      301 aacagagggg catggcgatt tgtcctcctt tgttcttatt tacatgctct ccttaaatag
      361 aacattcctt gtttattgat gagaatcatg ataaaaagcc tctatgggat aatttgacag
      421 aggaagctgc gatccctcat gcaattcttc gatccattcg gcagacaaca ggagatgatt
      481 agaaaattac tctcacgaca ttacccgtgg tgaaacgaat ctctggactc tgacctggtc
      541 ctgtggtatt ccaccatcgc ggccgcatcc tcgactgccc tgattttgtc atggcttccg
      601 gtgtacagga tcctgatgcc atcagtatcg tctttgagat atgcggcatt gctgtagagt
      661 atgagcgtga tttccccctc aggcccgaaa tgatgctctt cggcctcact tctcatgtac
      721 cgctcccgtt gatctcgtga aggtgcagca tacttgttct caaaccgaaa tattcccatc
      781 aaaacctcat ggattggaat acctgatagt ctcgcggagt ctggtgtgaa agtggaatca
      841 tttccattcc caccaaagaa acgttgaata gaacaaagcg gttcagaaaa aatttaacgg
      901 atgttcgcta tgaatagtgc atgaaatgta tcatcgccat ccttgcggta ttctgcattg
      961 cacttctcat ggtggccggc tgcacgaccc caatggaacc tcataccact ccagacacga
     1021 cagctccgac agtcactcct acggtgccgc ctgatctcat gccggaaccg accgatatcg
     1081 tcccccctta ccagcaggta tcaatccaga tctccaagaa caccgttgcc actgatccct
     1141 ggatctccgt ggtctttgcc ggtggtggcg gccagtccta tgtggtcagc atgcaggcaa
     1201 cagtcatacg atctgatgga ggcatcgaga cagagattgc ccagtcacct ggaatcggga
     1261 ctacactgat gtttgccggg accacgagag acgacagggc tatcgtgaat gttacttaca
     1321 ccgatgggat gacgtacacg gtgaaagatg aactcgttcc gttccagaat atcaacccct
     1381 gatctttttc tccattctga atcactccgc acggagcgct tctatcgggt cgagattcga
     1441 cgcactccat gccggatata ctccagagag gacgcacgtg atgaatccga tggccattcc
     1501 aaacgggacg tacatgaggc ttgccggggc aaagaaatag tcagtgctgc cgaccattgc
     1561 aaggaccacg acccatccga tagtgagact tgccacagcc ccgatcactg ccccaagacc
     1621 cccgagaatg gcagattcgt agatgaacat cttacgcacc tcgcccttct gggtcccgat
     1681 gctccgaagg atacctatct ccctgatccg ctcggtgacc gacatcatca tcacgttgaa
     1741 gatggagacc gctgcgacga gcagggaaat cccggcaata gccataacaa aggtggtcat
     1801 tgttccaagg gtctcggtaa tattctcgag catgcggctg ctgtcctgga ccgagacctg
     1861 ctcctctttc ttgttcagct gctcctcaat ctcggtgctg acgatcccgg catcatcgat
     1921 atcatcgagg atgatattga cctggcggta ctccccttct ccaccataga ttccggtaaa
     1981 cagcttctcg ctcccgatga tggccgaatc ggtgttcagg tccatggcca tccccctttc
     2041 ctcgatgatt ccgaccactc gtactgtgat ggtggcattt tcgtcaggat cgccgatatc
     2101 gatcttgctt ccgagtgtca ggtccaggcg tccggcaagg gaggggccga taatgacgga
     2161 gctggtgctc ttggggtatg ctcccgaggc aagacccatt atctccggga tgacctcctg
     2221 gtcaagacca tagatagttg ccctccgctc ctcatctcct acttcgatgg tatcggattc
     2281 ggaatagagg gcatataccg tcccatattt cgaggctatc ctctcaatat ccttgaac
//
LOCUS       DAOOCO010000241         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_48887_length_2287_cov_3.931900, whole genome shotgun sequence.
ACCESSION   DAOOCO010000241 DAOOCO010000000
VERSION     DAOOCO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48887_length_2287_cov_3.931900"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK154_10125"
     CDS             <1..671
                     /locus_tag="PK154_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW11454.1"
                     /translation="ESADPGEWERTYQTFRQNEGLELPESKHSTPSRQFLISDLHLGH
                     GNIIHYCRRPFFSPDEMDDVLIQNWNNRVGIADEVFYLGDLCHGRDALPAAAYLARLN
                     GTIHFIAGNHDEVIPGSVTSMMIHSGATQFCLIHDPAQAPLNFPGWVIHGHHHNNDLE
                     TYPFINARTRTVNISAELLDFIPISIDELCTILKQIKPGEKVGTLKEARDRIYQGQLP
                     DDSL"
     gene            complement(891..1283)
                     /locus_tag="PK154_10130"
     CDS             complement(891..1283)
                     /locus_tag="PK154_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW11455.1"
                     /translation="MKLVVTFSRKAGKKPIIAQVVRDTGVLINVERALIDSSEGEALI
                     DIPDESCALVKERLADLGAAVRVLEDIISLDESRCVDCGACISICPQEVFSFDDDWRL
                     RMDEKKCVLCGRCIEICPHRALSHKGEG"
     gene            complement(1280..>2287)
                     /locus_tag="PK154_10135"
     CDS             complement(1280..>2287)
                     /locus_tag="PK154_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPW11456.1"
                     /translation="TDRSLRTYMGLLLPNCGNITYSGSGLLNPLANDPHFRMIGGGVP
                     AFLGGAPGMVVGEGTQHSPAGGFGTLMVTADMKQMSNDYIRAATMAGYGVTLYMGLGI
                     PIPVLDIEAVRATAIRDEDIIVDVIDYGIPTRSRPSLRKVSYAELKSGVVDLNGEEVR
                     TSSLSSFRKAREIAGVVKSWVEQGILTPVQPARPIDPLKSAMPLRETTRGPRVLDIMD
                     RKVIAIREDEEIRAAAQKLLKGETNHLPVINTAGKLVGIVTTYDISKAVVSPGKASLV
                     RDIMKKKVITTTPEEAVDVAVRKLEQYNISALPVVDGEKRVIGMLTAMNLGKLFGRWL
                     K"
BASE COUNT          496 a          704 c          593 g          494 t
ORIGIN      
        1 gagaatcggc agacccgggc gaatgggaga ggacctatca gacgttccgg cagaacgaag
       61 ggctggagtt gcccgagagc aagcatagca ctccatcccg gcagttcctg atctccgacc
      121 tccatcttgg ccatggaaat atcatccatt actgccgccg gccattcttc tcccccgatg
      181 agatggatga tgtccttata cagaactgga acaaccgggt cggcatcgcc gatgaagtgt
      241 tttacctcgg cgacctctgc catggcaggg acgcgcttcc ggcagccgca tacctcgcac
      301 gcctcaatgg aactatccat ttcattgcgg gaaaccatga cgaagtgatt cccgggtccg
      361 tcaccagcat gatgatccac agcggggcta cgcagttctg cctgatccat gatccggcac
      421 aagcacccct gaattttccc ggatgggtaa tccatggcca tcaccacaac aacgacctgg
      481 aaacatatcc tttcatcaac gcccgcaccc ggacggtcaa catcagcgca gagctgctgg
      541 attttatccc gatcagtatc gatgaacttt gcactatcct caaacagatc aagcccggtg
      601 agaaggtagg taccctgaaa gaagcacggg atcggattta ccaagggcag ctccctgatg
      661 attcgctgtg agaataccat gtttatggaa tctcacctct gcatgttcca gatatgaggt
      721 caagataatc cttcacagta gcgattcatc gttcaaggat ccttaccatc cattcccagg
      781 caggaaccgg ggagcagctg cgggaggagg atccgtatca aggattcctg acacatcatc
      841 tccttatcct cacaggattc catgtgttga aaaaagggag tgtctgcccg tcatccctct
      901 cccttgtggg agagagccct gtgcgggcag atctcgatac accggccgca gaggacgcac
      961 ttcttctcat ccatccggag tctccagtca tcatcgaacg agaacacttc ctgcgggcat
     1021 atgctgatgc aggcgccaca atcaacacac cggctctcat caagcgagat gatgtcctcg
     1081 agcaccctga ccgctgcacc gagatcggcc aggcgctctt ttacaagtgc acaggactca
     1141 tccggaatat cgatcagcgc ctcgccctcc gatgaatcga tcagcgccct ctcgacattg
     1201 atcagcaccc ctgtgtccct gacgacttgt gcgatgatgg gtttcttccc tgctttccgt
     1261 gagaaggtca cgaccagttt catttgagcc acctcccgaa cagctttcca aggttcatcg
     1321 cggtgagcat tccgatcacc cgcttctctc catcgacaac gggaagggcg ctgatattat
     1381 actgctcgag cttccggacg gcaacatcga cggcttcttc gggcgttgtc gtgatcacct
     1441 tcttcttcat gatgtccctg acaagggacg ccttgccagg gctgaccact gccttcgaga
     1501 tatcatacgt agtgacaatc ccgaccagct tccctgcagt attgataacg gggaggtgat
     1561 tggtctcacc tttcaggagc ttctgcgcag ctgcccggat ctcctcgtcc tcccggatgg
     1621 caatgacctt ccggtccatg atatcgagta cacggggtcc ccgggtggtc tcccgaagcg
     1681 gcatagcact cttcagcgga tcgataggcc gtgcgggctg cacaggggtg aggatgccct
     1741 gctccaccca tgatttgaca acccctgcga tctcgcgggc tttccggaag cttgagagcg
     1801 acgaagtccg cacctcctca ccgttcaggt ccaccacccc gctcttcagt tcggcatagc
     1861 tgaccttgcg cagcgatggg cgggaccggg tggggattcc gtaatcgatc acgtccacga
     1921 taatgtcttc atcgcggatc gcggtcgccc gcaccgcttc gatatcgagg acgggaatcg
     1981 ggatgccgag ccccatgtac agggtcaccc cataccctgc catggtggcg gcccggatat
     2041 aatcgttgga catctgcttc atgtcggctg tcaccatcag ggtgccaaag ccccctgcag
     2101 gagaatgctg ggtaccttcc ccgaccacca tccccggggc tcccccaaga aatgccggga
     2161 cccctccgcc gatcatacgg aaatggggat cgtttgccag gggattcagc agaccagatc
     2221 cggaatacgt tatgttcccg cagttcggca ggagcagtcc catgtaggtc cggaggcttc
     2281 tgtcagt
//
LOCUS       DAOOCO010000242         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_50159_length_2248_cov_2.639763, whole genome shotgun sequence.
ACCESSION   DAOOCO010000242 DAOOCO010000000
VERSION     DAOOCO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50159_length_2248_cov_2.639763"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..707
                     /locus_tag="PK154_10140"
     CDS             210..707
                     /locus_tag="PK154_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW11457.1"
                     /translation="MKIGILGDTHDNIPKLKRAVTRLNREAGQIFLHTGDFISPFVIP
                     VLSTLDGEVIGVYGNNDGDHDFLQERCRETRNVRIEGTFFEAQYGGARIALLHGHEKG
                     LLADLIESELFDVVVHGHSHRAFIMRRGETLVINPGEVCGYLTGSPTCAVYDTERGEG
                     RILLL"
     gene            760..>2248
                     /locus_tag="PK154_10145"
     CDS             760..>2248
                     /locus_tag="PK154_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW11458.1"
                     /translation="MHKGIGILGGILVFAAIALIPLDPVTFPFPAQLAAAVTGLMVVW
                     WITEAIPIYATALVPLVLFPVLGILSPKEAAVSYADQTVFLFMGGFLIAAAMMRWNLH
                     KRIALNVITLAGSGTKKLVLGFMVATAFISMWISNTATAMMMIPIALALVSTLMPGAM
                     TSTRDEQSGERQFATCLLLGVGYAATIGGMATLIGTPPNGILVAQLSSIFPEAPEIDF
                     FSWMVFAVPFMVLFLLIAWFWLTSVTHRHMPGMITGSRDVIVHELKELGPLKRGEQWT
                     LLIFISVAIAWVMRTPKEIGSLVIPGITTYIPGVTDATIAICGALLLFMLPVDGEKGI
                     FTLDWESAKGIPWGVLILFGGGICLSEGIIASGLAEVIANSFTLLAVLPLIVIILLIS
                     LFITFLNEVVSNTAMASIMVPLMAITSISMGINPMMLMVPAALASSLGFMLPVGTPPN
                     AITYGTGYISTREMARAGFALNIIGCILITLFMTFIIPSALGMSTE"
BASE COUNT          466 a          624 c          625 g          533 t
ORIGIN      
        1 cgatacctcc tttccaccga tcctcatctg catgcagaga gagtgtgcga gaatgccatt
       61 taaccgtacc gtgagggaaa ccgcaccttg agggaaaccg taccttgagg aaaaccatac
      121 cgtgaaaaca ggagtgagaa cagggggcag gtgctccgta actattttac gtgccgggca
      181 tccacacaag agctgaggtc gacatctcgt tgaagatagg tattcttggc gatacccacg
      241 acaatatccc gaagctgaaa agagcggtga cccgcctgaa tcgtgaagca gggcagatct
      301 ttctccatac cggtgatttt atctcgccgt ttgtcatccc ggtgctctct acccttgacg
      361 gtgaggtgat tggggtctat gggaacaatg acggcgacca cgatttcctt caggagcgat
      421 gcagagagac caggaatgtc cgcattgagg gcacattttt cgaggcccaa tacgggggag
      481 ccaggatagc gctccttcat ggccatgaaa aagggctcct ggccgacctg atcgagagcg
      541 aactttttga cgtcgtggtg cacgggcact cgcaccgggc gtttatcatg agacgtggag
      601 agacccttgt gatcaatcct ggtgaagtct gcggctacct gaccggaagc ccgacctgtg
      661 ctgtgtatga cacggagaga ggtgaaggca gaatcctcct gctctaaagg aagatgaata
      721 gtacaggttc atagttgatt aagtcatacc tatcccagca tgcacaaagg gatcgggatc
      781 ctcgggggaa tccttgtctt tgcggcaatt gccctcattc cgctggatcc tgtgacgttc
      841 ccctttccgg cacagcttgc agcggccgtg accggcctga tggtggtctg gtggatcact
      901 gaggcgattc cgatctatgc cacggcactt gtgccgctcg tcctgtttcc tgtcctcggc
      961 atcctctccc cgaaagaagc ggcggtctcg tacgccgatc agacggtttt tctgttcatg
     1021 ggcggctttt tgatcgctgc tgccatgatg cggtggaatc ttcacaaacg gatagcgctc
     1081 aatgtgatca cccttgccgg ttcagggaca aagaagctcg tgctgggctt catggtggct
     1141 accgcgttca tctccatgtg gatctccaac actgcgacgg cgatgatgat gatcccgatc
     1201 gcgctggccc tggtctcgac cctgatgcca ggcgcaatga ccagtacacg ggacgaacag
     1261 agcggtgagc ggcagtttgc cacctgtcta ctgctcggcg tcggttatgc tgcaaccatc
     1321 ggggggatgg caacgctcat cgggactccg ccaaacggga tcctggttgc ccagctctcc
     1381 agcatattcc cggaagcacc cgaaatcgat ttcttctcct ggatggtctt tgccgtcccc
     1441 ttcatggtcc tgttcctcct catcgcatgg ttctggctca cttcagtcac tcaccggcat
     1501 atgccaggca tgataaccgg gagcagggac gtgatcgtgc acgagttgaa agaactcggc
     1561 ccgctgaagc ggggtgagca atggaccctc ctgatattca tctcggttgc catcgcctgg
     1621 gttatgagga ccccaaagga gattggctcc ctggttattc cgggcatcac cacgtatatt
     1681 cccggggtta ctgatgccac tatcgcaatc tgcggagcgt tgctcctttt catgctgccc
     1741 gtggatggag aaaaagggat ctttacgctc gactgggaat cggcaaaagg gataccctgg
     1801 ggagtcctga tcctgttcgg aggcgggatc tgtctttcgg agggaatcat tgcaagcggg
     1861 cttgccgagg tgattgccaa ttcgttcacc cttcttgcgg tgctccctct tatcgtgatc
     1921 atcctcctga tctccctctt catcaccttc ctgaacgagg tggtttcaaa cacggcgatg
     1981 gcatcgataa tggtcccgct gatggcgatc acatcgatat cgatggggat caatccgatg
     2041 atgctgatgg ttcccgctgc actcgcctcg tcactcggtt tcatgctccc ggtggggacc
     2101 ccgccaaatg cgatcacgta cggcacaggc tatatctcca cgagagagat ggccagggct
     2161 ggttttgcct tgaacatcat cggctgcatc ctgatcaccc tcttcatgac gttcatcatc
     2221 ccgtcagcac tgggaatgtc cacggaac
//
LOCUS       DAOOCO010000243         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_51445_length_2212_cov_5.079740, whole genome shotgun sequence.
ACCESSION   DAOOCO010000243 DAOOCO010000000
VERSION     DAOOCO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51445_length_2212_cov_5.079740"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK154_10150"
     CDS             complement(<1..413)
                     /locus_tag="PK154_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11459.1"
                     /translation="MKSDKKGKPVGKKGDSTKSWAKMFLVLGCILFVGVMIITSLGTN
                     WLVTMNPAKTGDTAIIDLTIRDSQGRPVLTTNARIFNTSYEKGEVVWYTNPLTMAVNS
                     TSTEMISPLPVYRYDYGEASFALFGDELSQISSAL"
     gene            546..1013
                     /locus_tag="PK154_10155"
     CDS             546..1013
                     /locus_tag="PK154_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPW11460.1"
                     /translation="MYRRRYPFGWTWRDLDEMMTEMENRFWGPSGRLLPPGGIADRMM
                     PALRGEFRVDVRDHDDEVIVVADLPGVEKADLSVSLVNPATLEIRSERKAETEEKEEG
                     YFMKERMSGAMSRIVALPHDVTDADASATFKNGVLEIRLKKSTTERGSPIKVE"
     gene            1076..1861
                     /locus_tag="PK154_10160"
     CDS             1076..1861
                     /locus_tag="PK154_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW11461.1"
                     /translation="MDKKKVRDYMTYDVITIDVHATTREVMEKIQKTGHDGFPVINDS
                     EVVGYVSARDLLFVPPATPVEKVMSQHLIVADPDMSVNDAARVIFRSGIQKLPVVDQE
                     NKLLGIISNTDVIRSQIEHVSPEKVFNFITTLNKLHGINPELKREVVPIDQLIPTQSK
                     IYEDELEGRMYEIRKGLAEPLIVIRRPGRLILVDGHHRAIAAKKLGIKTLDAYVIEVN
                     ENIELGMERTAKAMNLRNLDDIQVLDYARHPLVAITHRLVRRA"
BASE COUNT          573 a          553 c          576 g          510 t
ORIGIN      
        1 tcgagagctg aggatatctg gcttaattca tcgccaaaga gagcgaagct cgcctcccca
       61 taatcatagc gatagaccgg gaggggggag atcatctcag tggaggtgga attgaccgcc
      121 atcgtgagag gattggtata ccagaccacc tctcctttct catagctggt attgaaaatc
      181 cgcgcattgg tggtgagcac tggccgtccc tgcgagtcgc ggatcgtcag atcgatgatc
      241 gcggtatcac cagtttttgc aggattcatg gtaacgagcc agttcgtccc gagagaggtg
      301 atgatcataa caccgacgaa taaaatacag cccaatacca ggaacatctt tgcccatgat
      361 ttggtactgt cgcccttttt gccaaccggt tttccttttt tatccgattt cattcatttt
      421 taatggaatt ctttgggaga taaacattcc attcggttca gggaaatgca caaatattaa
      481 tatactataa acattaactt aagggtagtc cacctgtgtc gggtgggtga ggaggaatgc
      541 caagaatgta tcgcaggaga tatccattcg gatggacctg gagagacctt gatgagatga
      601 tgactgagat ggagaaccgg ttctggggcc cttcaggccg gctccttcct cccgggggga
      661 tcgctgaccg catgatgccc gcactccggg gcgagttccg ggtcgatgtc cgcgaccatg
      721 atgacgaggt catcgtggta gccgacctcc ccggggtaga aaaggccgat ctctcagtct
      781 cgctcgtcaa tccagcaaca cttgagatcc ggtcagagcg gaaggccgag acggaggaga
      841 aagaggaagg atactttatg aaggagagga tgagcggggc tatgagccgt atcgtcgccc
      901 tcccgcatga tgtcaccgat gccgatgcat cggccacgtt caagaacggt gttctcgaga
      961 tcaggctgaa gaagagcacg acagaacgcg gttctcccat aaaagttgag taatcccacc
     1021 tgcatctccc ttttttccga ttatttgaag tgatctgaac cggaacctac atgcaatgga
     1081 caagaaaaag gtcagggact acatgaccta cgacgtgatc accattgatg tgcacgctac
     1141 cacgcgcgag gtcatggaga agatccagaa gacagggcat gatgggtttc ctgtcatcaa
     1201 cgacagcgag gtggtgggat acgtctctgc ccgggatttg ctcttcgtcc cgcctgccac
     1261 accggttgag aaggtgatgt cccaacacct gatcgttgcc gatcctgaca tgagcgtgaa
     1321 cgatgcagcc cgggtcatct tccggtcagg gatccagaaa ctccccgtgg tcgaccagga
     1381 gaacaaactg cttggcatca tctcgaacac cgatgtgatc aggtcccaga tcgagcatgt
     1441 atcaccggag aaagtcttca atttcatcac caccctgaac aagctgcacg ggatcaatcc
     1501 tgaactgaaa cgggaggtgg tcccaatcga ccagctgatc ccaacccagt caaaaatata
     1561 cgaggatgaa ctggaaggga ggatgtacga aatcaggaag gggctggcag aaccgctgat
     1621 cgtgatccga cgtccgggca ggctgatcct cgttgacggt catcaccggg ccattgccgc
     1681 aaagaaactc gggataaaaa ccctggatgc ttatgtgatc gaagtgaacg agaacatcga
     1741 gcttggcatg gaacggaccg caaaagcgat gaacctgaga aatcttgatg atatccaggt
     1801 ccttgactat gcccgccatc cccttgtcgc catcacccac cggctcgtcc gacgggcctg
     1861 atgcgaaaag atctgggcag gagtaagggt aacccgatgt ttcggggcct cttgtgaatt
     1921 ataccctcaa tttttatctg accttgattt ttatttgaaa atacatcccg cagtttcatc
     1981 ttgtacgcct gtgccccaca agggaatcgt gtcatcaatt tggggattgt ttttattttc
     2041 accaccaaca tttctcataa atcacatcgg gaaacaaaca gcagccatag ctctcgcatc
     2101 tattcagaaa gtgatgacca ttctccatgg accattctcc atgaaatatg aaaaaaatgg
     2161 ctctgttgac atcctagtca actgacagta ttgcctggtt tttcaggaag cg
//
LOCUS       DAOOCO010000244         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_51494_length_2211_cov_4.320501, whole genome shotgun sequence.
ACCESSION   DAOOCO010000244 DAOOCO010000000
VERSION     DAOOCO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51494_length_2211_cov_4.320501"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..357
                     /locus_tag="PK154_10165"
     CDS             124..357
                     /locus_tag="PK154_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11462.1"
                     /translation="MIKLAEKAKAGEKVGPGKYVCIDCGREMTLDKSEQDLRKCPQCA
                     CEEYQCFPMHHIRPDIKTPEDAMNPPKRGKSNQ"
     gene            518..646
                     /locus_tag="PK154_10170"
     CDS             518..646
                     /locus_tag="PK154_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HPW11463.1"
                     /translation="MVNVSKEGQVFECEICGNVVVVKEVGGGELVCCGQPMNLIQG"
     gene            652..996
                     /locus_tag="PK154_10175"
     CDS             652..996
                     /locus_tag="PK154_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPW11464.1"
                     /translation="MAEDIKDLEKMIGKVPKFFKELTVNDPKMFEMVMKLEGHIWDDG
                     ALSRKSKKLIAIAIAASLRDQHAVHAQLIGASKLGISKEEVEEALRVAFLLAGMPAYV
                     YGKTQLDEVMKE"
     gene            1003..1644
                     /locus_tag="PK154_10180"
     CDS             1003..1644
                     /locus_tag="PK154_10180"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW11465.1"
                     /translation="MCGESNAMPILGEPAPDFEALTTNGMVKLSDFKGKWLVLFSHPA
                     DFTPVCTTEFIAFSAIADELKSLNVQLMGLSIDSVHSHLAWIRNVKEKMGVDIPFPII
                     ADLDMNVAKKYGMIHPGQSSTAAIRAVFFIDDKGILRAMIYYPLTAGRYMPEIIRLVK
                     SLQATDKYGIATPANWQPGDKVVVPAPKNKAEMDKRLSEGYECKDWYLCFKKL"
     gene            1766..>2211
                     /locus_tag="PK154_10185"
     CDS             1766..>2211
                     /locus_tag="PK154_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPW11466.1"
                     /translation="MRIKVLAFAGSPRRHGNSEALLDWVLDAMQKEEDADIEKVILVD
                     ADVNPCRGCNACETLNRCVQRDGMDTLHDKIIDADLIILSAPIYCMGICSQAKALIDR
                     FQVFRSRKYVLKLPIVPPERKGKRIGIFLSSAGQNWDHVFNGALPTV"
BASE COUNT          583 a          544 c          583 g          501 t
ORIGIN      
        1 atagctttgg aaccccccgc tctgaacatt tgtcgtttat tcctgcccga ttttaacatt
       61 ttgcaccgtt agaatggaac gataaaaagt cataaataag tcccccatct atatatccgt
      121 aaggtgatta aactggcaga gaaagcaaaa gccggagaga aagtcggtcc tggtaagtac
      181 gtctgcatcg attgcggtcg ggagatgact ctcgataagt ccgaacagga tctcaggaag
      241 tgtccgcagt gtgcctgtga ggagtaccag tgcttcccga tgcaccacat ccgcccggat
      301 attaagaccc ccgaagatgc gatgaacccc ccgaagcggg gcaagagcaa ccagtaacgt
      361 attgttttca acacggttgt gggaggacag ggcagatcaa agatctaccc tgaacagaga
      421 aacccataac tttttcttat caatacagga tacagggaac tgccctgacc ggtacattca
      481 tgagctgtac ggacaagtca gaaagaggtg ttccagaatg gtaaatgtct ccaaggaagg
      541 acaggttttc gagtgtgaaa tctgtggaaa tgttgttgtg gtcaaggaag tcggcggtgg
      601 cgaactggtc tgctgcggcc agccgatgaa tctcatacag gggtgatccc catggcagag
      661 gatatcaagg atctggagaa gatgatcggc aaggttccga aattcttcaa ggaactgacg
      721 gtgaacgacc cgaagatgtt tgagatggtg atgaagcttg agggacatat atgggatgac
      781 ggagccctct ccaggaagag caagaaactg attgccatcg ccatcgctgc atcgctccgg
      841 gatcaacatg cggtccatgc ccagctgatc ggtgcttcca agctcgggat ctctaaagaa
      901 gaggtcgagg aggcactcag ggtggcattt ctcctggcag gaatgcctgc gtatgtctac
      961 gggaagacac agctcgatga agtcatgaag gagtgagaaa cgatgtgtgg tgaatccaac
     1021 gcaatgccaa ttcttggtga gccagccccc gactttgagg cactaaccac aaatggcatg
     1081 gtgaagctct ctgatttcaa ggggaagtgg ctggtgctgt tctctcatcc cgcggacttt
     1141 acccccgtat gcacaacaga gttcatagca ttttcagcga ttgcggatga gttgaaatcg
     1201 cttaatgtgc aattgatggg gctctctatt gactctgtgc attcacatct tgcctggata
     1261 aggaacgtga aagagaagat gggagtcgat attccgttcc ccatcatcgc cgaccttgac
     1321 atgaatgttg ccaaaaagta tgggatgatc cacccgggcc agagcagcac ggctgcaatc
     1381 agggcagtat ttttcatcga tgacaaaggt atcctgcggg ccatgatcta ctatccgctc
     1441 acggcagggc ggtacatgcc ggagataatt cgcctcgtaa aatccctgca agccaccgac
     1501 aaatacggca tagccacccc tgcaaactgg cagcctggtg acaaggtcgt tgtccctgcg
     1561 ccaaagaaca aggccgagat ggacaaacgg ctttcggaag ggtacgagtg caaagattgg
     1621 tacctctgct tcaagaaact gtaactgcaa tttctctttt ttagggtagt cttccaaccc
     1681 acaagaccgc aattccccca acgttttaaa agatcctcat gcttcatcca accatttaag
     1741 tcggacgcct ccccacttga gacgtatgcg gatcaaagtc ctggccttcg caggaagtcc
     1801 ccggcggcat ggcaactcag aggcactcct tgattgggtc cttgatgcaa tgcagaagga
     1861 agaagatgct gacattgaga aggtgatcct ggtcgatgcc gatgtcaatc cctgcagggg
     1921 ttgtaatgcc tgcgagaccc tgaaccggtg tgtccagcgg gacggcatgg ataccctcca
     1981 tgacaagatt atcgatgcag atctgatcat tctctcagca ccaatctact gcatgggcat
     2041 ctgttcccag gcaaaagcct tgatcgatcg cttccaggtc ttccgatcac gaaaatatgt
     2101 cctgaaatta ccgatcgtcc ctcccgaacg gaaagggaag cggatcggga tattcctctc
     2161 ctctgccggg cagaactggg accatgtatt caatggggca cttcctaccg t
//
LOCUS       DAOOCO010000245         2173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_52927_length_2173_cov_4.288008, whole genome shotgun sequence.
ACCESSION   DAOOCO010000245 DAOOCO010000000
VERSION     DAOOCO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52927_length_2173_cov_4.288008"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /gene="rpl12p"
                     /locus_tag="PK154_10190"
     CDS             <1..266
                     /gene="rpl12p"
                     /locus_tag="PK154_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="similar to eukaryotic L12; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HPW11467.1"
                     /translation="ESVKAVLTAAGIAVDDARIKALVAALDGVNIEEAISKAAVAPVA
                     VAVAPSAAAAAPAAAAQAAAAPAPEDKKEEEESGMAGLGALFG"
     gene            complement(702..1250)
                     /locus_tag="PK154_10195"
     CDS             complement(702..1250)
                     /locus_tag="PK154_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HPW11468.1"
                     /translation="MDLLIFWDSGAPAGLELPVMRMVSGVLEIPTRVCANPLILNGYV
                     GSRHQTDASVLLDTIDMFKQRQGIRNPILVVTGNDIFFTGTTSLFGLARPSAGAAVVS
                     TARLTNEFYGRDGDEDDLIDRTVKESAHEIGHLFGLDHCMTPECIMHNPLTLDDLERK
                     KRWFCPECKGLLETANLMHSLS"
     gene            1267..1689
                     /locus_tag="PK154_10200"
     CDS             1267..1689
                     /locus_tag="PK154_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HPW11469.1"
                     /translation="MGTDKHIERCIGVYIVSCYHSAAEVGVGSNFTAAETLSKSGVHV
                     FCTDIVPPRQSSQVPFFIDDIFSPDIRLYEGIDLIYSIRPGEEMVPPLIRLAEGIDVD
                     LLIYHLGFEGYGRGGEIIECGVPLHRYHTSQNPSKRVF"
     gene            complement(1662..>2173)
                     /locus_tag="PK154_10205"
     CDS             complement(1662..>2173)
                     /locus_tag="PK154_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11470.1"
                     /translation="KIGSYRKQNMVRTAMLAKIGKSLHLSQYMLRDSYLTPFSLLADR
                     DPLEFARAFSLDADELNLLIHDKPRSQKVVKNLIDEERVKERERVKAEKLKGKKESEP
                     SAVGGRSAGPLPIPPNEPAPGQDTIGLPKSPLPGSESTSEIRKDGKNASGDEKPEEKR
                     TQKTLFDGF"
BASE COUNT          493 a          556 c          557 g          567 t
ORIGIN      
        1 aagagagtgt aaaagcggtc cttaccgctg ccggtatcgc tgttgacgac gcccgaatca
       61 aggccctggt agcagccctc gatggagtga atatcgaaga ggctatcagc aaggctgccg
      121 ttgccccggt tgctgttgca gtggctccgt ctgctgctgc agcggctccg gctgcagccg
      181 cacaggctgc cgcagccccg gctccggaag acaagaagga agaagaagag agcggcatgg
      241 cagggctcgg tgccctgttc ggctgaaaca ccttttttac aataaaaatg cctcaggcat
      301 tttcacacgt tatgctcgta tgtacgtcgt tcattgattg ttcgaatgac aatcacttcg
      361 tgattttcat aaattatgcc ggctcccgaa atcggggctc ataggcggtt ccaatgaaaa
      421 tgcctccggc attatcatct cttgcgtatc tccctcaagg gactcctcta actctccaaa
      481 tccctgtcaa tccttcagat accctttaat gctacctgat cctgtttttt caatatcctc
      541 catttccgtg agaagggatc cctgacgtag cctttcagtg caatgaagag gtagtgcctg
      601 atgttccgta cgtaatctca atcgctcctg gtatctggtg ctcacattgc cagttggttc
      661 cagggaagca aaaggagtag gggaggaagg agaggaagga ttcaggagag ggaatgcatc
      721 aggttggcag tttcgaggag acccttacat tccgggcaaa accagcgttt tttacgctcg
      781 agatcgtcaa gggtcagggg attgtgcatg atacattccg gcgtcatgca atgatccagg
      841 ccgaagaggt gccctatctc atgggcactc tcctttactg tccggtcaat aaggtcatcc
      901 tcgtcaccgt ctctcccata gaactcattg gtaagccgtg ctgtggacac gactgcagcc
      961 cctgcagaag gacgggccag gccgaacaat gaggtggttc cggtgaaaaa tatgtcgttc
     1021 ccggtgacta ccaggatagg gttcctaata ccctggcgtt gcttgaacat atcgatcgta
     1081 tccaggagga cactggcatc tgtctgatga cgggatccca catacccgtt caggatgagt
     1141 ggatttgcac ataccctcgt gggaatttca agcacccctg ataccatacg catgaccggg
     1201 agctcgaggc ctgcaggtgc tccggaatcc caaaaaataa ggagatccat caccaatata
     1261 ctctgaatgg gcactgataa acatattgaa cgctgtatcg gggtctatat tgtgtcctgt
     1321 taccattcag cagctgaggt gggggtagga tccaatttta cagccgctga gacactcagt
     1381 aaatcaggtg ttcacgtatt ctgcaccgac attgtgcctc cccgacagtc ttcccaggtc
     1441 ccgttcttta ttgatgacat cttctccccg gatatccgtc tgtatgaagg tattgatctg
     1501 atatattcga tccgtcccgg agaagagatg gtaccacccc tgatccggct tgcagaagga
     1561 atcgatgtcg atctcctcat ctatcatctt ggatttgaag gatatggccg gggcggtgaa
     1621 ataatcgaat gcggagttcc ccttcaccgg tatcatacaa gtcagaaccc gtcaaagaga
     1681 gttttttgag tacgtttttc ctccggtttc tcatcgccgg atgcattttt cccgtctttt
     1741 cgtatttcac tggtggattc actgccggga agaggagatt tcggcagccc aattgtatcc
     1801 tgtccgggag cgggctcgtt gggggggatc ggcagcggcc ctgcacttct tccccctaca
     1861 gctgaaggtt cagactcttt ttttcccttc aatttttcgg cttttacgcg ttctctctcc
     1921 tttacccgct cctcatctat cagatttttt accactttct gtgatctggg cttatcatgg
     1981 atcaggaggt ttaactcatc ggcatcaaga gagaacgccc gggcgaattc cagggggtcg
     2041 cgatcggcca ggagggaaaa cggtgtcagg taggaatcgc gaagcatata ttgggagaga
     2101 tgaagagatt tgccaatctt tgcaagcatc gcggtacgaa ccatgttctg cttccggtaa
     2161 ctgcctatct ttc
//
LOCUS       DAOOCO010000246         2161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_53388_length_2161_cov_4.316239, whole genome shotgun sequence.
ACCESSION   DAOOCO010000246 DAOOCO010000000
VERSION     DAOOCO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53388_length_2161_cov_4.316239"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="purD"
                     /locus_tag="PK154_10210"
     CDS             <1..1022
                     /gene="purD"
                     /locus_tag="PK154_10210"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW11471.1"
                     /translation="CIGPTLKAAMLETDKAFCRAMMHRHGIAGCPHYRTFYHADDAIR
                     FIRNYDGDLAIKPIGLTGGKGVRIMGEHLDREGAVAYVRSLEGRVVLEERLIGEEFTL
                     QAFVDGKHIVPMPLVQDHKRAFEGDLGPNTGGMGAYSMPDHSLPFVTDSVRTQAFGIM
                     KEVVKVMGESGTPYRGVLYGQFMNTARGPMVIEFNARFGDPEAMNVLSLLESDLSSII
                     TRMISGSLSEKDVKFARKATVCKYIVPEGYPDAPRTGEPVVLGDNTPDTLLYYANVEE
                     REGTLYTQSSRTLAFVGIGDTLDAAEHSAESAARSVKGRVRYRRDIGTAALLQKRISH
                     MKELR"
     gene            1019..1939
                     /gene="argF"
                     /locus_tag="PK154_10215"
     CDS             1019..1939
                     /gene="argF"
                     /locus_tag="PK154_10215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPW11472.1"
                     /translation="MKKDLLSILDLSRDELREILDDADQLKILRKEGRPHEFLYGKAL
                     ALVFEKASTRTRISFEVGMAELGGHALFLNPQDLQIGRGEEIRDTARVMSRYVSGMMI
                     RAYKHSTIEEFARNSSVPVINGLSDIEHPCQILADIMTIREHFRSIEGLKVTWVGDGN
                     NVCNSLVLASARAGFEITVASPEKYRISEAVVKRAVAEGGNITLLKDPSEAVKDAHVV
                     VTDTWISMGEESERDARIRAFKGFTVDTGLMKKADPDAIFLHCLPAHRGREVTEEVIE
                     GPQSVVFDEAENRLHAQKAVLVKMIGREIG"
BASE COUNT          494 a          575 c          652 g          440 t
ORIGIN      
        1 cctgcatcgg gcctaccctg aaagcagcca tgctcgagac ggacaaggca ttctgccggg
       61 cgatgatgca ccggcatggc atcgcgggct gcccgcacta ccgcaccttc taccatgccg
      121 atgacgcgat caggtttatc agaaattacg acggggatct tgcgatcaag ccgatcggtc
      181 tcaccggggg caaaggagtc aggatcatgg gggagcacct ggacagggag ggagcagtcg
      241 cttatgtgcg gtcgctcgag ggcagggttg tgctcgagga acgcctcatc ggggaggagt
      301 tcaccctcca ggcctttgtg gacgggaagc atatcgtgcc catgcctctt gtccaagacc
      361 acaagcgtgc ctttgagggg gatctcggcc ccaataccgg tggaatggga gcctactcta
      421 tgccagacca ctccctcccg tttgtcacag attcggttcg cacccaggca ttcgggatca
      481 tgaaggaggt ggtgaaggtc atgggtgaat ccggaacccc ctaccggggg gtcctctatg
      541 gccagttcat gaacaccgcc cggggaccga tggtgatcga gttcaatgca cggttcggag
      601 atcctgaagc gatgaatgtg ctgtccctgc tcgaatctga tctctcgagt atcataacca
      661 ggatgatatc aggatcgctg tcagaaaaag atgtgaagtt tgcacgcaaa gcaacagtgt
      721 gcaaatatat cgttccggaa ggctacccgg atgctccacg gacaggagag cctgtggtgc
      781 tcggggacaa cactcccgat actctgctct actatgccaa tgtagaggag agagagggta
      841 ccttgtatac ccagagttcc cgaaccctcg cctttgtcgg gattggtgat acacttgatg
      901 cggcagaaca ctctgccgag agcgctgcac gatcggtgaa aggccgggtc aggtaccggc
      961 gtgatatcgg gactgcggca ctcctccaga aacggatctc ccacatgaag gagttgcgat
     1021 gaagaaagac cttctctcga tacttgatct ctcgcgggac gaactgcgtg agatcctcga
     1081 tgatgccgat cagctgaaga tcctccggaa ggaggggagg ccccatgaat ttctctacgg
     1141 gaaagccctc gccctggtat ttgagaaggc ctctacgagg acacggatct cgtttgaggt
     1201 cggcatggca gaactggggg gccatgccct cttcttaaac ccccaggacc tccagatcgg
     1261 cagaggtgag gagatcaggg ataccgcccg ggtgatgtcc cggtacgtgt ccggcatgat
     1321 gatccgggcc tacaagcact cgaccataga ggaatttgcc cgcaattcca gtgtgccggt
     1381 catcaacggg ctctcggaca tcgagcaccc ctgccagatc cttgccgata ttatgacgat
     1441 aagggagcat ttcagatcca ttgaaggcct caaggtcacc tgggtcggcg atggcaacaa
     1501 tgtctgcaac tcccttgtcc tggcctctgc ccgtgccggc ttcgagataa cggttgcatc
     1561 cccagagaag tacaggatct cggaagccgt ggtgaagcgt gccgttgcag agggtgggaa
     1621 cattaccctg ctcaaggacc catcagaggc ggtgaaggat gcccacgtgg tggtcaccga
     1681 cacctggatc tcgatgggag aggagagcga gcgggatgcc aggatccggg cattcaaagg
     1741 attcacggtg gataccgggc tgatgaagaa agcagatcct gatgcaatat tcctccactg
     1801 cctcccggcc caccggggga gggaggttac cgaggaggtg atcgagggcc cgcagagcgt
     1861 ggtctttgac gaggcagaga accggctgca tgcccagaag gcagtgctgg tgaagatgat
     1921 cgggcgggag atcggatgaa tattgccccc cctttcacta atattcctct ccttttagaa
     1981 atatatgcag aatatgtttg atcatactga actcttccag tgaagacccg agagtagttg
     2041 gcacgagaga gggatgagtc aggttgtcat agtgttgcgg gtgtggatac cacagagacg
     2101 ccgctttaca aatccctcca tccccccgtg gctctatgag aaaatgctgt caccatctga
     2161 g
//
LOCUS       DAOOCO010000247         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_53836_length_2150_cov_3.538425, whole genome shotgun sequence.
ACCESSION   DAOOCO010000247 DAOOCO010000000
VERSION     DAOOCO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53836_length_2150_cov_3.538425"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK154_10220"
     CDS             complement(<1..653)
                     /locus_tag="PK154_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPW11473.1"
                     /translation="MPGPDGLVDATPRSLGFRMPAEWERQEGVWLSWPKDQDTFPDLI
                     AVEQAYLQIIRVISRYEFVHLLVSTEEVGQYVSDIFQKERIRTEHLIMHRIEYADVWF
                     RDYGPSFITQGKDSSLAMVDWTFNAWGNKYPGLLRDDTIPSEMNKELTIPRFVPGIVL
                     EGGSIDVNGCGTVLTTEQCLLNRNRNPGLDQSRISWYLREYLGCCHVIWLKDGIVGD"
     gene            complement(658..1518)
                     /locus_tag="PK154_10225"
     CDS             complement(658..1518)
                     /locus_tag="PK154_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPW11474.1"
                     /translation="MAHGNVRIGLVQTMVSGDVEENLLRTLAFCRKAADEGARIICLQ
                     ELFRTPYFPQEEYRDATLYAEPVPGETTTRFAEFARSFKVTVIVPLFEKGEAGRYYNS
                     AVVIDEKGTIMPPYRKVHIPHDPLFYEQNYFSGGDSFRIYSCGPVKCAVLICYDQWFP
                     EAARAVTLMGADVIFYPTAIGWIKGLSDPAEGDWRVAWETVQRGHAISNGVYIAAVNR
                     WGTEGDLTFWGSSFVSDSFGNVLVRAGSNRDEVVIADISLDLNELVRSGWGFLRNRRP
                     EMYGILTRGT"
     gene            complement(1782..>2150)
                     /locus_tag="PK154_10230"
     CDS             complement(1782..>2150)
                     /locus_tag="PK154_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11475.1"
                     /translation="MTRRPKKVDSRPKKAYDQRRFRISWMVKTRRGIRTSRPFIPSMI
                     TRYREIPIRIYNRVQTGAKIQAGGLNVGFMSVEYHEPGPVVVKSDPSKPAQLQSRTDP
                     IKIPPAFVLDTLSCIGYPPM"
BASE COUNT          511 a          589 c          492 g          558 t
ORIGIN      
        1 tcatccccca caatcccgtc tttaagccag atgacatggc agcaacccag gtattccctg
       61 aggtaccacg agatcctgct ctgatcaaga ccggggttgc gattgcggtt gagaagacac
      121 tgttcggtcg taagtactgt gccgcatccg ttcacatcga ttgaaccccc ttccagaacg
      181 atacctggga caaatcgtgg aatagtgagt tccttgttca tttcagaggg gatggtgtca
      241 tccctgagca ggccaggata cttattcccc cacgcattaa aagtccagtc caccattgca
      301 agagacgaat cttttccctg tgtaataaaa ctcgggccat aatccctgaa ccagacatcc
      361 gcatactcta ttcggtgcat aatcaggtgt tcagttctta ttcgctcttt ctggaaaata
      421 tctgacacat attggccgac ttcttcagta ctgacgagca ggtggacaaa ttcatagcgg
      481 gagatcaccc tgataatctg aagatatgcc tgttcaaccg cgatgagatc agggaaggtg
      541 tcctgatcct ttggccagga gagccacacc ccctcctggc gttcccattc ggccggcatc
      601 cggaatccca gagaacgagg ggtcgcatcc accagcccat ctggtcctgg caccctctca
      661 cgtcccccgg gtaagaatcc cgtacatttc aggtctccgg ttccggagaa aaccccaccc
      721 ggatcgaacc aactcattca ggtcaagaga tatgtctgct atgaccactt cgtcacggtt
      781 actgcctgcc cggacaagga cgtttccgaa cgaatctgag acaaatgaac ttccccagaa
      841 ggtaagatct ccctcagttc cccaccggtt cacggcagcg atataaaccc cgttggagat
      901 agcatgcccc cgctggaccg tctcccaggc aacccgccag tccccttctg cagggtccga
      961 aagccccttt atccatccga ttgcggttgg gtaaaagata acatcggcac ccatgagggt
     1021 taccgcccgt gctgcttccg ggaaccactg atcatagcag atcaggactg cacactttac
     1081 tggtccgcag gagtagattc tgaacgaatc tcctcctgaa aagtaatttt gttcataaaa
     1141 gaggggatca tggggtatat gcactttccg ataaggaggc atgatcgtcc ccttctcatc
     1201 gatcaccacg gctgagttgt aataccttcc tgcttcgcct ttttcgaaaa gggggacaat
     1261 gacagtgacc ttgaaggacc tggcaaactc tgcaaatctg gtggtggtct ctcccgggac
     1321 tggttctgca tagagggtag catcgcgata ttcttcctgc gggaaatagg gtgtcctgaa
     1381 gagttcctgg agacatatga tccgggctcc ctcatcagcg gcttttcggc aaaaggcgag
     1441 ggtgcgtaac aggttctctt caacatctcc ggataccatg gtctggacaa gcccaatcct
     1501 tacgttccca tgtgccattg gtttcagcac agctgttagg cccatgatac atttaacata
     1561 tgccaaagca caggggacta ggatatcttt cctttaagga cctcttggct gcccccccat
     1621 tgttttgccg gtcacgttca taccagcatg ccattggatg aaatacgaag tcccctcctg
     1681 ggggcccttt tttgtatttc caacaggaga ggacacggat tttaatcatt cttcctcctt
     1741 gctctgaaaa aagaaaattt ttaagttact attatctggc atcacatagg aggatatcct
     1801 atgcaagaga gtgtatccaa aacaaaggca ggaggtatct taatcggatc ggtcctgctc
     1861 tgtaactgtg ctggtttgct gggatcactt ttcaccacta ccgggcccgg ctcatggtat
     1921 tcaacactca taaaaccaac attcaatccg ccagcttgga tttttgcacc tgtctggacc
     1981 ctgttatata ttcttatggg gatctccctg tatcttgtaa tcatggaagg aatgaaaggg
     2041 cgtgatgttc gtattcctct tcttgttttc accatccagc tgatcctgaa cctcctctgg
     2101 tcatatgcct tcttcggcct ggagtcaacc tttttcggtc tccttgtcat
//
LOCUS       DAOOCO010000248         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_54580_length_2131_cov_1.977360, whole genome shotgun sequence.
ACCESSION   DAOOCO010000248 DAOOCO010000000
VERSION     DAOOCO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54580_length_2131_cov_1.977360"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..605
                     /locus_tag="PK154_10235"
     CDS             147..605
                     /locus_tag="PK154_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPW11476.1"
                     /translation="MSTNRYVNILLAMLDQIRQVSLSLKSIIALSTSSIVIWLLDVFV
                     CLLVAMMFGVSIPFAVVMLAIVVGNLVKAVPLTPGGVGTYELAVAAILTAAGVEPAVA
                     TLIAVIDHLIKNLVTLGGGVIAIYLFGNWVLEAIKSAFRKELKEDDQSGV"
     gene            586..>2131
                     /locus_tag="PK154_10240"
     CDS             586..>2131
                     /locus_tag="PK154_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPW11477.1"
                     /translation="MTSPGYELQFLMVAGWLILLVLLQLAVWPALKPVLKEFAFPAAF
                     PVSLLLFALVSWYSGLLHIPVWIAFIPFACLLFWFAYKGCYRRDTFTGQGKWALVFFV
                     FFFFMLELRFINPSISFAEKFMDHAFLASIMRAPVIPPADPWFLGGSLDVYYYLGYWM
                     FGALGIISGVPSNIVFNLALPTVLANAAVGIYALAHLLSDRLHWLPLLTFLVVNPSFL
                     WQAILGKSPGAILWDSTRTIPDTINEYPAFSFVWGDVHAHVVSIFNQVLLLFLLLYVL
                     KNWKDLAPRCRLFLTGLCALSLGSMPLINTWDVLLYAPITLLCGLLIWNQNRDRGEGG
                     SAAAGREFDPVGDVFSRPWSFLLITPILSLLLYLPYYLQLNSAGIQGVGIVHTPSPVT
                     AFFLVHGLFLLIYLAYLRKDIIRMPLLLLTPVPFILAGYAAAGIAALPFAYFLLKKKK
                     NPAELLAICGLAVIILCEFFYLKDNMGEVYYRMNTVFKFYLPAWILMASSGFAMLAMM
                     LGPFVAD"
BASE COUNT          364 a          628 c          518 g          621 t
ORIGIN      
        1 ggtgtttgat atcgttaccg tagcccttct tggtgccgtc tcccttctct tcgtgttcag
       61 tgttcctgat gagtttttct ggctcatcat cctagcactc gtcctgggtg tcgggttttt
      121 tctcttcctc catcagcaac cggtttgtgt caacgaaccg ctatgtcaac atcctgctcg
      181 cgatgctcga ccagataagg caggtctcac tctcgttaaa gtccataata gccctctcca
      241 cctcctctat cgtgatctgg ctcctcgatg tattcgtgtg cctgttggtg gcgatgatgt
      301 ttggggtaag catcccgttt gcggtggtca tgcttgcgat cgtggtcgga aacctggtga
      361 aggctgtccc actcacccct ggaggggtgg gtacctatga gcttgcagtc gccgcgatac
      421 taacggcagc aggcgttgaa cccgcagtcg ccaccctcat tgctgtgatc gatcacctca
      481 tcaagaacct ggtcactctc ggcggagggg tcatcgccat ctacctattc ggtaactggg
      541 ttcttgaggc aataaaatcc gcattcagaa aggagctcaa agaggatgac cagtccgggg
      601 tatgaactcc agttcctgat ggtggcaggc tggctgatcc tccttgtcct gctccagctg
      661 gccgtatggc ccgccctcaa accggtgctg aaggagttcg cattccctgc tgcatttccc
      721 gtctccctcc tcctttttgc gcttgtatcc tggtactccg gactcctgca catccctgtt
      781 tggatcgctt tcatcccttt tgcatgcctc ctcttctggt ttgcctacaa aggatgttac
      841 cgtcgggata cttttaccgg ccagggcaag tgggcgcttg tcttttttgt cttcttcttc
      901 tttatgctcg aattacgatt catcaacccg agcatctcat ttgcggaaaa gttcatggat
      961 cacgcatttc ttgcgtcgat catgagggcc ccggtcattc cacccgccga tccctggttc
     1021 ctcggtggat cacttgatgt gtattattat cttgggtact ggatgtttgg cgcacttgga
     1081 attatatcag gcgtcccttc aaatattgtg ttcaacctgg cgctgccgac ggtcctggcc
     1141 aacgctgcgg ttgggatata tgcgctggcc cacctgctct ctgaccggct gcactggctc
     1201 cccctgctga cgttcctggt ggtcaatcca tcgtttctat ggcaggccat ccttggaaaa
     1261 tcacccggtg ctatcctttg ggacagtact cgcacgatcc ctgataccat caatgaatat
     1321 ccggctttct cgtttgtatg gggagatgtc catgcccatg tcgtctcgat cttcaaccag
     1381 gtgctcctcc tcttcctcct tttatacgtg ctgaagaact ggaaagatct ggccccacgc
     1441 tgtcgccttt tccttaccgg tctctgtgcc ctctcgcttg gatcgatgcc gcttatcaac
     1501 acatgggatg tcctcctcta tgccccaatc actctgctgt gcgggcttct gatctggaat
     1561 cagaaccggg acagggggga ggggggatcc gctgctgcgg gcagggagtt cgatccggtt
     1621 ggggacgtct tttccaggcc ctggagtttt cttctcatca ccccaatcct ttccctgctt
     1681 ctctatctcc cctactatct gcagttgaat tcggcaggga tacagggggt cggcatcgtg
     1741 cacaccccca gcccggtcac tgcgtttttt ctggtccacg gcctgttcct cctgatatac
     1801 cttgcctacc tcagaaagga tatcatccgg atgccgcttc tcctcctcac cccggtgcca
     1861 ttcatcctgg ccggctatgc tgctgcagga attgctgccc tgccgtttgc atatttcctg
     1921 cttaaaaaga aaaaaaaccc ggcagaactt cttgcaatct gtggcctggc ggttatcatc
     1981 ctctgtgagt tcttctatct gaaagacaac atgggcgagg tatactatcg gatgaacacc
     2041 gtcttcaagt tctatctccc tgcctggatc ctgatggctt cctccggttt tgcgatgctc
     2101 gccatgatgc ttggaccttt cgttgcagac c
//
LOCUS       DAOOCO010000249         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_55565_length_2107_cov_5.795322, whole genome shotgun sequence.
ACCESSION   DAOOCO010000249 DAOOCO010000000
VERSION     DAOOCO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55565_length_2107_cov_5.795322"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK154_10245"
     rRNA            complement(<1..330)
                     /locus_tag="PK154_10245"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(957..1655)
                     /locus_tag="PK154_10250"
     CDS             complement(957..1655)
                     /locus_tag="PK154_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HPW11478.1"
                     /translation="MSEEPVEILVQMAERGEIDPWNIDIVEVTDRFLSELERRKELDL
                     RISGRTLFYAAILLRMKSEYLDISGDAEDEEVPDDDEGFGIEDDFPEGAGGPIDQLER
                     EIRRRLDRKNLRKRPVTLFELILELKNAEKEQRRRQRFRSTFSMDVIDVEEVVGIAHE
                     EGYLEAATTVLCQCEETITKNGVVTLHELAALLGKRIYDVYIPLLFLMSEGKLTLWQE
                     EFFGEIMVRSCRAE"
     gene            complement(1648..>2107)
                     /locus_tag="PK154_10255"
     CDS             complement(1648..>2107)
                     /locus_tag="PK154_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPW11479.1"
                     /translation="MKYEAFMDAFKAIDANFRDIFARLTSGSGRLILENEEDPFSGGL
                     SFAVQPRDKPVHLLSALSGGEKSLTTLAFIFSIQQHIPAPFYAFDEVDMSLDGANVER
                     ISAMIRELSPTSQFITVSLRKPMIEGADRILGVTLLPDKSSFVTGVRTDV"
BASE COUNT          488 a          614 c          511 g          494 t
ORIGIN      
        1 tgcgccccgt agggcctgga ttcgtgtctc agaatccatc tccgggctct tgctcccaca
       61 acccgtaccg atcaaaggct tgttgggccg ttaccccaac aacaacctaa tcggccgcag
      121 acccatcctg tggcggcgga cctttgagct agggggcatt ccagccaccc taacctatgg
      181 ggtattatcc ccagttttcc ggggttatcc ccctccacag ggtaggttgt ccacgtgtta
      241 ctgagcagtg cgccgaggtc ttgcacctct cgactcgcat ggcttaatcg aaccccgata
      301 gcagtggcct ctggcaggat caaccagaat tcaaagataa agcacactta tactgaaaat
      361 ttcgaggaat tagcggttac caaacaaatt tccgcattgc cagtaccaac gtcagaacca
      421 aggccggcgt tgccggcgta tttggctctc catcacagag ggtataatca tacgcattat
      481 cggtatataa ccattgcgac tttgccctgc aaagcagcat ggtcatgggg attccttcag
      541 agagaatccg atcgcatcgg gggacgttca taaggccact gtgagccggc cttcatcgac
      601 cctttcagcg tatcagaacc ggagatgttc tcgcagcagg gtccggttca tcatcgtaat
      661 ttgaggaact acatgttgtt tttgatgcta tatgaacctt gttcctcatc tttcaaagga
      721 acacggttcg catcatgcaa cccggaatgc cggcaggatc gccgtaattc cagggcattg
      781 gaaccatatt aagtaaccat gactgctata aaaagacttc gccctttcca gggatctcgt
      841 gtgaacattc tggtcgaaac catactggca gggcaaaaac ccttgatcat gggcaagaga
      901 ttcgcgcaga ttttggggaa catagtgacg aaacgccatc actggaccgt tttacctcac
      961 tctgcgcggc acgaacgcac catgatctct ccgaaaaact cctcctgcca gagggtgagt
     1021 tttccttccg acatcagaaa gaggaggggt atgtagacat catagattct tttccccagc
     1081 agcgcggcca gttcatggag ggtaaccacg ccgtttttcg taatcgtctc ctcgcactgg
     1141 cacagtaccg tcgttgccgc ttccagatac ccttcctcat gcgcgatccc gaccacctct
     1201 tcaacatcga tcacatccat cgagaaggtc gaccggaacc gctgacgccg ccgctgctct
     1261 ttctctgcat tcttcagctc aaggatcagc tcaaacaggg tgaccggacg tttccggaga
     1321 tttttccgat ccagccgccg cctgatctct cgctcgagct ggtcgattgg cccccctgcc
     1381 ccttccggga aatcatcttc gataccgaat ccctcatcgt catccggcac ctcctcatct
     1441 tccgcatccc cagaaatgtc aaggtattca gatttcatac ggagcagaat ggcggcataa
     1501 aacagtgttc tgcccgatat gcggagatcg agctcctttc tccgttcaag ttctgaaaga
     1561 aaccggtctg tcacctccac gatatcgata ttccagggat cgatctctcc ccgttcggcc
     1621 atctgcacca ggatctcgac cggctcttca gacatccgtc ctgactccgg taacaaatga
     1681 actcttgtca ggaagcaggg tcaccccaag gatacggtct gccccttcga tcatcggttt
     1741 cctgagcgat accgtgataa actgcgacgt gggggagagc tcccggatca tcgcactgat
     1801 ccgctcaacg tttgctccgt caagagacat gtccacttca tcaaacgcat agaatggtgc
     1861 agggatatgc tgttgtatcg agaagatgaa ggccagggta gtgagcgact tttcgcctcc
     1921 tgaaagagct gacagcagat ggaccggttt gtcgcgcggc tgcacggcaa acgaaaggcc
     1981 cccggagaaa ggatcttctt cgttctcgag gatgaggcgt ccgctcccgc tcgtgagccg
     2041 ggcaaagatg tcgcggaagt tcgcatcgat ggccttaaag gcgtccatga acgcttcata
     2101 cttcatc
//
LOCUS       DAOOCO010000250         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_55757_length_2103_cov_2.313965, whole genome shotgun sequence.
ACCESSION   DAOOCO010000250 DAOOCO010000000
VERSION     DAOOCO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55757_length_2103_cov_2.313965"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PK154_10260"
     CDS             <1..1509
                     /locus_tag="PK154_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HPW11480.1"
                     /translation="VATGDLVIVKPGGKIPVDGEVTGGESYVDESMITGEPIPVLKQI
                     GSKVIGGTLNTNGIFTFRTEKVGKDTVLAQIISLVEEAQATKPPVQRIADVAVSYFIP
                     VVLLIAIGAFAAWYFLMGATLLFALTALISVLVVACPCALGLATPTAVTVGVGRGAEL
                     GILIRNGEALELADHLTTVLFDKTGTLTRGKPAVTDVVPADVDDLTLLSLAASVERNA
                     SHPLADAIVRKAGEMRVPIEQSGEFITFGGKGVAARVLGEEVLVGNRVLLAEKSVAIP
                     TEIDQQIIRLEEEGRTAVLVAVAGRVAGIIGIADTVKETTAEAIRDLTDMGLSVAMIT
                     GDNKRTAEAVGRQVGISRIIAEVLPQDKEREVKALQGAGDVVAFVGDGINDAPALARA
                     DVGIAIGSGTDVAIESGDIVLIKDDLIDAVAAIQLSRKVMSRIKQNIFWAFAYNSVLI
                     PLAAGVLYPFTGYTFSPVLAAFAMAASSVTVVTLSLLLKRYIPPAKKGKNQR"
     gene            1515..1664
                     /locus_tag="PK154_10265"
     CDS             1515..1664
                     /locus_tag="PK154_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPW11481.1"
                     /translation="MAIDPICKMTVDEKTAKFTSEFKGRTYYFCAPGCKKKFDTDPAK
                     YAEMA"
     gene            1777..>2103
                     /locus_tag="PK154_10270"
     CDS             1777..>2103
                     /locus_tag="PK154_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11482.1"
                     /translation="MSQEVFMARQEQVLAARIPPLLRLRKDLGLEGLVQGLDAVIINT
                     EPGRLNPAVSELLRSTGFTAGACFEDESYTTCVLKTRDSASLLVRARKKSENPFNTVN
                     AAGKTKK"
BASE COUNT          521 a          535 c          600 g          447 t
ORIGIN      
        1 gttgcgaccg gggatctcgt cattgtcaaa cccggcggaa agatcccggt cgatggagaa
       61 gtcaccgggg gagagagtta cgttgacgaa tcgatgataa ctggagaacc gatccctgtc
      121 ctgaagcaaa tagggtccaa ggtgattgga ggaaccctga atacgaacgg tatcttcacc
      181 ttcaggacag aaaaggtcgg caaagacacg gtgctcgccc agatcatcag ccttgttgag
      241 gaagcccagg ccacgaaacc gcctgtccag cggattgctg atgttgcggt ctcctatttt
      301 atccctgtcg tattgctcat agccatcggc gcatttgcag cctggtattt cctcatgggt
      361 gcaaccctcc tctttgctct tactgctctc atctctgtac ttgtggtggc ctgcccgtgc
      421 gcactcggcc tggctacgcc tactgccgtc accgttgggg tgggaagagg ggcagagctc
      481 ggtatactga tcagaaacgg tgaagcgctc gaactcgcag atcacctgac aaccgtacta
      541 tttgacaaga caggaaccct gacaagggga aaaccggcgg tgactgacgt ggtccctgcg
      601 gatgtagatg acctgaccct cctgtccctt gccgcgagtg tagagaggaa tgcatcccac
      661 cccctcgctg atgcgatagt caggaaagcc ggggagatgc gggtcccgat cgagcaatca
      721 ggggagttta tcacgtttgg ggggaaaggc gtggctgccc gggtccttgg agaggaagtc
      781 ctggtcggaa acagggtgct cctcgccgaa aaaagcgttg cgataccaac agaaatcgat
      841 cagcagatca tccgtctcga agaggagggg aggaccgccg tgcttgtcgc agtggccggc
      901 cgagtcgcag gcatcattgg aattgccgat accgtcaaag aaactactgc tgaggcaata
      961 cgggacctca ccgacatggg gctttccgtg gcgatgataa caggtgacaa caagaggacc
     1021 gcggaagcag tgggacgcca ggtgggaatc agccggatta ttgcagaagt gctccctcag
     1081 gacaaagaaa gggaggtaaa agcgctccag ggcgccggtg atgtggtcgc ctttgtcggg
     1141 gatggcatca atgatgcccc cgccctcgca cgggcagacg tagggatcgc gatcgggagc
     1201 gggacggatg tcgcgataga gagcggcgat atcgtgctga tcaaagacga cctcatcgat
     1261 gctgtcgctg ccatacaact ctcccggaaa gtgatgagcc gcatcaagca gaatatcttc
     1321 tgggcgtttg cctacaactc agtcctcatt ccgcttgctg cgggagttct gtacccgttt
     1381 accgggtaca cattcagccc ggtgcttgca gcgtttgcaa tggcagcgag ttctgtgacc
     1441 gtggtcaccc tctccctctt gttgaaaagg tatatacccc ctgcaaagaa agggaagaat
     1501 cagaggtgaa aggaatggcc atcgatccga tctgcaaaat gacggttgat gaaaagaccg
     1561 cgaaattcac gagtgaattc aaaggcagga cctactattt ctgtgcgccg ggatgtaaga
     1621 aaaagtttga tacagacccg gcaaagtatg ctgaaatggc ataaggaacc cctcttccat
     1681 ttttcccgtt tgccaacaat aatatgtgtc atatcccctt cattttctag aatcacaaga
     1741 tgatcccgaa aaagtacgat caataaagga gatgtgatgt cacaggaagt ttttatggcc
     1801 cggcaggagc aggttctcgc tgcccggatc ccccctcttc tcaggttgag aaaagacctg
     1861 ggacttgaag ggctcgtaca aggtcttgac gcggtcatca tcaatactga acccggacgg
     1921 ctgaatccgg ctgtctcaga acttcttcgc tctaccgggt ttactgccgg tgcctgtttc
     1981 gaggacgaat catacacgac ctgtgtgctg aaaacaaggg attctgcatc cctgcttgtc
     2041 agggcgagga aaaaaagtga aaatccgttc aatacggtga atgctgccgg aaaaaccaaa
     2101 aaa
//
LOCUS       DAOOCO010000251         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_56628_length_2082_cov_4.249137, whole genome shotgun sequence.
ACCESSION   DAOOCO010000251 DAOOCO010000000
VERSION     DAOOCO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56628_length_2082_cov_4.249137"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="ruvB"
                     /locus_tag="PK154_10275"
     CDS             complement(<1..599)
                     /gene="ruvB"
                     /locus_tag="PK154_10275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPW11483.1"
                     /translation="MREEALIAATMKEEDRDEEGIRPETLPEFIGQAQLRESLSIAIA
                     AAKKRGVPLDHVLFSGPPGLGKTTLAHIIAREMGSAIHCTSGPVLEKAGDLAAILTLV
                     QEGDLLFIDEIHRLPTVVEEILYPAMEDFRIDVMIGEGPSARSIRIDLAPFTLIGATT
                     RVGQLGSPFRDRFGMIFRLNAYSCEELAAIVRRSARILAL"
     gene            complement(596..1198)
                     /gene="ruvA"
                     /locus_tag="PK154_10280"
     CDS             complement(596..1198)
                     /gene="ruvA"
                     /locus_tag="PK154_10280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPW11484.1"
                     /translation="MISLLSGTVLWCGENTVVIDVHGVGFEVTMTDAAVRDLAGTVTP
                     VKIHTHLAVRDGEPLLYGFLTRSEVEMFLLLIQVNRVGPALALRILSQISIPDLAGAI
                     LDGDEAVLSKISGIGSKSAKRLILELGDKMKKRAALFVTGLPSGGDPVRRDAESALVA
                     LGFSHKEAVASVEAVLRGNSRSSDVAAVVRAALSLLREGS"
     gene            complement(1195..1677)
                     /gene="ruvC"
                     /locus_tag="PK154_10285"
     CDS             complement(1195..1677)
                     /gene="ruvC"
                     /locus_tag="PK154_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW11485.1"
                     /translation="MIVLGIDPGLATVGYGVLESRDGRVKTVLAYDCIRTSGKDSTTP
                     QRLEEIFNRVCSLISEHHPGWIALEKLFFSKNVTSALGVAEVRGVILLAAEQHRVKVA
                     EYTPNQVKLAITGSGRADKRQMQEMICRLLALPGIPKPDDVADGLCLALCHLHVVREP
                     "
BASE COUNT          435 a          642 c          597 g          408 t
ORIGIN      
        1 agtgccagga tccgtgcact cctcctgaca atcgctgcca gctcttcgca ggaatacgca
       61 ttcagccgga agatcatgcc gaaccggtcc cggaacggtg agccgagctg cccgacccgt
      121 gtcgtcgccc cgatcagggt gaacggggcc agatcgatcc gaatcgagcg ggcgctgggc
      181 ccctcaccga tcatcacatc gatccggaag tcctccatgg caggatacag gatctcttcc
      241 accacggtgg ggaggcggtg tatctcgtca atgaagagga gatccccttc ctggacgagg
      301 gtgaggatcg cagcaagatc cccggccttc tcaaggaccg ggccgctggt gcagtggata
      361 gctgacccca tctcccgggc aatgatatgg gcaagagtgg tcttcccgag acctggaggg
      421 cccgagaaga gcacatggtc gagcgggacc ccccgcttct ttgcggcagc aatggcaatc
      481 gacagcgatt ccctcagttg cgcttgcccg atgaattcgg ggagcgtttc aggcctgatc
      541 ccctcctcat cccggtcctc ctctttcatc gtggcagcga tgagcgcctc ttccctcatg
      601 atccctcccg gagaagagag agcgcggccc gcacgaccgc tgcaacgtca gaggatctgc
      661 tattcccgcg gagcacggcc tcaacagagg ccacagcctc cttgtgcgag aatcccaacg
      721 ctacaagggc actttccgca tccctccgga cgggatcacc gcctgatggg agaccagtca
      781 cgaacagcgc ggcccgcttc ttcatcttgt ccccaagctc caggatgaga cgctttgcac
      841 tcttggaccc gatcccggag atcttcgaga ggacagcctc atcaccgtca aggattgcac
      901 cggccagatc agggatgctg atctgcgaga ggattcgcaa ggcaagggca ggtcccaccc
      961 ggttgacctg gatcaggagg agaaacatct ccacctctga ccgggtgaga aacccataga
     1021 gcagaggttc accgtcacgg acggcaagat gggtatggat ctttacgggc gtgacggtgc
     1081 cggcaaggtc cctgaccgcc gcatccgtca tggtgacttc aaagcctacc ccatgcacat
     1141 cgatgaccac cgtgttctca ccgcaccaga ggaccgtgcc ggagaggagg gagatcatgg
     1201 ttccctcact acgtggagat ggcacagggc caagcagaga ccgtcagcca catcgtctgg
     1261 ttttggtatc ccggggaggg cgaggagacg gcagatcatc tcctgcatct gtcgcttgtc
     1321 tgccctcccc gaaccggtga tcgccagttt cacctggttt ggggtatact cggcgacctt
     1381 cacccggtgc tgctctgctg caagcaggat cacccccctc acttcggcaa cccccagcgc
     1441 tgaagtcacg ttctttgaaa aaaagagttt ctcgagggcg atccacccgg ggtgatgctc
     1501 cgatatcagg ctgcagaccc ggttgaaaat ctcttccagc cgctgggggg tcgtgctgtc
     1561 ctttcccgaa gtcctgatgc agtcatacgc aagaacggtc ttcaccctcc cgtccctgga
     1621 ctcaaggacg ccatatccca cggtggcgag acccggatcg atcccgagta cgatcatgtc
     1681 gccttcctgc attcgggctg caataagagt gattgtgcgt ttcaaaaaca tatccttttg
     1741 ccattcagga aaactggcgt tatgcgggca attctgcacg accggcaggt gctggagggc
     1801 ccgtgcacag cgcccggaac agtaccggga ggggcatgac ggcacacagg atggttgacg
     1861 ttccatgacg ggttcagttc ccatgacggg ttcagtgctc tcaattcact ccgtcggata
     1921 ttaccgccat tcccggatgg ggtgacaggg gaaatggcgt gaatgaacag ggctctccag
     1981 tgcggcatgg cacgtcagca tccctccatg ttccctctca atcgtgagac tgtcttgatc
     2041 ccactggcgg taaggaacat gaaaccttat aagaacggct at
//
LOCUS       DAOOCO010000252         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_57918_length_2053_cov_5.522523, whole genome shotgun sequence.
ACCESSION   DAOOCO010000252 DAOOCO010000000
VERSION     DAOOCO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57918_length_2053_cov_5.522523"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PK154_10290"
     CDS             <1..142
                     /locus_tag="PK154_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPW11486.1"
                     /translation="RLSKDGKIITSRGARTDIIMASVEAVVAGMNRLLREEHEDRSKN
                     PG"
     gene            114..1790
                     /gene="ilvB"
                     /locus_tag="PK154_10295"
     CDS             114..1790
                     /gene="ilvB"
                     /locus_tag="PK154_10295"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPW11487.1"
                     /translation="MKTGAKILVEGLQREGVETIFGYPGGVVLPIYDELYDSSLRHIL
                     VRHEQAAAHAADGFARASGRVGVCLATSGPGACNLVTGIATAYMDSVPIVAITGQVPT
                     SLLGNDAFQESDITGITLPITKHNYLVKRTEDLGRVLQEAFYIAGTGRKGPVLVDIPK
                     DVSMRSIEYDPAASEPVTLRGYQPTLRGHAKQIDKALEMISEAEQPVIYAGGGIIASD
                     ASEELVRLAETLVIPVTTTLMGLGAIPCDHPLNLGMLGMHGTRSANYAVTESDLLIAI
                     GARFDDRVTGKLDSFASHARIIHIDIDPAEIGKNKKVDVPIVGDVRSVLQDMLAKLQK
                     KKSYDRWLARIRGWKEKHPLQYVSDNCLRPQYVVEELSALLNGEGIIVSEVGQNQMWT
                     AQYFCFRKPRTWITSGGLGTMGYGFPAAIGAHFACPDQTVFDIAGDGSFQMNIQELST
                     VASNNIPVKVAILNNRFLGMVRQWQELFHNRRYSFTELPPVDFVKVANAYGVDGIKVE
                     SCEEVVPALKAAIETDGPFVLDFRVEREENVFPMVPAGAAINEMIGGKQE"
     gene            1787..>2053
                     /gene="ilvN"
                     /locus_tag="PK154_10300"
     CDS             1787..>2053
                     /gene="ilvN"
                     /locus_tag="PK154_10300"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPW11488.1"
                     /translation="MKPHTLSVLVENKAGVLSRVTGLFSRRGYNIESLAVGICEEPGM
                     SRITIVCIGDDAGIEQVMKQLNKLIDVIKVSDLTDNERVERELAL"
BASE COUNT          450 a          589 c          638 g          376 t
ORIGIN      
        1 aagattaagt aaagacggga agataattac gtctcgcggc gctcgcaccg acatcatcat
       61 ggctagcgtg gaagcggtgg tcgccggcat gaacagattg ttgagggaag aacatgaaga
      121 ccggagcaaa aatcctggtt gaagggctgc agcgcgaggg agtcgagacc atcttcggct
      181 accctggcgg tgtggtgttg ccgatatatg atgaactcta cgactcgtca ttgcggcaca
      241 tccttgtacg gcatgaacag gcagcagcac atgccgctga cggcttcgcc cgtgccagcg
      301 gccgtgttgg tgtatgcctt gccacctccg gcccgggtgc ctgcaacctg gtaaccggga
      361 tcgctacggc ctacatggat tcggtgccca tcgtcgccat taccggccag gtcccgacca
      421 gtctcctcgg gaacgacgcg ttccaggagt ccgacattac cgggatcacc ctcccgatca
      481 cgaagcacaa ctacctcgtg aagaggacgg aggatctcgg gagggtgctg caggaagcat
      541 tctacatcgc ggggactggt aggaaaggac cggtcctggt cgatatcccg aaagatgtga
      601 gcatgcgatc gatcgagtac gatcctgcgg cttctgaacc ggtgacgctc agggggtatc
      661 agccgacact cagaggccat gccaagcaga tcgacaaggc actcgagatg attagcgagg
      721 cagaacaacc cgtgatctac gcgggcgggg ggatcattgc atcggacgca tccgaagaac
      781 tggtgcgtct tgccgaaacc ctggtgatcc cggtcaccac caccctgatg ggactgggag
      841 cgataccctg cgaccacccg ctcaacctcg ggatgctcgg gatgcacggt acccgctcgg
      901 cgaactatgc cgtcaccgaa tcggacctgc tgattgcgat cggggcacgg ttcgatgacc
      961 gcgtcaccgg gaagctggac agcttcgcct cccatgcccg catcatccac atcgacatcg
     1021 atcctgcgga gatcgggaag aacaagaagg tcgatgtccc gattgtcggc gacgtgcgat
     1081 cggtgctgca ggatatgctc gcaaagctcc agaagaagaa gtcctatgat cggtggctcg
     1141 ccaggatcag gggatggaag gagaaacacc ccctccagta tgtctctgac aactgcctcc
     1201 gtccgcagta cgtggtcgag gagctctcag cgctcctgaa cggggagggc atcatcgtca
     1261 gcgaggtcgg ccagaaccag atgtggaccg cccagtactt ctgtttcagg aaaccccgga
     1321 cctggatcac ctccgggggg ctcggcacga tgggatacgg gttccctgcc gccatcggtg
     1381 cccacttcgc ctgccctgac cagaccgtct tcgatattgc cggggacggg agtttccaga
     1441 tgaatatcca ggaactctcg accgtagcct cgaacaacat ccctgtcaag gtcgcgatcc
     1501 tgaacaaccg gttcctcggg atggtgcggc agtggcagga actcttccat aaccggaggt
     1561 actcgtttac cgaacttccc ccggtcgatt tcgtgaaggt ggcaaacgcc tacggagttg
     1621 acggcataaa agtggagtcc tgcgaggagg tggtcccggc cctgaaagcg gccatcgaga
     1681 ccgatggccc gttcgtgctg gacttccggg tcgagcgcga ggagaacgtg ttcccgatgg
     1741 tccccgcagg ggcggccatc aacgagatga tcggggggaa gcaggaatga agccccacac
     1801 tctctcggtc ctcgtagaga acaaggccgg agtactctcg agggtcacgg ggctgttctc
     1861 acgccgcggt tacaacattg agagcctcgc ggtggggatc tgcgaggagc cgggcatgag
     1921 ccgtatcacc atcgtctgca tcggggacga tgccgggata gagcaggtga tgaaacagct
     1981 gaacaagctg atcgatgtca tcaaagtctc cgatctcacc gataatgaac gcgtggagcg
     2041 ggaacttgcc ctg
//
LOCUS       DAOOCO010000253         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_58059_length_2050_cov_3.968922, whole genome shotgun sequence.
ACCESSION   DAOOCO010000253 DAOOCO010000000
VERSION     DAOOCO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58059_length_2050_cov_3.968922"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1649
                     /locus_tag="PK154_10305"
     CDS             342..1649
                     /locus_tag="PK154_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HPW11489.1"
                     /translation="MTTKGILGRILLFLILSALIPGICAAAGSTYLSGTPDLSAVING
                     TNDFAPGSEQTLVIHVRNTGIDEIKIVQPGAGASGEPPSTARMVTLTLLPGDAPIMVK
                     TGTQMVGDIASGRSVFSPFSVKVLDDARGGTYHLPLLVNYTALVSEALIGEGSVTFQY
                     ATRTEQIDLPFNVKDEVIIAVDEITAPDLNAGGDGYITLTLRNTGTLWGNNTIARIFH
                     EEGSPLTPLDGRVYVGDFPPGGIATARFKVAVDEKAGPEQYPLVVAVEYQDASNATRL
                     SPQVSIGIPVVGKAQFSVIGTPVWMYRGDKKTIEVGYENTGPITAYSAQARISAADPF
                     DANDGTSFLGDLAPGDTAVARYTIGVDKAATVKEYGLDTEIRYRDSLDANRISDPMSV
                     KIQVRERGGLTRVLYNPVLMSIIIALLIGCGYFFFVHRKQKPE"
     gene            1844..>2050
                     /locus_tag="PK154_10310"
     CDS             1844..>2050
                     /locus_tag="PK154_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11490.1"
                     /translation="MVILFFWNGRLLQEKKEKVMNRSSDIREKKIRRISSVEPALFLR
                     YSSSSPWSTDNKETITRIGASRIGG"
BASE COUNT          454 a          602 c          566 g          428 t
ORIGIN      
        1 gtggtctatg acccggacaa gagtgccagc ccgtacggct tcatcgccac acaggacttt
       61 ggccggccgc tctaatccct cctttttcca gggacatgag catgaggccg gatcataagc
      121 cgccctcatg aaaccctggg atacggtttt tatgccagac tgcctccggc cgtttcctcc
      181 ctggtgcccg acaagacagc aggaagcgat ggattatacg aactatcccc ggtgtcttca
      241 ttggggctga ttctgtctta cgggaatcgt ggaatttttt tcggatccgg gaaccggaca
      301 agatatttat ccgagaaacg tgctacattg ttgttatcat aatgacaaca aagggaattt
      361 tgggccggat actccttttc ctcattcttt ctgccctgat tccggggata tgcgcagcgg
      421 caggcagcac ctacctctcc gggacccccg acctttccgc ggtcatcaat ggcaccaatg
      481 acttcgcacc cggttcggaa cagaccctcg tcatccatgt caggaacacc ggcatcgacg
      541 agatcaagat cgtccagccg ggagcgggtg cctccgggga acccccgagc actgcccgga
      601 tggtcaccct cacgctgctc ccgggcgatg ccccgatcat ggtaaagacc ggcacccaga
      661 tggtcggcga catcgcatcg gggcgatcgg tcttctcccc cttctcggtc aaggtgctcg
      721 atgacgcccg tggcgggacc tatcacctcc ccctgctcgt gaactacacc gctcttgtct
      781 ccgaggccct gatcggagag gggagcgtca ccttccagta tgcgaccagg accgagcaga
      841 tcgatctccc cttcaacgtg aaggacgagg tgatcatcgc ggtggatgag atcaccgccc
      901 cggacctgaa tgcaggcggt gacgggtata tcaccctcac cctgcggaat accggaaccc
      961 tttgggggaa caacacgatc gcacggatct tccacgaaga ggggagcccc ctgactcccc
     1021 ttgacgggag ggtgtatgtc ggggacttcc ctcccggggg gatcgcaacc gcccgcttca
     1081 aggtggcggt cgatgagaag gcagggcccg aacagtatcc cctggttgtc gcggttgaat
     1141 accaggacgc ctccaacgcg acccgcctct ctcctcaagt gagcatcgga ataccggtgg
     1201 tggggaaggc gcagttttcg gtgatcggga ccccggtctg gatgtaccgt ggcgacaaga
     1261 agaccatcga ggtcggctac gaaaataccg ggccgattac cgcatacagt gcccaggcca
     1321 ggatcagcgc cgcggacccc tttgatgcaa acgacggcac ttcattcctc ggcgatctcg
     1381 cacctggtga tacggccgtc gcccggtata cgatcggggt ggataaagcc gcgacggtca
     1441 aggagtacgg tcttgacacc gagattcggt accgtgacag cctcgatgcg aaccgcatct
     1501 ccgacccgat gagcgtcaaa atccaggtcc gtgagcgggg cgggctcacc cgggtcctgt
     1561 acaatccggt gctgatgagc atcatcatcg ccctcctcat cggctgcggg tacttcttct
     1621 tcgtgcaccg gaagcagaaa ccggaataat cggagagaag caaatgctct ccctgacaga
     1681 gcaacaccat ttttcgtgtg gaggaggccg gattatgtca cctggaaaaa tgccaccttc
     1741 agaagggaac tcccccaaga atttaagcct tcagggggtg ttgaccctcc ctgtaaagga
     1801 tattgactgc cacgtatgcg cagaatccgg tatatcctga tcgttggtta ttctcttctt
     1861 ttggaatggg agattgctgc aggaaaaaaa agagaaggta atgaacagat cctccgacat
     1921 tagagagaaa aaaataagaa ggatctcttc tgttgaacct gccttgttcc tccggtattc
     1981 ctcttcatca ccctggagca ctgataataa agaaaccatc actcgtatcg gtgcatccag
     2041 gataggtggt
//
LOCUS       DAOOCO010000254         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_59844_length_2012_cov_4.606541, whole genome shotgun sequence.
ACCESSION   DAOOCO010000254 DAOOCO010000000
VERSION     DAOOCO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59844_length_2012_cov_4.606541"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK154_10315"
     CDS             complement(<1..373)
                     /locus_tag="PK154_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HPW11491.1"
                     /translation="MVSVKKAAEQGNESLQIRMGAIQGSFSYKETAYHLPVTFALTGN
                     AVNSAKEAQQAYGASAHNPLVAAECLLASESTQRGEESPPFTGFIPDAVLRTLGYSLV
                     DGSILGLVLVTGTPPAPDTAAG"
     gene            578..859
                     /locus_tag="PK154_10320"
     CDS             578..859
                     /locus_tag="PK154_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPW11492.1"
                     /translation="MKMEHVPGKARGKITLYALSTCGWCAKTKELLRELGVDHFYVFV
                     DLLPPEELEEAIREVEKFNPAGSFPTLVINEGTRVIIGFREQEIREALG"
     gene            856..1368
                     /locus_tag="PK154_10325"
     CDS             856..1368
                     /locus_tag="PK154_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPW11493.1"
                     /translation="MSAAAPLTEEIQARYEKIKREAEEGGYHLNPDQAFTMNLVRGLL
                     VNEQRYGYPSCPCRISFGKREEDLDIICPCDYRDPDLDEFNACYCALYVSDAILKKEL
                     SVGSIPERRPPRSERKAALAGTSGRLTDMALPFPVWRCRVCGYLCARDAPPLICPVCK
                     ATKDRFERFI"
     gene            1435..1788
                     /locus_tag="PK154_10330"
     CDS             1435..1788
                     /locus_tag="PK154_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11494.1"
                     /translation="MASPIHIEIIGLKDLSCSPFPCDNTRSCGLYDCYPTGKLLVAFD
                     ALADEIGKEYGGRVVMTLTLIDDEVPPHVKAIIEEHYPPLPIVIVNGRYAPMGRISLP
                     MMQKEIEKCDREVRQ"
     gene            complement(1778..>2012)
                     /locus_tag="PK154_10335"
     CDS             complement(1778..>2012)
                     /locus_tag="PK154_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPW11495.1"
                     /translation="WLNNAIEAKNRVDAKIPAPKTPEAAKERSRMIEQENVRLQIEHL
                     KRYPVVKKALDEKKIEIHGLYYNLSDGSLTTIV"
BASE COUNT          449 a          523 c          574 g          466 t
ORIGIN      
        1 gcccggcggc agtatcaggt gcagggggtg ttccggtcac cagcaccagg ccaaggatac
       61 ttccgtcaac aagggaataa ccgagagttc ggagcactgc atccggaatg aacccggtaa
      121 agggagggga ctcttctccc cgctgtgtgg actcactggc aaggagacac tcggcggcaa
      181 caagcgggtt atgggcagat gcgccatagg cctgctgtgc ctctttggct gaattgacgg
      241 catttccggt aagggcaaag gtgactggga gatggtaggc agtctcctta taggagaatg
      301 atccctggat ggcacccata cggatctgta aggattcatt cccttgttct gctgcttttt
      361 tgacggaaac catgacatta cacccgggta attatcatac tctcgtggtt acaccctata
      421 tgggtttgga tcgcgccaaa tccggcaagg cgtctctttt tatcgaggta tatcagcctg
      481 cgtatcttgt cgtgccctcc cggtcttttg gcatttctcc tttgagagca tttaagagca
      541 gatccggcaa tcctatagca gggatctgat gaaccagatg aagatggagc atgttcccgg
      601 gaaagctcgg gggaagatca ccctctatgc cctgagcacc tgcggctggt gtgcaaagac
      661 gaaggagctc ctcagggaac tcggggtcga tcatttttat gtttttgtcg atctcctccc
      721 cccggaggag cttgaggaag cgatcaggga agtcgagaag ttcaatccgg ctggttcctt
      781 tccaaccctt gttatcaatg aagggacccg ggtcatcatc ggtttcaggg agcaggagat
      841 ccgggaggcc ctgggatgag tgcagcagct cccctgaccg aagagattca agcacggtac
      901 gaaaaaatca agcgggaggc agaagagggc ggctatcacc tcaatcctga ccaggcattc
      961 acgatgaacc tcgtcagggg acttctcgtc aatgagcagc ggtacggcta cccttcctgc
     1021 ccctgccgga tatcctttgg aaaacgcgag gaggacctgg atatcatctg cccctgtgat
     1081 taccgggatc ctgaccttga tgagttcaat gcctgttatt gcgccctcta tgtcagcgat
     1141 gctatcctga aaaaggaact ctctgtcgga tcaatccctg aacggaggcc gccaaggagc
     1201 gagaggaagg ctgctctggc cggaacatcc gggcgtctga ctgacatggc tctcccgttc
     1261 ccggtgtggc gatgcagggt atgcgggtat ctctgtgccc gcgatgctcc gccgctgatc
     1321 tgcccggtat gcaaagcgac aaaggatcgg ttcgagaggt tcatctgaag gtaagagata
     1381 cagcagggct gcaggatccc gaatgctaaa atatcccccg cagcagtacc tactatggca
     1441 tccccgatac atatcgagat catcggtctg aaagacctct cctgttcacc gtttccatgc
     1501 gataataccc ggagctgcgg gttatatgac tgttacccga ctggaaaact gctggtggct
     1561 tttgatgcac tcgccgatga gattggaaag gagtatggcg ggcgggtggt catgacgctg
     1621 accctgatcg acgatgaggt tccccctcac gtgaaagcga tcatagagga gcattaccct
     1681 ccgcttccca tcgtgatcgt gaacggcagg tacgctccca tggggaggat ctctcttccg
     1741 atgatgcaaa aagagataga gaagtgtgac cgggaggtca gacaatagtg gtcagcgatc
     1801 catccgaaag gttgtaataa agcccgtgaa tctctatctt tttctcatca agggcttttt
     1861 tcacgacagg ataccgcttc aggtgctcga tctggaggcg gacattctcc tgctcgatca
     1921 tccgggatcg ctcctttgct gcctcagggg tcttcggggc agggatcttc gcatccacac
     1981 ggttcttcgc ctcgatggcg ttgttcagcc ag
//
LOCUS       DAOOCO010000255         2006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_60143_length_2006_cov_4.409534, whole genome shotgun sequence.
ACCESSION   DAOOCO010000255 DAOOCO010000000
VERSION     DAOOCO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60143_length_2006_cov_4.409534"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK154_10340"
     CDS             complement(<1..341)
                     /locus_tag="PK154_10340"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HPW11496.1"
                     /translation="MFEKLSESSGNVVGYKVVGKLAPSDYEQLEPEFRALVEKEGWIR
                     LLFDMAGYKGETAKGWIADYKFGREFHNKVQKMAVVGDTTWEKWLTHLAKAVYATDAR
                     FFQSADISKAWT"
     gene            597..1649
                     /locus_tag="PK154_10345"
     CDS             597..1649
                     /locus_tag="PK154_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPW11497.1"
                     /translation="MKAAVYYSNHDIRIEEVGDLPVGPGEIKVRIMACGVCGSDVMEW
                     YRIKRAGRPGGIGAFGHECTGIITEAGPGADPKWREGDRVVVTHHVPCNTCNACLRRH
                     STACKTLQSTKFKHAFGAFAEYLVLPEINVDRGMVRLPESVSFDAGTFAEPLGCVIRG
                     QQWAPVSDGRSVLIIGAGITGLLHVQAALVNGAGYIAVSDMNLDRLQLARKFGADSTI
                     PATDDVPGRFRELNGGMGADTVIMTAPVPVCVQQALEAVGPGGTILFFAPTNPDIQSG
                     INLWDLWQNEVTITHSYAADLQNLSTALKWIQHDRINVADMITHVLPLKETAEGFLLT
                     AQPREGSLKVIVHPQE"
     gene            complement(1754..>2006)
                     /locus_tag="PK154_10350"
     CDS             complement(1754..>2006)
                     /locus_tag="PK154_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11498.1"
                     /translation="PDQGAYPHHANPVQARILVIGVNDPVSTRAVSTPAPQTNPGLFI
                     IGRNRFLPEGRYPGNLGAEWSYRNRSKHRWGYVSVPMTT"
BASE COUNT          446 a          545 c          535 g          480 t
ORIGIN      
        1 gtccaggcct tgctgatatc ggcggactgg aaaaacctgg catcggttgc atacacagct
       61 ttggccagat gcgttagcca cttctcccag gtagtatcgc ctacgaccgc cattttctgg
      121 actttattgt gaaattcacg cccgaatttg taatcggcta tccagccctt tgctgtttct
      181 cctttgtacc cggccatgtc aaacaacaaa cggatccaac cttctttttc taccaatgct
      241 ctgaattccg gttcaagctg ctcgtaatcg gatggcgcaa gctttccgac aactttgtac
      301 ccaacaacat tccctgaact ctcagatagt ttttcaaaca tttttctcct cttctacaaa
      361 ccgggacttg ctattatggc ataaaaggtt tgtggagaaa ggacgacata ctccctaaag
      421 ggagaggagg ttttaacatc cccccatccg gagaatagta ctgttccccc cctcatgatc
      481 accggggtat ttaaaaaaat tatcctggat accgtttcat tcctgtgacg ccagatcatt
      541 cctcgaaagc gagatttttc aaatctatta taccgttcct ctccacagtg aataccatga
      601 aagctgcagt ctattattcg aaccatgaca tccgcatcga agaggtgggg gatctccctg
      661 ttggaccagg ggagataaaa gtcaggatca tggcctgcgg tgtgtgcggg tcggatgtca
      721 tggaatggta ccggatcaaa cgtgccggca ggccgggcgg cataggggcg tttgggcacg
      781 aatgcaccgg tatcattacc gaggccggtc ctggcgcaga tccgaagtgg agggaaggcg
      841 atcgggtcgt ggtgactcac catgtcccct gtaacacatg caatgcctgc cttcgcaggc
      901 attcgactgc ctgtaagacc ctccaaagta ccaaatttaa gcatgcgttc ggggcgttcg
      961 cagaatacct ggtgctgccc gagatcaacg tggaccgtgg catggtgcgc ctgcccgaat
     1021 cggtctcgtt cgatgcaggg accttcgcag aaccccttgg atgtgtaatc cgtgggcagc
     1081 aatgggcccc ggtctcggat ggcaggtcag tattgatcat cggtgcggga atcaccggac
     1141 tgctgcatgt ccaggcagcc ctggtgaacg gtgcggggta tattgctgtc tcggacatga
     1201 accttgatcg gctgcagctt gccaggaaat tcggcgccga ttccacgatc cccgctaccg
     1261 acgatgttcc cggaaggttc agagaactga atggcgggat gggcgctgac accgtgatta
     1321 tgacggctcc ggttcccgtc tgtgtacaac aggcccttga ggcggtcgga ccggggggca
     1381 cgatcctctt ttttgccccc accaatcccg acatccagtc cgggatcaat ctctgggacc
     1441 tgtggcagaa cgaggttacc atcacccatt cctatgctgc ggatctccag aacctctcga
     1501 ctgccctgaa atggatccag catgacagga tcaacgtggc tgatatgatc acccacgtcc
     1561 ttcccctgaa agagacggct gaagggttcc tcctgactgc ccagccgcgg gaggggagtc
     1621 tgaaagtgat cgtgcatccg caggagtgac tcatttttct gttttaaccc ggggtcgaca
     1681 acagggcggt gattccattc tttttccaca tgcatcacgc ttgagatgag cgtggtggct
     1741 atcatcgaat cagtcacgtg gtcatgggta cggatacata tccccaccga tgttttgaac
     1801 gattccggta tgaccactca gcacccagat tcccgggata tcttccctct gggagaaacc
     1861 ggttacggcc tatgatgaaa agcccgggat tcgtctgtgg ggcaggagtg gagacggccc
     1921 gtgttgaaac cggatcattg acaccaatga ccagaatcct ggcctgaact ggattcgcgt
     1981 ggtggggata cgcaccctga tccggt
//
LOCUS       DAOOCO010000256         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_60970_length_1990_cov_3.076486, whole genome shotgun sequence.
ACCESSION   DAOOCO010000256 DAOOCO010000000
VERSION     DAOOCO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60970_length_1990_cov_3.076486"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PK154_10355"
     CDS             <1..166
                     /locus_tag="PK154_10355"
                     /inference="COORDINATES: protein motif:HMM:NF037139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW11499.1"
                     /translation="DTTEGFSGADLRIMVKEAFLSALIEGRSYITEGDINRGIILVSN
                     RGAVRHQNWL"
     gene            168..887
                     /locus_tag="PK154_10360"
     CDS             168..887
                     /locus_tag="PK154_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW11500.1"
                     /translation="MQITVLGTGDAIGTPKIGCSCPRCSSALHQGIQRLRTSFLIEHE
                     GKNLLIDSSPDLRQQLLAHGSPHIDAVLWTHGHYDHFMGFGEFYRVQAMPAVYAAPNV
                     LQYCGDIFRFLSFPRNTAEPYCPFDLFGLGLTLVPVNHPPVETYGIVLTDGRVKIAFT
                     SDSNASIPSESLSHFFGADLLFVDAMVPPSIHIPKHMNYHEACTLAAELAVKDFRCVH
                     LSHKIPWDLPHIGRDGETFHF"
     gene            1003..1272
                     /locus_tag="PK154_10365"
     CDS             1003..1272
                     /locus_tag="PK154_10365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HPW11501.1"
                     /translation="MNIKILELEKNKARLVISGEGHTFMNALTEEILTDPGVDVARYL
                     IKFQFSDPELLVTTKGDRDPLGAIKDACARLSAYCTDLLEKMPKQ"
     gene            complement(1289..1900)
                     /locus_tag="PK154_10370"
     CDS             complement(1289..1900)
                     /locus_tag="PK154_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HPW11502.1"
                     /translation="MVDPYEALLKKAYANVTEQSESTDRFVVPEAKVYIEGKTTILEN
                     FAEIADVVRRDQDHLMKYLLGELGTAGKIDGTRAIFNGKFEPSLINGLVKGYVDDYVI
                     CSECGKPDTRLVKDERVLLLRCDACGGHRPVRKRKARSEPVGSDLQEGQELEVEVQSV
                     SKRGDGVVRMGRYIMYIPRGKPGQKVKIRISRISGSIVFTEQI"
BASE COUNT          462 a          560 c          448 g          520 t
ORIGIN      
        1 cgataccacg gaaggattct ctggtgcaga tctccggatt atggtcaagg aagccttcct
       61 ctctgccctg atcgaaggga gatcctatat caccgagggc gatatcaacc gcgggatcat
      121 cctcgtcagc aaccggggcg cagtccgcca ccagaactgg ttataggatg cagatcactg
      181 tactgggaac cggggatgca atcggaactc caaagatagg gtgcagctgt ccccggtgtt
      241 cctctgcgct gcaccaggga atacagcggt tgcggacctc ttttttaatc gaacacgagg
      301 ggaaaaatct cctgatcgac tcttcacccg atctcagaca gcaactgctc gcccacggat
      361 ccccccatat cgatgcagtg ctctggacac atggccatta tgatcatttc atgggttttg
      421 gtgaattcta ccgggttcag gctatgcctg cagtctatgc agccccaaat gtccttcagt
      481 actgcgggga tatcttccgg ttcctcagtt ttccgcggaa cacggcggaa ccctactgcc
      541 cgttcgatct cttcggactt gggttgacgc tggtcccggt caaccatccc cctgttgaga
      601 cctatgggat cgtccttact gatggcagag taaaaatcgc attcacgtct gattccaatg
      661 ccagtatccc ttcagaaagc ctttcacact ttttcggtgc cgatcttctg tttgttgatg
      721 caatggtccc gccgtccatt catatcccga agcacatgaa ttatcatgag gcctgcaccc
      781 ttgcagctga actcgcggtg aaggatttca ggtgtgttca cctcagccac aaaatcccct
      841 gggatcttcc tcacattggc agagatggag agacgttcca tttttaacaa gtggcgtttt
      901 ttagggaggt ttccgggtca tgaatgattc ggccctgcac acgtcctcac tcgtggatct
      961 acacatacct atataccagt tcatctctca atgataggtg ggatgaatat caaaattctt
     1021 gagcttgaga agaacaaggc acgcctggtt atttctggag aaggccatac cttcatgaat
     1081 gctcttactg aagagatttt aacagacccc ggggttgatg tcgcacgata cctgataaaa
     1141 ttccagtttt cagaccctga actgctggtc actaccaaag gggaccgtga tccacttggt
     1201 gcgataaaag acgcttgtgc ccgcctttcc gcatattgca ccgatctgct tgaaaaaatg
     1261 ccgaaacaat aggtgcgatt taaaaaaatt atatctgttc tgtaaataca attgaacccg
     1321 atatacggga aatccgtatt tttaccttct gtcccggctt gcctcttggg atatacatga
     1381 tataccggcc catcctcacc acaccatctc cccgtttact gaccgactgg acttcaactt
     1441 caagttcctg tccttcctgg agatcgctcc ccaccggctc cgaacgggcc tttcttttcc
     1501 gcacgggacg gtgcccgcca caggcatcac agcgaaggag cagcaccctt tcatctttta
     1561 caaggcgggt atcgggcttt ccgcattctg aacagatgac atagtcatca acgtaccctt
     1621 tcacaagccc gttgatcagg gacggttcga acttcccgtt gaaaatcgct cttgtcccgt
     1681 ctatctttcc tgccgtgcca agctcgccaa gcaggtactt catgaggtga tcctgatccc
     1741 ttcgaaccac atcggcaatc tctgcgaaat tctcgaggat cgtcgttttt ccttctatgt
     1801 agacttttgc ctccggcacg acaaaccggt cagtggactc actctgctcg gtcacattcg
     1861 catatgcttt tttgaggagg gcctcgtacg gatcgaccat gatctatata ttcctcacaa
     1921 gacccctaaa tacccaacca tccccccctg ccgcgatgga aatttaaggt ttcaaaagag
     1981 agagctcctt
//
LOCUS       DAOOCO010000257         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_62087_length_1967_cov_3.350941, whole genome shotgun sequence.
ACCESSION   DAOOCO010000257 DAOOCO010000000
VERSION     DAOOCO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62087_length_1967_cov_3.350941"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PK154_10375"
     CDS             complement(<1..163)
                     /locus_tag="PK154_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPW11503.1"
                     /translation="MAMESVRGGRIFIAPNATVLGDVSLGDETGIWHGAVIRADKDTI
                     VIGNRSNIQD"
     gene            194..658
                     /locus_tag="PK154_10380"
     CDS             194..658
                     /locus_tag="PK154_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HPW11504.1"
                     /translation="MKVMVGGTFDPLHDGHKRLISRSFELAGKEGDVTIGLTTDQFAS
                     RKSHPVKEYAVRKTSLEQFIESSGFSTPYKIEPLNDRYGSAVSEDFDALVVSEETLPI
                     AHEINTFRHTLGKRKVDIHQITCVLAEDNRWISSTRIYRGEIDEHGNLLSRN"
     gene            complement(806..1417)
                     /locus_tag="PK154_10385"
     CDS             complement(806..1417)
                     /locus_tag="PK154_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW11505.1"
                     /translation="MLFSLVILSCGTTSADSLTAIDHYNRAVDYAYEGEYLLALNETD
                     RALAENPEFSLAHITRAGILNSLGRYEESLTASDQAIGLNPNQSGAWNNRAYALVHLG
                     RYEESLDASDRAISLDPDLTEAWINKGTALIALGRYNEALDASKNAAEIDPFSSDAKE
                     NKRIAEAALNTPPPTSASLPFLVILVAGLFGAGLMALTTKARI"
     gene            complement(1519..>1967)
                     /gene="pyrI"
                     /locus_tag="PK154_10390"
     CDS             complement(1519..>1967)
                     /gene="pyrI"
                     /locus_tag="PK154_10390"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPW11506.1"
                     /translation="EGLLISPIRDGTVIDHITGGEALNVLKIIGITGFTTESISVATN
                     VKSSKMGRKDIVKIENRELRKEEVDRIALIAPHATISIIRNFEVYEKKGVEIPRYLIG
                     VVRCPNPGCISNTNEPVPSQFEVSQKGLHCLYCDWMITNDIKNYIL"
BASE COUNT          495 a          470 c          463 g          539 t
ORIGIN      
        1 tatcctggat attggaccta ttgccgatta cgatagtatc cttgtcagcc ctgatcacgg
       61 caccatgcca gattcctgtc tcgtcaccaa gggaaacatc cccgaggacg gtggcatttg
      121 gtgcgataaa gatcctgccc ccacgcacac tctccatagc cataataaca tacgaagttt
      181 ataatctatc acaatgaagg ttatggtcgg gggaacattc gatccacttc atgatggtca
      241 taaacggctg attagccggt cgtttgagct tgccggaaaa gaaggtgacg tgactatcgg
      301 actcaccacc gatcagtttg cgagcaggaa atcacacccg gtgaaagaat atgctgtccg
      361 taaaacctca cttgaacaat tcattgaatc atccggattt tctacccctt acaagattga
      421 gcctctgaat gaccggtatg gatccgctgt ttcagaggac tttgatgccc ttgtagtgag
      481 cgaagagacc cttccgattg cccatgagat caatacattc cgccataccc tcgggaaacg
      541 gaaggtggat attcatcaga taacctgtgt tcttgccgag gacaaccgct ggatctcaag
      601 cacccgtatt taccgggggg agattgatga acacgggaat ctcctttccc gcaactaagc
      661 cagcgggaat gaaatgcgat cccgcaggat atccttgagg ttgaaggatt gaaccactcc
      721 aatctgatca atatttatac ctgctgttgg ggaacagata tctgatactc caagcatctc
      781 ctttttagta aaaatggtga ccggattata ttctggcctt ggttgtcaat gccatgagtc
      841 ctgctccgaa cagccctgca accagaatga ccaagaaggg tagtgatgct gaggttgggg
      901 gtggggtatt gagagctgcc tctgcaattc gtttgttctc ttttgcatct gaagaaaaag
      961 gatcaatttc tgcagcgttc ttggatgcat caagcgcttc gttatatcgg ccaagggcaa
     1021 tcagtgcggt tcctttattt atccatgcct cagtaaggtc aggatcgagt gagattgctc
     1081 ggtcagatgc atcgaggctc tcctcgtatc tcccaagatg taccagggca tatgctctgt
     1141 tgttccatgc tcctgactgg ttggggttca gcccgattgc ttgatctgat gcagtaaggg
     1201 attcttcata cctgcccagt gaattgagaa tccctgcacg ggtgatgtgt gcaagactga
     1261 attcaggatt ttctgcaagg gcacgatcag tctcgttcaa tgccaacagg tactctcctt
     1321 cgtatgcata gtcgaccgct ctgttgtaat gatcgattgc tgttaagcta tctgctgatg
     1381 ttgttccaca tgagagaatc accagtgaaa ataacatgat acttttgagg gctaaaagca
     1441 taaaagatga aaatcccgga ggcattttca ttggaaaaag gcagatactg ctacattttc
     1501 gttccattgt actcgcactc ataaaatgta gttcttgata tcatttgtga tcatccagtc
     1561 gcaatagagg caatggagcc ctttttgaga tacttcaaac tgggaaggga ctggctcgtt
     1621 ggtgttggag atacatccgg gattcgggca gcggacaacc ccaatcagat aacggggaat
     1681 ttctactcct ttcttctcat acacctcaaa attccggatg atgctaatcg tggcatgcgg
     1741 ggctatcagt gcgatacggt caacctcttc cttgcgaagt tcccggttct caatcttcac
     1801 gatatccttc ctgcccattt tactgctctt tacattggtt gcaacagata ttgactcggt
     1861 ggtgaatccg gttataccaa tgatcttgag gacattgagt gcctcccctc ccgtgatatg
     1921 atcaatcacc gtcccatccc ggatggggct gataagcaga ccttccc
//
LOCUS       DAOOCO010000258         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_65354_length_1907_cov_4.461663, whole genome shotgun sequence.
ACCESSION   DAOOCO010000258 DAOOCO010000000
VERSION     DAOOCO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65354_length_1907_cov_4.461663"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PK154_10395"
     CDS             <1..540
                     /locus_tag="PK154_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW11507.1"
                     /translation="EDTRCQERERPVPGFQELVGGPVRVSRLIGDGDSITLGDGRSLE
                     VIHTPGHSSGSISLLLSPEMILFSGDAIPVPGDFPIYDDPKASMRSLERLMNIERITY
                     LLSSWAEPKGGEEIYSSMDDGYAWVKEVSRAVSQITVAHPGIESSLLTRLVVKEIGLP
                     PEAANPMVMRTIMAHARKS"
     gene            696..1298
                     /locus_tag="PK154_10400"
     CDS             696..1298
                     /locus_tag="PK154_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11508.1"
                     /translation="MATTRMGYLVLVSIAILLLLSVVVVAIHPSLSIYTNGIRLAGLA
                     GFSLLAIAAIMTPWVREIHQTFGSSFLSIHHLFSITGLILITLHPVLLAVRLMNALVF
                     LPRLGSLQVVLSNAGRPSLILIYIALAGVMLRRVMPGYWRFFHALIWVALLLGIVHGN
                     LVGTDMKNPIVFVTFNSLMAVALGAFLLKRFKRRAPSKSE"
     gene            complement(1590..>1907)
                     /locus_tag="PK154_10405"
     CDS             complement(1590..>1907)
                     /locus_tag="PK154_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HPW11509.1"
                     /translation="AAIFFAIAIVIQSGVAGISIGITNALGGDWWSLVAAFIQLILGI
                     VLAIATIYLALNILDRLTKGINEFEELKKGNVAVALMMAGVIVATAVIIQSGVIGITS
                     ALI"
BASE COUNT          443 a          488 c          482 g          494 t
ORIGIN      
        1 gaagatacgc gatgtcagga acgggaacgt ccggttcccg gctttcaaga gcttgtcgga
       61 ggtccggtca gggtcagccg gctcatcgga gacggggatt cgatcacact tggtgatggc
      121 cggtccctcg aagttattca taccccgggc cattcatcag gatcaatctc gcttcttctc
      181 tctccggaga tgatcctgtt ttccggggat gctatcccgg ttccagggga tttccccatc
      241 tatgatgatc ccaaggcctc aatgcgatca cttgagcgtc tgatgaatat cgagcgtatt
      301 acctatctcc tctcctcatg ggccgaacca aaaggaggtg aagagatata ctcttcgatg
      361 gatgacggat atgcctgggt gaaagaggtg tcccgggcgg tcagccagat aaccgtcgcc
      421 catcccggaa tcgaatcctc gcttctcaca cggcttgtcg taaaggagat tggactacct
      481 ccggaggcgg caaacccgat ggtgatgcgg acgatcatgg cacatgcccg gaagtcctga
      541 gaggaatccc caaagcaatg ccctccaatg tggggacacc atgatagagt gaaccccttg
      601 aaatggattg cccggctcct gaatgcagct ggtcaggcca aattttgaga agtgaggaaa
      661 ctgctataag cccctgcgta aatcaggcct ttttgatggc caccacacgc atggggtatc
      721 ttgtcctggt cagtatcgca atccttcttc tattatctgt cgttgttgtt gcaatccatc
      781 cgtcactatc catttatacg aatggaatac gcctggcagg gcttgctggt ttctctttat
      841 tggcgatcgc tgcaatcatg actccctggg tacgtgagat acatcagaca ttcggcagtt
      901 catttctgtc gattcaccat cttttttcca taacaggcct catcctgatc actcttcacc
      961 cggttctgct ggcagtccgg ctgatgaatg ccttggtatt ccttccccgg ctgggttctc
     1021 ttcaggtagt tctttccaat gcagggcggc cttccctgat cctcatctat atcgctcttg
     1081 caggggtaat gcttcgtcgt gtaatgccgg gatactggcg gttcttccat gcccttatct
     1141 gggttgccct cctcctgggg atcgtccatg gcaacctggt tggtaccgat atgaaaaatc
     1201 caattgtctt tgtaaccttc aatagcctta tggcggttgc attgggggcg tttctcctca
     1261 aaagattcaa gcggagagca ccctctaaat cagaatgagc cgtctggttg aaggatacat
     1321 cagcatgttt ttcaaaaaga tcatgaacgg tgcgtgttct atcagtgaga tatgacccca
     1381 catttaccga tggagaataa ggaagctttc agatacggtt acaatcagtt gtaaataccg
     1441 ggttgtcagt gcgtctctct ctttccccat catgaaggta gtagtctgcg agtcatgggg
     1501 gagagctgat gtcaggaatg ctgttctcat gatctggcaa tatatggcac ttgttggact
     1561 accattccat cggaaaaaaa gaaaaatagt tagataaggg ccgaggtgat cccgataacc
     1621 ccggactgga tgatcactgc agtagcaaca atcactccag ccatcatcag cgcaacagca
     1681 acattgccct tcttcagttc ctcgaactca ttgatccctt tcgtcaggcg gtcaaggata
     1741 ttcagggcaa gatagatagt tgcgatggca aggacgatcc cgagaatcag ctggatgaag
     1801 gctgcgacaa gcgaccacca gtctcctccg agagcgttgg tgatccctat cgagattccg
     1861 gcaactccgg attggatcac aatggcgata gcaaagaaga tcgctgc
//
LOCUS       DAOOCO010000259         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_65537_length_1904_cov_3.454300, whole genome shotgun sequence.
ACCESSION   DAOOCO010000259 DAOOCO010000000
VERSION     DAOOCO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65537_length_1904_cov_3.454300"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK154_10410"
     CDS             complement(<1..406)
                     /locus_tag="PK154_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11510.1"
                     /translation="MEQRTFPILMGILLVLVILTAPAYAEIPPPEDSEDPQFLEVWDA
                     LIALQEQIADAFCTILALEDEVAGLKLENVWVSPLVAYPTGPNGPHTTYITSGICNAS
                     DLPCTPGALEITAETVVTEDDYQWVIFPLILPD"
     gene            complement(768..>1904)
                     /locus_tag="PK154_10415"
     CDS             complement(768..>1904)
                     /locus_tag="PK154_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW11511.1"
                     /translation="TKGNCAFPHYAVSEGSEISLEDIRFSVVETTGHTPESLCYVATD
                     LARGDSPVSLFSGDTLFVGDVGRPDLFPGRAEDLASSLYDNLHDKIMTLPDECEVYPA
                     HGAGSLCGRAMAAKRTSTIGYEKRYNYALRIKDKDAFVRALTSEMPAAPDHFTRCSAI
                     NRAGPALMKSLARPVPLGPGTFFEKMKDDRSLLLDVRSYQSFSGVHIPGAWHIDLSGN
                     FPTQAGWILPPEKDILLVVEEPRHADEATLQLHRVGFDRVAGYLDGGMLSWGAAGLPI
                     TRVPVLSADETHTLVSEGGAVLVDVRSSEEWDAAHAESSVNIPWHDLRTRFTELNPTL
                     HYILMCRGGQRASIAASILKMHGFGMVSNLGGGYTAYKRAGYAP"
BASE COUNT          432 a          527 c          542 g          403 t
ORIGIN      
        1 cgtcaggcag gatgagcggg aagatcaccc attggtaatc atcttcggtc accactgttt
       61 ccgcagtgat ttccagtgca ccaggagtgc agggaagatc tgacgcgttg cagatccctg
      121 aggtgatata ggtcgtatgc ggcccgttcg ggccagtggg gtatgccaca agcgggctta
      181 cccagacatt ctccaatttg agaccagcaa cttcatcttc gagggcaagg atagtgcaaa
      241 aagcatcggc aatctgctcc tggagcgcga tgagggcatc ccatacctcc aggaattggg
      301 gatcttctga atcttccgga ggggggatct cagcgtacgc aggtgctgtc agaataacga
      361 gtaccagcag tatccccata agtatgggga atgttctctg ttccattgat gccaccttgc
      421 tctttttcag agcaatgtga agattagagt aatactacat taatatattg tctcaaaaat
      481 aagccagcga acaggctcag tgcattgaaa aggaggcaga gaggccctgt tctcccgctc
      541 catcagacgc tatctccgta aaaaaccggg tcttgatggt cagccggagt gtacagaagt
      601 tgcggatacg ttcctttcag aaaagcagag agttgggatc tccccctcgc tctcaaaacc
      661 cgatgttccg caatccgcct aatgtgatcg ggaaattgcg ggaaatactg ttctcaatag
      721 gcacatcggg aatccggaag cccgccaccc ctcaagatcc cggcccgtca cggcgcatac
      781 ccggcccgct tgtacgcggt atatccgccc cccaggttcg agaccattcc aaacccatgc
      841 atcttcagga tacttgccgc gatgcttgcc cgctgcccgc ccctgcacat caggatataa
      901 tgaagtgtcg ggttgagctc ggtaaatctt gtccggaggt catgccacgg gatgttaacc
      961 gaggattctg catgggcggc atcccattcc tcgctggacc tgacatcgac aaggactgca
     1021 cccccttcac tgaccagcgt gtgtgtttca tccgcggaga ggaccggcac ccgtgtgatc
     1081 gggaggcctg cagcccccca tgagagcata ccgccatcaa gatacccggc gaccctgtca
     1141 aatcctactc ggtggagctg gagggttgcc tcatcggcat gcctcggttc ctcaacgaca
     1201 agcaggatat ccttttcggg gggaaggatc cagcctgcct gtgtggggaa gtttccggag
     1261 agatcgatgt gccatgcacc cgggatgtgg acccctgaga acgactggta actcctgaca
     1321 tcgaggagga gggaccggtc atccttcatc ttctcaaaga aggtacctgg tccgagcggg
     1381 acgggtctgg cgagggattt catgagggca ggccctgccc ggttgatcgc cgaacagcgg
     1441 gtgaaatgat cgggggcagc aggcatctct gacgtgagag ccctgacaaa tgcatccttg
     1501 tctttgatac ggagggcata gttgtaccgc ttctcatacc cgatcgtgct cgtccgcttt
     1561 gcggccatcg ccctcccgca gagcgatcct gccccgtggg cagggtagac ctcgcattcg
     1621 tcagggagcg tcatgatctt atcgtgaagg ttatcataaa gagaggacgc cagatcttct
     1681 gccctccccg ggaagagatc aggcctgcca acatcaccga caaacagggt atcgccggaa
     1741 aaaagggaga ccggggagtc gccccgggcc agatcggtag ccacgtaaca gagggattca
     1801 ggggtgtgcc cggtggtctc gaccacggag aaccggatat cctcgagaga tatctcggat
     1861 ccttcggaga ctgcgtaatg ggggaatgca cagttccctt tcgt
//
LOCUS       DAOOCO010000260         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_66327_length_1891_cov_4.280501, whole genome shotgun sequence.
ACCESSION   DAOOCO010000260 DAOOCO010000000
VERSION     DAOOCO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66327_length_1891_cov_4.280501"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1151)
                     /locus_tag="PK154_10420"
     CDS             complement(117..1151)
                     /locus_tag="PK154_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPW11512.1"
                     /translation="MEKRDTRRLKPEVQEELRKQAVRLRKNGMSYREIGEVLGVHPTS
                     ACTWYTTYLRDGLKAISMRRRGRRVGACRSLSAEQEKELKNLVIDKTPDQLKLPFALW
                     TREAVKEVVRDRYRIEMPIRTVGEYLKRWGFTAKKPVKRAYEQNPKAVQKWLQEDYPA
                     IAARAKSEGAEIHWADETGIESDTYNARGYAPKGTTPVIHLNAKKNHIGMISSISNHG
                     QVRFMMYREAMHADLLITFMKRLIKDAGKKVFLILDNLRVHHSKRVAAWLEEHKKEIE
                     VFYLPSYSPELNPDEYLNGDLKGRIRSGLPARSVKDLEKKTRSFMRTLVKRPHHVRNY
                     FRHPSVAYAA"
     gene            1535..>1891
                     /locus_tag="PK154_10425"
     CDS             1535..>1891
                     /locus_tag="PK154_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW11513.1"
                     /translation="MAKRRHDEIRRAVRENYGKVAQEGTTSCCCSPSSCCGKPGEAPL
                     TDISLGLGYSSKDFADVPDGSNLVLGCGNPQTIASLQPGETVLDLGSGGGFDCFLAAR
                     SVGDKGQVIGIDMTPEM"
BASE COUNT          414 a          537 c          447 g          493 t
ORIGIN      
        1 gagttcccga tcagacactg ttgagctcct taaactatat ctgaattttc cgattatatt
       61 actgaagact gctcaacaag tttattattg ctccggcaac taaacatgcg aacctttcat
      121 gctgcataag cgactgatgg atgcctgaag taattcctca catggtgtgg cctcttcacc
      181 aaggtcctca tgaaggagcg agtcttcttc tccagatcct tgactgaccg tgccggcagg
      241 ccagacctga tcctcccttt gagatcgcca ttgaggtact catcgggatt gagttccggc
      301 gaatacgatg gaagatagaa cacctcgatc tctttcttgt gctcctcgag ccatgcagcc
      361 actctcttgc tatggtggac ccgcagattg tccagaatga gaaacacctt cttccctgca
      421 tccttgatca gtctcttcat gaaagtaatc agcaggtcag cgtgcatggc ctcccggtac
      481 atcataaagc gtacctggcc atggttcgat atcgacgaga tcatgccaat atgattcttc
      541 ttcgcattca gatggatcac cggcgttgtg cccttgggcg catagccccg tgcattgtat
      601 gtgtcagact cgatgccggt ctcatcagcc cagtgaattt ccgcaccttc gctcttcgcc
      661 cgtgccgcta tcgcaggata gtcctcctgg agccacttct ggaccgcctt cgggttctgc
      721 tcgtaggccc gcttcaccgg cttcttcgcc gtgaaacccc acctcttcag gtactccccc
      781 accgtccgga tcggcatctc tatcctgtat cgatcccgga ccacctcctt gaccgcctcc
      841 ctcgtccaca gcgcaaaggg aagcttgagc tggtccggcg tcttgtcgat cacaagattc
      901 ttcagctcct tctcctgctc ggcgctcaac gatcggcacg ctccgactct ccggccccgc
      961 ctccgcatac tgatcgcctt caggccatct cgcagatagg tggtgtacca cgtgcaggcg
     1021 ctggtgggat gcaccccgag aacttctcct atctcccggt agctcatccc attcttacgc
     1081 aatcggaccg cctgcttcct cagttcctct tgtacctcag gcttcagcct ccgtgtgtct
     1141 ctcttctcca tgtcataatc ataccacaga tcatgttacc tttctatatt tatttgccgt
     1201 ggcaataaaa ggtggttgtg tatcttgaga ctgctggtaa ctaccccggc gaagaatgat
     1261 tatatgaata actgttcaac aacctgagac caatgcgaat gggttggact cttcggtttc
     1321 tttcgttctt aacaaccgtt tcttttttgt gtaagtaaag gttattccag gggcaggaga
     1381 accttcgatt tatcgatgca aacaacactc tgaaattgag aggatgttga ttacaaatat
     1441 acgtactcga ttctcatgat ggacatgaca aggtattgta tatctgcatt caggttacat
     1501 gatacgcata gatcactgac gagaaggaac catcatggcc aaacgcaggc acgatgaaat
     1561 acgcagagca gtccgcgaga actacgggaa ggttgcacaa gagggaacca ccagttgctg
     1621 ttgctcaccc tcatcttgtt gcgggaaacc cggggaagcc ccactgacgg atatttcgct
     1681 cggattggga tattcgagca aggattttgc cgatgtcccg gatggatcga acctggtgct
     1741 cgggtgtggg aacccccaaa ccatagcgtc cctccagccc ggtgaaacag tgctcgacct
     1801 cggcagcggc gggggcttcg actgtttcct tgcggcacgt tccgtaggtg ataaggggca
     1861 agtgataggc attgacatga cccctgagat g
//
LOCUS       DAOOCO010000261         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_66574_length_1887_cov_4.811135, whole genome shotgun sequence.
ACCESSION   DAOOCO010000261 DAOOCO010000000
VERSION     DAOOCO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66574_length_1887_cov_4.811135"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..706
                     /gene="uppS"
                     /locus_tag="PK154_10430"
     CDS             2..706
                     /gene="uppS"
                     /locus_tag="PK154_10430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPW11514.1"
                     /translation="MPHHVAIIQDGNRRFATSRGLIRKEGHRAGADRTEKVLEWSHDL
                     GIQHITLYSFSTENLSRDGEEVKDLFDLFKDRFNRVVTDERVHRDRIRVRMVGDRALL
                     PDDLISVITRAEEATREYDSYYLNVALAYGGRNEIVRAAREILEKVTSGHHTPEQIDA
                     ALVERHLCADHNLPPVDLVIRTGNECRTSNFLPWLANGHESAVYFCAPFWPLFRKIDL
                     LRAIRVYSQRRDALAA"
     gene            complement(722..1372)
                     /locus_tag="PK154_10435"
     CDS             complement(722..1372)
                     /locus_tag="PK154_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPW11515.1"
                     /translation="MIRRIYELILLRGIEVLPAHICFMITASDLQASPGKLTEVTSWC
                     LFVNGHFAGDKRNVTGDGGLPFPAIKGLTFHISTPDPVQIEPSLPVIRKVGEHARLHL
                     HIGETTETLGDGVDVCVAVGKSGREEIVSCIRKMAEVGESPESVSEQTFEKYLAFPSV
                     PDLVIKTGGSHLTDFLIWQSVYSELFFSDVNWDLFRKIDFLRALRDYQARVRKFGK"
     gene            complement(1369..>1887)
                     /locus_tag="PK154_10440"
     CDS             complement(1369..>1887)
                     /locus_tag="PK154_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPW11516.1"
                     /translation="LQNRSSSCRIIHGVEVDILADGTLGLPNRALGDLEIVIASIHSG
                     FSQPQDIITRRILAAIEHDHVDIIGHPTGRLIGKRQGYAVDMSRVIERARETGTALEC
                     NASPYRIDLDDGYIREAVEKGVRIAIGTDAHEKAELLHMEYGITTCRRGWASRQDILN
                     TLNGSGLLEWVE"
BASE COUNT          428 a          537 c          499 g          423 t
ORIGIN      
        1 tataccccac catgtggcaa tcatccagga cggaaacagg aggtttgcga catcacgcgg
       61 acttatccgg aaggaagggc atcgtgcggg agcagatcgc accgagaagg tgcttgaatg
      121 gtcccatgac ctggggatac agcacatcac gctgtattcg ttctcgacag agaacctctc
      181 ccgcgatggg gaagaggtaa aagatctgtt tgacctcttc aaggaccggt tcaatcgggt
      241 ggtaaccgat gaaagagttc accgggaccg gatccgggtc aggatggtcg gggacagggc
      301 gctcctccct gatgatctca tctctgtcat cacccgggca gaagaggcaa cccgggagta
      361 cgattcgtat tatctcaatg ttgctctggc ctatggcggc aggaacgaga ttgtgcgggc
      421 agcacgggag atcctggaaa aagtgaccag tggccatcac actccagaac agattgatgc
      481 cgcgctggtg gagcggcacc tctgtgcaga ccataatctc ccccccgtcg accttgtcat
      541 acgtaccgga aacgaatgcc gcacgtcaaa cttcctcccc tggcttgcaa acggccatga
      601 atctgcggtt tatttctgcg cccccttctg gccgctgttc aggaagatcg atctcctcag
      661 ggcgatccgg gtatattcgc aacggcgtga tgctcttgca gcctgaacag aaccccttct
      721 gttattttcc aaattttctg accctcgcct ggtaatcgcg gagtgcacgc agaaagtcaa
      781 tctttcggaa gaggtcccag ttgacatctg aaaagaacag ctctgagtag actgactgcc
      841 agatcaggaa gtcagtcagg tgagagcccc cggtctttat cacgaggtcc ggaacagagg
      901 ggaaagcgag gtatttttca aatgtctgct ctgacactga ttccggggat tcccccactt
      961 cggccatctt ccggatacac gagacgatct cctccctgcc gctcttcccc accgccacgc
     1021 agacgtctac accatctccg agcgtttcgg tagtctcacc aatgtggagg tgcaagcgtg
     1081 catgttcccc tactttccgt atgacgggaa gagagggctc tatctgcacg ggatcagggg
     1141 tgctgatatg gaacgtgagc ccctttattg ccggaaaagg aagacctcca tcaccggtaa
     1201 cgttccgctt atctcctgca aaatggccgt tgacaaacag gcaccagctc gtgacctcag
     1261 tcagtttccc gggtgaagcc tgcaggtctg atgcagttat catgaaacag atatgcgctg
     1321 gcaggacctc tattccgcgg agaagaatca attcgtatat ccggcgaatc attccaccca
     1381 ctccaggaga ccggatccat tcagggtatt gaggatgtcc tgcctcgatg cccatcccct
     1441 ccggcaggta gtaatgccgt actccatatg cagcagttca gccttttcat gggcatcggt
     1501 accgatcgca atcctgaccc ctttctccac cgcctcccgg atatatccgt catcaagatc
     1561 gatccggtac ggagatgcgt tgcattcaag agcagttccg gtctctctgg ctctttcaat
     1621 cactctgctc atgtcaaccg catacccctg ccgcttccca atgagccgtc cggtcgggtg
     1681 cccgatgata tccacgtggt catgctcaat ggcggcgagg atgcgtcgcg tgatgatatc
     1741 ctgtggctgt gaaaatcctg aatgaattga tgcaatcact atctccaggt caccaagggc
     1801 ccggtttggc agccccaggg tgccatcagc gaggatatcg acctctactc catggatgat
     1861 acggcaggaa gaagagcggt tctgaag
//
LOCUS       DAOOCO010000262         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_66988_length_1879_cov_3.741776, whole genome shotgun sequence.
ACCESSION   DAOOCO010000262 DAOOCO010000000
VERSION     DAOOCO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66988_length_1879_cov_3.741776"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK154_10445"
     CDS             <1..792
                     /locus_tag="PK154_10445"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPW11517.1"
                     /translation="QSEKSPAHDINQRRDWQVTSLIDVMGSMEALGTSGIPLVAAFFI
                     GLMMTFSPCPLATNITAIAFISKKVGDSKHTLLVGTLYALGRMAAYIGITALIVYAGL
                     NIQAISLFLQEYGEKIIGPFLVIMGILLLEIVEVPLPGGHGWLQKIEIYLGEKGYLGG
                     FLLGVIFALALCPFAAVLFFGMLIPIALKTGDALIVPAVFAIATALPVIIISLALVSG
                     VNRVSGMMKTVQKYEKWIKWAVAAVFIVVGIYYIVIVYGPMIGIG"
     gene            1391..>1879
                     /locus_tag="PK154_10450"
     CDS             1391..>1879
                     /locus_tag="PK154_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW11518.1"
                     /translation="MVLKKRQTGCITLALAGILVLGLVATGCVGNEPTSSSSQERTSI
                     KVTGSTTVLPIAQLAADAYMGTHSDADIQVTGGGSSVGVQAAGEKTADIGMSSRDVKS
                     DELKKYPNLVITTIGKDGVALILHPSNTITSLTTTQIKDIYAGNYTNWNQVGGPDLAI
                     VVV"
BASE COUNT          478 a          439 c          458 g          504 t
ORIGIN      
        1 caatcagaga agtcacctgc ccatgacatc aatcagagaa gagactggca ggtgacttct
       61 ctgattgatg tcatgggcag catggaggcg ctggggacca gcggaatccc cctcgtcgct
      121 gcattcttca ttggcctgat gatgaccttc tccccctgtc ccctcgccac caatatcaca
      181 gcaattgcat tcatatcaaa gaaggttggc gacagtaagc ataccctcct cgtaggaacc
      241 ctctatgcac ttggaaggat ggcggcttat atcggcatta ctgccctgat cgtgtatgca
      301 gggctgaata tccaggccat ctctctgttc cttcaggagt atggagagaa gataatcgga
      361 ccattccttg tcatcatggg tatcctgctg ctggaaatag ttgaagtacc gcttcctggg
      421 ggtcacggat ggttgcagaa gatcgagatc tatcttgggg aaaagggata tctcggggga
      481 ttcttgcttg gtgtgatctt tgccctcgcc ctatgtcctt ttgctgcggt acttttcttc
      541 ggcatgctca tcccgatagc cttgaagacc ggtgatgctc tcatcgttcc tgcagtcttt
      601 gccattgcta cggcactccc cgtaatcatc atctctcttg ctctcgtgag tggggtgaac
      661 cgggtatctg ggatgatgaa aacggtgcag aagtacgaaa agtggattaa gtgggctgtt
      721 gccgcggtgt tcattgttgt gggaatatac tatatcgtca ttgtatacgg gccgatgatt
      781 ggaataggat aaggtcattc tctttctctc cacatcaaac caatgagcac ctctgaagtg
      841 aattcgatca ataccgaatc ggtttatgcc aggagtgaag gattgtccac gtttgacgga
      901 cctgactacc tgcaatgaac tctggggaag gtgcggagaa agggttcggg ggaggtacat
      961 aaatatgcgc ggacgccaat cggggcgcat taaacgctca gttctacctc ccacccgcag
     1021 catcatgttc ttttttcgta tttcaacttt ggaaaagaaa gtaaaggaat caattctcaa
     1081 cacatagctg gctgactcta tgattttcat agactaagga agaatctttc caagaggagt
     1141 taaaaaatgg tataagactt aaaaatttga tttgcagcat ttgctgatca tctcagttca
     1201 caatagatgt acaatttcag atagctgaaa ccttccttgg gtcttttaaa acctggattg
     1261 tgaagtttac ctcacgtcgt gtatacgcga gtcgccgatt ctctatatcc atccgattga
     1321 tatatagaag actatattct atgtcgaaat gttatatatt catttaaatc cgttttgtat
     1381 tcagaccacc atggttctga aaaagagaca aactggatgt atcaccctcg cactcgcagg
     1441 aattcttgtt ctgggacttg tcgcgaccgg gtgtgtggga aacgagccaa catcgtcctc
     1501 ctcacaggaa cgaacatcga tcaaagtgac gggctcaaca acggtccttc ctatcgccca
     1561 gctggctgct gatgcgtata tgggaaccca ctccgatgcc gatatccagg ttactggagg
     1621 aggctccagt gtcggggtcc aggcagcagg ggagaaaact gctgatatcg ggatgtcctc
     1681 acgtgatgtc aagtcagatg aactgaaaaa atatccgaac ctggtgatca ccacgattgg
     1741 gaaagacgga gttgcgctta ttctccaccc gtcaaacacc attaccagcc tcaccacgac
     1801 ccaaatcaag gatatttatg ctggtaatta tacaaactgg aaccaggttg gcggccctga
     1861 tctggcgatt gtcgttgta
//
LOCUS       DAOOCO010000263         1855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_68409_length_1855_cov_4.561111, whole genome shotgun sequence.
ACCESSION   DAOOCO010000263 DAOOCO010000000
VERSION     DAOOCO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68409_length_1855_cov_4.561111"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..456)
                     /locus_tag="PK154_10455"
     CDS             complement(130..456)
                     /locus_tag="PK154_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11519.1"
                     /translation="MSVDEVLDLQPGELVQVRSLDEIYSTLDGEGKYKGLLFMPEMED
                     FCEKKYHVFKKVRSITLETDGEVRKLKSPTVFLEGVYCDGKRHNNCDRSCLLFWREAW
                     LKRVEP"
     gene            1175..>1855
                     /locus_tag="PK154_10460"
     CDS             1175..>1855
                     /locus_tag="PK154_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPW11520.1"
                     /translation="MGYSIQELSEENAGDWDEFNNRCPEGTFYHTVKWKNLLENTLNI
                     DLKYWIIWKGQKMVGILPFMNRPLVFLKGLEPIPHSEFNNILLKDDLDLRDLDKVLTL
                     FASKYSFLSLNMDSKLLWDHMGYNHHSSENTGNMVLDLRQNPPDKIWEDFTARKGQRR
                     FIRRFGEKGFWIREIHEQEDMKTFYRYYAENLARINGDILPLAFFQSLLEAYSQNGMR
                     VTLLSKEDI"
BASE COUNT          478 a          484 c          454 g          439 t
ORIGIN      
        1 tgaaccgtca accttcgcac ccattaaatt gctgccctgt gccccgcaag gggccggccc
       61 cggcagcaag ccccacccaa aaggcgcggg gtagttgaca tgctccgagt gcaggagtaa
      121 tgaggtgggt tagggttcga cccgcttcaa ccaggcttcc ctccagaaga gcaggcagga
      181 tctgtcacaa ttattgtgcc tcttcccatc gcagtatact ccctccagga agaccgtagg
      241 acttttcaac tttctcacct ccccatcggt ttcaagggtt attgagcgca ccttcttgaa
      301 cacatggtat tttttctcac aaaagtcttc catctctggc ataaagagca ggcccttgta
      361 tttcccctct ccgtcaagcg tggagtatat ctcatctaac gatcggacct ggactaattc
      421 accgggttgc agatcaagca cttcatccac agacataacc cccctttgag ccatttttag
      481 ttgattcgta tcacgagtgc catcatcggc aggcgagatc atcagctgaa tctggcgcca
      541 caatgatacg gtatagggga tccgcttgat gataggtatc aaaggggcct tgatagaatc
      601 attccaggtc ctctgcacga ggtatccata cgacggtcgc gtaaattcac atacctcccg
      661 ggccatgaat gtacagggta ttatgtgctt ctcctccata cttccctctc ccttatccac
      721 cactaaaccg gatctcatac acctctgacc ggcattcgcg cattccaatg ttcctcctgt
      781 aattatcagg agctctgccg ggacgtttca tgcagtgtcc cgggaaaggt atagggtcgg
      841 ccacactctt tccctctcac ccgatcgggc aaaccggtag agaccctgga caggccgggt
      901 tcaggatgtc ataatacata aaccatttgc tgcaggcacc gaagagatcg catgatcccg
      961 ctccgcctgg gtagtatcca cctggatagt gcagcaaccc acagagggga gatgtgggga
     1021 gatccagacc ccggttccat gcggtgcccc tcaccggtgt tcggggcgac cctggtgttg
     1081 cccggaaagg agggtaaaac catccgttac tgataagaac cgcagtgtat cgagccggaa
     1141 gttacattac atcaggggaa agtagtaagc catcatgggc tactccatac aagagctgtc
     1201 ggaggagaat gcaggagatt gggacgaatt taacaaccgg tgcccggaag gaacgtttta
     1261 ccatactgta aaatggaaaa acctgctcga gaatacactc aacatcgatc tcaagtactg
     1321 gatcatctgg aaagggcaga aaatggtcgg gatactcccc ttcatgaatc gcccattagt
     1381 attcctgaaa gggcttgagc ctatacctca ttcagagttc aataacatct tgctgaagga
     1441 tgatctcgat ctccgggacc tggataaggt tctcaccctg ttcgcatcaa agtactcatt
     1501 tctctccctg aatatggata gtaagttatt atgggaccac atgggataca accaccattc
     1561 ctctgagaat acagggaata tggtgctgga tttacggcaa aatccgccgg ataagatctg
     1621 ggaagatttc accgccagaa aagggcagag gagatttatc cgcaggttcg gtgagaaggg
     1681 gttttggatt cgcgagatcc acgagcagga ggacatgaaa acattttaca ggtattatgc
     1741 tgaaaacctc gcacgcataa acggagatat cctgccgctc gccttctttc agagtctgct
     1801 agaagcatat tcacagaatg ggatgcgggt gacgttgtta tcgaaagagg atata
//
LOCUS       DAOOCO010000264         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_68499_length_1854_cov_2.821568, whole genome shotgun sequence.
ACCESSION   DAOOCO010000264 DAOOCO010000000
VERSION     DAOOCO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68499_length_1854_cov_2.821568"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /gene="ppk1"
                     /locus_tag="PK154_10465"
     CDS             <1..1843
                     /gene="ppk1"
                     /locus_tag="PK154_10465"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPW11521.1"
                     /translation="EPMLARQSDLWREEMVPALRDAGIHILSYGEISGAEKALLRDYF
                     QREVYPVLTPLAFNPSHPFPFISNLSLNLAAILKHPSSRTEHFARVKVPTNLFPRLIQ
                     VPGESRGSDYKGDTCRVFLEELIGANLDLLFPGYQVEGVFPFRVTRDADLEIEEDEAS
                     DLLTVVEEGIGMRRVGSPVRVEVAEGMPSSACSIIGRKLGIAAEMFYQVRAPLGIADL
                     MELSGLDRPDLKDLPFVPSVPPGLGEQDAIFSRIREKDEPLFHPYESFTPVISLLRQA
                     ANDPAVLAIKITLYRVGRHSPVVDALLEARENGKVVAALIELKARFDEENNIGWARAL
                     GRAGVHVVYGLPGLKVHSKICMVVRREQDGLRTYVHLGTGNYNATSSRIYTDFGLFTA
                     DPAIAADVTDLFNVLTGCATPRSYRKLLVAPQTLREGILSRIERERKRHREHGDGCII
                     FKMNALVDRDCILALYRASAEGVRVLLQVRGICCLRPGVAGVSETIQVTSIVGRFLEH
                     ARLYYFRNGGEDEVLLGSADLMPRNLDRRIEVLFPVEDRALASWLMKTIVPVHLDDTV
                     KNRILHPDGTYSHIVPQPGQPALDAQEWCIAHRAAWSEQKRSETRRG"
BASE COUNT          374 a          544 c          560 g          376 t
ORIGIN      
        1 tgaaccgatg cttgcccggc agtcggatct ctggagggag gagatggtcc ctgctttgcg
       61 ggatgcaggg atccatatcc tgtcgtatgg ggagatatca ggtgcggaga aggcgctgct
      121 ccgggattac tttcagcggg aggtgtaccc ggtcctgaca cccctcgcat tcaacccttc
      181 acatccgttc cccttcatct cgaatctctc gctcaacctg gcggcaatcc tgaaacaccc
      241 ctcctccagg acagagcatt ttgcccgggt caaggtcccg acaaacctgt ttccccggct
      301 tatccaggtc cctggtgaga gcagggggag tgactataag ggggatacct gccgggtctt
      361 cctcgaggaa ctcattggcg caaacctcga tctcctcttt cccggctacc aggtggaagg
      421 tgtgtttccc ttcagggtaa cccgtgatgc agaccttgag atcgaagagg atgaggcatc
      481 cgaccttctc accgtggtag aagaggggat cggaatgcgg cgggtgggct ccccggtccg
      541 cgtcgaggtg gcagaaggga tgccctcatc ggcctgcagc atcatcggca ggaaactcgg
      601 tattgctgcg gagatgtttt accaggtccg tgccccgctc gggatagcgg acctcatgga
      661 actttccggg ctggaccggc ccgacctgaa agacctcccg ttcgtcccgt cagtcccccc
      721 cggtcttggc gaacaggacg cgatcttctc ccgtatcagg gagaaggacg aacccctgtt
      781 tcatccgtat gagagtttta ccccggtcat cagcctgctc cgccaggcag ccaatgatcc
      841 tgcggtactg gcaatcaaga taaccctcta ccgggtcggc agacactccc cggttgtgga
      901 tgccctgctc gaagcgaggg agaacgggaa ggttgtggca gccttgatcg agctcaaagc
      961 caggtttgac gaggagaaca atatcggatg ggcccgggcg cttgggcggg caggggttca
     1021 cgtggtatac ggccttcccg ggctcaaggt gcattccaag atctgcatgg tggtcaggcg
     1081 tgaacaggac ggtctccgga cctatgtgca cctgggtacc ggaaattaca atgccacctc
     1141 cagcaggatt tacaccgatt tcggattatt caccgcagat ccggcaatcg cagccgatgt
     1201 cactgacctt ttcaacgttc tcaccgggtg tgcaacccct cgctcgtacc ggaaactgct
     1261 cgtggcgcct caaaccctcc gggaggggat tctctcccgt atcgagcggg agcgcaagcg
     1321 gcaccgggag catggtgacg gctgcattat cttcaagatg aatgccctcg tcgaccggga
     1381 ctgtatcctt gccctgtacc gggcatcagc ggaaggagtc cgggtcctcc tccaggtgcg
     1441 gggcatctgc tgcctgcgcc cgggtgtggc cggggtgagt gaaacgatac aggtgacttc
     1501 aatcgtcggc cggttcctcg aacacgcccg tctttactac ttcaggaacg gtggagagga
     1561 tgaagtgctc ctcggaagcg cggacctcat gccccggaac ctcgaccgga gaatcgaggt
     1621 gctgttcccg gttgaagacc gggcgctcgc atcctggctg atgaagacca ttgtcccggt
     1681 gcatctcgat gataccgtga agaacaggat tcttcacccg gatgggacct attctcatat
     1741 cgtccctcaa ccgggccagc ctgccctgga cgcacaggaa tggtgcattg cccaccgggc
     1801 agcgtggagc gaacaaaaaa ggagcgaaac cagaaggggg tgaaaaccat aagg
//
LOCUS       DAOOCO010000265         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_68732_length_1850_cov_3.269638, whole genome shotgun sequence.
ACCESSION   DAOOCO010000265 DAOOCO010000000
VERSION     DAOOCO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68732_length_1850_cov_3.269638"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..562
                     /locus_tag="PK154_10470"
     CDS             203..562
                     /locus_tag="PK154_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11522.1"
                     /translation="MTDITRKKIFLAVQDIRTLAARTRPDCEGSLECTDCPARIETGD
                     RFDFECLFSLCETMQERGWRKKGNVSSHDRSPARPSHRRGTFKRRPAPMRDPIMRGRH
                     PMDFRTSGDALLPGSER"
     gene            840..992
                     /locus_tag="PK154_10475"
     CDS             840..992
                     /locus_tag="PK154_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11523.1"
                     /translation="MTRRTKGKRTHRTCDCCGREIFSYQDTVYGDRRTYHYECWKPPI
                     TNDTCR"
     gene            complement(1339..1656)
                     /locus_tag="PK154_10480"
     CDS             complement(1339..1656)
                     /locus_tag="PK154_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11524.1"
                     /translation="MNDKGYGRDFRRYPVSGLIRPTRKEWISAVDEFVILPGKISRAF
                     SRIRFIVIDNDGREIRREGGSGSGAVMEPMNSRVLNERLSFSYYGICAPCPLTERIPG
                     IME"
BASE COUNT          432 a          555 c          475 g          388 t
ORIGIN      
        1 cggacgaccc tgtactattc cgccgaccag gtactctggg gactgatcat cctggccgtg
       61 ctcgtattcc tgttattgat cgtcgggagg cggctggtat ggtgatactg gtcgccccca
      121 aggggcggga ggttgccctc gatcaccacc agcatgccgg atgttccccc cagcccggca
      181 tagacacgga cgtgagacag gcatgaccga tatcaccagg aaaaagatct tcctcgcggt
      241 gcaggatatc cggactcttg cggccaggac ccgccctgac tgcgagggca gcctcgaatg
      301 caccgactgt ccggcacgga tcgagaccgg ggatcggttc gacttcgaat gcctcttcag
      361 cctctgcgaa accatgcagg aacggggatg gagaaagaag gggaatgtgt catctcacga
      421 cagatcaccc gcccgtccat cccatcgcag gggcaccttt aaaagaagac cggcgccgat
      481 gcgggatcct atcatgcgtg ggagacatcc catggatttt cggacatcag gagatgcact
      541 cctgccagga tcggaacggt gaacggggga aatatcccct gttgtggcag ggctcggtcc
      601 ctgacaccat gagtgatgac atcaatgtac cttcactctg gcgggatcgg tggttaccat
      661 ccccatgcga taccaaaacg gaacaagcca cgaggatgag ggaggattgc cactctcact
      721 cattgctcct cctggcaatg aaaagaagcg ccgtggaact gcgggaagat caaagaccat
      781 cggtccatct tctgacaggg agtactcaaa aggtgcaggg tatctcgtgg aggaggaata
      841 tgacacggag aacgaaggga aaacgcaccc ataggacctg cgactgttgt gggcgggaga
      901 tcttcagcta ccaggacacg gtctatggtg atcgccggac ctatcactac gaatgctgga
      961 agccccccat caccaacgat acctgcaggt gatcccgtgg aacggtgctc tctcccatgc
     1021 cgatcggcat ccactacctc ccgggcgaac ccgatgaccg tcctcattcc ctgaaccccg
     1081 aggttcaaat aatttaaata ccctgagaag aaccccctat ctggcaatga tttggaaatt
     1141 catcgtgaat ctggtggcat aggcgttcaa ggtggcaaca ataaactcca gcattcgtcc
     1201 ggacaaccag ggtcgtggtg aaccttcctt ggccggggat cttccccggc cgtctttgca
     1261 ctaccattga atgggatatc ctgtcctgat attccatcct ccctcccgac atgtccaaac
     1321 catacccctt tccctttttt actccatgat tccaggaatc ctctctgtca aaggacaggg
     1381 ggcacaaatg ccgtaatacg agaacgacaa tcgttcattg agcaccctgc tattcatcgg
     1441 ttccatgaca gcacccgacc cggaaccacc ttctctccgg atttcgcgac catcgttgtc
     1501 gattactatg aagcgaatcc ggctgaatgc cctgctgatt ttgcccggca agatgacgaa
     1561 ttcgtcaacc gcactgatcc attccttcct ggtgggcctg ataagccctg aaacaggata
     1621 tctccgaaaa tccctgccgt aacctttatc attcaccgtt ccgtttctct actcatcggg
     1681 aaagcagggc tcgtccctgc atcccctgga gatacgtgcc atgaccccct cctcttcgtc
     1741 caggatcagc aaggtcaggt atcaccggat ctacgcagat acccagggag actcgcactt
     1801 tgcgaccgat atcatcgaac agagcagtgt ccaggcagcc ccccctgccg
//
LOCUS       DAOOCO010000266         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_68837_length_1848_cov_5.307864, whole genome shotgun sequence.
ACCESSION   DAOOCO010000266 DAOOCO010000000
VERSION     DAOOCO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68837_length_1848_cov_5.307864"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK154_10485"
     CDS             complement(<1..969)
                     /locus_tag="PK154_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11525.1"
                     /translation="MMIAVEGVSLIRGDFSFARISLQVPESSYTVLMGPSGAGKTLLL
                     ESIGGFLQVTDGRILVAGRDVTILPPEKRGIGFMYQDYSLFPHLTVEENIAYGLKMKG
                     IASRERADTVNTLLSSFSLHPLAHRHTGSLSGGEKQRVALARALATRPSALLLDEPFA
                     ALDPEARLSCMDEVKALQRENGLTILQVSHDCDEAYRLADQVVVMDKGRILQAGTPAA
                     VFSRPVHPTIAALAGFENMLEGTSGAFESGRTLLSIGTAGIIAEGMVPAGERILACIR
                     SRDILLAGADISRSEQVNRIEGQVISMVATDHGYQVRFAGPFPLLID"
     gene            complement(953..1786)
                     /locus_tag="PK154_10490"
     CDS             complement(953..1786)
                     /locus_tag="PK154_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11526.1"
                     /translation="MSSPEHSLKKNHWSSGHSLAAFWIPGMILVGIIALALLVIAVPV
                     FQNPEYLAGAASDPMVFQAFFVTFLAGFCAVGILAITGTPLAYALSRSDTPWKGIVET
                     LVDIPLVLPHTVAGLMVYLLFMSRGLLGAPLGGIGLTFEDAFPGIVAAMVFVAIPYYV
                     NAVREGFGRVPVQLENVARSLGASRARAFITVSLPLTTRHILYGALLAWGRAIGEFAA
                     VIMIAYFPVVISTLIYYRFNTGGIEESQAIAFILILLCLVLFFVFRILTRRAGVYDDR
                     S"
BASE COUNT          416 a          538 c          558 g          336 t
ORIGIN      
        1 atcgatcaga agagggaacg gacctgcgaa acggacctga tagccatggt cggtcgctac
       61 catggagatc acctgtcctt cgatacgatt gacctgctca cttcgtgaga tatcagcccc
      121 ggcaagcagg atatcccgtg atcttataca ggccaggatg cgttcaccgg caggaaccat
      181 gccctcggct atgatgccgg cagtcccgat cgagagcagg gttcttccac tttcaaatgc
      241 gcccgaagtc ccctcgagca tgttctcaaa tcccgcgagc gcggcaatgg ttggatgaac
      301 ggggcgggag aatacggctg ctggtgtgcc agcctgcagg atccttccct tatccatcac
      361 caccacctgg tcggcaagac ggtatgcctc atcgcagtca tggctcacct ggaggatcgt
      421 gagcccgttc tccctctgga gagccttcac ctcgtccatg caggacaggc gcgcttccgg
      481 gtcgagcgca gcaaacggtt catcgaggag gagtgcagaa ggccgtgtcg caagggctcg
      541 tgcgagcgcc acccgttgtt tctctccgcc tgagagcgat ccggtatggc ggtgggcgag
      601 aggatggagt gaaaacgagg agaggagcgt attcaccgta tcggcccgtt cgcgtgaagc
      661 aatccctttc attttcagac cataggcaat gttctcctca acagtgaggt gcgggaacag
      721 ggagtagtcc tggtacatga acccgattcc ccttttctct ggtggcagta tcgtgacgtc
      781 ccgtcccgct accaggatcc tcccgtcggt gacctgcagg aatcccccga tactctcgag
      841 aagcagggtc tttcctgcac ccgaaggccc catgagcacc gtataagagg attcgggaac
      901 ctggagcgaa atccttgcaa atgagaagtc cccgcggatc agactgactc cctcaactgc
      961 gatcatcata cacccctgca cggcgtgtca ggatccggaa tacgaagaag aggaccaggc
     1021 agagcaggat gaggatgaat gcgatggcct ggctctcttc gatcccgccg gtattgaagc
     1081 ggtagtagat cagggtcgat atcaccaccg ggaagtaggc gatcatgatc acggcagcaa
     1141 actccccgat ggctcttccc cacgcaagga gtgcgccata caagatatgg cgggtggtaa
     1201 ggggcaggct cactgttata aacgccctgg cccgggaggc accaaggctc cgggcgacgt
     1261 tctcgagctg gaccggcacc cttccgaatc cttcccgcac ggcgttcacg tagtagggga
     1321 tggcgacaaa gaccatggca gcaacgatgc ctgggaatgc gtcctcgaaa gtcaggccga
     1381 tcccgccgag cggagccccg agcagccccc tggacatgaa aagaaggtag accatcagcc
     1441 ctgcgaccgt atgcggcagg accagcggaa tatccaccag ggtctcaacg atgcccttcc
     1501 agggagtatc gcttcggctg agtgcatacg caagcggtgt cccggtgatg gcaaggatcc
     1561 cgaccgcaca gaaccctgca aggaaggtga caaagaaggc ctggaatacc attgggtcgc
     1621 tggcggctcc ggcaaggtat tccgggttct ggaaaaccgg gacggctatc acgagaaggg
     1681 ccagggcgat gatcccgaca aggatcatcc ccgggatcca gaaagcggca agagaatgac
     1741 cagaagacca gtggttcttc ttgagagaat gctccggtga gctcatgaga cagtcccttc
     1801 cgggagggga gcgggccacc cggggcgtcc ttttgccgac tcggccgc
//
LOCUS       DAOOCO010000267         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_69109_length_1844_cov_3.105646, whole genome shotgun sequence.
ACCESSION   DAOOCO010000267 DAOOCO010000000
VERSION     DAOOCO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69109_length_1844_cov_3.105646"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK154_10495"
     CDS             <1..320
                     /locus_tag="PK154_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW11527.1"
                     /translation="KTTKENLQGSISGETHEYTEMYPAFVKEAEAEKKSDAGLVFTYA
                     MKAEEVHATLYKEAMNALNHGQDMSNRAVILCPVCGNIFLGQAPEKCPICNAVRKVFK
                     TIE"
     gene            546..1694
                     /locus_tag="PK154_10500"
     CDS             546..1694
                     /locus_tag="PK154_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW11528.1"
                     /translation="MPQVTVYSTQNCPYCRMVKAYLERHGVDYLTIDVGTDKEQAEKM
                     IAISGQYGVPVITIDDEVIVGFDAQRLNELFGEEKGGEVYDVLIIGAGPAGLTAGVYC
                     ARKLLKTMIISENIGGQASESWAIENYMGYRMITGDDLMQKFEEQVRSLDLHLEIDRV
                     MILDRSGNEFVVNTATGAAYRARAVILAQGKQPRKLGVEGEDRYLGRGFSVCSTCDGP
                     LFRDKVVAIVGGGNSALQSAIEMSGIAREVHLVVRSEIKADEAYVRAYEKKANIVTHK
                     NSVVSKLQGNSMLHGITLKDRTSGKETDIPVDGVFAEVGWIPNTAFLEGFVALNPEKE
                     VVVDIDCHTSVPGIFAAGDITNVRTKQIITAAGEGAKAALSAYDYLIK"
BASE COUNT          410 a          492 c          539 g          403 t
ORIGIN      
        1 tcaagaccac caaggagaat ctccagggat ccatttctgg tgagacccat gaatataccg
       61 agatgtatcc cgcgttcgtc aaggaggccg aggcagagaa gaagagtgat gcgggcctcg
      121 tgtttaccta cgccatgaag gccgaggagg tgcatgccac cctgtacaag gaggccatga
      181 atgccctgaa ccatggccag gatatgtcaa accgggcggt catcctctgc ccggtctgcg
      241 gcaacatctt cctggggcag gctcctgaaa agtgcccgat ctgcaatgcg gtcaggaaag
      301 tgttcaaaac catcgagtga tacgtattgc cgtatcatcc ccctctgttt ttttctcccg
      361 gtttcctgcc tgcagaagtc ctcttatttt ccagccctct gaagtcctct tatccgtggg
      421 atgctgggat cgtcgtcggg accctgccat ttcagcatca gcttgatact tctcatctcc
      481 ggtcattctt ttcccccaat cctgctccca cactttaata accggtcagg agcaacctcg
      541 attgcatgcc gcaggttact gtgtactcga cccagaactg cccgtattgc cgtatggtga
      601 aggcctatct ggaacggcac ggggtggact atctcacgat cgatgtcggg acagacaagg
      661 agcaggccga gaagatgatc gcgatctcgg gacagtacgg cgtcccggtc atcacgatcg
      721 acgatgaagt cattgtaggc tttgatgcac aacgcctgaa tgaactgttt ggggaagaga
      781 agggcgggga ggtctacgat gtcctgatca tcggtgccgg gccggctggc cttaccgctg
      841 gggtatactg tgcgaggaaa ttattgaaaa cgatgatcat aagcgaaaat atcgggggcc
      901 aggccagcga atcctgggcg atcgagaatt acatgggata ccggatgatc accggtgacg
      961 acctgatgca gaagttcgag gagcaggtcc ggtcccttga cctccatctc gagatcgaca
     1021 gggtgatgat ccttgaccgg agcggcaacg aattcgtggt gaataccgcg accggagccg
     1081 cgtaccgggc ccgtgcggtc atccttgcac aggggaagca gccgcggaaa ctcggagtcg
     1141 agggggagga tcggtacctt ggcaggggtt tctcggtctg ttcaacctgc gatggtccgc
     1201 tcttcaggga caaggtggtc gccatcgtcg gtgggggcaa ttctgcgctc cagagtgcga
     1261 tcgagatgag cgggatcgcc cgggaagtgc accttgtggt gaggagcgag atcaaggccg
     1321 atgaggccta tgtcagggca tacgaaaaga aggccaacat cgtcacccac aaaaacagtg
     1381 tcgtgtcaaa actccagggc aacagcatgc tccatggaat caccctgaag gaccggactt
     1441 ccggaaagga gacggacatt cccgtggacg gggtgtttgc cgaggtgggt tggataccaa
     1501 acacggcgtt tttggaggga ttcgtggccc tgaacccgga gaaggaggtg gtcgtcgata
     1561 tcgattgcca taccagtgtt cccggcatct ttgctgcagg tgatatcacc aatgtcagga
     1621 caaaacagat catcaccgca gcgggtgaag gggcaaaggc ggccctctcg gcgtatgatt
     1681 acctgatcaa gtaacctccc cggtcttctt tttttcaggg tatagtggca tttcagccac
     1741 tcatttttcc accgcttttc gatcactatc ctcatttaag acgctcctgg gggttctcgc
     1801 cttcggctta cccccgccgc tggggcgtcc cccacaatgc gata
//
LOCUS       DAOOCO010000268         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_70033_length_1830_cov_2.754366, whole genome shotgun sequence.
ACCESSION   DAOOCO010000268 DAOOCO010000000
VERSION     DAOOCO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70033_length_1830_cov_2.754366"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK154_10505"
     CDS             complement(<1..216)
                     /locus_tag="PK154_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW11529.1"
                     /translation="MNLEIHDLSRNFPVPGQEPIQALSGITLTVKDKEFLSILGPSGC
                     GKTTLLRIIGGLDSASSGSITLNGASIN"
     gene            complement(259..1026)
                     /locus_tag="PK154_10510"
     CDS             complement(259..1026)
                     /locus_tag="PK154_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11530.1"
                     /translation="MRSPRVLLLPVLLIIGWEVAAYFIDNPFILPSLTTVIPILLHPF
                     STEYTLGTGSLVQNAAASLFRVGSGFLLAAAIAIPLGIGMGRSVFLHDFLDTTIELLR
                     PIPPLAWVPLALAWFKIGFVSITFIIFIGAFFPILLNTMDGVKSVKKTWIEVAETLGA
                     REQDVLTKVILPGSAPTVWTGMRVGFGVAWMCVVAAEWMPGISLGLGYLILYAYNFGQ
                     TNLIIAGMVVIGVIGILIDQVFRAGERRWFSWRSLER"
     gene            complement(1039..>1830)
                     /locus_tag="PK154_10515"
     CDS             complement(1039..>1830)
                     /locus_tag="PK154_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW11531.1"
                     /translation="AGKGNFTYGNITVSSVEVLQRAFPTVKFVNEPTEEWMNDNLEFL
                     YAQRELGVLIGSLANSTDNESLDLLFDTRPYDSAQAMIQAGTITTPAVQKDPVSVGYL
                     MSDHDAALFVAVKNWKYFEDTYGITIKPRDLAASRPDVADLFVNGEKVAEIRLVPADA
                     GPQLMQLASTNTIQYALVGNPPTIAAVDKGTPVKIIMSLNEEGSGIVIAEDAPANDWG
                     SFITWAKARSASNQPLKIAAPGKGSIQDVLLRYSLEESGLSVKEG"
BASE COUNT          419 a          485 c          506 g          420 t
ORIGIN      
        1 gttgatgctc gccccgttca gggtgattga acccgagctt gcggagtcaa gccccccgat
       61 tatccggagg agcgttgttt taccgcaccc tgaaggtccg aggatggata aaaattcctt
      121 atccttgacc gttaaggtga tcccggacaa agcctggatc ggttcctgtc ccggcaccgg
      181 gaagttcctg gaaagatcat ggatttcaag gttcatgtgc gatatgctcc tttcgcatag
      241 tgaatacctg caactccatc accgttccag cgatctccac gagaaccatc ttctctcacc
      301 agccctgaag acctggtcta tcaggattcc gatcaccccg atcaccacca tccctgcgat
      361 gatgaggttg gtctggccga aattgtatgc atagaggatg aggtatccca gtccgagaga
      421 aattcccggc atccattcgg cggcaacaac acacatccat gcgactccga atcctacccg
      481 catcccggtc cagaccgttg gcgctgagcc gggcaggatc acttttgtga gcacgtcctg
      541 ttcccgggcg ccgagagttt ctgccacttc gatccaggtc ttcttgacag attttactcc
      601 atccatcgta ttgaggagga tcggaaagaa tgccccgatg aagatgatga aggtgatgga
      661 gacgaatccg atcttgaacc atgccagggc aagaggtacc catgcgaggg gagggatcgg
      721 ccgcaggagt tcgatcgtgg tatcaagaaa atcatggagg aacaccgatc ggcccatgcc
      781 aatcccaagg ggaatggcga ttgctgccgc aagaagaaat cccgatccta cccggaaaag
      841 gcttgcggct gcattctgga caaggctgcc ggtcccgagg gtgtattccg tagagaaagg
      901 atggaggagg atggggatga cggttgtcag ggaggggagg atgaacgggt tgtcgatgaa
      961 atacgctgcc acctcccatc ctatgataag aaggacaggg aggagcagga cacgggggct
     1021 cctcattatc ctgatagatt acccttcctt cacagataaa ccactctctt ccagggaata
     1081 gcgaaggagc acatcctgga tagatccctt ccccggtgct gcaatcttca gcggctggtt
     1141 gctggcagag cgggcctttg cccatgtgat gaagcttccc cagtcattgg caggggcatc
     1201 ttctgcaatg acaattccgg atccctcttc attgagcgac atgatgatct ttaccggggt
     1261 tcctttgtcg accgctgcaa tggtgggagg attgccgacc agggcatact ggatagtatt
     1321 cgtacttgca agttgcatca actgcggccc tgcgtcagca gggacaagcc ggatctctgc
     1381 tactttctcc ccattcacga agagatcagc gacatcaggg cgggatgcgg ccagatcgcg
     1441 cggcttgatg gtaattccgt aggtgtcttc aaagtacttc cagttcttga ccgcgacaaa
     1501 cagtgcagca tcgtgatcgg acatcaggta tcctaccgat acagggtctt tctggactgc
     1561 aggagtggtg atcgtccctg cctgtatcat tgcctgtgcc gaatcatacg gcctggtgtc
     1621 aaagagcagg tcaagtgact cgttgtcggt gctgtttgca agggatccga tcaggacacc
     1681 aagttccctc tgcgcataca ggaattcaag gttatcattc atccactctt ctgtgggctc
     1741 gttcacgaat ttgacggtgg ggaatgcccg ctggagcact tccacggaag aaaccgtgat
     1801 atttccatag gtgaagttgc ctttcccggc
//
LOCUS       DAOOCO010000269         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_70730_length_1818_cov_4.160522, whole genome shotgun sequence.
ACCESSION   DAOOCO010000269 DAOOCO010000000
VERSION     DAOOCO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70730_length_1818_cov_4.160522"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PK154_10520"
     CDS             <1..1768
                     /locus_tag="PK154_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPW11532.1"
                     /translation="HQWSSFFRGLSFIVIDEAHRYRGVPGSQMAFLIRRLLRLCRHYG
                     SDPRCFLSSATLANPLEFAERCCGLPFTLIEGDSAPRGPRHFVMINPYDDPDRPSSLN
                     KEAADLITECIRHRIQSLVFTGSRKTAELVALRARERMVRTRQGRPEELCSYRAGYLP
                     EERRMIEERLKSGDLRCVVSTNALELGIDIGSLDAVIIAGYPGTMMATWQQAGRAGRN
                     GTPSAAFLIGGGDPLDQYFLRHPDLFFGAPLERAIIDVGNPYILSGQILCAAAELPIT
                     PENIHLFGTETGPAIDALLQERLISKTPGGLVYSGSRRPHELIRIGSISPDSFRVVTE
                     GRTLETLDRNQAFREAHQGAVLLHQGDQYLVDSMDMAHGIIRVSKTEVDYYTRPLKSV
                     EIAVREVIETKTIHDVPVSFGDVEVTEQYYAYKIIRGDTLLGVEALDLPPLAFSTKAL
                     WFSPADRTGAEIQCAGRDPDGGLHGAEHAFIAMMPLYVLCDRWDIGGVSIPMHKEAEK
                     PVVFIYDGYEGGIGLSERAYHLFEEIAAHALRLVEECPCETGCPSCIFSPKCGNDNQP
                     LDKRATMTILKSLCLSGHPDGG"
BASE COUNT          390 a          518 c          507 g          403 t
ORIGIN      
        1 ccaccagtgg agttcattct tcaggggtct ctccttcatc gtgattgatg aagcccaccg
       61 ataccgcgga gttccgggtt cccagatggc ctttctgatc cggagacttt tgcgcctgtg
      121 ccggcattac gggtcagatc cccgttgttt cctttccagc gcaacccttg ccaatcccct
      181 ggagtttgcc gaacggtgct gcgggcttcc gtttaccctt attgaaggag acagtgcacc
      241 ccgcggaccc aggcattttg tcatgatcaa tccttatgac gatccggacc gtccttcgtc
      301 cctcaacaag gaagccgctg acctgatcac cgaatgcatc cgtcaccgca tccagagcct
      361 cgtatttacc ggatcacgga agactgccga actggttgcg ctccgggcga gggagaggat
      421 ggtccggacc aggcagggga gaccggagga gctctgttcc taccgtgcag ggtatctgcc
      481 cgaagagcgc cggatgatcg aagaacggtt gaaatcagga gatctgcgat gcgtggtatc
      541 gacaaacgcc ctcgagcttg ggatagatat cgggtccctt gatgccgtca tcattgcagg
      601 gtatccaggg acgatgatgg cgacctggca gcaggccggg cgggccggca ggaatggaac
      661 cccctctgct gcattcctga ttggcggcgg agatccactc gaccagtact tcctgcgtca
      721 ccccgatctc ttctttggtg cccctctcga gcgtgcgata atcgacgtgg gaaatccgta
      781 cattctctcc ggacagatcc tctgtgctgc agcagagcta cccatcaccc cagagaatat
      841 ccaccttttc ggcacggaga ccggtccggc aattgacgcc cttctccagg aacggctcat
      901 ctcaaaaacc cccggggggc tcgtctattc tgggagcagg agaccgcacg aactcatcag
      961 gataggaagc atttcgcccg actccttcag ggttgtgacg gaaggccgga cactcgagac
     1021 tctggaccgg aaccaggcgt tcagggaggc ccaccaggga gcagttctcc ttcaccaggg
     1081 tgaccagtat ctcgttgact ccatggatat ggctcacggt atcatccggg tcagcaaaac
     1141 tgaggttgat tattacaccc gacccctgaa atcagtggag atcgccgtcc gcgaagtcat
     1201 cgagacaaaa accatccatg atgtccccgt cagtttcggt gatgtcgaag tgaccgagca
     1261 gtattatgca tataagatta tccgtgggga tacccttctt ggggtggagg ctctggatct
     1321 tccccccctc gccttttcta caaaggccct ttggttctcc cccgctgaca ggaccggagc
     1381 ggaaatccaa tgtgcagggc gtgatcccga tggcgggctg cacggtgctg aacacgcatt
     1441 tatcgccatg atgccattgt atgtcctgtg cgaccgatgg gacattgggg gtgtttctat
     1501 cccgatgcac aaagaggccg aaaagccggt agtgttcata tacgacggat acgaaggcgg
     1561 aatcgggctt tcggaaaggg catatcatct gtttgaggag attgccgccc atgctctccg
     1621 tcttgttgaa gagtgcccct gcgaaacggg atgtccttcc tgtatctttt cgcccaagtg
     1681 cggcaacgat aaccagcccc ttgacaagcg agcaacgatg acgatcctga aatcactctg
     1741 tctgagcggg cacccagatg gagggtagtg tacacgaagt gatgtaaaat tggaaaaggc
     1801 cctgtatcct actcagtt
//
LOCUS       DAOOCO010000270         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_71782_length_1802_cov_4.080137, whole genome shotgun sequence.
ACCESSION   DAOOCO010000270 DAOOCO010000000
VERSION     DAOOCO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71782_length_1802_cov_4.080137"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK154_10525"
     CDS             <1..242
                     /locus_tag="PK154_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW11533.1"
                     /translation="LVLPRDLAGSDLVMQFIGERVSREAYVNIMDQYQWNSSVSSPDF
                     IEKYPGFEALLRGITGEEYGYAIRCARKYGLHRGF"
     gene            395..613
                     /locus_tag="PK154_10530"
     CDS             395..613
                     /locus_tag="PK154_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11534.1"
                     /translation="MGSMKGYTDYKRREYCNDVQCPVQLLLNKEMEKSPKYEEIRAIC
                     ASHCLHSTHEFHHWLTEKGYLIVRPKGE"
     gene            664..1488
                     /gene="lsrF"
                     /locus_tag="PK154_10535"
     CDS             664..1488
                     /gene="lsrF"
                     /locus_tag="PK154_10535"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HPW11535.1"
                     /translation="MVQAYLDVGIQSRMNRIISPKDNRVVMLAVDHPYFQGPTTGLRN
                     MSRTIQTLLPSCDCLMTTRGAVRSNIAPGDLGSKPIMLRVTGGNSVLFEELSDEKLRV
                     TIREAIRMDAAGVAVSVYVGSANQQQTILNLTDMINHAEEYGMPVLAVTAVGKNMARD
                     LRYLALASRICQDAGARIIKTYYCEEFSKLVDWVAPTAVVVAGGKYSSPPDALQMAYH
                     SVQAGAAGVDFGRNIFQDENPLGMIKAIKAIVHEDHTVKEALDIYHACLPHASKLD"
BASE COUNT          442 a          467 c          491 g          402 t
ORIGIN      
        1 acctggtcct tcccagggac cttgccggat cggacctggt gatgcagttc atcggggagc
       61 gggtatcacg ggaagcgtac gtgaatatca tggaccagta ccaatggaat tcgtcagtgt
      121 catcacctga ttttatcgag aagtatccgg ggtttgaagc actcctacgc ggtattacgg
      181 gagaggagta tggatatgcg atacggtgtg cgaggaagta cgggctgcat cggggatttt
      241 gacgagggag cctctcgtca gaaccatcga tcagtcgggc aaaccggacc gctgtcatct
      301 ctccgtcccg gtgtgtgcag taaaaaaggg gaaagttccg cacccatcct ttctcttcca
      361 taaacctttt tccgttcatt tctgagtacc tattatggga tcgatgaagg gttataccga
      421 ttataaacgc cgggagtatt gtaatgacgt gcaatgcccg gtgcagctgc tactgaataa
      481 agagatggaa aaatctccca aatatgaaga gatccgggcg atttgtgcat cccattgcct
      541 gcacagcacc catgaatttc accactggct gaccgagaaa ggatacctga tcgtcaggcc
      601 gaagggagag taggaccaac tatttttagc gttgtgaatc attacttctc gtgagatgtc
      661 cccatggtac aggcatacct tgatgttgga attcagagcc ggatgaaccg gatcatcagt
      721 ccgaaagata accgggtggt tatgcttgcc gtcgaccatc cctatttcca ggggccgacg
      781 acaggccttc gtaacatgag caggaccata caaacactcc ttccctcctg cgattgcctg
      841 atgacaaccc gtggggcagt ccggagcaat atcgcacccg gagatctcgg gtcaaaaccc
      901 atcatgctgc gggtgacggg gggaaacagc gtcctattcg aggagttgag cgacgagaag
      961 ctcagggtca ccatcaggga agccatccgg atggatgcag ccggggtggc ggtcagtgtg
     1021 tatgtcggct ctgccaacca gcagcagacg atcctcaacc tgaccgatat gatcaatcat
     1081 gctgaagagt acggaatgcc ggtcctggct gtgaccgccg ttggtaaaaa catggcaagg
     1141 gatctccgct acctggcact tgcctcccgc atctgccagg atgccggggc ccggatcatc
     1201 aagacctatt attgcgagga attttccaaa ctggtcgact gggttgctcc cactgcggtt
     1261 gtcgttgccg gagggaagta ctcaagtccc cctgacgctc tccagatggc ctaccactcc
     1321 gtccaggcag gtgccgccgg agtcgatttc gggcggaata tattccagga tgaaaatccc
     1381 ctcggtatga tcaaggcgat caaggcaatt gtccatgaag accacaccgt gaaggaagcc
     1441 cttgatatct accacgcatg cctgcctcat gcctcaaaac tcgactaatt ttttgggatt
     1501 tcgagaattc cctaggcacg attatcggaa aaataacact caatgtggat tgaagtgaat
     1561 ctgccgagac acacgcatat ataagcataa tggcaaaccc atttttgtaa accgtcaatg
     1621 gttgccgtcc ggccgggcag aaagcaggaa actgccgggg gcagtttcac gggaagccat
     1681 cattcgcgaa gccaggtcca ggccttgctg atatcggcgg actggaaaaa cctggcatcg
     1741 gttgcatacg cagctttggc cagatgcgtt agccacttct cccaggtcgt gtcgccaacg
     1801 ac
//
LOCUS       DAOOCO010000271         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_76780_length_1729_cov_3.848268, whole genome shotgun sequence.
ACCESSION   DAOOCO010000271 DAOOCO010000000
VERSION     DAOOCO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76780_length_1729_cov_3.848268"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..814)
                     /locus_tag="PK154_10540"
     CDS             complement(29..814)
                     /locus_tag="PK154_10540"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11536.1"
                     /translation="MAEPVLSISGIEKTFRGESESCPALSGVSFDIAAEEIICIMGPS
                     GCGKSTLLRIIGGLEHEDKGSIHTRAEPLGEGVSAAMIFQDHALFPWLSVEENIIYGL
                     RLSAQKVPDTEVHRRVDDLLSLIHLTSFARSLPHELSGGMKQRVSVARALAIRPSILL
                     MDEPFSALDIFTRRELEDEILRIRQELKTTILMVTHNPEEAVYIADRIIILSERPGVV
                     AGIVTVPLPHPRSPADPDFIRIREEVAGRVKGKNVVRCGIQHG"
     gene            complement(814..1599)
                     /locus_tag="PK154_10545"
     CDS             complement(814..1599)
                     /locus_tag="PK154_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11537.1"
                     /translation="MVLISKQKVREYGKGLAIIILLLVAWQCAALVIDNNYILPSLTE
                     IADVLLHPLQPVLGGESLIANTMVSLKEVLTGFFCAAALAIPLGLLIGWSSEVQAYLN
                     PATQILRPIPPIAWMPFAIAWFGIGINSILFIIALGAFFPILINTVDGVKGIRVRWIE
                     VAEMFHATTFEKLRTVVLPGALPRIWTGLRVSFAIAWMCVVAAEMLPGTTAGLGFLIM
                     YAYNLGQLDVIGAGMVVIGIIGLGADMLFRAGQDRFFSWEGKD"
BASE COUNT          398 a          485 c          469 g          377 t
ORIGIN      
        1 atcatcttgt aacggggaga ggttctggtc atccgtgttg aatcccacag cgtacaacat
       61 ttttcccctt taccctccca gccacctcct cgcggattct gataaagtcc ggatcggctg
      121 gactcctggg gtggggcagt ggtaccgtta cgataccggc aaccaccccc ggcctttcag
      181 agaggatgat gatccggtcg gcgatgtaca ctgcttcctc ggggttgtgg gttaccatga
      241 gaatagtggt cttcagttcc tgccggattc ggagaatctc atcctcaagt tccctccggg
      301 taaagatatc gagggccgag aacggttcgt ccatcaggag gattgacggt ctgatcgcaa
      361 gcgcccgtgc gactgagact cgttgtttca tgcctcccga gagctcgtgc gggagtgaac
      421 gggcaaatga tgtgaggtga atcagcgaaa gaaggtcatc aacccggcga tgcacttcgg
      481 tatctgggac cttctgggca gagaggcgca ggccatatat gatgttctct tccaccgata
      541 gccacgggaa gagtgcatga tcctggaaga tcatggctgc cgatactccc tctcccaagg
      601 gctctgctct cgtgtggatt gagcccttat cctcatgttc gagccccccg atgatccgga
      661 gcagggtgga ttttccacat ccggaaggac ccatgataca gatgatctct tcggctgcga
      721 tgtcaaagct gacgccggaa agggcagggc aggattcact ctcaccacgg aatgtctttt
      781 caatgccgga gatcgagagg acaggttcag ccatcaatcc ttcccctccc atgagaaaaa
      841 tctgtcctgt cctgccctga agagcatgtc agcaccaagg ccgatgatcc cgatcaccac
      901 catcccggct ccgatcacat cgagctgtcc gagattgtag gcatacatga tcaaaaatcc
      961 gagacctgcg gtagttcccg gcagcatctc ggcagccacc acgcacatcc acgcgatggc
     1021 aaacgagacc cggagcccgg tccagatccg gggaagggct ccgggaagga ccaccgtccg
     1081 cagtttttcg aaggttgtgg catggaacat ctctgcaacc tcgatccacc tcacccgtat
     1141 tcccttgact ccatctaccg tgttgatcag gatcgggaag aatgcaccaa gtgcgatgat
     1201 gaacaggatc gagttgatcc caatcccgaa ccatgcaatg gcaaacggca tccatgctat
     1261 cggggggatc ggtcggagga tttgggtagc agggttgagg tatgcctgca cctcagatga
     1321 ccacccgatg aggaggccca gcgggattgc cagtgcagcc gcacagaaaa atcctgtgag
     1381 cacctccttc agacttacca tcgtgtttgc aatcaggctc tctccgccga gcaccggttg
     1441 aagtgggtgg agcagcacat cggcgatttc ggtgagcgag gggaggatat aattgttgtc
     1501 gattaccagt gcagcacatt gccatgccac gagcaggaga atgatgattg ccagcccttt
     1561 tccgtattca cgaaccttct gttttgaaat cagtaccata gaacgtcacc gtgatatttt
     1621 cgtatgcaca tgcctcgcga accatctctt cctgaatgga gctctggacg gtagcaataa
     1681 tcaccggcct gccggcagca gaacgaccct ttgcccaaag gacaaaatc
//
LOCUS       DAOOCO010000272         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_78084_length_1712_cov_4.497284, whole genome shotgun sequence.
ACCESSION   DAOOCO010000272 DAOOCO010000000
VERSION     DAOOCO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78084_length_1712_cov_4.497284"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1234
                     /locus_tag="PK154_10550"
     CDS             248..1234
                     /locus_tag="PK154_10550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW11538.1"
                     /translation="MNNDIPHRVIHGGKVRQFQQEHGKSAIDFSANLNPWPPAVPLSL
                     PDDLISSYPDDRYLLLKEAIAERFHRPVEEIAVGNGSIELIRVFCQATLSRGDHVWIR
                     NPTFGEYEMSARLAGAIPAGEWRPAAAGFLCNPNNPTGELQSRQAVLDLLSPAKILFV
                     DEAFIELSDINQSVADVRDPSLFICRSLTKSFSVPGIRFGYGFGDPDLIERMEIIRPP
                     WSVNSIAEQFAIRAFSRYEQLEQSRSRIRAEREWLISALRTLPLSVHPSDTNFLLLSF
                     PYDVAPLCSALSVRGILVRDCHSFGLPSSIRVAVRTRGENRLLLEAIGECMR"
     gene            1222..>1712
                     /locus_tag="PK154_10555"
     CDS             1222..>1712
                     /locus_tag="PK154_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPW11539.1"
                     /translation="MYALILAGGSGTRLNLGEKPLVTLNGKPIIEYVIKAFHDAGYEV
                     VVILTGKTPHTLNWCRARAIPYLVASGAGYIEDLAEAAEYLEIRDPFFSCVADLPCLS
                     PATIREIREHYQKSGKEACSVWVPEELIRNTGSHPSYSGCIGPILACPAGINILRWDL
                     MEKP"
BASE COUNT          390 a          502 c          430 g          390 t
ORIGIN      
        1 ctgaagcatc tcgatctgat ggtcgggaag cctgagcgta attcgctgca ttttttactc
       61 ctcatgtatg acaattgtca gacaattgtc tgctaatcat ctgtcagaga gctaccttta
      121 gtctgacatt agatcagatg attcctaaat aaacagcaat agtatatata ttttttcttt
      181 ctcgtcccaa ataccttctg cataccttaa taccccggtg cacccatttc tgaagggaaa
      241 tttgagcgtg aacaacgata tcccccacag agtaatccac ggcggaaagg tccggcagtt
      301 ccagcaggaa catgggaaaa gtgccatcga tttcagtgcg aatcttaatc catggccccc
      361 agctgtcccc ctctctctac ctgacgatct catttccagc tatccggatg accgatatct
      421 cctcctcaag gaggctatcg cggagaggtt tcaccggccg gttgaggaga tcgcggtagg
      481 aaacggatcc attgaactga tcagggtctt ctgtcaggcg accttatccc gtggagacca
      541 cgtatggatc aggaatccca cctttgggga gtatgaaatg tcagcacgcc tggcaggcgc
      601 catccctgca ggggaatggc gaccagcggc agccggattc ctctgcaatc cgaacaatcc
      661 caccggagaa ctccagagca ggcaagccgt ccttgatctg ctctcccctg caaagatcct
      721 ttttgtcgat gaagcattta tcgagctctc cgacatcaac cagagcgttg ccgatgtcag
      781 ggacccctcc ctattcatct gccgatccct cacgaagagc ttctcagttc ccggaatccg
      841 gtttgggtac gggttcgggg acccagacct catcgaacgg atggagatca tacgacctcc
      901 ctggagtgtc aactcgatcg ccgagcagtt tgcaatcagg gcatttagca ggtatgaaca
      961 gctcgaacag tcccggtcca ggatccgggc agaacgggaa tggctcatct ctgccctccg
     1021 caccctcccc ctttcggttc atccctccga tacgaatttt ctgctcctga gcttcccgta
     1081 cgatgtcgca cccctctgct ctgctctctc cgtaaggggc atcctcgtcc gggactgcca
     1141 ttcattcgga ctccccagtt ccatccgggt agcggtccgg acgagggggg aaaaccgcct
     1201 gctcctggag gcgatcggtg aatgtatgcg ctgatacttg cgggagggag cggaacacgg
     1261 ctcaatcttg gtgaaaaacc acttgtcacc ttgaacggaa agccgatcat agaatacgtt
     1321 atcaaagcct tccatgacgc agggtacgag gtcgtggtga tcctgaccgg aaagactccg
     1381 cacaccctca actggtgcag ggcacgggcc atcccctatc tcgtcgcatc aggagccggg
     1441 tatatcgaag atcttgccga agcggcagag tatctcgaga taagggatcc tttcttttcc
     1501 tgtgttgccg atctcccctg cctcagcccg gcaaccatcc gcgagatcag ggaacactac
     1561 cagaaatccg gaaaagaggc ctgctcggta tgggtgcccg aggagctgat caggaacaca
     1621 ggctcccacc cttcgtattc gggatgcatt ggccccatcc tggcatgtcc ggccggcatc
     1681 aacatactcc ggtgggatct catggagaaa cc
//
LOCUS       DAOOCO010000273         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoregulaceae archaeon isolate AMR_MDS_4016
            NODE_93848_length_1534_cov_4.878296, whole genome shotgun sequence.
ACCESSION   DAOOCO010000273 DAOOCO010000000
VERSION     DAOOCO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865987
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregulaceae archaeon (wastewater metagenome)
  ORGANISM  Methanoregulaceae archaeon
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:39:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,113
            CDSs (total)                      :: 2,075
            Genes (coding)                    :: 2,068
            CDSs (with protein)               :: 2,068
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Methanoregulaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93848_length_1534_cov_4.878296"
                     /isolate="AMR_MDS_4016"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2485498"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK154_10560"
     CDS             complement(<1..831)
                     /locus_tag="PK154_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPW11540.1"
                     /translation="MAIMKIRGGDLDLVEYEFISFHPGENIRTLGLERSYPLSPVKGT
                     VTVRSPARIHLTVFDMNRFAPGRAGGGGLGFALQLYCSVDVSCTESGIEIDYSREGII
                     RHFVEVFRKVTGYKGGFRIRARDHQHQHVGLGSTSTVMISLANALNYAVGSPLTPDQL
                     RKLVGNNYVEETAGETIAFAFETGVGPAVSTYGGMAIMGDELTLAYHHPFAEGKNVFV
                     VIPPSDISSAGTKEFDLLMNKARVLDYRDRELKAYLMLMDMIPALEEDNLKRIGNIIW
                     E"
     gene            complement(1168..>1534)
                     /locus_tag="PK154_10565"
     CDS             complement(1168..>1534)
                     /locus_tag="PK154_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW11541.1"
                     /translation="PLFMVPMWVIILSAAAIALGTSFGGWKVINKLAKEITKIRPYQG
                     FCAATAGGAVLAMVTTYGIPVSSTQVMSGSIVGVGATRGVKAVKWEVTRRMMEAWVVT
                     IPLAFALSWSAYVVLSWFL"
BASE COUNT          340 a          445 c          403 g          346 t
ORIGIN      
        1 ctcccagatg atattgccga tccgcttcag gttgtcctcc tcgagcgccg ggatcatgtc
       61 catgagcatg aggtacgcct tcagttccct gtcgcggtag tcaagcactc ttgccttgtt
      121 catcaggagg tcaaactcct ttgttcccgc agatgagatg tcacttgggg ggatgaccac
      181 aaagacattt ttcccttccg cgaatggatg gtggtaggcc agcgtcagct catctcccat
      241 gattgccata ccgccgtaag tactgacagc agggccgaca ccggtctcga atgcaaaggc
      301 gatcgtctcc cctgcggtct cttcgacgta attgttgccg accagtttcc tgagctgatc
      361 cggcgtgagg ggagatccca cagcatagtt gagtgcgttg gctagcgaga tcatgacggt
      421 actcgttgaa ccgagcccga catgctggtg ctggtgatcc cgggcacgga tccggaatcc
      481 tcccttgtac ccggtcactt tcctgaacac ctctacaaaa tgccggatga tgccttcacg
      541 ggagtaatca atctcgattc ccgattccgt gcaggagaca tcgacgctgc agtagagctg
      601 gagggcaaac ccgagccccc cgcctccagc ccgtcccggg gcaaaccggt tcatatcaaa
      661 aacggtcagg tggatccgtg ctggtgaacg gaccgtgacc gtgcctttta caggagaaag
      721 gggataggaa cgttcgagcc caagggttcg gatattctct ccgggatgaa acgagatgaa
      781 ctcgtattcg acgagatcga gatctcctcc ccgaattttc atgatggcca tctacataac
      841 tctcctttcc agtgaccgat tctgatcgat cagagcatat gtttcctgta gctccggggg
      901 gtcttaagga tgttcatcaa caaccggttc ggataattcc ggagtgtata tggtaaattt
      961 tccgaggttt acccctgtgt aatgtcaagg gggagagtgt caacccaatc cacttctctt
     1021 ctcagggatc ctctttgtca gcgcattgaa ccgaagccag gaaccttccc ccgagctcca
     1081 gtgtccatta gtcaccggca aagactcttc agggaacgac ccgtgtacct tctgtacccc
     1141 aaaaaggaag ccgggccctt ctacatccta gaggaaccac gagaggacca cgtaagcaga
     1201 ccaggagagg gcaaatgcaa ggggaatggt cactacccat gcctccatca tccggcgggt
     1261 gacctcccat tttaccgcct tgacacctcg ggttgcaccg acacctacga tcgatccgct
     1321 catcacctgg gtggaggata ccgggatccc gtaggttgtc accatcgcaa ggacagcccc
     1381 ccccgctgtt gcagcgcaga acccctggta tggccggatt ttggtgatct cttttgccag
     1441 cttgttgata actttccatc ccccgaaact cgtgccaagc gcgatcgcgg cggctgagag
     1501 gatgatcacc cacatgggga ccatgaacaa cggg
//
